LOCUS       DATDMP010000001         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_666575, whole genome shotgun sequence.
ACCESSION   DATDMP010000001 DATDMP010000000
VERSION     DATDMP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666575"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /gene="ggt"
                     /locus_tag="VI195_00005"
     CDS             complement(<1..1567)
                     /gene="ggt"
                     /locus_tag="VI195_00005"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6822791.1"
                     /translation="MTTISGRAACLGAAAAVTLIAWTTGYAAAPTPAAALATPDRYSA
                     DAGAEILGAGGNAADAAVAIAFTLAVTYPEAGNLGGGGFATVLFDGKADFLDYRERAP
                     ASASADMYLDAAGKVIPEASTVGAGAAGVPGTAAGIWELHHRFGRLKWQADLAPAIRY
                     AEQGFPVDRMLVEKRDARAAALRGRTNFLTYYGALAEGYTFRQPELAATLKRIATDGP
                     RGFYAGRTAELLLAEMAHSHGHMAATDLEAYRAVWRKPLEGEWAGYHLITAPLPSSGG
                     IMLLSMLAMKADLARAFAGVALNSAQYVHLVAEIEKRVFADRASYLGDPDFTKAPVAQ
                     LLDPSYLARRAAEINTERPTPTAQVKPGLAQHHDTTHFSVIDRFGNAVSNTYTLNDDF
                     GCGQVVTGAGFLLNNEMDDFSVKPDVPNIYGVVGGDANAIAPGKRPLSSMTPTILTQG
                     GQAAVIIGTEGGPSITTQVFQVLIAWHDLHMSLASAIAAPRVHHQLYPADTLIEEPYA
                     LLEPTVRTALIARG"
     gene            1892..>2932
                     /locus_tag="VI195_00010"
     CDS             1892..>2932
                     /locus_tag="VI195_00010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6822792.1"
                     /translation="MRSIVLCVTLCWCVWVSAEPASIQSSSFEAADRVFADFSLDAHI
                     PGLVYGIVAEGRLVHIGTFGVQDLDSRRPVTGDTLFRIASMTKAFTALTVLKLRDEGR
                     IRLDSLAEEYVPELRGWKYPTDDSPRIRVRDLLNHTAGFVTDDPWGDRQTPLPESEFT
                     RLLRNGVPFTRAPEIAYEYSNLGFALLGRVITNVSKQPYAETITDRLLRPLGMRSSGF
                     VADAAPLERRALGYRWQDDAWQLEPTLGPGAFGAMGGLQTSANDYAKWVTFLLSAWPA
                     RNGADTGVAARATVRELAQGSNFPRLRPRAGKNPEGCRQAVSYGMGMAVMMDCDLGFT
                     LTHAGGYPGFGSY"
BASE COUNT          481 a          891 c         1005 g          555 t
ORIGIN      
        1 agccacgcgc gatgagtgcc gtgcggactg tcggctcgag cagcgcgtag ggctcttcta
       61 tgagcgtgtc ggccgggtac aactggtggt ggacgcgggg tgccgcgatg gctgaggcga
      121 gcgacatgtg caagtcgtgc caggcgatga gcacctggaa gacctgtgtc gtaatgctcg
      181 gcccgccctc ggtgccgata atgactgcgg cctgaccgcc ctgggtgagt atcgtcggcg
      241 tcatgctgga gagcggccgc ttgcccggcg cgatcgcgtt cgcgtcgccg ccaaccaccc
      301 cgtaaatgtt cggcacgtcg ggcttgaccg agaagtcgtc catctcgttg ttgagcagga
      361 acccggcgcc ggtaaccacc tgcccgcaac cgaagtcatc gttcagcgtg taggtattcg
      421 acactgcgtt gccgaagcgg tcgatgaccg agaaatgtgt ggtgtcgtgg tgctgggcaa
      481 gtcccggctt cacttgcgcg gtgggcgtcg ggcgctcggt gttgatttcc gcggcgcgcc
      541 gcgcgaggta ggaggggtcg agcagctgcg ccaccggggc cttggtaaaa tccggatcgc
      601 cgagatagct ggcgcggtcg gcgaacacgc gcttctcgat ctcggccacg aggtgcacgt
      661 actgcgccga attgagcgcc acaccggcga acgcccgggc gaggtctgcc ttcatcgcga
      721 gcatcgagag cagcatgatg cccccagaac tcggcagcgg cgcggtgatg aggtggtagc
      781 ctgcccactc gccctcgagc ggcttgcgcc acacggcgcg ataggcctcg aggtcggtgg
      841 ctgccatgtg gccgtggctg tgcgccattt cggcgaggag tagctcggcg gtgcgtcctg
      901 cgtagaagcc gcgcgggccg tctgtggcaa tgcgcttgag tgtcgcggcg agttcgggct
      961 gacggaacgt gtaaccctcc gccagcgcgc catagtaggt aaggaagttg gtgcgaccgc
     1021 gaagcgccgc cgcgcgcgca tcgcgcttct cgaccagcat gcgatcgacg ggaaagcctt
     1081 gctccgcata gcggatggca ggggcgaggt cggcttgcca tttgagcctg ccgaatcggt
     1141 gatgcagctc ccatatcccc gcggcggtac ccggcacgcc ggcggcgccg gccccgacgg
     1201 tactcgcctc ggggatgact tttcctgcgg catccaggta catgtctgcg ctggcgctcg
     1261 cgggagcacg ttcgcgatag tcgagaaaat ccgccttgcc gtcgaacagc acggtcgcga
     1321 atcctccgcc gccgaggttc ccggcctcgg ggtaggtgac ggcgagggtg aaggcgatcg
     1381 cgaccgcagc atcggccgcg ttaccgccgg cgccgaggat ttcggctcca gcgtcggcac
     1441 tgtatcgatc tggcgtggcg agcgcggccg ctggggtcgg cgcggcggcg taccccgtgg
     1501 tccaggcgat cagtgtgacg gcggccgcgg cacccaggca tgccgcgcgg ccggaaatgg
     1561 tggtcataga cgcaccttgt acgcagcgct acgcctgttg gcctgtttgt tggggtccgc
     1621 aaggtccgct ctccccgtgc acaacctgga gttgatttcc gcatgcgttg ccggcgagtc
     1681 tgcgcgcatc gattggtgac gatcggaatc tcggtgcaga atcagcagct taggccgcgc
     1741 ttcgattccg tcacgtctca aatatccacc tctgaagaac tcttgcctcg gtccccagca
     1801 cctcttggca cggcggcgct cgcggtggag caaaatcgac cgcaggcggt cgctcgaagc
     1861 atcgctacgt tgtcgatacc gggggacgac catgcgttcc atagtgctgt gcgtaacgtt
     1921 gtgttggtgc gtgtgggtta gcgcagagcc cgcttccatc cagtcgagct cgttcgaggc
     1981 tgccgatcgt gtcttcgcgg attttagcct tgatgcacac attccagggc tcgtgtacgg
     2041 aatagttgca gagggtcgcc tggtgcacat cggcaccttc ggtgtccagg accttgactc
     2101 ccggcgaccg gtcaccggcg atacgctctt tcggatcgcg tcaatgacga aggcatttac
     2161 ggcgctaacg gttttgaagc tacgggacga ggggcggatt cggctcgact cgctcgccga
     2221 ggaatatgtg cccgagttgc ggggctggaa gtacccgacc gacgactcgc ccagaattcg
     2281 cgtacgcgac ctgttgaacc acacggcggg cttcgtgacg gacgacccgt ggggagaccg
     2341 tcaaacaccg ctgccggaga gcgaattcac gcggttgctg cgcaacggcg tgccgtttac
     2401 gcgcgcgccg gaaatcgcct acgagtactc gaaccttggg tttgcactcc tcggcagagt
     2461 catcacaaac gtctcgaagc aaccgtacgc agagacgatc accgatagat tgcttcgtcc
     2521 cctaggtatg aggtcgtctg gcttcgtagc ggatgccgcg ccgctcgagc gacgagcgct
     2581 gggctatcgt tggcaggacg acgcgtggca gctcgagcca acgctcggac cgggcgcatt
     2641 cggcgcaatg ggtggattac agacgagtgc caatgactat gccaagtggg tgacgttcct
     2701 gttgtccgcg tggcccgcgc gcaacggtgc agataccggt gtggcggctc gtgcgaccgt
     2761 tcgcgagttg gcacaaggct ctaacttccc gcgtcttcgc ccgcgggcag gtaagaaccc
     2821 ggaaggatgc cgccaggcgg ttagctacgg catgggtatg gccgttatga tggactgcga
     2881 tcttgggttc acgcttacgc acgccggcgg ttacccaggc tttggctcgt at
//
LOCUS       DATDMP010000002         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1555266, whole genome shotgun sequence.
ACCESSION   DATDMP010000002 DATDMP010000000
VERSION     DATDMP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555266"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /gene="glpD"
                     /locus_tag="VI195_00015"
     CDS             complement(<1..1099)
                     /gene="glpD"
                     /locus_tag="VI195_00015"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY6822793.1"
                     /translation="MSAPGGEDCDLLVVGGGINGTGIARDAAGRGLSVLLCEQHDLAA
                     HTSSASTKLIHGGLRYLEGLHFLLVRKALAEREILLAAAPHIMRPLRFVMPHAPHLRP
                     AWLIRAGLFIYDHLAHRERLAASASIDLRTHIAGAALNSGYRRGFVYSDGQADDARLV
                     VLNALDAHQRGARILTRTRCERLRAAGGRWSASLVRGGERATIHARAAVNATGPWAEQ
                     FVRAATPLEPAHQVRLVKGSHVVVPRLFEHRFAYIFQNEDRRIVFAIPYQHDFTLLGT
                     TDVDYRGDPAAVRIDAGEVSYLCALANRYFNRPISAADVRWSYSGVRPLLAEEVADPM
                     SVTRDYALELQRAPAPLLSVFGGKLTTYRKLA"
     gene            complement(1096..2019)
                     /gene="lpxL"
                     /locus_tag="VI195_00020"
     CDS             complement(1096..2019)
                     /gene="lpxL"
                     /locus_tag="VI195_00020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HEY6822794.1"
                     /translation="MQLRLLAPRYWLTWTGLGMMRLLALLPFALLVPLGRSLGAVLRH
                     LPIRFVATARRNIELCLPELTPAARERLLDRHFASLGIALLEIPFAWWGRPERIARIV
                     RIEGREHLAAARARGRGVILLTAHFTTMEMAGRVLASVTPVGFLYRPTRNEALAYALE
                     HFRRDFGGYPISRDDIRAFIAALKNNQCVWYAPDQSYRRKGAEMVPLFGIPAATNTLT
                     SRLARITGAAVLPYFLERLPGTAGYRAVIHPPLADFPSDNPAADAERFNRLIEAQVRL
                     VPEQYLWIHRRFKGLSGDYPDYYGQQAPRRA"
     gene            2040..>2637
                     /locus_tag="VI195_00025"
     CDS             2040..>2637
                     /locus_tag="VI195_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6822795.1"
                     /translation="MNVLVSAENTPFPQSICAPMPPMSRADPRYLEVRLVRANLRRGR
                     HHVLRAVSWVVRPGERWVLAGENGAGKTQLLKLLAGTVWPAPAARAVRCYRRGRHTWV
                     APEAVRDEIAYLGAERQDQYQRYGWNTSVERIVGTGVHHSDIPLDALTRSDRARVRRA
                     LGALAIAHLARRPFLRLSYGERRVVLLARVLASRPRLLL"
BASE COUNT          391 a          881 c         1007 g          358 t
ORIGIN      
        1 ccgcgagctt gcggtaggtc gtgagcttgc cgccgaacac cgagagcagc ggcgccggcg
       61 cacgctggag ctcgagggcg tagtcgcggg tgacgctcat cgggtcggcc acctcctcgg
      121 cgagcagcgg ccgtacgccg gagtaactcc agcggacgtc cgccgcggag atcggccggt
      181 tgaagtagcg gttggcgagc gcgcacaggt aggagacctc gccggcgtcg atgcgcacgg
      241 cggccggatc gccgcggtag tcgacgtcgg tggtgccgag cagcgtgaag tcgtgctggt
      301 acgggatggc gaacacgatg cgccggtcct cgttctgaaa gatgtaggcg aagcgatgct
      361 cgaacagccg cggcaccacc acgtggctgc ccttcacgag ccgcacctgg tgcgcgggct
      421 cgagcggggt ggcggcgcgc acgaactgct cggcccaggg gccggtggcg ttcaccgcgg
      481 cgcgggcgtg gatcgtcgcg cgctcgccgc cgcgcaccag gctcgcgctc cagcggccgc
      541 cggcggcgcg cagccgctcg cagcgcgtgc gcgtcaggat ccgcgcgccg cgctggtgcg
      601 cgtcgagcgc attcagcacc accagccggg cgtcatcggc ctgcccgtcg gaatacacga
      661 agccgcgccg gtagccgctg ttgagcgctg cgcccgcgat gtgggtacgc aggtcgatgc
      721 ttgccgaggc cgcgaggcgc tcgcggtgcg ccagatgatc gtagataaag agaccggcgc
      781 ggatcagcca ggccgggcgc aggtgcgggg cgtgcggcat gacgaagcgc aacggccgca
      841 tgatatgcgg cgcagcggcg agcaggatct cgcgctcggc gagcgccttg cgcaccagca
      901 ggaagtgcaa cccctcgagg tagcgcagcc cgccgtggat cagcttggtg ctcgccgagg
      961 aggtgtgcgc ggcgaggtcg tgctgctcgc acagcagcac gctcaggccg cgcccggcgg
     1021 catcgcgcgc gatgccggtg ccgttgattc cgccgccgac caccagcagg tcgcagtcct
     1081 cgccgcccgg tgcgctcatg cgcggcgcgg tgcctgctgg ccgtaatagt cgggatagtc
     1141 cccgctgagt cccttgaagc gccggtggat ccacaggtac tgctcgggca cgaggcgcac
     1201 ctgggcttcg atcaggcggt tgaagcgctc ggcgtcggcc gccgggttgt cgctcggaaa
     1261 gtcggcgagc ggcggatgga tcacggcacg gtaacccgcg gtgcccggca gacgctcgag
     1321 gaagtaaggc aacaccgccg cgccggtgat gcgtgccagg cgcgaggtga gcgtgttggt
     1381 ggcggcgggg atgccgaaca gcggcaccat ctccgcgccc ttcctgcggt agctctggtc
     1441 gggcgcatac cacacgcact ggttgttctt cagcgcggcg atgaaggcgc ggatgtcgtc
     1501 gcgggagatg ggatagccgc cgaagtcgcg gcgaaaatgc tcgagagcgt aggccagcgc
     1561 ctcgttgcgg gtggggcggt agagaaagcc caccggggtg accgaggcga gcacgcggcc
     1621 ggccatctcc atggtggtga agtgcgcggt gaggaggatc acgccgcggc cgcgcgccct
     1681 ggcggccgcg agatgctcgc ggccctcgat gcgcacgatg cgggcgatgc gctcgggtct
     1741 gccccaccag gcgaacggga tctcgagcaa ggcgatgccg aggctggcga agtgccgatc
     1801 gagcagccgc tcgcgcgcgg ccggagtgag ctccggcagg cacagctcga tgttgcgccg
     1861 ggcggtcgcg acgaagcgga tcggcagatg gcgcagcacc gcgccgagcg agcgcccgag
     1921 cggcaccagc agcgcaaagg gcagcagcgc gagcagccgc atcatgccga gcccggtcca
     1981 ggtgagccag taacgtggcg cgagcagccg cagttgcact cgggaaaacc ccgcaaggtt
     2041 tgaacgtcct ggtgagcgcg gagaatactc cattcccgca gtcgatctgt gctccaatgc
     2101 cgccgatgag ccgcgccgat ccccgctacc tcgaggtgcg cctggtgcgc gccaacctgc
     2161 gccggggccg gcatcacgtg ctgcgcgcgg tgagctgggt cgtccgcccg ggcgagcgct
     2221 gggtgctcgc cggcgagaac ggcgccggca agacgcagct gctgaaactc ctcgccggca
     2281 cggtgtggcc ggcgccggcg gcgcgcgcgg tgcgctgcta ccggcggggc aggcacacgt
     2341 gggtggctcc cgaggcggtc agggacgaga tcgcctatct cggcgcggag cgccaggacc
     2401 agtatcagcg ctatggctgg aataccagcg tcgagcggat cgtcggcacc ggagttcacc
     2461 acagcgacat tccgctcgat gcgctgacgc gcagcgaccg ggcgcgcgtg cgccgggctc
     2521 tcggcgcgct cgcgatcgcg catctcgcgc gccgtccgtt cctgcgcctg tcctacgggg
     2581 aacggcgcgt ggtgctcctc gcgcgcgtgc tcgcttcacg cccgcgcctg ctgctgc
//
LOCUS       DATDMP010000003         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_444524, whole genome shotgun sequence.
ACCESSION   DATDMP010000003 DATDMP010000000
VERSION     DATDMP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444524"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VI195_00030"
     CDS             <1..382
                     /locus_tag="VI195_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6822796.1"
                     /translation="DPLHARRALREGARGFLSKRSAPEALLEAITAVAAGQRYLDVSL
                     AQKLALGEVEHAGKSGVEQLSEREFEVFVRLAGGASVQRIAEDLKLSASTVGTHLYNI
                     KQKLGVSNQAELTLIAIRLGLIEA"
     gene            complement(427..684)
                     /locus_tag="VI195_00035"
     CDS             complement(427..684)
                     /locus_tag="VI195_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822797.1"
                     /translation="MAIRFRRRQEAPNEVIGTYSFTNRTVQVLRTQDDRILWVCDCEK
                     FRRQNGHREPLWCKHIAKAAARRSLERLTRRVTVSRELAEH"
     gene            complement(798..1406)
                     /locus_tag="VI195_00040"
     CDS             complement(798..1406)
                     /locus_tag="VI195_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822798.1"
                     /translation="MNDTPAMDEAGLAQPLAQLRTVLIPGETVEAYAIQMRLFALLHR
                     RQLIAASSGRLIVLTRKLLGGFDVTTIRWQDLEEVTLHVGMVSADLAVRAGKATDLAS
                     LGASGAQQVQFRGLRRDQAQAVYRICQSQDQAWREKRRVRELEELRARSGGIQVGSAP
                     AALAANGTSDAVRRLQEAKQMLDARLITDAEYEAIKAKIIAS"
     gene            complement(1440..>2144)
                     /locus_tag="VI195_00045"
     CDS             complement(1440..>2144)
                     /locus_tag="VI195_00045"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEY6822799.1"
                     /translation="RAHPAAEAPPMHAAAPQPATNAAPSIAHPIPGEGGASAALPALN
                     DSDPVVRDSLVGVFGRAPTEQFLVPQNIVRHIVVTVDNLSRRKVAVELRPVKPTSGQT
                     VVATHADMSTLGEANFARYAPLAKVVQGTDVKALAVVYERLYPLFQQAYEDLGYPGKY
                     FNDRLVEVIDQLLQTPEVPAPIALLQPKVFWEYADPNLENRSAGQKLLIRMGPQNARI
                     IKTKLRELRAEIVHKQ"
BASE COUNT          316 a          720 c          737 g          371 t
ORIGIN      
        1 cgacccgctg cacgcgcggc gcgccctgcg tgaaggcgcg cgcggcttcc tctccaagcg
       61 cagcgcgccc gaagccctgc tcgaggccat caccgcggtg gcggcggggc agcgctatct
      121 cgatgtcagc ctggcgcaga agctggcgct cggggaagtg gagcatgccg gcaagtccgg
      181 cgtcgagcag ttgtcggagc gcgagttcga ggttttcgtg cgcctcgccg gcggagcgag
      241 cgtgcagcgc atcgccgagg acttgaagct ttcggcctcc acggtcggca cgcacctgta
      301 caacatcaaa cagaagctcg gtgtctcgaa ccaggccgag ctgaccctga tcgccatccg
      361 gctgggtctg atcgaggcgt gaggccgtag tcagcgccgc ggtggcggct ttgcccgcag
      421 ctcgtctcag tgctccgcga gctcgcggct cacggtcacg cggcgcgtga gccgctcgag
      481 tgagcggcgc gcggcggcct tggcgatgtg tttgcaccac agcggctcac gatggccgtt
      541 ctggcggcgg aacttctcgc agtcgcacac ccacaggatg cggtcatcct gcgtgcgcag
      601 cacctgcacc gtccggttgg tgaaactgta ggtccctatg acttcgttcg gcgcttcctg
      661 gcgccgccgg aagcggatgg ccatgcagaa agcgtagcgt agcccggctg tggattgctt
      721 gtggattact tgtgcacagc gtcaccggcg cgcacgccag ccgcgcaccg ccgcacgcca
      781 gagcgccgcc cacggcttca ggaggcgatg atctttgcct tgatcgcctc gtactccgca
      841 tcggtgatca ggcgcgcatc gagcatctgc ttcgcctcct gcaggcggcg caccgcatcg
      901 ctcgtcccgt tggcagcgag cgcggccggc gcggagccca cctggattcc gcccgagcgg
      961 gcgcgcaact cttccagctc gcgtacgcgg cgcttttcgc gccaggcctg atcctgactc
     1021 tgacagatgc gatacaccgc ctgcgcctgg tcgcgccgca agccgcggaa ctgcacctgt
     1081 tgcgcgccgc tcgccccgag ggaggcgagg tcggtggcct tgccggcacg caccgccagg
     1141 tcggcactca ccatgccgac gtgcagcgtg acctcctcga gatcctgcca gcggatggta
     1201 gtgacgtcga agccgccgag aagcttgcgc gtgagcacga tgaggcggcc gctgctcgcg
     1261 gcaatcagct gcctgcggtg cagcaacgcg aacaggcgca tctggatggc gtaggcctcg
     1321 acggtctcac cgggaatcaa caccgtgcgc agctgcgcga gcggctgcgc gagtcccgcc
     1381 tcatccattg ccggtgtgtc gttcatgtcg gtggctcgct ggcggcgcgc tgcagccggt
     1441 cactgcttgt ggacgatctc ggcgcggagc tcgcgcagct tcgtcttgat gatgcgcgcg
     1501 ttctgtggac ccatgcggat cagcagcttc tgtcccgccg aacggttctc gagattcgga
     1561 tcggcgtact cccagaagac cttcggttgc agcagtgcga tcggagcggg cacctcgggc
     1621 gtctgcagca gctgatcgat gacttccacc agccggtcgt tgaaatattt tccagggtag
     1681 ccgagatcct cgtacgcctg ttggaacagc gggtacagcc gctcgtacac cacggcgagc
     1741 gccttgacat cggtgccctg caccaccttg gccagcggcg catagcgcgc gaagttcgcc
     1801 tcgccgagcg tgctcatatc cgcatgcgtt gcgaccacgg tctgtccgga ggtcggcttc
     1861 accgggcgca gctccacggc caccttgcgc cgcgacaggt tgtcgaccgt caccacgatg
     1921 tggcgcacga tgttctgcgg cacgaggaac tgctcggtgg gcgcgcggcc gaacacgccg
     1981 acgagtgaat cgcgtaccac cggatcgctg tcgttgagtg ccggcagcgc cgcggaggca
     2041 ccgccttctc ccggaatggg atgcgcgatg gagggcgcag cgttggtggc cggctgtggc
     2101 gcggcggcat gcatgggcgg ggcttcggct gcggggtgcg cgcg
//
LOCUS       DATDMP010000004         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2111162, whole genome shotgun sequence.
ACCESSION   DATDMP010000004 DATDMP010000000
VERSION     DATDMP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111162"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1404
                     /gene="apbC"
                     /locus_tag="VI195_00050"
     CDS             307..1404
                     /gene="apbC"
                     /locus_tag="VI195_00050"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEY6822800.1"
                     /translation="MTPDETLSAVRAALEAYVDPYLGESLGAAQAVRDVRASGAGFSA
                     QVVLGFPTGGYQPELTAALDQALAAAGIAAPLTLEVQSDIRAHAVQRNLKPLEDIRNV
                     VAVASGKGGVGKSTVAVNLALAWAAQGARVGILDADIYGPSQPLMLGLVGQRPTSADG
                     KHLRPLMSHGVSAMSIGFLVDAEQPMVWRGPMVTQALTQLLGETQWGALDYLVVDMPP
                     GTGDIQLTLAQRVPVAGAVIVTTPQDIALADARKGLKMFEKVSVPVLGVVENMSVHIC
                     SNCGHSEHIFGAGGGERMAREYGVRLLGELPLDAHIREEADGGRPTVIAAPATARARP
                     YFEMARRTAAALALRARDRSALFPKIVVEET"
     gene            complement(1437..>2351)
                     /locus_tag="VI195_00055"
     CDS             complement(1437..>2351)
                     /locus_tag="VI195_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEY6822801.1"
                     /translation="RSAGKIKRARRAWGVARVHGRCGVIAPEDLGAYAHTPTRTGSRG
                     AMYFLTSRPGWVLILLLFVFTAFAMVGPFIVRQRIRLAHLRGNNEVAGFKFAVIGVLY
                     AVLLAFVVIISWERFYDAERALAREAGAAATVFRLCGGLDETTAATIRTNLAAYLRSV
                     LADDWPAMARGRSSAATTRMLSDLYEVLVHYRPADLHDSDLQKDLLTELDDLTRARRE
                     RLVMGEGTVPKVIWFVIFLGAALTISFTFFFGTENLIAQSLMTGTLAAIILSAVVVVI
                     ALDRPYTGAITVSKEPIRAVLEDFRTAP"
BASE COUNT          393 a          774 c          787 g          397 t
ORIGIN      
        1 tcagccgcgc gacggctttt tgcgtgtcgg agctggtcgg gtcgggcgag gaccggatct
       61 cggtgatcag ccggtcggca cgcaagttcg tgaaaaagct cattcagcct ttccgtcgca
      121 gcagctccct gcacacatcc agccggggat gatgccctat catctactat aaggagcaag
      181 cgcaccggag ttctgtgaat tatgccatag ccttgagccc ggccagggcc cgctttgtgc
      241 gctccatctc gctgccctct acaattcagc actatcgtcc tccgggctta tctcacggtt
      301 ttcaacatga cccccgatga gaccctgtct gcagtgcgcg ccgcgctgga ggcgtacgtc
      361 gacccctatc tcggcgagtc gctcggcgcc gctcaggccg tccgggacgt gcgggcatcg
      421 ggtgccgggt tcagcgcgca agtggtgctc gggttcccga ccggcggtta ccagccggag
      481 ctcactgctg cgctcgacca agctctcgcc gcggccggga ttgcggcgcc actcaccctc
      541 gaggtgcagt ccgacattcg cgcgcacgcg gtacagcgca atctcaagcc gctcgaggac
      601 atccgcaacg tcgtggcggt cgcttcaggc aagggcggcg tgggcaagtc taccgtcgcg
      661 gtgaatctcg cactcgcctg ggctgcgcag ggcgcccgtg tcgggattct cgacgccgat
      721 atctacggcc caagtcagcc gctcatgctg ggcctggtcg ggcagcgccc cacatcggcc
      781 gatggcaagc acctgcggcc gctcatgagc cacggcgtca gtgccatgtc gatcggtttc
      841 ctcgtcgatg ccgagcagcc gatggtgtgg cgcggtccca tggtgacgca ggcgctcacg
      901 cagctgctcg gtgagacgca gtggggtgcg ctcgattacc tggtggtcga catgcctccc
      961 ggcaccggcg acatccagct gacactcgcg cagcgcgtgc cggtcgccgg ggccgtcatc
     1021 gtgaccacgc cgcaggacat tgcgctggca gatgcgcgca agggcctcaa gatgttcgag
     1081 aaggtgtcgg tgccggtgct cggcgtcgtc gagaacatga gcgtgcacat ctgctcgaac
     1141 tgcggccaca gcgagcacat attcggtgcg ggtggcggtg agcgcatggc gcgcgaatat
     1201 ggtgtgcggc tgctcgggga gctgccgctc gatgcgcaca tccgtgagga ggccgatggc
     1261 gggcgtccga cggtgatcgc agcgcccgcg acagcacgcg cccggccgta cttcgagatg
     1321 gcgcggcgca cggcggcagc gctcgccctg cgcgcccgcg accgcagcgc gctgtttccg
     1381 aagatcgtgg tcgaggaaac ctgagcgggc cccgtgcgca gcgctgcgcc cgcccgctac
     1441 ggagcggtgc ggaagtcctc gagcaccgcg cggatgggct ccttggagac cgtgatcgcg
     1501 cccgtgtaag gccgatcgag agcgatgacc acgacgacgg ccgagaggat gatcgccgcg
     1561 agtgtgccgg tcatcaatga ctgcgcgatc aggttctcgg taccgaagaa gaaggtgaag
     1621 gagatcgtga gcgcggcgcc caggaaaatg acgaaccaga tcaccttcgg gaccgtgccc
     1681 tcgcccatca ccagccgctc ccggcgtgcg cgggtcaggt catcgagctc ggtcaacaag
     1741 tccttctgaa gatccgagtc gtgcaggtcg gcggggcggt agtgcacgag cacctcgtac
     1801 aagtcgctca gcattcgcgt ggtggcagcg ctcgagcgcc cgcgggccat ggccggccag
     1861 tcatccgcca gcactgatcg caggtacgcc gccaggttgg tgcgtatcgt ggccgcggta
     1921 gtctcatcca gaccgccgca caggcggaag acggtagccg cagctcccgc ttcccgcgcc
     1981 agcgccctct cggcgtcgta gaagcgttcc caggagatga tcacgacgaa agcgagcagc
     2041 accgcgtaca gcacgccgat caccgcgaac ttgaagccgg ccacttcgtt gttgccgcgc
     2101 aggtgtgcga ggcggatacg ctggcgcacg atgaaggggc cgaccatggc gaatgccgtg
     2161 aacacgaaca gcagcaggat cagcacccac cctggccgac tggtgaggaa gtacatggcg
     2221 ccccggctcc cggttcttgt tggagtgtga gcgtaggcgc caagatcctc gggcgcaatc
     2281 acgccgcacc ggccgtggac ccttgctacg ccccaggctc tccttgcgcg ctttattttt
     2341 cctgcagatc t
//
LOCUS       DATDMP010000005         4759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1666802, whole genome shotgun sequence.
ACCESSION   DATDMP010000005 DATDMP010000000
VERSION     DATDMP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666802"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VI195_00060"
     CDS             <1..479
                     /locus_tag="VI195_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6822802.1"
                     /translation="KTYPILILTARDNWQDKVEGLQAGADDYVVKPFHFEEVLARLQA
                     LLRRAGGWASPQLKCGPIVLDTRAQTVTVDGVAVELTTFEYRILEHLMLRAGEVISKS
                     ELTERLYDQDFERDSNVIEVLVGRLRRKLDPEDRLRPIETLRGRGYRMGLPREAPI"
     gene            498..1826
                     /locus_tag="VI195_00065"
     CDS             498..1826
                     /locus_tag="VI195_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6822803.1"
                     /translation="MHSLSRRLLISVSVPLALFCGVVMLVLDTGFREVAQRTLQDELD
                     RQMVALIAAAEPQPDGGYAPAAHSLDPRLDTPRSGLYAEIRTAAHDWRSTSTAGLNTD
                     FGRLLSGGERKLSYASFGDDRVAVASRSIQFEGEPAEARSLTFSVATSLSPYQQELWR
                     FRRKLGGWFVALMLLLLVTLAALLHWVLGPVRRMEREIHEVEEGRRERLGVGYPRELA
                     GVAGNLNALLIGERKRVARYRDTLGNLAHSLKTPLAVMRAEGANAMVSTEIDRMSGII
                     EHQLKRAAASGGALLGQAPVEVAQVAAELRATLLRVYGRKDLAIELAVAPAAQFIGDR
                     GDLTELLGNLLDNACKWCRTRVRFSATTDDSVESRERLGLVVEDDGAGISAANRARVL
                     QRGVRADEKVPGHGLGLAMVHDTVDLYGGKLSIDASPLGGARFTVRLPGR"
     gene            complement(1908..3446)
                     /locus_tag="VI195_00070"
     CDS             complement(1908..3446)
                     /locus_tag="VI195_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6822804.1"
                     /translation="MNTNTDAVLRRLGLGPMNPGAWSGSRGWSSASGNALVNVRNPAN
                     GTLIAQVRPASAEDYEHVVSSAIEVAARWREVPAPKRGETVRLIGDELRRHKEDLGTL
                     VSLENGKILAEGLGEVQEMIDIADFAVGQSRMLYGLSMHSERPQHRMFEQWHPLGVIG
                     IISAFNFPVAVWSWNAFLGAVCGNACVWKPSHKTPLTALAVQHLCNRVLESAHLPAVF
                     QVFVDGGTGLASRLVEDRRIALISFTGSTEVGRKVGERVAARLGKSLLELGGNNAVIV
                     DETANLDLAVPAIVFGAVGTAGQRCTSTRRVFAHKAIAAELERRLVHAYAQVRIGDPL
                     ESGTLMGPLIDSQAVESYQAAVSAARDAGGELLHGGSVLDRPGNFVEPAIIRARNEWS
                     IVQTETFAPILYVIPVDSLNEAIAQQNASAHGLSSALFTDRLQNAETFLASSGSDCGI
                     ANINLGTSGAEIGGAFGGEKDTGGGRESGSDAWKTYMRRQTNTVNWSRDLPLAQGIEF
                     KVES"
     gene            complement(3443..4615)
                     /locus_tag="VI195_00075"
     CDS             complement(3443..4615)
                     /locus_tag="VI195_00075"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6822805.1"
                     /translation="MHRVLILGAGKIGALISGLLAESGAYRVQLADLDGAAAEAVVRA
                     HGSGHLAALTLDASNASALVQQLAAHPVDAVISSLPYHCNVRVAEAARKARLHYFDLT
                     EDVAVTRAVRTIAAGAEQAFVPQCGLAPGFISIAAAELITHFDTLRAVKLRVGALPQH
                     PNNVLKYSLTWSTEGLINEYGNPCQAVSEGRLVELAPLEGLEEIEIDGMRYEAFNTSG
                     GLGSLAETHGGRVEAMNYKTIRYPGHCEQMRLLMNDLKLNHDRATLKRILENAVPQTL
                     QDVVIVYVAVTGTQDGQLREENYVNKIYPQMIAGRLWSAIQVTTAAGITAVVDLVLES
                     GHRYSGFVAQEQFRLTDILANRFGRHYAPGGTKHVSGEVVASGKVGHQRRAKAVNS"
BASE COUNT          776 a         1572 c         1617 g          794 t
ORIGIN      
        1 gcaaaaccta tccgatcctg atcctgacag cccgcgacaa ctggcaggac aaggtggaag
       61 ggctgcaggc gggcgcggac gactacgtgg tcaaaccgtt ccacttcgag gaggtgctgg
      121 cgcggttgca ggcgctgctg cgccgcgctg gtggttgggc cagtccgcag ctcaagtgcg
      181 gacccatcgt cctcgacacg cgcgcccaga cggtcacagt ggatggcgtt gcggtggagc
      241 tcaccacctt cgaataccgc attctcgaac atctgatgct gcgcgccggc gaggtgatct
      301 ccaagagcga gctgaccgag cggctctatg accaggattt cgaacgcgac agcaacgtca
      361 tcgaggtgct ggtcggacgg ctgcgccgca aactcgatcc cgaggaccgc ttgcggccga
      421 tcgagacgct gcgcggacgc ggatatcgta tggggctgcc gcgggaggcg ccgatctgac
      481 acggcggtgg aggccgcgtg cactcactca gccggcgact gctcatctcg gtgtcggtgc
      541 cactcgcgct gttttgcggc gtggtgatgc tggtgctcga taccggcttc cgcgaggtcg
      601 cgcagcgcac actgcaggat gagctcgacc gccagatggt ggcgctcatc gcagccgccg
      661 agccgcagcc cgatggcggc tacgcgcccg ctgcgcacag cctcgatccg cgcctcgaca
      721 cgccgcgttc gggcctgtat gcagagatac gcaccgccgc gcatgactgg cgctcgacat
      781 cgaccgcggg gctgaatacc gatttcggcc gcctgctctc cggcggagag cgcaagctgt
      841 cctatgccag tttcggcgac gaccgcgtgg ccgttgccag ccgctcgatt cagttcgaag
      901 gcgagcccgc ggaggcgcgc tccctcacct tcagcgtggc gacgagtctc agtccctatc
      961 agcaggagct gtggcgcttc cggcgcaagc tcggcggctg gttcgtcgcg ctgatgctgc
     1021 tgctgctcgt gaccctcgcc gcgctgctgc actgggtact cgggccggtg cggcgcatgg
     1081 agcgtgagat ccacgaggtc gaggagggcc gacgcgagcg actcggcgtc ggctacccac
     1141 gggagctcgc cggcgtcgcg ggcaacctca atgccctgct gatcggcgag cgcaaacgcg
     1201 tggcgcgcta ccgtgacaca ctcggtaatc tcgcccatag cctgaagact ccgctcgccg
     1261 tgatgcgcgc ggaaggcgcg aacgccatgg tgagcaccga gatcgaccgc atgagtggca
     1321 tcatcgagca tcagctcaag cgcgccgccg cctccggcgg ggcgctgctc ggacaggcgc
     1381 cggtggaagt cgcacaagtg gccgccgagc tgcgcgccac tctgctgcgg gtctatggcc
     1441 gcaaggatct cgccatcgag ctcgcggtgg caccggcggc gcaattcatc ggcgatcgcg
     1501 gcgatctcac cgagttgctc ggcaacctgc tggacaacgc ctgcaagtgg tgccgcaccc
     1561 gcgtgcgctt cagtgcgacg accgatgaca gcgtcgaatc ccgtgagcgc ttgggtctcg
     1621 tggtggaaga cgacggcgcg gggatctcgg cggccaatcg cgcacgcgtg ctccagcgcg
     1681 gggtgcgagc ggatgagaag gtgccgggcc acgggcttgg gctcgccatg gtgcacgaca
     1741 ccgtggacct ctatggcggc aaactctcca tcgatgcctc gccgctcggt ggcgcgcggt
     1801 tcacggtacg cttgccaggg cgctgagccc ggaggctgca tgcacgttcg ggcaatggtt
     1861 taccaaatgg ttaaccattg ctcaaaacac cctgcaatgt ctccgcccta cgattcgacc
     1921 ttgaactcga tcccctgcgc gagcggcaga tcgcggctcc agttcaccgt gttggtctgg
     1981 cggcgcatgt aggtcttcca ggcatccgaa cccgactcgc ggccgccgcc ggtgtctttc
     2041 tcgccaccga aggcgccgcc gatctcggcc ccggaggtgc cgagattgat gttggcgatg
     2101 ccgcagtcgc tgcccgagga cgcgaggaag gtttccgcgt tctgcaggcg atcggtgaac
     2161 agcgccgagg acagtccgtg agccgaggcg ttctgctgcg cgatcgcctc attcagcgag
     2221 tcgaccggga tcacgtaaag gatcggagcg aaggtctcgg tctggacgat ggaccactcg
     2281 ttgcgcgcgc ggatgatggc cggctcgaca aagttgccgg gacggtcgag gacgctgccc
     2341 ccatggagca gctctccgcc ggcgtcgcgc gctgcgctca ccgcggcctg atagctctcg
     2401 accgcctggg agtcgatgag cggtcccatg agcgtaccgc tctcgagcgg atcgccgatg
     2461 cgtacctggg cgtaggcgtg caccaggcgg cgctcgagct ccgcagcgat cgccttgtgg
     2521 gcaaagacgc gacgggtcga ggtgcagcgc tggccggcgg tgcccacggc tccgaacacg
     2581 atggccggga cggcgagatc gagattggcg gtctcatcca cgatcaccgc gttgttgccc
     2641 ccgagctcga gcaggctctt gccgagccgc gccgcgacgc gctccccgac cttgcggccg
     2701 acctcggtgg agcccgtgaa ggaaatgagt gcgatgcgcc ggtcctcgac cagacggctg
     2761 gcaagcccgg tgccgccatc gacgaagacc tgaaacaccg caggcagatg ggcggactcg
     2821 agaacgcggt tgcacaggtg ctgcacggcg agggccgtga gcggtgtctt gtgcgagggc
     2881 ttccacacgc aggcgttgcc gcagaccgcg ccgaggaatg cgttccacga ccacaccgcg
     2941 accggaaagt tgaacgcgga gatgatgccg atgaccccga gcggatgcca ttgctcgaac
     3001 atgcggtgct gcggacgctc cgagtgcatg ctgagtccgt acaacatgcg cgactgaccg
     3061 accgcgaagt ctgcgatgtc gatcatttcc tgcacctcac cgagcccttc ggccaggatc
     3121 ttgccgttct ccagcgagac cagcgtgccg aggtcctcct tgtggcggcg cagctcatcg
     3181 ccgatgagcc tcacggtctc accacgcttg ggagcaggca cctcacgcca gcgcgcagcg
     3241 acctcgatcg ccgagctcac cacgtgctcg tagtcctcgg cgctcgccgg tcgtacctgg
     3301 gcgatgagcg tcccgttggc cggattgcga acgttgacaa gcgcgttgcc gctggcgctc
     3361 gaccagccac gcgatcctga ccatgcgccc ggattcatcg gcccgaggcc cagacgacgc
     3421 agcactgcat cggtattggt gttcatgagt tcacagcctt tgcgcggcgt tgatggccca
     3481 ccttgcccga ggccacgacc tccccggaga cgtgcttggt gccgccgggg gcgtagtggc
     3541 gtccgaaacg gttggcgagg atgtcggtga ggcggaactg ctcctgcgcc acgaagccgc
     3601 tgtaacggtg gccgctttcg agcacgaggt ccacgaccgc ggtgattccg gccgcggtcg
     3661 tcacctggat cgccgaccac agccggccgg cgatcatctg agggtagatc ttgttgacgt
     3721 agttttcctc gcgcagctgt ccgtcctgcg tgccggtgac cgcgacgtac acgattacca
     3781 cgtcctgcag cgtctgcggc accgcgttct ccaggatgcg cttgagcgtg gcgcgatcat
     3841 gattgagctt cagatcgttc atgagcaggc gcatctgctc gcaatgaccg gggtaacgga
     3901 tggtcttgta gttcatcgcc tcgacgcggc caccgtgcgt ctccgcgagc gacccaagtc
     3961 ctcccgaggt attgaaggcc tcgtagcgca tgccgtcgat ttcgatctct tcgagtccct
     4021 cgagcggcgc gagctcgacc agccttccct cgctgaccgc ctggcagggg ttgccgtact
     4081 cgttgatgag cccctcggtc gaccaggtga gggagtactt cagcacgttg ttcggatgct
     4141 gcggcagggc cccgacacgc agcttgaccg cgcgcagcgt atcgaagtgg gtgatgagct
     4201 cggcggcggc gatgctgata aacccggggg caagcccgca ctgtggcacg aaggcctgct
     4261 cggcgcctgc ggcgatggtg cgcaccgccc gcgtcactgc cacatcctcg gtgagatcga
     4321 agtagtgcag ccgcgccttg cgagccgcct cggcgacgcg cacgttgcag tggtagggga
     4381 ggctcgagat caccgcatcc accgggtgcg cggcgagctg ctgcacgagc gcgctggcgt
     4441 tgctggcatc gagggtgagt gcggcgaggt ggccgctgcc gtgcgcgcgc accaccgcct
     4501 cggcagccgc gccgtcgagg tctgcgagct gcacccggta agcgccggac tcggccagca
     4561 atcccgagat cagtgcgccg atcttgccgg cgccgagaat caagacacgg tgcataggac
     4621 cctcgttaaa aggggcgcat tctgcctttg aagcgccgcc ggcgccagcc acccgcgagc
     4681 cggcgcggaa cgccgccggg cacggcctga ggggcgacga acacatggta ggatcaatga
     4741 catgcgagcg cccgcaacc
//
LOCUS       DATDMP010000006         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1333617, whole genome shotgun sequence.
ACCESSION   DATDMP010000006 DATDMP010000000
VERSION     DATDMP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333617"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1538)
                     /locus_tag="VI195_00080"
     CDS             complement(219..1538)
                     /locus_tag="VI195_00080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY6822806.1"
                     /translation="MTTVTMPQLGESVTEGTILKWLKQPGDAVALDDSLCEIETEKVT
                     AELPSPFEGTMGEILVPEGETVDVGTPLCSVEESATAPSTNGAVHAPGPKQTSGAWSG
                     GPMAIHDEPGPGPSEAGPAPPASTARVPVAAAPRRPEARERFYSPAVMRLANEHRIDL
                     SMLTGSGVGGRVTRKDVEAVIASPPAAAATAPDAVAPPPPRRAAPGVAPYETVQLSPT
                     RRTIAENLRRSNLEAPQAWTMVEADVTGLVALRAGEKERFRRDEGVELTLLPYFTEAV
                     CQALREFPMLNARWEGDELRRYHTLEIGIAVASESGLVVPVIHDAGDLSVTGLAKRIA
                     DLAQRAHARKLRVEDIEGGTFTVNNTGAFGSLSSKPIVNYPQVAIVTMERVVKRPVVL
                     ENDAIAVRSMMNVCLSFDHRAMDGLEAGGFLGALKQRLEAISPAGHR"
     gene            complement(1547..>2517)
                     /locus_tag="VI195_00085"
     CDS             complement(1547..>2517)
                     /locus_tag="VI195_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6822807.1"
                     /translation="SIEAFYAHMPGLKVVAPAFPADYRSLLKASIADPNPVIFLEHKR
                     TYRLIDSPLAESSIIGISIGAAHYGMRPVAEIQFADFIHSGFDQLCSEASRWCYRTNG
                     DASLPMVVRTPWGGGVHGGQHHSQSIEAFYAHMPGLKVVAPAFPADYSSLLKASIADP
                     NPVIFLEHKRTYRLIRQELDGAPAEQSIGKARISRQGSDVTIIAYGFMLHESLAAAQT
                     LAGEGIECTVVDLMTLLPLDVETILESARATGKVLVVHEDTLTGGLGGEISALIAEHA
                     FEYLDAPIRRLAGPDIPPMPFHPAMEHYFMPDRDKIAAAARELAAY"
BASE COUNT          402 a          827 c          836 g          452 t
ORIGIN      
        1 ctaccggggg ggcgtagagg acgcgcttgg caatgccctt atggcactct tcgcaggtgt
       61 gggtcgtttc cgcgtcgaag ccctggcgaa gctcgtaggt ggctttgcag cgcgggcagt
      121 gatactcgta ggttggcaaa agggacacct cgagcatcgc aacgagtacg aagctcggct
      181 gttattgttt gctgtttagc actcttgagt caagagtgct aacggtggcc cgccggcgag
      241 atcgcttcca ggcgctgttt gagcgccccc aggaagccac cggcttcgag tccatccatg
      301 gcgcgatggt cgaagctcag gcacacgttc atcatcgacc gcacggcgat ggcgtcgttt
      361 tcaagcacta ccggccgctt caccacccgc tccattgtca cgatggcgac ctgcgggtag
      421 ttcacaattg gcttcgacga gagggagccg aaggcgccgg tgttattgac ggtgaaggtg
      481 ccgccctcga tgtcctccac ccggagcttc cgcgcgtgcg cccgctgcgc gagatcggcg
      541 atgcgcttcg cgagccccgt cacgctcaga tccccggcat cgtggatcac cggcaccacc
      601 agccccgact cgctggcgac cgcgataccg atttcgagcg tgtggtagcg gcgcagctcg
      661 tcgccttccc agcgcgcgtt gagcatcgga aattcccgca gcgcctggca caccgcctct
      721 gtgaagtacg gcagcagcgt aagctccacg ccttcgtcgc ggcggaagcg ctccttttcg
      781 ccagcccgaa gtgcgacgag gcccgttacg tccgcctcca ccatcgtcca ggcctggggt
      841 gcttccaggt tcgaacggcg gaggttctcc gcgatcgtgc gcctcgtggg cgacagctgg
      901 actgtctcgt acggcgccac tccgggggcc gcacggcgcg gaggaggtgg cgcaacagcg
      961 tctggcgccg ttgcggctgc ggctggcggt gatgcaataa cggcctctac gtccttacgc
     1021 gtcacacggc caccgacgcc gctccccgtc agcatcgata agtcgatgcg gtgctcgttg
     1081 gcgaggcgca tgaccgcggg cgagtagaac cgctcacgcg cctccgggcg ccttggagcc
     1141 gccgccaccg gaactcgtgc agtgctcgcc gggggggcgg gccccgcctc cgacggaccc
     1201 ggcccgggct cgtcgtggat cgccatgggg ccaccgctcc acgcgcccga cgtttgtttg
     1261 ggcccggggg cgtgtacggc gccgttggtg gacggcgcgg tcgcggactc ttcgaccgaa
     1321 cacagcggcg tgcccacatc gaccgtctcg ccctcgggga cgaggatctc gcccatcgtc
     1381 ccctcgaacg gcgatgggag ctccgcggtc accttctcgg tctcgatctc acagagcgag
     1441 tcatcgagcg cgacagcgtc ccctggctgc ttgagccact tcaggatcgt gccttcggtg
     1501 acgctctcgc cgagctgcgg catggtgacg gtcgtcatcg tgggcctcag tatgctgcca
     1561 gctcgcgcgc ggcggcggcg atcttgtccc ggtctggcat gaagtagtgc tccattgcgg
     1621 gatggaacgg catcggcgga atatcgggcc cggcgaggcg ccggatcggt gcgtcgagat
     1681 actcgaacgc gtgctcggcg atgagcgccg agatctcgcc gccaaggcca ccggtgagcg
     1741 tgtcctcgtg cacgaccagc acctttccgg tggctcgcgc cgattcaagg atcgtctcga
     1801 cgtccagcgg tagcagcgtc atcaagtcca cgaccgtgca ctctatccct tcgccggcga
     1861 gcgtttgggc cgcggcgagc gactcatgca gcatgaagcc gtaggcgatg atcgtgacgt
     1921 cacttccctg ccgggatatg cgcgccttgc cgatcgactg ctcggccggc gcaccatcga
     1981 gctcctgccg gatgagccgg tacgtccgct tgtgctccag gaaaatcacc gggttcggat
     2041 cggcgatcga tgccttcagc aggctgctgt agtcggcggg gaacgccggc gcgacgacct
     2101 tgagcccggg catgtgggcg tagaacgcct cgatgctctg ggaatgatgc tggcccccgt
     2161 gaacaccgcc accccacggc gtccgcacca ccatcggcag ggaggcgtcg ccgttcgtgc
     2221 ggtagcacca gcgcgaagct tcgctgcaga gctggtcgaa gccgctatga atgaaatcgg
     2281 cgaactggat ttcggcgacg ggccgcatgc cgtagtgggc ggcaccaatg ctgatcccga
     2341 tgatcgacga ttccgcaagc ggcgagtcga tgagccggta cgtccgcttg tgctccaaga
     2401 aaatcaccgg gttcggatcg gcgatcgatg ccttcagcag gctgcggtag tcggcgggaa
     2461 acgccggtgc gacgaccttg agcccgggca tatgggcata aaacgcctcg atactct
//
LOCUS       DATDMP010000007         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2111324, whole genome shotgun sequence.
ACCESSION   DATDMP010000007 DATDMP010000000
VERSION     DATDMP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111324"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VI195_00090"
     CDS             <1..822
                     /locus_tag="VI195_00090"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY6822808.1"
                     /translation="KRDPRDPWERMNRASYKFNDAIDRAVVKPVARGYTHLPQGVQSG
                     VHNFFDNLQTPVVIVNDLLQGQVKSFFSDIGRLLLNSTVGLGGLFDMATAVNMEKNDR
                     DFGQTLGKWGVPKGPYLMVPIFGPYDVRDGVGAITVDYYANPRNYAPFWVNTGLWLVN
                     GVDRRARLLPLDATIQSAYDPYAFIRNAYLQNRDFKVRGDQSQHEQEQEEKLMEEAEH
                     ESEGGQPAAPQPETPKTETPQSQSQPPQAPQPQQSQPPQPQSSQPQAPQPQQPQR"
     gene            complement(819..1160)
                     /locus_tag="VI195_00095"
     CDS             complement(819..1160)
                     /locus_tag="VI195_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6822809.1"
                     /translation="MSLPEAARTTAAGAFRLVAAPSGGLAAQGPLNFASARRACELGV
                     RALAAATGAKLEIDCHGITVSDSAGLAVLLEWLGVAKRAGRALRYTQLPEGLAALAHI
                     SDVQELLERGV"
     gene            complement(1170..1880)
                     /locus_tag="VI195_00100"
     CDS             complement(1170..1880)
                     /locus_tag="VI195_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6822810.1"
                     /translation="MSMATRAALSGLALALGFAWAQAQSPQPAPGTAAAPPAGTSAAA
                     GATAVQGPSEVVQAAAQGMLNDLDKDRAAYRREPAKVGQLVDKYLLPHWDTEYSARLV
                     LGKYWRTATPEQRHRFIDAFYHSLLSNYGAALAEFTADRLKIYPSNVDPGKSIATVRT
                     EVKRDNGDRVSVNYYMHMTPQGWKAWDVVIDGISYVNSYRTDFGAQIEAQGIDAVIKR
                     LEAGEKPGAIGKQTSGKS"
     gene            complement(1877..2422)
                     /gene="mlaD"
                     /locus_tag="VI195_00105"
     CDS             complement(1877..2422)
                     /gene="mlaD"
                     /locus_tag="VI195_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY6822811.1"
                     /translation="MRANRTLEIGTGLFVLLGFAALLFLTTQLPASGLKLGGAKAGYH
                     VTAEFDDIGDLKVGSPVTMSGVRLGEVTGIRFDPKDFKAVVTMRIDRQYNQIPEDSFA
                     SIQTQGLLGGKYIGLSPGGLDNYLKDGSRIDQTQSAIVLESIINKFFANYSSGKGSDQ
                     NAGSAGPGKPTDNTGKAESHQ"
     gene            complement(2438..>2633)
                     /locus_tag="VI195_00110"
     CDS             complement(2438..>2633)
                     /locus_tag="VI195_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6822812.1"
                     /translation="DLHDVSEGMVKSVVFGVACSLIAVHEGYHAEPTAEGVGLATTRT
                     VVASSVLTLLLDYVLTAAFL"
BASE COUNT          451 a          882 c          856 g          444 t
ORIGIN      
        1 aagcgcgacc cgcgtgatcc ctgggagcgg atgaaccgtg cctcgtacaa gttcaacgat
       61 gcgatcgacc gcgccgtcgt caagccggtg gcccggggct atacccacct gccgcagggg
      121 gtgcagagcg gcgtccacaa cttcttcgac aatctgcaga cgccggtcgt catcgtgaac
      181 gacctgctgc agggccaggt gaagtcgttc ttctccgaca tcgggcgact gctgctcaac
      241 agcaccgtcg gactcggcgg actgttcgac atggcgacgg ccgtcaacat ggagaagaac
      301 gaccgggatt tcgggcagac gctcggcaaa tggggcgtgc cgaaggggcc ctacctcatg
      361 gtgccgatct tcggaccgta cgatgtgcgt gacggcgtcg gagcgatcac ggtcgactac
      421 tacgccaacc cgcgcaacta cgcccccttc tgggtcaata cgggcctgtg gctggtgaac
      481 ggagtcgacc gccgcgcgcg gctgctgccg ctcgacgcca cgattcagag tgcctacgac
      541 ccctacgcct tcatccgcaa cgcttacctg caaaaccgcg acttcaaggt gcggggcgac
      601 cagtcgcagc acgagcagga gcaggaggaa aagctgatgg aggaagccga gcatgagagc
      661 gagggcgggc agccggcggc cccgcagcca gagacgccaa agaccgaaac gccgcaatcg
      721 cagagtcaac cgccgcaggc gccgcagccc cagcagtcgc agccgccgca accgcagtca
      781 tcacagccgc aggccccaca gcctcagcag ccgcagcgct aaacaccgcg ttcgagcagc
      841 tcctgaacgt cgctgatgtg cgcgagcgcc gcgaggccct ccggtagctg cgtgtagcgc
      901 agcgcgcggc ccgcccgctt cgcgacgccg agccactcga gcagcaccgc gagccccgca
      961 ctgtcggaga cggtgatgcc gtggcagtcg atctcgagct tggcgccggt cgccgcggcg
     1021 agtgcgcgta cgccgagctc gcacgcgcgg cgcgcgctcg cgaagttgag tggcccctgc
     1081 gcagcgaggc cgcccgatgg cgccgccacc aggcggaagg caccggcagc ggtggtgcga
     1141 gcagcttctg gcaggctcat gccgctcctc tagctcttgc cggaggtctg cttgccgatc
     1201 gcgccgggtt tctcgcccgc ttcgaggcgc ttgatgactg catcgatgcc ctgcgcctcg
     1261 atctgtgcgc caaagtcggt gcggtagctg ttgacgtaac tgatgccgtc gatgaccacg
     1321 tcccacgcct tccagccctg cggcgtcatg tgcatgtagt agttgaccga gacgcgatcg
     1381 ccgttgtcgc gcttgacctc ggtgcgtacc gtcgcgatcg acttgcccgg atcgacgttg
     1441 ctcggataga tcttcagccg gtcggcggtg aactccgcga gcgcggcgcc gtagttgctg
     1501 agcagcgaat ggtagaacgc atcgatgaag cggtggcgct gctccggggt ggcggtgcgc
     1561 cagtacttgc cgagcaccag gcgcgccgaa tattccgtat cccagtgcgg cagcaggtac
     1621 ttgtcgacca gctggcctac cttggccggc tcgcgccggt aggctgcgcg gtctttgtcg
     1681 agatcgttca acatgccctg ggcggcagcc tgtaccacct ccgagggccc ctggaccgct
     1741 gtcgctcccg cggcggcgct cgtgcccgcg gggggcgcgg ccgctgtgcc cggagcgggc
     1801 tgagggctct gcgcctgtgc ccaggcgaag cccagtgcca gcgccaggcc gctcaaggcc
     1861 gcccgtgttg ccatgctcat tggtgtgact ccgccttgcc tgtgttgtcc gtgggcttgc
     1921 ccgggcccgc cgagcccgca ttctgatcgc tgcccttgcc gctcgagtag ttggcgaaga
     1981 acttgttgat gatgctctcg agcacgatcg ccgactgagt ctggtcgatg cggctgccat
     2041 ccttgaggta attgtcgaga cccccggggc tcaggccgat gtacttgccg ccgagaagtc
     2101 cctgcgtctg gatgctcgcg aagctgtcct cgggaatctg gttgtactgc cgatcgatgc
     2161 gcatggtcac tacggccttg aagtccttgg gatcgaagcg gatgcccgtc acctcaccga
     2221 gacgcacgcc cgacatggtg accggggacc caaccttgag atcgccgatg tcgtcgaact
     2281 cggcggtcac gtggtagccc gccttggcgc cgccgagctt cagtccgctc gccggcagct
     2341 gcgtggtcag aaacagcagt gcggcaaagc cgagcaacac aaacaatccg gttcctatct
     2401 cgagggttcg attggcacgc ataagccgaa gttcctccta caggaatgcc gccgtcaaca
     2461 cataatccag cagaagtgtc agcaccgatg aggcgaccac ggtgcgcgtg gtggcgaggc
     2521 cgacgccttc cgccgtgggt tcggcatgat agccctcgtg cacggcgatg agactgcagg
     2581 ccacaccaaa caccacgctc ttcaccatac cctcgctgac atcgtgcaga tcg
//
LOCUS       DATDMP010000008         3387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_111602, whole genome shotgun sequence.
ACCESSION   DATDMP010000008 DATDMP010000000
VERSION     DATDMP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111602"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..866
                     /locus_tag="VI195_00115"
     CDS             108..866
                     /locus_tag="VI195_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY6822813.1"
                     /translation="MSSLLRSAPLSLLVIVLSLPMTARLAASQDARAAGDTPTAKPSA
                     PLKIGIIGTGHIGGALATLWVGAGHEVLMASRHPQELQGLAHSLGPRAHVGTPTEAAR
                     FAEVVLISVPYGALPQVGRELKNELAGKIVLDTGNPYPERDGPMAVEARRKGTGVASA
                     EYLPGVRLVRAFNAINSGDLRSEAHRKGAPIAIPLAGDDGEALRVAEQLVKDAGFAPV
                     VVGPLAKAREFDVGTPVYTRLMTEPQLRSALGLK"
     gene            complement(879..1295)
                     /locus_tag="VI195_00120"
     CDS             complement(879..1295)
                     /locus_tag="VI195_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6822814.1"
                     /translation="MNITGGCLCRAVRYSISAPPIVARTCWCRVCQYLGAGGATVNVC
                     FPAEALDVRGELREYRAVADSGNPMQRRFCPLCGTHLFTGTTARPHLIFVRAGSLDEP
                     ELAKPAMSIWTSSAPSWACFDERIPKVERQPPPPVT"
     gene            complement(1513..2238)
                     /locus_tag="VI195_00125"
     CDS             complement(1513..2238)
                     /locus_tag="VI195_00125"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEY6822815.1"
                     /translation="MAAVPGRPLDFPDLLNARDLGGYPTTDGAETRWRSLLRADDLAQ
                     LSDLGLQALADYGVRTILDLRWPEEAAQAPSPVPDRLPQLRYARISLLSHTEDDWRLR
                     SRDAAKELWKCVVLEHVRAELRTVLGFIAAAPREPLLFHCVAGKDRTGLIAALLLALA
                     DATPAAIAQDYSVSAEYLREGYLRRYPHTERERILEALRCPEQGAYNMLAFLERAGGV
                     RAYLAKIGLSADEIARLRARLRD"
     gene            complement(2253..3209)
                     /locus_tag="VI195_00130"
     CDS             complement(2253..3209)
                     /locus_tag="VI195_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6822816.1"
                     /translation="MSASDLTAANAAKPLAAPAAVQSLPASEVSGWRTPVELTLLGAV
                     WGGSFLFMRVAAADFGPFALVDLRLALGALILVPFLWRERARFTPGVWLRVFGIASIN
                     SVLPFVLFAWGAERAPAGIGAITNAMTVMFAALVAFLFYGEHIGPRRWIGLVAGFVGV
                     AILASGHIAGASVGAAALAGTAAALCYGLGINFVRHYLSGFPAAAIAAANLASGALLL
                     APLAIYTWPQHPVPPASWWSAVLLGVLCTGIAFVFYYRLIARIGAPRTATVTYLIPLF
                     GVIWAWLLLGEAVTLSMLLAGALILAGVGLSQQREAKREART"
BASE COUNT          598 a         1128 c         1200 g          461 t
ORIGIN      
        1 gacgtgcgcg tagcctcgag cgcaccgcgt agccatggca cactggtgat cgatacgaat
       61 gatcccgccg ccccgcacgg tcagacacgc gtggagttct cccccagatg agcagcctcc
      121 tcagatcggc tccgctgtca ctcctcgtca tcgtgctgag cttgccgatg acggccagac
      181 tcgccgcatc gcaggatgcg cgcgccgcgg gcgacacccc gactgcgaag cccagcgctc
      241 cgctcaagat tggcatcatc ggcacgggac atatcggcgg ggcgctcgcg acgctctggg
      301 tgggcgcggg gcacgaggtc ctgatggcct cccgtcatcc acaggagctg caggggcttg
      361 cgcactcgct cggacccagg gcgcacgtcg gcacgccgac ggaggcggcc cgattcgctg
      421 aggtcgtgct gatctcggtg ccctacgggg cgctgccgca ggtcgggcgc gagctgaaga
      481 acgagctcgc gggcaagatc gttctcgaca ccggcaaccc ctaccccgag cgcgatggcc
      541 ccatggcggt cgaggcgcga cgcaagggca ccggagtcgc ctcagcggag tatttgccgg
      601 gagtgcgcct ggtgcgggcc ttcaatgcga tcaactccgg cgacttgcgc agcgaggcgc
      661 atcgcaaggg ggcaccgatc gccatacccc tcgcgggaga cgacggcgag gcgctgcggg
      721 tcgcggagca attggtgaag gatgcgggtt ttgcaccggt cgtggtcgga ccgctggcga
      781 aagcgcgcga gttcgatgtc ggcacgccgg tgtacacgcg gctcatgacc gagccgcaac
      841 tgcggtcagc gctcgggctg aagtagcgcg cagccggctc aggtaaccgg cggcggcggc
      901 tgccgctcga ccttcgggat gcgctcatcg aagcacgccc agctcggtgc gctggaggtc
      961 cagatgctca tcgcgggttt cgccagctcc ggctcatcga gactgccggc gcgcacgaag
     1021 atcaggtgcg gccgggcggt ggtgccggtg aacaggtgtg ttccacacag cggacagaaa
     1081 cgccgctgca tggggttgcc actgtcggcc accgcgcggt actcgcgcag ctcgccgcgc
     1141 acatcgaggg cttcggccgg gaagcataca ttgaccgtgg cacccccggc gccgaggtac
     1201 tgacagacgc ggcaccagca ggtgcgcgca acgatgggcg gagcgctgat cgaataacgt
     1261 actgccctgc agaggcaacc gccggtgatg ttcatgggtg cgatgctcgg tggtgcgcgc
     1321 gcggcgtggg agcatcagca tagcggaagg aagcgcaccg gactgaatcg tcctcaaatc
     1381 cggagcgtgc tggaaaatgt gcccgcgcta tcaatctggg ccgctcggga gcacgttcat
     1441 ccggtgccag aataagtatg gtgcggcggc gctgaacgtg gcgtgaatgg ccacgacgac
     1501 gatggagcgg gttcagtccc gcaagcgcgc gcgcagacgc gcgatctcat cggccgagag
     1561 cccgatcttc gcgagataag cgcgcacacc tccggcgcgc tcgaggaaag cgagcatgtt
     1621 gtaggcacct tgctcggggc agcgcagcgc ctcgaggatc cgctcgcgct cggtgtgcgg
     1681 atagcgccgc agataaccct cgcgcagata ctcggcgctc accgagtaat cctgcgctat
     1741 ggccgccgga gtggcatcgg cgagcgcgag cagcaaagcg gcgatgagcc cggtgcggtc
     1801 cttacccgcg acacaatgaa acagcagcgg ctcgcgcggg gcggcggcga tgaagccgag
     1861 caccgtgcgc agctccgcgc gcacgtgctc gagcaccacg cacttccaca gttccttggc
     1921 cgcgtcgcgg ctgcgcagcc gccagtcatc ctcggtgtgg ctcagcagcg agatgcgggc
     1981 atagcgcagc tgcggcagac gatcgggcac cgggctcggg gcttgcgccg cttcttccgg
     2041 ccagcgcaga tcgagtatgg tgcgcacccc gtagtcggcg agcgcctgaa gcccgagatc
     2101 gctgagctgc gcgaggtcat ccgcgcgcag cagcgagcgc cagcgcgtct cagcgccgtc
     2161 ggtggtcgga tagccgccca ggtcgcgggc gttcagcagg tccggaaaat cgagcggccg
     2221 ccccggcacg gccgccactt gcgccttgtt cctcaggttc tagcctcgcg tttcgcctcg
     2281 cgctgctggc tcaggccgac cccggcgagg atcagggccc cggcgagcag catgctgagc
     2341 gtgacagcct caccgagcag cagccatgcc cagatgacgc cgaacaacgg tatcaggtaa
     2401 gtgaccgtcg cggtacgcgg cgcaccgatg cgggcgatga ggcggtagta gaagacgaag
     2461 gcgatgcccg tgcacaagac accgagcagc accgcactcc accaggaggc gggtggaacc
     2521 ggatgctgcg gccaggtgta gatcgcgagc ggtgccagca gcagcgcgcc acttgccagg
     2581 ttggcggcgg cgatcgcggc tgccgggaag ccggagagat agtggcgtac gaagttgatg
     2641 ccgagaccgt agcacagtgc cgccgcggta ccggcgagcg ccgcggcccc gacactcgcg
     2701 cccgcgatgt ggccgctggc gaggatcgcc accccgacga agccggcgac gagaccgatc
     2761 cagcggcgcg ggccgatgtg ctcgccgtag aaaaggaacg cgaccagcgc ggcgaacatg
     2821 accgtcatcg cgttggtgat ggcgccgatg ccggccgggg cacgctcggc gccccaggcg
     2881 aagagcacga acggcagcac cgagttgatg ctggcgatgc cgaacacgcg cagccacacc
     2941 ccgggtgtga agcgtgcccg ctcgcgccac aggaatggca cgaggatcag cgcgccgagc
     3001 gcgagccgca ggtcgaccag cgcgaacggt ccgaagtcgg cagccgcgac gcgcatgaaa
     3061 agaaacgagc cgccccagac ggcgccgagc agcgtgagct ctaccggcgt gcgccagccc
     3121 gacacctcac tcgcagggag gctctgcaca gcggccggcg cggcgagcgg cttggccgcg
     3181 ttcgcggcgg tgagatcgga agcggacatg aacgttgcac ccttctgtcg tgaacatctg
     3241 cggtgaccgc taccgtgcca ggggactcag ctccgccgcc acccgatgct tgccgacacc
     3301 gccggcgcgg tcacccgagc gcctggcgga aatcctcgag cagatcatcg ggcgcctcga
     3361 tcccgaccga gaggcgcagc aatccat
//
LOCUS       DATDMP010000009         3373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1001011, whole genome shotgun sequence.
ACCESSION   DATDMP010000009 DATDMP010000000
VERSION     DATDMP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001011"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1675
                     /locus_tag="VI195_00135"
     CDS             71..1675
                     /locus_tag="VI195_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY6822817.1"
                     /translation="MRTAMRCITALLALTLGIAGCARHAAQPSGTVLIAMADNTFSPS
                     VVRVPVGGSVLFHNAGRSAHNAVAIDNSWSTERTFGNLSIPPEQMTEVMFPTPGVYRF
                     YCTFHGTPDGKGGMVGVIVVGDVQYTPPAGDRGVLPVVDRPSGVTRRVPVEYPNIQTA
                     VDAADPGDLIVVDKGVYAESVFVTTPSITIRGVDRNEVILDGQFALGSGIVVAANGVA
                     IENMTARNFTLNGFYWTGVNGFRGSYLTAYNNGDYGIYGFGVSNGVFEHSYASGSPDS
                     AFYIGQCYPCKVVLDDVTGAYSGLGYSGTNSGGEMYIVRSRFAHNRSGLSTTTFDIEL
                     YPPGRETTIVGNLITDSGLDNEAAGFYATETLAGNGIALVGTHANLVERNYIARSRNN
                     GILVLPLLDRHYWPSTRHVVRDNTVVSSGRADLAAGGFGTLHNCFAANRYRTSLPWGL
                     EILNGCGRMRIPLASDLSGDMTFFGSIAQVFAGRFNVLDYRNRPAPPPQPNMPGGPQA
                     PVAPAVHPFDDHKLALDSIPTPRESP"
     gene            1675..2010
                     /locus_tag="VI195_00140"
     CDS             1675..2010
                     /locus_tag="VI195_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822818.1"
                     /translation="MPSLFSMAHPISAFQVWFVVYVYLLPILLYGVWAALSLMDAIES
                     KQASWLAVLGVILLPLLGGAWYLLKHARTLSRTARVATVVAGTLVWLIPLIVAVWLVV
                     RPLGPKALS"
     gene            2064..2384
                     /locus_tag="VI195_00145"
     CDS             2064..2384
                     /locus_tag="VI195_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEY6822819.1"
                     /translation="MASSTIAFPTVLQMLLGLYGYLLPTLLYVLWSTLALWDLGRRAE
                     ARPTFTWAWTLAIFLVPFVGAIAYLLFGGGQLSPRSRMVAVGGGAAAYLLILLLGAAA
                     GGVS"
     gene            2384..>3373
                     /locus_tag="VI195_00150"
     CDS             2384..>3373
                     /locus_tag="VI195_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY6822820.1"
                     /translation="MRVGRRDLFRLGSVAGACGFLGALAWPVTRKLTARPAQSAAAAA
                     LLPIDCASEAGAKDSALAYAARYSPSPYLPNGSRDALHVPPPPRNGASGELLEVDLPI
                     IETTLEVANGRNVRVWAYGGTVPGPILRATLGDRLRITVTNHASVAHSTHFHGAHGVM
                     QDGIGRIAPGEKHVYELAAGPIGLHPYHCHVPPYACHIGKGMYGALIVDPVGGRAPAH
                     EFVLCLCGFDVEGSGRNDTYAWNGVAGYYDRFPLKVPVGELVRVYLLNMVEHDSIASF
                     HLHAHTFDVFRSGTSLTPHEHTDIVSLGPAERAILEFRLPQRGRYMFHPHQSHM"
BASE COUNT          556 a         1111 c         1055 g          651 t
ORIGIN      
        1 ggcttaaggt aatttgcaga gataagtact cgaagcggct ccaagcgcct gcaactgcgg
       61 ggacacgaga atgcgtacgg cgatgcgctg catcacggcg ttgctcgccc tgaccttggg
      121 catcgctggc tgcgcgcggc acgcggccca gccctccgga accgtcctca ttgcgatggc
      181 ggacaatacc ttctcaccga gcgtcgtgcg cgttccggtg gggggctcgg tgcttttcca
      241 caacgccgga cgcagcgcgc acaacgcggt cgccatcgat aactcgtggt cgaccgagcg
      301 caccttcggc aacctcagca taccgccgga gcagatgacg gaggtgatgt tcccgactcc
      361 cggggtgtat cggttttact gcaccttcca cggaacgccc gacggcaaag gcggcatggt
      421 cggggtgatc gtggtgggcg atgttcagta cacgccgccc gccggcgatc gcggcgtgct
      481 gcccgttgtc gatcggccga gcggtgtcac acggcgcgtc cccgtggaat atccgaacat
      541 tcagacggcg gtggacgcgg ccgatcccgg tgatctcatt gtcgtggaca agggagtcta
      601 tgcggagagc gtgttcgtga cgacgccgtc catcacgatt cgcggcgtcg accgcaacga
      661 ggtgatcctc gacggccagt tcgcgctcgg cagtggcatc gtagtggcag cgaacggcgt
      721 ggccatcgag aacatgacgg cgcgcaactt tacactcaac ggcttctact ggaccggtgt
      781 caacggattt cgcgggtctt atctgaccgc atacaacaac ggcgactacg gcatttacgg
      841 cttcggggta agcaacggcg tgttcgagca ctcctacgcg agcggctcgc cggattcggc
      901 cttctacatc gggcagtgct acccgtgcaa agtggtgctg gatgacgtca ccggtgccta
      961 cagcggactc ggctattccg gcaccaattc cggcggcgaa atgtacatcg tcaggtcgcg
     1021 cttcgcgcac aaccgcagtg ggttgagcac gacgactttc gacatcgagc tctatccgcc
     1081 cggccgcgaa acgacgattg tcggtaatct cattaccgac agcgggctcg acaacgaggc
     1141 tgccggcttc tacgcaaccg aaacccttgc cggcaacggc atcgctctgg ttggcacgca
     1201 cgcgaacctg gtggagcgca actacatcgc tcggagccgc aacaacggta tcctcgtgct
     1261 gccgctgctc gaccggcact actggccctc gacccgtcac gtggtacgcg acaacacggt
     1321 cgtgagctcc gggcgcgccg atctcgcagc cggcggtttc ggcacgctcc acaattgctt
     1381 cgcggcgaat cgctaccgca cttcacttcc ttgggggctg gagattttga acggctgcgg
     1441 ccgcatgcgc ataccgctcg cctccgacct atccggcgac atgacctttt tcggctccat
     1501 cgcccaggtg tttgcgggaa gattcaacgt gctcgactat cgcaatcgtc ccgcgccacc
     1561 gccgcagccc aacatgccgg gcggaccgca ggctccagtc gcacccgcgg tacatccctt
     1621 cgacgaccac aaactcgcgc tcgactccat acccacaccg cgggagtctc cgtaatgccg
     1681 tcgctcttct ccatggccca tccgatcagc gccttccagg tctggttcgt cgtctacgtt
     1741 tacctgctgc cgatcctgct gtacggggtg tgggctgcgc tgtcgctcat ggacgcgatc
     1801 gaatccaaac aggcgagctg gctggccgtg ctcggcgtga ttctgctgcc gctcctgggc
     1861 ggtgcctggt acctgctgaa gcacgctcgt accctctcgc gcacggcgcg tgtggccacg
     1921 gtcgtcgccg gaacgctcgt ctggctcatt ccgctcattg ttgcggtctg gctggtggtc
     1981 cgtccgctcg gaccaaaagc gcttagctaa agcagcgccg tccgcaagca ccgttaaact
     2041 gacaaagcgc aaagaggccg gtcatggcga gttcaacgat cgcatttccc acggtcctgc
     2101 agatgctcct cgggctctac ggctacctac tgcccaccct gctctacgtt ttgtggagca
     2161 cgctggcgct ctgggacctg gggcgcagag cggaggcccg gcccacgttc acctgggctt
     2221 ggacactagc gatattcctg gtgccctttg tcggtgcgat cgcctacctg ttattcggtg
     2281 gcgggcaact ttcgccacgc tcgcggatgg tcgcggtcgg cggcggcgcc gctgcgtacc
     2341 tcctgattct gctgctcggg gccgcagcgg gcggagtgag ctgatgcgcg tcgggcgccg
     2401 cgatctgttt cgcttgggct ccgttgccgg tgcctgcggc ttcctgggtg cgcttgcctg
     2461 gccggtgacg agaaagctga ctgcacggcc ggcgcaatcc gcagcggctg cggcgctcct
     2521 gcccatcgac tgcgcgagcg aagctggcgc gaaagattcg gcgctcgcgt acgcggcgcg
     2581 ctactcaccc tcgccttacc tgccgaacgg ctctcgcgac gctctgcatg tgccgccgcc
     2641 gccgcgcaac ggcgcgtccg gagagctgct ggaggtcgat ctgcccatca tcgaaacaac
     2701 gctggaggtc gccaacgggc gcaatgtgcg cgtctgggcg tacggcggta ccgtgccagg
     2761 cccgattctg cgcgccacgc tcggggatcg cctgcggatc accgtaacca atcacgcctc
     2821 agtggcccac agcacgcact ttcacggcgc gcacggcgtg atgcaggatg gaatcggccg
     2881 gatcgctccg ggagagaagc acgtgtacga gctggcagct ggcccgattg gcctgcatcc
     2941 gtaccactgc cacgtgccgc cctatgcctg ccacattggc aagggaatgt atggcgcgct
     3001 gattgtcgac ccggtgggcg gccgcgcgcc ggcacacgag ttcgtgctat gcctgtgcgg
     3061 gttcgatgtg gaggggagcg ggcgtaacga cacgtacgcg tggaatggag tcgccgggta
     3121 ctacgatcga tttccgctca aggtaccggt cggcgagctc gtgcgtgtct atctgctcaa
     3181 catggtcgag cacgattcga tcgcctcgtt tcacctgcac gcgcatactt tcgacgtttt
     3241 caggtccggt acctctctga cgcctcacga gcataccgat atcgtgtcgc tgggaccggc
     3301 cgagcgcgcg atactcgagt tccgtctgcc gcaacgcggc cgctacatgt ttcatccgca
     3361 tcagagtcac atg
//
LOCUS       DATDMP010000010         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1445715, whole genome shotgun sequence.
ACCESSION   DATDMP010000010 DATDMP010000000
VERSION     DATDMP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445715"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /gene="dctP"
                     /locus_tag="VI195_00155"
     CDS             <1..397
                     /gene="dctP"
                     /locus_tag="VI195_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY6822821.1"
                     /translation="YTGLQQGVIDGAENDSVTYFTTKHYEVAKNYALTSHLMLINTLV
                     LSEKQWQRLPENLRTVMRHASLEGRKLTLQVRAEKEAKVLDDLKSAGVTITRPDLKPF
                     IAAGRSTWAESEQRLGKELIQKISAAAGH"
     gene            404..922
                     /locus_tag="VI195_00160"
     CDS             404..922
                     /locus_tag="VI195_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY6822822.1"
                     /translation="MAALRSFILGLDRLLGRLEAAALAAMVLAITAVTFAQVFTRYVT
                     ETPLIWTEEVARYLFVWITLVGAAAAVRSNGHFGLDLLRRHFPLLRKPLGAATMLAVG
                     AFFALLLWTGIAETRQAALQMSPALQVGMQWAYLALPVGAALGLFHVIAHWARSGIAA
                     HPLDGHVDEGSM"
     gene            938..2224
                     /locus_tag="VI195_00165"
     CDS             938..2224
                     /locus_tag="VI195_00165"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY6822823.1"
                     /translation="MTASLAAFIAFIALGVPIAFALGTAAAIGLWMIEGAPLTMIASR
                     TFGAVDSFTLLAIPFFILAGELMETGGISQRLIVLARALVGHMRGGLGNVTIFGMVLF
                     SGISGSTQADAAAVGSLMIPAMKHHGYSAGRAGAIVAAAAGMGILVPPCLTMVVYGSV
                     TNVSIGALFAAGFLPAFAMAIALAVQQMLSARRAGLEPEPRAAWGERLRAIGSAFWAL
                     MMPVIIFGGILGGIFTPTEAAVVAAVYALFIGAFVYREISWSFLYRTLVRTGVITGVV
                     MIMIAGANIFSWLMTVERVPHALATLIQAVGGGRQVFILLSVLVFLVLFALLDGLPGM
                     LMIVPVFVPIAQQLGIDLLHYGIIMTTVMGIALFLPPFGVGLFIVLGIAGCPLRDITR
                     ELMPYVLTMLVVALVIAYVPAISFALPSAFGLYKAP"
     gene            complement(2209..>2545)
                     /locus_tag="VI195_00170"
     CDS             complement(2209..>2545)
                     /locus_tag="VI195_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEY6822824.1"
                     /translation="DATNHSEMRVLREASSTRAPPVLARATLYASTEPCAMCAGAAYW
                     AGIGRVVFGFSEASLRAMTGAHPWNPTLALPCRELFARGSRRIEVIGPYLEVEAAEPH
                     RGFWTQGAL"
BASE COUNT          351 a          884 c          858 g          452 t
ORIGIN      
        1 ctacaccggg ctgcagcagg gcgtgatcga cggcgccgag aacgactcgg tcacctactt
       61 caccaccaag cactacgagg tggcgaagaa ctatgccctg accagccacc tcatgctgat
      121 caacacgctc gtcctcagcg agaagcagtg gcagcggctc cctgagaatc tgcgcaccgt
      181 catgcgccac gcttcgctcg aggggcgcaa gctcacgctc caggtgcgcg ccgagaagga
      241 ggcgaaggtg ctcgacgacc tgaagagcgc gggcgtcacg atcacgcgtc cggacctgaa
      301 gccgttcatc gccgccgggc ggagcacctg ggcggaatcg gagcagcgcc tgggcaagga
      361 gctcatccag aagatctcgg ccgccgccgg tcactgacag ggggtggcgg cgcttcgcag
      421 cttcatcctc ggcctcgacc ggctgctcgg ccggctcgag gcggcggcgc tcgccgccat
      481 ggtgctcgcc atcaccgcgg tcaccttcgc gcaggtgttc acgcgctatg tgaccgagac
      541 cccgctcatc tggaccgagg aggtggcgcg ctaccttttc gtctggatca cgctggtggg
      601 ggcggctgcc gcggtgcgga gcaacggaca cttcgggctg gatctcctgc ggcgccactt
      661 ccctcttctt cgcaagccgc tcggcgcggc gacgatgctc gcggtgggcg cgttcttcgc
      721 gctgctgctg tggaccggca tcgcagagac gcggcaggcg gcgctgcaga tgtcgcccgc
      781 gctgcaggtg gggatgcaat gggcctacct cgccctgccg gtcggcgccg cgctggggct
      841 ctttcacgtg atcgcgcact gggcgcgcag cggaatcgcc gcgcatccgc tcgacggcca
      901 tgtggacgaa ggctcgatgt gacggacgag gcttgaaatg accgcctcgc tggcagcgtt
      961 catcgccttc atcgcgctcg gcgtgccgat cgcgtttgcg ctcggcaccg cggcggcgat
     1021 cggcctttgg atgatcgagg gcgcgccgct caccatgatc gcgagccgca ctttcggcgc
     1081 ggtggacagc ttcacgctgc tcgccatccc gttcttcatc cttgcgggcg agctgatgga
     1141 gaccggcggc atctcgcagc ggctcatcgt gctcgcgcgc gcgctggtcg gccacatgcg
     1201 cggcgggctc ggcaacgtca cgatcttcgg catggtgctg ttttcgggca tctcgggctc
     1261 gacgcaggcc gacgcggcag cggtcggctc gctcatgatc ccggcgatga agcatcacgg
     1321 ctacagcgcc ggccgggccg gcgcgatcgt cgccgccgcc gccgggatgg gcatcctcgt
     1381 gccgccctgc ctcaccatgg tggtctacgg cagcgtcacc aacgtctcga tcggcgcgct
     1441 cttcgccgcc ggattcctgc cggccttcgc catggcgatc gcgctcgcgg tgcagcagat
     1501 gctgagcgcg cgccgcgccg ggctcgagcc cgagcctcgg gccgcctggg gcgagcgcct
     1561 gcgcgccata ggctccgctt tctgggcgct catgatgccg gtgatcatct tcggcggcat
     1621 cctgggcggc atcttcacgc cgaccgaggc ggcggtggtc gcggcggtct acgcgctttt
     1681 catcggcgcc ttcgtctacc gcgagatcag ctggagcttt ctctatcgta cgctggtacg
     1741 cacgggcgtc attaccggcg tggtgatgat catgatcgcc ggcgcgaaca tcttctcgtg
     1801 gctgatgacc gtcgagcgcg tgccgcacgc gcttgccacg ctcatccagg ccgtgggcgg
     1861 cggacgccag gtgttcatcc tcctcagcgt gctggtgttc ctcgtcctct ttgcgctcct
     1921 cgacggcctt ccgggcatgc tgatgatcgt gccggtgttc gtgccgatcg cgcagcagct
     1981 cgggatcgat ctcctgcact acgggatcat catgaccacg gtgatgggaa tcgccctttt
     2041 cctgccgccc ttcggggtgg gcctcttcat cgtcctcggc atcgcaggct gcccgctgcg
     2101 cgacatcacg cgcgagctca tgccctatgt cctgaccatg ctcgtggtgg cgctggtgat
     2161 cgcctacgtg cccgccatca gcttcgcgct gccctctgcg ttcggcctct acaaggcgcc
     2221 ctgagtccag aaaccgcgat ggggctcggc cgcctcgacc tccagataag gaccgatgac
     2281 ctcgattcgg cggctgccgc gcgcgaaaag ctcgcggcac ggcagcgcga gggtgggatt
     2341 ccatggatgc gcgcccgtca tcgcgcgcag tgacgcctcg ctgaagccga acaccaccct
     2401 gccgatgccc gcccaatagg cggcgccggc gcacatggcg cacggctcgg tgctcgcgta
     2461 gagggtcgcg cgcgcaagca ccggcggcgc acgcgtggac gatgcctcgc gaagcacgcg
     2521 catctcggaa tggttggtgg cgtcg
//
LOCUS       DATDMP010000011         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1223567, whole genome shotgun sequence.
ACCESSION   DATDMP010000011 DATDMP010000000
VERSION     DATDMP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223567"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VI195_00175"
     CDS             complement(<1..1359)
                     /locus_tag="VI195_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6822825.1"
                     /translation="MPSLAEIPTEKSAVTRAPDELTLGEALELAVRYHRADQLDVAES
                     VYRRVLEMVPEDPEALHFLGVLLHQRGKHDEALELLERAIVLQPERAGFHQNMGNVLF
                     EMERLTEAARSYERCLELAPDDPAGYNNLGVVRRAQRQPDAAAEAYRKAIEIDPRHPD
                     AYHNMGNLLTAQGRIKEAVTYLCTSMTLRPAHPESRRLLALAYANLGRLDEAVDVYRK
                     WLEEEPGDSIARHMLAACGGDAVPERATDQFVQMVFDSFALSFDAKLEKLHYRAPQLI
                     AAELARIRGTPEKSLVVLDAGCGTGLSAPLLAPFAARLIGVDLSAQMLAKARQRGGYD
                     ALLQSELTQYMRAHRAEFDLIVSSDTLCYFGALDAVLESARCALRGQGAVLFTVEQVE
                     DALVPDAGYRLNPHGRYSHSEGYVRRALAAAHFEVLGITQAMLRMESGCPVAGLIVSS
                     KTA"
     gene            complement(1380..>2308)
                     /locus_tag="VI195_00180"
     CDS             complement(1380..>2308)
                     /locus_tag="VI195_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6822826.1"
                     /translation="QTVLKLADTLDQRVIGQKHALEMIARRIQTSRARLDNPNKPIGV
                     FMLCGTSGVGKTETALALAELLYGGEQNAITINMSEFQEAHTVSTLKGAPPGYVGYGE
                     GGILTEAVRRRPYSVVLLDEVEKAHPDVHEIFFQVFDKGWMEDGEGRYIDFKNTIILL
                     TSNVGSELITSMCKDPELLPDPESLAKGLREPLLKVFPPALLGRLVVIPFYPLSDEVL
                     ANIVRLQLERIRKRVVANHKVPFEYGDDAVKLVVSRCTEGESGGRMIDAILTNTILPR
                     ISQEFLTRMVEGKPITRVKIAVEDQDFSYAFD"
BASE COUNT          384 a          763 c          745 g          416 t
ORIGIN      
        1 cgccgtcttg ctgctcacga tgagtcccgc cacgggacaa ccactttcca tgcgcagcat
       61 tgcctgcgtg atgccgagca cctcaaagtg cgccgcagcg agagctctgc gcacgtagcc
      121 ctcgctgtgg ctgtagcggc cgtgcggatt gaggcggtag cccgcgtccg ggacgagtgc
      181 gtcctcgacc tgctcgacag tgaagagcac cgcgccctgc cctcgcagtg cgcaccgtgc
      241 ggactcgagc accgcatcga gcgcgccgaa atagcacagc gtgtcggatg agacgatcag
      301 gtcgaactcg gcgcggtgcg cgcgcatgta ttgggtgagc tcgctctgca agagcgcgtc
      361 atagccgccc cgctggcgcg ccttggcgag catctgcgcc gacaggtcca cgccgatgag
      421 tcgcgcagcg aacggggcaa ggagcggcgc gctaagaccc gttccgcagc ctgcatcgag
      481 caccaccagc gatttctcgg gggttccgcg aatgcgcgcc agctccgcgg cgatgagctg
      541 cggggcccga tagtggagct tctcgagctt ggcatcgaag ctcagcgcaa agctgtcgaa
      601 caccatctgc acgaactgat cggtggcgcg ctccggtacg gcatcgccgc cgcaggcggc
      661 cagcatgtgc cgagcgatcg aatcgcccgg ctcttcctcg agccacttgc gatacacgtc
      721 cactgcctcg tccagtcgcc cgagattcgc gtaggcgagc gcgagcaggc gccgcgactc
      781 gggatgcgcc ggtcgcagtg tcatggaggt gcaaagatag gtgacggcct ctttgatgcg
      841 cccctgagcg gtgagcagat tgcccatgtt gtgataggca tcaggatggc gggggtcgat
      901 ctcgatcgcc ttgcggtagg cttccgccgc cgcatcgggc tggcgctgtg cccgccgcac
      961 cacgcccagg ttgttatagc cggccgggtc atccggggcg agctcgaggc agcgctcata
     1021 ggagcgcgcg gcctcagtga gcctctccat ctcgaagagc acgttgccca tgttctggtg
     1081 gaagcccgcg cgctcaggct gcaggacgat cgcgcgctcc agcagttcca gcgcctcgtc
     1141 gtgcttgccc cgctgatgca gcagcactcc gagaaaatgc agcgcctcgg ggtcctcggg
     1201 caccatctcc agcacgcgcc gatagacgga ctccgcgaca tcgagctggt ccgcgcggtg
     1261 atagcgcacg gcgagctcga gcgcctcccc cagggtgagc tcgtcaggcg cgcgggttac
     1321 ggcgctcttt tccgtgggaa tctcggcgag gctcggcatg ctcgatctcg gattgccgtt
     1381 caatcgaacg cgtagctgaa gtcctggtcc tcaacggcga tcttcactcg cgtgatcggc
     1441 ttaccctcca ccatccgcgt cagaaactcc tggctgatgc gcggcaggat ggtgttggtg
     1501 agaatcgcgt cgatcatgcg gccgccactc tccccttccg tgcagcggct cacgactagc
     1561 ttcacggcat cgtcgccata ctcgaatggc actttgtgat tggccaccac gcgcttgcga
     1621 atacgctcga gctgcagccg cacgatattc gccaggacct cgtcgctcag cgggtaaaag
     1681 gggatgacga ccaggcgccc cagcagcgcc ggcgggaata ccttgagcag tggctcccgc
     1741 agacctttgg cgagcgactc cggatcgggg agcagctccg gatccttgca catcgaggtg
     1801 atgagctcgg aaccgacgtt ggaagtcaac aggatgatgg tgttcttgaa gtcgatgtag
     1861 cggccttcgc cgtcctccat ccaccccttg tcgaacacct ggaaaaagat ttcgtgcaca
     1921 tccgggtgtg ccttctccac ttcatcgagc agcacgacgc tgtagggacg gcgccgcacc
     1981 gcttcggtga gaatgccgcc ctcgccgtag ccgacgtatc cggggggcgc ccccttcagg
     2041 gtcgagacgg tgtgcgcctc ctggaactcg ctcatgttga tcgtgatcgc attctgctcg
     2101 ccgccataga gcagctccgc gagcgccagc gccgtctcgg tcttgccgac cccggaggtg
     2161 ccgcacagca tgaacacgcc gatcggcttg ttcgggttgt ccaggcgggc gcgcgaggtc
     2221 tggatgcgcc gcgcgatcat ctccagcgca tgcttctgcc cgatgacgcg ctggtccagc
     2281 gtgtcggcca gcttgagcac ggtctgga
//
LOCUS       DATDMP010000012         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1001440, whole genome shotgun sequence.
ACCESSION   DATDMP010000012 DATDMP010000000
VERSION     DATDMP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001440"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VI195_00185"
     CDS             complement(<1..834)
                     /locus_tag="VI195_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY6822827.1"
                     /translation="MKKGASATRRGDGAPRDLLARLKDGAVICAEGYVFELERRGYLQ
                     AGAFVPEVLFEHPEVVEQLHLDFVRAGSDVTQALTYYVHREKLRVIGREKDLAPMNRT
                     ALGIAKRVARRTGTLFAGDLCNTNIYDPADSKSVKAVERIFDEQVGWAVEAGVDYFVA
                     ETLGWVGEALIALQAIRRNSKVPVVITFAMHREELTREGLSPAQACQRLEAAGADVVG
                     LNCHRGPRTMMPLLKEIRAAVKCHVAGLPVPYRTTPAQPSFMALTDPCCGDRMAFPDG
                     LD"
     gene            1073..>2039
                     /locus_tag="VI195_00190"
     CDS             1073..>2039
                     /locus_tag="VI195_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6822828.1"
                     /translation="MSAPLAEQDRRTWERLAAGVKPEGRAIIGGRAVEALDGRVFEDL
                     SPIDGRSICSVARGGPADIDTAVRAARQSFESGPWRRLGPRERKRILRRFAEAIRADA
                     QHLALLETRDVGKPIANSVSVDVANCADCIEYYAEFADKLYDEVAPTGPKDLALIRRE
                     PLGVVGAIVPWNYPLIISCWKVGPALLTGNSVVLKPAEQSPLSAIRLAQLALEAGIPP
                     GTFNVVPGYGEEAGKALALHQDVDLITFTGSTEVGRLMLHYAAESNLKRVALECGGKT
                     PQVVFADADLEAAASGIAWGIYYNSGQTCHAGSRLIVDQRVREPLL"
BASE COUNT          321 a          691 c          673 g          354 t
ORIGIN      
        1 gtcgagcccg tcaggaaagg ccatacggtc gccgcagcac ggatcggtga gcgccatgaa
       61 cgagggttgt gccggtgtgg tgcggtaggg taccggcagt cccgcgacgt ggcactttac
      121 cgctgcgcga atctccttca gcagcggcat catggtgcgt ggaccgcggt ggcagttgag
      181 gcccacgacg tcggcgccgg ccgcttccag gcgctggcag gcctgcgcgg ggcttagccc
      241 ctcgcgcgtc agctcctcgc ggtgcatcgc gaaggtgatc accaccggca ctttcgagtt
      301 gcgccgaatc gcctgcagcg cgatcagcgc ctcccccacc cagccgagcg tctccgctac
      361 gaagtaatcg acaccggctt cgaccgccca gcccacctgc tcatcgaaaa tacgctcgac
      421 agccttcacg gacttgctgt cggcgggatc gtagatgttg gtgttgcaca ggtccccggc
      481 gaagagcgtc ccggtacgac gggccacgcg cttggcgatg ccgagcgcgg tccggttcat
      541 gggcgccagg tccttctcgc gcccgatgac gcgcagtttc tcgcggtgca cgtagtaagt
      601 aagcgcctgc gtgacatcgg agcccgcgcg gacgaaatcc aggtgcagct gctcgaccac
      661 ctccgggtgc tcgaacagca cctcgggtac gaaggcgccg gcctggagat atccgcgccg
      721 ctcgagctcg aatacgtagc cctcggcgca gatgaccgcg ccatctttca gccgcgcaag
      781 caggtcccgc ggcgcaccgt cgccgcgtct cgtggcgctc gcgcccttct tcacgcccgg
      841 actgatgcgt ggcgcacgcc gggtggcttt tgcccggggc ttggtcctgg atcgggtttt
      901 ggctcgggcc ttcgctgcca tgggggtctc cgtgggcatg aaaatgctgc acgctcgttg
      961 aggggcgcgc tacattgtaa tcagcaccga tgtggtcccg atgccacttt tcgatggggc
     1021 tattcatttt ctcgactgca acatccgtct caaccatgga agcgtgataa ccatgagcgc
     1081 ccctctcgcc gagcaggatc gccgcacctg ggagcgcctc gcggcgggcg tcaaacccga
     1141 aggccgcgcc atcatcggcg ggcgcgcggt ggaggcgctc gatggacggg tgttcgagga
     1201 cctgagtccg atcgatggcc gcagcatctg ctcggtggca cgcggcggac cggccgacat
     1261 cgacacggcg gtgcgcgcgg cacgccagag cttcgagagc ggcccgtggc ggcggctcgg
     1321 gccgcgtgag cgcaagcgca tcctgcggcg cttcgcggag gcgatccgcg cggatgcgca
     1381 gcacctggcg ctgctcgaga cgcgcgatgt gggcaagccc atcgccaact ccgtgagcgt
     1441 cgatgtcgcc aactgtgccg actgcatcga gtattacgcc gagttcgccg acaagctcta
     1501 tgacgaggtc gcccccacgg gcccgaagga cctggcgctc atccgccgcg agccgctcgg
     1561 ggtggtgggt gcgatcgttc cctggaacta ccctctgatc atcagttgct ggaaggtcgg
     1621 gccggcgctc ctcaccggca actcagtggt actgaaacct gccgagcagt ccccgctctc
     1681 agccatccgc ctcgcgcagc ttgcgctcga agcgggcatc ccccctggaa ccttcaacgt
     1741 cgtgccgggc tacggcgagg aagccggcaa ggcgctcgcg ctgcatcagg atgtcgatct
     1801 cattaccttc accggctcca ccgaggtcgg tcggctcatg ctgcactatg cggccgagtc
     1861 caacttgaag cgcgtcgcac tcgaatgcgg cggcaagacc ccgcaggtgg tgttcgcgga
     1921 tgccgatctc gaggccgctg cttccggcat cgcctggggg atctattaca acagcggaca
     1981 gacctgccat gccggctccc ggctcatcgt cgatcagaga gtccgcgagc cgttgctcg
//
LOCUS       DATDMP010000013         5080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1870, whole genome shotgun sequence.
ACCESSION   DATDMP010000013 DATDMP010000000
VERSION     DATDMP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..992
                     /locus_tag="VI195_00195"
     CDS             366..992
                     /locus_tag="VI195_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822829.1"
                     /translation="MSDAAKRVLPVANLREFFRDTLHGALVRQHLAVEDQTEHYVVNL
                     LTLFARSEALFESTPDGLRLKPLVAMLCEALEAAPGTAERYRYLQRLGDVSLFVAGFF
                     AQGFATRLIDIDYHIAMGGRAYGALAQCSARGRGRVLAAVFAELSEKFQPLVDALNEV
                     SESSGAQSDRDILRLYEIWLKTGSARCYAQLKRLGVDPTSAAGTAFAH"
     gene            997..1674
                     /locus_tag="VI195_00200"
     CDS             997..1674
                     /locus_tag="VI195_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822830.1"
                     /translation="MLLSRLQSLIGGIYDVSVAHDVYDFLVTDRQRLPAAARGGTAEE
                     ELIVAQPAEGDDEVALSLYLDPALLGRLTREDPLVRLHAGNVADWCTALEGVSHFLYL
                     AWNAGHDKPVSLLELEMQAEVDKYVASYWLLRRQFPQHFPAELRHVLFERTRVDPRAA
                     AAGRAELYREASRYADKFCARLERLLSRSRAARSGAAADEAVLTELRRFYRLTHARKR
                     ALIEGIG"
     gene            complement(1783..2142)
                     /locus_tag="VI195_00205"
     CDS             complement(1783..2142)
                     /locus_tag="VI195_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822831.1"
                     /translation="MSNNPRKISPEQAASAAERRRIGTVVHDDRGNASVEWRAAPVDG
                     ERQVLEVLGEDGLTLKTEELSYDPYARQRPMRAGGSRSAPAGGNRRTDLRKLSEWIKK
                     MRELEERKRLDAEPDET"
     gene            complement(2226..2777)
                     /locus_tag="VI195_00210"
     CDS             complement(2226..2777)
                     /locus_tag="VI195_00210"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6822832.1"
                     /translation="MKPDKQTLQFAGRVVRVTTDEVVLPNGHRTLLEVVHHPGGAAAV
                     ALDDAGRVCLLRQYRYVAGGWLWEVPAGKLEPGEPPLATAQRELSEEAGVRAQQWDSL
                     GSCLSSPGVFTERLHLFLATGMVPACAAQEHAEVIEIHWLPFDEACRWAIDGTIADCK
                     TALGLLRAREHLRTNGRARDTIP"
     gene            2845..4941
                     /locus_tag="VI195_00215"
     CDS             2845..4941
                     /locus_tag="VI195_00215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY6822833.1"
                     /translation="MATTTSHLDRLNGPQRKAVTHGEALPEKGYRAGPLLIIAGAGTG
                     KTDTLAHRVAHLVINGVDPARILMLTFTRRAALEMRRRAHEITRKALDDTLGGITQSI
                     AQRLSWAGTFHSVGNRLLRHYASHLQLDPHFSVIDRTDAADLMDSLREELGLAQKEQR
                     FPRKDTCLAIYSNRVNTRAPLAEVLQQQYPWCVQWEAELTGLYRAYVEQKQHANLLDY
                     DDLLLYWHGMMAEPRLAQHIGTHFDHVLVDEFQDTNRLQAEILYAMKPDGAGLTVVGD
                     DAQAIYSFRAAAVENILGFPERFNPPAEVVTLAQNYRSTQQVLDVANAVMAEAPRQHR
                     KYLLSIRGVGARARAVTVDDLQSQAEFICTEVLKKREASVPLKRQVVLFRSASHSDLL
                     EVELAKRKVPFVKYGGLRFLEAAHIKDLLGVLRWADNPRNALAAFRTLQLLPGIGPVH
                     ARAAIEYLERSGHSFGGLSGFTLPQQTPEIDWRRLSELMQSLADPQRQWAGQLHMVRE
                     WYQRYFERLYEDHHTRQGDLDQLELLSGQFPSRERFVTELTLDPPHATSDLAGRPTLD
                     DDYLVLSTIHSAKGMEWDSVYVLNVVDGSFPSEFSTGKAELIEEERRLLYVALTRAQN
                     DLAVMVPLKFHLTHQSRQGDAHVYGGRSRFMTERVLKTLEALTFHGSASAGSDALQDS
                     SSTVTLDVGARLREMW"
BASE COUNT          825 a         1747 c         1639 g          869 t
ORIGIN      
        1 tgcgcaccgt gtggatgaac cgccgcgcgc tcgcgtggcc cccgcagctg ccgcccgcag
       61 atctcacggt gagcgattgc gcgcagctcg cggccgcgct gcgcagctga cgcgcagcct
      121 gcgatcgggg cagagcctgc gcgggcgctc gccccggggc cgcgtcgcgt gacgcagtca
      181 gcaattctga ctcccctact gccgtccaac atccctgtca gcactcgtgc agcgaggctg
      241 ccaacgtcag ttttcaccca cgcgttcatt cggcgcagtc ggtcacggaa atccggcagc
      301 gcagcagtga accttccacc acggctgtcg ttccatataa gttaaggagg aggaggcgag
      361 cgacgatgag cgatgctgca aagcgtgttc tgccggtagc gaatctgcgc gagttcttcc
      421 gcgacaccct gcatggcgcg ctggtgagac aacatctggc ggtcgaggat cagaccgagc
      481 actacgtcgt caatctgctc acgctgttcg cgcgctccga ggcgctcttt gagagcactc
      541 ccgatggact gcggctgaag ccgctggtcg cgatgctgtg cgaagcgctc gaagcggcac
      601 ccggtaccgc cgaacgctac cgctatctgc agcggctcgg cgatgtgtcg ctgttcgtcg
      661 ccggcttctt cgcacagggc ttcgccaccc gcctgatcga catcgactac cacatcgcca
      721 tgggcgggcg cgcctacggt gcgctggcgc agtgcagcgc acgcggccgc ggacgggtgc
      781 tggccgccgt gtttgccgag ctgtccgaga agttccagcc gctggtggat gcgctcaacg
      841 aggtgagcga gagctccggt gcgcagtccg accgcgacat cctgcgtctg tacgagatct
      901 ggctgaagac cgggagcgct cgctgctacg cgcagctgaa gcgcctcggt gtcgacccga
      961 ctagcgccgc cgggacggca ttcgcccact gagcgcatgc tgctttcgcg gctgcagagt
     1021 ctcatcggcg gcatctacga tgtgagcgtc gctcacgacg tctacgactt cctcgtcacc
     1081 gaccggcagc ggctgcccgc agccgcgcgt ggcggtacgg ccgaggagga gctgatcgtc
     1141 gcccagcctg ccgagggtga cgatgaggtc gcgttgtcgc tgtacctcga tccggcgctg
     1201 ctcggacggc tgacgcgcga ggatccgctc gtgcgcctgc atgccggcaa cgtcgccgac
     1261 tggtgcacgg cgctcgaggg cgtgagccat ttcctctatc tcgcctggaa cgccgggcac
     1321 gacaagccgg tgtcgttgct cgagctggaa atgcaggcgg aggtcgacaa gtacgtggcg
     1381 agctactggc tgctgcgccg ccagtttccg cagcactttc cggcggagct gcggcacgta
     1441 ctgttcgagc gcacgcgcgt cgatccgcgt gccgcggcag cgggacgcgc tgagctgtac
     1501 cgcgaggcca gccgctatgc ggacaagttc tgtgcgcgtc tcgagcggct gctgtcgcgc
     1561 agccgcgcgg cacgcagcgg cgccgcggcc gatgaggcag tgctcaccga gttacgccgg
     1621 ttttatcgcc tgacgcacgc gcgcaagcgc gccctcatcg agggcatcgg ctgaaagcac
     1681 cccaagaaat cacgcttcac gcgatctgcg agctatgcgc cgatcagcaa gaggcgtgat
     1741 ttctcggggg ccccggcgag gcgccgccgc cgcgacctgc catcaggtct cgtccggctc
     1801 tgcgtccagg cgcttgcgct cctcgagctc gcgcatcttc ttgatccact cggagagctt
     1861 gcgcagatcg gtgcgccggt tgccaccggc cggcgcgctg cggctacccc cggcacgcat
     1921 tgggcgctgg cgtgcgtacg gatcgtagga gagctcctcg gtcttcaagg tcagcccgtc
     1981 ctcgccgagc acctcgagta cctgacgctc cccgtctacg ggcgcagcgc gccactcgac
     2041 cgaggcgttg cctcggtcat cgtgcacgac cgtgccgatg cggcgccgct cagcggctga
     2101 cgctgcctgc tctggactga tttttcttgg attgttgctc acggattatg tactaaccca
     2161 cttcagggcc tgcttcccct ggtcgcagcg tacacacgtg cacagccgcg attccgcgag
     2221 ctaaatcacg gaatagtgtc gcgcgcccta ccgttcgtcc gaaggtgctc gcgcgcgcgc
     2281 agcagcccga gcgccgtctt gcagtcggcg atggtgccgt cgatggccca gcggcaggcc
     2341 tcatcgaaag gcagccagtg gatctcgatg acctcggcat gctcctgtgc cgcacatgcc
     2401 ggcaccatgc cggtcgcgag gaacaggtgg agtcgctcgg tgaacacgcc gggggagctg
     2461 agacagctgc cgaggctgtc ccattgctgt gcgcgcactc cggcctcttc gctcagctca
     2521 cgctgcgcgg tggcgagcgg tggctcaccg ggctcgagct tgccggccgg cacctcccac
     2581 agccagccgc cggccacgta acggtactgc cgcaacagac acacccgccc ggcatcatcc
     2641 agcgccaccg ccgctgcgcc accggggtga tgcaccactt ccaacagcgt gcggtggccg
     2701 ttcggcagca ccacctcgtc ggtggtcacc cggaccaccc gcccggcgaa ttgcagggtc
     2761 tgtttgtcag gtttcatgac gtacatcgta gctgctggcg gcagcgctcg tgtacgaaag
     2821 ggtatagttg gctgctatac gcccatggcc acgacgacgt cgcatctcga ccggctcaac
     2881 ggcccgcaac gcaaggctgt gactcacggc gaggcgctac ccgagaaagg gtaccgcgcc
     2941 ggtccgctgc tgatcattgc cggcgccggt accggcaaga ccgacacgct cgcccatcgc
     3001 gtcgcccacc tggtcatcaa cggcgtcgat ccggcgcgta tcctcatgct caccttcacg
     3061 cgccgcgccg ccctcgagat gcgccgccgc gcccacgaga tcacccgcaa ggctctcgat
     3121 gacacactcg gcggcataac gcagagcatt gcccagcgcc tcagctgggc gggaaccttc
     3181 cattccgtcg gtaaccgcct gctgcggcac tacgcgagcc acctgcagct cgatccgcac
     3241 ttcagtgtca tcgatcgcac cgacgcggcg gatctcatgg acagcctgcg cgaggagctc
     3301 ggcctcgccc agaaggagca gcgcttcccg cgcaaggaca cttgcctggc gatctactcc
     3361 aaccgcgtca acacgcgcgc gccgctcgcg gaagttctgc agcagcagta tccctggtgc
     3421 gtgcaatggg aggccgagct caccgggcta taccgcgcgt acgtcgagca gaagcagcac
     3481 gcgaatctgc tcgactacga cgacctgctg ctctactggc acggcatgat ggccgagcca
     3541 cgccttgcgc aacacatcgg cacgcacttc gatcacgtgc tggtcgatga attccaggac
     3601 accaaccgcc tgcaggccga gattctctac gccatgaagc ccgatggggc cgggctcacg
     3661 gtggtgggcg acgatgccca ggcgatctac tccttccgtg ccgcggcggt cgagaacatc
     3721 ctcggctttc ccgagcgctt caacccgccc gcggaggtgg tcaccctggc gcagaactac
     3781 cgctcgactc agcaggtgct cgatgtcgcc aacgcggtga tggccgaggc gccccgccag
     3841 caccgcaagt acctgctgtc gatccgcggt gtgggggcgc gtgcgcgcgc ggtcacggtg
     3901 gatgatctgc agagccaggc ggagttcatc tgcaccgagg tgctgaagaa gcgcgaggcg
     3961 agcgtgccgc tcaagcgcca ggtggtgctg ttccgcagtg ccagccatag cgacctcctc
     4021 gaggtggagc tcgcgaagcg caaggtgcca ttcgtgaaat acggtgggct gagattcctc
     4081 gaggctgcgc acatcaagga tctgctcggg gtactgcgct gggcggacaa tccgcgcaac
     4141 gcgctcgctg ccttccgcac gctgcagttg ttgcccggca tcggtccagt gcacgcgcgt
     4201 gcggcgatcg agtatctcga gcgcagcggg cactcattcg gcggcctgag cggcttcacc
     4261 ctgccgcagc agacaccgga gatcgactgg cggcggctga gcgagctcat gcagtcactc
     4321 gccgatccgc agcgccagtg ggcgggtcag ctgcacatgg tgcgcgagtg gtaccagcgc
     4381 tatttcgagc gcctgtacga ggaccatcac acgcgccagg gcgatctcga ccagctcgag
     4441 ctgctctccg ggcaatttcc ctcgcgcgag cgcttcgtca ccgagctcac cctcgatccg
     4501 ccgcacgcca ccagcgacct cgccgggcgg ccgacgctcg atgatgacta tctggtgctc
     4561 tcgaccatcc actccgccaa gggcatggag tgggacagcg tgtatgtgct caacgtcgtc
     4621 gatggcagtt tcccttccga attctccacc ggcaaggcgg agctgatcga ggaagagcga
     4681 cgcctgttgt acgtggcgct gacccgcgcg cagaacgacc tcgccgtcat ggtgccgctg
     4741 aagttccacc tcacccatca gtcgcgtcag ggcgatgcac acgtttatgg cggccgcagc
     4801 cgtttcatga ccgagcgcgt gctgaagacg ctcgaggcgc tcaccttcca cggctcggcg
     4861 tcggccggca gcgatgcgct gcaggacagc agctccaccg tcacactcga tgtcggtgca
     4921 cggctgcggg agatgtggta ggcgacgcgg taccctcagt gggtcggttt cacgaagcgc
     4981 agcgtgaaac ggtcgctctc gccgatggcg gcatacttgt cgcgatcctt ggctccgagg
     5041 cgataggtcg gtggcagtgt ccacactccc tgctcgtagt
//
LOCUS       DATDMP010000014         6772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1112768, whole genome shotgun sequence.
ACCESSION   DATDMP010000014 DATDMP010000000
VERSION     DATDMP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112768"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VI195_00220"
     CDS             complement(<1..288)
                     /locus_tag="VI195_00220"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY6822834.1"
                     /translation="MRERNQIDWNVWLDSYKDTYASFSKAQHDGFRALEAFARFNYAV
                     AGDVLEAGFAQAKATLGARAAFGTQAIAELFEKQAELGTQLSEKIRERAQEF"
     gene            555..1016
                     /locus_tag="VI195_00225"
     CDS             555..1016
                     /locus_tag="VI195_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY6822835.1"
                     /translation="MADTMPLSDLGKHVGEEMGVSSWITLDQARITEFAHCTADHQWI
                     HVDSERAARESPFGTTIAHGFLTLALIAPTAFEILVARVAPKSVVNYGLEKVRFHAPV
                     RAGKRVRNRIRLAAVEDRGHGRYLVTTDNTIEIEGEIRPALQASALAIFMA"
     gene            complement(1139..3550)
                     /locus_tag="VI195_00230"
     CDS             complement(1139..3550)
                     /locus_tag="VI195_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6822836.1"
                     /translation="MKSEARVVVIGGGVVGTSVLYHLTKAGWKDLLLIERGELTCGST
                     WHAAGGMHTLNGDPNVARLQQYTINLYKELERISGQSCGLHMPGGIMLAGTRERFDWL
                     KMAQARGRYLGMELELISAEEAAKVFPIIDKKYFVGALLDPIEGHIDPYGVTHAYAKA
                     AQIGGAEIVRHNRVLDLKARPDGTWDVITEGGNVRAEHVVNAAGLWAREVGRMVGLEL
                     PVLAMQHQYLITEDLPALKGQKELLTAIDFEGEIYTRQERGGVLLGTYERAGVPWSPT
                     ETPWDFTQSLLPEDLERIAPSLEVGFRHFPTLAEVGIRKVVNGPFTFAPDGNPLVGPI
                     RGLRNFWVACGVMAGFSQGGGVGLTLSQWMTEGDPGADVWAMDVARYGDWATLAYTNV
                     KVRENYSRRFRIRFPNEELPAARPLRTTPVYERLQAAHAVLGEYCGLEHALWFAPSAA
                     EAHEDITFHRSNAHPHVAAECSAVHESMGLLETSNYGKFEVTGPRAEEWLAYVMANRV
                     PQVGRIALTPMLNERGKLIGDFTMCRLAEQRIFLICTWAAEVYYRRWFERHPPPAGVT
                     VRPCAMEYVGLSLAGPRSRELLQGLTRTDLSSAAFPFLTFRRMDIGMVPALVGRISFT
                     GELGYEIWVTSEYQRALYDLLCAAGRPHGLRLFGGRALNVMRIEKSFGTWAREYRPIY
                     GPHEAGLGRFVDFAKGEFIGRAAALEERQSGGALRLVTFSVDAADADAAGDEPIWHDG
                     RVVGWVTSGAYGHRTRRSLALGYIPAALSAATDGFEIELIGERRRAHRESAAVLDPAG
                     SRMRV"
     gene            complement(3569..4531)
                     /locus_tag="VI195_00235"
     CDS             complement(3569..4531)
                     /locus_tag="VI195_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6822837.1"
                     /translation="MQKKTIDRRWLPLNALRAFEGVAKHGSFTAAANALLISQSALSR
                     HVIALERLTGVQLFERRPHALTLTKAGQHLLPAVVKSFDRLEYALDDIRNEGAPAQRT
                     LRVQMPPSFAVQLAVPILRDFHRICAEVDIDLVSPHGVGPPLGDVDVAVIYSRPTVTD
                     LVTDLLWSERLSILCAPGIAATRRGKDLAAFIEANEIVHIRLADQPRHFLWLQFVRQA
                     GLSVDVERGLVVDTAVLAAQYALRGQGLALADPNLFAEELSSGSLVRPFDAALDAGYG
                     YYLTTPPEELGDSAIALFRSWLIERFGRRSPTSQPPVQLAVSNE"
     gene            complement(4679..>6772)
                     /locus_tag="VI195_00240"
     CDS             complement(4679..>6772)
                     /locus_tag="VI195_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6822838.1"
                     /translation="LAALESASHALPEDAEVQYLLAQALEEAGLLGRAAASLARATAL
                     RPDFADAHNDLGRLLLELGDTDAAITSCQRALALRPRFAAALGNLANAERARGALASA
                     IDGYRRAIALEPGLAEAHGNLGSALIETGEPEAGIDALRRALELRPRYAAAVTQLARA
                     LTARGRAAEALPYYERLLAHERRADTLNDYGTLLAGLSRFEAAADCFRAAHDLAPEEA
                     RIHANLGHVLHCLGDHRAAIGHSRRAIELEPRLAEGHLHLGNALLAINEMHAAEAAYR
                     AGLEVFPQHAALHTAHAMAERALGRVDDAAASVRRSLAIHPDAADTVALLGSLAIDRG
                     RFEEAETLLNKALTLSPGLPEALIGLTAVRKMTAADSAWRDAAERTLSRALPVAHAIG
                     LHHALGKYCDDVGDFDAAFEHHRRGHELARRSRPPYDRAATTARVNRTLTAFDGSALE
                     ALRPAGLTSERPIFVFGMPRSGTTLAEQILASHPRVHGAGEVLFWQFAADAELALPPE
                     ERMAAIAALGRQYLASFAALPAAAARVIDKLPSNFKNLGLIHAALPTARFIHLERHPL
                     DTCLSIYFQGFSAAHAYATDLSDLAHYYREYRRLMAHWRATLPPDLLLEVRYESLVDD
                     PEHWSRRMLAHLGLSWDPRCLEFHHTERPVLTASSWQVRQPIGRGAIGRWRHYERHLE
                     PLRAALGDDALERNG"
BASE COUNT         1135 a         2214 c         2322 g         1101 t
ORIGIN      
        1 gaactcctgg gcgcgctcgc ggatcttctc gctcagctgc gtgccgagct cggcctgctt
       61 ctcgaagagt tcggcgatgg cctgggtgcc gaaggctgcc cgggcgccga gcgtcgcctt
      121 tgcctgggcg aagccggcct cgagcacgtc gccggcgacc gcgtagttga agcgcgcaaa
      181 agcctcgagg gcacgaaagc cgtcatgctg cgccttgctg aaggaagcgt acgtgtcctt
      241 gtatgaatcc agccacacat tccagtcgat ctgattgcgt tcccgcatgt gagctcctca
      301 gggagaaacc tgtcgtttag gggggtcttg cagcacggaa ttatactggg atttgtatgc
      361 actgcaagat aaaaaaaatc acgtgcgagt tgtctaacta tatattgatt tataagtata
      421 aaaattaaca tttaagcttc tgtctagcac aagtatcgtg cagtgcaatg aaccgttgag
      481 ctgccggtgg gcgccttccg ccacagcccg gctcaggccg cttggtaaga tccggatcaa
      541 gaagcgaggg agccatggcg gacacgatgc ctttgagcga tctgggcaag cacgtcggtg
      601 aggagatggg cgtctcttcc tggatcacgc tcgatcaggc gcgcattacc gagtttgccc
      661 actgcacggc cgatcatcag tggatccatg tcgacagcga gcgcgcggcg cgcgagagtc
      721 ccttcggcac gacgatcgcg catggctttc tgacgctcgc actgattgcc cctacggcgt
      781 tcgagattct cgtggcgcgc gtggcgccga agtcggtggt caactacggg ctcgagaaag
      841 tgcgcttcca cgctccggtg cgcgcgggca agcgcgtacg caaccgcatc cggctcgcgg
      901 cggtggaaga tcgcggccat ggccgttatc tcgttaccac cgacaacacc atcgagatcg
      961 aaggcgagat cagacccgcg ctgcaggcga gcgcgctcgc gattttcatg gcgtgacggc
     1021 ggaccgggtg cgcggcccac gcttacagct cttttgaccc acagttaacc agttggtgaa
     1081 gtattgcggc cgagtgtggt agtgctgcgg gctgtgcagc gagcgctcga ttgagctctc
     1141 aaacgcgcat acgggacccg gcggggtcga gcacggctgc gctctcacga tgggcacgcc
     1201 gccgctcgcc gatcagctcg atctcgaaac catcggtagc ggcgctgagc gccgcgggga
     1261 tgtagccgag cgcgagcgag cggcgcgtgc gatgtccgta ggcaccggag gtcacccagc
     1321 cgacgacccg gccgtcgtgc cagatgggct catcgcccgc ggcatcggca tcggcggcat
     1381 cgacgctgaa ggtcacgagg cgcaacgcgc cgccactctg ccgctcctcg agtgccgcgg
     1441 cacgcccgat gaactcgccc ttggcgaaat cgacgaagcg cccgaggccg gcctcatgag
     1501 gaccatagat cggccggtac tcacgcgccc aggtcccgaa gctcttctcg atacgcatga
     1561 cgttcagcgc gcgccccccg aagagcctca aaccatgcgg acgtcccgcc gcacagagca
     1621 ggtcgtacag cgcgcgctgg tactcgctcg tcacccagat ctcgtagccg agctctccgg
     1681 tgaaggagat gcgcccgacg agtgccggga ccatgccgat gtccatgcgg cggaaggtga
     1741 ggaacgggaa ggcggcgctc gacagatcgg tgcgcgtgag gccctgcagc agctcgcgcg
     1801 accgcggacc tgcgagcgaa aggcctacat actccatggc gcacggacgc accgtgacac
     1861 cggcgggcgg cggatgacgc tcgaaccaac gccggtaata gacttccgcg gcccaggtgc
     1921 agatgagaaa gatgcgctgc tcggcgaggc ggcacatggt gaaatcaccg atgagcttgc
     1981 cgcgctcgtt cagcatcggg gtgagcgcga tgcgcccaac ctgcggcacc cggtttgcca
     2041 tgacgtacgc gagccactcc tcggcgcggg gacctgtgac ctcgaacttg ccgtagttgg
     2101 aagtctcgag gaggcccatg ctctcgtgca ccgcgctgca ttcggcggcg acgtgcgggt
     2161 gggcgttgga gcgatggaag gtgatgtctt cgtgcgcctc ggctgccgag ggcgcgaacc
     2221 acagcgcatg ctcgagcccg cagtactcgc cgagcaccgc atgcgcggcc tgcaggcgct
     2281 cgtacacggg cgtcgtccgc agcggacgtg cggccggcag ctcctcgttc gggaaacgga
     2341 tgcggaagcg gcgtgagtag ttctcgcgca ccttgacgtt ggtgtaggcg agtgtcgccc
     2401 agtcgccgta gcgcgcgaca tccatcgccc acacatcggc tccgggatcg ccctccgtca
     2461 tccattgcga gagcgtgagc ccgactccgc caccctggct gaagcccgcc atgacgccgc
     2521 aggcaaccca gaagttcctg agcccacgga tcggcccgac gagtggattg ccgtccggtg
     2581 cgaaggtgaa gggtccgttc acgaccttgc ggatgcccac ctcggcgagt gtcggaaaat
     2641 ggcggaagcc gacctcgagg ctcggcgcaa tgcgctcgag gtcctcgggc agcaggctct
     2701 gcgtgaagtc ccagggcgtc tcggtaggtg accacggcac accggcgcgc tcgtaagtgc
     2761 cgagcagcac gccaccgcgt tcctggcggg tgtagatctc gccctcgaag tcgatggcgg
     2821 tcagcaactc cttttgtccc ttgagtgcgg gcagatcctc ggtgatgagg tactggtgct
     2881 gcatggcgag cacgggcagc tcgagaccca ccatgcgccc cacctcgcgc gcccagaggc
     2941 ccgcggcatt caccacgtgc tcggcgcgca cgttgccacc ctcggtgatc acatcccagg
     3001 tgccgtcggg gcgcgccttg agatcgagca cgcggttgtg gcgcacgatc tctgccccgc
     3061 cgatctgcgc ggccttggcg taggcgtgcg tcacgccgta ggggtcgata tgaccttcga
     3121 tcggatcgag cagtgcaccg acaaagtact tcttgtcgat gatcggaaag accttggccg
     3181 cctcttcggc ggagatgagc tcgagctcca tgccgaggta gcggccgcgt gcctgcgcca
     3241 tcttcagcca gtcgaagcgc tcgcgcgtgc cggcgagcat gattccaccc ggcatgtgca
     3301 ggccgcagga ctggccggag atgcgctcga gctccttgta gaggttgatg gtgtactgct
     3361 gcagtcgtgc aacgttggga tcaccgttga gcgtgtgcat gccgccggcc gcatgccagg
     3421 tcgagccgca ggtgagctcg ccgcgctcga tcagcagcag atccttccag cccgccttgg
     3481 tgaggtgata gagaacactg gtccccacca ccccgccgcc gatcacgact acgcgtgcct
     3541 cactcttcac gtcccctccc ccggccgatc actcgttcga taccgccagc tgcactggcg
     3601 gctgactcgt ggggctacgc ctgccgaagc gctcgatgag ccatgagcgg aacagcgcaa
     3661 tggccgagtc gccgagctcc tccggcgggg tcgtcaggta atagccgtag cccgcatcga
     3721 gcgctgcatc gaagggtctc accaggctgc cgctgctcaa ctcctcggcg aacaggttag
     3781 ggtccgcgag cgcaaggccc tgtccacgca gcgcgtactg cgcggccagc accgcggtat
     3841 cgacgaccag tccgcgctcg acgtcgacgc tcaggcccgc ctggcgcaca aactgcagcc
     3901 acaggaagtg ccgcggctga tcggccagcc tgatgtgcac gatctcgttg gcttcgatga
     3961 aggccgccag atccttgccg cggcgcgtgg cggcgatacc gggcgcgcac aggatcgaca
     4021 gccgttccga ccacagcagg tcggtgacca gatccgtcac ggtcgggcgc gagtagatca
     4081 cggcgacatc gacatccccg agcggcggcc ccacaccgtg cggactgacg aggtcgatgt
     4141 cgacctccgc gcaaatgcga tggaagtcgc gcaggatcgg caccgcgagc tgtaccgcga
     4201 agctcggcgg catctgcacg cgcagcgtgc gctgtgccgg ggcgccttca ttgcggatgt
     4261 catcgagtgc gtactcgagc cgatcgaagg acttcaccac cgcaggcagc aggtgctggc
     4321 cggccttggt gagcgtgagg gcgtgcgggc ggcgctcgaa gagctgcacc ccggtgaggc
     4381 gctcgagcgc tatcacgtgg cgtgacaggg cgctctgcga gatgagcagc gcgttggcgg
     4441 cggccgtgaa gctgccgtgt ttggcgaccc cctcgaaagc gcgcagcgca ttcagcggca
     4501 gccagcgacg gtcgatggtt tttttctgca cgtggtgtcc ccgagtcaaa gcccccgcgc
     4561 tgcagtcatc ttgtcggccg cgccgtgggc gccgtgctca tactcgcaga gccgccagcg
     4621 tcgcgccaag agcccgccgg gctccaatgc cttttttcga taccgtccgc aacggcccct
     4681 agccattgcg ctcgagggca tcgtcgccga gcgccgcgcg cagcggctcc agatggcgct
     4741 cgtagtggcg ccaccgcccg atcgcgcctc tgccgatggg ctgccgcacc tgccagctgc
     4801 tcgccgtcaa cacgggtctc tcggtatggt ggaattccag acagcgcgga tcccaggaca
     4861 ggccgaggtg cgcgagcatc cgccgactcc agtgctcagg gtcatcgacc agggactcgt
     4921 agcggacctc gagcagcagg tccgggggaa gcgttgcacg ccagtgcgcc atcagccgcc
     4981 ggtattcacg gtagtagtgc gcgagatcgc tgagatccgt ggcatacgcg tgggcggcag
     5041 agaaaccctg gaaatagatc gacaggcagg tgtcgagcgg atggcgttcg aggtggatga
     5101 agcgcgccgt ggggagcgcc gcgtggatga ggccgaggtt cttgaagttg ctcgggagct
     5161 tgtcgatcac ccgcgccgct gcggcgggca gcgccgcgaa cgacgccaga tactgacggc
     5221 cgagcgcggc aatcgccgcc atgcgttcct ccgggggcaa ggcgagctcg gcatcggccg
     5281 cgaattgcca gaagaggacc tcgcccgccc catgcacccg tgggtgtgag gccaggatct
     5341 gctcggcgag cgtagtaccc gagcgcggca tcccgaagac gaaaattggc cgctcggacg
     5401 tgagtcccgc tggccgcagc gcctcgagcg ccgatccatc gaatgccgtc aacgtgcggt
     5461 tgacgcgagc cgtagttgcc gcgcgatcgt agggcggccg gctgcggcgg gcgagctcgt
     5521 ggcctcggcg gtgatgctcg aaggccgcat cgaagtcgcc gacgtcgtcg cagtacttgc
     5581 cgagagcgtg gtggagcccg atggcgtgtg cgaccggcag cgcgcgtgag agcgtgcgct
     5641 cggccgcgtc ccgccaggct gaatcagcgg cggtcatctt gcggaccgcg gtcagaccga
     5701 tcagtgcctc gggcagcccg ggactcagcg tcagcgcctt attgagtagc gtctcggcct
     5761 cctcgaaccg cccgcggtcg atcgcgaggc tgccgagcag ggcaacggta tcggcggcgt
     5821 cgggatgaat ggcgagcgag cgccgcacgc ttgcggccgc gtcatcgact cgtccgaggg
     5881 cccgctcggc catcgcgtgg gcggtgtgaa gcgccgcatg ctgcggaaag acctcgagtc
     5941 cggcgcggta ggcggcctcg gcggcgtgca tttcgttgat cgcgagcagc gcattgccga
     6001 gatgcaggtg cccttccgca agccgcggct cgagctcgat tgcgcgccgg ctgtgcccga
     6061 tggcggcgcg atgatcgccg aggcagtgga gcacgtggcc gaggttggcg tggatgcgcg
     6121 cctcttccgg agccagatcg tgtgccgcgc ggaagcagtc tgccgctgct tcgaaccggg
     6181 agagcccggc aagcagtgtc ccgtagtcgt tcagcgtatc cgcgcgccgc tcgtgtgcga
     6241 gcaaccgctc gtagtagggc agcgcttcag cggcacggcc gcgggcggtc aatgcccgtg
     6301 caagctgcgt aaccgcggcc gcgtaacgag gccgcagctc cagcgcgcgc cgcaaggcgt
     6361 cgatgccggc ctccggctca ccggtctcga tgagcgcgct accgaggttc ccgtgcgcct
     6421 ccgcgagtcc gggctcgagc gcaatggcgc ggcggtagcc atcgatcgcg gatgcaagcg
     6481 caccccgcgc gcgctcggcg ttggcaagat tgccgagcgc tgcggcaaac cgcggccgca
     6541 gcgcgagcgc gcgctgacag ctcgtgatgg cggcgtcggt gtcgccgagc tccagcagta
     6601 agcggccgag gtcgttgtgc gcgtcggcga aatccggccg cagagccgta gcacgggcga
     6661 ggctcgcggc cgcgcggccg agcaggcccg cctcttcgag cgcctgcgcc aagaggtact
     6721 gcacctcggc atcctccggc agggcatggc tcgccgattc gagcgcggcg ag
//
LOCUS       DATDMP010000015         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_668411, whole genome shotgun sequence.
ACCESSION   DATDMP010000015 DATDMP010000000
VERSION     DATDMP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668411"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..749
                     /gene="dps"
                     /locus_tag="VI195_00245"
                     /gene_synonym="pexB"
     CDS             267..749
                     /gene="dps"
                     /locus_tag="VI195_00245"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEY6822839.1"
                     /translation="MFDTRNDLPAKVRVEVTELLNARLADAIDLGAQSKHAHWNVKGP
                     NFIALHELFDKVAESIEEQIDTLAERVTALGGRARGTIASVARHSTLKPYPEDISEGL
                     AHVDALSTALADYGAKLRAAIETADRLGDADSADLFTGISRETDKYLWFLEAHLHAQR
                     "
     gene            765..1886
                     /locus_tag="VI195_00250"
     CDS             765..1886
                     /locus_tag="VI195_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6822840.1"
                     /translation="MSVEVRRTATGLQLELAGQWGLPEVEAARAQLAAVDLGAVHAVE
                     LSTRGMKSLDLSGAWVLREFLQRARAAGAEVSFQGTPPDQLRLIDQTLKEPEPGSQAV
                     VAAPAARVGLLYDIGRYAVHAWHDAVLSFAFLGRTLVTFFRSLRSLRRLRPISVARHV
                     YDTGITAMPITALIAFLVTVIIAYLSAQELRGLGVDIYVVDLVTIGVLRELGVLLTSI
                     ITAGRSASSYAAELGSMQLNEEVDALVATGVDPFEVLVVPRMLGFVIALPLLTVVADL
                     VGLTGGALLCRYLLDMPLTQYVNRVDEAIAPTTFWVGIVKAPVFAVLIALAGCYRGMQ
                     VRGSARELGHKVTVAVVQAIFLVILADALFAIVFMKMDI"
     gene            1889..2680
                     /locus_tag="VI195_00255"
     CDS             1889..2680
                     /locus_tag="VI195_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6822841.1"
                     /translation="MRYPVIEVRGLVNRFGSQLVHDGLDMEVRQDEVFGIVGGSGSGK
                     SVLLRSILGLQHPEAGTVRIEGHDITRLSEVQLRPIKARYGVAFQSGALYSGLTVLQN
                     VQLPMMEYLELESAVRDELALLKIRLVGLPVDAAGKYPAELSGGMVKRAAIARALALD
                     PALLFLDEPTSGLDPVSATAFDELVLFLQKELKLTIVMITHDLDSIFRTCNRVGVIVN
                     RRMESDTVERIVDNPNPWIQEYFHGERARGARLAALEGAGSDHGA"
     gene            2670..>2987
                     /locus_tag="VI195_00260"
     CDS             2670..>2987
                     /locus_tag="VI195_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY6822842.1"
                     /translation="MEREANYAAVGAFVLVVLLLGALFIYWYSDTREHRDYNRYEIYF
                     TGSVSGLDKGGAVRYLGVSVGRVVDLRVDPRDSSRVEVIVDIDARTPISTHTVAELQL
                     QGIT"
BASE COUNT          469 a          973 c         1028 g          517 t
ORIGIN      
        1 acccacctca tcagcaagca cgaagtgcgc gccgtcggga catcgctcga agtgctcaat
       61 ttccgcggcg tgatgacgag catcgacctg tggctcggac tcgcggcgac cgcggcattg
      121 gtgtatgcgg cagtccgcat ccgccgctac cgcgacgaca gctaacagca ccccatagat
      181 cgcgcttatg atcctgatcc ggacaagcga ttggccggcg cgcgcgcgtg gcggtctaat
      241 ggggctccgc agcagaggag actgacatgt tcgatacccg caacgacctg cccgccaaag
      301 tgcgcgtgga ggtgactgag ctgctgaacg cacggctcgc ggacgccatc gacctcggtg
      361 cccagagcaa gcacgcccac tggaacgtca agggcccgaa tttcatcgcg ctgcacgagc
      421 tgttcgacaa ggtcgccgag agcatcgagg agcagatcga caccctcgcc gagcgcgtca
      481 ccgcactcgg ggggcgcgca cgcggcacca tcgccagcgt ggcgcgccac agcacgctca
      541 agccgtaccc cgaggacata tccgagggac tcgcgcacgt ggatgccctg tcgaccgcgc
      601 tcgcggacta cggcgcgaag ctgcgcgctg cgatcgagac ggccgaccgg ctcggggacg
      661 ccgacagcgc ggacctcttc accggcatct cccgcgagac cgacaagtat ctgtggttcc
      721 tcgaggcgca cctgcacgcc cagcgctgag gcgacgcccg ccgcatgtcc gtcgaggttc
      781 gccgtactgc aaccgggctg cagctggagc tggcaggcca gtggggactg cccgaggtgg
      841 aggctgcccg ggcgcaactc gcggcggtcg acctcggcgc tgtgcacgcg gtggagctca
      901 gtacccgcgg catgaagtcg ctcgacctct cgggcgcatg ggtgctgcgt gaattcctgc
      961 agcgcgcccg cgcggcaggc gccgaggtgt ccttccaggg cacgccaccc gatcagctgc
     1021 gactgatcga tcagacgctg aaggagccgg agcccggcag ccaggccgtg gtggcggcgc
     1081 ctgccgcccg ggtcggcctg ctgtacgaca tcggccgcta cgcagtacac gcctggcacg
     1141 atgcggtgct gtcgttcgcc tttctgggcc gcacactggt cactttcttc cgtagcctgc
     1201 gcagcctgcg gcggttgcgg ccgatctccg tggcacggca cgtgtacgac accggcatca
     1261 ctgccatgcc gatcacggcg ctgatcgcct ttctcgttac cgtgatcatc gcttacctga
     1321 gcgcgcagga gctgcgcgga ctcggggtgg atatctacgt ggtggacctg gtgaccatcg
     1381 gcgtgctgcg tgagctcggc gtgctgctca cctcgatcat cacggccggg cgttcggcga
     1441 gcagctacgc agccgagctc ggctcgatgc agctcaacga ggaggtggac gcactggtcg
     1501 cgaccggcgt cgacccgttc gaggtgctgg tggtgccacg gatgctcgga ttcgtgatcg
     1561 cgctgccgct gctcacggtg gttgcggacc tggtcggcct gaccggcggg gccttgctgt
     1621 gccgctattt gctcgacatg cccctcaccc agtacgtcaa ccgggtcgat gaggccatcg
     1681 cgcccaccac tttctgggtc ggcatcgtca aggcgccggt gttcgcggtg ctcatcgcgc
     1741 tcgccggctg ctatcgcggc atgcaggtac gcggctcggc gcgcgagctc gggcacaagg
     1801 tgacggtcgc ggtggtgcag gcgatctttc tggtgatcct cgccgatgcg ctgtttgcga
     1861 tcgtgttcat gaagatggac atctgaggat gcgctacccg gtgatcgaag tgcgcgggct
     1921 ggtcaaccgc ttcggcagcc agctggtgca cgatgggctc gacatggaag tccggcagga
     1981 cgaggtgttc ggcatcgtcg gcggttcggg ctcgggcaaa tcggtgctgc tgcggagcat
     2041 cctcggcctg cagcatccgg aggccggcac ggtgcgcatc gagggccatg acatcacgcg
     2101 cctgagcgag gtgcagctgc gcccgatcaa ggcgcgctac ggggtggcct tccagagcgg
     2161 cgcgctgtac tcggggctta ccgtgctgca gaacgtgcag ttgcccatga tggagtatct
     2221 cgagctcgag agcgcggtgc gcgacgagct ggcgctgctc aagatccgcc tggtcggcct
     2281 gccggtggat gccgcgggca agtaccccgc ggagctctcc ggcggcatgg tgaagcgcgc
     2341 cgccatcgcc cgtgctctgg cgctcgatcc ggcgctgttg tttctcgacg agccgacctc
     2401 cggtctcgat ccggtgagcg ccacggcgtt cgacgagctg gtgttgttcc tgcagaagga
     2461 gctgaagctc acgatcgtca tgatcacaca cgatctggac agcatcttcc gcacctgcaa
     2521 tcgtgtcggg gtgatcgtga accggcgcat ggaatcggac acggtggagc gcatcgtcga
     2581 taacccgaac ccctggatcc aggagtattt ccatggcgag cgggcgcgcg gcgcccgcct
     2641 cgccgcgctc gagggcgccg ggagtgatca tggagcgtga agccaactac gctgcggtcg
     2701 gggcgttcgt gctggtcgtg ctgctgctcg gcgcgctgtt catctactgg tattccgaca
     2761 cgcgcgagca ccgcgactac aatcgttacg agatttactt caccggcagc gtgagcgggc
     2821 tcgacaaggg tggggcggtg cgttacctgg gtgtgagcgt cggccgggtg gtcgacctgc
     2881 gggtcgaccc acgtgactcc tcgcgggtgg aggtgatcgt cgacatcgat gcacgcaccc
     2941 cgatctccac gcacaccgtg gcggagctgc agctgcaggg catcacc
//
LOCUS       DATDMP010000016         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1001902, whole genome shotgun sequence.
ACCESSION   DATDMP010000016 DATDMP010000000
VERSION     DATDMP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001902"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VI195_00265"
     CDS             complement(<1..620)
                     /locus_tag="VI195_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY6822843.1"
                     /translation="MSYAEDPLPQENQPETRTSVRYLEVAPEDAGQRLDKFVQQHLRD
                     VPRSRVYRVIRKGEVRVNGRRAGPATRLEADDKVRLPPVRLQPLRPPGSPAAGLLARI
                     AAAVIHEDERLLVIDKPAGIAVHGGSGVSNGVIEALRALRPAESLELVHRLDRETSGC
                     LLVARQAATLRSLHALLREGALEKRYLALLKGRWQLGTKRIEAALRT"
     gene            979..>2306
                     /locus_tag="VI195_00270"
     CDS             979..>2306
                     /locus_tag="VI195_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY6822844.1"
                     /translation="MKRMLVNATQEEELRVALVDGQKLFDLSIELPSREQKKANIYKA
                     RISRIEPSLEACFVDYGAQRHGFLPLKEVSREYFRQQPQGGRMNIRELLAEGQELIVQ
                     VEKEERGTKGAALTTFISLAGRFLVLMPNNPRAGGVSRRIEGEERDQMREVMGQLQIP
                     DGMGAIVRTAGVGRAVAELQWDLDNLRVQWEQIAAAAKDRTAPFLVFRESDAVTRAMR
                     DYLSDDVGEVLVDSQAAFDKAQEYMQRFMPAEAQRRLKLYTDDIPLFTRFQIESQIES
                     AYAHKVQLPSGGSIVIDYTEALVSIDINSARATRGSDIETTATNTNLEAADEIARQLR
                     IRDIGGLIVIDFIDMESSKNQRDVEDRLRDAMKMDRARIQIGRLSRFGLLEMSRQRLR
                     PSLGETSHIVCPRCVGIGSIRSIESMTLAVLRLIGEELRKDRTTRVITQVP"
BASE COUNT          390 a          782 c          767 g          367 t
ORIGIN      
        1 gtgcgcagcg ccgcctcgat gcgcttggtg ccgagctgcc accgtccctt gagcagcgcg
       61 agataacgtt tctcgagcgc tccctcgcgc agcagtgcgt gcaggctgcg cagcgtcgcc
      121 gcctgacgcg caaccagcag acacccgctc gtctcgcggt cgaggcggtg cacgagctcg
      181 agcgattccg cgggacgcag ggcgcgcagc gcctcgatca ccccgttgct cacgccgctg
      241 ccgccgtgca cggcgatccc cgccggcttg tcgattacga gcagccgctc gtcctcgtgg
      301 atgacggccg cggcgatgcg ggcgaggagc ccggcggccg ggctgccggg cggccgcaag
      361 ggctgcagcc gcaccggcgg gagccgcacc ttgtcgtcgg cctcgaggcg cgtcgcggga
      421 ccggcgcgcc gtccgttgac gcgcacctcg cccttgcgga tgacgcggta gacccggctg
      481 cgcggcacgt cacgcaggtg ctgctggacg aacttgtcga gccgctggcc ggcatcctca
      541 ggagccacct cgaggtagcg cacggacgtg cgcgtttccg gctggttctc ctgcggcaag
      601 gggtcctctg cgtaagacat tgtttctaca acactctctg aagtatactc tcgcggtcgt
      661 ccgtgaaacg gacgcatcaa aaggcttgcg gccacgggtc gcagcctctt tagttggacc
      721 tctttaaacc gaggccatcg cattgggccg acaagatcgc gccgtctgcg tcgaatctcc
      781 gaagcgaggt tccgcagtgg gcgtcgtcgg ttgagtcacc gaaggtttcc tgtgcgcccc
      841 gccccgctgt ggcgggcacg aacatgaaca acgagagtca ctctcccttg tgtcctagga
      901 atcttcgccg tcgcggaccg ctcggtccgc ggcctcccgt cgagcgcttg ccggcccctc
      961 aacccacagt aggggcacat gaagagaatg ctggtgaatg caactcagga agaggagttg
     1021 cgcgtcgctc tggttgacgg acagaagctg ttcgacctga gtatcgagtt accttcccgc
     1081 gagcagaaaa aggccaatat ctacaaggcg cgtatctcgc gcatcgagcc ctccctcgaa
     1141 gcctgcttcg tcgattacgg cgcacagcgc cacggcttcc tgccgctgaa ggaagtctcc
     1201 cgcgagtact tccgccagca gccgcagggc gggcggatga acatccgcga gctgctcgcg
     1261 gagggccagg agctcatcgt ccaggtggag aaagaggagc gcggcaccaa gggtgcggcg
     1321 ctcaccacct tcatcagcct cgccgggcgc tttctggtgc tgatgccgaa caatccgcgc
     1381 gccggcggcg tctcgcgccg catcgagggc gaggaacgcg accagatgcg cgaggtgatg
     1441 ggccagctgc agatcccgga tggcatgggt gccatcgtgc gcaccgccgg cgtcggccgc
     1501 gccgtggcgg agctgcagtg ggacctcgac aacctgcggg tgcagtggga gcagatcgcc
     1561 gcggccgcca aggaccgcac cgcgcccttc ctggtgttcc gcgagagcga tgccgttacg
     1621 cgcgccatgc gcgactacct gtccgacgac gtcggggagg tgctggtgga cagccaggcg
     1681 gcgttcgaca aggcccagga gtacatgcag cgcttcatgc cggccgaagc gcagcggcgc
     1741 ctgaagctct acaccgacga catcccgctg ttcacccgct tccagatcga gagccagatc
     1801 gagtcggcct acgcccacaa ggtgcagctg ccctccggcg gcagcatcgt gatcgactat
     1861 accgaggcgc tggtgtcgat cgacatcaat tcggcgcgcg ccacccgcgg cagcgacatc
     1921 gagaccaccg cgaccaacac caatctcgag gccgcggacg agatcgcacg ccagctgcgc
     1981 atccgtgaca tcggcggact catcgtcatc gacttcatcg acatggagtc gagcaagaac
     2041 cagcgcgacg tcgaggaccg gctgcgcgac gccatgaaga tggatcgtgc ccgcatccag
     2101 atcgggcggc tgtcgcgctt cgggctgctg gagatgtcgc gccagcggct gcggccctcg
     2161 ctcggcgaga cgagtcacat cgtctgcccg cgctgcgtcg gcatcggcag catccgcagc
     2221 atcgagtcga tgacgctcgc cgtgctgcgc ctgatcggtg aggagttgcg caaggaccgg
     2281 accacgcgcg tgatcacgca ggtgcc
//
LOCUS       DATDMP010000017         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1001934, whole genome shotgun sequence.
ACCESSION   DATDMP010000017 DATDMP010000000
VERSION     DATDMP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001934"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1600
                     /gene="tldD"
                     /locus_tag="VI195_00275"
     CDS             146..1600
                     /gene="tldD"
                     /locus_tag="VI195_00275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY6822845.1"
                     /translation="MSDSVAADPLDLARDLILRPSGLDDRRLDGVFGEVLTHSVDFAD
                     LYFQLTHQESWSLEDGMVKDGSASIEQGVGVRALAGEKTGFAYSDEIVLPALTEASRA
                     ARAIAVHGGARRVAAWHARNGHRLYLPADPAADLHTPEKVAWLERVDREARRIDPRVV
                     HVSASLVAVHEVVLIATSDGELAADVRPLVRFNVSALVEQDGRREQGYVGAGGRFSLA
                     ELVAGERPLALAREAVRQALINLEARPAPAGTMTVVLGPGWPGILLHEAIGHGLEGDF
                     NRKGTSAFANRIGERVAAAECTVVDDGTLARRRGSLNIDDEGTPTQCTMLIENGVLRG
                     YMQDRLNARLMGVPPTGNGRRESFAHMTLPRMTNTYMRAGPHAPEEIIRSVERGIYAV
                     NFGGGQVDITSGKFVFSASEAYAIENGRIGTPLKGATLIGNGPDVLTRVSMVGNDLKL
                     DDGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            1902..2069
                     /locus_tag="VI195_00280"
     CDS             1902..2069
                     /locus_tag="VI195_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822846.1"
                     /translation="MLSEEVTVEVQKLQLPPELLAEVARRNNGKRQPDFRVEGLKPSL
                     FERLARVLGLG"
     gene            2141..2395
                     /locus_tag="VI195_00285"
     CDS             2141..2395
                     /locus_tag="VI195_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822847.1"
                     /translation="MPRLSPHISRPLYEGLPWIYIILGLVALIASYLLAARGALSLIV
                     GVLGLGCVVGGFVLLLRRRDYRALRSQYADPESLSGHDKR"
     gene            complement(2437..>3197)
                     /locus_tag="VI195_00290"
     CDS             complement(2437..>3197)
                     /locus_tag="VI195_00290"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6822848.1"
                     /translation="TETETQSYIQHRLDVAGAGDQELFAADTFAEIYRYTGGVPRLVN
                     TLCDTAMMAAYTADRGVVTREDIGNAIAELQWSEYAVRPHRYRHEEPAAAAPTPAARA
                     AAAHAGARVLGRLLVAADGRTVQEVPLRLGRLIIGRTPDNDVQIDSRFISRHHCQVIT
                     TLNSCVVEDLNSTNGIYVKSNRVRRHYLNDGDVVLVGKHELIYVDERAGRHRGALTDN
                     LPALERTVEGAAREAQLEEVEAADDEDLEATGPR"
BASE COUNT          494 a         1063 c         1102 g          538 t
ORIGIN      
        1 tattggggac ggatactgca gcgccggccg cgcggctgcg gctgcgcgct ggcggaaatc
       61 gatcttgccg cgcaggcgag cgtgcgcgag agctttccgg cgctcgcaca ccgcgccttt
      121 gcaccgcacg gcacaggaaa gcaacatgag tgacagcgtg gctgccgatc cgctcgatct
      181 cgcgcgcgat ctcattctgc ggccgagcgg tctcgacgat cgccgtctgg atggcgtgtt
      241 cggcgaggtg ctcactcact cggtggactt tgccgacctg tactttcagt tgacccacca
      301 ggagtcctgg tcgctggaag acggcatggt gaaggacggc agcgccagta tcgagcaggg
      361 cgtcggtgtg cgtgcgctcg ccggcgagaa aaccggcttc gcctattcgg acgagatagt
      421 gctgccggcg ctcaccgagg catcgcgcgc ggcgcgcgcc atcgccgtgc acggcggcgc
      481 gcggcgcgtc gccgcctggc acgcgcgcaa cggccatcgc ctgtacctgc cggccgatcc
      541 tgcggccgat ctgcacactc ccgagaaggt ggcatggctc gagagggtcg atcgcgaggc
      601 gcggcgcatc gatccgcgcg tggtgcacgt cagcgccagc ctggtcgcgg tgcacgaagt
      661 ggtgttgatt gccaccagcg acggtgagct tgccgcggat gtgcggccgc tggtgcgctt
      721 caacgtctcc gcgctggtcg agcaggatgg acggcgcgag cagggctacg tgggcgccgg
      781 cggccgtttc tccctcgccg agctggtcgc gggtgagcgt ccgcttgcgc tggcgcgtga
      841 agccgtacgt caggcgctca tcaacctcga agcgcggccg gcgcccgcgg gcacgatgac
      901 ggtggtgctg ggtccgggct ggccgggaat actgctgcac gaggcgatcg gccacgggct
      961 cgaaggcgac ttcaatcgca agggtacctc cgcgttcgcc aaccgcatcg gcgagcgggt
     1021 cgcggccgcc gaatgcacgg tggtcgatga cggcacgctc gcgcggcggc gcggctcgct
     1081 caacatcgac gacgaaggca ccccgaccca gtgcaccatg ctcatcgaga acggtgtgct
     1141 gcgcggttac atgcaggacc gcttgaacgc gcgcctcatg ggcgtgccgc ccaccggcaa
     1201 cggccggcgc gagtcgtttg cgcacatgac gctgccgcgt atgaccaaca cctacatgcg
     1261 cgcgggccca cacgctcccg aggaaatcat ccgctcggtg gagcgcggca tctacgccgt
     1321 caatttcggc ggcggccagg tggacataac ctcgggcaag ttcgtgtttt ccgcgagcga
     1381 ggcgtatgcc atcgagaacg gccgcatcgg cacgccgctg aagggcgcga cactgatcgg
     1441 caatgggccg gatgtgctca cgcgcgtctc gatggtcggc aacgatctga agctcgacga
     1501 tggggtcggc acctgcggca aggaaggcca gagcgttccc gtgggtgtcg gacagcctac
     1561 gctccggatt gatgggctga cggtcggcgg taccggctga gacaccgcgg gtcgttgcgc
     1621 gcgccgccag cggcggcgac cgcgtggcaa gcgcaaccgg ggcagtaaca aactcaatct
     1681 cccgggaaca ccggcgtcgc gcgcgcaagc gcacacttca cagcgccgct cgaaggcagc
     1741 gctgtcgccg gatacgcacg tcgcgccgcg gcaaccctgg tcgtcagtcc gcgacataat
     1801 gacgaacgct ggcgaaggtg tgattcttct ctcgcacggc aagcccgcga ccccctaacg
     1861 tagatcgggc gcactttgag tcttacgcgg aaggaaatgc gatgctgtca gaagaggtca
     1921 ctgtcgaggt tcagaagctg caactgccac ccgagctgct ggcggaagtt gcccgccgca
     1981 acaatggtaa gcgccaaccg gacttccgcg tcgaaggttt gaagccctca ctgttcgagc
     2041 gcctggcgcg ggtgttgggt ctcggctgat tccaactcag gcggcgatca ctgccgagtg
     2101 ttgagcgaaa tttcctgggg tgctttagga tggggatgcc atgccgcgcc tcagtccgca
     2161 catctcccgt ccgctgtacg aaggattgcc ctggatttac ataatcctcg ggttggttgc
     2221 gctcatcgcc agctatctgc tcgccgcacg cggcgcgctc agcctgatcg tgggcgtgct
     2281 cggcctcggc tgcgtcgtgg gaggtttcgt actgctgctg cgccggcgtg actaccgcgc
     2341 gctgcgctcg caatacgccg atcccgagtc cctgagcggc cacgacaagc gctgaccgcc
     2401 gcgcggcccc acgccggcgc gctccatcac gctggctcag cgcgggccgg tagcctccag
     2461 gtcctcgtcg tccgctgcct cgacttcctc gagctgcgcc tcacgcgcgg ctccctccac
     2521 cgtccgctcg agcgccggca gattgtcggt gagtgcgcca cggtgccgcc cggcgcgctc
     2581 atcgacatag atcagctcgt gcttgccgac caggaccaca tctccgtcgt tgagatagtg
     2641 gcggcgcacg cggttggatt tcacgtagat gccgttggtg ctgttgaggt cctcgacgac
     2701 gcacgagttg agcgtggtga tcacctggca gtggtggcgg ctgatgaagc gactgtcgat
     2761 ctgcacgtcg ttgtcggggg tgcggccgat gatcagccgc ccgagccgca acggcacttc
     2821 ctgtaccgtg cggccatccg ccgcgaccag cagccgaccc agcacccgcg caccggcatg
     2881 cgcggccgca gcgcgcgctg caggcgtggg cgcggcggct gccggctcct catgccggta
     2941 gcggtgcggc cgcaccgcgt actcgctcca ctgcagttcc gcgatcgcgt tgccgatgtc
     3001 ctcgcgcgtc accacgccgc gatcggcggt gtaggcggcc atcatggcgg tatcgcacag
     3061 ggtgttgacc agccgcggca cgccgccggt gtagcggtag atctccgcga aggtgtcggc
     3121 agcgaacagc tcctgatcgc cggcgccggc cacatccagg cgatgctgga tgtagctctg
     3181 ggtctcggtc tcggtga
//
LOCUS       DATDMP010000018         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_557804, whole genome shotgun sequence.
ACCESSION   DATDMP010000018 DATDMP010000000
VERSION     DATDMP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557804"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VI195_00295"
     CDS             complement(<1..705)
                     /locus_tag="VI195_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6822849.1"
                     /translation="MTGNAASLAQALALQQQGHLAEAAALCRQVLAREPHNSDAMHLL
                     GLAAATVGDVPQALALLGAAVQLQPGNAAMHANLASALAQAGRHLAAVGCYDRALALQ
                     PQLAIAHRGRGTALMHAGRLDEAVTSFLQALRLAPEDDRAHNGLGVTLVRAGRPAEAR
                     QHLERAVTLNPGSVDAHHNLALLESAAGRHREALASIERALALQPENPELHTQRGVEM
                     LALAQPLEAIVSFQRVL"
     gene            complement(702..>2274)
                     /locus_tag="VI195_00300"
     CDS             complement(702..>2274)
                     /locus_tag="VI195_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6822850.1"
                     /translation="NRTEAAAAHLRRRLQQAPRDVVALRLLADAATRLEDDVEAERRL
                     NECLELAPGYAQARYDLARLLNEYQRFDEMMPHVERLLACDPDNIDCLNLKAQGLRFI
                     GRIDEAIALMEQLLVDHPDEEQSWVLCGSLLRTLGESDRAIEMFRRALVVRAGSSLAY
                     SSLANLKTFRFEKADLEAMEHQLASPAVRGVDRIRLEFALGKGLEDAGEFATSFEHYA
                     RANRLQRTTFFYDPQIMTADVQRVRTFFTRSFFAERSGWGSRRSDPIFIVGLPRSGST
                     LLEQILASHSLVEGTRELLDLPTVAFDLISPVADRNESTFLQRVAALRPEEVESLAMQ
                     YLSRTQLSRQTGKARFIDKLLGNWIYLGFIHLLFPRATIIDTRRHPLGCGFSCYRQQF
                     GRGQKFTYDLAEFGRFYRDYAAVMEHFDATLPGRVHRVYYEQLVADPRGELQRLLEHC
                     GLPFEEQCLRFYENRRAVTTISSEQVRRPIYGDSVDQWRHYEAWLAPLKEVVGELVAR
                     YPAPRPAASETPRQP"
BASE COUNT          400 a          729 c          760 g          385 t
ORIGIN      
        1 gagcacgcgc tggaagctga caatcgcctc cagcggttgc gcgagcgcga gcatctcgac
       61 accccgctgc gtgtgcaact ccgggttctc cggttgcagc gcgagggcac gctcgatgct
      121 cgccagcgcc tcgcggtgcc gcccggccgc cgattccagc agggcgagat tgtggtgggc
      181 atcgacgctg cccggattga gcgtcactgc acgctcgagg tgctgccgtg cttccgcagg
      241 ccgtccggca cggaccagcg tcacgcccag accgttgtgc gcgcgatcat cctcgggtgc
      301 gagccgtaac gcctgcagga aactcgttac cgcctcatcg agccttcctg cgtgcattag
      361 cgcggtgcca cggccgcggt gcgcgatggc tagctgcggc tgcagggcga gcgcgcggtc
      421 ataacagccg accgccgcga ggtggcgtcc ggcctgggcg agcgcgctag cgaggttggc
      481 gtgcatcgcg gcgttgcccg gctggagctg gacggccgca ccgagcagcg ccagcgcctg
      541 cgggacatca cccaccgtcg cggcagcgag ccccagcagg tgcatggcgt cgctgttgtg
      601 tggctcacgt gcaagcactt gacgacacag agcagcggcc tcggccagat gcccctgctg
      661 ctgcaacgca agggcctgcg cgaggctggc cgcgttgccg gtcatggctg cctgggagtc
      721 tcggaggctg ccgggcgggg cgcggggtag cgcgccacca gctcgcccac gacctccttc
      781 aacggtgcca gccacgcctc gtaatgacgc cactggtcga cgctgtcgcc gtagatcggc
      841 cggcgtacct gctcggagct gatcgtggtc acggcgcgtc gattctcgta aaagcgcagg
      901 cactgttctt cgaagggcag cccgcagtgc tcgaggagcc tctgcaactc gccccgcgga
      961 tcggcgacga gctgctcgta gtaaacgcga tgcacgcgac cggggagcgt cgcgtcgaaa
     1021 tgctccatca ccgccgcgta atcgcgatag aaccgtccga actccgcgag atcgtaggtg
     1081 aacttctggc cgcggccgaa ttgctgccgg tagcacgaaa aaccgcagcc gagcggatgg
     1141 cggcgtgtat cgatgatcgt cgcgcgcgga aacaacaggt ggatgaatcc aagatagatc
     1201 cagttaccga gcagcttgtc gatgaagcgc gctttgccgg tctgacggct gagttgcgtg
     1261 cgcgagaggt actgcattgc cagtgactcg acttcctcgg ggcgcagcgc tgcgacccgc
     1321 tgcagaaacg tcgactcgtt gcgatcagcg accggggata tcagatcgaa tgccactgta
     1381 gggaggtcca gcagctcgcg cgtgccttca accagcgagt ggctcgccag gatctgttcg
     1441 aggagcgtcg atccggagcg tggcaggcct acgatgaaga tcggatcgga tcgccggctc
     1501 ccccatccgg aacgctccgc gaagaagctc ctggtgaaga aggtccgtac gcgctgcaca
     1561 tccgcggtca tgatctgcgg atcgtagaag aaagtggtgc gctgcaggcg gttggcgcgc
     1621 gcatagtgtt cgaaggaggt cgcaaactca cccgcatcct cgagcccctt gccgagcgcg
     1681 aactcgagcc gtatgcggtc aacgccacgt accgccgggg atgcgagctg gtgctccatc
     1741 gcttcaaggt cagccttttc gaagcgaaag gtcttgagat tggcgaggct ggaataggca
     1801 agactcgatc cggctcgtac gacgagtgcg cggcgaaaca tctcgatggc tcgatccgat
     1861 tccccgagcg tacgcagcag actgccacag aggacccagg attgttcttc atcgggatga
     1921 tccacgagca gctgttccat cagcgctatc gcctcgtcga tccggccaat gaagcgtaat
     1981 ccctgcgcct tgaggttcag gcaatcgatg ttatccggat cacatgcgag cagccgctcg
     2041 acgtgcggca tcatctcatc gaatcgctga tactcattga gcaggcgcgc caggtcatag
     2101 cgtgcctgcg catagccggg cgcgagctcg aggcactcgt tgagacgccg ctcggcctcc
     2161 acatcgtcct cgagcctggt ggctgcatcg gcgagcagcc gcagcgccac gacatcgcga
     2221 ggggcctgtt gcagacgccg ccgcaagtgc gcggcggcgg cctcggtccg gttg
//
LOCUS       DATDMP010000019         8202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_781017, whole genome shotgun sequence.
ACCESSION   DATDMP010000019 DATDMP010000000
VERSION     DATDMP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781017"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /gene="ybeY"
                     /locus_tag="VI195_00305"
     CDS             <1..244
                     /gene="ybeY"
                     /locus_tag="VI195_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY6822851.1"
                     /translation="YQDPRRRVAGGDIVLCHPVIRREARAQRKALAAHYAHLVVHGML
                     HLRGYDHHQKGAALLMEKAEIRALRRFGLRNPYELK"
     gene            255..1079
                     /locus_tag="VI195_00310"
     CDS             255..1079
                     /locus_tag="VI195_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY6822852.1"
                     /translation="MNDPARPSLLERLSALIMREPGDREQLLELLHAAYSRNLLDADA
                     LSIIEGALTVSEMQVRDIMIPRAQVDFIDLHDPVESFIPRVISTAHSRFPVIDQNRDD
                     VIGVLLAKDLLRHYAGEEEFNVREMLRPAVFVPEAKRLNVLLREFRASRNHMAIVVDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDEASDNILPEAGGRFRVKALTQIADFNAALG
                     TQFPDETADTVGGLVIAHLGRLPKRGETVLIDGLRFQVLRADSRRVYTLLVDKPKT"
     gene            1076..2539
                     /gene="lnt"
                     /locus_tag="VI195_00315"
     CDS             1076..2539
                     /gene="lnt"
                     /locus_tag="VI195_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY6822853.1"
                     /translation="MRALHYAAAFVAGAAAVLSFAPAGVYPVALVSFAVLIHLWMRAS
                     PAACLGLGACFGLGLFGAGVSWVYVSLSEFGGMPPVLAVVATLLFCGVLACFTGAAGW
                     LQARIPASPFVRATLLSPSSWALFEWLRSWLFTGFPWLSAGYAASGWSMEGFAPVLGV
                     FGISWLTFFIAGCIWSMANRERRLAAVALSVSVLLAGEGLRAVVWTAPTGAPVTVALL
                     QGNIEQDLKFRPERYEHILQTYAALAEGSSTQLIVLPETAIPRFLDRIDPAYLARLGA
                     VAQRNGGDLLLGVPYRAPGNRYYNSVITLGHAPQQAYHKVHLVPFGEYMPTGFHWTLR
                     LLDIPLSDFSSGSKDQRALSVADQRVAVNICYEDAFGAEIAARARDATLLVNVSNVAW
                     FGDSLAPGQHLQIARMRALETGRMHVAATNTGITAAIDRDGRVLARLPQFTAGRLEIA
                     ARGYAGTTPYMRFYDWPVIIASLLVLGAAVGFARRGR"
     gene            2554..3444
                     /gene="glyQ"
                     /locus_tag="VI195_00320"
     CDS             2554..3444
                     /gene="glyQ"
                     /locus_tag="VI195_00320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY6822854.1"
                     /translation="MLTFQQLILQLNAFWDRHGCVLLQPYDMEVGAGTFHTATFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPSPADIQDLYLRSLQALGI
                     DPREHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQEVGSLPCRPVLGEITY
                     GLERLAMYLQGKESVFDLAWVDGVTYGDVYHQNEVEQSRYNFELSDPELLFRHFAEYE
                     AQAKRLMEAQCVLPAYEMVLKCSHTFNLLDARGAISVTERAAYIGRVRALARAVAQSY
                     YESRERLGFPMLSADTAASQ"
     gene            3441..5489
                     /gene="glyS"
                     /locus_tag="VI195_00325"
     CDS             3441..5489
                     /gene="glyS"
                     /locus_tag="VI195_00325"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY6822855.1"
                     /translation="MTETLLVELLTEELPPKSLRAVSEAFGRSLATELERDGFVAQAA
                     ARTFATPRRLAVQIADVAARSPDAQREVQGPPVSAQAQAVSGFARKNGVPVEQLERRP
                     TPKGEVYVALVRTPGRPLAEVLAEKVQAALKTLPIPKLMRWGSGDAQFIRPVHGVVLL
                     HGERAVPGTVLGVASGGTTRGHRFLANHAIPLANAQDYEAALVREGKVLPDLSARQAE
                     IERQLIERAAAEGASLGAYQDLLEEVAALVELPAVYVGTFDPKFLQVPQECLILTMRQ
                     NQKYFPLFDADGTLQPKFLIVSNMPVRDPSAIITGNERVVRPRLEDARFFYDQDRKQP
                     LESRVPALAKVVYHNKLGTQLERVQRIQLLAGTIARKLGADPMLAERAAWLSKADLLT
                     GMVGEFPELQGVMGRYYARHDGEPQAVADAIEAHYRPRFSGDRLPQGSVACTVALADK
                     LDGLAGLFGIGQAPTGDKDPFALRRAALGLVRILAEQRLAYPLHDLVEAAVKNYAGKS
                     GFDNTRAEVENFIYERARHYFAETGYSPPEVEAVLCLRPSRLDLVPKQLEAVRAFSAM
                     PEAESLAAANKRIANILKQAGAAPAGFDSELLTLKEEKDLAALFGRIKPQVERAFDGL
                     DYAAGLCALASLKAPVDAFFDKVMVMDKDPRVRSNRIGFLGALHGTMNRIADLSKLAK
                     "
     gene            5486..6019
                     /gene="gmhB"
                     /locus_tag="VI195_00330"
     CDS             5486..6019
                     /gene="gmhB"
                     /locus_tag="VI195_00330"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY6822856.1"
                     /translation="MKLIILDRDGTINHDSDQYIKSTAEFRTLKGSVEAIARLTQAGY
                     RIVVATNQSGISRGLFDTATLISMHDMLQRAVSQAGGRIDAFFFCPHAADSGCECRKP
                     KPGMLLEIARRFNVSLADVYMVGDAPRDIEAAAAAGARPVLVLTGKGRKTREDGGLPP
                     GTAVFPDLAAFAEHLAP"
     gene            6079..6735
                     /locus_tag="VI195_00335"
     CDS             6079..6735
                     /locus_tag="VI195_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6822857.1"
                     /translation="MLALATLPLSRMARYHVISGWSRLVVQLARRILGITWRVEGRER
                     LPARPSVILAKHQSAWETLAFQVIFPPQVHVLKRELLWIPFFGWGLALMSPIAIDRMR
                     GMAALRTMARKGRERLAQGFWIVVFPEGTRVGPGERRPYQPGGAWLASACNAPVVPVA
                     HNAGLFWPRNAFLKRPGTVIVRIGPTIDPEGRDPQTINALAEEWIEAQQKTLYPPSSS
                     "
     gene            6687..7421
                     /locus_tag="VI195_00340"
     CDS             6687..7421
                     /locus_tag="VI195_00340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY6822858.1"
                     /translation="MDRSPAENPVPAVIKLGGRLIPYRFERRRRRTLGITVDVEGLSV
                     AAPLRASWRDIESFLHDRERWILSKLDQWSRLPRAPMLTGASGELLPYLGRSLYLDVR
                     QSEGRAVRHHFDRLVISAPGPRRALETLVGWLKSKALEALVPRTEHFAALLGVPAPSV
                     GLSSARGQWGVCTEGGNIRLNWRLVHVDPSLIDYVVAHEVAHLMEMNHSKRFWSLLSG
                     LYPDWRPARERLEIAGAALPIIGGKR"
     gene            7418..7804
                     /gene="gloA"
                     /locus_tag="VI195_00345"
     CDS             7418..7804
                     /gene="gloA"
                     /locus_tag="VI195_00345"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY6822859.1"
                     /translation="MRILHTMLRVGDLKRATGFYTEVLGMKLLRTTDRPEQKYSLAFV
                     GYGPEDSTAVLELTYNYGVERYDLGSAYGHIAIEVEDIRKSCDLVRSRGGKVTREPGP
                     VKGGTSVIAFVEDPDGYKIEFIERKR"
     gene            complement(7785..>8202)
                     /locus_tag="VI195_00350"
     CDS             complement(7785..>8202)
                     /locus_tag="VI195_00350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEY6822860.1"
                     /translation="ISTPLLFHLAAFAGSVRDMHFMLQEEVVARMVAAPSTPEYGRLS
                     VSLQARFAMRKLFTVARGAFRPPPRVESAVVQLQPLQNILSVDEDLLRRAFSARRKTL
                     RNALPEVDFAAAGIDSGLRPENLSPADYAKLSACAR"
BASE COUNT         1363 a         2744 c         2639 g         1456 t
ORIGIN      
        1 ctatcaggac ccgcggcgcc gtgttgccgg gggcgacatc gtgctgtgcc acccggtcat
       61 ccggcgggaa gcgcgcgcgc agcgcaaggc gctcgcggcg cactatgcgc acctggtggt
      121 gcacggcatg ctgcacctgc gcggctacga tcatcatcaa aaaggcgccg ccctgctcat
      181 ggagaaggcc gaaatccgcg cgctgcggcg cttcggcctg cgtaatcctt acgagttaaa
      241 atgatcgttc ccccatgaac gatcccgcgc gccccagcct gctcgagcgc ctttcggcgc
      301 tgatcatgcg cgagccgggc gatcgcgagc agctgctgga gctgctccac gcggcgtact
      361 cgcgcaacct cctcgacgcc gacgctcttt ccatcatcga gggtgcgctc acggtttcag
      421 agatgcaggt gcgcgacatc atgatccccc gcgcgcaggt ggacttcatc gatctgcacg
      481 atccggtcga gagcttcatt ccgcgcgtca tttccacggc gcattcccgc ttcccggtca
      541 tcgaccagaa ccgcgacgat gtcataggcg tgcttctcgc gaaggatctg ctgcgccact
      601 acgctggaga ggaagagttc aatgtgcgcg agatgctgcg cccggcggta ttcgtaccgg
      661 aggcgaagcg gctgaacgtg ctgctgcgcg aattccgcgc gagccgcaac catatggcca
      721 tcgtggtgga cgaatacggc ggcgtcgccg ggctcgtcac catcgaggac gtgctggagc
      781 agatcgtcgg cgacatcgag gacgagtacg acttcgatga ggcgtcggac aacatcctcc
      841 ccgaagcagg cgggcgcttc cgcgtcaaag cgctcacgca gatcgccgac ttcaatgccg
      901 cgctgggcac gcagtttccc gacgagaccg ccgacaccgt gggcggcctc gtcatcgcgc
      961 acctgggcag gcttcccaag cgcggcgaga cggtgctgat cgacggcctg cgtttccagg
     1021 tgctgcgggc cgacagccgg cgcgtttata ccctgctggt agacaagccg aagacgtgag
     1081 ggctctgcac tatgccgcgg ccttcgttgc aggcgcggct gcggtgctct cgtttgcgcc
     1141 ggccggcgtc tatccggtcg cactcgtctc cttcgcagtg ctcatccacc tctggatgcg
     1201 cgcctcgccg gccgcgtgcc tagggctcgg cgcgtgtttc ggtctgggcc tcttcggcgc
     1261 gggggtttcc tgggtctatg tgagcttgag cgagtttggc ggcatgccgc cggttcttgc
     1321 cgtggtagcg acgctcctct tctgcggcgt gctcgcgtgc ttcaccggcg cagccggatg
     1381 gcttcaggcc cggattcccg cttccccatt cgtgcgagcc acgctcctca gtccatcgag
     1441 ctgggcgttg ttcgagtggt tgcgctcctg gctcttcacc ggcttcccct ggctttccgc
     1501 gggctatgcg gcgagcggct ggtccatgga ggggttcgcg ccggtgctcg gcgtttttgg
     1561 catttcctgg cttaccttct tcattgccgg ctgcatttgg agtatggcga accgcgagcg
     1621 caggcttgca gccgtggcgt tgtccgtgtc cgtgctcctg gcgggggaag gcttgcgcgc
     1681 agtggtttgg acggcgccta cgggagcgcc cgtgaccgtc gcgctcctgc agggaaatat
     1741 cgagcaggac ctgaaattcc gtcccgagcg gtacgagcac atcctccaga cgtatgcggc
     1801 gctcgccgaa ggcagctcga cgcaactcat cgtgctgccg gaaaccgcga tcccgcgctt
     1861 tctcgaccgc atcgacccgg cgtatctcgc ccgcctgggc gcggtcgccc agcgcaacgg
     1921 cggcgacttg ctccttggag ttccatatcg agccccagga aaccggtact acaacagcgt
     1981 cattaccctc ggccatgcgc cgcaacaggc gtatcacaag gtgcacctgg tgcccttcgg
     2041 tgaatacatg cccaccgggt ttcactggac tttgcgcctc ctggacattc cgctttcgga
     2101 cttctcctcg ggcagcaagg accagcgtgc gctttcggtc gcggaccagc gggtcgccgt
     2161 caacatctgc tacgaggacg cattcggagc cgagatcgcg gcgcgtgcac gcgatgccac
     2221 attgctcgtc aacgtttcga acgtcgcgtg gttcggcgac tcgctcgcac ctggccagca
     2281 tcttcagatc gcccgcatgc gggccctgga gaccgggcgc atgcacgttg ccgcgaccaa
     2341 taccgggatt actgccgcca tcgaccgcga tggccgcgtc ctggcgcggc tgccgcagtt
     2401 cacggccgga aggctcgaga tcgcggcgcg gggctatgcc ggcacgacgc cctacatgcg
     2461 cttctacgac tggccggtga tcatcgcctc gttgctggtg cttggcgcag cggtagggtt
     2521 cgcgcggcgc ggccggtaga atccgctcct ctgatgctta ccttccagca gctcatcctg
     2581 cagctcaatg ctttctggga ccgccacggc tgcgtgctcc tgcaacccta cgacatggaa
     2641 gtaggcgcag ggactttcca taccgcgacg ttcctgcgcg cgatcggccc cgaaccctgg
     2701 cgtgccgcgt acgtgcagcc gtccaggcgc ccgaaagacg gacgctatgg cgacaatccg
     2761 aaccggctgc agcactacta ccagtaccag gtcgtgctaa agccctcgcc tgccgacatc
     2821 caggatcttt atctccggtc gctgcaggcc ctcggcatcg accctcgcga gcacgacatc
     2881 cggttcgtcg aggacgattg ggaatcgccg acgctcggcg cctggggact gggatgggaa
     2941 gtctggctgg atggaatgga ggtcacgcag tttacgtact ttcaggaagt cggaagcctc
     3001 ccctgccggc cggtgctggg cgagatcacc tacgggctcg agcgactggc catgtatctg
     3061 cagggcaagg aaagcgtgtt cgacctggcc tgggtcgacg gtgtcaccta cggcgacgtc
     3121 taccatcaga acgaagtcga gcagtcgcgc tacaacttcg agctttcgga tcccgagctc
     3181 ctttttcgcc acttcgcgga atacgaggcg caagccaagc ggctcatgga agcccagtgc
     3241 gtgctgccgg cgtacgagat ggtgttgaaa tgctcgcaca ctttcaacct gctcgatgcg
     3301 cgcggcgcca tctccgtcac cgagcgcgcc gcgtacatcg gaagagtgcg tgcacttgct
     3361 cgcgcagtcg cgcagagcta ttacgagtcg cgtgaacgcc tcggtttccc catgctatcg
     3421 gcggacacgg cggcctcgca atgaccgaga cgctcctcgt cgagctcctc accgaggagc
     3481 tgccgcccaa atcattgcgc gcagtctccg aagcgttcgg tcgcagcctc gccacggagc
     3541 tcgaacgcga cggcttcgtc gcacaagcgg ctgcgcgcac gtttgcgacg ccgcgccggc
     3601 tcgccgtcca gatcgcggat gtcgctgcgc gatcgcccga cgcgcagcgc gaagttcaag
     3661 ggccgccggt gagcgcgcag gcgcaagccg taagcgggtt cgcccgcaag aatggcgtcc
     3721 ccgtcgagca gctcgagcgc cggccaacgc ccaaggggga ggtctatgtc gccctcgtgc
     3781 gcacgccagg ccgtcctctc gcggaggtgc tcgcggaaaa ggtgcaggcg gcgttaaaga
     3841 cgctgcccat cccgaagctc atgcgctggg gcagtggcga cgcgcaattc attcgaccgg
     3901 tccatggcgt ggtgctcttg catggtgagc gcgcagtgcc cggaaccgtg cttggcgtcg
     3961 cgtccggtgg cacaacccgc gggcatcggt ttctggcgaa ccatgccata ccgctcgcca
     4021 atgcgcagga ctacgaagct gccttggtgc gcgagggcaa ggtgctgccc gatttaagcg
     4081 cgcgtcaggc ggaaatcgag cgtcagctca tcgaacgcgc cgccgcagaa ggggcaagcc
     4141 tcggcgccta ccaggacctg ctcgaggagg ttgcggcgct agtcgagctt ccggccgtct
     4201 acgtcggaac cttcgatccg aagtttctcc aggttccgca ggagtgcctg atcctcacga
     4261 tgcggcagaa ccagaaatat ttccccctgt tcgacgccga cggaacgctg cagccgaaat
     4321 ttctaatcgt ctccaacatg ccggtacgcg acccgagcgc gatcatcacc ggcaacgagc
     4381 gtgtcgttcg accgcgcctc gaagacgcgc ggttcttcta tgaccaggac cgcaagcagc
     4441 cgctggagtc gcgcgtcccg gcgctcgcca aggtcgtgta ccacaacaag ctcggcaccc
     4501 agctagagcg cgtacagcgc attcagctgc ttgccggcac gattgcgcgc aagctgggcg
     4561 ccgacccgat gctggcagag cgcgccgcct ggctctcgaa ggccgacctc ctcacgggca
     4621 tggtgggcga attccccgag ctgcagggcg tcatggggcg ctattacgcg cggcacgacg
     4681 gcgagccgca agccgttgcc gatgccatcg aagcgcatta ccgcccgcgt ttttccggag
     4741 accggctgcc ccaaggctcg gtggcatgca ccgtcgcgct ggccgacaag ctcgacgggc
     4801 tggcgggact tttcggcatc ggccaggcgc cgacggggga caaggatcct ttcgcgctgc
     4861 ggcgcgcggc gctcggcctc gtgaggatcc tcgccgagca gcggcttgcc tatccgctgc
     4921 acgatcttgt cgaagcggcc gtcaagaact acgccggaaa atccggcttc gataacacgc
     4981 gggccgaggt cgagaacttc atctacgagc gtgcccgcca ctactttgcc gaaaccggct
     5041 attcgccgcc cgaggtcgag gccgtgctct gcctgcggcc atcgcggctt gacctcgtgc
     5101 ccaagcagtt ggaggccgtg cgcgcgttct cggcgatgcc ggaagccgaa agcctggccg
     5161 cggccaacaa gcgcatcgcc aacatcctga agcaggcggg cgccgcgcct gcagggttcg
     5221 attccgagct tctgacgctg aaggaggaaa aggacctcgc cgcccttttc gggcgcatca
     5281 agccccaggt agagcgcgcg ttcgacgggc tcgactacgc ggccggcctg tgcgcgctcg
     5341 cctccctcaa ggcgccggtc gatgcgttct tcgacaaggt gatggtcatg gacaaggacc
     5401 cgcgcgtgcg ctcgaaccgg attgggttcc tcggcgcgct gcacggcacg atgaatcgca
     5461 tcgccgatct ttccaagctc gccaaatgaa gctcatcatc ctggaccgcg acggcacgat
     5521 caatcacgac agcgatcaat acatcaaatc caccgcggaa ttccgcaccc tcaagggcag
     5581 cgtggaggcg attgcgcggc tcacgcaagc gggttaccgg atcgtcgtgg ccaccaatca
     5641 atcgggcatc tcgcgcgggc ttttcgacac cgcgacgctg atctcgatgc acgacatgct
     5701 gcagcgggca gtctcgcagg ccggcggtcg catcgatgct ttcttcttct gcccccacgc
     5761 ggcggattcc ggctgcgaat gccgcaagcc gaagcccggg atgctgctcg agatcgcgag
     5821 gcgcttcaac gtatcgcttg cggacgtcta catggttggc gacgcaccgc gcgacatcga
     5881 ggccgccgcg gcggccggcg cgcgcccggt cctcgtgctg acgggaaaag gacgcaagac
     5941 ccgggaggat ggcggactgc cgcccggcac ggcagtcttt ccagacctcg cggcgttcgc
     6001 cgagcatctg gccccatgat cgtcgcgcgc tcgagcctgt tcgcgctcgc gctgctgctc
     6061 gttaccccgc cctacgccgt gctggcgctc gccacgctgc cattgtcccg catggcgcgc
     6121 taccacgtca tttccggctg gtccaggctg gtggtccagc tcgcgcgacg aatcctcggc
     6181 atcacctggc gcgtggaagg acgcgaacgg ctgcccgcgc ggccttcggt cattctcgcc
     6241 aagcaccaat cggcctggga aacgctcgcc ttccaggtga tatttccccc gcaagtccat
     6301 gtcctgaagc gcgagcttct ctggatcccg ttcttcggtt ggggactcgc cctgatgagt
     6361 cccatcgcca tcgaccgcat gcgcggcatg gcggccctgc gcaccatggc gcgcaagggt
     6421 cgcgagcgcc tcgcgcaagg cttctggatc gtggtcttcc cggaaggcac ccgcgttggt
     6481 ccgggcgagc ggcgcccgta ccagcccggc ggcgcatggc tggcaagcgc gtgcaatgcg
     6541 cccgtcgtgc cggtggcgca caacgccggc ttgttctggc cgcgcaacgc ttttttgaaa
     6601 cgccccggca cggttatcgt ccgtatcggt ccaaccatcg atcccgaggg ccgggatccg
     6661 caaacgatca acgcgctcgc tgaagaatgg atcgaagccc agcagaaaac cctgtacccg
     6721 ccgtcatcaa gctaggcggt cggctgattc cgtaccgttt cgagcgccgc cgccggcgga
     6781 cgctcggaat cacggttgac gtcgaaggct tgagcgtggc agctccgctg cgcgcgtcgt
     6841 ggcgcgacat cgaatccttt ctgcacgacc gggagcgctg gatcctgtcc aagctcgacc
     6901 agtggtcgcg gctgcctcgc gctccgatgc tgaccggggc gagcggcgaa ttgctgccgt
     6961 acctgggacg gtcgctctac ctcgacgtgc ggcaaagcga agggcgcgcg gtccggcatc
     7021 actttgatcg gctcgtcatc tccgcacccg ggccgcgtcg cgcgctggaa acgctggtcg
     7081 gctggctgaa atccaaggcg ctggaagcgc tggtgccccg gaccgagcac ttcgccgcgc
     7141 ttctaggcgt tcccgcgcca agcgtcggac tttccagcgc gcgcggccag tggggcgtgt
     7201 gcaccgaagg cggcaatatc cggctcaact ggcgcctggt gcacgtcgac ccgtcgctta
     7261 tcgattacgt ggtggcgcac gaagtcgcgc acctcatgga gatgaaccat tcgaaacgct
     7321 tctggagcct cctttcgggc ctgtatcctg actggcggcc ggcccgggag cggctggaga
     7381 tcgccggggc ggccctgccg atcataggag gcaagagatg agaatcctgc acaccatgct
     7441 gcgagtcggg gatctaaagc gcgcgacggg cttttacacc gaagttctcg ggatgaagct
     7501 tctgcgcacg accgaccggc ccgagcagaa atattcgctc gctttcgtgg gttacggtcc
     7561 ggaggacagc acggcggtgc tggagctcac ttacaactac ggcgtcgagc gctacgactt
     7621 gggcagcgct tacggccata ttgcgatcga ggtcgaagat atccgcaaga gctgcgacct
     7681 cgtgcgtagc cgcggcggta aagtgacccg cgagcccggt cccgtgaaag gcggcaccag
     7741 cgtgatcgcc ttcgtcgagg atcccgacgg ctacaagatc gaattcatcg agcgcaagcg
     7801 ctgagcttcg cgtaatcggc cggcgacagg ttctcgggcc gaaggcccga gtcgattcca
     7861 gcagcggcaa aatctacctc gggcagcgcg ttgcgcaacg tcttgcggcg cgctgaaaag
     7921 gcgcgacgca gcaaatcctc gtccaccgag aggatgttct gcaggggctg cagttggacc
     7981 acggcggatt cgacgcgcgg cggcggccgg aacgcgcccc gcgccacggt aaatagcttg
     8041 cgcatcgcaa agcgcgcctg aaggctgacc gagagcctgc cgtactccgg cgtcgagggc
     8101 gccgccacca tccgcgctac cacttcttcc tgcagcatga aatgcatgtc gcgcacgctt
     8161 cccgcgaagg cggcgagatg gaaaagcagc ggcgtggaaa tg
//
LOCUS       DATDMP010000020         4549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1003031, whole genome shotgun sequence.
ACCESSION   DATDMP010000020 DATDMP010000000
VERSION     DATDMP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003031"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="rodA"
                     /locus_tag="VI195_00355"
     CDS             complement(<1..1045)
                     /gene="rodA"
                     /locus_tag="VI195_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY6822861.1"
                     /translation="MIAGILALLGVMTIYSAGADWSRQLLWVALGAVVYAAAAWFDYR
                     RLLPLAPGIYAAIVLLLIAVHLVGHTALGARRWLSVAGFPLEPSELSKLALVVVLAAL
                     LSRAELLSWRNFGLALTLAAPAAVLILTQPDLGTTIVFAAIFAGMLFVGGARPTQLMS
                     LVAAAAITLPALPFLLHGYQRARLAIFLDPSSDPLGAGYNLLQARIAVGSGGFFGQGF
                     LHGLQGQLGFVPERATDFVFAIFAEQFGLLGSLLLIALFGSLLIRILGAAAVAPDRFG
                     RMLAAGVFAMVLVQVVENIGMNIGALPIAGIPLPLISYGGSATITTLAALGLVQSVVL
                     RRSTIAHRPVPFEP"
     gene            complement(1087..2946)
                     /gene="mrdA"
                     /locus_tag="VI195_00360"
     CDS             complement(1087..2946)
                     /gene="mrdA"
                     /locus_tag="VI195_00360"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY6822862.1"
                     /translation="MIVLAGALSRPRWSFRLVAAGVLAIVALSTLAVKLAQLQVTDGA
                     SLAAMGRANSVHRIVLEADRGIIYDRHGVPLVQNAPAWNLELMPAALPSTTGARNAEI
                     AELAGLTGMSPVALTLAIEKADPYGSVKVGPDLTEAQELALAERVPGLPGVSIARHAV
                     RTYVDATITGHVLGYVGPIDAGEWKALRPLGYQPDESVGKVGVEAGLEKWLRGTDGWA
                     DVIVDARGRSVRTVAEQAPVPGSSVYLTLDLTLQLATAASLAAGLQRDHKKAGASVVV
                     DPRSGEVLAMVSIPGYDTNLFTHGISQAAYKQLLDDPLRPLLNRSIAGQYAPGSTFKM
                     VTAIAGLQEGKITPSTILGCPSYLTYGGWVYHNWASYSLGSMNVMRALAVSCDTFFYQ
                     VAAMVGDVALARYARAFGFGQAKDLEMPGVQPGLVPDRIWKALACGVPDQNSDACRWT
                     LGDTVTFGIGQSALLTTPLNQAIYVAAIANGGRVLQPTLVHEVKDASGRVVLRQKTVV
                     IDTVPVSAGNLAAVREGMREMINRPYNENYWFRAAGVPADGGGKTGTAQWGGSGLNLP
                     THAWFVFFAPFVSPDVAMSIFVERGELSEVEAAPIAVQIMKFFNAHRSDIQAP"
     gene            complement(2943..3434)
                     /gene="mreD"
                     /locus_tag="VI195_00365"
     CDS             complement(2943..3434)
                     /gene="mreD"
                     /locus_tag="VI195_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY6822863.1"
                     /translation="MSRALLLVLLAMAALVQVTWASRLELYGAFPNLVLIAVVCIAWV
                     RGPRAGLAWACIGGLLLDLTAPGPLGPHALALVAGAYAVGFWVRNVKLSSLPQAALAA
                     AAASAIYSAILVGADDLLGLPVPPFRLVFNLAVAAAIYNAVLVTAALIALRGFMKPAP
                     ETA"
     gene            complement(3431..4189)
                     /gene="mreC"
                     /locus_tag="VI195_00370"
     CDS             complement(3431..4189)
                     /gene="mreC"
                     /locus_tag="VI195_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY6822864.1"
                     /translation="MLLLAMVSQQPWAAGARGFLKGALTPFESAMTAAGNSVAQLTGG
                     WGDSSGLRTENARLKAQNAELQKEVAQLQAQGLDNQALRQALDFERTYGHSMLAAQVV
                     GRGPDGFSITLEIDRGSSDGLRPGMVVVSGAGLVGRIIETGPHAAIVETLADPQSRVS
                     AFLSSSALEGTVAGGPQSLSMAINPRFGIAPADGEWAITSGVGGGYPRGLVIGLVTSV
                     THKDTATTDSASLLWVNDPTVLSVVLVIKDFTPA"
     gene            complement(4246..>4549)
                     /locus_tag="VI195_00375"
     CDS             complement(4246..>4549)
                     /locus_tag="VI195_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY6822865.1"
                     /translation="AHVREALSPVLIDIVRAVRSTLDATPSELLEDIMHRGIVVCGGG
                     ALLAGLDRLFAHETLVPVRIAADPLTCVVRGTGLVLEEIDTLNRVLLRTRRVRALR"
BASE COUNT          821 a         1567 c         1537 g          624 t
ORIGIN      
        1 agggttcgaa tggaacgggc cggtgggcga tggtcgagcg ccgcaggacc acggactgga
       61 ccaggcccag cgccgccaac gtggtgatgg tcgccgagcc gccatacgag atcagcggca
      121 gcgggatgcc ggcgatcggc agcgcgccga tgttcatgcc gatgttctcg accacctgga
      181 ccagcaccat cgcgaagacg cccgcggcca gcatcctgcc gaagcgatcg ggcgcgaccg
      241 cggcggcgcc gaggatcctg atcaacaggg agccgaagag tgcgatcaga aggaggctcc
      301 cgagcaggcc gaactgctcg gcgaagatcg cgaagacaaa gtccgtggcc cgctcgggca
      361 caaaccccag ctggccctgc aggccgtgca agaacccctg gccgaagaac ccgccggacc
      421 cgaccgcgat gcgcgcctgc agcaggttgt agccggcgcc cagcgggtcg ctgctcgggt
      481 ccagaaaaat cgcgagccgc gcgcgctgat acccgtggag cagaaacggc agcgcgggca
      541 acgtgatcgc tgccgcggcg acaagcgaca tgagctgcgt gggcctcgcg ccgccgacga
      601 acagcatgcc ggcaaagatc gccgcgaaga cgatggtggt gccgaggtcg ggctgcgtga
      661 ggatcagcac ggccgccggc gccgccagcg tcagcgccag gccgaagttg cgccagctca
      721 acaactcggc gcggctgagc aacgccgcga ggacgaccac cagcgccagc ttcgaaagct
      781 ccgacggctc gaggggaaac cccgccaccg agagccagcg acgcgcgccg agtgcggtat
      841 gccccaccag gtgcacggcg atgagcagca gcacgatcgc cgcgtagatg cccggcgcca
      901 gcggcagcaa ccggcgatag tcgaaccaag ccgccgcggc gtaaacgacg gcgcccagcg
      961 ccacccacag gagctggcgg ctccaatcgg cgccggcgct gtagatcgtc atcacgccca
     1021 gcagcgccag gatgccggcg atcaccggct gtacgcggtc caatcgcagc cacggcgata
     1081 ccgaggtcac ggcgcctgga tgtcggagcg gtgcgcgttg aagaacttca tgatctgcac
     1141 cgcgatcgga gccgcctcga cttcggaaag ctctccccgc tcgacgaaga tcgacatcgc
     1201 gacatcgggc gacacgaaag gcgcgaagaa gacgaaccat gcatgcgtcg gcaggttcag
     1261 gccgctgccg ccccactgcg ctgtgcccgt cttgccgcct ccgtcggcgg gcacgcccgc
     1321 ggcccggaac cagtagttct cgttgtaggg ccggttgatc atctcgcgca ttccctcacg
     1381 gaccgcagcc agattgcccg cagacaccgg gaccgtatcg atgaccactg tcttctgccg
     1441 cagcacgacg cggccggacg cgtctttcac ctcgtgcacc agcgttggct gcagcacgcg
     1501 cccgccattg gcgatggccg cgacatagat cgcctggttg agcggcgtgg tcagcagcgc
     1561 agactggccg atgccgaaag tgacggtgtc gccgagagtc cagcgacacg catcggagtt
     1621 ctgatcggga acgccgcacg cgagagcttt ccagatgcga tcgggaacca gaccgggctg
     1681 cacaccaggc atctcgaggt ccttggcctg accgaagcca aaagcgcgcg cgtaccgagc
     1741 cagcgccacg tcaccgacca tcgctgcgac ttgatagaag aaggtgtcgc acgagaccgc
     1801 tagcgccctc atcacgttca tcgatccgag gctgtagctc gcccagttgt gatagaccca
     1861 gccgccgtag gtcaggtagc tgggacagcc gaggatggtg ctcggcgtga tctttccttc
     1921 ctgcaagccg gcgatggccg tgaccatctt gaaagtcgac cccggcgcgt actggcccgc
     1981 gatggaacga ttcagcagcg gtcgcagcgg atcatcgaga agctgcttgt aagccgcctg
     2041 cgagatgccg tgcgtgaaga ggtttgtgtc gtaacccggg atgctcacca tcgccagcac
     2101 ctcgccgctg cgcggatcga cgaccaccga cgcgccggcc ttcttgtggt cgcgctgcag
     2161 gcccgccgcc agcgaagcgg cggtcgcgag ctgcagcgtc aggtccagag tcaggtacac
     2221 cgagctgccg ggcaccggcg cctgctcggc gacggtgcgc accgaccggc cgcgagcgtc
     2281 gacgatcaca tcggcccatc cgtccgtgcc gcgcagccac ttctcgaggc ccgcttcgac
     2341 gccgaccttg cccactgatt cgtccggctg atatccgagc gggcgaagcg ccttccactc
     2401 gccggcgtcg atcggcccga cgtaaccgag gacgtggccg gtgatcgtgg cgtcgacgta
     2461 ggtccgcacg gcgtggcgcg cgatgctcac gccgggcagg ccgggcaccc gctccgcgag
     2521 cgccagctct tgggcttccg tgaggtcggg gccgaccttc acgctgccgt acgggtcggc
     2581 cttttcaatg gcgagggtca gcgccaccgg cgacatacct gtgaggccgg cgagctcggc
     2641 gatctctgcg ttgcgagcgc cggtggtcga cggcagggcc gccggcatga gctcgagatt
     2701 ccacgcgggt gcgttctgca cgagcggcac gccgtggcgg tcgtagatga tgccgcgatc
     2761 cgcctcgagc actatgcggt gcacggagtt cgcgcgaccc atcgccgcga ggcttgcacc
     2821 atcggtgacc tgcaactgcg cgagtttgac cgcgagcgtg ctgagggcga cgatggccag
     2881 cactccggcc gcgaccaggc ggaacgacca acgcggccgc gaaagcgcac cggccagaac
     2941 tatcacgcgg tctccggcgc aggtttcatg aaacctcgca gcgcgatcag cgccgccgtc
     3001 acgagcaccg cgttgtagat ggccgcggcc acggccaggt tgaacaccag gcggaatggc
     3061 ggtacgggaa gaccgagcag gtcgtcggcg ccgaccagga tcgccgagta gatcgccgat
     3121 gcagcagccg ctgcgagcgc cgcctgcggc aggcttgaaa gcttcacgtt gcggacccag
     3181 aacccgacgg catatgcacc tgcgacgagc gcgagagcgt gcggcccgag cggaccgggt
     3241 gcagtgaggt cgagcagcag gccgccgata catgcccacg cgaggcccgc gcgcggaccg
     3301 cgcacccacg cgatgcagac gaccgcaatc agaacgaggt tgggaaaagc gccgtacagc
     3361 tcgagccgcg acgcccacgt cacctgcacc agcgccgcca tcgcgagcag cacgaggagc
     3421 agggcgcggc tcatgccggc gtgaagtcct tgatgaccag gaccacgctg agcacggtcg
     3481 ggtcgttgac ccagagcagg ctggcgctgt cggtggtcgc agtgtcttta tgcgtgaccg
     3541 aggtcacgag gccgatgacc aggcctcgtg gatagccacc accgacgccg ctcgtgatcg
     3601 cccattcgcc gtcggcaggc gcgatgccga agcgcgggtt gatggccatg gacagcgact
     3661 gcgggccgcc ggcgacggtg ccttcgagcg ccgagctgga cagaaacgcg ctgacgcggc
     3721 tctgcggatc ggcgagcgtc tcgacgatcg cggcgtgagg tccagtctca atgattcgcc
     3781 cgacgaggcc ggcgccgctg accacgacca tcccaggacg caggccgtcg ctcgagccgc
     3841 ggtcgatttc gagcgtgatg ctgaacccgt cggggcctct gccgacgacc tgcgccgcca
     3901 gcatcgagtg accgtatgtg cgctcgaagt cgagagcctg ccgcaacgcc tggttgtcga
     3961 gcccctgcgc ctgcagctgc gcgacctcct tctgcaactc cgcgttctga gccttcagcc
     4021 gcgcgttctc agtgcgcaag ccgctgctgt cgccccagcc accggtgagc tgcgccacgc
     4081 tgttgcccgc agcggtcatc gctgattcga acggcgtcag cgcgcctttg agaaagccgc
     4141 gcgctcctgc ggcccacggc tgctggctga ccatcgccag cagcagcaac ccggcgcaga
     4201 gagtgagaaa cagacgggtc ggtcgcggtc gcttgctacc tgcgatcaac gcaaggcgcg
     4261 cacgcgacgg gtgcgcagca gcactcggtt cagcgtgtcg atctcttcca gtaccaggcc
     4321 cgtcccgcgc acgacgcacg tcagcgggtc ggcggcgatg cgcaccggca ccagcgtctc
     4381 gtgcgcgaaa agacggtcga gccccgcaag cagcgcaccg ccgccgcaca cgacgatgcc
     4441 gcgatgcatg atgtcctcga gcaattccga aggcgttgcg tcaagcgtgg atcggaccgc
     4501 acgcacgata tcgatcagca ccggcgacag cgcctcgcgg acgtgcgcc
//
LOCUS       DATDMP010000021         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1336407, whole genome shotgun sequence.
ACCESSION   DATDMP010000021 DATDMP010000000
VERSION     DATDMP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336407"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VI195_00380"
     CDS             <1..554
                     /locus_tag="VI195_00380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY6822866.1"
                     /translation="RRDIELSELGARQAHATARRIAAQCEPTAIYSSPLRRCLQTAAA
                     IGAACGGLATTVLEDLNDLHYGDWEWHTHEEVRARWPELLQRWFASPQLVRFPRGESL
                     QDLVARMANVLRRVRETHADETVVVVGHSSGNRALLLQTLDQPLSAYWRLAQDACSVS
                     DIELHPHAATLRRFNEIYWGSSS"
     gene            551..1537
                     /locus_tag="VI195_00385"
     CDS             551..1537
                     /locus_tag="VI195_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY6822867.1"
                     /translation="MSPASVSAPAPSDASGALGRRLTAFDIEAHRGGRARRPENTLPA
                     FADALSMGVDTLELDMGVTRDGVVVVSHERGLNPDLTRTPDGDYIAAEIPFVDLTLAQ
                     VKQFDVGQIRPGSAYAARFPDQRAIPGTRIPTLAEVFALVRRSGDRHVRLNIETKIDP
                     HRPAEALDPQHFVTAVLELLRRERFNDRVLIQSFDWRTLQLIQQQAAEVPTVYLTEQS
                     GPTATVHADAASLWTAGFDPRDHGGSLPRTIKAAGGSIWSPDYNDVDARSIEEAHRLG
                     LPVIVWTVNQPADMARLIDIGVDGIISDRPDLLRAEAAKKGIPLPPPAPVSP"
     gene            complement(1607..1771)
                     /locus_tag="VI195_00390"
     CDS             complement(1607..1771)
                     /locus_tag="VI195_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822868.1"
                     /translation="MRVTRRRYRSSIWLQRIEAAGACPEHHMSDDAKLGLILLFMVFA
                     LMVIGPVIAG"
     gene            complement(1792..>2653)
                     /locus_tag="VI195_00395"
     CDS             complement(1792..>2653)
                     /locus_tag="VI195_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEY6822869.1"
                     /translation="PDEAEAARLAAASCDVHSSVVCAGAWLDGYRLTDDYYQGDRAMR
                     ESGFDINFHFGPFGGSTHHYAAVGLNGLLYRYELDLADFARQLGRSTEAEHWARMAVA
                     RREAMDKYLWRPEAHRYADFDFVAQKPASTVYLTMFYPLWAGAASALQAASVRQQLPL
                     FERLGGLAMDDRASGAQWDEPFGWAPTNWLAVCGLAAYGFGDDARRVAEKFTTTIDRS
                     LAADGTIREKYNMVLGNADVQVTAGYTQNVVGFGWTNGVYLKMRELLAGNGRSGLCPM
                     VPTTTGAEAR"
BASE COUNT          463 a          913 c          848 g          429 t
ORIGIN      
        1 gccgccgcga tatcgagctc tccgagctcg gggcacggca agcgcacgcc acggcacggc
       61 gcatcgccgc ccagtgtgag ccgaccgcga tctactcgag cccgctgcgc cgctgtctgc
      121 agaccgccgc ggcgatcggc gccgcctgcg ggggtctcgc cacaacggtg ctcgaggatc
      181 tgaatgacct gcattacggt gactgggagt ggcacaccca cgaggaggtg cgcgcgcgct
      241 ggccggagct tctccagcgc tggttcgcga gcccgcagct cgtgcgcttc ccacggggtg
      301 aatcgctgca ggatctcgtt gcgcgcatgg cgaacgtcct gcggcgcgta cgcgagacgc
      361 atgcggatga gacggtcgtg gtggtcggcc acagcagcgg taaccgggca ctgctcctgc
      421 agacactcga tcagccgctc tcggcttact ggcggctggc gcaggatgcc tgctcggtga
      481 gcgacatcga gctgcaccca cacgccgcaa cgctgcggcg attcaacgag atctactggg
      541 gcagctcatc atgagccccg cgtccgtctc agctcctgca cccagtgacg caagtggtgc
      601 actcggccgc cggctcaccg cgttcgacat cgaggcacac cgcggcggcc gcgcgcgccg
      661 gccggaaaat acactccccg ctttcgcgga cgcgctgtcg atgggcgtcg ataccctcga
      721 gctcgacatg ggcgtcacgc gcgatggggt ggtggtggtc tcgcacgagc gtggcctcaa
      781 tcccgatctc acccggaccc ccgacggtga ctacattgcg gcggagattc ccttcgtcga
      841 cctgacgctc gcgcaggtga agcagttcga cgttggacag atccggcccg gcagtgcata
      901 tgcagcacgc ttcccggatc agcgcgcgat acccggcacg cgcatcccga cgctggccga
      961 ggtatttgcg ctggtccgtc gctcgggcga tcgccacgtg cgcctcaaca tcgagaccaa
     1021 gatcgacccg catcggcccg cggaggcact cgatccgcag cacttcgtaa ccgccgtcct
     1081 cgagctgctg cgccgtgaga gattcaatga ccgcgtcttg atccagtcct tcgactggcg
     1141 gacgctccag ctcatccagc agcaggctgc ggaggttccg acggtctatc tcacggagca
     1201 atcggggccc accgccaccg ttcatgccga tgcggcctct ctctggacgg ccggtttcga
     1261 tccgcgcgac cacggaggct cgctcccgcg caccatcaaa gccgccggtg gcagcatctg
     1321 gtcccccgac tacaacgatg tcgatgcacg ctcgatcgag gaagcgcacc ggcttggact
     1381 cccggtcatt gtctggaccg tgaaccaacc cgccgacatg gcgcggctga tcgatatcgg
     1441 ggtagacggc atcatctcgg accggccgga cctgctgcgg gcggaggccg cgaagaaagg
     1501 catcccgctc ccgccgcccg cgcccgtgtc cccgtagacc gtccataggt cacgggccag
     1561 caatcgagcg aaggggtgcg cgctcgagag agatgcgccg gctgagtcag cccgcgatga
     1621 ccgggccgat caccatgagg gcaaagacca tgaaaagcag aatcaagccc agtttggcgt
     1681 cgtccgacat gtggtgctcc gggcaggctc ctgccgcttc gatgcgctgc aaccatatgc
     1741 tggaccggta gcgcctgcgc gtgacgcgca tcacagtgtt tcaaatcgac atcagcgggc
     1801 ttcagcgccc gtagtggttg gcaccattgg gcagagtccg ctgcgcccat tgccggccag
     1861 aagctcccgc atcttcaggt atacgccgtt ggtccagccg aagccgacca cgttctgcgt
     1921 gtagccggcg gtcacctgaa catcggcgtt gccgagcacc atattgtatt tctcgcggat
     1981 cgtgccatcg gcggcgaggc tgcgatcgat ggtggtcgtg aacttctcgg caacgcgcct
     2041 ggcatcgtca ccgaatccgt aggcagcgag gccgcacacc gcgagccagt tggtcggtgc
     2101 ccagccgaag ggctcatccc attgcgcgcc actcgcgcgg tcgtccatgg cgaggccacc
     2161 caggcgctcg aaaagcggca gctgctgacg aacgctcgca gcctggagag ccgacgccgc
     2221 gccggcccac aagggataga acatcgtgag gtacaccgtg ctcgcgggct tttgcgcgac
     2281 gaaatcgaaa tccgcatagc ggtgagcctc cggccgccag aggtacttgt ccattgcctc
     2341 gcgccgtgcc accgccatgc gcgcccaatg ctcggcttcc gtactcctgc cgagctggcg
     2401 tgcgaaatcg gcgagatcga gctcataccg gtagagcaga ccgttcaagc ccaccgccgc
     2461 gtaatggtgc gtcgagccgc cgaagggacc gaagtggaag ttgatgtcga agcccgattc
     2521 gcgcatcgca cggtcgccct ggtagtagtc gtcggtcagc cgataaccat cgagccaggc
     2581 gccggcacag accaccgagg aatgcacatc gcaactcgcc gcagccaagc gcgcggcctc
     2641 ggcttcgtcc ggg
//
LOCUS       DATDMP010000022         2852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_892262, whole genome shotgun sequence.
ACCESSION   DATDMP010000022 DATDMP010000000
VERSION     DATDMP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892262"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VI195_00400"
     CDS             <1..362
                     /locus_tag="VI195_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY6822870.1"
                     /translation="VARYYELWSRDAPLRLPERGDEGHSWHVFTPLLPLAHLALSRAQ
                     FMEAMKAQGIGTGVHYPAIHLFSAYRALGYREGQFPNAERIGRETVTLPLFPGMQLDD
                     VDRVVRAVDAIVRGARR"
     gene            359..1399
                     /locus_tag="VI195_00405"
     CDS             359..1399
                     /locus_tag="VI195_00405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6822871.1"
                     /translation="MSAHARPDISVVIPVYNEEEGLPFLFERLYPALDALGRAYEVVF
                     VDDGSRDRSVAELREQFQRRPDVTRVVVLARNAGQHLAILAAFQQTRGRYVITLDADL
                     QNPPEEIARIVATMDAGADYVGTIRVSRQDVLWRKAASRLLNRVREGTTSIHITDQGC
                     MLRGYDRSVIDAVNRCTEVSTYVPALAYTFARHPVEIEVAHAERTVGQSKYSLFRLIR
                     LNFDLMTGFSVAPLQFFSMSGIVIALLSILFVIYLAIRRLIVGPEAEGLFTLFGIAFF
                     LIGVALIGLGVVGEYVGRIYEQVRQRPRYTVAAVLEQNTDTASAQLAHLPASATPAAQ
                     LAPVERVRGSVE"
     gene            1396..2322
                     /locus_tag="VI195_00410"
     CDS             1396..2322
                     /locus_tag="VI195_00410"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEY6822872.1"
                     /translation="MNRPRAVVFAYHDVGVRCLKALLSGGVAVPLVVTVPDDPAERRW
                     FASVADTAVDYGLDVVAPADASTAELTRRVTELQPDFLFSFYYRAMLGAPLLRAARRG
                     ALNMHGSLLPKYRGRAPVNWAILRGERESGATLHYMVERADAGDIVDSQAVPILLDDD
                     AREVFGKVTVAAETVLVRSLPGLIAGSAQRRPQPIVPGQYFGRRRPEDGRIDWSRTAL
                     EVHNLVRAVAPPFPGAFADVGGERWQIHRTRLTPQIVAPRGAWLFASADECYLACGDG
                     LALRLLAAEDPHGPVDLGKLALRAAQQPLPLA"
     gene            2327..>2852
                     /locus_tag="VI195_00415"
     CDS             2327..>2852
                     /locus_tag="VI195_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6822873.1"
                     /translation="MALIALKVDVDTYRGTREGALRLADLLERLDVRATFLFSLGPDH
                     TGRAIRRAFRPGFLGKVKRTSVLEHYGLKTLLYGVLLPGPRIGRKCRGEMQAIARRRF
                     EVGVHTWDHARWQDGVARASEEWTRRELTLAHAEFTEIFGRAPLVHGAAGWQMNRYVP
                     ALQQHLGFRYASDTR"
BASE COUNT          395 a          959 c         1002 g          496 t
ORIGIN      
        1 tggtcgcgcg ctactacgag ctgtggtcgc gggatgcgcc gctgcgcctg cccgagcgtg
       61 gcgatgaggg tcacagctgg cacgtgttta ctccgctgtt gccgcttgcg catctcgcgc
      121 tctcgcgcgc gcagttcatg gaggcgatga aggcgcaggg tatcggcacc ggggtgcact
      181 atccggcgat tcatctgttc agtgcctacc gggcgctcgg ctatcgcgag gggcaattcc
      241 cgaatgccga gcgcattggt cgcgagacgg tcacgctgcc gctgttcccg ggcatgcagc
      301 tggacgatgt cgatcgtgtg gtccgggcgg tcgatgccat cgtgcgcgga gcgcgacggt
      361 gagcgcgcac gcgcgtcccg atatctcggt cgtcatcccc gtgtacaacg aggaggaggg
      421 gttgccattc cttttcgagc ggctgtatcc ggcgctcgat gcgctcggcc gcgcgtacga
      481 ggtggtgttc gtcgatgatg gcagccgcga ccgctcggta gcggagctgc gcgagcagtt
      541 ccagaggcgt ccagacgtta cccgggtggt ggtgctggcg cgcaacgccg ggcagcacct
      601 ggcgatcctc gccgccttcc agcagacccg cggccgctac gtgatcaccc tcgatgccga
      661 cctgcagaat ccaccggagg agatcgcgcg catcgtcgcg accatggacg ccggcgccga
      721 ctacgtcggc accatccgcg tcagccgcca ggatgtgctc tggcgcaagg cggcgtcgcg
      781 cttgctcaac cgcgtgcgcg agggcaccac ctcgatccac atcaccgatc agggttgcat
      841 gttgcgcggc tatgaccgca gcgtcatcga tgcggtcaac cgctgcacgg aagtgagtac
      901 ctacgtgccg gcgctcgcct ataccttcgc gcgtcatccc gtggagatcg aggtggcgca
      961 cgcggagcgc accgtcggac agtcgaagta ctcgctcttc cggctcatcc ggctcaactt
     1021 cgatctgatg accggctttt cggtggcgcc cctgcagttc ttctcgatga gcggcatcgt
     1081 catcgcgctg ctgtcgatcc tgttcgtgat ctacctcgcc atccgccgcc tcatcgtcgg
     1141 ccccgaggcg gaggggctgt tcacgctgtt cggcatcgcc tttttcctca tcggcgtggc
     1201 gctgatcgga ctcggtgtcg tgggcgaata tgtcgggcgc atctacgagc aggtccggca
     1261 gcgtccgcgt tacacggtgg ccgcggtgct cgagcaaaac accgacacgg cgagcgcgca
     1321 gctcgcgcac ctgccggcat ccgcgacgcc cgccgcgcaa ctcgcaccgg ttgagcgtgt
     1381 gcgggggtcg gtggagtgaa ccgcccgcgg gcagtcgtct ttgcgtatca cgatgtcgga
     1441 gtccgctgcc tgaaggcact gctctcgggt ggggtggcgg tgccactcgt ggtgacggtg
     1501 ccggacgacc cggccgagag gcgctggttc gcgagcgtcg cggacacggc ggtggactac
     1561 ggtctcgatg tggtagcccc ggccgatgcc agcacggcgg agctgacccg ccgagtcacc
     1621 gagctgcagc cggacttcct gttctccttc tactaccgcg ccatgctcgg cgccccgctg
     1681 ctgcgtgcgg cacgccgcgg cgcgctcaac atgcacggat cgctgctgcc gaagtaccgg
     1741 ggccgcgcgc cggtgaactg ggccattttg cgcggtgagc gcgagtcggg tgccacgctg
     1801 cactacatgg tggagcgcgc cgacgccggc gacatcgtcg acagtcaggc agtcccgatc
     1861 ctgctcgacg acgatgcgcg ggaggtgttc ggcaaggtga ccgtcgcagc cgagaccgtg
     1921 ctggtgcgtt cgctcccggg cctgattgcc ggcagcgcgc agcgcaggcc gcaaccaatc
     1981 gtgcccggtc aatacttcgg ccgccggcgc ccggaggatg gccgcatcga ttggtcgcgg
     2041 acggcgctgg aggtccacaa tctcgtgcgg gcggtcgcgc cgcccttccc cggcgcgttc
     2101 gcggatgtcg gcggcgagcg ctggcagatt caccgcacgc ggctcactcc gcagatcgtc
     2161 gcgccgcgcg gtgcgtggct gttcgccagc gcggatgagt gttacctagc ctgcggcgat
     2221 ggcttggcgc tgcggctgct ggcggccgag gacccgcacg gacccgtcga tctcggcaag
     2281 ctggcgttgc gcgcggcaca gcaaccgttg ccgctcgcct agcgccgtgg cgctcatcgc
     2341 gctcaaggtc gacgtcgaca cctaccgtgg cacgcgcgag ggcgcgctgc gactcgccga
     2401 tctgctcgag cggttggatg tgcgggccac gtttctcttc agcctcggtc ccgatcacac
     2461 cggacgcgcg atccggcggg ccttccgccc cgggttcctc ggcaaggtga agcggacctc
     2521 cgtgctcgag cactacggcc tgaagactct tctctatgga gtgctgctgc ccgggccgcg
     2581 catcggccgc aagtgccgcg gcgagatgca ggcgatcgcg cggcgccggt tcgaggtcgg
     2641 cgtacacacc tgggatcacg cgcgctggca ggacggggtg gcgcgcgcga gtgaggagtg
     2701 gacgcggcgc gagctgacgc tggcgcacgc ggaatttacc gagatcttcg ggcgagcgcc
     2761 gctggtgcac ggcgcagccg ggtggcagat gaaccgctac gtgccggcac tccagcagca
     2821 tcttggcttc cgctacgcct ccgacacccg gg
//
LOCUS       DATDMP010000023         4212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_559336, whole genome shotgun sequence.
ACCESSION   DATDMP010000023 DATDMP010000000
VERSION     DATDMP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559336"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VI195_00420"
     CDS             complement(<1..444)
                     /locus_tag="VI195_00420"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6822874.1"
                     /translation="METTSQAPRAPVHRSGWQFWIDRGGTFTDVIGLSPAGGLHVRKV
                     PSISMDATGDPGVRAARTILLDAARTAGGAPAAAVAAVKVGTTVATNALLTRSGEPVV
                     LVTTAGFGEALRIGYQNRPDIFARNIVLPKRLYASVIEAQERIDAA"
     gene            498..1034
                     /locus_tag="VI195_00425"
     CDS             498..1034
                     /locus_tag="VI195_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6822875.1"
                     /translation="MQALVTSRHVVFSHGQEAGPWGRKITALAEVARTEGYDAHSVDY
                     RGIDEPRARVAKLVEFCKELTGDLVLVGSSLGGYVAVASASLLHARGVFLMAPALYMQ
                     GLPELRSGLLDCPAAVVHGWRDDVVPYEHSVRFAHGYRCALHLLESDHRLHNQVRVIQ
                     YLFEYFLIALDLPAIAFE"
     gene            1083..3512
                     /gene="plsB"
                     /locus_tag="VI195_00430"
     CDS             1083..3512
                     /gene="plsB"
                     /locus_tag="VI195_00430"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HEY6822876.1"
                     /translation="MNWLLRRLLALWVRYRVLPEDAAAQLRGRVRPLCYVLEQRSATD
                     LAVLQEACARLRIPRPGRPLAGGTHELRSYFWLAQTRGLLGRRIDRRPPRQLAHMIAA
                     LRSDPQADFELVPAAVYWGRAPQKEASWIGLLLSENFGVTSRARKFMQVLFNGRNTLL
                     ELDEPLSLRALLGEELAPAQRGFRVARALRALYTRRRAARIGPDLSHRRTIISQVLRT
                     RAVRAAVAQEMRERRLPRYKALQRARGYTWEIAANYSHAFVRFMEHALGWLWNRLYDG
                     VEFGHADTLAAVAEGNEIIYVPCHRSHMDYLLLGYAIYVHGYAVPHTAAGVNLNLPLV
                     GRLLRKGGAFFIRRSFAGNRLYTVVFMKYLAAIMSRGHAIEYFIEGGRSRTGRLLQPK
                     TGMLLMTVRSFLADPTRPVVFVPVYFGYERLVEGATYVGELSGRPKEKESIWGLLGSW
                     RVLRERFGKVYVNLGEPVALPAILDRHEPRWRERRFDDETRAAWLAGAVDELAERIMR
                     NINAAAAVTPINLLALALLAMPRQALPEADLMRQLELYRSLLTSFPYGNRVTVTRLSG
                     EEMVAYGLTLRLIEREPQHLGDIVRMTVENAVLATYFRNNVLHLFALPSLIACVFVSN
                     ARVSTADIQRLAGRVYPYIAAELFLEWREADLPTVVAAALAALAAHGLIESEGEGGGW
                     RRPPPASAQAMQLSLLAQATLQTIERYYMVVAQLVTAGSGALTQVALEERCQQTAQRM
                     TLLYGLDSPEFSDRTMFTNFIALLRSRGVIRAGAGGALQFDEVLVRVAQDAQFVLSEQ
                     LRHSILQIMHS"
     gene            3713..>4212
                     /locus_tag="VI195_00435"
     CDS             3713..>4212
                     /locus_tag="VI195_00435"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEY6822877.1"
                     /translation="MGFAATVACVMAALLPRAQAFSFRSDSGDWTGSWDTTIGYGMGW
                     RVSNPDCRLIAIANGGCGYSPNIDDGDLNYLHKAEYTEALTGVTELQLKYKERFGAFV
                     RASGLYDFSVMGNDVDRTPLSHEAKGVVGSYTRLLDAFGFLRFDLGSLPSELRAGRQV
                     VSWGEST"
BASE COUNT          649 a         1410 c         1398 g          755 t
ORIGIN      
        1 ggcggcgtcg atgcgctcct gcgcctcgat cacactcgca tacaggcgct tggggagcac
       61 gatgttgcgc gcgaagatgt ccggacgatt ctggtagccg atgcgcagcg cttcgccaaa
      121 cccggcggtg gtcaccagca ctaccggctc gccgctgcgc gtcagcagtg cgttggtggc
      181 taccgtggtg cctaccttca ccgccgcgac cgccgccgcg ggtgcaccgc cggcggtgcg
      241 cgccgcatcg aggaggatgg tgcgcgccgc acgcacgccc ggatcgcccg tggcatccat
      301 cgagatggag ggcaccttgc gcacgtgcag cccacctgcg ggcgacagcc cgatcacgtc
      361 ggtgaacgtt ccgccacgat cgatccagaa ctgccagccg ctgcggtgga cgggagcgcg
      421 cggtgcttgg cttgtggttt ccattgcctg cattattcgc ccatgctcct cgtgcgccca
      481 gcgccggatg ccctgccatg caagcgctcg tgacttcccg ccacgtcgtt ttctctcacg
      541 gccaggaagc cggaccctgg ggacgcaaga tcaccgcgct cgccgaggtg gcacgcaccg
      601 aaggatacga cgcacactcg gtggattacc gcggcatcga tgagccgcgg gcgcgcgtcg
      661 cgaagctggt ggagttctgc aaggagctca ccggcgatct ggtgctcgtt ggctcgagtc
      721 tcggcggcta tgtcgcggtc gcctcggctt cgctcctgca cgcccgcggt gtatttctga
      781 tggcgcccgc gctctacatg caggggctgc cggagctgcg cagcggactg ctcgattgtc
      841 cagccgcggt agtacacggc tggcgtgatg acgtggtgcc ctacgagcac agcgtacgct
      901 ttgcccacgg ttatcgctgc gcgctgcacc tgctcgagag cgatcaccgg ctgcacaacc
      961 aggtgagggt gatccagtac ctgttcgagt acttcctcat cgccctcgat ctgccggcga
     1021 tcgccttcga gtagcgatcg agccggctcg ccctcgcggc gcgtgcactc agcggacccg
     1081 gcatgaactg gctgttgcgc cgcctgttgg cactgtgggt gcgctaccgc gttctgccgg
     1141 aggatgccgc cgcgcagctg cgcggccggg tacggccgct gtgctacgtg ctcgagcagc
     1201 gcagcgccac cgatctcgcc gtgctgcagg aagcctgtgc ccggctccgt atccccaggc
     1261 cgggccggcc gctcgcgggc ggcacgcacg agctgcgctc ctatttctgg ctggcgcaga
     1321 cccgcggact gctcggccgg cgcatcgacc ggcggccgcc acgccagctc gcacacatga
     1381 tcgccgcgtt gcgctccgat ccgcaggcgg atttcgagct ggtgccggcc gccgtctact
     1441 gggggcgcgc accacagaag gaagcttcct ggatcgggct gctgctgagc gagaatttcg
     1501 gcgtcaccag ccgtgcccgc aaattcatgc aggtgctgtt caacggacgc aatacacttc
     1561 tcgagctgga tgagccgctg tcgctgcgcg cgctgctcgg cgaggagctc gctcccgcgc
     1621 aacgcggctt tcgcgtcgca cgcgccttgc gcgcgctcta cacccggcgg cgcgctgcgc
     1681 gcatcggacc ggacctctcc catcggcgca ccatcatcag ccaggtgctg cgcacgcgcg
     1741 cagtgcgtgc tgccgtcgcg caggagatgc gcgagcggcg cttgccgcgc tacaaggcgt
     1801 tgcagcgcgc gcgcggctac acctgggaga tcgccgccaa ctattcccat gccttcgtgc
     1861 gcttcatgga acatgcgctc ggctggttgt ggaaccggct gtatgacggt gtggagttcg
     1921 gccacgccga tactctggcc gcggtggcgg aagggaacga gatcatctac gtgccctgcc
     1981 accgcagcca catggactac ctgctgctcg gctacgccat ctacgtgcac ggctacgccg
     2041 tgccgcacac cgccgccggt gtcaatctca acctgccgct cgtcgggcgc ttgctgcgca
     2101 aggggggtgc cttcttcata cgccgcagct tcgccggcaa ccgcctttac acggtggtgt
     2161 tcatgaagta tctcgcggcc atcatgagtc gtggccacgc catcgagtac ttcatcgagg
     2221 gcgggcgaag ccggaccggg cggctgctgc agccgaagac cggaatgctg ctcatgacgg
     2281 tgcgcagctt ccttgccgat ccgacgcgtc cggtggtgtt cgtgccggta tatttcggct
     2341 acgagcgcct ggtcgagggt gccacctacg tcggggagct gtcgggtcgc ccgaaggaga
     2401 aggagagcat ctggggactg ctcggctcgt ggcgggtgct gcgcgagcgc ttcggcaagg
     2461 tgtacgtgaa cctcggtgag ccggtcgcgc tcccagcgat cctcgaccgg catgagccac
     2521 gttggcgcga gcgccgcttc gatgacgaga cgcgcgctgc gtggctcgcg ggggcggtcg
     2581 atgagctcgc cgagcgcatc atgcgcaaca tcaatgccgc cgcggcggtg accccgatca
     2641 acctgcttgc actcgcgctg ctggcgatgc cacgccaggc gctgccggaa gcggatctca
     2701 tgcggcagct cgagctgtac cgctcgttgc tcacatcctt cccgtacggc aaccgcgtga
     2761 cggttaccag gctttccggc gaggagatgg ttgcgtacgg gctgacgctg cggctcatcg
     2821 agcgcgagcc gcagcatctc ggagacatcg tgcgcatgac cgtcgagaat gcggtactcg
     2881 ccacctattt ccgcaacaac gtattgcacc tgttcgcgtt gccctcgctc atcgcgtgcg
     2941 tattcgtgag caacgcgcgc gtgtcgaccg ccgatatcca gcggctggcg gggcgcgtct
     3001 atccgtacat cgccgcggag ctgttcctcg agtggcgcga ggcggatctg cccacagtgg
     3061 tggcagcggc actcgccgcg ctcgcggcgc atgggctgat cgagtccgaa ggcgaaggcg
     3121 gcggctggcg gcggccaccg cccgcatccg cgcaggccat gcagctgtca ctgctcgcac
     3181 aggccaccct gcagaccatc gagcgctact acatggtggt cgcgcagctg gtgacggccg
     3241 ggagcggtgc gctcacccag gtggcgctcg aggagcgttg tcagcagacg gcgcagcgca
     3301 tgactctgct ctatggcctc gactcgccgg agttctccga ccgcaccatg ttcacgaact
     3361 tcatcgcact gctgcgaagt cgcggcgtga tccgcgctgg cgccggcgga gcactgcaat
     3421 tcgacgaggt gctggtacgt gtggcgcagg acgcacaatt cgtcctgagt gagcagctgc
     3481 gtcacagtat cctgcagatc atgcactcgt agaatgcttg ccgcgttgcg tcgccgagta
     3541 atagactgac cacacgcggg tttgtatttg cgcaacaaac aagaacgcga cgtggcttcg
     3601 ctgcacactg agctcgagtg acgcaaccag ccttcctggc agacacgagt cggcgcgagt
     3661 ctgcaaggga agcagggggg aacacatgag gagagttcac accgcccgtg ccttggggtt
     3721 tgcggcaacg gtcgcctgtg tcatggcggc gctgttgcca cgcgcacaag ccttcagctt
     3781 ccgcagcgac tccggtgact ggacgggcag ctgggatacc accatcggct acggaatggg
     3841 ttggcgtgtg tccaatcccg attgccggct gatcgcaatc gccaacggcg gctgtggcta
     3901 ctcccccaac atcgatgatg gcgatctcaa ctacctgcac aaggccgagt acaccgaggc
     3961 ccttaccggt gtcaccgagc tgcagctcaa atacaaggag cgcttcggcg ccttcgtgcg
     4021 cgcctcgggc ctgtacgact tctcggtcat gggcaatgac gtcgaccgta cgccgttgag
     4081 ccacgaggcc aagggtgtcg tcggcagcta tacccggctg ctggatgcct tcgggtttct
     4141 gcggttcgat ctcggcagcc tgccttccga gctgcgcgcg ggccgccagg tcgtctcctg
     4201 gggcgagagc ac
//
LOCUS       DATDMP010000024         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1448282, whole genome shotgun sequence.
ACCESSION   DATDMP010000024 DATDMP010000000
VERSION     DATDMP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448282"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VI195_00440"
     CDS             complement(<1..494)
                     /locus_tag="VI195_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822878.1"
                     /translation="MAAVFLTGCRQGPSAGEAPPAKARGKNAPELEGVTLKSEEIDKA
                     GIKTATLAGSSHTPESTGYAVVTTRETIAQVLADLTSAAAVERQSRSALTRARSLAGT
                     PGAMPIEAQETAERQAAVDHAAFELAERRLSATYGQNAPWKENYTSPLLASLARGDNK
                     LARVT"
     gene            complement(623..2035)
                     /locus_tag="VI195_00445"
     CDS             complement(623..2035)
                     /locus_tag="VI195_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6822879.1"
                     /translation="MLACGCATESPPQPPSIAPATFEHSAAAPRAPWPAQDWYRGFGS
                     GELDDFVDLAVRDNTDLTVARERVAQADARARQAGAAILPKVSVDGNATYLSGHSKQG
                     SGHELDWFAMLSASYEVDFWGKNRATANAARLQAGASRAERDTVALTLLGGVAGEYFQ
                     VLALRERLSIARANRDAAQKILAAVQARYDAGVASPTELASQKTALYAAQIVISDFEQ
                     LEMEARATLALLLGRPPENFEVRADNLDSLREPVVAAGLPSELLTRRPDVVMAETNLR
                     AAHANLAAARAAMFPNLTLTAAAGVQNPALPAIVNTIPGVGPSYSAIASLTQPVFDHG
                     RLAAQRDEALAKEQELLASYRAAIIASLADVEKALSAVQHLEAVRDFQKGSVAESERA
                     FEGAQLRYQRGSGDFLTLLQSQRTLYAARDQFAQYRLARLEALVALCKALGGGWNASA
                     ATTRASEVAPAARAHERASS"
     gene            complement(2221..>2679)
                     /locus_tag="VI195_00450"
     CDS             complement(2221..>2679)
                     /locus_tag="VI195_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822880.1"
                     /translation="KNQMLAFDARTLKVLHRWPLTSCEGPSGLSMDREHRRLFSACDN
                     QTMVVMDADSGKVVAALPTGAGTDASVFDPSSQNVFASAGGCGTLTVIHEDAPDHFRV
                     LDNVSTASGARTMALDEKTHDVLLVTARHGHGANYRQLLPDTFVVLVVGK"
BASE COUNT          446 a          890 c          881 g          462 t
ORIGIN      
        1 gtgacgcgcg ccagtttgtt atcgccgcgg gcgagcgatg ctagaagcgg actcgtgtag
       61 ttttctttcc agggggcgtt ctgaccatag gttgccgaca aacgtcgctc ggcgagctcg
      121 aacgccgcgt ggtcgaccgc ggcctggcgc tcggccgtct cctgcgcttc gatgggcatc
      181 gcccccgggg ttccggcgag actgcgggcg cgagtgagcg cggagcgact ctgccgctcg
      241 actgcggctg cactcgtgag gtccgcgagc acctgtgcaa tcgtctcgcg cgtggtcacg
      301 accgcgtagc ctgtgctttc gggcgtgtga ctggacccag cgagcgtggc ggtctttatg
      361 ccggccttgt cgatctcctc ggacttcagc gtgacacctt caagctcggg cgcgtttttg
      421 cccctcgcct tcgccggcgg ggcttcgccc gctgagggcc cctgccggca gcccgtaagg
      481 aaaacggcag ccatgcacgc cgcgacgatg aatgcgaccg ccgccccctg gcatgagcag
      541 attcgcgcgg gcggcgcgtg cgggcaacgc cggacgcgga tcatcgcagg gtcttcatgc
      601 aggtaaccgg tggctgctga ctttaggagg aggcacgctc gtgagcccga gcggcaggcg
      661 cgacctctga agcgcgcgtt gtggcggcgg acgcattcca gccgccaccc agcgccttgc
      721 acagcgccac gagcgcctca aggcgcgcca ggcggtactg tgcaaactgg tcacgggcgg
      781 catacagggt gcgctgcgat tgcagcagcg tgagaaagtc gccggatccc cgctgatagc
      841 gcagctgcgc tccctcaaag gcgcgttcgc tctccgccac gctgcccttc tggaagtcgc
      901 gcaccgcctc gaggtgttgc acggcggaga gcgctttctc gacatccgcc agggaggcga
      961 tgatcgccgc gcgatagctc gcgaggagtt cctgctcctt ggccagcgct tcgtctcgct
     1021 gcgccgccaa acggccgtga tcgaagaccg gctgcgtgag gctcgcgatc gccgaatacg
     1081 agggaccgac gcccgggatg gtattcacga tggccggcag cgctggattc tgcacaccgg
     1141 cggcggcggt gagcgtcaga ttggggaaca tcgccgcgcg cgctgccgcg agattcgcgt
     1201 gcgcggcgcg cagattcgtt tctgccatta ccacatcggg ccggcgggta aggagctcag
     1261 acggcagtcc tgccgcgacc accggctcgc gcaatgaatc gagattgtcc gcccgaacct
     1321 cgaagttctc cggaggacgt cccagaagca gcgcgagcgt ggcgcgcgcc tccatctcca
     1381 gctgctcgaa gtccgatatg acgatctgcg ccgcgtacag ggcggtcttc tgcgaggcga
     1441 gctcggtggg acttgcgacc cccgcgtcgt agcgcgcctg aacggcagcg agaatcttct
     1501 gtgccgcatc ccggttggct cgagcgatcg agagacgctc acgcagcgcc agcacctgga
     1561 aatactcgcc ggcgacgccg ccgagaagag tgagtgcgac cgtgtcgcgc tcggcgcgcg
     1621 aggcgccggc ctgaagtcgt gccgcgttcg cagtcgcgcg attctttccc cagaagtcaa
     1681 cttcatagct ggccgagagc atcgcgaacc agtcgagctc gtgaccgctt ccctgcttcg
     1741 aatgacccga gaggtaggtc gcgttgccat cgacgctcac cttcggcagg atcgcggctc
     1801 ccgcctggcg cgcacgtgcg tcagcctgag cgacgcgctc gcgcgcaacc gtcagatccg
     1861 tgttgtcgcg gaccgcgagg tcgacgaagt cgtcgagctc accgctgccg aaccctcgat
     1921 accagtcctg agcgggccat ggcgcgcgcg gagcagcggc actatgctcg aatgtagcgg
     1981 gtgcgatgga cggcggttgc ggcgggctct cggtcgcgca accgcacgcg agcaagacaa
     2041 accataacgc cgaggccagc cagcgatcgc aggccccccg cgcatccgca caatccgcgc
     2101 gcatgcgtgc atgctacccg agcaaccttt ggtttcgctt aaaggcagcg tctgccgagc
     2161 ttcgctgccg caccatgaaa ccgttgtgcg tcgaggactc gccccggttc gctcgcctca
     2221 tcacttaccg actacgagga cgacgaatgt gtccggaagc agctgccgat aattcgcacc
     2281 atgaccgtgg cgagccgtga ccagcagcac atcgtgcgtt ttctcatcca gcgccattgt
     2341 gcgtgcaccc gaggctgtcg agacgttgtc gagcacacgg aaatgatcgg gtgcatcctc
     2401 gtggatgacc gtcagggttc cgcatccgcc cgcggaggca aacacgttct gcgacgacgg
     2461 atcgaacaca ctggcgtccg tgcccgcgcc ggttggtagc gcagccacga ctttgccgct
     2521 gtcggcatcc atgacgacca tcgtctggtt atcgcaggcg gaaaagagcc gtctatgctc
     2581 gcgatccatc gaaagccccg atggcccttc gcatgaggtg agcggccaac gatgcaggac
     2641 cttcaacgtt ctggcatcga aggccagcat ctggttctt
//
LOCUS       DATDMP010000025         4585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_559548, whole genome shotgun sequence.
ACCESSION   DATDMP010000025 DATDMP010000000
VERSION     DATDMP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559548"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VI195_00455"
     CDS             <1..545
                     /locus_tag="VI195_00455"
                     /inference="COORDINATES: protein motif:HMM:NF026109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6822881.1"
                     /translation="SGAEYPADLVIAGVGVAPADELASAAGLECANGVVTDVYCRTAD
                     EAIYAAGDCASHLNRQYGRHLRLESVDNAFEQGTTVALNLLGTQTLHDKVPWFWSDQF
                     DLKLIIIGVGYGYDTAIVRGSPAARAFSICYLRNGELVALDSVNTPKDQMAARKLIAA
                     HARPDVAKLADSGVSLRDTV"
     gene            complement(571..4053)
                     /locus_tag="VI195_00460"
     CDS             complement(571..4053)
                     /locus_tag="VI195_00460"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEY6822882.1"
                     /translation="MSQRIRSVLVANRGEIAIRVMRAASELGLRTVAVYSHEDRFSLH
                     RTKADEAYLVGRGKGAVEAYLEIEELLRVAREAAVDAIHPGYGFLAENPQFARACRAA
                     GMCFIGPEAETLAVFGNKLRARELAVSAGVPVMPASGPLPADPAACAQAAERIGYPLM
                     LKASWGGGGRGTRVVRDAAQLAELLPVCRREAQTAFGSDEVYLEKLVSRARHVEVQIL
                     GDLDGTLVHLFERDCTAQRRHQKVVERAPAVFLSEAQRGTLCDAALAIGRAARYTSVG
                     TVEFLQDADDGRHYFIEVNPRIQVEHTVTECVTGIDLVKAQIRIAGGARIGTPESGVP
                     AQPDIRLSGHALQCRITTEDPENNFVPDYGKIAAYRSPAGFGIRLDAGTAYTGAIITR
                     SYDSLLVKVTAWAPTAEESIARMHRALAEFRIRGVVTNLRFLDQLITHARFAAGDYTT
                     RFIEDTPELLRWPRKRDRATRILQLLGATIVNGNAEVLGRARPARLLSPQLPPGPLPA
                     PVPGSKQRFDRLGAEAFAQWMLDEPRVLITDTTMRDAHQSLLATRVRSFDMTAIAPYY
                     AALLPQLFSLECWGGATFDVALRFLREDPWERLSALRERLPNLLLQMLLRGANAVGYT
                     NYPDNVVRFFIARAAARGIDVFRIFDSLNWIENMRVSIDAVREAGKLCEAAICYTGNL
                     SDPGERKYTLDYYLKLARELRAAGTHVLGIKDMAGLLRPRAAYELVKALKEETGLPVH
                     LHTHDMSGIAAASVLAAVAAGADAIDAAIDAMSGLTSQPNLGSLVEALRFGPRDPGLD
                     GDAVRRISAYWEQVRRQYTAFESDIRAGASEVYVHGMPGGQYTNLREQARALGIEEAR
                     WPEVARTYAEVNELFGDIIKVTPTSKVVGDLALLMASTGITRAQVLDPDTAVAFPESV
                     VQLLRGELGQPHGGFPQALQAKVLQGAAPLATRAGAALAPVDLDAERARLQSRSPLPI
                     TEEDLASYLMYPRVFEEYARNRALYGNPAILPTPVFFYGMEPGQEITVDLERGKTLII
                     RYLAVSEPHADATRTVFFEMNGQPRSVRVADQRQVATRPPPAKAVAGNPQHVGAPMPG
                     VVSTVAALAGAQVSRGDTLLTLEAMKMETTVRAEVAGEVAEVLVRPGQSVEAKDLLVV
                     LRAEAHVPSKESSR"
     gene            4151..>4585
                     /locus_tag="VI195_00465"
     CDS             4151..>4585
                     /locus_tag="VI195_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY6822883.1"
                     /translation="MSRRTLTGITAALLAVLLPGVAAAAASSAPVAIVIHGGAGVIER
                     SKMTAEREASYRLGLAAALDAGYEVLERGGTSLDAVIAAVERMEDDPQFNAGRGAVLD
                     EDGDAELDAAIMDGAGPRAGAVAAVRHVKNPIDLARLVMDKSP"
BASE COUNT          721 a         1585 c         1562 g          717 t
ORIGIN      
        1 agagcggcgc cgagtatccg gccgacctgg tgatcgctgg cgtcggagtc gctcccgcgg
       61 atgagctcgc gagcgccgcc gggctcgagt gcgcgaacgg cgtcgtgacg gatgtgtatt
      121 gccgcactgc ggatgaggcg atctatgccg ccggtgactg cgcgagccat ctgaaccgcc
      181 agtacggccg ccacctgcgg ctcgagtccg tcgacaacgc cttcgagcag ggcaccacgg
      241 tggcgctgaa cctgctcggc acgcagacgc tgcacgataa ggtgccatgg ttctggtcgg
      301 accagttcga tctcaagctg atcatcatcg gggtcggata cggctacgac accgccatcg
      361 tgcgcggctc gcccgccgca cgcgccttca gtatctgcta tctgcgcaac ggcgagctcg
      421 tggctctcga cagcgtcaac actcccaagg accagatggc ggcgcgcaag ctgatcgcgg
      481 cgcatgcccg tcccgatgta gccaagctcg ccgactccgg cgtgtcgctg agggataccg
      541 tctgagtgct cgatggtgct cccggtaacc tcacctcgag ctctccttcg acggaacgtg
      601 cgcctcagcg cgcagcacca ccaacagatc cttcgcctcc accgactgtc cagggcgcac
      661 cagcacctcc gcaacctcgc ccgcgacctc ggcgcgcacg gtggtctcca tcttcatcgc
      721 ctcgagggtc agcaaggtat cgccgcgtga cacctgcgcc ccggccagcg ccgcgaccgt
      781 cgacacaacc cccggcatcg gcgcacccac gtgttgcgga tttccggcta cggccttggc
      841 tggcggcggc cgcgtggcaa cctggcgctg atcggctacg cgcaccgagc ggggctggcc
      901 gttcatctcg aagaacacgg tgcgggtcgc gtccgcgtgc ggctcgctca ccgccaggta
      961 gcggatgatg agcgtcttgc cgcgctcgag gtcgaccgtg atctcctgtc ccggctccat
     1021 gccgtagaag aacaccggcg tcggaaggat cgcgggattg ccatagagcg cgcggttgcg
     1081 ggcgtattcc tcgaacactc gcggatacat caggtaggaa gccagatcct cctcggtaat
     1141 gggaagcggc gaccggctct gcagccgcgc gcgctcggca tcgaggtcga cgggcgcgag
     1201 cgctgcaccg gcgcgcgtcg cgagcggcgc cgctccctgc agcaccttgg cctgcagagc
     1261 ttgcggaaag ccgccgtggg gttgaccgag ctcaccacgc agcagctgca ccaccgactc
     1321 ggggaaggcc accgcagtgt ccggatcgag cacctgtgcg cgcgtgatgc ccgtgctcgc
     1381 catgagcagc gccaggtcgc cgacgacctt ggaggtcggt gtgaccttga tgatgtcgcc
     1441 gaagagctcg ttgacctccg cgtaggtacg cgccacctcc ggccagcgcg cttcctcgat
     1501 gccgagcgcg cgcgcctgct cgcgcaggtt ggtgtactga ccgcccggca tgccgtgcac
     1561 gtacacctcg gaggcaccgg cgcggatatc gctctcgaag gcggtgtatt gccggcgcac
     1621 ctgctcccag taggcggaga tgcgccgcac cgcgtcgcca tccaggcccg ggtcgcgcgg
     1681 accgaaacgc agcgcctcga cgagtgagcc gaggttcggc tgcgaggtga ggccgctcat
     1741 tgcatcgatg gccgcatcga tcgcatcggc gcccgcggcc accgcggcga gcacactcgc
     1801 cgcggcgatg ccactcatat cgtgcgtgtg cagatgcacc gggagtccgg tctcctcctt
     1861 gagtgccttc accagctcat acgcggcgcg cggccgcagc aggcccgcca tgtccttgat
     1921 gccgagcacg tgggtgccgg ccgccctgag ctcgcgcgcg agcttgaggt aataatccag
     1981 ggtgtatttg cgctcgcccg ggtcgctcag gttgccggtg tagcagatgg ccgcctcaca
     2041 cagcttgccg gcctcgcgca ccgcgtcgat cgaaacccgc atgttctcga tccagttgag
     2101 cgagtcgaaa atgcggaaga catcgatgcc gcgcgcggcg gcacgcgcga tgaaaaagcg
     2161 cacgacgttg tccggatagt tcgtatagcc gaccgcattg gcgccgcgca gcagcatctg
     2221 cagcagcagg ttcggcaggc gctcgcgcaa cgcgctgagg cgctcccacg gatcctcgcg
     2281 caggaagcgc agcgccacat cgaaggtggc gccgccccag cactcgagcg agaacagctg
     2341 cggcagcagc gccgcgtaat aaggcgcgat ggccgtcatg tcgaagctgc gcacccgggt
     2401 ggcaagcagc gactggtgcg catcgcgcat ggtggtgtcg gtgatgagca cgcgcggctc
     2461 atcgagcatc cactgcgcga acgcctcggc gccgagccga tcgaacctct gcttgctgcc
     2521 gggcaccggt gccggcagcg gccccggcgg cagctgcggg ctgaggagcc gcgccgggcg
     2581 ggcgcggccg agcacctcgg cgttgccgtt cacgatggtg gcaccgagca gctgcaggat
     2641 gcgcgtggcg cgatcgcgct tgcgcggcca gcgcagcagc tccggcgtgt cctcgatgaa
     2701 gcgcgtggtg taatcgccgg cggcgaagcg cgcatgggtg atgagctggt cgaggaagcg
     2761 caggttggtg actactccac ggatgcggaa ctccgcgagc gcacggtgca tgcgcgcgat
     2821 gctctcctcg gcggtcggcg cccaggcggt gactttgacc agcaacgagt cgtacgagcg
     2881 ggtgatgatg gcgccggtat acgccgtacc ggcatcgagc cggatgccga accccgccgg
     2941 actgcgatat gccgcgatct tgccgtagtc cggaacgaag ttgttctccg gatcctcggt
     3001 agtgatgcgg cattgaagcg cgtggccgct caagcggatg tcgggctgcg ccggcacgcc
     3061 gctctcgggg gtaccgatgc gcgcgccgcc ggcaatgcgg atctgcgcct tcaccaggtc
     3121 gatcccggtg acgcactcgg tgacggtgtg ctcgacctgg atgcgcgggt tcacctcgat
     3181 gaaataatgc cggccgtcat cggcatcctg caggaactcg accgtgccca cgctggtgta
     3241 gcgcgccgcg cggccgatgg cgagcgcggc atcgcacagc gtgccgcgct gcgcctcgct
     3301 caggaacacg gccggagcac gctcgaccac tttctggtga cggcgctgcg cggtgcagtc
     3361 gcgctcgaac agatgcacga gcgtgccgtc caggtcgccg agaatctgca cctccacgtg
     3421 ccgtgcccgt gacacgagct tctcgagata gacctcgtcg ctgccgaagg cggtctgcgc
     3481 ttcgcgccgg cataccggca gcagctcggc gagttgcgcc gcgtcccgca ccacgcgcgt
     3541 gccgcgaccg ccacctcccc agctcgcctt cagcatcagc gggtagccga tgcgctcggc
     3601 cgcctgcgcg cacgccgccg gatccgccgg cagcggcccg ctcgcgggca tcaccggcac
     3661 gccggccgaa accgcgagct cgcgggctcg cagcttgttg ccgaacaccg cgagtgtctc
     3721 ggcctccggc ccgatgaagc acatccccgc cgcacggcag gcgcgtgcga actgcgggtt
     3781 ctcggcgaga aatccatagc ccgggtggat cgcgtcgacg gccgcctcgc gcgcgacgcg
     3841 cagcagctcc tcgatctcga ggtaggcctc gaccgctccc ttgccgcggc cgacgagata
     3901 ggcttcgtcc gccttggtgc ggtggagcga gaaacggtcc tcgtgcgagt acaccgcgac
     3961 cgtgcgcagc ccgagctcgc tcgccgcgcg catcacacgg atggcgatct cgccgcgatt
     4021 ggcgaccagc acactgcgta tccgctgcga catcgcgggt ttcaccttaa gatctaccgt
     4081 ccggattgaa ggcgctcacc gctcgcgcgc gtagtggctg agcgcttcga tcatagcctg
     4141 aaaggaaacg atgtcgcgcc gcacgcttac cggaatcact gctgcgctgc tcgccgtgct
     4201 gctgcctggc gttgcggccg cggcggcgtc gagcgcaccg gtcgccatcg tcatccacgg
     4261 gggtgcggga gtcatcgaac gctccaagat gacagccgag cgcgaagcct cctaccgctt
     4321 gggtcttgcg gcggcgctcg atgccggcta cgaggtgctc gaacgcggtg gcacgagcct
     4381 cgacgccgtg atagcggccg tcgagcgcat ggaggatgac ccgcaattca atgccgggcg
     4441 cggcgcggtg ctcgatgagg acggcgatgc cgagctcgat gcggccatca tggatggcgc
     4501 cggtccgcgc gccggcgcgg tggccgcggt gcgccacgtg aaaaatccca tcgatcttgc
     4561 gcggctggtg atggacaaga gcccg
//
LOCUS       DATDMP010000026         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1670620, whole genome shotgun sequence.
ACCESSION   DATDMP010000026 DATDMP010000000
VERSION     DATDMP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670620"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VI195_00470"
     CDS             <1..234
                     /locus_tag="VI195_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6822884.1"
                     /translation="DDLVRIRFRDSGPGVSNPETLFKPFHPGASSTGLGLYISRAVLK
                     SYGGDLHVEPEAQGGCFVVQLWAAEDGGSSGRA"
     gene            236..868
                     /locus_tag="VI195_00475"
     CDS             236..868
                     /locus_tag="VI195_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6822885.1"
                     /translation="MAAVNVLLIDDHLSFGQSVARALSAEPDLAVSSCTSISAALEIL
                     QRQHIDVVLLDHDLGVERASQFLPAARERGFSGRVLIVTAWVSDTEAKRLLRQGVAGI
                     FSKESPLEALVESIRLIQGGAICLDRRYRSLTDAEPTEQPGTGPRFNERQRKVLRFVL
                     EGLSNKVIAAQLHISESYVKALIQSLFKKTGVRTRGQLVRVTLEHYQDQL"
     gene            complement(975..1361)
                     /locus_tag="VI195_00480"
     CDS             complement(975..1361)
                     /locus_tag="VI195_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822886.1"
                     /translation="MNIRTLQRGLKYRGRVAAVRQTYHVFEAEGYFFVLSFALSKARR
                     GSGYFNVIDTDAVEYVQQRVGGTRGVTAKDVVAAARRTRYVPTTLAALNVLYILVALQ
                     SASIVRAGKNRQLFFSVKKGSAVRRS"
     gene            1669..>2597
                     /locus_tag="VI195_00485"
     CDS             1669..>2597
                     /locus_tag="VI195_00485"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6822887.1"
                     /translation="MMRAFVFSTLSVTLAMLCATHIACAQTAPASGDPWCRSKPEPAF
                     CHAVRGVRASGWPAQTRSEVMAPNGMVVTSQPLAAQAGLQVLMRGGNAVDAAVATAAV
                     LSITEPMMVGIASDLFAVIYIAKDHKAHVLNASGTAPSGATLAHLNELGYHWDPKNWG
                     PSSGMPTAGILPVTVPGTVWGWQAALQRFGRLSFREVLETAARYAQQGFPVSERIASD
                     WTLPNALPLRGCCTQLDPDSVKVWYMNGQPPAPGQLFSNPDLARTLRLLQEQGADAFY
                     KGEIARAIVAKSQALGGTMTLADLAEYHGEWVEP"
BASE COUNT          464 a          826 c          843 g          464 t
ORIGIN      
        1 gatgacctgg tgcgcatccg cttccgcgac tccggtcccg gagtgtcgaa tcccgagact
       61 ttgttcaagc cgtttcaccc gggcgcgtct tcgaccggcc tggggctgta catttcgcgc
      121 gcggtattga aatcctacgg cggcgatctg cacgtcgagc ctgaggcgca aggtggctgc
      181 ttcgtagtac agctctgggc ggcggaagat ggaggcagct caggacgagc gtagcgtggc
      241 tgccgtcaac gtcctgttga tcgatgacca tctgtctttc ggacagagcg tggcacgtgc
      301 gttgagcgca gagcccgatc tcgcggtgtc gagctgcacc tcgatcagcg ctgctctgga
      361 gatcctgcaa cggcagcaca tcgatgtcgt tctgctcgac catgatctcg gtgtcgaacg
      421 ggcctcgcag ttcctgccgg ccgcccggga gcgcggcttc agcgggcgcg tgctgatcgt
      481 gaccgcttgg gtgagcgata ccgaggccaa gcgtctgtta cggcagggag tcgccggcat
      541 tttttccaag gagagtccac tcgaggctct ggtcgagagc atccggctga ttcaaggagg
      601 agcgatctgt ctcgacagac gctaccgcag cctgaccgac gccgagccca ccgagcagcc
      661 aggcaccggg ccgcgcttca atgaacggca acgcaaggta ttgcgcttcg tgctcgaggg
      721 gctgtcgaac aaggtgatcg cagcacagct gcatatctcc gagagttacg tcaaagctct
      781 gatacagagc ctgttcaaaa agaccggcgt gcgcacgcgc ggccagctgg tacgggtgac
      841 gctggagcac taccaggacc agctgtagcg atgcgcgcag cgctgaggcg ccagcctcgc
      901 cccgccacag cggcgaagct cgcagccgca gtcaccgccg ccgctgcgcg tgcgcgatca
      961 ttgcggcctg cggctcacga ccgtcgaact gcggagcctt tcttcacgga gaaaaacagc
     1021 tgccggttct tgcctgcgcg tacgatgctc gccgattgca gcgcgaccag gatgtaaagc
     1081 acattgaggg cggcgagcgt ggtaggcacg taacgtgtgc ggcgcgctgc ggccacgacg
     1141 tcttttgcgg tcactccgcg agttccgccc acacgctgct gcacatactc gacagcatcg
     1201 gtatcgatca cattgaagta gcccgagccg cgccgcgcct tcgagagcgc aaacgacagc
     1261 acgaagaagt aaccctcggc ctcgaacacg tggtaggtct gacgaaccgc cgcaacgcgg
     1321 ccacgatatt tgagtccacg ctgcagcgta cggatattca ccagtacccc cctcttcccg
     1381 gaaacagagc gcctgtcctc tgcggcgcac tctactccat acccgcaagc ttcgcgggct
     1441 gggctcaacg ctatcgcggt cattgccggg aagctccaac agctgtccgg cggctgtcgc
     1501 atgagctacc gatgttccat ggaaatcaac gagctacggg tgttggctcg ccgcggtcgc
     1561 agaaactgac agcacttcgc aatgtgcggt tctgtacagg cggcgaactc gctagtctgt
     1621 cgcatcgggg tggccaaccg tccggatcgc tcggtcacgg gggaatcgat gatgcgcgcg
     1681 ttcgtgtttt ccaccttgag tgtgacactc gcaatgttgt gcgcgacgca cattgcctgt
     1741 gcacagaccg cgcccgcctc cggcgatccg tggtgcaggt ccaagcccga gccggcgttc
     1801 tgtcatgcgg tgcgcggcgt gcgcgcctcg ggttggccgg cgcagacgcg ctcggaggtc
     1861 atggctccga acggcatggt ggtcacgagc cagcctttgg cggcgcaagc gggcctgcag
     1921 gtgctgatgc gcggtggcaa tgcagtggat gccgccgttg cgaccgcagc ggtgctcagc
     1981 atcaccgagc ccatgatggt cggtatagcg tccgacctct tcgcagtcat ttatatcgcc
     2041 aaggaccaca aggcgcacgt gctgaacgcc agcggtaccg cgccgagcgg cgcgacgctc
     2101 gcgcacttga acgagctcgg ctatcactgg gacccgaaga actggggtcc gagctcgggg
     2161 atgccgacgg cgggaattct gcccgtgacg gtgcccggta cggtgtgggg ctggcaggcg
     2221 gcgctgcagc gattcggcag gctctccttc agggaagttc tcgagacggc tgcgcgctac
     2281 gcgcagcagg gtttccccgt ctcggaacgc atcgccagcg actggaccct gccgaatgcc
     2341 ctgccgctgc ggggctgttg cacgcagctc gatccggact cggtcaaggt ctggtacatg
     2401 aacggacagc cgccggctcc cggccagctc ttcagcaacc ccgatctggc gcgcaccttg
     2461 cgtctgctgc aggagcaagg tgcagacgcc ttctataaag gagagatcgc ccgagcgatc
     2521 gtcgcaaagt cgcaggcact cggcggcacc atgacgctgg cagacctcgc cgagtaccac
     2581 ggcgaatggg tcgagcc
//
LOCUS       DATDMP010000027         3832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_670862, whole genome shotgun sequence.
ACCESSION   DATDMP010000027 DATDMP010000000
VERSION     DATDMP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670862"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2469
                     /gene="rpoB"
                     /locus_tag="VI195_00490"
     CDS             <1..2469
                     /gene="rpoB"
                     /locus_tag="VI195_00490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY6822888.1"
                     /translation="ALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSL
                     SVYARTNGYGFLETPYRRVENGHVTDKIDYLSAIEEGEFAIAQANAVLGPDGALTDQL
                     VSCRFQNEFTLMPRERINYMDVSPKQIVSVAASLIPFLEHDDANRALMGSNMQRQAVP
                     TLRSETPLVGTGMERPVAIDSGVTVIAKRGGTVDSVDASRIVVRVNDEETTAGEPGVD
                     IYNLTKYTRSNQNTCINQRPLVNAGDVIARGDVLADGPSTHLGELALGQNLLVAFMPW
                     NGYNFEDSILISERVVQEDRFTTIHIEELTCVARDTKLGPEEITADIPNVGDAALAKL
                     DEAGIAFIGAEVKAGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTGLRVPPG
                     MDGTVIDVRVFTRDGVDKDSRALAIEKSEIERVRTDFADQQRILEDDLFLRVRGMLTG
                     QSAAGGPHKLKAGTPIASEYLDEVPREEWFEIRLEDEEANSQLEQSAERLKLQRKAFE
                     KRLDDKKAKITAGDDLAPGVLKMVKVYLAVKRRVQPGDKMAGRHGNKGVISTIVPVED
                     MPYLEDGTPVDVVLNPLGVPSRMNVGQVLETHLGWAAKGVGLKIGRMLEQQAAAADLR
                     AFVRQIYNETGGQREEVDSLDDADLTQLARNLASGVPMATPVFDGASEEEIKRMLKLA
                     DLPLNGQTYLYDGRTGERFERQVTVGYMYMLKLNHLVDDKMHARSTGPYSLVTQQPLG
                     GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMDAG
                     MPESFNVLVKEIRSLGINMELEQD"
     gene            2599..>3832
                     /locus_tag="VI195_00495"
     CDS             2599..>3832
                     /locus_tag="VI195_00495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666357.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY6822889.1"
                     /translation="MKDLLKLFKQHAPVEHFDSIKIGLASPEMIRAWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVVCEKCGVEVTQSKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLMLDMTLREIERVLYFEAFVVIDPGMTPMQRGQLL
                     TDEMYLEAIEEHGDEFDARMGAEAVYELMRTIDLSAEIAKVREEMAGTSSETKLKRLS
                     KRLKLIESFVESGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLRRLLELNAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPQLRLHQCGLPKKMALELFKPFIFQKLQLRGEAS
                     TIKAAKRLVEREGPEVWDI"
BASE COUNT          738 a         1236 c         1263 g          595 t
ORIGIN      
        1 gcgctcggcc ccggcgggct cacccgcgag cgcgcaggct tcgaggtgcg cgacgtgcac
       61 ccgacgcact acggccgcgt atgcccgatc gagaccccgg agggcccgaa catcggcctg
      121 atcaattcgc tctcggtcta cgcgcgcacc aacggttacg gcttcctcga gacgccctac
      181 cggcgtgtcg agaacggcca cgtcaccgac aagatcgact acctctccgc catcgaggag
      241 ggtgagttcg ccatcgccca ggcgaatgcg gtactcgggc ccgacggcgc gctcaccgat
      301 cagttggtgt cctgccgttt ccagaacgag ttcacgctca tgccgcgcga gcgcatcaac
      361 tacatggatg tgtcgccgaa gcagatcgtg tcggtggccg cctcgctcat cccgttcctc
      421 gagcacgacg atgccaatcg tgcgctcatg ggctcgaaca tgcagcgtca ggcggtgccg
      481 acgctgcgct cggagacccc gctggtcggt accggcatgg agcgtccggt ggcgatcgac
      541 tcgggcgtca ccgtcatcgc caagcgcggc ggtaccgtgg actcggtcga cgcttcgcgc
      601 atcgtggtgc gcgtaaacga cgaggagacc accgccggcg agcccggcgt ggacatctac
      661 aacctcacca agtacacgcg ctccaatcag aacacctgca tcaaccagcg cccgctggtg
      721 aatgccggcg atgtgatcgc gcgtggcgac gtgctggcgg atgggccgtc gacgcatctc
      781 ggcgagctcg cgctcgggca gaacctgctg gtcgcgttca tgccgtggaa cggctacaac
      841 ttcgaggatt cgattctcat ctccgagcgc gtggtgcagg aggatcgctt caccaccatc
      901 cacatcgaag agctcacctg cgtggcgcgc gacaccaagc tcggaccgga ggagatcacc
      961 gccgacatcc ccaacgtcgg cgatgcggcg ctcgccaagc tcgatgaggc gggcatcgcc
     1021 ttcatcggcg cggaagtgaa ggcgggcgac atcctggtcg gcaaggtaac cccgaagggc
     1081 gagacgcagc tcactcccga ggagaagctg ctgcgcgcga tcttcggcga gaaggcatcc
     1141 gatgtgaagg acacgggcct gcgcgtgccg cccggcatgg atggcaccgt gatcgacgtg
     1201 cgcgtgttca cccgcgacgg cgtggacaag gactcgcgcg cgctcgctat cgagaagtcc
     1261 gagatcgagc gggtgcgcac ggacttcgcc gatcagcagc gcattctcga ggacgacctg
     1321 ttcctgcgcg tgcgcggcat gctcaccgga cagtccgccg ccggcggccc gcacaagctc
     1381 aaggccggca cgccgatcgc gagcgaatat ctcgatgagg tgccgcgcga ggagtggttc
     1441 gagatccgcc tggaggacga ggaggccaac tcgcagctcg agcagtccgc cgaaagactc
     1501 aagctgcagc gcaaggcctt cgagaagcgc ctcgacgata agaaagccaa gatcaccgcc
     1561 ggcgacgatc tcgcccccgg cgtgctgaag atggtcaagg tctacctcgc cgtgaagcgc
     1621 cgcgtacagc cgggcgacaa gatggccggc cggcacggca acaagggcgt gatctcgacc
     1681 atcgtgccgg tggaggacat gccctatctc gaggacggca ccccggtgga cgtggtgctg
     1741 aacccgctcg gcgtgccctc gcgcatgaac gtcggtcagg tgctggagac ccacctcggc
     1801 tgggcggcca aaggcgtcgg cctcaagatc ggccgcatgc tcgagcagca ggcggcggcc
     1861 gccgatctgc gcgccttcgt gcggcagatc tacaacgaga ccggcggcca gcgcgaggag
     1921 gtcgacagcc tcgacgatgc cgatctgacc cagctggcgc gcaacctcgc cagcggcgtg
     1981 ccgatggcga cgccggtgtt cgatggcgcg agcgaggagg agatcaagcg catgctgaag
     2041 ctcgccgatc tgccgctcaa cggtcagacc tacctgtacg acggccgcac cggcgagcgc
     2101 ttcgagcggc aggtgacggt cggctacatg tacatgctga agctcaacca tctggtcgat
     2161 gacaagatgc atgcgcgctc gaccggtccg tactcgctcg tcacccagca gccgctcggt
     2221 ggcaaggcgc agttcggcgg ccagcgcttc ggcgagatgg aggtgtgggc gctggaggcc
     2281 tacggggcgg cgtacacgtt gcaggagatg ctgacggtca agtcggacga cgtgaacggc
     2341 cgcaccaaga tgtacaagaa catcgtcgat ggcaaccatc agatggatgc gggcatgccg
     2401 gagtccttca acgtcctcgt gaaggagatc cgctcgctcg gcatcaacat ggagctggag
     2461 caggattgaa aaaccacccc acaaaccacc ccaagaaatc acgctcgacg cgatgaacga
     2521 gtcatgcacc gaccatcaag acgcgtgatt tctcggggac cccggtggaa gacggcggaa
     2581 acgtcgttag gagattagat gaaagattta ctgaagctct tcaagcagca cgctccggtc
     2641 gagcacttcg attcgatcaa gatcgggctg gcgtcgccgg agatgatccg tgcctggtcg
     2701 tacggcgagg tgaagaagcc cgagaccatc aactaccgca ccttcaagcc cgagcgcgat
     2761 ggcctgttct gcgccaagat cttcgggccg gtgaaggact acgagtgcct gtgcggcaag
     2821 tacaagcgcc tcaagcaccg cggcgtggtg tgcgagaagt gtggggtcga ggtgacccag
     2881 tccaaggtgc ggcgcgagcg catgggtcac atcgagctcg cgagcccgac cgcgcacatc
     2941 tggttcctca agtcactccc ctcgcgcatc ggcctgatgc tcgacatgac gttgcgggag
     3001 atcgagcgcg tgctgtattt cgaggccttc gtggtcatcg atccgggcat gacgccgatg
     3061 cagcgcggcc agctgctcac cgatgagatg tacctcgagg ccatcgagga gcacggcgat
     3121 gagttcgacg cgcgcatggg cgcggaagcg gtgtacgagc tgatgcgcac catcgatctc
     3181 agtgccgaga tcgccaaggt gcgcgaggag atggccggca cctcctccga gaccaagctc
     3241 aagcgcctct ccaagcggct gaagctgatc gagtcgttcg tcgagtcggg caacaagccc
     3301 gagtggatgg tcatgaccgt gctgccggtg ctgccgccgg acctgcggcc gctggtgccg
     3361 ctcgatggcg ggcgctttgc caccagcgac ctgaacgacc tctaccgccg cgtcatcaac
     3421 cgcaacaacc gcctgcgtcg tctgctcgag ctcaatgcgc ccgacatcat cgtgcgcaac
     3481 gagaagcgca tgctgcagga agcggtggat gcgctgctcg acaacggccg gcgcgggcgt
     3541 gccatcaccg gcaccaacaa gcggccgttg aagtcgctcg ccgacatgat caagggcaag
     3601 caggggcgct tccgccagaa cctgctcggc aagcgcgtcg actactcggg ccgctcggtc
     3661 atcgtggtcg gtccgcagct gcgcctgcat cagtgcggcc tgccgaagaa gatggcgctc
     3721 gagctcttca agcctttcat cttccagaag ctgcagttgc gcggtgaggc ttccaccatc
     3781 aaggccgcca agcgcctggt ggagcgcgag ggaccggagg tgtgggacat cc
//
LOCUS       DATDMP010000028         8664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1337634, whole genome shotgun sequence.
ACCESSION   DATDMP010000028 DATDMP010000000
VERSION     DATDMP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337634"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3551
                     /gene="gltB"
                     /locus_tag="VI195_00500"
     CDS             <1..3551
                     /gene="gltB"
                     /locus_tag="VI195_00500"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY6822890.1"
                     /translation="LHAMRLLMPPAWHGLDTIDPELRAFYEYYSVHMEPWDGPAGVVL
                     SDGRYALCTLDRNGLRPARYVITTNRCLTVASESGVWDYRPEEVVQQGKLGPGDMLAL
                     DLERRVLLSSRDIDQRLKTRHPYKAWLKKGVRYLESDLVDARLAAEPMDRATLSLYQK
                     MFNVSQEEREDIIRVLAQDENEAVGSMGDDTPLPVLSSRVRSLYDYFRQQFAQVTNPP
                     IDPLRESLVMSLQTQIGPECNIFLPAPEHARQVVLGSPILSQRKLRQILAIEEVTHEF
                     LDLQYDPAEGLRRAIARLCAQAESAVREGKLVLLLSDRYLVKGRIPAHALLVTGAVHQ
                     HLVKAGLRCKCNLLIETGTAREPHHFACLIGYGATAVYPYMAYQVLFEMMRKGRVKLD
                     FAARLELGRSYRAGLRKGLFKIMSKMGISTISSYRSSQLFEIVGLADEVVHLCFTGTE
                     SRVQGADFGDLEAEALALARLAWDPREAIPQGGLLKYVHGGEYHMYNPDVIAALQAAV
                     VARDPTHYREFAQLVNSRPPSTFRDLLALRPLGPPVPLAEVEPIEAVLARFDSAGMSL
                     GALSPEAHEALAIAMNRLGARSNSGEGGEDPARYRSERNSKIKQVASGRFGVTPEYLI
                     NAEVLQIKIAQGAKPGEGGQLPGHKVNDMIATLRFARPGVGLISPPPHHDIYSIEDLA
                     QLIFDLKQVNPQALVSVKLVAEPGVGTVAAGVAKAYADLITISGYDGGTGASPLSPIK
                     YAGTPWELGLAETHQTLRLNDLRHRVRLQADGGLKSGLDVIKAALIGAESFGFGTAPM
                     VALGCKYLRICHLNNCATGVATQHQLLRSKYFVGLPEMVINYFSFVAAEVREILAALG
                     ARSLEEIIGHTECLQALPGTTPKQRKLDLAPILSTAGAAADAPRFCSIARNAPFDRGE
                     QAECMVRDMLPAIAARTGGEWHYELRNYHRSIGARLSGEIARRWGNYGMEDAPLIVRC
                     RGHAGQSFGVWNAGGLHLYLEGDANDYVGKGMAGGRIVLRHPRDARFVARDTVIMGNT
                     CLYGATGGELYAAGVAGERFAVRNSGALAVIEGAGDHCCEYMTGGAVCVLGRTGVNFG
                     AGFTGGFAYVLDLERNFVDRYNHELIDISRIHPEAMQSHVQHLEALLERHLAATMSAW
                     AEEIAGDLRTFLPKFWVVKPKAASVDSLIENLRRAA"
     gene            3568..4983
                     /locus_tag="VI195_00505"
     CDS             3568..4983
                     /locus_tag="VI195_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6822891.1"
                     /translation="MTDKHLQFLELPRTEPAKAAAEARIGNFREIYASFDSAGAASQA
                     GRCLACGNPFCEWKCPVHNYIPNWLRLIEDGNLFEAAELSHKTNSLPEVCGRICPQDR
                     LCEGACTLNDGLGAVTIGAIEKYITDEALRQGWQPDMSHVQRSDRRVAIVGAGPAGLA
                     CADVLVRNGVQPVVFDRQARIGGLLTFGIPPFKLEKEVVERRRELLQGMGVEFRLECD
                     IGCDLPFAQLLADYDAVFLGMGTYRYVRGGFAGEDLPGVYEALPYLISNIRYELGFEG
                     AGHALISMRGQRVLVLGGGDTAMDCNRTAIRQGAHSVTCTYRRDESNMPGSRRDYRNS
                     REEGVEFLFNRQPIEIVGAERVQGVKLVATVLGPPDARGRRVPQPLAGSEQIVAADAV
                     IMAFGFLPSPPAWLTEHGIRVHAGGRVRVSAGANSAFQTTNPKVFAGGDMVRGSDLVV
                     TAVYEGREAARGILQYLGVRC"
     gene            complement(5534..7516)
                     /locus_tag="VI195_00510"
     CDS             complement(5534..7516)
                     /locus_tag="VI195_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6822892.1"
                     /translation="MSLRLKLLALGLATLGLPWAGCRYAREMEAALREGEQTSLQAVA
                     QTIAASLQGRTDLLFREGMPPAESAPDAPPPAPGPYDLRPLALSAAPLIDGYADDWPR
                     EPAAWAYFSHDAQHRFGVLSGVFERMLYVLLEVHDAHPVFDAPGSNWLDPATFGDRVW
                     IGYEDSHGEEQQLFLAATGPGALSARRIEAGEFGEQRVIIEPRVRAAWQAASGGYRVE
                     IRVPLSMLGERFGVLVDDRDVRGGAPVSYGTLRSEDLHTLGRLIVAAPELSSYLAQFM
                     QPGLKLAISAPDGHLLAHVDALAQARTLSTGAPLLARLYRRFIDRPGAPSLIEARAPI
                     YARDSREEIAALEVTQTPDRWLRLRDRALTRMLNFTLGTSVMAVLLMFAFAAWLALRL
                     ARLRRASESALTRTGLVTAFPDTAARDELGDVARSFSTLLGRLNAYTDYLRTLAGKLA
                     HEIRTPLTIVRSSLENLEAEPAGEAARTYLARARQGSERLNAILIAMGAASRVEEAIG
                     SAERVDFDVVALLDSAVAAYRTAFPSRLFAIELPAGPIMLHGAPDLIVQLLDKLIDNA
                     VDFSPAGATITVRLRLEGVAAILEVDNPGPPLPAPLQGRLFESLWQSRPDRDSRPHFG
                     LGLYIVRLIAEFHGGDATAVSLPGASGVRVSVRLPR"
     gene            complement(7529..8218)
                     /gene="pdsR"
                     /locus_tag="VI195_00515"
     CDS             complement(7529..8218)
                     /gene="pdsR"
                     /locus_tag="VI195_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEY6822893.1"
                     /translation="MKRLIAIVEDEAAIRDNYAAAFTREGYAVRCYANRAQAMAAFNG
                     RLPDLAVIDISLEDEPEGGFELCRELRALSAELPIIFLTARDSELDAVSGLRLGADDF
                     LTKDLSLAHLSARVNALFRRVDALRRPDTASEIVHRGALTLDAERMQAQWDGKVVLLS
                     LTEFWIVHALARHPGHVRSRQQLMDAANVVLDDNTITSHVKRIRRKFQGVDAKFDCLQ
                     TVYGMGYRWVE"
     gene            8375..8509
                     /locus_tag="VI195_00520"
     CDS             8375..8509
                     /locus_tag="VI195_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822894.1"
                     /translation="MNATSARAGFGRRLVPRCAAAGRGERLANWLALITLLALAIVWQ
                     "
BASE COUNT         1442 a         2858 c         2960 g         1404 t
ORIGIN      
        1 cgctgcacgc catgcggttg ctcatgccgc cggcctggca cggtctcgac accatcgacc
       61 cggagctgcg cgcgttctac gagtattact ccgtgcacat ggagccctgg gacggaccgg
      121 cgggagtggt gctcagcgac ggccgctacg cactgtgcac gctcgaccgc aacggactgc
      181 gtccggcgcg ctatgtgatc accaccaacc gctgcctgac cgtcgcctcc gagagcggcg
      241 tgtgggatta ccggcccgag gaggtggtgc agcagggcaa gctcggtcca ggcgacatgc
      301 tcgccctcga cctggagcgc cgtgtgctcc tgtcctcccg ggacatcgac cagcggctga
      361 agacacgcca tccctacaag gcctggctca agaagggcgt gcgctacctc gagagcgatc
      421 tggtggacgc gaggctggcg gccgagccga tggaccgtgc gacgctgtcg ctctatcaga
      481 agatgttcaa cgtcagccag gaggagcgcg aggacatcat ccgggtgctg gcacaggacg
      541 agaacgaggc ggtcggctcg atgggcgatg acacaccgct gccggtgctc tcgagcaggg
      601 tgcgctcgct ctacgactac ttccgtcagc agttcgcgca ggtcaccaat ccgccgatcg
      661 atccgctgcg cgagtcgctg gtgatgtcgc tgcagacgca gatcggaccg gagtgcaaca
      721 tcttcctgcc cgcacccgag cacgcacgtc aggtggtgct cggctcaccc atcctctcgc
      781 agcgcaagct gcggcagatc ctggcgatcg aggaggtgac gcacgagttc ctcgatctgc
      841 agtacgaccc ggccgagggc ttgcggcgcg ccattgcgcg gctgtgcgcg caggccgaga
      901 gcgcggtgcg cgaaggcaag ctggtgctgc tgctttcgga ccgctacctg gtgaaggggc
      961 gcattcccgc gcatgcgctg ctggtgaccg gtgccgtgca ccagcacctg gtgaaggcgg
     1021 gactgcgctg caagtgcaac ctgctcatcg agaccggcac cgcgcgcgag ccgcatcatt
     1081 tcgcctgcct catcggctac ggcgccacgg ccgtgtatcc ctacatggcc tatcaggtgc
     1141 tgttcgaaat gatgcgcaag gggcgcgtga agctcgactt cgccgcgcgg ctcgagctcg
     1201 ggcgcagtta tcgcgccggg ctgcgcaagg gcctgttcaa gatcatgtcg aagatgggca
     1261 tctccaccat ctcgagctat cgcagctcgc aactgttcga gatcgtcggg ctcgcggatg
     1321 aggtggtgca tctgtgcttc accggcaccg agagccgcgt gcagggcgcg gacttcggcg
     1381 atctcgaggc ggaggcgctc gcgctcgccc ggctcgcctg ggatccgcgc gaggcgatcc
     1441 cgcagggcgg cctgctcaag tacgtgcacg gcggcgagta ccacatgtac aacccggatg
     1501 tgatcgccgc cctgcaggcg gcggtagtcg cgcgcgaccc gacgcattac cgcgagttcg
     1561 cgcagctggt gaacagccgt ccgccttcga cctttcgcga cctgctcgcc ctgcggccgc
     1621 tcggtccgcc ggtgccgctc gctgaagtcg agcccatcga ggcggtgctg gcgcgcttcg
     1681 acagcgccgg catgtctctc ggcgcactct cgcccgaggc gcatgaagcg ctcgccatcg
     1741 ccatgaaccg gctcggggcg cgctccaact cgggcgaggg gggcgaggac cccgcgcgct
     1801 atcgcagtga gaggaattcg aagatcaagc aggtggcctc gggacgcttc ggtgtcacgc
     1861 ccgaatacct gatcaacgcc gaggtgctgc agatcaagat cgcgcagggc gcgaaacccg
     1921 gcgagggcgg ccagcttccg ggccacaagg tcaacgacat gattgccacg ctgcgcttcg
     1981 cgcggccggg ggtggggctg atctcaccac caccacatca tgacatctac tccatcgagg
     2041 atctggcgca gctgattttc gatttgaagc aggtgaaccc gcaggcgctg gtgtcggtga
     2101 agctggtggc cgagcccggt gtcggcacgg tcgcggccgg ggtggccaag gcgtatgcgg
     2161 acctcatcac catctccggc tacgacggcg gcactggcgc gagcccgctc tcccccatca
     2221 agtatgcggg aaccccgtgg gagctcgggc ttgccgagac tcatcagacg ctgcgcctca
     2281 acgatctgcg ccaccgcgtg cggctgcagg ccgatggcgg actgaagagc ggactcgacg
     2341 tgatcaaggc cgccctcatc ggtgccgaga gcttcggctt cggtactgcc cccatggtgg
     2401 cgctcggctg caagtacctg cgcatctgtc acctcaacaa ctgtgcgacc ggagtggcga
     2461 cgcaacatca gctgctgcgc tcgaaatact tcgtggggct cccggaaatg gtgatcaact
     2521 actttagttt cgtggccgcg gaagtacgcg agattctcgc ggcgctcggg gcgcgcagcc
     2581 tcgaggaaat catcggccac accgaatgtc tgcaggcgct acccggcacg acgccgaagc
     2641 agcgcaagct cgatctcgcg ccgatcctgt cgacggccgg ggcggcagcc gatgcgccgc
     2701 ggttctgcag catcgcccgc aacgcgccct tcgaccgcgg cgagcaggcc gagtgcatgg
     2761 tgcgcgacat gttaccggcc atcgcggcca ggaccggcgg ggaatggcac tacgagctgc
     2821 gcaactatca ccgctcgatc ggcgcccggc tgtcgggaga gatcgcgcgc cgctggggca
     2881 actatggaat ggaggacgcc ccgctcatcg ttcgctgccg cggccacgcc ggccagagct
     2941 tcggtgtatg gaatgcgggc ggcctgcatc tgtatctcga gggcgacgcc aatgactacg
     3001 tcggcaaagg catggcgggc ggcaggatcg tgctgcgcca tccgcgcgac gcacgcttcg
     3061 tcgcgcgcga caccgtcatc atgggcaaca cctgcctcta cggggccacc ggcggcgagc
     3121 tgtacgccgc cggcgtcgcc ggcgagcgtt tcgcggtgcg caactccggt gcgctggcgg
     3181 tgatcgaggg cgcgggcgat cactgctgcg agtacatgac cggcggcgcc gtgtgcgtac
     3241 tcggccgcac cggagtgaat ttcggagccg gcttcaccgg cggcttcgcc tacgtgctcg
     3301 atctcgagcg caacttcgtc gaccgctaca accacgagct catcgacatc agccgcattc
     3361 atcccgaggc catgcaatcg cacgtgcagc acctcgaggc actgctcgag cgccacctgg
     3421 cggcgaccat gagcgcctgg gccgaggaaa tcgccggcga cctgcgcacc ttcctgccaa
     3481 agttctgggt cgtgaagccc aaggccgcct cggtcgactc cctcatcgag aacctgcggc
     3541 gcgcggcctg aaggtgcgga tgcgttcatg accgacaagc acctgcaatt cctcgagctg
     3601 ccccgtaccg agccggccaa ggctgcggct gaagcgcgca tcggcaactt ccgcgagatc
     3661 tatgcgagtt tcgactccgc cggcgcggct tcccaggcgg gccgttgtct cgcctgcggc
     3721 aatccgttct gcgaatggaa atgcccggtg cacaactaca ttcccaactg gctgcgtctc
     3781 atcgaggacg ggaatctctt cgaagcggcg gagctttcgc acaagaccaa ttcgctcccg
     3841 gaggtctgcg gccgcatctg cccgcaggac cggctgtgcg aaggcgcctg cacgctcaat
     3901 gacgggctcg gcgcggtcac gatcggagcc atcgagaaat acatcaccga tgaggccctg
     3961 cggcaggggt ggcagcccga catgtcgcac gtgcagcgta gcgaccggcg cgtggcgatc
     4021 gtcggtgccg gccccgcagg gctcgcctgt gccgatgtgt tggtgcgcaa cggcgtgcag
     4081 ccggtcgtgt tcgaccgtca ggcacgtatc ggcgggctgc tcaccttcgg cattccgccc
     4141 ttcaagctcg agaaagaagt ggtggagcga cgccgcgagc tgctgcaggg catgggagtc
     4201 gagttccgtc tcgagtgcga tatcggctgc gatctgccct tcgcgcagct gctcgcagac
     4261 tacgatgccg tattcctcgg catgggcacc tacaggtatg tgcgtggcgg atttgccgga
     4321 gaggacctgc ccggagtgta cgaggcgctg ccgtatctca tttccaacat caggtacgag
     4381 ctcggcttcg agggcgccgg tcacgctctg atcagcatgc gcgggcaacg ggtgctggtg
     4441 ttggggggcg gcgataccgc catggactgc aaccgtacgg cgatccggca aggcgcgcac
     4501 agcgtcacct gcacctaccg gcgcgatgaa tccaacatgc ccggctcacg ccgcgactac
     4561 cgcaacagcc gcgaagaggg agtggagttt ctgttcaacc gccagccgat cgagatcgtg
     4621 ggcgcggagc gcgtgcaggg cgtgaaactt gtggcgaccg tcctcgggcc accggatgcg
     4681 cgcgggcggc gcgtaccgca gccgctggcc ggctccgagc agatcgtggc ggcggacgcg
     4741 gtgatcatgg ccttcggttt cctgccgagc ccaccggcct ggctcaccga gcatggcatc
     4801 cgcgtgcacg ccggcgggcg cgtgcgcgtc tcggcgggag ctaacagcgc ttttcagacc
     4861 accaacccga aagtgttcgc gggcggtgac atggtgcgtg gctcggatct ggtcgtaacc
     4921 gcggtgtatg agggacgcga ggccgcccgc ggcatcctgc aatacctcgg ggtgcgctgt
     4981 tgactcgccg cggaggtgag tgcgttggcg cccgtgccgc gttgccacgc acgagctgga
     5041 aaagaaaagg ccccgcgggt gcggggccca gtagtttggt cgcgtcgtcg agtgacccgg
     5101 tagttcgctg tcagctccgg cctgccgcgc ctcgcgtact caaacagagt gtgcgtggcg
     5161 ttttagccgg tcgcgtcatc acgagcatct actctttgcg gctgaaacga ttggagctgt
     5221 gagctccggc ttctatcgcg aggcccgcgt gttcatgcac gcgttgtgtc gttcctgaca
     5281 acccacagca agttagtggc tgccgtaccc gtgcccgcgt cgatccccga gttttctgtt
     5341 gtaagtttcc tcctacggga gcggcttgta cgcatcaacg ctcgaaagcg ctgctgagtc
     5401 gaaaccagaa tttcttttgc ggacagtgcc ttcccgcagt aattgtactg attcctgccg
     5461 ggattaccat gtcgcagatt gtcgacaaat tgctataggc agtggcgcgg acgcgacgcg
     5521 gtgggaagcg ggctcaacgc ggaaggcgca cgctcacacg gaccccggag gctcccggaa
     5581 ggctgacggc ggtggcgtcg ccaccgtgga actcggcgat gagccgaacg atgtagagac
     5641 cgagtccgaa atgcgggcgg ctgtcccgat cgggtcgcga ttgccacagg gactcgaaca
     5701 agcgcccctg caggggcgca ggcagcgggg gacctgggtt gtcgacctcg agaatcgccg
     5761 caacgccctc gaggcgcagt cgcacggtga tcgtggcacc ggcggggctg aaatcgaccg
     5821 cgttgtcgat cagcttatcg agcagctgca cgatcagatc gggagcacca tgtaacataa
     5881 tcggcccggc cggcagctcg atggcgaaca gccggctggg aaaggcggtg cggtaggccg
     5941 cgaccgccga gtcgagcagc gctaccacat cgaaatcgac gcgctcggcg ctgccgatcg
     6001 cctcttccac ccggctcgca gcacccatcg cgatgaggat cgcattcagc cgctcgctcc
     6061 cctggcgggc gcgcgcgagg taagtgcggg ccgcctcgcc cgccggctcc gcttcgaggt
     6121 tctcgagtga ggagcgcacg atggtgagcg gcgtacggat ctcgtgagcg agcttgccgg
     6181 cgagggtgcg caaataatcg gtgtacgcgt tgagtcgccc cagtagcgtc gagaagctac
     6241 gggcgacatc gccgagctca tcgcgcgcgg cggtgtcggg gaacgcggtc acgaggcccg
     6301 tgcgcgtcag cgccgactcg ctcgcccgtc gcagccgcgc gagccgcagg gcgagccacg
     6361 cggcaaaggc aaacatcagc agcactgcca tgacgctggt gccgagcgtg aaattcagca
     6421 tgcgcgtgag cgcgcgatcg cgcagccgca gccagcggtc gggtgtctgc gtgacctcga
     6481 gcgcagcgat ttcctcgcgg ctatcgcgcg cgtagatcgg agcgcgcgcc tcgatcaggc
     6541 tgggtgcacc ggggcggtcg atgaagcggc ggtagagccg ggcgaggagc ggggcgccgg
     6601 tgctgagcgt gcgcgcctgc gcgagcgcat cgacgtgcgc gagcaggtgg ccatcgggtg
     6661 cgctgatcgc gagcttcagg cccggttgca tgaactgtgc gaggtaggag gagagctcgg
     6721 gagccgccac gatgaggcgg ccgagggtgt gcagatcctc gctgcgcagg gtgccgtagc
     6781 tcacgggagc gccgccacgc acgtcgcgat cgtcgaccag taccccgaag cgctcgccga
     6841 gcatggagag cggcacacgg atctccaccc ggtagccgcc gcttgccgct tgccacgcgg
     6901 cccgtacgcg cggctcgatg atgacgcgct gctcgccgaa ttcgcccgct tcgatgcgcc
     6961 gcgcgctcag cgccccgggg ccggtcgcgg cgaggaacag ctgctgctcc tcgccgtgac
     7021 tgtcctcgta gccgatccac acgcggtcgc cgaaggtggc cgggtccagc caattgctgc
     7081 ccggcgcatc gaacaccgga tgcgcatcgt gcacctcgag cagcacatac aacatgcgct
     7141 cgaacacgcc gctcagcacg ccgaagcgat gctgcgcgtc gtggctgaaa tatgcccacg
     7201 cggcaggctc gcgcggccag tcatctgcgt acccatcgat gagaggtgcg gcgctcagcg
     7261 cgagcggccg cagatcgtag ggtcccggcg ccggcggcgg cgcatccggc gcgctctcgg
     7321 ccggcggcat cccctcacgg aagagcagat cggtgcgtcc ctggagtgag gcggctatgg
     7381 tctgcgccac cgcctgcagg gatgtctgct cgccctcgcg cagggccgcc tccatctcgc
     7441 gcgcgtagcg gcagccggcc caggggagcc cgagcgtggc gaggccgaga gcgaggagct
     7501 tgaggcgcag cgacatcgct cgagggaatt actcgaccca gcggtagccc atgccgtaga
     7561 cggtctgcag gcagtcgaac ttcgcgtcga cgccctgaaa cttgcggcgg atgcgtttca
     7621 cgtgcgaggt gatggtgttg tcgtcgagaa ccacgttggc tgcatccatg agctgctggc
     7681 gactgcgcac gtggccagga tggcgggcga gcgcgtgcac gatccagaac tcggtgagcg
     7741 acagcagcac caccttgcca tcccattgcg cctgcatgcg ctccgcatcg agcgtgagcg
     7801 cgccgcgatg cacgatctcg ctcgccgtgt cgggccggcg cagcgcatcc acgcggcgga
     7861 acagcgcgtt cacccgcgca ctgaggtgcg caagactcag atccttggtg agaaagtcat
     7921 ccgcgccgag ccgcagtccc gacacggcat cgagctcgct gtcgcgtgcg gtgaggaaaa
     7981 tgatcggcag ctccgccgac agtgcccgca gctcccggca gagctcgaaa ccgccctcgg
     8041 gctcgtcctc gagcgagatg tcgatgaccg cgagatccgg cagacgcccg ttgaaagccg
     8101 ccattgcctg agcgcggttg gcgtagcagc gcaccgcata accctcgcgc gtgaaggcgg
     8161 ccgcgtagtt gtcgcgaatg gccgcttcgt cctcgacgat ggcgatgagg cgcttcatcc
     8221 ggagtcaagg ctacaggctg tgcctgcgcc gtgaaagcgc ggcgcgccgc cactttgcgc
     8281 cacaattctt ccgcgcccgg ccacgattgc atccctgcca ggggggagct gcgcccttct
     8341 aatggcgagc tagcaaaggc tgcgggaaag ccccatgaac gcaaccagtg cgcgcgccgg
     8401 gttcggccgc cgcctcgtgc cacggtgcgc agccgcaggc cgcggcgagc ggctggccaa
     8461 ctggctggca ctcatcacgc tgctggcact cgccatcgtc tggcaatgag gatgttttcc
     8521 tcgcgcacat gaacgaagcg agctgagcgt tcagcctcgg ctttggcgcg ggccggcggc
     8581 cgattgagcc gctccgctcc ccgtgctagt ttgccgcgcg ccggcagaag aacgaattcg
     8641 cggcgacctg ctagatccga caca
//
LOCUS       DATDMP010000029         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2115563, whole genome shotgun sequence.
ACCESSION   DATDMP010000029 DATDMP010000000
VERSION     DATDMP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115563"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VI195_00525"
     CDS             complement(<1..635)
                     /locus_tag="VI195_00525"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822895.1"
                     /translation="MTPLRLAMWSGPRNISTAMMRAWENRGDCAVVDEPLYAHYLAHT
                     GLEHPGREEIIAAGEPDWRKVAARLVGPVPQGKAICYQKHMTHHLLPHIGRDWLRGLT
                     HVFLIRDPREVLNSYIRSRPQVTAEDIGVLQQLDIFEYVTQLCERTPPVIDAAEFLKA
                     PEAQLRALCAQLDIAFTPRMLSWPAGPRASDGVWAPYWYEAVLRSTGFEPYR"
     gene            complement(632..1555)
                     /locus_tag="VI195_00530"
     CDS             complement(632..1555)
                     /locus_tag="VI195_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY6822896.1"
                     /translation="MTQGTHHATDDPRNADILIYVNGELRPRAQALVSVFDSGFILGD
                     GVWEGLRVHHGRIAFLAPHLDRLYEAAKAIDLDIGLERSALTRALYQTLTANGMSDGV
                     HIRLMVTRGLKSTPYQDPRACIGPATIVIIPEYKLPKPELLTRGVRLFTVHVRRGAPD
                     VQDPKLNSHSKLNCITACIQAAKAGADEALMLDPHGFVATCNSTHFFIVRRGEVWTSS
                     GDYCIAGITRGNVLRLCRESGIPAFEKNFSLTEAYAAEEAFITGTYAGIAPVTEIDGR
                     RISPGRGPMVERLQQLYLRLIERDTAAQSAP"
     gene            1815..2876
                     /locus_tag="VI195_00535"
     CDS             1815..2876
                     /locus_tag="VI195_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6822897.1"
                     /translation="MRDANDDTVAATTGLTYPFAAAPEPGHAVGVAAGLVWLRMPLPF
                     TGLNHINLWALEDGAGWTLIDTGMQTPDTAAHWQELFAGALARRPVTRVICTHMHPDH
                     IGMAGWLTRLHACRLWTTRLEYLTCRVLVADTGREAPPDGLRFYRAAGWDEDALATYK
                     ARFGGFGKAVHVLPDSYRRIVDGEEILIGGRPWRAVIGRGHSPEHLCLHCPELQVIIS
                     GDQVLPRITSNVSVFPTEPDANPLQEWLESLASIAERVPDDVLVLPSHNEPFRGLHAR
                     LNTLISGHEQRLALMHEQLATPKRAVDLFGVLFRRRVGADMLIMATGESVAHLNCLIE
                     RQLAVRELDSTGVAWYRRA"
     gene            2927..>3607
                     /locus_tag="VI195_00540"
     CDS             2927..>3607
                     /locus_tag="VI195_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY6822898.1"
                     /translation="MTDQPLTTIAEQSGFRCTGRSEEVTRLCAAFAAAWPAAVRQLEF
                     GRSAQGRPMRALLIARGDARALPLLMLQGGIHPGESDGKDAGFMALRELLAGSAAPGV
                     LERVAILFVPAFNIDGHERCGRWNRPNQNGPEETGWRTTAHNLNLNRDYTKADSPEMR
                     ALLGLVRSWDPLVCADLHVTDGADFQPDISLQAEPINQGDPHLYAAGRELRDALIDKL
                     TAQGSMPLP"
BASE COUNT          587 a         1192 c         1193 g          635 t
ORIGIN      
        1 cggtagggct cgaagcccgt cgaacgcagc acggcctcgt accagtacgg cgcccacacg
       61 ccatcactgg cgcgcggtcc ggccggccag ctcagcatgc gcggggtaaa ggcgatatcg
      121 agttgtgcgc acagcgcacg cagctgcgcc tcgggagcct tgagaaactc ggcggcgtcg
      181 atcaccgggg gtgtgcgctc gcagagttga gtgacgtact cgaagatgtc gagctgctgc
      241 agcacgccga tatcctcggc ggtcacctgc gggcggctgc ggatgtagga attgagcacc
      301 tcacgcggat cgcggatcaa aaacacgtgc gtgagcccgc gcagccagtc gcggccgatg
      361 tgcgggagca ggtggtgcgt catgtgcttt tgatagcaaa tggccttgcc ctgcggcacc
      421 ggcccgacca ggcgcgcggc gaccttgcgc cagtccggct cacccgcggc aatgatctcc
      481 tcgcggccgg gatgctcgag gccggtgtga gccagatagt gcgcgtacaa aggctcgtcc
      541 actaccgcgc agtcgccgcg gttctcccac gcgcgcatca tcgcggtgga aatgttgcgc
      601 ggtccggacc acattgcgag ccgcagcggc gtcatggcgc agactgggcc gcggtatcgc
      661 gctcgatcag gcgcagatag agctgctgca gtctctcgac catgggtccc cggcccggac
      721 tgatgcggcg cccgtcgatc tcggtcaccg gcgcgatgcc ggcgtaggtg ccggtgatga
      781 acgcctcttc cgcggcgtag gcttcggtga gactgaagtt cttctcgaag gccgggatgc
      841 cgctctcccg gcagaggcgc agcacgttac cccgggtgat gccggcgatg cagtagtcgc
      901 cgctcgaggt ccatacctcg ccgcgcctca cgatgaagaa gtgggtcgaa ttgcaggtcg
      961 cgacgaaccc gtgcgggtcg agcatcagcg cctcatcggc tcccgccttg gctgcctgga
     1021 tacaggcggt gatgcagttg agcttggaat gcgagttgag cttgggatcc tgcacatccg
     1081 gcgcgccgcg ccgcacgtgc acggtgaaca gccgcacgcc ccgtgtcagc agctcaggtt
     1141 tcggcagctt gtactccgga atgatgacta tggtcgccgg gccgatgcac gcacgcgggt
     1201 cctggtaagg cgtgcttttc aggccccgtg tcaccatgag tcgtatgtgg acgccatcac
     1261 tcataccgtt tgccgtgagc gtctgataca gcgcgcgcgt gagtgccgac cgctccaagc
     1321 cgatatcgag atcgatcgct ttcgccgcct cgtacaggcg gtctaagtgg ggagcgagga
     1381 atgcaatgcg cccgtgatgc acgcgcaagc cttcccacac cccatcccct aggatgaaac
     1441 ccgagtcgaa caccgacacc agcgcctgcg cgcgcggacg cagctcgccg ttgacataga
     1501 tgagtatgtc ggcgttgcgc ggatcgtcgg tggcgtgatg cgtcccctgt gtcatgctgt
     1561 tcacctgctc ttagcgcagc gccggaggcg attctagcgc gagcgagcag tcctccgtcg
     1621 atgcgggcag cgccgtcgat cttactcagc cgtccgtgga ctgaatgaac ctggagccac
     1681 cggcggcgtt gatcttcgct gctgtgactg atctattcgc acgatgttac tgctcaagcg
     1741 ggtggatata ctgctcgggg tccttaagac agaatctaat gccctagtcc ggccgattcc
     1801 ctggattgag cgcgatgcgc gacgcaaacg atgacaccgt tgccgccacc acggggttga
     1861 cctacccctt cgccgctgcg cccgagccgg ggcatgccgt aggtgtcgcc gctgggctcg
     1921 tgtggctgcg catgccgctg cctttcaccg gactcaatca catcaacctc tgggcgctgg
     1981 aggacggcgc cggctggacc ctcatcgata ccggtatgca gacgccggac acggctgcgc
     2041 actggcagga gttatttgcc ggtgcgctcg cgcggcgtcc ggtcacgcgg gtcatctgca
     2101 cgcacatgca cccggaccat atcggcatgg cgggctggct gacgcggctg cacgcgtgcc
     2161 gtctctggac cacgcggctc gagtacctta cctgccgcgt gctcgtcgcc gacacgggtc
     2221 gcgaggcacc accggatggg ctgcgcttct accgtgcggc tggctgggat gaggatgcgc
     2281 tcgctaccta caaggcgcgc ttcggcggct tcggcaaggc ggtacacgtg ctgcccgaca
     2341 gctatcgccg catcgtggat ggcgaggaga tcctcattgg cgggcgtccg tggcgcgcgg
     2401 tgatcggccg cggccactcg ccggaacacc tatgcctgca ctgcccggag ctgcaggtaa
     2461 tcatctcggg cgatcaggtg ctgccgcgca tcacctccaa cgtctccgtg tttccgacgg
     2521 agcccgacgc caatccactg caggagtggc tcgagtccct cgcttcgatc gccgagcgtg
     2581 tcccggatga tgtgctggtg ctgccctcgc acaacgaacc gttccggggg ctgcatgccc
     2641 gcctcaacac actcatcagc ggacatgagc agcggctcgc tctcatgcac gagcagctcg
     2701 cgaccccgaa gcgcgccgtc gatctgttcg gcgtgctgtt tcgccgccgg gtgggtgcgg
     2761 acatgctgat catggcgacc ggagaaagcg tggcgcacct caattgcctg atcgagcggc
     2821 agctcgcggt gcgcgagctt gattccacgg gagtggcgtg gtaccgccgc gcctgaagcc
     2881 cgcgttcaac gagcgcaact ggcgcggact cccgtaaact tcgcgcatga ccgatcagcc
     2941 gctcaccacc atcgccgagc aatcgggctt tcgctgcact gggcggagtg aggaagtgac
     3001 gcggctgtgc gcggcgttcg cggccgcttg gccggctgcg gtgcgccagc tcgagttcgg
     3061 gcgctcggcg caaggccggc cgatgcgcgc tctgctcatc gcgcgcggcg atgcacgcgc
     3121 gctgccgctc ctcatgcttc agggcggtat ccaccctggc gagagcgacg gcaaggacgc
     3181 aggcttcatg gccctgcggg agctgctggc cgggagcgct gcgcccggtg tgctcgagcg
     3241 tgtggcgatc cttttcgtgc cggcgttcaa catcgacgga cacgagcgtt gcggccgctg
     3301 gaaccgcccg aaccagaacg gaccggaaga gaccggctgg cgcacgaccg cacacaacct
     3361 caatctcaac cgcgattaca ccaaagccga ctcacctgag atgcgtgccc tgctcgggct
     3421 ggtccgcagc tgggatccac tggtgtgcgc cgacctgcac gtgaccgacg gtgcggactt
     3481 ccagcccgat atctcactgc aggccgagcc tatcaaccag ggcgatccgc acctgtacgc
     3541 agccggccgt gagctgcgcg acgcgttgat tgacaagctc actgcgcagg gttcgatgcc
     3601 cctgccc
//
LOCUS       DATDMP010000030         3209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1337822, whole genome shotgun sequence.
ACCESSION   DATDMP010000030 DATDMP010000000
VERSION     DATDMP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337822"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..897
                     /gene="sufC"
                     /locus_tag="VI195_00545"
     CDS             148..897
                     /gene="sufC"
                     /locus_tag="VI195_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY6822899.1"
                     /translation="MLSIRNLAVTVGGKPILNGVNLEVGTGEVHAIMGPNGSGKSTLA
                     YVLAGRPGYTVTAGEVRYLGQDLLALAPEQRARLGFFLGFQYPVEIPGVNNVYLLKAA
                     VNALRRQQGLPEVDAFDFLALVREKMQLMHMEDSFLSRGVNEGFSGGEKKRNEILQML
                     VLEPRLALLDETDSGLDIDALKVVAAGVNSQRAPDRSCVLVTHYQRLLEYVVPDYVHV
                     LLRGRIARSGDRTLALELERRGYDFLAGEAA"
     gene            905..2197
                     /gene="sufD"
                     /locus_tag="VI195_00550"
     CDS             905..2197
                     /gene="sufD"
                     /locus_tag="VI195_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY6822900.1"
                     /translation="MSALTDRIAEDFESAARTLPGSAAAAARRRGAISALAARGLPSS
                     RDENWKYANLRSLERVRFLPAPPAQSLPSSGELPQPLPGIARYLFIDGRFAPAASATP
                     FAGAAVRLLAPRGEDAMAGAEAAATEERFALLNEAFASDGLTVDVPASSTTALQLEVL
                     FLASAAAQVGASYPRLEVRLAPGTQLTLIERHLSAAAEASFVASAVQVEIGRGARLEH
                     YRLQELNGRSILFDTLSARLDEEGMYRLHAINTGAQSARSTLSLALTGERAELALAVV
                     SLGDAHQAQDGYAVVEHAAPAARTVQTFRGIAAGRARVAFNGKIIVAPGAHGTDSRQS
                     LRGLLAGPEAEIDVRPQLEIYNDDVRCSHGATAGKLDDAMLFYLLSRGLDRDAAQRLL
                     KWAFLEDVIAKIELPPLRRQVEERLAGQLRDEALRELL"
     gene            2197..>3209
                     /locus_tag="VI195_00555"
     CDS             2197..>3209
                     /locus_tag="VI195_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6822901.1"
                     /translation="MAAKIPLENAPPPLDVEAVRRDFPILATSVHGRPLTYLDSAASS
                     QRALPVLRAVEEYERHAHANVHRGVHALSQAATAAFEGARERVRRFINAASVKEIIFV
                     RGTTEGINLVAQSYARPRLRPGDEILITGLEHHANIVPWQMVCEQTGASLRVAPIDRR
                     GEVPFEEFQRQLSPRTRLAAFAHISNALGTVLPVKRMIEAAHAQGAVVLIDGAQAVPH
                     TRVDVRALGADFYTFSGHKMYAPTGIGVLYGRAELLAAMPPWQGGGDMILTVSFEKTT
                     YNELPAKFEAGTPNISGAVGLAAAMDYLEGLGIEAVAAHEQRLLELATAELARMPAIE
                     IVG"
BASE COUNT          493 a         1118 c         1095 g          503 t
ORIGIN      
        1 cgccgcggag gacgcggtca acatgatcgt gagcggcttc tgcaagtcgg tgttcaaaga
       61 gctgccgatg gagttcgcgg tcgaggcgac caagctcctg gccgtgagcc tggaaggcgc
      121 ggtgggatga tggcgtccga cactgccatg ctgagcatca ggaaccttgc cgtgacggtg
      181 ggcggcaaac ccatcctcaa cggtgtcaac ctggaagtcg gcacgggcga ggtgcacgcc
      241 atcatgggcc cgaacggatc cggcaagagc acgctcgctt acgtgctcgc cgggcgcccc
      301 ggttacaccg tcaccgccgg tgaggtgcgt tacctcgggc aggacctgct ggcactcgcg
      361 ccggagcagc gtgcgcgcct cgggttcttt ctcggtttcc agtatccggt ggagatccca
      421 ggggtcaata acgtctacct gctcaaggcg gccgtgaacg cgctgcgccg gcagcaggga
      481 ctgccggagg tggacgcatt cgacttcctc gcgctggtgc gcgagaagat gcagctcatg
      541 cacatggagg acagcttcct ctcgcgtggc gtcaacgagg gtttctccgg cggcgagaag
      601 aagcgcaacg agatcctgca gatgttggtg ctcgagccgc gcctcgcgct gctcgatgag
      661 accgattccg ggctcgacat cgacgcgctc aaggtggtgg cggccggagt gaactcgcag
      721 cgcgcgcccg accgctcctg cgtgctcgtc acccactacc agcggctgct cgagtacgtg
      781 gtgccggact acgtgcacgt gctcttgcgc gggcgcatcg cgcgcagcgg cgatcgcacg
      841 ctcgccctag agctcgagcg ccgtggctac gacttcctcg ccggcgaggc ggcgtgagcg
      901 ccacatgagc gccctcaccg accgcatcgc cgaagacttc gagagtgccg cgcgcacgct
      961 accggggagc gccgcggccg cggcgcggcg ccgcggcgcc atcagtgcgc tcgcggcgcg
     1021 cggactgccg agctcccggg atgagaactg gaagtacgcc aatctgcgct cgctcgagcg
     1081 cgtgcgcttc ctgccggcgc cgcccgcgca gagcttgccg tcgagcggcg agctgccgca
     1141 gccgctcccg ggtatcgcgc gttacctgtt catcgatggc cgcttcgcgc cggctgcatc
     1201 ggcgacgcct ttcgccggtg cggcggtgcg gctgctcgcg ccgcgcggcg aggatgccat
     1261 ggcgggggcg gaggccgccg ctaccgagga acgcttcgcg ctcctcaacg aggccttcgc
     1321 cagcgatggc ctcacggtcg atgtgcccgc atcgagcacg acggcgttgc agctcgaggt
     1381 gctgttcctc gcaagcgcag cggcgcaagt gggcgcctcc tatccacggc tcgaggtgcg
     1441 gctagcaccc gggacgcaac tcacgctcat cgaacgacac ctgagtgccg ccgccgaggc
     1501 gagcttcgtg gcgagtgccg tgcaggtgga gatcggccgt ggcgcgcgcc tcgagcacta
     1561 ccgcctgcag gagctcaatg gccgcagcat cctcttcgac acgctttccg cacggcttga
     1621 tgaggagggc atgtaccggc tgcatgccat caacaccggt gcgcaatcgg cgcgctcgac
     1681 gctttcgctg gcgcttaccg gggagcgggc cgagctcgcg ctcgcggtgg tctcactcgg
     1741 cgacgcccac caggcgcagg atggttatgc cgtagtcgag cacgctgcgc ccgccgcgcg
     1801 caccgtgcag accttccgcg gtatcgccgc cggccgcgca cgtgtcgcct tcaacggcaa
     1861 gatcatcgtc gcgcctggcg cgcatggcac cgactcgcgc cagtcgttgc ggggactcct
     1921 ggccgggccc gaagcagaga tcgatgtgcg cccgcagctc gagatctaca acgacgacgt
     1981 gcgctgcagc cacggcgcca ccgccgggaa gctcgacgac gccatgcttt tctatctcct
     2041 gtcccggggc ctcgaccgcg atgcggcgca gcgcctgctc aaatgggcct tcctcgagga
     2101 tgtgatcgcg aagatcgaac tgcctcccct gcggcgccag gtcgaggagc gcctcgccgg
     2161 gcaattgcgt gatgaggcgc tgcgggagct gctgtaatgg ctgccaagat acctctcgag
     2221 aacgcaccgc caccgctcga tgtcgaggcg gtacggcgcg attttccgat tctcgccacg
     2281 agcgtgcacg ggcggccgct cacctatctc gacagcgccg cctcctcgca gcgggcgctg
     2341 ccggtactgc gcgcggtgga ggagtacgag cggcatgccc atgccaacgt gcatcgtggc
     2401 gtgcacgcgc tcagccaggc ggccaccgcc gccttcgaag gtgcgcgcga gcgggtacgg
     2461 cgcttcatca acgcggcctc ggtgaaggag atcattttcg tacgtggcac caccgaggga
     2521 atcaatctgg tcgcccagtc ctacgcgcgc ccgcgcctgc gacccggcga tgagatcctg
     2581 atcaccggcc tcgagcatca cgccaacatc gttccctggc agatggtgtg cgagcagacc
     2641 ggcgccagct tgcgcgtggc gccgatcgac cgccgcggcg aggtgccgtt cgaggagttc
     2701 cagcgccagc tttcgccgcg cacgcgcctc gccgccttcg cacacatttc caacgcgctc
     2761 ggcacggtac tgccggtgaa gcgcatgatc gaggccgcgc acgcgcaggg cgccgtcgtg
     2821 ctgatcgatg gggcgcaggc ggtaccgcac acgcgcgtcg acgtccgcgc gctgggtgcc
     2881 gacttctaca ccttctccgg ccacaagatg tatgcgccga ccggcatcgg cgtcctgtac
     2941 ggccgcgcgg agctgctcgc cgccatgcca ccgtggcagg gcggcggcga catgattctc
     3001 accgtgtcgt tcgagaagac cacctacaac gagctgccgg ccaagttcga ggccggcaca
     3061 cccaacatct cgggcgccgt cgggctcgcg gccgccatgg actacctcga gggtctcggc
     3121 atcgaagcgg ttgccgccca cgagcagcgg ctgctcgagc tcgccaccgc cgagctcgcg
     3181 cgcatgccgg cgatcgagat cgtgggcag
//
LOCUS       DATDMP010000031         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1226955, whole genome shotgun sequence.
ACCESSION   DATDMP010000031 DATDMP010000000
VERSION     DATDMP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226955"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VI195_00560"
     CDS             complement(<1..469)
                     /locus_tag="VI195_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822902.1"
                     /translation="MSGNGLELANNGDSLPVAANGTFTFETAVASGSHYAVTVTQQPT
                     GTPAQNCTISAASGDVGSANVTDVAVACANIARFAYIAAFNLIDAGVVWAFAIEPASG
                     ALTPVAGSPFSSGDETHSLAVHPSGRFLYAANRGGGNGANARTISVYSIDPSSG"
     gene            941..1651
                     /locus_tag="VI195_00565"
     CDS             941..1651
                     /locus_tag="VI195_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6822903.1"
                     /translation="MTDLADFPVTRKWPAQHPERLQLYSLPTPNGVKVSVMLEETGLP
                     YEPHLVDFDGNDQSSAEYRSLNPYGKIPAIIDPAGPEDEPLALFESGAILLYLADKSG
                     QFLPRAPAARYRTLQWLMFQVGAVGPMFGQVGFFHKFAGREYADKRPRDRYVAEAQRL
                     LRVLDLRLEGRAWLMGAAYTIADMAVFPWVRNLIGFYGAGELVGIGDFPQVTRALEAF
                     LARPAVARGLNIPRKAAQ"
     gene            1648..2481
                     /locus_tag="VI195_00570"
     CDS             1648..2481
                     /locus_tag="VI195_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6822904.1"
                     /translation="MSRHGDFRGKVALVTGASSGIGEELAEQLAQQGALLTLAARRTE
                     VLARLAQRIADATGSRPLVIECDVTRDGDVERAVAETVRARGKLDIVFANAGFGVAGL
                     FTGLSVADYRRQFETNVFGVLRTLYAALPEVTKTAGHLVITGSIAGWVATPGTSAYAM
                     SKFALRALANSITPELAQLGVALTLISPGFVESNIRRVDNRGTLHAETPEPMPAWLVM
                     SRRRAARQILRAVARGRREAIITVHGRVLIALERCAPWIIRAVGRRMAARGRGYRPEP
                     V"
     gene            complement(2543..>2767)
                     /locus_tag="VI195_00575"
     CDS             complement(2543..>2767)
                     /locus_tag="VI195_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY6822905.1"
                     /translation="AVRLRKQIQGEFWLALAGIASVAFGVLLAARPGVGALTILWLIG
                     AYAIAFGVIMLLLAFKVRSGVKRITHAFGT"
BASE COUNT          448 a          946 c          903 g          470 t
ORIGIN      
        1 cgccgctgga gggatcgatg ctgtacaccg aaattgtccg ggcattcgcg ccattgccgc
       61 caccacggtt ggctgcatag aggaatcgac cgctggggtg aaccgcaagt gagtgggttt
      121 catcgccgga ggaaaacggg ctgccggcca ctggcgtcaa cgcaccactc gcgggttcga
      181 tcgcaaacgc ccacactaca ccggcatcga tgagattgaa ggccgcgatg taagcaaacc
      241 gcgcaatgtt cgcgcatgcg acggccacgt cggtcacatt cgcgctcccg acatcgccgg
      301 aagccgcgct gatcgtgcag ttctgcgccg gtgtccccgt cggctgctgt gtcaccgtaa
      361 cggcatagtg gctgcctgag gcgacagctg tctcgaaggt aaacgtaccg ttcgcggcta
      421 ccggcagact gtcgccgttg ttagcaagct cgagcccatt tccgctcaaa ccgcttatcg
      481 ttccacctac cgtataggtc ggggccggtg ggctgctgct gccgccgcca ccgccgccgc
      541 agccggcggc gagcagaacc cacgcagcgg taaccgctcg agctctaact tgcgacccca
      601 tcgccgtaag caccccgact ccgctcctgg cccccaacgg tcggggtgtt ttgttgttag
      661 cccgtcagac agtccccggc gtggcaacat tctatcgcac gcaagccctt cccgcgtggc
      721 ctaagccagc gccgcagatc ctgcgcggcg agctgttaag ctggcgggcg ttgctcctcc
      781 cgcggtaggc atgacggcgc ggctgcgggg taactttggg cgtatcttga cgcaagctcg
      841 tcaaacattt cttgtcgcgt tgcggtcgat ttttgaggac tgccgggcca ttccaacttc
      901 gggtcactgc gtcagccatt gagccaatgg aggggttccc gtgaccgatc tagcggattt
      961 ccctgttacc aggaagtggc cggcgcaaca ccccgagcgg ctgcagctgt actcgctgcc
     1021 gacaccgaac ggcgtcaagg tgtcggtgat gctggaggag acgggcctgc cctacgagcc
     1081 gcacctcgtc gatttcgacg gcaacgatca gtcatccgcg gagtaccgct cgctcaaccc
     1141 ctacggcaag attccggcca tcatcgatcc ggcggggccg gaagatgagc cgctggcgct
     1201 gttcgagtcg ggcgcgatcc tgctctacct cgccgacaag agcggccagt tcctgccgcg
     1261 tgcaccggca gcgcgctacc ggactctgca gtggctcatg ttccaggtcg gcgctgtcgg
     1321 tccgatgttc ggacaggtcg gcttcttcca caaattcgcc ggtcgtgagt acgccgacaa
     1381 gcgcccgcgc gatcgttatg tcgccgaagc gcagcgcctg ctgcgcgtgc tcgacctgcg
     1441 cctcgagggg cgcgcgtggc tgatgggtgc ggcctacacc atcgccgaca tggcggtatt
     1501 tccctgggtg cgcaatctca tcggctttta cggcgcggga gagctggtcg gcatcggtga
     1561 tttcccgcag gtcacgcggg cgctcgaggc gttcctggca cggccggcgg tggcgcgcgg
     1621 cctcaacatt ccacgcaaag cagcgcagtg agccggcacg gcgattttcg cggcaaggtg
     1681 gcgctggtca cgggtgcatc ctccggcatc ggtgaggagc ttgccgagca gctcgcccag
     1741 cagggcgcac tgctcaccct cgccgcgcgc cgcaccgagg tgctggcgcg ccttgcgcag
     1801 cgcatcgcgg atgccaccgg ctcgcgacca ctcgtaatcg agtgcgatgt cacgcgggat
     1861 ggcgatgtcg agcgtgccgt ggcggaaacc gtgcgcgcgc gcggcaagct cgacatcgtc
     1921 ttcgccaacg ccggtttcgg ggtggccgga ctgttcaccg ggctctcggt ggccgactac
     1981 cgccggcaat tcgagaccaa cgttttcggc gtgctgcgca cgctctatgc ggcgctgccc
     2041 gaagtcacca agaccgcggg ccatctggtg atcaccggca gcattgccgg ttgggtggca
     2101 acgccgggca cctcggctta cgccatgagc aagttcgcgc tgcgcgcgct cgctaactcg
     2161 atcaccccgg agctcgccca gctgggagtc gcgctcaccc tcatcagccc gggcttcgtc
     2221 gagagcaaca tccggcgtgt cgacaaccgt ggcacgctcc atgccgagac gcccgagccg
     2281 atgccggcat ggctcgtgat gagccgccgc agagccgccc ggcagatcct gcgcgccgtg
     2341 gcccgcgggc gccgcgaagc catcatcacc gtccacggcc gggtgctcat cgcgctggag
     2401 cggtgcgcgc cctggatcat ccgggcggtc ggcagaagga tggcggcgcg cggacgcggc
     2461 taccgcccgg agcccgtctg agatcgcggt ccggatacgc acggctcacg cccgtcgtga
     2521 gccgtgcgta ccgagcgagc gcctaggtcc cgaacgcgtg cgtgatgcgc ttcacgccgc
     2581 tgcgcacctt gaaggcgagc agcagcatga tgacgccgaa ggcgatcgca taggccccga
     2641 tgagccacag gatcgtcagc gctcccaccc cggggcgggc cgccagcaac acgccgaagg
     2701 ccaccgatgc gattccggcg agtgccagcc agaactcacc ctggatctgc ttgcgcagcc
     2761 gcaccgc
//
LOCUS       DATDMP010000032         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1337963, whole genome shotgun sequence.
ACCESSION   DATDMP010000032 DATDMP010000000
VERSION     DATDMP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337963"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VI195_00580"
     CDS             <1..1536
                     /locus_tag="VI195_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY6822906.1"
                     /translation="GRPLIVALNMSDVAAQRGYQLDRAALERALGVPVIPTVAVRTGG
                     ERALVEAIDAYGFAERALSEQRAPLAAPPPPTAAEIEATQREVRRVLSDSGYRVPARV
                     KALTRLDAIVLHPLAGPLLLGSVLFLMFQAVFSWAKGPQNLINSGVQHLNAVLTGVLP
                     EGPLRGLLLDGMVAGVGSVLVFLPQILILFLFILALEDSGYLPRAAFMLDRLMGRVGL
                     SGRAFIPLLSSFACAIPGIMATRTIPSARDRLATIMIAPLMTCSARLPVYALLISAFI
                     PQRAIGVFNLRGLVLFALYIAGIASALGVAFALKRTVMRGEYRPLLLELPEYRLPHLS
                     NLLLGLWERTRIFLTRVGTIILSLMVVLWFLASFPAPPAGATGPAIQYSIAGLLGRGL
                     QFLFAPIGFNWQISIALVPGLAAREVAVSALGTVYAMSGSSDVAGALTPVIARGWSLA
                     TGLSLLAWYVFAPQCMSTLAVVKRETNSWRYPLYMAGYLFALAYLGAFVTYRAALALG
                     GAT"
     gene            1533..1850
                     /locus_tag="VI195_00585"
     CDS             1533..1850
                     /locus_tag="VI195_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822907.1"
                     /translation="MNAAHAGIALLLQQLLVGVLVISSALFSAWRLSSVRLRLRALEM
                     LAAWPGCARASWLARLRERTLAQQLRACGGCGNATRPAGQSIDRPTQGADAPNRTPGA
                     LRR"
     gene            complement(1798..2319)
                     /locus_tag="VI195_00590"
     CDS             complement(1798..2319)
                     /locus_tag="VI195_00590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6822908.1"
                     /translation="MAAVSVSVRDARSAPADRLWIQSVYRDYLDDLNPGTGIFPALGE
                     VGHREPDQIAHWFGDPNTYPLVIVKGSDPVGFARVLRAVAGGAAPRIDYRMAEFFVLR
                     TRRRLGIGQTAVQLILSRFAGRWEINEYLRNTAAVDFWRRVVASYTRGSYQERIVNGE
                     VRQVFDSVRPRPA"
BASE COUNT          347 a          865 c          869 g          483 t
ORIGIN      
        1 ggccgtccgc tgatcgtcgc actcaacatg agcgacgtcg ctgcgcagcg cggctaccag
       61 ctcgaccgcg cagccctcga gcgtgcgctc ggggtgcccg tgataccgac ggtagcagtg
      121 cgcaccggtg gcgagcgcgc actggtcgaa gcgatcgacg cctatggttt cgcggagcgc
      181 gctctcagtg agcagcgtgc gccgctcgca gcgccgccgc cgccgaccgc cgcggaaatc
      241 gaggcgacgc agcgcgaagt gcggcgggtg ctgagcgaca gcggctatcg cgtgccggct
      301 cgggtcaagg cgctcacacg actcgatgcc atcgtgctgc atccgctggc gggaccgctg
      361 ctgctcggct cggtgctgtt cctcatgttt caggcggtgt tcagctgggc gaagggaccg
      421 cagaacctga tcaactccgg ggtgcaacac ctcaacgccg tgctcaccgg cgtgctgccc
      481 gagggtccgc tgcgaggtct gctgctcgat ggcatggtcg ccggcgtcgg cagcgtgctg
      541 gtgttcctgc cgcagatcct cattctgttc ctgttcatcc tggcgctcga ggattccggt
      601 tatctgccgc gcgcggcgtt catgctcgat cggctgatgg gccgggtggg gttgtcggga
      661 cgtgcgttca ttccgctgct ctcgagcttc gcctgtgcga tcccggggat catggcgacg
      721 cgcacgatcc cgtccgcgcg tgaccgcctg gccaccatca tgatcgcgcc actgatgacc
      781 tgctcggcgc ggctgccggt gtacgcgctg ctgatcagcg ccttcatccc gcagcgtgcg
      841 atcggggtct tcaacctgcg cggactggtg ctgttcgcgc tgtacatcgc aggcatcgcc
      901 agcgcactcg gcgtcgcttt tgccctcaag cgtaccgtga tgcgcggtga gtaccggccg
      961 ctactgctcg agctgcccga gtatcggctg ccacacctgt cgaacctgct gctcggactg
     1021 tgggagcgca cgcgcatctt tctgacgcgc gtcggcacca tcatcctctc gctgatggtg
     1081 gtgctgtggt tcctggcgag cttcccggct ccgcccgcgg gcgcgaccgg gcccgccatc
     1141 cagtacagca ttgcggggct gttgggacgt gggctgcagt tcctgttcgc ccccatcggc
     1201 ttcaactggc agatctcgat cgcgctcgtg ccggggctgg cggcgcgcga ggtggctgtg
     1261 agcgcgctcg gcacggtgta cgcgatgtcc ggctcgagtg atgttgccgg cgcactgact
     1321 ccggtgatcg cacgcggctg gagtctcgcc acggggctgt cgctgctcgc ctggtacgtg
     1381 tttgcgccac agtgcatgtc gacactcgcg gtggtgaagc gcgagaccaa ctcctggcgc
     1441 tatccgctct acatggcggg ctacctgttc gcgctcgcgt atctgggtgc gttcgtgacg
     1501 taccgcgcgg cgcttgcgct cgggggcgct acgtgaacgc cgcccacgcg gggatcgctc
     1561 tgctgctgca acagctgctg gtgggcgtac tggtgatcag cagcgcactc ttcagcgcct
     1621 ggcgcctgtc ttcggtacgc ctcaggctgc gcgcgctgga gatgctcgct gcctggccgg
     1681 gctgcgcgcg cgcttcctgg cttgcccggc tgcgcgaacg cacgctcgct cagcagttgc
     1741 gcgcctgcgg gggctgcggc aacgccacgc ggccggccgg tcagtccatc gatcgtccta
     1801 cgcagggcgc ggacgcaccg aatcgaacac ctggcgcact tcgccgttga cgatacgctc
     1861 ctggtaggag ccgcgggtgt agctggcgac cacccggcgc cagaagtcga cagccgccgt
     1921 gttgcgcagg tactcattga tctcccagcg cccggcgaag cggctgagga tgagttgcac
     1981 cgcagtctgg ccgataccga gccggcgccg cgtgcgcagc acgaaaaact ccgccatgcg
     2041 atagtcgatg cgcggcgccg cgcctccggc aaccgcgcgc agcacgcgtg caaagccgac
     2101 cgggtcgctg cccttcacga tgacgagcgg ataggtgttg ggatcgccga accagtgtgc
     2161 gatctggtcg ggctcgcgat gcccgacttc gccgagggcc gggaagatcc cggtgccggg
     2221 attgagatca tcgaggtagt cgcgatagac gctctgtatc cacagccggt cggcgggcgc
     2281 cgatcgcgca tcgcgcacac tgacactcac tgcagccatg gttcaattcc ctgcttcgaa
     2341 cgttacgcgt gctgcgtagc tttcgtcacc ttaggttttt gtcatctccc atcggcaggc
     2401 cacgctgccc aaatgaaatc ggcccggacg gctcgcgcca ggcgaaccgt ccaggccgta
     2461 ttcttcagcc tgagcgcgac ttattgcttc ggcggctcag ccggcggcgt cgccggtgca
     2521 gtgccttctg ccgacttggc agcgtctgcg gcggaaccgg cagc
//
LOCUS       DATDMP010000033         4925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1115780, whole genome shotgun sequence.
ACCESSION   DATDMP010000033 DATDMP010000000
VERSION     DATDMP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115780"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..677)
                     /locus_tag="VI195_00595"
     CDS             complement(90..677)
                     /locus_tag="VI195_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822909.1"
                     /translation="MTRAPAAFDPALAPGARPLALLPPGALAARLEAALRAGEGAVGA
                     HCIHELWMRGEFPARIESALEALWRRAAASVPEWLPMRYIEWLPAAYEVALRFRAARA
                     GRTNVYLVLLDFADRRRGPYGVYVGMSRYAPALRFEQHKAGIRAAGSVLKRGLEVLTG
                     PTLHLQRIARPEAARIEAELAAALSDAGLKVQGGH"
     gene            complement(674..1942)
                     /locus_tag="VI195_00600"
     CDS             complement(674..1942)
                     /locus_tag="VI195_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6822910.1"
                     /translation="MGTSVPTQRAAVRALGVLTAINLLNYLDRYVVSAIAPDLKQAMA
                     LSDTQLGWLMPIFMVVYMVAAPGFGTWGDRGSRTRPIALGVFVWSLATLLSGFARSFP
                     QLLLGRALVGIGEAAYVAIAPALLADCFAVDRRGRVYAVLNMAIPVGAALGYIVGGAV
                     SVHFGWRAAFFVAGVPGLALALWVLRLPDPPRGVQDATANAPAAVPRAPGVPPGPVAV
                     YLGLLGRWPYLLVVLGYAAYTFGLGGLAFWMPTFLERMRDVPAETATIGFGKIVLATG
                     FLGTFAGGWLGDRCLRFSRQAYLWFAGVVTLLAAPCALLALTATAPGVYFPAIVAAEL
                     LLFMSTGPINAAIVNIAAPFERASAIALSMFAIHLLGDVPSPLLIGYLSDLSSLGRAV
                     LIVPLAVAASGAVWLISAGVSARVPRAART"
     gene            complement(2035..2820)
                     /locus_tag="VI195_00605"
     CDS             complement(2035..2820)
                     /locus_tag="VI195_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6822911.1"
                     /translation="MNVIIVDDEPAARRAVRECCEREPDLQVLGEYGDGRSALEAIRA
                     RSPDVLFLDIQMDSMTGMALARSLDPQRLPLIVFVTAFDHYALDAFEVSAVDYLLKPF
                     DEARFRATVERVRRRHLAQNGADRRAALDALLEQLERSARVRAEAQPRLLAESGSRMH
                     MLDVSQVELVEADRNYVRLTIGRETFHARSTLAHAEKSFQTQPMLRISRSCLVNMTHV
                     REVSRTPRGDFILVVAGGTTVTSSEGFREPVRQYLERLKLAPA"
     gene            complement(2817..4058)
                     /locus_tag="VI195_00610"
     CDS             complement(2817..4058)
                     /locus_tag="VI195_00610"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6822912.1"
                     /translation="MSASPLERHAAAFPADIGRDAVVAERQAGTRHSLWPQLAVLTAF
                     WIYVALSNLMYANTMGQSLRALNVTDFFAPWDARLIQHLILYPLFILCVWASLRIGWR
                     PLWRTAPIQLSCAIGFAVLAAPALATGEYLVGEAHGGHAKDMTSGMDWAEFLSGPSVS
                     TWIASATSWLVTYGFGLALITGFTFYQRLRDSQLRSAALERALTAAHLAALRMQLSPH
                     TLFNLLHTIRGQIAWDPPAAQAMVVQLGDLLRRLLSAGEREFTRLADELQFVQLYLEL
                     QQRRFADRLRVTVPARDAAPPVWVPSLILQPLVENAVVHGLAGHDGPVEVRVAARLDG
                     ETLVLRVENNLAPSWQGGGGTGGIGLGNVRERLAVQFGERASFSAGPGTDNVWFAEVR
                     LPLLRDGPDEVARPATAGTAR"
     gene            4335..4697
                     /locus_tag="VI195_00615"
     CDS             4335..4697
                     /locus_tag="VI195_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY6822913.1"
                     /translation="MTSATVTAGTRRRASATLAALTACLAAGAFATARAAPVSADPFV
                     TVSYHDLNIATDQGSQVLYARIVSAARQVCAADDVRDLRAVAAAADCRARAVAQAVRA
                     VNSPALAAVHAAALQRHG"
     gene            complement(4739..>4925)
                     /locus_tag="VI195_00620"
     CDS             complement(4739..>4925)
                     /locus_tag="VI195_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6822914.1"
                     /translation="PALEAIAGAHGVSVMQVALAWLLAKSPVMLPIPGTSSVAHLEED
                     MAAGRIRLSPAELASLG"
BASE COUNT          796 a         1757 c         1775 g          597 t
ORIGIN      
        1 tggatggtgc acgccttcga gaggacgacc atctccttca acgcgatggt catcgacagc
       61 aacgcgcacc tggggctgac cgtcaagttc taatgcccgc cctgcacctt gaggccggca
      121 tcgctcaacg ccgcggcgag ctccgcttcg attcgcgctg cctccgggcg cgcaatccgc
      181 tgcaggtgca gggtcggtcc ggtgagcacc tccagcccgc gcttgagcac gctgccggcg
      241 gcgcggatgc cggccttgtg ctgctcgaag cgcagtgccg gcgcgtagcg gctcatgccg
      301 acgtagacgc cgtagggccc gcggcggcgg tcggcgaaat cgagcagcac caggtagacg
      361 ttggtgcgtc cggcgcgcgc cgcgcggaag cgcagcgcca cctcataagc cgccggcagc
      421 cactcgatgt aacgcatcgg cagccactcg ggtaccgacg ccgcggcacg gcgccacagg
      481 gcctcgagcg cgctctcgat gcgcgcgggg aattccccgc gcatccacag ttcgtggatg
      541 caatgcgcgc cgaccgcccc ttcacccgcg cgcagcgccg cctccagacg cgccgccagc
      601 gcccccgggg gcagcagcgc gagcggccgc gcgcctgggg cgagcgccgg atcgaaggcg
      661 gccggggccc gcgtcacgtc cgcgccgcgc gcggcacgcg ggcgctcacc cccgcggaaa
      721 tcagccacac ggccccggag gcggccacgg cgagcggcac gatcagcacc gcccggccga
      781 gcgagctcag gtcggagagg tagccgatca gcagcggcga cggcacgtcg cccagcaggt
      841 gtatcgcgaa catgctgagc gcgatcgccg aggcgcgctc gaagggcgcc gcgatgttga
      901 cgatcgcggc gttgatcggg ccggtcgaca tgaacagcag gagctcggcg gcgacgatcg
      961 ccggaaagta gacgccgggt gccgtggcgg tgagcgcgag cagcgcgcac ggcgccgcga
     1021 gcagcgtgac cacgccggcg aaccacagat acgcctggcg cgagaagcgc aggcagcgat
     1081 cgccgagcca gccgccggcg aaggtgccga gaaagcccgt agcgagcacg atcttgccga
     1141 agccgatggt ggcggtctcc gccggcacat cgcgcatccg ctcgaggaag gtcggcatcc
     1201 agaaggcgag cccgccgagc ccgaaggtgt aggcggcgta accgagcacg acgagcagat
     1261 agggccagcg cccgaggagc ccgaggtaca cggccaccgg cccgggcggc acgccgggcg
     1321 cgcgcggcac cgcggccggg gcgttcgcgg tcgcatcctg cacgccgcgg ggaggatcgg
     1381 gcagccgcag cacccacagg gcgagcgcga ggcccggcac gcccgccacg aagaaggccg
     1441 cacgccagcc gaagtgcacg ctgaccgctc cgccgacgat gtaaccgagt gccgccccga
     1501 ccgggatcgc catgttgagc accgcgtaca cgcggccgcg gcgatcgacc gcgaagcaat
     1561 ccgcgagcag cgccggcgcg atggccacgt aggcggcctc gccgatgccg accagcgcgc
     1621 gcccgaggag cagctgcggg aagctccggg cgaagccgga gagcagcgtc gccaggctcc
     1681 acacgaacac cccgagcgcg atcgggcggg tgcgcgagcc gcgatcgccc caggtgccga
     1741 atcccggcgc cgcgaccatg tacaccacca tgaagatcgg catcagccag ccgagctggg
     1801 tgtccgagag cgccatcgcc tgcttgagat ccggcgcgat cgccgacacg acgtagcggt
     1861 cgagataatt gagcaggttg atggcggtca gtacgccgag cgcccggacg gcggcgcgct
     1921 gcgtcggtac ggaagtgccc aaggtgcgaa ctcgcgcgag cggcatgcgt ggcgcgcgac
     1981 tttagcgcct ttgggtggcc ggccgcgagt tgccccgggg gtggcgccag ccgctcaggc
     2041 gggcgcgagc ttgaggcgct cgaggtactg gcgcaccggc tcgcggaagc cctcgctgct
     2101 ggtcacggtg gtgccgccgg cgaccaccag gatgaaatcc ccgcgcggcg tgcggctcac
     2161 ttccctcacg tgggtcatgt tcaccaggca cgagcggctg atgcgcagca tcggctgggt
     2221 ctggaacgac ttctcggcat gtgcgagcgt gctgcgcgcg tgaaaggtct cgcggccgat
     2281 cgtgagccgc acgtagttgc gatccgcctc gaccagctcg acctgcgaca cgtcgagcat
     2341 gtgcatgcgg ctgccggact cggccagcag gcgcggctgg gcctcggcgc gcacccgcgc
     2401 gctgcgctcg agctgctcga gcagcgcatc gagcgccgcg cggcggtcgg cgccgttctg
     2461 cgccaggtga cggcgccgca cgcgctccac cgtggcgcga aaacgcgcct cgtcgaaggg
     2521 cttgagaaga tagtcgaccg cgctcacctc gaaggcatcg agcgcgtagt ggtcgaaggc
     2581 ggtgacgaat acgatcagcg gcagccgttg cggatcgagc gagcgcgcca gcgccatgcc
     2641 ggtcatcgag tccatctgga tgtcaaggaa cagcacatcc ggggagcggg cgcggatcgc
     2701 ctcgagcgcg ctgcgtccgt cgccatactc gcccagcacc tgcaggtcgg gctcgcgctc
     2761 gcagcactcg cgcaccgccc ggcgcgccgc cggctcgtcg tcgacgatga tgacgttcat
     2821 cgggccgtcc cggccgtcgc ggggcgcgcc acttcgtccg ggccgtcgcg cagcagcggc
     2881 agccgaactt ccgcaaacca gacgttgtcg gttccgggac cggcgctgaa gctggcgcgc
     2941 tcgccgaact gcaccgcgag gcgctcgcgc acgttgccga ggccgatccc gccggtgccg
     3001 ccgccgccct gccaggacgg cgcgagattg ttctcgacgc gcagcaccag cgtctcgcca
     3061 tcgaggcgcg ccgcgacgcg cacctcgacc ggaccgtcgt gcccggcgag cccgtgcacc
     3121 acggcattct cgaccagcgg ctgcaggatg aggctcggca cccacaccgg cggggccgcg
     3181 tcgcgcgcgg gcaccgtgac ccggaggcgg tcggcgaagc gccgctgctg caactccagg
     3241 tacagctgca cgaactgcag ctcgtcggcg aggcgggtga attcacgctc gccggcgctc
     3301 agcagccgcc gcagcagatc gccgagctgc accaccatcg cctgcgccgc cggcggatcc
     3361 caggcgatct gcccgcggat ggtgtgcagc aggttgaaca gtgtatgcgg cgagagctgc
     3421 atgcgcagcg ccgccaggtg cgccgcggtg agcgcacgtt cgagcgcggc ggagcgcagc
     3481 tgcgagtcgc gcagccgctg atagaaggtg aagccggtga tcagcgccag accgaagccg
     3541 taggtcacca gccagctggt cgcgcttgcg atccaggtcg agacgctcgg cccggagagg
     3601 aactcggccc agtccatgcc tgaggtcatg tccttggcgt ggccgccgtg cgcctcgccg
     3661 accaggtact cgccggtggc gagcgccggc gcggcgagca ccgcgaagcc gatcgcgcat
     3721 gacagctgga tcggcgccgt gcgccacagc ggccgccagc cgatgcgcag cgacgcccac
     3781 acacacagga tgaacagcgg atagaggatc agatgctgaa tgagacgcgc gtcccatggc
     3841 gcgaagaagt cggtgacgtt gagcgcccgc aggctctgac ccatggtgtt tgcatacatg
     3901 aggttcgaga gcgccacgta gatccagaac gcggtgagca ccgcgagctg cggccagaga
     3961 ctgtggcgcg tgccggcctg tcgttcggcg acgacagcat cgcggccgat gtcggcgggg
     4021 aacgcggcag cgtggcgttc cagcggggag gcgctcatgc cggcagcata acccagcgcc
     4081 ggaacgccgc gtgtggcagg cgctgaacgc gcgggacgag cgcgtcgatt cgcgggacga
     4141 atccgcacgg cgcgccgcca agcgacgggt gggacgttcg ccgcgcctcg cggtcaaccc
     4201 tgcaacgccc ggcgggcggt accaacgcgt cagccgccgg gatcagtccg tagcgcagcg
     4261 gctttaaacg ccgtgaccga actgcgaatc tgactgtcag cgtcgcgatg tccgtggcgc
     4321 aacaggagaa gctcatgacc tcagcaacag taaccgccgg aacgcgccgc cgcgcctccg
     4381 caacccttgc tgctctgacg gcctgtctgg ccgcgggcgc cttcgcaacc gcgcgcgcag
     4441 cacccgtctc cgcggacccg ttcgtcacgg tgagctacca cgacctcaac atcgccaccg
     4501 accagggctc gcaggtgctc tatgcccgca tcgtatcggc ggctcgtcag gtgtgcgcgg
     4561 cggatgacgt ccgcgacctg cgcgccgtcg ccgccgccgc cgattgccgc gcgcgcgccg
     4621 tcgcccaggc ggtgcgggcg gttaacagcc cggcgctcgc cgcggtgcac gccgctgcac
     4681 tgcagaggca tggctagcag cattcccagg gaaggcgtat ccgggcgcgc ccgggggctc
     4741 agccgaggct cgcgagctcg gccgggctga ggcggatgcg ccccgcggcc atgtcctcct
     4801 cgaggtgcgc gaccgaggaa gtgccgggaa tcggcagcat caccggagac ttcgccagca
     4861 gccacgccag cgcaacctgc atgacactca cgccgtgcgc gccggcgatc gcctcgagcg
     4921 ccggg
//
LOCUS       DATDMP010000034         4796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2004807, whole genome shotgun sequence.
ACCESSION   DATDMP010000034 DATDMP010000000
VERSION     DATDMP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004807"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="mnmA"
                     /locus_tag="VI195_00625"
                     /gene_synonym="trmU"
     CDS             complement(<1..705)
                     /gene="mnmA"
                     /locus_tag="VI195_00625"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY6822915.1"
                     /translation="MPAVTPPQARSARVIVGLSGGVDSAVAALLLRDAGWEVHGLFLS
                     NWEENDDAYCTAAQDFQDARAVAAELGIVLHRVSFAAAYRERVFAHFLREHRAGRTPN
                     PDVLCNREIKFGVALEWARRLGATHFATGHYARLESATDGPALCKARDGGKDQSYFLH
                     AVAQRELAATLMPLGELHKSEVRERARRAGLPVFDKRDSTGICFIGERPFREFLEQYI
                     GHTPGPVRTDAGEEIGT"
     gene            complement(741..1271)
                     /locus_tag="VI195_00630"
     CDS             complement(741..1271)
                     /locus_tag="VI195_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6822916.1"
                     /translation="MGRKPDVTVAAIAERDGRFLVVEERINRRLLFNQPAGHVERGET
                     LLAAVVREAREETAWRFDPQTLLGIYLWRNPNTGREILRFAFAGPVADHDPAQRLDRG
                     IIATHWLTPDELAARQSRLRSPLVLRCVHDFLAGQRHALDGIGQLDLECAAELGQKAT
                     AVAAGTIDAPTDTRAR"
     gene            complement(1293..1853)
                     /locus_tag="VI195_00635"
     CDS             complement(1293..1853)
                     /locus_tag="VI195_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822917.1"
                     /translation="MAQLANARYAPESANGAGGAASSRQGAETLTVLTSLAEVRAAVN
                     EIAATAQRLISIYTPDLEPDLYDQTAFLDVIKHFVLTRSFSKVRVLLAEPTRVMRDSN
                     RFVAMGRRLSSCIDIRYVAAQSPQRASSYLIADDRAIVYRMRADTWDGVADINNPAVA
                     RVYLQEFDQVWNASAAEHGLRAARRG"
     gene            complement(2072..3454)
                     /gene="purB"
                     /locus_tag="VI195_00640"
     CDS             complement(2072..3454)
                     /gene="purB"
                     /locus_tag="VI195_00640"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY6822918.1"
                     /translation="MSGSPSDCLPALEALSPVDGRYRAATEPLRALLSEAGLVRERIR
                     IEAQWLLQLAAAAPQLAGSTLALAVSECARQLAQAPDPGAAAAVKSIEARINHDVKAV
                     EYYVRERLAAAGAQAATLELVHFGCTSEDINNLSYACLLRAARGALLPVLAARTHELT
                     QLAHRYADTAMPARTHGQPASPTTLGKELANFAARLARAQRRWAAVSILGKWNGAVGN
                     FNAHIAALPALDWPALAEAFVTSLGLDYNPWTTQIEPHDWIGEYCDAVAAVNVVCIGL
                     CRDLWGYVSLGYLRQRALAGEVGSSTMPHKVNPIDFENAEGNCGIANALLRHFAEKLP
                     VSRWQRDLTDSTVLRNLGVALAHSLIAWRALGRGLEKIEADPARMSADLAAGWEVLGE
                     AVQTVLRAAGVPDGYERLKEFTRGRQIDAATLAAFIDALPLAASDKARLRSLRPESYI
                     GLAAQLARQI"
     gene            3514..>4796
                     /locus_tag="VI195_00645"
     CDS             3514..>4796
                     /locus_tag="VI195_00645"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY6822919.1"
                     /translation="MTRSFRTERDSMGELKVPTEALWGAQTQRAIENFPISGGSMPRE
                     FIAALALVKQAAARANRGLKLLDAPLAQAIDAAAAEVVAGAHNAHFPLDVFQTGSGTS
                     TNMNANEVIAALASRHLGKAVHPNDHVNMGQSSNDAIPTAIHVSAALALRRELLPALE
                     HLSDVLREKERDIGAIVKTGRTHLMDAMPLTFGQELSGWRTQVEHGVARLRAVEPRLL
                     ALAQGGTAVGTGINTHADFGRRFCQELSSLTAVPFVPAENYFEALSAQDTAVELSGQL
                     KVIAVSLIKMANDLRWMNSGPLAGLGEIALPALQPGSSIMPGKVNPVIPEATMMVGAQ
                     VIGNDTTITLAGQSGNFQLNVMLPVIAHNLLESIRLLANVSRALADSAIAGLRVNATR
                     IAAALDRNPILVTALNPVIGYEKGAAIAKKAYAEGR"
BASE COUNT          787 a         1604 c         1646 g          759 t
ORIGIN      
        1 cgtgccgatt tcctcaccgg catcggtgcg caccggtcct ggtgtgtgac cgatgtactg
       61 ctcgagaaat tcgcggaaag gccgctcgcc gatgaagcag atgccggtgc tgtcgcgctt
      121 gtcgaacacc ggcagaccgg cacggcgcgc acgctcccgc acctcgctct tgtgcagctc
      181 gccgagcggc atgagggttg ccgccagctc gcgctgtgcg accgcgtgca ggaaatagct
      241 ctggtccttg cccccatcgc gcgccttgca cagtgcgggg ccgtcggtgg ccgactcgag
      301 gcgcgcgtag tgacccgtcg cgaagtgcgt tgcgccgagc cgccgcgccc actcgagcgc
      361 cacgccgaac ttgatctcgc ggttgcagag cacatcggga ttgggggtgc ggccggcgcg
      421 atgctcgcgc aggaaatgcg cgaacacccg ctcgcggtat gcggccgcga agctcacccg
      481 gtgcagaacg atgccgagct ccgcggcaac cgcgcgcgcg tcctggaagt cctgcgcagc
      541 cgtgcagtag gcgtcgtcgt tctcctccca gttgctgaga aagaggccgt gcacttccca
      601 gcccgcatcc ctcagcagta gggctgcgac cgccgagtcc acgcccccgg agagaccgac
      661 aatgacgcgg gcggagcggg cctgaggtgg agtgactgcg ggcatcggcc aattgtaatg
      721 cgcccgctgc ggcggcgctt tcagcgcgcg cgggtatcag tcggcgcgtc gatagtccct
      781 gcggccactg cggtagcctt ctgacccagc tcggccgcgc attcgagatc gagctggccg
      841 atgccgtcca gcgcgtgacg ctgaccggcg aggaaatcgt gcacacagcg caggacgagc
      901 ggactgcgca gccgcgactg ccgtgccgcg agctcatctg gggtgagcca gtgtgtagcg
      961 atgatgccgc gatcgagtcg ctgcgccggg tcgtggtccg ctaccggtcc ggcgaacgca
     1021 aagcgcagga tctcgcggcc ggtgttggga ttgcgccaga ggtagatgcc gagcagcgtt
     1081 tgcgggtcga agcgccaggc cgtctcctcg cgtgcctcgc gcaccaccgc ggcgagcaag
     1141 gtctcgccgc gctcgacgtg tccggccggt tgattgaaga gcaggcggcg gttgatgcgc
     1201 tcctcaacca ccaggaaacg accgtctctt tccgcgatcg ccgcaacggt gacatcaggc
     1261 ttcctgccca tgtcgcggat tgtaacggcg actcagcccc ggcgcgcagc cctcaggcca
     1321 tgctcggcgg cgctcgcatt ccagacctgg tcgaattcct gcaggtacac ccgggcgacg
     1381 gccggattgt tgatgtcggc caccccgtcc caggtgtccg cccgcatgcg gtagacgatc
     1441 gcgcggtcat cagcgatgag atagctcgag gcacgttgcg gactctgcgc agccacgtag
     1501 cggatgtcga tacagctcga cagccgccgt cccatggcga cgaagcggtt gctgtcgcgc
     1561 atgacgcgcg tcggctcggc gagcagtacc cgcaccttgg agaagctgcg cgtcagcacg
     1621 aagtgcttga tcacatcgag aaaggccgtc tgatcgtaaa gatcgggctc gaggtcgggt
     1681 gtgtagatcg agatgagtcg ctgcgccgta gccgcgatct cattgacggc ggcccgtact
     1741 tcagcgagcg aggtgagcac ggtcagcgtt tcggcaccct gccgcgagct ggctgcgccg
     1801 ccggccccgt tggccgactc gggcgcatag cgcgcgtttg caagctgcgc caaagcaccg
     1861 ttgagatcaa gttccccgct tatggccact ggcgtcctgc gtcacctgca cggagggcag
     1921 gttgcggggc atcttagtga cgggcatggg aattcccttg ccgaaccggt cacggtttag
     1981 ggccccgggc tggccacagt aagtcaaaag cgaggcctgg ttcgcacttt gccgggccgc
     2041 cgcagcacca gggcgagcgc gggtggcggc ttcagatctg gcgcgcgagc tgcgcggcga
     2101 ggccgatata gctctccggc cgcaggctcc gcagccgcgc tttgtcgctc gcggcgagcg
     2161 gcagcgcgtc gatgaacgct gccagcgtcg ctgcatcgat ctgccggccg cgcgtgaact
     2221 ccttcagccg ctcatagccg tccggcacac ccgcggcccg taataccgtc tgcaccgcct
     2281 cgccgagcac ctcccagcca gcggcgaggt ccgcgctcat gcgcgccggg tccgcctcga
     2341 tcttctccag ccctcggccg agcgcgcgcc aggcgatgag cgaatgcgca agcgcgaccc
     2401 cgagattgcg cagcacagtg gaatcggtga ggtcgcgctg ccagcgtgac accggcagct
     2461 tctcggcgaa gtgccgcagc agcgcgttgg cgattccgca gtttccctcg gcgttctcga
     2521 agtcgatggg attcaccttg tgcggcatgg tggatgagcc cacctcaccc gccagcgcgc
     2581 gctggcgcag atagccgagc gagacatagc cccacaggtc gcgacacagg ccgatgcaca
     2641 cgacattgac cgctgccacg gcgtcgcagt actcgccgat ccagtcgtgt ggctcgatct
     2701 gagtggtcca ggggttgtag tcgagaccga gcgaggtgac gaaagcctca gcgagcgcgg
     2761 gccaatcgag cgcgggcagg gcggcgatgt gtgcgttgaa gttgcccacg gcgccgttcc
     2821 acttaccgag aatgctcacc gctgcccagc gccgctgcgc gcgcgcgagg cgtgccgcga
     2881 aattggccag ttccttgccg agcgtggtgg gactggccgg ctgcccatgc gtgcgcgcgg
     2941 gcatggcggt gtcggcgtaa cggtgtgcga gctgcgtgag ctcgtgggta cgtgcggcca
     3001 gcaccggcag cagcgcgccg cgcgccgcgc gcagcaggca ggcgtagctg aggttattga
     3061 tgtcttccga ggtacagccg aaatgcacga gctcgagagt tgccgcctgt gccccggccg
     3121 cggcgagccg ctcgcggaca taatactcga ccgccttgac gtcgtgattg atgcgcgcct
     3181 cgatgctttt taccgcggcg gccgcgcccg gatcgggcgc ctgcgccagc tgccgggcgc
     3241 attcgctcac cgccagcgca agcgtggagc ccgcaagttg cggcgctgct gccgccagct
     3301 gcagcagcca ctgcgcctca atacggatgc gctcgcgaac gaggccggct tccgagagca
     3361 gcgcacgcag tggctcggtg gcggcgcggt agcgcccatc gaccggggag agcgcctcga
     3421 gcgccggcag gcaatcgctg gggcttccgg acatgcgcta ctatcataac gcatacggtc
     3481 gttgggccgt gtttccagcc gcggagtgca cgcatgacga ggtccttccg tactgagcgc
     3541 gacagcatgg gcgagctcaa agtaccaacc gaggcgctgt ggggcgcgca gacgcagcgt
     3601 gcgatcgaga actttccgat cagcggcggc agcatgccgc gcgagttcat cgccgcgctg
     3661 gcactggtga agcaggccgc cgcccgcgcc aaccgcggcc tgaaactgct ggatgcgccc
     3721 ctggcgcagg ccatcgacgc ggcggcggcg gaagtggtcg ccggcgcgca caacgcacat
     3781 tttccgctcg atgtgttcca gaccggttcg ggcaccagca ccaacatgaa cgccaacgag
     3841 gtcatcgccg cgctggcgag ccggcacctc ggcaaggcgg tgcaccccaa cgatcacgtg
     3901 aacatgggtc agagcagcaa cgacgcgatc ccgactgcga tccacgtcag tgctgcgctc
     3961 gcgctgcgcc gcgagctgct gccggcgctc gagcacctga gcgatgtgct cagggagaag
     4021 gagcgcgaca ttggcgccat cgtcaagacc ggccgcaccc acctgatgga tgcgatgccg
     4081 ctcacctttg gacaggagct ctccggatgg cgcacgcagg tcgagcacgg cgtggcacgg
     4141 ctgcgcgccg tcgaaccccg gctgctggcg ctggcccagg gcggcaccgc cgtcggcacc
     4201 ggcatcaata cccacgcgga tttcggccgg cgcttctgcc aggagctgtc atcgctcacc
     4261 gcggtgccat tcgtcccggc ggagaactac ttcgaagcgc tgtcggcgca ggacacggca
     4321 gtcgagttgt ccggtcagct caaggtgatc gcggtgagcc tgatcaagat ggccaacgat
     4381 ctgcgctgga tgaacagcgg gccgctcgcc gggctcgggg agatcgcgct gcccgcgctg
     4441 cagccaggca gcagcatcat gcccggtaag gtgaatcccg tgatacccga ggcgacgatg
     4501 atggtgggtg cgcaggtgat cggcaatgac acgaccatca cgctcgccgg ccagtccggc
     4561 aattttcaac tgaacgtcat gctgccggtc attgcccaca acctgctcga gagtattcgc
     4621 ctgctggcga acgtgtcgcg cgcgctggca gacagcgcca ttgcgggctt gcgggtgaac
     4681 gcgacgcgca tcgccgcagc gctcgatcgc aacccgatac tcgtgacggc gctcaatccg
     4741 gtgatcggct acgagaaggg cgcggcgatc gccaagaagg cctacgccga gggccg
//
LOCUS       DATDMP010000035         4155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_671712, whole genome shotgun sequence.
ACCESSION   DATDMP010000035 DATDMP010000000
VERSION     DATDMP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671712"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VI195_00650"
     CDS             complement(<1..656)
                     /locus_tag="VI195_00650"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEY6822920.1"
                     /translation="MSDVIVHTLNVELGTRSYPILIAAGLLGRPQTFAQHLHASELLI
                     VSTTTVAPLYLPMLTASLGKRRSVEVILPDGEAHKTLANAARVLDVLVANRLARDCAV
                     LALGGGVVGDLAGFAAACYQRGVDFVQLPTTLLAQVDSAVGGKTGVNHAGGKNLIGAF
                     HQPVLVVNDTATLATLPPRELRAGLAEVIKYGLIADAALFEWLEDHLEELLAGEPAAL
                     A"
     gene            complement(750..1295)
                     /gene="aroK"
                     /locus_tag="VI195_00655"
     CDS             complement(750..1295)
                     /gene="aroK"
                     /locus_tag="VI195_00655"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEY6822921.1"
                     /translation="MIGKRSVFLVGPMGSGKSAVGRQLARALHLPFHDSDSEIERRTG
                     VDIAFIFEKEGETGFRQREHEALEWLTALEAIVLATGGGAVLLPENRRLLSQRGCVVY
                     LQTSVAQQAERVRHGRTRPLLADGDPATRLGHLMDARAPLYGEIADLVVSTDGRRVNS
                     VAEQVLRELTAARRRGASRPA"
     gene            complement(1372..3537)
                     /locus_tag="VI195_00660"
     CDS             complement(1372..3537)
                     /locus_tag="VI195_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY6822922.1"
                     /translation="MHSSSLMNRLLGAAAAVVLAAGLGLAAPAPARAADQPALQSVDV
                     QPLPGQQVQVTMRLSAPAPQPLSFTIDNPARISFDLPNTTLAMPSKRIDVHAGGVDTI
                     VAAETKDRTRLVLNLDKLVPYDTRVAGNNIVVMLGGGSAASATVAPAAAAPLASGARE
                     LRAIDFRRSADGAGRVMVKLSDPHIHINLHQVGNQVVVDFSDAGVPANLMRRYDATDY
                     GTPVSGFDVTRVGNGSRISISANGDFEQLAYQSDDQYVVEVSPKRRAANAQEDKPVYS
                     GERLTLNFQDIETRAVLQLLADASGQNIVVSDSVSGNVTLRLQNVPWDQALDIVLRTK
                     GLDKRRQDNVIIVAPQAELAAREKAELAARKDVQELAPLRSEYLQVNYAKAKDMEALI
                     KTQSNSLLSPRGSVTVDERTNTLLLQDTSDRLQDIRRLVATLDIPVRQVQIEARIVIV
                     SNDFKRDLGVIFGVTNWQKNGNNGLVYTTGTAAGLDTQQSTFITGQNAINNAINSGTT
                     PVPLPTYGIPAAANRYNVNLPVSNPAGTFALGILGNNFIVDLELSAAQAETQANIISS
                     PRVVTANQKEATIKQGIEIPYQQSASSGATTIQFKDAVLSLKVTPQITPDNRIIMDLD
                     VRDDSVGNVVVASGGVNVPSINTREIATQVLVNDGQTVVLGGILETTQREDDNKVPYL
                     GDIPVLGHLFKQTSHQDNKDELMIFITPKIVHEGVNVYN"
     gene            complement(3560..>4155)
                     /locus_tag="VI195_00665"
     CDS             complement(3560..>4155)
                     /locus_tag="VI195_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY6822923.1"
                     /translation="PPAEAGGGGLTTMTLRSRGPGMNDHTYRCGWRLLSVALACCYAA
                     LTACSSADDELARFIADTKKEPGGRVEPLPEIKPYETFVYAAQDLRSPFLPSSPGAGA
                     GLAGVRPDQKRSREFLEQYSLDTLKMVGTLRLGGQMYGLVETKDGLVHRVTTGNHLGQ
                     AEGKITDISPSKINLIEIVPDSLGGYMERPAALGLNE"
BASE COUNT          648 a         1343 c         1404 g          760 t
ORIGIN      
        1 gcgagcgccg cgggctcacc ggccagcagc tcttcgaggt gatcttccag ccactcgaac
       61 agcgcggcgt cggcgatcag tccgtacttg atcacctccg cgagccccgc gcgtaactcg
      121 cgcggcggga gcgtggcaag cgtcgcggtg tcgttgacca cgagcaccgg ctggtggaat
      181 gcgccaatca ggttcttgcc accggcatgg ttcacgccgg tcttgccgcc caccgccgag
      241 tccacctgcg ccagtagcgt agtgggcagc tgcacgaagt cgaccccgcg ctggtaacag
      301 gccgcggcaa agcccgccag gtcgccgact accccgccgc cgagcgcgag cacggcgcag
      361 tcgcgcgcca ggcggttggc gaccagcacg tccagcaccc gcgccgcgtt ggcgagtgtc
      421 ttgtgggcct caccatccgg caggatcacc tcgacgctgc ggcgcttgcc gaggctggca
      481 gtgagcatcg gcagatagag cggcgcgacg gtggtggtgc tgacgatgag cagctcactg
      541 gcatgcaggt gctgcgcgaa tgtctgcgga cgaccgagca atccggcggc gatcaggatg
      601 ggataactgc gagtcccgag ttcgacgtta agggtgtgca cgatcacgtc gctcacgttg
      661 cggctggcaa caggggcgcc cgcagtatac gtgagcgccc gccggcaacg gtggcccctg
      721 gcgcggcctc gccaaacttc ggctcctgat caagccggcc gcgacgctcc acgccggcgt
      781 gcggcagtca gctcgcgcag cacttgctcg gctacgctgt tgacccggcg gccatcggtc
      841 gagactacga ggtcggcgat ctcaccgtag agcggcgcgc gcgcatccat gaggtgcccg
      901 aggcgcgtgg caggatcgcc gtccgcgagc aatgggcggg tgcggccatg gcgtacgcgc
      961 tcggcctgct gtgccacgga ggtctgcaga tacaccacgc agccgcgctg actcagcagg
     1021 cgccggttct ccggcagcag taccgcgccg cccccggtgg cgagcacgat ggcctcgagc
     1081 gcggtgagcc actcgagcgc ttcgtgctcg cgctggcgaa agcccgtctc gccttctttc
     1141 tcgaagatga aggcgatgtc gacgccggtg cgccgctcga tctcgctgtc actgtcgtgg
     1201 aagggcaggt gcagtgcccg cgcaagctgg cggccaaccg cactcttgcc cgagcccatc
     1261 gggccgacga ggaatacgct tctctttccg atcattgcgc tcgacaggat acccaaaaaa
     1321 agtgcggccg cccgaaggcg gccgcgctca actccctgat gacccagtcg ttcagttata
     1381 tacgttcacg ccctcgtgca cgatcttcgg cgtgatgaag atcatcagct cgtccttatt
     1441 gtcctggtgg ctggtctgct tgaacaggtg cccaaggacc ggaatatcac ccagatacgg
     1501 caccttgttg tcgtcctcgc gctgtgtcgt ctcgaggata ccgccaagca cgaccgtctg
     1561 tccgtcgttc accagcacct gcgtggcgat ctcgcgcgtg ttgatcgagg gcacgttcac
     1621 gccgccggag gccacaacca cgttgccgac gctgtcatcg cgcacgtcca ggtccatgat
     1681 aatgcgattg tcgggggtga tctgcggggt caccttgagc gacagcaccg cgtccttgaa
     1741 ctgaatggtc gtggcgccgc tcgaggcaga ctgctggtac gggatctcga taccctgctt
     1801 gatggtcgcc tccttctggt tggcggtcac cacgcgcggt gaggagatga tgttcgcctg
     1861 ggtttccgcc tgcgcggcgc tcagctcaag atcgacgatg aagttattgc cgaggatgcc
     1921 gagcgcaaac gtgcccgccg ggttgctcac cggcaggttg acgttgtagc ggttcgccgc
     1981 ggccggaata ccgtaggtcg gcagcggcac cggcgtcgtg ccggagttga tcgcgttgtt
     2041 gatcgcgttc tgtccggtga tgaaggtcga ctgctgcgta tcgaggccag ccgcggtgcc
     2101 ggtggtatag accaggccgt tattgccgtt cttctgccag ttggttacac cgaatatcac
     2161 accgaggtcg cgcttgaagt cgttgctgac gatgacgatg cgcgcctcga tctgcacctg
     2221 gcgtaccggg atgtcgagcg tcgccaccag gcggcggatg tcctgcagtc ggtccgaggt
     2281 gtcctgcagc agcagggtat tggtgcgctc atccaccgta acgctgccgc gcggcgagag
     2341 cagtgagttg ctctgcgtct tgatgagcgc ctccatgtcc tttgccttgg cgtagttcac
     2401 ctgcaggtac tcggagcgca gcggcgcgag ttcctgcacg tccttgcgcg cggcgagctc
     2461 ggccttctcg cgcgcagcga gctcggcctg cggagcaacg atgatgacgt tgtcctggcg
     2521 gcgcttgtcg agtcccttgg tacgcagcac gatgtcgagc gcctgatccc acggcacgtt
     2581 ctgcaggcgc agcgtgacgt tgccgctcac cgagtcgctc accacgatgt tctggccgct
     2641 ggcgtcggcg agcagctgca gcaccgcgcg cgtctcgatg tcctggaagt tgagggtgag
     2701 ccgctcgccg ctgtacaccg gcttgtcctc ctgcgcattg gcggcgcggc gcttgggaga
     2761 gacctccacc acgtactggt cgtcggactg ataggcgagc tgctcgaaat cgccattggc
     2821 ggagatcgag atgcgcgagc cgttgccgac gcgggtgacg tcgaagccac ttaccggggt
     2881 gccgtagtcg gtagcgtcgt agcggcgcat gaggttggcc ggcacgcccg catcgctgaa
     2941 gtcgaccacc acctggttgc cgacctggtg caggttgatg tgaatatggg ggtccgaaag
     3001 cttcaccatc actcgtcccg cgccatcggc gctgcgacga aagtcgatgg cacgcagctc
     3061 acgcgcgcca ctggcgagcg gggcggcggc cgccggagcc accgtggcgc tcgcggcact
     3121 gccgccgccg agcatcacca cgatgttgtt gccggccacg cgcgtgtcgt agggcacgag
     3181 cttgtcgagg ttcagcacca ggcgcgtacg atccttggtc tcggccgcga cgatggtgtc
     3241 gaccccgccg gcgtgtacat cgatgcgctt cgatggcatc gcgagggtgg tgttcggcag
     3301 gtcgaaggag atgcgcgccg ggttgtcgat ggtgaacgac agcggctgtg gggcgggtgc
     3361 cgacaggcgc atggtgacct gcacctgctg tcccggcagg ggttgcacgt caaccgactg
     3421 cagcgcgggt tgatccgccg cgcgggccgg agctggcgct gccaggccga gtcccgcagc
     3481 gagcaccacc gccgcggcgg cgccgaggag acgattcatc aggctggacg agtgcattct
     3541 gagactccct gctggacctt tattcgttca ggccgagcgc agcggggcgc tccatgtacc
     3601 cgccgaggct gtccggaacg atttcaatga gattgatttt cgatgggctg atgtcggtga
     3661 tcttgccttc cgcctgcccc aggtggttgc cggtggtgac ccgatgcacg agtccatcct
     3721 tggtctccac cagcccgtac atctgtccgc cgagacgcag cgtgccgacc attttcaacg
     3781 tgtcgagcga gtactgttcg aggaactcgc ggctgcgctt ctgatcggga cgcacaccgg
     3841 caagtccggc tcccgcgccg gggctgctcg gcaggaaggg cgagcgcaga tcctgggccg
     3901 cgtacacgaa ggtctcgtag ggcttgatct ccggcagcgg ctcgacgcga ccgccgggct
     3961 ctttcttcgt atccgcgatg aaacgcgcga gctcgtcatc ggcactcgag caggccgtga
     4021 gcgcagcgta acagcaggcg agcgcgacgc tcagcagccg ccatccgcat cggtaggtgt
     4081 ggtcattcat gcccgggccc cgcgatcgca gcgtcattgt tgtcaacccg cccccgccgg
     4141 cttcggccgg tgggt
//
LOCUS       DATDMP010000036         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_894471, whole genome shotgun sequence.
ACCESSION   DATDMP010000036 DATDMP010000000
VERSION     DATDMP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894471"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..>2314)
                     /locus_tag="VI195_00670"
     CDS             complement(135..>2314)
                     /locus_tag="VI195_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6822924.1"
                     /translation="AAMILLFLGDWRPTIIIAISIPLSICVSIILLSALGETINIMTL
                     GGLALAVGILVDDATVTIENINMHLEQGQQVEEAILNGAHEIALPALVSTLAICIVFV
                     PMFMLTGIARYLFVPLAEAVVFAMLASYLLSRTLVPTLAKYWLQLHEGHTSPPRHAAQ
                     RLQRRFEEWFQRTRLGYRALLERALAAGPRFALPFLGAMAATALLAFPLGPFLPGLGQ
                     DFFPSVDAGQIKLHLRAPTGIRIERTAELCDSIEATIRSVIPAHEISTIVDNIGLPYS
                     GINLSYSTSAPVGPGDADILINLAPDHHATERYVHALREKVNALYPGTSFAFSPADIV
                     SQILNFGLPAPVDVQVSGFNTTETRKYANAILTRMRTIPGLVDARIQQQYDYPTLNVD
                     VDRTKASLLGLTETQVASDLLVSLSGSSQTTLSFWIDPNTGTQYPVVAQTPQFRLTSL
                     TDLATTPVTAGGATEANNANDGSAATAPSSAAGGGVQLLANLATFRRSESPAVVSHYN
                     STPVIDIYAGLERTDLGFIAKQIERIVHDTQSLRPRGAQVTVRGQVTTMTESFTGLLI
                     GLAGAVVLVYLLIVVNFQSWRDPFIIITALPAALAGIVWMLFLTHTPVSVPALTGAIM
                     CMGVATANSVLVISFARQRMDAGDDAAHAALQAGFTRFRPVLMTALAMIIGMLPMALG
                     LGEGGEQNAPLGRAVIGGLVFATIATLFFVPTVFSIIHGRRSA"
BASE COUNT          426 a          692 c          835 g          361 t
ORIGIN      
        1 attgcgcgtc atcaggcggc tgaggattcc ccacaccgcg agcagcagca cgagcgcgat
       61 cgctacccac aggtaacgcc gacgggaggg agtcaacgcg gtcgccggcc gagggcttgc
      121 cgagacatca ttcatcaggc actccggcgt ccgtggatga tgctgaatac ggtaggaacg
      181 aagaacaagg tggcgatggt ggcgaacacc agaccgccga tcacggcgcg cccgaggggc
      241 gcgttctgct cgccgccttc accgaggccg agcgccatgg gcagcatgcc gatgatcatg
      301 gcgagcgcag tcatcaacac cggccggaag cgggtgaacc ccgcctgcag cgccgcgtgg
      361 gcggcgtcat cgccggcgtc catgcgctgg cgcgcgaagc tgatgaccag cacgctgttg
      421 gcggtggcga cgcccatgca catgattgcg ccggtgagcg cgggcacgct cacgggtgtg
      481 tgggtgagga acagcatcca gacgatgccg gcaagcgccg ccggcagtgc ggtgatgatg
      541 atgaacggat cgcgccagga ttggaaattg accacgatca acaagtagac gagcaccacc
      601 gcacccgcaa gaccgatgag cagcccggtg aaggactcgg tcatggtggt cacctggccg
      661 cgaaccgtga cctgcgcacc gcgcggtcgc agagactgcg tgtcgtgcac gatccgctcg
      721 atctgcttgg cgatgaaacc gagatcggtg cgctcgaggc ccgcatagat atcgatgacc
      781 ggggtggagt tgtagtggct cacgaccgcc ggggactcgc tgcgccggaa agtcgcgagg
      841 ttggcgagca gctgcacgcc accgcccgcg gcactgctcg gcgccgtggc ggcgctgccg
      901 tcattggcat tgttcgcctc cgtggcaccg cctgcggtga ccggagtcgt cgccagatcg
      961 gtgagcgagg tgaggcggaa ctgcggtgtc tgggcaacca ccgggtactg ggtgccggtg
     1021 ttcggatcga tccagaagga cagcgtggtc tgcgagctgc ctgagagcga cacgagcaga
     1081 tccgaggcca cttgcgtctc ggtgagcccg aggagcgagg ctttggtacg gtcgacgtcc
     1141 acattcagcg tcgggtagtc gtactgctgc tggatgcgcg cgtccaccag tcccggaatc
     1201 gtgcgcatgc gcgtgaggat ggcattggca tacttgcgcg tctcggtggt gttgaagccc
     1261 gacacctgca catcgaccgg ggcgggcagg ccaaagttga ggatctggct gacgatgtcg
     1321 gccggcgaga aggcgaagct ggtgccggga taaagcgcgt tcaccttctc gcgcagcgcg
     1381 tgcacgtagc gctcggtggc atgatggtcg ggggcgagat tgatcagaat atcggcgtcg
     1441 ccggggccaa ccggtgccga ggtcgaatag gagaggttga tgccgctgta gggcaggccg
     1501 atgttatcga cgatggtcga gatctcgtgc gccgggatta cgctgcggat ggtcgcctcg
     1561 atgctgtcgc agagctccgc ggtcctctcg atgcgtatgc cggtgggcgc tcgcaagtgc
     1621 agcttgatct gtcccgcatc gacactcggg aagaagtcct ggccgagccc cggcaggaag
     1681 ggaccgagcg gaaaggcgag cagcgcggtc gctgccattg cgccgaggaa gggcagcgcg
     1741 aagcgcggac ccgcggcgag cgcccgctcg agcagtgccc ggtagcccaa ccgggtgcgc
     1801 tggaaccact cctcgaagcg ccgctgcagg cgctgggccg catgccgtgg tgggctcgtg
     1861 tgcccctcgt gcagctgcaa ccagtacttg gccagcgtcg gcacgagcgt acgcgacagc
     1921 aggtaggagg ccagcatcgc gaacaccacc gcctcggcga gcggcacgaa caggtatcgg
     1981 gcgatgcccg tcagcatgaa catcggcacg aacacgatgc agatggcgag cgtcgacacc
     2041 agggcgggga gggcgatttc gtgggcgccg ttgaggatcg cctcctcgac ctgctggccc
     2101 tgctcgagat gcatgttgat gttctcgatg gtcacggtcg cgtcatcgac cagaatgccg
     2161 accgcgagcg cgagcccgcc gagcgtcatg atgttgatgg tctcgcccag cgcgctcagc
     2221 aggatgatcg aaacgcagat cgagagcggg atcgagatgg cgatgatgat cgtcggccgc
     2281 cagtcgccga gaaagagcag gatcatggcc gcgg
//
LOCUS       DATDMP010000037         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1671975, whole genome shotgun sequence.
ACCESSION   DATDMP010000037 DATDMP010000000
VERSION     DATDMP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671975"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="dusA"
                     /locus_tag="VI195_00675"
     CDS             complement(<1..557)
                     /gene="dusA"
                     /locus_tag="VI195_00675"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY6822925.1"
                     /translation="MTVPPRHGSAHRLCVAPMMDWTDRHCRYFLRQVSSSARLYTEMI
                     TTGALIHGDVDRHLAFNEEEHPVALQLGGSEPDELAQCAKLGERYGYDEINLNIGCPS
                     ERVQRGAFGACLMAEPTLVSDCVKAIKDKTSLPVTVKHRIGIDAVEDYGFVHAFVAEV
                     SKAGCDTFIVHARNAVLKGLSPKENR"
     gene            560..994
                     /locus_tag="VI195_00680"
     CDS             560..994
                     /locus_tag="VI195_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY6822926.1"
                     /translation="MASLPRQQIGTRLKEFEQKEAERILSACTQCGKCFEVCPMTKYS
                     KAPATDGKKVVPGVLAVLRGEPGSPEALGWIAVCTRSGSCVPACPEHVDPRMMMRLAR
                     MTALGGRELPAQIPAKEDPDYFNRIRAFAQLQLSDDEIKKWT"
     gene            1016..1855
                     /locus_tag="VI195_00685"
     CDS             1016..1855
                     /locus_tag="VI195_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY6822927.1"
                     /translation="MAERITFWYGCNVLRHGDIIHACLDILRALGFDAAPAGGPDYCC
                     GTVKDANQTTAGGMATRTVGRFNAMERDRVVAWCPSCHSHMHDFMERANDRNFGMTYF
                     VELLHQHREQLKPLLKTPVPMRVAIHKHVGFQDLVPVNRLVPDLLRLIPGIEVVEHEH
                     VSPGYMCASFAGMPQALKDINAAMVAAVKETRADAAVTVFHQCYRELCGLDAAGITPV
                     YNYVHLFARSMGLDYEDEYKAWKKTPDAAAGMIGAERLAKVGVQFYERAILPELRKRP
                     DVS"
     gene            complement(1857..>2325)
                     /locus_tag="VI195_00690"
     CDS             complement(1857..>2325)
                     /locus_tag="VI195_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY6822928.1"
                     /translation="LAVMMGTLIVGLASVIEAMGWLFDWLRIAGAVYLVWLGWKLLRS
                     PDMAIDVKRLSKPRGGFFLQGLMVLLANPKALLWFGAFIPQFIDARGNYVAQIVVLGL
                     TAMATATLTDGAYAVLTGRAGVMLTRHRVRIVSRVSGACLIGGGVWLALTRSR"
BASE COUNT          415 a          766 c          778 g          366 t
ORIGIN      
        1 cggttttcct tgggcgagag gcccttcagc acggcattgc gcgcatggac gatgaacgtg
       61 tcgcagccgg ccttcgagac ctcagcgacg aatgcgtgca cgaagccgta gtcctcgacc
      121 gcgtcgatgc cgatccggtg cttcacggtc acgggcaagg acgtcttgtc cttgatggct
      181 tttacgcaat cggagaccag agtcggctcc gccatcaggc aggcgccgaa cgcgccccgc
      241 tgcacgcgct cgctcgggca gccgatgttg aggttgatct catcgtagcc gtagcgctcg
      301 ccgagcttcg cgcattgggc gagctcgtcc ggctcgctgc cgccgagctg cagcgccacg
      361 ggatgctctt cctcgttgaa ggcgagatgc cgatccacgt cgccgtggat gagcgccccg
      421 gtggtgatca tctctgtgta gagccgggcg ctcgaagaca cctggcgcag gaagtagcgg
      481 cagtgccggt cggtccagtc catcatcggc gccacgcaca ggcggtgggc gctgccatgt
      541 cgaggtggta cagtcactca tggccagtct accccgccag cagatcggca cgcggctgaa
      601 ggagttcgag cagaaggaag ccgagcgcat cctctccgcc tgcacgcagt gcggcaagtg
      661 cttcgaagtg tgcccgatga cgaaatactc gaaggcgccg gccaccgacg gcaagaaagt
      721 cgtgcccggc gtgcttgcgg tgctgcgcgg cgagccgggc agccccgagg cgctcggctg
      781 gatcgcggtg tgcacgcgca gcggctcgtg cgtcccggcg tgccccgaac acgtggatcc
      841 ccgcatgatg atgcggctcg cgcgcatgac tgccctgggc ggccgggaac tgcccgccca
      901 gatccccgcg aaggaggatc cggactactt caaccgcatc cgtgctttcg cgcagctgca
      961 gctgagcgac gacgagatca agaagtggac gtgaacgcaa aaggtgtcgc tgaacatggc
     1021 tgagcgcatc accttctggt acggctgcaa tgtgctgcgc catggggaca tcatccatgc
     1081 ctgcctggac atcctgcgcg cgctcggctt cgacgcggcc ccggccgggg gccccgatta
     1141 ctgctgcggc acggtcaagg atgcgaacca gacgaccgcc ggcggcatgg cgacgcgcac
     1201 ggtcgggcgg ttcaacgcga tggagcgcga ccgggtcgtc gcctggtgcc cctcgtgcca
     1261 ctcgcacatg cacgacttca tggagcgcgc gaacgaccgc aatttcggca tgacctattt
     1321 cgtggagctc ctgcaccagc accgggagca attgaagccc ttgctgaaaa cgcccgtgcc
     1381 gatgcgcgtc gcgatccata agcacgtggg tttccaggac ctcgtgccgg tgaaccggct
     1441 ggttcccgat ctgctgcggc tcattccagg aatcgaggtg gtggagcacg agcatgtttc
     1501 tccgggctac atgtgcgcct cgtttgccgg catgccgcag gcgctgaagg acatcaacgc
     1561 cgcgatggtg gccgcggtga aggaaacgcg cgcggatgcc gcggtcaccg tctttcacca
     1621 gtgctaccgc gagctgtgcg gcctcgacgc ggccggcatc acgccggtct acaactacgt
     1681 gcacctcttc gcacgctcaa tgggcctcga ctacgaagat gaatacaagg cctggaagaa
     1741 gacgcccgac gcggcggccg gcatgatcgg cgccgagcgc ctcgcgaagg taggcgtgca
     1801 gttctacgag cgcgccatcc tgcccgagct gcgcaagcgc ccggacgttt cctagctcaa
     1861 cgcgagcgcg taagcgcgag ccacacgccg ccgccgatga ggcaggcgcc gctgacgcgg
     1921 gagacgatgc gtacgcggtg ccgggtcagc atcacgccgg cccgcccggt aaggaccgca
     1981 taggcgccgt cggtgagcgt cgccgtcgcc atggccgtca gcccgaggac gacgatctgc
     2041 gcgacgtaat tgccgcgagc atcgatgaac tgcgggatga aggcgccgaa ccagagcagc
     2101 gccttcggat tggcgagcaa gaccatcagg ccctggagga agaagccgcc gcggggcttg
     2161 gacaggcgct tcacgtcgat cgccatgtcc gggctgcgca ggagcttcca gcccagccac
     2221 acgaggtaca cggcgccggc gatcctgagc cagtcgaaga gccagcccat cgcttcgatc
     2281 accgaagcca gccccacgat gagcgtgccc atcatgacgg cgagg
//
LOCUS       DATDMP010000038         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_672139, whole genome shotgun sequence.
ACCESSION   DATDMP010000038 DATDMP010000000
VERSION     DATDMP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672139"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VI195_00695"
     CDS             <1..974
                     /locus_tag="VI195_00695"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEY6822929.1"
                     /translation="SVSGHGADSTFLNGDARYQLWRDTGVGAVANVLLARDGMHHTAW
                     SVEGYLDNTNSHGSGRAQLDYADDSQTQDAILTLQQSWNMSTGARLSTSAGVEHIRTS
                     AQAALPAGDSTILRLAAYGGGNLTARFSLDGSVQWAQAVQGRAAPYTSADVSLNYQIN
                     RCWAVIFTYYENRIGSWTPLVVTSPLTPAVPVPTEAEGQRGIFLTLRFQDARGGHFVP
                     LGGAPGAGSGRLTGVVYLDANENGRFDAGEAGAPNVTVILDGRFSTRTDANGRFDFPA
                     VVAGHHVLTVQTDNIPLPWALVNGGRTEVEVLTRDRVDVNIGALRLK"
     gene            complement(1017..1406)
                     /locus_tag="VI195_00700"
     CDS             complement(1017..1406)
                     /locus_tag="VI195_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY6822930.1"
                     /translation="MAYVTVINEHQHVQQIVSGHHHLTADEPVSAGGSDAGFAPRELL
                     LASLGADTAMALRRHAMQNDWTLGKITVGVRWSRDERGREHIERRLSFARALTGEQKA
                     RLLEVASATAVTSVLKPGIAIQSTVID"
     gene            complement(1381..2418)
                     /locus_tag="VI195_00705"
     CDS             complement(1381..2418)
                     /locus_tag="VI195_00705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6822931.1"
                     /translation="MRSCDVLIVGGGPAGSTAAWQLQRAGADVLLLDRERFPRVKLCA
                     GWITPEVVRDLAIDLASYPHRLLTFPRLRVHLGRLHVPVPCVQHSIRRFEFDAWLLER
                     SGVEVVQHNVRHIRREADAFIVDDEFRCRYLIGAGGTRCPVYRELFRELNPRASALQI
                     VTLEHELPYDWSDGDCHLWFFEQGLPGYSWYVPKERGWLNVGVGAIAERLKARGQDIR
                     THWAHLAATLERRLAPRAQYEPTGYSYYLLGKVEVVRRENAFITGDAAGLASRDLGEG
                     IGPAIRSGLRAARAILANEPYHLDDVTGLSIGSIARSMLGRLKPAGADYGRRTRAATG
                     GSAHGLRHGDQ"
     gene            complement(2427..>2646)
                     /locus_tag="VI195_00710"
     CDS             complement(2427..>2646)
                     /locus_tag="VI195_00710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6822932.1"
                     /translation="YARTLEHWLQRFEGSRQPVTAMFDERFVRMWRLYLAGSVAAFST
                     GSLQLFQVVFAPGESNDIPWTRAHVYTR"
BASE COUNT          444 a          937 c          869 g          396 t
ORIGIN      
        1 tctcggtgag cggccatggc gccgacagca ccttcctcaa cggcgatgcg cgctaccagc
       61 tgtggcgcga caccggcgtc ggcgcagtag ccaacgtgct gctggcgaga gatggcatgc
      121 accacaccgc ctggtcggtc gaaggctatc tcgacaatac caactcgcac ggcagcggcc
      181 gcgcgcagct cgactatgcg gacgactcgc agacgcagga tgcgatcctc accctgcagc
      241 agagctggaa catgagcacc ggcgcccggc tctcgaccag cgccggggtc gagcacatcc
      301 gcaccagcgc gcaagccgcg ctccccgcgg gcgacagcac catcctgcgt ctcgcggcct
      361 acggcggcgg taacctcacc gctcgcttct cgctcgatgg cagcgtgcag tgggcgcagg
      421 ccgttcaggg gcgcgccgcc ccgtacacct cggcggatgt ctcgctcaac taccagatca
      481 accgctgctg ggcggtcatc ttcacctatt acgagaaccg aatcggctcg tggaccccgc
      541 tcgtcgtgac ctctccgctc acccctgcgg taccggtacc gaccgaggcc gaggggcagc
      601 gcggcatctt cctcacgctg cgcttccagg atgcgcgtgg cggccatttc gtgccgctcg
      661 gcggcgcccc gggcgcaggc tcgggccgcc tcaccggggt ggtgtatctc gatgccaacg
      721 agaatggccg cttcgatgct ggtgaagcgg gcgcccccaa tgtcaccgtc atcctcgatg
      781 gccgcttctc cacacgcacc gatgccaacg gccgcttcga tttccccgcg gtggtggccg
      841 ggcaccatgt gctcaccgtg cagaccgaca acattccgct gccgtgggcg ctcgtcaatg
      901 gcggccgcac cgaggtcgaa gtgctgacgc gcgaccgggt cgatgtgaat atcggcgcgc
      961 tgcgcctgaa gtgaccgcgc ctgaagtaac ggccgaaagt gaccgatcgg ccgcgctcag
     1021 tcgatgaccg tcgactggat cgcaatgccc ggcttcagga ccgaggtcac cgcagtggcg
     1081 gaggccacct cgagaagccg cgctttctgt tcccccgtca acgcacgcgc gaaggacagc
     1141 cgccgctcga tatgctcgcg cccgcgctca tcgcgcgacc agcgcacgcc gaccgtgatc
     1201 ttccccaggg tccagtcgtt ctgcatcgca tgcctgcgca gcgccatcgc ggtgtcggcg
     1261 ccgaggctcg cgagcagcag ctcgcgcggt gcaaagccgg catcgctacc gccggccgag
     1321 acgggctcgt cagcggtcag gtgatgatgt ccggagacga tctgctgtac gtgctggtgc
     1381 tcattgatca ccgtgacgta ggccatgtgc acttcctccc gtcgccgcac gcgtccgccg
     1441 cccataatcg gcacctgcgg gcttgagccg cccgagcata ctgcgggcga tgctgccgat
     1501 gctcaacccg gtgacatcat cgagatggta cggctcgttg gcgaggatgg cacgcgcggc
     1561 gcgcagcccg ctgcggatgg caggaccgat accctccccg agatcccgcg aggcaagtcc
     1621 cgccgcatcc ccggtgatga aggcattctc gcgccgcacg acctccacct tgccgagcag
     1681 gtagtagctg tagccggtcg gctcgtactg cgcacgcggt gccaggcgcc gctcgagagt
     1741 cgcggcgagg tgcgcccagt gcgtgcggat atcctggccg cgcgctttca ggcgctcggc
     1801 gatggcgccc accccgacat tcaaccagcc gcgctccttc ggcacgtacc acgaataccc
     1861 tggcagtccc tgctcgaaga accacaggtg gcagtcgcca tccgaccagt cgtatggcag
     1921 ctcgtgctcg agggtgacga tctgcagcgc gctcgcgcgc gggttcagct cgcggaagag
     1981 ctcgcggtac accgggcagc gcgtgccgcc ggcgccgatc aggtagcggc agcggaactc
     2041 atcatcgacg atgaacgcgt ccgcctccct gcggatgtgg cgcacgttgt gctgcacgac
     2101 ctccacgccc gagcgctcga gcagccaggc gtcgaactcg aagcgccgga tcgagtgctg
     2161 cacgcagggc accggcacgt gcaggcggcc gagatgtacc cgcagccgcg ggaaggtgag
     2221 cagccggtgc ggatagctcg ccagatcgat ggcgaggtcg cgtaccacct cgggtgtgat
     2281 ccagccggca cacagcttca ctcgcgggaa gcgctcgcga tcgagcagta gcacgtcggc
     2341 gcccgcgcgc tgcagctgcc aggcggcggt cgagccggcg gggccgccgc cgacgatgag
     2401 cacatcacag ctgcgcatga acgaggtcag cgcgtgtaga cgtgcgcgcg cgtccagggg
     2461 atgtcgttgc tctcgcccgg ggcaaagacg acctggaaga gctgcagcga gcccgtggag
     2521 aaagccgcca ccgagccggc gagatacagc cgccacatgc gcacaaagcg ctcatcaaac
     2581 atggccgtca ccggctggcg cgaaccctcg aagcgttgca gccagtgctc caacgtgcgg
     2641 gcatag
//
LOCUS       DATDMP010000039         3162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_339620, whole genome shotgun sequence.
ACCESSION   DATDMP010000039 DATDMP010000000
VERSION     DATDMP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339620"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1630)
                     /locus_tag="VI195_00715"
     CDS             complement(2..1630)
                     /locus_tag="VI195_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822933.1"
                     /translation="MFIYKKPPRSHALGEPLRHENHPRPRTRREFIAAGFLSGPAMVM
                     APAWLGALLKANRANAGAPPLDNDIAPLLAAAECNVPASGVGLPFIAFDLAGGANLVG
                     SEVIVGQQGGQSNFLTTAGYGKLGVPGNMVPSASANVDASLGLLWHADGAIKRGIMSM
                     ITPATAAAVNGAVVCAMSQNDTQTNPHNPMYGIAMAGRKGQLLQLIGTESSVSGGNSM
                     APMELIVPALQPSTISQPSDATSLVPIPPGGKQDPLSVEVLETQTRISTGTGLYNGGS
                     GGTAAFTGALGMPTALYANAQQDATLKNQVRCAYVKAVNTANQFGNPSALDPTQDSSI
                     TAIGAKFQANAFSDNEFAKTATVMKLVLNGFAGAGTITMGGYDYHDGSRATGETRNFK
                     AGQMIGAVLQYASALGVPVMIYVFSDGSLNSNSMVDNSAAGRGKLGWQGDNSSVASTF
                     FLVYNPKGRPALRGAASTQQIGYFTADGSVVSTSSPAANSVDSLVQLVLLNYMGLSGT
                     DANFPTAFQNWTGMPQGLGSASQLAAMTVFQPIV"
     gene            complement(1659..>3162)
                     /locus_tag="VI195_00720"
     CDS             complement(1659..>3162)
                     /locus_tag="VI195_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY6822934.1"
                     /translation="LSLSGNVSWVGGWGIHIGAGGKAQASTRASAKFAGMIQPAGEYT
                     IETWVAPANVTQTNAWIVSYSGSNTTRNMTLGQDAMQYQGFARSSTTSTAGTPPLITT
                     TNGGAAQAALQHVVLTYDPIAGQKLYVNGVYTGDADPSKGGSLANWDSTFALVLGNET
                     TGQRQFQGVIKFVAIHNRALTAAQIQQNFAAGVGQKFFLLFGVSQLTGVNQSYILFQG
                     SQYDSYSYLFAQPKFISLDANAMPANLPISGIRIGVNGVLAPAGQAFTSVASSVGGSN
                     YTAANGQLLSPLGTVVPATLGPANDLFFLSFDQIGTHVHAYVDPTPPGTPPTPPSAPQ
                     PDFGVATFERVNHSLARITGVAVTDSVVNPLYQASQQSMPAGPLIAAFGSAQQTAISQ
                     LASAYCGEMMASSTLRDAFFGGNSLDASLNSSAAGFFGASGSAPRQTVINALINKAVG
                     NANPQTASAVQTEVDALITKIPSLNPSATVAQATVAACTAVLGSAAVTLQ"
BASE COUNT          483 a         1002 c         1128 g          549 t
ORIGIN      
        1 atcacacgat cggctggaac accgtcatgg cggccaactg gctggccgat cccaggccct
       61 ggggcatgcc agtccagttc tggaacgcgg tggggaaatt cgcgtcggtg ccgctgagtc
      121 ccatgtagtt gagcaacacc agctgcacca gggaatcgac cgagttggcg gccgggctcg
      181 aggtgctcac caccgagccg tccgcggtga aatagccgat ctgctgcgtc gaggcggcgc
      241 cgcgcaacgc cgggcgcccc ttggggttgt agaccaggaa gaaggtcgag gccaccgagg
      301 agttgtcgcc ctgccagccg agcttgccgc ggccggcggc gctgttgtcg accatactgt
      361 tggaattcag cgagccgtcg ctgaagacgt agatcatcac cggcacgccg agcgcactcg
      421 cgtactgcag caccgcgccg atcatctggc cggccttgaa gttgcgcgtc tcgccggtgg
      481 cgcgcgagcc gtcgtgatag tcgtagccgc ccatggtgat ggtaccggcg ccggcgaagc
      541 cattcagcac cagcttcatc accgtcgccg tcttggcgaa ctcgttgtcg gagaaggcgt
      601 tcgcctggaa tttggcgccg atggcggtga tgctgctgtc ctgggtcgga tcgagcgccg
      661 aggggttacc gaactggttg gcggtgttga ccgccttcac ataggcgcag cgcacctgat
      721 tcttgagggt agcgtcctgc tgcgcattgg catacagcgc cgtcggcatg ccgagcgcgc
      781 cggtgaaggc cgccgtgccg ccgctgccgc cgttgtacag cccggtgcca gtgctgatgc
      841 gggtctgcgt ctcgagcacc tccaccgaca gcgggtcctg cttgccgccc gggggtatcg
      901 gcaccaggga agttgcatcg gagggctgac tgatggtgct cggctgcagc gccggaacga
      961 tgagctccat gggcgccatc gagttgccgc ccgagacgct cgactcggtg ccgatcagct
     1021 gcagcagctg gcccttgcgg cccgccatgg cgatgccgta catcggattg tgcggattcg
     1081 tctgggtgtc gttctgcgac atggcgcaga ccaccgcgcc gttgaccgcg gcggccgtgg
     1141 cgggggtgat catgctcatg atgccgcgct tgatggcgcc atccgcatgc cacagcagac
     1201 cgagcgaggc gtcgacgttg gcgctcgcgc tcggcaccat gttgcctgga acgccgagct
     1261 tgccgtaacc ggcggtggtg aggaagttgc tctggccgcc ctgctggccg acgatcacct
     1321 ccgagcccac caggttcgcc ccgcccgcaa ggtcgaaggc gatgaagggc aaacccacac
     1381 ccgaggccgg cacgttgcac tcggccgccg cgagcagcgg agcgatgtcg ttgtcgagcg
     1441 gcggtgcgcc ggcgttggca cggttcgcct tgagcagcgc gccgagccag gcgggcgcca
     1501 tcaccatcgc cggtcccgag aggaagcctg ccgcgatgaa ctcacgccgc gtcctggggc
     1561 gcgggtgatt ctcgtgccgc agcggctcac cgagcgcatg cgatcgcggg ggctttttgt
     1621 agatgaacat cggcttatct ccaaaaccct gttgagagct actgcaaggt cacggccgca
     1681 ctgccgagca ctgcggtgca ggccgccacg gtcgcctgcg caacggtggc gctggggttg
     1741 agcgagggga tcttggtgat gagcgcatcc acctcggtct gcaccgcgct cgcggtctgc
     1801 gggttggcgt tgccgacagc cttgttgatg agcgcgttga tgacggtctg gcgaggcgcg
     1861 ctgccgctcg cgccgaagaa gccggcagcc gagctgttga ggctcgcatc caggctgttg
     1921 ccgccgaaga aggcgtcgcg caacgtgctg ctcgccatca tctcgccgca gtaggcgctg
     1981 gcgagctgcg agatggccgt ctgctgtgcg gagccgaagg cggcaatcag cgggcctgcc
     2041 ggcatcgact gctgcgacgc ctggtagagc gggttcacca cactgtcggt gaccgccacc
     2101 ccggtgatgc gcgcgagcga gtggttgaca cgctcgaaag tcgccacacc gaagtccggc
     2161 tgcggcgccg agggcggcgt cggcggcgtc cccggcgggg tgggatcgac gtacgcgtgc
     2221 acgtgcgtgc cgatctggtc gaacgacagg aagaacaggt cgttcgcggg cccgagcgtc
     2281 gccggcacca ccgtgccgag cggcgagagc agctggccgt tggcggccgt gtagttcgag
     2341 ccgccgacgc tcgaagccac cgaggtgaag gcctgacccg cgggcgcgag taccccgttg
     2401 acaccgatgc gtatgccgct gatcggcaga ttggccggca ttgcgttcgc gtccaggctg
     2461 atgaacttcg gctgcgcgaa caggtagctg tagctgtcgt actggctgcc ctggaacagg
     2521 atgtacgact ggttcacgcc ggtcagctga ctgactccga acagcaggaa gaacttctga
     2581 ccgacgcccg cggcgaagtt ctgctgtatc tgcgcggcag taagggcgcg attgtggatg
     2641 gcgacgaact tgatgacgcc ctggaactgc cgctgcccgg tggtctcgtt gccgagcacc
     2701 agcgcgaagg tgctgtccca gttggcgagc gagccgccct tggacgggtc ggcatcgccg
     2761 gtgtacacgc cgttgacgta cagcttctgg ccggcgatcg ggtcataggt gagcaccacg
     2821 tgctgcagag cggcctgcgc cgcgccgccg ttggtggtcg tgatgagcgg cggtgtgccg
     2881 gcggtgctgg tggtgctgct gcgcgcaaag ccctggtact gcatggcgtc ctggccgagg
     2941 gtcatgttgc gcgtggtgtt gctgccggag tagctgacga tccaggcgtt ggtctgcgtg
     3001 acattcgccg gcgccaccca ggtctcgatg gtgtactcgc ccgccggctg gatcatgccg
     3061 gcgaacttgg cgctcgcgcg ggtggaagcc tgcgccttgc cgcctgcacc gatgtggatg
     3121 ccccagccgc ctacccagct gacattgccc gagagcgaca gg
//
LOCUS       DATDMP010000040         3347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_340106, whole genome shotgun sequence.
ACCESSION   DATDMP010000040 DATDMP010000000
VERSION     DATDMP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340106"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VI195_00725"
     CDS             complement(<1..1930)
                     /locus_tag="VI195_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6822935.1"
                     /translation="MKSHVRVLVVGGGAVGVSTLYHLTRKGWTDVALIERTELTAGST
                     WHAAGLLPLFNMSYTVGQLHKYSVDLYKRLPAETGQDVSFHVTGNLRLATCRERMDEY
                     YKYCGTANTIGVPFEVITPRRVKELWPLIELGGDGDTPAIIGALYHPDDGHIAPADLT
                     MALRKGARAAGAEIYEHTEAVGIERTAAGEWRVRTGQGGDITAEHLVLATGNYARQTG
                     RLLGLNVPAIPVEHQYIVYDESPELKTYRQRGGRELAVLRESDESYYLREERMGWILG
                     PYERGAPARFADGVPEWFGRSLFPGDLDRLVPHVEAATRRVPALEHCGIKDIVNGPIS
                     YTPDGSPLIGPAWDVRNVWLNEGHSFGITAAGGAGWQLAEWIVEGEPGIDMLAVDPRR
                     FGSYTSKRYVVKKNEETYRNVFTVHFPDEERPEARPAKTSPVYDKLSARGAVWGQRYG
                     WERANWFAPPGVARRDEWSFRRSNYFEHVGNEARLMRERVGVIDLTPFTKHEVKGPGA
                     ESWLDRLVANKVPAKTGRMALSHALTTRGGIRSEFTITKIGDDHYYVVSAGAAERYDS
                     DYLFKSQPPDGSVALRNITNTRGCFVLAGPRARDVLAQLTDTPLDNSAFPWLTAQVIE
                     AGLAVDVYALRVNFVGSLG"
     gene            complement(1966..2514)
                     /locus_tag="VI195_00730"
     CDS             complement(1966..2514)
                     /locus_tag="VI195_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEY6822936.1"
                     /translation="MPDLRAMRIPPQLGTTSALESLPPAARYILRGGEAVRAAAEAAA
                     GVGFSAQPCRAMTRGERAALWLGPDEWLLIAPPAEGAALAVSLEAALASIPHSLVDVG
                     HRQSGCELRGPQAPALLSAGCPLDLDESAFPIGMCTRTVLAKAEVVLWRRAAHTFRLE
                     VARSFVAYVAQFLAEAARSGTD"
     gene            complement(2507..>3347)
                     /locus_tag="VI195_00735"
     CDS             complement(2507..>3347)
                     /locus_tag="VI195_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY6822937.1"
                     /translation="SDRFHVTTTTGGAGRVLAMMEDYLQTEWSDLSVWLTSTTEQWAV
                     IALQGPNSRRILAGLVDVDIGAAALPHMGAVCGHVCGVPMRLFRVSFTGELGFEINVP
                     ADFGTGVWERVWAAGEPQGLTAYGTETMHVLRAEKGYIIVGQDTDGTVTPEDVGLAWA
                     IGRDKADFVGKRSLQRPALRSPERRQLVGLLTANPQRVLEEGAQVLSGPDPQPPVRPL
                     GHVTSSYHSATLGRSVAMALIARGRARVGESLYGPLESGEVVAAQVASPVFYDPKGAR
                     LNA"
BASE COUNT          538 a         1139 c         1119 g          551 t
ORIGIN      
        1 agcccagaga accgacgaag ttcacccgca gcgcgtagac atccaccgcg agtcccgctt
       61 cgatcacctg cgcggtgagc caggggaagg cgctgttgtc gagtggcgtg tcggtaagct
      121 gtgcaagcac atcgcgtgct ctgggtcctg cgagcacaaa gcaaccgcgc gtgttggtga
      181 tgttgcgcag cgcgaccgag ccgtccggcg gctgcgattt gaacaggtag tcgctgtcgt
      241 agcgctcggc ggcaccggca ctgacgacgt aataatggtc atcgcctatc ttggtgatgg
      301 tgaattcgga gcggatgccc ccgcgcgtcg tgagcgcatg cgatagcgcc atccgcccag
      361 tcttcgccgg caccttgttg gcgaccagcc gatcgagcca gctctcggcg ccgggacctt
      421 tcacctcgtg cttggtgaaa ggtgtcagat cgatcactcc gacacgctcg cgcatgaggc
      481 gtgcctcgtt gccgacgtgc tcgaaatagt tgctgcgccg gaaactccac tcgtcgcggc
      541 gtgccacccc aggcggagcg aaccagttgg ctcgctccca gccgtaacgc tggccccaca
      601 ccgcaccgcg ggcgctcagc ttgtcgtaca ccgggctcgt ctttgctggc cgggcctcgg
      661 ggcgctcctc gtcggggaaa tgcaccgtga agacgttgcg ataggtttcc tcgttcttct
      721 tcaccacata gcgcttgctg gtgtaggagc cgaagcggcg cggatccacg gccagcatgt
      781 cgatgcctgg ctcgccctcg acgatccact cggcgagctg ccagcccgct ccgcccgcgg
      841 ccgtgattcc gaagctgtgt ccctcgttga gccagacgtt gcgcacatcc caggcgggtc
      901 caatgagggg gctgccgtcg ggtgtatagg agatcggacc gttgacgatg tccttgatgc
      961 cgcagtgctc gagcgcgggt acgcggcgcg tggctgcctc gacgtgcggc acgagacgat
     1021 cgagatcgcc ggggaagagg ctgcgcccga accactccgg cacaccgtcg gcgaagcgtg
     1081 cgggcgcgcc gcgctcataa gggccgagaa tccagcccat gcgctcctcg cgcaggtaat
     1141 aggactcatc cgactcgcgc agcacggcaa gctcgcgacc gcctcgctgc cgataggttt
     1201 tgagctccgg cgattcgtcg tagacgatgt actggtgctc gaccgggatc gcggggacat
     1261 tcagcccgag cagtcgcccc gtttgccggg cgtagttgcc ggtggcaagc acgaggtgct
     1321 cggcggtgat gtcgccgccc tggccagtgc gtacccgcca ttcgcccgct gccgtacgct
     1381 cgatgccgac cgcctcggtg tgctcgtaaa tctcggcgcc ggcagcgcgt gcgcccttgc
     1441 gcagcgccat ggtgaggtct gccggcgcga tatggccgtc atccgggtgg tagagcgcgc
     1501 cgatgatggc gggagtgtcg ccgtcgccgc cgagctcgat gagcggccac agctccttta
     1561 cgcgccgtgg tgtgatcacc tcgaagggca cgccgatcgt gttggcggtg ccacagtact
     1621 tgtagtactc gtccatccgc tcgcgacagg tcgcgaggcg cagattcccc gtgacgtgga
     1681 agctcacgtc ctgacccgtt tcggccggca gccgcttgta caggtcgacc gaatatttgt
     1741 ggagctgccc gacggtgtag ctcatgttga agagcggcag gagcccggcg gcatgccagg
     1801 tggacccggc ggtgagctcg gtgcgctcga tcagcgcgac atcggtccag cctttcctgg
     1861 tcaggtgata gagcgtgctc acaccgaccg cgccgccacc gacgaccagg acccgtacat
     1921 gcgatttcac gagagtctct ccagttgcac cggcgcggcc cgatgtcaat ccgtgccgct
     1981 gcgcgcggcc tcggcaagaa actgcgctac gtatgccacg aaggagcgcg ccacttcgag
     2041 acggaaagtg tgtgcggccc tgcgccacag caccacttcg gccttcgcca gcacggtacg
     2101 cgtacacatg ccgatgggga aggcgctctc atcgagatcg agcgggcacc ccgcggacaa
     2161 cagcgccggg gcttgcggac cgcgcagctc gcagccgctc tggcggtggc ccacatcgac
     2221 cagcgagtgg gggatgctcg ccagcgccgc ttccagcgag actgccaatg cggccccctc
     2281 tgccggcggc gcgatgagca gccactcatc ggggcccagc cagagcgccg cgcgctcacc
     2341 tcgcgtcatg gcgcggcagg gctgggcgga gaaacccacg cccgctgcgg cctccgcggc
     2401 cgcgcgtacc gcttcgccgc cacgcaggat gtaacgggca gccggcggca gggactcgag
     2461 cgcgctcgtc gtgccgagct gcggcgggat gcgcatggcc ctgagatcag gcattgagac
     2521 gcgctccctt cggatcgtag aacaccggcg aggcgacctg cgcggccacg acctcaccgc
     2581 tttcgagggg accgtagaga gattcgccca cgcgtgcacg gccgcgcgcg atcaacgcca
     2641 tggcaaccga tcggccgagc gtcgcactgt ggtaggaaga ggtcacgtgc ccgagcgggc
     2701 gcaccggtgg ctgcggatct ggcccgctca gcacctgcgc gccttcctcg agcacccgtt
     2761 gcgggttggc agtgagaagc cccacgagct gccgccgctc gggcgaccgc agcgccggtc
     2821 gttgcagcga gcgcttgccg acgaagtccg ccttgtcgcg gccgatcgcc caggcgagac
     2881 ccacatcctc gggggttacc gttccatcgg tgtcctggcc gacgatgatg tagcccttct
     2941 ctgcacgcag cacgtgcatc gtctcggtgc cgtacgccgt gaggccctgc ggctcgccag
     3001 ccgcccagac tctttcccac acgccggtgc caaagtccgc gggaacgttg atctcgaagc
     3061 cgagctcgcc ggtgaagctc acacgaaaga gccgcatcgg caccccgcag acatgaccgc
     3121 acacggctcc catgtgcggc agcgctgcgg caccgatgtc cacgtccacg aggccggcga
     3181 ggatccgtcg cgagttcggg ccctgcaggg cgatcaccgc ccactgctcg gtggtcgagg
     3241 tgagccatac ggagaggtcc gaccactcgg tctggagata atcttccatc atcgcgagca
     3301 cccggcccgc gccgccggtg gtggtcgtca cgtgaaagcg gtcggag
//
LOCUS       DATDMP010000041         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2006085, whole genome shotgun sequence.
ACCESSION   DATDMP010000041 DATDMP010000000
VERSION     DATDMP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006085"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VI195_00740"
     CDS             <1..1336
                     /locus_tag="VI195_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY6822938.1"
                     /translation="QYDLFHAYTVDEHTLFVVSNLRRFALARYDHEFPLCSRIMQALP
                     KPEVAYLAALFHDIAKGRGGDHSELGAVDAEAFCLEQGLSPYDARLTAWLVRHHLLLS
                     ITSQKQDIGDPQVINAFARTVGDETHLDYLYVLTCADVRATNPKLWNSWKATLFHDFY
                     KRVKQALRRGLESPIDAEHLVRETQDAARRLLFERGVAEADIVAVWTRFTAGYFLQHS
                     PEEVAWHTQLLSERDAASDEPLVALDPRSVRGTTAALIFARPRRHGFARTTAALDQLG
                     LNIVDARITPSGDGFSLDLYHLLEDDGAPITDSDRLAEIEHALWRSLQGAPDAPFAVS
                     RRAPRQARMFHTPTHILISVDERNRRSVLELTAGDRPGLLCEVGAVLMQERIELHAAK
                     IMTVGERAEDVFYLTDFDNRPLSAAAAQQLKERLIQVLDERQAALRSGGAGG"
     gene            1349..>2106
                     /locus_tag="VI195_00745"
     CDS             1349..>2106
                     /locus_tag="VI195_00745"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY6822939.1"
                     /translation="MSAPELERIKAIIEAAFEQRATLSSQHLPRELDGALEECLERLD
                     RGELRVAEPAAGGWVVNEWLKKAVLLSFRVRENRLVDAGFTRFYDKVPLKYAATSEQE
                     LRAGGVRIVPHALVRRGAYVAPGVVLMPCYVNIGAWIGAGTMVDTWATVGSCAQIGRN
                     VHLSGGVGIGGVLEPLQAAPTIIEDDCFIGARSEVVEGVVVGAGSVISMGVFLGQSTR
                     IYDRTTGTTLQGQVPPGSVVVPGTLPSSDGRYAL"
BASE COUNT          328 a          709 c          716 g          353 t
ORIGIN      
        1 gcagtacgac ctgttccacg cctacaccgt ggatgagcac acgttgttcg tggtgagcaa
       61 cctgcgccgc ttcgcgctcg cccgctatga ccatgagttc ccgctgtgca gccgcatcat
      121 gcaggcgctg ccgaaacccg aggtggccta cctcgccgca ttgttccacg acatcgccaa
      181 gggccgcggc ggtgaccatt ccgagctcgg cgccgtggat gccgaggcct tctgcctcga
      241 gcagggcctc tcgccctacg atgcgcgcct caccgcctgg ctggtgcgcc accatctgct
      301 tttatccatc acctcgcaga agcaggacat cggcgatccg caggtcatca acgctttcgc
      361 gcgcacggtg ggcgatgaga cacacctcga ctatctctat gtgctcacct gtgcagacgt
      421 acgcgcgacc aacccgaagc tgtggaactc gtggaaagcg acgctgttcc acgacttcta
      481 taagcgcgtg aagcaggcgc tgcgccgcgg cctcgagagc cccatcgatg ccgagcatct
      541 ggtgcgcgag acccaggacg ccgcgcgccg cctgctgttc gaacgcgggg tggcggaagc
      601 ggacatcgtt gcggtgtgga cccgcttcac cgcgggctat ttcctgcagc actcgcccga
      661 ggaggtcgcg tggcacacgc agctgctgtc cgagcgtgat gccgcctccg atgaaccgtt
      721 ggtagcgctc gatccccgca gtgtgcgtgg caccaccgct gcgctgatct ttgcacggcc
      781 gcgccggcac gggttcgcgc gcacgaccgc cgccctcgac cagcttggac tgaacatcgt
      841 ggacgcgcgc atcaccccga gcggcgacgg cttcagcctc gatctctacc atctgctcga
      901 ggacgatggc gcgccgatca ccgacagcga ccggctagcg gagatcgagc atgccttgtg
      961 gcgctcgctg cagggcgcgc cggatgcgcc gttcgccgtg tcgcggcgcg cgccacgcca
     1021 ggcgcgcatg ttccacactc cgacacacat tctcatcagc gtcgatgagc gtaaccggcg
     1081 ctcggtgctc gagctcaccg ccggcgaccg gccgggcctg ctgtgcgagg tcggcgcagt
     1141 gctgatgcag gagcgcatcg aattgcacgc cgcgaagatc atgacggtcg gcgagcgcgc
     1201 ggaggatgtg ttttacctca ccgacttcga caaccgcccg ctgagtgccg ccgccgcaca
     1261 gcagctgaaa gagcgcctga tccaggtgct cgatgagcgc caggcggcgc tgcgcagcgg
     1321 tggtgccggt ggctgagcgg ctgcgacgat gagcgcgccg gagctcgagc ggatcaaagc
     1381 catcatcgag gcggccttcg agcagcgcgc cacgctcagc agccagcatc tgccgcgcga
     1441 gctcgatggc gcgctggagg agtgcctcga gcggctcgat cgcggcgagt tgcgcgtggc
     1501 ggagccggct gcgggcggct gggtggtgaa tgagtggctg aagaaggcgg tgctgctctc
     1561 cttccgtgta cgcgagaacc gcctggtgga cgctggattc acgcgtttct acgacaaggt
     1621 gccgctcaaa tacgccgcca cgagcgagca ggagttacgc gctggcggag tgcgcatcgt
     1681 gccgcacgcg ctcgtgcgcc gcggcgccta cgtcgcgccc ggtgtggtgc tcatgccgtg
     1741 ctacgtgaac atcggcgcct ggatcggcgc gggcacgatg gtcgacacct gggcgaccgt
     1801 aggctcgtgt gcgcagatcg gccgcaacgt gcatctctcc ggcggtgtgg ggatcggcgg
     1861 ggtgctcgag ccgttgcagg ccgcccccac catcatcgag gacgactgct tcatcggcgc
     1921 ccgttcggaa gtcgtcgagg gagtggtggt gggagcgggc tcggtgatct ccatgggtgt
     1981 cttcctcggc cagagcacgc gtatctacga ccgcaccacg ggcactaccc tccagggcca
     2041 ggtgccgccg gggtcggtgg tggtgccggg caccctgcca tcgagcgatg gccgctacgc
     2101 gcttta
//
LOCUS       DATDMP010000042         3492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_895634, whole genome shotgun sequence.
ACCESSION   DATDMP010000042 DATDMP010000000
VERSION     DATDMP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895634"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2203
                     /gene="alaS"
                     /locus_tag="VI195_00750"
     CDS             <1..2203
                     /gene="alaS"
                     /locus_tag="VI195_00750"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY6822940.1"
                     /translation="ADVWLKDIGISPERFSRLGESSNFWAMGDTGPCGPCSEIFFDHG
                     PAVAGGPPGSKDEEGDRYVEIWNLVFMQYERAADGTLTPLPKPSVDTGMGLERITAVM
                     QGVHSNYDIDLFKNLIRAAGKLAGTSVLSSSSLRVIADHIRASAFLIVDGVSPSNEGR
                     GYVLRRIIRRAIRHGYKLGIQEPFFYKLVRPLEQEMGAAYPELTRGRAHAERVLKQEE
                     ERFAQTLANGMVLLETAIRNLRGAKVIDGDTVFKLYDTYGFPVDLTADVARERGLAID
                     QARFDAAMEEQRRRSQERSKFNVDLSSGTTPEGRTLFQGYEGLSDSGNVVALLKKGAA
                     VETLEAGDAGEVVLDRTPFYAEAGGQVGDAGQLTSSGMRFLVSDTQKRGATHAHIGEL
                     LEGRIRVGDNLEAEVDGARRQATALNHTATHLLHAALRKVLGAHVLQKGSLVAPDRLR
                     FDFAHFQALTGGELEEIERLVNAQIRANLPAETTVMDYEGAVAAGAMALFGEKYDKDV
                     RVLRLGDFSMELCGGTHVRRAGDIGLFKIVGESGVAAGVRRIEALTGEGALAYIEQNE
                     ALLKDLAALVRGSRDDLADKVRDQLERTRQLEREVRTLKERLASGKGVDLAASAVDVQ
                     GVKVVATKVDGADAGALRSAVDQLKARLKSAVVVLASVEDAAKVLLVAGVTPDQTSRI
                     QAGELAGAVAALVGGRGGGRADFAQAGGSKPEALDAALESVQEFVRARLTA"
     gene            2327..2417
                     /locus_tag="VI195_00755"
     tRNA            2327..2417
                     /locus_tag="VI195_00755"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2361..2363,aa:Ser,seq:gct)
     gene            2521..>3492
                     /locus_tag="VI195_00760"
     CDS             2521..>3492
                     /locus_tag="VI195_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY6822941.1"
                     /translation="MNPIKYAAVALAALTLAMSVRAPANDEEHGHRIQHVLLISIDGM
                     HALDFINCAHGLSGIDNGAPYCPNLAALKHHGLNYLEASTSRPSDSFPGLMAIVTGGS
                     PRTFGAFYDVAYDRSLAPPSVMTGNGLAPGSCTPGVFNGTTTEYEEGIDKDQTFLNGV
                     GPGDGDGGVSSIDPGKLPRDPAHGCAPVYPWNFIRTNTVFGVIHESRGYTAWSDKHPA
                     YSSIAGPNVHGPANVPENLDDYFSPEINSVPVPLPFPGCSAADDPNFISLGAYTDSFA
                     FTRCYDTLKVNGVLNQIDGFRHDRSGRAPVPTIFGMNFQAVSVGQKLI"
BASE COUNT          607 a         1137 c         1162 g          586 t
ORIGIN      
        1 ggcggatgtc tggctgaagg acatcggcat aagcccggag cgcttttcgc gcctcggtga
       61 gagctcgaac ttctgggcca tgggcgatac cgggccctgt ggcccctgca gcgagatttt
      121 cttcgatcac ggacctgccg tagcgggcgg cccgcccggg tcgaaggatg aggaaggtga
      181 ccgttacgtc gagatctgga atctggtgtt catgcagtac gagcgcgcgg cggatggcac
      241 cctgaccccg ctgccgaagc catcggtcga caccggcatg ggcctcgagc gcatcacggc
      301 ggtgatgcag ggcgtgcact ccaattacga catcgacctc ttcaaaaatc tcatccgcgc
      361 cgcgggcaag ctcgcgggca cctcggttct gagctctagc tcgctgcgcg tcattgccga
      421 tcacattcgt gccagtgcct ttctcatcgt ggatggcgtg tcgccctcca acgaggggcg
      481 cggctacgtg ctgcgccgga tcatccgtcg tgccatccgg cacggctaca agctgggtat
      541 ccaggagccg ttcttctata agctggtgcg cccgctcgag caggagatgg gcgcggcata
      601 cccggagctg acgcgcggcc gcgcgcacgc ggagcgtgtc ctcaagcagg aggaggagcg
      661 cttcgcacag acactcgcca atggcatggt gctgctcgag accgccatcc gcaacctgcg
      721 cggtgcgaag gtcatcgatg gcgatacggt gttcaagctc tatgacacct acggttttcc
      781 ggtcgatctc accgcggacg tggctcgaga gcgtgggctg gcgatcgacc aggcgcgttt
      841 cgatgccgcc atggaagagc agcgacgccg ctcgcaggag cgcagcaagt tcaacgtcga
      901 cctcagcagc gggaccacgc ccgagggccg cacgctgttc cagggctacg agggcttgag
      961 cgattccggc aacgtggtgg cgctgctgaa gaagggcgca gcggtcgaga ctctcgaagc
     1021 cggagatgcg ggtgaggtgg tgctcgaccg caccccgttc tacgccgagg cgggcggcca
     1081 ggtcggtgat gccggccagc tgacctcgag cggaatgcgc ttcctcgtga gcgataccca
     1141 gaagcgcggc gcgactcacg cgcacattgg cgaactcctc gaaggccgca ttcgtgtcgg
     1201 cgacaatctc gaggcggaag tcgatggcgc gcgccgtcag gcaaccgcgc tcaaccacac
     1261 cgctacccac ctgctgcacg cggcgctgcg caaagtactc ggcgctcacg tgctgcagaa
     1321 gggctcgctc gtggcacctg accggctgcg cttcgatttc gcgcactttc aggcgctcac
     1381 tgggggagag ctcgaggaga tcgagcggct cgtgaacgcg cagatacgcg cgaacctgcc
     1441 ggcggaaacg acggtcatgg actacgaggg cgccgtggct gcgggtgcga tggcgctgtt
     1501 cggcgagaaa tacgacaagg acgtgcgcgt gctgcgactc ggtgacttct cgatggagct
     1561 gtgcggcggc acgcacgtgc gtcgcgccgg cgacatcggc ctgttcaaga tcgtgggcga
     1621 gagcggggtc gctgccggcg tacgccgcat cgaggcgctc acgggcgaag gcgcgctcgc
     1681 gtacatcgag cagaacgaag ccctgctgaa ggatctcgcg gcgctggtac gaggctcacg
     1741 cgatgacctt gccgacaagg tgcgcgacca gctcgagcgc acgcggcagc tggagcgcga
     1801 ggtacgcacg ctgaaagagc gcctcgcctc gggcaagggc gtggatctgg cggccagcgc
     1861 cgtcgacgta cagggcgtga aggtcgtggc caccaaggtc gatggcgccg atgccggggc
     1921 gctacgcagc gccgtggacc agctcaaggc gcgtctcaag tcggcggtcg tcgtgctggc
     1981 gagcgtggag gacgcggcga aggtgttgct agtggccggt gtcacacccg atcagacctc
     2041 gcgtatccag gccggagagc tcgccggcgc cgtcgctgcg ctggtcggcg gccgcggcgg
     2101 cggccgtgcg gacttcgccc aggcaggggg cagcaagccc gaggctctcg atgccgcgct
     2161 cgaatcggtg caggagttcg tacgggcgcg cctgacggcc tgaggcgccg ggcgcagcgg
     2221 cgcttttgcc gcgccggtgc ccgtttgagt atcatggccg ccccgcgcgc ggcgcgccgg
     2281 tgacaggcgc gccacaagtc tcaaaccgaa aagttgttcc cgcggcggag agatggccga
     2341 gtggtcgaag gcgcacccct gctaagggtg taagggtcaa aagcccttcg agggttcgaa
     2401 tccctctctc tccgccataa gacccggggg gcactccgta agcatgcggc agcacaaccg
     2461 taatgcgcgc gcaaccttgc ctgcctagtg tcgcgtctcc ggtcaacccc tggagtcgcc
     2521 atgaacccga tcaaatacgc ggcagtagcg ctcgctgctc tcaccctggc catgagtgtc
     2581 cgggcccctg cgaacgatga ggagcacggt caccgcatac aacacgtgct gctcatcagc
     2641 atcgacggta tgcatgcgct cgatttcata aactgcgcgc acggcctgag cggcatcgac
     2701 aacggcgccc cgtactgtcc taacctcgct gcgctcaagc accacggtct caactatctc
     2761 gaagcttcga cctcgcgacc ctctgattcc ttcccgggtc tgatggccat cgtcaccggc
     2821 ggatcgccgc gcaccttcgg tgcgttctat gatgtggctt acgaccgctc gctggctccg
     2881 ccgtctgtca tgaccggcaa cgggctcgct cccggctcat gtaccccggg tgtcttcaac
     2941 ggcactacca ccgaatacga ggaaggcatc gacaaggatc agaccttcct caatggcgtc
     3001 ggcccgggcg acggcgacgg tggcgtaagc tcgatcgacc ccggaaagtt gccgcgggac
     3061 ccggcgcatg gctgcgctcc cgtgtatccc tggaacttca tccgcaccaa cacggtgttc
     3121 ggtgtcatcc acgagtcgcg cggttacacg gcgtggtcgg acaagcaccc cgcttactcc
     3181 tcgatagccg ggcccaacgt ccatggcccg gcgaacgtcc cggagaatct cgacgactac
     3241 ttctcgcccg agatcaactc cgtgcccgtg ccgctgccgt ttccgggttg cagcgccgcc
     3301 gacgatccga acttcatcag cctcggcgct tacaccgata gctttgcatt cacgcgctgt
     3361 tacgacacgc tcaaggtgaa cggtgtgctc aaccagatcg atggcttcag gcatgaccgc
     3421 agcgggcggg cgccggtgcc gaccatcttc ggcatgaact tccaggcggt gagtgtcggc
     3481 cagaaactga tc
//
LOCUS       DATDMP010000043         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2006239, whole genome shotgun sequence.
ACCESSION   DATDMP010000043 DATDMP010000000
VERSION     DATDMP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006239"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VI195_00765"
     CDS             complement(<1..406)
                     /locus_tag="VI195_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822942.1"
                     /translation="MLRRFAVLLVMLVAIAIAVVLLMVCPWWALPAAVVLLAAWLAGT
                     RTGRQTWSVTGVGLATIPQRLGSSSVVVVGIAGVVGVLVAMLAMAVGFEKTLRQTGSD
                     GTAIVMRAGAQTELNSVLSHETAQLVAQEPQVL"
     gene            complement(425..1588)
                     /locus_tag="VI195_00770"
     CDS             complement(425..1588)
                     /locus_tag="VI195_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6822943.1"
                     /translation="MKYLHLVWAALMRRKTRTVFTLLSVLAAFLLFGLLDSVRSAFAA
                     ASRSVAGVDRLVTVSKVSFTLSLPKSLLGRIEAVPGVTAVTYANWFGGIYQDPKNFFP
                     SEAVAANFLALYPEWKLPEDELRAFHDTRTGAVVGASLARHFHWKLGDKIPLEATIFP
                     QKDGSNTWTFDLVGIYHVADRRLESQENALFFNWDYFDEARQFGNGNVGWYVTRIGNP
                     AEADRVAQAIDALSANSDHETKTQSEQAFNVAFVSQFADIGLIVGAIMGAVFFTLILL
                     TGNTMAQAVRERVPEIAVLKTIGFSNRSVLGLVLAEAAALLVLGGATGLALAGLVVAA
                     VRLKLGTAVPMNPVGADSWLRGLGLAVLIGLIVGALPALRGMRLRIVDALAGR"
     gene            complement(1585..2292)
                     /locus_tag="VI195_00775"
     CDS             complement(1585..2292)
                     /locus_tag="VI195_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6822944.1"
                     /translation="MDTLVVTRNLSKVYRRGRERLEVLHHVDLDIASGDFLALMGPSG
                     SGKTTLLNLIGGLDSPTEGTIEVGGRRIDTLGEGALARWRAAHVGFVFQFYNLLPMLT
                     ARRNVELPLLLTRLSAATRRRNAGIALELVGLGDRAGHKPGELSGGQQQRVAIARAIV
                     SDPTLLVCDEPTGDLDRQSAAEILTLLQQLNREHGKTIIMVTHDPKAAEYARHTLHLD
                     KGTLVEGAQRAAGEAAA"
     gene            complement(2307..>2511)
                     /locus_tag="VI195_00780"
     CDS             complement(2307..>2511)
                     /locus_tag="VI195_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822945.1"
                     /translation="PGTAVVERAGKPVVFAIEGERARVRAVTPGQNYGDLRLVEGLPG
                     GTRVVRAPPAALSDGARVAVAKP"
BASE COUNT          380 a          921 c          854 g          356 t
ORIGIN      
        1 gcagtacctg cggttcctgc gccacgagct gcgcggtctc gtgactcagc accgagttga
       61 gctcggtctg cgccccggcg cgcatcacga tcgcggtgcc gtccgagccg gtctggcgca
      121 gcgtcttctc gaagccgacc gccatggcga gcatcgccac cagcacgccg accactccgg
      181 cgatgccgac gaccaccacc gacgaggacc cgagccgctg cggaatggtc gcgagcccca
      241 ccccggtgac cgaccaggtc tgccgtccgg tgcgcgtgcc ggcgagccat gccgcgagca
      301 gcaccaccgc ggcgggcagc gcccaccagg ggcacaccat gagcagcacc acggcgatgg
      361 cgatcgccac cagcatcacg agcagcaccg cgaatctgcg caacaccggc ttactccccg
      421 cgcctcaccg gcccgcgagc gcatcgacga tgcgcagccg catgccacgc agcgccggca
      481 gcgcgccgac gatgagcccg atcagcacgg cgagtccgag gccccgcagc cagctgtcgg
      541 cgccgaccgg gttcatcggc acggcggtgc cgagcttgag gcgcacggcc gcgaccacca
      601 ggccggcgag tgcgagcccg gtggcgccgc cgagcaccag cagcgccgcg gcttccgcca
      661 gcaccagccc gagcacgctg cgattggaga agccgatggt cttgagcacc gcgatctccg
      721 gcacccgctc gcgcaccgcc tgcgccatgg tgttgccggt gagcaggatg agcgtgaaga
      781 acaccgcgcc catgatggcg ccgacgatca ggccgatgtc ggcgaactgg ctgacgaaag
      841 cgacgttgaa ggcctgctcg ctctgcgtct tggtctcgtg gtcggaattc gccgacagcg
      901 catcgatcgc ctgcgccacc cggtccgcct cggcggggtt gccgatgcgc gtgacgtacc
      961 agcccacgtt gccgttgccg aactgccgcg cctcgtcgaa gtagtcccag ttgaagaaca
     1021 gcgcgttctc ctggctctcg agccggcgat cggcgacgtg gtagatgccg accaggtcga
     1081 aggtccaggt gttcgagccg tccttctgcg ggaagatggt cgcctcgagc gggatcttgt
     1141 cgccgagctt ccagtggaag tgcctggcga gactcgcgcc gaccaccgct ccggtgcggg
     1201 tgtcgtggaa ggccctgagc tcgtcttccg gcagcttcca ctccgggtac agcgcgagga
     1261 agtttgccgc caccgcttcg ctcgggaaga aattcttcgg gtcctggtaa atgccgccga
     1321 accagttcgc gtaggtgacg gccgtgacgc ccggcaccgc ctcgatgcgc ccgagcaggc
     1381 tcttcggcag cgacagcgtg aagctcacct tggagaccgt caccagccgg tccactccgg
     1441 cgacgctgcg gctggcggcg gcgaaggcgc tgcgcaccga atcgagcagc ccgaacagca
     1501 ggaacgccgc cagcaccgac agcagcgtga acaccgtgcg ggtcttgcgc cgcatgagcg
     1561 ccgcccatac cagatgcagg tacttcacgc ggccgcctcg ccggcggcgc gctgcgcgcc
     1621 ctcgaccagc gtgcccttgt cgaggtgcag cgtatggcgc gcgtactcgg ccgccttcgg
     1681 atcgtgcgtc accatgatga tggtcttgcc gtgctcgcgg ttgagctgct gcagcagcgt
     1741 caggatctcc gccgccgact ggcggtcgag atccccggtc ggctcgtcgc acaccagcag
     1801 cgtcgggtcg gagacgatcg cgcgcgcgat cgcgacgcgc tgctgctgcc cgccggagag
     1861 ctcgcccggc ttgtggcccg cgcggtcccc gagcccgacc agctcgagag cgatgccggc
     1921 gttgcggcgg cgggtcgccg ccgacaggcg cgtcagcagc agcggcagct ccacgttgcg
     1981 gcgcgccgtg agcatcggca gcaggttata gaactggaac acgaagccga cgtgggcggc
     2041 gcgccagcgc gcgagcgccc cctcgcccag cgtgtcgatg cgcctgcccc ccacctcgat
     2101 cgtgccctcg gtgggggagt cgaggccgcc gatcaggttg agcagcgtcg tcttgcccga
     2161 gcccgacggc cccatgagcg cgagaaagtc gccgctggcg atgtcgagat cgacgtgatg
     2221 cagcacttcg agccgctcgc ggccgcgccg gtagaccttg ctcaggttgc gggttacgac
     2281 cagtgtgtcc acgcttgtcc cctgcttcag ggtttggcga ccgcgacgcg tgcgccgtcg
     2341 ctcagggcgg ccggcggcgc gcgcaccacg cgcgtcccgc ccgggagtcc ctcgaccagc
     2401 cgcaggtctc catagttctg acccggtgtc accgcccgca cgcgcgcccg ctcgccctcg
     2461 atggcgaaca cgaccggctt gccggcacgc tcgaccacgg cggtgccggg c
//
LOCUS       DATDMP010000044         3743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_229702, whole genome shotgun sequence.
ACCESSION   DATDMP010000044 DATDMP010000000
VERSION     DATDMP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229702"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VI195_00785"
     CDS             <1..896
                     /locus_tag="VI195_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6822946.1"
                     /translation="PKTWWASVRQSRATIVHYLGVMPAILLSVPPSPQDRAHAVRFGF
                     GAGVNPRHHAAFEARFGFPLIEAWAMTETGAGALLIANREPRHVGTSSIGAATADIEC
                     RVIDEHGTEVATGESGELLVRHAGAEPRFGFFRNYLKDEEATKEAWRDGWFHTGDLVS
                     RDAHGVFRFVDRRKNIIRRSGENISAVEVEAVLLQHPAVGNAAVTAVPDELRGDEVFA
                     CIVPRESVADPARLAQELGTLCRERLAYYKAPGYVAFCTALPLTRTEKLQRSELRELA
                     RRLLEQGSCIDLRGLKRREAQ"
     gene            893..2062
                     /locus_tag="VI195_00790"
     CDS             893..2062
                     /locus_tag="VI195_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY6822947.1"
                     /translation="MSRRAYEGVAVAVPVTVPYVRYSVRSAHWFIARALRALLERAGL
                     QKGQIDGACISGFLLFPDTAVGLMQHLGLSPRWLDHIPMGGASGVVALRRAARAVQSG
                     DAEIVACIAGDTNHVDSFRHTIASFSQFARDAVYPYGSGGPNASFAFLTDHFMRTRSA
                     EREDFGKLCIAQRQNALSFPHALFKKPLSMEEYLAARPIAEPLHLFDCVMPCAGAEGL
                     LVMSEARARALELPFARVLATIERHNAFPDDPIQVRGGWALERDSLWNQAALGPKDMD
                     LVELYDDYPVITAMQLEDLGFCATGEVVQLIRDNTFTTEGSLPLNTSGGQLSVGQAGA
                     AGGYLGLVECLRQLTGQALGARVTDARRGLVSGFGMINYDRGLCTGAAVLEAGLG"
     gene            2059..3306
                     /locus_tag="VI195_00795"
     CDS             2059..3306
                     /locus_tag="VI195_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6822948.1"
                     /translation="MTEPIRRPRRKNPVLRTPLPVLPPWPRSRIALGLTAAAAEGRFA
                     LQVCAECGAVQYPPREACHRCLSDRLPWREQSGEGELISQTLLRHSHDIFFRERVPWR
                     LGMVQLDAGPTVVVHLHGAVGAPPARVRVGARLDKAGMGVLVGFPEKDVPHMADDRQL
                     REMTCDPKFRKVLITDGKTRVGQALARALVGAGADLVWVGHAEPWKQLPGLAELEDLP
                     QVSLVLLDVTDARSVKEAAGEFGAKVDILINNVEVHRTHGITSRYGTDVARAEIEANY
                     LGLLRLAQEFGPVMRARGADGQSSAVAWVNLLSIFALSNYPAHGTYSASKAAALSLAQ
                     CLRAEMRPAGLRVINIFPGPIEDEWNQLLPPPKLAPEAVARAVVAALKDGVEDVYPGD
                     VAQEWLARWRESPKVLERELAAQ"
     gene            3325..>3743
                     /locus_tag="VI195_00800"
     CDS             3325..>3743
                     /locus_tag="VI195_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6822949.1"
                     /translation="MNDRRAIVTGGSTGIGLAIVSALIERGYEVISLARRPAALKSVR
                     LRSVQVDLADVEATRAAVAEIASREAVTTIVHNAGAILEKPIEEVRAADIDTLAHLHL
                     TAALTLLQANLPAMRAARFGRIVLISSRAVLGLAKRTA"
BASE COUNT          605 a         1205 c         1298 g          635 t
ORIGIN      
        1 acccgaagac ctggtgggcg agcgtgcgtc agtcgcgcgc cacgatcgtg cattatctcg
       61 gggtgatgcc cgcgattctc ctgtccgttc cgccctctcc acaggaccga gcgcatgcag
      121 tgcgtttcgg cttcggcgcg ggggtaaacc cccggcacca tgccgccttc gaggcacgct
      181 ttggcttccc gttaatcgaa gcatgggcaa tgaccgagac gggcgcgggc gctttgctga
      241 tcgccaatcg cgagccgcgc catgtcggga cctcctctat cggggcggcg accgcggaca
      301 ttgagtgccg cgtaatcgac gagcacggta cggaagttgc gaccggcgag agcggcgagt
      361 tgctcgtgcg ccatgcgggt gccgagcctc ggttcgggtt ctttcggaat tatctgaagg
      421 atgaggaggc gaccaaggag gcttggcgcg acggttggtt tcataccggg gacctggtga
      481 gccgggacgc gcacggcgtg ttccgattcg tcgaccggcg caagaacatc atccgcagaa
      541 gtggcgagaa catctcagcg gtcgaggtcg aggcggtcct gttacagcac cccgcggtgg
      601 gcaacgcggc cgtgactgcg gtaccggacg agctgcgcgg agatgaggtg tttgcctgta
      661 tcgtgccccg ggagagcgtc gcagatcctg cacgcctcgc gcaggagctc ggtaccttgt
      721 gccgcgagcg gctcgcttat tacaaggcgc cgggttacgt cgccttctgt acggctctgc
      781 cgctcacacg caccgaaaaa ctccagcgca gcgagctgcg cgagctcgca cgacgcctcc
      841 tcgagcaggg gtcctgcatc gatctccggg ggctgaagcg tcgggaggcg cagtgagccg
      901 acgggcctac gagggcgtgg cggtcgccgt cccggtgacg gtgccctacg tgcgctactc
      961 ggtgaggagc gcgcactggt tcatcgccag agcgctgagg gcgctcctcg agcgtgccgg
     1021 tctgcagaag gggcagatcg atggcgcgtg catctccggg ttcctgctct tcccagacac
     1081 ggccgtcggc ctcatgcagc acctcggcct ctccccgcgc tggctcgatc acatccccat
     1141 gggcggggca agcggggtgg tggcgctgcg gcgcgcggcg cgcgcggtgc agtcgggcga
     1201 tgcggagatc gtggcctgca tcgccggtga cacgaatcac gtcgactcct tccgtcacac
     1261 cattgcgagc ttcagccagt tcgcgcgcga tgcggtctac ccttacggct cgggaggccc
     1321 gaacgcgagc tttgccttcc tcaccgacca cttcatgcgc acacgcagcg ccgaacgcga
     1381 ggacttcggc aagctgtgca tcgcgcagcg gcagaacgcc ctctcctttc cgcatgcgct
     1441 cttcaagaag cccctttcaa tggaggagta cctcgcggcc cgaccgatcg ccgagccgct
     1501 gcatctgttc gattgcgtga tgccctgcgc aggcgccgaa gggctcctgg tcatgagtga
     1561 ggcgcgggcg cgggcgctcg agctgccttt cgcacgagtg ctggccacca tcgagcgcca
     1621 caacgccttt cccgatgacc ccatccaggt gcgcggcgga tgggcactcg agcgcgactc
     1681 gttgtggaat caggcggcgc ttggacccaa ggacatggac ctggtcgagc tctacgacga
     1741 ctacccggtc atcacggcga tgcagctcga ggacctcggt ttctgcgcca ccggcgaggt
     1801 ggtgcagctc atccgcgaca acaccttcac caccgaggga tcactgccgc tgaatacctc
     1861 gggcggccaa ctctcggtcg ggcaggcggg cgccgcgggt ggctacctgg gtctagtcga
     1921 atgtttgcgg cagctgaccg gtcaagcgct cggggcacgc gtcacggatg cgcgacgggg
     1981 cctcgtcagc ggcttcggta tgatcaacta cgaccggggc ctgtgtactg gtgcggctgt
     2041 gctcgaggcg ggcctcggat gaccgagcca atccggcggc cgcggcgcaa gaacccggtg
     2101 ctgcgtacgc ctctgccggt gttgccacca tggccgcgca gccgcattgc cctcggcctc
     2161 acggcggctg cggccgaggg acgcttcgcg ctgcaggtat gtgcagagtg cggtgcggtt
     2221 caatacccgc cacgcgaggc ctgccatcgg tgtctcagcg atcggctccc ctggagagag
     2281 cagtccgggg agggtgagct gatctcgcaa acgctcctgc gccacagtca cgacatcttc
     2341 tttcgcgagc gcgtgccctg gcgcctcggc atggtgcaac tcgacgcggg gccgaccgtg
     2401 gtggtgcacc tgcacggggc ggtgggcgca cccccggcgc gcgtgcgcgt cggtgcccgc
     2461 ctggataagg ccggtatggg cgtgctcgtt ggttttccgg agaaggacgt acctcacatg
     2521 gcagacgaca gacagctgcg cgaaatgacc tgtgatccca aattccgcaa agtcctcatc
     2581 acggacggca aaacacgggt agggcaagcg ctggcgcgcg cactcgtcgg cgcgggcgcg
     2641 gacctggtat gggtcggcca cgccgagccg tggaaacagc ttcccgggct ggcggagttg
     2701 gaggacctgc cgcaggtgag cctggtactg ctggatgtca ccgacgcgcg ctcggtcaag
     2761 gaggccgccg gggagtttgg tgccaaggtc gacatcctga tcaataacgt cgaagtgcac
     2821 cgtacgcacg gcatcacttc acgttacggc acggacgtcg cgcgcgccga gatagaggcg
     2881 aactacctgg gcttgttgcg tctcgcgcag gagttcggcc cagtgatgcg cgcacgcggc
     2941 gccgatgggc aatcgagtgc ggtggcgtgg gtcaacctgc tgtcgatctt tgcgctcagc
     3001 aactacccgg ctcacggcac ctactcggca tccaaggcgg cggcgctatc gctggcgcaa
     3061 tgtctgcgtg cggagatgcg ccccgcgggc ctgcgcgtca ttaacatctt ccccggaccc
     3121 atcgaggatg agtggaacca gctgctgccg ccgcccaaac tcgcccctga ggccgtggcg
     3181 cgcgcagtgg tcgcggcgct caaggacggg gtggaggatg tctaccccgg cgatgtggcg
     3241 caggagtggc tcgcgcgttg gcgcgagagt ccaaaggtgc tcgaacgcga gcttgccgca
     3301 cagtaatccg aaaggagaca ggcgatgaac gaccgcaggg cgatcgtcac tggcggcagc
     3361 acgggaatcg ggctcgcgat cgtgagcgca ttgatcgagc ggggctacga ggtgatttcg
     3421 ctcgcccgca ggccggcggc actgaagtct gtgcggctgc gctcggtaca ggttgacctg
     3481 gccgatgtcg aggctacgcg agctgcggtc gccgagatcg cttcgcgcga ggccgtcacg
     3541 accatcgtgc acaatgcagg cgccatcctt gagaaaccca tcgaggaagt gcgcgcggcg
     3601 gacatcgata cactcgcgca cttgcatctc acggctgcgc tgacgctgct gcaagcaaac
     3661 ctgccggcga tgcgtgccgc gcgcttcggg cgcatcgtgc tgatctcctc gcgcgcggtg
     3721 ctcgggctag cgaagcgaac cgc
//
LOCUS       DATDMP010000045         6605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_451994, whole genome shotgun sequence.
ACCESSION   DATDMP010000045 DATDMP010000000
VERSION     DATDMP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451994"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..519
                     /locus_tag="VI195_00805"
     CDS             247..519
                     /locus_tag="VI195_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY6822950.1"
                     /translation="MPKFVIERDIPGAGKLTPSELKSISQKSCGVLSSLGPSVQWIHS
                     YVTDDKIYCVYHAKDESLVRRHAELGGFPANRIARVMTIIDPGTSE"
     gene            565..1077
                     /locus_tag="VI195_00810"
     CDS             565..1077
                     /locus_tag="VI195_00810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY6822951.1"
                     /translation="MDATTLRSIQAPIKERYKGDAQAAQITLRAKGTLDGSRIACKVE
                     TGRALAVAGLHPATGGSGSELCSGDMLLEALVACAGVTLKAVATALEIPVRSATVCAE
                     GDLDFRGTLGVAKDARVGFGAIRLAFSVDSDAPQEKLDQLMKLTERYCVVYQTIRGGV
                     PVDVRMSRGA"
     regulatory      1263..1349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1644..2171
                     /locus_tag="VI195_00815"
     CDS             1644..2171
                     /locus_tag="VI195_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822952.1"
                     /translation="MRKHFLVVMSALLCICARPSPADENARAQMKGLDEQVQEVKSDV
                     LSIAAELNQLEEKLLYPSDTHLAVFVSMPHGETLRLDAVQIHIDGQLATHYIYSFKEL
                     DALRRGGVQRIYSGNIATGAHEIEVAVSGKLANGKDYSETERFPFTKEIKPKLIGVTF
                     AGPDGPQPGIHLGDW"
     gene            2175..4058
                     /locus_tag="VI195_00820"
     CDS             2175..4058
                     /locus_tag="VI195_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6822953.1"
                     /translation="MRRGKQWLSMGLALAAVAARPSPAADLRDLYFGEALYQAYQGQY
                     FDALERLDAELAQHHRVDEPQLDSLQYHIRQADFSVGDFELRYRMHLRAGRAIKAVLD
                     ADVEQPVKNEAAYRLARIEFQKDQPEDALQALERIQGTVPDDIKDDIEFLRANVYLAL
                     GRPADAVEVLKRVQSAQTLKGFAAYNLGIALLQAGHSPEGLRQLDKAGQIESHESATS
                     AIRDKSNMVLGSVMLRSEDYAHAQLAFDRVRLEGPYSNRALLSTGWAEASAHDYRKAL
                     VPWGMLIRRDPTDAAVQEARLALPFAYSKLQVYGRAALLYGQALDSFGKELEKVDAST
                     RSIRAGNFLKALVREEIKQDNVWVVRLRTLPDTPETFYLTELMASNDFQTALQNYLDM
                     EDLRRRLMAWQGGFDAFADLIRLRRANYEPLLPQVDAHFRELDAQMRVRLEQREHLQK
                     RLQDILTAPRPVLLATADERVALERLRKIETALKHASGPECAAQQVRLQHLQGVLTWR
                     LYTQYPERLTQAHKDLHDLNEDVRVLQERYTAYVRARQAAVHSYAGYDESIQRLRTRV
                     GEALERVNTLLARQGHVIEAVAIDELNTRRQRLEAYQVQARYAVADSYDRALKEHATG
                     GTP"
     gene            4055..>6605
                     /locus_tag="VI195_00825"
     CDS             4055..>6605
                     /locus_tag="VI195_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6822954.1"
                     /translation="MMRLVRTRAVAFLLPVIAACAHQADRSGTLATLHQVRPDLEEVP
                     VDQGLNRAVQSYGDFLKQAPDSALAPEAMRRLADLKIEKEFGLQGDGKLVELSASESA
                     VQGKAVAPGLGAAPATPAGSLGRDPAGALRAPAVTRIDARSATRSHAAAPGAGPAAAS
                     ERDLEQRAAQLGIPAADAMTPLALPAGMDGDAARAGPLEAIKLYDELLAKYPHYALRD
                     QVLYQKARAYEELGQTEDAMNVMEQLVQENPRSRFSDEVQFRRAERFFIARKYREAES
                     AYTAIVDGGSGSEYYEPALYKLGWTLYKQQLYPEALNRYFAFLDYKVKTGYDFDAKHT
                     EAEQRRVEDTFQVISLSFSNIGGPDVIGSYFATNGHRAYEDRVYRYLAEFYLVKLRYQ
                     DAATIYKSFVAQYPFHQASPHFSMRVVEIYDKGGVPQLVLTAKKDFAGTYGLQSEYWH
                     HFDVNKSPEVLNYLKSNLHDLANYYHAQYQDPKQHEQQAANYAEATRWYRESLSSFHT
                     DSQAPQTNYQLADLMLENHDYAAAAHEYESTAYDYPPHAKSAAAGYAAIYAYREYLKV
                     ASAEVKDAARRDTIASSIKFADTFPQHEQAAVVLAAASQDAYEMKDLVLARDSSRHLI
                     EKFPNATAGVRRDAWLVVGHSTFGLAEYADAEQAYGHVLEATPEADAAHAGLVENLAA
                     SIYKQGEQANQAGDYRTAANHFVRVKQVAPTSKICAAAEYDAGAALLHLKDWSAAAQV
                     LDEFRRSFPANDLQKEATKQIAYAYQQAGELAQSAGEYERVAKESPNPQLRAEALLQA
                     GDLYRQANDTDRALETYSRYVEQFPKPIETAVETRSKIAEICKSKGDQAQYT"
BASE COUNT         1284 a         2092 c         2172 g         1057 t
ORIGIN      
        1 gatcgacact tgcctggcgc aagagctcgg ggccgcctgg cgcgagtccg gtgtcgacca
       61 cggtgcgccg ctcgatgagc gcagcgcctg cgcactcgcc ctgggactct ccgcctgagg
      121 cggcattgcc caggcagcgg ctgattcaga aagcttttag gcggaattca agacggcgcc
      181 ggaccggtgc gccatcatca accccgtcgt agaagctaaa cttcgctaac caaccaggag
      241 acagccatgc ctaagttcgt gatcgagcgc gatattccag gtgccggcaa gctcaccccg
      301 agcgagctga agagcatctc gcagaagtcc tgcggggttc tctcgagcct cggcccgagc
      361 gtgcaatgga tacacagcta cgttaccgac gacaagatct actgcgtgta tcacgccaag
      421 gacgaatcgc tggtccgacg acacgcggag ctcggtggct ttccggcgaa tcgcatcgca
      481 cgcgtcatga cgatcatcga tcccggcact tcggaatgag gggtcgttgc cgcggcagct
      541 cgaggtcgtc ttcggagaaa tgccatggac gccactacat tgcgatcgat acaggctccg
      601 atcaaggagc gatacaaggg cgacgcgcaa gcggcgcaga ttactctgcg ggcaaagggc
      661 acgctcgatg ggtcgcgcat cgcctgcaaa gtggagaccg gccgcgcgct cgcggtggcc
      721 ggccttcatc cggctacggg cggctcgggg tcagagttgt gctcagggga catgttgctc
      781 gaagcgctcg ttgcctgtgc cggcgtgacg ctcaaggcag tggcgacggc actcgagatt
      841 ccggtgagat ccgccacggt gtgcgcggag ggagatctcg attttcgagg aacgctggga
      901 gtcgccaagg acgcccgggt cggtttcggc gcgatacggc tggcatttag cgtcgactcc
      961 gatgcgcccc aggagaagct cgaccagttg atgaagctta ccgagcgcta ctgcgtcgtc
     1021 tatcagacga tccgcggcgg ggtgccggtg gacgtgcgca tgagcagagg tgcttgaaat
     1081 ctccgccgat ttgccgttgt cttcgcgagt tatctgatct cgcatggccg ctccaggcgg
     1141 gccagagcac gtccttcctg aaccgcacct gaaacagacg taaagtaaag taacacgccg
     1201 ttgccgatta ccgtgtgtta cgaataagag cgtccgatgg aaacatcggg cggtgctagt
     1261 ggattcgcaa agggcaaacc caccgaaagg tggtgacgca aagtctccgg tctaaggagc
     1321 ctcggctcta cgacagcggg accgccgtaa tccagtccct ccaccggact gcatcggcgc
     1381 gtgttgttcg tgcatcacgg caacaggtgg gggagggcag gtcatgctca cttacgattc
     1441 gaacgccgta agcccacacg cttgggcgca tcccgaggcg gccggcgcag cgcgccttca
     1501 tcctgggcgg cccgcaacgg cctccgtccc ccgggagagg taagcgcgcc gctcggctgc
     1561 tgagcgcgcc gatccagcct tgccattcat gccacctgag tcatcaggtt ggaatcaact
     1621 ctggaacgat agagcaagag ccaatgcgta agcatttctt ggtcgtgatg agcgctctgc
     1681 tgtgcatttg cgcacggccg agtccggcgg acgagaacgc ccgcgcgcag atgaaaggtc
     1741 tggacgagca ggtccaggag gtcaaatccg acgtcctgag catcgccgcg gagctcaacc
     1801 agctcgaaga gaagctgctc tacccgtccg acactcacct cgccgtgttc gtgtcgatgc
     1861 cacacggcga gacgctgcgt ctcgatgcgg tgcagattca catcgacggc cagctggcga
     1921 ctcactacat ctacagcttc aaggagctcg acgccttgcg tcgcggcggc gtgcagcgga
     1981 tctacagcgg caacattgcc accggggcgc acgagatcga ggtcgccgtg agcggcaagc
     2041 tcgcgaacgg caaggactac agcgagaccg agcgcttccc gtttaccaaa gagatcaagc
     2101 cgaaactgat cggtgtgacg ttcgcaggcc ccgacggacc gcagcccggc atccacctcg
     2161 gggactggta gccgatgcgt cgtggcaagc agtggctgtc gatgggactg gcgctggccg
     2221 cggttgcggc gcggccgtct ccggctgccg atctgcgcga cctttatttc ggcgaggcgc
     2281 tctaccaggc gtaccaggga cagtatttcg acgctctcga gcggctcgac gccgagctcg
     2341 cccagcacca tcgcgtcgat gagccgcagc tggattcgct tcagtatcac atccggcagg
     2401 cggacttttc cgtcggtgac ttcgagctcc gttatcgcat gcatctgcgt gccgggcgtg
     2461 cgatcaaggc ggtgctcgat gctgacgtcg agcagcccgt caagaatgag gcagcgtacc
     2521 gactggcgcg catcgagttt cagaaggatc agcccgagga tgcgctgcag gccctggagc
     2581 ggattcaggg gacggtcccg gacgacatca aggatgacat cgagttcctg cgggccaacg
     2641 tgtacctcgc gctcgggcgg ccggccgacg ccgtggaggt gctcaagcgg gtgcagagtg
     2701 cgcagacctt gaagggattt gccgcctaca acctcggcat cgccttgctg caggcgggtc
     2761 atagcccgga gggcctgcgg cagctggata aggccggtca gatcgagagt catgaatcgg
     2821 ccacctcagc gatccgtgac aaatcgaaca tggtcctcgg cagcgtcatg ctgcgctcgg
     2881 aggattatgc gcacgctcaa ctcgccttcg atcgcgtgcg cctggaagga ccgtattcca
     2941 atcgtgcgct gctgagcacc ggctgggccg aagcttcggc gcatgactat cggaaggcgc
     3001 tggtaccgtg ggggatgctc atccggcgcg acccaaccga cgccgcggtg caggaggcga
     3061 gactcgcttt gccgttcgcc tacagcaagc tacaggttta cgggcgtgcg gcactccttt
     3121 atggccaggc gctcgattcc ttcggcaagg agctggagaa ggtggatgcc tccacgcgca
     3181 gcatccgcgc gggcaacttt ctcaaggccc tggtgcgcga ggaaatcaag caggacaacg
     3241 tgtgggtcgt gcgcctgcgc acgctgcctg acacgccgga gaccttctac ctgaccgagc
     3301 tgatggcctc gaacgacttc cagacggcgc tgcaaaacta cttggacatg gaggatctgc
     3361 gccgcaggct catggcctgg cagggcggtt tcgacgcatt cgcggatctc attcggctgc
     3421 gccgggcgaa ctacgagccg ctgctgccgc aggtggatgc gcatttcaga gagctggacg
     3481 cgcagatgcg cgtgcgcctc gagcagcgcg aacacctgca gaaacgtctg caggacattc
     3541 tgacggcgcc gcggccggta ctgctggcaa cggccgacga acgcgtggcg ctcgagcggc
     3601 tgcggaagat cgagacggcg ctgaaacatg cgagcggtcc ggaatgcgcc gcgcagcagg
     3661 tccggctgca gcacctccag ggcgtactca cgtggcgcct gtacacgcag tatccggagc
     3721 ggctgacgca ggcgcacaag gatctgcacg atctgaacga agacgtgcgc gtgcttcagg
     3781 aacgctacac cgcctacgta cgggcgcgtc aggcggcggt gcacagctat gccggctacg
     3841 acgagtcgat ccagcgcttg cgcacgcgtg tcggtgaggc gctggagcgc gtcaacacgc
     3901 tgttggcccg gcagggtcac gtgatcgaag cggtcgcgat cgacgagctc aacacccggc
     3961 gtcagcgcct cgaggcctat caggttcagg ctcgctacgc ggtcgccgac agctatgaca
     4021 gggcgctgaa ggaacatgcg actggaggca cgccatgatg cgtctcgtgc gtacgcgcgc
     4081 cgtcgcgttc ctcctgccgg tgattgcggc atgcgctcac caggccgacc gctcggggac
     4141 tctggcgacc ctccaccagg tgcgtccgga cctggaggag gttccggtcg accagggact
     4201 caaccgggcg gtgcagagct atggcgactt tctgaagcag gctccggaca gcgcgttggc
     4261 accggaggcc atgcggcgcc tggcggacct gaagatcgag aaggagttcg gactccaggg
     4321 cgacggcaag ctcgtggagc tatccgcctc ggaaagtgct gtgcaaggca aggccgttgc
     4381 gccgggattg ggcgccgcgc cagccacacc ggcgggcagc ctcgggcgcg acccggctgg
     4441 cgcattgcgc gcgccggcgg tcaccaggat cgacgcgcgc agcgctacac gctcgcacgc
     4501 cgcggcgccc ggcgccgggc cggcagcggc atcggaacgt gatctcgagc agcgagccgc
     4561 ccagttaggc atcccggccg cggacgcaat gacacccttg gcactgccag ccggaatgga
     4621 cggcgatgct gcgcgcgcgg gcccgctcga ggcgatcaag ctgtacgacg agctgctcgc
     4681 gaagtatccg cactacgcgc tgcgtgacca ggtgctctac cagaaggcgc gcgcctacga
     4741 ggagctgggc cagaccgagg acgccatgaa cgtcatggag cagctggtcc aggagaatcc
     4801 gcgctcgcgg ttctcggacg aggtgcagtt ccggcgcgcg gagcgcttct tcatcgccag
     4861 gaaatatcgt gaggcggaga gcgcgtacac ggcgatcgtc gatggcggca gcggctcgga
     4921 gtactacgag cccgcgctct acaaactggg atggacgctc tacaagcagc aactctaccc
     4981 ggaggccctg aaccgctact tcgcgttcct cgactacaag gtcaagaccg gctacgactt
     5041 cgatgccaag cacaccgagg ccgagcagcg gcgcgtcgag gacacctttc aggtcatcag
     5101 cctcagcttc tcgaacatcg gcggcccgga cgtgatcggc tcctacttcg ccaccaacgg
     5161 acatcgagcc tacgaggatc gcgtctaccg ctacctcgcg gagttctacc tggtgaagct
     5221 gcgctaccag gacgccgcca cgatctacaa atcattcgtg gcgcagtacc cgttccacca
     5281 ggcgtcgccg cacttcagca tgcgggtggt cgagatctac gacaagggcg gagtcccgca
     5341 gctggtgctc accgccaaga aggacttcgc cggcacctac ggcctgcaga gcgagtactg
     5401 gcaccacttc gacgtcaaca agtcgccgga agtgctgaat tacctgaaga gcaacttgca
     5461 tgacctggcg aattactacc acgcccagta ccaggatccg aagcagcacg agcagcaggc
     5521 ggccaactac gccgaagcca cgcgttggta ccgcgagtct ctcagctcgt tccacaccga
     5581 cagccaggcg ccgcaaacga actaccagct cgcggatctc atgctcgaga atcacgatta
     5641 cgcggcggcg gcgcacgagt acgagagcac cgcctatgat tacccgccgc acgccaagtc
     5701 ggcggcggcg ggctacgcgg cgatctacgc atatcgcgag tatctcaaag tcgcgagcgc
     5761 cgaagtgaaa gacgcggccc gccgcgacac gatcgcgagc tcgatcaagt tcgcggacac
     5821 cttcccgcag cacgagcagg cggcggtcgt gctggccgca gcatcacaag acgcgtacga
     5881 gatgaaggac ctggtgctgg cgcgcgactc cagccggcat cttatcgaga agttcccgaa
     5941 tgcgaccgcg ggcgtgcgcc gcgatgcgtg gctggtcgtc ggacactcca ccttcggcct
     6001 cgcggagtat gcggacgcgg agcaggccta cgggcacgtg ctcgaagcca ctccggaggc
     6061 tgatgcagcg cacgcgggac tcgtggagaa cctcgcggcc tccatctaca agcagggcga
     6121 gcaggcgaac caggcgggag attaccgcac tgctgccaac cacttcgtgc gagtcaagca
     6181 ggtagctccg acgtccaaga tctgcgccgc cgcggagtac gacgccggcg cggcgctcct
     6241 gcacttgaag gactggagcg ccgcggcaca ggtgctggat gagttccggc gcagcttccc
     6301 tgcgaacgac ctgcagaagg aagcgacgaa gcagattgcg tacgcgtacc aacaggcggg
     6361 ggagctggcg caatccgccg gtgagtacga gcgggtcgcg aaggagtcgc cgaatccgca
     6421 gctgcgcgcc gaagcgctgc tgcaggcggg tgacctctat cggcaggcca acgacaccga
     6481 tcgagcgctg gagacctact cccgctacgt cgagcagttc cccaagccga tcgagacggc
     6541 ggtggagacg cgctccaaga tcgccgagat ctgcaagtcg aagggagacc aggcccagta
     6601 caccc
//
LOCUS       DATDMP010000046         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1007828, whole genome shotgun sequence.
ACCESSION   DATDMP010000046 DATDMP010000000
VERSION     DATDMP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007828"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1985
                     /locus_tag="VI195_00830"
     CDS             <1..1985
                     /locus_tag="VI195_00830"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY6822955.1"
                     /translation="QGFSSDINTPGGPMHLTLAFNPSHLEIVNPVVEGSVRARQHRRK
                     DRGGDQVLPVLIHGDAAVAGQGVVMETLNLAQTRGYGTGGTVHIVINNQIGFTTSDPR
                     DSRSTLYCTDVMKMIEAPIFHVNADDPEAAVMAIEIAMDYRHQFKKDVVVDLICFRKL
                     GHNEQDEPMVTQPLMYKIVNAHPGTRKLYADRLAAQGVIATDEADKLVATFRQAMDGG
                     YHTNKTILANFKPPFAVDWSKYKGTKWNENDDTTLPLADLQMLAERLTAIPPNFKLHP
                     RVEKIIADRRLMGQGKLPVDWGMAENLAYASLLKDGYAVRISGQDAGRGTFFHRHAVL
                     HDQNREKWDAGSYIPLAHIAPNQGDFVVIDSVLSEEAVLGFEYGYSTSEPNELVVWEA
                     QFGDFVNGAQVVIDQFIAAGEVKWGRICGMVMMLPHGYEGQGPEHSSGRPERFLQLCA
                     DYNIQVCIPATPVQMFFLLRRQMIRPWRKPLVIFTPKSLLRHKESVSPLPEFAESKFR
                     AVNGEWDESLRPDKVKRLIVCSGKVFFDLMNARKEKGLKDTAIVRIAQLYPFPHDEFQ
                     AQIDLYAGASEVVWCQEEPGNQGAWHRIQHYILRHMRADQVLGYAMRASSASPAAGYL
                     QLHNEQQKALVEAAFAPLTDNLVLPVQGLGRKRRKK"
     gene            1985..>2496
                     /locus_tag="VI195_00835"
     CDS             1985..>2496
                     /locus_tag="VI195_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY6822956.1"
                     /translation="MLIEVKVPQLSESVAEATLLAWHKKQGEAVKRDENLIDIETDKV
                     VLELPAPADGVLVKIIKQAKESVGSGDVIAQIDTEAKAGAAAPAPSAPAAKPAAAPAP
                     AKSQPAVMPSAQKLAAEKGVDASKVQGSGRDGRVTKGDVLQQGSASAPAAKAPLPQAA
                     PPVNVDQLLAG"
BASE COUNT          469 a          845 c          833 g          349 t
ORIGIN      
        1 accagggatt ctcttcggac atcaacacgc cgggcgggcc gatgcacctg acgctcgcct
       61 tcaacccgtc gcacctggag atcgtcaacc cggtggtcga gggctcggtg cgggcgcgcc
      121 agcaccggcg caaggaccgt ggcggcgacc aggtgctgcc cgtcctgatc cacggcgatg
      181 cggcagtcgc cggccagggc gtggtgatgg agacgctcaa cctcgcgcag acgcgcggct
      241 acggcaccgg cggcacggtg cacatcgtga tcaacaacca gatcggcttc accacttcgg
      301 acccgcgcga cagccggagc accctgtatt gcaccgacgt gatgaagatg atcgaggcgc
      361 cgatcttcca cgtcaacgcg gacgacccgg aggcggcggt gatggcgatc gagatcgcca
      421 tggactaccg gcaccagttc aagaaggacg tggtggtgga cctcatctgc ttccgcaagc
      481 tcggccacaa cgagcaggac gagccgatgg tgacgcagcc gctgatgtac aagatcgtca
      541 acgcgcaccc cggcacgcgc aagctgtacg cggaccgcct ggcggcgcag ggcgtgatcg
      601 ccacggacga ggccgacaag ctggtggcca ccttccgcca ggcgatggac ggcggctacc
      661 acaccaacaa gaccatcctc gccaacttca agccgccctt cgcggtcgac tggtcgaagt
      721 acaagggcac gaagtggaac gagaacgacg acaccacgct gccgctggcc gacctgcaga
      781 tgctcgcgga gcggctcacc gcgatcccgc ccaatttcaa gctgcacccg cgcgtcgaga
      841 agatcatcgc cgaccgccgg ctcatggggc agggcaagct gccggtcgac tggggcatgg
      901 ccgagaacct cgcctacgcc tcgctgctga aggacggcta cgcggtgcgc atctccgggc
      961 aggacgccgg gcgcggcacg ttcttccacc gccatgcggt gctgcacgac cagaaccgcg
     1021 agaagtggga cgcgggcagc tatatcccgc tcgcgcacat cgcgcccaac cagggcgact
     1081 tcgtcgtcat cgactcggtg ctgtccgagg aggcggtcct cggcttcgag tacggctatt
     1141 cgacctccga gccgaacgag ctggtggtct gggaggcgca gttcggcgac ttcgtcaacg
     1201 gcgcgcaggt ggtcatcgac cagttcatcg ccgcgggcga agtgaaatgg ggccgcatct
     1261 gcggcatggt gatgatgctg ccgcacggct acgaggggca gggccccgag cactcttccg
     1321 gccgcccgga gcgcttcctg cagctgtgcg ccgactacaa catccaggtc tgcatcccgg
     1381 ccacgccggt gcagatgttc ttcctgctgc gccggcagat gatccgcccg tggcgcaagc
     1441 cgctggtgat cttcacgccc aagagcctgc tgcggcacaa ggagtcggtg tcgccgctgc
     1501 ccgagttcgc cgagagcaag ttccgggcgg tcaacggcga gtgggacgag agcctcaggc
     1561 ccgacaaggt gaagcggctg atcgtgtgca gcggcaaggt gttcttcgac ctgatgaacg
     1621 cgcgcaagga gaagggcctc aaggacacgg ccatcgtgcg catcgcgcag ctctacccgt
     1681 tcccgcacga cgaattccag gcgcagatcg acctgtacgc cggtgcgagc gaggtcgtct
     1741 ggtgccagga agagccgggc aaccagggcg cgtggcaccg catccagcac tacatcctgc
     1801 gccacatgcg cgcggaccag gtgctcggct acgcgatgcg cgcctcctcg gcctcgccgg
     1861 cggcaggcta cctgcagctg cacaacgagc agcagaaggc gctggtcgaa gccgcgttcg
     1921 cgccgctcac ggacaacctc gtgctgccgg tccagggcct gggccggaag agaaggaaga
     1981 agtaatgctg atcgaggtca aggtcccgca gctctccgag tcggtcgccg aggcgacgct
     2041 gctcgcctgg cacaagaagc agggcgaggc ggtcaagcgc gacgagaacc tgatcgacat
     2101 cgagaccgac aaggtcgtgc tcgagctgcc ggcgcccgcc gacggcgtcc tcgtcaagat
     2161 catcaagcag gcgaaggagt cggtcggctc cggcgacgtc atcgcccaga tcgatactga
     2221 agcgaaagca ggcgccgccg cgcccgcgcc ttcagcgcct gccgcaaaac ctgctgctgc
     2281 tcctgcgccg gcgaaatcgc agccggcagt catgccctcg gcgcaaaagc tcgccgccga
     2341 aaagggggtc gacgcatcca aggtccaggg ctccgggcgc gacggtcgcg tgaccaaggg
     2401 cgatgtgctc cagcaaggct cggcgtctgc gccagccgcg aaagcaccac ttccccaggc
     2461 tgcgcccccg gtcaacgtgg accagctgct ggcagg
//
LOCUS       DATDMP010000047         3823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_563689, whole genome shotgun sequence.
ACCESSION   DATDMP010000047 DATDMP010000000
VERSION     DATDMP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563689"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VI195_00840"
     CDS             <1..804
                     /locus_tag="VI195_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY6822957.1"
                     /translation="GEELTKLPLDLLQRGRYQPRMDMRPETLSELADSIKAQGVVQPI
                     VVRPLEPPLNGESQRYEIIAGERRWRAAQMAGLSEIPAVIRHIPDEAAIAMALIENIQ
                     RENLNPLEEARALERLVSEFGLTHQETAQAVGRSRAAVSNLLRLLELTAEVCELLEKR
                     MLEMGHARALLALTERRQQTEVALLVARKGLSVRETEALVRRLQAPPAAATQTSDGER
                     DPNVARLEQELAEKLGARVAIQAGRGGRGKLVVSYNSLDELDGILAHIQ"
     gene            984..1388
                     /locus_tag="VI195_00845"
     CDS             984..1388
                     /locus_tag="VI195_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY6822958.1"
                     /translation="MSAIDLPRARRLAFGVVVSQAAVSVVAALCALGLGGRLAALSAL
                     AGGGIATAGSLAMAGVVFGGSGGAAQRALQRFYLGEGLKLAVVVALFVVVLKVMTVAP
                     LAMFAAFAASFLVYWIALLKALPAVGGATRGA"
     gene            1430..2287
                     /gene="atpB"
                     /locus_tag="VI195_00850"
     CDS             1430..2287
                     /gene="atpB"
                     /locus_tag="VI195_00850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY6822959.1"
                     /translation="MAAAEGGGSATDYIVHHETFLSNKVPHGIVDFTAINLDTVFFSV
                     LLAVLFGGAFYVAARRATTGIPGRFQLFIEVLVNFIDTQVKDTFHGSSKLIAPLALTI
                     FAWILLFNIMDLVPVDLLPAAAHKAGFEHLKVVPSTDLNATFAMSLSVFALIIFYSIR
                     MKGVLGFISELTLHPFSAKNPFLQALLVPVNFLLESVTLLARPVSLSLRLYGNLYAGE
                     MIFLLIAVLTLSRGLDALVSVGGWASILAQLLLGFVWSVFHVLIIVLQAFIFMVLTIV
                     YLALASEHH"
     gene            2352..2615
                     /gene="atpE"
                     /locus_tag="VI195_00855"
     CDS             2352..2615
                     /gene="atpE"
                     /locus_tag="VI195_00855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY6822960.1"
                     /translation="MDIAQVQAMTVLAVGIIFGLGAAGTAIGFGILGGRFIEGSARQP
                     ELLPVLRIQMFVVAGLLDAVAMIGIGFALFFTFANPFLGALHH"
     gene            2649..3119
                     /locus_tag="VI195_00860"
     CDS             2649..3119
                     /locus_tag="VI195_00860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY6822961.1"
                     /translation="MNINLTLIVQMIVFILLIWFTMRFVWPMILGPMNEREAKIAQGL
                     AAAEDGERTLAEARGKADAIIREARERANQIIDHAQHRANELIEQAKGAASSESARIV
                     AAAQQQIVLDTTRAKESLRREVAGIAVGAATKLLGREIDPRAHADLLEQLAEQI"
     gene            3130..3666
                     /locus_tag="VI195_00865"
     CDS             3130..3666
                     /locus_tag="VI195_00865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY6822962.1"
                     /translation="MADRLTIARPYARAAFEEARKNGGLAPWSTALQTAAIVVQDARV
                     ASLLDNPRVTPAELARLVDEIAATPLGEHGANFVRTLADNHRLGLLPEIAELFDTLKD
                     EAEGIADVTVTSAAPLDEAQRARIGAALERRLARKARLHFEIDPALIGGAVLRAGDLV
                     IDGSLRTRLERIAYELTA"
BASE COUNT          628 a         1211 c         1304 g          680 t
ORIGIN      
        1 ggggaagagc tcaccaagct gccgctcgac ctgctgcagc ggggacgcta ccagccgcgc
       61 atggacatgc ggcccgagac gctcagcgag cttgccgact ccatcaaggc gcagggcgtc
      121 gtgcagccga tcgtggtgcg cccgctcgag cctcctctca acggcgaatc ccagcgctac
      181 gagatcatcg ccggggagcg ccgctggcgc gccgcgcaga tggccgggct cagcgagatt
      241 ccggccgtca tccgtcacat ccccgatgaa gcggccattg cgatggcgct catcgagaac
      301 atccagcgtg agaacctcaa tccactcgag gaggcccggg cgctcgagcg cctggtgagc
      361 gagttcggac tgacccacca ggagacggcg caggcggtgg gacgctcgcg cgctgcggtc
      421 agcaatctgc tgcggctgct ggagctcacg gccgaggtgt gcgagctgct cgagaaacgc
      481 atgctggaga tggggcacgc gcgcgcgctg ctcgcgctca ccgaacggcg acagcagacc
      541 gaggtcgcgc tgctcgtcgc ccgcaagggt ctttccgtgc gcgagaccga ggcactggtg
      601 cggcgtctgc aggctccgcc cgccgcggcc acgcaaacga gtgacggcga gcgcgatccg
      661 aatgtggcac ggctcgagca ggaactcgcg gagaagctcg gagcacgcgt tgcgatccag
      721 gcgggcagag gcggccgcgg caagctggtg gtgagctaca acagcctgga cgagctcgac
      781 ggcatcctcg ctcacattca gtagcgcgcc gcggtgcgga aagtacgatg tggacgggcg
      841 cgcaggcgct ctctataatc ccgcggcttt ttgccatggt cacgtaagtg actgaatggc
      901 cgaagtaatg tgaagccact tgcgttcgct caagacgcaa ggcaagcggc tcttttgagc
      961 ccctcgaacg ggacttatat aaggtgtcgg cgatcgatct gccccgcgcg cggcggctgg
     1021 ccttcggcgt cgttgtgagt caggcggcgg tgagcgtcgt ggcggcgctg tgcgccctag
     1081 gcctcggcgg ccgcctggcg gcactctccg cgctggcggg cgggggtatc gccacagcgg
     1141 gaagtctcgc gatggcgggt gtggtgttcg gcggctcggg cggcgcagcg cagcgcgcct
     1201 tgcagaggtt ctacctcggc gaggggctga agctcgccgt tgtagtcgcg ctgttcgtgg
     1261 tggtgctgaa ggtgatgaca gtggcgccgc tggcgatgtt cgccgcgttc gccgcgagct
     1321 tcttggtgta ctggatcgcg ttgctgaagg cgctgccggc cgtgggcggc gcgacccgcg
     1381 gcgcgtgact ggcggtggag tcatcgtgag tggatcgttc acgggtttgg tggcggcggc
     1441 ggaaggtggc ggcagcgcca ccgactacat cgtgcaccac gagacgttcc tgtcgaacaa
     1501 ggtgccgcac ggcatcgtcg acttcacggc catcaatctc gataccgtgt ttttctcggt
     1561 actgctcgcg gtgctgttcg gcggcgcgtt ctatgtcgcg gcccgccgcg cgaccacggg
     1621 tattcccggc aggttccagc tgttcatcga ggtgctggtc aatttcatcg acacgcaggt
     1681 gaaggacacc tttcacggca gcagcaagct gatcgcgccg ctggccctga ccatttttgc
     1741 ctggatcctg ctgttcaaca tcatggacct cgtgccggtg gacctgctgc cggcggcggc
     1801 ccacaaggcg ggattcgagc atctgaaggt cgtgccgagc accgacctca acgcgacctt
     1861 cgcgatgtcg ctgagcgtgt tcgcgctgat cattttctac agcatcagga tgaaaggtgt
     1921 gctcgggttc atcagcgagc tgacgctgca ccccttcagc gccaagaatc ctttcctgca
     1981 ggcactgctg gtgccggtca atttcctgct cgagtcggtc accttgctgg cgcgcccggt
     2041 atcgctgtcg ctgcgtctct acggcaacct gtacgcgggg gagatgatct tcctgctgat
     2101 cgcggtactc acgctgtcgc gcgggctcga tgcgctcgtg agcgtcggcg gctgggcgtc
     2161 gatcctcgca cagctgctgc tcggattcgt atggtcggtg tttcacgtac tgatcatcgt
     2221 gctgcaggcc ttcatattca tggtgctgac catcgtctac ctggcgctgg ccagtgagca
     2281 ccattgagga gcaggcgcga gttactggtt tcccgtttcc ggtatccgtt gcgtatcttg
     2341 aggagagttg aatggacatc gcacaagtac aggccatgac cgtgttggcc gtcggcatca
     2401 tcttcggcct gggcgccgcc ggcactgcca tcggtttcgg catactcggc gggcgcttca
     2461 tcgagggctc ggcccgtcag ccggagctgc tgcccgtgct gcgaatccag atgttcgtgg
     2521 tcgcgggcct gctggacgcg gtcgcgatga tcggcatcgg cttcgcgctg ttcttcacgt
     2581 tcgcgaaccc gtttctcggt gccctgcacc attgagcgct tggcgagcgg gcggcgggag
     2641 atagcacggt gaacatcaat ctcaccctga tcgtgcagat gatcgtgttc atactgctga
     2701 tctggttcac gatgagattc gtctggccca tgattctcgg cccgatgaac gagcgcgaag
     2761 ccaagatcgc gcagggcctc gccgccgccg aggacggcga gcgcacgctc gccgaggcgc
     2821 gcggcaaggc cgatgccatc atccgcgagg cgcgcgaacg cgccaaccag atcatcgacc
     2881 atgcgcagca ccgcgccaac gagctgatcg agcaggccaa gggcgcagcg agctcggaga
     2941 gcgcgcgcat cgtcgccgcc gcgcagcagc agatcgtcct cgacaccaca cgcgccaagg
     3001 agagtctgcg gcgcgaggtg gcgggcattg ccgtgggtgc cgctaccaag ttgctcgggc
     3061 gcgagatcga cccgcgcgcc cacgccgatc tgctcgagca gctcgcggag cagatctgag
     3121 cgcgtagcga tggcagatcg actcaccatc gcccgaccgt acgcgcgcgc cgccttcgag
     3181 gaggcgcgca agaacggcgg cctcgcgccc tggtcgactg cgctgcagac cgcggccatc
     3241 gtcgtgcagg acgcgcgtgt agcgagcctg ctcgacaatc cgcgcgtgac gccggccgag
     3301 ctggcgcggc tcgtggatga gatcgcggcc actccgctcg gcgagcacgg cgcgaacttc
     3361 gtgcgtacgc tcgccgacaa ccaccggctg gggcttctgc cggagatcgc cgagctgttc
     3421 gatacgctga aggacgaggc cgaaggtatc gccgacgtca cggtgacctc ggcagcgcca
     3481 ctcgatgaag cgcagcgcgc caggatcggc gcggcactcg agcggcggct cgcgcgcaag
     3541 gcccgcctgc acttcgagat cgacccggcg ctcatcggcg gtgcggtgct gcgcgccggt
     3601 gacctggtga tcgatggctc gttgcgcacg cggctcgagc gcatcgccta cgaactgacc
     3661 gcttaacagc acccatgaaa ttgagcttcg acatgcgttg caagcagacg gccgtccgcc
     3721 aagacgctca atttcatggg gcccgcttgg gaaagatctt cagaaggaag taatggtatg
     3781 gcgatcaagg caaccgagat cagcgatctc atcaagcaac gca
//
LOCUS       DATDMP010000048         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2007807, whole genome shotgun sequence.
ACCESSION   DATDMP010000048 DATDMP010000000
VERSION     DATDMP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007807"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VI195_00870"
     CDS             <1..433
                     /locus_tag="VI195_00870"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY6822963.1"
                     /translation="AALIAGFAAQWSAADVGVKVLYPASSRALPTIATGLRQLGASVL
                     QVEAYRTQAAALDIDECRAWIARGGIGAVTFSSPSAVSELAHALGKADFQRLLAEAAA
                     VAIGRTTAAEVNAHGHDAVVAESATLHGLAVTTLRLLQTRA"
     gene            454..1416
                     /gene="hemB"
                     /locus_tag="VI195_00875"
     CDS             454..1416
                     /gene="hemB"
                     /locus_tag="VI195_00875"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY6822964.1"
                     /translation="MRPRRTRAHDAWRRLVRETRLTADALIYPLFVVPGRAVRHPVTS
                     MPGIAQLSVDETVKEARAAEAAGVPGVLLFAAPERKDARASDALDARGLAASAIAAIK
                     EACPRLLVWADVCLCGATDHGHCGHVSQDGTIDNDSSIETLADVALNYARAGADAVAP
                     SDMMDGRVAAMRARLDAHGHAHTPIVSYAAKYASSFYGPFREAADSAPAFGDRRSYQM
                     DPANAREALREVLLDVEEGADLVMVKPAGPYLDIIRQVRDAVHVPVVAYQVSGEFSLI
                     KAAAERGWIEERAAVLETLTGIRRAGADLIISYFAPQVAQWLRT"
     gene            1413..>2011
                     /locus_tag="VI195_00880"
     CDS             1413..>2011
                     /locus_tag="VI195_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY6822965.1"
                     /translation="MTAPAPALESSFLAACRREPVRHRPIWIMRQAGRYLPEYRALRA
                     KVDFETLTRTPALAAEVTLQPLRRFPLDAAILFSDIMTPLQGMGVGLSFEPAPVVREP
                     IRTLAQVEALPALAPARDVPFVLDSIRLVRAGLPRGVPLIGFAGAPFTLMCYLVCGRP
                     SKEFAAARAFLYAQAGTAERLLGRLADAMAEYLAAQAAAG"
BASE COUNT          290 a          682 c          697 g          342 t
ORIGIN      
        1 ggccgcgctg atcgcaggat tcgccgcgca atggtcggcc gctgatgtcg gggtgaaggt
       61 gctttacccg gcaagctcgc gcgctttgcc cacgatcgct accggactgc ggcagctcgg
      121 cgcatcggtc ctgcaggtcg aagcctatcg tacgcaggcc gccgcgctcg acatcgatga
      181 atgccgcgcc tggatcgctc gcggtggaat tggcgcagtc accttctcca gtccttccgc
      241 ggtaagcgag ctcgcacacg cgctgggcaa ggccgacttc caacgcctgc tcgcggaggc
      301 cgcggcggtc gcgatcggcc gcaccactgc cgccgaggtc aatgcacatg gtcacgatgc
      361 cgtggtcgcg gagtccgcga cgctgcacgg gctggcggtc accacacttc gcttgttgca
      421 aacgagagcc tgaagactat gggatttccg caagtgagac cgcgacgtac ccgcgcgcac
      481 gatgcgtggc ggcgtctggt gcgtgagaca cgcctgacgg cggatgcgct catctatccg
      541 ttgttcgtcg taccggggcg tgcggtgcgc cacccggtca cgagcatgcc ggggatcgcg
      601 cagctatcgg tagatgagac ggtgaaggaa gcgcgcgcgg ccgaggcggc cggggtgccg
      661 ggggtgctgc tgttcgccgc gcccgagcgc aaggatgcac gcgcgagcga tgccctcgat
      721 gcgcgtggcc tggcggcgag cgccatcgcc gccatcaagg aagcgtgtcc gaggcttctg
      781 gtgtgggcgg atgtctgtct gtgcggagcc accgatcacg gtcactgcgg ccacgtgtcg
      841 caggacggca cgatcgacaa cgactcctcc atcgagacac tggcggatgt ggccctcaac
      901 tacgcgcgtg ccggagcaga tgcggtggca ccgagtgaca tgatggacgg gcgcgtcgcc
      961 gcgatgcgcg cgcgcctcga tgcgcacggg catgcccaca ctccgatcgt ctcttacgcc
     1021 gcgaaatacg cgtcgtcctt ctatggaccc ttccgcgagg cggcggactc tgcgcccgcc
     1081 ttcggcgacc gccgctcgta tcaaatggat cccgccaacg cgcgcgaagc gttgcgggag
     1141 gttctgctcg atgtggagga gggcgccgac ctcgtcatgg tgaagccggc aggcccgtac
     1201 ctcgacatca tccgccaggt acgcgacgcg gtacacgtgc cggtggtcgc ctaccaggtc
     1261 tcgggcgagt tcagcctcat caaggcagcc gccgagcgcg gctggatcga ggagcgggcg
     1321 gcagtgctcg agacgctcac gggtattcgc cgcgccggcg ccgatctcat catcagctac
     1381 tttgcgccgc aggtggcgca gtggctgcgg acatgactgc gcccgcgccg gcgctcgaga
     1441 gctcatttct tgccgcctgc cgccgtgagc ccgtgagaca tcggccgata tggatcatgc
     1501 gtcaggccgg ccgctacctg ccggaatacc gtgcgttgcg ggccaaggtg gatttcgaga
     1561 cgctgacgcg cacacccgcg ctcgccgccg aggtcactct gcagccgctg cgccgtttcc
     1621 cgctcgatgc cgccatcctg tttagcgaca tcatgactcc gctccagggc atgggtgtcg
     1681 gactctcgtt tgagcccgcg ccggtggtgc gcgagccgat ccgcaccctg gcgcaggtgg
     1741 aggcgctgcc ggcgctcgcg cccgcgcgcg atgtgccttt cgtgctcgac agcatccgtc
     1801 tcgtgcgggc tggcctgccg cgcggcgtgc cgctcatcgg ttttgccgga gcgccgttca
     1861 cgctcatgtg ctacctggtc tgcggacggc cttcgaagga atttgccgcc gcgcgcgcct
     1921 tcctctatgc gcaggccggc accgcagagc ggctgctggg ccgcctcgcg gacgccatgg
     1981 cggaatatct cgcggcccag gctgcggccg g
//
LOCUS       DATDMP010000049         2977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1230519, whole genome shotgun sequence.
ACCESSION   DATDMP010000049 DATDMP010000000
VERSION     DATDMP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230519"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..659)
                     /locus_tag="VI195_00885"
     CDS             complement(18..659)
                     /locus_tag="VI195_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822966.1"
                     /translation="MRLLPLILFFAAAAAFGDEAADAKRRWQESPYGPLLERILPPTF
                     EASQLPEPASAGAKLVQRYCVQCHNLPNPAMHNAAKWRGIVERMVLRMEGRGNMGTLM
                     ADMMAGVKAPSPEEQRSLLAYLRRYSQKPLDPKRYPEVAQPSAEAFRLACSQCHVLPD
                     PRRHTAAEWRAVVARMQQNMQWMNRVVGSQPDADEPQLRVKDILAFLQRYARP"
     gene            complement(662..1711)
                     /locus_tag="VI195_00890"
     CDS             complement(662..1711)
                     /locus_tag="VI195_00890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6822967.1"
                     /translation="MLSRVVGEIAASGGWISFARYMELVLHEPGAGYYASGAQKFGAG
                     GDFVTAPELGSLYGRTLARQLAELRCERILEVGAGSGALAHTLLEQLDCEYLILETSP
                     DLAARQRERLGTSAKWLDALPRHFRGVIVANEVLDAMPVHALAWTADGIMERGVAFER
                     DHLVWCERPASGELLRQAQAIAVQPRYESEIGLVGQAWVGSIAESLEQGALFLIDYGF
                     AHSEYYHPQRAMGTLMCHHRHQSHGEVFFQPGRQDVTAHVDFSAIARAATAAGLEVLG
                     YATQAQFLVNCGITDVLAQANVENALRYAPLASEAQKLLSPAEMGELFKVLAVGRRLA
                     RPLPGFSHCDRLPTL"
     gene            1744..2490
                     /locus_tag="VI195_00895"
     CDS             1744..2490
                     /locus_tag="VI195_00895"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEY6822968.1"
                     /translation="MAWRLEGKAVLVTGGARRVGAAIARRLHAAGANVLLHYRDSEAD
                     AASLAAELNAAREKSAATVKAELLAPIAPKALVAAALEHFGRLDVLVNNASSFFPIAV
                     GAIEASHWEELIGSNLRAPLFIAQEAAPQLARQLGSIVNIVDIHADRPLKGYPVYSIA
                     KAGLAALTRSLALELAPEVRVNGVAPGAIAWPEDGQFPPEERARVLATTPLRRIGEPD
                     DIAQAVHFLACAPYVTGQIIAVDGGRSVFL"
     gene            2553..>2977
                     /locus_tag="VI195_00900"
     CDS             2553..>2977
                     /locus_tag="VI195_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6822969.1"
                     /translation="MGEAIADFSMIEEGDRVMVCLSGGKDSHALLDVLLSLQEKSPVR
                     FALIAVNLDQKQPGFPEHVLPQYLEKLGVAYRIVEQDTYSVVKRVIPEGKTMCGLCSR
                     LRRGVLYRVATELGATKIALGHHRDDILETFFLNLFFGG"
BASE COUNT          485 a         1007 c          997 g          488 t
ORIGIN      
        1 cagcggcacg ccgcgaatca tggccgcgcg tatctttgca ggaaggcgag aatgtctttc
       61 accctgagct gcggctcgtc cgcgtcgggc tgcgagccga ccacgcggtt catccactgc
      121 atgttctgct gcatgcgcgc caccacggca cgccactcgg cggcggtatg gcggcgcgga
      181 tccggcagca cgtggcactg gctgcacgcc aggcgaaaag cctccgccga aggctgcgcc
      241 acctcgggat agcgcttcgg gtcgagcggt ttctgggaat accttctcag ataggcgagc
      301 aagctgcgct gctcctcggg cgaaggcgcc ttgacgccgg ccatcatgtc ggccatcagc
      361 gtgcccatgt tgccgcgtcc ctccatgcgc agcaccatgc gttcgacgat gccgcgccac
      421 ttggccgcat tgtgcatcgc cgggttgggc aggttgtggc actgcacgca gtagcgctgc
      481 acgagcttcg cgcccgccga ggccggctcg ggaagctggc tcgcctcgaa ggtgggcggc
      541 aggatgcgct cgagcagcgg gccgtacggg ctttcctgcc agcgcctctt cgcatcggcg
      601 gcttcgtcgc cgaacgccgc cgcggcggcg aagaaaagga tgagcggaag caagcgcatc
      661 gctagagcgt aggcagccga tcgcagtgcg agaagccggg aagaggccgc gcgagcctgc
      721 gcccgaccgc cagcaccttg aagagctcgc ccatctcggc cggcgacagc agcttctgcg
      781 cctccgaggc gagcggcgcg tagcgcagcg cattctcgac gttcgcttgc gcaagcacat
      841 cggtgatgcc gcaattcacg aggaattgcg cctgcgtcgc atagccgagg acctcgaggc
      901 cggcagccgt tgccgcgcgc gcaatggcgc tgaaatccac atgcgcagtc acgtcctgcc
      961 ggccgggctg gaagaacacc tcgccgtgcg actggtggcg gtgatggcac atgagcgtgc
     1021 ccatggcgcg ctgcggatgg tagtactcgc tgtgcgcgaa tccgtagtcg atgaggaaaa
     1081 gcgcgccttg ctcgagcgat tcggcaatgg agcccaccca agcctggcct accagcccga
     1141 tctcgctttc gtagcgcggc tgcacggcga tcgcctgcgc ttgccgcagc agttctccgg
     1201 aggcggggcg ctcgcaccac accaggtggt cgcgctcgaa cgccacgccc cgctccatga
     1261 ttccgtccgc agtccacgcg agcgcgtgga ccggcatcgc atccagcacc tcgttggcca
     1321 cgatgacgcc gcggaaatgg cgcggcaggg cgtcgagcca tttggcgctt gtgccgaggc
     1381 gctcgcgctg gcgcgcggcg agatccgggc tcgtctcgag gatgagatat tcgcagtcga
     1441 gctgctcgag aagcgtatgc gcgagcgcgc cgctgccggc accgacctcg aggatccgct
     1501 cgcagcgcag ctcggcaagc tggcgtgcga gggtgcggcc gtagagcgag ccgagctccg
     1561 gcgcggtgac gaagtcgccg ccggcgccga acttctgcgc gccgctcgcg taatagcccg
     1621 cgcccggctc gtgcagcacg agctccatgt accgggcgaa cgagatccag ccgccgcttg
     1681 cggcgatctc accgacgacg cgggaaagca tgtaagaaat ttaccctaga atcctctcgt
     1741 accatggcgt ggcggctcga aggcaaggcg gttctcgtga ctggcggcgc gcggcgcgtc
     1801 ggcgccgcca tcgcgcgccg gctgcatgcg gcgggcgcga atgtgctcct gcattaccgc
     1861 gattccgaag ccgacgccgc gagcctcgcg gccgagctga atgcggcacg cgaaaaaagc
     1921 gccgccaccg tcaaggcgga gcttctcgcc ccgattgcgc ccaaggcgct cgtagccgcg
     1981 gcgctcgagc acttcggccg cctcgatgtc ctcgtcaaca acgcttccag ctttttcccg
     2041 attgcagtgg gggccatcga agcttcgcac tgggaagagc tgatcggctc caacctgcgc
     2101 gccccgcttt tcatcgcgca ggaggccgcg ccgcagctcg cgcgccagct gggctcgatc
     2161 gtgaacatcg tggacatcca cgccgatcgg ccgctcaagg gttatccggt ttacagcatt
     2221 gccaaggcgg gcctcgctgc gctcactcgt tcgctcgcgc tggagcttgc gcccgaagtc
     2281 cgcgtcaatg gcgtggcgcc gggcgccatc gcatggcccg aggacgggca attccccccg
     2341 gaagagcgtg cgcgagtcct cgccacgact ccgctgcggc gcatcggcga gccggacgac
     2401 attgcccagg cggtacactt cctcgcctgc gcgccgtacg ttacgggcca gatcatcgcc
     2461 gtcgacggcg gccgttcggt gttcctgtga aagctgacct ccgcaagctc gaatacgaag
     2521 agcacaagct cgccaagcgg ctgcgccgcc aggtgggcga ggccatcgcc gatttctcga
     2581 tgatcgagga gggcgaccgc gtgatggtgt gcctctcggg cggcaaggac agccacgcgc
     2641 tcctcgatgt gctgttgtcg ctccaggaaa agtcgccggt gcgcttcgcg ctcatcgccg
     2701 tcaacctcga ccagaagcag ccggggttcc ccgagcacgt gctgccgcag tacctggaaa
     2761 agctcggcgt ggcctaccgc atcgtggagc aggacaccta ttcggtcgtg aagcgcgtga
     2821 ttcccgaggg caagacgatg tgcggcttgt gctcgcggct gcggcgcggc gttttatacc
     2881 gggtcgcgac cgaactgggc gcgacgaaga ttgcgctcgg ccaccaccgc gacgatatcc
     2941 tcgaaacgtt ttttctcaat ttgttttttg gcggcaa
//
LOCUS       DATDMP010000050         3030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1674883, whole genome shotgun sequence.
ACCESSION   DATDMP010000050 DATDMP010000000
VERSION     DATDMP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674883"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VI195_00905"
     CDS             <1..1150
                     /locus_tag="VI195_00905"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY6822970.1"
                     /translation="LVPSAGSVENLARLNDPRSKVAVGFAQGGLTSEAKSPDLESLGT
                     VFYQPFWLFVRRGISLARPEEIRGKRVAAGPDGSGTQVMAKKFLTLNGIDESTLQLVP
                     LPATEAGEVLLRGELDAAAMVASWDTPIVHRLLASPDINIVGAPRADAYVALDPYLTK
                     LVLPTGVGDLASNRPPNDVPLVATEASLIVRKDLHPAIQYLLLEAATEIHSAGGLFRK
                     AGQFPAPAPVDLPISKDARQFYKSGSPFLQRYLPYGIAVLASRLLLLLIPLIGVAYPL
                     LRTAPTLYGWSMRRRIFRLYGELKYIEAELGASGGVPAADLLAQLQRLEERANRLQLP
                     FAFAPFLYQLRNHIALVRDRILAVTSSSAPPSLQGSAAAAAIETSRAG"
     gene            1317..2837
                     /locus_tag="VI195_00910"
     CDS             1317..2837
                     /locus_tag="VI195_00910"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY6822971.1"
                     /translation="MGHTPDGASTDRRCTSGIEGLDQILAGGMPRDCFYLVQGDPGSG
                     KTTLALQFLLEGRRQGEAVFYVTLSETAAELEKVARSHGWTLADIPMLELSAIESLLR
                     PEGQTTVFHPSELELNNVTRLVLSETRKHQPRRIVFDSLSEFRLMAETPLRYRRQLLV
                     LKQEFARQRSTALLLDDKMDNNLIGSDPHVLSLTHGVIEMQQLSPDYGKSRRRLRVLK
                     MRGVKFREGYHDYVIDTGGIRLFPRLTASDHHNEFKRETVPSGVRELDDLLGGGLDAG
                     TTALILGHAGTGKSTLALQYACKLASQGGRSILFAFDETLGIVLERASKLGLDLRAHV
                     RDGLVTLQQLDPAEISPGEFAHRILASVQAGCKLVVIDTLNGYLNAMPGEKFLINQLH
                     ELSAYLNQQGVLTIFVLAQQGLAITAEAPVDLSYLADTVLSLRFFEMAGEVRNALSVI
                     KKRSGHHEHTIREFMLEADRGIRIGAPLREFRGVLTGVPEFVGTTGQMLEQSKNEH"
     gene            2827..>3030
                     /locus_tag="VI195_00915"
     CDS             2827..>3030
                     /locus_tag="VI195_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822972.1"
                     /translation="MSTEHAERVLVVAPLGGDAAAIAAVLTGSGYAARICEGAAEASV
                     QLAAGAGSLLLTEEALEREHVPQL"
BASE COUNT          531 a         1003 c          952 g          544 t
ORIGIN      
        1 cctcgtgccc tccgcaggct cggtggaaaa tctcgcccgg ctgaatgatc cgcgttcgaa
       61 agtcgcggtc ggattcgccc agggcggtct cacgagcgag gcgaaatcgc ccgatctcga
      121 gtccctcggt acggtgttct accaaccctt ctggctcttc gtccgacgcg ggatctcgct
      181 cgcgcggccc gaagagatac gcggcaagcg cgtggcggcc ggaccggatg gcagcggcac
      241 gcaggtcatg gcaaagaagt tcctcacact gaacggcatc gatgagagca cgttgcagtt
      301 agtgccgctg cccgcgaccg aggccgggga ggtcctgctg cgcggcgagc tcgacgcggc
      361 tgcgatggta gcgtcatggg atacccccat cgtgcatcgt ctgctcgcct cgccggacat
      421 caacattgtc ggagcacccc gcgcggacgc ctacgttgcg ctggacccgt acctcaccaa
      481 actggtgctg cccaccgggg tcggtgacct tgccagcaac cggccgccga acgacgtgcc
      541 gctcgtagct accgaagcca gcctgatagt ccgcaaggat ctgcacccgg ccatacagta
      601 cctactgctg gaggccgcaa cggagatcca ctctgcgggc ggcctctttc ggaaagctgg
      661 ccagttcccc gcgcccgcgc cggtcgacct gccgatctcc aaggacgcgc ggcagttcta
      721 caagtccggt tccccgtttc tgcagcgcta tctgccgtac ggtattgcgg tcctcgccag
      781 caggctgctg ctgcttctga tcccactcat cggtgtcgct taccctctgc tgcgcacggc
      841 tccgacgctt tacggctgga gcatgcggcg gcgcatcttt cgactgtacg gcgagctcaa
      901 gtacatcgaa gccgagctcg gtgcttcggg cggagttccc gccgcggacc tgttggcgca
      961 gctgcagcgg ctcgaggagc gcgcgaaccg cctgcagctg ccattcgcct tcgcgccgtt
     1021 cctctaccaa ttgcgcaatc acatcgccct ggtgcgcgac cgaatcctcg cggtcacttc
     1081 cagctctgcc ccgccatcgc tgcaaggatc tgcggcagcc gctgcgatcg aaacctcacg
     1141 cgcgggctga tatgcactgc gcagtgtcag cctgcgctga caggaagtcg cgttggcctc
     1201 cgacctgtct gcgcccgcgc tcacgcctat agtcaccgcg ccagcgggtt gccggtacgg
     1261 tgagcacgga gccgtccccg cgcccgtact ccacataact caaatggatc aaccctatgg
     1321 gtcacactcc cgacggcgcc tcgaccgaca gacggtgcac gagcggtatc gagggactcg
     1381 atcagatact cgccggcggt atgccgcgcg attgctttta tctcgtgcag ggagatccgg
     1441 gttccggcaa gaccactctg gccctgcagt tcctgctgga aggccggcgc cagggagaag
     1501 cggtcttcta cgtcaccctg tcggaaaccg ccgcggagct cgagaaggtc gcgcgctctc
     1561 acggctggac gcttgccgat atcccgatgc tcgagctctc ggcgatcgag tccctgctgc
     1621 gccccgaggg ccagaccacg gtgttccatc cgtcggagct cgagctcaac aacgtgacac
     1681 gactcgtgct gtcggagacg cgcaagcacc aaccgaggcg catcgtattc gactcattgt
     1741 ccgaatttcg cctgatggcg gaaacgccgt tgcgttaccg gcggcagctc ctggtgctga
     1801 agcaggagtt cgcccggcag cgcagcacgg cgctgctgct cgatgacaag atggacaata
     1861 acttgattgg ttcagatccg catgtcttga gcctgacgca cggcgtgatc gaaatgcagc
     1921 agctctctcc agattacggc aaatcgcgcc ggcgattacg cgtactgaag atgcgcggcg
     1981 tcaagttcag ggagggctac cacgattacg tcatcgacac aggcgggata cgcctgttcc
     2041 ctcggctcac cgcctccgac catcacaacg agttcaagcg cgagacggtg ccgagcggtg
     2101 ttcgggagct cgatgacttg ctcgggggcg gactggatgc gggcaccacg gcgctcatcc
     2161 tgggtcatgc cggcacggga aaatcgacgc tggcactgca gtacgcgtgc aaactcgcct
     2221 cccagggtgg ccgcagcata ctgttcgcgt tcgatgaaac cctcggcatt gtgctcgagc
     2281 gtgcctcgaa gctcggactc gatctgcgag ctcatgttcg cgacgggctc gtcaccttac
     2341 agcagctgga cccggcggag atctcacccg gcgagttcgc gcaccggata ctcgcgagtg
     2401 tgcaggcggg ctgcaagctc gtggtgatcg acacactcaa tggttacctc aacgccatgc
     2461 ccggggagaa gttcctcatc aatcagttgc acgagctctc ggcgtacttg aatcagcagg
     2521 gcgttctgac catcttcgtg ctcgcgcagc agggcctggc catcaccgcc gaagcacccg
     2581 tggatctcag ttacctggcc gacacggtgc tgagcctgcg attcttcgag atggccggcg
     2641 aggtgcgcaa cgcgctctcg gtcatcaaaa agcgcagtgg ccatcatgag cacacgattc
     2701 gcgagttcat gctcgaggct gaccgcggca tccgcatcgg agcgccgctg cgggagttcc
     2761 gcggcgtgct gaccggagtg ccggaatttg tggggacgac aggccagatg ctcgagcaat
     2821 cgaagaatga gcactgagca cgcggagcga gtcctcgtcg tcgccccgct ggggggcgac
     2881 gccgcagcca ttgccgcggt tctgaccggc agcggctatg ccgcgaggat ctgcgagggc
     2941 gcagcagagg cgagtgtgca gctcgccgcc ggtgccggat cgctgctgct caccgaggag
     3001 gcgctcgagc gcgagcacgt gccgcagctg
//
LOCUS       DATDMP010000051         4048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_230877, whole genome shotgun sequence.
ACCESSION   DATDMP010000051 DATDMP010000000
VERSION     DATDMP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230877"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="VI195_00920"
     CDS             <1..2324
                     /locus_tag="VI195_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6822973.1"
                     /translation="GTTWHAAGLMVTFGSTSETSTEMRKYARALYSRLEAETGQATGF
                     APIGFIEVAADKDRLEEYRRVAAFNRYCGVDVHEISPREVHELFPLARVDDIEAGFYV
                     REDGRVNPVDVTMALAKGARQRGVRIIEGVAVTDVLAERGRVQGVRTAHGDIRTDVVV
                     NCAGMWARQLAARSGITIANQAAEHYYLITEPIRDLPPNMPVLEDPAAYGYYREEGGG
                     LMVGLFEPVCAPWRIEGIPEDFSFGELPPDWERMTPFLERAMSRVPITGEVGMKKLFC
                     GPESFTPDLRPILGEAPELAGYFVAAGLNSIGVLTGGGVGRVLAHWIVTGRPDVDVTA
                     FNIDRLHPTQANPQYRRERTVESLGLVYKCHYPFMSLATARGVRCSPFHERLATAGAY
                     FREVSGWESPDWYAAPGTVPDPGPLTWGRPSWFGRWQEEHRAARERVIAMDMSFMGKF
                     LVQGRDAGRILNRISGNQVDGAAGVITYTQWLNEGGTLEADLTVTKLAEDRYLIVVTD
                     TMVRHAETWLRRHIPDDAQALVTDVTSAYGQLNVQGPLARALLQKLTSTDLANNAFPF
                     RTVREIDVGFARVLCIRITYIGELGYELYIPAEQAMHVYDRIVAAGAEFGLAHAGLKA
                     LGSLRMEKGYRDYGHDIDNTDGPYEAGLGFALDLKKPQGFIGKEAVLAHKARGPLTRR
                     LVQVLVKDPQPLMFHAEVLHRDGVPVGYLRAASYGHTLGGAVGLAMVESKVVVDADFL
                     GSGRWEVDIAGRRYPAAVSARPLYDPQMKRIHS"
     gene            complement(2356..3543)
                     /locus_tag="VI195_00925"
     CDS             complement(2356..3543)
                     /locus_tag="VI195_00925"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY6822974.1"
                     /translation="MLVHPNFDPVAFSVGPLAVRWYGLMYLLGFGLAWWLGLRRIAQG
                     AAPIDRRQFDDLIFYAVLGVILGHLAPPFLSLLDFARAAFARLHLPLYWQLGVGGLVV
                     GVISIFLPQVWGNGYSVVGTILRGELAGLTLLAVLGAKVLATSATVGSRAVGGVFTPT
                     LFIGCAVGALYGGVLHHALPSFTSVPAAYALIGMGGFLAATTHAPLTSIIMLFEMTAD
                     YQIVLPLMLACVVAHFTAKVYRRGESIYHASLTRALGADGVDDWRLRTIQPLVKPVAA
                     VTSSDTRLQQLLAKLPSRPVSRVYVTEGSKLLAWLDPREVLTALEQQRLTGDASVAAV
                     SHPVTFALTPQMSLSAALEGFLREQTATLPVTSDPWRNALLGEVSRADLLLAIQDRMT
                     YPK"
     gene            complement(3546..>4048)
                     /locus_tag="VI195_00930"
     CDS             complement(3546..>4048)
                     /locus_tag="VI195_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6822975.1"
                     /translation="SAEQAAALADGELDFGLLLPPVAGAAQLEHVVVQRERFVVALPA
                     RHRLARSRRVAMRALAGEPLVMVPKEIAPGLYDIVASLAARAGFSLNVAQEAIQMQTV
                     VSLVSSGLGAAIVPGSVANLGRRGVLYRELSDAHPRLDVWLAWPRATLEPAAQEFVAL
                     ARRRAP"
BASE COUNT          691 a         1346 c         1404 g          607 t
ORIGIN      
        1 cgggcaccac ctggcacgcg gctgggctca tggtcacctt cgggtcgacc tccgagacct
       61 cgaccgaaat gcgcaagtac gcgcgcgccc tctacagccg tctggaagct gagaccggtc
      121 aggcgaccgg atttgccccg atcggcttca tcgaggtggc ggccgataag gaccgcctgg
      181 aagagtaccg gcgcgtcgcg gccttcaacc gctactgcgg tgtcgatgtg cacgagatct
      241 ccccgcgtga ggtgcacgag ctctttccgc tcgcccgcgt ggatgacatc gaggcggggt
      301 tctacgtgcg cgaggacggc cgggtgaatc ccgtggatgt caccatggcg ctcgccaagg
      361 gtgcgcggca gcgcggagtg agaatcatcg agggtgtcgc ggtcaccgat gtgctcgccg
      421 agcgtggccg cgtgcagggc gtgcgcaccg cgcacggcga catccgcacc gacgtcgtgg
      481 tgaattgcgc cggcatgtgg gcacgacagc tcgccgcgcg ctccggcatc acgatcgcca
      541 accaggcggc cgagcactat tacctgatca cggagccgat ccgcgacctg ccgcccaaca
      601 tgccggtgct cgaggatccg gccgcctacg gttactaccg cgaggagggc gggggcctga
      661 tggtggggct cttcgagcca gtctgcgcgc cctggcgcat cgaaggcatc ccggaagatt
      721 tctccttcgg cgagctgccg ccggactggg agcggatgac gcccttcctg gagcgcgcca
      781 tgtcgcgcgt gccgatcacc ggcgaagtgg gtatgaagaa gctcttctgt ggaccggaga
      841 gcttcacgcc ggatctgcgc ccgattctcg gcgaagcgcc ggagctcgcg ggttacttcg
      901 tcgccgccgg tctcaattcc atcggggtgc tgaccggcgg tggggtgggg cgggtgctcg
      961 cgcactggat cgtgaccggc cgtcccgatg tcgatgtcac cgccttcaac atcgaccggt
     1021 tgcacccaac ccaggcgaac ccgcagtacc gccgcgagcg cacggtcgag tcgctcggcc
     1081 tggtctacaa gtgccactac cccttcatgt cgctcgccac ggcgcgcggc gtgcgctgct
     1141 caccgttcca cgagcgcctc gccaccgccg gcgcgtattt ccgcgaggta agcggctggg
     1201 aaagtcccga ctggtacgct gcgccgggta cggttccgga tccgggccca ctgacctggg
     1261 gacgcccgag ttggttcggg cgctggcagg aggagcaccg cgccgcgcgc gagcgcgtga
     1321 tcgccatgga catgtccttc atgggcaagt ttctcgtcca gggacgcgat gccggccgca
     1381 ttctcaaccg catctcgggc aatcaagtcg atggtgccgc aggcgtcatc acctataccc
     1441 agtggctgaa tgagggcgga acgctcgagg cggatctcac cgtcaccaag ctcgccgagg
     1501 atcgctatct catcgtcgtt accgacacca tggtgcggca tgcggagacc tggctccggc
     1561 gccacatccc cgacgatgcg caggcgctgg tgaccgatgt cacctcggcc tacggtcagc
     1621 tcaatgtgca ggggccactc gcgcgagcgt tgctgcagaa gctgacgagc acggatctcg
     1681 ccaacaatgc ctttccgttc cgcacggtgc gcgagatcga tgtgggtttc gcgcgggtgc
     1741 tgtgcatacg catcacctat atcggcgagc tgggctatga gctctacatc ccagccgagc
     1801 aggcgatgca cgtttacgac cgtatcgtcg cggcgggtgc ggagttcggg ctcgcgcacg
     1861 ctgggctcaa ggcgctcggc agcctgcgca tggagaaggg ctaccgcgac tacggacacg
     1921 acatcgacaa taccgacgga ccgtatgagg cgggtctcgg cttcgcgctc gacctgaaga
     1981 agccgcaggg cttcatcggc aaggaagcgg tactggcgca caaggcgcgc ggaccgctca
     2041 cccggcgcct ggtgcaggtg ctcgtcaagg acccgcagcc gctcatgttt cacgccgagg
     2101 tgctgcaccg cgacggtgtg ccggtcggct acctgcgtgc cgcctcctat ggccacacgc
     2161 tcggcggcgc cgtggggctc gcgatggtcg agtcgaaagt cgtagtcgat gcggattttc
     2221 tcggcagcgg ccgctgggaa gtcgacatcg ccgggcgccg ctacccagcg gcggtgtcgg
     2281 cgcggccgct ctacgatccg cagatgaagc gcatccacag ctgagtcgcg cgcgattgcg
     2341 agcgccggcc ggcgctcact tgggataggt catccggtcc tggatggcga gcagcagatc
     2401 ggcgcgcgat acctctccga gcagcgcatt gcgccacgga tcgctcgtta ccggcagggt
     2461 ggcagtctgc tcgcgcagga atccctcgag tgccgcagag agcgacatct gcggtgtcag
     2521 cgcaaaggtg accgggtgag agacggcggc gacgctcgca tcgcccgtca gccgctgctg
     2581 ctcgagtgcg gtaagcacct cacgcgggtc gagccaggcg agcagcttgc tgccctcggt
     2641 cacgtacacg cggctcaccg gccgggacgg cagcttcgcg agcagctgct gcaggcgcgt
     2701 gtcggatgaa gtcaccgctg ccaccggctt caccaacggt tggatggtgc gcagccgcca
     2761 gtcatcgact ccgtcggcac cgagggcgcg tgtgagcgag gcgtggtaga tggattcccc
     2821 gcgccggtag actttggcgg tgaagtgcgc cacgacgcac gccaacatca ggggcagcac
     2881 gatctggtag tccgcggtca tctcgaacag catgatgatc gaggtgagcg gtgcgtgggt
     2941 cgtggccgcc aggaacccgc ccatgccgat cagcgcgtag gccgcgggca ccgaggtgaa
     3001 ggagggcagc gcgtggtgca gcacgccgcc gtaaagcgcc cccaccgcgc agccaatgaa
     3061 aagggtcggg gtgaacactc cgccgaccgc acgtgagccc acggtcgcag aggtcgccaa
     3121 caccttggcg ccgagcacag cgagcagcgt gagaccggcg agctcgccgc gcagaatggt
     3181 gccgaccacg ctgtagccgt tgccccacac ctgcgggaga aatatcgaga tgacaccgac
     3241 cacgaggccg cccactccga gctgccagta gagcggcaaa tgcagccgcg cgaacgccgc
     3301 ccgggcgaaa tcgaggagac tcaggaatgg cggtgccaga tgtcccagga tcacaccgag
     3361 caccgcgtag aagatgaggt catcgaattg ccgacggtcg atgggcgcgg cgccctgcgc
     3421 gatgcgccgc agtccgagcc accaggcgag gccgaagccg agcaggtaca tgagcccgta
     3481 ccagcgcacg gcgagcggcc ccacggagaa cgcgaccgga tcgaaattcg gatggacgag
     3541 catgttcagg gagcgcgccg gcgcgcgagc gcgacgaatt cctgcgccgc cggctcgaga
     3601 gtcgcgcgcg gccaggccag ccagacatcg aggcgcggat gagcgtcgct cagctcccgg
     3661 tagaggacgc cgcgcctgcc gaggttcgcc acggaccccg gcacaatggc ggcgccgagg
     3721 ccgctcgata ccaggctgac tacggtctgc atctggattg cctcctgcgc tacgttgagg
     3781 gagaagccgg cgcgcgctgc caggctcgcg acgatatcgt acaggccggg cgcgatttcc
     3841 ttcggcacca tgacgagcgg ctcgcccgca agcgcgcgca tggcgacccg gcggctgcgc
     3901 gccaggcggt ggcgcgccgg cagcgcgacc acgaagcgct cgcgctggac aaccacatgc
     3961 tcgagttgcg cggcgcccgc gacgggtggc agcaacaggc cgaaatccag ctcgccgtcc
     4021 gccagcgcgg cagcctgctc ggcggaga
//
LOCUS       DATDMP010000052         2398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1008964, whole genome shotgun sequence.
ACCESSION   DATDMP010000052 DATDMP010000000
VERSION     DATDMP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008964"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..2258
                     /gene="iolC"
                     /locus_tag="VI195_00935"
     CDS             348..2258
                     /gene="iolC"
                     /locus_tag="VI195_00935"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEY6822976.1"
                     /translation="MNAQLDLITIGRVSVDLYGQQLGSRLEDAATFAKAVGGCPANVA
                     IGAARLGLSAALISRVGDEPMGRFVQEQLAREGVATRGLRIDPQRLTSLVLLGVRDEQ
                     TFPLIFYRDNCADSALGEEDIDEEFIASARAILVTGTHFSLAGGARAQHKAIEIAQAH
                     GRAVILDIDYRPNLWGIGGHGAGESRYAPSARVTTALAPVLRASQLIVGTEEELHIAG
                     GSEDTLEALRRIRTLSDAVIVCKRGARGCAVFAGQIPATFEEGLVTAGLDVEIYNVLG
                     AGDAFLAGFLSGYLRGLPHEESARRANACGALAVSRLLCSSEFATRAELDHLLAHGSA
                     HRALRHDPQLSHLHHVTTRRPHPGTLRALAIAGRAQLQPVSGTRNAVPDQLERLQRLA
                     LQAAARVAARREGFGILLDVPESGPAWREAVRSALWVARATHRQGSRPLEFASTDSLA
                     LRLTEWPIDTTVYCRCEWHADDPRELEEAQERRLKRLAAICRAQGREWLLEVAGAAPT
                     PFTDESLTRVLMRLYALDIRPDWWALEPQADRDAWDRCTRLISTNDPYCRGVLLTLAV
                     PAAAAAALTAAARVPLVRGFIAGGSIFAATTTAYLAGQLSDEAAVAAVAECFGDLVEA
                     WAAARDPKLDRP"
BASE COUNT          364 a          803 c          834 g          397 t
ORIGIN      
        1 attgcatgcg catctcgccc agggcgccgc gcacgcagat gccctgtatg cgatccacac
       61 acgtctcgcg cagcggctgc gggtcgagca cacgaccgtg cagatcgagg aggagggcac
      121 ctgccatagc ccggagtgcg gccccggccc ctgagcgggg cgatgcgcgc tgcggcggga
      181 ttcgctctca cagaagtcgc ccgaaattga tcctgtacct ctctgattca gcgcattgtt
      241 tccaggctta tgctcccgta gcgagcctcg gcggcgcctg aacctgtgat tgccggcggg
      301 gcgacgccgg tgtgcgttcg ccgcagggca caggcaatct gtcagccgtg aacgcgcaac
      361 tggatctgat caccatcggg cgcgtgagtg tcgatctcta tgggcagcag ctcggcagcc
      421 gcctggaaga tgccgcgacc ttcgccaaag ccgtgggtgg gtgcccggcc aatgtcgcga
      481 ttggggccgc ccggctcggg ctgagcgctg cgctcatcag ccgggtgggc gatgagccga
      541 tggggcgctt cgtgcaggaa cagctcgcac gcgagggcgt cgcgacgcgc ggcctgcgca
      601 tcgacccgca gcgcctcacc tcgctggtgc ttcttggcgt gcgcgatgag cagaccttcc
      661 cgctcatctt ctatcgcgac aactgtgccg acagcgcctt gggtgaggaa gacatcgatg
      721 aggagttcat cgcctccgcg cgcgccatac ttgtgaccgg gacgcatttc tcgctggccg
      781 gaggcgcacg cgcacagcac aaggcgatcg agatcgccca ggcacacggg cgcgcggtaa
      841 tcctcgatat cgactaccgc cccaatctct ggggcatcgg cggccacggc gcgggtgaat
      901 cccgctacgc accctccgca cgggtgacta ctgcgctcgc tccggtgctg cgcgctagcc
      961 aactcatcgt cggcacggag gaagagctgc acatcgccgg ggggagcgag gacaccctcg
     1021 aggcgctgcg gcgcatccgc acgctgtctg acgccgtgat cgtctgcaag cgcggcgccc
     1081 gcggctgcgc ggtgtttgca gggcagattc cggctacctt cgaggagggg ctggtcacgg
     1141 ccggactgga tgtcgagatc tacaacgttc tcggagccgg ggatgcgttt ctcgccgggt
     1201 tcctgagcgg gtacctgcga ggcttacctc acgaggagag cgcgcggcgc gccaatgcct
     1261 gcggtgcgct ggcggtgtcg cgcttgctgt gctcgtccga atttgcgacg cgcgcggagc
     1321 tcgatcactt gctcgcccac ggcagcgcgc accgcgcttt gcgccacgat ccgcagctca
     1381 gccatttgca ccacgtgacg acccgccgtc cgcaccccgg cacgctgcgg gcgctcgcca
     1441 ttgcagggcg cgcgcagcta cagcccgtga gcggcacgcg caatgcagtg cccgaccagc
     1501 tcgagcggct gcagaggctc gcactgcagg ctgccgcgag ggtcgccgcg cgccgcgagg
     1561 gctttggaat cctgctcgat gtccctgaga gtggcccggc ctggcgggag gcggtgcgca
     1621 gtgcgctgtg ggtcgcccga gcgacgcatc ggcaaggctc gcgaccgctc gagtttgcat
     1681 ccaccgatag tctcgcgctg cgcctgacgg agtggcccat cgacaccacg gtctactgcc
     1741 ggtgcgagtg gcacgcggac gacccccggg agctggagga ggctcaggag cgcaggctca
     1801 agcgtctcgc agcgatctgc cgcgcgcagg ggcgagaatg gctgctggag gtcgcaggcg
     1861 ccgcaccaac tcccttcacg gacgaatcgc tcacacgggt gctcatgcgc ctttacgcgc
     1921 tggacattcg tccggactgg tgggcgctcg agccacaagc cgaccgcgat gcctgggacc
     1981 gatgcacgcg actgatttcc acaaacgatc cctactgccg cggtgtgctg ctcacgctgg
     2041 ctgtgcctgc ggccgctgcc gctgcgttga ctgccgcggc acgggttcct ctcgtgcgcg
     2101 gcttcattgc cggcggtagc atattcgccg ctaccacaac ggcgtacctt gcgggtcagc
     2161 tgtcggacga ggcggcagtc gccgcggtgg cggagtgctt cggagatctg gtcgaggcgt
     2221 gggccgcggc ccgggatccc aagctcgata gaccctaaag gagctcgaat cgatggcgct
     2281 gcggctcacg gtggcacaag ccctggtgca ggccctggcc gcacagtgga cgctcatcga
     2341 tggcgagcgg cggcggctgt ttgcgggagt ctgggcgata ttcggccacg gcaacgtc
//
LOCUS       DATDMP010000053         4641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1119902, whole genome shotgun sequence.
ACCESSION   DATDMP010000053 DATDMP010000000
VERSION     DATDMP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119902"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VI195_00940"
     CDS             complement(<1..571)
                     /locus_tag="VI195_00940"
                     /inference="COORDINATES: protein motif:HMM:NF004130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY6822977.1"
                     /translation="MAIEISARKREAQGTGASRRLRRTGRVPGIVYGGDKGPTNIELN
                     HQDLFLHLRNEKFHASILMLDIAGAKEQVLLRAVNMHPYKLEVQHIDFQRVSKDKKIH
                     MKVPLHFVNAEKSPGVKEQAGVVNHVLNELDIICFPDDLPEFIEVDLSSLTVGHSIHL
                     RDLALPKGVEAAVHKGENPVVATVVIPALV"
     gene            complement(637..1599)
                     /locus_tag="VI195_00945"
     CDS             complement(637..1599)
                     /locus_tag="VI195_00945"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008307606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY6822978.1"
                     /translation="MNTSAPLDRLMVFTGNANPRLAHDVAKHLNLSIGRAIVGKFSDG
                     EVMVELLENVRGKDVFVLQPTCAPTNDNLMELLVMVDALKRSSAARVTAAIPYFGYAR
                     QDRRPRSARVAISAKVVANMLTSVGVARVLTMDLHADQIQGFFDLPVDNIYAAPVLLG
                     DVWKQRYENLIVVSPDVGGVVRARALAKRLESDLAIIDKRRPKANVSEVMNVIGEVKD
                     RTCVIMDDMVDTAGTLVKAAEVLKEEGATKVVAYCTHAVLSGSAIERIQGSSLDELVV
                     TDTIPLRDEGRACKKIRQLSVAGLIAETILRIYTEESVSSLFIE"
     gene            complement(1613..1689)
                     /locus_tag="VI195_00950"
     tRNA            complement(1613..1689)
                     /locus_tag="VI195_00950"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Gln,seq:ttg)
     gene            complement(1702..2541)
                     /gene="ispE"
                     /locus_tag="VI195_00955"
     CDS             complement(1702..2541)
                     /gene="ispE"
                     /locus_tag="VI195_00955"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY6822979.1"
                     /translation="MELTWYPAPAKLNLFLHVLGRVAQGEHAGYHDLQTVFRLIDRSD
                     RIGIAPREDAEVRFSGPFGEENLCARAARLLREQAGVQRGCEIHLEKNLPVGGGLGGG
                     SSDAATVLLVLNRVWKLSWKREKLLRLGRSLGADVPFFIYGKNALGEGIGERLKALQL
                     APAWYLVLTPQVAVSTKEIFSDAALTRDTKRLTIPPFFSGQGANDLEAVVCARYPEVA
                     AHLAWLRERCPQARMTGSGACVFAEFPNQAEALAVHSKMPGGMGGFVAQGLERHPLHD
                     WAD"
     gene            complement(2532..3002)
                     /gene="lolB"
                     /locus_tag="VI195_00960"
     CDS             complement(2532..3002)
                     /gene="lolB"
                     /locus_tag="VI195_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY6822980.1"
                     /translation="MRPLSSEVLFDISGRLAARYADESFTGGFTWRHAEADDEMLITS
                     PLGQGVARIAREGGSVVLTTAEPREYRASDAEALTAQVLGFRLPLAGLSDWVRGAASA
                     ASPATEEKDAQGRLKKLQQAGWQIEYQDYAGGHPSRMRLTYPGIELRLAISKWN"
     gene            complement(3064..>4641)
                     /locus_tag="VI195_00965"
     CDS             complement(3064..>4641)
                     /locus_tag="VI195_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6822981.1"
                     /translation="LDEATLYELLVGEIALQRGDAALAAQTYAELARSTRDPRIVRRA
                     IEVANFAHQPELALEAAKLWSDMEPQSPQPLQVIAAILIAGKRVDEAAPYLQKLLAAD
                     GVNLENGYMQLNRLLAGNPDKASNLRVVRELAATHPELAQAHFAVAQAALVAGDEAVA
                     LAEIRQAQAIRPDWENAVILQAQVLQKRSPAEAAKVLGDFVEKHPASNEARLNYARAL
                     VLDKRFPEARKQFEAVLAANPGNTDVVYAVGLLALQLKDYPVAEENMKRLLSMNYRDP
                     NGVRYLLGQIAEEQKQWPRAIEWYGQIDEGEHALGARMRTASVMAKEGKVDEARAYLR
                     RAAAENPDQEAQITVAEAQLLRDANRVRDAYEVLGQALKKEPDQPDLLYDYALTAEKL
                     QRYDVLENNLRKLMEVKPDHAHAYNALGYSLVERNTRLPEAKKLIQHALELAPDDAFI
                     VDSMGWLLYREGDLKAAADELRRAYNSRPDAEIGAHLGEVLWVMGQRDEARQVWQESL
                     KASPDNESLIKTIQRFR"
BASE COUNT          743 a         1524 c         1512 g          862 t
ORIGIN      
        1 ccaccagcgc cgggatgacc acggtggcca ccacggggtt ctcgcccttg tgcaccgcgg
       61 cttccacgcc cttcggcagc gcgagatcgc gcaagtggat cgaatggccg acggtgagcg
      121 agctcaggtc cacctcgatg aattccggca ggtcatccgg gaagcagatg atgtcgagct
      181 cgttcagaac gtggttcacg acgcccgcct gctccttgac gccgggagac ttctccgcgt
      241 tcacgaagtg cagcggcacc ttcatgtgga ttttcttgtc cttggagacg cgctggaaat
      301 cgatgtgctg cacctcgagc ttgtacgggt gcatgttgac cgcgcgcaac agcacctgct
      361 ccttggcgcc cgcgatgtcc agcatgagga tcgaggcgtg gaatttctcg ttccgcagat
      421 gcaggaacag gtcctggtgg ttgagctcga tgttggtcgg ccccttgtct ccgccgtaca
      481 cgatcccggg aacgcgcccg gtccggcgca ggcggcggct cgcacccgtt ccttgcgctt
      541 cgcgctttcg cgcgctgatt tcaatggcca tcgtagtgct ccttgtcgat tcgccggctc
      601 cgcgaccgaa gacaggcgaa ggttaaaaaa ccttaactat tcgataaaca ggctgctcac
      661 cgattcctcg gtgtagatcc tcaggatcgt ctcggcgatg aggccggcga cggagagctg
      721 gcggatcttc ttgcaggcgc gtccctcgtc gcgtagcggg atcgtgtcgg tcacgaccag
      781 ctcgtcgagg ctcgagccct ggatgcgctc gatggcgctg cccgaaagca ccgcgtgcgt
      841 gcagtaggcc acgaccttgg tggcgccttc ctccttgagc acctcggccg ccttcacgag
      901 cgtgcccgcg gtgtcgacca tgtcgtccat gatcacgcag gtgcggtcct tgacctcgcc
      961 gatcacgttc atcacctccg agacgttcgc cttcgggcgg cgcttgtcga tgatggcgag
     1021 gtcggattcc agtcgcttgg cgagcgcccg ggcgcgaacc acgccgccga catcgggcga
     1081 aaccacgatg agattctcgt agcgctgctt ccacacgtcg ccgagcagga ccggcgcggc
     1141 gtagatgttg tctaccggaa gatcgaagaa accctggatc tggtccgcgt gcaggtccat
     1201 ggtcagcacg cgcgcgacgc caacggaggt cagcatgttg gcgaccacct tcgcactgat
     1261 tgcaacccgc gccgagcggg gccggcgatc ctggcgcgcg tagccgaaat acgggatggc
     1321 agccgtcacc ctggcggcag acgagcgctt gagcgcgtcc accatgacca gcagctccat
     1381 caggttgtcg ttggtcggcg cgcaggtcgg ctgcagcacg aacacgtctt tgccgcgcac
     1441 gttctccagc agctcgacca tcacttcgcc gtccgagaac tttccgacga tggcgcggcc
     1501 gatcgagagg ttcaggtgct tggcgacgtc gtgcgccagc cggggattcg cgttccccgt
     1561 gaacaccatc aaccgatcaa gcggtgcgct cgtattcatg tctcaggata agtggctggg
     1621 gaggaaggat tcgaaccctc gtatgccgga atcaaaatcc ggtgccttaa ccagcttggc
     1681 gactccccag ttgtgttcaa ttcaatctgc ccagtcatga agcgggtgac gctcgagccc
     1741 ctgcgccacg aatccgccca tcccccccgg catcttggag tgaaccgcga gcgcttcggc
     1801 ttggttcgga aactcggcga agacgcacgc tccggagccc gtcatgcgcg cctgcggaca
     1861 ccgttcccgg agccacgcga ggtgcgctgc cacctcgggg tagcgcgcgc aaaccacagc
     1921 ctcgagatcg ttcgcgccct gccccgagaa aaagggcggt attgtgagtc gtttcgtatc
     1981 gcgtgtcaac gcagcgtcgg agaagatttc cttcgtcgaa accgcaactt gcggcgtcaa
     2041 aacgaggtac caggcggggg caagttgaag cgctttcagg cgctcgccga tgccttcgcc
     2101 aagggcgttt tttccgtaga tgaaaaaggg cacgtctgca ccgagcgagc gcccgagccg
     2161 caggagtttt tcccttttcc acgagagctt ccagactctg ttgagcacga ggagcacggt
     2221 cgccgcgtcg gaggagccgc caccgaggcc gccgcccacg ggcaggttct tttccaggtg
     2281 aatctcgcaa ccgcgctgca ccccggcctg ttccctcaag agtcgcgccg ctcgcgcgca
     2341 cagattctct tcgccgaatg gtccgctgaa acgcacttcc gcgtcctcgc gcggcgcgat
     2401 gccaatgcga tcggagcggt cgatgaggcg gaacaccgtc tgcaggtcgt gatagcccgc
     2461 gtgttcgcct tgcgcaacac gacctaagac gtgcaggaac aggttcagct tcgccggcgc
     2521 cgggtaccag gtcagttcca tttcgagatc gcgaggcgca gctcgatgcc ggggtaggtg
     2581 aggcgcatgc gcgagggatg tccgccggcg taatcctggt actcgatctg ccacccggct
     2641 tgctgcagct tcttcagccg cccttgcgcg tccttctctt cggttgcggg cgaggcagcc
     2701 gaagccgcgc ctcgcaccca atcggaaagc ccggcgagcg gcaggcggaa gccgagcacc
     2761 tgcgccgtca gcgcttccgc atcgctcgcg cgatattcgc gcggctccgc ggtcgtgagg
     2821 accacgctgc cgccctcgcg cgcgatgcgc gcgacgccct gtccgagcgg gctggtaatc
     2881 agcatttcgt cgtccgcctc ggcgtggcgc caggtgaaac cgccggtgaa cgattcgtcg
     2941 gcgtagcgcg cggcgaggcg gccgctgatg tcgaacagca cctcggaaga aagcggccgc
     3001 acgggagcct ggacgcaagc gccgaggaga agcgcaagca gcagccaggc gcgccgcatt
     3061 aggtcagcgg aagcgctgga tcgtcttgat cagcgactcg ttgtccgggc ttgctttcag
     3121 cgattcctgc cacacctgcc gcgcttcgtc gcgctggccc atgacccaca gcacctcccc
     3181 aagatgcgcg ccgatctcgg cgtccggccg gctgttgtag gcgcggcgca gctcatccgc
     3241 ggcagctttc aggtcgcctt cgcggtaaag cagccagccc atgctgtcca cgatgaacgc
     3301 gtcgtccgga gcgagctcga gcgcatgctg gatgagcttc ttggcctcgg ggagccgcgt
     3361 gttgcgctcg accagcgaat agccgagcgc gttgtaggcg tgcgcatggt ccggctttac
     3421 ttccatgagc ttgcgcaggt tgttctccag gacgtcgtag cgctgcagct tctctgccgt
     3481 aagcgcgtag tcgtagagca ggtcgggctg gtcgggctct ttcttgagcg cctggcccag
     3541 cacttcgtag gcatcgcgga cgcggttggc atcgcgcagg agctgcgcct cggcgacggt
     3601 gatctgcgcc tcctggtcgg gattctcggc cgccgcgcgg cgcagatagg cgcgcgcttc
     3661 gtcgaccttt ccctccttcg ccatgacgct cgcggtgcgc atgcgggcgc cgagcgcatg
     3721 ctcgccttcg tcgatctggc cataccattc gatggcgcgc ggccactgct tctgttcctc
     3781 cgcgatctgc ccgagcaggt agcgcacgcc gttcggatca cggtaattca tcgagagcag
     3841 ccgcttcatg ttctcttcgg cgaccggata gtccttgagc tgcagcgcca gcagcccgac
     3901 tgcgtaaacc acgtcggtgt tgcccggatt ggcggctagc acggcctcga attgcttgcg
     3961 cgcctccgga aagcgcttgt cgagaaccag cgcccgggcg tagttgaggc gcgcttcatt
     4021 ggaggcgggg tgcttctcca cgaaatcgcc cagcaccttc gcggcctccg ccggcgagcg
     4081 cttctgcagc acctgcgcct gcaggatgac ggcgttctcc cagtccgggc ggatggcttg
     4141 ggcctggcgg atttcggcga gagcgacggc ctcgtcgccc gccaccagcg cggcttgcgc
     4201 gacggcgaag tgcgcctggg cgagctccgg gtgcgtcgcc gcgagctcgc gcaccacgcg
     4261 caggttggac gccttgtccg gattccccgc cagcaagcga ttgagctgca tgtagccgtt
     4321 ctcgaggttc acgccatcgg cggcgagcag cttttgcagg tacggcgcgg cctcgtcgac
     4381 gcgctttccg gcgatcagga tcgcagcgat gacctggagc ggctgcggag attgcggctc
     4441 catgtcggac cagagcttgg cggcctcgag cgcaagctcg ggctggtgcg cgaagttcgc
     4501 gacttcgatg gcgcgccgca cgatgcgggg atcgcgcgtg gagcgcgcga gctcggcgta
     4561 ggtctgcgcc gcgagcgcgg cgtcgccacg ctgcagggcg atctcgccta cgagaagctc
     4621 gtagagcgtc gcttcatcga g
//
LOCUS       DATDMP010000054         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1231390, whole genome shotgun sequence.
ACCESSION   DATDMP010000054 DATDMP010000000
VERSION     DATDMP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231390"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VI195_00970"
     CDS             complement(<1..1108)
                     /locus_tag="VI195_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6822982.1"
                     /translation="MPDSVYGHFSRAFRGPPDVQEAGRRAYNLFMSDNGLFALRADYI
                     RRTEEELIGMCVGLFHPPADASGTFTSGGSESNYTVIHAMREWARERFPEIQTPELIV
                     PFSAHPTFSKGCHYYGIRLVRTPLGEDRRADPRAIAQAINANTIGVAGSAPCWPYGLM
                     DPIEELGELASRHSLWLHVDACVGGYLAPFAERLGVRLPKWDFRVPAVMSISADLHKY
                     GYCPKPASTVLWRSEGLKRFHHVHPEDWPGGPYHMQGFAGSRSAGPIFAAWTVLRYLG
                     IEGYERLARRVLDTQQQLRAAIEAIEGLYTLENDLPPLAFGSHSLDMALIMSQLRKAG
                     WILVASRQPPLINLPIDPALDERICAAFIGHLEAA"
     gene            1270..>2003
                     /locus_tag="VI195_00975"
     CDS             1270..>2003
                     /locus_tag="VI195_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY6822983.1"
                     /translation="MVLGEWLRACGPAGVALGMLAGGGIMLANSGAYAELIARFPVAG
                     GEFTFVRQVFGDRSAFMVGWLWTLSLLAVVAFEATALPWLLEALVPVLRGPVLYRSLN
                     TPVSADALAIGLGGTIVIAVLNYRGIRSAATLQSVLAIAFMGLAIVIVAAGIALGSWH
                     NAEPLVSGAGGRPWWMGSLWIFAITPFFLNGFQSVAQTVEERGARVTFTGIARAMVLA
                     IVIAIVFYCLITFAAASATPWQSLIDR"
BASE COUNT          314 a          626 c          674 g          389 t
ORIGIN      
        1 cggcggcttc gagatggccg atgaaggctg cgcagatgcg ctcgtcgagc gccggatcga
       61 tcggcaggtt gatgagcggc ggctggcgcg aggcgacgag aatccagccc gccttacgca
      121 gctgactcat gatgagtgcc atgtcgagcg aatgcgagcc gaatgccagc ggcggcagat
      181 cgttctcgag ggtgtaaagc ccctcgatcg cctcgatggc tgcacgcagc tgctgctgcg
      241 tgtcgagcac ccggcgcgcg agccgctcat aaccttcgat gccgaggtac ctgagcaccg
      301 tccatgcggc gaagatgggg cctgcggacc ggctcccggc gaatccctgc atgtgatacg
      361 gcccgcccgg ccagtcctca gggtgcacgt ggtgaaagcg cttgaggcct tctgaacgcc
      421 acagcacggt cgaggcaggt ttcgggcagt agccgtactt gtgcaggtcc gcggaaatcg
      481 acatgaccgc gggaacgcgg aagtcccact ttgggagcct cactccgagc cgttccgcga
      541 acggtgcgag atagccgccg acgcaggcgt cgacgtgcaa ccacaggctg tgccgggaag
      601 cgagctcgcc gagctcctcg atcgggtcca tcaatccata gggccagcaa ggcgctgatc
      661 ctgcgacgcc gatagtgttg gcgttgatgg cctgcgcgat ggcgcgcggg tccgcgcgtc
      721 gatcctcgcc aagcggcgtg cgcacgagcc ggatcccgta gtagtggcat cccttcgaaa
      781 aggtcggatg cgcggagaag gggacaatga gctccggtgt ctgaatctcc ggaaaccgct
      841 cacgggccca ctcgcgcatc gcgtggatca cggtgtagtt gctctcggaa ccgccactcg
      901 tgaacgtccc cgaggcgtct gccggcgggt gaaacaggcc gacgcacatg ccgatgagct
      961 cctcctcggt acgccggatg tagtccgcgc gcagcgcgaa aaggccgttg tctgacatga
     1021 acaagttgta ggcgcgacgg ccggcttcct gcacgtccgg cggtccgcga aacgcgcggc
     1081 tgaaatggcc gtacacggag tccggcaacc gcgactggag ctccgcttcg aggtttggga
     1141 gggggacgcc tctttccgga agctcgcatc gattctgatt catggtagtc tcggctgcgc
     1201 ctgcgcgtcc ttaatcctat acagatcttc gcattatcct tcggctgcat catcggctcc
     1261 ggctgggtcg tggtgctcgg ggagtggctg cgggcgtgcg gtccggccgg ggttgccctc
     1321 ggcatgctgg cgggcggcgg catcatgctc gccaatagcg gcgcctatgc cgagttgatc
     1381 gcacgtttcc cggtggcggg cggtgaattc accttcgtcc ggcaggtgtt tggcgatcgc
     1441 tccgccttca tggtgggctg gctctggacc ctgtcgctgc tcgctgtagt cgccttcgag
     1501 gcgacggccc tgccgtggct gctcgaggcc ctggtgccgg tgctacgggg accggtgctg
     1561 tacaggtcgc tcaacacccc ggtctcggcc gatgcgctcg cgatcgggct tggcggcacg
     1621 atcgtcattg ccgtactcaa ttatcgcggt atccgcagcg cagccaccct gcaatcggtg
     1681 cttgcgatcg ccttcatggg tcttgccatc gttattgttg ccgccggaat cgcgctcggc
     1741 tcctggcaca acgccgagcc actcgtgagt ggcgcggggg gacggccctg gtggatggga
     1801 agcctctgga tcttcgcgat cacgcccttt ttcctcaatg gctttcagtc agtggctcag
     1861 accgtcgaag agcgcggcgc ccgcgtcact tttacgggca ttgcgcgcgc aatggtgttg
     1921 gcgatcgtga ttgccatcgt gttctactgc ttgatcacct tcgctgcggc aagcgcaact
     1981 ccgtggcagt cgctcatcga tcg
//
LOCUS       DATDMP010000055         2073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2008606, whole genome shotgun sequence.
ACCESSION   DATDMP010000055 DATDMP010000000
VERSION     DATDMP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008606"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VI195_00980"
     CDS             complement(<1..986)
                     /locus_tag="VI195_00980"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY6822984.1"
                     /translation="MSLSVFDIFKVGIGPSSSHTMGPMRAAAEFVAGLKRDGLLAATQ
                     EIAVRLYGSLALTGVGHGTDRAILAGLEGALPENADPDHIEATVRRIRASGRVRLLDE
                     HEIAFDEPMQLLFMQHERLPRHSNGMRFTALAADQRVLREEEYYSIGGGFIVRGAETE
                     AAGPHERAQPPYPFTSAAQLLALCREHGLEIFELVLANERAWASESQVRAKLLEIWQV
                     MQQCVERGFRQSGVLPGVLKVRRRAPRLYRMLTECATLKPLEVMDWVNAYALAVNEEN
                     AAGGRVVTAPTNGAAGIIPAVLSYYQRFIPNANDDGIQRFLLTAAAIAILYKT"
     gene            1280..>2073
                     /locus_tag="VI195_00985"
     CDS             1280..>2073
                     /locus_tag="VI195_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6822985.1"
                     /translation="MNQTLLERLRSELAALRDQGLYKSERVIAGPQGAVVHAGDRDLI
                     NLCANNYLGLANHPAVRAAAERALGELGYGMASVRFICGTQQIHKTLEARLARYLGSE
                     DVILYSSCFDANGGLFETLLDERDAVISDALNHASIIDGIRLCKAQRYRYANSDMAEL
                     EQCLRQAQGARTRLIATDGVFSMDGFIARLREICDLAEKYQALVMVDDSHATGFMGPS
                     GRGTPEHCGVAQRVDIITSTLGKALGGGSGGFVAARREVIEWLRQRS"
BASE COUNT          345 a          671 c          719 g          338 t
ORIGIN      
        1 gtcttgtaga ggatggcgat cgcggcggcc gtgaggagga agcgctggat gccgtcgtcg
       61 ttcgcgttgg ggatgaagcg ctggtagtag gagaggacgg cggggatgat gcccgccgcg
      121 ccgttcgtcg gcgcggtcac gacgcgcccg ccggcggcgt tttcctcgtt gaccgcgagc
      181 gcgtaggcat tcacccagtc catgacttcg agcggcttca gcgtggcgca ctcggtgagc
      241 atgcggtaca ggcgcggtgc gcggcggcgc accttgagga ccccgggaag cacgccgctc
      301 tgccggaagc cccgctcgac gcactgctgc atcacctgcc agatctcgag cagcttggcg
      361 cgcacttgcg actcgctagc ccaggcgcgc tcgttggcga gcaccagctc gaagatctcc
      421 agcccatgct cgcggcagag cgcgagcagc tgtgccgccg aggtgaacgg atacggcggc
      481 tgcgcgcgct cgtggggacc agccgcctcg gtctcggcgc cgcgaacgat gaaaccgccg
      541 ccgatcgagt agtactcctc ttcgcgcagc acgcgttggt ccgcggcgag cgccgtgaag
      601 cgcatgccgt tggaatgccg cggcaagcgc tcgtgctgca tgaaaagcag ctgcatcggc
      661 tcatcgaagg cgatctcgtg ctcatcgaga agccgcacgc ggccgctggc gcggatgcgc
      721 cgcacggtgg cctcgatgtg atccggatcg gcattctccg gcagtgctcc ctcgagcccg
      781 gcaaggatgg cgcgatcggt gccgtgaccg acaccggtga gagcgagcga tccgtacagg
      841 cggaccgcga tctcctgggt cgcggcaagg agaccatcgc gcttgaggcc ggcgacgaac
      901 tccgcggcgg cccgcatcgg ccccatggtg tgggagctcg aggggccgat gcccaccttg
      961 aagatgtcga agacgctcaa gctcatgaga accaaccggt ctatcaggta cggcggcacc
     1021 tgcgcccgct agatgggggt atacgcagca gtctgcaacg cgtgagcgcc gccgacatgc
     1081 tttgcggcga cctcggggga ctcaaatgcg accctccgga gctggcgggg ctcacgatgc
     1141 gtagggtctt tacccgaaca gttcaccaat tggtgaacta ttcttcagac ggctctatgg
     1201 cacgcgctag ctgctcgagc gatggcggct gtttgatcgc cgcggcgtcg ggagggaggg
     1261 cttgggtata atccccctta tgaatcaaac ccttctcgag cggctgcgga gcgagcttgc
     1321 cgcgcttcgc gaccagggac tctacaagag cgagcgtgtg atcgccggtc cccagggggc
     1381 ggtggtgcac gctggcgatc gcgacctcat caacctgtgc gccaacaact atctcgggct
     1441 cgccaatcac ccggcggtgc gcgccgccgc ggagcgggca ctcggggagc tcggctacgg
     1501 catggcctcg gtgcgcttca tctgcggcac gcagcagatc cacaagaccc tcgaagcgcg
     1561 cctggcgcgc tatctcggca gcgaggatgt gatcctctac tcctcctgct tcgatgccaa
     1621 cggcggactg ttcgagacgc tgctcgatga acgcgacgcg gtgatctcgg atgcgctcaa
     1681 tcatgcgagc atcatcgacg gcatccgcct gtgcaaggcg cagcgctacc gctacgccaa
     1741 cagcgacatg gcggagctcg agcagtgtct gcgacaggcg cagggagcgc gcacgcggct
     1801 catcgccacc gatggcgtct tctccatgga cgggttcatc gcgcggctgc gcgagatttg
     1861 cgacctggcg gagaaatacc aggcgctggt catggtcgat gactcccatg ccaccggctt
     1921 catgggaccg agcgggcgcg gtacgcccga gcactgcggc gtggcgcaac gggtcgacat
     1981 catcaccagc acgctcggta aggcgctcgg cggcggcagc ggcggcttcg ttgcggcgcg
     2041 ccgcgaggtc atcgagtggc tgcgccagcg ctc
//
LOCUS       DATDMP010000056         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1564655, whole genome shotgun sequence.
ACCESSION   DATDMP010000056 DATDMP010000000
VERSION     DATDMP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564655"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VI195_00990"
     CDS             <1..1150
                     /locus_tag="VI195_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY6822986.1"
                     /translation="DIHNVHVVGAGVMGGDIAAWSALRGFSVTLEDRAAEYIEPAMKR
                     AQELFDKRLRDPAQNAAARTRLRADVAGEGVAAADLVIEAIFENLTAKQELYARLEPR
                     MKPGALLATNTSSIMLEPLGARLAHPERLVGLHFFNPVAQMPLIEIVHAERTAPGAVQ
                     AASGFARRLDKLPVSCRSAPGFMVNRVLTPYLYEAMLAAEEGTALAVIDRCAVSFGMP
                     VGPIELIDVVGLDVASHVGEIIAQELRRPVTQIARLSELLAAKKLGRKSGEGFYLWRE
                     GKAVKPAVEDASAPPDLLDRLILVMINECVACLRERVVESADLVDAAVLFGTGFAPFR
                     GGPLNYARARGISALVARLKELAARHGARFDPDEGWISLGADQKRDGA"
     gene            1242..>2095
                     /locus_tag="VI195_00995"
     CDS             1242..>2095
                     /locus_tag="VI195_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY6822987.1"
                     /translation="MTEEQAASIQLLRMFAPLDGMKRENLAALAKKVSVRTLTRGRVL
                     FSQGDSDKRTFWLVSGTVEVDDGQKRVTVLRAGTPEAKNPLHPSLPRTVTVRAADDIS
                     FLSIDSDLLDVMITWDQTGSYEVEELQAQLQGAGSDDWMTTLLQTNAFHRIPPANIQA
                     IFQRLQRTPCRAGEVVIKQGAEGDYFYIIVSGKCAVTRETPLSRDGIKLAELGVGDTF
                     GEEALIAEAKRNATVTMLTDGVLMRLNKQDFRELMNEPLLQWVTRAQADEIIARGGRW
                     LDVRLPSE"
BASE COUNT          364 a          688 c          711 g          332 t
ORIGIN      
        1 cgacatccat aacgtgcacg ttgtcggcgc cggcgtcatg ggcggagaca tcgcggcctg
       61 gtcggcgttg cgcggcttca gcgtcacgct cgaggaccgc gccgcggaat acatcgagcc
      121 ggcgatgaag cgcgcacagg agctgttcga caaacgcttg cgcgatcccg cacagaatgc
      181 tgccgcgcgc acgcggctgc gcgccgatgt cgcgggcgag ggtgtggcgg ccgcggacct
      241 ggtgatcgaa gccatcttcg agaatctcac ggccaagcag gagctctatg cgcgcctcga
      301 gccgcgcatg aaacccggcg ctctgctcgc gaccaacact tcgagcatca tgctcgagcc
      361 gctcggcgcg cgccttgccc atcccgagcg cctcgtgggg ctgcacttct tcaatccggt
      421 ggcgcagatg ccgctgatag agatagtgca tgccgagcgc acggcaccgg gcgcagtgca
      481 ggccgccagc ggcttcgcgc ggcgcctcga caagctgccg gtgtcctgcc gcagcgcgcc
      541 gggcttcatg gtgaaccgtg tgctgacacc gtacctgtac gaggcgatgc tcgccgccga
      601 ggagggaacg gcgctcgcgg tcatcgaccg ttgcgcggtg agcttcggca tgccggttgg
      661 gccgatcgag ctgatcgatg tggtcggact cgacgttgcc tcccatgtcg gtgagatcat
      721 cgcgcaggag ctccgccggc cggtgacgca gatcgcacgt ctcagcgagc tgctcgccgc
      781 caagaagctc ggccgcaaga gcggcgaagg cttctatctc tggcgcgagg gcaaggcggt
      841 taaacctgcg gtcgaggacg cgagtgcgcc gcccgatctg ctcgaccgtc tgatactcgt
      901 catgatcaat gagtgcgtgg cctgcctgcg cgagcgggtc gtggagagcg ccgatctcgt
      961 cgatgctgcg gtgctgttcg gtaccggctt cgcccccttc cgcggcggcc cgctcaacta
     1021 cgcgcgcgcc cgcggcatca gcgcgctcgt ggcccggctc aaagagctcg ccgcgcgcca
     1081 cggtgcgcgc ttcgaccccg atgagggctg gatctcactc ggggccgatc aaaagcgtga
     1141 cggggcctga agcgcacgca aaccgctgtg ttaccatcaa aaactcatcg ctccgccggc
     1201 tgcgcgagtg cgatgtggtt cacgtttcgc gttggggaaa aatgaccgaa gaacaggcag
     1261 cgtccatcca gttgctgcgc atgtttgcac cgctcgatgg catgaagcgc gagaatctcg
     1321 ccgcgctcgc caagaaagtg agcgtacgca cgctgacgcg cgggcgcgtg ctgttcagcc
     1381 agggcgatag cgacaagcgc accttttggc tggtgagcgg cacggtcgag gtcgatgacg
     1441 gccagaagcg ggtgaccgtg ctgcgcgccg gcacccccga agccaagaac ccgctgcacc
     1501 cgagtctgcc gcgcacggtc accgtgcgcg cggccgatga catcagcttc ctgtcgatcg
     1561 acagcgatct gctcgatgtc atgatcacct gggaccagac cggcagctat gaggtcgagg
     1621 agctgcaggc gcagctgcag ggcgcgggct cggatgactg gatgacgaca ctgctgcaga
     1681 ccaacgcctt ccatcgcata ccgcccgcga acatccaggc gatatttcag cgtctgcagc
     1741 gcactccgtg ccgggccggc gaggtggtga tcaagcaggg cgcggagggc gattacttct
     1801 acatcatcgt cagcggcaag tgcgcagtga cgcgcgagac cccgctatcg cgcgatggca
     1861 taaagctcgc cgagctcggc gtcggcgaca ccttcggcga ggaagccctg attgccgaag
     1921 ccaagcgcaa cgccaccgtc acgatgctca ccgacggcgt gctgatgcgg ctcaacaagc
     1981 aggacttccg cgagctcatg aacgagccgc tgctgcagtg ggtgacgcgc gcgcaggccg
     2041 acgagatcat cgcgcgcggc ggccgctggc tcgacgtgcg gctgccgagc gagca
//
LOCUS       DATDMP010000057         3058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1676120, whole genome shotgun sequence.
ACCESSION   DATDMP010000057 DATDMP010000000
VERSION     DATDMP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676120"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /gene="secD"
                     /locus_tag="VI195_01000"
     CDS             <1..1266
                     /gene="secD"
                     /locus_tag="VI195_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY6822988.1"
                     /translation="GLVVTTENLPAGPAIQAMLSTAQVRERQDYAIQQNITTLRNRVN
                     ELGVSEPIVQRQGIDRINVQLPGVQNSAEVKDILGRVATLEFRLEDMQNSAYEAQQLG
                     RAPLGSKLYTHTRIGRPILLKREVIATGDELTNATAAQSQEGPAVNVRLDARAGANML
                     KTTRANLNKRMAVVLIEKHPETTEVNGQKVTREVTDEEVINDATIRGVFSTNFQITGL
                     QAGEARELALLLRSGSLAAPIYEIEERAVGPSLGRDNIDKGVTALIVGMAGVFVFMAI
                     YYKVFGLVADLVLLANVVLLAALLSMMRASLSLPGIAGIILTVGMAVDANVLIYERIR
                     EEIRKGVSPQAAIRAGFEKAFSAIADSNITTLIAGVVLWVLGTGPIRGFAIVLTLGIA
                     TSMFTALMGSRALLTLMYGGRRRLARLSI"
     gene            1301..2236
                     /gene="secF"
                     /locus_tag="VI195_01005"
     CDS             1301..2236
                     /gene="secF"
                     /locus_tag="VI195_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY6822989.1"
                     /translation="MEFFSHQTAYPFMATRKVWYTLSAVLMIVSLASFFVRGVNLAID
                     FTGGVSAEANFPQAVDVDRVRTQLAAAAFREPQVENFGSSRDIAIRLPPEGKQSASEV
                     RSRLETALRGVDARAQVVQLDVVGPQVGNELKMSAIWALFFTLLGIFLYIAFRFHTWR
                     LSLGAILAVMHDPILVLGFFSITQMSFDLAVVAAILAVIGYSLNDTVVVFDRIRERFE
                     GNRRSPPDVVLDQSINQTLSRTIMTKVVTSIVVVALLLLGGPVLKGFSAALLIGIIAG
                     TYSSIYISSAIALDFGLTAEHLFPTVKKAAFDQLP"
     gene            2297..2968
                     /locus_tag="VI195_01010"
     CDS             2297..2968
                     /locus_tag="VI195_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY6822990.1"
                     /translation="MLEIVLGIDNVIFISLLVERLPRARQARARSVGLALAMLTRIGL
                     LFSVVWLTHLTQPWLRLGAAAFSGRDLVLLGGGLFLLAKSVLEIHHTLEGAAAAAPLR
                     PLRGRLAAIVVQIALIDIVFSLDSVFTAVGLVRELPIMVAAIVVAILVMMGMARSIGA
                     LIARHPTIKMLALAFLILIGVALIASGLHFEIPRGYIYFAMAFSVVVELINLRLRRRL
                     DERRR"
BASE COUNT          441 a         1066 c         1028 g          523 t
ORIGIN      
        1 ggcctggtgg tcaccaccga gaatctgccg gccggacccg ccatccaggc gatgctgagc
       61 acggcgcagg tccgcgagcg ccaggactac gccatccagc agaacatcac cacgctgcgc
      121 aaccgggtga acgagctcgg cgtgtccgag cccatcgtgc agcgccaggg catcgaccgc
      181 atcaacgtgc agctgcccgg cgtgcagaac tccgccgaag tgaaggacat cctcggccgg
      241 gtggcgacgc tcgagttccg gctcgaggac atgcagaaca gcgcctacga ggcccagcag
      301 ctcggccggg cgccgctcgg ctccaagctc tacacccata cgcgcatcgg ccggcccatc
      361 ctgctgaagc gcgaagtcat cgccacgggc gatgagctca ccaacgcgac cgcggcgcag
      421 tcgcaggagg gtccggcggt caacgtgcgg ctcgatgcgc gcgccggcgc caacatgctc
      481 aagaccacgc gcgcgaatct caacaagcgc atggcggtgg tgctgatcga gaaacacccc
      541 gagaccaccg aggtcaacgg ccagaaggtg acccgcgagg tgaccgacga ggaggtcatc
      601 aacgacgcca ccatccgcgg ggtgttcagc accaacttcc agatcaccgg gctgcaggcc
      661 ggcgaggcgc gcgagctggc gctgctgctg cgctcgggct cgctcgccgc accgatctac
      721 gagatcgagg agcgtgcggt gggaccgagc ctcggccgcg acaacatcga caagggcgtg
      781 accgcgctca tcgtcggcat ggcgggcgtg ttcgtgttca tggcgatcta ctacaaggtc
      841 ttcggcctgg tggcggacct ggtgctgctc gccaacgtgg tgctgctcgc ggcgctgctg
      901 tcgatgatgc gcgcctcgct gtcgctgccg ggcatcgccg gcatcatcct caccgtcggc
      961 atggcggtcg acgccaacgt gctgatctac gagcgcatcc gcgaggaaat ccgcaagggc
     1021 gtctcccccc aggccgcgat ccgcgccggc ttcgagaagg ccttctcggc gatcgccgac
     1081 tccaacatca ccacgctgat cgccggggtg gtgctgtggg tgctcggcac cggcccgatc
     1141 cgcgggttcg cgatcgtgct cacgctcggc atcgccacct cgatgttcac tgcgctgatg
     1201 ggcagccgcg cgctcctcac gctcatgtac ggcggccgcc gcaggctcgc gcgcctgtcg
     1261 atctgagctg gcacgcagcg catagcccag gattcatacc gtggaattct tcagtcacca
     1321 gaccgcttac cccttcatgg ccacccgcaa ggtgtggtac acgctgtcgg cggtgctgat
     1381 gatcgtgtcg ctcgcttcct tcttcgtgcg cggcgtgaat ctcgccatcg atttcaccgg
     1441 cggggtgagc gcggaggcga actttcccca ggcggtcgac gtcgaccggg tgcgcaccca
     1501 gctcgccgcc gccgcgttcc gcgagccgca ggtggagaac ttcggctcct cgcgcgacat
     1561 cgccatccgc ctgccgcccg agggcaagca gtcggcgagc gaggtgcgct cgcgcctcga
     1621 gacggcactg cgcggcgtgg atgcccgcgc gcaggtggtg cagctcgatg tggtcggccc
     1681 gcaggtcggc aacgagctga agatgagcgc catctgggcg ctgttcttca cgctgctcgg
     1741 gatattcctc tacatcgcct tccgcttcca tacctggcgg ctgtcgctcg gcgcgatcct
     1801 cgccgtcatg cacgatccga tactggtgct cgggttcttc tcgatcaccc agatgtcgtt
     1861 cgatctggcg gtggtggcgg cgatcctggc ggtgatcggc tactcgctca acgacaccgt
     1921 ggtggtgttc gaccgcatcc gcgagcgctt cgagggcaac cgccgctcgc cgcccgacgt
     1981 ggtgctcgat cagtcgatca accagacgct gtcgcgcacc atcatgacca aggtggtgac
     2041 ctcgatcgtg gtggtggcgc tgctgctgct cggcgggccg gtcctcaagg gcttctccgc
     2101 cgcgctgctg atcggcatca tcgccggcac ctattcctcg atctacatct cgagcgccat
     2161 tgcgctcgac ttcggcctca cggccgagca cctgttcccg acggtcaaga aggcggcctt
     2221 cgatcagctg ccgtagcgga gcgcccaagc gctgatcctg gatccgcaca cctggctggc
     2281 gttcctcgcg ctgtcgctgc tcgagatcgt tctcggcatc gacaacgtga tcttcatctc
     2341 gctgctggtg gagcggctgc cgcgcgcgcg gcaggcgcgc gcccgcagcg tgggcctcgc
     2401 gctcgcgatg ctcacccgca tcgggctgct gttctcggtg gtgtggctca cccacctcac
     2461 gcagccgtgg ctgcgcctgg gcgccgcggc gttctcgggc cgcgacctgg tgctgctcgg
     2521 cggcggcctg ttcctgctcg ccaagagcgt gctcgagatc caccacacgc tcgagggcgc
     2581 cgccgcggcc gcgccgctac ggccgctgcg cggcaggctg gcggcgatcg tggtgcagat
     2641 cgcactcatc gacatcgtgt tctcgctgga ctcggtattc accgccgtgg gtctggtgcg
     2701 ggagctgccg atcatggtgg ccgccatcgt ggtggcgatc ctggtgatga tggggatggc
     2761 gcgatcgatc ggcgcgctga tcgcgcgcca ccccacgatc aagatgctgg cgctcgcgtt
     2821 cctcatcctg atcggcgtcg cgctgatcgc cagcggactg cacttcgaga tccccagggg
     2881 ctacatctac ttcgcgatgg cgttctcggt ggtggtggag ctgatcaacc tgcggctgcg
     2941 gcggcgcctg gacgagcgcc ggcgctgacg ccgcgccgcg cgcggcgcgg cggccggtca
     3001 gccgcgggtg cggggttcag cggatcgcgg cgggactcgg gcgcgcgccg gcgccgtc
//
LOCUS       DATDMP010000058         3076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_676424, whole genome shotgun sequence.
ACCESSION   DATDMP010000058 DATDMP010000000
VERSION     DATDMP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676424"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VI195_01015"
     CDS             complement(<1..340)
                     /locus_tag="VI195_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY6822991.1"
                     /translation="MAEGDGSIALLAGASGLTGSFTLDALLDAPDVSRVIAVTRRPLG
                     REHPRLANRIVQFERLESQLKGTTCDVALCCLGTTLRKAGSQQGFRAVDIDGVLTFAR
                     TAKAANARRFV"
     gene            complement(444..1976)
                     /locus_tag="VI195_01020"
     CDS             complement(444..1976)
                     /locus_tag="VI195_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6822992.1"
                     /translation="MAGTEVLICGAGPTGLVLALWLTRQGVGVRIIDRSAAPGTTSRA
                     LAVQARTLELYRQLDLTQEVLERGNSVPAVNLWSRGERAARVAFGQICADLTPYPFLE
                     IYPQDEHERLLIARLQQLGVTVERRTELTAFTDAGDHVRAQLRTADGTEMSGEFRYLA
                     GCDGARSMVREALGTGFPGGTYRHVFYVADIVGAGAPIDGELHVDLDEADFLAIFPLA
                     AAGHARLVGTVQDERAAHAETLRFEDVSQRAIQNLRLVVNKVNWFSTYHVHHRVTGHF
                     SRERVFLLGDAAHVHSPVGGQGMNTGIGDAINLAWKLAAVLQGRADERLLATYEAERI
                     GFAQRLVATTDRVFTFATAQGRIADLIRTRLAPALLARVLSLESVREYLFRTVSQIMV
                     NYRGSALSAGAVGEVHGGDRLPWVAHDGRDNYATLAAIAWQVHVYGEASAALREASAA
                     LGVALESFPWSAAHAAAGLERNALYLLRPDGYVALADRAADPARLTHYFSARALRPQA
                     AH"
     gene            complement(1998..>3076)
                     /locus_tag="VI195_01025"
     CDS             complement(1998..>3076)
                     /locus_tag="VI195_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822993.1"
                     /translation="TQLAAKRTEFDQRFAFLEARGAASWAAAELAKARRLAAESVGAR
                     DAGSMALAQQRLSEASRVLDSIEQAAPGGANAVAASTAQAQVGPAVTTPPAAPGDSGT
                     TQSGSAPPVVAGAAKPPASGAFADDSYAKAAGEGFAALGAGQLDKAQRAFEKARALRP
                     NGSEALAGLRRVEAARSTRTLSVRRAEAEDLEDEERWQDALDAYYAVLRQDSSLGWAQ
                     EGQARAGARLQLGDSLQALIDHPERLSNPRLRDEAAALLQTAAQQTSSGPVLRTQVTR
                     LTGLLPTLDKPVRLSLVSDSRTQVSIPSVGSFGSFARHDIELKPGRYTVIGTRDGYRE
                     VRRDITVSPGQEYLTVNVSCSEPI"
BASE COUNT          501 a         1071 c         1042 g          462 t
ORIGIN      
        1 cgacgaagcg gcgcgcattc gccgccttgg cggtacgggc gaaggtcagc acgccatcga
       61 tatcgaccgc gcgaaagcct tgctgcgagc cggctttgcg cagcgtggtg ccgaggcagc
      121 acaaagcgac atcgcaggta gtgcccttga gctgcgactc gaggcgctcg aattgcacga
      181 tacgattggc gaggcgtgga tgctcgcgcc cgagcgggcg ccgcgtcacg gcgatcacgc
      241 ggctcacatc gggcgcatcg agcagcgcat cgagcgtgaa gctcccggtc aaaccgctcg
      301 cgccggcgag cagcgcaatg ctgccatccc cttctgccat cgccgcaccg ccgttgacag
      361 atgttgtttg agcgcagctt agaagcgcgc gctgcccgcg gcaagcgcga ttgcggggcc
      421 gctggcacgc gtgtcggcga gcgtcaatgc gctgcctggg gacgcagcgc gcgcgcgctg
      481 aagtaatgcg tgagccgggc aggatccgcc gcgcgatccg cgagcgctac gtaaccgtcc
      541 gggcgcagca gataaagcgc attgcgctcg aggccggccg ccgcgtgcgc cgcgctccag
      601 ggaaacgact cgagcgctac accgagcgcc gcgctcgcct cgcgcaaggc cgctgaggcc
      661 tcgccataga cgtgcacctg ccaggcgatt gccgcgagcg tggcgtaatt gtcgcggccg
      721 tcgtgcgcca cccatggcaa gcggtcgccg ccgtggacct cacccacggc accggcgctc
      781 agcgcggaac cgcggtagtt caccatgatc tgcgacaccg tgcgaaacag gtactcgcgc
      841 accgactcga gcgagagcac ccgggccagc agcgccggcg cgaggcgcgt gcgtatcaga
      901 tcggcgatcc tgccctgcgc ggtagcgaaa gtgaacacgc gatcggtcgt cgccaccagt
      961 cgctgcgcga agccgatgcg ctcggcttcg taggtcgcaa gcagccgctc atctgcgcgc
     1021 ccctgaagca ccgcggcgag cttccaggcg agattgatgg catcgccaat gccggtattc
     1081 atcccctgtc cgccgaccgg gctgtgcacg tgcgcggcat cgccgagcag gaatacccgc
     1141 tcgcgactga agtggccggt cacgcggtgg tggacatggt aggtggagaa ccagttcacc
     1201 ttgttcacca cgagccggag gttctggatg gcgcgctggc tgacgtcctc gaagcgcaga
     1261 gtctccgcat gggcagcgcg ttcgtcctgg accgtaccga cgagtcttgc gtgccccgcg
     1321 gcggcgagcg ggaagatcgc gagaaaatcc gcttcgtcga gatcgacatg cagctcgcca
     1381 tcgatgggcg cgcccgcgcc cacgatatcg gcgacgtaga acacgtggcg gtaggttccc
     1441 ccgggaaagc cagtgccgag cgcctcgcgc accatggaac gcgcgccatc gcaaccggcg
     1501 aggtagcgaa actcgccgct catctctgtg ccatcggccg tgcgcagctg cgcacgcacg
     1561 tgatcgcccg catctgtgaa ggcggtgagc tcggtgcgcc gctcgaccgt tacaccaagc
     1621 tgctgcaggc gcgcgatgag cagccgctcg tgctcgtcct gcggatagat ctcgagaaag
     1681 ggatagggtg tcaggtccgc gcagatttgc ccgaaggcga cgcgcgccgc gcgctcgccg
     1741 cgcgaccaca gattgaccgc aggcacgctg ttaccgcgct cgagcacctc ctgagtcaga
     1801 tcgagctgcc ggtagagctc gagcgtgcgc gcctgcaccg cgagcgcccg cgatgtggtg
     1861 ccgggcgcag cgctgcggtc gatgatgcgc acacccacac cctgacgggt cagccacagc
     1921 gccagcacca gcccggtggg acctgcgcca cagatgagaa cctcggtgcc tgccatcgct
     1981 cggcctgtcg cggtggctca gatcggctcc gagcagctga cattgacggt cagatattcc
     2041 tggccggggc tgaccgtgat gtcgcggcgt acctcgcggt acccatcgcg cgtaccgatc
     2101 acggtgtagc gcccgggctt gagctcgatg tcgtgccggg cgaagctgcc gaagctgccg
     2161 acgctcggaa tcgacacttg cgtgcggctg tccgatacca gcgagagacg caccggctta
     2221 tccagcgtgg gcagtaaccc ggtgagccgt gtgacctgcg tacgcaacac cgggcccgag
     2281 ctggtctgct gcgcggcagt ctgcaacagt gccgcggctt catcgcgcag ccgtggattc
     2341 gacagccgct cgggatggtc gatcagcgcc tgcagcgagt caccgagctg caaacgcgcg
     2401 ccggcgcgtg cctgcccctc ctgcgcccaa ccgagcgagc tatcctggcg cagcacggca
     2461 tagtacgcat cgagcgcatc ctgccagcgc tcctcatcct cgaggtcctc agcctcggcg
     2521 cgacgcaccg agagcgtgcg cgtcgaccgc gcagcttcga cacgccgcag gccagccagc
     2581 gcttcggacc cattggggcg cagcgcgcgc gccttctcga acgcacgctg ggccttatcg
     2641 agttgccccg ctccgagagc cgcgaatccc tcacccgccg ccttagcgta cgagtcatcc
     2701 gcaaaggctc cgctggccgg aggtttcgcc gcgccagcga ccactggggg cgcacttccc
     2761 gattgagtcg tgcccgagtc gcctggcgct gccggcgggg ttgtcaccgc gggccccacc
     2821 tgggcctgcg cggtagaggc agcgaccgca ttcgcaccgc cgggggcggc ttgctcgatg
     2881 gaatccaaca cgcgcgaggc ttcggagaga cgctgctgcg cgagcgccat actgccggca
     2941 tcgcgcgcgc ccaccgattc cgcggcgagc ctgcgggctt tggcgagctc cgctgcggcc
     3001 cagctcgctg cgccgcgcgc ttcgagaaag gcgaagcgct ggtcgaattc cgttcgcttg
     3061 gccgcgagtt gcgtga
//
LOCUS       DATDMP010000059         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1232224, whole genome shotgun sequence.
ACCESSION   DATDMP010000059 DATDMP010000000
VERSION     DATDMP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232224"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VI195_01030"
     CDS             <1..1255
                     /locus_tag="VI195_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY6822994.1"
                     /translation="HTEPASAGSIIGGVDTTMDGLADTFSRPGPRPTVGLVLTGGGAR
                     SAYQVGVLLALAEMLPRARNPFQVIVGTSAGAVAASVLAAEAHHWRRAVAGLERVWAN
                     FHSSQVFHVDATHMMRAGAHWVLALVSGGMVLSPPKSMLDNSPLRQLLAVHVDCAGIR
                     RSIERGHVRAFALCATSYSNGHSVAFFDGIDSIRDWSRVQRVGVRTPLSLDHLMASAA
                     IPLLFPPIRIGDEYYGDGAMRQLHPLSPAIHLGADRLLIVGVRARRAAGVQVSRLPAI
                     MPTPGEIFGYMLDTLFTDQIYGDLEQLERFNELVHAAPQVARGGRPIETLMLAPSVDP
                     RELAARHAGEIPRGLRALLRVIAGRDASGYQLASYLTFESGYTRALIELGYRDAMEAR
                     SALMAFMSGEKLPQVMTAAGVAQAT"
     gene            complement(1389..2291)
                     /gene="rapZ"
                     /locus_tag="VI195_01035"
     CDS             complement(1389..2291)
                     /gene="rapZ"
                     /locus_tag="VI195_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY6822995.1"
                     /translation="MRIIILSGLSGSGKSVALHMLEDLGFYCIDNIPAALLKPFVSYT
                     VRSPEATYERTAIGLDARNTPAEVASVPQLIDELRRSGLQCEVVFLVAAEDELLRRFA
                     ETRRKHPMSRSGIGLREAMALERELLEPIASVADLLIDTSRLSVHSLRDIIHKRVEQR
                     SAGKLSLTFESFGFKRGIPADADFVFDARALPNPYWEPGLRNLTGRDAEVIRFLETHT
                     NVAALVADIARFVRGRVPEYQASNRGYLTVAVGCTGGQHRSVYIVDRLAEIFAAESAN
                     VMARHSGLPGAKLFKPVRAAAAAP"
     gene            complement(2358..2675)
                     /gene="raiA"
                     /locus_tag="VI195_01040"
     CDS             complement(2358..2675)
                     /gene="raiA"
                     /locus_tag="VI195_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY6822996.1"
                     /translation="MQLSVTGHHVEVTAALRGYVEKKLERIGRHFDQVIDIHCVLTVE
                     KLRQKAEATLHVSGNAIHADATQPDMYAAIDLLADKLDRCIKKHKEKRADHHAAAVRA
                     LRL"
BASE COUNT          424 a          953 c          903 g          478 t
ORIGIN      
        1 tcacacagag cccgccagcg ccggttccat aataggcggc gttgacacca ctatggatgg
       61 ccttgccgat accttctccc gacccggccc gcgccccacc gtcggcctgg ttctgacagg
      121 cgggggcgcg cgctcggcct atcaggtcgg agtgttgctc gccctcgccg agatgctgcc
      181 gcgcgcgcgc aatcccttcc aggtgatcgt aggcacctcc gccggtgcgg tcgctgccag
      241 cgtgctcgcg gccgaggcgc accactggcg gcgcgcggtg gcgggcctcg agcgggtgtg
      301 ggccaacttc cactcgagcc aggtattcca cgtggatgcg acgcacatga tgcgcgcggg
      361 cgcccactgg gtgctggcac tcgtctccgg cggcatggtg ctgtcgccgc caaagtcgat
      421 gctcgataac tcgccactgc ggcagctact cgccgtgcac gtcgactgcg ccggcatccg
      481 ccgcagcatc gagcgcgggc acgtgcgcgc cttcgcactg tgcgccacca gctacagcaa
      541 cggtcactcg gtggcgttct tcgacggcat cgactcgatc cgcgactggt cacgcgtgca
      601 gcgcgtcggc gtgcgcaccc cgctcagtct ggatcacttg atggcgagcg ccgcgatccc
      661 gctgctgttt ccgccgatca ggatcggcga tgaatactac ggcgatggcg ccatgcgcca
      721 gctgcatccg ctcagccccg cgatccatct cggcgccgac cggctgctga tcgtcggcgt
      781 gcgcgcgcgc cgcgccgccg gtgtgcaggt gagccgcctg ccggcgatca tgccgacgcc
      841 cggagagatc ttcggctaca tgctcgatac gctgttcacc gaccagatct acggcgacct
      901 cgagcagctc gagcgcttca acgagctggt gcatgcggca ccacaggtcg cgcgcggtgg
      961 ccgccccata gagacgctca tgctggcacc ctcggtggat ccgcgcgagc tcgccgcgcg
     1021 ccatgcgggg gagattcccc gtggcctgcg cgcactgctg cgcgtcatcg ccggccgcga
     1081 cgcctccggc taccagctcg ccagctatct gaccttcgag tccggctaca cgcgcgcgct
     1141 catcgagctc ggctaccgcg acgccatgga agcgcgcagc gcgctcatgg cattcatgag
     1201 cggggagaag ctgccgcagg tcatgaccgc ggccggggtc gcgcaggcga cttgaagccg
     1261 ccgcggcggc cgtgatccgg ctgcgtgctc atatctcgtc ctgaggttag ttaaccagat
     1321 ggttaactat tgctgaaaag agctccaact ctctggcgct cgccccccaa ctctctggcg
     1381 ctcgccgttt acggggcggc agcggcggcg cgcaccggct tgaacagctt ggctccgggc
     1441 agcccgctgt ggcgcgccat cacattggca gattcggctg cgaagatctc cgccaggcgg
     1501 tcgacgatgt acaccgagcg atgctgaccg ccggtgcagc cgaccgccac cgtgaggtag
     1561 ccgcggttgc tcgcctggta ctccggcacc cggccgcgca cgaagcgggc gatgtccgca
     1621 accagcgccg cgacgttggt gtgcgtctcg aggaagcgga tcacctcggc atcgcgaccg
     1681 gtgagattgc ggaggcccgg ctcccagtag ggattgggca gggcgcgcgc atcgaatacg
     1741 aagtcggcgt cggcaggtat gccgcgcttg aagccgaagg actcgaaggt gagcgagagc
     1801 ttgccggcgg agcgctgctc gacgcgcttg tggatgatgt cgcgcagcga gtgcaccgac
     1861 aggcgcgagg tgtcgatgag cagatcggct accgaggcga tcggctcgag cagctcgcgc
     1921 tcgagcgcca tcgcttcacg caaaccgatg ccgctgcggc tcatgggatg cttgcggcgc
     1981 gtttccgcga aacgccgcag cagctcatcc tccgctgcga ccaggaacac gacctcgcac
     2041 tgcaggccgc tgcggcgcag ctcatcgatc agctgcggaa cgctcgccac ttcggccgga
     2101 gtattgcgcg catcgagccc gatcgccgtg cgctcataag tcgcctccgg gctgcgcacg
     2161 gtataggaaa cgaacggctt cagcagcgcc gcggggatgt tatcgatgca gtaaaacccc
     2221 aggtcctcga gcatgtgcaa ggccacgctc ttgccggacc ccgacagccc gctgaggatg
     2281 atgatgcgca cgccgccatg gtaaccagcg gcagcgccga ggcaagtttc cgctccggga
     2341 ggcccgcttc ggccccccta gagccgcagg gcgcgcaccg cggcggcgtg gtggtcggcg
     2401 cgcttctcct tgtgcttctt gatgcagcga tctagcttgt ccgcgagcag atcaatggcc
     2461 gcgtacatgt ccggctgggt ggcatccgca tggatcgcat tgccgctcac gtgcagcgtc
     2521 gcctcggcct tctgtcgcag cttctccacc gtcaacacgc aatgtatgtc gataacctga
     2581 tcgaaatgcc tgccgatgcg ctccagtttc ttttccacat agccccgcag tgcggcggtg
     2641 acttcgacgt ggtggccggt aacgctgagc tgcatacgtc gtctccttga ttgatttctc
     2701 gcttacatct gtttcggccc cgcgcggcgg cgctcgttcg aggccgcgat tcccatgg
//
LOCUS       DATDMP010000060         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_565793, whole genome shotgun sequence.
ACCESSION   DATDMP010000060 DATDMP010000000
VERSION     DATDMP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565793"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VI195_01045"
     CDS             complement(<1..684)
                     /locus_tag="VI195_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6822997.1"
                     /translation="MPTRALQRIIEAVPTSDGAGVKLRRSLGQSQDARLDPFLMLDHF
                     STDKADDYIAGFPPHPHRGFETVTYMLDGHMQHKDHLGNTGDLRSGAVQWMTAGRGII
                     HSEMPQQEQGRMRGFQLWINLPAKEKMKPAAYRDIQPGEIPAVDLEGGGRVRVIAGEP
                     TLDGRKVPGPIQGSTTDPLYLDVELPAGARFAQAIPAGYNAFVYPFEGSVQVGEDRAR
                     PLNTHDAGVL"
     gene            806..1861
                     /locus_tag="VI195_01050"
     CDS             806..1861
                     /locus_tag="VI195_01050"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6822998.1"
                     /translation="MKRLLNQVFPAAVALACALFAHPALATFHTYQIFSIYSNADGTV
                     QYLVLHESLGMNGQSSFHGHVLTSSSAGSNQMYVFPSDLPGGEPGDGYGMGPMLAPTA
                     NTYVLIATQGFADLNLVTPDYVVPNGFIPLTNGTVNYAGVDQVSYAALPTDGTSAINR
                     NGTSIPNMATNFAGSAAAVAPAAPQPPGTVVAVEYYYAAWNFYFETSFPDEIAGLDAG
                     AFGGVWKRTGQIFYVWPSAAASPLGVATCRFFTVFFAPKSSHFYTPNADECQGLIATS
                     QVWQFESIAFYIALADANGNCPPGTIPLYRFYDNGMGGAPNHRYTTSLTVFNQMVAQG
                     WQFEGNPVTKVFACVPG"
     gene            complement(1908..>2171)
                     /locus_tag="VI195_01055"
     CDS             complement(1908..>2171)
                     /locus_tag="VI195_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY6822999.1"
                     /translation="LSRDHVIPVSRGGRDVWTNSVTACRRCNTKKANRSPEAADMPLL
                     YVPYVPNRHEHFILRNRRILADQMEYLLAGVPRTSRLYVSPLS"
BASE COUNT          348 a          708 c          706 g          409 t
ORIGIN      
        1 gagcacgccc gcgtcgtgcg tgttcagcgg ccgcgcgcga tcctcgccga cttgcacgct
       61 gccctcgaac ggatagacga aggcgttgta gcctgcggga atcgcctgcg cgaatctcgc
      121 gcccgcgggc agctcgacgt cgaggtaaag cggatcggtc gtcgagccct ggatcggtcc
      181 cggcaccttc cggccgtcca gcgtcggctc gccggcgatg accctgacgc gccctccgcc
      241 ctcgagatcg actgcaggga tttctccggg ttggatgtcg cggtatgccg cgggcttcat
      301 cttctccttc gccggcaggt tgatccagag ctggaagccg cgcatccggc cctgctcctg
      361 ttgcggcatc tcggagtgga tgatgccgcg tcccgcggtc atccattgca ctgcgccgct
      421 gcgcagatca cccgtgttcc cgagatggtc tttgtgttgc atgtgaccgt cgagcatgta
      481 ggtgaccgtc tcgaagccgc gatgcggatg cggcggaaat ccggcgatat agtcgtcagc
      541 cttgtcggtg gagaagtggt cgagcatcag gaaggggtcg aggcgcgcat cctgcgactg
      601 tcccaggctg cggcgcagct tgactcctgc gccgtcggag gtcgggaccg cttcgatgat
      661 gcgttgcagg gctcgggtag gcataaactg caatgtgtgg ccgatgcatg gggacgtcaa
      721 cccccgagcg ggaataccct cgtggcggcg agtgtttgcc ggtcgcagac tctggccttg
      781 cggcctcgat ttcgcggagc acgccatgaa acggcttctg aaccaggtct ttcccgcggc
      841 ggtcgcgctc gcatgcgcgc tcttcgccca tcccgcgctg gcgacgtttc acacgtatca
      901 gatcttcagc atctactcca acgcggacgg gacggtgcag tacctcgtgc tgcacgagtc
      961 gctcggcatg aatggccaga gttcgttcca cggtcatgtg ctgaccagct cctctgccgg
     1021 aagcaatcag atgtacgtgt ttccgagcga tctgcccggc ggcgagcctg gagacggcta
     1081 cggcatgggg ccgatgctcg ccccgaccgc caacacctac gtgctcatcg cgactcaggg
     1141 gtttgccgac ctgaatctcg tcacgccgga ctacgtggtg ccgaacggct tcattccgct
     1201 gacgaacggc acggtcaatt acgccggcgt cgatcaggtg agctacgcag cgctgccgac
     1261 cgacggcacg agcgcgatca accgcaacgg caccagcatt ccgaacatgg cgaccaactt
     1321 tgccggcagc gcggcggccg tggcgcccgc tgcaccgcaa cctcccggga cggtcgtcgc
     1381 ggtcgagtac tactacgccg cctggaattt ctacttcgag acttccttcc cggacgagat
     1441 cgcgggcctc gacgccggcg ccttcggcgg cgtgtggaag cgtaccggcc agatcttcta
     1501 cgtgtggcct tcggccgccg ccagtccgct gggggtggcg acctgccgct tcttcaccgt
     1561 cttcttcgcc ccgaagagct cgcacttcta cacgccgaac gccgacgaat gccaagggct
     1621 cattgccacg agccaggtct ggcagttcga gtcgatcgcc ttttacatcg cactggccga
     1681 cgcgaatggc aattgccctc cgggcaccat tccgctctac cgcttctacg ataacggcat
     1741 gggcggagcg cccaatcacc gttacaccac gagcctgacg gtgttcaatc agatggtcgc
     1801 ccagggctgg caattcgagg gcaacccggt gaccaaggtg ttcgcctgcg tgcccggctg
     1861 atcgaggcac gcttcgagcg gcttgcgggc cctgctgcgg gattcggcta ggacagcgga
     1921 gagacgtaga gccggctggt gcggggcacg ccggccagca ggtattccat ctggtcggcg
     1981 aggatccggc ggttgcgcag gatgaaatgc tcgtgccggt tgggcacgta cggcacgtac
     2041 agcaatggca tgtcggcggc ttccggcgag cgattcgcct tcttggtgtt gcagcgccgg
     2101 caggcggtga ccgagttggt ccatacatcg cggcctccgc gcgacaccgg gatcacgtga
     2161 tcgcgcgaca g
//
LOCUS       DATDMP010000061         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_565924, whole genome shotgun sequence.
ACCESSION   DATDMP010000061 DATDMP010000000
VERSION     DATDMP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565924"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="hemA"
                     /locus_tag="VI195_01060"
     CDS             complement(<1..657)
                     /gene="hemA"
                     /locus_tag="VI195_01060"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY6823000.1"
                     /translation="MHLFAFGINHASAPLSVREQVVFHAERLVEALRELVDRKPVKEA
                     AIISTCNRTEVYCNTEEPRAAMEWMAGYHSLKPQQIEPYLYLLPQERAVKHAFRVASG
                     LDSMVLGEPQILGQFKQAVRTAQAAGTLGLVLNKLFQRTFAVAKTVRSETEIGAAKVS
                     MASAAVGLAQRIYPSISGQNVLFIGAGEMIELCATHFAAQHPRRLTFANRTLERAQAL
                     A"
     gene            complement(770..1762)
                     /locus_tag="VI195_01065"
     CDS             complement(770..1762)
                     /locus_tag="VI195_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY6823001.1"
                     /translation="MTGILLSKGTFTAYREPLARAARAAGIEPRVIHLPDDPEARFTP
                     QESAAIEIAFFTRDMRFSNQYQSFVDTVVAAPNLKWVHFPNAGVDQHPFLPALAARKV
                     RLTTSAGSNGEPVGQTAIAGLLLLARNFPHWWAAQGRREWKPIRGDSVPRDLRGQTVL
                     IVGMGTVGTTVARFCRVLGMHVIGMRRKPRSPDDPVDEMHTLAALRELLPRCDWVVLA
                     CSLTPETRRIINTETLALLPRGARLVNVCRGGVVDEAAVIEALKSGQLGGAYLDVFEN
                     EPLPPESPLWGLPNVIVSPHNAQASSGNDGRAMDIFLGNLVQWARGERMRNEES"
     gene            complement(1851..>2138)
                     /gene="prpB"
                     /locus_tag="VI195_01070"
     CDS             complement(1851..>2138)
                     /gene="prpB"
                     /locus_tag="VI195_01070"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142557.1"
                     /note="catalyzes the formation of pyruvate and succinate
                     from 2-methylisocitrate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY6823002.1"
                     /translation="VKVPILANITEFGKTPLFTAKELAGANVAMALYPLSAFRAMNAA
                     ALRVYEGIRKDGSQKGVVELMQSRDELYDYLGYHDYESKLDALFAREKKKK"
BASE COUNT          374 a          686 c          720 g          358 t
ORIGIN      
        1 ggccagcgcc tgcgcgcgct cgagcgtgcg gttggcgaag gtgagcctgc gtggatgctg
       61 cgcggcgaaa tgggtggcgc agagctcgat catttcgccg gcgccgatga agagcacgtt
      121 ctgcccggag atcgagggat aaatgcgctg tgcgagcccg accgcagcgg acgccatcga
      181 caccttggcg gcgccgatct cggtctccga gcgcaccgtc ttcgcgaccg caaaggtgcg
      241 ttgaaagagc ttgttgagga ccagcccaag cgtgcccgcg gcctgcgcgg tgcgcaccgc
      301 ctgcttgaac tgtcccagga tctgcggctc gccgagcacc atggaatcga gtccgctcgc
      361 cacccggaag gcgtgcttca ccgcccgctc ctgcggcaac aggtagagat agggctcgat
      421 ctgctgcggc ttcaggctat ggtagccggc catccactcc atcgcggcgc gcggttcctc
      481 cgtattgcag tagacctcgg tacggttgca ggtcgagatg atcgccgctt ccttcaccgg
      541 cttgcggtcc accagctcgc gcagcgcctc caccaggcgc tcggcgtgaa acaccacctg
      601 ctcgcgcacc gatagcggcg cggaggcgtg gttgatgccg aaggcgaaca ggtgcatggg
      661 gcgcaagagc ctgaaaaatt ggtcgaatat tatagaacta tcccgtgccc gggggcaggt
      721 tggcgcgggg aaaaagccga acgcggaagg gcgcgccggc gaggcgaagc taggactctt
      781 cgttccgcat gcgctcgccc cgggcccatt gcacgagatt gccgaggaag atgtccatgg
      841 cgcgaccgtc gttgccggag gaggcctggg cgttgtgcgg cgagacgatg acgttgggca
      901 ggccccacag cggagattcc ggcgggagcg gctcgttctc gaagacatcg agataggcgc
      961 cgcccagctg accgctcttc agcgcctcga tcaccgcggc ctcgtccacc acgccgccgc
     1021 ggcaaacatt gaccagccgc gcgccgcgcg gaagcagcgc cagtgtctcc gtgttgatga
     1081 tgcggcgcgt ttccggggtc agcgagcagg ccagcaccac ccagtcacag cgtggaagca
     1141 gctcgcgcag ggcggccagc gtatgcatct cgtcgaccgg gtcatccggg ctcctcggct
     1201 tgcgccgcat gccaatgacg tgcatgccga gcacgcggca gaagcgcgcc accgtggtgc
     1261 ccacggtgcc catgccgacg atcagcacgg tctgcccgcg caggtcgcgc ggcacgctgt
     1321 cgcctcgaat cggtttccac tcgcggcggc cctgcgccgc ccaccagtgc gggaaattgc
     1381 gcgcgagaag cagcagcccc gcgatcgccg tctgtcccac cggctcgccg ttagacccgg
     1441 cggaggtcgt cagccgcacc ttgcgcgcag cgagcgccgg caggaacgga tgctggtcga
     1501 cgccggcgtt gggaaaatgc acccacttga gattgggcgc cgcgacgacg gtatcgacga
     1561 aagactggta ctgattcgag aaacgcatgt cgcgggtgaa aaaagcaatc tcgattgccg
     1621 cgctctcctg cggcgtgaaa cgcgcctccg gatcgtccgg cagatgaatc acgcgcggtt
     1681 cgattcccgc cgcgcgcgcc gcgcgcgcca gcggctctcg ataggccgtg aacgtgccct
     1741 tggaaagcag gattccggtc atggaaaaat cggtgacgac tgacgactaa tgactgacga
     1801 gaacggacga gggcggttta ttctcgccat cggtcgtcag tggtcagtcg ttacttcttc
     1861 tttttttcgc gggcgaaaag ggcgtcgagc ttgctttcgt agtcgtggta gccgaggtag
     1921 tcgtagagtt cgtcgcgaga ctgcatcagt tccaccacgc ccttctgcga gccgtccttg
     1981 cggatgccct cgtagacgcg cagcgcggcg gcattcatcg cgcggaacgc agacagcgga
     2041 tagagcgcca tggcgacgtt ggcgccggca agctccttcg cggtgaagag cggcgtcttg
     2101 ccgaactccg tgatgttggc gaggatcggc accttcac
//
LOCUS       DATDMP010000062         3033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1010370, whole genome shotgun sequence.
ACCESSION   DATDMP010000062 DATDMP010000000
VERSION     DATDMP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010370"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VI195_01075"
     CDS             complement(<1..749)
                     /locus_tag="VI195_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6823003.1"
                     /translation="MSTLSYALRDSATMLRRDARHSLRRLSMTLSGLGTPTIMLVLFN
                     YFFGGTLGAGLGGVAHGGAYINYVTPGILIMTVCSGCETTAINLCMDMSEGIIARFRT
                     MAISRASVLTGQVLGSLIRTLISVVLVAGVALLMGFRPTTNPVAWIAALGVIALFTFA
                     VTWMGVVFGLAGKTPAGANSLALIFLLLTFTSSAFVSTESMAGWVRWFAQYQPVTPVI
                     DTVRGLLLGTPIGNSAVLAVAWCAALALAGYL"
     gene            complement(746..1696)
                     /locus_tag="VI195_01080"
     CDS             complement(746..1696)
                     /locus_tag="VI195_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6823004.1"
                     /translation="MMIEARGLRKSFGDHVVLDGIDLDVAAGTIFALLGPNGAGKTTT
                     VQILSTLIRADGGEARVAGHDLGREPDAVRAVIGVTGQFSAVDNLLTGEENLLLMADL
                     HHLGRREGRRRAAKLLERFELADAAKKLPVTYSGGMRRRLDLAMTLMGDPRVIFLDEP
                     TTGLDPRSRHTMWQIIRDLVAGGVTILLTTQYLDEADELANRIAVLDRGRLVAEGTPD
                     ELKRRIPGGHILLRFAETGGLEAAARTLGEGSRDDDALTLQVPTDGSARSLRAVLDRL
                     DQEATGVVGVSVHTPDLDDVFFALTGHPDTVTATEKGAVR"
     gene            1779..2897
                     /locus_tag="VI195_01085"
     CDS             1779..2897
                     /locus_tag="VI195_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY6823005.1"
                     /translation="MYWEKSQMSRGSQENRLELLRELARELRQSSGLGASFFRAAAAR
                     IGMTDTDMQVIDSLDSAGPMTAGQLADLTGLTTGAITGMLNRLEEAGLVRRERDPNDG
                     RRVIVRLASDTDKMRETISIFDSARKVWDEIASPYDDEQIAFLLEFQQRANALFRSEI
                     VRLREAPEAREREFSAPLGGLRGGRLVIPAGIFQLSLRADAEMTALYQARFEGAVPDV
                     KTKDGEVTIRYPRRLWVLGGKMGAAAVTLNAAIPWQILIQGGASDITAELGNLDLAGL
                     EVKGGMSMIRLHLPVPSRTVPIYFSGGASEIIMRRPAGVAARVHLKGWASTFIFDDQT
                     FGNLGNDVRLQSPGYDTTAQRYDIEVASSASMVTITAE"
BASE COUNT          565 a         1015 c          971 g          482 t
ORIGIN      
        1 aggtatccag ccagggcgag cgcggcgcac caggcgacag caaggaccgc gctgttgccg
       61 attggggttc ccagcagcag ccctcgcacc gtatcaatca cgggcgtgac gggctggtac
      121 tgcgcgaacc agcgcaccca gcccgccatg gactccgtac tgacaaacgc gctgctggtg
      181 aacgtcagca gcaggaagat gagggcgagg ctattggctc ccgcgggcgt tttgcccgcc
      241 aggccgaaca ccacccccat ccacgtaacc gcgaaggtga acagcgcgat gacgccgagc
      301 gccgcgatcc aggcgacagg attcgtggtg ggccggaagc ccatcagcag cgcgactccg
      361 gcgaccagga cgacgctgat cagcgtccgg atcaggctgc cgagcacctg tccggtcagc
      421 accgaagcgc gagagatcgc catcgtgcgg aagcgggcga tgatgccttc gctcatgtcc
      481 atgcacaggt tgatcgcggt cgtctcgcac ccgctacaca ccgtcatgat gagaattccc
      541 ggcgtgacgt agttgatata ggcgccgccg tgcgccacac cgcccaggcc ggcgcccagc
      601 gtgccaccga agaagtagtt gaacagcacc agcatgatgg tcggtgtccc cagaccactc
      661 agcgtcatcg agaggcggcg cagcgagtgc ctggcgtcgc gccgcagcat cgtcgccgaa
      721 tcgcgcaggg cataggacag ggtgctcatc gtacggcgcc tttctctgta gcggtcactg
      781 tgtcagggtg gccggtgagg gcaaagaaaa cgtcgtcgag gtcgggggta tgcaccgaca
      841 ccccaacgac cccggtcgct tcttggtcga gccggtcgag cacggcccgc aaggaccggg
      901 cactgccgtc agttggaact tgcagcgtga gcgcatcgtc gtcacgcgac ccctcgccca
      961 gggtgcgagc ggccgcttcg agcccgcccg tctcggcgaa ccgcaggagg atgtggccgc
     1021 cggggatgcg gcgtttcagc tcgtcgggcg tcccctcggc aaccagcctc ccccggtcga
     1081 gcaccgcgat ccggttggcg agctcgtccg cctcgtccaa atactgcgtg gtgagcaaga
     1141 tcgtgacgcc gcccgctacc agatcgcgga tgatctgcca catcgtgtgg cggctgcgcg
     1201 gatccaggcc agtggtcggc tcgtcgagga agatgacacg cgggtcgccc atcaaggtca
     1261 tcgccaggtc gagccggcgc cgcatgccgc cggagtaggt gacgggcagc ttcttggccg
     1321 cgtcggccag ctcgaaacgc tcgagcagct tcgccgcgcg ccgccggccc tcgcgccggc
     1381 ccaggtggtg cagatcagcc atcaaaagca ggttctcttc cccggtgagc aggttgtcca
     1441 ccgccgagaa ctggccggtg acgccgatca cggcgcgcac cgcgtcaggc tcccggccca
     1501 gatcatgccc ggcgacccgc gcctcgccac cgtcggcacg gatgagcgtg gacagaatct
     1561 gcaccgtcgt ggtcttgccc gcgccattcg ggccgagcag cgcgaagatc gttcccgccg
     1621 ccacatccag gtcaatgccg tcgagcacca cgtggtcgcc gaacgacttg cgcaagcccc
     1681 gtgcttcgat catcatcacg atgcctccac atgctataat gggactcact gagccgcaaa
     1741 gtgaatcatg cgatataatc acttagtggc taaggcaaat gtactgggag aaatcacaaa
     1801 tgtcaagagg ttctcaggaa aaccgcctgg agctgctgag agagcttgcg cgcgagctcc
     1861 ggcagtcgag cggactcggc gcatcctttt tccgggcggc ggcggcgcgc atcggcatga
     1921 cggacacgga catgcaggtc attgacagtc tggatagcgc cggtcccatg accgccggtc
     1981 agctcgccga tctcaccggg ctgaccaccg gagccatcac tggcatgctc aaccgcttgg
     2041 aggaggccgg gcttgtgcgc cgcgagcgcg accccaacga cggccgcagg gtcatcgtcc
     2101 ggctcgcctc agatacggac aaaatgcgcg agaccatctc gatcttcgat tccgcccgca
     2161 aggtgtggga tgaaatagcg tctccctatg acgatgagca gatcgctttc cttctggagt
     2221 tccagcaacg cgccaacgcg ctgttcagga gtgagattgt ccggctgcgc gaggctccgg
     2281 aggccaggga gagagagttc tccgctccac tcggcggcct ccgcggcggt cggctcgtca
     2341 tccccgccgg catcttccag ttgagcctgc gcgcggacgc cgagatgacc gcgctgtatc
     2401 aggcccgctt tgagggggct gtgccagatg tgaagaccaa agatggcgag gtcaccattc
     2461 gctatccacg ccgtctgtgg gtgctgggcg ggaagatggg cgcggcggcg gtcacgctca
     2521 acgccgccat cccatggcag atcctcatcc agggcggcgc atcggacatc accgccgagc
     2581 tgggcaatct cgatctcgcc ggtttggagg tcaagggagg gatgagcatg atccgcctgc
     2641 atctccccgt gccctcccgc accgttccca tctacttcag cggcggagca tccgagatca
     2701 tcatgcgccg ccccgcgggc gtcgccgccc gcgtccacct caaaggctgg gcctcgacat
     2761 tcatcttcga cgatcaaaca ttcggcaatc taggtaacga cgtgcggctg caaagccctg
     2821 gctacgacac caccgcccag cgctacgaca tcgaagtcgc cagttccgcc agcatggtca
     2881 ccatcaccgc tgagtgacga tgcattcgtg ccacgctgcg ctgcaatcgc ctggctcagc
     2941 ggccgatggc gagccaggag agcagcgcgg cgcagggcat ccacgctcca acaacgagac
     3001 cggcgagtgc cgctcgcgag aggggaagat gta
//
LOCUS       DATDMP010000063         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1677327, whole genome shotgun sequence.
ACCESSION   DATDMP010000063 DATDMP010000000
VERSION     DATDMP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677327"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VI195_01090"
     CDS             <1..899
                     /locus_tag="VI195_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY6823006.1"
                     /translation="EWPRVGTETLMERSELRAGGSAGNAALAVSYLGGDSELLSVVGN
                     DEPGSWLAGQLRPLNASLATCDAPTTLTVGLIRNCGERNFFTTRGHLEALSYELLRPR
                     IAPAPAPESIALLSGVFLTPALRKCYPLLIRDLQALGYRVALDTNWPPEDWSAALRAE
                     VAGWISCCDHVLLNDLEVRSLADSDDLAVAIDRLAPVLRRGATLVVKTGARGALGIQD
                     GERCQHAAAQAAIFDTIGAGDSFNAGYLLARLDGAGLAASLAAGCEAATSIIARFPRR
                     SIRAGDLAGLRARVPTALEERA"
     gene            896..2128
                     /locus_tag="VI195_01095"
     CDS             896..2128
                     /locus_tag="VI195_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823007.1"
                     /translation="MRKSALLMLFIMVIWFVISFVTNLIGPLMPTIISDFHLSLFLAG
                     VLPFSFFIAYGLISIPAGALVEVRGPRTTLLTAFALNLAGCLAISLWPVYAVVIAGLF
                     LIGLGMAMLQVVINPLMRTTGGEAHFAFFAVMAQLVFGLASYVSPLIYAALMNQPGAA
                     ARPLVWVQFYWAFVVVYLILIAITATLRIPRLTLADNERSGSVATYRQLLGERTVQLF
                     FLGIIAYVGTEQSLADWMSQFLSSYHHFSPAREGAHAVGLFWGLMSLGCLLGMVLLRL
                     LDAKLVLAVFTILALICVGLALFGSAQISLLAFPTSGFFLSVMWSIIFSLALNSASRH
                     HGAFSGILCTGILGGAVVPLLVGVLGDHIGLRQAMLTVFVTLGFILSIAWWAQPLIRN
                     QTVGILELLSVRRGGAAH"
BASE COUNT          317 a          780 c          747 g          474 t
ORIGIN      
        1 ccgaatggcc gcgcgtcggc accgagactc tgatggagcg cagcgagctg cgcgcaggcg
       61 gctcggcggg caatgcggcg cttgcggtga gctatctcgg cggcgactcg gagctgctgt
      121 cggtggtcgg caacgatgag ccaggctcat ggctcgcggg acagctgcga ccactcaacg
      181 cctcgctcgc tacctgtgat gcgccgacga cactcacggt cggtctcatt cgcaactgcg
      241 gcgagcgcaa tttcttcacg acgcgcggtc acctcgaggc gctctcctac gagttgctgc
      301 gaccgcgcat cgcgcccgca cccgcgcccg agtcgattgc cctcctgtca ggagtgtttc
      361 tgactccggc gctgcgcaag tgttatccac tgttgattcg cgacttgcag gcactcggtt
      421 accgggtcgc actcgacacc aactggccgc ccgaggactg gagcgccgcg ctgcgtgccg
      481 aggtcgccgg ctggatctcc tgctgcgatc acgtgctgct gaatgatctc gaggtccgca
      541 gccttgccga cagcgacgac ctcgccgtgg ccatcgatcg gctcgcgccg gtgctcaggc
      601 gcggcgccac tctcgtcgtc aaaaccggcg cccgcggcgc tcttggcatc caggacggtg
      661 agcgttgcca gcatgccgcg gcgcaggcgg cgatcttcga caccatcggc gccggcgact
      721 ccttcaatgc cggttatctg ctcgcgcgcc tcgacggtgc cggcctcgcc gcctcgctcg
      781 ccgccggctg cgaggcggcc acctccatca ttgcgcgctt tccgcgccgt tcgatccgcg
      841 ccggtgatct cgccggcctg cgcgcccgag tccccaccgc gctcgaggag cgggcatgag
      901 gaagtcggcg ctgctgatgc tgttcatcat ggtgatctgg ttcgtcatct cgttcgtgac
      961 caacctcatc gggccgctga tgccgaccat catcagcgac tttcatctga gcctgttcct
     1021 cgccggcgtt ctgccgttct cgttcttcat cgcctacggc ttgatctcga ttccggccgg
     1081 agcactggtg gaggtgcgcg gaccacgtac tacgttgctg accgcgttcg cgctgaatct
     1141 cgccggctgt ctggcgatca gcctctggcc ggtgtacgcg gtggtgatcg cgggtctgtt
     1201 cctcatcggt ctcggcatgg caatgctgca ggtggtgatc aaccctctga tgcgcaccac
     1261 cggcggcgaa gcgcatttcg cgttctttgc ggtgatggcg cagctggtat tcggcctggc
     1321 ctcgtacgtg agcccgctca tctacgcagc actgatgaat cagccggggg cggcggcacg
     1381 cccgctcgtg tgggtacagt tctactgggc ctttgtggtc gtctacctga tcctgatcgc
     1441 gatcacggcg accctgcgca tcccgcgcct cacgctcgcc gacaacgagc gctcgggcag
     1501 cgtggctacc tatcgtcagc tgctcggcga gcgcaccgtg cagttattct ttctcggcat
     1561 catcgcttac gtcggcaccg agcagagcct cgccgactgg atgtcgcagt tcctcagcag
     1621 ctatcaccat ttctcgcccg cacgcgaggg ggctcatgcg gtgggcctgt tctggggcct
     1681 gatgtcgctc ggctgtctgc tcggcatggt gctgctgcgg ctgctggacg cgaagctggt
     1741 gctcgcggtt ttcaccatac tcgcgctgat ctgcgtcggc ctggcgttgt tcgggtcggc
     1801 gcagatttca ctgctggcat tccccacgag cggcttcttc ctctcggtta tgtggtcgat
     1861 catcttttcg ctggcattga actcggcgag ccgtcatcac ggcgccttct ccggcatcct
     1921 gtgcaccgga attctcggcg gcgcggtggt gccgctgctg gtcggtgtgt tgggcgatca
     1981 catcgggctg cgccaggcga tgctgacggt gttcgtcacc ctcggcttca tcctgagcat
     2041 cgcctggtgg gcgcagccgc tgatccgtaa tcagacggta ggaattctcg agttgttgag
     2101 cgtacgccgc ggcggcgcgg cgcattgaca ggcagggcgg gggaaatcac atgaagacac
     2161 gaccgacaaa cgtgcatcgc aagcacccgg tgtcagttgc cgtatccatg gcgttgctcg
     2221 ccgccgcggc gacctcggcc atggcaacag ggcccgcgga tgcccctgcg gagaatgcgg
     2281 gggcgcaggg ccctgcgccg cagaacccgc cgacgaca
//
LOCUS       DATDMP010000064         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_677442, whole genome shotgun sequence.
ACCESSION   DATDMP010000064 DATDMP010000000
VERSION     DATDMP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677442"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1700)
                     /locus_tag="VI195_01100"
     CDS             complement(129..1700)
                     /locus_tag="VI195_01100"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY6823008.1"
                     /translation="MTEPTRVCRRPGPSRDARRAMRAAPLPDNLKPVHPGMRSGRYRP
                     LDEADVLEIHRTALRLLAEVGLADAPPSGVEYLTRAGCTVSEHGRILFPSALVEDTVA
                     RAARHFVLHGQDPRHDMEPWGQRVYFGTAGAAVNMVDSHTGRYRESRIRDLYDCGRIV
                     DALEHIHFFQRTVVPRDIPDPYEMDFNTCYASVSATTKHVGTSWVQPLHVTRSLEMLH
                     EIAGGEDKWRARPFVSQSNCFVVPPMKFATDACRCLEVAALGGMPVLLLSAGQAGATA
                     PAALAGALAQEVAEVLAGLVYVNAVKPGAPAIFGTWCFVSDLRTGAMSGGSPEQALLS
                     AAAAQMAHYYDLTGGTASGMTDSKLPDAQSGAEKAYSHALVGNAGANLIYESAGMHAS
                     LLGFSLESLVIDNDIIGATQRTIRGIEVDEASLSFDTIREVCLQGPGHYLGSEQTLQL
                     MQSAYLYPVIGDRKSPNEWTEQGALDAAERAAKKVSELLATHFPAHVADAVDERIRAQ
                     FPVRLPREHMRTPAS"
     gene            complement(1850..>2056)
                     /locus_tag="VI195_01105"
     CDS             complement(1850..>2056)
                     /locus_tag="VI195_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HEY6823009.1"
                     /translation="GLSVKTGQTHVQRYSSKLLDMILNEELDTAFLISHRLPLEAAPE
                     GYRMFKEQQNEVTKVVLKPGAMLQ"
BASE COUNT          342 a          681 c          684 g          349 t
ORIGIN      
        1 cagcaacaca tcgccacagc cgcgcttggc aagatgatag gcgacgctgc agccgacgat
       61 gccgccgccg atgatcacga cgttcgcgtg cgacggaatg cctggcacgg cgatctcggg
      121 ctccgggctc agctcgcggg agtgcgcata tgctcgcgcg gcaacctcac ggggaactgc
      181 gcgcggatcc gctcatcgac cgcatccgct acgtgggccg gaaagtgcgt cgccagcagc
      241 tcgctgactt ttttcgcggc ccgctcggcg gcatcgagcg ccccctgctc cgtccattca
      301 ttcggactct tgcgatcgcc gatcactgga tagaggtagg ccgactgcat cagctgcagg
      361 gtctgctcgg agccgaggta gtggccgggc ccctgcagac acacctcacg gatggtgtcg
      421 aaggacaggc tggcctcatc gacctcgatt ccgcggatcg tccgttgtgt cgcgccgatg
      481 atgtcgttgt cgatgacgag gctctcgagc gagaagccga gcaggctcgc atgcatgccg
      541 gcggactcgt aaatcaggtt ggcgccggcg ttgcctacca gcgcgtggct gtaggctttc
      601 tcggcgcccg actgcgcatc gggcagcttc gagtcggtca ttcccgaggc cgtgccaccg
      661 gtcaggtcgt agtaatgcgc catctgcgcg gcggccgccg agagcagcgc ctgctcgggg
      721 ctgccgccgg acatggcccc ggtacgcagg tccgatacga aacaccaggt tccgaaaatc
      781 gccggtgctc cgggcttgac ggcattcacg tacacgagcc ccgccagcac ttccgcgacc
      841 tcctgcgcca gagcccccgc cagcgccgcc ggcgccgtgg cgccagcctg cccggccgac
      901 agcaacagca ccggcatgcc accgagtgcg gccacctcga gacagcggca ggcatcggtc
      961 gcgaacttca tgggtggaac cacgaagcag ttcgattggc tgacgaaagg gcgcgcacgc
     1021 cacttgtcct caccgcccgc gatctcatgc agcatctcga gcgagcgcgt cacatgcagc
     1081 ggctgaaccc aactcgtgcc tacgtgcttg gtcgtcgccg acaccgaggc gtagcaggtg
     1141 ttgaagtcca tctcgtaggg gtcgggaata tcgcgcggca ccacggtgcg ctgaaagaag
     1201 tggatgtgct cgagcgcatc gacgattcgt ccgcagtcgt acaggtcgcg aattctggat
     1261 tcacgatagc ggccggtgtg cgaatcgacc atgttgacgg cagctccggc ggtgccgaag
     1321 tacacccttt gtccccacgg ctccatgtcg tggcggggat cctggccgtg cagcacgaaa
     1381 tgacgcgcgg cgcgcgccac agtgtcctcg accagcgcgc tcggaaaaag aatccggcca
     1441 tgctcgctca ccgtgcagcc cgcgcgtgtc aggtactcga cccccgaggg tggtgcgtcg
     1501 gcgagtccca cttccgccag caaccgcagc gccgtgcggt gaatctcgag cacatcggcc
     1561 tcgtcgagcg gccggtagcg accgctccgc atgccggggt gcaccggctt gagattgtca
     1621 ggcagcggcg ccgcgcgcat ggcgcggcgg gcgtcgcggc tcggtccggg gcgtcggcag
     1681 acacgggtcg gttccgtcat ggcggtcagt cctaacggcc cggcggaggc cgccctcggc
     1741 cctcctgctg gggcttgcgt gaagatgagt attgcagcgc ccgagcaggg gcaccagagg
     1801 ggtcgcggag ccgatactcg gccgaggcac cgggagccgg tatcgtcggt tactgcagca
     1861 ttgcacccgg cttgaggacg accttggtca cctcgttctg ctgctccttg aacatccggt
     1921 aaccctcggg cgctgcttcg agcggcagcc gatggctgat gagaaacgcg gtgtccaact
     1981 cctcattgag aatcatgtcg aggagcttcg aggagtatcg ttgcacgtgg gtctgtccgg
     2041 tcttgactga caggcc
//
LOCUS       DATDMP010000065         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_900157, whole genome shotgun sequence.
ACCESSION   DATDMP010000065 DATDMP010000000
VERSION     DATDMP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900157"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VI195_01110"
     CDS             complement(<1..411)
                     /locus_tag="VI195_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823010.1"
                     /translation="MLGLPTRLLRQHAGLLGFMLGAAGTLTTEVGKNCGDERHGGKYR
                     AGGRDLSGARCRTSRREFAFLLLREQPLGGLACLTLLTQVAACLDDPTENIVSQLDTA
                     DIESLFDAQETSVHEHGERAGLSARLGESLDEALL"
     gene            376..>2084
                     /locus_tag="VI195_01115"
     CDS             376..>2084
                     /locus_tag="VI195_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823011.1"
                     /translation="MLAEQARGQAEQLLAYLSDDFARELENFGRLEVLAEFSQRQIDY
                     FHGLPQALRGADTVRNGALALVNHSRALRALGQLEVAGSNADEAVQLLRTLHDSAGRS
                     EATTVGLARAYAAKARVLESRGDPTGMLTSEQAIAVLQPLLAAPKPPLAARRAYVEIA
                     TRLGYQQGAASHREDAARTLRSAMQAAADLGGRDIRDPEMGAWYAEAGGWLVTVLESL
                     GQYPEAGRVSRECLAVADQVLERRPGYRLVLHAQQILVGVLVSVSKDELDPQAARQAA
                     VRQEQVSLSLLNLDPNNVTSLNNMAVAVDQLADTLWQAGQLRESVAHRLREVDYARRA
                     AAGGASFVSNYTGTLAGVSYFQAATGDHAGADAMLASAEPEVIKLRKTDPGGDGAVIA
                     EGWLRVGAAYAALLRDDLAATRRIAAEAVRELEGITPHGELQFRKTFQLYFLHDADGR
                     AAYLSGDAAAAEQSLLKAVEARTTAGTGGMDDRRQLGMLHTWLALAQARQGHTGQAAQ
                     TIAPAVKFQRELAARNRGDRWQPQELAAAVYAQALTDPADRTSLLNEAARLMDRLASE
                     VRAT"
BASE COUNT          339 a          692 c          726 g          327 t
ORIGIN      
        1 gaggagagcc tcgtcaagcg attcgccgag gcgggcgctg agtccagcac gctcgccgtg
       61 ctcgtgaacc gacgtctctt gcgcatcgaa gagcgactcg atgtccgccg tgtcgagctg
      121 actcacgatg ttctctgtgg ggtcgtcaag gcaagccgcg acctgcgtca ggagcgtgag
      181 gcacgcgagg ccaccgagcg gttgctcgcg cagcagaagg aacgcgaact cgcggcgcga
      241 cgtgcggcaa cgcgcgcccg acagatcgcg gccgcctgca cggtacttgc cgccgtggcg
      301 ctcatcgccg cagtttttgc ctacttcagt agtcaacgtg cccgccgcgc cgagcatgaa
      361 gcccagcaga cccgcgtgct ggcggagcag gcgcgtgggc aggccgagca actgctcgcg
      421 tacctcagcg acgactttgc gcgtgagctc gagaacttcg gacggctcga ggtgctggcg
      481 gagttctcgc agcgccagat cgattacttt cacgggctac cgcaggcgtt gcggggagcc
      541 gacacggtgc gcaacggtgc actcgcgctc gtgaatcatt cgcgggcgct ccgcgcgctc
      601 ggccagctgg aggtcgccgg cagcaatgcg gatgaagccg tgcagctcct ccgtacactt
      661 cacgattcgg caggtcgctc cgaggcgacg acggtggggc tcgcacgcgc ctacgccgcg
      721 aaggcacgtg tcctggagag ccgtggcgac cccaccggca tgctgaccag tgaacaggcg
      781 atcgcggtat tgcagccgct gctcgccgcg cccaagccac ccctggcggc caggcgggcc
      841 tacgtcgaga tcgctacccg tcttggctac cagcagggcg ccgcatctca tcgggaggac
      901 gcggcgcgca ccctgcgctc ggccatgcag gcggcggccg atctcggagg ccgggatatc
      961 cgcgatccgg agatgggcgc ctggtacgct gaagccgggg gttggctggt gaccgtcctg
     1021 gagagtctgg ggcagtaccc cgaagcggga cgcgtgagcc gtgagtgtct ggcggtggcc
     1081 gaccaggtgc tggagcggcg ccctggctac cggcttgtgc tgcatgcgca gcagatactg
     1141 gtcggagtgc tcgtgtccgt gagcaaggac gagctcgatc cgcaggcagc acggcaggca
     1201 gcggtgcgtc aggagcaggt gtcgctcagc ctgctgaatc tcgatcccaa caatgtcacg
     1261 agcctgaata acatggccgt cgcggtggac cagctcgccg acacgctctg gcaggccgga
     1321 cagctccgtg agtcagtggc gcatcgcctg cgtgaggtcg actacgcacg tcgcgccgcc
     1381 gcgggcggtg cttcctttgt ctccaactac accggcacgc tggccggggt ctcgtatttc
     1441 caggccgcca cgggcgatca cgccggagcg gatgcaatgc tcgcgtccgc cgagccggag
     1501 gtcatcaagc tccggaaaac tgatccgggc ggagatggtg ctgtaatcgc cgagggctgg
     1561 ttgagggtcg gcgcggccta cgctgccctg ctgcgcgatg atcttgccgc gacccggcgc
     1621 atcgccgccg aggcggtgcg ggaattggag ggaatcacgc cgcacggtga actgcagttc
     1681 cgcaagacgt tccagctcta cttcctccac gatgccgacg gacgcgcggc atacctctcc
     1741 ggggatgccg ccgccgcgga acagtccctg ctcaaggccg tggaagcccg cacgacggct
     1801 ggcaccggcg gcatggatga tcggcggcag ctcgggatgc tgcacacgtg gctcgcgctg
     1861 gctcaggccc gtcaaggcca caccggtcaa gccgcccaga ccatcgcacc ggcggtgaag
     1921 tttcagcgcg agctcgccgc gcgcaaccgt ggcgatcggt ggcaaccgca ggaacttgcc
     1981 gccgcggtct atgctcaggc tctgactgat cctgcggacc gcacctcact gttaaatgag
     2041 gccgcgcgcc tcatggatcg cctcgcgtcc gaggtgcgcg caac
//
LOCUS       DATDMP010000066         2660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_11561, whole genome shotgun sequence.
ACCESSION   DATDMP010000066 DATDMP010000000
VERSION     DATDMP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11561"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1908
                     /locus_tag="VI195_01120"
     CDS             337..1908
                     /locus_tag="VI195_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823012.1"
                     /translation="MEEPQESARSRSEPAAAAHPAAAAPPEAAKPRDPEQTGLNFIDP
                     EMFLAQGQASTAVPVVNATEQSAKAPEPTTELTLEDPVVDAMLTSPPPPPAAAPPAAP
                     APSAAAAPKPESPADPAAARTAARVPIAKTASLTAAATKLAAASAAPRAAIKTAAARK
                     PDAAAVPIAKPAVQSEGAPKPPPPPPATPTSTATAPKPENPAPKLDAPSLASWANARA
                     AASPKVRPATTTAAAATAATNITGAPKGVPPRTSSAPPASAPASVAPASAAAQTPATS
                     SPAVATTPAVPPAPVMDRDFIARNQVVERYLSGRLPLKAATEFEHFCRDHSELLDEIG
                     LPERVNAGLRLLEASGKPEPWQEAPRPVWQKPQVTLGLAVTVLVLVLALAGVAANSSS
                     RSHRIAELQKQNSERALDPATSTREIRLLPSRSGASNTPAIIIGGGGVTQLVDFKIDE
                     SRSAYRTFRVTIDRIDQGRVAIISNLDKDSNGHLRIALNSSALGPGNYQLTLEGLDWR
                     GQPQPDSWVTIGIQR"
     gene            complement(1915..>2660)
                     /locus_tag="VI195_01125"
     CDS             complement(1915..>2660)
                     /locus_tag="VI195_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6823013.1"
                     /translation="IAGRWWSAADAGQPLVSLASEFQEAIGVQVGDRLTFDIAGETRE
                     VTVASIRKVKWDTFQPNFFIVFPPGVLEGTAGTYLTSAYFAPGAARSLAQLAHRFPSV
                     SIFDIDELLAQVRAVLDKAALAVQSVFVFTLFAGLTVLLAAVQSSRDERRYESAMLRT
                     LGASRGTVVQGVLAEFATLGSLSGLLAALGASLAAYYLTTQWLELHYVFELLPWIEGL
                     VGGALLVAAGGWLATRSVIDQPPLSTLRA"
BASE COUNT          479 a          968 c          842 g          371 t
ORIGIN      
        1 atcgtcaatc tgcgcctggt gcgcagcctg cgcgcacaca tgaatggcga gtacttcctg
       61 acactcgagg gcggtcacga gctcaagctc tcgcgcacct accgcgacaa ggtcgagtat
      121 ttcctcaacg ggtccgcccg ctaggaatgt ggcggcgtga tgcagttcgc gccgcagctg
      181 cgctggcggt gcaagtgtga actacgtcac gaacacggca cgcatggcgg cgatgtgtga
      241 gccatcgcga cgcggtgcag cggctgcgtt gcttagatat gtctacctat ggtagccgcg
      301 tcccgcccgc cgctcgaggc cctcccggat ttgccggtgg aagagccgca agagtctgcc
      361 aggtcgcgca gcgagcccgc cgcggccgcg caccccgctg ccgctgcgcc gcctgaagcg
      421 gcaaagccgc gggatcccga gcagactgga ctgaatttca tcgacccgga gatgttcctg
      481 gcgcagggac aggcgtcgac cgccgtgcca gtggtcaatg cgaccgaaca aagcgcgaaa
      541 gcgcccgagc ccaccactga gttgacgctc gaagaccccg tggtcgatgc gatgctcact
      601 tcgccgccgc cgccgcctgc agcggcgcca ccagccgcac ccgcgccgtc tgccgcggcc
      661 gcgccaaaac ccgagtcgcc tgcggacccc gcagccgccc gcactgccgc acgtgtgccg
      721 atcgcgaaaa ccgcatcgct gacggcggcg gctaccaagc tcgcggcggc gagcgcagct
      781 ccgcgcgccg ctatcaagac cgccgcagcg cgcaagccgg acgccgccgc cgtgcccatc
      841 gcaaaacccg ccgtgcaaag cgagggcgcg ccgaagccgc cgccgccgcc accggccacg
      901 ccgacttcga cagctacggc gccgaagccg gagaatccgg ctccgaagct cgacgcgccg
      961 agtctcgcca gctgggcaaa tgcgcgtgca gccgcctccc cgaaggtccg ccctgcgacg
     1021 acgactgcgg ctgccgctac ggcagcgacg aatatcaccg gcgctcccaa aggcgtcccg
     1081 cctcgcacct catccgcccc cccggcgagt gcgccagcca gcgtcgcgcc tgcatccgcg
     1141 gcagcacaaa ctccggcgac ttcatccccc gccgttgcca cgacacccgc cgtaccgccg
     1201 gcgccggtga tggatcgcga tttcattgcg cgcaaccagg tggtcgagcg ttacctctcg
     1261 gggcgattgc cgctcaaggc cgcgacggag ttcgagcact tctgtcgcga tcactccgag
     1321 ctgctcgatg agatcggcct gccggagcga gtcaacgcgg gtttgagact gctcgaggcg
     1381 agcggcaagc ccgaaccctg gcaggaagcg ccacggccgg tatggcagaa gccccaggtg
     1441 acgctaggcc tggcggttac cgtcttggtg ctggtgctgg cgctcgccgg tgtcgccgcc
     1501 aacagcagca gcaggagtca ccgcatcgcc gagctacaaa agcagaattc cgaacgtgcg
     1561 ctcgatcccg ccaccagcac ccgtgagatc cgcctgctgc cgagccgctc cggcgcatcc
     1621 aatacaccgg ccatcatcat cggcgggggc ggggtaacgc agctcgtaga tttcaagatc
     1681 gatgaatcgc gctcggccta ccgcaccttc cgggttacca tcgaccgcat cgatcagggg
     1741 cgcgtcgcga tcatcagcaa tctcgacaag gactccaacg gtcacctgcg cattgcactc
     1801 aacagttccg ccctcggccc gggcaactat cagttgacgc tcgagggcct cgattggcgc
     1861 ggccagccgc agccggactc ctgggtcacg atcggcatcc aacgctgagg cggctcaggc
     1921 gcgcagcgtg ctcagcgggg gctgatcgat gacgctgcgc gtggcgagcc aaccccccgc
     1981 cgcgaccagc agtgccccgc ccacgaggcc ctcgatccac ggcagcaact cgaacacgta
     2041 gtgcagctcg agccactggg tcgtgaggta gtacgccgcc aatgacgcac cgagtgccgc
     2101 cagcagtccc gaaaggctgc cgagggtggc aaactcggcc agcactccct gaaccacggt
     2161 gccgcggctc gcaccgagcg tacgcaacat cgcgctttcg taacggcgct catcgcggct
     2221 agactgcacg gccgccagca gcacggtgag cccggcgaac agcgtgaaga cgaacacgct
     2281 ctgcaccgcg agcgccgcct tgtcgagcac ggcgcgcact tgcgcgagca actcatcgat
     2341 gtcgaagatc gagacactgg ggaagcgatg tgcgagctgc gccagcgagc gtgccgcgcc
     2401 cggcgcgaaa taggcgctgg tcaggtaggt acccgcggtg ccctccagca ctcccggcgg
     2461 gaacacgatg aagaagttcg gttggaaggt gtcccacttc accttgcgga tgctcgcgac
     2521 cgtgacctcg cgcgtctccc cggcgatgtc gaaggtcagc cgatcaccga cctgcacacc
     2581 gattgcttcc tggaactcgc tggcgagcga taccagcggc tggccggcgt ccgcggcgct
     2641 ccaccagcga ccggcgatga
//
LOCUS       DATDMP010000067         5919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1566954, whole genome shotgun sequence.
ACCESSION   DATDMP010000067 DATDMP010000000
VERSION     DATDMP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566954"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VI195_01130"
     CDS             <1..570
                     /locus_tag="VI195_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY6823014.1"
                     /translation="ESFGLDRPRGALVSSVEKDGPAARAGVAPGDVILAVDGRPIERY
                     GELSSQIAAMRPGGDASLALWRNGKEQTLNVKVAELKEQPQSVAYRSGKPKQPAAAQP
                     SALGLTVRPLEPREKEQANTQGSLLVEEVSGPAQAAEIQPGDIILGINGKRVHSVKEL
                     EEAARAAGKNVALLIQREDAQIFVPLRLP"
     gene            577..3195
                     /gene="leuS"
                     /locus_tag="VI195_01135"
     CDS             577..3195
                     /gene="leuS"
                     /locus_tag="VI195_01135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY6823015.1"
                     /translation="MHESYDPAAVERAAQQYWEERRAFEVREDPARAKFYCLSMLPYP
                     SGRLHMGHVRNYTIGDVLARFLRMQGHNVLQPMGWDAFGLPAENAATANGVPPAKWTR
                     ENIAHMRAQLKSLGFAIDWSREIATCDPGYYRWNQWLFLRMLEKGLAYRKTGVVNWDP
                     VDQTVLANEQVIEGRGWRTGALVEKREIPMYYLNITRYADELLESLDALPGWPERVRV
                     MQANWIGRSAGCEIAFPYAADAAAVAGAAGVVKVFTTRADTLFGATFLAVAAEHPLAL
                     AAARSDARLAAFIEECRRGSVIEAEVATQVKKGMPTGLHVRHPFTGAPLPIWVANYVL
                     MAYGEGAVMGVPAHDERDFEFALGHGLAVTTVVRSLSDAYVEVRAPWVAAYSEYGVTV
                     NSGEFSGLASRAAMDAIATALEHKGLGRKRVQYRLRDWGISRQRYWGCPIPIIHCAAC
                     GVVPVPEEALPVRLPEDLVPDGSGNPLAKSAAFYECTCPRCRGPARRETDTMDTFVDS
                     SWYFLRYACHDAANMVDARVDYWAPVDQYIGGIEHAILHLLYARFWTKVMRDFGLLSF
                     GEPFSNLLTQGMVLNHVYSYQAEGNRKRYFNPAEVSTHRAADGSEINEVVTPDLGRLR
                     VTHEGLGKMSKSEGNGVDPEGLVERFGADTARLFIMFASPPEQTLEWSDEGVQGAWRF
                     IRRLWTAVYEHLAAGAAPPLEPHALTPGERDLRRAAHQALAKATDDIGRRRNFNTAIA
                     AVMELLNAVQRFQAMSSQARAVRQEALGLAVLMLSPITPHVCHALWLALGHTTAPIDE
                     RWPVPDSEALAQETHAIVVQVNGKLRGHISVPVSADEAAVRAAALADSNVRKFVADRP
                     VRRVIIVPGKLVNVVV"
     gene            3218..3775
                     /gene="lptE"
                     /locus_tag="VI195_01140"
     CDS             3218..3775
                     /gene="lptE"
                     /locus_tag="VI195_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY6823016.1"
                     /translation="MASHGISAVNKTRDFPGTPVKSRSGARVRIWSGLLAAALLAGCG
                     FHLQGRTPLPPVVRTPYVEAPDRQSDFVQYLRHALLSNGAQLTPQKEQASAVVSILRD
                     SVARRVLSVSATNQPNQYEVTYTVGFSVSAAGKELLPPQELTATRTYSFDERLLLAKN
                     HEEEILRQDMAHDLADMVMRRLASL"
     gene            3810..4334
                     /locus_tag="VI195_01145"
     CDS             3810..4334
                     /locus_tag="VI195_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823017.1"
                     /translation="MPAPLTVPSPTPAVLLLSGIDRVAVALLLARYGLTLTLVAAGEA
                     IPGSYWGDSEAGLQEDALFARLDTPLHSVLHEACHYVCMTPERRAGLDRDAGGDDIEE
                     AAVCYLQVLFADELPHVGRERLFTDMDAWGYSFRLGSTRAWFEGDAQDARAWLSQYGI
                     IDPAGLLTGAPRSA"
     gene            4434..4757
                     /locus_tag="VI195_01150"
     CDS             4434..4757
                     /locus_tag="VI195_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823018.1"
                     /translation="MTMRRSSLALLLSFLLVFVQQGAVLHELGHLSGGANHAGAALRA
                     DVQPASGAICQRCEAYAQVANPAGGATASVPLDLAGLLRIPDPRYAIATADSPTPRSR
                     GPPSV"
     gene            4839..>5919
                     /locus_tag="VI195_01155"
     CDS             4839..>5919
                     /locus_tag="VI195_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823019.1"
                     /translation="MVNSRCGRASAAVAASVMWLAAAMFAAPVLEAAEASDATAADAE
                     GQLAPVVVSAKRLNAARSEIETQTGASTYTIDSQGIAGAPGGENQSLSQILLQAPDVV
                     QDSFGQLHVRADHNDLQYRLNGIILPEGISVFSQTLSPRLISSLRLITGALPAEYGLR
                     TAGIIDLSTNSGTLQPGGTASVYGGSFGTFQPAAEYGGRSGNFSYYMTGDYKQSDLGI
                     ESPDGSRDPLHDHTQQVHAFGYFENLLGANDRLSLIAGLSNGEYQIPNQRGLQPALGL
                     DVNGQTEFLSDALDQNQHELAEYAILAWQHADDALNWQSALSARYTSLHFMPDPVGTL
                     LYTGIAQDAFKDDTAFAWQTDAAYTL"
BASE COUNT          971 a         1991 c         2001 g          956 t
ORIGIN      
        1 gagtccttcg gactcgatcg accgcgcggt gcgctggtgt cctccgtgga gaaggatgga
       61 cccgccgcgc gcgccggcgt cgcgccgggc gatgtaattc tcgcggtcga cggccgtccg
      121 atcgagcgtt atggcgagct ctccagccag atcgccgcga tgcgtcccgg cggggacgcg
      181 agtctggcgt tgtggcgcaa cggcaaggag cagacgctca acgtcaaggt cgcggagctc
      241 aaggagcagc cgcagagcgt cgcgtaccgc agcggcaaac cgaagcagcc cgcagccgct
      301 caacccagtg cactcggtct caccgtacgt ccgctcgagc cgcgcgagaa ggagcaggcg
      361 aatactcagg gaagcctgct ggtggaggag gtctcggggc ccgcgcaggc ggcggagatc
      421 cagccgggcg atatcatcct cggcatcaac ggcaagcgcg tgcactcggt aaaggagctc
      481 gaggaggcag cgcgcgccgc gggcaagaac gtagcgctgc tgatacaacg tgaggacgcg
      541 cagatcttcg taccgttgcg tctcccataa caggtaatgc acgagagcta tgaccccgcg
      601 gcggtcgagc gcgccgcgca gcagtattgg gaagagcgac gtgccttcga ggtgcgcgag
      661 gatcccgcgc gcgccaaatt ctattgcctg tcgatgctgc cttatccgtc cggacggctg
      721 cacatgggcc acgtgcgcaa ctacaccatc ggcgatgtgc tggcgcgctt cctgcgcatg
      781 cagggtcaca acgtactgca accgatgggt tgggacgcct tcggcttgcc ggccgagaac
      841 gccgctaccg ccaacggcgt gccgccggcg aagtggacgc gggaaaacat tgcccacatg
      901 cgcgcgcagc tcaagtcgct cggcttcgcc atcgactgga gccgcgagat cgcgacctgc
      961 gaccccggct actaccgctg gaaccagtgg ctgttcctgc gcatgctgga aaagggactc
     1021 gcctatcgca agaccggagt ggtgaactgg gacccggtcg atcagacggt gctcgccaat
     1081 gagcaggtga tcgaggggcg cggctggcgc accggcgcgc tggtggagaa gcgcgagatc
     1141 ccgatgtact acctcaacat cacgcgttac gcggatgagc tgctcgagtc gctcgatgcg
     1201 ctccccggct ggccagagcg cgtgcgggtg atgcaggcga actggatcgg acgcagcgca
     1261 ggctgtgaga tcgcctttcc ttatgccgcc gacgccgccg ccgtggcggg tgccgccggt
     1321 gtcgtgaagg tattcaccac tcgcgccgac accctgttcg gcgccacctt tctcgccgtc
     1381 gccgcggagc atccgttggc gctggccgcc gcacgcagcg acgcgcgcct tgccgctttc
     1441 atcgaggaat gccggcgcgg cagcgtcatc gaggcggagg tggccacgca ggtgaagaag
     1501 ggcatgccga cggggctgca cgtgcggcat cccttcaccg gcgcaccgct accgatctgg
     1561 gtcgccaact acgtgctcat ggcgtacggc gagggcgcgg tgatgggcgt gcccgcccac
     1621 gacgagcggg acttcgagtt cgcactcggc cacgggctgg cggtgaccac ggtggtacgc
     1681 tcactcagcg atgcctatgt cgaggtgcgc gcaccctggg tggctgcata tagtgagtac
     1741 ggcgtgacgg tgaactccgg ggagttttcc gggcttgcct cgcgggcggc gatggatgcg
     1801 atcgccacgg cgctcgagca caaggggctc ggcaggaaac gcgtgcagta ccgcttgcgc
     1861 gattggggca tctcccgcca gcgctactgg ggttgcccca ttccgatcat tcattgcgcc
     1921 gcctgcggcg tcgtaccggt gccggaggag gcgctgccgg tacgcctgcc cgaggatctc
     1981 gttcccgatg gcagcggcaa tccgctcgcc aagtcggccg cgttctacga atgtacatgt
     2041 ccgcgctgcc gcggaccggc ccggcgcgaa accgacacca tggacacctt cgtggactcg
     2101 tcctggtact tcctgcgcta cgcctgtcac gatgccgcca acatggtgga cgcgcgggtg
     2161 gactactggg cgccggtcga tcaatacatc ggcggcatcg aacacgccat cctgcacctg
     2221 ctgtatgcgc gcttctggac caaggtcatg cgggatttcg ggctgctgag cttcggcgag
     2281 cccttcagca atctgctcac ccagggcatg gtgctgaacc atgtctactc gtaccaggcc
     2341 gagggcaacc gcaagcgcta cttcaacccg gccgaggtca gcacccaccg cgctgcggac
     2401 ggcagcgaga taaacgaggt ggtgacgccc gacctcggca ggctacgggt gacgcacgaa
     2461 ggtctcggca agatgtcgaa gtccgagggc aacggtgtcg atccggaagg actcgtcgag
     2521 cgcttcggtg ccgacacggc gcggctcttc atcatgttcg cctcgccgcc cgagcagacc
     2581 ctcgagtggt cggacgaggg ggtgcagggt gcgtggcgtt tcatccgccg cttgtggacc
     2641 gcggtgtacg agcacctcgc cgccggcgcg gccccgccac tcgagccaca cgcgctcact
     2701 cccggtgagc gcgatctgcg gcgcgcggcg catcaggcgc tcgccaaggc aaccgacgac
     2761 attggccggc ggcgcaattt caacaccgcc atcgccgcgg tcatggagct gctgaacgcg
     2821 gtgcaacgct tccaggcgat gagttcgcag gcacgcgccg tgcgccagga ggcactggga
     2881 ctcgccgtac tcatgctctc gccgatcacc ccgcacgtct gccacgcgct gtggctcgcg
     2941 ctcggacaca cgacggcgcc gatcgatgag cgctggccgg tacccgacag tgaggcgctt
     3001 gcccaggaga cgcacgccat cgtcgtgcag gtgaacggca agctgcgcgg ccacatcagt
     3061 gtgccggtga gcgccgatga ggctgcggtg cgcgcggcag cgcttgccga cagcaacgtc
     3121 aggaagttcg ttgccgaccg gccggtgcgg cgcgtcatca tcgtgccggg caagctcgtc
     3181 aacgtagtcg tgtgaagcac cccaggaaat cgcgcttgtg gcatcccatg ggatctctgc
     3241 agtcaacaag acgcgcgatt tcccggggac cccggtgaag agccggagtg gggcgcgtgt
     3301 taggatctgg agcggcctgc tcgcggcagc gttgctcgcc ggttgcggat tccacctgca
     3361 gggacgcacg ccgctgccgc cggtggtgcg cactccatac gtcgaggctc ccgaccgcca
     3421 gagcgatttc gtgcagtatc tgcggcacgc gctgctgtcg aacggcgcac agctgacgcc
     3481 gcagaaggag caggcatcgg ccgtggtgag cattctgcgc gacagcgtgg cgcggcgcgt
     3541 gctgtcggtg tcggccacca accagccgaa ccagtacgag gtgacctaca cggttggatt
     3601 ctcggtgagc gccgccggca aggagctgct gcctccacag gagctgacgg ccacgcgcac
     3661 ctacagcttc gatgagcggc tgctgctcgc caagaatcac gaggaggaaa tcctgcgcca
     3721 ggacatggcg cacgatctcg ccgacatggt catgcgacgc ctggcgagcc tctgacatcc
     3781 ggcgctgcgc cgtttgccct tcgcccgtta tgccagcgcc cctgaccgtc ccgtccccga
     3841 cgcccgcggt gctgctgctg agcggcatcg atcgcgtggc ggtcgcgttg ctgctcgccc
     3901 gctatgggct gacgctcacg ctggtggctg ccggggaggc gattcccgga tcgtactggg
     3961 gggacagcga ggcaggtctc caggaggacg ccctctttgc ccggctcgat actccgctgc
     4021 actcggtgtt gcatgaggcg tgtcattacg tctgcatgac cccggaacgc cgtgccgggc
     4081 tcgatcgcga cgccggcggg gatgacatcg aggaggcggc ggtgtgttac ctgcaggtgc
     4141 tgtttgccga cgagctaccg cacgtcgggc gcgagcgcct gttcaccgac atggacgcct
     4201 ggggctacag cttccgtctc ggcagcaccc gcgcatggtt cgaaggggac gcgcaggacg
     4261 cgcgtgcctg gctgagccag tacggcatca tcgacccggc cggtctgctg acgggcgcgc
     4321 cgcgctcggc ctaggccggc cgctcagcgc aggctgccgc tttcgtgcgc tgcgccctgg
     4381 caagcggcgg cgctggcacg ttagactccg cgccgccctg gcggcccgtt gtcgtgacta
     4441 tgcgccgatc ctcgctggca ctgctgctgt cgttcctgct ggtgtttgtc cagcagggcg
     4501 cggtgttgca cgagctcggg cacctgagcg gcggggccaa ccatgccggg gcggcgctac
     4561 gtgccgacgt gcagcccgcg agcggcgcta tctgccagcg ctgcgaggca tacgcacagg
     4621 ttgccaaccc ggccggtggc gcgacggcga gcgtgccgct ggacctcgct ggcctcctcc
     4681 gcatacccga tccgcgctac gcgatcgcca cggccgactc gcccactccc cgcagccgcg
     4741 gacctccttc cgtctgaact gagcttcgat tcccggacgc tgcagccccg agtcgcgtgg
     4801 gcgcgcggcg tgacatttca gttcgtgcgg agtgtttcat ggtcaattcc cgatgtgggc
     4861 gcgccagcgc agcggtggcc gcctcagtga tgtggctggc agccgccatg ttcgcggcgc
     4921 ccgtgctcga ggcagcggaa gcgagcgacg caaccgcagc cgacgcggag ggccagctcg
     4981 cgccagtcgt ggtttcggcg aaacggctga atgctgcgcg ctcggagata gagacgcaga
     5041 ctggagcctc gacctacacc atcgacagcc aggggattgc cggagctccc ggcggtgaga
     5101 accagtcgct gagtcagata ctgctgcagg caccggatgt cgtgcaggac tccttcggac
     5161 aactgcacgt gcgcgccgac cacaacgatc tgcagtatcg gctgaacggc atcatcctgc
     5221 ccgagggcat cagtgtcttc agccagacgc tcagtccccg gctgatctcc tcgctgcgac
     5281 tcatcaccgg cgcgctgccg gcggagtacg gcctgcgcac tgccggcatc atcgacctca
     5341 gcaccaacag cgggacgctg cagcccggcg gaacggcgtc ggtatacggc ggcagcttcg
     5401 ggactttcca gccggcggcc gaatacggcg gccgctcggg caatttcagc tactacatga
     5461 ccggcgacta caagcagagc gatctgggca tcgaatcgcc cgatggcagc cgcgatccgc
     5521 tgcacgatca cacccagcag gtgcacgcct tcggctactt cgagaacctc ctcggtgcga
     5581 atgaccgctt gtcgctgatc gccggccttt ccaacggcga gtaccagatt cccaatcagc
     5641 gcggcctgca gccggcgctg ggcctggatg tcaacggcca gaccgagttc ctgagcgacg
     5701 ctctcgacca gaatcagcac gagctggccg aatacgccat cctcgcctgg cagcacgcag
     5761 acgacgcgct caactggcag tcagcgctct cggcgcgcta caccagtctg cacttcatgc
     5821 ccgacccggt tggcaccctg ctgtacaccg gcatcgccca ggacgcgttc aaggatgaca
     5881 cggccttcgc ctggcagacc gacgccgcct acacgctgg
//
LOCUS       DATDMP010000068         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1011498, whole genome shotgun sequence.
ACCESSION   DATDMP010000068 DATDMP010000000
VERSION     DATDMP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011498"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VI195_01160"
     CDS             complement(<1..350)
                     /locus_tag="VI195_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823020.1"
                     /translation="MKRNNLIAITVAAIMGAALSTAYADEQWTPVPDNQPANQQAPNT
                     GAAQTGEQQWSDRDGQRSGDHRYGDERRDGHDVSSNRRDIARDRADIRSDRQDIQRDR
                     ADVRADERDLHHDYA"
     gene            complement(393..1007)
                     /locus_tag="VI195_01165"
     CDS             complement(393..1007)
                     /locus_tag="VI195_01165"
                     /inference="COORDINATES: protein motif:HMM:NF021515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HEY6823021.1"
                     /translation="MHGSANTTNRYRAVLLVAVALTCAGCASRKVDVAQQSISTQAPF
                     SRTFEGAGDAVCWSVKRALLSQGYMLDRPGESGVLTGTRDFQPDPKLNVSYHLQTTCA
                     DNRDGSSIVFVTAEREDSRLQKMKQSTSVGVGPATLTMPSGSAQVLGVVRRETLKDPA
                     FYARFFALVQSYIDQEQERAAHERKPHEERHAEVRSDAQAPENR"
     gene            1302..>2048
                     /locus_tag="VI195_01170"
     CDS             1302..>2048
                     /locus_tag="VI195_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823022.1"
                     /translation="MHRIAAALMLMAAPGAFAYTAAELAAKNVAAKGGAEKLDAIQSL
                     RMSGKLLLRGGAVQVGYTMLVERPHAIRYEVQLQGLTRVQAYDGSEAWQINPFQGRKD
                     PEKLSADDAKALAEDAADFTGSLVDYQAKGYRLDYLGTEDIDGTEAHKLRVTRPNGDV
                     AYVYLDPDYFLEIRTVSRRIEHGIPNETITDYGDYEKVNGVYLPFALESYEKGSSDHQ
                     KVQIEKAEANVAPDKSVFGFPPPRTAAVPAR"
BASE COUNT          354 a          681 c          635 g          378 t
ORIGIN      
        1 gcgtagtcat gatgcagatc gcgctcatcg gcccgcacgt ccgcgcggtc gcgctggatg
       61 tcctggcgat cgctcctgat gtcagcccgg tcgcgagcga tgtcgcgccg gttgctgctc
      121 acatcgtgac catcgcgacg ctcgtcgcca taccgatggt cacccgagcg ctgcccgtcc
      181 cggtcactcc attgctgctc gccggtctgc gcggcgccgg tattcggcgc ttgctggttg
      241 gccggctgat tgtcgggcac gggcgtccac tgttcatccg catatgcggt tgacaatgcc
      301 gcacccatga tcgccgcgac ggtgatagct atgaggttgt tgcgtttcat gacacctcct
      361 ttgtcgtctc gagctacgaa actactcgaa cgtcatcggt tctcgggcgc ctgcgcatcg
      421 ctgcgtactt ccgcatgacg ttcctcatgc ggcttgcgct catgcgcggc ccgctcctgc
      481 tcctggtcga tgtatgactg caccaacgcg aaaaatcgtg cgtagaacgc cgggtccttc
      541 agggtttcac gccgtaccac accgaggact tgtgccgagc ctgagggcat ggtcagggtt
      601 gccggcccaa ctccgacgct ggtcgactgc ttcatcttct gcagccggct gtcctcgcgc
      661 tccgccgtca cgaacacgat gctgctaccg tcgcggttgt ccgcgcaggt ggtctgcagg
      721 tgatagctca cgttcagctt cggatcgggc tggaagtccc tcgtgcccgt gagcaccccg
      781 gactcgcccg gccgatcgag catgtagccc tgactgagga gcgcgcgctt cacgctccag
      841 cagaccgcat cgcccgcgcc ttcgaaggtc ctgctgaaag gcgcctgcgt gctgatgctt
      901 tgctgcgcga catccacctt gcgtgaggcg cagccagcac acgtgagcgc caccgcaacg
      961 agcagcaccg ctcgataccg gttcgtcgtg ttggcggaac cgtgcacatt aacctccgtt
     1021 cccggctcac atccgagctg tgacctatgg gtgttggacc gcagcccgca cgggcgaagt
     1081 tccctgcggt ttcagggctg gaagggaggg tctaacgcgc tcgcgccacg aaaacccgcg
     1141 ggaatattct gacagtcaca tcggtcagcc aaatgagcgc tcgaccgact gacgagagaa
     1201 cggcgcttcg gtcccacagg cacgctgccg cacgcccggc ggctggcgcg cgcgcagcgt
     1261 tgaatgcatc atatgccgct ctcgaaagga cagggggatg catgcatcga atcgccgccg
     1321 ccctgatgct catggcggcc ccgggcgcgt tcgcgtacac cgcagccgag ctcgccgcca
     1381 agaatgtcgc cgccaaaggc ggcgccgaga agctcgatgc catccagtcg ctgcgcatgt
     1441 cgggcaagct gctgctgcgt ggcggcgcgg tccaggtcgg ctacaccatg ctggtggagc
     1501 gtccgcatgc gatccgctac gaagtgcagc tccagggtct cacgcgcgtg caggcctacg
     1561 acggttcgga ggcctggcag atcaatccct tccagggacg caaggatccc gagaaactct
     1621 cggccgacga tgccaaggcg ctggccgagg acgcggcaga tttcaccggc agcctggtcg
     1681 actatcaggc caagggttac cggctcgact atctgggcac cgaggacatc gatggaaccg
     1741 aggcgcataa gctgcgggtg acgcggccca acggcgatgt cgcctatgtc tacctcgatc
     1801 ccgactactt cctcgagatc cgtaccgtca gccgccgcat cgagcacggt atccccaacg
     1861 agaccatcac cgactacggt gactatgaga aggtgaacgg cgtctatctg cccttcgcgt
     1921 tggagtcgta cgagaaaggc tcgagcgatc atcagaaggt gcagatcgag aaggctgaag
     1981 ccaacgtcgc gccggacaag agcgtctttg gattcccgcc gccgcgaacg gctgccgtcc
     2041 ccgccagg
//
LOCUS       DATDMP010000069         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1567124, whole genome shotgun sequence.
ACCESSION   DATDMP010000069 DATDMP010000000
VERSION     DATDMP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567124"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..442
                     /locus_tag="VI195_01175"
     CDS             44..442
                     /locus_tag="VI195_01175"
                     /inference="COORDINATES: protein motif:HMM:NF008769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY6823023.1"
                     /translation="MAKPLPKRPYLLRAMHQWIAECGHTPHVIVDAGREGAEVPRAYV
                     KEGKIVLNLSEGATQRLHLGNEQVEFDARFAGVIHHVRFPVSAVLGIYARETGEGMVF
                     SEQDLGPEPPTRPPQTEEGGTRRPQLKVVK"
     gene            complement(505..1056)
                     /locus_tag="VI195_01180"
     CDS             complement(505..1056)
                     /locus_tag="VI195_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY6823024.1"
                     /translation="MLAAGAGALGLSLGAFAQDKEALETGANEDLMREHGILRRALFV
                     YAEASHRARIAPGTLPLPQLLATAQLFRSFGEEYHERQLEEQYIFPVVRRRDAQAAQF
                     VDVLHAQHQPGRDITAYIVRVAQRARLAPADAQPLAGALDGLVAMYGPHAAREDTDLF
                     PVWKKAIGARAYAEMASRWPSGC"
     gene            complement(1197..1573)
                     /gene="rnpB"
                     /locus_tag="VI195_01185"
     ncRNA           complement(1197..1573)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VI195_01185"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1643..>2215)
                     /locus_tag="VI195_01190"
     CDS             complement(1643..>2215)
                     /locus_tag="VI195_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6823025.1"
                     /translation="AAAGYEVSAIPGPSAITAALALAGLPTDRFCFEGFLPARARERR
                     AALGALARETRTLVLFEAPHRIVAALADMAAVLGAERAAVVARELTKAHERIYRGTLG
                     ELAARAATEPNFARGELTLVVRGAAAGAAGVDESLLRRAVEVLSKELPPARVAAVAAQ
                     LSGATRAAAYALVRRAGEDPSESTEETEAP"
BASE COUNT          343 a          765 c          752 g          355 t
ORIGIN      
        1 gctctttccg agcgcgagca ggagatgcgc ctgctgtaaa cccatggcca agcccttacc
       61 caagcgtccc tatctgctgc gcgccatgca ccagtggatc gccgagtgcg ggcacactcc
      121 gcacgtcatc gtggacgcgg gccgcgaagg ggccgaggtg ccgcgggctt atgtcaagga
      181 gggcaagatc gtgctcaacc tcagcgaggg cgccacgcag cgcctgcact tgggaaacga
      241 gcaggtcgag ttcgatgcgc gcttcgccgg ggtgatccac cacgtgcgct tcccggtgag
      301 cgcggtgctc ggcatctatg cgcgcgagac cggtgaaggc atggtgttct cggagcagga
      361 tctgggaccg gagccgccga cgcgaccgcc gcagaccgag gagggcggta cgcggcggcc
      421 gcagctgaag gtcgtcaagt aatcggcggc gttggccgcg cgcttctcat gcgggtggag
      481 gcggcgcggt gacctgcgcg aggttcagca gcccgaaggc cacctcgatg ccatctccgc
      541 gtaggccctg gcaccgatcg ccttcttcca gacagggaaa agatcggtgt cctcgcgcgc
      601 agcgtgcggg ccgtacatgg ccacgagccc atcgagcgcg cccgcgagcg gttgcgcgtc
      661 ggcgggtgcg aggcgagcgc gttgggcaac ccgcacgatg taagcagtga tgtcccggcc
      721 gggttggtgt tgggcatgca gcacatcgac gaattgcgcg gcctgagcgt cgcggcggcg
      781 cacgacgggg aagatgtact gctcttcgag ctggcgctcg tgatactcct cgccgaagct
      841 gcggaacagc tgcgcggtcg cgagcagctg cggcagtggc agcgtgccgg gcgcgatgcg
      901 tgcccggtgg ctcgcctcgg catagacgaa aagtgcgcga cgcaggattc cgtgctcgcg
      961 catgagatcc tcgttcgccc ccgtttcgag cgcctccttg tcctgtgcga aagcgccgag
     1021 cgacaggccg agtgcgccag caccagcagc cagcacgatc cggcgtcggt caatcagatg
     1081 gtccgtggat tcgctccttg aatccgcgcg cagcgcgtaa aacgcagctt gcgctcttcc
     1141 cacttgatga acgggcttcg ccgctgcgaa gcccgttgat cccgcgcgta gcgggtaaaa
     1201 agagtcggcc tgtaagccgg gttctgtcga gggcggtcat tcctctggga tccccgtcgc
     1261 cgtggatctc tagcagccta cccggaagcg cgcgcggatc ggcgctgcac gagccctgtg
     1321 ggcccgtgcg gcttccctat ttggccttgc tccaggtggg gtttgccgtg ccgtcgagtg
     1381 ttaccaccga cgcggtgcgc tcttaccgca ccgtttcacc cttgccggtc ccgcaaggga
     1441 cttaggcggt ctgctctctg ttgcactttc cgtgggctcg cgccccccag gcgttacctg
     1501 gcacccgatc cgccggagcc cggactttcc tccaccgaag cgaagcttca gcagcgactg
     1561 cccggccgac tctcaccagg caagatacgg cggctgggcg cgaagtcaaa ctcgagcgca
     1621 tccgccttga gcgcggctgc cgtcagggcg cttcggtctc ctcggtcgac tcgctcgggt
     1681 cctcgccggc gcgcctcacc aacgcgtaag ccgcggctcg cgtggcgccg ctcagttgcg
     1741 ctgccacggc ggcaacccgt gcgggcggca actctttcga caacacctcg accgcgcgcc
     1801 gtagcaatga ttcatcgacg ccggccgcgc cggccgccgc gccgcgcacg accagggtca
     1861 gctccccgcg cgcgaagttc ggctcggtcg ccgcgcgcgc cgccagctca ccgagcgttc
     1921 cgcggtaaat gcgctcgtgc gccttggtga gctcgcgcgc cacgaccgct gcgcgctccg
     1981 caccgagcac cgcagccata tcggcgagtg cggccacaat gcggtgcggc gcctcgaaca
     2041 acaccaacgt acgtgtctca cgggcgagcg cgccaagcgc tgcgcgacgc tcgcgtgcgc
     2101 gtgcaggcag aaagccttcg aagcagaaac ggtcggtcgg caaccccgcc agcgcgagcg
     2161 ctgcagtgat cgccgaggga cccggaatcg cgctcacctc gtagcccgcg gctgc
//
LOCUS       DATDMP010000070         4528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_233875, whole genome shotgun sequence.
ACCESSION   DATDMP010000070 DATDMP010000000
VERSION     DATDMP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233875"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1680)
                     /locus_tag="VI195_01195"
     CDS             complement(73..1680)
                     /locus_tag="VI195_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEY6823026.1"
                     /translation="MIAALLLASAMLSIESTDQGTGTPRNTPAAAEHKSVVVPPLPQT
                     APPAQPGPFTFADVQQLAHDRAAHDYRAPPQNLPTSLANLTYDQYRDIRFRPASALWR
                     GQALFEVQFFHRGYRVRQRVNVYEVTPAGAGLIEYSPRLFTFGRLMRTPKLSTEVGFA
                     GFRVHYPLQSPNYKDELLVFLGASYFRVLGRNQHYGASARGLAIDTAAQSGEEFPTFT
                     DFWLVRPQSSDRSLTIYALLDSRSVTGAYQFEVRPGEVTQVEVHCDLYPRRNIGKLGV
                     APLTSMYLYGEDGSGRRFDDFRPQVHDSDGLMNETGHGQWIWRPLANPRELRVNRFMD
                     EDPRGFGLLQRDRNFAHYQDVESQYQSRPSYWVQPLGNWGKGGVELVEIPSDEEIHDN
                     MVAYWVPDQAVAAGKPLSFAYLLSAFADNPRWPPGGRVVAVRSGNPAVGDNKGRFGPG
                     ARRMLVEFAGGELDGLDGSQPVKAELSADNGQIDALTVQHVPQSGSWRIAFVVTPRPK
                     KPVVDLRCYLTLYGEVLTETWVYQWSP"
     gene            2047..2580
                     /locus_tag="VI195_01200"
     CDS             2047..2580
                     /locus_tag="VI195_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY6823027.1"
                     /translation="MSNEEAIIQPFGSLVSSRLGLSEQVRHTSVAALNRLLAHTMAMR
                     DLYKKAHWQTSGATFYELHLLFDKHYGEQVELMDSVAERVQTLGGVTLALAQDVAEET
                     RIARAPRGRESVPAQLRRLLEAHETVLGEARPLAHEAAQAGDDGTNDLIVSEVIRTNE
                     LQSWFIGQHLMARGEKL"
     gene            2683..4317
                     /locus_tag="VI195_01205"
     CDS             2683..4317
                     /locus_tag="VI195_01205"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823028.1"
                     /translation="MAPETRNSTSTAAVHAAALPPWGALRYAVFRWLWIATVASNIGT
                     WMYNAASGWLMTSLDPSPLIVSLVQVANSLPLFLLALPAGALADMLDKRRLILALEIL
                     STLLSAVFAVLVMLHAVGPALLLLFTVLIGTLAALETPAWQAIVPLLVPPAALSSAIA
                     VNSVGINISRVVGPAITGVIILALGIAAPFWVDAFSNAGVIAVIFLWRPAARAVRPLP
                     AERLGGAIRAGVRYARYNKALRATFARAVGFFLFASAYWALLPLVARTQLKGGPGLYG
                     ALLAAIGAGAVGGALFLPRARHRAGPDGVVMLGEAGTVAALVLFGLAQQPWVAVLACV
                     LAGVSWISVLATLNVSAQTVLPDWVRGRGLAAYVTVFFGTMTAGSALWGFVAERVGLV
                     SSHYLAAAGALVALWITRRWRLHSGPAADLTPSMHWPEPVLAQRVDADAGPVLVTVEY
                     HVAAEHREAFLNAIVPLARERRRDGAYEWDIFEDSAHPERMIETWLVDSWVEHMRQHG
                     RVTRADRAIEERVRRLAREAPRITHYIAARPRRPPR"
     gene            complement(4318..>4528)
                     /locus_tag="VI195_01210"
     CDS             complement(4318..>4528)
                     /locus_tag="VI195_01210"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6823029.1"
                     /translation="IAGAEKVGHHKTSMLQDVEAGRAPELEALLGSVVELGRLTHTPT
                     PHIDAVYGLTHLLARTVANARPVAP"
BASE COUNT          721 a         1535 c         1506 g          766 t
ORIGIN      
        1 ccgaggtcag cacggtgagg ccgaagaaca gcgtgcgccg caggatcacc tgccagcggc
       61 ggcggcgctc gctcagggcg accattggta cacccaggtt tcggtcagga cctcaccgta
      121 gagcgtcaga taacagcgca aatccaccac cggtttcttc ggccgcgggg tgacgacgaa
      181 ggcgatgcgc caggagccgc tctggggcac gtgctgcacg gtgagcgcat cgatctggcc
      241 gttgtcggcg ctcagctccg ccttgaccgg ctgagagccg tcgagcccat cgagctcacc
      301 gccggcgaac tcgaccagca tgcgccgggc accggggcca aaacggcctt tgttgtcgcc
      361 caccgctgga tttccgctgc gcacggccac cacccgtccg cccggtggcc aacgcggatt
      421 gtccgcgaaa gccgacagca gataggcgaa gctcagcggc tttcctgccg ccaccgcctg
      481 gtccggcacc cagtaggcca ccatgttgtc gtgaatttcc tcatcgctcg ggatctccac
      541 gagctcgacc ccacctttgc cccagttgcc cagcggctgc acccagtaac tcggccgcga
      601 ctgatactgc gattcgacgt cctggtagtg cgcaaagttt cggtcgcgct ggagcaggcc
      661 aaagccgcgt ggatcctcgt ccatgaaacg attgacccgc agctcgcgtg gattcgcgag
      721 cggacgccag atccactgcc cgtgcccggt ctcgttcatg aggccgtcgc tgtcgtggac
      781 ctgcgggcgg aagtcatcga aacgccgccc gctgccatcc tcgccgtaga gatacatcga
      841 ggtgagcggc gccacgccga gcttgccgat gttgcggcgc ggatagagat cgcagtgcac
      901 ctcgacctgc gtgacctccc ccggccgcac ctcgaactga taggcccccg tgacgctgcg
      961 gctgtcgagc agcgcgtaga tcgtgaggct gcggtcgctc gactgcgggc gcaccagcca
     1021 gaagtcggtg aacgtcggga attcctcgcc actctgcgcg gcggtatcga tcgccaggcc
     1081 gcgcgcggat gcgccgtagt gctggttgcg gccgagcacg cggaaatagg aagcgccgag
     1141 gaacacgagc agctcatctt tgtagttggg cgactgcaat ggatagtgca cgcgaaaccc
     1201 ggcaaacccg acttccgtcg atagcttcgg tgtcctcatc aggcggccga aggtgaagag
     1261 tcgcgggctg tactcgatga gccccgcacc cgccggcgtc acctcgtaca cgttgacacg
     1321 ctggcgaacg cggtagccgc ggtgaaagaa ctgcacctcg aacagagcct ggccgcgcca
     1381 tagggcactc gccggacgga agcggatgtc gcggtactga tcataggtca ggttggcgag
     1441 cgatgtcggt aagttctgcg gtggcgcacg gtagtcgtgt gcggcgcgat cgtgtgccag
     1501 ctgctgcacg tcagcaaagg tgaacggccc gggctgcgct ggcggcgcgg tttgcggcag
     1561 cggcggcacg acaaccgact tgtgctcggc cgcagccggc gtgttgcgtg gggtccccgt
     1621 gccctgatcc gtcgactcga tcgacagcat ggccgatgcc agcaaaaggg ccgcaatcac
     1681 ggccggcttc cgcaggctcg gcggcatgaa tgcacctggt tattctccgc tggcagcaag
     1741 ccgggcctcg gcctgtttgc gaggagtctg ccagcgttag aaagacggct tctctgaatc
     1801 atctgtcgga gcaggctgac ataaggtatc gccgtgagcc acggttatta ggtatcgaag
     1861 gcggtcccgg gctcttagtt aggatactgc cagggccgcg ctcacgtctg cacccaaaca
     1921 cgcatcaaac acccccatca tgacaccgga tgaccctgcg ggggcactgt ctgcggcggc
     1981 cggattacac tgcgcgatca cgaaccggcc aatcgctccc cgccgcactt tccaaggaga
     2041 gaatcgatga gcaacgaaga agcgatcatc caacccttcg gcagcctcgt ctcgtcccgc
     2101 ctgggacttt ccgagcaggt ccgccacacg agcgtggccg cgctcaatcg cctgctcgcc
     2161 cacaccatgg caatgcgcga tctctacaag aaagcgcatt ggcagacctc cggtgccacc
     2221 ttctacgagc tgcacctgct gttcgacaag cattatggtg agcaggtcga gctcatggat
     2281 tccgtcgccg agcgggtgca gaccctcggt ggggtaaccc tcgccctcgc ccaggatgtc
     2341 gccgaggaga cccgcattgc gcgcgcgccg cgtgggcgcg agagcgttcc ggcgcagctg
     2401 cgccgcctgc tcgaggcgca cgaaaccgta ctcggcgagg cacggccgct cgcccatgaa
     2461 gccgcgcagg cgggcgatga cggcacgaac gatctgatcg tgagcgaggt catccgcacc
     2521 aacgagctgc agagctggtt catcggacag cacctgatgg cgcgcggcga gaagttatga
     2581 gttgattcgc ggcaggccgg cacgtgcaac tcgcggccgg ccggctgtgc tccgcgttgc
     2641 ctgagcaccg ccgcaaaggc ataggatgcc gggcgaggcg tcatggcacc ggagacccga
     2701 aactcgacct ctactgcggc ggtgcacgcg gcagcgttgc cgccgtgggg tgcgctgcgc
     2761 tacgcggtgt tccgctggct gtggatcgca accgtcgcat ccaacatcgg cacatggatg
     2821 tacaacgccg cctcggggtg gctcatgacg agcctcgatc cgagcccgct catcgtctca
     2881 ctggtgcagg tcgccaacag cctgccgctg ttcctcctcg cgttacccgc cggtgcgctc
     2941 gccgacatgc tcgataagcg ccggctgata ctcgccctcg agattctctc gacgctgctg
     3001 agcgcggtgt ttgcagtgct cgtcatgctg cacgccgtgg gccccgcgct gctgctgctc
     3061 tttaccgtgc tcatcgggac actcgccgcg ctggagacac ctgcgtggca ggcgattgtg
     3121 cccctgctcg tacctcccgc cgcgctctcc tccgcgatcg cggtcaacag cgtgggaatc
     3181 aacatcagcc gcgtcgtggg tcctgccatc accggtgtga tcatcctggc gctcggcatc
     3241 gccgcgccct tctgggtcga cgccttcagc aacgccggtg tgatcgccgt gattttcctg
     3301 tggcgcccgg cggcaagagc ggtgcgcccg ctgcccgccg agcggctcgg gggtgccatt
     3361 cgcgccggcg tgcgctacgc gcgctataac aaggcgctgc gagcgacctt cgcgcgtgcc
     3421 gtcggcttct tcctgttcgc gagcgcctac tgggcactcc tgccgctcgt ggcacgcacg
     3481 cagctcaagg gcgggcccgg cctctacggc gcactgctcg ccgcgatcgg cgcaggcgcg
     3541 gtggggggtg ccttgtttct gccgcgcgca cggcaccgcg cgggccccga tggcgtcgtg
     3601 atgctggggg aggcgggcac ggtcgcggcg ctggtgctct tcgggctcgc gcagcagccc
     3661 tgggtggcgg tgctcgcctg tgtgctcgcc ggcgtctcct ggatcagcgt actcgccacg
     3721 ctcaatgtct cggcacagac ggtgctgccc gactgggtgc gcggccgcgg actcgccgca
     3781 tacgtgacgg tgttcttcgg gaccatgacg gccggcagcg cactgtgggg ttttgtcgcc
     3841 gagcgtgtgg ggcttgtgtc ctcgcactac ctcgccgcag ccggcgcgtt ggtggcgctg
     3901 tggatcacgc gccgctggcg gctgcactcg ggcccggcgg cggacctgac cccatcgatg
     3961 cactggccag agccggtgtt ggcacagagg gtggatgccg atgcaggccc ggtgctggtc
     4021 acggtcgaat accacgtggc cgccgagcac cgcgaagcct ttctcaacgc catcgtgccg
     4081 ctcgcccgcg agcgacgccg cgatggagcg tacgagtggg acatattcga ggactcggca
     4141 caccccgagc ggatgatcga gacctggctg gtcgactcct gggtcgagca catgcgccag
     4201 cacggccggg ttacgcgcgc agaccgtgcc atcgaagagc gggtgcggcg cctcgcccgg
     4261 gaggcgccgc gcatcaccca ctacatcgcg gcgcgacctc ggaggccgcc gcgctgatca
     4321 gggtgcgacc ggccgggcat tcgccaccgt acgtgcgagc agatgggtca gtccgtagac
     4381 cgcatcgatg tgcggggtcg gggtatgcgt gagccgcccg agctcaacca ccgagcccag
     4441 cagcgcctcg agctccggcg cgcgccccgc ttcgacgtcc tgcaacatcg aggtcttgtg
     4501 atggccgacc ttctccgctc cggcgatg
//
LOCUS       DATDMP010000071         3296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_123505, whole genome shotgun sequence.
ACCESSION   DATDMP010000071 DATDMP010000000
VERSION     DATDMP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123505"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1002)
                     /locus_tag="VI195_01215"
     CDS             complement(262..1002)
                     /locus_tag="VI195_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6823030.1"
                     /translation="MALLILLYVIQAGLIIHVIKTGRNQLWIWVLLLLPAGIGALAYL
                     AVEIIPELLRSRTAQRTARGFRKALDPDADLRRYESEARIAGNVASRQRYAEELARHE
                     RFDEAIAQYRQSLSGLYEHDPNLMLGLAQAQFAKGDAPGARTTLDELIRLNPDFRSPV
                     GHLLYARALEAEGNVAKALEEYRVVAGSYPGAEAAVRYAQLLQAQGQRAEAQRIVREL
                     LEQARIAPGHYRRAQRSWLEAAQRLLQS"
     gene            complement(1022..3052)
                     /locus_tag="VI195_01220"
     CDS             complement(1022..3052)
                     /locus_tag="VI195_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEY6823031.1"
                     /translation="MNAYGPTPPPSRTCRALRPRLRARALCTFAACSLAGLPLAALAQ
                     APGSSDWGYYGGDALGQHFSSLDQINRGNVTRLARVWTYRTGELGSGFASAPRLTFEA
                     TPVLAFGLLYLETASNVVIALDPESGRERWRFDPHIDRTRRYAEASARGVSLWSATAL
                     AGSGACRHRVFTGTLDARLLALDADNGRLCSDFGDGGQVDLTRGLRIRDAGAYLVTSP
                     PAIFGRVVIVGSAIGDNRAAQVERGVIRAYDAESGAQLWAFDPLPDSALHPAAGEWSL
                     EESHSSGAANAWGVMTIDQEHGLVLVPTGSASPDYYGGQRHGANRFADALLALDARSG
                     RLVWQQQLVHHDLWDYDLAAQPVLGDVEVQGIPVPAVIQATKTGMLYVFERARGGALF
                     PVTERSVPVSRVPGENAWPTQPFSSLPPLTPQRAVVSDDAWGLTFWDRSKCRALIATL
                     RNEGIFTPPDLRGTLLVPGYIGGVNWGGLAFDQDHARIIAAVNLLPMVVTLLTRAEFE
                     REVHSGAYPHSQFGPQAGTPYGMRREPLLSPFGLPCTAPPWGVLVSVDLRHQRIAWQS
                     PLGSTEGFTPWFLPVREFGMPNMGGPIVTAGGLVFVGAAMDGYLRAFDIETGAELWKH
                     KLPAGGQATPMTYRAGASQRQYLVISAGGHGPLGTPRGDYVVAFALPAARAP"
BASE COUNT          539 a         1126 c         1130 g          501 t
ORIGIN      
        1 acccaacaac agtgccagcg cggggggcgg gggtagtcac ggcggcggtg gcgcgctcga
       61 gccgctgacg ctgctggcac tcgccggccg tgtactcagc gcccggcgca cgagactcag
      121 gcagcgcgtg aagagatcgg ccggttgacc atttcttgag gcgcgcgctg ttatttttct
      181 ctggcgcgat agttaaccag ttggttaacc attgaggcag acagcccgag cgagtctccc
      241 actgcggttg atcactccgc cctaggactg cagcagccgc tgcgcggcct cgagccagct
      301 gcgctgcgcg cggcgatagt gtccgggtgc gatgcgcgct tgctcgagca gctcgcgcac
      361 gatgcgctgc gcctcggcac gctgtccctg cgcctgcaac agctgcgcat agcgcaccgc
      421 cgcctcggcg cccggataag agccagcgac cacgcgatac tcctccagcg ctttcgccac
      481 gttgccctcg gcctcgagcg cgcgtgcata caacaggtgt cccaccggcg agcggaagtc
      541 ggggttgaga cggatcagct catcgagcgt ggtgcgggcg ccgggcgcat cgcccttggc
      601 gaactgcgcc tgcgcgagtc ccagcatcag gttaggatcg tgctcgtaca ggccgctgag
      661 actctggcgg tactgggcga tggcctcatc gaaacgctcg tggcgcgcga gctcctccgc
      721 gtagcgctgg cgactcgcga cgttgccggc gatgcgcgcc tcgctctcgt agcggcgcag
      781 atcggcatcg gggtccaatg ctttgcggaa accgcgcgcg gtgcgttgcg cggtgcggct
      841 gcgcagtagc tcggggatga tctccaccgc caggtaggcg agcgccccga ttcccgccgg
      901 cagcaacagc agcacccata tccacagttg gtttcgcccg gtcttgatga catggatgat
      961 gaggcccgcc tggataacgt acagtaagat cagcaatgcc attccagctc tctccgctaa
     1021 ctcacggcgc ccgcgcggcg gggagcgcga aggcgaccac gtagtcaccg cgcggcgtcc
     1081 cgagcggtcc gtgtccaccg gcgctgatga ccaggtactg cctttggctc gccccggcac
     1141 gataggtcat cggggtcgcc tgaccgccgg cgggcagttt atgtttccac agctctgcgc
     1201 cggtctcgat gtcgaaggcg cgcaggtagc cgtccatcgc ggcaccgacg aacaccaacc
     1261 cgccggcggt cacgatcgga ccgcccatgt tgggcatgcc gaactcccgc actggcagga
     1321 accatggggt gaacccttcg gtcgagccaa gcggtgattg ccaggcgatg cgctggtgcc
     1381 tgagatcgac actcaccagc acgccccagg gcggtgccgt gcacggcagt ccgaaaggcg
     1441 agagcagcgg ctcacggcgc atgccatagg gtgtgcccgc ttgcggtccg aactgcgaat
     1501 gcgggtacgc gcccgagtgc acctcgcgct cgaactcggc acgcgtgagc agcgtcacca
     1561 ccatgggcag caggttcacc gcggcgatga tgcgcgcatg gtcctgatcg aaggccaggc
     1621 cgccccagtt caccccaccg atatagccgg ggacgagcag cgtaccgcgc aggtcgggtg
     1681 gggtgaatat cccttcgttg cgcagcgtcg cgatgagcgc gcgacacttg ctgcgatccc
     1741 agaaggtgag accccaggca tcgtcggaga ccacggccct ctgcggggtg agcggcggga
     1801 gggatgaaaa gggttgcgtc ggccaggcat tctcgcccgg cacgcggctc accggcacgg
     1861 agcgctccgt gaccggaaac agagctccgc cgcgcgcgcg ctcgaacacg tagagcatgc
     1921 cggtcttggt cgcctggatc acggccggca ccggtatgcc ctgaacctcc acatcgccga
     1981 gcaccggttg ggcggcgagg tcatagtccc agaggtcgtg atgaacgagc tgctgttgcc
     2041 acacgagccg gccgctgcgc gcatcgaggg caagcagcgc atcggcgaag cgattggcgc
     2101 cgtggcgctg gccaccataa taatcgggac tcgccgagcc ggtcggcacg agcaccagac
     2161 cgtgctcctg atcgatggtc atgacccccc aggcgttggc ggcgccactg ctgtgcgact
     2221 cctcgagact ccactcgccc gccgccggat gcagggccga gtcgggcagc gggtcgaagg
     2281 cccagagctg tgccccgctc tcagcgtcgt acgcacgaat gacgccgcgc tcgacctgcg
     2341 ccgcacggtt gtcgccgatt gccgagccga cgatcacgac cctgccgaag atcgccggcg
     2401 gggaggtgac cagatacgcg ccggcatcgc gaatgcgcag gccacgggtg agatccacct
     2461 gaccgccgtc gccgaaatcg ctgcacaggc ggccgttgtc ggcatcgagc gcgagcagac
     2521 gcgcatcgag tgtgccggtg aacacccggt gacggcacgc accgctgcct gccaaagcag
     2581 tggcgctcca taggctgacg ccgcgcgcgc tcgcctccgc atagcggcgc gtgcgatcga
     2641 tgtgcgggtc gaagcgccag cgctcccggc ccgactccgg gtcgagcgcg atcacgacgt
     2701 tgctggccgt ctcgaggtaa agcaggccga aggcgagcac cggggtcgct tcgaaggtga
     2761 gccgtggggc gctggcgaat ccgctgccga gctcaccggt gcgataagtc cacacccgcg
     2821 caaggcgcgt gacgttgccg cggttgatct gatcgaggct ggagaagtgc tggcccaacg
     2881 catcgccacc gtagtacccc cagtcgctcg aacccggcgc ctgcgcgagt gcagcgagcg
     2941 gcaggccagc cagcgagcag gccgcgaacg tgcacagcgc ccgtgcgcgc agccgggggc
     3001 gcagcgcgcg gcaggtacgg ctcggcggcg gcgtcgggcc atatgcattc atgcccgcat
     3061 tatctgtgat cgcgtccgcg ggctgcgagc cccgaggcgc gccgcgcagg acaatgctgg
     3121 tattcttcgg tgcgtgagtt caacacgggc ggtcgcgtcc cgtcttcggg cggtgagtct
     3181 attcctggca gcttggctcg cggtgcccgc cctcaccgac gcacaggcaa cctccgagcc
     3241 gccgagctca cacgcgccgc accatcgccc gcgcatcggg ctggtgctct cgggcg
//
LOCUS       DATDMP010000072         2687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1678923, whole genome shotgun sequence.
ACCESSION   DATDMP010000072 DATDMP010000000
VERSION     DATDMP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678923"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VI195_01225"
     CDS             <1..808
                     /locus_tag="VI195_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY6823032.1"
                     /translation="GEETFALCRSHVDEIVLTSTDEICAAIQDVFEDTRSIVEPAGAL
                     AVAGLKQYLARQGWRERRVVAIASGANMNFDRLRHVAERADLGGEREALLAVVIPEQP
                     GSFLRFCEALGRRSVTEFNYRYASAAAAQIFVGFALAGGRAEKEAVVAQLHAAGFAVT
                     DMTDDEMAKLHVRYMVGGHAHGIEHELLYRFEFPERPGALLRFLQAIGSRWNISLFHY
                     RNHGSDYGRVLAGIQVPPAERADFLGHLNALHYAYVDETANPAYRMFLSA"
     gene            complement(926..2011)
                     /locus_tag="VI195_01230"
     CDS             complement(926..2011)
                     /locus_tag="VI195_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY6823033.1"
                     /translation="MSNPEGGRLEDFASAPWLRRLIMVVVLAGLVLLGFRVMEPFIVP
                     LVWAAILAFVTWPGYEWLIAKLRGRRTIAATLMTGAVTVAVIAPLAWLAVVLRLEIVH
                     VYHEGQRLLAGGAQLPPAVLRIPWLGDQLHELLTRAAQDPHALGLQFRQLTDRSFDEI
                     TRVIGGITRNVAKLLLAVISLFFAYRDGERFATQLARALEQVLGARVHNYLQAIAQTV
                     KAVVYGLVLAALFQGTLAGIGYWLAGTGAPVFLAALTTVFGLIPFAAPTLWGGLGVWL
                     VLTGKTVAGVALLVWGAIVVGSTDHMVRPLLISREAEIPFLVVMFGVLGGLAAFGLVG
                     LFVGPVLLAVLLAIWREWLLEHRPPPA"
     gene            complement(2008..2484)
                     /locus_tag="VI195_01235"
     CDS             complement(2008..2484)
                     /locus_tag="VI195_01235"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HEY6823034.1"
                     /translation="MKPLEDHAAPARLSTATELLGYLGIAPLLTCLVCVVLLPGYGGR
                     ELAQRGAIAWGAVLLAFTGAVHWGLTFAGRLPSHPVRTTAAALPAVVGAAAVLAGGQR
                     ALALLVVGFGLFWLYEHRSLGTQLPPAYLGLRRNLSLATCTLLALIMFASDAAGLL"
     regulatory      2570..>2687
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          460 a          942 c          956 g          329 t
ORIGIN      
        1 gggcgaggaa accttcgccc tgtgccgcag ccacgtcgac gagatcgtgc tcaccagcac
       61 cgacgagatc tgcgccgcga tccaggatgt gttcgaggac acgcgctcga tcgtcgagcc
      121 ggccggcgcg ctcgcggtgg cggggctgaa gcaatacctc gcgcgccagg gctggcgtga
      181 gcggcgggtg gtggcgatcg caagcggcgc caacatgaac ttcgaccgcc tgcggcacgt
      241 ggccgagcgg gccgatctcg gcggcgagcg cgaggcgctg ctcgcggtgg tgatcccgga
      301 gcagcccggc agcttcctgc gcttctgcga agcgctcgga cggcgcagcg tcaccgagtt
      361 caactaccgc tacgcgagcg ccgcggccgc gcagatcttc gtcggcttcg cgctcgccgg
      421 gggacgggcg gagaaggaag cggtggtggc gcagctgcac gccgccggct tcgcggtcac
      481 cgatatgacc gacgacgaga tggcgaagct gcacgtgcgc tacatggtgg gcggccacgc
      541 ccacggcatc gagcacgagc tgctctaccg gttcgagttt cccgagcgcc cgggcgcgct
      601 gctcaggttc ctgcaggcga tcggctcgcg ctggaacatc agcctgttcc actaccgcaa
      661 ccacggctcc gactacgggc gggtgctcgc cggcatccag gtgcccccgg ccgagcgggc
      721 ggacttcctc ggccacctca acgcgctgca ctacgcctac gtggacgaga ccgcgaatcc
      781 cgcctaccgc atgttcctgt ccgcctagtc tattgggcga aatcgcagga agcgatttcg
      841 ccggacatca ccctgggtgc cgccggcaaa aggcgtggct tttgccggct gggcatcagg
      901 gagcgatttc gccggacatc acaaactacg ccggcggcgg acggtgctcg agcagccact
      961 cgcgccagat cgccagcagc accgcgagca gcacgggacc cacgaacagg cccaccagcc
     1021 cgaaggcggc gagtcccccg agcacgccga acatcaccac caggaacggg atctccgctt
     1081 cgcggctgat gagcagcggg cgcaccatgt ggtcggtcga accgacgacg atcgcgcccc
     1141 acacgagcag cgcgaccccc gctacggtct tgccggtgag caccagccac acgccgagcc
     1201 cgccccagag cgtgggggcc gcgaacggta tcaggccgaa cacggtggtg agggcggcga
     1261 ggaacaccgg cgcgccggtg ccggcgagcc agtagccgat gccggcgagc gttccctgga
     1321 agagcgccgc cagcaccagg ccgtagacca ccgccttgac cgtctgcgcg atggcctgca
     1381 gatagttgtg gacgcgcgct cccagtacct gctcgagggc acgcgccagt tgcgtcgcga
     1441 agcgttcccc gtcacggtag gcgaagaaca ggctgatgac ggcgagcagc agcttggcga
     1501 cgttgcgggt gatgccgccg atcacgcgcg tgatctcgtc gaaggagcgg tcggtgagct
     1561 ggcggaactg caggccgagg gcgtgcggat cctgtgccgc gcgcgtcagc agctcgtgca
     1621 gctgatcgcc gagccacggg atcctcagca ccgcgggcgg cagctgcgcg ccgcccgcga
     1681 gcagccgctg accctcgtga tacacatgca cgatctcgag gcgcagcacc actgcgagcc
     1741 aggcgagcgg cgcgatgacc gccacggtca cggcgccagt catcagcgtg gcggcgatgg
     1801 tgcgccgtcc gcgcagcttc gcgatcagcc actcgtaacc tggccaggtg acgaaggcga
     1861 ggatcgccgc ccacaccagc ggcacgatga acggctccat cacgcggaag ccgagcagca
     1921 ccagcccggc gagtaccacc accatgatga ggcgccgcag ccagggcgcg gatgcgaagt
     1981 cctcgagccg cccgccctcc gggttgctca caggagtccc gcggcgtcgg aggcgaacat
     2041 gatgagcgcg agcagcgtgc aggtcgcgag gctcaggttg cgccgcagac cgaggtaggc
     2101 gggcggcagt tgagtgccga gcgagcggtg ctcgtacagc cagaagagcc cgaagcccac
     2161 cacgagcagc gcgagcgccc gctgtccccc ggcgaggacc gccgccgctc cgaccaccgc
     2221 cggcagcgcc gccgcggtgg tgcggaccgg gtgggacggc agccggccgg cgaaggtgag
     2281 tccccagtgc accgcgccgg tgaaggcgag cagcaccgcg ccccaggcga tcgcgccgcg
     2341 ctgcgcgagc tcacgcccgc cgtagccggg cagcagcacg acgcagacca ggcacgtcag
     2401 cagcggcgcg atcccgaggt agccgaggag ctccgtggcg gtgctcaggc gggcaggggc
     2461 agcatgatct tccaggggct tcacggcccg cgatcatgcc acgcgcggcg tcggggccgg
     2521 acatcttgac gacctgcaag ccgcgcaggc acccttcgcg cggcgtgagg tgctcgtacc
     2581 gctgcccggc ctcgcaacgc gaggcggcgc acgggaacgg gtaaacggga agtccggtgc
     2641 ggattcgtcc aaagccggcg ctgcccccgc aacggtaatc gagatca
//
LOCUS       DATDMP010000073         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1567976, whole genome shotgun sequence.
ACCESSION   DATDMP010000073 DATDMP010000000
VERSION     DATDMP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567976"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VI195_01240"
     CDS             complement(<1..528)
                     /locus_tag="VI195_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6823035.1"
                     /translation="MLETAPKSAESKGTTTAEDTLKRKSRDADVISGGHLVAKALKNE
                     GVDTIFTLCGGHIIDIYDGCIDEGIRIIDVRHEQVAAHAADGYARQTGKLGCVVTTAG
                     PGCTNAVTGIATAFRSESPVLHIGGQGALTQHKMGSLQDLPHVDMMSPITKFAATVPS
                     TERVADMVSMAAREAF"
     gene            complement(673..1983)
                     /gene="oxlT"
                     /locus_tag="VI195_01245"
     CDS             complement(673..1983)
                     /gene="oxlT"
                     /locus_tag="VI195_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEY6823036.1"
                     /translation="MATDTIPRPASSSALGQPWVQLCVGLVCMIMVANYQYGWTLFVG
                     PIGDKYGWSKAALQVTFTLFVLFETWLVPAEGWLVDKFGPRWMIVSGGVLVLFSWLCM
                     SFASSLGMFYFAGIVGGIGAGAVYGTCIGNAVKWFPGRRGLAAGLTAAGFGMGSALTI
                     IPIANMIKTSGYEHTFFTFALIQGIVTIIFGFFIMAPPATLFTAAPKIAPGASRYQAS
                     PTQMLSTPVFWIMYLMFVMMAAGGLMATAQLAPIAKDFHIDKVPVSILGLTLPALTFA
                     LTLDRILNGLTRPFFGWVSDQIGREQTMFIAFLLEGIGIIALANLGTNPVWFVVLSGL
                     VFFAWGEIYSLFPSTVTDTYGATYATTNTGLMYTAKGTASLLVPLTSVIAAKGNWHPV
                     FMMAATLNIIAAFMAVVLLKPMRSAYTSRLSGATVDPAAKLATR"
     gene            2484..3203
                     /locus_tag="VI195_01250"
     CDS             2484..3203
                     /locus_tag="VI195_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY6823037.1"
                     /translation="MSASVTPEGSIRVSGQLALSPLNSMSLRDQAYALLKEAIASTDI
                     YSQRQSLRLDERELMQALGVSRTPIREALSILEQEGFVRTLPRRGIFIVRKSKREIIE
                     MIQMWAALESMSARLATLHAPDTELAKLRHLFDEFQNSPPAEHIGEYSDANIAFHQMI
                     IRLGGSQLIADATKNLFIHVRAIRKATISQDNRAARSIIDHLKIIEALERRDTELAER
                     LTRQHTLDLAAHVERYCDFLD"
     gene            complement(3200..>3504)
                     /locus_tag="VI195_01255"
     CDS             complement(3200..>3504)
                     /locus_tag="VI195_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6823038.1"
                     /translation="LAFLSAHTISLDLKAGNLVVLDVQGFPALLNWYLVHNRTKRLPP
                     VASAFKEFLLREGAPRIERLVQFGFNSWPAEPRAHAPATASAAPTRTARRRSVRRT"
BASE COUNT          685 a         1092 c         1109 g          618 t
ORIGIN      
        1 gaaggcctcg cgcgccgcca tcgacaccat gtcggccacg cgctcggtgc tcggtaccgt
       61 cgccgcgaac ttggtgatcg gcgacatcat gtcgacgtgc ggcagatcct ggagcgagcc
      121 catcttgtgc tgggtgagcg cgccctgacc gccgatgtgc agcaccgggc tctcggagcg
      181 gaaggcagtc gcgatgccgg tcaccgcgtt ggtgcagccg gggccggcgg tcgtgaccac
      241 gcaaccgagc ttgccagtct ggcgcgcata gccatcggcg gcgtgcgccg ccacctgctc
      301 gtggcgaacg tcgatgatgc ggatgccttc atcgatgcag ccgtcgtaga tgtcgatgat
      361 gtggccgccg cagagcgtga agatcgtgtc gacgccttcg ttcttgagcg ccttggcgac
      421 caggtggccg ccggagatga catcggcgtc gcggctcttg cgcttgagtg tgtcttccgc
      481 ggtcgtcgta cccttgcttt ccgcagactt gggcgccgtt tcgagcatat ccgcctccat
      541 tctcaattca tgtctgcgag cgcaggctac ctcgtcgcag ctgcgcgcca gggaaaggcc
      601 tggacaggtg attccgcgcg caccgcgcgc tccccggacc gcagggctcg gaggagcgcg
      661 ctcgggcacg catcaacgcg ttgccaactt cgctgccggg tcaaccgtcg cgccgctgag
      721 ccggctcgtg tacgcgctac gcatcggctt gagtaacacg acggccatga acgcggcgat
      781 gatgttgagc gtcgccgcca tcatgaatac cgggtgccag ttgcccttcg ccgcgatcac
      841 gctggtaagc ggcacgagca gcgacgccgt gcccttggcc gtgtacatga ggcccgtgtt
      901 ggtggtcgcg taggtggccc cgtaggtgtc ggtcaccgtc gagggaaaaa gactgtagat
      961 ctcgccccag gcgaagaaca ccagacccga gagcacgacg aaccagaccg ggttggtgcc
     1021 gagatttgcg agtgcgatga tgccgatgcc ctccaacagg aaggcgatga acatcgtctg
     1081 ttcgcgcccg atctggtcgg acacccagcc gaagaacggc cgggtcagcc cgttcaggat
     1141 gcgatcgaga gttagcgcaa aggtgagcgc cggcaaggtc aggccgagga tactcaccgg
     1201 gaccttgtcg atgtggaagt ccttcgcaat gggtgcgagc tgggcggtcg ccatgagtcc
     1261 gcctgctgcc atcatcacga acatcaggta catgatccag aacaccgggg tcgacagcat
     1321 ttgtgtcggt gacgcctgat agcggctcgc ccccggggcg atctttggag ccgccgtgaa
     1381 gagagtggca ggcggcgcca tgatgaaaaa cccgaatatg atcgtcacga tgccctggat
     1441 gagggcgaag gtgaagaagg tgtgctcgta acccgaggtc ttgatcatgt tggcgatcgg
     1501 aatgatggtg agcgccgagc ccatcccgaa gcccgccgcg gtcagtcccg cggcgagtcc
     1561 acgccgtccg ggaaaccatt tgaccgcatt cccgatgcac gtgccgtaca ccgcgcccgc
     1621 gccgataccg ccgacgatcc cggcgaaata gaacatgccg agcgaactcg cgaaggacat
     1681 gcacagccag gagaacagga cgaggacccc gccgctcacg atcatccacc gcgggccgaa
     1741 cttatcgacc agccagccct ccgcgggtac cagccacgtc tcgaacagaa cgaacagagt
     1801 gaacgtgacc tgtaaagccg ccttgctcca accgtactta tcgccaatcg ggcctacgaa
     1861 aagcgtccag ccgtattggt agttggcaac catgatcatg cacacgaggc caacgcaaag
     1921 ttgcacccac ggttggccca aggctgaaga agaagcggga cgaggaatcg tatcggtagc
     1981 catcgctgtt tccccctaag ccgactgaag ccgagatgtc gtcaaccagt tgctctgagc
     2041 ctcaacgatc tgcactgcgc ttcgcatcgc cgcgcctgcc ttgcaccgcg tgcgtgcacg
     2101 acgcgctcgc tgaatgcgct gcggcggtgc gagctggcga agctctcccc aaaaagattt
     2161 cctgccgggt gacccctatg atttgtaatt aaatgattga ggctcatacc caaggtgact
     2221 actggtccaa catatgtgat atatctcagg cttgtcaaat aagtggtagt gagccttagc
     2281 ggggcggttt agctggggaa atcagctgcg tgctggcgcc tgcgggcgac agctcacgaa
     2341 atctgcacca agacggggag gcaggtcgtc cggtgcatat aagatatgtg atacatctaa
     2401 actgggaagt ggtcggggct tttgctcagt gactccattc ccgtgatgac ctgcgactcc
     2461 aggctgagca tgatggagag taaatgagcg cctccgtgac tcctgaagga agcattcgcg
     2521 tcagcggaca gctcgcgctt tcgccgctca actcgatgag cctgcgcgat caggcgtacg
     2581 cgctgctcaa ggaagcgatc gccagcaccg acatctacag ccagcggcag tcactgcgac
     2641 tcgatgagcg cgaattgatg caggcgctcg gggtaagccg cacgccgatc cgcgaggcgc
     2701 tctcgatcct cgagcaggag ggcttcgtgc gcacgctgcc gcggcgcggc atcttcatcg
     2761 tgcgcaagtc caagcgcgag atcatcgaga tgatccagat gtgggccgcg ctcgagagca
     2821 tgtccgcgcg cctcgcgaca ctgcatgcgc cggatacgga gcttgccaag ctccggcacc
     2881 tgttcgatga gttccagaac tcgccgcccg cggagcatat tggcgagtac tcggacgcca
     2941 acatcgcctt ccaccagatg atcatccggc tcggcggctc acagctgatc gcggatgcga
     3001 ccaagaacct cttcattcac gtgcgtgcca tccgcaaggc gacgatctcc caggacaacc
     3061 gcgccgcccg ttcgatcatc gatcatttga agatcatcga ggcgctggag cggcgcgaca
     3121 ccgagctcgc cgagcggctc acgcggcagc acacactcga tctggccgcg cacgtcgagc
     3181 gttactgcga ctttctcgat taagtccggc gtaccgatct gcgtcgcgcg gtgcgtgtcg
     3241 gcgccgcgct cgccgtcgcc ggcgcatgtg cgcgcggctc ggccggccat gagttgaagc
     3301 cgaactgcac gagccgctcg atgcgcggcg caccctcacg cagcaggaat tccttgaacg
     3361 ccgaggctac cggcggcagg cgtttggtgc ggttgtgcac cagataccag ttgaggagcg
     3421 cgggaaagcc ctgcacatcg agcaccacca agttacccgc cttgagatcg aggctgatgg
     3481 tgtgggcgga gaggaacgcg agcc
//
LOCUS       DATDMP010000074         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1234664, whole genome shotgun sequence.
ACCESSION   DATDMP010000074 DATDMP010000000
VERSION     DATDMP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234664"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VI195_01260"
     CDS             <1..888
                     /locus_tag="VI195_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6823039.1"
                     /translation="TGHTNYDVVVPSGAFLERQIEAGVYQKLDKALLPNLRNVDPEVA
                     RAVALYDPGNQYSVDYMWITSGVGYNTAEIRSRMPDAPVDSWRMLFDPAVVSKFQDCG
                     VSVLDAPSEVVATVLLYIGRNPGSNSPADLKAAEAVLKTIRPYVRYVDSSRYIDNLAN
                     GDICLVMGWSGDVKQAHDRARDAGKGVNLAYSIPGEGAISNYDVLAIPADAPHVRNAH
                     LFINFLLRPDIAARNSNLIKYANSVLPSIQPLDPAVAGDPGVYPPPQVRARLTPERAR
                     PAAYQRLLTRAWTRFKTGR"
     gene            901..2052
                     /locus_tag="VI195_01265"
     CDS             901..2052
                     /locus_tag="VI195_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6823040.1"
                     /translation="MSTSIEPRAADRAAAAADSYVRIRNLTRKFGDFVAVDDVSLDIR
                     RGEIFCLLGGSGSGKTTLLRMLAGFETPTAGSIEIDGQSLSAVPPYERPVNMMFQSYA
                     LFPHMSVERNVAFGLEQERLSRAEIRRQVGEILEIVRMRGFEARKPHQLSGGQRQRVA
                     LARALVKRPKLLLLDEPLAALDKKLREHTQFELLNIQQRLGVTFIVVTHDQEEAMTLG
                     TRLGVMDRGRIVQVGTPADIYESPATRFVADFIGSVNMFEGEVSESGAGAVSIRCAEL
                     GCTVRAGRGAPLARGATVWTAIRPEKINIARQAPAAQEPARPGNLVRGTVKEIAYMGD
                     MSIYLVRIGSGRLLRVTLPNIMRGAERPIAREETVWLSWHDSSPVVLTE"
     gene            2058..2951
                     /locus_tag="VI195_01270"
     CDS             2058..2951
                     /locus_tag="VI195_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6823041.1"
                     /translation="MRWSRAAGRGLVAGIPTLWLLLLFLVPFIIVFRISFSEVRLAIP
                     PYTPLIAWHHGEPALELHGSAYAFLFTDPLYLASYLYSLKVAAVSTLCCLALGYPMAY
                     AIARAGPATRPLLLMLIVLPFWTSFLLRVYAWIGLLNNNGVINNVLLYLGLIHRPLHL
                     LQTDFAVYIGIVYSYLPFMILPLYANLEKHDLSLLEAAADLGARPWRAFLRITLPQSL
                     PGIAAGALLVFIPAVGEYVIPTLLGRTDQLMIGRVLSDEFFENRDWPVASAVAILILL
                     LLIVPILLFQRFQQRQLEASR"
     gene            2948..>3508
                     /locus_tag="VI195_01275"
     CDS             2948..>3508
                     /locus_tag="VI195_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823042.1"
                     /translation="MTSRGLLTRAALLLGLVFLYVPIISMIVFSFNNSRLVSVWDAAH
                     SPTLKWYAALARNEQILGAAWLSIRIALISASAAVLLGTLAGVALARFGAYRGRLLLA
                     GLIPAPIVMPEVITGLSLLLLFVALGQLIGWPRGVGAVTITLAHITFCMAYVTVVVQS
                     RLAGFDESLEEAAMDLGARPLRVFLRI"
BASE COUNT          504 a         1249 c         1159 g          596 t
ORIGIN      
        1 accggccaca cgaactacga cgtggtggta ccctccggcg ccttcctcga gcgccagatc
       61 gaagcgggcg tctaccagaa gctcgacaag gcgctgctgc cgaatctgag gaacgtcgat
      121 ccggaggtcg cgcgtgcggt cgcgctctac gaccccggta accagtacag cgtcgactac
      181 atgtggatca cctcgggcgt gggttacaac accgccgaga tccgcagccg catgcccgat
      241 gcgccggtcg acagctggcg catgctgttc gatccggcgg tggtgtcgaa attccaggac
      301 tgcggggtgt cggtcctcga tgcgccctcg gaggtggtcg ccacggtgct gctgtacatc
      361 ggccgcaacc ccggcagcaa ctcccccgcg gatctcaagg cggccgaagc ggtgctgaag
      421 acgatccgcc cctacgtgcg ctacgtcgac tcctcgcgct acatcgacaa cctcgccaac
      481 ggcgacatct gcctggtgat gggctggtcg ggcgacgtca agcaggcgca cgaccgcgcc
      541 cgcgacgccg gcaagggggt gaacctcgcc tactcgatcc cgggcgaagg cgccatcagc
      601 aactacgacg tgctggcgat cccggcggac gcgccgcacg tgcgcaatgc gcacctgttc
      661 atcaatttcc tgctgcgccc ggacattgcc gcgcgcaatt cgaacctcat caagtacgcc
      721 aacagcgtgc tgccgtcgat ccagccgctc gaccccgcgg tggcgggcga cccgggcgtc
      781 tacccgcccc cgcaggtacg cgcccgcctg acgcccgagc gcgcccggcc ggccgcctac
      841 cagcggctgc tcacgcgcgc ctggacgcgc ttcaagaccg gcaggtaagg tcggcactcc
      901 gtgagtacca gcatcgagcc ccgggccgcc gatcgcgccg ccgccgccgc cgacagctac
      961 gtacgcatca ggaacctcac caggaaattc ggcgacttcg tcgcggtcga cgacgtatcg
     1021 ctcgacatcc gccgcggcga gatcttctgc ctgctcggcg gctcggggtc gggcaagacg
     1081 acgctgctgc gcatgctggc cggcttcgag acccccaccg ccgggagcat cgagatcgac
     1141 ggccagagcc tgagtgccgt accgccgtac gagcgcccgg tcaacatgat gttccagtcc
     1201 tacgcgctgt tcccgcacat gagcgtcgag aggaacgtgg cgttcggact cgagcaggag
     1261 cgcctgagcc gcgccgagat ccgccgccag gtgggcgaga tcctcgagat cgtcaggatg
     1321 cgcggcttcg aagcgcgcaa gccgcatcag ctgtccggcg gccagcgcca gcgcgtcgcc
     1381 ctggcccggg cgctggtgaa gcgcccgaag ctgctgctgc tcgacgagcc gctcgcggcg
     1441 ctcgacaaga agctgcgcga gcacacccag ttcgagctgc tcaacatcca gcagcgcctc
     1501 ggggtcacct tcatcgtggt cacccacgac caggaggagg cgatgaccct cggcacgcgg
     1561 ctcggagtca tggaccgggg ccgtatcgtg caggtgggga caccggcgga catctacgag
     1621 tcgccggcga cgcgcttcgt cgccgacttc atcggctcgg tcaacatgtt cgagggcgag
     1681 gtgagcgaga gcggcgcggg ggccgtgagc atccgctgcg ccgagctcgg ctgcacggta
     1741 cgcgccggcc gcggcgcgcc gctcgcgcgc ggcgctacgg tctggacggc catacgcccc
     1801 gagaagatca acatcgcgcg ccaggctccc gcggcgcagg agccggcacg ccccggcaac
     1861 ctggtgcgcg gcacggtcaa ggagatcgcc tacatgggcg acatgtcgat ctatctggtg
     1921 cgcatcggct cgggcaggct gctgcgcgtg acgctgccga acatcatgcg cggcgccgag
     1981 cggccgatcg cccgcgagga gacggtgtgg ctctcctggc acgactcgag cccggtagtg
     2041 ctcaccgagt agccgcggtg cgctggtctc gggcagcggg acgcggtctg gtcgcaggca
     2101 ttcccaccct gtggctgctg ctgctgttcc tggtgccctt catcatcgtg ttccgcatct
     2161 ccttctcgga ggtgcgcctg gcgataccgc cgtacacccc gctgatcgcc tggcaccacg
     2221 gcgagcccgc gctcgagctg cacggctcgg cctatgcgtt cctgttcacc gatccgctgt
     2281 atctcgcctc ctacctctac tcgctcaagg tggcggcggt ctcgacgctg tgctgcctgg
     2341 cgttaggcta tccgatggcc tacgccatcg cgcgcgcggg tcccgcgacc cgcccgctgc
     2401 tgctgatgct gatcgtgctg cccttctgga cctcgttcct gctgcgtgtc tacgcctgga
     2461 tcgggttgct gaacaacaac ggcgtcatca acaacgtgct gctgtacctg gggctgatcc
     2521 atcggccgct gcacctgctg cagaccgact tcgcggtgta catcggcatc gtctactcgt
     2581 acctgccgtt catgatcctg ccgctgtacg cgaatctcga gaagcacgac ctctcgctcc
     2641 tcgaggcggc cgcggatctc ggggcgcgcc cctggcgggc gttcctgcgg atcacgctgc
     2701 cgcagtccct gcccggcatc gcggcggggg cgctgctggt gttcattccg gcggtgggcg
     2761 agtacgtcat cccgacgctg ctcggccgca ccgaccagct gatgatcggg cgggtgctgt
     2821 cggacgagtt cttcgagaac cgtgactggc cggtggcgag cgcggtggcg atcctgatcc
     2881 tgctgctgct gatcgtgccg atcctgctgt tccagcgctt ccagcagcgc cagctcgagg
     2941 cgtcccggtg acctcgcgcg gcctgctgac acgcgcggca ctgctcctcg ggctggtgtt
     3001 cctgtacgtg ccgatcatct ccatgatcgt gttctccttc aacaattcgc gtctggtcag
     3061 cgtgtgggat gcggcgcatt cgcccacgct caagtggtac gcggcgctcg cccgcaacga
     3121 gcagatcctc ggcgccgcgt ggctgtcgat acgcatcgcg ctcatctccg ccagcgccgc
     3181 cgtgctgctc ggcacgctcg ccggggtggc gctggcgcgc ttcggcgcct accgcggccg
     3241 gctgctgctc gccggactga tcccggctcc gatcgtgatg ccggaagtca tcaccgggct
     3301 gtcgctgctg ctgctgttcg tggcgctcgg gcagctgatc ggctggccgc gcggcgtggg
     3361 cgcggttacc atcacgctcg cccacatcac cttctgcatg gcgtacgtga ccgtcgtggt
     3421 gcaatcgcgc ctcgccggct tcgacgagtc gctcgaggag gcggccatgg acctgggtgc
     3481 gcgcccgctg cgggtattcc tgcgcatc
//
LOCUS       DATDMP010000075         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1123462, whole genome shotgun sequence.
ACCESSION   DATDMP010000075 DATDMP010000000
VERSION     DATDMP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123462"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VI195_01280"
     CDS             complement(<1..699)
                     /locus_tag="VI195_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6823043.1"
                     /translation="MPGDCAIYLDSARPRGCADIKLNGGYRGKPMQKVAVRCCVAGGG
                     PAGVMAGLLLARAGIDVVVLEKHADFFRDFRGDTIHPSTLELMQELGSFDDLLKLPHE
                     KAHRIAFQFGALKGVVADFDRAKLPIPYIAFMPQWDFLDFLAHRAARYPTFSLKMSAE
                     VTGLIEEGGRVAGITATTPRGPLEVRADLVIAADGRHSLVREKSGLAVEELGAPMDVL
                     WFSLPRRDTDPDETM"
     gene            744..1184
                     /locus_tag="VI195_01285"
     CDS             744..1184
                     /locus_tag="VI195_01285"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY6823044.1"
                     /translation="MPIQITQTIRDLLNSALADGTPCLLGTASKDGHPNISPKGSIVV
                     FDDHSLCYWERSGRGAARRIAENAHVCVYYRNPAKAGKDFRGGALRFYGDVHVLPHGA
                     ERDKVWELIPEAERRPDPEKKGSAVLIKLHSVEELSGNVVMKHD"
     gene            1206..>2106
                     /locus_tag="VI195_01290"
     CDS             1206..>2106
                     /locus_tag="VI195_01290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY6823045.1"
                     /translation="MSAGAPEHGVSFVIPVFNKRRFLRQVIVGLAAQRGDFAREFIFI
                     DDGSTDGSRAELERLTPGLADSRILSQGNAGPSVTTNRGLREARFPLVKLVDGDDVLL
                     PAATAQLLTALAGHPDAALAFGQSATYADAEDALARIAQARPAEHPPERVEQALKPLL
                     RECYLGPSNCIIRRDLALSVGGCDERVFVQDYSLLLRLAERAPFVALDVPVVLHPATP
                     QDRLNDGGPQLLHDTNLTVVHFLAEHRVPPALATMATRRAMTRAWLWAKRREGVNPLS
                     RWYWLRLASYLPLPLLRARLLDAS"
BASE COUNT          356 a          729 c          670 g          351 t
ORIGIN      
        1 catcgtctca tcgggatcgg tgtcacgccg cggcaggctg aaccacagta catccatggg
       61 cgcgccgagc tcttcgacgg caagccccga tttctcgcgc accagcgagt ggcggccgtc
      121 ggccgcgatc acgaggtcgg cgcgcacctc gagcggaccg cgcggcgtgg tcgcggtgat
      181 gccggcgaca cggccaccct cctcgatgag ccccgtaacc tccgcgctca tcttcagcga
      241 gaaggtggga tagcgcgccg cgcggtgcgc caggaaatcg aggaaatccc attgcggcat
      301 gaaggcgata tagggaatgg gcagcttcgc gcggtcgaaa tcggcgacta cgcctttgag
      361 cgcgccgaac tggaaggcaa tgcgatgtgc cttctcatgc ggcagcttca agagatcgtc
      421 gaagctgccg agctcctgca tcagctcgag ggtcgaggga tggatcgtgt cgccgcggaa
      481 atcgcggaag aaatccgcgt gtttttccag gaccacgacg tcgatgccgg cgcgcgccag
      541 cagcagcccc gccataacac cggccggccc accgcctgcc acgcagcatc gtaccgccac
      601 tttttgcata ggctttcccc ggtaaccccc gttcaacttt atatcagcac agccgcgcgg
      661 tcttgccgag tcgaggtaaa tcgcgcaatc tccgggcatt cgcaaagaag accccggcgc
      721 cgccgggcaa gaggggagca agaatgccaa tccagatcac ccagaccatt cgcgatttgc
      781 tgaattcggc cctcgccgac ggcacgccct gcctgctcgg cacggcgagc aaggacgggc
      841 acccgaatat cagccccaag ggcagcatcg tcgtcttcga tgaccactcg ctttgctatt
      901 gggagcgcag cggccgcggc gcggcacggc gcattgccga gaacgcgcat gtctgcgtct
      961 attatcgcaa ccccgccaag gccgggaagg atttccgcgg cggcgcgttg cgcttctacg
     1021 gcgatgtcca tgtcctgccg cacggagcgg agcgcgacaa ggtctgggaa ctcattcccg
     1081 aagccgagcg ccggccggat cccgagaaga agggttcggc ggtgctgatc aaactccaca
     1141 gcgtcgagga gctatccggc aacgtggtga tgaagcacga ctgagcgggt tcagcgccgc
     1201 ttgccatgag cgctggagcg ccggaacacg gcgttagctt cgtcattccg gtattcaaca
     1261 agcggcgctt cctgcgccag gtcattgtcg gcctcgccgc gcagcgcggc gatttcgctc
     1321 gcgaattcat ctttatcgac gacggctcga ccgacggctc gcgcgccgag ctggagcggc
     1381 tgacgcccgg tctcgccgat tcccgcattt taagccaggg gaatgccggg ccgagcgtca
     1441 ccaccaatcg cggactgcgc gaagcgcgct tcccgctggt taagttggtc gacggcgatg
     1501 atgtgctgct gcccgctgcg acggcgcagc tcctcaccgc gctggcgggc catcccgacg
     1561 ccgccctcgc ctttggccaa tccgcgacct atgccgatgc cgaggatgcg ctggcccgca
     1621 tcgcccaggc gcggccggcc gagcacccgc ccgagcgcgt cgagcaagcg ctgaagccgc
     1681 tgctgcgcga gtgctatctc ggaccgagca actgcatcat ccggcgcgat ctcgcgctct
     1741 cggtgggcgg ctgcgacgag cgcgttttcg tgcaggatta ttcgctgctg ctgcgccttg
     1801 ccgagcgcgc gcctttcgtc gcgctcgatg tgccggtcgt gctgcacccg gcgacgccgc
     1861 aagaccggct caacgacggc gggccacaat tgctgcacga caccaacctg accgtcgttc
     1921 acttcctcgc cgagcaccgc gtcccgcccg cgctcgcgac catggccaca cggcgcgcca
     1981 tgacccgagc ctggctctgg gcgaagcgcc gcgagggcgt caacccgctc tcgcgctggt
     2041 attggctgcg gctcgccagc tatctgccct tgccgctgtt gcgcgcgcgt ttgctcgacg
     2101 ccagct
//
LOCUS       DATDMP010000076         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_679266, whole genome shotgun sequence.
ACCESSION   DATDMP010000076 DATDMP010000000
VERSION     DATDMP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679266"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VI195_01295"
     CDS             <1..332
                     /locus_tag="VI195_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823046.1"
                     /translation="ASTVHVGSSTSLTWAAYNVTSCNASGSWSGAQKTSGSLTITPTA
                     VGTDNYTLTCTNAHGSAQKTATLTVQAASSGGGGGGLDAATLIALLALGCTRALRPRV
                     RGESLRG"
     gene            453..1298
                     /locus_tag="VI195_01300"
     CDS             453..1298
                     /locus_tag="VI195_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823047.1"
                     /translation="MRALTAIGLCALALMRAASTCGATPVTYGTADTQYANFAFASEI
                     GSGVYEISGSVITVYTFEPYYTLRSAHPNGGRPGIRLIFPFTVGFFNFQTKDLVHLEL
                     PSSIGALSFEPGVELDYWINDVWDVYPYVKAGGTIASSSDANALIWGTGVRSDYRFEA
                     LESVGLWRTQLAYAGAHYQGNLPNESFTRLRNGVELRRVLDLSWHSRALQLAPYAIAD
                     VYFEAPSGPASGISPRTFQYEVGLMFGLSPRWEIHGVPLPRLGFGWREAGVLSGWRLV
                     LGDPF"
     gene            1384..1926
                     /locus_tag="VI195_01305"
     CDS             1384..1926
                     /locus_tag="VI195_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY6823048.1"
                     /translation="MKRVMCVAVAAWVAALSLAACAPYVRSDVNTALIGQVHCGSFAW
                     AGAFRSSSPLRSTIANPVNENRLRTAIASHLGGPIQPGPGNADCLVGYGIGGYYVMSG
                     VYPYPYGWAWSYPYGWGYPGPYAYPEGVIAIDLYDARTGQPLWHAWTEQSLFGVSGAE
                     AEKRIDSAVAAIFTKYPYTG"
     gene            2013..>2830
                     /locus_tag="VI195_01310"
     CDS             2013..>2830
                     /locus_tag="VI195_01310"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY6823049.1"
                     /translation="MRAPFGLSLIFLNVLLEQLGVPVPATPTLVVAGALAANGRLPVL
                     GVFALAVAASLIGDSSWYLAGRAYGVRVMKLLCRISLTPDTCVSQTQASFERWGARAL
                     LLAKFVPGLSMIAPPLAGATRMSFVRFAFFSAIGAALWVGAALIAGLLLKSQIARLLP
                     RLSGIGGTVVVLLLVLLAAYIGFKWWERRRFHALLDMARIGVDELNQRMQGDSLPLIV
                     DVRSSTSVGLERRRIPGALQVPVQEVERQSRSLPRDREIILYCNSPNEASAAQAA"
BASE COUNT          421 a          952 c          933 g          524 t
ORIGIN      
        1 cggcttcaac ggtgcatgtg ggcagctcca cctcgctcac ctgggccgcc tacaacgtca
       61 cgagctgcaa cgcctcggga agctggagcg gcgcacagaa gaccagcggc tcgctcacca
      121 tcactcctac ggctgtcggc accgacaact acactcttac ctgcaccaat gcccacggct
      181 ccgcgcagaa gaccgcgaca cttaccgtgc aggcggcctc ctcgggtggg ggaggcggag
      241 gcctcgatgc tgcaacgctc attgcgctcc tcgcgctcgg ctgcacgcgg gcattgaggc
      301 cgcgcgtgcg cggcgagtcg ctgcgcggct gagggctcgc cgcccgctgg atcgcgcacg
      361 ccgggccgtg cgggcgagcg ccggcgggat gttggaaccg gcaccggtct ggcgcgcgct
      421 ctttgctaaa ctccccgcac cggggaggca tcgtgagagc attgacggcc atagggttgt
      481 gcgctctcgc gctcatgcgt gccgcgagca cctgcggcgc gacgcctgtt acctacggca
      541 ccgccgacac ccagtacgcg aacttcgcgt tcgcgagtga gatcggctcg ggggtctacg
      601 agatctccgg cagcgtcatc acggtctaca ctttcgagcc ttactatacg ctgcgcagcg
      661 cgcaccctaa cggcggccgc cccggcatcc ggctcatttt cccgttcacg gtcgggttct
      721 tcaatttcca aaccaaggac ctggtgcatc tcgagctgcc gagctcgatc ggcgcactca
      781 gcttcgagcc gggcgtggag ctcgactact ggatcaacga tgtttgggac gtgtatccct
      841 acgtgaaagc gggtggcacc atcgcctcga gcagcgatgc gaacgccctc atctggggca
      901 caggggtgcg cagcgactat cgcttcgagg cgctcgagag cgtcggcctg tggcgcacac
      961 agctcgccta tgccggtgcc cattaccaag gcaacctgcc caatgaatcg ttcaccagat
     1021 tgcgcaacgg ggtggagctg cgccgcgtgc tcgatctgag ctggcactct cgtgccctcc
     1081 aactggcgcc gtatgcgatc gcggatgtgt acttcgaggc gccctccgga cctgcctccg
     1141 gcatcagtcc gcgcaccttc cagtacgagg ttggcctgat gttcggcctc tctccacgtt
     1201 gggagattca cggggtaccg ctgccgcgtt tgggattcgg ttggcgcgag gccggagtac
     1261 tgtcaggatg gcgactggtg ctcggtgatc ctttctgaag cgggtcaacg tggccccgcc
     1321 gcctgcgtta gcatgagcgt tggaatgctc gccccgttga gcccggtctg aggttggagc
     1381 tgcatgaagc gtgtcatgtg tgtcgcggtc gccgcctggg ttgcggcgtt gtcactcgcg
     1441 gcctgcgcgc cgtacgtacg ctcggatgta aacaccgcgc tcatcggtca ggtgcactgc
     1501 ggcagcttcg cctgggccgg cgcgttccgc agcagcagcc cgctgcgcag cacgatcgcc
     1561 aacccggtga atgagaaccg cctgcgcacg gccatcgcgt cccacctcgg cggacccatt
     1621 cagcccggcc cgggcaatgc ggattgcctg gtcggttatg gaatcggcgg ctattacgtg
     1681 atgagtggtg tctacccgta cccgtatggt tgggcctgga gctatccgta cggatggggc
     1741 taccccggtc cgtatgccta cccggagggt gtcattgcca tcgatctgta cgatgcgcgg
     1801 accggacagc cgctgtggca tgcctggacc gagcagagcc tcttcggagt gagcggcgcg
     1861 gaggccgaga aacgcattga ctcggccgtc gctgccatct tcaccaagta cccctacacg
     1921 ggctgacggg acgcagccta ccggcgctcg ggcgccggtg agcggccctc acctggctgt
     1981 tcgccgcagc ccgaacgcgg caagatccgc gcatgcgggc tccttttgga ctcagcctga
     2041 tcttcctcaa cgtactgctc gagcagctcg gcgtgcccgt gcccgcgact cccaccctgg
     2101 tcgtcgccgg cgcgctcgcg gcgaacgggc ggctgccggt actgggcgtg ttcgcgcttg
     2161 cggtagcggc gagcctgatc ggcgacagca gctggtacct ggcgggtcgc gcgtatggcg
     2221 tgcgcgtgat gaagctcctg tgccgcatct cactgacgcc cgacacgtgc gtcagccaga
     2281 cacaggcgag cttcgagcgc tggggtgcgc gcgcactcct gctcgccaaa ttcgtcccgg
     2341 gcctgtccat gattgccccg ccgctggccg gagccacgcg catgagcttc gtccgcttcg
     2401 ccttcttcag cgccatcggc gcggcgctgt gggtcggtgc ggcactcatt gcaggtctgc
     2461 tgttgaaatc gcagatcgcc cggctgttgc cgcggctcag cggcatcggc ggcacggtgg
     2521 tggtactgtt gctggtgctg ctcgccgcct atattggctt caagtggtgg gagcggcggc
     2581 gcttccacgc actgctcgac atggcgcgca tcggcgtcga tgagctcaac cagcgcatgc
     2641 agggcgatag cctgccgctg atcgtagatg tgcgttcatc cacctccgtc ggtctcgagc
     2701 gccgccggat tcccggtgcc ctgcaggtgc cggtgcagga ggtcgaacgg cagtcgcgca
     2761 gcctgccgcg cgatcgcgag atcatcctct actgtaacag cccgaacgaa gcctccgcgg
     2821 cgcaggcggc
//
LOCUS       DATDMP010000077         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1679309, whole genome shotgun sequence.
ACCESSION   DATDMP010000077 DATDMP010000000
VERSION     DATDMP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679309"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VI195_01315"
     CDS             <1..240
                     /locus_tag="VI195_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823050.1"
                     /translation="KVPAQALIDAAKAEKPPIVQEVRVFDLYQGSSLPGGKKSLAFRV
                     VMQDTERTLTDTEADAARDALIGLWGRRLGAILRA"
     gene            274..585
                     /locus_tag="VI195_01320"
     CDS             274..585
                     /locus_tag="VI195_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY6823051.1"
                     /translation="MTLTKAELADLLFEKVGLNKREAKDMVEAFFEEIRGALERGESV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPISARRVVTFHASQKLKAMVEKSRHGAQPEK
                     N"
     gene            560..964
                     /locus_tag="VI195_01325"
     CDS             560..964
                     /locus_tag="VI195_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY6823052.1"
                     /translation="MEPSRKRIDLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQ
                     LKPVKRRGNRRYYQHHEVLLIRRIRELLYEEGFTISGARNRLDNAATGDGHDGGGRAP
                     RAPSAASQSPLADASALKRELRDLLDSLRASH"
     gene            998..1074
                     /locus_tag="VI195_01330"
     tRNA            998..1074
                     /locus_tag="VI195_01330"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1032..1034,aa:Pro,seq:ggg)
     gene            1107..1844
                     /locus_tag="VI195_01335"
     CDS             1107..1844
                     /locus_tag="VI195_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6823053.1"
                     /translation="MRKVSSTANRTTVGDGIAAGNASWTFSDDVARTFSTHVSKSVPL
                     YNEGHDLICALSDFFVQDTSVCYELGASTGVLSGKLARRHAATKARFIGIESVPEMIA
                     QARRECGDLANLEFVQDDINLYPYETCDLVVSFCTVQFVPPYLRQKLFDRLYQSLNWG
                     GAMLIYEKVRGPDARFQDYMTAVYTDLKLERGYSPDEIVAKARSLKRVQEPFSTQGYL
                     DLFKRAGFTDVMTFIKWACFEGFFAIK"
     gene            complement(1857..>2376)
                     /locus_tag="VI195_01340"
     CDS             complement(1857..>2376)
                     /locus_tag="VI195_01340"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY6823054.1"
                     /translation="VRGGKNRAVYAKELVQGGMFSLCRNPLYVGNLLMAAGLALVINA
                     TAFYLIVIPAIVLAYRAIVAAEEAYLREKFGAQYEDYCARVNRWWPRLARFPAAVAGM
                     RFNWRRVLVKEYTTIFILFGAFFCLWEWSEYSERGAGALPSTHALLAGVIAWIASYLA
                     VRALKKSSLVRD"
BASE COUNT          448 a          767 c          783 g          378 t
ORIGIN      
        1 aaggttcctg cgcaagccct gatcgatgcg gccaaggctg aaaaaccccc catcgtccag
       61 gaggtccgcg tgttcgatct ctaccaggga agcagcttgc ctggcgggaa aaaaagcctt
      121 gcgttccggg tagttatgca agatactgaa cgaaccctca cggatacgga ggccgacgcg
      181 gcgcgcgacg cgctcatcgg gctgtggggg cggcgcttgg gcgccatcct gcgggcataa
      241 taatagaaat ccaagagata tcagggagtt gtaatgactt tgaccaaggc cgaactggcc
      301 gatcttttgt ttgagaaggt cgggctgaac aagcgcgagg ccaaggacat ggtggaggcg
      361 ttcttcgagg aaatccgggg agcgctggag cggggcgaga gcgtgaagct ctcgggcttc
      421 ggcaatttcc agctgcgcga caagccgcag cggccggggc gcaacccgaa gaccggcgag
      481 gagatcccga tcagcgcccg ccgcgtggtg acctttcacg ccagccagaa actgaaagcc
      541 atggtcgaga agtcgcgcca tggagcccag ccggaaaaga attgacctgc cgccgatccc
      601 tgcaaagcgc tacttcacaa tcggtgaggt aagcgagctc tgcggggtga agccgcacgt
      661 gctgcggtac tgggagcagg agttcacgca gctcaagccg gtcaagcgcc gcggcaaccg
      721 ccgctactac cagcaccacg aggtgctgct gatccggcgc atccgcgagc tgctctacga
      781 ggaaggcttc accatcagcg gcgcgcgcaa ccgactggac aatgccgcca ccggcgacgg
      841 ccacgacggc ggcggccgtg cgccgcgcgc gccttcggcg gcctcgcagt cgccgcttgc
      901 cgacgcctcc gcgctcaagc gcgagctgcg cgacctgctc gacagcctgc gcgcctcgca
      961 ctagccgcca ccccgcgtag ggtaaaatcc gcagtctcgg ggcgtagcgc agcctggtag
     1021 cgtacctgca tggggtgcag gtggtcggag gttcaaatcc tctcgccccg accagaacct
     1081 cgcaacgttc cactcgatcg gtgcttatgc gcaaggtctc gagtacggcg aacaggacca
     1141 cggtgggcga cggcatcgcc gccggcaacg ccagctggac gttcagcgac gacgtggcgc
     1201 gcaccttttc cacccacgtc agcaagtcgg tgccgctgta caacgagggg cacgacctca
     1261 tctgcgcgct gtcggacttc ttcgtgcagg acacgtccgt ctgctacgag ctgggcgcgt
     1321 ccaccggggt gctgagcggc aagctggcgc ggcgccatgc cgcgaccaag gcgcgtttca
     1381 tcggcatcga atcggtgccg gagatgatcg cgcaggcgcg ccgggagtgc ggcgacctgg
     1441 cgaacctcga gttcgtccag gacgacatca acctgtaccc gtacgagacc tgcgatctcg
     1501 tcgtgtcgtt ctgcacggtc cagttcgtgc cgccctacct gcggcagaag ctgttcgatc
     1561 gcctctacca gagcctgaac tggggcgggg cgatgctgat ctacgagaag gtgcgcggcc
     1621 cggatgcgcg cttccaggac tacatgacgg cggtgtacac cgacctgaag ctggagcgcg
     1681 gctattctcc cgacgagatc gtcgccaagg cgcgcagcct caagcgcgtg caggagccgt
     1741 tctcgacgca gggctatctc gacctcttca agcgcgccgg cttcacggac gtgatgacct
     1801 tcatcaagtg ggcctgtttc gagggcttct tcgccatcaa gtagcccggg cgcgcgtcag
     1861 tccctgacga ggctcgattt cttcagcgcg cgcaccgcca gatacgaggc gatccacgcg
     1921 atcacgcctg ccagcagcgc gtgggtcgac ggcagcgcgc ccgcgccccg ttcgctgtat
     1981 tcgctccact cccacaggca gaagaacgcg ccgaagagga tgaagatcgt cgtgtactcc
     2041 ttgaccagga cgcgccgcca gttgaagcgc atgccggcga ccgcagccgg gaagcgggcg
     2101 agccgcggcc accagcggtt gacgcgcgcg cagtagtcct cgtattgggc gccgaatttc
     2161 tcgcgcaggt aggcttcctc cgccgcgacg atggcgcggt aggcgagcac gatggccggg
     2221 atgacgatca ggtagaacgc cgtggcgttg atcaccagcg cgagcccggc cgccatcagg
     2281 aggttgccga catagagcgg attccggcag agggagaaca tgccgccctg cacgagctct
     2341 ttggcgtaga ccgcccgatt cttgccgccg cgaacg
//
LOCUS       DATDMP010000078         2429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_346376, whole genome shotgun sequence.
ACCESSION   DATDMP010000078 DATDMP010000000
VERSION     DATDMP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346376"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2429
                     /locus_tag="VI195_01345"
     CDS             <1..>2429
                     /locus_tag="VI195_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6823055.1"
                     /translation="TFRLKYAIASFLVVLLAAAAVAVLFMLRHSADARSLSALAEHAA
                     RERLDPELEARARGLAAHAADAIAGAVRAEDAAGIVRRLQPFTDDPTVEAITVSSRND
                     QTLFHWQRDAAAPAGTLTAQAESKVTGYAENIPGAVTPETLATLTVVLEQAAPVPGVG
                     LGARLEEATAQRTRSTWWLALAVAAGGALLAAALMWRAVSHLQRPLYSLIRSAERIGQ
                     GDYTRPVEVQRRDVLGELQQALERMRGHLRQSTVNKSYLHSVLNSMTDAVFVTSPDGV
                     IKMANAAACKLLAFGEEELLGRSILALLEERERGDFDLQQAAHETRETLVRTRSGQTI
                     PVSITGSRIDSDDPQFQGNIFVARNITDRKRAERRIRYLARYDALTKIPNRMQFHHVL
                     QQTIARALRAGQVVAVLYLDMDRFKEVNDTFGHGAGDRVLEVLAERLTRAVPRETVVG
                     RLAGDEFALFVEALPADADNRGPIAQLARALLTEVSRPFQLNQHEVFLTASVGIAFCP
                     RDAENVIDLIRNADAAMYYSKQNGGNTFAFYSPEMNAAAVERLMLKSKLRRALERDEL
                     EIRYQPKVDLRTGRLSGAEALLRWRLPGHGDIPPSQFIPLAEETNLILDIGEWVLNRV
                     CLDYRRMQGQGGDPGRISLNLSLKQLRQASFILRCRSVFRRHHVSPTAFELEITETTL
                     MADPQRTVRLLDELYAMGLHLSIDDFGTGYSSLSALQQFPIGTLKIDQSFVRDATEDA
                     GDATIVRTIIEMGRSLGMEVIAEGVESQAQLQFLRVNGCHYGQGRLFGEPCSVEELLA
                     LLATQAAGDAG"
BASE COUNT          383 a          868 c          804 g          374 t
ORIGIN      
        1 cactttcagg ctgaaatacg ccattgcgag cttcctcgtc gtgctgctag ccgcggccgc
       61 ggtggcggtg ctgttcatgc tgcggcacag cgccgatgcg cgctccctca gcgcgctcgc
      121 cgagcacgcc gcccgcgagc gcctcgaccc ggagctcgag gcgcgggcgc ggggccttgc
      181 ggcgcacgcc gcggatgcca tcgccggcgc agtgcgcgcc gaagatgccg cgggcatcgt
      241 gcgccggttg cagcccttca ccgacgatcc caccgtagaa gccatcacgg tgagctcccg
      301 caacgaccag acgctgttcc actggcagcg cgatgcggcg gcaccggccg gcaccctcac
      361 cgcacaggca gagtcgaagg tcaccggcta cgcggagaac atccccggcg cggtcacgcc
      421 cgagacgctc gccacgctca ccgttgtgct cgagcaggcc gccccggtcc ccggcgtcgg
      481 cctcggggcg cgtctggagg aggcgaccgc gcagcgcacg cgctcgacgt ggtggctggc
      541 gctcgccgtg gccgcaggcg gggcgctgct cgccgccgcg ctcatgtggc gcgccgtgag
      601 ccacctgcag cgcccgctct attcgctcat ccgcagcgcc gagcgcatcg gccagggcga
      661 ttacacgcgc ccggtcgagg tacagcggcg cgatgtgctc ggggagctgc agcaggcact
      721 cgagcgcatg cgcggccacc tgcgccagtc gaccgtcaac aagagctacc tgcacagtgt
      781 gctcaacagc atgaccgatg cggtgttcgt cacctccccc gacggggtga tcaagatggc
      841 caacgccgcc gcctgcaagc tgctcgcctt cggcgaggag gagctgctcg ggcgcagcat
      901 cctcgcgctg ctggaggagc gcgagcgcgg agacttcgat ctgcagcagg ccgcgcacga
      961 gacgcgcgag acgctggtgc gcacccgctc cggccagacg attccggtat ccatcaccgg
     1021 ctcgcgcatc gatagcgacg accctcagtt ccagggcaac atcttcgtcg cgcgcaacat
     1081 caccgaccgc aagcgcgccg agcgccgcat ccgctatctg gcgcgctatg acgccctcac
     1141 caagattccc aaccgcatgc agttccacca cgtgttgcag cagaccatcg cgcgtgcgct
     1201 gcgcgccggt caggtggtgg cggtgctgta cctcgacatg gaccgcttca aggaggtgaa
     1261 cgacaccttc ggccacggag ccggggaccg cgtgctcgag gtgctggcgg agcgtctgac
     1321 gcgcgccgtg ccgcgcgaga ccgtggtcgg ccgcctggcg ggcgatgagt tcgcgctctt
     1381 cgtggaagcg ctgcccgccg atgcggacaa ccgcgggccc atcgcccagc tcgcgcgcgc
     1441 gctgctcacc gaggtgagcc gccccttcca gctcaatcag cacgaggtgt ttctcaccgc
     1501 gagcgtcggt attgccttct gcccgcgcga tgccgagaac gtgatcgacc tgatccgcaa
     1561 cgccgacgcc gccatgtatt actccaagca gaacggcggc aacaccttcg ctttctattc
     1621 accggagatg aatgccgcgg cggtcgagcg gctgatgctc aagagcaagc tgcgccgcgc
     1681 gctcgagcgc gacgagctcg agatccgcta tcagcccaag gtggacctgc gcaccggacg
     1741 cctgagtggc gccgaggcgc tgctgcgctg gcggctgccg gggcacggcg acatccctcc
     1801 ctcgcagttc attccgctcg cggaggagac caacctcatc ctcgacatcg gcgaatgggt
     1861 gctgaaccgc gtctgcctcg actatcgccg catgcagggt cagggagggg atccggggcg
     1921 catatcgctc aatctctcgc tcaagcagct gcgccaggca agcttcatcc tgcgctgccg
     1981 ttcggtgttc cgtcgccacc acgtctcgcc caccgctttc gagctggaga tcaccgagac
     2041 caccctcatg gccgacccgc agcgcaccgt gcggctgctc gatgagctgt atgccatggg
     2101 cctgcacctg tcgatcgatg acttcggtac cggctattcc tcactctcgg cgctgcagca
     2161 gtttccgatc ggcacgctga agatcgacca gtccttcgtg cgcgatgcga ccgaggatgc
     2221 cggggatgcc accatcgtgc gcaccatcat cgagatgggt cgcagcctcg gcatggaggt
     2281 gatcgccgaa ggagtggagt cgcaggcaca gctgcagttc ctgcgcgtca acggctgcca
     2341 ttacggccag gggcggctgt tcggcgagcc ctgcagcgtg gaagagctgt tggcgctgct
     2401 cgcgacccag gcggccgggg acgcggggc
//
LOCUS       DATDMP010000079         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_679518, whole genome shotgun sequence.
ACCESSION   DATDMP010000079 DATDMP010000000
VERSION     DATDMP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679518"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1318)
                     /locus_tag="VI195_01350"
     CDS             complement(50..1318)
                     /locus_tag="VI195_01350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY6823056.1"
                     /translation="MAAERLAEIRNYTMNFGPQHPAAHGVLRLVLEMDGEVIHKADPH
                     IGLLHRATEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVLAIERLLGIEPPERARYI
                     RVMFDEITRILNHLMFLGSHSLDIGAMTVFLLAFKEREELMDVYEAVSGTRMHATYYR
                     PGGVYRDLPESMPKYQASKWHSARAVARLNEARSGTMLDFIQVFTERFPAAIDDYEAL
                     LTDNRIWKQRTVNIGVVSPERALQLGFSGPMLRGSGVAWDLRRKQPYEVYPRMEFDIP
                     VGVNGDCYDRYLVRIEEMRQSNRIIRQCVEWLRSHPGPVMIDDTKIRAPSREHMKEGM
                     ESLIHHFKFFTEGYEVPEGETYAAVEAPKGEFGIYLVSDGANKPYRLKCRAPGFAHLA
                     AMEEMVRGHMLADVVAVIGSQDIVFGEIDR"
     gene            complement(1318..>2078)
                     /locus_tag="VI195_01355"
     CDS             complement(1318..>2078)
                     /locus_tag="VI195_01355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY6823057.1"
                     /translation="SCRRRSPAPARSSGDGNGADGRLSELIEALAAKVDAQLQGGVRR
                     LPALAHELAYEVEAGELLAVCHTLRDAAELRFAMLMDLAGIDFLHYGRDEWQTSSATG
                     SGFSRGRVARDAPDPLEPGRFAVVYQLLSIAHNRRLRLRVKCPDSAAPGVDSVVEVWA
                     GANWYEREAFDLFGIVFRGHPDLRRILTDYGFIGHPFRKDFPLIGNVEVQYDPERRRV
                     VYRPVSITPRVLVPKVIRHDHRYEPALRDTQVPR"
BASE COUNT          317 a          699 c          710 g          352 t
ORIGIN      
        1 ttgctcacga gcgcggtgcc gccgacaccg atgctgctgc gctcgccgct caccggtcga
       61 tctccccgaa cacgatgtcc tgcgagccga tcaccgcgac cacgtcggcg agcatgtggc
      121 cgcggaccat ctcctccatc gccgcgaggt gcgcgaagcc cggagcgcgg cacttcaggc
      181 ggtacggctt gttggcgccg tcggacacca ggtagatgcc gaactcgccc ttgggcgcct
      241 cgaccgcggc gtaggtctcg ccctccggga cttcgtagcc ctcggtgaag aacttgaagt
      301 gatggatgag cgactccatg ccctctttca tgtgctcgcg cgagggtgca cggatcttgg
      361 tgtcgtcgat catcaccggg cccgggtgcg agcgcagcca ctccacgcac tggcggatga
      421 tgcggttcga ctggcgcatc tcctcgatgc gcaccaggta gcggtcgtag cagtcgccgt
      481 tcacgccgac cggaatgtcg aactccatgc gcggatagac ctcgtagggc tgcttgcggc
      541 gcagatccca ggccacgccc gagccgcgca gcatcggccc ggagaagccg agctgcagcg
      601 cgcgttccgg cgacaccact cctatgttga cggtgcgctg cttccagatg cggttgtcgg
      661 tgagcagcgc ctcgtagtcg tcgatcgccg cgggaaagcg ctcggtgaag acctggatga
      721 agtccagcat cgtcccggag cgcgcctcgt tcagccgtgc caccgccctc gccgagtgcc
      781 acttgctcgc ctggtacttc ggcatcgact ccggcaggtc gcggtacacc ccgcccgggc
      841 ggtagtaggt cgcgtgcatg cgcgtgccgg ataccgcctc gtagacgtcc atgagctcct
      901 cgcgctcctt gaaggcgagc aggaacaccg tcatcgcgcc gatgtcgagg ctgtgggagc
      961 cgaggaacat caggtgattc aggatgcgcg tgatctcgtc gaacatgacg cggatgtaac
     1021 gggcgcgctc cggcggctcg attccgagca gccgttcgat ggcgagcacg taggcatgct
     1081 cgttgcacat catcgagacg tagtcgagcc ggtccatgta gccgatcgac tgattgaagg
     1141 gcttggactc ggcgagcttc tcggtggcgc ggtgcaggag cccgatgtgc gggtcggcct
     1201 tgtggatcac ctcgccgtcc atttccagca ccagccgcag cacgccgtgg gcggcggggt
     1261 gctgcggccc gaagttcatg gtgtagttgc ggatctccgc gaggcgttcg gcggccatca
     1321 gcgcggcacc tgggtgtcgc ggagcgcggg ctcgtagcgg tggtcgtggc ggatcacctt
     1381 cggcaccagc acccggggcg tgatgctcac cggccggtac acgacccggc gccgctccgg
     1441 gtcgtactgc acctcgacgt tgccgatgag ggggaagtcc ttgcggaacg ggtggccgat
     1501 gaagccgtag tcggtgagga tgcgccgcag gtccggatgg ccccggaaca cgatgccgaa
     1561 caggtcgaag gcctcgcgct cgtaccagtt ggcgcccgcc cacacctcca ccaccgagtc
     1621 gacccctggg gcggcgctgt ccgggcactt cacccgcagc cgcaggcggc ggttgtgcgc
     1681 gatcgacagc agctggtaga ccaccgcgaa gcggccgggc tcgagcggat ccggggcgtc
     1741 gcgcgccacg cggccgcggc tgaatccgct gccggtggcg ctcgaggtct gccactcgtc
     1801 gcggccgtaa tgcaggaagt cgatgccggc cagatccatc agcatcgcga atctgagctc
     1861 ggccgcatcc cgcagcgtgt ggcacacggc gagcagctcg ccggcctcga cctcgtaggc
     1921 gagctcgtgc gcgagcgcag gcagccgccg cacgccgcct tgcagctgcg catcgacctt
     1981 ggcagcgagt gcctcgatga gctcgctcaa gcgtccatcc gctccattgc cgtcacctga
     2041 ggatcgtgct ggtgcgggcg atcttcttct gcagctgc
//
LOCUS       DATDMP010000080         2686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_235545, whole genome shotgun sequence.
ACCESSION   DATDMP010000080 DATDMP010000000
VERSION     DATDMP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235545"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VI195_01360"
     CDS             <1..593
                     /locus_tag="VI195_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY6823058.1"
                     /translation="HANDLDTAAFAELAALARHPEVVAVGECGLDYFRNLSPRAAQLQ
                     AFHRQLELAIEIGKPVFLHQRDAHDDFLATLREHAGAWRGVAHCFTGSAEQLHAYLQL
                     GLAIGITGWICDERRGAHLLALVPQIPAGRLLLETDAPYLLPRDLTPKPASRRNEPAY
                     LPHIAATVARARGEPLASLARSSTESARLLFGLPPA"
     gene            670..2091
                     /locus_tag="VI195_01365"
     CDS             670..2091
                     /locus_tag="VI195_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6823059.1"
                     /translation="MDLDRLEARIARTWDDSILERLIAYVRIPNKSPLFDPQWERNGY
                     MEAAVQLMAEWCRTQNVRGARIEVRRLPGRTPLLFVDVPGDIPGCVLLYGHLDKQPEF
                     TGWLPGLGPWEPVVREGRLYGRGAADDGYAVFSSLTAIAALKEQQVRLPRCVLLIEAC
                     EESGSTDLPAHLAALGDAFGEPSLVVCLDAEAGNYEQVWCTTSLRGNLVGTLRVRVLK
                     EGVHSGMATGIAPTPFRILEQLLARIENPVTGDMLLDELHVSLPRDRRAQASTAAQVL
                     GGDMAGKLPWLSGVQPVSNDPAELIINSTWRATLAVTGAEGLPPVGSAGNVLLPELAL
                     KLSLRLPPTCDAERAEHAVRQALERDPPYRAQVSFEDAAGNGGWNAPEFAPWLEESIA
                     RASQAVFGKPAVHIGCGGTIPFMGMLGERFPRTQFFITGVLGPQANAHGPNEFLHIDY
                     TKKLTACVSLVLADHARVLSAPT"
     gene            complement(2171..>2686)
                     /locus_tag="VI195_01370"
     CDS             complement(2171..>2686)
                     /locus_tag="VI195_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEY6823060.1"
                     /translation="WTWYSRYRARKALYAAVTSGAADHLSDSLVPDGMGGSFHVDFLL
                     LTLRGVVVIDLRNVKGNIFGGDQMAEWTVMDGARRFTFTNPQSALYDRIAAVKAIAGD
                     VPVEGRIVFTRRGRFPKGLPKWTLMLDALRAEFPAADFESPAETVARFREGWQSLRAA
                     VKPSSMGQPGR"
BASE COUNT          436 a          914 c          870 g          466 t
ORIGIN      
        1 accacgcaaa tgatctcgac accgcggctt tcgctgagct tgccgctctc gcccggcacc
       61 ccgaggtggt tgcggtgggc gaatgtggtc tcgactattt ccgcaatctt tcaccgcgcg
      121 ccgcgcaact gcaggccttc caccgccagc tcgagcttgc catcgagatc ggcaagccgg
      181 tatttctgca ccagcgcgac gcccatgacg atttcctcgc caccctgcgc gagcacgccg
      241 gcgcgtggcg gggcgtcgcg cattgtttca ccggatcggc cgagcagctg cacgcatatc
      301 tgcagctcgg gctggcgatc ggcatcaccg gctggatatg cgatgagcgc cgcggcgcgc
      361 atctgctcgc cctcgtgccg cagataccgg cggggcgatt gctgctcgag accgatgctc
      421 cttatctgct gccgcgcgat ctcacgccga aacccgcgtc gcgccgcaac gaaccggcgt
      481 atcttccgca tatcgccgcc acggtggcgc gcgcacgcgg tgagccactc gcttccctcg
      541 cccgctcgag caccgagtcc gcacgtctat tgttcgggct gccgccagcg taagtcggca
      601 gcagtgtcct gagatataac ctacagaaac aattgtaacc tgctgttatt aaacgaatga
      661 ggccgaacca tggatcttga tcggctcgag gcccgcatcg ccaggacgtg ggatgactct
      721 atcctcgaac ggctgattgc ctatgtacgc attccgaaca agtcgccgtt gttcgatcct
      781 caatgggagc gcaacggcta catggaggcg gccgtgcaac tcatggcgga gtggtgtcgt
      841 acacagaatg tccgcggcgc acgcatcgag gtgcggcgcc tcccggggcg taccccgctg
      901 cttttcgtcg acgtgccggg ggacatcccc gggtgcgtcc tgctctacgg ccatctcgac
      961 aaacagccgg agttcaccgg ttggcttccg gggctcggtc cgtgggaacc agtggtgcgc
     1021 gagggtcggc tctacggtcg cggtgcggcc gacgatggct atgcggtgtt cagctccctt
     1081 accgccattg cggcgctgaa ggagcagcag gtgcggctgc cgcgctgcgt gctgctcatc
     1141 gaggcctgcg aagaaagcgg cagcaccgat ctgccggcgc acctcgccgc actcggcgac
     1201 gccttcggcg agccctcgct cgtggtgtgc ctcgatgcgg aggccggcaa ctacgagcag
     1261 gtgtggtgca cgacctcact gcgcggaaat ctcgtcggga cactgcgcgt gcgtgtgctc
     1321 aaggagggcg tgcactcggg catggccact gggatcgccc cgacaccgtt tcgcatcctc
     1381 gagcagctgc tcgcgcgcat cgagaatccc gtcaccgggg acatgctgct cgatgagctg
     1441 catgtcagcc tgccccgtga ccggcgcgcg caggcgagca cggctgcgca ggtgctcggc
     1501 ggcgacatgg ccgggaagct gccgtggctg tcaggtgtcc agccggtctc caacgatccc
     1561 gccgagctca tcatcaacag tacctggcgc gccacgctcg ccgtgaccgg ggccgaagga
     1621 ctgccgccgg ttggctcggc cggcaacgtg ctgttgccgg agctcgcgct caagctgtcc
     1681 ctgcgcctgc cgccgacctg cgacgcggag cgcgccgagc atgccgtgcg ccaggctctc
     1741 gagcgcgatc cgccctaccg ggcgcaggtg agcttcgagg acgccgccgg caacggcggc
     1801 tggaacgccc cggagttcgc cccgtggctc gaggaatcca tcgcacgcgc ctcgcaagcg
     1861 gtgttcggca agccggccgt gcacatcggt tgcggtggga ccattccctt catgggcatg
     1921 ctgggcgagc gcttcccgcg cacgcagttc ttcatcactg gcgtgctggg cccgcaggcg
     1981 aatgcgcacg gccccaacga gttcctgcac atcgactaca cgaagaagct gacggcgtgc
     2041 gtgtcgctgg tactcgcgga tcacgcgcgc gtcttgtctg cgccgacctg acgaaactgc
     2101 ctgcgcgaat agttaaccgt tcggtgaact attgccgaag gatgtgttgt ctgtgcctcc
     2161 ttgcggaggg ctatcggccg ggctggccca tggagctcgg cttcaccgca gcgcgcagac
     2221 tctgccagcc ctcgcgaaag cgcgcgacgg tctccgccgg cgactcgaag tccgccgcgg
     2281 ggaactctgc gcgcagcgca tcgagcatga gtgtccactt gggcagaccc ttggggaagc
     2341 ggccgcggcg cgtgaagacg atgcgaccct ccaccggcac atcgccggcg atcgccttga
     2401 ccgcagcgat ccggtcgtag agcgcgctct gcggattggt gaaggtaaag cggcgcgcgc
     2461 catccatcac cgtccactcg gccatctgat cgccgccgaa aatgttgccc ttcacattgc
     2521 gcagatcgat caccaccacc ccacgcagcg tgagcagcag gaaatccacg tggaacgagc
     2581 cgcccatgcc atcgggcacg agtgaatcgc tgaggtgatc tgccgcaccg ctggtgacgg
     2641 ctgcgtacag cgccttacgg gcacggtagc ggctgtacca ggtcca
//
LOCUS       DATDMP010000081         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_235547, whole genome shotgun sequence.
ACCESSION   DATDMP010000081 DATDMP010000000
VERSION     DATDMP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235547"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1576
                     /locus_tag="VI195_01375"
     CDS             119..1576
                     /locus_tag="VI195_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823061.1"
                     /translation="MKTIAIRPLLGLVLLAGFAGAPATAGADASANGKVTIGVVLPQG
                     QLGNGADAAEPLRQTVMNRLRAPGVEVVALAGSTPDEIDADAKGKGCSQVLYTHVEQK
                     HGAGGLFSKLAPLAGVLPVMAAAGGGGGGGGGSGLTGLLTQTAANAATSAAASAAQKQ
                     MLGTQQQILAEQQPALALQNAARASIKRGDSISFDYRLVAVGSAAPIRTDTLHTKADA
                     DGQDVLTPLVEQLGNAVSADSQGAGNGSSGGGGGGATGSATAPSAGQAGHTGFMHGLF
                     GAREKGASSSASTSAPTGGGMPDCAQIASMPNAPVSLEACQKMAGAQQAYDRALADPS
                     ASRPGDEQMSCGQILAELRQQQISAPDKAKVAATTAAATKEQALLKKHMAEDSAIMAE
                     EQAKVDAAVAVDQATGYATAGLVHPNAASAAAEAGQARINVVGKRQAEERRPTEQKLL
                     GGVADLGMDAAQQLASNPRLGRLMQLAQSHGCKGI"
     gene            1638..2291
                     /locus_tag="VI195_01380"
     CDS             1638..2291
                     /locus_tag="VI195_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823062.1"
                     /translation="MVRGAWKRAGLLGAAATLLTLHGAVRGAAPAPGPPLAPVAPAAI
                     PPAVGAPSTDGSFPLTLTLTGSLAGIHPDVAKAAWLCSARILTQPAIDAELRKLQSLS
                     GQAVRTEFSSFLEYRAHYLGQQVTVEVPLASGTTGAVPPISLTMKRDDLIDPATRRAI
                     DQPAVLIGCWLELTNAAGQGGFATQITGPVSGGAPLTTMLQVRTPPWLLASASIANE"
BASE COUNT          408 a          840 c          869 g          327 t
ORIGIN      
        1 ggcgccggcg tgacagggca ggcacccgtg cgcgagccga cttcgtgagc cgctccggcc
       61 ccgttctgcg cgttaatagg tgagagccac gggttcaccc attcgccgag gaacggtcat
      121 gaaaaccata gctatacgac cgctgttggg gttggtgctt cttgcgggct ttgcgggtgc
      181 tcccgccacg gcgggcgcgg acgcgagtgc caatggcaag gtaaccattg gcgtagtgct
      241 tccccagggg cagctcggca atggggcgga tgcggccgag ccgctgcgcc agaccgtcat
      301 gaaccgcctg cgagcaccgg gtgtcgaggt ggtggctctc gcggggagca ccccggacga
      361 gatcgacgcc gacgcgaagg ggaagggctg cagccaggtg ctctacacgc atgtggagca
      421 gaaacacggt gccggcgggc tcttcagcaa gctcgcgccc ctcgccggcg tactgcccgt
      481 catggccgcc gccggcggtg gtggtggtgg cggcggcggc agcggactga ccggcctgct
      541 gacccagacc gccgcaaacg cggcgaccag cgcggcggcc tcggcggcac agaagcagat
      601 gctgggaacg cagcagcaga tactcgctga acagcagcca gcgctcgctc tgcagaacgc
      661 cgcccgcgcc agcatcaagc gcggtgacag catttcattc gactaccgcc tggtcgcggt
      721 ggggagcgcc gcgccgatca ggaccgatac cctgcacacc aaagcggacg ccgatggtca
      781 ggacgtgctg accccgctgg tcgagcagct tggcaacgcc gtgagcgccg attcccaggg
      841 cgccggaaac ggcagcagcg gcggcggcgg cggtggcgcg accgggagtg cgactgcgcc
      901 cagcgccggt caggccgggc acaccgggtt catgcacggc ctcttcggcg cgcgtgagaa
      961 aggcgcgagc agctcggcat cgacctccgc cccgacgggc ggtgggatgc ccgactgcgc
     1021 gcagatcgcc agcatgccga acgccccggt atcgctcgag gcgtgccaga agatggcggg
     1081 tgcacagcaa gcctacgacc gggcactcgc cgacccgagc gcctcccgtc ccggcgacga
     1141 gcagatgagc tgcggccaga tcctggccga gctgcggcag cagcagatca gcgcccccga
     1201 caaggccaag gtagccgcga cgaccgcagc cgcgaccaag gagcaggccc tgctcaagaa
     1261 gcacatggcg gaagatagcg cgatcatggc ggaggagcag gcgaaggtgg acgcggcggt
     1321 cgcggtcgat caggcgaccg ggtacgcgac cgccggcctc gtgcatccga atgcggcgtc
     1381 ggctgccgct gaagcgggtc aggctcgcat caacgtggtg ggtaagcgcc aggccgagga
     1441 gcgcagaccc acggagcaaa agctgctcgg cggagtggcc gacctcggga tggatgccgc
     1501 acagcagctc gccagcaatc cgcgcctcgg gcggctgatg caactcgctc agtcgcacgg
     1561 ctgcaagggg atctgagcct aaggcgtcgc agcctgcgtc gagccggatg cgggggtcag
     1621 ggcacaggga tctggtcatg gtgcgaggtg cttggaagcg ggccggcctg ctgggcgccg
     1681 ccgccacgct gctgaccctt cacggcgcgg tgcgcggggc agcgcccgcg cctggcccgc
     1741 ctctcgcgcc ggtcgcgccg gcggccatcc cgccggcggt gggcgccccg agcactgacg
     1801 gcagcttccc gctcacactc acgctcaccg ggtcccttgc gggcattcat ccggatgtcg
     1861 cgaaggccgc ctggctctgc tccgcccgga tcctcacgca gcccgccatc gacgccgagc
     1921 tcaggaagct tcaaagcctg agcggccagg cggtgcggac cgaattcagc tcctttctcg
     1981 aataccgggc gcactacctc ggccagcagg tgacggtgga agtcccgctc gcatcaggga
     2041 cgaccggcgc cgtaccgccg atcagcctca ccatgaagcg cgatgacctg atcgatcccg
     2101 ccacccgccg cgcgatcgat cagccggccg tcctgatcgg atgctggctc gagctcacca
     2161 acgctgcggg gcaaggaggc tttgcgacgc agattacagg accagtgagc ggtggcgccc
     2221 ccctcacgac catgctgcag gtcaggacac cgccgtggct cctcgccagt gcgagcatcg
     2281 caaatgagtg agtcatcgat ccggcttgac cctgggtcgc atcgctgcgc cgtagcgatg
     2341 ggcagggccg cggcagtcgc ctgctgcgtc atggtcctgc tggcgcgcag tcctcccatc
     2401 catgcagccg cgatgactgc gcctgcggcg cccgttccgg tgag
//
LOCUS       DATDMP010000082         2798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1568836, whole genome shotgun sequence.
ACCESSION   DATDMP010000082 DATDMP010000000
VERSION     DATDMP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568836"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..>2798)
                     /locus_tag="VI195_01385"
     CDS             complement(107..>2798)
                     /locus_tag="VI195_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY6823063.1"
                     /translation="ARVATGAAAAAHAPTDPRVSKPATSMLPRLESSLIARVVGRSVA
                     HSWMLLAVVIILCSAMAQYVRGHFAMTTDTRALLSPTLPWRVRQADFNAAFPLGNPDI
                     VVVVDGRTPELSAMGASALAASLVADTRLFRSVQQPDAGPFWARNRLLFASTEDVKTV
                     CAQIVKVQPFLGSMASDPSLRGLANTLSLIAKGVNNGESSVQELRTPIRALTDALAGL
                     TAGRQPFFSWRTLITGNEADERELRHIVLVDPRLDFTRLQPGKLPIDAIRSTAQRLQL
                     DPAHGLSVRLTGPVPLQDEEFATLTQRAGLIAVLASVAIVLMLWFAVRSARLIAAILI
                     TTLVGLLTATALGLALFQRFNVISVAFIPLFVGMGMDLGIQFSVRYRTERAAGGALGS
                     ALIATGRSMGKSLTLAAAAIGAGFLAFTPTAYYGVSQLGVIAGVGMFTALGLNLTLLP
                     ALIALIRAPGVPLRAGAQLAVIDDYVLGHRVLVVGSGAAAALICAALLPLVRFDFNPI
                     HLRAATVESVATLVDLLRDPERSPNTLEVLRPSLAAADELAAVFRGNPTVHSAHTLSS
                     FIPGDQAHKIALIADTANLVDLTLNPLEVAAPPTDAELIDSLQRAAGKLRQISTQDQA
                     LRTDTQRLAVALEALARGTPAARGGAAHMLIPSFMTTLAQIRSFLQPQPVSLATLPEE
                     TVRAWQTADGRARVSVLPRGDSNDDAVLRRFAAAGLKIAPDATGPAIYLQEYARAVVD
                     AFIEAGVLSFVAICGLLLIALRRVRDVAITMAPIVLTGLLTMGTCVLIGQPLNFANII
                     ALPLLFGIGVAFHIYFVMGWRSGGSHLLTSSLARGVFFSALATATGFGSLWASSHPGT
                     ASMGKLLMISLVWTLASALLFQPALMGPAPPGSQAVRVTS"
BASE COUNT          537 a          892 c          967 g          402 t
ORIGIN      
        1 aacggtgtga gcgagctcgc tctgcgccgc gcggacttcg ccgcggcgct agccgccggt
       61 ggagcgcccg tgctgattgc acatctgaac aaggccagcg acggcctcat gaagtaacac
      121 gcaccgcctg gctccctgga ggtgccggac ccatgagcgc gggctggaac agcagcgccg
      181 atgcgagggt ccagaccaga gagatcatga gcagcttgcc catgctggcg gtacccggat
      241 ggctcgaggc ccacaggctg ccgaagccgg tcgcggtcgc aagcgcgctg aaaaacaccc
      301 cgcgcgccag actcgaagtc agcagatgtg acccgcccga gcgccatccc atgacgaaat
      361 aaatgtgaaa cgccactccg ataccgaaca gcagcggcag ggcgatgatg ttggcaaagt
      421 tcagcggctg cccgatcagt acgcaggtac ccatggtaag gagaccggtc agcacaatgg
      481 gcgccatggt gatcgccaca tcgcgcaccc ggcgcagcgc aatcaacagc agcccgcaga
      541 tggcgacgaa tgacagcacg ccggcttcga tgaaggcgtc gaccaccgca cgcgcgtact
      601 cctgcaggta gatcgccgga ccggtagcgt cgggcgctat cttgagtccg gcggccgcga
      661 agcggcgcag caccgcatcg tcgttcgagt cgccgcgggg caataccgag acgcgtgcgc
      721 gcccatcggc ggtctgccag gcacgcacgg tctcctctgg cagcgtggca aggctgaccg
      781 gctgcggctg cagaaagctg cggatctgcg cgagtgtggt catgaagctc ggaatgagca
      841 tgtgcgcagc gccgccacgc gctgccgggg tgccacgggc gagcgcctcg agcgccacag
      901 caagccgctg cgtgtcggtc cgcaacgcct ggtcctgcgt ggagatctgc cgcagcttcc
      961 cggcggcgcg ctgcaggctg tcgatgagct ccgcatcggt cggcggcgcg gcgacctcga
     1021 ggggattcaa ggtgagatcc acgagattgg cggtatcggc gatgagcgca atcttgtgcg
     1081 cctgatcccc gggaatgaag ctcgagagcg tgtgcgcgct atggaccgtc gggttgccgc
     1141 ggaagaccgc cgccagctcg tcggccgcag cgaggctggg gcggaggacc tccagagtat
     1201 tcggggagcg ctccggatcg cgcagcagat cgacgagcgt ggcaacggac tcgaccgtgg
     1261 cggcgcgcag atggatggga ttgaaatcga acctcaccag tggcagcaat gccgcgcaga
     1321 tcagggccgc agccgcgccg ctgccgacca ccagcacccg gtggccgagc acgtaatcat
     1381 cgatcaccgc cagctgtgcc cctgctcgca gaggcacgcc gggcgcgcga atgagtgcga
     1441 tcagcgccgg caacagcgtg agattgagcc cgagcgcggt gaacatcccc acgcccgcta
     1501 tcaccccgag ctgcgacacg ccgtagtagg cagtcggcgt aaaggcgaga aagcccgcgc
     1561 cgatcgcggc cgccgccagg gtgagcgatt tccccatgct gcggccggtg gcgatgagag
     1621 cgcttcccag cgctcctccg gcggcacgtt ccgtgcgata acgcacactg aactgaatgc
     1681 cgaggtccat tcccatgccc acgaacaagg gaatgaaggc gacggagatg acgttgaagc
     1741 gctgaaataa cgcgagcccg agcgcagttg ccgtcagcag gcccaccaga gtcgttatca
     1801 gtatcgcggc aatgagacgg gccgagcgca ccgcgaacca gagcatcagc acgattgcca
     1861 cgctggcgag cacagcgatc aatccggcgc gttgcgtgag cgttgcgaat tcctcgtcct
     1921 gtaacggcac tggaccggtc aggcgcacgc tgaggccatg cgcagggtcg agctgcagtc
     1981 gctgcgccgt gctgcggatc gcatcgatcg gcaatttgcc gggttgcagg cgggtgaaat
     2041 cgaggcgcgg atcgacgagg acgatgtggc gcagctcgcg ctcgtcggcc tcgttaccgg
     2101 tgatcagcgt gcgccaggaa aagaacggtt gccggccggc ggtcaggcct gcgagcgcgt
     2161 ccgtcagtgc gcggatggga gtgcgcagct cctgcaccga gctttccccg ttgttcactc
     2221 ccttggcgat cagggaaagt gtattggcga gaccgcgtaa cgaggggtcg gaagccatgg
     2281 agccgaggaa cggttggacc ttcacgattt gagcgcagac tgtcttcacg tcctcggtcg
     2341 aagcgaagag caagcggttg cgagcccaga agggtccggc gtccggctgc tgcaccgagc
     2401 ggaacagtct tgtgtcggca acgagactcg cggcgagcgc gctggcgccc atcgcgctca
     2461 attccggcgt gcgcccatcg accaccacga cgatgtcggg gttccccagc gggaaggccg
     2521 cgttgaagtc tgcctggcgc acgcgccagg gaagcgttgg cgataacaac gcgcgcgtgt
     2581 ccgtggtcat ggcgaagtgg cccctgacat actgagccat cgcggagcac aggatgatta
     2641 ccaccgcgag cagcatccat gaatgcgcga ccgaccgacc gaccacacga gcaataagcg
     2701 aagactcgag gcgcggcagc atggacgtcg cgggcttgct cactcgcgga tccgtgggcg
     2761 cgtgcgcggc ggctgccgcg cccgtagcga cgcgagcg
//
LOCUS       DATDMP010000083         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_569318, whole genome shotgun sequence.
ACCESSION   DATDMP010000083 DATDMP010000000
VERSION     DATDMP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569318"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VI195_01390"
     CDS             complement(<1..248)
                     /locus_tag="VI195_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6823064.1"
                     /translation="MFKKILLAYDGSEPARKAFDRCIEMTRSFHGELSIVGVIRPPEF
                     AEDVETEALIDSARSRFDREIAELSPRAIAAGISPRITV"
     gene            complement(258..1481)
                     /locus_tag="VI195_01395"
     CDS             complement(258..1481)
                     /locus_tag="VI195_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6823065.1"
                     /translation="MTEIYGLATLWLGLALIATLLSIWLRIATAMSEIIVGTVAQLII
                     GAAIGASALGTDQSWVRFLASTGAVVLTFLAGAELDPQVMRREWRQATVVGLVAFAAP
                     FLGCAAVARYFIGWSVDASWLAGIALSTTSVAVVYAVMLEFGLNRTDYGKVVLAACFV
                     NDLATVLALGFKFSPFTFRTLIFVGGSCAAFAIMPWLVPRFFKRYGGRPSELEAKLLL
                     LMMFGLGALALWAGSEPVLPAYVLGMILAGAVGRDHALVRRLRTLTFGLLTPFYFIRA
                     GSYVSIPALIAGPGILLILLAAKMLSKFIGVYPVTKFFNSPPKEAMYTTLLMSTGLTF
                     GSISALFGLTHGVITQQQYSYLIAAVIGSAIVPTLVANAFFLPHHLVRSAAEGPQGES
                     IRTSSRVAANTPGAE"
     regulatory      complement(1663..1724)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1938..>2842
                     /locus_tag="VI195_01400"
     CDS             1938..>2842
                     /locus_tag="VI195_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY6823066.1"
                     /translation="MPQHTPPGPRGVPLLGDAIAFGRDPLRFLTECARRYGDLVAFRI
                     GAWPALLVNNPDDIEYVLVKNHRGFTKNRHFWRYVRAIFGTGLLTAVGDDWQRQRRLN
                     APAFSGQRLASYGAVMVRHAEAMLKSWRPGERVDVHAEIMALTLEIAAETLFGTSVRR
                     DIADIEHSTNVLMAEISARLRRPIFIPDAIPIPGNVRYRRALQRIDALVTRIVAERRR
                     SGEDRGDLLSLLLLARDENGEPMTERQIRDEVVTMLLAGHETTALALSWTCYLLSKYP
                     STESALAAEVRDVLGTRSPTVDDLVR"
BASE COUNT          565 a          887 c          895 g          495 t
ORIGIN      
        1 accgtgatgc gcggagagat gcccgctgca atcgcacgcg gcgacaattc ggcgatctcc
       61 cggtcaaagc gcgagcgtgc actgtcgatc agcgcctccg tttcgacatc ctcggcgaat
      121 tccggcggcc ggatcacccc gacgattgac agctccccgt gaaagctcct ggtcatctcg
      181 atgcatcggt cgaaggcctt cctggccggc tctgaaccgt cgtaggccag gagaatcttc
      241 ttgaacatgg ccaagaccta ttcggcgcct ggagtgttag ccgctacccg ggagctggtc
      301 cgtatgcttt caccctgtgg gccctctgcg gcagaccgca caagatgatg cggcaggaag
      361 aacgcattcg cgacgagcgt cggcacgatg gcgctgccga tcacggcagc gatcaagtag
      421 gaatactgct gctgcgtgat gaccccatgg gttaaaccaa agagcgccga gatgctgccg
      481 aaggtgagcc cggtcgacat gagcagcgtg gtgtacattg cttctttggg tggcgagttg
      541 aagaacttcg tgaccggata cacaccgatg aatttcgaca gcatcttcgc cgcgagcagt
      601 atgagcagga tacccggacc agcaattaga gcggggatcg aaacgtacga tccggcgcgg
      661 atgaaataaa acggcgtgag taaaccgaac gtcagcgtgc gcagccggcg taccagtgcg
      721 tgatcgcggc ccaccgcgcc ggcgaggatc atgccgagga cataggcagg cagaacgggt
      781 tcgctgcctg cccagagcgc aagtgcgccc aggccgaaca tcatcaggag cagcagtttg
      841 gcttcgagct ccgagggtcg gccgccgtag cgcttgaaaa atctcggcac cagccagggc
      901 atgatcgcaa acgccgcgca gcttccgccg acgaatatca gcgtccggaa ggtgaagggc
      961 gagaacttga accccagggc gagcaccgtg gccaggtcgt tcacgaagca ggccgccagc
     1021 accaccttgc cgtagtcggt tcggttgagc ccaaactcca acatcacggc gtagacgaca
     1081 gccaccgacg ttgtcgagag tgcgatgccg gcaagccagc tcgcatccac gctccagccg
     1141 atgaagtaac gagcgaccgc cgcgcagccg aggaacggag cggcgaaggc aaccaggccg
     1201 accacggtgg cctgccgcca ctcgcggcgc atgacctgcg gatcgagctc ggcgccagcc
     1261 agaaaggtca gaacgaccgc gcccgtgctc gcgaggaaac gcacccagga ctggtcggtg
     1321 ccgagcgcgc tcgcaccgat tgccgctccg atgatcagtt gagcgacagt ccccacgatg
     1381 atttctgaca tcgcggtggc gatccgcagc catatagaca gaagtgtggc gatcagagcc
     1441 aggcccagcc acaaggtcgc gaggccgtag atttcggtca atcgggtacc tgctccggta
     1501 agccttcttg accgtcatcg agccgcagca ggggtggcga gaggcacagg tcaggtgcag
     1561 gcatccagaa gttcgaatcc cgtgcgacgt cgaccggaga acggccgcac cgaaggagtg
     1621 gtgggctgtc gcacgtacgc ctaccgcttt gcccttcgag ggcgttgggt aggcatcatc
     1681 agccttacgg cggttcgcgg aggaatgcca tctccggcaa tgctgcgatc ttagcggagt
     1741 cagagttgat cggctacctc gagcagcggc gcgcactgct aatgccgact cctgctcagg
     1801 cgcgtggagc tcgtgtcggt gtcggcccac agctcgccac gctcacactc ttctcggcca
     1861 ctgcgcagag cgcgcagaat tgcccagtgt cgaccattgg ctggccggcc tgctcttaaa
     1921 gcatcgcttc aatctctatg ccacaacaca cgccaccggg ccccagggga gttccgctcc
     1981 tgggggatgc gatcgcgttt ggccgtgatc cgctgcgatt tctcaccgag tgtgctcgcc
     2041 gctatggcga tctagtggca ttccgcatcg gcgcgtggcc agcgctgctc gtgaacaatc
     2101 cggacgacat cgagtacgtg ctggtcaaga accatcgcgg cttcaccaag aatcgacact
     2161 tctggcgcta cgtgcgcgcc attttcggca ctggcttgct cactgcggtc ggcgatgatt
     2221 ggcagcgaca gcgtcgcctc aacgcacccg ccttcagcgg ccagcgcctg gcaagctatg
     2281 gcgcggtcat ggtgcgtcac gccgaggcca tgctgaaaag ttggaggccc ggcgagcggg
     2341 tggatgttca cgccgagatc atggcgctga ccctggagat tgctgcagaa accctgttcg
     2401 gcaccagtgt ccggcgcgat atcgccgata tcgagcactc gaccaatgta ttgatggccg
     2461 agatctcggc ccgtttgcgc cgacccatct ttatccccga cgccatcccc attcccggca
     2521 acgtgcgcta ccgccgggcg ctccagcgga tcgatgcatt agtcacccgg atcgttgctg
     2581 agcggaggcg aagtggtgag gaccgcggcg atctgctctc gctgctgctc ttggctcgtg
     2641 atgagaatgg cgagcccatg accgagcggc agatccgtga cgaggtcgta acgatgctgc
     2701 tcgccgggca cgagaccacg gcattagcgc tatcgtggac gtgctatctg ctatcgaagt
     2761 atccgagcac cgaatccgcg ctcgccgctg aagtgcgcga cgtcctgggg acacggtcac
     2821 cgaccgtgga tgatctcgtg cg
//
LOCUS       DATDMP010000084         3112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_347158, whole genome shotgun sequence.
ACCESSION   DATDMP010000084 DATDMP010000000
VERSION     DATDMP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347158"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3112)
                     /locus_tag="VI195_01405"
     CDS             complement(<1..>3112)
                     /locus_tag="VI195_01405"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY6823067.1"
                     /translation="ALSNDIHRNLDIPLAGIRKELRAQGLADVVLRSWVRTGDTPQAE
                     RQRAAKLPPHIVVTTPESLYILLGSDSGRAMLKTTRAVIVDEIHAVASSKRGSHLTLS
                     LERLSALCGGKLQRIGLSATQKPIEEVASFLVGAGTDHKQPLIVDSGHYRTRDLQLEV
                     PPSPLEAVMSAEIWDQVYDRLAELIEGHRTSLIFVNTRRMAERVTRNLSDRLGEARVA
                     THHGSLAKEQRLDAEQRLKSGALSAMVATSSLELGIDIGDVDLVCQLGSPRSIAAFLQ
                     RVGRSGHSVGGQPKGRLFPLSNDDLVECAALIDSANHGELDRLIMPQAPVDVLAQQIA
                     AEVNAQEWDEGQLFGLVQHALPYCNLERETYASVLRMLANGFTTRRGRRGALIHRDEI
                     NGVLRGRRGARLTAITSGGTIPDNSDFQVLLEPENVVVGTVNEDFAVESLQGDIFQLG
                     NTAYRIIRVERGVVRVEDAQGQPPTIPFWLGEAPARTDELSQSVSRFRSEIERRLNDG
                     EAVSALGFLVDDLRLDRAAAEQIVDYLGAAYRALGALPTLETLVFERFFDEAGGMQLV
                     IHSPYGSRINRAWGLALRKRFCRKFNFELQAAATEDNIVLSLTTAHSFELADVARYLN
                     PKTVRDLLIQALLDAPMFGTRWRWVAGVALALPRFRGGKKVPPQLIRMMSEDLVGTIF
                     PDQVACAENLVGEREVPDHPLVAQTISDCLHEAMDIDGLERLLADIESGKIRIIARDL
                     TEPSPLAREVLTARPYAFLDDAPLEERRTQAVMSRRWLDPDTADEIGRLDEAAIARVR
                     EEAWPDAAHADELHDALVWFGFLTEAEADARENWKESLIQLAQSRRVALFTAPEAMFW
                     IPAERLRQVEAVYPGAVVEPAIAAPANSDQNWQQDAALVELVRGRLEGQGPTTLAQLA
                     GTFGLPPERISAALTALELEGFALAGSFTPGGSEREWCERRLLTRIHRYTVKRLRAEI
                     EPVSARDYLRFLFNWQGVSPDTRREGAQALVAVVEQLAGFEAPAAAWESAILPARLSD
                     YEPSLLD"
BASE COUNT          576 a          973 c         1039 g          524 t
ORIGIN      
        1 catcgagtag gctcggctca taatcggaaa gccttgcggg caggatcgcg ctctcccatg
       61 cggcagctgg cgcttcgaag ccagcaagct gctcgactac agcaacaagc gcctgcgctc
      121 cttcgcgtcg ggtatcgggc gagacgccct gccaattgaa caagaagcgc aggtaatcgc
      181 gcgcggagac aggctcgatc tcggcgcgga gccgtttcac cgtatagcgg tgaatgcggg
      241 tgagcagccg ccgctcgcac cattcccgct cgcttccgcc cggagtgaag gagccggcaa
      301 gggcgaagcc ttcgagttcg agtgcagtga gtgcggcgga aattcgctct ggcggaagcc
      361 cgaatgtgcc tgcgagctga gcaagcgtcg taggcccttg gccttcgagc cgcccgcgca
      421 ccagctcgac caaagcagcg tcttgctgcc aattttggtc ggaattggcg ggcgccgcga
      481 tggcgggctc gaccacggca ccaggataaa ctgcctcgac ctgccgcagc cgctcggccg
      541 ggatccagaa catggcctcc ggcgcggtga acaacgcaac acggcgagat tgcgcgagct
      601 gaatgagcga ctccttccag ttctcacgcg catcggcttc tgcctcggtg aggaagccaa
      661 accagaccag cgcgtcatgc aactcatcgg cgtgggcggc gtctggccac gcctcctcgc
      721 gcacgcgtgc gatcgccgcc tcgtcgaggc ggccgatctc gtcggcggtg tccggatcga
      781 gccaacggcg gctcatcacc gcttgcgtgc ggcgctcttc taaaggcgca tcgtcgagaa
      841 aggcataagg ccgagcggtg agtacctcgc gtgcaagcgg cgaaggctcg gtcagatcgc
      901 gggcgatgat cctgatcttg cctgactcga tatctgcgag caggcgctcc agtccgtcga
      961 tatccattgc ctcatgcagg cagtcggaaa tggtctgcgc caccagcgga tggtctggca
     1021 cctcgcgctc gccgacgaga ttttcggcgc aggcgacttg gtccgggaag atcgtgccga
     1081 caagatcttc ggacatcatg cgaataagct gcgggggcac cttcttccca ccgcggaagc
     1141 gcggcagggc aagcgcaacg ccggctaccc agcgccaacg cgtgccgaac atcggcgcat
     1201 caagcaatgc ctgaatgagc aggtcgcgca cggtcttcgg gttgagatat cgcgcgacat
     1261 ccgcgagctc gaagctgtgc gcagtggtca gtgacagcac aatgttgtct tccgttgcag
     1321 ccgcttggag ctcgaaattg aacttgcggc aaaagcgctt gcggagcgcg agcccccagg
     1381 cgcggttgat gcggctgcca tagggcgagt ggatcacgag ctgcatgccg cccgcctcat
     1441 caaagaaacg ctcgaagacg agcgtctcga gcgtcggcag cgcgccaagc gcgcgatagg
     1501 ccgcacctaa gtagtcgacg atctgctcgg ccgctgcgcg gtcgagacga aggtcgtcca
     1561 cgaggaatcc gagggcgctt acggcctcgc cgtcgttgag gcggcgctca atctccgaac
     1621 gaaagcgcga gacggattgg gagagctcgt cagtgcgtgc cggggcttcg ccgagccaga
     1681 acgggatcgt cggcggctga ccttgcgcgt cctccacgcg caccacgccg cgctcgacgc
     1741 ggatgatgcg ataggcagtg ttgccgagct gaaatatatc gccctggagg ctctccacgg
     1801 caaaatcctc attgaccgtg ccgaccacca cgttctcagg ctcgagcaga acctggaaat
     1861 cggaattgtc ggggattgtg ccgccagagg tgatcgccgt gagccgagcc ccacgccgcc
     1921 cgcgcaatac gccgttgatc tcgtcgcgat ggatcagggc gccgcgccgc ccgcgccgcg
     1981 tggtgaaacc attggcgagc atgcgcagta ccgaggcata ggtttcgcgc tccaagttgc
     2041 agtaaggcaa ggcgtgttga acgaggccga agagttggcc ctcgtcccac tcctgcgcat
     2101 tgacttccgc cgcgatctgc tgggcgagca cgtcaactgg cgcctgcggc atgatcagcc
     2161 ggtcgagctc gccgtgattg gcactgtcga tcagcgcggc gcattcgacg agatcgtcgt
     2221 tggagagggg gaataggcgc ccctttggct gtccgccgac gctgtggcct gaacggccga
     2281 cccgctggag gaaagccgcg atcgagcgtg gcgaaccaag ctggcagacg agatctacat
     2341 cgccgatgtc gatgccgagc tcgagcgagg aggtggcaac catggcggaa agcgcgcctg
     2401 acttcaggcg ttgctcagca tcgagacgct gctctttggc aaggctgccg tggtgcgtgg
     2461 cgacccgcgc ctcgccaagc cggtcagaga ggttgcgcgt gacgcgctcg gccatgcgcc
     2521 gcgtgttgac gaagataagc gaggtgcggt ggccttcgat cagctcagcg agccggtcat
     2581 agacctgatc ccagatctcc gctgacatca cggcttcgag aggggaaggg ggcacttcga
     2641 gctgcagatc gcgcgtacga taatgtccgc tgtcgacgat tagcggctgc ttgtggtccg
     2701 tgccagcgcc gaccagaaaa ctcgcgacct cctcgatcgg cttctgagtg gcagacagcc
     2761 cgatccgctg cagtttgccg ccgcacagcg cggatagccg ctcgagcgac agcgtaagat
     2821 gcgagccacg cttgctcgag gccacagcgt ggatctcgtc cacgatcacc gcccgcgtgg
     2881 tcttgagcat ggcgcggcct gagtcggagc cgagcaggat gtagagcgac tccggcgtgg
     2941 tcaccacgat gtgcggcgga agcttggcgg cacgctggcg ctcggcttgc ggcgtgtcgc
     3001 cggtgcgcac ccaagagcgg agcacgacat cagccagtcc ctgcgcgcga agctccttgc
     3061 ggatgccggc gagcggaatg tcgagattgc ggtggatgtc gttcgatagc gc
//
LOCUS       DATDMP010000085         3320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_569598, whole genome shotgun sequence.
ACCESSION   DATDMP010000085 DATDMP010000000
VERSION     DATDMP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569598"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VI195_01410"
     CDS             complement(<1..658)
                     /locus_tag="VI195_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY6823068.1"
                     /translation="MSTARRLGVVMDPIGAIAYAKDSTLAMLLAAQRRGFTLAYLEQR
                     DLMLRDGTAFGRIRPLSVRADPKGWFTLGEPAIEPLGALDCLLMRKDPPFDTEYIYSS
                     YILERAESAGVLVVNRPQGLRDINEKVYTAWFPQCCTATLITRDMADMGAFLREHRKI
                     VVKPLHGMGGRSIFVLADGDPNARVVFENLTDYGQRFAIAQRYLPEIATSGDSRVLLI
                     D"
     gene            980..1372
                     /gene="pilG"
                     /locus_tag="VI195_01415"
     CDS             980..1372
                     /gene="pilG"
                     /locus_tag="VI195_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HEY6823069.1"
                     /translation="MSGNNAKLQGLKVLVIDDSKTIRRTAETLLAKEGCEVYTAIDGF
                     DALAKIADHQPDIVFVDIMMPRLDGYQTCSLIKHNKVFRSIPVIMLSSKDGLFDRARG
                     RIVGSEHYLTKPFTKDELLSAIETHIGR"
     gene            1378..1743
                     /locus_tag="VI195_01420"
     CDS             1378..1743
                     /locus_tag="VI195_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6823070.1"
                     /translation="MPLILIVDDSPTEVHVMQKALEKHGYRTAAAADGAEGVRLAREM
                     TPDLIFMDIVMPGMNGYQATRALANDPRTRAIPIIMVTSKGQETDRIWGLRQGAVDYM
                     VKPVSPEQLVAKAQATLAP"
     gene            1751..2329
                     /locus_tag="VI195_01425"
     CDS             1751..2329
                     /locus_tag="VI195_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY6823071.1"
                     /translation="MSEPTPTPEIELKSLRDRPFELLAELERRGRALTAHLTDQSTAG
                     REWVGVALRMAGDLYLVAREETREVLGVPAGTTRVPGAKSWIKGLANVRGQLLPIVDL
                     RHFLGSGVTPVTRNTRVLVVNHREIPAGLLVDEVLGFRRFAENEFTGDAPPTVARCER
                     YLAGAFRRAEEQWPVLSLRSILESPAFMEAAA"
     gene            2326..>3320
                     /locus_tag="VI195_01430"
     CDS             2326..>3320
                     /locus_tag="VI195_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6823072.1"
                     /translation="MTATAPTPARTPKSLTPLLGGAVLLAAGSAAAAWFTLNLSAPLT
                     WAPLGLSAAVLVLLLIALVAAARTRRTVERQRASAEAQIRENDRNQQAILRLLDELSN
                     LADGDLTVQATVTEDITGAIADSINYAVDALRGLVTTINASAIQLDSATRQTQALSQH
                     LAKASGAQSKQIASATESAASMAGSVEEVSGNAERASDVARHSVEVAHKGGDAVRRTI
                     DGMNTIRETIQETSKRIKRLGESSQEIGNIVELINDIAEQTNILALNASIQSSMAGDA
                     GRGFAVVADEVQRLAERAANATKQIEVLVRTIQTDTNEAVVSMERSTTDVVGGALL"
BASE COUNT          561 a         1148 c         1117 g          494 t
ORIGIN      
        1 cgtcgatgag cagcacccgt gaatcgcccg aggtcgcgat ctcgggcaga taacgctgcg
       61 cgatcgcaaa gcgctggcca tagtcggtca ggttctcgaa caccacgcgt gcgttggggt
      121 cgccgtcggc gagcacgaag atcgagcgcc cgcccatgcc gtgcagcggc ttgacgacga
      181 tcttgcggtg ctcacgcagg aacgcgccca tgtcggccat gtcgcgcgtg atcagcgtcg
      241 cagtgcagca ctgcgggaac caggcggtgt agaccttctc gttgatgtcg cgcagtccct
      301 ggggacggtt gaccaccagc acccccgcac tctcggcccg ctcgaggatg tagctgctgt
      361 agatgtactc ggtatcgaac ggtggatcct tgcgcatcag cagacagtcg agcgcgccga
      421 ggggttcgat cgccggctcc ccgagcgtga accagccctt cgggtcggcc cgcacgctca
      481 ggggtcggat gcgcccgaag gcggtgccgt cgcgcagcat cagatcgcgc tgctcgaggt
      541 aagcgagggt gaagccgcga cgctgcgcgg cgagcagcat cgcgagtgtc gagtccttcg
      601 cataggcgat ggcgccgatc ggatccatca ccacgccgag tcgtctggcc gtgctcatcg
      661 gctcccgtcc gagtgcgccg caggggcgga ttatgctcag tgcggcacga atatgtctgc
      721 cccgtgcaca gtaccggcgg aaagggctcg ggagtacccc gccggcttca aaaaacgtga
      781 cgtgcatggc atagcagggg gtgtaccgat atgttaaaac ccgcctgcgc ttgccctctc
      841 gcatgggggg cccgtgagac tgcggcgccg ggtggacggc gcgcgctgcg cccgtgtgtt
      901 caggggccct cacggcgatt cccgaacgcg cgctgcgcct gcaaagagcg cggtctgtaa
      961 cggaaggagt tgcctggccg tgagcggcaa caacgcgaaa ctccagggtc tcaaggtgct
     1021 ggtcatcgac gacagcaaga ccattcggcg caccgccgag acgctgctcg ccaaggaggg
     1081 ctgcgaggtc tacaccgcca tcgatggttt cgatgcgctc gccaagatcg ccgaccatca
     1141 gcccgacatc gtatttgtcg acatcatgat gccgcgcctc gacggctatc agacctgctc
     1201 cctgatcaag cacaacaagg tattccgctc cattccggtg atcatgctgt cttccaagga
     1261 cggcctgttc gatcgcgccc gcggccgcat cgtcggctcc gagcactacc tcaccaagcc
     1321 cttcaccaag gacgagctgc tgtccgcgat cgagacgcat atcgggagat gacggcgatg
     1381 cccctgatcc tgatcgttga cgattcaccc accgaagtgc acgtcatgca gaaggcgctc
     1441 gagaagcacg gctatcgcac ggccgcggcc gccgacggcg ccgagggcgt gcgtctcgcg
     1501 cgcgagatga ccccggacct catcttcatg gacatcgtca tgccgggcat gaacggctac
     1561 caggcgacgc gcgcgctggc gaacgatccg cgcacccgcg ccattcccat catcatggtc
     1621 acctccaagg gacaggagac cgaccgcatc tggggcctgc gccagggcgc ggtggactac
     1681 atggtgaagc cggtgtcgcc cgagcagctg gtcgccaagg cgcaggcgac gctcgccccc
     1741 tgagaagccc gtgagcgagc cgacccccac ccccgagatc gagctgaaga gcctgcggga
     1801 ccggccgttc gagctgctcg ccgagctcga gcggcgcggc cgtgcgctca ccgcgcacct
     1861 caccgaccag agcaccgccg ggcgcgagtg ggtcggggtg gcgctgcgca tggcgggaga
     1921 cctgtacctg gtggcgcgcg aggagacccg cgaggtgctc ggcgtgccgg ccggcacgac
     1981 gcgcgtgccg ggagcgaagt cctggatcaa gggtctcgcc aacgtgcgcg gccagctgct
     2041 ccccatagtc gacctgcggc actttctcgg cagcggcgtg acgccggtga cgcgcaacac
     2101 ccgcgtgctg gtggtgaacc accgcgagat tcccgccgga ctcctggtcg acgaggtgct
     2161 cggtttccgg cgctttgccg agaacgaatt cacgggcgat gcgccgccga cggtggcgcg
     2221 ctgcgagcgc tacctcgcgg gggccttccg ccgtgccgag gaacagtggc cggtgctctc
     2281 gctgcgctcg attctcgaga gcccggcgtt catggaagcc gcggcatgac ggcgaccgcg
     2341 cccaccccgg cccgcacgcc gaagtcgctc acccccttgc tgggcggggc ggtgctgctc
     2401 gccgcgggca gcgcggcggc cgcgtggttc acgctgaatc tctcggcgcc gctcacctgg
     2461 gcgccgctcg gcctctcggc agccgtgctg gtgctgctgc tgatcgcgct ggtggcggcg
     2521 gcgcgcaccc gccgcacggt ggagcgccag cgcgcttccg ccgaggcgca gatcagggag
     2581 aacgaccgca accagcaggc gatcctgcgc ctgctcgatg agctctccaa cctcgccgac
     2641 ggcgatctca ccgtgcaggc gacggtcacc gaggacatca ccggcgcgat cgccgattcg
     2701 atcaattacg ccgtggatgc gctgcgcggc ctggtcacca ccatcaacgc ctcggccatc
     2761 cagctcgact ccgccacccg tcagacccag gcgctgtcgc agcatctcgc caaggcctcg
     2821 ggcgcgcagt cgaagcagat cgcctccgcc accgagtccg cagccagcat ggcgggctcg
     2881 gtggaagagg tgtcgggcaa tgccgagcgc gcctccgacg tcgcgcgtca ctcggtcgag
     2941 gtggcgcaca agggcggcga tgcggtgcgg cgcaccatcg atggcatgaa caccatccgc
     3001 gagaccatcc aggagaccag caagcgcatc aagcgcctcg gcgagagctc ccaggagatc
     3061 ggcaacatcg tcgagctcat caacgacatc gccgagcaga ccaacatcct ggcgctgaac
     3121 gcctcgatcc agtcctcgat ggccggcgac gccgggcgcg gcttcgcggt ggtcgccgac
     3181 gaggtgcagc gcctcgccga gcgcgccgcc aacgccacca agcagatcga ggtgctggtg
     3241 cgcaccatcc agaccgacac caacgaggcg gtggtgtcga tggagcgcag caccaccgac
     3301 gtggtgggcg gggcgctgct
//
LOCUS       DATDMP010000086         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1569583, whole genome shotgun sequence.
ACCESSION   DATDMP010000086 DATDMP010000000
VERSION     DATDMP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569583"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VI195_01435"
     CDS             complement(<1..826)
                     /locus_tag="VI195_01435"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY6823073.1"
                     /translation="MKRTSGIEWKWPLSPQAGSITTIEMHTGGEPLRVITGGLPPLAG
                     RTVLEKRRYFREHFDHLRTGLMWEPRGHADMYGAVITPSQEADFDVFFLHNEGYSTMC
                     GHAIIALTTLVLEAGLAPHAASLEHSSADGELPLSFSVPAGLIRARAALRDGKVRHVS
                     FRNVPSFLYARDRQVEVAGIGRVRFDVAYGGAFYAIVEAAPLGLRLTPEHVDRLIEHG
                     RRIKQAVMRAVTIAHPYEQELGFLYGTIFTGAAQHPGSHSRNVCVFAEGELDRSATG"
     gene            complement(823..2949)
                     /locus_tag="VI195_01440"
     CDS             complement(823..2949)
                     /locus_tag="VI195_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY6823074.1"
                     /translation="MGRIERLLAGMTVAEKIGQLNMAPSFRAVTGPGVRRELEKDIRS
                     GRVGNVLNLWGRAQVHALQRLAVEGSRLGIPLLVGLDVIHGHRTIFPVPLAEAGSFDP
                     DIWERTARAAAAEAAADGVTLTFAPMVDVARDARWGRVVESPGEDAWVACAFAAAKTR
                     GFQGGELSGAGGVAATAKHLCGYGAVTAGREYASVELSERTLHEVYLPPFAAAVAAGA
                     AALMPAFIDIAGVPMTANVSLLQDWLRDRQAFDGLVISDYNAIAELCEHGVAANPAEA
                     AALALSAGVDIDMASEVYAQGLPEALARGLISPAQLDASVLRVLRLKERLGLFDDPYR
                     DAPPPNLTVPAHTLAREAARRAIVLLTHRRPVLPLSANLRSVALIGPLAQASQEMLGP
                     WPAAGEPEEAVSILAGLRSALPHCRIETVAGVDIERRDLGGIPAALECSRAADLTILC
                     LGEPASMSGEGGSRADLSLPGDQPALAQAALELGKPLIALLTCGRPLTAPWLFERADA
                     VLVTWFLGSEAGHAIADVLTGKFNPSGKLAVTWPRHVGQVPIFYGQRPTGRPAKVGER
                     LTSSYLDVAPTPQFPFAHGLSYSTFRLYDLSCSPRTVATSGAIDVAVSLHNDSAVRGE
                     AKVFLFVHDPIASLSRPLLELRGVRCIALGSGERGSVSWQLPVSSLAFVAADLAPVVE
                     PGRFEIYVGLSADPHALVGCAIEVVP"
BASE COUNT          538 a         1099 c         1049 g          488 t
ORIGIN      
        1 agcccgtcgc cgagcggtcg agctcgcctt cggcgaacac gcagacgttg cggctgtgcg
       61 atccggggtg ctgcgcagcg ccggtgaaga tcgtgccgta aaggaagccg agctcctgct
      121 cgtatggatg cgcgatcgtg actgcgcgca tcacggcttg cttgatgcgc cgtccgtgct
      181 cgatgaggcg atcgacgtgc tccggggtga gccggagccc gagcggcgcg gcttccacga
      241 tggcatagaa cgcaccgccg tacgccacat cgaagcgcac ccggccgata cccgcgacct
      301 cgacctgccg atcgcgcgcg tagaggaacg atgggacatt gcggaaactg acgtgccgca
      361 ccttgccgtc gcgcagcgcc gcccgtgcac ggatgaggcc cgccggaacg gaaaaactca
      421 gtggcagctc gccatcggct gagctgtgct cgaggctcgc agcgtgcggc gcgaggccag
      481 cctcgagcac gagcgtcgtg agggcgatga tggcgtgacc gcacatcgtg ctgtagccct
      541 cgttgtgcag gaagaacaca tcgaagtccg cctcctgcga gggcgtgatc acggccccgt
      601 acatgtccgc atgtccgcgc ggttcccaca tcaacccggt gcgcagatga tcgaaatgct
      661 cgcggaaata acggcgcttc tccagcaccg tcctgcctgc gagcggcggc aggccgcccg
      721 tgatcacgcg cagcggctca ccgccagtgt gcatttcgat ggtcgtgatg ctgcccgcct
      781 gtggcgacag cggccatttc cactcgatgc cggacgttcg cttcatggca cgacttcaat
      841 ggcgcagccg accagtgcgt gcgggtctgc actcaacccg acgtagattt cgaagcggcc
      901 cggctctacc accggagcga gatcggcagc gacgaacgcc agcgaggata cgggcaactg
      961 ccagctgaca cttccgcgct ctccggatcc gagtgcgatg caccgcacac ctctgagctc
     1021 gaggagcggt cgcgagaggc tggcgatcgg atcgtgcacg aatagaaaca ccttggcctc
     1081 gccgcgcacg gcgctgtcat tgtgcaacga cacggcgaca tcgatagccc cgctggttgc
     1141 tacggtgcgc gggctgcagc tgaggtcgta cagtctgaag gtgctgtacg agagcccgtg
     1201 cgcgaaggga aattgcggcg tgggcgcaac gtcgaggtat gagcttgtga gccgctcgcc
     1261 caccttcgcc ggccggccgg tcggccgctg accgtagaag atcggcacct gcccgacgtg
     1321 gcgcggccag gtgaccgcca gcttcccgct gggattgaac tttcccgtca gtacatcggc
     1381 gatcgcatga ccggcttcgc tgccgaggaa ccaggtcacg aggaccgcgt ccgcgcgctc
     1441 gaacagccac ggtgcggtga gcgggcggcc gcaagtcagc agcgcgatga gaggcttgcc
     1501 gagctcgagc gccgcctgcg ccagtgccgg ttgatctcca ggcaggctga gatctgcacg
     1561 actgccgccc tcaccgctca tggaggccgg ctcgcccaaa cagagaatcg tcaggtctgc
     1621 tgcgcggctg cactcgagcg ctgcgggaat acccccgagg tcgcgccgct cgatgtcgac
     1681 gcccgcaacc gtttcgatgc ggcagtgagg caatgcggaa cgcagtcccg cgagaatgct
     1741 caccgcttcc tcgggctcgc cggcggcagg ccatgggccg agcatctctt ggctcgcctg
     1801 cgccagcgga ccgatgagag cgacactgcg gagattcgca gaaagaggca gcaccggacg
     1861 gcgatgggtc agaagcacga tggctcgccg ggccgcctcg cgcgccaacg tgtgcgccgg
     1921 gactgtgagg ttgggcggcg gcgcgtcccg gtagggatca tcgaagaggc cgagtcgctc
     1981 cttcagccgc agcacccgca gcacactcgc atcgagttgc gccgggctga tcagaccgcg
     2041 cgcgagcgct tcgggcagtc cctgcgcata gacttcgctc gccatgtcga tgtcgacccc
     2101 cgcgctgaga gccagcgctg cggcctcggc ggggttggcg gccacgccgt gctcgcacag
     2161 ctctgcgatg gcgttgtagt cgctgatcac caggccatcg aatgcctgcc ggtcacgcag
     2221 ccaatcctgc agcagcgaga cgttggcagt catcggcaca ccggcgatat cgatgaaggc
     2281 gggcatgagt gcggcggcgc ccgccgccac ggcggcggcg aaaggtggca ggtacacctc
     2341 gtgcagcgtg cgctccgaga gctcgaccga ggcgtattcg cgccccgcgg ttaccgcacc
     2401 gtacccgcac aggtgcttcg cggtcgccgc gacgccgccc gcgccgctca actcgcctcc
     2461 ctggaaacca cgcgtctttg ccgctgcgaa tgcgcacgct acccaggcat cctcgcccgg
     2521 actttccacc acgcgccccc agcgcgcgtc gcgcgcaaca tccaccatcg gcgcaaacgt
     2581 cagggtgacg ccatcggcag cggcctccgc ggcggccgcg cgcgcggttc gctcccagat
     2641 gtcagggtcg aacgacccgg cttccgccag cggcacgggg aaaatcgtgc ggtggccgtg
     2701 gatcacatcg agtccaacga gcagcgggat gccaagacgt gagccctcga ctgcgagacg
     2761 ctgcagcgcg tgcacctgcg ccctgcccca caggttcagc acgttgccca cgcgtccgct
     2821 gcgaatgtcc ttctcgagct cgcgccgcac tccgggaccg gtgaccgcgc ggaacgatgg
     2881 cgccatgttg agctgaccga tcttctccgc gaccgtcatg ccggcgagca atcgttcgat
     2941 gcgtcccatg ctgttgcctt gcgctaagct gagctggatg ccgaatgtcc gtggtgactg
     3001 ctcccgcccg gatcgttcca cccagcggag ccggaacgga tcgatacgat gacgcaaccc
     3061 ccgagcgagt cctcccccgg taccggcggc gcgggcccgc ccgcgacgcc tgcccagacc
     3121 gcagactccc ttgcggcagc tgcgctcacc aaggataacc tcttcactga agtc
//
LOCUS       DATDMP010000087         2960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_14673, whole genome shotgun sequence.
ACCESSION   DATDMP010000087 DATDMP010000000
VERSION     DATDMP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14673"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..539)
                     /locus_tag="VI195_01445"
     CDS             complement(366..539)
                     /locus_tag="VI195_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823075.1"
                     /translation="MALLKPRTVLLNGIAHERSEIRSFALELDVATSNACDIEKIIDQ
                     VHEPVRLPMRKSH"
     gene            593..1462
                     /locus_tag="VI195_01450"
     CDS             593..1462
                     /locus_tag="VI195_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6823076.1"
                     /translation="MRLEQIVSNLLENAIKYTEPGGRIVVSLSREGDEVALRVRDNGI
                     GLRPQDVEEIFDLFTQIDTTLARSGGGLGIGLTVVRRLVALHQGRIEVRTAGLGRGSE
                     FIVRLPVAQRKAHPAQQPSPTAATAASSSQRVLIVDDYADSAESLALLVRSWGHEVTT
                     ARDGPAALALVERFAPDIALLDIGLPRMNGYELARQLRALARHRPLRLVAMTGYGREE
                     DRRASRAAGFDLHVIKSPLYRSTPHASHRVRGSAALSAHVHADRAIRASALLAIDAGH
                     PGSAGAGAGSRHA"
     gene            complement(1503..>2960)
                     /locus_tag="VI195_01455"
     CDS             complement(1503..>2960)
                     /locus_tag="VI195_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6823077.1"
                     /translation="FFACAAVMFVEPARSARARGALLHALGARNFEYFCGCLAFIRTA
                     HYWTMLHPEIESEEDMLSLLGAHEDLAKLLLEDPEADRCEMGERLYEELSALRELHER
                     QELERAKQALEEKDRQKDQFIAVLAHELRNPLAAIRSAADAMKLVGSADERSVRLLQR
                     LDRQSTAMARMLDDLLDASRIALGKVEVELKTLDLVNLLQEVLEEQQSRVREAGLMLE
                     VSIEEKPPSCVKADQVRLRQIIDNLLSNAIKFTPAGGAIRVRLQREGSLACITVRDTG
                     VGFDEQFASELFEPFTQEEHGPGRPGGGLGLGLAIASRLARLQGGSLRGESDGPSQGA
                     IFTLRIPLAPELNEGHTAVRTASQRQRGGGQVLLLVEDNVDVADNFAELLELSGFQVQ
                     VVHTGADAMRAALDCIPDVILCDLGLPGDMDGFAVASACRANDTLRSVRLIAASGYSS
                     REHQAAAKAAGFELLLTKPLTSASLGLLAGERRDQ"
BASE COUNT          535 a         1004 c          883 g          538 t
ORIGIN      
        1 tcggccaacc tcggaagcag atccgccaca gctgacaccg ctggtgcggc ggaagtagac
       61 atcagggccg cccactcggc ttcagccgat tcgagtttcg ttttcaaggt cggtgacagc
      121 aaccctgttg ccactgcggc caccatatgc tcaatctcag cacccagcgc aacgaggcgc
      181 ttggcgcgag tatcctcctg cgcggaccgc gcggcgcgtt actgcctgag agcctcactg
      241 accgctcgct tgaactccgc aacgtactca ggtccgctca gctcagcttt aattcctgcc
      301 agcagtttcg actcggctac cgcgcgtgcc actcgcagcg tgttcgtgca cgcgtgttta
      361 ccccgttagt gcgacttgcg catcggcaga cgcaccggct cgtgcacctg gtcgatgatc
      421 ttctcgatat cgcacgcatt actagtggcc acatcgagct caagcgcgaa actgcgaatt
      481 tcacttcgct cgtgcgcgat gccattgagg agaaccgtgc gcggcttgag cagcgccaac
      541 acacgctcac cgtcgagctc cccgaagcgt cctcgaggtc gagggcgatt cgatgcgtct
      601 ggagcagatt gtctcgaacc ttctcgagaa cgcgatcaaa tacaccgagc ccggcgggcg
      661 cattgtcgtg agcctaagca gagaaggcga tgaggttgcc ttgagggtac gcgacaacgg
      721 tatcggcctc cgcccgcagg atgtggagga gatcttcgat ctattcaccc agatcgacac
      781 caccctggcc cgctcgggcg gaggcctcgg gatcggcctc acagtcgtgc gacggttggt
      841 ggcgctgcac caggggcgca tcgaagtgcg caccgctggc ctcggccgcg gcagcgagtt
      901 catcgtccga ttgcccgtgg cgcagcgcaa ggcgcacccg gcgcagcagc cgagccctac
      961 cgccgcgaca gccgcctcgt cctcccagcg tgtgctcatc gtcgatgact acgcggactc
     1021 cgccgagtcc ctcgcgctgc tggtgcgcag ctggggtcat gaagtaacca ctgcgcgcga
     1081 cggccccgcc gccctggccc tggtcgagcg ctttgcgccc gatatcgcgc tcctggacat
     1141 cgggctgccg cgcatgaatg ggtacgagct cgcgcgtcag ctgcgcgcct tggcgcgcca
     1201 tcggccgctg cgtctggtgg cgatgacggg ctacgggcgc gaagaggatc gccgcgcctc
     1261 gcgagccgcc ggcttcgacc tgcacgtcat taaatcccct ttatatcgaa gcactccgca
     1321 cgcttctcac cgagtccgag ggtccgctgc cctaagcgcg catgtgcacg ccgaccgcgc
     1381 cattcgggca agcgcacttt tggccatcga cgcgggccat cctgggagcg ctggggcggg
     1441 ggccggatcg aggcacgcct aggccggcac cgaccgattt cacccggtcg cgtctgttca
     1501 gattactggt cgcgccgttc tccggccagc aagccgagcg aggcggaagt tagcggcttg
     1561 gtcagcaata actcgaaacc ggcagccttt gcagccgcct gatgttcccg cgaactatac
     1621 ccggatgccg cgatcagtcg cactgagcgc agagtgtcat tcgcccggca ggcgctcgcc
     1681 acggcaaagc cgtccatgtc tcccggcaac cccaggtcgc acaagatcac atcggggatg
     1741 cagtcgagcg ccgcacgcat tgcatcggct ccggtatgca caacctgaac ctggaaaccg
     1801 gaaagctcga gcagttccgc aaagttgtcc gcgacatcca cgttatcctc gaccaggagg
     1861 aggacctgac cgccaccgcg ctgtctctgg gatgcggtcc gcacggcagt gtgaccctcg
     1921 ttcaactcag gcgcgagcgg aatcctgagc gtaaaaatgg cgccctgaga gggcccgtcg
     1981 ctctcccctc tcagagaacc tccttgcagt cttgccaggc ggctcgcgat ggcaagcccg
     2041 agtcccaggc cccctccagg gcgacccggg ccatgttcct cttgtgtgaa gggttcgaag
     2101 agttcgctag caaactgctc atcgaagccc acgcctgtgt cccgcacggt gatgcatgcg
     2161 agactgccct cccgctggag tctcacccgg atggcacccc cggccggggt aaacttgatg
     2221 gcgttcgaga gtagattatc gatgatctgc cgcaggcgga cctgatccgc cttaacgcat
     2281 gaaggcggct tctcctcgat gctgacctcc agcatcaagc cagcttcgcg cactcgcgac
     2341 tgttgctcct ccaacacttc ttgcaaaagg ttcactaggt cgagggtttt gagctccacc
     2401 tcgactttac cgagagcaat ccgcgaggca tcgagaagat catcgagcat gcgcgccatc
     2461 gccgtgctct gccggtcgag ccgctggagc aatcgcaccg agcgctcatc ggcactgcct
     2521 acgagtttca ttgcatcggc ggcactgcga atcgcggcaa gtgggtttcg caattcgtga
     2581 gccagaaccg caatgaactg atccttctgc cgatccttct cctcgagcgc ttgcttggct
     2641 ctctcgagct cctgccgctc gtgcagctcg cgcagcgcgc tcagctcctc gtaaagccgc
     2701 tcacccatct cgcagcgatc ggcttcggga tcttcgagca gcagcttggc gaggtcctca
     2761 tgcgctccca gcagcgaaag catgtcctcc tcggactcga tctccgggtg gagcatcgtc
     2821 cagtaatgcg cagtgcggat gaaggcgagg cagccgcaaa agtactcgaa gttgcgggct
     2881 cccagggcgt gcagcaatgc gccgcgcgct cgcgccgaac gcgcaggctc gacgaacatc
     2941 acggccgcgc acgcaaaaaa
//
LOCUS       DATDMP010000088         6963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1569867, whole genome shotgun sequence.
ACCESSION   DATDMP010000088 DATDMP010000000
VERSION     DATDMP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569867"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VI195_01460"
     CDS             <1..1066
                     /locus_tag="VI195_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY6823078.1"
                     /translation="VRINTGFANERWFLPPGMESRVGADLLAAVRGNNGASVNYVLVR
                     STDGLPILAAAHLESLVGYAAALLRDPRVGDVSSPVNLQQGLGLDDYRHLYADLPQAL
                     KDYPEVATHFLSHDGRSAIFEVTPAERIAAGDIVRLSRDLQTRAPGGPFSVMVAGGPA
                     TYSDFRDAMSRSLPRIFAFVVGVTLLMLFAAFRSLLLPVKAVLMNLLAVAAGMGTVVA
                     VFQFGWLNGLIGLEHPVSTIPLEIPLMVFCLSFGLSMDYELFLLFRIQQEYYRDADNN
                     RATVEGLATVAPVITGAGLIMVVVFGAFVSAALPVLKMIGLGLCVAVLVDATLIRALI
                     VPAVMALAGRWNWYPGRRPA"
     gene            1146..1925
                     /gene="amrB"
                     /locus_tag="VI195_01465"
     CDS             1146..1925
                     /gene="amrB"
                     /locus_tag="VI195_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY6823079.1"
                     /translation="MAGMFYPDSPAVLGAQVRSYLKQVACRAQPAERPKALIVPHAGY
                     VYSGPIAAAAYARLAGACADIRRVVLLGPAHRVMLRGLAVPSADSFATPLGEIEVDRA
                     AVSSALELPQVSESDRAHALEHSLEVQLPFLQTLLSNFRLVPFAVGDTTAAQVAEVIE
                     LLWGGAETLIVISSDLSHYHAYREARAIDEASARTVLALSPTLDYEQACGALPINGLI
                     SVAHRRGMRPELLDLRNSGDTAGDKFRVVGYASFVFNEQRS"
     gene            1922..2494
                     /gene="amrA"
                     /locus_tag="VI195_01470"
     CDS             1922..2494
                     /gene="amrA"
                     /locus_tag="VI195_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEY6823080.1"
                     /translation="MTDLDLGRAVLTLARSAIAEKLALGTLEAANHPRLEQSCASFVT
                     LRKGAELRGCVGSLQPVRTLREDVQLNAIAAAFRDPRFPPLGASELANTSIEVSLLSP
                     GERIEAASEMELLAQLRPGVDGVTLEWRAHRATLLPQVWQHIGDPRDFLAALKVKAGL
                     PEEFWSAEVLVSRYAVTTWKEPGSTAPPGD"
     gene            complement(2501..3430)
                     /gene="amrS"
                     /locus_tag="VI195_01475"
     CDS             complement(2501..3430)
                     /gene="amrS"
                     /locus_tag="VI195_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY6823081.1"
                     /translation="MVPVRSIGRVPYEGEVYNLEVDDPDHSYLAPFAAVSNCQNWDMS
                     KSREMDTLADSASPAHLAAAADRLGCASVAFTYNDPVVFMEYAIDTAQACREMGIRSV
                     AVTAGYMCAPPRVELYRHMDAANVDLKGFTERFYYKVCGGELGAVLETLEYLQHETNV
                     WFEITTLLIPGENDSDSEIDQMTQWVAEKLGADVPLHFTAFHPDWKMLDKPPTPPATL
                     TRARRIALANGLHFVYTGNVHDREGGSTWCAGCGTCLIERDWYQLGNWKLTDSGACRN
                     CGVQLPGVFAGAPGNWGARRVGVRLAPRGRGGG"
     gene            3434..3583
                     /locus_tag="VI195_01480"
     CDS             3434..3583
                     /locus_tag="VI195_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823082.1"
                     /translation="MIASVSPERSFGGIGSVNARPIASLALWHDEIMIAGELRRLDQS
                     NIVDI"
     gene            4309..4773
                     /locus_tag="VI195_01485"
     CDS             4309..4773
                     /locus_tag="VI195_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY6823083.1"
                     /translation="MAVISAGLLLYRRGDPGLEVFLVHPGGPYWARKDEGAWSVPKGL
                     VDAGEEELACARREFQEETGFAAPSGGAELDLGTFRQPSGKLVHVWAIEGDCDPAQLN
                     SNLFEMEWPPKSGRTAQFPEADRGGWFDRAQALAKIVNGQRPILERFYAGLR"
     gene            complement(4755..5276)
                     /locus_tag="VI195_01490"
     CDS             complement(4755..5276)
                     /locus_tag="VI195_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEY6823084.1"
                     /translation="MSSLGSRSFRVISGGQTGVDRGALDAALDLGVSCGGWCPDGRRA
                     EDGIIPARYPLTELDGAGYDERTRRNVADSDDTLIITFGPASGGTARTIAACRELGRP
                     HLIIDGSTVAPEHAAGEAVSFVREKGIARLNVAGPRARGEPRGHGYCYALVRVLCLQC
                     AGSGANADQRKPA"
     gene            complement(5315..5749)
                     /gene="trxC"
                     /locus_tag="VI195_01495"
     CDS             complement(5315..5749)
                     /gene="trxC"
                     /locus_tag="VI195_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEY6823085.1"
                     /translation="MSDALHLVCPHCEKINRIAAGRLGERPKCGHCHQPLFTGHPLEL
                     TTAGFRRQIEHSDVPILVDFWAPWCGPCQMMAPQYAAAAAELEPGMRLAKVNTDAETE
                     LASEFQIRSIPTLTLFRGGREVARQPGAMARADIVRWVRQHA"
     gene            complement(5799..6548)
                     /locus_tag="VI195_01500"
     CDS             complement(5799..6548)
                     /locus_tag="VI195_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823086.1"
                     /translation="MNEASRPAGQTSVLIVGVGAWEGLGAALARRFARGGHPVVIAGR
                     NGEKLEATAALLKKTAAAAAFVIGDAAQAADVQRFVAQAERLAPLGVAIHNAGSNEPG
                     PFLQVSEQRFTQHWREHALGGFQLAQAALPLLLQHGGGSLFFTGASASLRGKAMFAPF
                     ASAKAALRNLAQSIAREYGPQNIHVGHVVVDGGIGGERLLSRNPQLRERRGADGLLDP
                     DAIAEAFWNLHHQQRSAWTLELDLRPWSESF"
     gene            complement(6720..>6963)
                     /locus_tag="VI195_01505"
     CDS             complement(6720..>6963)
                     /locus_tag="VI195_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEY6823087.1"
                     /translation="EGVEVGRGESSAPFNVPAHGESDFDMVVTTNLAMSVPNLAMRLF
                     RGGALPTYRLSGSVNPDIALLPPIPFWKSGQIALPN"
BASE COUNT         1180 a         2285 c         2248 g         1250 t
ORIGIN      
        1 ggtacgcatc aataccggct tcgccaatga acgctggttc ctgccgccgg gaatggagtc
       61 gcgcgtgggt gcggatctgc tcgccgcggt acgcggcaat aacggcgcgt ccgtgaacta
      121 cgtgctcgtg cgcagcaccg atggactgcc gatcctcgcc gcagcgcacc tcgagtccct
      181 ggtcggctat gccgcagcgc tgctgcgtga tccgcgcgtc ggcgatgtga gctcaccggt
      241 caatctgcag caagggctcg gcctcgatga ttaccgccac ctctacgccg acctgccgca
      301 agcgctcaag gactacccgg aagttgcgac tcacttcctg agccacgatg ggcggtccgc
      361 aatctttgag gtgacgccgg ccgagcgcat cgctgccggc gacatcgtgc gcctgtcgcg
      421 cgacctgcag acccgtgcgc ccggcggacc cttcagcgtg atggtggccg gcggcccggc
      481 gacctacagc gacttcaggg acgccatgtc acgcagcctg ccgcgcatct tcgcgttcgt
      541 cgtcggcgtg acgctgctga tgctgttcgc cgccttccgc tccctgctgt tgccggtcaa
      601 ggcggtgctc atgaacctgc tcgccgtggc ggccgggatg ggtaccgtgg tcgcggtgtt
      661 tcagtttggc tggctcaacg gactgatcgg actcgagcac ccggtctcga ccataccgtt
      721 ggagattccg ctcatggtgt tctgcctgag cttcggactg tcgatggact acgagctgtt
      781 cctgctgttc cgcatccaac aggagtacta ccgcgacgcg gacaataacc gcgccacggt
      841 cgagggtctc gccaccgtcg ctccggtgat cacaggcgcg ggcctcatca tggtggtggt
      901 cttcggggcc ttcgtgagtg cggcattgcc ggtgctgaaa atgatcggac ttggattgtg
      961 cgttgcagtg ctcgtcgatg cgacgctgat ccgcgcattg atcgtgcccg cggtgatggc
     1021 ccttgccggg cgctggaact ggtacccggg aagacggccg gcctagaagc tcaaccactc
     1081 cgcagttgat ttcccgggcc gcttggctca taccgggagc cgtggccgcc gtacgccctg
     1141 ccgcagtggc cggaatgttc tacccggatt cgcccgcagt tctgggtgca caggttcgta
     1201 gctacctcaa gcaggtggcg tgccgcgcgc aaccggcgga gcgaccgaaa gcgctcatcg
     1261 ttccacacgc cggctatgtt tactccggcc ccatcgccgc tgccgcgtat gcccggctgg
     1321 ctggcgcgtg cgcagacatc cggcgcgtcg tactgctggg ccctgctcac cgcgtgatgc
     1381 tgcgcgggct tgcggtcccc tcggcagact ctttcgccac gccgctcggc gagatcgagg
     1441 tggaccgcgc cgcggtgagt tcagcgctgg agttgccgca agtcagtgaa agcgaccgcg
     1501 cgcatgcgct cgagcactcg ctcgaggtgc aactgccctt cctgcagacg ctgctgtcca
     1561 actttcgtct cgtgccgttt gcggtgggcg atacgaccgc tgcgcaggtg gccgaggtca
     1621 tcgaattgct gtggggcgga gcggaaacgc tcatcgtcat cagctccgac ctgtcccact
     1681 accacgccta ccgcgaagca cgcgcgatcg atgaggcgag cgcacggacc gtgctggcac
     1741 tgtcgccgac gcttgactac gagcaagcct gcggagcctt gcccatcaat ggcctcatca
     1801 gcgtcgcgca ccggcgcggc atgcggccgg aactgctcga tctgcgaaac tcgggcgata
     1861 ccgctggcga caaattccgt gtggtcggct acgcatcgtt cgtcttcaac gagcagcgct
     1921 cgtgacggat cttgacctcg gccgcgccgt gctcacactc gcacgttcgg cgatcgccga
     1981 aaagctcgcg ctgggcacgc tcgaggcggc gaaccacccg aggctcgagc aatcctgtgc
     2041 atcattcgta accctgagaa agggggctga gctgcgcggc tgcgtcggct cactgcagcc
     2101 ggtgcgcacg ctgcgtgagg acgtgcagct caatgcgatc gccgctgcct tccgcgaccc
     2161 gcgctttcca ccgctcggcg caagcgagct cgccaacacc tcgatcgaag tgtcattgct
     2221 gtcgcccgga gagcggatcg aagcggcctc cgaaatggag ctgctggcgc agcttcgtcc
     2281 tggagtggat ggtgtgacgc tcgagtggcg agcgcaccgc gcgacactcc tgccacaggt
     2341 ctggcagcac ataggcgatc cacgcgattt ccttgcagca ctgaaggtca aggcggggct
     2401 gccggaggaa ttctggagcg cagaggtgct cgtcagccgc tatgcggtca cgacgtggaa
     2461 agagcccggt agcaccgcac cgccaggcga ctgacccccg tcagcctcct ccgcgcccgc
     2521 gcggagcgag ccgcacaccg acgcggcgtg cgccccagtt gccgggtgca cccgcgaaga
     2581 ctccaggaag ctgcacgccg cagttccggc aggcgccgga gtccgtcagc ttccagttcc
     2641 cgagttgata ccagtcacgt tcgatcagac acgtgccgca gccggcacac caggtgctac
     2701 caccttcgcg gtcgtgtacg ttgccggtgt agacaaagtg cagaccgttt gcgagcgcga
     2761 tacggcgtgc acgcgtaagc gttgccggcg gtgtcggtgg cttgtcgagc atcttccagt
     2821 cggggtggaa cgccgtgaag tgcagcggca cgtccgcgcc gagtttctcg gcgacccatt
     2881 gcgtcatctg atcgatttct gagtccgaat cgttctcgcc cggaatcagc agggtcgtga
     2941 tctcgaacca cacgttggtt tcgtgctgca ggtactcgag cgtctcgagg actgcgccga
     3001 gctcgccgcc gcacaccttg taatagaagc gctcggtgaa ccccttgaga tcgacgttgg
     3061 ccgcgtccat gtggcggtac aactcgactc gcggtggcgc gcacatgtag ccggccgtca
     3121 cggcgacgct tctgataccc atctcgcggc aggcctgcgc cgtgtcgatc gcgtactcca
     3181 tgaacacgac ggggtcgttg taggtgaacg cgacacttgc gcacccgagc ctgtcagcgg
     3241 cggcggcgag atgcgcgggg ctcgcactgt cggcgagcgt gtccatctcg cgcgatttcg
     3301 acatgtccca attttggcaa tttgagaccg cagcgaaggg cgcaaggtag gaatgatcgg
     3361 gatcgtccac ctcgaggttg tagacctccc cttcgtacgg aacccgtccg atgcttcgca
     3421 ccggcaccat gaaatgatcg ccagcgtctc gccagagcgc tcgttcggag gcatcggcag
     3481 cgtcaatgcg cgccccatcg ccagcttggc gctttggcac gacgagataa tgatcgctgg
     3541 agagcttcgc cgcctcgacc aatcgaacat cgtcgatatt tgatcgatgc gcggcaaaga
     3601 ctctgtgatt gggcgtcagc aacatcgctg ggcaacaggc agccttgagt gcgagcacct
     3661 cgcccgcata cccgtgcaca aacaccttgg aaacccgtgc cggcttaccc gcgcgtgtcc
     3721 agacggcaag ggagcccggg agcccaatct ttccctcgtc cctgaaaatt ttctcctcgc
     3781 agctgtcgaa tagatcggca ataggtcgca tcccatcagt ggtggcgatg agagtgctcg
     3841 ggacgaagca aaacttacac gccagattgc atcccgccgt cccgaaggac aggacgctcg
     3901 tgcccgggag aaaatgattg agcggcttct tctcgatcgg atcgatgcaa aatccgctcg
     3961 accggccata gctgaacagc ttgatccggc cttcctccgc accgcgcacg aagcacaggc
     4021 cgcgttggcc atcgcgaagc ttgcaggctc gcggacacac atcgcattgc acgcgtccat
     4081 cgtccagcgt gtgccaatag cgggtctcga caaccgactg ctgcggcggt ctcggcgcga
     4141 taacctcggg ctcgttcatg agctcattat gcgccgtcgc gcccaagcag cgctgagccg
     4201 gcgggcggct ctgggcgcac gcgcattacc ctgcacagga gccaggcgca tactctttcc
     4261 ttaaagaagg agcacgggag cagagacggt gccgtaccgg cagtgaccat ggcggtgatc
     4321 agcgcgggct tgttgcttta ccgtcggggc gacccgggcc tggaggtgtt cctcgttcat
     4381 cctggaggac cctattgggc caggaaggat gaaggcgctt ggtcggtgcc caaggggctg
     4441 gtcgatgcgg gcgaggagga gctcgcgtgt gcgcggcgcg agttccagga agagaccgga
     4501 ttcgccgcgc catcgggcgg cgcggagctg gaccttggaa cgtttcggca gccgagcggc
     4561 aagctcgtgc acgtgtgggc gatcgaaggc gactgcgacc cagcacagct caacagcaat
     4621 ctcttcgaga tggagtggcc gcccaagtcc ggtcgcacgg cacagttccc ggaggcggac
     4681 cgcggtgggt ggttcgatcg agcacaggcg ctcgccaaga tcgtgaacgg ccagcggccg
     4741 attctcgaac gcttctatgc cggcttgcgc tgatcggcgt tggcacccga accggcacac
     4801 tgcagacaga gcacgcgcac cagtgcatag caatagccat ggccgcgcgg ttcgcctctc
     4861 gcccgcgggc ctgcgacgtt cagccgcgcg atgcctttct ctcgcacgaa gctcaccgcc
     4921 tcaccggctg cgtgctctgg tgcgaccgtg gaaccatcga tgatgaggtg cgggcgtccg
     4981 agctcccggc aggccgctat ggtccgcgcg gtcccgccgc tggcagggcc gaaggtgatg
     5041 atgagcgtgt catcgctgtc cgcgacgttc ctgcgcgtgc gttcatcgta gcccgcgccg
     5101 tcgagctcgg tcagcgggta acgtgcgggt atgattccat cttcggcgcg ccgaccgtcg
     5161 ggacaccagc caccacagga cacccccagg tcgagcgcgg catcgagtgc gccacgatca
     5221 accccggttt gacctcccga gatcacacga aacgagcgtg agccgagcga gctcatgacc
     5281 tgaccgattc cgcgctaagc tgacatcaag cctttcaggc gtgttgacgc acccagcgca
     5341 cgatgtcggc tcttgccatg gcgccgggct gccgggcaac ctcgcggcca cctcggaaca
     5401 gagtcagagt tggaatgctg cgaatctgga attcgctcgc gagctccgtt tccgcgtcgg
     5461 tattgacctt ggcgaggcgc atgcccggct cgagctccgc ggccgccgcg gcgtactgcg
     5521 gcgccatcat ctggcacgga ccgcaccacg gagcccagaa atccacgagg atgggtacat
     5581 cactgtgctc gatctgccgc cggaaacccg cggtggtgag ctcgagcggg tggcccgtga
     5641 acagcggctg atggcagtgt ccacacttgg ggcgctcgcc gaggcgcccc gccgcaatac
     5701 ggttgatttt ctcgcagtgc gggcagacga gatgcaatgc gtcgctcatg cccgcggtat
     5761 ggggagctgc tggggagttt tcaagccgca cggtccgctc agaagctctc gctccacggc
     5821 cgcaggtcga gctccagtgt ccaggcgcta cgttgctgat gatgcaggtt ccagaacgct
     5881 tcggcgatcg catccggatc gagcaggccg tccgcgccgc gtcgttcccg cagctgcgga
     5941 ttgcgcgaca gcaaccgctc accgccaatg ccgccatcga cgaccacgtg accgacgtgg
     6001 atgttctgcg gcccgtattc gcgcgcgatg ctctgtgcga ggttgcgcag tgccgccttg
     6061 gcggatgcaa acggtgcgaa catcgccttg ccacgcaggc tggcagaggc cccggtgaag
     6121 aagagcgagc ctccaccgtg ctgcagcagc aagggcagcg cggcctgtgc cagctgaaat
     6181 ccaccgagcg cgtgctcacg ccagtgctgc gtgaagcgtt gctcgctcac ctgcagaaaa
     6241 ggccccggct cgttgctgcc ggcgttgtga atcgccacgc cgagtggagc gagacgctcg
     6301 gcctgcgcca cgaagcgttg tacatccgcg gcttgtgccg catcgccgat gacaaacgcg
     6361 gcggcggcag cggttttctt cagtagtgcc gccgtagctt cgagcttctc gccgttgcgg
     6421 ccggcaatga caaccgggtg tccgcctctt gcgaagcggc gggccagggc tgctcccagt
     6481 ccctcccagg cccccacgcc aacgatgagt acgctcgtct gtccggcggg tctcgatgcc
     6541 tcgttcatcg catcactgcc tcaatggcca cggatgtggt ggtcggatag gcagcaaggt
     6601 aggagcgctc cccgaaggtt caagaggcga cccgtcggtc atcaggtgca cgccaggaat
     6661 ttacggctca gctcacgccc cggtcaggcg ctgtcgccgc agtggtcttt gccggcgatt
     6721 cagttgggca gcgcgatctg acccgacttc cagaacggga tcggcggcag gagcgcgata
     6781 tccggattca cactccccga gaggcgataa gtcggcagcg cgccgccccg gaacagtcgc
     6841 atggcgagat tcgggacgct catcgcaaga ttcgtggtga cgaccatgtc gaaatcgctc
     6901 tcaccgtgag cgggcacgtt gaaaggcgca ctgctctcgc cacggccgac ctcgaccccc
     6961 tcg
//
LOCUS       DATDMP010000089         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_792954, whole genome shotgun sequence.
ACCESSION   DATDMP010000089 DATDMP010000000
VERSION     DATDMP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792954"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VI195_01510"
     CDS             complement(<1..1127)
                     /locus_tag="VI195_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY6823088.1"
                     /translation="MLFNATQQEELRVAIVDGQKLVDLDIESASREQRKSNIYKGLIT
                     RIEPSLEAAFIDYGEERHGFLPFKEVSRAYFKPDIDVGRARIQDALREGQEVIVQVEK
                     DERGTKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDTLDQLQLPEGM
                     SVIARTAAIGRSFEELNWDLNYLLQLWSAVESASSDQSGKPFLIYLESSLVIRAIRDY
                     FQPDIGEILIDTEEIYEQARAFMQTVMPGNVGKVKLYRDDVPLFSRFQIEHQIESAYS
                     RQVALPSGGAIVIDHTEALVAIDVNSARSTRGGDIEETAYRTNLEAADEVARQLRLRD
                     LGGLIVIDFIDMESMKNQREVENRLREALHYDRARVQTGKIS"
     gene            1421..2359
                     /locus_tag="VI195_01515"
     CDS             1421..2359
                     /locus_tag="VI195_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY6823089.1"
                     /translation="MNDLSKAPAKGAAYWIEVGDEAATQRIDNFLLRRLKGVPKSHVY
                     RVLRSGEVRVNSGRVKPEYRLQAGDRIRVPPMRVPEKPAPARPAEFPVVYEDPALLVV
                     DKPSGVAVHGGSGVSQGVIESLRASRPQAKVLELVHRLDRDTSGLLMIAKKRSALVEL
                     HRMLREGEVDKRYTVVVKGEWPADLVELRESLHKYVTASGERRVSVQADGLSAFTKVK
                     VMKKNAGFSVLEARLLTGRTHQIRVHLAHAGHPVLGDDKYGDFALNRTLGREGVKRLF
                     LHASRLRLRHPLTGDRLALEAPLPEDMDAFLRKKAA"
     gene            2356..3003
                     /locus_tag="VI195_01520"
     CDS             2356..3003
                     /locus_tag="VI195_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY6823090.1"
                     /translation="MSYALVVFDWDGTLIDSASTIAECIQEAARDIGLEPPSREQATH
                     VIGLGLHDSLRIAVPQLRPEQYVEFVSLYRKHFHAREDRMRLFPGIEPLLKSLSKERA
                     LAIATGKSRRGLDRALDATGLRPYFRGSRCADETHPKPHPAMLLELMEECAVDKRSTL
                     MIGDTSHDLEMARDAGVDALAVTYGAHPEPALRACGPLGCVGDVEGLQRWLSANA"
BASE COUNT          501 a          970 c         1041 g          539 t
ORIGIN      
        1 gagatcttgc cggtctgcac gcgggcgcgg tcgtaatgca gcgcttcgcg caggcggttc
       61 tcgacctccc gctggttctt catcgactcc atgtcgatga agtcgatgac gatcaggccg
      121 ccgaggtcgc gcagcctgag ctggcgcgcc acttcgtcgg ccgcctccag gttggtgcga
      181 taggcggtct cctcgatgtc gcctccgcgc gtcgagcgcg cggagttgac gtcgatggcg
      241 acgagcgcct cggtgtggtc gatcacgatg gcgccgcccg agggcagggc cacctggcgc
      301 gaatacgcgc tctcgatctg gtgctcgatc tggaagcgcg agaagagcgg cacgtcgtcg
      361 cggtagagct tcaccttgcc gacgttgccg ggcatcacgg tctgcatgaa ggcccgcgcc
      421 tgctcgtaga tttcctcggt gtcgatgagg atctcgccga tgtccggctg gaagtagtcg
      481 cggatcgcgc ggatgacgag gctcgattcg agatagatga ggaacggctt cccggactgg
      541 tcgctggaag ccgactcgac ggcactccag agctggagca ggtagttcag gtcccagttg
      601 agctcctcga aggagcgccc gatcgccgcc gtgcgcgcga tcacgctcat gccttccggg
      661 agctgcagct ggtcgagggt atcgcgcagc tcgttgcgat cctcgccttc gacccggcgc
      721 gagacgccgc cgccgcgggg gttgttcggc atgagcacga ggtagcggcc ggccaggctg
      781 atgaacgtgg tgagggcggc gcccttggtg ccgcgctcgt ctttctccac ctggacgatg
      841 acttcctgcc cctcgcgcag cgcgtcctgg atgcgcgcgc ggccgacgtc gatgtcgggc
      901 ttgaaatagg ccctcgaaac ttccttgaat ggaaggaagc cgtgccgctc ctcgccgtag
      961 tcgatgaagg cggcctcgag gctgggctcg atgcgggtga tcagcccctt atagatgttg
     1021 ctcttgcgtt gttcccttga ggcagattcg atgtcgaggt cgacgagctt ctggccgtcg
     1081 acgatcgcga cgcgcagttc ttcctgctgc gtcgcattga acagcatgcg cttcacttgt
     1141 ttttctcctg cgcgcgcagg agcaccgagc tcgtgggtcc gtgctctccg attggttttt
     1201 gatcaaaggg aattgcagct ggggaaaagc cgggctgcgc gcgcttctaa tggcttctag
     1261 tagtatcgct acttccggtg agcgaaatta accagcgctt caggccaggc tttcagccgg
     1321 gcaggaggcg ggttgcaggt ctgcgcttac ggggcttttg gatagaaccc cttgaagacc
     1381 tgtgcctcgc aactcgaagc gaccagagat catacggaaa atgaatgact taagcaaggc
     1441 gccggcgaaa ggcgcggctt actggatcga agtgggcgac gaggcggcga cccagcgcat
     1501 cgataacttc ctcctgcgcc gcctgaaggg cgtaccgaag agccacgtct accgggtcct
     1561 gcgcagcggc gaagtccggg tcaacagcgg ccgggtgaag cccgaatacc ggctccaggc
     1621 cggggaccgg atccgggttc cgccaatgcg ggtgccggaa aagccggcgc cagcgcgccc
     1681 ggccgaattc ccggtggtct acgaggatcc ggcgctgctc gtcgtggaca agccttctgg
     1741 cgtcgcggta cacggcggca gcggagtgag ccagggcgtg atcgagtcgc tgcgcgcatc
     1801 gcgtcctcag gccaaggtgc tcgagctcgt ccatcggctg gaccgcgaca cctccggcct
     1861 cctcatgatc gccaagaagc gcagcgctct cgtcgagctc caccggatgc tgcgcgaagg
     1921 cgaggtggac aagcgatata ccgtggtcgt gaaaggcgaa tggccggcgg atcttgtgga
     1981 gctgcgcgag tcgctccata aatacgtgac cgcgagcggg gagcggcggg tgagcgtcca
     2041 ggcggacggc ctgagcgcct tcaccaaggt caaggtcatg aagaagaatg ccggcttcag
     2101 cgtgctggaa gcgcgcctcc tcaccggccg tacgcaccag atccgggtgc acctggccca
     2161 cgccggacac cccgtgcttg gcgatgacaa gtacggcgac tttgcgctca accgcaccct
     2221 cggccgcgag ggggtgaagc gcctcttcct gcacgcgagc cgcctgcggc tcaggcatcc
     2281 gctgaccgga gatcggctcg cgctcgaggc gccgctgcct gaggacatgg acgctttcct
     2341 gcggaaaaag gcggcatgag ctacgcgctt gtcgtattcg attgggacgg caccctcatc
     2401 gactccgcga gcacgatcgc cgaatgcatc caggaggccg cgcgcgatat cggcctcgag
     2461 ccgccaagcc gtgagcaggc cactcacgtg atcggcctcg ggctgcacga ttccctgcgg
     2521 atcgccgtgc cgcagctgcg tcccgagcaa tacgtcgaat tcgtttcgct ctatcgcaag
     2581 catttccacg cgcgcgagga ccgcatgcgg cttttcccgg gcatcgagcc gctgctgaag
     2641 agcttgtcga aggagcgtgc cctggcgatc gcgacgggca agagccggcg cggcctggac
     2701 cgcgcgctcg acgcaaccgg cctgcgcccg tatttccgcg gctcgcgatg cgccgacgag
     2761 acccacccga agccgcatcc cgcgatgctg ctcgagctca tggaagagtg cgccgtcgac
     2821 aagcgctcca cgctcatgat cggggacacc tcgcacgacc tggaaatggc gcgcgacgcg
     2881 ggcgtcgacg ccctggcggt gacctacggc gcgcatcccg agccggccct gcgggcctgc
     2941 ggtccgctcg gatgcgtggg ggatgtggag ggcctgcagc gatggctgag cgcgaacgcc
     3001 taatctgcgc ctcgggggcg ctcgcggatt ccgggcctgg cgtgcgcttc g
//
LOCUS       DATDMP010000090         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1792579, whole genome shotgun sequence.
ACCESSION   DATDMP010000090 DATDMP010000000
VERSION     DATDMP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792579"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VI195_01525"
     CDS             <1..1095
                     /locus_tag="VI195_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6823091.1"
                     /translation="GPTGATADLASRFLPLVFGSQQSQVARVLAQQHGLSTASAMELL
                     RMAVFLLLTCFGSRQAGSSLDAGALALMLHAEAPVLRSYLPGALLGSAAGVARSRTPG
                     PVAPPRWLVPAAIAGALLLGWLVIRSFSASPETPAVATVTTEATNGAAAPPGNGTREA
                     MTAAWAALGDMMKVKLPDGSVLNVPELGVEARLVRFLSDSAPASDSSWFDFDRLLFNT
                     GKATLHGASLEQLANVAAILKAYPQVRIRIGGYTDNTGDAAANLRLSEERADNVMDEL
                     VKLGIDPARMSARGYGAQNPVADNSTEEGRQKNRRISLRVTDKQPSAGALNAVVRPVA
                     GRTGSVSRSTIGPALPPAADPVRRQSAAPP"
     gene            complement(977..1633)
                     /locus_tag="VI195_01530"
     CDS             complement(977..1633)
                     /locus_tag="VI195_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823092.1"
                     /translation="MHDRPRVPVSALALVLFLSMAPGARAQAPAAETVPPTWAVWTPK
                     ELRFVYMGFTSHYSCDGLRDKMRSILLQLGARPDLKVREVPCSGFAGRPTEFPGVTVN
                     MNVLAPWDTAQANASATPVPAHWKTVEISTDRDPLREAGDCELIEQVKSRVLPLFNAQ
                     RIEYHSSCIPNQLQIGGTQLKAELLIADEQGPPPAAAPDQSSNGSPTPSARPPGAPPR
                     "
     gene            complement(1745..>2590)
                     /locus_tag="VI195_01535"
     CDS             complement(1745..>2590)
                     /locus_tag="VI195_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY6823093.1"
                     /translation="SAVQVLREGGCGGVVPATRALRHEWALDAVAQQWAEGTPLAAAA
                     ERGGYAALATAGLHISASDRAVLEALRRSACRTLTGPELRALGVYRRGIDSWIVMTSD
                     YRTVSIPAAPSHTAPTIPWTPAPPAVESPRAPAPHNPPAASLASEALLLVNDVRSRGT
                     RCGERTFAPAPPLALSGSLGNVAYGHATDMAEHDYFEHRDLNGQSPADRVRAVGYREK
                     VVGENIAYGPKSVEEVVQGWLDSPGHCENIMDPRFAEMGIAYASGRSGRHGLYWVQVL
                     AEPQA"
BASE COUNT          421 a          876 c          892 g          401 t
ORIGIN      
        1 ggaccgaccg gcgcgacggc cgatctggcg agccgtttcc tgccgctggt gttcggctcg
       61 cagcagagtc aggtagcccg cgtactcgcg cagcagcacg gcctgagtac ggcatctgca
      121 atggaactgc tgaggatggc cgtgttcctg ctgctgacat gcttcggcag ccgacaggct
      181 ggcagcagcc tcgatgcggg tgccctcgcc ctcatgctgc acgccgaggc acccgtgctg
      241 cgcagctacc tgccgggcgc tctgctgggc agtgccgcgg gagtcgcgcg cagccgcacg
      301 ccggggcctg tcgcgccgcc gcgctggctg gtaccggccg ccatcgccgg tgcgctgctg
      361 ctcggctggc tcgtgatccg ctcgttctcc gcttcgccgg agaccccggc ggtcgcgacc
      421 gtaaccacgg aagcgaccaa cggtgcggcg gcgccgccgg gcaacggcac aagggaagcc
      481 atgacggcgg cctgggcggc cctcggcgac atgatgaagg tgaagctgcc ggatggcagt
      541 gtgctcaacg tgccggagct cggcgtggaa gcgcgcctgg tgaggttcct gagcgacagc
      601 gcgcctgcca gcgacagcag ctggttcgat ttcgaccgcc tgcttttcaa caccggcaag
      661 gcgacgctgc acggtgcctc actcgagcag ctcgccaacg ttgccgccat cctcaaggcc
      721 tacccgcaag tgagaatccg catcggcggc tataccgaca ataccggcga cgccgcggcg
      781 aatttgcggc tctccgagga acgcgccgac aacgtgatgg atgagctcgt gaaactcggt
      841 atcgatccgg cgcgcatgag cgcccgcggt tatggcgcgc agaacccggt agcggacaac
      901 tccaccgaag agggccggca gaagaaccga cgcatctcgc tgcgcgtgac cgacaagcag
      961 ccctcagcgg gagccctcaa cgcggtggtg cgcccggtgg ccgggcggac ggggtcggtg
     1021 agccgttcga cgattggtcc ggcgctgccg ccggcggcgg accctgttcg tcggcaatca
     1081 gcagctccgc cttgagctgc gtgccgccaa tctgcagctg gttgggaata cagctcgagt
     1141 gatactcgat gcgctgcgcg ttgaagagcg gcagcacacg ggacttcacc tgctcgatca
     1201 gctcgcagtc tccggcttcc cgcagcggat cgcggtcggt gctgatctcc accgttttcc
     1261 agtgcgccgg caccggggtc gcgctggcgt tcgcctgtgc agtgtcccac ggcgcgagca
     1321 cgttcatgtt cacggtcacg cccggaaact cggtgggcct tcccgcgaac ccggagcacg
     1381 gcacttcacg caccttgaga tcgggacgcg cgccgagctg cagcaggatc gagcgcatct
     1441 tgtcccgcag accgtcacag gagtaatggg atgtgaagcc catgtagacg aaacgcagct
     1501 ccttcggcgt ccataccgcc caggtgggcg gaacggtctc cgccgccgga gcttgcgctc
     1561 gcgccccggg ggccatcgag aggaacagga cgagtgccag tgcactcacg ggaacgcgcg
     1621 gtcggtcgtg catgatcctt cttccaggta acaacaccat attctcggct attgagacaa
     1681 catcggcagc cgggtgtttt ccggcggaac gggagcgcag gacgagggag ccttcaacgc
     1741 ggcctcaagc ctgcggctcg gcgaggacct gcacccagta cagaccatgc cgacccgacc
     1801 ggcccgacgc gtaagcgatg cccatctcgg cgaacctcgg gtccatgatg ttctcgcagt
     1861 gcccggggct atcgagccag ccctgcacga cctcttccac ggactttggt ccgtaggcga
     1921 tgttctcgcc gaccaccttc tcgcggtacc cgaccgcccg cacacgatcg gccggcgatt
     1981 gtccattgag gtcgcgatgc tcgaagtagt cgtgctcggc catgtcggtt gcgtgtccgt
     2041 aagcgacgtt gccgagcgag cccgaaagcg caagcggcgg agcgggagcg aatgtgcgct
     2101 cgccgcagcg tgttccgcgc gagcgcacgt cgttcacgag cagcagcgcc tcactcgcga
     2161 gggatgccgc gggtgggttg tgtggtgctg gagcgcgagg actctcgacc gccggggggg
     2221 cgggagtcca gggaatcgtg ggagcggtgt ggctcggggc ggcgggaatg gaaacagtgc
     2281 gatagtccga agtcatcacg atccagctat cgatcccgcg gcggtacacc ccgagcgcgc
     2341 gcaactccgg cccggtgagc gtgcggcagg cggaacggcg caacgcttcg agcactgcgc
     2401 gatcggaagc gctgatgtgc agcccggcgg tcgcgagcgc agcgtagccg ccgcgctccg
     2461 ctgccgcggc gagcggcgtg ccctctgccc actgctgtgc gaccgcatcc agcgcccact
     2521 cgtgacgcag cgcgcgggtg gccggcacga cgccgccgca accgccttcg cgcagcacct
     2581 gcaccgccga
//
LOCUS       DATDMP010000091         7027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_793257, whole genome shotgun sequence.
ACCESSION   DATDMP010000091 DATDMP010000000
VERSION     DATDMP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793257"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1568
                     /locus_tag="VI195_01540"
     CDS             36..1568
                     /locus_tag="VI195_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6823094.1"
                     /translation="MSDNNDGAFNRRDFVRGSMSAAALAALPAAMLAAAADAEADKAV
                     VLAQIPKMHADNIRRLQEWIALPSIAAENRNYPQGAEHMAQLARAAGFTGVKLVPTSG
                     KPGVFGRLDAGARTTVGIYFMYDVKQFVPEEWSSPPLEGRLVQKAGLGTVCMGRGAVN
                     QKGPENSFLSALMAFKAAGRKLPVNLVLVCEGEEEIASPHFSEIVMNPQVLPELKKCM
                     GVWMPEAAQDRDGGVQVNLGAKGVVELELVSSGEKWGRGPKHDIHSSLEAAVDSPSWH
                     LVQGLNTLIEKDGHTPAVEGFYDKVRPLTAQQKKMIEDYAAKTSEETVKKTLAVDRWV
                     HDAGWVQSRMLLASRPTINIEGLVGGYTGPGGKTVLPHRAVAKIDMRLVPDMTATDTL
                     AKLKAHLAKHGFADIEVNMSGGYDPTQTDPDSRLIQTQLTTYRRLGSDPLLWPRLGGS
                     WPGCVFTGAPLSLPAGHFGMGHGTGAHAPDEYYLIESTNPKVQGLDGAVASFVEYLYA
                     LA"
     gene            1622..1695
                     /locus_tag="VI195_01545"
     tRNA            1622..1695
                     /locus_tag="VI195_01545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1654..1656,aa:Gly,seq:ccc)
     gene            2100..3398
                     /locus_tag="VI195_01550"
     CDS             2100..3398
                     /locus_tag="VI195_01550"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823095.1"
                     /translation="MHQIGVVGLSYRHAAVDEVARFAVPRAEVPARLPELRALLKAAE
                     ILYVGTCNRVEVLYSTEDGTPAADLRQQVFRSLTGREPAPGEAARVLRAWTGEAAVEH
                     LLLVACGLDSAQTGEHEIATQLRGAWEDSRAAHACGPILDRLMGEALGMARRMRRLSA
                     GLHTPSLADLAADRVLQHLGGARESVALLGVSPMTRRCAAALHGARVPLVIVNRTLAA
                     AEELAATVSGQALALEVFRLRPPAVSALVLAAGGGTALLDAAALLRLKAAAPRAPLLV
                     DFGVPPNVDPEAARRAGLGRLGMHELIELARGRRLAQLVRLAPVRAAIDERLTRLRTE
                     LAVRAIGPRLARLRETFEQMAAAEVARALKGELRTLDARERAQLERLAATLAHRLAHL
                     PLAGLRAAAVHGGSDAVEAFFDGAHAPRRPALDAADTGET"
     gene            3395..4717
                     /gene="hemL"
                     /locus_tag="VI195_01555"
     CDS             3395..4717
                     /gene="hemL"
                     /locus_tag="VI195_01555"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY6823096.1"
                     /translation="MSEERTTLEPPTVRPRSAELYARACRVIPGGVNSPVRAFRAVGG
                     TPVFIARGSGAQIFDADGRAYLDFVGSWGPLIAGHAHPAVVAAVTAALGRGMTYGAPC
                     AAEVELAEQVVAAYPAAEQVRLVSSGTEAVMSAIRVARAFTQRELIVKFAGCYHGHAD
                     HLLVAAGSGLATFGRPSSAGVPEAFVGCTRVLPLDDEAAAAELFAREGARIAAVIIEP
                     VPANHGLLPQRRVFLQALRALTQQHGALLIFDEVISGFRLGRGGAAERLGIAPDLATF
                     GKVIGGGMPVGAFGGGRRIMARLAPDGDTYQAGTLSGNPVAMAAGVATLELLARESGW
                     ERLEARGAELERLLVPVLSAARFPVQLVRVGSLFWLSFHAAGAPRVAAPLTTAESARF
                     AALFHAMLDRGIYLPPSAYEACFLSLAHGPAELTRLAEALRESLAMIA"
     gene            4714..5823
                     /locus_tag="VI195_01560"
     CDS             4714..5823
                     /locus_tag="VI195_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6823097.1"
                     /translation="MKATRVFQLTALALVVVSAVQVGWWLFDQHAYTVEKVSAARAAY
                     TQQVAAAQALLDAGATTQRVQQLLPGMVVRGGHASLAPELTSALTTEEHRRYRQYAWE
                     GSFFLLALAACIAVIARALRAEAHVREQQDSFLALVSHQFKTPLASLQLSLETLALRA
                     PGPEAARPLIERMLADLARMEQMVTQILESARLERGRVELKPEPLQLAGAVQRVIAQL
                     EERARAEHVSITTDIAPAFLVVSDPLALDVVVRNVLENALAAVKAAGGGRITLSAHAT
                     EREVELQVADSGVGFAAADGARLFHKFSRLRGSAHYGTGLGLYIVRRLMEMADGRVSA
                     QSDGLGQGARVRLTWPAAAAAAVPQPAPGREATRS"
     gene            5820..6545
                     /locus_tag="VI195_01565"
     CDS             5820..6545
                     /locus_tag="VI195_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6823098.1"
                     /translation="MSAPPAAQVLVVEDDPHLAAGVVENLRAEGYEVSIAADGEQALA
                     WLAQHSCALVILDVMLPGADGLSVCRTLRARGDTTPVLFLTARGDPADRVRGLEAGGD
                     DYLAKPFHLQEFLLRVRAILRRWDWYRSASATSATAMLHFGGNEVDFRAFRARAWNGE
                     AQELTEKEAMILKVLVEHTGQIVSREDLLERVWGYDVFPSTRTVDNFILRLRKRFERD
                     PANPRHFLTVWGVGYRFLVEGEP"
     gene            6542..>7027
                     /gene="hemC"
                     /locus_tag="VI195_01570"
     CDS             6542..>7027
                     /gene="hemC"
                     /locus_tag="VI195_01570"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY6823099.1"
                     /translation="MSGSRTLRIGTRASALALWQAREVATRIAAQPGAPGIELVHIRT
                     SGDLQSDVPLWQTRGRAFFTREIDRALLAGEVDIAVHSLKDLPTQLETGLTLAALLPR
                     ADPRDALVSRSGALLAQLPARARIGTSSLRRRAFLARLRPDAVLLELRGNVPTRIERL
                     QR"
BASE COUNT         1030 a         2406 c         2449 g         1142 t
ORIGIN      
        1 ggtgcgggct tccgccacgc gcgtggaggg gatccatgtc tgacaacaat gacggcgcgt
       61 tcaaccggcg tgatttcgtg cgtggctcga tgtcggcagc ggcgctcgcc gctttgcccg
      121 cagcaatgct cgctgcggca gcggatgccg aggcggacaa ggcggtggtg ctggcgcaga
      181 ttccgaagat gcacgccgac aacatccgcc ggctgcagga gtggattgcg cttccctcga
      241 tcgccgcgga gaaccgcaat tatccgcagg gagcggagca catggcgcag cttgcccggg
      301 cggcgggctt caccggcgtg aagctcgtgc cgacctccgg caagcccgga gtcttcgggc
      361 gtctcgatgc cggcgcgcgc acgaccgtcg gcatctactt catgtatgac gtgaagcagt
      421 tcgtgccgga ggagtggagc tcgccgccgc tcgaggggcg cctggtgcag aaggccggtc
      481 tcgggaccgt gtgcatgggg cgcggcgccg tcaaccagaa aggacccgag aactcgttcc
      541 tgagcgcgct catggccttc aaagccgcag gacgcaagct ccccgtgaac ctcgtgctgg
      601 tctgcgaggg tgaggaggag atcgcctcgc cgcacttctc cgagatcgtc atgaatcctc
      661 aggtgcttcc cgagctcaag aaatgcatgg gcgtgtggat gcccgaggcc gcacaggacc
      721 gtgacggcgg ggtacaggtc aacctcggcg ccaagggagt cgtggagctc gagctcgtct
      781 cgagcgggga gaagtggggg cgcggcccga agcatgacat ccactcgagc ctcgaggcgg
      841 cggtcgactc cccctcctgg cacctggtgc agggcttgaa cactctcatc gagaaggacg
      901 gccacacccc ggcggtcgag ggcttctacg acaaggtgcg gccacttacc gcgcagcaga
      961 agaagatgat cgaggactac gccgccaaga cctccgagga gacggtgaaa aagacgctcg
     1021 ctgtcgatcg gtgggtgcac gatgccggtt gggtgcagtc gcgaatgctg ctcgcctccc
     1081 gcccgaccat caacatcgaa gggctggtgg gcggctacac cggtcccggc ggcaagacgg
     1141 tcctgccaca ccgtgcggtg gccaagatcg acatgcgcct cgtgcccgat atgactgcga
     1201 cggacacgct cgccaagctg aaggcgcatc tggcgaagca cggcttcgcc gacatcgaag
     1261 tcaacatgag tggcggctac gaccccacac agaccgaccc cgacagccgc ctgatccaga
     1321 cacagctcac gacctaccgg cggctcggct ccgatccact gttgtggccg cgcctgggcg
     1381 gatcgtggcc ggggtgcgtg ttcaccggcg caccgctcag cctgccggcc gggcatttcg
     1441 gcatggggca cggcaccggc gcgcatgcgc cggatgagta ctacctcatc gagtcgacca
     1501 atcccaaggt gcagggactc gatggtgccg tggcatcatt cgtcgagtat ctgtacgcac
     1561 tcgcctgaga cgcggtgccg cgttgccgaa ccgagggacg cgggctaaag taacaatctg
     1621 cgcgggtgtc gtacaatggt agtactgcag cttcccaagc tgctaacgtg ggttcgattc
     1681 ccatcacccg ctccattcac cgctgcgcgc gccgttcccg gaagatgagg cgtttgcgcc
     1741 gtaccatgcc taggggaaat tcctgggctc cctcatgatc agcagcaggc agagccgtcg
     1801 ccggtcggca gcaggtgggc tgctgttgtg gctcgcgcag cttttcgtcc cggcgtccgc
     1861 tgcagtgggt ctcacccaat ccgatacgct catgctcgca ggcttcgaga gccgaagcgc
     1921 aacaatccca cagggcctgc gcgaggcact tccggcaacc tcgcacaggc ccgcgcgctc
     1981 agcgagcgag ccgtgggctg aaggccgcta gcggctgatg tggggtttgt gcggcgcgtc
     2041 tccggcgccg tgaccattcg atgacatacc ccttccgccg aggcggttaa gcttgcggga
     2101 tgcaccagat cggtgtcgta ggcctctcct atcgccacgc ggctgtggat gaggtcgcgc
     2161 gcttcgccgt gccacgggcg gaagtaccgg cgcggctgcc ggagttgcgc gccctgctca
     2221 aggccgcgga aatcctctat gtcggcacct gcaaccgcgt cgaggtgttg tattcgaccg
     2281 aagacggcac gcccgccgcg gacctgcgcc agcaggtctt ccgcagcctc actggccgcg
     2341 agcccgcgcc cggcgaagct gcgcgggtgc tgcgtgcctg gaccggtgag gcggcggtag
     2401 agcatctgct gctggtcgcg tgcggattgg attccgcgca gaccggcgag cacgagatcg
     2461 ctacgcagct gcgcggcgcc tgggaggact ctcgcgccgc acacgcctgc ggtccgattc
     2521 tcgatcgcct catgggcgaa gcgctcggca tggcgcggcg catgcggcgg ctctcggccg
     2581 gcctgcacac accctcgctg gcggacctcg ccgccgaccg cgtcctgcaa catctcggtg
     2641 gggcgcgaga gagcgtggca ctgctcgggg tgtcgcccat gacgcgccgg tgcgcggccg
     2701 cgctgcacgg cgcgcgcgta ccactcgtca tcgtcaaccg cacgttggca gccgcggagg
     2761 agttggccgc aaccgtctcg ggccaggcgc tcgcgctcga agttttccgt ctgcggccgc
     2821 cagcggtgag cgctctggtg ctggccgcgg gtggcggcac ggcgctgctc gatgcggcgg
     2881 cgctcttacg gctcaaggcc gccgcgccac gcgcaccgct gctggtcgac ttcggcgtgc
     2941 cgccgaatgt cgatcctgag gcggcgcgtc gcgcgggtct cggccggtta gggatgcacg
     3001 agctcatcga gctggcccgc gggcggcggc tcgctcagct cgtgcgtctc gcgccggtgc
     3061 gcgcggcgat cgatgagcgg ctgacgagat tgcgtaccga gctggcagtc cgcgcgatcg
     3121 gtccccgtct cgcgcgcttg cgcgagacgt tcgagcagat ggccgcggcg gaggtggcac
     3181 gcgcgctcaa gggagagttg cgtacactcg acgcccgcga gcgtgcgcag ctcgagcggc
     3241 tcgccgcaac cctggcgcac cgcctcgcgc acctgccgct cgccggactg cgcgccgcag
     3301 cggtgcatgg cggcagcgat gcggtggagg cattcttcga tggggcacat gcgccacggc
     3361 gccccgctct cgacgccgct gatacggggg agacatgagc gaggagcgca ccaccctcga
     3421 gccgcccact gtgcggccgc gctcggccga gctgtacgcg cgcgcgtgcc gggtcattcc
     3481 cggaggggtc aactcgcccg tgcgcgcctt ccgcgccgta ggcggcacgc cggttttcat
     3541 cgcgcgtggg tccggcgcgc aaatcttcga tgccgatggc cgcgcttatc tcgacttcgt
     3601 cggctcatgg ggtccactca ttgccgggca cgcgcatccc gcagtcgttg ccgcggttac
     3661 cgcggcgctc ggacgcggca tgacctacgg cgcgccgtgt gcggcggaag tggagctcgc
     3721 cgaacaggtg gtggccgctt accccgcggc cgagcaggtg cgcctggtgt catccggcac
     3781 cgaggcggtg atgagcgcga tccgtgtggc acgcgccttc actcagcgcg agctcatcgt
     3841 caagtttgcc ggctgctatc acggacatgc cgatcatctg ctggtggcgg ccggctcggg
     3901 actcgccact ttcggccggc cttcctcggc tggtgtgccc gaggctttcg tcggctgcac
     3961 gcgcgtgctg ccgctcgatg acgaggcggc agccgcggag ctcttcgccc gcgagggcgc
     4021 acgcatcgcc gcagtgatca tcgagcccgt gcccgccaat cacggtctgc tgccgcagcg
     4081 gcgcgtcttc cttcaggcgc tgcgcgcgct cacgcagcag cacggtgcgc tgctgatctt
     4141 cgatgaggtg atctcgggtt tccgcctcgg ccgcggcggc gccgccgagc ggctcggtat
     4201 cgcacccgac cttgcgacct tcggcaaggt gatcggtggc ggcatgccgg tcggggcgtt
     4261 cggcggcgga cgccgcatca tggcgcgcct cgcgcccgat ggggacacct atcaggccgg
     4321 cacgctctcg ggcaacccgg tcgccatggc ggcgggcgtc gcgacgctcg agctgctcgc
     4381 acgcgagagc ggctgggagc gcctcgaggc tcgtggcgct gagctcgagc gactgctcgt
     4441 gccggtactg tccgcggcac gcttcccggt gcagctggtg cgggtcggct cgctcttttg
     4501 gctctcattt cacgccgcgg gcgcaccgcg cgtggcggcg cccctcacta cagcagagtc
     4561 ggcccgtttt gccgcgctgt tccacgccat gctcgatcgc ggcatctatc tgccgccctc
     4621 agcttatgag gcgtgcttcc tgtcactcgc tcacggtcct gccgagctca cgcgcctggc
     4681 cgaagcgctg cgtgagtcgc tggcgatgat cgcatgaagg ctacgcgcgt ctttcagctc
     4741 accgcgctcg cgctcgtggt ggtgtccgcg gtgcaggtgg gctggtggct cttcgaccag
     4801 cacgcttaca ccgtcgagaa ggtctcggcc gcgcgtgcgg cctacacgca gcaggtggcc
     4861 gccgcgcagg cgttgctcga tgcgggcgct acgacgcagc gggtgcagca gctgcttccc
     4921 ggcatggtgg tgaggggtgg acatgcctca ctcgctcccg agctcaccag cgcgctcacc
     4981 accgaagagc accgccgcta ccggcagtac gcctgggaag gctccttctt cctgctcgcg
     5041 ctcgcagcct gcatcgcggt aatcgcgcgt gcgttgcgcg ccgaggcgca cgtgcgcgag
     5101 cagcaggaca gctttctggc cctcgtctcg caccagttca agacaccgct cgcgagtctg
     5161 cagctgtcac tggagacact ggcgttgcgc gcgccgggcc cggaggccgc gcggccactc
     5221 atcgagcgca tgctcgccga tctggcccgc atggagcaga tggtcacaca aatcctcgag
     5281 agcgcgcgtc tcgagcgtgg gcgggtggag ctgaagcccg aaccgctgca gctggccggc
     5341 gcggtgcaac gcgtcatcgc gcagctcgaa gagcgcgcgc gcgcggagca cgtcagcatc
     5401 acgaccgaca tcgctccggc attcctggtc gtgagcgatc cgctcgcact cgatgtggta
     5461 gtgcgcaatg tgctcgagaa tgccctcgcc gccgtgaagg ccgcgggcgg cggccgcatc
     5521 acgctcagcg cccatgcgac cgagcgggag gtggagttac aggtagccga cagcggagtc
     5581 ggcttcgcgg ccgctgacgg cgcgcgctta ttccacaagt tcagccgctt gcgtggcagc
     5641 gcccactacg gcaccggcct cggcctctac atcgtacggc ggttgatgga gatggccgac
     5701 ggccgtgtca gcgcgcagag cgatggcctc ggacagggcg cccgcgtgcg gctcacctgg
     5761 cccgccgctg ccgccgccgc cgttccgcaa ccggcacccg ggcgggaggc gacgcggtca
     5821 tgagcgcacc gccggcggcg caggtgctcg tggtcgagga cgatccacac ctcgcggccg
     5881 gtgtggtgga aaatctgcgc gccgaaggct acgaggtgag tatcgctgcg gacggggagc
     5941 aggcgctggc gtggctcgca caacacagct gcgcactcgt catcctcgat gtgatgttgc
     6001 cgggagccga tgggctcagc gtatgccgca cgctgcgtgc gcgcggcgac accaccccgg
     6061 tgctgttcct cacggcgcgc ggcgatccgg ccgatcgcgt gcgtgggctc gaggcggggg
     6121 gtgatgacta tctggcgaag ccgttccacc tgcaggagtt cctgctgcgc gtgcgtgcca
     6181 tcctgcgacg ctgggactgg tatcgcagcg cctcagccac gagcgccacc gccatgctgc
     6241 atttcggcgg caacgaagtg gacttccgcg cgttccgcgc ccgcgcctgg aacggcgagg
     6301 cccaggagct caccgaaaag gaagcgatga ttctcaaagt gctggtcgag cacaccggac
     6361 agatcgtctc gcgcgaggat ctgctcgagc gcgtctgggg ctacgacgta ttcccctcca
     6421 cgcgtaccgt ggacaacttc atcctgcgcc tgcgcaaacg cttcgagaga gaccccgcca
     6481 acccgcggca tttcctcacg gtgtgggggg tgggctatcg ctttctcgtc gaaggtgagc
     6541 catgagcggc agccgcactc tgcggatcgg cacccgtgcc tccgcgctcg ctctgtggca
     6601 ggcacgcgag gtggccacgc gcatcgctgc gcaacccggt gcacccggca tcgagctggt
     6661 gcacatacgc acctccggcg acctgcagag cgatgtgccg ctgtggcaga cacgcggccg
     6721 cgcatttttc acccgtgaaa tcgatcgcgc gctgcttgcc ggcgaggtcg atatcgccgt
     6781 gcacagcctc aaggacctgc ccactcagct ggagacgggg ctcacgctcg cggcactgct
     6841 gccgcgcgcg gatccgcggg acgccctcgt gagccgcagc ggcgcactcc tggcgcagct
     6901 tccggcgcgc gcacgcatcg gcacgagcag cctgcggcgt cgagcgttcc tcgcgcgtct
     6961 gcgcccggat gcggtgctgc tcgaactgcg cggcaacgtg ccgacgcgca tcgagcggct
     7021 gcagcgt
//
LOCUS       DATDMP010000092         2818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1125958, whole genome shotgun sequence.
ACCESSION   DATDMP010000092 DATDMP010000000
VERSION     DATDMP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125958"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VI195_01575"
     CDS             complement(<1..858)
                     /locus_tag="VI195_01575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6823100.1"
                     /translation="MDYIRFGQSGLKVSQLCLGTMSMGSSAWKSWVLDERDSIPILRR
                     ALDLGITFFDMADWYSIGRNEEVVGRNLLRMTARERLVLATKVYYPMSDDPKDRGLSR
                     EHIAASIDRSLARIGTDYLDLYVIHAFDADTPVAETMEALHDTVRAGKVRHLGASTMY
                     AWQFAKMNHVAAMNGWTPFVNMQCQYNLLYREEEREMFPYCRDQGIAVTTFSPLARGF
                     LARAAATARTAHDVYQDYYGDEIDREIARRVHEVAARRGVSPAQVALAWVAGSPHLNV
                     PIVGASKTAH"
     gene            complement(868..1773)
                     /locus_tag="VI195_01580"
     CDS             complement(868..1773)
                     /locus_tag="VI195_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY6823101.1"
                     /translation="MTKLTAGAAPAGRALLLALVLSWLAAAPASAADPPGAPRPPGLA
                     SLPKVSSLRLYVIDCGTLVYNRPEDYNLTRAEVADTNMSVPCFLVIHPKGILLFDAGL
                     SDTLVGRPLYENVDGGYGQIKFTTLRGQLADIGVTPGDITYFAISHSHWDHVGNARDY
                     AGSIWLAQKAEIDFMFGPRADPAYVALYAALAHARQEVFDGDHDVFGDGTVILKLTPG
                     HTPGHQSLYVRLAATGGVLIAGDLYHYAEERTLHRMPREEESTGTARSREIIERFLKE
                     KNAQLWIGHSTAFFRNAIKSPGWYD"
     gene            2380..>2818
                     /locus_tag="VI195_01585"
     CDS             2380..>2818
                     /locus_tag="VI195_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823102.1"
                     /translation="MFAAGGALLQVSAQGNPPGQDREGSGAFDERIRINAAQALAAGR
                     NTFRFDTFGDEAFWGDSLQLQRAIEGSVLGGVGGGLSPKAALDLGLKVDASALPQAVI
                     NQLRQKAINLNDPAVTVALLKLNAVVGVQGFFNGNQLQSVGITC"
BASE COUNT          488 a          893 c          965 g          472 t
ORIGIN      
        1 gtgggcggtc ttcgaagcgc ccacgatcgg cacgttgagg tggggactgc ccgccaccca
       61 cgccagcgcg acctgtgccg ggctcacgcc ccggcgtgcg gccacctcgt gcacgcggcg
      121 cgcgatctcc cgatcgatct catcgccgta gtagtcctgg tagacgtcgt gggccgtgcg
      181 tgcggtcgcc gcagcgcggg cgaggaagcc gcgcgccagc ggactgaagg tcgtcacggc
      241 gataccctgg tcgcgacagt aggggaacat ctcccgctcc tcctcgcggt agagcaggtt
      301 gtactggcac tgcatgttga cgaagggtgt ccagccgttc atcgctgcca catggttcat
      361 tttggcgaat tgccaggcat acatcgtcga ggcgccgagg tggcgcacct tgccggcgcg
      421 gactgtgtcg tgcagcgcct ccatggtctc cgcgacgggc gtgtccgcat cgaaggcatg
      481 gatcacatag aggtcgagat agtcggtacc gatgcgcgcc agcgagcggt cgatcgaggc
      541 cgcgatgtgc tcgcgcgaca ggccacgatc cttcgggtcg tcgctcatcg ggtagtagac
      601 cttggtcgca agcaccagcc gctcgcgggc ggtcatgcgc agcagattgc ggccgacgac
      661 ctcctcgttg cggccgatgg aataccagtc agccatgtcg aagaaggtga tgccgaggtc
      721 gagggcgcgc cgcaggatgg gaatcgagtc ccgctcgtcg agcacccagc tcttccaggc
      781 ggagctgccc attgacatcg tgccgaggca aagctgcgac accttgaggc cgctctgccc
      841 gaagcggatg taatccatgc agggccctca gtcgtaccag ccgggtgact tgatcgcatt
      901 cctgaagaac gccgtgctgt ggccgatcca gagctgagcg ttcttttcct tcaggaaccg
      961 ctcgatgatc tcgcgcgatc gcgcagtgcc cgtgctctcc tcctcgcgcg gcatgcggtg
     1021 gagcgtgcgc tcctcggcgt agtggtacag atctcccgcg atcagcacgc cgcccgttgc
     1081 tgcgagcctc acatagagtg actggtgtcc cggggtgtgc ccgggagtga gcttgagaat
     1141 cacggtgccg tcgccgaata cgtcgtgatc gccgtcgaat acctcctggc gggcatgggc
     1201 gagcgcggcg tagagcgcga cataggcggg atccgccctg gggccgaaca tgaaatcgat
     1261 ctccgccttc tgcgccagcc agatcgagcc cgcgtaatcc ctggcgttgc cgacgtgatc
     1321 ccaatgcgag tgcgagatgg caaagtaggt gatgtccccg ggggtcacac cgatatcggc
     1381 gagctggccc cggagggtgg tgaacttgat ctgcccgtag ccaccgtcca cgttctcgta
     1441 cagcggccgg cccacgaggg tgtcggacaa cccggcatcg aacagcagaa tccccttcgg
     1501 gtgaatcacc aggaagcacg gcaccgacat attggtatcg gccacctcgg cgcgcgtgag
     1561 gttgtagtcc tcgggacggt tgtagacgag cgtgccgcaa tcgatcacgt agagccgcag
     1621 cgagctgact ttcgggagcg acgcgagccc cggcgggcgc ggcgcgcccg gtggatccgc
     1681 ggcactcgcc ggtgcggccg ccagccagct cagcacgaga gccaggagga gcgcgcggcc
     1741 agcaggagcg gcgccggcag tcaacttggt cacgcaggcc tggtagctca tgtgttaccg
     1801 ccgggacgtc tgggcgcaaa tggacagccg ctcgcgacgg cgcaagttgc cgactggcgc
     1861 gggcgctgaa ttattcacca gtgcggccgg ggccgaaacc cgtcggcggg ctctgcggcg
     1921 ctgattggct gcgggttgca gcttggatgt gcgcgcaaat ccttgaacgg cggggagtct
     1981 tgggtgcggc ggtggcatca cgtcggtagc cgcagccgac ggcgagcgcg gccgtggact
     2041 cgaccggcgc gcgacgcgtg agcgggagcc cagcgcgcca gcaccgcacc ggcgagccgc
     2101 cgttccggaa cgctcacgtg atcttccgga acgcgcagct aacattccca ccgatccgca
     2161 ccacggcctc cacgttatag tgcgccgtcg ttttcggtcg ccatcttaag gtggatttcg
     2221 ccgccaactt caccaagtca acaccacgcg agcagacggg cgcgatcggg tacctgaact
     2281 cagtccgcgc caaggaggag acgtatgccg gtagctacac gaaaatcagc cagcgggttt
     2341 ctcaagtggc gggggctggc agcggcggta ggcattgcga tgttcgcagc aggcggcgcg
     2401 ctcctgcagg tgagtgcgca ggggaatcct cccggccagg accgcgaggg atccggcgcg
     2461 ttcgatgagc gcatcaggat caacgcggcg caagcgctgg cggcgggccg taacaccttc
     2521 agattcgata cgttcggcga cgaggccttt tggggcgatt cgctgcaact tcagcgggcc
     2581 atcgaaggct cggtcctcgg cggcgttggc ggcggactca gtccgaaagc cgcgctcgac
     2641 ctggggctga aagtggacgc gagcgcactg ccgcaagcag tcatcaacca actgcggcag
     2701 aaagcgatca acctgaacga ccctgcggtc acggtggccc tgctcaagct caacgccgtg
     2761 gtgggtgtgc agggtttctt caatggcaat caactccagt cggtcggcat cacctgcg
//
LOCUS       DATDMP010000093         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1015241, whole genome shotgun sequence.
ACCESSION   DATDMP010000093 DATDMP010000000
VERSION     DATDMP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015241"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VI195_01590"
     CDS             <1..640
                     /locus_tag="VI195_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY6823103.1"
                     /translation="PGPENPLGKYAFYLQWPSYLSHGTNKPAGVGLRSSHGCIRLYPE
                     DIEQFFNMVPIGTEVRVVNQPFVFGWAEGELYMQAYDVMEDDTRDWKHAQKKLLTTSL
                     ATRLQQQIKAHHEQVDWSVVTSLARNPRGIPVSVTESNASLEQVLAAAPRVQNVLPEG
                     STWDGTSDLPMDEATFRQIVSEIEPGSAPAPSPAPVTPGASNAQRAAQKNGG"
     gene            843..2456
                     /locus_tag="VI195_01595"
     CDS             843..2456
                     /locus_tag="VI195_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823104.1"
                     /translation="MVDSRSAGAQLAQQRRDHHTIERVPTAVAAFIGRTLKGPVNQAL
                     TVHSFNEFQQYFGGLWQPSTVAYAVEQFFENGGREARIVRVANGARPPTLTLPAGRGA
                     LQLTALNPGSREYLRASVDYDGLTATEPERFNLVVQRVRTAGSELVEDQEIYRRLSVA
                     PASGRFVADVLLESRLVRVLGEMPAQRPERSASGPGGVAIGYTLSNPDGDDGAPLTDY
                     DIIGAAGSGTGLFALGGAAGFNLLCIPPLARDRDVGLSTLLVAARVCREHHALLLVDP
                     PAAWNSARAALEGLRTWPFRSDSALMYFPRVQAFDRLRGRVETFASCGAAAGMIARSD
                     EIWPVWAAAESEEAVLRPGLRPAVAVSEADRLRLAQAGVNTLLSVRTGPSNSISPRTL
                     AAGSGGASDWRYLSARRLALFVAASIEQGTRWVLLEHNGPSTWKRAQGLVESFLDELA
                     TQGAFAGAAAEESHFVIADERVNRPQTLAEGKFNLLFGFATSKPGEFDTWLVTHRAGA
                     SRVRPVSVNRSATSKQRVEWESETSILRG"
BASE COUNT          417 a          836 c          833 g          415 t
ORIGIN      
        1 accgggaccg gaaaacccgc tcggcaaata tgccttttac ctgcaatggc cgagctacct
       61 cagccacggc accaacaagc ccgccggcgt cggcttgcgc tcgagccatg gctgcatccg
      121 gctgtacccg gaggacatcg agcagttctt caacatggtg cccatcggca cagaggtgcg
      181 cgtcgtcaat cagccgttcg tcttcggctg ggcggagggc gagctgtaca tgcaggccta
      241 tgacgtgatg gaggacgaca cccgcgactg gaagcatgcg caaaagaagc tgctcaccac
      301 atcgctcgcc acacggctgc agcagcagat taaagcgcat cacgaacaag tcgactggag
      361 cgtggtcacg agcctcgcgc gcaatccgcg cgggattccg gtttccgtca cggagtcaaa
      421 cgcgagtctc gagcaggtgc tggccgcggc gccgcgggtg cagaacgtgc tgccggaagg
      481 ctcgacttgg gatggcactt cggatctgcc catggatgag gccacgttcc ggcagatcgt
      541 ttcggagatc gagccgggca gcgcaccggc accgagcccc gccccggtga cgccaggtgc
      601 ctcgaatgct caacgagccg cacagaaaaa cggcggctga cgcagcgcag ccgactgcgc
      661 tgcgggaaga gtcgcacggc tcggctttat cgccttggtt gcaactcatg ctcgcggaga
      721 tcagacgcaa gcacgaggat ctcgagcagg cgcgtgccga gcagcggcgc agggcggatg
      781 aggcgaacac gccctgacac gctgtgttag agtcgcgccg gcagattcac tgaggcgctg
      841 gagtggtcga cagccgtagc gcaggcgcac aactcgcaca acaacgtcgc gaccatcaca
      901 cgatcgagcg cgtgccgact gccgtcgccg ccttcatcgg ccgcaccctc aaaggacccg
      961 tcaatcaagc actgacggtc cacagtttca acgagttcca gcagtatttc ggtggcctgt
     1021 ggcaaccctc gacggtcgcg tacgcagtcg agcagttctt cgagaacggc ggccgtgagg
     1081 cgcgcatcgt gcgcgtcgcg aacggtgcgc ggccgccgac gctcacgctt cccgccggcc
     1141 gtggtgcgct gcagcttacg gcgctaaacc ccggctcgcg cgagtacctg cgtgcttcgg
     1201 tggactacga tggtctgact gcgaccgagc cggagcgttt caacctcgtc gtgcagcgcg
     1261 tgcgcaccgc agggtccgag ctggtcgagg accaggagat ctaccggcgc ttgtcggtcg
     1321 cacccgcctc cggccgtttc gtggcggacg tactgctcga atcgcgactc gtgcgcgtgc
     1381 ttggtgaaat gcccgcacag cgtccggagc gctctgccag cggccccggg ggcgtcgcga
     1441 tcggctacac cctctccaac ccggacggcg atgatggggc gccgctcacc gactacgaca
     1501 tcatcggcgc cgccggcagc ggcaccggac tgttcgcact cggcggtgcg gcgggcttca
     1561 acctgctgtg catcccgccg ctcgcacgcg atcgcgacgt cggcctctcg acgctgctcg
     1621 tcgcggcccg cgtatgccgt gagcatcacg ctctgcttct ggttgatccc ccagcggcct
     1681 ggaactcggc tcgcgctgca ctcgaggggc tgcgcacctg gccatttcgc agcgacagcg
     1741 ccctcatgta cttcccgcgg gtgcaggcat tcgaccggct gcgtggacgc gtcgagacct
     1801 tcgcctcctg tggtgccgct gccggcatga tcgcccgctc ggatgagatc tggccggtgt
     1861 gggcggccgc cgaaagcgag gaagccgtgc tgcgtcctgg cctgcggccc gcggtggcgg
     1921 tctcggaggc cgatcgcctg cgcctcgcgc aagctggcgt gaatacgctg ctctcggtac
     1981 gcaccggccc atcgaacagc atcagcccgc gcacgctcgc cgccggcagc ggtggagctt
     2041 cggattggag atacctgtca gcgcggcggc tggcgctgtt cgtggcagcg agcatcgagc
     2101 agggtacccg ctgggtgctg ctcgagcaca acgggccatc cacgtggaaa cgtgctcagg
     2161 ggctggtgga gagctttctc gatgagctcg ccacgcaggg cgcattcgcg ggagcagccg
     2221 cggaggagtc gcacttcgtc attgccgatg agcgcgtcaa tcggccccag actctcgccg
     2281 agggcaagtt caatctgctg ttcggcttcg ccaccagcaa gccgggtgaa ttcgacacct
     2341 ggctcgtaac ccaccgcgcc ggtgccagcc gcgtgcggcc ggtgtcggtg aaccgctctg
     2401 cgacttcgaa gcagcgcgtc gagtgggaga gcgagacttc gattctgcgc ggctagcttg
     2461 aggctgactc gcgcgtggcg ctcgcgcttg tatggtattc c
//
LOCUS       DATDMP010000094         2225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1015513, whole genome shotgun sequence.
ACCESSION   DATDMP010000094 DATDMP010000000
VERSION     DATDMP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015513"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VI195_01600"
     CDS             complement(<1..664)
                     /locus_tag="VI195_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6823105.1"
                     /translation="MYLEPFKLKELPFRLSPDPQFLYLSKQHARAKAYMESTIWFTDG
                     FVVITGEIGSGKTTLIESFLKEVPADVVVAQINQTQVTAIDFLQAVLVQFGFSPFKMR
                     KGELISTLNNFLIEQYAAGRKVLLIVDEAQNLSMRVLEEIRMLSGVETTKEKVLRIIL
                     AGQPELNSKLDDPGLEQLAQRVRLRFHLTTLSEAETRAYIEHRLEVAGAGDREVFAAD
                     TFH"
     gene            complement(687..1793)
                     /locus_tag="VI195_01605"
     CDS             complement(687..1793)
                     /locus_tag="VI195_01605"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY6823106.1"
                     /translation="MTVGIVGAGQLGRMLALAGYPLGLEFLCLDPSADAPAGQVAPLL
                     HGAFTDRRLLARLARQCEVITFDWENVSVSGLAGIARRHRVRICPPVAALQTGQDRVA
                     ETRLFERLRIPTTRWQAVDSRRELARAMAAVGVPGVLKTRRLGYDGKGQAVIRTAADA
                     ERGWAELGRVPLLYEEYVPFDCEVSIIGARSLRGETAVYPLCGNVHGAGVLRLTRAPY
                     GPARWQRRATRYLERVLAHFRYSGILTIEFFVRRGLLIANEMAPRVHNSGHWTIEGAT
                     TSQVENHLRAILGLPLGSTRARGFSAMVNLIGEQPPRRRLLALAGVHLHDYGKRARPG
                     RKMGHCTVVEPTAAARDARARALCKDLRVDVRIP"
     gene            complement(1790..>2225)
                     /gene="purE"
                     /locus_tag="VI195_01610"
     CDS             complement(1790..>2225)
                     /gene="purE"
                     /locus_tag="VI195_01610"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY6823107.1"
                     /translation="EMLDQLAIPYEVRVVSAHRTPDLLFEYAANSRARGLEVLIAGAG
                     GAAHLPGMTAAKTLLPVLGVPVQSKSLGGLDSLLSIVQMPAGVPVATFAIGTAGAANA
                     ALCAAAILANKHPPIASALEAFRERQTAGVLGNPDPRTPPRT"
BASE COUNT          371 a          744 c          765 g          345 t
ORIGIN      
        1 cgtggaaagt gtcggctgcg aacacctcgc ggtcaccggc cccggccacc tcgagccgat
       61 gctcgatgta ggcgcgcgtc tccgcctccg acagcgtggt gagatgaaag cgcagccgca
      121 cgcgctgcgc aagctgctcg agacccgggt catcgagctt gctgttgagc tcgggctggc
      181 cagcgagaat gatgcgcagc accttctcct tggtggtctc gacgcccgag agcatgcgga
      241 tctcttccag tacccgcatg gacaggttct gcgcctcatc gacgatcagc agcactttgc
      301 gcccggcggc gtactgctcg atgaggaaat tgttgagcgt gctgatcagc tcgcccttgc
      361 gcatcttgaa cggcgagaag ccgaactgca ccagcaccgc ctgcaggaaa tcgatcgccg
      421 tgacctgggt ctggttgatc tgcgccacca ccacatcggc cggcacctcc ttgaggaagg
      481 actcgatgag cgtggtcttg cccgagccga tctcgccggt gatcaccacg aacccgtccg
      541 tgaaccagat ggtcgactcc atgtacgcct tggcgcgggc gtgctgcttg gatagataca
      601 ggaactgcgg atccggactt aagcggaacg gcaattcctt gagtttgaag ggttcgaggt
      661 acatggctgt tggctcgcag ccgatgttac gggatgcgaa cgtcgaccct caaatcctta
      721 cacaaagccc gcgcgcgggc atcccgggcg gcggcggttg gctctaccac cgtgcaatgg
      781 cccatcttcc gcccggggcg ggcgcgcttg ccataatcat gtaggtggac tccggcaagc
      841 gccagcagcc gtcggcgcgg cggctgctcg cctatcagat tgaccatggc gctgaagccg
      901 cgcgcgcgcg tcgagccgag cggcagcccg aggatcgcac gcaggtgatt ctcgacctgg
      961 ctggtggtgg cgccctcgat ggtccagtgg ccggagttgt gcacgcgcgg cgccatctcg
     1021 ttggcgatca gcagcccgcg acgcacgaag aactctatgg tcagtatgcc gctgtagcgg
     1081 aagtgcgcga gcacacgctc gaggtagcgc gtggcgcgcc gctgccagcg cgcgggtccg
     1141 tagggggcgc gggtgagacg cagcacaccc gctccatgga cgttgccgca cagagggtag
     1201 accgcggtct cgccacgcag actgcgggcg ccgatgatcg acacctcgca gtcgaaaggc
     1261 acgtactcct cgtaaagcag tggcacgcgg ccgagctcgg cccagccgcg ctccgcatcc
     1321 gccgccgtgc gaatgacagc ctggcctttg ccgtcgtagc ccaggcggcg ggtcttgagt
     1381 acaccgggga ctccgaccgc ggccatggcg cgggcgagct cgcgccggga gtcgaccgcc
     1441 tgccagcgcg tcgttggaat ccgcagacgc tcgaacagcc gcgtctccgc aacgcggtcc
     1501 tgtccggtct gcaatgcggc gaccggcggg cagatgcgca cccggtgacg gcgtgcgatc
     1561 ccggcaagac cgctcaccga gacgttctcc cagtcgaagg tgatgacctc gcactgccgc
     1621 gcgagccgcg ccagcagccg cctgtcggtg aaagcgccgt gcagcagcgg cgccacctgg
     1681 ccggccggcg cgtcggccga ggggtccaga cacaggaact cgagtccgag cggataaccc
     1741 gccaaagcga gcatgcggcc gagctggccg gcgcccacga tgccgaccgt catgtgcgcg
     1801 ggggtgtgcg cggatcggga ttgccgagca cccccgcggt ctggcgctcg cggaacgcct
     1861 cgagagcgct cgcgatcggc gggtgcttgt tggcgaggat ggctgcggca cacagcgccg
     1921 cattggcggc acccgcggtg ccgatcgcga aagtcgcgac cggcaccccc gcgggcatct
     1981 gcacgatcga gagcagcgaa tcgaggccgc cgagtgattt cgactgtacc ggcacgccga
     2041 gcaccggcag tagcgtcttg gccgcggtca tgccgggaag gtgtgccgcg cccccggcgc
     2101 cggcgatcag cacctcgagc ccgcgggcgc ggctgttggc ggcatactcg aacagcaggt
     2161 ccggagtgcg gtgtgccgag acgacccgca cctcgtaggg gatcgcgagt tggtccaaca
     2221 tctcc
//
LOCUS       DATDMP010000095         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_349393, whole genome shotgun sequence.
ACCESSION   DATDMP010000095 DATDMP010000000
VERSION     DATDMP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349393"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VI195_01615"
     CDS             complement(<1..497)
                     /locus_tag="VI195_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823108.1"
                     /translation="MRICDDRYRRERARMELALRFLHHEARTQTIRAWTGLSDDRIRK
                     LYRSYMSHARRELPRHRGKSPHQVAYFTRSLRLQEESALLASVLSLLGVVPSAPSAGG
                     ARGLPLADVRRGELLCQAFEAFRLLLPAAQISFEHAVFLATALARGDQLRLGECSGCG
                     SLMVT"
     gene            630..1388
                     /locus_tag="VI195_01620"
     CDS             630..1388
                     /locus_tag="VI195_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823109.1"
                     /translation="MLLRCGKEESGTDEDATPGGCWLRASTLDSLTELNELGLALLAE
                     QSTVRSGVSSALLQPLAELWRALDGAGRRRAAACPYLLLDAGFGDAARWRTPPAAPQV
                     GDGAIDRYAGYFSVPAATELARLVFIFAWHLSRSQPAAARLLLGMPAASVTLVSRYTL
                     RQIEALAESHPEWLRPRWPTRVQVWRELLLAAAAGETVHLERARLRGLTLLAAEARLA
                     SARQAAADAGTLAPLAAHAGDHAGAPGRARLAGS"
     gene            complement(1454..>2075)
                     /locus_tag="VI195_01625"
     CDS             complement(1454..>2075)
                     /locus_tag="VI195_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY6823110.1"
                     /translation="ARAATWTEGVQYTRLTPAQQTTVAAGKVEVLEVFSYGCPACNAF
                     QPVMDKLRRALPANAQLAFLPAAFNPSEDWPMFQRAYFAAQALGIAERTHQAMYDAVW
                     KSGELGIVVPGGNRLKSPLPSIADAARFYARVAGIDAQQFLAMANSFGIDGKIRSADA
                     QILAMHVDSTPTLIVNGKYRVIRDELKNNDELIELVRYLVAKESGS"
BASE COUNT          315 a          689 c          755 g          316 t
ORIGIN      
        1 tcggtcacca tgagacttcc gcagccgctg cactcgccga ggcgcagctg atcgccgcgc
       61 gcgagcgccg tggcgaggaa cacggcatgc tcgaacgaga tctgcgccgc gggcagcagc
      121 agacggaagg cttcgaacgc ctggcacaac agctcgccgc gcctcacatc ggcgagcggc
      181 aggccgcgcg cgccgcccgc gctgggcgcg ctgggcacga cgccgagcag cgacagcacg
      241 ctcgcgagca gcgcgctctc ctcctgcagg cgcagcgagc gcgtgaagta ggccacctgg
      301 tgaggcgact tgccccgatg gcgcggaagc tcacgccgtg catgactcat gtaggaacga
      361 tagagcttgc ggatgcgatc gtcggagagt ccggtccagg cccgtatggt ctgggtgcgc
      421 gcctcgtgat gcaggaagcg cagcgcgagc tccatgcgcg cgcgctcgcg gcggtaacgg
      481 tcgtcgcaga tgcgcatgcc cggcattttg gtaaaaaatc accaaaaggc aatcggccaa
      541 aattggttaa aaaactccaa attcaccctg gcggctgcgc tgcgctcggc tacgatagtg
      601 gcgcgccgtc ggagcgcgga ggtggcgcga tgctgctgcg atgcgggaag gaagagtccg
      661 gcacggacga ggatgccacc ccaggcggct gttggttgcg cgcgagcaca ctcgattcgc
      721 tcaccgagct caatgagctc ggcctggcgc tgctcgcgga gcagtcgacg gtacgcagcg
      781 gcgtctcgag cgcgctgctg cagccgctcg cggagctgtg gcgcgcgctc gatggcgcag
      841 gccgccgccg cgcggcggcc tgcccgtacc tgctgctcga tgccggtttt ggcgatgcag
      901 cgcgctggcg cactccgccg gcggcgccgc aggtcggcga tggcgccatc gatcgctatg
      961 cgggctactt cagcgtgcct gcggccaccg agctcgcgcg cctggtcttc atctttgcgt
     1021 ggcatctcag ccgctcgcag ccggcggcgg cgcgactgct gctcggtatg ccggcggcga
     1081 gcgtgacgct ggtcagtcgc tacacgctgc ggcagatcga ggcgctcgcg gaaagtcacc
     1141 ccgagtggtt gcggccgcgc tggccgacgc gggtgcaggt gtggcgtgag ctgctgctgg
     1201 cggctgctgc cggagagacg gtgcatctcg agcgcgcgcg gctgcgcgga ttgacgctgc
     1261 tcgcggccga ggcgcgcctg gcatcggccc ggcaggccgc ggccgacgcc ggcaccctcg
     1321 cgccactcgc tgcgcacgcc ggcgatcacg cgggtgcgcc cgggagggca agactcgccg
     1381 gatcataaga ctgagcggcg gcgccggcgg tggctcgagc gccgcgttac gcggcccctc
     1441 gaggtgggtg ccttcaggac ccgctctcct tcgccaccag atacctcacg agctcgatga
     1501 gctcatcgtt gttcttgagc tcgtcgcgga tcacccggta cttgccgttc acgatgaggg
     1561 tgggcgtgga gtcgacgtgc atggcgagga tctgcgcatc cgcgctgcgg atcttgccgt
     1621 cgatgccgaa ggagttggcc atggcgagaa actgctgcgc atcgatcccg gcgacgcgtg
     1681 catagaagcg cgccgcatcc gcgatcgacg gcagcggact cttcaggcgg ttgccgccgg
     1741 gaacgacgat gccgagctcg ccgctcttcc acactgcgtc gtacatcgcc tggtgcgtgc
     1801 gctcggcgat gccgagggcc tgcgccgcaa aatacgcgcg ctggaacatc ggccagtcct
     1861 ccgaggggtt gaacgccgcg ggcagaaacg cgagctgcgc gttggcaggc agggcgcggc
     1921 gcagcttgtc catgacgggc tgaaaggcgt tgcaggcagg acagccgtag gagaacacct
     1981 ccagcacctc caccttgccc gcagctacgg tggtctgctg cgcgggtgtg aggcgcgtgt
     2041 actggacgcc ctcggtccag gtggcggcgc gcgcg
//
LOCUS       DATDMP010000096         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_571526, whole genome shotgun sequence.
ACCESSION   DATDMP010000096 DATDMP010000000
VERSION     DATDMP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571526"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VI195_01630"
     CDS             <1..865
                     /locus_tag="VI195_01630"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY6823111.1"
                     /translation="THEGMSADHIVTGTGSGELLCALALIYGRDGGEIIAARPTYDEL
                     PRYAQRSGAALKFVPVDAQLRHDLAAMHAAISARTRAVYLCNPNNPTGTVLPAAQIRD
                     FVRSLPAAVTTIIDEAYLDFVAEGATGSVADLVSAGQRVVVLRTFSKIHGMAGMRCGY
                     AIAPPEIATTLASARMTSPNLLAVRAARASLGDAPFLADCRQRILASRARITAELSNL
                     ELRYAEPQGNFVFFDTGMPLARFTTLMRERNILVGRLFPPFDTWCRITIGTEPEVTAF
                     LQALRALHTRA"
     gene            complement(873..1631)
                     /locus_tag="VI195_01635"
     CDS             complement(873..1631)
                     /locus_tag="VI195_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HEY6823112.1"
                     /translation="MLALVREVSPELARCELTHLERAPIDAARAAHQHRGYTEALRTL
                     GCTLEWLPPLPGFPDSVFVEDTAVVLDGIAIVTRPGASSRRGETPSVAAALERHRPAS
                     RILEPGCLDGGDVLHIGRTLYVGASGRTNAAGIAQLERLLQPHGYRVQATALAGCLHL
                     KSAGSFIPPDVLLVNPAWVDAAAFGKLRVVAVHEDEPYGANTLTIGATTLVSAAYPQT
                     RRRLEAAGVRTQTLDVGELHKAEGALTCMTLLLA"
     gene            1736..2341
                     /locus_tag="VI195_01640"
     CDS             1736..2341
                     /locus_tag="VI195_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY6823113.1"
                     /translation="MISVAHISGAELAVSIALGIGLAAAVGLRVFLPLLVMSIAASTG
                     HLTLGSGFAWLATPAALVMLSVAALLEVLAYYVPGLDNLLDAIATPAALVAGTVASAA
                     VMTDLPPIVKWTTAVIAGGGAAGLTQGFTSLLRAKSTVTTAGLGNHLIATGELAAALL
                     LSLLALAAPLLAAGVVALFCWSAVRMTRRLLRHDRAAGGSA"
     gene            2469..2621
                     /locus_tag="VI195_01645"
     CDS             2469..2621
                     /locus_tag="VI195_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823114.1"
                     /translation="MIRKLKSGQYRLYSRKLDPRTKKRRNLGTFDSRAAAEKHERAVQ
                     FFKRRG"
     gene            complement(2652..>2842)
                     /locus_tag="VI195_01650"
     CDS             complement(2652..>2842)
                     /locus_tag="VI195_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823115.1"
                     /translation="VSFVRVGTLDEPDRFPPDVHIYTESKQPWVLLPPGARAFSQYYK
                     SAEVWSAPSLARREALRH"
BASE COUNT          418 a         1010 c          938 g          476 t
ORIGIN      
        1 cacccatgag ggcatgagcg ccgatcacat cgtcaccgga accggctcgg gtgagctgct
       61 gtgcgcgctg gcactcattt atgggcggga cggcggcgag atcatcgccg cccggcccac
      121 ctacgatgag ctgccacgtt atgcgcagcg ctcgggcgcg gcgctcaagt tcgtccccgt
      181 cgatgcgcag ttgcgccacg acctcgccgc catgcacgcc gcgatctcag cgcgcacgcg
      241 cgcggtttac ctgtgcaatc ccaacaatcc cacgggcact gtcctgcccg ccgcgcagat
      301 tcgcgacttc gtacgctcac tgcctgcggc ggtgacgacc atcatcgatg aggcctacct
      361 ggacttcgtc gccgagggtg cgaccggctc ggtcgccgat ctcgtgagtg ccgggcagcg
      421 cgtggtggtg ttgcggacct tctccaagat ccatggcatg gccggcatgc gctgcggcta
      481 cgccatcgcg ccgccggaaa ttgccactac gctcgccagc gcccgcatga ccagtcccaa
      541 cctgcttgcg gtgcgtgccg cgcgcgcgag cctcggcgat gcgccgtttc tcgccgactg
      601 ccggcagcgc attctcgcga gccgcgcgcg catcaccgcc gagttgagca acctcgaact
      661 gcgctacgcc gagccgcagg gtaacttcgt cttcttcgat accggcatgc cgctggcacg
      721 cttcaccacg ctcatgcgcg agcgcaatat cctcgtgggc cggctgtttc cgccgtttga
      781 cacctggtgc cgtatcacca tcggcaccga gcccgaggtc acggcttttc tgcaggcact
      841 gcgcgcgctg cacacgcgcg cgtgaggcgc gctcaggcga gcagcagggt catgcaggta
      901 agtgcgccct ccgccttgtg cagctcgccc acatcgaggg tttgcgtccg tacccccgcc
      961 gcctcgagac gccggcgcgt ttgcgggtac gccgcactga cgagcgtcgt ggcgccgatg
     1021 gtgagggtat tggcgccgta gggctcgtcc tcgtgaaccg cgacgacacg cagcttgccg
     1081 aacgccgccg catcgaccca cgccggattc acgagcagca cgtccggtgg aatgaaactg
     1141 ccggcgctct tcagatgcag acagccagca agcgccgttg cctgcacacg gtagccgtgt
     1201 ggctgcagca gccgctcgag ctgtgcgatg cccgcggcgt tggtgcgccc ggaagctccg
     1261 acgtatagcg tgcggccgat gtgcagcaca tcgccgccat cgaggcagcc gggctcgagg
     1321 atgcgcgacg cgggtcgatg gcgttcgagc gccgccgcga cactcggcgt ctcgccgcgt
     1381 cgtgagctcg ctcctggccg ggttacgatg gcgatgccat cgagcaccac cgccgtatcc
     1441 tcgacgaaga cgctgtcggg aaatcccggc aatggcggca gccactcgag agtgcaaccg
     1501 agcgtccgca gggcctcggt gtagccgcga tgctggtgcg cggcgcgcgc ggcatcgatg
     1561 ggcgcgcgct cgaggtgcgt gagctcgcag cgcgcaagct cgggactcac ttctctgact
     1621 agcgcgagca tgacggcacc tgcatggcgc tggcgaggat agccgcagcg ccgctggcgt
     1681 gaaagccact ctggccggct cgacaggcgc gcaagccgcc cctatcgtaa cccgcgtgat
     1741 ttctgtcgct cacatctcgg gcgccgagct cgctgtctcg atcgccctcg gcatcggtct
     1801 ggccgcggcg gtcggtttgc gcgtgttcct gccgctgctg gtgatgagca ttgctgccag
     1861 caccggccac ctcaccctcg gcagcggctt cgcctggctc gcgacccctg cggcgctcgt
     1921 gatgttgagc gtggcagcgc tgctcgaggt gcttgcctac tacgtaccgg gcctggacaa
     1981 cctgctcgat gcgatcgcca cgccggccgc actcgttgcc ggcacggtgg cgtcggcggc
     2041 cgtcatgacc gacctgccgc cgatcgttaa gtggacgacg gcggtcattg ccggtggcgg
     2101 tgctgccggg ctcacgcagg gcttcacctc gctcctgcga gccaagtcca ctgtgacgac
     2161 cgccggactc ggcaaccacc tcattgccac cggcgagctc gcggccgcac tgctgctgtc
     2221 gctgctcgcg ctcgcagcgc cgctgctggc ggcgggtgtg gtggcactgt tctgctggtc
     2281 cgccgtgcgc atgacgcgac gcctgctgcg ccacgatcgc gccgcaggtg gcagcgcctg
     2341 aggcggacgc tcctcagccg ctgagacgtg gggacggcgc atcgcgcgcc gcgacgcggg
     2401 ttcgtctgac aggaggttcg tcaatcgact gacaccgcgc ggcaccgcct ccgcacgaga
     2461 gtcgcaccat gatccgtaag ctcaaatccg gccagtaccg cttgtattcg cgcaagctcg
     2521 atccgcgcac caagaaacgc cgcaatctcg gcactttcga cagccgcgcg gccgcggaga
     2581 agcacgagcg cgcggtgcag ttcttcaagc ggcgcggctg atccgtccag cgcgcgctgc
     2641 ctcagccgcg atcagtgccg caaggcctca cgccgcgcga ggctcggcgc gctccacacc
     2701 tcggcacttt tgtagtactg gctgaatgcg cgcgccccgg gtggcaacaa gacccatggt
     2761 tgcttcgact cggtgtagat gtgcacgtcg ggcgggaatc ggtccggctc atcgagcgtg
     2821 cccacgcgca cgaagctcac gc
//
LOCUS       DATDMP010000097         3840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_460473, whole genome shotgun sequence.
ACCESSION   DATDMP010000097 DATDMP010000000
VERSION     DATDMP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460473"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VI195_01655"
     CDS             <1..1106
                     /locus_tag="VI195_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6823116.1"
                     /translation="TALWAMACAAPLFVLAGAARHLILAPFGFDTVVEQLAAAFWFPR
                     VAGACIGAAVWAMLGFFNGIGRPRTTLLITLVTTITNAVLNQEFIFRLGWGIAGSAWA
                     TNVAQLAGLAFALTLFLAPGYRRRFGSHLTWRLQPARLMQQLRLGVPMGLSPAADLLG
                     FAIFQMMQTRLGTAGGAATQIVVILTSLAYMPGFGIASAGTTLVGQSIGAGARDWAQR
                     VGQRVILLTACYMGGIGVLIALGGPWVLPFFTGAHDADARPAILIGMQLLWLAAGYQF
                     FDGLNLGSSMCLRGAGDVRIPALLVLALSLLLFVPLAHALTFAPGQGWVRFLPQLGWG
                     AVGGWIAVLIYIMVLGSALFLRWRSRAWQTIQL"
     gene            1127..1906
                     /locus_tag="VI195_01660"
     CDS             1127..1906
                     /locus_tag="VI195_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY6823117.1"
                     /translation="MFIALKTLLHTLLLPPGGPLLLAAAGAWLIARGTGGRLRRAGWV
                     LLVAALATLWLLATPLVADALEAAAERCPALDLSRPVPAQAVVILGGAAGRLDAPEYG
                     GEPAAAWELLERLNYGAHIARRTGLPVAVSGSGDEVLAMNATLARDFGVATRWIEDRS
                     RDTFENAQFCAALLRPLGIKSIVLVTSANHEWRAVQEFSGAGFAVVPAPVGLYVRHKV
                     RLTSYVPNALALAASTAALYELIGDLARRAFEILHLRQHTR"
     gene            1934..2824
                     /locus_tag="VI195_01665"
     CDS             1934..2824
                     /locus_tag="VI195_01665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY6823118.1"
                     /translation="MFEDDLITVFRSAYRRECNERLLVLTAVGVDAVITAAPGEFLLQ
                     VAAADAGYAARHLLQYDAENRPAAPAPPPPPLYGHAWVGCAAYVLVLVGVAWMLSNGL
                     VRLDAFDLGELDAARVQAGEWWRAWTALTLHLDAPHLAANLGAGIWFGYLAARQMGGG
                     TAWLVTVTAAALANLLEGLLGPPEHRAVGASTAVFAALGAMSAYSWRERFALPQRWAR
                     RWGPLIAGVILLGWTGAGGEGTDVDVVAHLGGFVMGALLGATAALPSCRRLLRGVPQW
                     LLGAAALASLALAWARALAS"
     gene            complement(2937..>3840)
                     /locus_tag="VI195_01670"
     CDS             complement(2937..>3840)
                     /locus_tag="VI195_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY6823119.1"
                     /translation="SSDTPEPMKELVVEGRHEGPRMWTVRNGAHTLWILGTISPLPKK
                     MEWQPDAVEEVLKQTQEVVPAWPAYGIGANPITALRVYIAWRHLQKPPDNLPLRESLP
                     PPLYARVEALRVRYAPHDTKIEQMRPMLAARELLIKVFDAVGLAVRNEVQQEVLSLAA
                     RQGVRVHQDKLRIEDPVDVLKDVGATPLAGEVACLDAVVTLLETDVANMQARARAWAL
                     GDVDTLRQLPHADDRAACIAAVSTSERVRKLIARAQDNWLIAVTDSLARNRGTLAVQS
                     MDRLLGEHGALATLRDRGYLVEGP"
BASE COUNT          485 a         1286 c         1345 g          724 t
ORIGIN      
        1 ggacggcact gtgggcgatg gcgtgtgcgg cgccgctgtt cgtgctggcc ggggcggcgc
       61 gccatctcat tcttgccccc ttcggcttcg ataccgtggt ggagcagctg gccgcggcct
      121 tctggtttcc gcgcgtcgcg ggcgcctgca tcggagctgc ggtatgggcg atgctcgggt
      181 tcttcaacgg catcgggcgg ccgcgcacta cgctgctgat caccctggta accaccataa
      241 ccaatgcggt gctcaaccag gagttcattt tccggctcgg ctggggaata gccggctcag
      301 cgtgggcgac gaacgtcgcg cagctcgcgg gcctcgcctt cgcgctgacg ctgtttctag
      361 ccccgggcta ccgccgccgc tttggctcgc accttacctg gcgcctgcag cccgcacgtc
      421 tcatgcagca gctgcgcctc ggagtgccga tgggtctatc ccctgctgca gatctgctcg
      481 ggttcgcgat tttccagatg atgcagacgc gcctcggcac cgccggcggg gctgccacgc
      541 agatcgtcgt gatcctgaca tcgctcgcct acatgccggg tttcggtatc gcctcggcag
      601 gcaccacact cgtgggccag tcgatcggcg ccggggcgcg tgactgggcg caacgcgtcg
      661 ggcagcgagt gatcctgctc actgcctgct acatgggcgg gatcggcgtc ctgatagcac
      721 taggcggccc atgggtgttg ccgttcttca ctggcgcgca cgacgccgat gcgcgcccgg
      781 ccatcctcat cggtatgcag ctgttgtggc tcgcggccgg ctaccagttc ttcgacggcc
      841 tcaatctcgg cagcagcatg tgcctgcgtg gtgcgggtga tgtgcgcatc ccggcgctgc
      901 tggtgctcgc tctgtcgctg ctgctgttcg tgccgctggc gcatgcgctc accttcgcgc
      961 ccgggcaagg atgggtgagg ttcctgccgc agctcggctg gggcgccgtc ggcggctgga
     1021 ttgcggtact catctatatc atggtgctcg gcagcgcgct gttcctacgc tggcgctcgc
     1081 gcgcctggca gacgatccag ctttgatcgc ggaaaaagcc cgcaccatgt tcatcgcctt
     1141 gaagacactg ttgcatacgc tgctgctgcc gcccggcgga ccgctgctgc tcgccgccgc
     1201 gggtgcctgg ctcatcgccc ggggtaccgg cgggcgcctg cgccgtgccg ggtgggtgct
     1261 gctcgttgcc gcgcttgcca cgctgtggct gctggccaca ccgctcgtgg cggacgcgct
     1321 cgaggccgcc gcggagcgct gcccggcact cgatctgagc cgtccggttc cagcacaggc
     1381 cgtggtcatc ctcggcggcg ctgcagggcg gctcgacgcg cccgagtacg gcggtgagcc
     1441 ggcggcggcg tgggagctgc tcgagcggct caattacggc gcgcacatcg cccgacgcac
     1501 cggcctgccg gttgcggtgt ccggctcggg cgatgaggtg ctcgccatga acgccacgct
     1561 ggcacgtgat tttggggttg ccacgcgctg gatagaggat cggtcgcgcg acacctttga
     1621 gaatgcacag ttctgtgcag cgctgctgag gcccctcggc atcaagagca tcgtgctcgt
     1681 gaccagcgcg aatcacgagt ggcgcgcggt gcaagagttc agtggcgccg gcttcgcggt
     1741 cgtgccggca cctgttggcc tctatgtgag gcacaaggtg cgactgacga gctatgtccc
     1801 caacgccctc gcgctcgcgg cctcgacggc tgcgctctac gagctgatcg gcgacctcgc
     1861 gcgccgtgcg ttcgagatac tgcatctgcg gcagcacacg cgctgagatc acgcagctcc
     1921 gaaggcgcag cgggtgttcg aggacgatct gatcaccgtg ttccgctccg cataccggcg
     1981 cgagtgcaat gagcggctgc tcgtgctcac cgcggtcggt gtggacgccg tcatcacggc
     2041 tgcgcccggg gagtttctgc tgcaggtggc agccgctgat gcgggctacg ccgcacggca
     2101 cctgctgcaa tacgacgccg agaaccggcc cgcagcgccg gcgccaccgc caccgcccct
     2161 ttacggacac gcctgggtcg gctgtgcggc ctatgttctg gtcctcgtgg gcgttgcgtg
     2221 gatgctctcg aacgggctgg tgcggctcga tgccttcgat ctcggcgagc tcgatgcggc
     2281 gcgcgtgcag gcgggggagt ggtggcgcgc gtggaccgcg ctgacgctgc acctggatgc
     2341 gccgcatctc gcggcgaatc tcggcgctgg aatctggttc ggatacctcg ctgcccgcca
     2401 gatgggtggc ggcaccgcct ggctcgtgac ggtgacggca gcggcgctgg cgaatctgct
     2461 cgagggattg ctcgggccgc ccgagcatcg tgcggtcggc gcctcgaccg cggtgttcgc
     2521 cgcgctcgga gcgatgtcgg cttactcctg gcgggagcgg tttgctctgc cgcagcgctg
     2581 ggcgcggcgc tggggtccgc tcattgccgg ggtcattctg ctcggctgga cgggcgcggg
     2641 gggtgagggg accgacgtgg atgtcgttgc ccacctcggc ggcttcgtga tgggcgcact
     2701 gctgggtgcg accgccgctc tgccgtcctg ccgccggctg ttgcggggag tcccgcaatg
     2761 gctgttgggc gccgcggcgc tcgcctcgct cgcgctcgcc tgggcccgcg cgctcgcgag
     2821 ctgactgccg ccccggccat tccggagcag ggacgcgtct gaggttgttc tacgcaacag
     2881 ttaaccgatc ggttaaccat ttacaaagca ggcatgcact gtgcggctcc tcggccctag
     2941 ggtccttcga ccaggtagcc gcggtcgcgg agcgtggcga gcgcaccatg ctcgccgagc
     3001 agccggtcca tggactgcac cgcgagggtg ccgcgattgc gcgccaggct gtcggttacc
     3061 gcgatgagcc agttatcctg cgcgcgcgcg atgagcttgc gtacgcgctc tgaggtcgac
     3121 accgcggcaa tgcaggcggc gcggtcgtcg gcatgcggca gctggcgcag cgtatcgacg
     3181 tcgccgagcg cccaggcacg ggcgcgcgcc tgcatgttgg ccacgtcggt ctcgagcagc
     3241 gtgacgaccg catccaggca agcgacttcg ccggcgagtg gcgttgcgcc gacgtccttc
     3301 agcacatcga ccggatcttc gatgcgcagt ttgtcctgat ggacgcgcac tccctggcgc
     3361 gcggcgagac tgagcacctc ctgctgaacc tcgttgcgca ccgcgagtcc caccgcgtcg
     3421 aataccttga tgagcagctc gcgcgcggcc agcatcgggc gcatctgctc gattttcgtg
     3481 tcgtgaggcg cgtaacgcac tctcagggct tcgacgcgcg catacagcgg cggcggcagg
     3541 ctttccctca gcggcagatt gtcgggaggt ttctgcaggt gccgccaggc gatgtagacg
     3601 cgcagcgcgg tgatcgggtt ggcgccgata ccgtaggctg gccatgcggg caccacctct
     3661 tgtgtttgct tcagtacttc ctcgaccgca tccggctgcc actccatctt cttcggcagc
     3721 ggagagatcg tgccgaggat ccacagggtg tgggctccgt tgcgtaccgt ccacatgcgc
     3781 ggaccctcgt gccggccttc gaccacgagc tccttcatcg gctcgggcgt atcgctgctt
//
LOCUS       DATDMP010000098         4763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1571530, whole genome shotgun sequence.
ACCESSION   DATDMP010000098 DATDMP010000000
VERSION     DATDMP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571530"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VI195_01675"
     CDS             complement(<1..810)
                     /locus_tag="VI195_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823120.1"
                     /translation="MAAITALCSTPLSSQVPANASELMQLYQNLTPEQQDAIMKQLGG
                     GAGAGGLSSILGGLGGQGGQPGGTGLDKQGIFNRRAASSQAPVTPAEEEEPEEQLPGL
                     KGSDWVVVQADVQSPPQAPPQPAPPPVTPPPTATPSGANPAALASLLAASASAPPSAA
                     NAASAAAGSSAASAATDDQTRRLQALVDLIRAKNPYQLSPEGDLTLPGFAPIALLGLS
                     DDQATLRLSVEPGLRGLSIHLTRLSLRKTGAAGLKPFGYDLFTSGSPPSTFA"
     gene            complement(929..2056)
                     /locus_tag="VI195_01680"
     CDS             complement(929..2056)
                     /locus_tag="VI195_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6823121.1"
                     /translation="MRAAVVNAAPLVETPRRASAAPPLTPTVFFFCRLGDMVMVTRML
                     NLLHRRYGRPCQVIGTDSWTPSTYVGSPDVAGVWAFHRHLPFLLDGAWLAVRRVLRTS
                     APGPIYVCERHYRQLPRIRRMLRLSGIDPHRCVFVSDRPVRGPVQETAHLVDRLVAFG
                     ARTPANLRAEDYPAPAAEALDGPRLYVLDSERAARDARLRALGFLGSEIVLVQPGNHR
                     TMGPRRARWRRLNTDDKWWPIERWAQLLQRIHEVRPDAVLLLRGSQQEVPMLQEIAAA
                     TGLPNVATVGTTLRELYALCESASSMVSVDSGPGHAAAALSVPLVVLYGAESPLYWLP
                     RSPAGSPVVGVGGPPASRRADQVSVDAMFDAWCSIARYRCA"
     gene            complement(2057..4042)
                     /locus_tag="VI195_01685"
     CDS             complement(2057..4042)
                     /locus_tag="VI195_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEY6823122.1"
                     /translation="MKSSSRRSVFVSLCLLAASSLHARGVSPYLPLNLDPELEREVER
                     VLILGDQPVMTRPIPAARVLDALPRACAIDPLLCRRVRRALKAYMQDSALEFASVEGS
                     ATNGDSKLVIPNLHGQSEQSAWQVAGAGFLQPWDHFLVSLGGVAYDGRQKPTGSFISV
                     GFEGMQIDFGFRDHWWSPMTDSAMMISTEAPTMPSLTISNYTPLTRFGWSYEAFLARM
                     SYTDKIELTDGELTAGHPRFFGGHLGIEPAHTGWSFAANRVLVFGGGAAGGQSFKELA
                     QAFFNPTQSQTTSTGQPVLGKQEASITSRLIYPGRIPFSLYFEFAGNDTGGGNRLTFS
                     RTNLSVGLQFPRLGPFDLTYELSEWQPTWYVKPHTEVQYGYGDGITNYLLSTGHWFGD
                     QRQFGDAIGGQSNMLRLGYEPHFGGRFELTLRSLVNDSIYSAFPYEHEYMGSLMYSYP
                     LRDYIVGTQVDHGRDVFGSHYTRLSAFLRYGDALHSAAGDEEETAAVERPEGMEVHVD
                     LGAVGSDVLANITSNTPQVSSGIGFGPHLTVGARRAASAHQDVGVALEADGVHGVSLL
                     SARMIDYRYRFDNPLALNLFLGAARYAALTPAYGFYYGAGLQWRNVLPHWDVGIDLRY
                     ASKVNRIRTLPGEPQGGYRPDAYYNITLGTVYVSRKF"
     gene            complement(4123..>4763)
                     /locus_tag="VI195_01690"
     CDS             complement(4123..>4763)
                     /locus_tag="VI195_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823123.1"
                     /translation="YDDSLRRFPAYLQQLEMESNGKSVMLDGLLVESGTEAVIWGEAG
                     NNGQHSFFQLLHQGTARAALHFLLPARSSCGNQAQQNLAIASCLAQAQALTTGHSAET
                     VRTELEHQGVPAAQIDTLVPHKVDPGSRPLTLLVFRQLDPATLGRLIALYEHSVLTQS
                     VVWGINAFDQWGVELGKTLTKKIAPAVEDPSGGHSVPAAIGKLIQRIDQWRR"
BASE COUNT          836 a         1546 c         1621 g          760 t
ORIGIN      
        1 cgcaaaggtc gagggtggcg acccgctcgt gaagaggtcg tagccaaagg gcttgaggcc
       61 ggccgcgccg gtcttgcgaa gcgacagccg cgtcaagtga atgctgagcc cacgcaaccc
      121 cggctcgacc gaaaggcgca aggttgcctg gtcatcggac aggccgagca gagcgatcgg
      181 tgcgaaaccc ggcagcgtga ggtcgccctc aggcgagagc tggtatggat tcttcgcgcg
      241 gatcaggtcg acgagcgcct gcaggcgtcg ggtctgatcg tcagtcgctg ccgaggcagc
      301 cgagctgcct gccgcggcgc ttgccgcgtt agctgccgag ggtggcgcgg acgcggaggc
      361 ggcgagcagg ctcgcgagtg cagccgggtt tgcaccgctc ggcgtggcgg tcggtggtgg
      421 agtgacgggc ggcggagcgg gctgcggggg cgcctggggc ggactctgga catccgcctg
      481 aacgaccacc caatcactcc ccttgagccc cggtagctgc tcttcgggct cctcttcctc
      541 ggccggagtc accggcgcct gggaagaggc ggcccggcgg ttgaaaatcc cctgcttgtc
      601 gaggccggtg ccgcctggct ggccaccctg ccctccgagg ccgccgagaa tcgagctgag
      661 gccaccggca ccggcgccac cgcccagttg cttcatgatg gcgtcctgct gctccggtgt
      721 caggttctga tagagctgca tcagctcgct cgcgttcgcc ggcacctggg aagagagtgg
      781 ggtcgagcag agagcagtga tggcagccac gagcaaggcc gccagggaac gtcgcagctt
      841 gcacctcatc gggctatgac tgaccacgct gaccccacga accgccctcg actgcagatg
      901 gatgcgcggg attgtactgg atgcgcgctc aggcacagcg gtatcgggca atgctgcacc
      961 atgcatcgaa catggcatcg actgacacct ggtccgctcg ccgcgatgcc ggcggtccgc
     1021 ccacgccaac caccggcgag cccgcaggac tgcgtggcag ccagtagagc ggcgactcgg
     1081 caccgtagag cacgacgagc ggcacactga gtgccgcggc ggcatgcccg ggaccgctat
     1141 cgactgatac catgctgctg gcgctctcgc acagggcata cagctcgcgc agcgtcgttc
     1201 cgacggttgc cacattcggc agacccgtgg cggccgcgat ttcctgcagc atcggaactt
     1261 cctgctgcga accgcgcagc agcagcaccg cgtccgggcg cacttcatgg atccgctgca
     1321 gcaactgcgc ccagcgctcg atcggccacc atttgtcgtc ggtattcaac cttcgccagc
     1381 gcgcgcgccg cgggcccatg gtgcggtggt tgccgggctg gacgaggacg atctcactac
     1441 cgagaaagcc cagcgcgcgc aggcgcgcat cgcgcgcggc gcgctccgag tcgagcacgt
     1501 acagccgcgg gccatccaag gcttcagccg ccggcgcggg atagtcctcg gcacgcagat
     1561 tggctggcgt gcgcgctccg aacgccacca gtcggtccac caggtgcgcg gtctcctgca
     1621 ccggtccccg cacgggccgg tcgctgacaa acacgcagcg gtgcgggtcg ataccgctca
     1681 agcgcagcat gcggcggatg cggggcagct gccggtagtg acgctcgcac acgtagatcg
     1741 gacccggtgc actggtgcgc agcacccgcc gcactgcaag ccatgcgcca tccagcagga
     1801 aaggcaggtg acgatggaac gcccagacac cggcgacatc gggactaccg acataggtgc
     1861 tcggcgtcca ggagtccgta ccgatgacct gacatggccg cccgtagcgc cgatgcagca
     1921 ggttcagcat ccgcgtaacc atcaccatat cgcccagacg gcagaagaaa aacacggtcg
     1981 gggtgagcgg cggcgcagcc gaggctcgtc tgggcgtttc cacgagcggc gcggcattca
     2041 cgacggcagc gcgcactcaa aacttgcgcg acacgtagac ggtgccgagc gtgatgttgt
     2101 aataggcatc tggccggtaa ccgccctgcg gctcacccgg cagagtccgt atgcggttca
     2161 ccttggacgc gtagcggagg tcgatgccga cgtcccagtg cggcagcacg ttgcgccatt
     2221 gcagacccgc tccgtagtag aacccgtatg ccggagtgag cgccgcatag cgtgcagcac
     2281 cgaggaacag gttgagcgcc agcggattgt cgaagcggta acgatagtcg atcatgcgcg
     2341 cactgagcag cgatacgccg tgaacgccat ccgcttcgag cgcaacgccg acatcctggt
     2401 gcgcgctcgc ggcgcggcgc gcgccgacag tcaggtgcgg cccgaaaccg atacccgagc
     2461 tcacctgcgg agtgttggac gtgatgttgg cgagcacgtc gctgccgacc gcgccgaggt
     2521 caacatgtac ctccatgccc tccgggcgct caaccgctgc ggtctcctct tcgtcgcctg
     2581 cagcggagtg caacgcatcg ccatagcgca ggaaagcgct gaggcgtgtg tagtgggagc
     2641 cgaacacgtc ccgcccgtga tccacctgcg tcccgacaat gtaatcgcgc agcgggtagg
     2701 agtacatcaa cgaccccatg tactcgtgct cgtacggaaa ggctgaatat attgaatcgt
     2761 tgaccagcga ccgcagtgtc agctcgaatc gcccgccgaa gtgaggctcg tagccgagcc
     2821 gcagcatatt gctctgacca ccgatggcat cgccgaactg gcgctggtcg ccgaaccagt
     2881 ggccggtgct cagcaggtaa ttggtgatgc cgtcgccgta gccgtactgc acttccgtgt
     2941 ggggtttgac ataccaggtc ggttgccatt cggagagctc gtatgtcagg tcgaatggcc
     3001 cgaggcgcgg gaactgcagg cccactgaga gattggtcct ggagaaggtc agacgattgc
     3061 cgccaccggt atcgttaccg gcgaattcga agtacagcga gaacggaata cggccggggt
     3121 agatgaggcg gctggtgatc gaagcctcct gcttgccgag aaccggctgg ccggtgctgg
     3181 tcgtctggga ttgcgtgggg ttgaagaacg cctgcgccag ctccttgaac gattgaccgc
     3241 cggcagcgcc gccgccgaaa accaggacgc ggttggcggc gaacgaccag ccggtatgcg
     3301 ccggctcgat gcccaggtgt ccaccgaaga atctgggatg gcccgccgtc agctcgccgt
     3361 cggtgagctc gatcttgtcg gtgtaggaca tgcgcgcgag gaacgcttcg tacgaccagc
     3421 cgaaccgcgt gaggggggtg tagttcgaga tcgtgagcga gggcatcgtc ggcgcctcgg
     3481 tactgatcat catggcgctg tcggtcatgg gcgaccacca gtgatcgcga aagccgaaat
     3541 cgatctgcat cccctcgaag ccaacgctga tgaaggagcc ggtcggcttc tgccggccgt
     3601 cataggcgac accgccgagg ctcacgagga agtggtccca gggctggaga aaaccggcgc
     3661 cggcgacctg ccacgcactc tgctcgctct gcccgtgcag attcggaatc accagcttcg
     3721 agtcgccatt cgtcgccgag ccttcgacgc tcgcgaactc gagcgccgaa tcctgcatgt
     3781 acgccttcag cgcccggcgc acgcggcggc agagcagcgg gtcgatggcg cacgcccgcg
     3841 gcagtgcatc gagcaccctc gccgcgggaa tgggtcgtgt catgaccggc tgatccccga
     3901 gaatcagtac gcgctccact tcgcgttcca gctcggggtc gagattgagc ggcaggtaag
     3961 ggctcacgcc ccgcgcgtgc agggagctgg cggcaagcag gcatagcgac acgaatacgc
     4021 ttcgacgcga gctactcttc atcgatccgc ttcaccgggg cgcctctgcg gccgcgcgct
     4081 gctgcaaaag cgcgcactat actgtgggcg cacccgcggc gctcagcgcc gccactgatc
     4141 gatgcgctgg atgagtttgc cgatggcggc cggcactgag tgcccgccgc tcgggtcctc
     4201 gaccgccggg gcgatcttct tggtgagcgt cttgccgagt tccacccccc actggtcgaa
     4261 ggcattgatg ccccacacga cgctctgcgt gagcacgctg tgctcgtaga gcgcgatgag
     4321 gcggccaagg gttgccgggt cgagctgccg gaacaccaag agggtgagcg ggcggcttcc
     4381 ggggtcgacc ttgtggggaa cgagggtgtc gatctgcgcc gccggcactc cctggtgctc
     4441 gagctcggtg cgcacggtct ccgcggagtg gccggtggtg agcgcttgcg cctgggccag
     4501 gcagctcgcg atcgcgagat tctgctgcgc ctggttgccg caggaagagc gtgccggcaa
     4561 caggaagtgc agcgcagcgc gcgccgttcc ctggtgcagc agctggaaga acgagtgctg
     4621 gccgttgttg ccggcctccc cccagatgac cgcttcggtg ccggattcca ccaacagccc
     4681 gtcgagcatg accgacttgc cattgctctc catctccagc tgctgcaggt aggcggggaa
     4741 gcgccgcagt gaatcatcgt acg
//
LOCUS       DATDMP010000099         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1127124, whole genome shotgun sequence.
ACCESSION   DATDMP010000099 DATDMP010000000
VERSION     DATDMP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127124"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VI195_01695"
     CDS             complement(<1..310)
                     /locus_tag="VI195_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEY6823124.1"
                     /translation="MAEARVPAIRTVSIVGARPQFVKLSPVSRAMRQARRAISDEIVH
                     TGQHYDAAMSSVFFDELDIPAPAVNLGIGSASHGRQTARMLEAIEAYLQQQRPDVVLV
                     Y"
     gene            complement(311..892)
                     /locus_tag="VI195_01700"
     CDS             complement(311..892)
                     /locus_tag="VI195_01700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY6823125.1"
                     /translation="MVTIHPTAIIDAGATIGDGTRIWHWVHVCAGARIGTGCSLGQNV
                     FVGNRVVIGSNVKIQNNVSVYDNVTLEDDVFCGPSMVFTNVYNPRAAVSRKDAYRDTL
                     VRRGATLGANCTIVCGTTIGEYAFIGAGAVINREVAPFALMAGVPARQLGWMSRFGER
                     LGLPLRGDGQARCAATGDVYLLRDGVCVLAGEA"
     gene            complement(895..1956)
                     /locus_tag="VI195_01705"
     CDS             complement(895..1956)
                     /locus_tag="VI195_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6823126.1"
                     /translation="MNHAYDPIVGRKIRVALTGCGRIAAKHFEALQAHRERTELVAVC
                     DTDRAALAAAVAATGARGFPSLNALLEGCDADVVVLCTPSGEHPAQSIQIAAAGRHVI
                     TEKPMATRWADGKRMVEACDAANVRLFVVKQNRRNATLQLLKRAVEQKRFGRIYMVAL
                     NVFWSRPQSYYDSARWRGTWEFDGGALMNQASHYVDLLDWLIGPVESVQAYTATLARD
                     IQVEDTAVVGIRWRSGALGSMSVTMLTYPRNYEGSITVLGETGTVLVGGVAVNDIQHW
                     EFAEPQDYDAQVRDASYAATSVYGFGHALYYDNVIRTLRGEAEPETDGREGLRSLEVL
                     IASYLSARDGRRVALPLEY"
     gene            complement(1953..2300)
                     /locus_tag="VI195_01710"
     CDS             complement(1953..2300)
                     /locus_tag="VI195_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HEY6823127.1"
                     /translation="MLSDEMRLKLMRLLEANPKASQREVAREFGISVGKVNYCLQALV
                     QRGWVKANRFRNSRNKSAYRYLLTPRGIEAKAALTLRFLRIKVREYEALRTEIEQLRR
                     ETAVRQAGGPEQP"
     gene            complement(2424..>2755)
                     /locus_tag="VI195_01715"
     CDS             complement(2424..>2755)
                     /locus_tag="VI195_01715"
                     /inference="COORDINATES: protein motif:HMM:NF018010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HEY6823128.1"
                     /translation="QVTVIGEVQNVTSHLYNPKLGREDYIALSGGVTRRADRSKIYIV
                     RASGSVIAAEGSRWFERGGNIAIKPGDTIVVPLDTEHIPALPLWTQVTQILYNIAIAV
                     LAVHSIA"
BASE COUNT          460 a          937 c          877 g          481 t
ORIGIN      
        1 catagaccag cacgacatcc gggcgctgct gctgcagata ggcctcgatg gcctccagca
       61 tgcgcgccgt ctgccgtccg tgcgaggcgg agccgatgcc cagattcacc gccggcgctg
      121 ggatatccag ctcatcgaaa aagacgctcg acatggcagc gtcgtagtgt tgtccggtgt
      181 gcacgatctc atcgctgatc gcgcggcgcg cctggcgcat ggcacgggac accggcgaga
      241 gcttcacgaa ctgcgggcgg gcaccgacga tgctgacggt ccggatcgcg ggcacacgcg
      301 cctcggccac tcaggcttct cccgctagca cacagactcc atcacgcagc aggtaaacat
      361 cgccggtagc cgcgcagcgc gcctggccat cgccgcgcag cggcagcccg agacgctcgc
      421 cgaagcggct catccagccg agctgccgtg ccgggacgcc cgccattagc gcaaacggcg
      481 cgacctcgcg gttgatcacc gcaccggcgc cgatgaaggc gtattcaccg atggtcgtgc
      541 cgcagacgat ggtgcaattc gcgccgagcg tggcgccgcg gcgcaccagc gtgtcgcggt
      601 acgcgtcttt gcggcttacc gctgcacgtg gattgtagac gttggtaaac accatgctcg
      661 gtccgcagaa cacgtcgtcc tccagggtga cgttgtcgta caccgagacg ttgttctgga
      721 tcttgacgtt gctgccgatc accacgcggt tgccgacgaa cacgttctga ccgagcgagc
      781 agcccgtgcc gatgcgcgca cccgcgcaca cgtgtaccca gtgccagatg cgcgtgccat
      841 cgccgatggt cgcgcccgcg tcgatgatgg cggttggatg gatggtgacc aacctcaata
      901 ctccagcggc agcgccacgc gtcgtccatc gcgcgccgag aggtagctgg cgatcagcac
      961 ctcgagcgag cgcaggccct cccgcccgtc ggtctcgggc tcagcctcac cgcgcagggt
     1021 gcggatgacg ttgtcgtagt agagcgcatg cccgaagccg taaaccgagg tggcggcgta
     1081 actcgcgtcg cgcacctgtg cgtcatagtc ctgcggctcg gcgaattccc agtgctggat
     1141 atcgttgacc gcgacaccgc cgacgagcac ggtacccgtc tcgccgagca cggtgatcga
     1201 gccctcgtag ttacgtgggt aggtgagcat ggtgacgctc atcgagccga gcgcgccgga
     1261 gcgccagcga atgcccacca ccgccgtatc ctccacctgg atatcccgcg ccagcgtcgc
     1321 cgtgtaggcc tgcacgctct cgaccggacc gatcagccag tcgagcagat cgacgtagtg
     1381 actcgcctgg ttcatgagcg caccgccatc gaattcccag gtgccgcgcc agcgcgcgct
     1441 gtcgtaatag ctctgcggcc ggctccagaa cacattcagc gccaccatat agatgcgccc
     1501 gaagcgcttc tgctcgaccg cacgtttcag cagctgcagc gtggcgttgc ggcggttctg
     1561 cttgaccacg aacaggcgca cgttggccgc atcgcacgcc tccaccatgc gtttgccgtc
     1621 cgcccagcgc gtcgccatgg gtttctcggt aatgacgtgg cggccggcgg cggcgatctg
     1681 aatgctctgt gcaggatgct cgccgctcgg ggtacacagc accacgacgt cggcatcgca
     1741 accctcgagc aacgcattca gcgagggaaa gccgcgcgcg ccggtggcgg ccacagcggc
     1801 cgcgagcgcc gcacggtcgg tgtcgcacac cgccaccagc tcggtgcgct cgcgatgcgc
     1861 ctgcagcgcc tcgaagtgct tcgcggcaat gcggccgcag ccggtgagtg cgacgcgaat
     1921 cttgcgcccg acgatcgggt cgtaggcatg gttcatggct gctccggtcc tcccgcctgg
     1981 cgcacggcgg tttcgcggcg caactgctcg atctcggtgc gcaacgcctc gtactcgcgc
     2041 acttttatgc gcaggaagcg cagcgtcagt gccgctttgg cttcgatgcc gcgcggagtc
     2101 agtagataac ggtacgcgct cttgttgcga ctgttcctga agcgattcgc cttgacccag
     2161 ccgcgctgca ccagcgcctg caggcagtag ttgaccttgc ccacgctgat gccgaattca
     2221 cgcgccacct cgcgttggct cgccttgggg ttggcctcga gcagcctcat cagcttcagg
     2281 cgcatttcat cgctcagcat gggctcctgt catagcgtgc cgcgccgccg cagccgccga
     2341 tgtgttcaac agttgaacgc aaggctaaga gcgttggcgc gcggagtcaa ccaacggggg
     2401 cgcgcgcgcc tgtgccgggc gagtcaggca atggaatgga ctgccagtac cgcgatcgcg
     2461 atgttgtaga gaatctgcgt aacctgcgtc cacaacggca gcgccggaat gtgctcggtg
     2521 tcgagcggca cgacgatggt gtcacccggc ttgatggcga tgttcccacc gcgctcgaac
     2581 cagcggcttc cctcagccgc gatcacgctg ccactcgcgc gcacgatgta gatcttgctg
     2641 cggtcggctc ttcgcgtcac accaccgctg agggcgatat agtcttcgcg tccgagcttc
     2701 ggattgtaga ggtgcgaagt cacattctgc acctcgccga tgacggtcac ctgct
//
LOCUS       DATDMP010000100         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1016197, whole genome shotgun sequence.
ACCESSION   DATDMP010000100 DATDMP010000000
VERSION     DATDMP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016197"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VI195_01720"
     CDS             <1..186
                     /locus_tag="VI195_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823129.1"
                     /translation="LRNYVVARINPVRFHKAKKGDTAPPMPLAQALTRMAAAARDFNV
                     ASVSNSDLAWVAVGAGE"
     gene            complement(213..>2177)
                     /locus_tag="VI195_01725"
     CDS             complement(213..>2177)
                     /locus_tag="VI195_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEY6823130.1"
                     /translation="AACVLAVAVAAVPAARAGLNGAAVVHPTRWSKLHSPLPRDPLLD
                     ARIEQLLADMTPAQKVGQLIQADIGSITPDDLRHYPLGSILNGGNSSPRDDKLAAPSE
                     WLSLADGFYEASIADSPEGLPILWGTDAVHGHNNIPGATLFPHNIGLGAARDPQLIRR
                     IGEITALEVRVTGLDWAFSPTVAVARDARWGRTYESYAENPGLVREYAAAMVEGLQGA
                     PGTPQFLDAAHVLATAKHFLGDGGTVGGHDEGDNTAGESELRDIDAAGYEAAVVAGVQ
                     TIMVSYSSWQGMKMHGNHALLTEVLKRRMGFDGLLIGDWNGHAQVPGCTAVRCAPAVD
                     AGVDVLMAPDGWRELHGNLLREVRAGQIPAQRLDDAVRRVLRVKLRAHLDSEGRPSAR
                     PFAGHYELLGAPAHRAVARQAVRESLVLLKNRHHLLPLSPHAHVLVAGDAADSIARQS
                     GGWTIDWQGTEPNQDFPHAETIYAGIAQCVQAAGGSATLSPEGTFTERPEVAIVVFGE
                     HPYAESAGDVTTLEYSPADKHDLALLRRLRDQNIPVVAVFLSGRPLWINAELNAADSL
                     IAAWLPGLEGGGVADVLFRAADGAIRYDFRGRLSFSWPNSPRPTAIGQRRGEPPLFPY
                     GYGLSYRDDGELPQLPESEAAQALPRHARR"
BASE COUNT          348 a          768 c          728 g          333 t
ORIGIN      
        1 ctgcgcaact acgtcgtggc gcgcatcaac ccggtgcgct tccacaaggc gaagaaaggt
       61 gacactgccc cgccgatgcc actggcgcag gccctcacgc gaatggcagc cgcggcgcgc
      121 gacttcaacg tcgcctcggt atccaactct gacctcgcgt gggtggcagt cggcgcgggc
      181 gagtagcacc gccatccgcc gggtgccggc gcttaccgcc tagcgtgccg cggcagcgcc
      241 tgcgctgcct cgctctcggg caactggggc agctcgccgt cgtcacggta gctgaggccg
      301 taaccgtagg gaaaaagcgg cggctcgccg cgacgctgcc cgatcgcggt gggacgcggt
      361 gaattcggcc aggaaaacga caggcggccg cggaagtcat agcgtatggc tccatcggcc
      421 gcgcggaaca gcacatcggc aacaccgccg ccctccagcc ccggcagcca ggcggcgatg
      481 aggctatcgg cggcgttgag ctcggcattg atccacagcg gccgtcccga cagaaatact
      541 gcgaccaccg ggatgttctg gtcccgcaac cggcgcagca gtgcgaggtc gtgcttgtcc
      601 gcggggctgt attcgagcgt cgtcacatcg cccgcggact ccgcatacgg atgctcgcca
      661 aacacgacaa tggcaacctc gggccgttcg gtgaaggtac cttcgggact cagcgttgcg
      721 ctgccaccgg ccgcctgcac gcattgtgca atgcccgcgt agatcgtctc ggcgtgcgga
      781 aagtcctgat tcggctcggt cccctgccag tcgatggtcc agccgccact ctggcgcgcg
      841 atgctgtcgg cggcatcgcc ggcgaccagc acgtgcgcgt gcggcgacag cggcagcagg
      901 tgatggcggt tcttcagcag caccagcgac tcgcgcacgg cctgacgcgc gactgcacgg
      961 tgtgcgggcg ccccgagcag ctcgtaatgc ccggcgaagg gccgcgccga ggggcgtcct
     1021 tcgctatcga ggtgcgcacg cagcttgacc cgcagcacgc gccgcacggc atcatcgagg
     1081 cgctgcgccg gtatttgccc agcgcgcacc tcccgcagca ggttgccgtg cagctcacgc
     1141 cagccatcgg gcgccatgag cacatcgacc ccggcatcca cggccggcgc gcagcggacc
     1201 gccgtgcatc ccggcacctg tgcgtggccg ttccagtcgc cgatcagcaa gccatcgaaa
     1261 cccatgcgcc tcttgagcac ctcggtgagc agcgcgtggt taccgtgcat cttcattccc
     1321 tgccagctgg agtaggacac catgatggtc tggaccccgg cgacgaccgc ggcttcgtag
     1381 ccggcggcgt cgatgtcgcg cagctcactc tcgcccgcgg tgttatcgcc ctcatcgtgg
     1441 ccacccacgg tgcccccgtc gccgaggaaa tgcttggcag ttgccagtac gtgtgcggca
     1501 tccaggaact gcggcgtccc gggcgcgcct tgcaacccct ccaccatcgc ggcggcgtac
     1561 tcgcggacca ggcccgggtt ctcggcatag ctctcgtagg tgcgccccca gcgcgcatcg
     1621 cgcgccacgg ccaccgtcgg ggagaaggcc cagtcgagcc ccgtgacccg gacctcgagc
     1681 gccgtgatct cgccaatgcg gcggatgagc tgtggatcgc gtgccgcccc cagcccaatg
     1741 ttgtgcggaa acagcgtggc gccggggatg ttgttgtggc catgcacggc atcggtgccc
     1801 cacagtattg gcagcccctc gggggaatcg gcaatcgaag cctcatagaa cccgtctgcg
     1861 aggctcagcc actcactcgg tgccgcgagc ttgtcgtcgc ggggcgagga gttgccgcca
     1921 ttcaggatgg aaccgagggg atagtgccgc agatcatccg gagtgatgct gccgatatcg
     1981 gcctggatca gctgccccac cttctgtgcg ggggtcatgt ccgcgagcag ctgctcgatg
     2041 cgggcatcga gcagcggatc acgcggcagc ggggagtgca gcttcgacca gcgcgtcgga
     2101 tgtacgaccg ccgctccatt gaggcccgcg cgcgccgccg ggacagcggc cacagctacc
     2161 gccagcacgc aggccgc
//
LOCUS       DATDMP010000101         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2127735, whole genome shotgun sequence.
ACCESSION   DATDMP010000101 DATDMP010000000
VERSION     DATDMP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127735"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VI195_01730"
     CDS             <1..1492
                     /locus_tag="VI195_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6823131.1"
                     /translation="VLRQARLNRGYSTVDAEAGKPLALRVIVPVGSSALADEARFLQL
                     RQTVPPAFARSAEAVETAYRDYRQLAISREGLKRIYIVTLSFALLMALFVAVAVAATQ
                     SNLLAEPLANLAQATQAVARGDFSRTTPVTSRDELGVLTESFNSMTRQLGEARRGVEA
                     NRVALEAAKADLENILANLSTGVLVFDRGLQLTISNHGAAAILGADAESFANEMRAQF
                     VAHGQKDWQLELSLKGTGKTLHARGAALPSTTGAGYVLVFDDITQLIQAQRATAWAEV
                     ARRLAHEIKNPLTPIQLSAERLEMKLGERLPREDAETLRRATGTIVSQVAALKAMVDA
                     FRDYARLPAPVPATLDLNALVAEVLALYENSSVPITKRLAERLPPVWADGAQIRQVIH
                     NLVQNAQDALENAPRAAVRPSIEVSTELAGPQARLAVSDNGAGFPEELMARIFEPYVT
                     TKARGTGLGLAIVKKIVDEHQGSITVENRPNSGASVSVLLPLSTVA"
     gene            1495..2742
                     /locus_tag="VI195_01735"
     CDS             1495..2742
                     /locus_tag="VI195_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6823132.1"
                     /translation="MASILVVDDEVGIRELLSEILADEGHQVTLAESAGDARRLRERA
                     RPDLVLLDIWMPDTDGITLLKEWSASGQLTMPVVMMSGHGTIETAVEATRIGALDFLE
                     KPIALQRLLATVKRALRNTEAAATPPLGLAALGRSAALAEAKKRLAQLALSAAPLLMR
                     GERGMQPELFARLLLAPGAPFQSGHEALSEPPSELLARTAGGIVFLPDLTKLGRNEQR
                     QLEFLLPRAERHKVRIVGFSPLDMRALAEKHDFDPELAARLAQLTFKLPALREFSEDV
                     PDLASLMLAQLVEARACPPKRLSIAALNALRHHSWPGNLLELESVVKDLALTSLEDEV
                     RLEDVERVFAARASPGALPEVAFDRPYREAREAFERLYFEYLLSRENGSMSRVAERSG
                     LERTHLYRKLKALGLPVGRREES"
     gene            complement(2739..2867)
                     /locus_tag="VI195_01740"
     CDS             complement(2739..2867)
                     /locus_tag="VI195_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY6823133.1"
                     /translation="MLFSGSLYALALGAPRGLGMMTPFGGLALIAAWLCLGLWALR"
BASE COUNT          469 a          968 c          976 g          463 t
ORIGIN      
        1 tgtgctgcgg caggcgcggc tgaaccgcgg ctattccacg gtggatgcgg aggccggcaa
       61 gcccctggca ctgcgcgtga tcgtgccggt gggcagcagc gcgcttgccg atgaggcgcg
      121 tttcctgcag ctgcggcaga cggtgccacc cgcattcgcg cgcagcgcgg aggcggtgga
      181 gacggcttat cgggactacc ggcagctcgc catttcccgc gaagggttga agcgcatcta
      241 tatcgtgacg ctgtccttcg ctctgctcat ggctttgttc gtcgccgttg cggtggcggc
      301 tacgcaatcc aacttgctgg ccgagccgct cgccaacctt gcgcaagcga cgcaagcggt
      361 tgcgcgcggc gatttttcgc gtacgacgcc cgtcacgagc agggacgagc tcggcgtgct
      421 gaccgaatcc ttcaactcca tgacgcgcca gctcggcgag gcgcgccgcg gcgtggaggc
      481 caatcgtgtc gcgcttgaag ccgcgaaggc cgacctcgag aatatcctcg cgaacctgtc
      541 gaccggcgtg ctcgtgttcg atcgagggct gcagcttacg atttcgaacc acggcgccgc
      601 ggccattttg ggcgcggacg ccgaatcctt cgcgaacgag atgcgcgcgc agttcgtcgc
      661 ccacgggcaa aaggactggc agctcgagct ctccctgaag ggcaccggca agacgctgca
      721 cgcccgcggg gcggcgctgc ccagcacgac cggcgcaggc tatgtgctgg tatttgacga
      781 catcacgcag ctcatccagg cgcagcgcgc caccgcctgg gcggaggtcg cgcggcggct
      841 cgcgcacgag attaagaacc cgctcacccc gattcagctc tctgcggagc gtttggagat
      901 gaagcttggc gagcgcctgc cccgggagga cgcggagacg ctcaggcggg ctaccggtac
      961 gatcgtcagc caggtcgcag cgctcaaggc gatggtggat gcctttcgcg attacgcgag
     1021 gctgcccgcg ccggtccccg cgacgctcga cctgaatgct ctcgtggcgg aagttctcgc
     1081 cctgtacgag aattcaagcg tgccgatcac caagcgcctg gccgagcgtc tgccgccggt
     1141 ctgggcggat ggcgcgcaaa tccgtcaagt gatccataac ctggtacaaa atgcgcagga
     1201 tgccctggag aatgcgccgc gcgcggccgt ccggccttcc atcgaagtga gtaccgagct
     1261 cgccggcccg caagcgcgcc tcgcggtatc cgacaatggc gcgggttttc cggaggagct
     1321 gatggcgcgc atcttcgagc cttacgttac gacgaaggcg cgcggcacgg gcctcggcct
     1381 cgccatcgtg aagaaaatcg tggatgagca tcaaggcagc atcaccgtgg agaatcggcc
     1441 gaacagcggt gcctcggtga gcgtgcttct gccactgtcg acggtggcct gaccatggct
     1501 tcgattctcg tggtcgacga cgaagtaggc atccgcgagc tcctgtccga gatccttgcg
     1561 gacgagggcc accaggtcac tttggccgag agtgcaggcg acgcgcgtcg gttgcgcgag
     1621 cgcgcccggc cggatctcgt gctgctcgat atctggatgc cggatacgga cggcattacc
     1681 ctgctcaagg aatggtccgc aagcgggcag ctcacaatgc cggtcgtcat gatgtcaggc
     1741 cacggcacga tcgagacggc ggtggaagcg acgcggatcg gagccctgga tttcctggag
     1801 aaaccgatcg cgttgcagcg gctccttgcg acggtaaagc gcgcgctgcg caatacggag
     1861 gcggccgcca ccccgccgct cgggctcgcg gccctcgggc gctcggcggc tctcgccgag
     1921 gcgaagaagc gcctcgcgca acttgcgctt tccgcggctc cgctcctgat gcgtggggag
     1981 cgtggcatgc agcccgagct tttcgcgcgg ctgcttctcg cacccggcgc gccgtttcag
     2041 tcgggtcacg aggcgctgtc cgagccgccc tccgagctcc tggcccgcac cgcaggcggg
     2101 atcgtcttcc tgccggacct gacgaagctc ggccgcaacg agcagcgcca gctcgagttc
     2161 ctcctgccgc gtgccgagcg ccataaggtc cgcatcgtgg ggttctcgcc cctagatatg
     2221 cgcgcgctcg cggaaaaaca cgacttcgat ccagagctcg ccgcgcgcct ggcgcagctc
     2281 accttcaagc tcccggcgct gcgcgaattc tccgaggacg ttcccgacct ggcgtccctc
     2341 atgctggcgc agctcgtgga ggcgcgcgcg tgcccaccca agcgtctctc catcgccgcc
     2401 ctgaacgcct tgcggcacca ctcctggccg ggcaatctcc tcgagctgga gagcgtggtg
     2461 aaggacctcg cgctcacctc gctggaggac gaggtccggc tggaagacgt cgagcgcgtg
     2521 ttcgcagcca gggcctcccc cggcgcgctg cccgaggtcg cgttcgatcg cccctaccgg
     2581 gaggcgcgcg aggctttcga gcggctgtac ttcgaatacc tgctctcgcg cgagaacggc
     2641 agcatgtcgc gcgtggcgga gcgctcgggc ctcgagcgca cacacctgta ccgcaagctg
     2701 aaggcgctag ggctcccggt gggccggcgc gaggaaagct agcgcaatgc ccacaggccg
     2761 aggcacagcc aggcggcgat aagcgccagc ccgccgaagg gcgtcatcat gccgaggcca
     2821 cgcggcgcgc cgagggcgag tgcgtaaagg ctgccggaaa aaagcaagat cccgcc
//
LOCUS       DATDMP010000102         3285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_794780, whole genome shotgun sequence.
ACCESSION   DATDMP010000102 DATDMP010000000
VERSION     DATDMP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794780"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VI195_01745"
     CDS             <1..1299
                     /locus_tag="VI195_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6823134.1"
                     /translation="ATVPSTERVADMISMAAREAFNGAPGPVYLEIPRDVLDREVERA
                     KAIVPKPGHYRASTRSSGDPRDIEKLADLLVSSERPAILYGQQVWTARGQEEAVALLR
                     GLDIPGYFNGGSRGLLAPGDPHHFDRTRGNAFAEADVIVIVGTPFDFRMGYGRRISNK
                     AQLVQIDLDYRTVGKNRDIDLGLVGHPGAILGAVLQAASGRLKQDKREKRREWMKRLG
                     DAEAAATEKLMPLFKSNNQPIHPYRVAYELNEFLADNTIYIGDGGDVVTISAQAVRPR
                     RPGQWMDPGALGSLGVGTGFAIAAGLAHSDKEVLCYYGDGSFGMTAFDMETANRFGVP
                     YIAVIGNNSAMNQIRYGQLAKYGEERGNVGNLLGDVPFGKFAEMLGGYGEEVRDPAQI
                     APALRRAREAVQRTRRSAVINIWVDPSEYAPGTKNQTMYK"
     gene            1313..2560
                     /gene="frc"
                     /locus_tag="VI195_01750"
     CDS             1313..2560
                     /gene="frc"
                     /locus_tag="VI195_01750"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEY6823135.1"
                     /translation="MAKALDGVRILDFTHVQSGPTCTQLLAWLGADVIKVERAGAGDI
                     TREQLRDIEGVDSLYFTMLNHNKRSITLDTKKPQGKAVLEKLVRYCDVLVENFAPGAL
                     DRMGFTWERIQQLNPKMIVASVKGFGPGPYEECKVYENVAQCAGGSASTTGFDDGPPL
                     VTGAQIGDSGTGLHLALGIVAALYQRQSSGRGQRVLCAMQDGVLNLCRVKLRDQQRLA
                     RAGVMKEYPQYPDGKFGSAVPRAGNASGGGQPGWIIRCKGWESDPNAYLYFITQAPVW
                     KEICEVIGKEEWITDPDYATPEARLPRLKQVFDTIEKWTMTKTKFEAMEELNQHDIPC
                     GPILSMEELAAEPSLRATGTVVEVDHPKRGKYLSVGNPIKMSDSPTEVRRSPLLGEHT
                     NEVLKELGLSAPDIDALRTAKVI"
     gene            2557..>3285
                     /locus_tag="VI195_01755"
     CDS             2557..>3285
                     /locus_tag="VI195_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY6823136.1"
                     /translation="MSGAHLKDIDAANDPGEAMAYDKTTVRRVLDQAKSAGRSSLTAP
                     EAKEICEAYGIAIPREAVATTATEAARLASSIGFPVVMKIVSPQILHKTEAGGVVVGV
                     KSAEAAQEAFNTIVANARRYKSDAAIDGVQVQQMLAGGQEVIIGAVTDPAFGKLIAFG
                     LGGVLVEVLKDITFRLAPAMRADALSMLDSIAAAEILRGVRGSEAVNREALATLIVNV
                     SQLVTDFPEISELDLNPVFATAKGA"
BASE COUNT          620 a         1044 c         1111 g          510 t
ORIGIN      
        1 gccaccgtgc cctccaccga gcgcgtggcg gacatgatct cgatggcggc gcgcgaagcg
       61 ttcaacggcg caccggggcc ggtctacctc gagatcccgc gcgatgtgct cgaccgcgag
      121 gtggagcggg ccaaggctat cgtgccgaag cccggccact accgtgcctc gacccgctcg
      181 tccggggatc cgcgcgacat cgagaagctc gccgacctgc tggtgagctc ggagcgcccg
      241 gcgatcctct acgggcagca agtgtggacg gcgcgcggcc aggaggaggc ggtagcgctg
      301 ctgcgcggcc tcgacatccc cggttacttc aacggcggca gccgcggcct gctggcgccg
      361 ggcgatccgc atcacttcga ccgtacacgc ggcaatgcct tcgcggaagc ggatgtcatc
      421 gtgatcgtcg gcaccccgtt cgacttccgc atgggctacg gccggcgcat cagcaacaaa
      481 gcgcagctgg tgcagatcga cttggactac cgcacggttg gcaaaaaccg cgatatcgac
      541 ttggggctcg tcggccatcc gggcgcgatc ctcggcgcgg tgctgcaggc ggcatcgggc
      601 aggctcaagc aggacaagcg cgagaagcgg cgcgagtgga tgaagcggct cggtgatgcc
      661 gaagctgcgg ccaccgagaa gctgatgccg ctcttcaagt ccaacaatca gccgatccat
      721 ccgtaccgag tggcgtacga gctcaacgag tttctcgccg acaacaccat ctatattggc
      781 gatggcggcg atgtggtgac gatctccgcg caggcggtgc gcccgcgccg accggggcag
      841 tggatggacc ccggcgcgct cggctcgctc ggggtcggca ccggtttcgc catcgcggcg
      901 ggcctcgcgc attccgacaa ggaggtgctc tgctactacg gcgacggctc cttcggcatg
      961 accgccttcg acatggagac cgcgaaccgc tttggcgtgc cgtacatcgc ggtgatcggc
     1021 aacaactcgg cgatgaacca gatccgctac ggccagctgg ccaaatacgg cgaggagcgc
     1081 ggcaacgtcg gcaatctcct gggtgatgtg ccctttggca agtttgcgga gatgctcggc
     1141 ggctacggcg aggaggtacg cgatccggcg cagatcgcgc cggcgctgcg ccgcgcacgc
     1201 gaggcggtcc agcgcacgcg acgctcggcg gtcatcaaca tctgggtcga cccgagcgag
     1261 tacgcacccg gtaccaagaa ccagacgatg tacaagtgaa gcggagaccc ccgtggccaa
     1321 ggcactagat ggcgtccgca ttctcgactt cacgcacgtg cagtcgggac ccacttgcac
     1381 gcagttgctg gcgtggctcg gcgcggatgt gatcaaggtg gagcgagccg gcgccggcga
     1441 tatcacgcgc gagcagctgc gagacatcga gggcgtcgac agcctgtatt tcacgatgct
     1501 gaaccacaac aagcgctcga tcacgctcga caccaagaag ccgcagggca aggcggtgct
     1561 ggaaaagctc gtcaggtact gcgacgtgtt ggtcgaaaac ttcgccccgg gcgcgctcga
     1621 tcgcatgggc ttcacctggg agcgcatcca gcagctgaac ccgaagatga ttgtcgcttc
     1681 ggtgaagggc ttcggacccg gtccgtacga agagtgcaag gtctacgaga acgtcgcgca
     1741 gtgtgccggc ggctcagcct ccaccaccgg cttcgatgac ggcccaccgc tcgtcaccgg
     1801 cgcgcagatc ggcgacagcg gcaccgggct ccatctcgcg ctcggcatcg tggcggcgct
     1861 ctatcagcgc cagagcagtg ggcgcggtca gcgcgtgctg tgcgccatgc aggatggggt
     1921 gctgaacctt tgccgcgtca agctgcgcga tcagcagcgc ctcgcgcgcg ccggcgtcat
     1981 gaaggagtac ccgcagtatc cggatggcaa gttcggcagc gcggtgccgc gcgccggcaa
     2041 cgcctcgggc ggggggcagc ccggctggat catccgctgc aagggctggg agagcgatcc
     2101 caacgcctac ctctacttca taactcaggc gccggtgtgg aaggagatct gcgaggtgat
     2161 cggcaaggaa gagtggatca ccgatcccga ctacgcgact cccgaggcgc gcctgccgcg
     2221 gctgaagcag gtgtttgaca cgattgagaa atggaccatg accaagacca agttcgaagc
     2281 catggaggag ctcaaccaac acgacatccc ctgcggcccc atcctctcga tggaggagct
     2341 ggcggcggag ccctcactgc gtgccaccgg caccgtggtc gaggtcgatc atccgaagcg
     2401 cggcaagtac ttgagcgtcg gtaaccccat caagatgtcc gacagcccga ccgaggtacg
     2461 gcgctcaccg cttctgggtg agcacacgaa cgaggtgctc aaagagctgg gactgagtgc
     2521 cccggacatc gatgcgttgc gcacggcgaa ggtcatatga gcggcgcgca cctcaaggac
     2581 atcgatgctg ctaacgatcc aggagaggct atggcctacg acaagacgac ggttcgcaga
     2641 gtactcgatc aggcaaagag cgccggccgc agctcactga cggcgcccga agccaaggag
     2701 atttgcgagg cctacggtat cgccatcccg agggaagccg tggcgacgac tgccacagag
     2761 gcagcgcggc tcgccagcag catcggcttt ccggtggtca tgaagatcgt ctcgccgcag
     2821 atcctgcaca agaccgaagc gggcggagtg gtggtcggcg tcaagagcgc agaggccgca
     2881 caggaagcct tcaatacgat tgtcgcgaat gcgcgccgct acaagtcgga tgcagcaatc
     2941 gatggcgtgc aggtgcagca gatgctcgcc ggcggccagg aagtgatcat cggcgcggtc
     3001 accgatcccg ctttcggcaa gctcattgcc ttcggcctcg gcggggttct ggtggaggtg
     3061 ctcaaggaca tcaccttccg cctggcaccg gcgatgcgtg cggacgcgct ctcgatgctc
     3121 gattccatcg ccgcggcgga gatcctgcgc ggggtgcgcg gcagcgaagc ggtgaaccgc
     3181 gaagcgctcg cgacgctcat agtgaacgtc tcgcagctgg tcaccgattt tccggagatc
     3241 tccgagctcg atttgaatcc ggtgttcgcg actgccaagg gcgcg
//
LOCUS       DATDMP010000103         2905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_795030, whole genome shotgun sequence.
ACCESSION   DATDMP010000103 DATDMP010000000
VERSION     DATDMP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795030"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VI195_01760"
     CDS             complement(<1..633)
                     /locus_tag="VI195_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823137.1"
                     /translation="MFSARRPTAALLIALAFEPAGAAPSPQAVIDPQAERAQVSEWRA
                     RRVEALTSDSGWLTLTGLFWLKEGENSFGRAPDNALVLAHPALAEHCGTFSVSGQKVR
                     FTAAAGSGVTTSGRTVSSLELSADSAGNPTVLASGTLRFYLIERAGNLGVRVRDLNNP
                     HRTGFRGFSYFPVSTDWVFDARFEPYRPARRITIINILGMQQEYASPGALV"
     gene            complement(674..2221)
                     /locus_tag="VI195_01765"
     CDS             complement(674..2221)
                     /locus_tag="VI195_01765"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HEY6823138.1"
                     /translation="MAELLRKGHFLGAKLRSIRRRNGLTLEELSVRAIQQQPDIAPSV
                     SYLSMIESGKRMPSPQLLALLADIFQRPTGWFLDENAELDPPAPLAGGPGARIPIEPA
                     FLFSKSLLQMAIPELLAQTGTTGRQFAHLLIRSHQEMSRNDFPDLERAADRIGERRFP
                     LSVEDLMRLARRHGLEIRWFDRKPVLARDKDREVRSMVRSFFEAPRIVYANRALQSDA
                     ARLKFDLAAHLGHKVLHGGDGLKSAHATGGEMGGSPEGGSGAAGMNAQDVLHAWRDFE
                     CSFFAGALLAPRVPFRRFLARERYRIEAGARLELTPAVVMRRMTKVSPYPYWHFFDAY
                     PPGYLRAVYRGNGIPLPWGNLAQVSDPCPNWAVFRMIDGAHGASGSQISVLRDGERSL
                     LYCCHSIRVHDMAGNPHVLSVGVDLAPALASNGIESDTVIESIFRECLHRRGEARIPK
                     AASQALGTIANVLNIAWIGDALASPARIICPRSNRCPRPERCEGARPSRAREIADVRE
                     EILGRES"
     gene            2331..>2905
                     /locus_tag="VI195_01770"
     CDS             2331..>2905
                     /locus_tag="VI195_01770"
                     /inference="COORDINATES: protein motif:HMM:NF045177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823139.1"
                     /translation="MNAETHGRVHLREQPGPRVEILGGKVERADEVLTPLALQLLGSL
                     HRRFNGRRLELLEARTRRQAEFDAGALPEFPAATAALRAAEWRVAPVPADLSNRRVEI
                     TGPVDRKMIINALNAPVQAFMADFEDANAPTWANCVGGQANLMDAVRRTITYVAPETG
                     KHYSLGSQVATLLVRPRGFHLLEKHCLVDERP"
BASE COUNT          477 a          966 c         1023 g          439 t
ORIGIN      
        1 caccagcgcg ccgggcgaag cgtactcctg ttgcatgccg aggatgttga tgatggtgat
       61 gcgacgcgcc ggccggtagg gctcgaagcg cgcatcgaac acccagtcgg tgctcaccgg
      121 gaagtagctg aaaccccgga agccggtgcg gtgggggttg ttcaggtcgc gtacgcgcac
      181 cccgagattg ccggcccgct cgatgaggta gaagcgcagc gtcccgctcg cgagcacggt
      241 cggatttccg gcgctgtcgg cgctcagttc gagcgagctc accgtccggc ccgaggtggt
      301 gacaccgctg cccgctgcgg ccgtgaagcg caccttctgt ccgctcacac tgaaggtgcc
      361 gcaatgctcg gcgagcgccg ggtgcgcgag caccagcgcg ttgtcgggcg cacggccgaa
      421 gctgttctcg ccctccttca accagaaaag gccggtgagc gtcagccatc cgctgtcgct
      481 cgtgagtgcc tcgacccgcc gggcgcgcca ctcgctgacc tgcgcacgct cggcctgtgg
      541 atcgatgacg gcctgcgggc tgggcgcggc cccggcaggc tcgaaggcga gcgcgatcaa
      601 cagggcggcg gtcgggcggc gggctgaaaa catggcatct ccatgggcat gcgagaccgc
      661 tgcaatgatg ccatcagctt tcgcgtccga ggatctcctc gcgcacatcg gcgatctcgc
      721 gcgcccgtga ggggcgggcg ccctcgcagc gctccgggcg tgggcagcgg ttgctgcgcg
      781 gacagatgat gcgcgccggg gaggcgagcg catcgccgat ccacgcgatg ttcagcacgt
      841 tggcgatcgt ccccaacgcc tggctggcgg ccttgggtat gcgcgcctcg ccgcgccggt
      901 gcaggcattc gcggaagatc gactcgatca ccgtgtcgct ctcgatgccg ttggaggcga
      961 gtgccggcgc gaggtcgact cccaccgaca gcacgtgcgg gttgccggcc atgtcgtgca
     1021 cacggatcga gtggcagcag tagagcagcg agcgctcgcc atcgcgcagc accgagatct
     1081 gcgagccgct cgcgccatgc gcaccgtcga tcatgcggaa caccgcccag ttggggcacg
     1141 gatcgctcac ctgcgcgaga ttgccccacg ggagtggaat gccgttgccg cggtagaccg
     1201 cgcgcagata gccgggcggg taggcatcga agaagtgcca gtaaggatag ggcgacacct
     1261 tggtcatgcg gcgcatcact acggcgggtg tgagctcgag ccgcgcaccc gcctcgatgc
     1321 ggtaacgctc gcgcgccagg aagcggcgga acggcacgcg cggcgccagc agcgcaccgg
     1381 cgaagaagct gcactcgaag tcgcgccagg cgtgcagcac gtcctgtgca ttcatgccgg
     1441 cggcgcccga gccgccctcg ggactgccgc ccatctcgcc gccggtggcg tgtgcggact
     1501 tgaggccatc gccgccgtgc agcaccttgt ggccgagatg cgcggcgaga tcgaacttca
     1561 ggcgcgccgc atccgactgc agcgcgcgat tggcgtacac gatgcgcggt gcctcgaaaa
     1621 aggagcgcac catgctgcgc acctcgcggt ccttgtcgcg cgccagcacg ggcttgcggt
     1681 cgaaccagcg gatctcgagc ccgtgccgcc gcgcgagccg catcagatcc tccaccgaga
     1741 gcgggaagcg gcgctcgccg atgcggtcgg cggcgcgctc gagatcggga aaatcgttgc
     1801 gcgacatctc ctggtgcgag cggatgagca ggtgcgcgaa ctgccgtccg gtggtgccgg
     1861 tctgcgcgag cagctcgggg atcgccatct gcagcaggct cttggagaac agaaacgccg
     1921 gctcgatcgg aatgcgcgcg cccggaccgc ccgcgagcgg cgccggcgga tcgagctcgg
     1981 cgttctcatc gagaaaccac ccggtcggcc gctggaatat gtccgcgagc agcgcgagca
     2041 gctgcggcga gggcatgcgc ttgccgctct cgatcatact caggtacgag accgagggag
     2101 cgatgtcggg ctgctgctga atggcgcgca ccgagagctc ctcgagcgtg agcccgttgc
     2161 gccggcgtat cgagcgcagc ttggcaccga gaaaatgacc tttgcgcaga agctccgcca
     2221 tatgttcagt ccacgtccgt gctgtcccag gggccgccag tttgtgaaat taacagtgtg
     2281 aagttttcac agtcaaattc gcgcgacgat agcgggtatg ctggcggggg atgaatgccg
     2341 agacacacgg acgggtgcat ctgcgcgagc aacccgggcc cagggtcgag atcctgggcg
     2401 gcaaagtcga gcgtgccgat gaggtgttga caccgctcgc gctgcagctg ctcggcagcc
     2461 tccatcggcg cttcaacggc cgccgcctcg agctgctcga ggcgcgtacc cggcgccagg
     2521 ccgaattcga tgccggcgcg ttaccggaat ttccggccgc gacggccgcg ctgcgcgccg
     2581 cggagtggcg cgtggcgccc gtgccggcgg acctcagcaa ccggcgcgtg gagatcaccg
     2641 gtcccgtcga ccgcaagatg atcatcaacg cgctcaacgc gccggtgcag gcgttcatgg
     2701 ccgacttcga ggatgccaat gccccgacct gggccaactg cgtcggcggc caggcgaact
     2761 tgatggacgc ggtgcggcgc acgattacgt acgtcgcgcc agaaaccggc aagcactact
     2821 cgctcgggtc acaggttgcc acgctgctcg tccggccgcg tgggttccac ctgctcgaaa
     2881 aacattgcct cgtcgacgag cgccc
//
LOCUS       DATDMP010000104         3431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_905936, whole genome shotgun sequence.
ACCESSION   DATDMP010000104 DATDMP010000000
VERSION     DATDMP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905936"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VI195_01775"
     CDS             complement(<1..771)
                     /locus_tag="VI195_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY6823140.1"
                     /translation="MAALGGQGGGVLADWLIAIAEANGYIVQSTSVPGVAQRTGATIY
                     YLEFFARTEAARTGREPVMALMPVPGDVDCVVASELAEAGRAIQRGLVSAQRTTLISS
                     THRAYAIGEKSAMGEGAPGTAELLELVRSQGKRTILFDMEAVAERHHSVVSAVLLGAI
                     SGAGVLPFPPASFEAAIREGGLAVKTNLAAFADAYALAERGGADPVDAAPRRAQLPPV
                     PARAQTPALQALLERIHRLPQSVQPFALEGTRRCLDYQD"
     gene            complement(807..2951)
                     /locus_tag="VI195_01780"
     CDS             complement(807..2951)
                     /locus_tag="VI195_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEY6823141.1"
                     /translation="MAERSFAREVQSLRIGAGEEFRGEGILAVTKALLQAGVAYVGGY
                     QGAPISHLMDVLADAEDILDELGVHFEASASEATAAAMLAASINYPLRGAVTWKSTVG
                     TNVASDALSNLASAGVRGGALIILGEDYGEGASIMQERTHAFAMKSQMWLLDPRPHLP
                     EIVRAVEQGFELSEASHTPVMLMLRIRACHVYGRFTAQANRRPAFSRADALANPTRDY
                     GRIILPPSTYAQEREKIEQRLPAAQRFIAERGLNEVMAGAGGAIGIVLQGGLYNNTLR
                     ALELLGRADAFGTTDLPLYILNVTYPLVPEEWERFCRDKTAVLIIEEGQPEYIEQAAN
                     QIVHQRGLATRIVGKEVLPMHGEYTVDVLRTGLKDFLERWSPRSAEPLERDMTREAPL
                     RPMGRLAALVPPRPSGLCTGCPERPFFSAMKLLQKETGPLHISADIGCHSFATLPPFN
                     MGNSIMGYGLGAAAASAFGSPPGKRAVSVMGDGGFWHNGLTSGIGNSVFNKHDDVTII
                     IDNGYSAATGGQDIPSSRAANEHRQGNNSIVDAVRGVGIKWVKRTHTYNMRETLRILR
                     EALNSDYRGPKVIVAEGECQLNRQRRVRPLTRKAIEAGERVVRERFGVDSDTCTGDHS
                     CIRLSGCPSLTVKENPDPLRPDPVAYVDNSCVGCGVCGEVADAAVLCPSFYRADLLYN
                     PGRWDRFIHRLRAALIGWLQNGSERRQLQRAL"
     gene            3061..>3431
                     /locus_tag="VI195_01785"
     CDS             3061..>3431
                     /locus_tag="VI195_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY6823142.1"
                     /translation="MRRIVCVGGGPAGLYAAILLRKALPQARIEVYERNRPDDTFGWG
                     VVFSDQTMTNFRDADPESHAAILSSFHHWDEIAIHFNGRRMVSSGHGFCGIARKRLLG
                     LLQERAHALGVQQVFQREIED"
BASE COUNT          599 a         1144 c         1114 g          574 t
ORIGIN      
        1 atcctggtaa tcgagacaac ggcgcgtccc ttcgagcgca aacggctgta ccgattgcgg
       61 cagtcgatga atgcgttcca ggagtgcctg caacgcgggc gtctgcgcac gcgcgggcac
      121 ggggggtagt tgcgcccggc gcggcgccgc gtccaccgga tctgcgccac cccgctcggc
      181 gagtgcgtag gcatccgcga aggccgcaag attggtcttt acggcgaggc caccctcgcg
      241 gattgccgcc tcgaaggaag cgggcggaaa gggcagcact ccggcgccgg agatggcgcc
      301 aagcagtacg gcgctgacga cgctgtgatg gcgctcggcg acggcctcca tatcaaaaag
      361 gatggtgcgt ttcccctgcg aacgcaccag ctccagcagc tccgcggtgc ctggcgcccc
      421 ctcgcccatc gcgctcttct ccccgatcgc gtaggcacgg tgcgtcgagc tgatgagcgt
      481 ggtgcgctgc gcgctcacca ggccccgttg gatagcccgc ccggcctccg ccagctcgga
      541 ggcgaccacg cagtcgacgt cacccggaac gggcatcagg gccatcaccg gctcacggcc
      601 ggtgcgcgcg gcttcggtgc gcgcgaagaa ctccagatag tagatcgtgg cgccggtacg
      661 ctgcgccaca ccgggaaccg aggtggattg aacgatgtat ccgttcgcct ccgcgatggc
      721 aatcagccaa tcggcgagca cgcccccacc ctgcccgccg agcgccgcca cagtcagttt
      781 aatagtgcgc ggggccacgc tccgtcctac agcgctcgtt gtagctggcg acgctcgctg
      841 ccgttctgca gccagccgat caacgctgcc cggaggcggt gaatgaatcg gtcccagcgg
      901 cccgggttat agagcaggtc cgcacgatag aaggacgggc acaacaccgc cgcgtcggcg
      961 acctcgccac ataccccaca ccccacacag ctgttgtcga cgtatgcgac cggatcaggg
     1021 cgcaggggat ccgggttttc cttgacggtg agcgagggac agccggagag ccggatacac
     1081 gagtgatcgc cggtgcaggt gtcggaatcg acgccgaacc gttcgcgcac cacgcgctcg
     1141 cccgcctcga tcgccttgcg cgtcagcggg cgcacgcgcc gctggcgatt gagttggcac
     1201 tcaccctcgg cgacgatgac cttgggccca cggtaatcgg agttcagcgc ctcacgcaag
     1261 atcctcaagg tctcgcgcat gttgtaggtg tgcgtgcgct tcacccactt gatgccgacg
     1321 ccgcgcacgg catcgacaat cgagttattg ccctggcgat gttcattcgc cgcgcgtgaa
     1381 gaggggatat cctgaccgcc ggtggcggca gagtagccat tgtcgatgat gatggtgaca
     1441 tcgtcgtgct tgttgaagac cgagttcccg atgccgctcg tcaggccgtt gtgccagaag
     1501 ccgccatcgc ccatcacgga cacggcgcgc ttacccggcg ggctgccgaa agcggaggcc
     1561 gccgcggcgc cgagaccata gcccatgatg ctgttgccca tattgaaggg tggcagcgtg
     1621 gcgaaggagt ggcagccgat gtccgcgctg atgtgcagtg gaccggtctc cttctgcagc
     1681 agcttcatgg ctgagaagaa tggccgctcc gggcaaccgg tacagagtcc cgacggacgc
     1741 ggaggcacga gcgccgccaa ccgccccata ggccgcagcg gcgcctcgcg cgtcatgtct
     1801 ctctcgagcg gctccgcact ccgcggcgac cagcgctcga ggaaatcctt gagacccgtg
     1861 cgcaacacgt cgacggtgta ctcgccatgc atcggcagaa cctccttgcc gacgatgcgc
     1921 gtggcgagtc cgcgctggtg cacgatctga tttgccgcct gttcgatata ttccggctgg
     1981 ccttcctcga tgatgagcac cgccgtcttg tcgcggcaga agcgctccca ctcctcgggc
     2041 accaggggat aggtcacgtt gagtatgtag agcggcagat ccgttgtgcc gaaggcgtcg
     2101 gcacgtccga gcagctcgag agcccgcaaa gtattgttgt acagcccgcc ctgtaacacg
     2161 atgccgatcg cgccgccggc gcccgccatc acctcgttca gcccgcgctc ggcgatgaat
     2221 cgctgcgcgg ctggcaggcg ctgctcgatc ttctcgcgct cttgcgcata ggtcgacggc
     2281 ggcaggatga tccggccgta gtcgcgcgtt ggattcgcca gggcatccgc gcggctgaat
     2341 gccggtcggc ggttggcctg cgccgtgaag cggccgtaca cgtgacaggc acggatacgc
     2401 agcatcagca tcaccggcgt gtggctggcc tccgagagct cgaacccctg ctccaccgcg
     2461 cgcacgatct cgggcagatg gggacgcgga tcgagcagcc acatctgcga cttcatggcg
     2521 aaagcatgag tgcgctcctg catgatgctc gcgccctcgc catagtcctc gccgaggatg
     2581 atgagggcgc cgccgcgcac gcccgccgag gcgagattcg agagtgcatc ggaagcgaca
     2641 ttggtaccga ctgtcgactt ccacgttacc gccccgcgca gggggtagtt gatggaggcc
     2701 gcaagcatcg cagcggcagt cgcctccgag gcgctcgcct cgaagtgcac gccgagctca
     2761 tcgagaatgt cctcggcgtc ggccagtacg tccatcagat gcgagatcgg tgcaccctgg
     2821 tagccaccca cataggcgac cccggcctgc agaagcgcct tggtcactgc caggatgcct
     2881 tcgccgcgga actcctcgcc ggcgccgatc cgcagcgact ggacttccct ggcgaacgag
     2941 cgttcagcca cgaacccctc ctactttatc ggcccgaata atagttgaca cttgaagtaa
     3001 ttgcaaatat aacgtacccc gcccaggcgg agtaggggcg ccacaggaga tccggcgggt
     3061 atgagacgaa ttgtctgcgt gggtggcggc ccggcgggtc tgtacgcggc gattctgttg
     3121 cgcaaggcgc tcccccaggc gcgcatcgag gtctacgagc gcaaccgccc ggacgacacc
     3181 ttcggctggg gcgtcgtgtt ctccgaccag accatgacca atttccggga cgccgacccc
     3241 gaatcgcacg ctgccatcct ttcgagcttc catcactggg acgagattgc cattcatttt
     3301 aacggcaggc gaatggtcag cagcgggcat ggcttttgcg gcattgcccg caagcgcctg
     3361 ctcgggctgc tgcaggagcg cgcgcatgcc ctcggcgtgc agcaggtatt ccagcgcgag
     3421 atcgaggacg a
//
LOCUS       DATDMP010000105         3510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1461048, whole genome shotgun sequence.
ACCESSION   DATDMP010000105 DATDMP010000000
VERSION     DATDMP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461048"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..2469)
                     /locus_tag="VI195_01790"
     CDS             complement(169..2469)
                     /locus_tag="VI195_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6823143.1"
                     /translation="MSIAGLLSLLFALIAVLLLVLYALQRRELHKLAELSQQLQRIVI
                     GGLPARVEMSSDKPEVAALITAVNHLLTRGTTGNERDAAYAPKLFTELGERIHEAVLV
                     HGDVIVYANRQFASFVGVDRAELIGRRLADLVPPEYSELVSENIRRRLAGESAAERYE
                     IDMVGLQGQVTRLEIASSPVDYNQGNALLITGVEILPTMTAQALRLPLEAASEPQSLA
                     LNSLAEAIIATDKDGHISYMNPAAEQLTGSAADAATGKLLEEIVSLVDETDRRLLSDP
                     VHQALTTGAAVNLSRRALMLSRTNGSERSIELSASPIRNAARELTGAVVMLHDVTEMR
                     GLTRQMSYQAAHDALTGLVNRREFERRLEEAIESGHRGDGQHVLCYLDLDRFKLVNDT
                     SGHLAGDSMLREVAKLLRDAVRDSDTVGRLGGDEFGILLVGCPLEKARQIADDVTRAV
                     GDHRFVWRDKIFNIGASIGLVEISRESGTLEELLAAADTACYVAKKQGTGRVVVYSAR
                     DEALARHTGEIQWLQRLQSALKENRFHLYQQVIVPAHGDTSGPAMEVLVRLHDESGHD
                     LPPAEFMRAAERYRLMGLVDRWVVQTTLAALGRGAIVVPPEHSVAINISGQTLGDLQF
                     LEFVVECLDSTGVAPGQVCFEIGESAVIANLDHARRFVGVLHGMGCQFALDDFGSGVG
                     SFSNLKTLPLDYLKIDGSFIRNLARDSVNQAMVTAMIKLARTLNFKVIAEQVEDSAAE
                     EAARRMGVDYLQGYAIGRPQPLELAA"
     gene            2708..3355
                     /locus_tag="VI195_01795"
     CDS             2708..3355
                     /locus_tag="VI195_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY6823144.1"
                     /translation="MVVDAREQMIEQQVRAWDVLDERVLAVMRRVPRELFVPQGQRYR
                     AYADAEVPLPRGQHMLRPSVVGRLLQALLPLPGERVLEIGAGSGFVTACLRTMATHVR
                     ALEIFPELADAASRNLALFGLRDVEVIAADAMQGDGGARYDVVALTASLPVYDARFER
                     MLAPGGRLFVVVGKPPVMDARLVRRTSADSVTSRSLFETVIDPLVNAVRPPEFTF"
BASE COUNT          554 a         1161 c         1166 g          629 t
ORIGIN      
        1 tcaagatcat caacatcgtc gcgctgctgc tggtgccgtt gctgtaaaag acgtcgctct
       61 tgccggctgc ctgcggcgca ggcgcacact gttttcccga atagttaacc aaaaggtgaa
      121 ctaacgcagg gaaccctgta ctgactgccc gggcgcggct cgctatggtt acgccgccag
      181 ctccagcggc tgcgggcggc cgatggcata gccctgcagg taatcgacgc ccatgcggcg
      241 cgccgcctct tccgcggcgc tgtcctcgac ctgctccgcg atcaccttga aattgagcgt
      301 gcgtgcgagc ttgatcatcg ccgttaccat cgcctgattg acgctgtcgc gcgcgaggtt
      361 gcggatgaac gagccgtcga tcttgaggta atcgagcggc agcgtcttga ggttggagaa
      421 cgagccgaca cccgagccga agtcatcgag cgcgaactga cagcccatgc cgtgcagcac
      481 gccgacgaag cggcgcgcgt ggtcgaggtt ggcgatgacg gcgctctcgc cgatctcgaa
      541 gcacacctgg ccgggggcga ctccggtgct gtcgagacac tccaccacga actcgagaaa
      601 ctgcaggtca ccgagtgtct gtccggagat gttgatggcg acgctgtgct cgggcggcac
      661 cacgatggcg ccgcggccga gcgcggcgag cgtggtctgt accacccagc gatccaccag
      721 gcccatgagc cggtagcgct cggcggcgcg catgaactcc gccggcggca gatcgtgtcc
      781 ggactcgtcg tgcagccgca ccagcacctc catcgccggg ccgctcgtat cgccatgggc
      841 tggcacgatc acctgctgat agaggtggaa gcggttctcc ttgagtgcgc tctgcagccg
      901 ctgcagccac tggatctcgc cggtatggcg cgccagcgct tcatcgcgtg ccgagtaaac
      961 caccactctg cccgtgccct gcttcttggc gacatagcag gccgtgtcgg ccgcggcgag
     1021 taactcttcc agcgtgccac tctcgcgcga gatctccacc agtccgatgg aagcgccgat
     1081 gttgaagatc ttgtcgcgcc atacgaagcg atggtcgcca accgcgcggg tcacgtcatc
     1141 ggcgatctgc cgcgccttct ccagcggaca gcccaccagc aggatgccga attcatcgcc
     1201 gccgagccgg ccgacggtgt cgctgtcgcg caccgcatcg cgcagcagct tcgccacctc
     1261 gcgcagcatg ctgtcgccgg ccaggtgacc gctggtatcg ttcaccagtt tgaagcgatc
     1321 gagatccagg tagcacagca cgtgctgccc atcgccgcga tgtccgctct cgatggcctc
     1381 ttccagacgc cgctcgaact cgcggcggtt gacgagcccg gtgagcgcat cgtgggccgc
     1441 ctggtaggac atctgccgcg tgagcccgcg catctcggtg acatcgtgca gcatcaccac
     1501 cgcaccggtg agctcgcgcg cggcgttacg gatcggtgac gccgacagct cgatcgaacg
     1561 ctcgctgcca ttggtgcgcg acagcatcag cgcgcgccgg ctgaggttca ccgccgcgcc
     1621 ggtggtgagc gcctggtgca cgggatccga cagcagccgg cggtcggtct catcgaccag
     1681 gctgacgatc tcctcgagca gcttgccggt cgccgcgtcg gccgcgctgc cggtgagctg
     1741 ctccgccgcc gggttcatgt agctgatgtg gccgtccttg tcggtggcga tgatggcttc
     1801 ggcgagcgag ttgagcgcca gtgactgcgg ctcggaggcg gcttccagcg gcagacgcag
     1861 tgcctgcgcc gtcatcgtgg gaaggatctc gaccccggtg atgagcagcg cgttgccctg
     1921 gttgtaatcg accggagatg aggcgatctc gagccgggtc acctgtccct ggagacccac
     1981 catgtcgatc tcgtaccgct ccgcggcgct ctctcccgcg agacgccgcc ggatgttctc
     2041 gctcaccagc tcggagtact cgggtggcac gaggtcggcg agacgccgtc cgatcagctc
     2101 ggcgcgatcc acgccgacga agctggcaaa ttggcggttc gcatatacga tcacatcgcc
     2161 gtgcaccagc accgcctcgt gaatgcgctc gccgagctcg gtgaacagct tgggcgcgta
     2221 cgcggcatcg cgctcgttgc ccgtggtgcc gcgcgtcagc aggtgattga ccgcggtgat
     2281 gagtgccgcc acctcgggct tgtcgctgga catctccacg cgggccggca ggccaccgat
     2341 gacgatgcgc tgcaactgct gcgacagctc ggcgagcttg tgcagctcgc ggcgctgcaa
     2401 cgcgtacagc acgagcagca ggacggcaat gagcgcgaac agaagggaca aaagcccggc
     2461 aatcgacatc tgcgttttct tctcaagcct ttcgcttggg ttcccgtggg ctccgcaggc
     2521 gcttctgatt cgacttgtcg cgccaggggc ttattggcag ggcaagcata tcccagcagg
     2581 gggcacgccc cgtcggtatt tcaccatatc tcgtcgcagg gcggcttggc agcgagcggg
     2641 aaaaccctta acttcaccgg ccttttcagg tgtggcaata agtccaattc tggaggccgg
     2701 cgtgcagatg gtggtggatg cccgcgagca gatgatcgag cagcaggtgc gtgcctggga
     2761 cgtgctcgat gagcgcgtgc tggcggtcat gcggagggta ccacgcgagc tgttcgtccc
     2821 ccagggacag cgttaccgcg cctacgccga cgcggaggtg ccgctgccac gcggtcagca
     2881 tatgttgcgt ccgagtgtgg tcggccggct gctgcaggcg ctgctgccgc ttccggggga
     2941 gcgggtgctg gagatcggtg ccggtagcgg gttcgtgact gcctgcctgc gcacgatggc
     3001 gacgcacgtg cgtgcgctcg agatatttcc cgagctggcc gatgcggcga gccgcaacct
     3061 cgcgctcttc gggctgcgcg atgtcgaggt gatcgccgcc gatgccatgc agggcgatgg
     3121 tggcgcccgc tacgacgtgg tcgccctgac cgcgtcgctc ccggtctacg atgcgcgctt
     3181 cgagcgcatg ctcgcccccg gcggccggct gttcgtcgtc gtgggcaagc cgccggtaat
     3241 ggacgcgcgg ctggtgcggc ggacctccgc agactcggtg accagccgca gcctgttcga
     3301 gaccgtcatc gatccgctgg tgaatgcggt gcgaccgccg gaattcacgt tctgaccagc
     3361 cggctcagcc cggtcactga ccaaagggag atgccatgaa tcgcgccgcg actctcgctc
     3421 tcgtattgct tgcgcttccg ctcgccgcgc ccgccaagga cctgatcggc gtgttcgagg
     3481 atgcgctgca caacgatccg gtcatccgtc
//
LOCUS       DATDMP010000106         2763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_683764, whole genome shotgun sequence.
ACCESSION   DATDMP010000106 DATDMP010000000
VERSION     DATDMP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683764"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VI195_01800"
     CDS             complement(<1..756)
                     /locus_tag="VI195_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY6823145.1"
                     /translation="MPARAAACSAVLLALGLLATAQAAAPPHTPARQVAREVLPPIRH
                     VFVLLLENQSYHVTFGEGSPASYLARVLPARGALLTHYYAIGHASLGNYIALVSGQAP
                     NLATQLDCPTYEDFRPRTPQLDEHGQLPGSGCVYPRIVRSLPDQLESAGFSWRAYMED
                     MGKNPARESSTCGHVRLGAPETTNSAAAGDQYAAKHNPFVYFHAIIDDQARCDTHVVN
                     LERLPQDLASVATTANYIFITPNLCSDGHDVPCI"
     gene            complement(757..2013)
                     /locus_tag="VI195_01805"
     CDS             complement(757..2013)
                     /locus_tag="VI195_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY6823146.1"
                     /translation="MRKAGGGALLFIFILVLVDSIGFGIILPVLPRLIMQLTGVGIDR
                     AARYGGWLSFVYALAQFFCAPVLGNLSDRFGRRPVLLFAVLALGVDYLIMGFAPVISW
                     LFLGRLIAGIAGASFTPAYAYVADITPAQRRAQNFGLMSAAFGIGFILGPAIGGLLGT
                     FGPRAPFFTAGIIALANAALGYSALPESLPRDKRREFHWLRANPLGTLMQMRRYPAVL
                     LALGALFLWQLAHQVLPGTWAFYTISKFHWSSAQVGGSLAFVGLLMALSQGVLTRVLI
                     PWLGGERRAALAGMLAALLAYIGYAFATQGWMMYVVGLTTFLFALTYPSMNALASQQI
                     PANAQGELQGAVASLGSLSAIVGPPLMTQIFGHFSDPTASVYFPGAAFLTAAALTVLC
                     AMLFLRVLRLTPQLVTAHERRAASEA"
     gene            complement(2099..>2763)
                     /locus_tag="VI195_01810"
     CDS             complement(2099..>2763)
                     /locus_tag="VI195_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6823147.1"
                     /translation="AVAAPAPQAEVVVNNWFNPNLDYRWYMLPNLVGMLSLSVALMIT
                     ALSVARERELGTFDQTLVSPATPVEIALGKLLPPLVVAAVQTTLYILIVTLLYRVPFR
                     GNLLVFYAAVLAFAAACAGVGLCVSSLVRTQQQAFLGAFALLLPFSLLSGFATPVENM
                     PWWLQFITSMNPLAHMLRLMQGLFLKGASLASMTLELERLLEIALCTTCAAVLLFSRK
                     AA"
BASE COUNT          544 a          863 c          987 g          369 t
ORIGIN      
        1 gatgcagggc acatcgtggc cgtcgctgca gaggttgggg gtgatgaaga tgtagttggc
       61 ggtggtggcg acgctggcga gatcctgtgg caggcgctcg aggttcacga cgtgcgtgtc
      121 gcagcgcgcc tggtcatcga tgatcgcatg gaagtaaacg aacggattgt gcttggcggc
      181 gtactgatcc cccgcggcgg cggagttggt ggtttcgggc gcgccgaggc gcacgtgccc
      241 gcaggtggat gactcgcgcg cggggttctt gcccatgtcc tccatgtacg cccgccagct
      301 gaaacctgcg gactcgagct ggtcgggaag cgagcgcacg atgcgcggat acacgcagcc
      361 cgatccgggc agctggccgt gctcatccag ctgcggcgtg cgcgggcgga aatcctcgta
      421 ggtggggcag tcgagttgcg tcgccagatt cggcgcctgg ccactcacca gggcgatgta
      481 attgccgaga ctcgcatgtc cgatcgcgta atagtgcgtg agcagtgcgc cgcgtgccgg
      541 cagcacgcgc gccaggtagg acgccggcga gccctccccg aaagtgacgt ggtaggactg
      601 attctcgagc agcagcacga agacatgacg gatgggtggc agcacctcgc gcgccacctg
      661 ccgcgcgggc gtgtgcggcg gtgccgcggc ttgggcagta gcgaggaggc cgagcgcgag
      721 cagcacagcg gagcacgcgg cggctcgcgc tggcattcac gcctcggacg cggcgcgccg
      781 ctcgtgcgca gtgacgagct gcggcgtcag ccgcagcacc cgcaggaaca gcatggcgca
      841 gagcaccgtc agcgccgccg cggtgagaaa cgcggcaccg gggaaataca cgctggccgt
      901 cgggtcggag aagtggccga agatctgcgt catcagcggc ggcccgacga tggcgctcaa
      961 actgccgagc gaggcgaccg caccctgcag ctcgccctgg gcgttcgctg ggatctgctg
     1021 cgaggcgagc gcattcatgg agggataggt gagcgcgaac aggaacgtgg tgagcccgac
     1081 cacgtacatc atccagccct gggtcgcgaa cgcgtagccg atgtaggcga gcagcgccgc
     1141 gagcatgccg gcgagcgccg cacgccgctc gcccccgagc catggaatca acacccgcgt
     1201 cagcaccccc tgcgacagcg ccatgagcag accgacgaag gcgagtgagc caccgacctg
     1261 cgcgctcgac cagtggaact tggagatggt gtagaaggcc caggtacccg gcagcacctg
     1321 gtgcgcgagc tgccacagga agagcgcccc gagggccagc agcaccgccg gatagcggcg
     1381 catctgcatg agcgtgccga gcggattggc gcgcagccag tggaactcgc ggcgcttgtc
     1441 gcgtggcagc gattcgggca atgcgctgta gccgagggcg gcgttggcga gcgcgatgat
     1501 gccggcagtg aagaacggcg cacgcggacc aaaggtaccg agcaacccgc cgatggccgg
     1561 tccgaggatg aagccgatgc cgaacgccgc actcatgagg ccgaagttct gggcacgccg
     1621 ctgcgccgga gttatgtccg caacataggc ataggccggc gtgaaggagg ccccggcaat
     1681 gccggcgatc agccgcccga ggaacagcca gctgatcacc ggagcaaagc ccatgatgag
     1741 gtagtcaacg cccagcgcca gcactgcgaa cagcagcacc ggccggcggc cgaaacgatc
     1801 gctgaggttg ccgagcaccg gcgcgcaaaa gaactgcgcc agtgcataaa cgaaagacag
     1861 ccagccaccg tagcgggccg cgcgatcgat gccgacaccg gtgagctgca tgatcagtcg
     1921 cggcagcacg ggcagaatga tgccgaagcc gatcgaatcg accagcacca ggatgaaaat
     1981 gaacagcagg gcgccgccgc cggctttgcg cacgttagtg agtccttcgc tcgtgggccc
     2041 tgcgtgaggg cgcgctatct tagccgaagc ggcgtgtgcg cgcggcgcac ccgggagctc
     2101 acgcggcctt tctgctgaac agcagcaccg cagcgcaggt cgtgcacagt gcgatctcga
     2161 gcaagcgctc gagctccagc gtcatggaag cgagcgatgc ccctttgaga aacagcccct
     2221 gcatcaggcg cagcatgtgg gcgagtggat tcatgctggt gatgaactgc agccaccagg
     2281 gcatgttctc caccggcgtc gcgaagcccg acagcagcga gaatggcagc aacagcgcaa
     2341 aggcaccgag gaaagcctgc tgctgcgtgc gcaccaggga cgagacgcac aggccgaccc
     2401 cggcgcaggc cgcggcaaat gccagcaccg cggcgtagaa caccaacagg ttgccgcgga
     2461 acggcacccg atagagcagg gtcacgatca ggatgtagag cgtggtctgc acggcggcca
     2521 ctaccagcgg cggcagcagt ttgccgaggg cgatctcgac gggcgtagcc ggcgacacca
     2581 gcgtctgatc gaaggtgccg agctcgcgtt cgcgcgcgac cgagagcgcg gtgatcatga
     2641 gcgcaacgct gaggctcagc ataccgacca gattcggcag catgtaccag cggtaatcga
     2701 gattcgggtt gaaccagttg ttgactacca cttcggcctg gggcgccggc gcggcaacag
     2761 cgc
//
LOCUS       DATDMP010000107         4146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2128493, whole genome shotgun sequence.
ACCESSION   DATDMP010000107 DATDMP010000000
VERSION     DATDMP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128493"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..808)
                     /locus_tag="VI195_01815"
     CDS             complement(74..808)
                     /locus_tag="VI195_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6823148.1"
                     /translation="MAENLLRLAALALLVAFLLSPQTFAPLFAPLTHYGAPPIYDQGN
                     LLMLALAQLGTVCCAAAASTLVAVGLGILVTRPAGAQFLPLSRTLVNIGQTFPPVAVL
                     AVAVPLVGFGEKPTLIALFAYGLLPIFENTIAGLRSCPPAVLEAASGMGMSARERLRS
                     VELPLAMPLILEGVRLSLVINVGTATIGSTVAAKGLGEVIIAGLLSSNTAFILQGGLV
                     TGLMAVLLYDGLATLERRLTRPVPLD"
     gene            complement(808..1740)
                     /locus_tag="VI195_01820"
     CDS             complement(808..1740)
                     /locus_tag="VI195_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6823149.1"
                     /translation="MIQIESLTKQYGSTTVVDQVSFNIERNSITVVVGTSGSGKSTLL
                     RMINRLVEPTAGRVLLDGADTRSEPAHLLRRRIGYAIQGHGLFPHWSVAENIATVPRL
                     LGWPDARVQARVTELLEVFQLDPALYAGAFPHQLSGGQQQRVGVARALAAEPAVLLMD
                     EPFGALDPIIRAKAQEDLLAIQRRFGTTMVLVTHDMDEAFHLGHRIAVMSEGRLLQYD
                     SPAVLLTHPADPFVARMTGLSDRAMKLLALTSAGQAALPGHSDGPRFASSASLREVLS
                     ELLWSGATSALVLGDDGTPRGQLTVSAILAHGRP"
     gene            complement(1737..2882)
                     /locus_tag="VI195_01825"
     CDS             complement(1737..2882)
                     /locus_tag="VI195_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6823150.1"
                     /translation="MLLGAVGLAALIGLPFIVFKSNRIMPGEARALQQVLPAWGALGL
                     QTVLLLTAAVALGVADAKRRLTAALAGLLVLALALATAANALTPPGDKVVRIAPGGAF
                     WVLLICLGLLATDAITRMRPRPLVRVLLLALSIAITAAALASGMFDNLSVLREYTVNS
                     GRFPLELWRHVWLAGGSLLAAVIVGLPLGILCHRAPRLRPPVLGTLNVIQTIPAIALF
                     GILMAPLGALAASFPAVAALGIRGIGAAPAVVALFLYSLLPIVANTVVGLRRVSQAAV
                     EAAAGMGMTPWQVLRAIELPLALPVILTGIRVVLVQNIGMVTIAALIGGGGLGTFVFQ
                     GIGQTAIDLVLLGAIPIVVLAFSAAVLLDALVEVLDRTSSIPGELSP"
     gene            complement(2949..3860)
                     /locus_tag="VI195_01830"
     CDS             complement(2949..3860)
                     /locus_tag="VI195_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY6823151.1"
                     /translation="MSRIVKSLCVVTLLALATAFPLEAAVVVSSKLSSESAMLGQMIR
                     LLLRAQGIPVVDRTRIGATPVVRQALLAGEIDLYVEYTGNAGLFFNDAADPAWKDLER
                     GYELGARLDYAANRIVWLTPARASNAWALAVRSDVAQAYHLRTMSDFGRWVAGGGDVT
                     LICSAEFANAGTLRSLERTYGFTLHSSQLIVLAGGETAATIAAAAAGTNGANTAMVYG
                     TDGGIAAAHLQILEDDRHDQPVYAPVPVIRESVLRQYPQIAAIVKPLMESFDRESLQR
                     LNARVQINGESDETVAVEYLKTKGWLK"
BASE COUNT          745 a         1408 c         1419 g          574 t
ORIGIN      
        1 caaacctcgg ccagcattgt acccgctcgg ggcacctccg cgccgaccaa gctatcgctt
       61 tgcgagagtg gtctcagtcg aggggcacgg gccgggtgag ccgccgttcg agggtggcga
      121 ggccgtcgta cagcagcacc gccatgagac cggtgaccag cccgccctgc aggatgaagg
      181 cggtattact tgacagcagc ccggcgatga tcacctcacc gagtcccttg gcggcgaccg
      241 tggagccgat ggtggccgtc ccgacattaa tgacgaggga cagccgcact ccttccagga
      301 tgagcggcat cgccagcggc aactctaccg agcgcagccg ctcgcgcgcg ctcatgccca
      361 tgccgctcgc cgcctcgagc accgccggcg ggcagctgcg cagtcccgcg atggtgttct
      421 cgaaaatagg cagcaacccg tacgcaaaga gcgcgatcag ggtgggtttc tccccgaagc
      481 cgaccagcgg cacagcgacc gcgagcaccg cgaccggggg aaaggtctgg ccgatattga
      541 cgagcgtgcg cgaaagcggc agaaactgcg ctccggccgg tcgggtcacg aggataccga
      601 gtcccaccgc cacgagcgtg ctcgccgccg cagcgcagca caccgtgcca agctgtgcga
      661 gggccagcat gagcagattc ccctggtcgt agatcggcgg cgccccgtag tgggtgagcg
      721 gcgcgaacag cggcgcgaag gtctgcggcg agagcaggaa agcgaccagg agcgcgagag
      781 cggccaggcg cagaagattc tctgccatca gggccgcccg tgcgccagta ttgcggacac
      841 cgtgagctgg ccgcggggtg tgccgtcatc gccgagcacg agcgccgatg tcgccccgct
      901 ccagagaagc tccgagagca cctcgcgaag cgatgcggat gaggcaaaac gcggtccatc
      961 ggagtgtccc ggaagtgccg cctgcccggc gctggtgagc gccagcagtt tcatggcccg
     1021 atcgctgagg cccgtcatgc gcgcaacgaa cggatccgcc gggtgagtca gcaggaccgc
     1081 cgggctgtcg tactgcagca gccgtccctc actcatgacc gcgatgcgat ggccgaggtg
     1141 gaacgcctcg tccatgtcgt gggtgacgag caccatggtc gtgccgaagc ggcgctggat
     1201 ggcgagcagg tcctcctgcg ccttggcacg gatgatcgga tcgagagccc cgaaaggctc
     1261 atccatcagt agcacggcgg gctcggccgc gagcgcgcgc gcgactccga cgcgctgctg
     1321 ctggccaccg gaaagctggt gcgggaaagc gcccgcatac agggcggggt cgagctgaaa
     1381 cacctcgagc agctcggtga cacgcgcctg gacgcgcgcg tccggccagc ccagcagccg
     1441 aggcacggtg gcgatgtttt ccgccacact ccagtggggg aaaagcccgt gaccctggat
     1501 cgcatagccg atacgccggc gcagcaggtg cgcaggctcg ctgcgcgtat cggcgccatc
     1561 gagcagcacg cggccggcgg tgggctcgac caggcggttg atcatccgca gcagcgtcga
     1621 tttgcctgag ccggaggtgc cgacgaccac ggtgatcgag ttgcgctcga tgttgaagga
     1681 gacctgatcg acgacggtag tcgagccgta ttgcttggtg aggctctcga tctggatcat
     1741 gggctgagtt cccccgggat ggaggacgtg cggtcgagta cctcgaccag cgcatcgagg
     1801 agtaccgctg cggagaaggc gagcaccacg atcggtatgg cgccgagcag caccagatcg
     1861 atggcagtct gtccgatgcc ctggaagacg aaggtgccga gcccgccgcc gccgatcagt
     1921 gcagcgatcg tcaccatgcc gatgttctgc acgagcacca cgcggatgcc ggtgagtatc
     1981 accggtagcg ccaggggcag ctctattgcg cgcagcacct gccagggcgt catacccatg
     2041 cccgccgccg cctcgacggc cgcctgcgac acgcgccgca atccgaccac cgtgttcgcg
     2101 acgatcggca gcagcgaata gagaaacaag gccaccactg ccggcgctgc accgatgccg
     2161 cggatgccga gcgcggcgac ggccgggaaa ctcgccgcca gcgccccgag cggtgccatc
     2221 agaatgccaa acagcgcgat cgccgggatg gtctgaatga cattcagcgt ccccaacacc
     2281 ggtggccgca ggcgcggagc ccggtggcag agaatgccga gcggcaatcc aacgatcact
     2341 gcggcaagca gcgagccacc ggcaagccat acgtggcgcc acagctcgag cggaaaccgc
     2401 ccggaattca cggtgtactc gcgcagcacc gaaagattgt cgaacatgcc gctcgcgagc
     2461 gcggcggctg tgatggcaat cgacagcgcg agcagcaaca cgcgcaccag cggccgcggc
     2521 cgcatgcgcg tgatggcatc ggtagccagc agtccgaggc atatgagcag cacccagaaa
     2581 gcccccccag gggcgatgcg caccaccttg tcgccgggtg gggtgagcgc attggccgcg
     2641 gtagcgagcg cgagcgcgag tacgagcaag ccggcgagcg cggcggtcag ccgccgcttc
     2701 gcatccgcga cgccgagcgc cactgccgcc gtgagcagca ggaccgtctg caggccgagc
     2761 gcgccccagg ccggcagcac ctgctgcagc gcgcgcgcct caccgggcat gatgcggttc
     2821 gatttgaaga ctatgaacgg cagcccgatg agcgcggcca ggcccaccgc accgagcagc
     2881 atccccagcc gatcgatgcg ctcgcgccgt ggcagagcga tggcgagtcg aagctccagt
     2941 gccgccccct acttcagcca gcccttggtt ttcagatact cgacggcgac cgtttcatcg
     3001 gactcgccat tgatctgcac gcgcgcattg aggcgctgca gggactcacg gtcgaagctc
     3061 tccatgagcg gcttgacgat cgcggcaatc tgcgggtact ggcgcaacac gctttcgcgg
     3121 atgaccggca ccggagcgta caccggctga tcgtgccgat cgtcctccag gatctgcaga
     3181 tgggcggcgg cgatgccgcc atcggtgccg tacaccatcg ccgtgttggc tccattggtg
     3241 ccggcggcgg ccgcggcgat ggtcgccgcc gtctcgccgc cggcgagcac gatcagctgc
     3301 gaggagtgca gcgtgaagcc gtaggtgcgc tcgagggaac gcagcgtgcc ggcgttggcg
     3361 aactccgccg agcagatgag cgtcacgtct ccgccgcccg cgacccagcg gccgaaatca
     3421 ctcatggtgc gcaggtgata ggcctgcgcc acatccgagc gtaccgcgag tgcccaggcg
     3481 ttgctggcgc gcgcgggagt tagccacacg atgcggttgg ccgcgtagtc gagccgcgca
     3541 ccgagctcgt agccgcgctc gagatccttc caggcggggt cggccgcatc gttgaagaac
     3601 agccccgcgt tacccgtata ctcgacgtac agatcgatct cgccggcgag cagtgcctgc
     3661 cgcaccacgg gcgtcgcgcc gatgcgagtg cggtcgacga ccggaatgcc ctgcgcacgc
     3721 agcagcagcc ggatcatctg gccgagcatc gccgactcgc tcgagagctt cgaggagacc
     3781 accaccgcgg cctcgagcgg gaatgcggta gcgagcgcca gcagcgtcac gacgcacagc
     3841 gacttcacga tcctcgacaa tgccgctcct cgtggctgcg cctgccctgc tcggcacgtt
     3901 gtcggttgtc acccggcgcg tcaagagccc gctgctgcac gcggcccgcg ccgcgatttc
     3961 ctgagttggc cgtgcgcctt atgatggcgg caagccatga atgtgccgca cccgcgtcga
     4021 cgcgcgccga tacgcaaacg ctccacccgc tactcgatct ggcggctgct ccgtgagggg
     4081 ctgcgcagtc agcgcggctg gccgcgcgcc tgggcgaaag cgagcccgcg cgcacgctac
     4141 cgcgtc
//
LOCUS       DATDMP010000108         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_573007, whole genome shotgun sequence.
ACCESSION   DATDMP010000108 DATDMP010000000
VERSION     DATDMP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573007"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /gene="rnpA"
                     /locus_tag="VI195_01835"
     CDS             complement(<1..225)
                     /gene="rnpA"
                     /locus_tag="VI195_01835"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY6823152.1"
                     /translation="MPERLTLPAQLRLRRKSDFDAAYARGRRLGDGFFTVIALANESG
                     VPRLGLAVALRAAGGAVARNRLRRLIRESFR"
     gene            complement(300..434)
                     /gene="rpmH"
                     /locus_tag="VI195_01840"
     CDS             complement(300..434)
                     /gene="rpmH"
                     /locus_tag="VI195_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY6823153.1"
                     /translation="MKRTYQPSKVRRKRTHGFRARMATKAGRRVLARRRAKGRRRLIP
                     "
     gene            618..1940
                     /gene="dnaA"
                     /locus_tag="VI195_01845"
     CDS             618..1940
                     /gene="dnaA"
                     /locus_tag="VI195_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY6823154.1"
                     /translation="MEASLWTRCMGALEAELPEQQFNTWVRPLQAMEGNGALKLLAPN
                     RFVVDWVNANLLPRIGELLRDESTGDAPIITVEIGSRSTPLRGAAAPHPPRTGGLVVG
                     ARLNPDFTFATFVEGKSNQLAKAAALQVAENPGRAYNPLFLYGGVGLGKTHLMHSIAH
                     LVKERDPEARIAYVHSERFVGDMVRALQHNSMNEFKTAYRSLDALLIDDIQFFAGKER
                     SQEEFFHTFNELLEGQHQVILSCDRYPKEVEGLEERLKSRFGWGLTVAIEPPELETCV
                     AILMTKAHATGVSLAEEVAFFIAKRIRSNVRELEGALRRVIANSRFTGHAITLEFAKE
                     ALKDLLSLQAKLVTVDNIQKTVADYYKVRMADLLSKRRSRSVARPRQVAMALAKELTS
                     HSLPEIGDAFGGRDHTTVLHACKRIKELRDSEQRMKEDYSNLLRTLAG"
BASE COUNT          395 a          701 c          699 g          360 t
ORIGIN      
        1 gcggaaggat tcgcggatca ggcggcgcag gcgattacgt gcgaccgcgc cgccggcggc
       61 gcgcagggcc accgcgaggc cgaggcgcgg aacgcccgat tcgttcgcca gcgcaatcac
      121 cgtgaagaat ccgtcaccca gacgccgccc gcgcgcatag gcggcgtcga agtcgctctt
      181 gcgccgcagc cgcagttgcg ccggcagagt gagccgctcc ggcatcgggc cccggatagc
      241 ttgagatcgg gtctgcggcg cgctggttcc cggcacgacg tacccggcct gctcgccctt
      301 caggggatca gccggcgccg acccttggcg cgtcgccggg caagaacccg gcggccggcc
      361 ttggtggcca tgcgcgcgcg gaacccgtgg gtgcgcttgc ggcgcacctt gctcggttga
      421 taagtacgct tcataccgcg ggctccgtcg caagaggtgg cgcgaaaaaa aagggcggca
      481 aggctaagcc gttgagcgcc aaggtgtcaa tatacgtgcc gcggctgccg agccttcagc
      541 gagcggctcg atcggtatag acttcgcggc ccttccctca ctcgttcacg agcatcaagg
      601 tcgcggaggc aacgcgcgtg gaagcgtcgc tttggactcg ctgcatggga gcgttggagg
      661 cggagcttcc cgagcagcag ttcaatacct gggtgcgtcc gctgcaggcc atggagggca
      721 atggcgcatt gaagctcctt gcccccaatc gcttcgtggt cgactgggtc aacgccaatc
      781 tgctgccgcg catcggggag ctgctgcgcg acgagagcac gggcgatgcc cccatcatca
      841 ccgtcgagat cggctcgcgc agcacgccgc tgcgcggcgc tgcagcgccg cacccgccgc
      901 gcaccggtgg cctcgtggtc ggcgcacggc tcaatccgga cttcactttc gcgaccttcg
      961 tcgagggcaa gagcaaccag cttgccaagg cggccgcgct gcaggtggcg gaaaatcccg
     1021 ggcgcgccta caacccgctg ttcctctacg gcggcgtcgg tctcggcaag acgcatctca
     1081 tgcactcgat cgctcacctc gtcaaggagc gcgacccgga ggcacgcatc gcctacgtgc
     1141 actccgagcg cttcgtcggc gacatggtgc gggcgctgca gcacaattcc atgaacgagt
     1201 tcaagaccgc gtatcgctcg cttgatgcgc tgctcatcga tgacattcag ttcttcgcgg
     1261 gcaaggagcg ctcgcaggag gagttcttcc acaccttcaa cgagctgctc gaaggccagc
     1321 accaggtgat cctgagctgc gaccgctacc cgaaggaggt cgagggtctc gaggagcgtc
     1381 tcaaatcgcg cttcggctgg gggctcaccg tcgccatcga gccgcccgag ctcgagacct
     1441 gcgtggcgat cctcatgacc aaggcgcatg ccacgggcgt cagcctggcg gaggaggtgg
     1501 ccttcttcat cgccaagcgc atccgctcga acgtgcgcga gctggaaggt gcgctgcgcc
     1561 gcgtgatcgc aaactcgcgt ttcacgggac atgcgatcac gctggaattc gccaaggaag
     1621 cgctcaagga cctgctgtca ctgcaggcga agctcgtcac cgtcgacaac atccagaaga
     1681 ccgtcgccga ctattacaag gtacgcatgg cggatctgct gtccaagcgc cgcagccgct
     1741 cggtagcacg gccgcggcag gtggcgatgg cgctcgccaa ggagctcacc agtcacagtc
     1801 tgccggagat cggcgatgcg ttcggcggcc gcgatcacac caccgtgctg catgcctgca
     1861 agcgcatcaa ggagctgcgc gacagcgagc agcgcatgaa ggaagactat tcgaacctgt
     1921 tgagaactct ggccggctga tggcacaggt ttgtgcgttt gctgtggaca ggtgtgggga
     1981 taaagttata cacagctcgt gcaccactct tcggccgtcc ttcccgggag ttgtgccgtg
     2041 ccgaagcgat gtgcaatagc ctgtgccggc acgcaaaggc caagtaatcc ccgttttcca
     2101 cagtctctac tgcagtaaca acaaagactt tccagccaca agatcttcaa gagag
//
LOCUS       DATDMP010000109         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2128525, whole genome shotgun sequence.
ACCESSION   DATDMP010000109 DATDMP010000000
VERSION     DATDMP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128525"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VI195_01850"
     CDS             complement(<1..511)
                     /locus_tag="VI195_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6823155.1"
                     /translation="MPDPASFSVVTALRNGGEVEIRALRPSDRSALLLAVAHASDRSL
                     YRRFFAVRREFSDAEVAAFVNVDFTLQVALVAVSRASGGEVIVAGARYIVVRPGLAEV
                     AFTVVDEFQGQGIASAMLRHLVELARAAGLTAFIAEVLPSNTAMLRVLERSGLPLQKK
                     REGDVLHLTL"
     gene            703..>2154
                     /locus_tag="VI195_01855"
     CDS             703..>2154
                     /locus_tag="VI195_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY6823156.1"
                     /translation="MSSRESSTATARTPLSSEELRLMDCWWRACNYLAVGMIYLRANP
                     LLREPLKPEHIKARLLGHWGTSPELSFIWTHLNRLIKRDDLDMIYIAGPGHGAPGVLG
                     PAYLEGTYSEVYPDKSEDLEGLQKFLMQFSFPGHIGSHCTPETPGSIHEGGELGYSLA
                     HAYGAALDNPNLIVACVVGDGEAETGPLATAWHSNKFLNPIRDGAVLPILNLNGYKIA
                     NPTVLARIPHEELEALFVGYGYKPYFVEGSDPAKMHQAMAATLDTVIGEIRALQKKAR
                     DSGKAERARWPMIVLRTPKGWTCPKEIGGHKVEGSWRAHQVPIADVRGNPANLKLLES
                     WMRSYQPEELFDANARLRPELRALAPTGPRRMSANAHANGGLLRRELKLPDFRDYAVK
                     VTKPGALLHENTRPLGEFLRDVMRNNMANFRVFGPDETASNRLQAIYEVSKKTWMAEM
                     LPEDADGGELSRDGRVMEMLSEHTLIGWLEGYLL"
BASE COUNT          401 a          714 c          699 g          340 t
ORIGIN      
        1 gcagcgtgag gtgcagcacg tcgccctcgc gctttttttg cagtggcagg ccgctgcgct
       61 ccaacacccg cagcatcgcg gtgttactgg ggagcacctc ggcgatgaag gccgtgagcc
      121 ctgccgcacg cgcaagctcc acgagatgcc gcagcatggc gctggctatc ccctggccct
      181 ggaattcatc gacgacggtg aaggcgacct cggccaggcc gggacgaacg acgatgtagc
      241 gcgcacccgc tacgatgact tcgcccccgc ttgcccgact gacggcgacg agtgcgacct
      301 ggagtgtgaa atcgacgttg acgaaggcag ccacttccgc gtcgctgaat tcgcgtcgga
      361 cggcgaagaa tcgccggtaa agcgaccgat cgctggcgtg cgccacggcg agcagcaacg
      421 cgctgcgatc gctcggcctc agcgcccgga tctcgacctc cccgccgttg cgcagcgctg
      481 tgactaccga gaagctcgcg ggatccggca tgtcgcgcat catagcgaga cgcagcgccc
      541 cgcggccgtt gcagggccgc aatggccgcg ctgacgctca ggtgtggcac caagtgctca
      601 tgccgaaaaa tgccgcaagg ggataggatc gcaacggcat cgcttcgccc ggatcgataa
      661 attcatgaca gccacgtgac agtgcccacg gagtaatcgc aaatgagcag ccgagagtct
      721 tccaccgcca cggcgcgcac tccgctctcg agcgaggagc tgcgcctgat ggattgctgg
      781 tggcgcgcct gtaactatct cgcagtcggc atgatttatc tgcgcgccaa tccgctgctg
      841 cgcgagccgc tcaagcccga gcacatcaag gcccgcctgc tcggccactg ggggacgagc
      901 ccggagttgt cgttcatctg gactcatctg aatcgcctca tcaagcggga tgatctcgac
      961 atgatctaca tcgccgggcc cggtcacggg gccccgggtg tactcggtcc cgcttatctc
     1021 gagggcacct actccgaggt atatccggac aagagcgagg acctcgaggg gctgcagaaa
     1081 ttcctcatgc agttctcctt ccccggacac atcggctccc actgcacgcc cgagactccg
     1141 gggtcgatcc acgaaggagg cgagctcggt tatagcctgg cgcacgcgta cggcgcggcg
     1201 ctcgacaacc cgaatctgat cgtcgcatgc gtcgtcggcg atggtgaggc ggagaccggg
     1261 ccgctggcga ccgcctggca ctcgaacaag ttcctcaatc ccatccgcga cggggcggta
     1321 ctgccgatcc tcaacctgaa cggctacaaa atcgccaacc caacggtgct cgcgcgtatt
     1381 ccgcacgagg agctcgaggc cctgttcgtc ggctacggct acaagccgta cttcgtcgag
     1441 ggctcggacc cggccaagat gcaccaggcc atggccgcga ccctcgatac ggtgatcggc
     1501 gagatccgtg cgctgcagaa gaaggcgcgc gacagcggca aggcggagcg cgcccgctgg
     1561 cccatgatcg tgctgcgtac gccgaagggc tggacctgtc cgaaggagat cggcggccac
     1621 aaggtcgagg gctcctggcg cgcgcaccag gtgccgatcg cggacgtacg cggcaatccc
     1681 gccaatctga agctgctcga gagctggatg cgcagctacc agccggagga gttgttcgac
     1741 gcgaacgcgc gcctgcggcc ggagctacgc gccctggcgc caaccggtcc gcgccgcatg
     1801 agcgccaatg cgcacgccaa tggcgggctg ctgcgccggg agctcaagct gcccgacttt
     1861 cgcgactacg cggtgaaggt caccaagccg ggcgcgttgc tgcacgagaa cacgcggccg
     1921 ctcggggaat tcctgcgcga tgtgatgcgc aacaacatgg cgaacttccg cgttttcggc
     1981 cccgatgaga ccgcctccaa tcgcttgcag gcgatctacg aggtgagcaa gaagacctgg
     2041 atggcggaga tgttgcccga ggacgccgat ggtggcgagc tctcccgcga cgggcgggtg
     2101 atggaaatgc tctccgagca caccctgatc ggctggctgg agggatacct cctc
//
LOCUS       DATDMP010000110         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1795131, whole genome shotgun sequence.
ACCESSION   DATDMP010000110 DATDMP010000000
VERSION     DATDMP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795131"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VI195_01860"
     CDS             <1..733
                     /locus_tag="VI195_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823157.1"
                     /translation="ASFRGAVMTMDNLGESASHIDLKESESPAVRLDELQVAAPAGAI
                     QLSERHVDLQPHERVLISAERGAARTLLFRAMIGLWPWGKGRITRPARAAIMFLPGRA
                     YVPPGSLRAALCYPCTTDEFDDAASARALTAVGLERLQPLLDRTERWDQRLNDDEKQC
                     LAIARVILQRPRWVVLDGALELLDGASRQRVKALFTSELASIGLINIATSGQPEEFFT
                     RRVQLETDPQGPTFSPSDRAAILMT"
     gene            744..2030
                     /locus_tag="VI195_01865"
     CDS             744..2030
                     /locus_tag="VI195_01865"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY6823158.1"
                     /translation="MQTPKTASRSAVAKQADPLSDEQLLDLVQRQTFRYFWEGAHPVS
                     GLALDRCHRRTDAPDAPVSIGGSGFGVMALVVACERAWVSREQALSRLALMLDCLEKA
                     TCFHGTYPHFIRGDSGATVPFSRKDDGADLVETSLLFQGLLCARAYFDGDGPHERRLR
                     ERISWLWREAEWDWYTQGGRKVLTWHWSPNNGFSLNNEIRGWNECLITYLLAVSAPRY
                     SLPVEAYHEGWAMSRDFINRRTFEGIELPLGPPWGGPLFFAHFSFCGLDPRGLSDRYA
                     DYWQQNVNHTRINYNYCLRNPHRYKGYGPDCWGLSAGDTPGGYAAHAPDNDLGVINPS
                     AALSSFPYAPREAMSALRHFYDDLGDRIWGRYGFVDGFSEPQGWYADTFLAISQGPIV
                     AMIENYRSGLLWKLFMGIGEIQSGLRKLGFHSPHLP"
     gene            2033..>2941
                     /locus_tag="VI195_01870"
     CDS             2033..>2941
                     /locus_tag="VI195_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY6823159.1"
                     /translation="MPGLEQWIGAEARYAAASMARAISATGLVMERSAFGQRVIPRPG
                     SVLASPVTAHYDPDPDYFFHWFRDSAIVIDALRVALGAGYAEPVAIARFREFVQFSLA
                     LRSIDGQAFLRQADLRAAVRPDFLRYLRSDEELRALSAETVSADTRVNADGTLDFLRW
                     SRPQTDGPALRSIALLRWSRQFPELHADSTLDAALTELLSADLAFTLAFIHRPSGDVW
                     EERDGYHYYTQLLQAEALQRGAEWRASAQPRERTEAWGSAAEETLARLDGFWDHAAGY
                     YRARAATERAEPERELDVSVVLGVLLA"
BASE COUNT          470 a          979 c          974 g          518 t
ORIGIN      
        1 ggcaagcttt cgtggcgcgg tgatgacgat ggataacctc ggcgagagcg ccagccacat
       61 cgatctaaag gagagcgaga gccccgcggt acgcctcgat gagctgcagg tcgcagctcc
      121 cgccggcgct atccagctga gcgagcggca cgtcgatctg caaccgcatg aacgggtcct
      181 gatcagcgcg gaacgtgggg cggcgcgaac gctgctgttc cgggcgatga tcgggctgtg
      241 gccctggggc aagggccgca tcacacgccc ggcgcgggcg gcgatcatgt tcctgcccgg
      301 gcgcgcctat gtgccgccgg ggagcctgcg cgccgcgctc tgttatccat gcaccaccga
      361 cgagttcgac gacgctgcca gcgcgcgggc gctgacagcg gttggcctgg aacggttgca
      421 gccgctgctc gatcgcaccg agcgctggga ccagcgactg aacgatgatg aaaagcagtg
      481 cctcgccatc gcccgcgtca tactgcaacg gccacggtgg gtggtgctcg atggggcgct
      541 ggagctgctc gatggcgcat cgcgccagcg ggtcaaggca ctcttcacca gcgagctcgc
      601 cagtataggt ctcatcaata tcgccaccag cggccagccg gaagagttct tcacgcggcg
      661 cgtgcagctc gagacggacc cgcagggccc gaccttcagc ccgagcgacc gcgccgctat
      721 cctcatgact taggcccgcc atcatgcaga ctcccaagac cgcatcccgc tccgcagtcg
      781 ccaagcaggc cgatccgctc tccgacgagc agttgctcga tctggtgcaa cgtcagacgt
      841 ttcgctactt ctgggaaggc gcacacccgg taagcggcct ggccctcgat cgctgtcacc
      901 ggcgcaccga cgcaccggac gccccggttt ccatcggcgg tagcggtttc ggcgtcatgg
      961 cgctcgtcgt cgcctgcgag cgcgcctggg tgagccggga gcaggcgctg tcccggctcg
     1021 cgctgatgtt ggattgcctc gagaaggcca cctgctttca cgggacctat ccgcatttca
     1081 tacgcggtga cagcggcgcc accgtgccct tcagccgcaa ggacgatggt gccgacctgg
     1141 tggagacttc cctcctcttc cagggactgc tgtgcgcgcg tgcctatttc gacggcgatg
     1201 gcccgcacga gcggcggctg cgcgagcgca tcagctggtt gtggcgcgag gcggaatggg
     1261 actggtacac ccagggcggg cgcaaggtgc tgacctggca ctggagcccg aataacggct
     1321 tctcactgaa caatgagatc cgcggctgga acgaatgcct catcacctat ctgctcgctg
     1381 tgagtgcgcc acgctactcg ctgccggtcg aggcctatca cgagggctgg gcgatgagtc
     1441 gtgacttcat caaccggcgc actttcgagg gcattgagct gccgctcggg cctccctggg
     1501 gcggcccgct gttctttgcg catttctcct tctgcggtct cgatccgcgt ggcttgagcg
     1561 atcggtacgc cgattactgg cagcagaatg tcaaccacac gcgcatcaac tacaactact
     1621 gcctgcgcaa cccgcaccgc tacaagggat acggaccgga ttgctggggg ctgagcgccg
     1681 gtgatacgcc cgggggctac gccgcgcacg caccggataa cgacctcggc gtcatcaatc
     1741 cttcggcggc gttgtcgagt ttcccgtacg cgccgcgcga ggccatgagt gcgctgcgcc
     1801 atttctacga cgatctcggc gatcgtatct ggggacgcta tggcttcgtc gacggcttca
     1861 gcgagccaca gggctggtat gcggacacct tcctcgccat cagtcaggga ccgatcgtcg
     1921 cgatgatcga gaactaccgc agcgggctgc tgtggaagct gttcatgggc atcggcgaga
     1981 tccagtccgg gctgaggaag ctcggtttcc acagcccgca ccttccctga gcatgcccgg
     2041 gctcgagcaa tggatcggcg ccgaggcgcg ctatgccgcg gccagcatgg cgcgcgccat
     2101 ctcggcgacc ggtctcgtca tggagcgcag cgcgtttggg caacgggtca ttccgcgtcc
     2161 gggatcggtg ctcgcctcgc cggtgaccgc tcactatgac ccggaccccg actacttctt
     2221 tcattggttc cgcgactccg cgatcgtcat cgacgcgctg cgcgtcgcgc tgggcgcggg
     2281 ctacgccgaa cccgtcgcta tcgcccggtt ccgcgagttc gtgcagttca gcctggcgtt
     2341 gcgatcgatc gacgggcagg cattcctgcg gcaggctgac ctgcgcgcag cggtacggcc
     2401 ggatttcctc cggtatctgc gctccgatga ggaactgcgc gcgctcagcg ccgagaccgt
     2461 aagtgcggac acgcgcgtca acgccgacgg cactctcgat ttcctccgtt ggtcgcgccc
     2521 gcagaccgac gggccggcgc tgcgcagcat cgcgttgctg cgctggtcgc ggcagttccc
     2581 cgagctacac gcggattcga ctctcgacgc ggcgctcact gagctgctga gcgccgacct
     2641 tgcctttacc ctcgccttta tccatcggcc gagcggcgat gtgtgggagg agagagacgg
     2701 ttatcactac tacactcagc tgctgcaggc agaggcgttg cagcgtggcg ccgagtggcg
     2761 cgcgagcgca caacctcgcg agcgcaccga ggcgtggggt agcgccgcgg aggagacctt
     2821 ggcacggctc gatggcttct gggaccacgc agcaggttac taccgggcgc gagctgccac
     2881 ggaacgcgcg gagccggaac gagagctcga tgtctcggtg gtgctcggtg tgctccttgc
     2941 c
//
LOCUS       DATDMP010000111         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1128668, whole genome shotgun sequence.
ACCESSION   DATDMP010000111 DATDMP010000000
VERSION     DATDMP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128668"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..661
                     /gene="mscL"
                     /locus_tag="VI195_01875"
     CDS             263..661
                     /gene="mscL"
                     /locus_tag="VI195_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015379263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY6823160.1"
                     /translation="MSFASEFKEFAMRGNVIDLAVGVVIGAAFGKIVSSLVGDVIMPA
                     LSPVLGSVNFSELAVRISTDAQGKAVLLKYGVFLQTIFEFIIIAFCIFIVLKGINKLK
                     RPPPPTAAAQPPPTKTESLLQEIRDLLARK"
     gene            complement(673..2031)
                     /gene="pepQ"
                     /locus_tag="VI195_01880"
     CDS             complement(673..2031)
                     /gene="pepQ"
                     /locus_tag="VI195_01880"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HEY6823161.1"
                     /translation="MTRVEVQRSLVSSLDNTFGPHLESVCARTARALERCGYSGLLVY
                     AGAPLPVFEDDRTYPFEANAPFKVWVPLSDAPGSFVWFTPGAVPRLIIERPEDYWYKP
                     TDLPQGYWVRHFDVHTVADRAAARTLLPPDLSRAAYIGDGFDGLGDFGLGAINPRALM
                     HRLDYLRAAKTRYELVCLREANRLGALGHVAAAAAFAAGASEFEIELAFLQGCGLREQ
                     ELPYNPIIGLNAAGAVLHYQVLEKDPPRERHSLLIDAGAEFAGYASDITRTHSRVDQD
                     FAALIARMDEMQQSLCAEVRAGVDWRNVQLRAHELTAGLLRDADLIRCSAAEAVSTGV
                     TRIFLPHGIGHLLGLEVHDVGGFMRAEDGGEIARPEGHPYLRLTRVLEPGFVVTMEPG
                     IYFIPQLLAQARADGRGRHLNWLRIEALARFGGIRIEDDLAVTASGCENLTREAFRFL
                     SQ"
BASE COUNT          423 a          720 c          717 g          384 t
ORIGIN      
        1 gcgtaagaag aagacggcgg cctgacggcg ccggctcggc aacgccgagg caccgagccg
       61 ccgaagcagc gcgccgccga ggcatcgatg atggcggcgt ctcagcgata gttaaccaaa
      121 cagttaacta tggctcgaga cacctgcaag cgcgccgggg ccacgccggc gcgccgcagg
      181 tttgccttcg cgtaaacgct tccgctaggc tctggagcga aagccggcat tcgaaccggt
      241 agtgctcggg gaggagccca tcatgagctt tgcttcagaa ttcaaagaat ttgctatgcg
      301 cggcaatgtc atcgacctgg ccgtcggtgt cgtcatcggc gcagccttcg gcaagatcgt
      361 gtcctcactg gtgggcgatg tgatcatgcc ggcactctcg ccggtgctgg gcagcgtcaa
      421 cttcagcgag ctggctgtca ggatctccac cgatgctcag ggcaaggccg tgttgctcaa
      481 gtacggggtt ttcctgcaga cgatcttcga attcatcatc atcgcgttct gcatcttcat
      541 cgtgctgaag ggcatcaata agctcaagcg cccaccgccg cctactgcag cggcacagcc
      601 accgccgacg aagacggagt cattgttgca ggaaataagg gatttactcg cgagaaagta
      661 atttttgtct cattattggg acaaaaagcg gaaggcctcg cgagtcaggt tctcgcaacc
      721 gctagcggtg acggcgagat cgtcttcgat acggatgcct ccaaagcggg cgagcgcctc
      781 gatccgcaac cagttgaggt gtcgcccgcg gccgtccgcg cgggcctgcg cgagcaattg
      841 aggaatgaag tagatgccgg gctccatcgt gaccacgaag cccggctcca gcacccgcgt
      901 gagtcgcagg tacgggtgtc cttcgggtcg tgcgatctcc ccaccgtcct ccgcgcgcat
      961 gaagccgcct acatcgtgta cctcgagccc gagaagatgc ccgatgccgt gcggcaggaa
     1021 gatgcgcgtc accccggtgc tcaccgcctc cgccgcgctg cagcgaatga ggtcggcatc
     1081 gcgcagcagc ccggcagtga gctcgtgcgc acgaagctgc acgttgcgcc agtcgacccc
     1141 cgcccgtacc tccgcgcaca gggactgctg catctcgtcc atgcgtgcga tgagcgccgc
     1201 gaagtcctga tccacgcgtg agtgcgtgcg cgtgatgtcg ctcgcatagc cggcgaactc
     1261 cgcaccggca tcgatcagca acgagtgccg ctcgcggggt gggtctttct ccagcacctg
     1321 gtagtggagc accgcgccgg ccgcgttcag cccgatgatg gggttgtagg gcagctcctg
     1381 ttcgcgcaat ccgcagccct gcagaaaggc gagctcgatc tcgaattccg atgcgcccgc
     1441 ggcgaaggcc gcagccgccg caacgtgccc gagcgcgccg agccggttcg cctcgcgcag
     1501 gcagaccagc tcgtagcggg tcttcgccgc acgcaggtaa tcgagccggt gcatgagcgc
     1561 gcggggattg atggcgccga ggccaaagtc gccgagcccg tcgaagccat cgccgatgta
     1621 ggcggcgcgg gacaggtcgg gcggcagcag ggtgcgcgct gcagcacgat cggcgaccgt
     1681 atgcacgtcg aaatgacgta cccagtaacc ctgcggcaga tcggtcggct tgtaccagta
     1741 atcctcgggc cgctcgatga tcagccgggg caccgctccc ggcgtgaacc agacgaagct
     1801 gcccggcgca tccgacagtg gcacccacac cttgaagggt gcgttggcct cgaaggggta
     1861 ggtgcgatcg tcctcgaata ccggcaaggg cgcgccggca tagacgagta gcccggagta
     1921 accgcagcgc tcgagtgccc gcgcggtgcg tgcgcagact gactcgagat gcggaccgaa
     1981 ggtgttgtcg aggctgctga ccaggctgcg ttgcacttcg acacgtgtca tttacaaccc
     2041 tcccgtgcaa cctgccgtgt cgcacccgac agacagtgcc cgcaccgcag cggttgacgg
     2101 cgcgagcagt ttccaatcgt accgggactt ctatagaatt tgccgtccgg accggcccaa
     2161 ttagcggtgc ggaacggctc cagccggtcc cagtctcatc aacacaactc ttgcttcatt
     2221 cggggtaccg atcgatgaac acca
//
LOCUS       DATDMP010000112         5024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1684428, whole genome shotgun sequence.
ACCESSION   DATDMP010000112 DATDMP010000000
VERSION     DATDMP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684428"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VI195_01885"
     CDS             <1..1057
                     /locus_tag="VI195_01885"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEY6823162.1"
                     /translation="DKSINVSLADIIYDATKGDFPHPNTMTCKLLGGEQVLLEQEYYS
                     VGGGFIEWKGYKPPKKGQPKYPYATMKELAGHAEKNKLTIAQVILANEVAVSGKSEAD
                     VLAFVDKIQNAMVNIVKSGLAAPAGTLPGPIKLKTKAGDVYKRALQDKFEREQGIGVV
                     SAYALAGSEENARGHLVVTAPTGGSAGVMPAVVYALGEGGRKAPKDKISQGMLTAVGV
                     GYLCKHNATLAAAEGGCQAEIGVASAMAAAMIAEVMGSPPQVVANAAESSLEHHLGMT
                     CDPVAGFVQIPCIERCAYGAVKAWTGYMIASNEIAINRRVDFDSTVTAMALTAKDMNP
                     KYKETSEGGLALSVTLC"
     gene            1097..2347
                     /locus_tag="VI195_01890"
     CDS             1097..2347
                     /locus_tag="VI195_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823163.1"
                     /translation="MKKAGATITVAWLITAIYYFYQYVLRSAPAVMMPQLSEAFGLTA
                     VGVASLAGLFYYGYSPFSLVAGVALDRWGPRTVVPIGAAVIGVGALLFASGNGSLASA
                     GRFIQGAGGVFALLGAAYLATRYFPASRVATLLGATQMFGMAGGFAGQFAVGPAIASG
                     LPWSRFWLYMGFMGVALSILLLVFLPPTQSQEQRGGWVKEAGRSLATVFRNPQSIMCG
                     LIAGLLFIPTSIFDMVWGVRYLQEAHGFDYGEAVMRSSMVPLGWIVGCPLLGLISDHI
                     GRRKPVIAASAAVLLACLAWILFGPHELLPPYVVGFVAGVSSGAAMIPYTVIKEANPA
                     NLSGTATGVVNFLNFTLTALLGPVFSNLLIRASGGAAERTLEHYQAAFQPLMFGVALA
                     IVLTLWLRETGPAATRPAPVAMTS"
     gene            2357..3301
                     /locus_tag="VI195_01895"
     CDS             2357..3301
                     /locus_tag="VI195_01895"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF008852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEY6823164.1"
                     /translation="MVQETGTGKYEALVARCKSLAPVATAVVHPCERSALAGAAEAAA
                     AGLITPLYVGPKARIGEIARENDIALDAAQIIDAPHSHAAAAQAVALVREGRAELLMK
                     GSLHTDELLGAVVARDSGLRTGRRISHVFIMDVPTYHKVLIVTDAAINIAPSLEDKVD
                     ICQNAIDLARSLGLKQPKVAILAAVETVNSKMAATLDAAALCKMADRGQISGALLDGP
                     LAFDNAISKEAARTKGIRSEVAGDPDILLAPDLEAGNILAKLLSFLARADSAGLVVGA
                     RVPIILTSRADSVRARIASCAVAMLAAHARRTPASAKP"
     gene            3388..4581
                     /locus_tag="VI195_01900"
     CDS             3388..4581
                     /locus_tag="VI195_01900"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY6823165.1"
                     /translation="MDEFALVLNSGSSSLKFALFRAAGTDWPMVARGNVEGIGTSPKM
                     SARDGAGGALKTPTLPDGVRDASAALEHVFAWLRANFGGGRIMGVGHRVVHGGPRYGG
                     PAIVTPEVLAELHRLVPYAPLHQPYNLAAIEAVAHHRPDVPQVACFDTSFHRSLPAVA
                     KLVPLPAELRQDGLQRYGFHGLSYEYIASVLPQVAPQIARGRVIVAHLGSGASLCALR
                     AGESIENTFGFTALDGLCMGTRPGAIDPGVVLYLFQGLGLSVKEVETILYKKSGLLGI
                     SGVSNDMRDLLASSEASARLAVDYFVYRVAQEVGALAAVLGGLEALVFTAGIGENSPA
                     IRQRVCAACTWLGIELDEPANAAKRSCISRANSAVSVWMIPTNEELMIARHTGRLLTS
                     ERTRA"
     gene            4611..>5024
                     /locus_tag="VI195_01905"
     CDS             4611..>5024
                     /locus_tag="VI195_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HEY6823166.1"
                     /translation="MAATSPLETLAAADPWRGFHSGIWQKELDVRDFIQQNYTPYEGN
                     ESFLKGATPRTRRIWEKLQALFVEERRKGVLDVSQTPGSITAYAPGYIDRDNEIIVGL
                     QTDAPLKRGILPNGGFRLVVNALKAYDYEPDPKVVE"
BASE COUNT          848 a         1606 c         1680 g          890 t
ORIGIN      
        1 cgacaagagc atcaacgtct cgctggccga catcatttac gatgccacca agggcgactt
       61 cccccatccc aacaccatga cgtgcaagct gctcggcggg gagcaggtgt tgctcgagca
      121 ggagtactac tcggtcggtg gcgggttcat cgagtggaag ggctacaagc caccgaagaa
      181 gggccagccg aagtatccgt atgcgaccat gaaggagttg gccggccacg cagagaaaaa
      241 caagctcacc attgcgcagg tgattctcgc caacgaggtg gcggtgtccg gtaagagcga
      301 ggctgacgtt ctcgccttcg tcgacaagat ccagaatgcc atggtcaaca tcgtcaagtc
      361 ggggcttgcc gctccggccg gcactctgcc cggccccatc aagctcaaga ccaaagcggg
      421 ggatgtctac aagcgcgcac tgcaggacaa gttcgagcgc gaacagggta tcggcgtcgt
      481 gtcagcctac gcgctcgcgg gctcggagga gaacgcgcgc ggtcacctcg tggtcaccgc
      541 gccgaccggc ggttcggcgg gtgtgatgcc ggcggtcgtc tacgcgctcg gtgaaggcgg
      601 acgcaaggca cctaaggaca agatcagcca gggcatgctc accgcggtgg gtgtcggcta
      661 cctctgcaag cacaacgcca cgctcgccgc cgccgaaggc ggatgccagg ccgagatcgg
      721 ggtggcttcc gcgatggccg cggccatgat cgcggaagtc atgggcagtc ccccgcaggt
      781 ggtggcgaac gccgcggaat cctcactgga gcaccatctc ggcatgacgt gcgacccggt
      841 ggcgggattc gtgcagattc cctgcatcga gcgatgtgcc tacggtgcgg tcaaggcttg
      901 gaccgggtac atgatcgcga gcaatgaaat agcgatcaac cggcgcgtcg atttcgacag
      961 cacggtaacc gccatggcgc tcaccgcgaa ggacatgaac ccgaagtaca aggaaacctc
     1021 tgaaggcggc cttgcgcttt cggtaacgct ctgctgacga gcaggatcgg aacggcctgg
     1081 gcactggagg ctcggaatga agaaggctgg ggcaaccatc acggtcgcct ggctcatcac
     1141 ggccatttat tacttctacc agtacgtgct gcgctcggcc ccggccgtga tgatgccgca
     1201 gctctcggaa gcctttggcc tcacggccgt gggtgtcgcc tccctcgccg gtcttttcta
     1261 ctacggctat tcacctttca gcctggtggc cggtgtggcg ctcgaccggt ggggacctcg
     1321 tacggtggtc cccatcggcg ctgcggtgat cggtgttggg gcactgctgt tcgcgagcgg
     1381 caacggcagc ctcgccagtg ccgggcggtt catacaggga gcgggtgggg tgtttgccct
     1441 gctcggcgct gcgtatctcg cgacccggta tttcccggcc tcacgcgtgg cgacgttgct
     1501 cggtgccacg cagatgttcg gtatggccgg gggctttgcc ggccagttcg ccgtggggcc
     1561 tgcgatcgca tccggcctgc catggagccg tttctggctc tacatgggct tcatgggcgt
     1621 cgcgctcagc atcctgctgc tcgtgttcct gccgccaacg cagtcccagg agcaacgcgg
     1681 cggttgggtg aaggaggccg gccggtccct cgctaccgta tttcgtaacc cacagtcgat
     1741 catgtgcggg ctcatcgccg ggctgctgtt cattccgacc tcgatcttcg acatggtctg
     1801 gggggtacgt tacctgcagg aggcgcacgg gttcgactat ggggaggcgg tgatgcgctc
     1861 ctcgatggtg ccgcttggct ggatcgtcgg ctgtccgctg ctcggtctca tctccgacca
     1921 catcggccgg cgcaaacccg tgatcgccgc gagcgcggcg gtgctgctcg catgcctcgc
     1981 ctggattctg ttcgggccgc acgagctgct gccgccgtac gtcgtcggtt tcgtcgccgg
     2041 cgtgtcatcg ggagccgcca tgatccctta tacggtgatc aaggaagcga acccggcgaa
     2101 cctgagtggg acggccacgg gtgtggtgaa cttcctgaac ttcacgctga ctgcgctgct
     2161 gggcccggta ttcagcaacc tgctcattcg cgcctcgggg ggtgccgcgg agcgcacgct
     2221 ggagcattat caggccgcgt tccagccgtt gatgttcggg gttgcactcg ccatcgtgct
     2281 caccctgtgg ctgcgcgaga ccggccccgc agcgacacga ccggcgccgg tggcgatgac
     2341 atcatagggg acgtccatgg tccaggaaac cggcaccggc aaatacgagg cgctcgttgc
     2401 gcgctgcaag agcctggccc ccgtggccac cgcggtcgtg cacccgtgcg agcgcagcgc
     2461 actcgcgggc gccgcggagg cggcggccgc ggggctcatc actcctcttt atgtcggacc
     2521 gaaggcgcgc atcggggaga tcgcccgtga gaacgatatc gcgctcgacg cagcgcagat
     2581 catcgacgcc ccccacagtc acgcagccgc cgcgcaggcg gtggcactgg tgcgcgaggg
     2641 ccgggcggag ctgctgatga aggggagcct gcataccgat gagctgctcg gtgcagtcgt
     2701 ggcgcgtgac tccgggctgc gcacgggccg gcgcatcagc cacgtgttca tcatggatgt
     2761 gccgacttac cacaaggtac tgatcgtcac cgatgcggcg atcaacatcg ccccatcgct
     2821 cgaggacaag gtggatatct gccagaacgc catcgacctg gcccgctcgc tggggctcaa
     2881 gcagcccaag gtcgccatac tggcggcggt ggagacggtg aattccaaga tggccgccac
     2941 gctcgacgcc gcggcgctct gcaagatggc cgaccgcggg cagatcagcg gggctctgct
     3001 cgatgggccg ctcgcgttcg ataacgccat cagcaaggag gcggcgcgca ccaagggcat
     3061 ccgctcagag gtggccggcg atccggacat tctccttgcg ccggacctcg aggccggcaa
     3121 tatcctggcg aagcttttga gttttcttgc ccgggcggac agcgccggtc tggtcgtcgg
     3181 ggcacgcgta cccatcattc tcaccagtcg cgccgacagc gtacgcgccc gcatcgcgag
     3241 ctgcgcggtg gcgatgctcg cggcacatgc tcgccgcacg ccggcgagcg cgaagccatg
     3301 agggggaagt atcaacgtgt tgataccgcg ccatatcgac ctatttgtgg ccgggtgcca
     3361 tcaggcacgg atcacaggaa gtgaagcgtg gatgagttcg cgctcgtact gaattcgggc
     3421 tcctcgagcc tcaagttcgc gctgtttcgc gccgcgggca cagactggcc gatggtggca
     3481 cgcggcaacg tcgaaggcat cggcacctcg ccgaagatgt cggcgcgcga tggggcgggg
     3541 ggcgcgctga agaccccgac actgccggac ggtgtacgcg acgcgagcgc cgcactcgag
     3601 catgtgttcg cctggttgcg agcgaacttc ggcggcggcc gcatcatggg cgtgggccac
     3661 cgcgtggtgc acggcggtcc ccgctacggc ggtcccgcga tcgtcacacc ggaagtgctg
     3721 gcggagcttc accggctcgt gccctatgcc ccgctgcacc agccttacaa cctggcggcg
     3781 atcgaggcgg tggcgcacca caggcccgat gtgccgcagg tcgcctgctt cgataccagc
     3841 tttcatcgca gtcttccggc cgtcgcaaaa ctggtacccc tgcctgctga gctgcgccag
     3901 gacggcctgc agcgttatgg cttccacggt ctctcgtacg aatacatcgc ctccgtgctc
     3961 ccccaggtcg caccacagat cgcgcgcggg cgcgtgatcg ttgcgcacct cggcagcggc
     4021 gcgagcctgt gcgcgttgcg agccggcgag agcatcgaga acaccttcgg gttcactgcg
     4081 ctcgatggcc tgtgcatggg cacgcgaccg ggcgccatcg atcccggcgt ggttttgtat
     4141 ctctttcagg gactcggcct ctcggtcaag gaggtcgaga ccatcctgta caagaagtcg
     4201 ggcctgctcg gcatctcggg agtcagtaac gacatgcgcg acctgctcgc gagcagtgag
     4261 gcctctgccc gcctcgccgt ggactatttc gtctatcgag tggcgcagga ggtcggcgcg
     4321 ctcgcggcgg tgctcggtgg gctcgaggcg ctggtgttca ccgccgggat cggcgagaac
     4381 tcacccgcca tccggcagcg cgtctgcgcc gcctgcacct ggctcggcat cgagctcgat
     4441 gagccggcca acgctgcgaa gcggtcctgc atctcccgtg cgaacagcgc agtatcggtg
     4501 tggatgatcc cgacgaacga ggaactgatg atcgcccggc ataccgggcg attactgaca
     4561 tcggaacgaa cccgcgcgta gcgggcgatc gctccttgag gagtctgttt atggccgcaa
     4621 cgtcaccgtt ggaaaccctg gccgccgcgg atccgtggcg cggcttccat tccggaatct
     4681 ggcagaaaga gctcgatgta cgcgatttca ttcagcagaa ctacaccccg tacgagggta
     4741 atgagtcgtt tctcaagggt gcgacacccc gtacgcgccg catctgggag aagctccagg
     4801 cgctattcgt cgaggagcgg cgcaaaggag tgctcgatgt gtcgcagaca cccgggtcga
     4861 tcaccgccta cgcacccggt tatatcgatc gtgacaacga gatcatcgtc gggctgcaga
     4921 cggatgcacc gctcaagcgc ggcatcctgc cgaatggcgg cttccgtctg gtcgtaaacg
     4981 cgctcaaggc gtacgactac gagcccgacc ccaaggttgt cgag
//
LOCUS       DATDMP010000113         5656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_906957, whole genome shotgun sequence.
ACCESSION   DATDMP010000113 DATDMP010000000
VERSION     DATDMP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906957"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VI195_01910"
     CDS             complement(<1..936)
                     /locus_tag="VI195_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6823167.1"
                     /translation="MTASPGNLRVLIIVENLPCPFDRRVWQEALALRACGHEVSIICP
                     KGRGYTKGFEVLEGVYIYRYSLPLEADSAIGYALEYAASLYMEFALSLWVALTRGFDV
                     IHGCNPPDTIFLIALFYKSFGKRFIFDHHDINPELYEAKFGRRDFWYRLVCRLERWTF
                     ATADVSIATNESYRRIAIERGGMEPARVFVVRSGADLTRVHTVPARSELRRGKRYLVG
                     YVGVIGKQEGLDLLLESIAYMRHKLQRADTHFVIVGDGTELVALRQLSRELAIEDCVE
                     FTGRLPDGPLWEILSTADVCVNPDRANSMNDMSTMN"
     gene            complement(917..2239)
                     /locus_tag="VI195_01915"
     CDS             complement(917..2239)
                     /locus_tag="VI195_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY6823168.1"
                     /translation="MKIAVFGLGYVGAVSAACLARNGHYVTGVDPNTTKVDFIRRGKS
                     PIVEPGLEDLIAAAVGDGRLVAYPDHAPAVAQSEILMVCVGTPGNANGSLDLSYVRRI
                     VEQIGEVLATVADYKVVVIRSTLLPGSMQSVVVPILEQHSGRQCGRDFGICLNPEFMR
                     EGTAIHDYDHPPKTVLAASDERALGLMRALYADLDAPLIVTNVPTAEMIKYIDNCWHA
                     LKVTFANEVGRLCKAMGVDGQLAMRLFCADTKLNLSSAYLRPGFAFGGSCLPKDVRAL
                     TYQGRLLDVDTPVLGSILASNQVHIAQAFSMIRATGRRRIGLLGLSFKESTDDLRESP
                     LVTLAELLIGKGYELCVYDRNVRLASLLGANREYILNQIPHIGRLLVDRPEQLFEQSE
                     VVVVAAREGDFGSLLRDFARQKTIIDLVGVRDISEDGRSTHNYDGIAW"
     gene            complement(2442..4178)
                     /locus_tag="VI195_01920"
     CDS             complement(2442..4178)
                     /locus_tag="VI195_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823169.1"
                     /translation="MNLLKARHVALLVGLLTGSMVAEGKDIYVAASGGSDENSGSTPS
                     APLASIAAASSRAAAGDNVYLMSGQYHEAIVPVNSGTAAKPITYKSYGRGAAVISNVK
                     IGILVSSQSYITFDGINVNGGNLPPKATVMSFAGIQNSNHITIRHGEFRNANGWAGID
                     ISGRYLPDGRYWGVNPNAALEGVSAYITIEDNVLDNVGVYANPMTGDVIQVAIGVNQH
                     ILIQRNTITHGGHDLVELDSDYGVLQDNVLNNYYGDQVGGDTGYRSLEVQGSFNVIQR
                     NFMAHSRVGDGIIAAIASIRGSQNLARQNILFDGISSGFGTWCASGRSPAATDGRIYN
                     NTIYQTGGEGWYMWAYDGCTGIGGYVFANNLLVNSRTSLASTGVPRDGTLKDAELVLI
                     PLAGDSSATGQSIVKGNLFAPAPGGPAYVITSRDGRIPLRTAASKYPQSFIANTEARP
                     TLISAKPSVAADFQLSPGSPGRGAGVFLTNVVGSGTSNHLAVRDSLYFSDGNGVVPGD
                     MIQLQGTAGPVQILSIERNSNTLTLSSPVTFKDGQGVALPYTGAVPDVGAGLVNPAAV
                     RPMPPANVSIGH"
     regulatory      complement(4370..4458)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4852..>5656
                     /locus_tag="VI195_01925"
     CDS             4852..>5656
                     /locus_tag="VI195_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823170.1"
                     /translation="MPKSRALAAPADFINRRSLIRLPASSGGTTGTPLRLWRSLECVV
                     AEQSFLDQLLAAYDCSMRSRVAVLRAERVKPAAEAGPPYGRLSHGGMRLTLSSLHLNA
                     ESLPWYVDALETFAPQVLWVYPSAAINLLRLMERAALRLSLPVILASSELLSARMHAE
                     LERFFSARVINHYGQAERVCFAWSTRPEEFFFHPLYGRVELQMVAASDPPAARIIGTG
                     FWNTAMPLIRYDTGDLLYLPARLGADELAEIARGERPFAGLAGRSGEYLL"
BASE COUNT         1089 a         1747 c         1692 g         1128 t
ORIGIN      
        1 gttcatcgtg gacatgtcgt tcatcgaatt tgcacggtcg ggattcacgc agacgtccgc
       61 cgtgctcaga atctcccaca atggaccatc aggaaggcgg ccggtaaatt ctacgcagtc
      121 ttcgatcgcg agctcccgtg acagctgacg aagcgccacg agctcggtgc cgtcacccac
      181 tatcacaaag tgcgtatcgg cacgctgcaa cttatggcgc atgtaagcga tcgactcgag
      241 caacagatcg agcccttcct gcttgccgat caccccaaca tagccaacca ggtagcgctt
      301 gccgcgccgt agttcgctgc gtgcgggcac cgtgtgtacg cgcgtgagat cggcgccgct
      361 gcgcaccacg aagactcttg cgggctccat gccgccacgc tcgatcgcga ttctgcgata
      421 tgactcgttg gtggcgatgc tcacatcagc agttgcgaac gtccagcgtt ccaggcggca
      481 taccaaacga taccagaagt cccgccggcc aaacttggcc tcgtagagtt ccgggttgat
      541 gtcgtgatgg tcgaaaatga agcgcttgcc gaaagacttg tagaacagtg caatgagaaa
      601 gatcgtatcg ggtgggttgc agccatgtat cacatcgaag ccgcgcgtca atgccaccca
      661 taacgacagc gcgaattcca tatagagcga tgcggcatat tcaagcgcgt aaccgatggc
      721 cgagtcagcc tccagcggca gggaataacg gtagatgtag acaccctcga gtacctcgaa
      781 acccttggtg taaccacgcc ccttcggaca gatgatgctg acctcatggc cgcaggcgcg
      841 cagtgccagc gcttcctgcc acacccgccg gtcgaacggg cagggcaggt tctcaacgat
      901 gatgagaact cgaaggttac caggcgatgc cgtcatagtt gtgtgtcgat ctgccgtctt
      961 cacttatgtc ccgcacgccg actagatcta tgattgtctt ctgccgcgcg aaatcccgga
     1021 gcaatgagcc gaagtcccct tcgcgcgctg ccaccaccac cacctcggac tgctcgaaca
     1081 gctgctccgg ccgatcgacc aacagtcgtc cgatgtgtgg aatctgattc aggatgtact
     1141 cgcgattcgc gcctaacagg cttgccaacc tgacgttgcg gtcatacacg cagagttcgt
     1201 agcccttgcc tatgagcaat tcggccagcg tcaccagcgg gctttcccgc aggtcgtccg
     1261 tgctctcttt gaagctgagg cccaacaggc ctatgcggcg ccggccggtt gcgcgaatca
     1321 tggaaaaggc ctgggcgatg tgcacctggt tgctcgccag gatggagccg agcaccggcg
     1381 tgtctacgtc gagcaatctg ccctggtagg tgagtgcccg cacatctttc ggcaggcagg
     1441 agccaccgaa tgcaaagccc ggtcgcagat aggcacttga caggttgagc ttggtgtccg
     1501 cgcagaagag ccgcatggcc agctgcccgt ccacgcccat ggccttgcac agtcggccca
     1561 cctcgttcgc gaatgtgacc ttgagcgcgt gccagcagtt gtcgatgtac ttgatcatct
     1621 ccgccgtcgg cacgttggtc acgatgagcg gcgcgtccag atcggcgtac agcgcgcgca
     1681 tcagcccaag cgcgcgctcg tccgaggcag ctaacaccgt cttcggtgga tggtcgtagt
     1741 catgaatagc cgtgccttca cgcatgaatt ccggatttag acagattccg aaatcccggc
     1801 cgcactgccg acccgaatgt tgctcgagaa tggggaccac cacgctctgc atcgagcccg
     1861 gtaacagagt gctgcggatt accacgacct tgtaatcggc aacagttgcg aggacttcgc
     1921 cgatctgttc tacgatccgg cgcacgtagc tcaagtccag gctgccgttg gcgttgccgg
     1981 gcgtgccgac gcacaccatc aggatttcag actgcgcaac cgccggcgcg tggtccggat
     2041 atgcaaccag cctgccgtcg ccgaccgctg cagctatcag gtcttcaaga cctggctcca
     2101 cgatcgggct cttgccgcgg cggatgaaat cgaccttggt cgtatttgga tcgacgcctg
     2161 ttacataatg cccgttgcgg gcgaggcagg cggcggagac tgcccccacg taaccaaggc
     2221 cgaagactgc tattttcatt cttgttattt gcccttgcgg tgctagggcg caacgaatat
     2281 ctcgatgcgc gctgatcgca acacccgccc gcagttgtaa cccgcgggcg acttcttagt
     2341 ttataggcta tcccccggcc agagcgtgtt acttagtata tcagcccggg aggaaggtca
     2401 actggatctg tgtttcgtat cttatgggtt atgtttagtc tttaatgacc aatacttacg
     2461 ttcgccggcg gcataggccg cactgcggcc gggttcacta gaccggctcc aacatccggc
     2521 accgcgccgg tgtagggcag tgcgactccc tgtccatcct tgaatgtgac cggcgaagac
     2581 aaggtcaatg tattcgagtt ccgctcgatg ctcaggatct gaaccgggcc agcggtgccc
     2641 tgcaactgaa tcatgtctcc gggaacgact ccgttaccat cgctgaagta aaggctgtcg
     2701 cgcacggcaa ggtggttgga ggttcccgag cccaccacgt tggtgagaaa cacgccagcg
     2761 ccacgacccg ggctgcccgg tgacagctga aagtcggccg cgacggaggg cttcgcactg
     2821 atgagggtcg gccgggcctc ggtgttggcg atgaatgact gcggatattt gctcgccgcc
     2881 gtacgtagcg gaatgcggcc gtccctgctg gtgatcacgt acgctggccc accaggagcc
     2941 ggtgcgaaca ggttgccctt cacgatactc tgcccggtcg cagacgagtc tccggcaagc
     3001 ggtatcagga ccagctcggc gtccttcagt gtgccatcgc gcggcacgcc ggttgacgcc
     3061 aggctggtac gtgagttgac cagcagattg ttggcgaata catagccgcc gatgccggta
     3121 cagccatcgt acgcccacat ataccagccc tcgccgccgg tttgatagat cgtgttgttg
     3181 tagattctgc cgtcagtggc ggccggcgat cgccccgaag cacaccatgt accaaagccg
     3241 gaggatatgc cgtcgaaaag aatgttttgc ctggcgagat tctgactgcc gcgtattgag
     3301 gcgatggcgg caatgatgcc gtcacccacg cgcgagtggg ccatgaagtt acgctggatg
     3361 acgttgaacg agccctgcac ctccaggctg cgatagccgg tgtcgccgcc cacctggtcg
     3421 ccataatagt tgttgagtac gttgtcctgc agcaccccgt agtcggaatc cagctccacc
     3481 aggtcgtggc cgccgtgagt gatggtgtta cgttggatca ggatgtgctg gtttacgccg
     3541 atggcgacct ggatcacgtc gccggtcatg gggttggcat agacgcctac gttatccagc
     3601 acattgtcct cgattgttat gtaggcggat acgccctcga gcgcggcatt ggggttgact
     3661 ccccaatatc tgccgtcggg cagatagcgg ccggagatat cgatccccgc ccagccgttg
     3721 gcattcctga actcgccgtg cctgatggta atgtggttcg agttctggat gcccgcaaaa
     3781 ctcatgacgg tggctttggg aggtagattt cccccattga cgttgatgcc atcgaacgtg
     3841 atgtatgact gcgagctgac cagaattccg attttgacgt tggaaatgac ggccgcgcct
     3901 cggccgtaac tcttgtaggt gatcggtttg gctgcagttc cggagttcac cggaacgatc
     3961 gcctcatggt actgacccga catgagatag acgttgtcgc ctgccgctgc ccggctcgac
     4021 gcggcggcga tgctcgccag cggtgcgctc ggagtgctgc cggaattctc gtcgctgccc
     4081 ccgctggcag cgacatagat gtctttgccc tcggccacca tcgacccggt cagcaaacca
     4141 acgagcaacg ccacgtggcg ggctttcagc aggttcatcg tatgtcagtc tccaaactca
     4201 gccagtaacg agcgcgcatg ggccgctgcc gcgagtgcac cagcgagcgg caaacccgcc
     4261 gatgccgtgt ttacgccagt acgtcgcggt gaagaagcgg agacggcaag tccgaagcgg
     4321 agggcggctc ggcagaacag cggtgcatcg cgcatcaacc ccatttcccg ccggcaaccc
     4381 cgctatccca tgcatgctca tgcgtcagga ccggaggctt tgcgtccccg cctcgcgacg
     4441 ggtttgccaa aacacgcaaa ggactatagc gttcggttta aacaataagc aactgtatca
     4501 aggcggcgac aacttcgagt acatctgcct tgggcaccgt gcaacgaccc cacacaacat
     4561 attccggagc agaacatctt ggtgacctcg ccagcagctc actggcaccg cgagcatatg
     4621 aatagacggc ggctcaccag actctcggcg cacgcaaaat ccgtcggcct agtggatctc
     4681 gcggtgagat acaacccttg gttacgtgga cgtgtgagca gcgcattgca cgccgcggac
     4741 gccggcgcac gcagcgcaca ggtcgagtgg ttgagccggc gcatgctccg ctgcgcccgg
     4801 caaacacgct acggctcggg ccgatcgcaa cggctggacg agtggccggt attgccgaag
     4861 agccgagcgc tggctgcacc ggcggacttc atcaatcgac gcagcttgat acgcctcccg
     4921 gcaagcagcg gcggcacgac cggcacgccg ctgcgactct ggcgctcgct ggaatgcgtc
     4981 gttgcggagc agtcgtttct cgaccagctg ctggctgcct acgactgcag catgcgctcc
     5041 cgggtagcgg tgttgcgcgc ggagcgggta aagcccgctg ccgaagccgg cccgccctat
     5101 gggcggttga gccatggggg catgcgcctg acactttcct cattgcatct gaatgccgaa
     5161 tcgctcccct ggtacgtcga tgcgctcgag acctttgcac cgcaggtcct gtgggtgtat
     5221 ccgagcgcag caatcaacct gctgagattg atggagcgcg ccgccctgcg actttcgctg
     5281 ccggtgattc tcgcctcctc ggagctcctc agcgcccgca tgcacgccga gctcgagcgt
     5341 tttttcagcg cgcgggtaat caaccactac ggccaggcgg agcgcgtatg tttcgcgtgg
     5401 tctacccggc cagaggagtt tttcttccac ccgctgtacg gcagggtcga gctgcagatg
     5461 gtcgccgcct cggatccgcc ggcagccagg ataatcggca ccgggttctg gaatacggcg
     5521 atgccgctca ttcgttacga taccggcgat ctgctgtacc tccctgcccg gctcggtgcc
     5581 gacgagctcg ccgagattgc ccgcggcgag cgtcccttcg ccgggctcgc cggcagaagt
     5641 ggtgaatacc tgctca
//
LOCUS       DATDMP010000114         3058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1906402, whole genome shotgun sequence.
ACCESSION   DATDMP010000114 DATDMP010000000
VERSION     DATDMP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906402"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VI195_01930"
     CDS             <1..582
                     /locus_tag="VI195_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6823171.1"
                     /translation="TDPARAGLGGCVADIGTHAANLLEYVTDRPIEALSADLSSFVAG
                     RRLDDDAHMMLRLQGGAKGTLICSQVACGEENRLSLRIYGSQAGLEWHQQEPNTLLFK
                     PAGRPWECLRTGHSYLGAAARAAARVPPGHPEGYLEAFANLYRGFMQDVWRVAAGQAP
                     VRDYPGVQQGLRGLRFVAQAVASSRSNSSWQSV"
     gene            586..1839
                     /locus_tag="VI195_01935"
     CDS             586..1839
                     /locus_tag="VI195_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEY6823172.1"
                     /translation="MSKSIRVRLCVMMFLEFFIWGGWFVTLGSYLAANLHASGAQSAL
                     AYSTQSWGAIIAPFIVGLIADRYVNAERLLGALHIAGGLLMYALYGARDFAAFYPWVL
                     TYMILYMPTLALVNAIAFRQMAEPARHFSGLRVWGTIGWIVAGLVISYLFAWDSRSGI
                     GAGLLRNTFLMCALASFALGLYSFTLPRTPPQPRTGEPLRLKHLLGLDALQLLRQRNF
                     GIFFVASILICIPLAFYYQNANEFLTEIQVANATGKQTIGQMSEVLFMLLIPVFLRTF
                     GMKTTLLVGMLAWAGRYLLFAFGNAQELAFMLIVGIALHGVCYDFFFVSGQIYTDSKA
                     GPTYKAAAQGLITLATYGLGMLIGFSAAGLIDDLYFAGGRHDWRAIWLYPAAFAGAVF
                     VLFALTFRNEKVAYDVSAAPSSGRA"
     gene            1928..>3058
                     /locus_tag="VI195_01940"
     CDS             1928..>3058
                     /locus_tag="VI195_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY6823173.1"
                     /translation="MKLSILTAALQELTPRERRDADPDLAIEEWLEFAVQIGAPNIEL
                     SAALHPSESDVPAAALLDPVANTLDLRAPFSAARAARVRRAMQATGVGLSDLAYFDNM
                     LAADESARGKKYALMLRVFDAAVLLGTNAVTGFVGRNPQLGMDANLTMFEEVFVPLLA
                     QAKARGLTFRVEQCPMPGWNVSDSWHNNIAYAPGPWIALHRICERHGVGDQFRIHYDP
                     SHAILMGQDTRSLFQYLKDTGYDFLIGGFHVKGQVIDARGIAAWGYGGQTMQRGDRDG
                     DRPSTDPAARQNAWKKQTVFAEHELPGTARHDPLGYLQNRTVDWLDHQLAARELLRIE
                     PQKTFLVLEHEYPKARIQDKARLAPILAGSLAFTRAIDEAAAA"
BASE COUNT          485 a         1022 c          988 g          563 t
ORIGIN      
        1 accgatccgg cacgggccgg actcggtggt tgtgtggcgg acatcggcac gcacgcagcc
       61 aatctgctcg agtacgtaac cgatcggccg atcgaggcgc tctcggccga cctcagctcc
      121 ttcgtggccg gcaggcggct cgatgatgac gcgcacatga tgttgcgctt gcagggcgga
      181 gcgaaaggaa cgctgatctg ctcgcaggtc gcctgtggag aggagaaccg cctgtcgctg
      241 cgcatctatg gatcgcaggc gggcctcgaa tggcaccagc aagagcccaa cacgctgctg
      301 ttcaagcccg cgggccgtcc ctgggagtgt ctgcgaactg gccacagcta cctgggagcg
      361 gcggcacgcg ccgcggcacg agtgccgccc ggtcatccgg aaggctatct cgaggctttt
      421 gccaacctct accgcggttt catgcaggac gtgtggcgcg tggcagcggg gcaagcgccg
      481 gtgcgcgatt acccgggcgt gcagcagggt ctgcgcggcc tgcgtttcgt ggcgcaggcg
      541 gtggccagct cccgcagcaa ctcgtcgtgg cagagcgtgt aagagatgag caagtccatc
      601 cgggtgcggc tgtgcgtgat gatgtttctc gagttcttca tctggggagg ctggttcgtc
      661 acactcggta gctatctcgc tgcaaacctg cacgccagcg gtgcccagag cgcgctcgcc
      721 tactcgacgc aatcctgggg cgcgatcatc gcacccttca tcgtcgggct cattgccgac
      781 cgctacgtca acgccgagcg gctcctcggc gcgctgcaca tcgccggcgg cctgctcatg
      841 tacgcgctct acggggcgcg cgatttcgcc gccttctacc cttgggtgct cacctacatg
      901 atcctgtaca tgccgacgct cgctctcgtg aacgccatcg ccttccggca gatggccgag
      961 ccggcgcggc acttctccgg gttgcgcgtg tggggcacga tcggctggat cgtcgccgga
     1021 ctcgtgatca gttatctgtt cgcctgggat tcgcgcagcg gcatcggtgc gggactgctg
     1081 aggaacacct ttctaatgtg tgcgctggca tccttcgcgc tcggcctcta cagcttcacg
     1141 ctgccacgca cgccgccgca gccacgtacc ggggagccgc tgcgtctgaa acacctgttg
     1201 ggactcgatg ctctgcagct gctgcggcaa cgcaacttcg gcatcttctt cgtcgcctcg
     1261 atcctcatct gcatcccgct cgccttctac tatcagaacg ccaacgagtt cctcaccgag
     1321 attcaggtcg ccaatgccac cggcaagcag accatcggcc agatgtccga ggtgctgttc
     1381 atgctgctga tcccggtgtt cctgcgcacc ttcggcatga agacgaccct gctcgtcggg
     1441 atgctcgcct gggccggccg ctacctgctg tttgcgttcg gcaacgcaca ggagctcgcc
     1501 ttcatgctca tcgtcggtat cgcactgcac ggggtgtgct acgacttctt cttcgtctcc
     1561 ggtcagatct acaccgactc caaggcaggc cccacgtaca aggcggcggc gcaggggctg
     1621 atcacgctcg cgacctatgg gcttggcatg ctgatcggtt tctcggccgc gggcttgatc
     1681 gacgatctct acttcgccgg cggccgtcac gactggcgcg cgatctggct ctacccggcg
     1741 gccttcgcgg gtgcggtgtt cgtgctcttc gcactgacat tccgcaacga gaaggtcgcg
     1801 tatgatgtct cagcggcccc atcgagcggc cgcgcctgag caccggggtc aagagcacgg
     1861 catgacgaag ggcgaggaga gttcacgggc cgcacggccg gccgtggccg ctttccggcc
     1921 gcggcccatg aagctcagta ttctcaccgc tgcgttgcag gagctcactc cgcgcgagcg
     1981 gcgcgacgcc gacccggacc ttgccatcga ggagtggctg gaattcgccg tgcagatcgg
     2041 cgcgccgaac atcgagctct ccgcggcctt gcacccgagt gagagcgatg tgccggcggc
     2101 ggcgctgctc gatccggtcg ccaataccct cgacctgcgc gcgcccttca gcgcggcacg
     2161 cgcggcgcga gtccgccgcg ccatgcaggc gaccggggta gggctctcgg atctggccta
     2221 cttcgacaac atgctggcag cggatgagtc cgcgcgcggc aagaaatacg ccctgatgct
     2281 gcgcgtgttc gatgccgcgg tgctgctcgg caccaatgcc gtcaccgggt tcgtcgggcg
     2341 caatccgcag ctcggcatgg acgcgaatct caccatgttc gaggaggtat tcgtaccgct
     2401 gctcgcgcag gcgaaggcgc gcgggctcac ctttcgggtc gagcagtgtc ccatgcccgg
     2461 ctggaacgtg agcgacagct ggcacaacaa catcgcttac gcgccggggc cgtggatcgc
     2521 gttgcaccgc atctgcgagc ggcacggggt cggcgatcag ttccgcattc attacgatcc
     2581 ctcgcacgcg attctgatgg gccaggatac gcgctcgctg tttcaatatc tgaaagacac
     2641 gggttacgac tttctgatcg gcggctttca cgtcaagggg caggtcatcg atgcccgcgg
     2701 cattgcggcc tggggctatg gcggtcagac catgcagcgc ggtgatcgcg atggcgaccg
     2761 gccgagcacc gatccggcgg cccggcagaa cgcctggaag aagcagacgg tgttcgccga
     2821 gcacgagctg cccggcaccg cacgccacga tccgctcggc tacctgcaga accgcaccgt
     2881 cgattggctc gaccatcagc tcgcggcacg cgagctgctg cggatcgagc cgcagaagac
     2941 tttcctcgtt ctcgagcacg aatacccgaa ggcccgtatc caggacaagg cgcgcctcgc
     3001 gcccatactc gcgggctcac tcgccttcac gcgtgcgatc gatgaggcgg cggcggcg
//
LOCUS       DATDMP010000115         2843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_685136, whole genome shotgun sequence.
ACCESSION   DATDMP010000115 DATDMP010000000
VERSION     DATDMP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685136"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..2247
                     /locus_tag="VI195_01945"
     CDS             154..2247
                     /locus_tag="VI195_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY6823174.1"
                     /translation="MAVIGAAALAAAPGASAAEVGVIPLPASITSRPGYFVLRGGTEV
                     YAGADARSQRTAAYFTELLRTSHGIGLKVHTLPQADVPAAGVAAHAILFRLDPQAPGT
                     SPESYQVDVSREGVRVSAREPQGLFYGAVTLWQLCSAPAAHAEIELAATLISDTPRFR
                     WRGLMLDSARHFQSPQFVMRYIDWMALHKLNVLGWHLTDDQGWRLEIRKYPRLTAVGA
                     WRVEAGEAARRDIDPATGRPRLYGGFYTQKDVRRIVAHARERYVTIVPEIDMPGHASA
                     AIVAYPSLGVTDHPPRAVPADWGVFTNLYNVEESTFSFLENVLAEVMELFPSEFIHVG
                     GDEAVKDQWKASPRVQARMRELGVPNEEALQGYFTHRLGEYLEAHGRRLIGWDEILDG
                     GVPPGAAVMSWRGVEGAIAAAASGHDTVLSPAPTLYLDFRQGAGPKEPPGRGEVVSLE
                     RVYRFDPLPAPLAAAAGHVLGVQANLWTEHVRTEEWAAYMTWPRAAALAEIGWSPAER
                     RDYADFQRRLPAAFDRYRTLGIAFSADVFAPPRTLGVWDRHMSQDLASCTEKSILNLE
                     DDAPLEGARAVFLIDIENPCWMMPAADLSSARKLTAAVGQVPFNFQIGHDVDAITLRP
                     PRSAAGELEVRLDHCDGAPLAVLPLAPAAGHDAVTVLPAVALPPLQGSHALCLTFTQR
                     TLDPLWALDWVQLSP"
     gene            2244..>2843
                     /locus_tag="VI195_01950"
     CDS             2244..>2843
                     /locus_tag="VI195_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEY6823175.1"
                     /translation="MRPSDTLTLLAPLAGWSAPLEEAPDEVFAARLLGDGVAIDPTEG
                     TLHAPCDAEVVLLPASRHAVTLRTVAGCRILLHVGIDTVALGGEGFQAHVSQGARVRA
                     GDLLLSFDLDFLAQRAPSVLTPVIITSDSGCAIRTRTVDRAVALGEPLMEVTVLQALP
                     DDSSRSRATAAVVATRTLKVSFEHGIHARPAALLAGSLRS"
BASE COUNT          402 a         1009 c          966 g          466 t
ORIGIN      
        1 gccggtaact gcccacattc ccgagtcccg gcgcggggtg ctggggcggc tcgctgccgc
       61 ggatgcgcag cggctgcagg cgcagcttgc ccagcggctg cagccgcgca agtgatgcgt
      121 cgcgccgcgc tcgtacgctt gcgcctcgtg gcgatggctg taatcggcgc cgccgcgctc
      181 gcagccgccc cgggtgcatc ggcagccgag gtcggtgtca ttccgcttcc cgccagcatc
      241 acctcccgcc ccggatactt cgtgctgcgc ggcggcaccg aggtctatgc cggcgccgat
      301 gcgcgatcgc agcgcaccgc tgcttacttc accgagctgc tgcgcacgag tcacggcatc
      361 ggcctcaagg tgcacacgtt gccgcaggcc gacgtgccgg ccgcgggcgt ggccgctcac
      421 gccatcctgt tccgcctcga tccgcaggct ccgggcacga gcccggaaag ctaccaggtc
      481 gatgtctcgc gcgaaggcgt gcgggtctcg gcgcgcgagc cacagggcct gttctacggc
      541 gccgtcacgc tctggcaact ctgcagtgca ccggcggcgc acgccgagat cgagctcgcc
      601 gccacgctca tcagcgacac accgcgtttc cgctggcgcg gactgatgct cgattcggcg
      661 cgccacttcc agtcgccgca gttcgtgatg cgctacatcg actggatggc gctgcacaag
      721 ctgaatgttc tcggctggca cctgaccgac gatcagggct ggcggctcga gatccgcaag
      781 tacccgcgcc tgaccgccgt gggcgcctgg cgtgtcgaag cgggcgaggc ggcgcggcgc
      841 gatatcgatc ctgcgactgg ccgcccgcgc ctgtacggcg gcttctacac ccaaaaggat
      901 gtgcggcgca tcgtcgcaca tgcccgcgag cgctacgtca ccatcgttcc ggagatcgac
      961 atgcccgggc acgcaagcgc ggcaatcgtc gcctacccga gccttggcgt cacggaccat
     1021 ccgccgcgcg cagtgcccgc cgactggggc gtgttcacga atctctacaa cgtggaggag
     1081 tccacctttt cctttctcga gaacgtgctc gcggaggtca tggagctctt cccgagcgag
     1141 ttcatccatg tcggcggcga tgaggccgtg aaggatcagt ggaaggcctc gccgcgcgtg
     1201 caggcgcgca tgcgcgagct cggcgtgccg aacgaggagg ccctgcaggg ctatttcacg
     1261 catcgcctgg gcgagtacct cgaggcccac gggcgccgtc tcatcggttg ggacgagatt
     1321 ctcgatggcg gggtcccgcc gggcgcggcg gtcatgtcct ggcggggcgt cgaaggcgcg
     1381 attgccgcgg ccgcgagcgg acacgacacg gtgctctcac ccgcacccac gctgtatctg
     1441 gatttccgtc aaggcgccgg acccaaggaa ccgccgggca ggggcgaggt cgtctcgctc
     1501 gagcgtgtgt accgcttcga tccgcttccc gcgccactcg cggccgcagc tggccacgtg
     1561 ctcggggtgc aggccaatct gtggaccgag cacgtacgca ccgaggagtg ggcggcctac
     1621 atgacctggc cgcgtgcggc ggcgctggcg gagatcggct ggtcgcccgc ggagcggcgc
     1681 gactacgcgg acttccaacg gcgtctgccc gccgcgttcg accgctatcg cacgctcggg
     1741 atcgcctttt ccgcggatgt gttcgcaccg ccgcgcacgc tcggcgtctg ggaccggcat
     1801 atgagtcaag acctcgccag ctgtacggag aaatcgattc tgaacctcga ggatgacgcg
     1861 ccgctcgagg gagcacgcgc ggtctttttg atcgacatcg agaacccctg ctggatgatg
     1921 cccgccgccg acctgagcag cgcgcgcaaa ctgacggcgg cggtgggcca ggtgcctttc
     1981 aactttcaga tcggtcatga cgtcgacgct ataacgctgc ggcctccgcg ctcggcagcc
     2041 ggagagctcg aggtgcgtct cgatcactgc gacggtgcgc cgctggcggt gctgccgctt
     2101 gcacccgccg ccggccatga tgccgtgacg gtgcttcccg cggtggcgct gccacccctg
     2161 cagggaagcc atgcgctctg cctcaccttc acgcagcgca cactcgatcc gctgtgggca
     2221 ctcgactggg tgcagctctc gccatgaggc catccgacac gctgacgctg cttgcgccgc
     2281 tcgccggttg gagcgcgccg ctcgaggagg cacccgatga ggtgttcgcc gcgcgtctgc
     2341 tcggcgatgg cgtcgccatc gatccgacgg aggggacgct gcacgccccc tgcgacgccg
     2401 aggtcgtgtt gctgcccgcc tcacggcatg cagtgacgct gcgcactgtg gccggatgtc
     2461 ggatcctgct gcacgtcggt atcgacaccg tggcgctcgg cggggagggg ttccaggcgc
     2521 acgtcagcca gggcgcgcgg gtacgcgccg gcgacctgct cctgagcttc gacctcgact
     2581 tcctcgcgca gcgcgcaccc agtgtgctca ctccggtcat catcacatcc gacagtggct
     2641 gcgccattcg cacgcgcacc gtcgatcgcg cggtcgcact cggcgagccg ctcatggagg
     2701 tcacggtact ccaagcgctg ccggatgact cgagccgcag ccgcgcaacg gctgccgtgg
     2761 tcgcgacgcg cacgctgaag gtgagcttcg agcacggcat tcatgcccgt cccgcggcgc
     2821 tgctcgccgg ctcgctgcgc tcg
//
LOCUS       DATDMP010000116         3014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1240658, whole genome shotgun sequence.
ACCESSION   DATDMP010000116 DATDMP010000000
VERSION     DATDMP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240658"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1416
                     /locus_tag="VI195_01955"
     CDS             199..1416
                     /locus_tag="VI195_01955"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6823176.1"
                     /translation="MNPAFLSWLVHGLVSLPWWGYVVVTLAVTHVTIAAVTIFLHRGQ
                     AHRGLDLHPAVSHFFRFWLWLTTGMVTREWVAIHRKHHAFVETENDPHSPQTRGIATV
                     LLQGTELYRAEAKNRETLEKYGAGTPNDWIERNVYTPYSWQGLGVMLAIDVALFGPIG
                     LTLWAVQMAWIPVTAAGIINGLGHYWGYRNFSTDDTSTNIVPWGILIGGEELHNNHHA
                     YGTSAKLSSRWYEFDLGWTYIRTLEALGLARVRKVAPRIKFDREKLHADLETLQSVIT
                     HRYAVLSRYARTAQHALAVEYAALRERARRGELKAELAELPSVRRLRHWLQSDASKLP
                     ERDRARLATALESSKALQTLYAMRQELATLWERSTESREQLLARLQDWCRRAEASGIA
                     ALRSFSQELRTYT"
     gene            complement(1508..1663)
                     /gene="rpmG"
                     /locus_tag="VI195_01960"
     CDS             complement(1508..1663)
                     /gene="rpmG"
                     /locus_tag="VI195_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY6823177.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKKTTPDKMEVKKFDPVARRHVL
                     YKETKLK"
     gene            complement(1684..1920)
                     /gene="rpmB"
                     /locus_tag="VI195_01965"
     CDS             complement(1684..1920)
                     /gene="rpmB"
                     /locus_tag="VI195_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY6823178.1"
                     /translation="MARVCQVTGKAPIVGNNVSHANNRTRRRFMPNLQERRFWVESEN
                     RWVSLKLTNAALRTIDKKGIDAVLADMRQRGQHV"
     gene            complement(2434..>3014)
                     /gene="radC"
                     /locus_tag="VI195_01970"
     CDS             complement(2434..>3014)
                     /gene="radC"
                     /locus_tag="VI195_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY6823179.1"
                     /translation="FLRTGVAGTSAVDVGRELVARFGSLQRLFAATVDEAAGIRGFGV
                     AKYAQMKAVIELVRRALVEDIGARDSLSSPRAVCDFLLLTLGGRAYEVFMALFLDSQN
                     RLLASEELFRGTLTQTSVYPREVVKTALRHNAAGVIFAHNHPSGVAEPSRADELLTQT
                     LKSALSLVEIKTLDHFIVAGTRTVSFAERGLL"
BASE COUNT          519 a         1018 c          967 g          510 t
ORIGIN      
        1 ggctgcggga cggcacggcg gctgcgccga tcgcgtcccc gggaagccaa gcttcgcgct
       61 agctccccag gatccttgcg aaaagctgtc gagccacgcc ggaccgtgta aaattgcccc
      121 ctttgctgca aaatcgcggc gtgccggggg gtgcgccgca ccgaaacccc actgctccgc
      181 accgaaaccc cactgctcat gaatcccgct ttcctgtcct ggctcgttca cgggctcgtc
      241 tcactgccct ggtggggcta cgtggtcgtc acgctcgcgg tgacccacgt gaccatcgcc
      301 gcggtcacca tcttcctgca tcgcggccag gcgcaccgcg gtctggacct gcatccggcc
      361 gtgagccatt tcttccgctt ctggctctgg ctcaccaccg ggatggtcac ccgcgaatgg
      421 gtcgcgatcc atcgcaagca tcacgccttc gtcgagacgg agaacgatcc gcacagcccg
      481 cagacccgcg gcatcgcgac cgtgctgctg caggggacgg agctctaccg cgccgaggcg
      541 aagaaccggg agacgctcga gaagtacggc gccggcacgc cgaacgactg gatcgagcgc
      601 aacgtctaca cgccgtacag ctggcagggg ctcggggtga tgctggcgat cgacgtggcc
      661 ctgttcggcc ccatcggcct caccctgtgg gctgtgcaga tggcctggat tccggtcacc
      721 gccgccggca tcatcaacgg gctgggtcac tactggggct accgcaactt ctcgaccgac
      781 gacacgagca cgaacatcgt cccctggggc atcctgatcg gcggcgagga gctgcacaac
      841 aaccaccacg cctacggcac gtcggcgaag ctctcctcgc gctggtacga attcgatctg
      901 ggctggactt acatccggac gctcgaagct ctcgggctcg cgcgcgtgcg caaggtcgcg
      961 ccgcggatca agttcgaccg ggagaagctg cacgccgacc tggagaccct ccagtcggtg
     1021 atcacccatc gctacgcggt gctctcgcgc tacgcgcgga cggcccagca cgcgctcgcg
     1081 gtcgaatacg ccgcgctgcg cgagcgggcc cgccgcggcg agctcaaggc ggagctggcc
     1141 gagcttccga gcgtgcggcg ccttcgccat tggctgcagt ccgacgcgag caaactcccc
     1201 gagcgcgatc gggcgcgact cgccacggca ctggagagca gcaaggcgct gcagaccctc
     1261 tacgcgatgc ggcaggagct ggcgaccctg tgggagcgct ccacggaatc gcgcgagcag
     1321 cttctggcgc gcctgcagga ctggtgccgc agagccgaag cgagcggcat cgccgcgctg
     1381 cgcagcttct cgcaggagct gcgcacctac acgtgaggac gagcgggcga cgccggccga
     1441 tggccaacga aaaagcccgc cgaatggcgg gctttttgtt tgccgtcccc ggcgaagcgc
     1501 gcagctgtca cttgagcttg gtttccttgt aaaggacgtg ccggcgggcg acggggtcga
     1561 acttcttcac ttccatttta tccggcgtcg tcttcttgtt cttcgtggtc gtgtagaagt
     1621 gccccgttcc ggccgaggac tcgagcttga tcttttcgcg catggtcgct gctcctcaag
     1681 acttcagacg tgctggccgc gctggcgcat gtccgcgagc accgcgtcga tgcccttctt
     1741 gtcgatggtg cgcagcgccg cgttggtgag cttgaggctc acccagcgat tctcgctctc
     1801 gacccagaag cggcgctcct ggaggttcgg catgaaccgg cggcgggtgc ggttgttcgc
     1861 gtgggagacg ttgtttccca cgatgggcgc tttgcccgtg acttggcaga ctcgagccat
     1921 ggttgctccg atgctgtcga aggtcggccc agagccggat ggacagcgaa aaacttgtga
     1981 ttgtacgcag aaaagtctgc cgattcaacc gcttccaagg ttcaggctgg caggccaacg
     2041 ctcttacgca ggaactcgcc caaccgcctc ttgcgatcag tccttctttt tcggcggctg
     2101 cttccgctct tcagtgtgcc cgacaatcca gataccccaa gctgccacga tggcggcgaa
     2161 tacaaggacg aaaacaattc cagcgactcc cggcatgtca atcgcctctg caaaacacgg
     2221 ctccgaggac aaagcatact acgcccgaag caaacaatgc cgccacgtgc aatccgccat
     2281 actgattctg ggtgaacagg atcgacgtac ccgcgatcag gcacgccgtg gcgagattgt
     2341 ggaggaagcg gccgtaggct tcgagttgtc tacccgtcgg agtgaggaat gcctctcgca
     2401 gtgcggttaa gaggcgacga gtcatctcgc gtctcacagc agcccccgct ccgcgaacga
     2461 caccgtgcgc gtgcccgcga cgatgaagtg atcgagcgtc ttgatctcga ccagcgacag
     2521 cgcggacttc agcgtctgcg tgagcaactc gtcggcgcgg ctgggctcgg ccacgcccga
     2581 cgggtgattg tgcgcgaata tcacgcctgc ggcgttgtgg cggagcgccg tcttcaccac
     2641 ctcgcgcgga taaacgctgg tctgggtgag tgtgccgcgg aagagctcct cggaggcgag
     2701 cagccggttc tgcgaatcga ggaagagcgc catgaacact tcgtaggcgc gcccgccgag
     2761 cgtgaggagc aggaagtcgc acaccgcccg cggcgaggag aggctgtcgc gcgcgccgat
     2821 gtcctcgacc agcgcccggc gcaccagctc gatcaccgcc ttcatctgcg cgtacttcgc
     2881 gacgccgaag ccgcggatgc cggcggcctc gtccaccgtc gccgcgaaca gccgctgcag
     2941 gctgccgaag cgcgcgacga gctcgcggcc gacgtcgacc gcgctggtgc ccgccacgcc
     3001 cgtgcgcagg aaca
//
LOCUS       DATDMP010000117         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2129698, whole genome shotgun sequence.
ACCESSION   DATDMP010000117 DATDMP010000000
VERSION     DATDMP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129698"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1090)
                     /locus_tag="VI195_01975"
     CDS             complement(230..1090)
                     /locus_tag="VI195_01975"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY6823180.1"
                     /translation="MAVDVLSVTLRALSFLALFQAAGIALFLALRERQLPHTEGALQR
                     IGTLAALIATTLCLAHYALEAARMSGELGGVLDGALQGMVLHSAAGVTLSWRLLGLVL
                     IVAGLWLGGLTGRTAAIVGTVVLLASFTFIGHTSKDAVRWLLSPVLLVHLLVVAFWYG
                     SLLPLYLVSSREPPETAGLIVRQFSARAVWLVPVLFLAGLILAAALLPNLAALRRPYG
                     ELLLAKLSGFAVLMLLAAMNRWRFGPALERRDRAAGRRFRQVIGAEFVLLASVLCVTA
                     LMTTFFSPEG"
     gene            complement(1094..1465)
                     /locus_tag="VI195_01980"
     CDS             complement(1094..1465)
                     /locus_tag="VI195_01980"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEY6823181.1"
                     /translation="MTVSKWLSAGAALLLVATAHAHAHLTAAVPAEGSSGKAPEHIVL
                     SFSEAARVTALTLQRQGEEPRKLTPLPPEKAVRITVPLPKLLPGSYTLSWRVVGEDGH
                     VVPGALHFTVLEAAAGGGSGA"
     gene            complement(1503..>2013)
                     /locus_tag="VI195_01985"
     CDS             complement(1503..>2013)
                     /locus_tag="VI195_01985"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEY6823182.1"
                     /translation="KTEGLRLGDVTVDARAELLWDRIFSRWWSAQAGVRHDFGEGPAR
                     NWLALGVAGLAPYLFRIEATGYVGDAGRTALRFRAEYDLLLTQRTILQPEFELNAYGK
                     DDPERDIGAGFSDLQLGLRVRYELRRECAPYIGVAWLHRLGKTAELVRASGQDPSVIQ
                     FLAGIRFWL"
BASE COUNT          392 a          671 c          663 g          287 t
ORIGIN      
        1 ggtgccgctg aagatcaaca ggccgacgaa tgttaacgcg gcagcgcgca aaaccgcctg
       61 cttcttgcct tgcatggtgt tctccccggg gatcgtgagg cgcgaaagtt cttgttgtgc
      121 gcggcggcgc tcggagtacc gaccgcgcaa aaaaatggtg cgccccgcac gcgcggcgtg
      181 tcaattcaac tgcctcaggc cgaagcgttg cagcgatcag tacgccggcc tagccctcgg
      241 gcgagaagaa ggtcgtcatt agcgccgtga cgcacaggac gctcgcgagc agcacgaact
      301 ccgcgccaat cacctggcgg aagcggcgcc ctgcggcgcg gtcgcgccgt tccagcgcag
      361 gtccaaagcg ccatcggttc atcgccgcga gcagcatgag cacggcgaag ccggacagct
      421 ttgccagcag tagctctcca tacgggcgcc gcagcgcagc caggttcggc agcagcgcag
      481 ctgcgaggat gaggcccgcg agaaagagta ccggcacgag ccacaccgcg cgcgccgaga
      541 actgtcgcac gatgagtccg gcggtctccg ggggctcccg cgagctgacg aggtatagcg
      601 gcagcagcga gccgtaccag aatgccacca ccaacaggtg tacgagcagc actgggctca
      661 gcagccagcg caccgcgtct ttcgaggtat gcccgatgaa ggtgaatgag gcgagaagca
      721 cgaccgtccc cacgatggcg gcggtgcgtc cggttagccc tccaagccac aggccagcga
      781 cgatcagcac cagaccgagc agtcgccacg acaacgtgac gccggcggcc gagtgcaaga
      841 ccatgccctg taacgcgcca tcgagcactc caccgagctc gccgctcata cgcgcggcct
      901 ctagcgcata gtgagcgagg cacagggtgg tggcgatgag cgccgcgagc gttccgatcc
      961 gctgcagtgc gccctcggta tgcggcagct gccgctcgcg aagcgcgagg aagagagcaa
     1021 tcccggccgc ctggaagagc gccagaaagc tcagcgctcg cagagtgacc gagagaacat
     1081 cgacagccat ttctcaggcg ccgctcccgc cgccggcggc cgcttcgagg acggtaaagt
     1141 gcagtgctcc cggcaccaca tggccatcct cgccaacgac ccgccagctg agggtgtaac
     1201 tgccgggaag cagcttggga agcggcacgg tgatgcgcac cgccttctcc ggtggtaagg
     1261 gtgtgagttt gcgaggctct tcaccctgcc gttgcagggt cagggcggtg acgcgtgccg
     1321 cttccgagaa gctcagcacg atgtgctcgg gcgccttgcc ggagctgccc tcggccggaa
     1381 ccgcagcggt gagatgcgca tgtgcgtggg cggtggcgac caacagtaac gctgcgcctg
     1441 cggatagcca cttcgatacg gtcatggacg agtctcccaa tgtctgacgc cactggcggc
     1501 gctcacaacc agaagcggat gccggcaagg aactggataa cgctcggatc ctgccccgag
     1561 gcacggacca gctccgcggt cttgccgagg cggtgcaacc aggcgacccc gatgtacggg
     1621 gcacactcac ggcgcagctc ataacgcact cgcaatccga gctgcagatc cgagaagccc
     1681 gcgccgatat cccgctccgg atcatcctta ccgtacgcgt tcagctcgaa ctccggttgg
     1741 agaatcgtgc gttgcgtaag gagcaggtca tactcggcgc gaaagcgtag cgccgtgcgc
     1801 ccggcatcgc ccacgtagcc ggtggcctcg atccggaaaa gatagggcgc caagcctgcg
     1861 acgccgagcg cgagccagtt gcgggcaggg ccctctccaa aatcgtggcg cacgcccgcc
     1921 tgcgcgctcc accagcggct gaagatgcgg tcccacagga gctctgcgcg tgcatccacc
     1981 gtgacatccc cgaggcgcag gccttcggtc ttg
//
LOCUS       DATDMP010000118         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_18883, whole genome shotgun sequence.
ACCESSION   DATDMP010000118 DATDMP010000000
VERSION     DATDMP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18883"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VI195_01990"
     CDS             complement(<1..1709)
                     /locus_tag="VI195_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6823183.1"
                     /translation="MKVIGKPRRRVDGRAKVTGLTRFADDVVLPRMLHMKLLRSPHPH
                     ALIESIDVSRAVAYAGVHLVLTGKDFPASFGILPITQDEYPLAPEHVRYIGDPVAAVI
                     ARDEQTACEALELIEVRYRPLDTIASPEEALAKPEPRLHAYSERGNIHRLQAFQFGDA
                     DAAIAKSDYVFEDLFFYEGNTHLPIEQHACVAAPDADGKLTLWSSTQVPHYVHRALAR
                     VLQMPASQIRVIATPNGGGFGGKCDVCNHEMVAGKAAMVLGRPVKICLTREEVFYMHR
                     GRHPVLMKFRTGVSKDGKLTGMHLQTLVDGGAYGSYGVASTFYTGALQTVTYELPRYQ
                     FEAARVFTNKPPCGPKRGHGTPQPRFGQEIQLDKIAERLGRDPAELRLGMLAKPNSLT
                     ANWLKIGSIGLEQCIRAVVERSDWKNRFRKLGTGRGLGLACGSYLCGAGLPIYWNKMP
                     QSGVQILLDRSGQVTVFCGATEIGQGSDDVLAAIVAEVLGVDPQEVRCITGDTGITPV
                     DLGSYSSRVTVMMGNAAIQAAQKLKAMLAEAAAEELEVVPDRLQFANGRVAFDSRSLT
                     FAEA"
     gene            complement(1693..2178)
                     /locus_tag="VI195_01995"
     CDS             complement(1693..2178)
                     /locus_tag="VI195_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6823184.1"
                     /translation="MSRAEITLSVNGEAVEVTFVPYKTLLELLREDLALTGTKHGCEL
                     GECGACAVLIDGEPQLSCLVLALECAGRSVETVEGLANGAELHPLQAAFADLGAAQCG
                     YCTPGILLTAKALLAHEPQPSRSRIQEAISGNLCRCTGYQQIVEAIEEGARRMADESH
                     R"
     gene            2338..>2505
                     /locus_tag="VI195_02000"
     CDS             2338..>2505
                     /locus_tag="VI195_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY6823185.1"
                     /translation="MTLRIGDTAPNFQADTTEGRIDFHQWIGDKWAVLFSHPKDFTPV
                     CTTELGTMARMK"
BASE COUNT          422 a          832 c          826 g          425 t
ORIGIN      
        1 gcctcggcga aggtcaagct gcgtgaatcg aacgcgacgc ggccgttggc gaattgcagg
       61 cggtctggca cgacctcgag ctcttccgcg gccgcctcgg cgagcatcgc cttcagcttt
      121 tgcgccgcct ggatcgcggc gttgcccatc atcaccgtga cccggctcga gtaggagccg
      181 agatcgaccg gcgtgatgcc ggtgtcgccg gtaatgcagc gcacttcctg cggatcgacg
      241 cccagcacct cggcgacgat cgcggccagc acatcgtcgg agccctggcc gatctcggtg
      301 gcgccgcaga agaccgtgac ctgcccgctg cgatcgagca ggatctgcac gccgctctgc
      361 ggcatcttgt tccagtagat cggcagaccg gcgccgcaca ggtaggagcc gcaggcgagc
      421 ccgagaccgc gtccggtccc cagcttgcgg aagcgattct tccaatcgga acgctcgacc
      481 accgcccgga tgcactgctc gagaccgatc gagccgatct tcagccagtt ggcggtaagt
      541 gaattgggct tcgccagcat gccgagccgc aactccgccg gatccctgcc gaggcgctcg
      601 gcgatcttgt cgagctggat ctcctggccg aagcgcggtt gcggcgtgcc gtggccacgc
      661 tttggaccgc agggcggctt gttggtgaac acacgggccg cttcgaactg gtagcgcggc
      721 agctcgtagg tcaccgtttg cagtgcaccg gtgtagaaag tgctggcgac gccataggag
      781 ccgtaagcac cgccatcaac cagggtctgc aggtgcatgc cggtgagctt gccgtccttg
      841 ctcacgccgg tgcggaattt catcagcacc ggatggcgcc cgcggtgcat gtagaacact
      901 tcttcgcggg tgaggcagat cttcaccggc cggccaagca ccatcgccgc cttgccggcc
      961 accatctcgt ggttgcacac atcgcacttt ccgccgaagc cgccaccatt gggcgtcgcg
     1021 atgacgcgga tctgcgaagc cggcatctgc agcacgcgcg cgagcgcgcg atgcacatag
     1081 tgcggcacct gcgtcgagga ccagagcgtg agcttgccgt cggcgtcggg cgcggcgacg
     1141 caggcgtgct gctcgatcgg cagatgggta ttgccctcat agaagaacag gtcctcgaac
     1201 acgtaatccg acttggcaat cgccgcatcg gcatcgccga actggaacgc ctgcaggcgg
     1261 tggatgttgc cgcgctcgct gtaggcatgc aggcgcggct cgggctttgc cagcgcctcc
     1321 tcgggcgagg cgatggtgtc gagcggccgg tagcgcactt cgatcagctc gagcgcctcg
     1381 cacgcggtct gctcgtcgcg cgcgataacc gccgcgaccg ggtcgccgat gtagcggacg
     1441 tgctcgggcg cgagcggata ttcgtcctgc gtaatcggca ggatgccgaa cgaggcgggg
     1501 aaatccttgc cggtaagaac gagatggacg ccggcgtagg cgacagcgcg cgagacgtcg
     1561 atcgattcga tgagcgcgtg cgggtgcggc gagcgcagca gcttcatgtg cagcatgcgc
     1621 ggcagcacga catcgtcggc gaagcgcgtc agtcccgtga ccttggcgcg tccgtcaacg
     1681 cgccggcgcg gcttacctat gactttcatc ggccattctc ctcgcgccct cttcgatcgc
     1741 ctcgacgatc tgctgatagc cggtgcagcg gcaaaggttg cccgagatcg cttcctggat
     1801 gcggctgcgc gagggctgcg gctcgtgcgc gagcagcgcc ttggccgtga gcaggatgcc
     1861 gggcgtgcag tacccgcact gcgcggcgcc aaggtcggca aaggccgcct gcagcggatg
     1921 cagctccgct ccgttcgcca gaccttccac cgtctccacc gagcggccgg cgcattcgag
     1981 cgccagcacg aggcacgaga gctgcggctc gccgtcgatc agcacggcgc aggcgccgca
     2041 ttcgccgagc tcgcagccgt gcttggtgcc ggtaagcgcc agatcctcgc gcagcagctc
     2101 gagcagggtc ttgtaaggca cgaacgtgac ttcaacggct tcgccattga cggagagcgt
     2161 gatctcggcg cggctcattt gttggggccc caaactaatt cgcccactct actccccgct
     2221 acggcatgcc gcaaagctgc gagcgtattc cgcagcgggg gcgcggttat tcgaaaaaga
     2281 tcgctccgcg gcacgccagt ggcgctacca tttcgccccc atatccaagg agccctcatg
     2341 accctgcgta tcggcgatac cgcgcccaat ttccaggcag acaccactga agggcgcatc
     2401 gatttccacc agtggatcgg cgacaagtgg gcggtgctct tctcgcaccc caaggatttc
     2461 accccggtgt gcacgaccga gctcggcacg atggcgcgca tgaag
//
LOCUS       DATDMP010000119         3495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_796603, whole genome shotgun sequence.
ACCESSION   DATDMP010000119 DATDMP010000000
VERSION     DATDMP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796603"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VI195_02005"
     CDS             complement(<1..1240)
                     /locus_tag="VI195_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823186.1"
                     /translation="MSQLLRRLVVLQPGEAPALLASFATLLCTFSSYTILRPVRDALG
                     ITSGLEKIPYLFWGVFVVMLLLQPVWGWLTSRYPRTVFLPWVYGFFAFNLVGFYLWFH
                     FQADHTWIARTYFVWVSVFNLFVVAAFWSLMADVFTREQAGRVFGFIWAGASTGGLIG
                     PALAHELAVPFGAINLLPISAGLLLLALVFMAQVIRWQRAQRGVAAPASAGAAPPART
                     ESALGGGTFAAFTQVMRSPYLLGIAVFVLLMTWVSTFLYLEQQAFVAKVFTSADQRTR
                     FFAGIDFWVQAASLTMQLLLFGRLFKWVGLRAMLTAMPLIMTAGYAIFALAPRFMVLV
                     VVYAVRRVGDYALTRPCRDALFTVVSREEKYKAKSLIDTFVYRGGDATSGSLYQALTI
                     SLGAGPSAVGWVGATISALWT"
     gene            1475..1594
                     /locus_tag="VI195_02010"
     CDS             1475..1594
                     /locus_tag="VI195_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEY6823187.1"
                     /translation="MHAHRVQSAYGDNYPRLAALKKRYDPANLFRLNANIKPA"
     gene            1711..2634
                     /locus_tag="VI195_02015"
     CDS             1711..2634
                     /locus_tag="VI195_02015"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6823188.1"
                     /translation="MDIDALPIARLERETIRALSRRSDRYGALQLALHLGLLVATGAL
                     LWATRGHAWFAAALVLHGIVLCFLFCALHECIHRTAFASRYLNDGVAWACGALLVLPP
                     EYFRLFHFAHHRHTQNPARDPELALTPPASRAAYWWRVSGLPYWRERLQTSLRHALTG
                     RVTEPFVPAARAPAVVREARLLWGGYLAIAAGSIWLHSAAALLYWVVPALLGQPFLRL
                     FLLAEHSGCALAEDMLANTRTTYTNLAVRLLSWRMSYHAEHHSFPSVPFHALARVNAL
                     LAADGRVTSPGYVAVHRALLRGLRAPRAAPR"
     gene            complement(2689..>3495)
                     /locus_tag="VI195_02020"
     CDS             complement(2689..>3495)
                     /locus_tag="VI195_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY6823189.1"
                     /translation="LREFAGHNQGTIVVPAVLAGAMGPVTPAGCLAEILAETLAGMAL
                     TQLVRPGAPVILGSFVGAISMRTGAPTFGTPEATQMIFATAQLARRLRIPCRSGGSLC
                     ASKVADAQAAYESAHTLLPTLLAGVNLVTHAAGWLEGGLVASYEKFVMDADQCAMMQM
                     LAQGMDLSERGQALDAIREVGPGSHFLGCAHTLANFESAFYQSSIADYSSYEQWTLEG
                     KLTAEQRANRVWKKMLAAYQDPGIDAAQDEAMLDFIARRKAVLSDTIDDE"
BASE COUNT          596 a         1230 c         1149 g          520 t
ORIGIN      
        1 gcgtccacag cgcagagatc gtcgctccca cccaaccgac tgcggacggt ccggcgccga
       61 gggaaatggt gagcgcctga tagagcgagc cgctggtggc atcgccaccc cgatacacaa
      121 aggtatcgat caggctcttg gccttgtact tctcctcgcg gctcaccacg gtgaacagcg
      181 cgtcccggca gggccgcgtg agcgcgtagt caccgacgcg ccgcaccgca tacaccacca
      241 cgagcaccat gaagcgtggc gccagcgcaa agatcgcgta gccggcggtc atgatgagcg
      301 gcatcgcagt cagcatcgcc ctgagcccca cccacttgaa caggcggccg aacagcagca
      361 gttgcatcgt gagcgatgcc gcctgcaccc agaagtcgat accggcaaag aagcgcgtgc
      421 gctgatccgc gctggtgaac accttggcga cgaaggcctg ctgctcgaga tagaggaatg
      481 tcgagaccca cgtcatcagc agtacgaaca cggcgatgcc gagcaggtaa ggcgagcgca
      541 tcacctgcgt gaatgcggca aaggtgccac cgccgagcgc gctttccgtg cgcgcaggcg
      601 gcgcggcgcc cgcactggcc ggcgcagcca cgcctcgttg ggcgcgctgc cagcgaatca
      661 cctgcgccat gaacaccagc gcgagcagca gcagccccgc cgagatcggc aacaggttga
      721 tggcgccgaa tggcaccgcg agctcatgag cgagggccgg gccgatcagc ccaccggtgc
      781 tcgcccccgc ccagatgaag ccgaacaccc gcccggcctg ctcgcgggta aagacatcgg
      841 ccatgaggct ccagaatgcc gccaccacga acaggttgaa gacgctcacc cacacgaagt
      901 aggtgcgcgc gatccaggtg tggtcggcct ggaagtggaa ccacaggtaa aagcccacca
      961 ggttgaatgc gaagaaacca tacacccacg gcaggaatac cgtgcgcggg taacgcgagg
     1021 tgagccagcc ccacaccggc tgcaacagca gcatcaccac gaacaccccc cagaacaggt
     1081 agggaatttt ctccagcccc gaggtgatgc ccagggcatc gcgcaccggc cgcaggatcg
     1141 tgtagctcga gaaggtgcac agcagcgtcg cgaaggacgc gagcagtgcc ggcgcctcac
     1201 ccggctgcag caccaccagg cgacgaagga gttggctcat ggcgcggcac ctcggtgttg
     1261 tggccgtcga caaccgtcag caaggcgccg tagtgtgcca cagccgccgg cgcggcagat
     1321 ggcgtcaggg tgcggcgaat ccagtcaaac ccaatcccta cggcgcctac ttcaagggcg
     1381 gcctcgcgcg cgagctcact ccagcgctca tcgacgccac ggtcgactac ctcgaggcag
     1441 caccgctgcc gatctcggtg aatcgcagca ggatgtgcac gcccatcgag tccagagcgc
     1501 gtacggggac aactacccgc gcctggccgc gctcaagaaa cgctacgacc cggccaatct
     1561 cttccgcctg aacgccaaca tcaagccggc ctgacatccc tgctcatggc cgcgccgggg
     1621 tgggcgcagc ctgttgattt tgcgggcgcc gcgcggcggc gctgtcggcc gccggccggg
     1681 cggccttaaa atggcgtgcg gacagccgcc gtggacatcg acgcactgcc aattgcgcgc
     1741 ctcgagcgcg agacgatacg agccctgtcg aggcgctcgg atcgctacgg cgcgctgcag
     1801 ctcgccctac atctcgggct gctcgtggcc acgggcgcac tactgtgggc gacccgcggg
     1861 cacgcgtggt tcgctgcagc gctcgtgctg catggcatcg tgctctgctt cctgttctgc
     1921 gcactgcacg agtgcatcca tcgtaccgcc ttcgccagcc gctacctgaa cgatggcgtc
     1981 gcttgggctt gtggcgcact gctggtgctg ccgcccgagt acttccggct gttccatttc
     2041 gcccatcacc gtcatacgca gaacccggcg cgcgatccgg agctcgcgct gacgccgccg
     2101 gcgagccgcg ctgcctactg gtggcgggtt tccggcctgc cctactggcg cgagcggctg
     2161 cagacgagct tgcgccacgc actcaccggc cgtgttaccg agcctttcgt gcctgcggca
     2221 cgcgcgccgg cggtggtgcg cgaagcgcgc ctgctgtggg gcgggtacct ggccatcgct
     2281 gccggctcga tatggctgca cagcgccgct gcactcctct actgggtcgt gcccgcgctt
     2341 ctcgggcagc cgttcctcag gctgtttctt ctcgccgagc acagcggctg cgcgctcgcc
     2401 gaggacatgc tcgccaacac gcgcaccact tacaccaacc tggcggtgcg gctgctcagc
     2461 tggcgcatgt cctatcacgc cgagcaccat agtttcccct cggtgccctt tcatgccctg
     2521 gcgcgggtca acgcactgct ggcggccgac gggcgcgtga cctcgcccgg ctatgtggcc
     2581 gtgcatcggg cgctgctgcg cgggctgcgc gcaccgcgcg ccgcgccacg ctagtgcgcc
     2641 ccgcagcgct cgtcgccgcg ggccgcgggc cgtggcgcgc aggcgcgatc attcgtcatc
     2701 gatggtgtcc gacagcacgg ccttgcggcg tgcgatgaaa tcgagcatgg cctcatcctg
     2761 cgccgcatcg atgccgggat cctggtaggc ggccagcatt ttcttccaca cgcggttggc
     2821 gcgctgctcg gcggtgagct tgccctcgag cgtccactgc tcatacgaag agtagtcggc
     2881 aatgctcgac tggtagaagg cgctctcgaa gttcgcgagc gtatgcgcac agccgaggaa
     2941 gtggctgccg gggccgacct cgcggatcgc atcgagcgcc tggccacgct cgctgagatc
     3001 catgccctgc gcgagcatct gcatcatggc gcactgatcc gcgtccatga cgaacttctc
     3061 gtaactcgcg accaggcctc cctcgagcca gccggccgcg tgcgtcacca ggttcacgcc
     3121 ggcgagcagc gtcggcagca gcgtgtgcgc gctctcgtag gcggcctgcg catcggcgac
     3181 cttggaggcg cacagcgagc cgccgctgcg gcaggggatg cgcagccgcc gcgccagctg
     3241 cgcggtcgcg aagatcatct gcgtcgcctc gggagtgccg aaggtcggcg ccccggtacg
     3301 catcgagatg gcgccgacga aggagccgag gatgaccgga gcgccgggac gcaccagctg
     3361 cgtgagcgcc atgccggcga gggtctcggc gagaatctcg gccaggcagc cggcgggagt
     3421 caccggcccc atggcgcccg cgagcactgc gggtaccacg atcgtgccct ggttatggcc
     3481 ggcgaattcc cgcag
//
LOCUS       DATDMP010000120         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1462892, whole genome shotgun sequence.
ACCESSION   DATDMP010000120 DATDMP010000000
VERSION     DATDMP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462892"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VI195_02025"
     CDS             <1..236
                     /locus_tag="VI195_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY6823190.1"
                     /translation="VKLWFTQELEPAFSTVRVLDKSGKPVDGGGGRVDKNDHTVLEVP
                     LPPLAPGTYRVRWRVVSVDTHVTEGDFTFDVAP"
     gene            240..1574
                     /gene="copD"
                     /locus_tag="VI195_02030"
     CDS             240..1574
                     /gene="copD"
                     /locus_tag="VI195_02030"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HEY6823191.1"
                     /translation="MQSLMAATRAVHFAALIALFGEFVFLIWVADATVRGDEWAALAQ
                     RAVRMAGWSITVAVLSGVLWLGLEAAEMSGAGAAAAWDGPTLGMVIIDTRFGHAWSVR
                     LVLALAIALVLTLARATIGRSRPLARTVALLSGVLLATVAWAGHAAGERGTDRIVHLS
                     ADAMHLAAAGAWLGALPSFASLLANASKASTPEFAGIAARRFSTLGVVSVGTLVITGI
                     VNAWYTVGSMPALVATPYGRLLLLKLLLFGAMLALAAINRLWLTRRLTAGRDAWAARQ
                     LQRNAIAETALGVAVLAVVGVLGITTSAAHVYAEPMSGHARAAFGRTADPREATRTIR
                     IEMGDDMRFMPADIRVKRGEVVRFLVENKGKLMHEMVLGTLDELQAHAAMMGQHPDME
                     HDAQPAPNELQVGPGKSGELGWQFTAAGAFYYGCLVPGHLEAGMIGKVLVTN"
BASE COUNT          382 a          733 c          753 g          442 t
ORIGIN      
        1 cggtgaagct gtggttcacg caggagctcg agccggcgtt cagcacggtg cgcgtgctcg
       61 acaagagtgg caagccggtc gatggcggag gtgggcgcgt cgacaagaac gaccatacgg
      121 tgctggaagt tcccctgccg ccgcttgcgc cgggcactta ccgcgtgcga tggcgcgttg
      181 tctcggtcga cacgcacgtc accgaaggtg atttcacctt cgacgtggcg ccctgagcga
      241 tgcagtcctt gatggccgcg acgcgcgccg tgcatttcgc cgcgctcatc gcgttgttcg
      301 gggaattcgt ttttctcatc tgggtcgctg acgccacggt gcgcggcgac gaatgggccg
      361 cactcgcaca acgcgctgtg cgcatggccg gctggtcaat caccgtcgca gtcctgtccg
      421 gcgtcttgtg gctgggcctc gaagccgccg agatgagcgg cgccggagcg gcggccgctt
      481 gggacggacc caccctggga atggtgatta tcgacacccg attcgggcac gcatggagcg
      541 tgcgtctggt tctggcgctt gccatcgcgc tggttcttac gctcgcccgc gcaacaatcg
      601 gccgttcgcg gccgctcgcc cggacggtcg ccttgctgag cggtgtgctg cttgcgacgg
      661 ttgcatgggc tggtcatgcg gccggcgaac gcggcacgga tcgcatcgtc catctttcgg
      721 ccgacgcaat gcatctcgcc gccgcaggcg cctggctcgg cgcgctcccg agtttcgctt
      781 cgcttctcgc gaatgcctcg aaggcctcga cacccgagtt tgcgggcatt gcagcgcggc
      841 gattttccac gctcggcgtc gtgagcgtcg gcacactggt gatcacgggg atcgtgaacg
      901 cgtggtacac agtgggcagc atgccggcgc tggtcgcgac tccgtacggg cgcttgctgc
      961 tcctcaagct cctgctgttc ggcgccatgc ttgcccttgc cgcgatcaac cgcctgtggc
     1021 tcacccggcg cctcacggcc ggcagggacg cctgggctgc acggcagctg cagcgcaatg
     1081 ccattgccga gaccgctctg ggagttgcag tgctcgccgt cgtcggcgta ctcggcatca
     1141 ccacgtcagc ggctcacgtc tatgccgagc caatgagtgg ccatgcgcgg gcggcattcg
     1201 gccgcaccgc cgatcctcgc gaggcgacgc gcaccatccg catcgagatg ggggacgaca
     1261 tgcgtttcat gccggcggac atccgcgtca agcgcggtga agtggtgcgg ttcctagtgg
     1321 aaaacaaagg caagctgatg cacgagatgg tgctcggcac gctggacgag ctccaggcgc
     1381 acgccgcgat gatgggtcag catcccgaca tggagcacga cgcccagccc gcacccaacg
     1441 agctccaggt cggtcctggc aagtccgggg agctcggctg gcaattcacc gcagcggggg
     1501 cgttctacta cggttgcctc gtcccggggc acctcgaggc aggcatgatc ggtaaggtcc
     1561 tcgtgaccaa ctaacggcga ttgcgcttca cctgacgccc accacttccg atcctgatcg
     1621 gatgtccgct cgtcagcgtg gcgcgaagcc gaggtccttc tctccttgac accgcgatcg
     1681 gcgaagccgc gtggagcttg ccactgcgtc cagtgcagga agcagactgg caactttttg
     1741 gtcgcttaac gtccgccgca ccgcagtcgg gttcgctgga aatgaacacg taaagtggtt
     1801 ccagtccagt cctcggagaa ggttcagcga ggactctgtc cgtttgcgca tctacgctct
     1861 tgcggtagcc ctcgatcgag ggctaccaat ggacgagcgt cgcgctcgga tgcttggaca
     1921 catctacgga gctcaagatg gctcggacgg catgaacgct gataatcacg ccgatcgccc
     1981 gacctcggac attggcggtc attcaaggcg ttttccagtc cagatgtaaa ttgcgttgac
     2041 gaaaatggaa attgagcgca acgtgtagac gcaagtcgtt cagtaattcc ttccgcggag
     2101 ccaaatcgga atccttccga tcacgacgcc caagggcctt taccgaaagg cgagatgggc
     2161 gtctgggcta ctcgacagcg cacggtgctg cgtgcgtgcg ccattctcgg cgacgagcta
     2221 gcgctcgcca ggcatcttgg cgttcccgcg tcgcaggtag tcgattggat actcggggac
     2281 gtaccggtgc catcggacaa ctttctgcag
//
LOCUS       DATDMP010000121         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1907191, whole genome shotgun sequence.
ACCESSION   DATDMP010000121 DATDMP010000000
VERSION     DATDMP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907191"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VI195_02035"
     CDS             complement(<1..226)
                     /locus_tag="VI195_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823192.1"
                     /translation="MRLAFRFLVGSWLLVSALAPAAGAAPKPAAAEPSPAIAHEAESA
                     VVKVFSTMRRPDVARPWSKASPIEAFGSGVV"
     gene            414..1181
                     /locus_tag="VI195_02040"
     CDS             414..1181
                     /locus_tag="VI195_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823193.1"
                     /translation="MKRALIATRAAIAATASLLIPVLGVSPASAAQPIPGYISSAVAD
                     SARPAEDQQRDAERKPAETLVFAGVKPGETVIEIAPGKGYYTRLLSAAVGAKGKVYAA
                     TSQPKPDAPPPPVQAIAADPHYSNVSVSFQRVADLKLPPGADLVWTSLNYHDFHNIPD
                     VDVATINRAIFAALKPGGTYLVVDHAAAAGSGTRDTNTLHRIDEETVKQEVTAAGFRL
                     AGESEVLHNKADPHTAKVFDAEIKGHTDQFMLKFKKP"
     gene            complement(1214..1672)
                     /locus_tag="VI195_02045"
     CDS             complement(1214..1672)
                     /locus_tag="VI195_02045"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEY6823194.1"
                     /translation="MSPTLDPDPYHLDRFVEAQDAVFGRVCAELAAGHKHSHWMWFIF
                     PQLRGLGSSAMAERYAIGSLDEARAYLAHPILGSRLRRCTELVNGLEGRSAQEVFGHP
                     DQLKFRSSMTLFARAAAGPNEPFAKALGKYFAGEEDPLSRELLDNRSIPR"
     gene            complement(1669..>2368)
                     /locus_tag="VI195_02050"
     CDS             complement(1669..>2368)
                     /locus_tag="VI195_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6823195.1"
                     /translation="AWADRYSDAVAHEGRPADDLKRDEIDHPAEVLRLSGIRAPMVVA
                     DFLAADGYYSELLSYLVGPEGKVYLLNNDAYDKWSENQWQGRTARLPNVVHRTIAVEH
                     LELPARSLDAILVIKVYHDLYWHADQGPWPKDINPDAVLTEIARVLKPGGTLLLVDHS
                     AKPGTGSSVAGSLHRIDEQYARRDFEKHGFELVGKSDVLRRPDDPRELITYKGPMVGK
                     TDRFAMVFRKKGST"
BASE COUNT          433 a          769 c          783 g          383 t
ORIGIN      
        1 tgaccacacc ggagccgaag gcctcgatcg gagaggcttt gctccatggc cgcgccacat
       61 ccggacggcg catggtcgag aacaccttga ccacggcgct ctccgcttcg tgcgcgatcg
      121 ccgggctggg ctcggccgcg gcgggcttgg gggcggcgcc ggcggcgggt gcaagtgcgc
      181 tcacgagcag ccagctgcca accaggaacc ggaacgcaag ccgcatgaat ggctttcctt
      241 tgcaattttg gacggcgaat tctgacacag aagctcaagc ttgccctgag agccgagaca
      301 cgcggcgcgc tgtgccgatc gccgggcgcg ccagggcgag gccgccgccg gatggctcct
      361 gatacagtgg cgcgccgccc tcagctcaga gcggtatggg taggagcgac tccatgaaac
      421 gtgcactcat cgcgacgcgg gcagccattg ctgcgacggc gagcctattg attccggttc
      481 tcggggtatc gccggcctcc gcggcacaac cgattccggg gtacatcagc agcgccgtcg
      541 ctgacagcgc ccgccctgcg gaggaccagc aacgcgacgc cgagcgcaaa cccgccgaga
      601 cgctggtgtt tgccggagtg aagcccggag agacggtgat cgagatcgct ccaggcaagg
      661 gctactacac acgcctcttg agcgcagcgg taggagccaa gggcaaggtg tatgcggcca
      721 cctcgcagcc gaagcccgac gctccgccgc ccccggtgca ggccatcgcg gccgacccgc
      781 actacagtaa tgtgagtgtc tctttccagc gcgtcgcgga cctgaagctg ccgccgggcg
      841 cggatctggt gtggacgtca ctgaactatc acgacttcca caatatcccc gatgtggatg
      901 tggccacgat caaccgcgcg atcttcgcgg cgttgaagcc gggcggcacc tacctggtgg
      961 tcgaccacgc ggccgcggcc ggctcaggta cgcgggatac caacaccctg caccgcatcg
     1021 acgaggagac ggtaaagcag gaggtgacgg cggccggctt caggctggcc ggcgagagcg
     1081 aggtcctgca caacaaggcc gatccgcaca ccgcgaaggt cttcgacgcc gagataaaag
     1141 gccacactga ccaattcatg ctgaagttca agaagccctg atagagcacc ccaggaaatc
     1201 acgcttgccg cgatcagcga ggaatcgacc ggttgtcgag tagctcgcgg ctgaggggat
     1261 cctcctcacc cgcgaagtat ttgccgagcg ctttcgcaaa gggctcgttc ggtcccgccg
     1321 cggcccgcgc gaacagcgtc atggaggagc ggaacttcag ctgatccggg tggccgaaga
     1381 cttcctgcgc gctgcgaccc tcgagcccat tgacgagctc ggtgcagcgg cgcagccggc
     1441 tcccgaggat cgggtgggca agataggcgc gcgcctcatc gagtgaaccg atggcgtaac
     1501 gctccgccat ggcgctcgag cccaatccgc gcagctgcgg gaatatgaac cacatccagt
     1561 ggctgtgctt gtgacctgcg gcgagctcgg cgcagacgcg gccaaacacc gcatcctgtg
     1621 cctccacgaa gcgatcgaga tggtacggat cggggtcgag cgtagggctc atgtcgagcc
     1681 cttcttgcgg aacaccatcg cgaaccggtc tgtcttgccg accatcgggc ccttgtaggt
     1741 gatcagctcg cgcggatcat cgggacggcg cagcacatcg ctcttgccca cgagctcgaa
     1801 gccatgcttc tcgaaatcgc gccgtgcgta ctgctcatcg atgcgatgca ggctcccggc
     1861 aacggaactg cccgtgccgg gcttggccga atgatcgacc agcagcaggg tgccgccggg
     1921 cttgagcacg cgggcgatct cggtgagcac ggcatccgga ttgatgtcct tgggccaggg
     1981 gccctggtcg gcatgccagt agagatcgtg atagaccttg atcaccagga tcgcatcgag
     2041 cgagcgcgcc ggcagttcca ggtgctcgac ggcgatggtc cggtgcacga cgttcggcag
     2101 ccgtgcggtg cgcccctgcc actggttctc gctccacttg tcataggcgt cgttgttgag
     2161 caggtacacc ttgccctcgg gacctaccag gtagctgagg agctcgctgt agtaaccgtc
     2221 ggcggccaga aagtccgcga cgaccatggg cgcgcggatg cccgaaagtc gcagtacctc
     2281 agcggggtga tcgatctcgt cgcgcttcag atcatccgcg gggcgtccct cgtgtgcgac
     2341 ggcatcgctg tatcgatcgg cccacgct
//
LOCUS       DATDMP010000122         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_241187, whole genome shotgun sequence.
ACCESSION   DATDMP010000122 DATDMP010000000
VERSION     DATDMP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241187"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VI195_02055"
     CDS             complement(<1..1308)
                     /locus_tag="VI195_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY6823196.1"
                     /translation="MGVAKVACRAQLGLHAPLVQVEVSLASGLPSFCIVGLPATVIKE
                     SKERVRAALTNSGFSFPAGRITVSLAPAELPKEGGRFDLPIALGILLASGQLRASESA
                     GPREFYGELGLTGELKPVRGLLLAAVHARREAHELIVPHGNAAEASAAAPAQVHAAGH
                     LLEVCAHVSGAKRLHGHVALTRKVAPCAEVSRLELADVLGQWHAKRALVIAAAGAHSL
                     LLIGPPGAGKTMLAQRLPGLLPPLAESAALEVAAIAAVSAAGFAPGQYGQRPFRAPHH
                     TSSIAALVGGGVRARPGEVSLAHHGVLFLDELPEFARAVLEALREPLESGSVAVSRAA
                     LQNRYPAQFQLVAAMNPCPCGRHGDDAAECRCTPAQIRNYRARISAPLLDRLDLQVEV
                     PRLAACDFAAAASGARESTAAAAARVARARSLQLARQGVANAWL"
     gene            complement(1336..1623)
                     /locus_tag="VI195_02060"
     CDS             complement(1336..1623)
                     /locus_tag="VI195_02060"
                     /inference="COORDINATES: protein motif:HMM:NF016280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY6823197.1"
                     /translation="MDAFRIDELARRLLERVPPALRSMQADLESNFRAVLRERLSQLD
                     LVSRDEFDAQTRVLERTRAQLEALEARLTALEGSSGIAAPKAPGSGGAPAV"
     gene            1847..2392
                     /locus_tag="VI195_02065"
     CDS             1847..2392
                     /locus_tag="VI195_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823198.1"
                     /translation="MSILATPLRQSACLTAALAALAPGLAAAAEAPLPSKGDTAWMLT
                     ATALVLLMSVPALALFYGGLVRTKNMLSVLMQVFVGFSLITVLWCVYGYSLAFTEHNA
                     IIGGFNRLFLNGTFDAKSGTLALASTFSKHTPMYELVYVAFQATFAAIEKLPSAGLNV
                     PLRNARDNPPKNALPSVNATL"
BASE COUNT          388 a          860 c          791 g          378 t
ORIGIN      
        1 cagccaggca tttgcaacgc cttggcgcgc gagctgcagg ctgcgcgctc gtgccacccg
       61 tgccgcggcg gctgccgtac tctcgcgcgc cccgctcgcg gcggccgcga agtcgcacgc
      121 cgcgaggcgc ggtacttcca cctgcaggtc gagacggtcg aggagcggtg cggaaatgcg
      181 ggcgcgatag ttacgaatct gcgccggtgt gcagcggcac tccgcggcgt catctccgtg
      241 gcgtccgcag gggcacggat tcatggcggc caccagctgg aactgcgcgg gatagcggtt
      301 ctgcagcgct gcgcgcgaga cggcaacact gccgctctcg agcggctcgc gcagcgcttc
      361 cagcaccgcg cgcgcgaact ccggcagctc atcgagaaac aacactccgt ggtgcgcgag
      421 gctcacctct ccgggccggg cgcgcacccc gccgccgacc agcgcggcaa tcgagctggt
      481 gtggtgcggt gctcggaacg gccgctgccc gtactgtccc ggcgcgaagc cagccgcgct
      541 caccgccgcg atggcggcaa cttcgagcgc tgcactctcg gcgagtgggg gcagcaggcc
      601 cggcagacgc tgcgcaagca tggtcttgcc cgcgcctggc ggaccgatca gcagcaggct
      661 gtgggcaccc gcggccgcga tgacgagcgc acgcttcgca tgccactgcc caagcacatc
      721 cgccagctcg agccgggaca cctcggcgca cggtgcgact ttccgggtga gcgccacgtg
      781 accgtgcaag cgcttcgcac cgctcacgtg cgcgcacacc tccagcagat ggccggcagc
      841 gtgcacctga gcgggggccg ccgcgctcgc ctcggcggcg ttgccatgcg gcacgatgag
      901 ctcgtgagcc tcccggcgcg catgcacggc ggcgagcagc agtccccgca ccggcttgag
      961 ctccccggtg agtccgagct ccccatagaa ctcgcgcgga ccggcgctct cggacgcccg
     1021 cagttgtccg gaggcgagca ggatgccgag tgcgatgggt agatcgaagc ggccgccttc
     1081 cttcggcagc tccgccggag cgaggctgac agtgatgcgc ccggcgggaa agctgaaacc
     1141 ggaattcgtg agcgcggcgc gcacgcgctc cttgctctcc ttgatgaccg tcgcaggcag
     1201 tcccacgatg cagaaggacg gaaggccact ggcgaggctc acctcgacct gcaccagcgg
     1261 agcgtgcaat ccgagctgcg cacggcaggc gaccttagct acgcccatgg cagccgaggc
     1321 tccaccgggt gccgctcaca cagcgggcgc acctccgctg ccgggagctt tgggtgccgc
     1381 gatgcccgag ctgccctcga gcgcagtgag ccgcgcctcg agtgcctcca gctgcgcgcg
     1441 ggttcgctcg agaacacgcg tctgagcatc gaactcgtcg cggctcacca gatcgagctg
     1501 gctcagccgc tcgcgcaaca ctgcacggaa attgctttcc aggtccgcct gcatgctgcg
     1561 cagcgcgggc ggcacccgct cgagtagtcg ccgggcgagc tcatcaatcc gaaatgcatc
     1621 catagcggcg agcctcgtgt gcggctgccc cgattcgggg cagcgtcgat ggcggcggct
     1681 ccaatttggt gcagcacctc aggcacaccc ggcgcaagtc gcggaaacat cgagccttac
     1741 gccatggcat ggaatctgca ccctttccgc gcgcttaacc agcttaacaa gccggccgcg
     1801 gtgcgtgcag ccgctgttca tggtgcaaac gacaggaaca gagaccatgt ccatccttgc
     1861 caccccgctt cggcagtcgg cttgcctgac cgccgcgctt gcagcgcttg ctccggggct
     1921 cgccgctgcc gccgaggcgc cgctgccgag caagggtgac accgcctgga tgctcaccgc
     1981 caccgcgctc gtgctgctga tgtcggtgcc ggcgctcgca ctcttctacg gcgggctcgt
     2041 gcgtaccaag aacatgctgt cggtactcat gcaggtattc gtcggcttct ccctcatcac
     2101 cgtgctgtgg tgcgtgtacg gttattcgct cgcctttacc gagcacaacg ccatcatcgg
     2161 cggattcaac cggctgtttc tcaacggcac cttcgatgcg aagagcggca ctctcgcgct
     2221 cgcctccacc ttcagcaagc acaccccgat gtacgagctg gtatatgtgg cgttccaggc
     2281 gaccttcgcc gccatcgaga agcttccgtc ggccggattg aacgtgccgt tgagaaatgc
     2341 gcgcgacaat ccgccgaaga atgcgttgcc ttcggtgaac gcgacgctgt agccgtacac
     2401 gcaccacagc acgctga
//
LOCUS       DATDMP010000123         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_241191, whole genome shotgun sequence.
ACCESSION   DATDMP010000123 DATDMP010000000
VERSION     DATDMP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241191"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VI195_02070"
     CDS             <1..1277
                     /locus_tag="VI195_02070"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6823199.1"
                     /translation="VETNTTHSAGSTTLAAQRMAKRLQEAGFAESETIILEPFPKKGN
                     LVVRMRGDGSKRPLLLLAHLDVVEAMRSDWKTDPFVLQETGGYYTARGSIDDKAMASS
                     FVSILGQLKREGFRPKRDIILALTTDEERGDVETNGVYWIVNHKRELVDAELGLNEGG
                     GGELRNGKPLLNRIQVAEKMYTTYRFTVRNPGGHSSLPVPDNAIYQLSEALLRVGEHR
                     FPVKLAEVTRSYFERTAKVVSGPLANDMRAVASGEADAAVYDRLSKSPNYNALLRTTC
                     VATQVQAGHAENALPQSASATVNCRILPHDDPAAVDAEVRRLAGPKVEVNAINPPLAS
                     PPSPLRPDVMSPVEEITKAMWPGIPVVPSMSTGATDSRFLRNLGMPVYGVSGLFVEPS
                     DYRAHGLDERVPVASLYASREFLYRLVKRLAE"
     gene            1288..>2434
                     /locus_tag="VI195_02075"
     CDS             1288..>2434
                     /locus_tag="VI195_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEY6823200.1"
                     /translation="MAKVYAADDPQLGRVTYRDRKRFWWSLSVVYPLLPFTGMAAHAW
                     TGWQLALGLPLVISYGIMPLLDYFIGEDDNNPPEAVVPQLEQDRYYRLLTWITVPLHF
                     VALIGCAWWVGTHDLSWWAVLMFAYVAGTDSGLGINTGHELGHKYNRVEQWLARLVLA
                     VPAYGHFTVEHGRGHHRWVSTPEDHASARMGESIYRFSLRELPGGIRRAWRLEKKRLE
                     KEGRSAWSVHNTMLQSYAVSAVLQLGLIAAFGWIMIPFLAVHNVIAWWQLTSANYVEH
                     YGLLRRRLPNGEYEAPQPHHSWNANHLVTNLALFHLQRHSDHHSYPSRRYQSLRDFPD
                     LPTLPSGYFGMFPLSYVPPLWFRVMDPRLLALPHVQGDLSRVNIDPRK"
BASE COUNT          408 a          853 c          778 g          395 t
ORIGIN      
        1 tggtcgagac caacacgacg cactcggccg gcagcaccac gctcgccgcg cagcgcatgg
       61 cgaagcgcct gcaggaagcg ggcttcgccg aatccgagac gatcatcctc gagccctttc
      121 ccaagaaggg aaacctcgtc gtgcggatgc gcggcgacgg tagcaagcgg cccttactcc
      181 tcctcgcgca cctcgacgtc gtggaagcga tgcgcagcga ctggaagacc gatccgttcg
      241 tgctgcagga gacgggcggg tactacaccg cgcgcggctc gatcgacgac aaggcgatgg
      301 cgtcctcctt cgtgtcgatt ctcggccagc tcaagcgcga agggttcagg ccgaagcgcg
      361 acatcatcct cgcgctcact accgacgagg agcgcggcga tgtggagacg aacggcgtct
      421 actggatcgt caaccacaag cgcgagctgg tcgacgccga gctcggcctc aacgaaggcg
      481 gcggcggcga gttgcgcaac ggcaagccgc tcctcaaccg catccaggtg gccgagaaga
      541 tgtacacgac gtaccggttc acggtgcgca atcccggcgg gcacagctcg ctgccggttc
      601 cggacaacgc catctaccag ctctcggaag cgctgctgcg agtaggcgag caccgctttc
      661 cggtgaagct cgccgaagtg acccgctcgt acttcgagcg caccgcgaag gtggtgagcg
      721 gcccgctggc gaacgacatg cgcgctgtgg caagcggcga agccgacgcg gcggtctacg
      781 atcggctctc gaagagcccg aactacaacg cgctccttcg caccacctgc gtcgccacgc
      841 aggtgcaggc cgggcacgcg gaaaacgcgc tgccgcagtc ggcgagcgca accgtgaact
      901 gccgcatcct tccccacgat gatcctgccg cggtggatgc cgaggtgcgt cggctcgcgg
      961 gaccgaaggt cgaggtgaac gcaatcaacc cgccgctggc gagccctccc tcgccgctgc
     1021 ggccggacgt gatgtcgccg gtcgaggaga tcaccaaggc gatgtggccc ggcatcccgg
     1081 tcgtcccgtc catgagcacc ggcgcgaccg acagccggtt cctgcgcaac ctcggcatgc
     1141 cggtgtacgg cgtctccggg ctcttcgtcg aaccctcgga ttaccgcgcg cacggcctcg
     1201 acgagcgagt gccggttgcg tcgctctacg cctcgcgcga attcctctac cggctggtca
     1261 agcgcctggc ggaatagact tacgcctatg gcgaaggtct acgccgccga cgatccgcag
     1321 ctcggacgcg tcacctatcg cgaccgcaag cgcttctggt ggtcgctgtc ggtcgtgtat
     1381 ccgctgctgc ccttcaccgg aatggcggcg catgcctgga cgggatggca gctcgcactc
     1441 ggcctgccgc ttgtcatcag ctacggcatc atgccgctgc tcgactactt catcggcgag
     1501 gacgacaaca acccgcccga ggcggtggtg ccgcagctcg agcaggatcg ctactaccgc
     1561 ctgctgacct ggatcacggt gccgctgcac ttcgtggcgc tgatcggctg cgcgtggtgg
     1621 gtcggtacgc acgacctttc gtggtgggcg gtgctgatgt tcgcctacgt cgccggcacc
     1681 gattccggcc tcggcatcaa cacggggcac gagctcgggc acaagtacaa ccgcgtcgag
     1741 cagtggctgg cgcggctcgt gctcgccgta cccgcgtacg ggcatttcac cgtcgagcac
     1801 gggcgcggcc atcatcgctg ggtctcgacg cccgaagacc acgccagcgc ccgcatgggc
     1861 gagagcatct accgcttctc gctgcgcgag ttgccgggcg gcatccgccg cgcctggcgg
     1921 ctcgagaaga agcggctgga gaaagaaggc aggagcgcat ggagcgtgca caacaccatg
     1981 ctgcagtcct acgcggtgag cgccgtgctg cagctcggcc tgatcgccgc cttcggctgg
     2041 atcatgattc ccttcctcgc cgtgcacaac gtcatcgcgt ggtggcagct gacctcggcg
     2101 aattacgtcg agcactacgg cctgctgcgc cgccgcctgc cgaacggcga gtacgaggcg
     2161 ccgcagccgc atcattcgtg gaacgccaac cacctggtga ccaacctcgc gctcttccac
     2221 ctgcagcgcc attccgatca ccattcgtac ccttcgcgcc gctaccagtc gctgcgcgac
     2281 ttcccggacc tgccgaccct gccgagcggc tacttcggca tgtttccgct gtcctacgtt
     2341 ccgccgctgt ggttccgcgt gatggatcct cgcctgctcg cgctgcctca cgtgcaaggc
     2401 gatctcagcc gcgtgaacat cgatccgcgc aagc
//
LOCUS       DATDMP010000124         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_241208, whole genome shotgun sequence.
ACCESSION   DATDMP010000124 DATDMP010000000
VERSION     DATDMP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241208"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..666)
                     /locus_tag="VI195_02080"
     CDS             complement(61..666)
                     /locus_tag="VI195_02080"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HEY6823201.1"
                     /translation="MAEILGRSAALGGALVLAVLLLAGCTDQTKVHETELSELLVLLP
                     GHYDNTAQVEADARNHTSPQHDAVALTITHVFTPRLGHHVYYAQETAADDPRRVFSQK
                     MYAFEVDEKRGIVETLFEFVEPVRWRDGQQNKDIFTVVTTDDVQPEGCQLLWKKKDSG
                     FVATHDARACPDASGTVVPQAEFNGGVLTIGEYKFRRTRAH"
     gene            complement(659..1693)
                     /locus_tag="VI195_02085"
     CDS             complement(659..1693)
                     /locus_tag="VI195_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823202.1"
                     /translation="MKRLRALVVVHASLVPPESLDGHSDKEIEEWRTEYDVTSNLRKL
                     GHEVRCLGVLDSLTELRSAIADWQPDIVFNLLEEFDGIVTYDQHVVAFLELMRQPYTG
                     CNPRGLLLSRDKSLCKQLLSFHRIATPQFAVFRRGVRVHVPRKLRFPLFVKSTVEDAS
                     LGIAQASVVEDAARLKERIAFVHEQVKSDALVEEYIEGRELYVGVMGNERLTRLPVWE
                     MVFGSMPDSLAAIATRKVKWDKRYQAKYGITTHAAVDLSPQVLAALDRLSRRIYRALG
                     MSGYARMDFRVTQQGQVYVLEANANPNLETAEDFAESARAAGTPYGELLERLMDLGLK
                     YRAQWRASYG"
     gene            complement(1690..2655)
                     /locus_tag="VI195_02090"
     CDS             complement(1690..2655)
                     /locus_tag="VI195_02090"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEY6823203.1"
                     /translation="MRFCDLRLSIERSAIAPHLRRLYSDLERRGLSLRPHAWLSEEWF
                     SPDGVPGIAVPFYLAHPRLERLERRIMGEAEGGDSRLLLRILRHEAGHALDNAYRLRR
                     RRRWREVFGPASRPYPARYRARAGSRRYVHHLGEWYAQAHPTEDFAETFAVWLAPKSG
                     WRKNYAGWPALHKLQAVDELIASVRDRRPPVRNRLRIEPLDHNRRTLAQHYRRKLARN
                     RLIRRGLADELLQRAFTTQPPRRGALRATRLLRRHARALTASVARALGSEHYSVEQVL
                     QMLIERSAELGLYVRNRRDALRYSRWMLERLTAVYSQGETPHLPL"
BASE COUNT          508 a          982 c          980 g          473 t
ORIGIN      
        1 ctgaaaaaca tacctcacca gccgagcact ccgtagaggc cggggcgaac ggcgggcgag
       61 ttaatgcgcc cgcgtacggc ggaacttgta ctcgcctatg gtgagcaccc cgccgttgaa
      121 ctcggcctgc ggcacgaccg tccctgaagc atcaggacag gcccttgcat cgtgcgtcgc
      181 gacgaagccg gagtccttct tcttccacag cagctggcac ccttccggct gcacatcgtc
      241 tgtggtcacg acggtgaata tgtccttgtt ctgctgtcca tcgcgccagc gcaccggctc
      301 gacgaactcg aagagcgtct cgacgatgcc gcgcttctcg tcgacctcga aggcatacat
      361 cttctgactg aaaacacggc ggggatcgtc cgcggccgtt tcctgcgcgt agtacacgtg
      421 atggcccagg cgcggcgtga acacatgcgt aatggtgagc gccacggcat cgtgctgcgg
      481 gctcgtgtga ttgcgcgcat cggcctccac ctgcgcggtg ttgtcgtagt ggccgggcag
      541 cagcacgagc agctcgctga gctcggtttc gtggaccttg gtctgatcgg tgcagcccgc
      601 gagcagcagc accgcgagca cgagtgcacc gccgagcgcg gcgctgcggc cgaggatctc
      661 agccatagga cgcccgccac tgcgcccggt acttcagccc caggtccatg aggcgctcga
      721 gcagctcgcc atacggcgtg ccggccgcgc gcgcagattc ggcaaaatcc tcggcagtct
      781 cgagattggg gttggcgttg gcctcgagca cgtacacctg gccctgctgg gtcacgcgga
      841 agtccatgcg agcataaccc gacataccga gcgcccggta gatgcggcgc gagaggcgat
      901 cgagcgcggc cagcacctgc ggtgacagat ccaccgcggc atgcgtggtg ataccgtact
      961 tcgcctggta gcgcttgtcc cacttcacct tgcgggtcgc aatcgccgcg agcgaatcgg
     1021 gcatcgagcc gaacaccatc tcccacaccg gtagcctggt gaggcgctcg ttgcccatga
     1081 cgccgacgta cagctcgcgg ccctcgatgt actcctccac cagcgcatcg gacttcacct
     1141 gctcgtgcac gaatgcgatg cgctctttca gtcgcgccgc gtcctccacc accgaggcct
     1201 gcgcgatgcc gagcgaggca tcctccacgg tcgacttcac gaacagcgga aagcgcagct
     1261 tgcgcggcac gtgcacacgt acgccgcggc ggaacacggc gaattgcggc gtggcgatgc
     1321 ggtgaaagct gagcagctgc ttgcacagcg acttgtcgcg cgacagcagc agcccgcgcg
     1381 gattgcagcc cgtgtagggc tgacgcatca gctcgagaaa cgccaccacg tgctggtcgt
     1441 aggtgacgat gccgtcgaat tcctccaaca gattgaacac gatgtccggt tgccagtcgg
     1501 cgatcgcgct gcgcagctcc gtgaggctgt cgagcactcc gagacagcgc acttcatggc
     1561 cgagcttgcg caaattgctg gtgacgtcgt actcggtgcg ccactcctcg atttccttgt
     1621 cgctgtggcc gtcgagactt tccgggggca ccaggctcgc gtgcaccacc acgagcgccc
     1681 gcagccgttt cataacggca aatgaggagt ctcgccctgg gagtagaccg cggtgagccg
     1741 ctcgagcatc cagcgtgaat agcgcaacgc atcgcggcga ttgcgcacgt acagcccaag
     1801 ttccgcactg cgctcgatca gcatttgcag cacctgctcg acactgtagt gctcgctgcc
     1861 gagcgcgcgg gcgacgctcg cggtaagcgc ccgcgcatgt cggcgcagca gtctcgtggc
     1921 gcgcagcgcg ccgcgccgcg gcggttgcgt cgtgaacgca cgctgcagca gctcatcggc
     1981 gagtccgcgc cgtatcagac ggttgcgcgc cagcttgcgc cgatagtgct gcgccagagt
     2041 gcggcggttg tgatcgaggg gctcgatacg caggcggtta cgcaccgggg gccgccggtc
     2101 gcgcacgctc gcgatgagct catccaccgc ctgcagcttg tgcaaagccg gccagccggc
     2161 atagttcttg cgccaacccg atttgggcgc aagccacacc gcgaaggttt cggcgaaatc
     2221 ctcggtggga tgagcctggg cgtaccattc gccgagatgg tgcacgtagc gccggctgcc
     2281 ggcgcgcgcg cgatagcgcg ccgggtatgg tcgcgaggcc ggcccgaaca cctcgcgcca
     2341 gcgccgccgc cgccgcagcc ggtatgcgtt gtcgagagcg tgcccggcct cgtgccgcag
     2401 aatgcgcagc agcagacgcg agtcaccgcc ctcggcctcg cccatgatgc gccgctcgag
     2461 tcgctcgagc cgcgggtgag cgagatagaa cggcacggcg atgccgggta cgccatcggg
     2521 ggagaaccac tcctccgaca gccacgcgtg cgggcgcagt gacaggccgc gccgctcgag
     2581 gtccgagtag agcctgcgca ggtgaggcgc aatcgccgag cgctcgatcg agagccgcag
     2641 atcgcagaag cgcatgcgca gcagctgttc atcggtgaat cgaacccagg gtcgagcgcg
     2701 gcggcgcgct ctgggggttg cgcgcgtgga ccgagcgcat gcggagcgcg cggaggatgc
     2761 gtggcgcttg acggcgcgcg gcagcgacat acgtgctggg cgaagactca gggggagaga
     2821 aaccggaagc gagcttaccc aagtgcccgc gcgctgtctg cggaagatcg cacttcgcgc
     2881 ctaccggatc gcaggaaatt tccacctcga ctcggggcca tcgccaagct ggccagcgga
     2941 ggt
//
LOCUS       DATDMP010000125         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1463130, whole genome shotgun sequence.
ACCESSION   DATDMP010000125 DATDMP010000000
VERSION     DATDMP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463130"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VI195_02095"
     CDS             <1..140
                     /locus_tag="VI195_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEY6823204.1"
                     /translation="KRYSNWPTYPQLYVKGELVGGCDIALEMYRNGELRKLLTEIGAA
                     K"
     gene            complement(174..920)
                     /gene="rnt"
                     /locus_tag="VI195_02100"
     CDS             complement(174..920)
                     /gene="rnt"
                     /locus_tag="VI195_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEY6823205.1"
                     /translation="MNDALPNSVPNADPAAAALMSRRFRGYLPVAIDVETGGFNVTTD
                     ALLEIAVVLIDMDGAGTLRRGATHDFHVQPFDGARLDPASLAVTGIDPWHPLRPALPE
                     RDALQRLFREVRHAVRAYNCRRAILVGHNAAFDLACINAAVARTDVKRNPFHPFSCFD
                     TATLAGAALGQTVLAKAITVAGLEWDADRAHSARYDAERSAELFCLVCNRLRDSHRSA
                     EERARALGWVNEAAEVAGEHPALDAELPPL"
     gene            complement(947..1978)
                     /gene="pyrC"
                     /locus_tag="VI195_02105"
     CDS             complement(947..1978)
                     /gene="pyrC"
                     /locus_tag="VI195_02105"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY6823206.1"
                     /translation="MSALVIRRPDDWHLHLRDGAALAAVLPFSAARFARAIVMPNLSP
                     PVTTTAAALAYRERIRAALPAGAQFEPLMTLYLTDRTQAEEIARAKAAGVLGCKLYPA
                     GATTHSDAGVTDVRRLDAVFERMAEAGLVLQVHGEVSDPAVDVFEREVRFIDTVLAPL
                     AARHARLRIVFEHITTRAALEFVLNAREGIAATVTPQHLLMNRNALFAGGLRPHHYCL
                     PVLKAEEHRVALLAAVARGSPRLFLGTDSAPHARSAKEAARACAGIFSAHAGIELYAE
                     AFAAAGALESLDPFASGYGADFYGLPRNRGTITLLPEPWEVPARYAFGTEELVPLRAG
                     ERIAWRLAA"
BASE COUNT          341 a          685 c          713 g          289 t
ORIGIN      
        1 tgaagcgcta ctccaactgg ccgacctatc cgcagctgta cgtcaagggc gagctcgtgg
       61 gcggctgcga cattgcgctc gagatgtacc gcaacggcga gctgcgtaag cttctcaccg
      121 agatcggcgc cgcgaagtaa gccagcggca ccgcggccag ccaaccgggg tggctagagc
      181 ggcggcaact ctgcatcgag tgcggggtgc tcgccagcaa cttccgccgc ctcgttcacc
      241 caaccgagcg cacgcgcccg ttcctcggcg ctgcggtggc tgtcacgcag gcggttgcac
      301 accaggcaga agagctcggc gctgcgttcg gcgtcgtaac gcgcactgtg cgcgcggtcc
      361 gcatcccatt cgagtccggc aacggtgatc gccttggcga gcaccgtctg gccgagcgcg
      421 gcaccggcga gcgtggcggt atcgaagcac gagaagggat gaaagggatt gcgcttcaca
      481 tccgtgcgcg ccacggcggc gttgatgcag gcgagatcga aggcggcgtt gtgcccgacg
      541 aggatcgcac ggcggcagtt gtaggcacgc accgcgtggc gcacttcgcg gaacagccgc
      601 tgcagcgcat cgcgctcggg cagagccgga cgcagcggat gccagggatc gatcccggtg
      661 accgcgagcg atgccggatc gagccgcgcg ccatcgaagg gctgcacgtg gaagtcgtgc
      721 gttgcgccac gccgcagcgt gcctgcgccg tccatgtcga tgagcaccac ggcaatctcg
      781 agcaacgcgt cggtcgtgac gttgaaaccg ccggtctcca catcgatcgc caccggcagg
      841 tagccgcgaa agcgccgtga catgagcgcc gcggcggcgg ggtccgcatt cggcactgag
      901 ttaggcagcg cgtcgttcat gcaccggctc tgaaaagaga gtcgcctcag gcggcgaggc
      961 gccaggctat gcgctctccc gcccgcaacg gcacgagctc ctcggtgccg aaagcatagc
     1021 gtgcgggcac ctcccagggc tcgggaagaa gagtgatcgt gccgcgattg cgcggcaagc
     1081 cgtagaagtc cgcaccatac ccggaagcga acggatcgag gctctccagt gcgcccgccg
     1141 ccgcgaaggc ttcggcgtac agctcgatcc cagcgtgggc cgagaagatg ccggcgcagg
     1201 cgcgggcggc ctccttggcg gagcgggcgt gcggagcgct gtcggtgccg agaaacaatc
     1261 gcgggctgcc gcgcgcgacc gccgcaagca gggcgacgcg atgctcttcc gccttcagta
     1321 ccggcaggca gtagtgatgc ggccgcaatc ctccggcgaa cagcgcgttg cggttcatga
     1381 gcaggtgctg tggcgtgacc gtggcggcga tgccctcacg tgcgttcagc acgaactcga
     1441 gggccgcgcg cgtggtgatg tgctcgaaca cgatgcgaag gcgcgcatga cgcgcagcga
     1501 gcggcgcaag caccgtatcg atgaaccgca cctcgcgctc gaacacgtcc accgcggggt
     1561 cgctcacttc gccatgtacc tgcagcacga gccccgcctc cgccatgcgc tcgaacaccg
     1621 cgtcgaggcg gcgcacatcg gtcactccgg catccgagtg cgtggtagcg cccgccgggt
     1681 agagcttgca gccgagcacg cccgccgctt tggcgcgtgc gatctcctcc gcttgcgtcc
     1741 gatcggtgag atacagcgtc atcagcggct cgaactgcgc tccggcggga agcgcggccc
     1801 ggatgcgctc gcgataggca agcgccgctg ccgtcgtggt caccggcggg ctcaggttcg
     1861 gcatgacgat ggcgcgcgcg aagcgcgccg cgctgaaggg cagcaccgcg gcgagcgccg
     1921 caccatcgcg caggtgcagg tgccagtcat ccggccggcg gatgacgagc gcgctcaagc
     1981 tgcgccctcc agctgctgcg tcaccttctg cttcagcagc agctcgag
//
LOCUS       DATDMP010000126         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_574783, whole genome shotgun sequence.
ACCESSION   DATDMP010000126 DATDMP010000000
VERSION     DATDMP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574783"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VI195_02110"
     CDS             <1..185
                     /locus_tag="VI195_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY6823207.1"
                     /translation="RQLRAALDAEVGRRGCEVYYPEPELCTDNGAMIAFAAAAHLQRS
                     EAATPRAFTVKPRLPL"
     gene            complement(182..781)
                     /gene="plsY"
                     /locus_tag="VI195_02115"
     CDS             complement(182..781)
                     /gene="plsY"
                     /locus_tag="VI195_02115"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY6823208.1"
                     /translation="METVLPILITILVAYLIGSISFAILVTRAMQLPDPRSYGSKNPG
                     ATNVLRSGSKLAAVLTLLGDAAKGLIAVWLAQRSVPEATPYAALAVFLGHLFPVYHRF
                     TGGKGVATAAGVLLAIDWRLGVGTLATWLIIVAFVRYSSLAAMIAAAFAPFFTALLMG
                     FDAYFACVLVMAVLLVWRHRGNIARLMAGSESRIGSKKA"
     gene            810..1166
                     /locus_tag="VI195_02120"
     CDS             810..1166
                     /locus_tag="VI195_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY6823209.1"
                     /translation="MDAILIRDLRVEAVIGIHRRERHVPQTLSIDLDIGLPGAEVFAS
                     DRVADTIDYEQVALRIRALAGAQHYRLVETFANQVAGILVSEFGAPWVKVSVAKIGIL
                     PNAKFVGITIERRRPA"
     gene            complement(1138..1605)
                     /gene="ybaK"
                     /locus_tag="VI195_02125"
     CDS             complement(1138..1605)
                     /gene="ybaK"
                     /locus_tag="VI195_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY6823210.1"
                     /translation="MNTPATQMLSKHGVPYTVHEYDYVEHGGTSVSSESLGVPEHEVV
                     KTLVMEDEEKKPLIVLMHGDRKVSTKDLARQAARKKIEPCRPEVAQRHTGYQVGGTSP
                     FGLRKPMPVFIERSILAMPAIYINGGRRGFLVRITPADLVRLLSPRPVDVARS"
     gene            complement(1602..>2242)
                     /locus_tag="VI195_02130"
     CDS             complement(1602..>2242)
                     /locus_tag="VI195_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY6823211.1"
                     /translation="CVRERLIEADPTLKLDPPKRSPRFPKTLSEHDVEALLAAPQAAT
                     PMGLRDRAMLEMLYATGLRVSELVGVKLHALNLQAGVVRVLGKGSKERLVPLGEEAVD
                     WIQRYLDEARPALRGKREPDTLFITARGAGMTRQAFWHLIRRYGMRAIPGKRLSPHVL
                     RHAFATHLINHGADLRVVQMLLGHADISTTQIYTHVARERLKQLHAKHHPRG"
BASE COUNT          401 a          741 c          741 g          359 t
ORIGIN      
        1 atcggcagct ccgcgcggcg ctcgatgccg aggtggggcg gcgcggttgc gaggtgtact
       61 acccggagcc ggagctctgc accgacaacg gcgcgatgat cgccttcgcc gcggcggcgc
      121 acctgcaaag gagcgaagcg gctacgcctc gcgccttcac ggtcaagccg cgcctcccgc
      181 tctaggcttt cttgctgccg atgcgcgact cgctgcccgc cataaggcgt gcgatgttgc
      241 ctcgatggcg ccagacgagc agtacggcca tcaccagcac gcaggcgaaa taagcatcga
      301 accccatgag cagcgccgta aagaacggcg cgaatgccgc ggcgatcatc gccgcgaggg
      361 atgaatagcg cacgaacgca acgatgatca gccaggtagc gagcgtgccg acacccagcc
      421 gccagtcgat ggcgagcagc acgccggctg cggtcgcgac gcctttgccg ccggtgaagc
      481 ggtggtagac gggaaagaga tggccgagga ataccgcgag cgccgcatac ggcgtggctt
      541 ccggtacgga gcgctgcgcc agccagacgg cgatcaatcc cttggcagca tcgccgagca
      601 gcgtgagcac cgcggccaac ttgctgcccg agcgaaggac attggtcgcg ccgggattct
      661 tggagccgta gctgcgcggg tcgggcagct gcatcgcccg ggtgacaagg atggcgaagg
      721 agatggaacc gatgaggtaa gccaccagta tcgttatcag tataggcagc accgtctcca
      781 tcgcatcgat tctatagaat cggcgcgcga tggatgccat cctcatccgg gacctgcgcg
      841 tggaagcggt gatcggcatc caccggcgcg agcgccacgt gccgcagacc ttgtcgatcg
      901 acctcgacat cggcctgccg ggcgccgagg tgttcgcgag cgaccgcgtc gccgacacca
      961 tcgactacga gcaggtggcc cttcgcatcc gcgcgctcgc cggcgcgcag cactaccgcc
     1021 tggtcgagac cttcgccaac caagtagccg gtattctggt cagcgagttc ggtgcgccct
     1081 gggtgaaggt gagcgtcgcg aagatcggca tcctgccgaa cgccaagttc gtcggcatca
     1141 cgatcgagcg acgtcgaccg gcctaggcga tagcagccgc acgagatcgg cgggcgtgat
     1201 gcgcacgaga aaaccgcgcc gcccgccatt gatgtagatc gccggcatcg cgaggatcga
     1261 gcgctcgatg aacaccggca tcggctttcg cagtccgaaa ggcgaggtgc caccgacctg
     1321 gtagccggtg tgccgctgcg ccacctccgg ccggcacggc tcgattttct tgcgcgcggc
     1381 ctggcgcgcg agatccttgg tcgagacctt gcgatcgccg tgcatcagca caatcagcgg
     1441 cttcttttcc tcatcctcca tgaccagcgt cttcaccacc tcgtgctccg gcacgccgag
     1501 cgattcggag gacaccgacg tgccgccgtg ctcgacgtag tcgtactcgt gcaccgtgta
     1561 cggcacgcca tgcttcgaaa gcatctgcgt cgccggcgta ttcatccgcg cggatggtgc
     1621 ttcgcgtgca gctgcttcag gcgctcgcgc gcgacgtgcg tgtagatctg ggtggtcgaa
     1681 atatcggcgt gcccgaggag catctgcacc acgcgcaaat ccgcgccatg attaatgagg
     1741 tgcgtggcaa aggcgtggcg cagaacgtgc ggcgagaggc gcttgccggg aatggcgcgc
     1801 atgccgtagc ggcggatgag atgccagaac gcctgccgtg tcatgccggc gccgcgcgcc
     1861 gtaatgaaaa gcgtgtcggg ctcgcgcttg cctcggagcg ccggccgcgc ttcgtcgaga
     1921 tagcgctgga tccagtccac cgcttcttca ccgagcggca cgagccgctc cttcgaccct
     1981 ttaccgagca cacgcaccac acccgcctgg aggttgagcg catgcagctt gactcccacc
     2041 agctccgaga cacggagtcc ggtggcatag agcatctcca gcatcgcgcg atcccgcaat
     2101 cccatcggtg tcgccgcttg cggcgcggcg agcagcgcct cgacgtcgtg ctccgacagg
     2161 gtcttgggaa accgcggcga gcgcttgggc ggatcgagct tgagcgtcgg atcggcctcg
     2221 atcagccgct cacgcacgca gt
//
LOCUS       DATDMP010000127         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1908026, whole genome shotgun sequence.
ACCESSION   DATDMP010000127 DATDMP010000000
VERSION     DATDMP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908026"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VI195_02135"
     CDS             <1..906
                     /locus_tag="VI195_02135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY6823212.1"
                     /translation="LYRYLGQLTGEQREPVMPVPMMNIVNGGAHANNSLDIQEFMILP
                     VGAPSFREALRYGAEVFHALRQLLAARGLSIAVGDEGGFAPSLESNEAALTLILQAIE
                     RAGFRAGQDVYLGLDVASSEFFRNGRYELAGEQRSFSAAAFSDYLADLAARYPIISIE
                     DGMSEADWDGWSQLTQALGRTVQLVGDDVFVTNTKILREAVAKHIANAILIKPNQIGT
                     LTETLECIDMADKAHYAAVVSHRSGETEDSTNADIAVATAATQIKTGSLSRSDRIAKY
                     NQLLRIEAELGSVRYAGREAFPVRI"
     gene            complement(921..1610)
                     /locus_tag="VI195_02140"
     CDS             complement(921..1610)
                     /locus_tag="VI195_02140"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY6823213.1"
                     /translation="MNYEQAEVLDRLAAEYALGTLRGRARRRFERLCSQSAAARRALH
                     RWEDRWTQLSSRLPPVQPAARVWRNVSRQLFGESFAAPRAARRRGWRLAAAAGLVAVA
                     LIVGLIVRELAPPPLLAFAVLGTDSTHPVWRLERRTPLTALTIEVVGAVPPATGKSYE
                     LWALPRGGAPVSLGLLPAGGHAERKLSEPQRAALLAADKVAVSVEPLGGSPTGSPTGP
                     IVIVTNVAASG"
     gene            complement(1607..>2188)
                     /locus_tag="VI195_02145"
     CDS             complement(1607..>2188)
                     /locus_tag="VI195_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6823214.1"
                     /translation="RVGEGQPTRPVAEPDGAELEALLARCAAGDRAGLEALYARVAPL
                     LLAVLLRMLKRRDAAEDALQDVFVSVWQRARQFDPIRGRPLAWLVSMARYRAIDLQRA
                     ARPAVALTELTGLESQLPSEEALDAGEMLGTGALLRRCLEQIAAPQRRCLLLAYQEGL
                     THSEIARAVNEPLGTVKSWVRRSLLALRRCLES"
BASE COUNT          364 a          791 c          730 g          303 t
ORIGIN      
        1 ctctaccgtt acctcggaca gctcacaggc gagcagcgcg agccggtgat gcccgtcccg
       61 atgatgaaca tcgtcaacgg cggtgcgcac gccaacaaca gcctcgacat ccaggagttc
      121 atgatcctgc cggtcggcgc accgagcttc cgcgaggcgc tgcgctacgg cgcggaggtg
      181 ttccacgcgc tgcggcaact gctcgccgca cgcgggctct cgattgcggt gggcgatgag
      241 ggtggtttcg cgccgagtct cgagtccaac gaggcggcgc tcactctcat cctgcaggcc
      301 atcgagcgag ccggcttccg cgccgggcag gacgtgtacc tcggtctcga cgtggcgagc
      361 tccgaattct tccgtaacgg ccgctacgag ctcgccggcg agcagcgcag cttctccgct
      421 gcagcgttct ccgattatct ggccgatctc gccgctcgct atccgatcat cagcatcgag
      481 gacggcatga gtgaggcgga ctgggacggc tggtcgcaac tcacgcaggc tctcggcagg
      541 accgtgcagc tcgtgggcga tgacgtgttc gtcaccaata ccaagatcct gcgggaagcg
      601 gtggccaaac acatcgccaa cgcgatcctc atcaagccga accagatcgg aacgctcacc
      661 gagacgctcg agtgcatcga tatggccgac aaggcgcact acgcggcggt ggtctcgcac
      721 cgctcgggcg agaccgagga cagcaccaac gccgacattg cagtggccac cgccgccacg
      781 cagatcaaga ccggctcgct gtcgcgctcg gatcgcatcg cgaagtacaa ccagctgctg
      841 cgcatcgagg ccgagctcgg ctcggtgcgt tacgcggggc gcgaggcgtt tccggtgagg
      901 atctaggccg cggcaaccac tcagccagac gctgccacgt tggtcacgat cacgatcgga
      961 ccggtcgggc tgccggtggg tgagccgccc aagggctcga cgctgaccgc caccttgtcg
     1021 gcggcaagca gcgcggctcg ctgcggctcg ctgagcttgc gttccgcgtg accgccggca
     1081 ggcaacaggc cgagtgaaac cggcgctccg ccgcgcggca gcgcccacag ctcataggac
     1141 ttgccggtgg caggcggcac ggctccaacg acctcgatgg tgagcgcggt gagaggtgta
     1201 cgccgctcga gccgccacac cgggtgcgtg ctgtcggtgc cgaggaccgc gaatgcgagc
     1261 agcggcggcg gcgccagctc gcgcacgatg agcccgacga tgagcgccac cgctaccagc
     1321 ccggccgcgg cagcgagccg ccacccgcgc cggcgcgccg cgcgcggggc agcaaagctc
     1381 tcgccaaaga gctgccggct cacattcctc cacaccctcg ccgcgggttg caccgggggc
     1441 agtctgctac tcagctgagt ccagcgatcc tcccagcggt ggagcgcgcg gcgcgccgct
     1501 gcgctctgcg agcagagacg ctcgaagcgc cggcgggcac ggccgcgcag cgtgccgagc
     1561 gcgtactcgg cggccagacg atccagcacc tcagcttgct cgtaattcat gactcgagac
     1621 accgtcgcag cgcgagcagg ctgcgcctca cccagctctt caccgtgcca agcggctcat
     1681 tcaccgcgcg cgcaatctcg ctgtgcgtca gcccctcctg gtaggcgagc agcagacagc
     1741 gccgctgggg agcggcgatc tgctcgaggc agcgccgcag caaggcgccg gtgcccagca
     1801 tctcaccggc atcgagcgcc tcttccgacg gcaactgcga ctcgagcccg gtcagctcgg
     1861 tgagcgccac ggcggggcgc gccgcgcgct gcagatcgat cgcccggtaa cgcgccatgg
     1921 acacgagcca cgccagtgga cggccgcgga tcgggtcgaa ctggcgggca cgctgccaca
     1981 cgctcacgaa cacgtcctgc agcgcatcct cggcggcatc gcggcgcttg agcatacgca
     2041 acaacaccgc cagcagcagc ggcgctacgc gcgcatagag tgcttcgagc cccgcgcggt
     2101 cgcccgcggc gcagcgggcg agcagcgctt ccagctccgc gccgtccggc tccgccaccg
     2161 gccgagtcgg ttgtccctct cccacgcg
//
LOCUS       DATDMP010000128         2896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_464163, whole genome shotgun sequence.
ACCESSION   DATDMP010000128 DATDMP010000000
VERSION     DATDMP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464163"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VI195_02150"
     CDS             complement(<1..738)
                     /locus_tag="VI195_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY6823215.1"
                     /translation="MAELRVTLVQQPLAWQDAGANRERLAALLAPAAGGTDLVVLPET
                     FTTGFSMEVETLAEPAGGPTAAWLAALAGSLDAAITGSVITVEAGRYYNRLLWAEPRA
                     ALRHYDKRHLFRLGDEHRHFTPGGAAWSVPWRGFAVCPLVCYDLRFPVFSRRRAALDY
                     ELLVYVANWPAARAYAWRQLLRARAIENQAYVVGVNRVGPDGLGVAHAGDSAAIDYLG
                     QPLLEAGADPALLTVTLDGAKLRAFRDR"
     gene            complement(743..1906)
                     /locus_tag="VI195_02155"
     CDS             complement(743..1906)
                     /locus_tag="VI195_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEY6823216.1"
                     /translation="MRASKLPEVGTTIFTVMSRRARELGALNLGQGFPDYDIDPRRTA
                     RVGAALAAGHNQYAPMEGLPELRARIAAKLGACYGRSPDPDTEITVTLGATEAIYSAI
                     QAAVGAGDEVIAFDPAYDSYQPAVKLAGARCVRLPLAQPGFRYDWERVRAALSPRTRL
                     VLFNSPLNPACTAATAADLDALAAAIRDRDILVLSDEVYEHVVFDGVRHHSVLAHAEL
                     AARSFAVFSFGKTLHATGLRVGYCVAPPPLTRELRKVHQFNTFSIATPLQHAIAAYLT
                     EQPGCWRSLAPFFQAKRDRLRAALARSGFRLPPAQGTYFQLLDFGDFAPPDDCAFAER
                     LLTEAGVATIPLSPFYAAAEPLTFLRLCIAKRDATLDEAAARLAAFAARLGRS"
     gene            complement(1937..2686)
                     /locus_tag="VI195_02160"
     CDS             complement(1937..2686)
                     /locus_tag="VI195_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY6823217.1"
                     /translation="MTRRILGIDVGGSSIKAGLLELEAGRLSGGLISAPTPHPATPAA
                     LMPVIAALAGRLPGGSGGVGIAFPSVIKRGTARTAANVDHAWLGTDGAALATRALGRP
                     ALLLNDADAAGIAEMRWGAGRGCRGTAIMVTLGTGIGTALFSDGKLFPNSELGHLELH
                     GMDAEKWASAQVRTSEKLDFPAWIARVNEYLARLHQLLWPDLFILGGAVSERFAEFAP
                     LLRSEAQLQPAHFAGQAGVIGAALAAAEADL"
     gene            complement(2683..>2896)
                     /locus_tag="VI195_02165"
     CDS             complement(2683..>2896)
                     /locus_tag="VI195_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823218.1"
                     /translation="VAGPGSSTLPMVIFSKVRFGVTPDVNALATIMVLIVATGIALAS
                     LWMRYQQRRRERDVQLAAAADVSMPG"
BASE COUNT          417 a         1030 c         1083 g          366 t
ORIGIN      
        1 cctgtcgcgg aaggcgcgca gcttcgcgcc gtccagcgtc accgtgagca gtgcggggtc
       61 ggcgcccgcc tcgaggagcg gctgtccgag gtagtcgatc gcggcgctgt cgcccgcatg
      121 cgcgacaccg agcccgtcgg ggccgacgcg gttgacgccc accacgtaag cctggttctc
      181 gatggcgcgc gcccgcagca gctgtcgcca ggcgtaggcg cgcgccgcgg gccagttggc
      241 cacgtacacc agcagctcgt agtcgagcgc ggcgcggcgg cggctgaaga ccgggaagcg
      301 caagtcgtaa cacaccagcg ggcagacggc aaaaccgcgc cacggcacgc tccacgccgc
      361 cccgccgggg gtgaagtggc ggtgctcgtc ccctaagcgg aacaggtgcc gcttgtcgta
      421 gtgccgcagc gccgcgcgcg gctccgccca cagcagccgg ttgtagtagc ggcccgcctc
      481 gaccgtgatg acgctgccgg tgatcgcggc gtcgagcgac ccggccagcg ccgcgagcca
      541 tgccgcggtc gggccgccgg ccggctccgc cagagtctcg acctccatgc tgaacccggt
      601 ggtgaaggtc tccggcagca ccaccagatc ggtgccgccg gcggcgggtg cgagcagcgc
      661 cgccaggcgc tcgcggttgg cgcccgcgtc ctgccaggcg agcggctgct gcaccagcgt
      721 gacgcgcagc tccgccatgc gctcagctcc gccccagccg cgcggcgaaa gccgccagcc
      781 gcgccgccgc ctcgtcgagc gtcgcatcgc gcttggcgat gcacagccgc agaaaggtga
      841 gcggctcggc ggccgcgtag aacggcgaca gcggaatggt ggcgacgccc gcttcggtga
      901 gcagccgctc ggcgaaggcg caatcgtcgg ggggcgcgaa gtcgccgaag tcgagcagct
      961 ggaagtaggt gccctgcgcc ggcggcaggc ggaagcccga gcgcgcgagc gcggcgcgca
     1021 gccggtcacg cttggcctgg aaaaacggcg cgagcgagcg ccagcacccg ggctgctcgg
     1081 tgaggtaggc ggcgatcgca tgctgcagcg gagtggcgat gctgaaggtg ttgaactggt
     1141 gcaccttgcg cagctcgcgg gtgagcggcg gcggtgccac gcaatagccg acccgcagcc
     1201 cggtcgcgtg cagcgtcttg ccgaaggaga acaccgcgaa gctgcgcgcg gcgagctcgg
     1261 catgggcgag caccgagtgg tggcggacgc catcgaacac cacgtgctcg tagacttcgt
     1321 cggacagcac caggatgtcg cggtcgcgga tcgctgccgc gagcgcatcc agatcggccg
     1381 ccgtggccgc ggtgcaggcc ggattcagcg ggctgttgaa cagcaccaga cgcgtgcgtg
     1441 ggctcagcgc ggcgcgtacc cgctcccagt cgtagcggaa cccgggctgg gcgagcggca
     1501 gccgcacgca gcgcgcgccg gcgagcttga ccgccggctg gtaggaatcg taggcgggat
     1561 cgaatgcgat cacctcgtcg ccggcgccga ctgcggcctg gatggccgag tagatcgcct
     1621 cggtagcccc gagggtcacg gtgatctcgg tgtccgggtc gggactgcgg ccgtaacacg
     1681 cgccgagctt ggcggcgatg cgcgcgcgca gctccggcag cccctccatc ggtgcgtact
     1741 ggttgtggcc ggccgccagg gccgcgccca cccgcgcggt gcgccgcggg tcgatgtcgt
     1801 agtcggggaa accctggccg aggttcagcg cgccgagctc gcgcgcgcgg cgggacatga
     1861 ccgtgaagat cgtcgtgccg acctcgggaa gcttgctcgc tctcacgctc aggtccgcgc
     1921 ctcgccgcgc ctcccgctac agatcggcct cggcggcggc gagcgccgcg ccgatcacgc
     1981 cggcctgccc ggcaaagtgc gccggctgca gctgcgcctc cgagcgcagc agcggtgcga
     2041 actcggcgaa gcgctcgctc accgcgccgc cgagaatgaa cagatccggc cagagcagct
     2101 ggtgcagccg cgcgaggtac tcgttcaccc gggcaatcca cgccgggaag tcgagcttct
     2161 cgctggtccg cacctgtgcc gaggcccact tctccgcgtc catgccgtgc agctccaggt
     2221 gcccgagctc gctgttcggg aacagcttgc cgtcgctgaa cagcgcggtg ccgatcccgg
     2281 tgccgagcgt caccatgatc gccgtgccgc ggcagccccg gccggctccc cagcgcatct
     2341 cggcaatgcc ggcggcatcg gcgtcgttga ggagcagcgc cggccggccg agggcgcgcg
     2401 tggcgagcgc cgcgccgtcg gtgccgagcc aggcatgatc gacgttggcg gcggtgcgcg
     2461 ccgtgccgcg cttgatgacg ctcgggaagg cgatgccgac gccgccgctg ccgcccggaa
     2521 ggcggccggc gagcgccgcg atcaccggca tcagcgccgc gggcgtcgcg ggatgcggag
     2581 tcggggccga gatcagcccg ccggagaggc ggccggcctc gagctcgagc agcccggcct
     2641 tgatggagga tccgcccacg tcgatgccga ggatccggcg tgtcatccgg gcatgctcac
     2701 atcggcggcg gccgcgagct gcacgtcgcg ctcgcgccgg cgctgctggt agcgcatcca
     2761 cagcgacgcg agcgcgatcc cggtcgccac gatcaatacc atgatggtgg cgagcgcgtt
     2821 cacgtccggg gtcacaccga agcgcacttt ggagaagatc accatcggca gcgtgctcga
     2881 gcccggtccg gcgacg
//
LOCUS       DATDMP010000129         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1353408, whole genome shotgun sequence.
ACCESSION   DATDMP010000129 DATDMP010000000
VERSION     DATDMP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353408"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1289)
                     /locus_tag="VI195_02170"
     CDS             complement(81..1289)
                     /locus_tag="VI195_02170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6823219.1"
                     /translation="MEAKQVAEARAGAGAATTQDLPTRAKTVAAVAATHAGAVDSAAR
                     FPAEAFQAVKAQRLLGIQVPKALGGEGLGIGDVADVCYQLGQACGSTGMIFAMHQIKV
                     ACIMRHMGENPTLQRMLRRLSAEQLLLASSTTEGQGGGNIRSSEAPVEHQEGGRIALE
                     RRASVISYGAYADGVVTTARRAADAAASDQVLIAFFKSDYTLTRLQGWDALGMRGTCS
                     EGYTLKAAASAEQILPEPYETIHTRTMVPFAHLLWGSVWTGIAASATARAQGFVRNAM
                     RHSGQLPPGAPQFTRALATLRTLRGMLATSLRRYEQCMNDPPALSALEFQTLITLTKV
                     EASELAAQTVMSAMRACGLSGYRNDSEYSIGRHLRDVLSSPIMINNDRILANLATPVL
                     MSPMAATLRD"
     gene            1502..>2123
                     /locus_tag="VI195_02175"
     CDS             1502..>2123
                     /locus_tag="VI195_02175"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF016120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEY6823220.1"
                     /translation="MPDSALHATENQFFGLLREASGGQAVHLRLTSLPELPRSSEALE
                     HIRRNYWPLEEVLAGTPEALIVTGTEPRAPVLTEEPYWGRFVELLSFARAHTLASVWS
                     CLAAHAAVLSLDGIERERLAQKRCGVFEHHILSEHPLLAGVEAPMPMPHSRWNELSAH
                     SLRSAGYTLLSWSRETGADAFVREEQSLLLFFQGHPEYEDTTLLKEY"
BASE COUNT          346 a          686 c          711 g          380 t
ORIGIN      
        1 aacggtcatg gtcgcttggc tccggcacgc gagttgcgtg cgatggtcat tccttgacgc
       61 tctcaggaag cgcgcagcat tcagtcgcgc agcgtggcgg ccatcgggct catcagcacc
      121 ggcgtcgcca ggttcgcgag aatgcggtcg ttgttgatca tgatggggga ggagagcaca
      181 tcccgcagat gacggccgat gctgtactcg ctgtcgttgc ggtaaccgga gagcccgcag
      241 gcacgcatgg cgctcatgac ggtctgcgcg gcgagctccg acgcctcgac cttggtgagc
      301 gtgatcagag tctgaaactc gagggcagag agcgctggcg ggtcgttcat gcattgctcg
      361 tagcgccgca aggatgtggc gagcatcccg cgcagcgtcc gcaaggtggc gagcgcgcgc
      421 gtgaattgcg gtgcacccgg tggcagctgg ccgctgtgac gcatcgcgtt gcgcacgaag
      481 ccctgcgccc gcgcagtcgc actggccgca attccggtcc acaccgagcc ccacagcagg
      541 tgcgcgaagg gcaccatggt gcgcgtatga atggtctcgt acggctcggg caggatctgc
      601 tccgcgctcg ccgcggcctt gagcgtgtag ccttcgctgc aggtgccgcg catgccgagt
      661 gcgtcccagc cctgcaggcg cgtgagtgtg taatcgctct tgaagaaggc gatcagcacc
      721 tggtcggagg ccgcggcatc cgcggcgcgc cgcgcggtgg tgacgacgcc atcggcgtag
      781 gcgccataag agatcacact ggcgcggcgc tcgagggcga tacggccacc ctcctggtgc
      841 tcgaccggtg cctcgctcga gcggatgttg cccccgccct gcccctcggt cgtcgacgag
      901 gcgagcagca gctgctcggc gctgagccgc cgcagcatcc gctgcagcgt cgggttttcg
      961 cccatgtgcc gcatgatgca cgccaccttg atctggtgca tcgcgaagat catgccggtg
     1021 gagccgcagg cttgacccag ttggtagcac acatcggcca catcgccgat gccgagcccc
     1081 tcgcctccca gcgctttcgg cacctggatg ccgagcaggc gctgcgcctt cacagcctgg
     1141 aaggcttcgg ccgggaatcg cgcggcacta tcgaccgcgc ccgcatgagt agctgcgacg
     1201 gcggcgacag tcttggctcg agtgggcaga tcttgggtgg tggcggcgcc ggctccggcg
     1261 cgggcctctg caacctgctt agcttccatc aaaagtccct tggattagtt ttccttgtgg
     1321 gcggacggta atctagaaaa cccataatac atcagacctt gtgcgtgcct ggcggggagc
     1381 gtcacaaaag ccgctgccca ataagtgaaa tgattttatg cccgtagaga tcgagccgcg
     1441 tgctgcggta tccgctgccc gacctgccgg aagccccatc gtaatcgggc tagtgaacaa
     1501 catgcccgat tcggcgctgc acgcgacgga gaaccagttc ttcggactgc tgcgggaggc
     1561 ttccggcggc caggcggtgc acctgcggct cacctcgctc cccgagctgc cgcgatcgtc
     1621 cgaggcgctg gagcacatcc gcagaaacta ctggccgctc gaggaggtcc tggccggcac
     1681 gccggaggcg ctgatcgtga ccggaaccga gccacgcgcc ccggtgctga ccgaggagcc
     1741 ctactggggt cggttcgtgg agctgctcag cttcgcccgg gcgcataccc tcgccagtgt
     1801 ctggtcgtgc ctcgccgccc acgccgccgt cctgagcctc gatgggatcg agcgtgagcg
     1861 gctcgcgcag aagcgttgcg gtgtcttcga gcatcacatt ctgagcgagc atccgctgct
     1921 cgccggagtc gaagcaccca tgccgatgcc gcattcgcgc tggaatgagc tttcggcgca
     1981 ctcgctccgg tccgcgggtt ataccctgtt aagctggtcg agagagacgg gtgcggatgc
     2041 cttcgtgcgc gaggaacaga gtcttctgct tttcttccag ggccacccgg agtacgaaga
     2101 cacgacgctt ctgaaggaat acc
//
LOCUS       DATDMP010000130         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2131402, whole genome shotgun sequence.
ACCESSION   DATDMP010000130 DATDMP010000000
VERSION     DATDMP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131402"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VI195_02180"
     CDS             complement(<1..527)
                     /locus_tag="VI195_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823221.1"
                     /translation="MKPAQEMKGRADLETTGPNPALLSDAVTTAVSSRHPTLNTKPLF
                     KLMVEKKASDLFFTSNAPIKIKIEGQILPVNKQVLSPETVRQTAFAIMSPEQREHFVN
                     EWELDFAVSEPGLGRFRVNVFMQRGYPAMVMRYITADMPRLDSLGLPEVVTELTLMKR
                     GLLLMVGATGSGKSTT"
     gene            complement(524..1678)
                     /locus_tag="VI195_02185"
     CDS             complement(524..1678)
                     /locus_tag="VI195_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY6823222.1"
                     /translation="MDRDQAIKLMQDLLRRVTEKKASDLFITAGFPPAIKVDGEIRPQ
                     SERALTAEQSATLVRAIMNDRQTKEFDATKECNFAIAPPGIGRFRVSAFVQQAAIGCV
                     IRLINAKIPTFEELELPPILKEVVLSKRGLVIIVGGTGSGKSTTLAAMVGYRNEKTRG
                     HIVTIEDPVEYVHTHKGCVITHREVGVDTDSWAAALKNTLRQAPDVILIGEIRDRDTM
                     EYGIQFSETGHLVLATLHANSANQALDRIINFFPDERRDQLLMDLSLNIRALISQRLI
                     PRESGAGRIAAMEIMLASPLIQDLIFKGEVAKIKEVMSRSNRLGMKTFDQALFELYET
                     GFISYEDALRNADSKNELRLRIKLDSKREQKNIDDGGALKIVEEEQGRSL"
     gene            complement(1730..2770)
                     /locus_tag="VI195_02190"
     CDS             complement(1730..2770)
                     /locus_tag="VI195_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY6823223.1"
                     /translation="MAVDIAQLLAFAVKNNASDLHLSSGVPPMIRVDGDMKRINMPAL
                     SHKEVHSMVYDIMNDKQRKAYEEFFETDFSFEIPKLARFRVNAFNQNRGAGAVFRNIP
                     STILSLDDLNAPKIFRDLCMLPRGLVLVTGPTGSGKSTTLAGMVNHCNDNRPDHIITI
                     EDPIEFVHESKRCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALT
                     AAETGHLVFGTLHTSSAAKTIDRIVDVFPGEEKDMVRSMLSESLRAVISQTLLKKNGG
                     GRVAAHEIMIGTPAIRNLIREGKIAQMYSSIQTGGAQGMQTLDQCLTDLVAKGQVSKE
                     EARYKAQNKDQL"
BASE COUNT          498 a          950 c         1008 g          595 t
ORIGIN      
        1 gtggttgact tgcccgagcc ggtggcgccc accatcagca gcagcccgcg cttcatgagc
       61 gtcagctcgg tcaccacctc cggcaggccc agcgagtcga gccgcggcat gtcggcggtg
      121 atgtagcgca tcaccatggc cggatagccg cgctgcatga acacgttgac gcggaagcgc
      181 ccgagcccgg gctcggagac cgcgaagtcg agctcccact cgttgacgaa atgctcgcgc
      241 tgctcgggtg acatgatcgc gaacgcggtc tggcgcaccg tctcggggct gagcacctgc
      301 ttgtttaccg gcaggatctg cccctcgatc ttgatcttga tgggcgcatt ggaggtgaag
      361 aacaggtccg aggccttctt ctccaccatc agcttgaaca gcggcttggt gttgagagtc
      421 gggtgacgcg agctcaccgc cgtggtgacc gcgtcgctga gcagcgccgg gttcggaccg
      481 gtagtctcga gatcggcgcg ccccttcatc tcttgtgctg gtttcacaga ctgcgaccct
      541 gttcttcctc gactatcttg agtgcgccgc cgtcgtcgat gttcttctgc tcacgcttgc
      601 tgtcgagctt gatgcgcaac cgcagctcgt tcttcgagtc ggcgttgcgc agcgcatcct
      661 cgtaggagat gaagccggtc tcgtacagct cgaacaatgc ctggtcgaag gtcttcatcc
      721 cgagccggtt ggagcgcgac atgacctcct tgatcttggc cacctcgccc ttgaagatca
      781 gatcctggat caggggcgat gccagcatga tctccatcgc cgcgatgcgt ccggcgccgg
      841 actcgcgtgg tatgagccgc tgcgagatga gcgcgcggat attgagcgac aggtccatga
      901 gcagctgatc gcgccgctcg tccgggaaga agttgatgat gcggtcgagc gcctggttgg
      961 cgctgttggc gtgcagcgtg gcgagcacca ggtgaccggt ctcggaaaac tggatgccgt
     1021 actccatggt gtcgcggtcg cggatctcac cgatcaggat cacatccggc gcctggcgca
     1081 gcgtgttctt gagcgcagcg gcccaggaat cggtatccac gccgacctcg cgatgggtga
     1141 tgacacagcc cttgtgggtg tgcacgtact cgaccggatc ctcgatggtg acgatgtgtc
     1201 cgcgggtctt ctcgttgcgg tagccgacca tggcggcgag cgtggtggat ttacccgagc
     1261 cggtgccgcc gacgatgatc accagtccgc gcttcgacag caccacttcc ttgaggatcg
     1321 gcggcagctc gagctcctcg aaggtcggaa tcttggcgtt gatcaggcgg atcacgcagc
     1381 cgatcgccgc ctgctgcacg aaggcgctga cacggaagcg gccgatgccg ggcggcgcga
     1441 tggcgaaatt acactccttg gtggcgtcga actccttggt ctgacggtcg ttcatgatgg
     1501 cgcgcaccag tgtcgccgac tgctcggcgg tgagcgcacg ctcggactgc gggcggatct
     1561 cgccgtcgac cttgatggcc ggcgggaagc ccgcggtgat gaacaggtcc gacgccttct
     1621 tctcggtgac ccgccgcagc aggtcctgca tcagcttgat ggcctggtca cggtccatgc
     1681 tgccgcccct gcgtcgctgc caccggcgcc ttcactcccg cgctgtgcat cacaactgat
     1741 ccttgttctg cgccttgtag cgcgcctctt ccttgctcac ctggcccttg gccaccaggt
     1801 ccgtgaggca ctgatcgagg gtctgcatgc cctgcgcgcc gccggtctgg atcgaggagt
     1861 acatctgcgc gatcttgccc tcgcggatga ggttccggat cgccggggtg ccgatcatga
     1921 tctcgtgcgc cgccacgcgc ccaccgccgt ttttcttcag cagcgtctgc gagatcaccg
     1981 cgcgcagcga ctccgacagc atggagcgca ccatgtcctt ctcctcaccc gggaacacat
     2041 cgacgattcg atcgatggtc ttggccgcgg aggaggtatg cagagtgccg aacaccaggt
     2101 ggccggtttc ggcggcggtg agcgccaggc ggatggtctc caggtcgcgc atttcgccga
     2161 ccaggatcac gtccgggtct tcgcgcagcg ccgagcgcaa cgcctcggaa aaaccgagcg
     2221 tgtcacggtg cacctcgcgc tggttgacca ggcagcgctt cgactcgtgc acgaactcga
     2281 tcgggtcttc gatggtgatg atgtggtccg ggcggttatc gttgcagtga ttgaccatgc
     2341 cggcgagggt cgtggactta ccggaaccgg tgggaccggt gaccagcacc agcccgcgcg
     2401 gcagcatgca caggtcgcgg aagatcttgg gcgcgttcag atcatcgagc gacaggatgg
     2461 tcgaggggat gttgcggaac accgccccgg cgccgcggtt ctggttgaag gcgttgacgc
     2521 ggaaacgcgc cagcttcgga atctcgaagg agaagtcggt ctcgaagaac tcctcatagg
     2581 ccttgcgctg cttgtcgttc atgatgtcgt acaccatgga gtgcacttcc ttgtgcgaca
     2641 gcgccggcat gttgatgcgt ttcatgtcgc cgtccacccg gatcatcggc ggcacgcccg
     2701 aggacaggtg taggtccgag gcattgttct tgacggcaaa cgccaggagt tgcgcgatat
     2761 cgacggccat ggatgctccc gaacctaagc ctgcgggggc aggaagagcg acgtgcaagc
     2821 ctgcgccaca agcgcgccgg cagtatagcg gaggggtcac acgtccaata tgttaatgag
     2881 tccgcagaat ttgcccgcgg tgctgcacga agtgcgcgcg cgcatggcgc gggcggccgc
     2941 cgcagccggc cgtagtgcgc actcggtcac actccttgcg gtcggcaagg cacaaccggt
     3001 ggagctgctg gccgcggccg ccgactgcgg tgtcacggat tttggcgaga g
//
LOCUS       DATDMP010000131         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_686924, whole genome shotgun sequence.
ACCESSION   DATDMP010000131 DATDMP010000000
VERSION     DATDMP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686924"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..441
                     /locus_tag="VI195_02195"
     CDS             55..441
                     /locus_tag="VI195_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6823224.1"
                     /translation="MKRRLIDQIGDSPTFVAEGSCVTGDLETAGPLVVCGAVRGDGRV
                     GGALRMAVTAQWEGEIHAKAAVIAGKIIGKLVVEDKLELGMTAVIRADIVARSIAVAR
                     GAIIDGSVTVTSGEPVLEFEEKRAAT"
     gene            complement(447..1484)
                     /locus_tag="VI195_02200"
     CDS             complement(447..1484)
                     /locus_tag="VI195_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6823225.1"
                     /translation="MPTVDDDFRPARWLRNHHLQSMLASTAWRRARLLRESAPLLAAA
                     REVLLDCGAGVRLQCFISTPAQGKGTPVVLLHGWEGSATSLYVLSLARQLFERGCEVV
                     RLNLRDHGDTHHLNQELFHSCRLPEVIGAVAALQRLCAGRALHLVGFSLGGNFMLRVA
                     AQAGAAGLDLARVVAVSPVLEPVETLAALQRGMPGYEAYFVRKWLRSLRRKQQCWPDC
                     YDFTQLARLRDLKLMTAELVRHHSEFASLEEYLNGYAITGERLASLEVPASILTSLDD
                     PIIPASAVARLARPPSLSLTVTRYGGHCGFFERLRGPTWLERRILGLLGADAPQPLAA
                     EGTDIELREIG"
     gene            complement(1502..>2569)
                     /locus_tag="VI195_02205"
     CDS             complement(1502..>2569)
                     /locus_tag="VI195_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823226.1"
                     /translation="IPIMMYTSQEGEVYLGQARALGALGVLPKQIKPADVSKVLYQLH
                     LVPDRRTSEQSSFTALNVQSERAFGDAPARRALTDTTLREHFAELRRALVAGVDTQTD
                     RITAEVRALLLESLPPPSPLPAAEAPPPRPATVPWAWIVACAALLVAFTSSALWWRNV
                     KLLERLEVEVAHLQAASASAASNALPPGHATPATSRPAAGGAATDESKPIVVPVPYGA
                     EPLTGVRLDLIRQQLYRLARQKVTGVADIKTFAGRFCLLGSALEGYSPAPDEALFARC
                     DLVGNPSDEALTAGQRTPLALANLIGEIRSTTHGALEVQVSAGDPANVLVPYPPATAD
                     LVAGDWNRAAIANNRVEIRVH"
BASE COUNT          428 a          851 c          903 g          387 t
ORIGIN      
        1 ttatgacctc gcatcgaccc gggcgcaggc cgcggagctg gaatggccca aacattgaag
       61 cgcaggctga tcgaccagat tggcgactcg ccgacgttcg tcgccgaggg ctcctgcgtg
      121 accggcgatc tggagacggc aggtccgctc gtcgtgtgcg gcgccgttcg cggtgacggg
      181 cgggtcggag gggccctgcg catggccgtc accgcgcaat gggagggaga gattcacgcc
      241 aaggcggccg tgatcgccgg caagatcatc ggcaagcttg ttgtcgagga caagctcgag
      301 cttggcatga cggccgtgat tcgtgccgac atcgtcgcgc gcagcatcgc ggtggcgcgc
      361 ggggcgatca tcgatggttc ggtaaccgtt accagcggcg agccggtgct cgagttcgag
      421 gagaaacgcg cggcgacctg aatcgttcag ccgatctcgc gcagctcaat atcagtcccc
      481 tccgcagcga gcggttgcgg cgcgtccgcg ccgagcagac ccaggatgcg ccgctcgagc
      541 caggtcggcc cgcgcagccg ctcgaagaat ccgcaatggc cgccgtaacg cgtgacggtg
      601 agcgacagcg acggcggtcg ggcgagtctc gccacggcac tcgctggaat gatcggatca
      661 tcgagtgatg tcaggatgct cgcgggcacc tcgagacttg ccagccgctc accggtgatg
      721 gcatagccgt tcaggtactc ctccagcgag gcaaactcgc tgtggtgacg caccagctca
      781 gcggtcatca gcttcagatc acgcaggcgt gcgagctgcg tgaagtcgta gcagtcgggc
      841 cagcactgct gcttcctgcg caggctgcgc agccacttgc gcacgaagta ggcctcgtag
      901 cccggcattc cacgctgcag cgccgccagg gtctcgaccg gctcgagcac gggtgagacc
      961 gcgacgaccc ttgcgaggtc gaggccggcg gcaccggcct gcgctgcgac gcgcagcatg
     1021 aagttgccgc cgagcgagaa gcccaccagg tgcagcgccc ggcctgcgca gagccgttgc
     1081 aaggccgcga ccgcgccgat gacctccggc aggcggcagg agtggaacag ctcctggttc
     1141 agatgatgtg tgtcgccgtg atcgcgcaga ttgagccgaa ccacctcgca gccccgctcg
     1201 aacagctgcc gggcaagtga cagcacgtac agcgaggtgg cactgccttc ccagccgtgc
     1261 agcagcacca ccggggtacc cttgccttgc gccggcgtag agatgaaaca ctgcaggcgc
     1321 acacccgcgc cgcagtcgag cagcacttcg cgagccgccg cgagcagcgg cgccgactcc
     1381 cgcagcagcc gtgcgcgccg ccaggccgtg ctcgccagca tcgactgcag atggtggttg
     1441 cgcagccagc gtgctggccg gaaatcgtca tcgacggtcg gcacgggctg aaccgtcggt
     1501 ctcaatgcac gcggatctcg acccggttgt tcgcgatcgc ggcgcgattc cagtcaccgg
     1561 caacgaggtc ggccgtcgcc ggagggtaag gcacgagtac gtttgccggg tcgccggccg
     1621 agacctgcac ctcgagtgcg ccgtgcgtgg tgctgcgaat ctcgccgatg aggttggcga
     1681 gcgcgagagg agtccgctga ccggcggtga gcgcctcgtc cgaagggttg ccgaccaggt
     1741 cgcagcgcgc gaacaaggcc tcatcgggcg cgggtgagta gccctcgagc gccgagccca
     1801 gcagacagaa gcgtcccgca aaagtcttga tgtccgcaac gcccgtgacc ttctgtcgtg
     1861 caagccgata gagctgctgg cggatgagat ccagacgcac tccggtgagc ggctcggctc
     1921 cgtagggtac cgggaccacg atcggcttgc tctcgtccgt cgcggccccg ccggcggcgg
     1981 gcctggacgt cgcgggcgta gcgtgcccgg gcggcagcgc gttactcgcc gcactggccg
     2041 atgccgcctg cagatgcgcc acttcgacct cgagccgctc gaggagtttc acgttgcgcc
     2101 accagagcgc gctgctcgta aacgcaacga gcagggcagc gcaggccacg atccaggccc
     2161 acggcaccgt cgcgggacgc ggcgggggcg cctccgctgc cggcagtggc gagggcggtg
     2221 gcagtgactc cagcagcagc gcacgcacct cggcggtgat gcgatcggtc tgtgtatcga
     2281 cgcctgcgac cagtgcgcgc cgcagctcgg cgaaatgctc gcgcagggtg gtgtcggtga
     2341 gtgcgcgccg cgccggcgcg tcgccgaagg cacgctcgga ctgcacgttc agcgccgtga
     2401 agctcgactg ctcgctggtc ctgcgatcgg gcacgaggtg cagctggtag agcaccttgg
     2461 agacatcggc aggcttgatc tgctttggca gcacacccag tgcgccgagt gcgcgtgcct
     2521 gtcccagata cacctcgccc tcctgcgagg tgtacatcat gatcgggat
//
LOCUS       DATDMP010000132         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_353826, whole genome shotgun sequence.
ACCESSION   DATDMP010000132 DATDMP010000000
VERSION     DATDMP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353826"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1938
                     /gene="glgB"
                     /locus_tag="VI195_02210"
     CDS             49..1938
                     /gene="glgB"
                     /locus_tag="VI195_02210"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY6823227.1"
                     /translation="MLLTEQDIYHFREGTHFRAYEKLGAHPATRAEPTRATRFAVWAP
                     NASAVSVIGDFNHWRRGHARLSPRDDSSGIWEGVLEGVGPGALYKYHVVPRHGGHAVD
                     KSDPYAFRTELPPRTASVVWDLAYAWQDAQWMQRRASVNALGAPWSIYELHLGSWRRV
                     PEEHNRFLSYRELAHAIAGYASELGFTHVELLPVMEHPFYGSWGYQVTGYFAPTARYG
                     TPQDFMYLVDYLHQRGLGVILDWVPSHFPADEHGLALFDGTHLYEHADPRQGFHPEWN
                     SSIFNYGRAEVRNFLASNALFWLEKYHIDALRVDAVASMLYLDYGRRAGEWIPNRFGG
                     HENLEAVDFLKQLNLAAYRDHPDTQTIAEESTAWPMVSRPTYLGGLGFGLKWNLGWMH
                     DTLKYLQQDPVFRKYHHSRLTFSIWYAFSENFVLPLSHDEVVHGKGSLIGRMPGDAWQ
                     QFAGLRLLYGYMWTHPGKKLLFMGGEFAQRREWHHDESLEWHVLQQPLHAGVQRWVAD
                     LNRLYRLTPALHELDFSEAGFAWVDCADADISVISYLRMGSSGPPVLVVCNFTPVARA
                     HYQLGVPRAGRWRECLNSDASDYGGSGQGNLGAVFAEDVAAHGHAHSLRVRLPPLAVV
                     VLMPE"
     gene            1955..>3053
                     /locus_tag="VI195_02215"
     CDS             1955..>3053
                     /locus_tag="VI195_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEY6823228.1"
                     /translation="MSARRKQAANGDPLWFKDAVIYEVHVRAFFDANDDGVGDFVGLT
                     RKLEYIEDLGVNTIWLLPFYPSPGRDDGYDIADYHSIHPQYGTRTQFRAFVREAHRRN
                     LRVITELVLNHTSDAHPWFQAARLAPPASAKRDWYVWSDTPQRYSGTRIIFRDTETSN
                     WAWDPVARAYYWHRFFSHQPDLNFDNPQVLRAMLRVMDFWLRLGVDGFRLDAVPYLVE
                     REGTINENLRETHEIIRVIRRAISSHFGDRMLLAEANQWPEDVRDYFGDGRDECQMAY
                     HFPLMPRMYMAIAQEDRHPIVEIMEQTPDIPDTCQWAIFLRNHDELTLEMVTSRERDY
                     MYQAYAADARARLNLGIRRRLAPLLDNDPERI"
BASE COUNT          512 a         1040 c          962 g          539 t
ORIGIN      
        1 ccactatcaa gaatcgcatt ttcgtggtga ccccagtaga ctttcccggt gctgttgacg
       61 gagcaggaca tctatcattt tcgcgagggc actcatttcc gggcgtatga gaagcttggt
      121 gcccatcccg cgacgcgcgc cgagccaacg cgcgccacgc gctttgcggt gtgggcgccc
      181 aacgccagcg ccgtatcggt cattggcgat ttcaatcact ggcggcgcgg ccatgcgcgg
      241 ctgagccccc gcgatgattc ctcgggcatc tgggagggcg tgctggaggg tgttggcccg
      301 ggcgcgctct acaagtacca cgtagttccg cggcacggcg gccatgcggt tgacaagagc
      361 gacccgtacg cattccgcac cgagctgccg ccgcgcaccg cttcggtggt atgggatctc
      421 gcctacgcct ggcaggatgc gcagtggatg cagcggcgcg ccagcgtcaa cgcgctcggc
      481 gccccctggt cgatctacga gctgcatctc ggctcctggc ggcgcgtgcc ggaggagcac
      541 aaccgctttc tgagctatcg cgagctggcc cacgcgatcg ccggttatgc gagcgagctc
      601 ggcttcaccc acgtcgagct actgccggtg atggagcatc ccttctatgg ctcctggggg
      661 tatcaggtca cgggctattt cgcgccgacc gcgcgctatg gcacaccgca ggacttcatg
      721 tatctcgtcg actacctgca ccagcgcggg ctcggtgtga tcctcgactg ggtaccttcg
      781 cactttccgg ccgatgagca cggactcgcg ctcttcgatg gcacgcatct ctacgagcac
      841 gccgatccac gtcagggatt ccatcccgaa tggaacagct cgatcttcaa ctacgggcgc
      901 gccgaggtgc gcaatttcct cgcgagcaat gcgctgttct ggctggagaa gtatcacatc
      961 gacgcgctgc gcgtcgatgc ggtggcctcg atgctgtatc tcgattacgg ccgtcgtgcc
     1021 ggcgagtgga ttccgaaccg cttcggcggt cacgagaacc tcgaggccgt cgacttcctc
     1081 aagcagctga accttgccgc ttaccgcgat caccccgaca cccagaccat agccgaggag
     1141 tccaccgcgt ggccgatggt gtcgcgaccg acctacctcg gcggtctcgg cttcggcctc
     1201 aaatggaacc tgggctggat gcacgacacg ctcaagtatc tgcagcagga tccggtattc
     1261 cgcaagtacc accacagccg cctcaccttc agcatctggt acgccttcag tgagaacttc
     1321 gtgctgccgc tctcgcacga cgaggtagtg cacggcaagg ggtcgctcat cggccgcatg
     1381 cccggcgatg catggcagca gttcgccggg ctgcggctgc tgtacggtta catgtggacg
     1441 catcccggca agaaactgct cttcatgggc ggggagttcg cgcagcggcg cgagtggcac
     1501 catgacgaga gcctcgaatg gcacgtgctg cagcagccac tgcacgccgg ggtgcagcgc
     1561 tgggttgccg atctgaaccg tctctaccgg ctcaccccgg cgctgcacga gctcgacttc
     1621 agcgaggcgg gcttcgcctg ggtcgattgt gccgacgccg acatcagtgt gatctcctac
     1681 ctgcgcatgg gcagcagcgg cccgccggtg ctggtggtgt gtaacttcac cccggtagcg
     1741 cgcgcgcatt accagctcgg cgtgccgcgg gcgggccgct ggcgcgagtg tctcaacagc
     1801 gatgccagcg actacggcgg cagcggccag gggaacctgg gcgcggtgtt cgccgaagat
     1861 gtcgccgcgc acggccatgc gcactcgctg cgggtgcgac tgccgccgct ggcggtcgtc
     1921 gtgctgatgc cggagtagcc gggaccgcag ccgcatgagt gcgcgacgca agcaagccgc
     1981 gaacggggat ccgctgtggt tcaaggacgc ggtgatttac gaggtgcacg tacgcgcctt
     2041 cttcgacgcc aacgacgatg gcgtcggcga tttcgtgggg ctgacgcgca agctcgagta
     2101 catcgaagat ctcggcgtca acacgatctg gctgctgccg ttctacccct cccccggccg
     2161 tgatgacggc tacgacatcg ccgactatca cagcattcac ccgcagtacg gcacgcgcac
     2221 gcagttccgc gcgttcgtgc gcgaggcgca ccgccgcaac ctgcgcgtca tcaccgagct
     2281 ggtgctcaac cacacttccg acgcccaccc ctggttccag gcggcgcggc tcgctccgcc
     2341 cgcctcggcc aagcgcgact ggtacgtgtg gagcgacacc ccgcaacgct actccggcac
     2401 gcgcatcatc tttcgcgata ccgagacctc caactgggcc tgggacccgg tggcgcgcgc
     2461 ctactactgg caccgcttct tcagtcacca gccggatctg aacttcgaca acccgcaggt
     2521 gttgcgcgcc atgttgcgcg tgatggactt ctggctgcgg ctcggagtcg acggcttccg
     2581 cctggacgcg gtgccgtacc tggtcgagcg cgaaggcacc atcaacgaga acctgcgcga
     2641 gacgcacgag atcatccgcg tcatccgccg cgcgatctcc agccacttcg gcgatcgcat
     2701 gctgctggcg gaagccaacc agtggccgga ggacgtgcgc gactacttcg gcgacggccg
     2761 cgatgagtgc cagatggcct accactttcc cttgatgccg cgcatgtaca tggcgattgc
     2821 gcaggaggac cgccacccga tcgtcgagat catggagcag accccggaca tccccgacac
     2881 ctgccagtgg gcgatcttcc tgcgcaatca cgatgagctg acgctcgaga tggtcacgag
     2941 ccgcgagcgc gactacatgt atcaggcgta cgcagccgac gcgcgcgccc ggctcaacct
     3001 cggcatccgc cgccggctcg ccccgctgct cgacaacgat cccgagcgca tca
//
LOCUS       DATDMP010000133         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_242715, whole genome shotgun sequence.
ACCESSION   DATDMP010000133 DATDMP010000000
VERSION     DATDMP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242715"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VI195_02220"
     CDS             <1..218
                     /locus_tag="VI195_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823229.1"
                     /translation="DLEAHVRRILEFCGLEFEPACLEYYKTQRRIHTVSAEQVRRPVS
                     REGIDQWRNFDPWLGPLREALGPLADR"
     gene            297..1268
                     /locus_tag="VI195_02225"
     CDS             297..1268
                     /locus_tag="VI195_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY6823230.1"
                     /translation="MSGAHADPPAAQPAARLGSFDIEAHRGGRALRPENTLASFANAL
                     SMGVDTLELDMGVTKDGVIVVSHERGLNPDLARAPDGRYITTGIPYVKLTLAEVKKYD
                     VGQIRPGSDYAARFPEQRAIPGTRIPTLAEVFDLVRRSGDRHVRLNIETKIDPTHPDE
                     SLDPQRFVTAVLDLLRREHFTERVMIESFDWRTLLLVQKQAPGIPTVYLTQQQQPEEN
                     IYLDKSSPWTAGFDPAKYGGSVPRAVKAAGGSIWSPLYEDVTPGSIATAHSLGLPVIV
                     WTVNEPKDMSRLIDMGVDGIISDRPDLLRAVAASKGIALPSPTPVSP"
     gene            1278..2762
                     /gene="glpK"
                     /locus_tag="VI195_02230"
     CDS             1278..2762
                     /gene="glpK"
                     /locus_tag="VI195_02230"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY6823231.1"
                     /translation="MSEPQHVIAIDQGTTSTRAIIFDAAARATAIERRALTQHYGEAG
                     RVEHDPEEIWRDTEATVREVIARCPGGAAGVAALGITNQRETVVVWERASGTPIHPAI
                     VWQDRRTAQECARLKAEGVEDLVRRKTGLLLDPYFSATKIAWILDAVPGARTRAERGQ
                     LAFGTIDCFLLWRLTGGRVHATDVTNAARTLLYDIHAQCWDEELLRLLRVPRALLPEV
                     RDSSEVYGTTSLFGRALPIAGIAGDQQAALIGQACFRPGMAKSTYGTGCFLLLNTGEV
                     AVTSANRLLTTPAYRIGGRIAYALEGSIFVAGAAVKWLRDGLKVIESASQTAALAARL
                     PYDHGVYLVPAFVGLGAPHWEPNARALLCGLSFDTAAAHLARAALEAVAYQTLDLTLA
                     MQRDGAQPLAALRIDGGMAANEWLCQFLADILQARVERPGEIETTALGAAFLAGLATG
                     VWPGLDALSSRWSTVAVYEPRMNRAQRAALIEGWQQALSRALLA"
BASE COUNT          454 a          972 c          913 g          455 t
ORIGIN      
        1 aggacctcga ggcgcacgtg cgccgcattc tggagttctg cgggctagag ttcgagccgg
       61 cctgcctcga gtactacaag acgcagcggc gcatccatac cgtgtccgcc gagcaggtgc
      121 gccgtccggt gagccgcgag ggaatcgatc agtggcggaa tttcgatccc tggctcggac
      181 cgctcaggga ggcgctgggc ccgctcgccg accgatagcg ctgcctcgac ctgccaggtg
      241 tgacacgcgt gcatcgatcc gctctgaagt cgtctctgct cctcctgtgc ctctgcatgt
      301 caggcgctca tgctgatcca cctgcggccc agcctgccgc gcgcctcggc tcattcgata
      361 tcgaggcgca ccgtggcggg cgagcgttgc gcccggaaaa caccctggca tcatttgcca
      421 acgccctgtc gatgggcgtg gatacactcg aactcgacat gggcgtgacc aaggacgggg
      481 tcatcgtcgt gtcgcacgag cgtgggttga atccggatct ggcgcgcgct ccggacgggc
      541 gctacatcac gaccggtata ccttacgtca agctgaccct ggcggaagtc aagaagtacg
      601 acgtgggcca gattcgtccg ggcagcgact acgctgcccg atttcccgag cagcgcgcca
      661 tacccggcac tcgaatcccg acccttgcgg aagttttcga cctggtgcgc cgctcgggcg
      721 accgccacgt gcggctcaac atcgaaacca agatcgatcc gacgcacccc gacgagtcgc
      781 tcgatcccca gcgcttcgtg actgcggtac tcgacctgtt gcgccgcgag cacttcacgg
      841 agcgcgtcat gatcgagtcg ttcgattggc gcaccctgct gctggtgcag aagcaggccc
      901 ccgggatccc gaccgtctat ctcacccaac agcagcagcc cgaagagaac atctatctcg
      961 acaagagctc gccctggacg gccgggttcg atccggccaa gtacggcgga tcggtacccc
     1021 gtgccgtcaa ggccgcaggg ggctccatct ggtcgcccct ctacgaggac gtgaccccgg
     1081 gttcgatcgc cacagcacac agcctcggcc tgccggtcat cgtctggacg gtcaacgagc
     1141 cgaaggacat gagccgtctc atcgacatgg gcgtcgatgg aatcatctcg gatcgaccgg
     1201 acctgctgcg cgcggtggcc gccagcaagg gcatcgccct cccgtcaccg actcccgtca
     1261 gtccgtgaac aaacggcatg agtgagccgc agcacgtcat cgccatcgat cagggcacca
     1321 cctccacgcg agccatcatc ttcgatgcgg cggcgcgggc gacggcgatc gagcggcgcg
     1381 cgctgacgca gcactacggc gaagcgggcc gcgtagagca cgatccggag gagatctggc
     1441 gcgacaccga agccaccgtg cgcgaggtca tcgcccgctg cccggggggg gccgccgggg
     1501 tggccgcgct cggcatcacc aatcagcgtg agacggtcgt ggtgtgggaa cgtgccagcg
     1561 gcacgcccat ccatccggca atcgtctggc aggaccggcg caccgctcag gagtgcgcgc
     1621 ggctcaaggc cgagggtgtg gaggatctgg tacggcgcaa gaccggcctg ctgctcgatc
     1681 cttacttctc cgctaccaag atcgcctgga tactcgatgc ggtgcccggc gcgcgcacgc
     1741 gcgccgagcg cggtcagctc gccttcggaa ccatcgactg ctttctcctg tggcgcctca
     1801 ccggtggccg cgtgcacgcg acggatgtca ccaacgccgc gcgcacactg ctctatgaca
     1861 ttcacgcgca gtgctgggac gaggagttgc tgcgcctgct gcgcgtaccg cgcgcgctgc
     1921 tccccgaggt acgcgacagc agcgaggtgt acggcacgac ttccctcttc ggccgcgcgc
     1981 tgccgatcgc cggcatcgcc ggcgatcagc aggcagcgct catcgggcag gcgtgcttca
     2041 ggccggggat ggcgaagtcc acctacggta ccggttgttt tctgctactg aacacgggcg
     2101 aggtggcagt gacctcggcc aaccggctgc tcacgacgcc ggcctaccgc atcggcgggc
     2161 ggatcgctta tgcgctcgaa ggctcgatct tcgtcgcggg agccgcggtg aaatggctgc
     2221 gcgatggact gaaggtgatc gaaagcgcct cacagaccgc cgcgctcgct gcccgcctcc
     2281 cctatgacca cggggtctac ctcgtaccgg ccttcgtcgg gctcggcgct ccgcactggg
     2341 agccgaacgc gcgcgctcta ctgtgcggac tctctttcga tactgccgcc gcgcacctcg
     2401 cgcgcgccgc gctcgaggcg gtcgcatacc agaccctcga tctcacgctc gccatgcagc
     2461 gcgatggtgc gcagccactt gcggcgttgc gcatcgatgg cgggatggcg gcgaacgagt
     2521 ggctctgcca gtttctcgcc gacattctgc aggcgcgagt cgagcgtccg ggggagatcg
     2581 agaccaccgc actcggcgct gccttcctcg ccggcctcgc caccggcgta tggcccggcc
     2641 tcgatgcgtt gtcatcccgc tggagcacgg tcgcggtgta cgagccgcgc atgaatcgcg
     2701 cgcagcgcgc cgccctcatc gagggatggc agcaggcgct gtcgcgcgcg ctgctcgctt
     2761 aagccgaggg accgggccct aacgacgcgg aggc
//
LOCUS       DATDMP010000134         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1242664, whole genome shotgun sequence.
ACCESSION   DATDMP010000134 DATDMP010000000
VERSION     DATDMP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242664"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VI195_02235"
     CDS             complement(<1..1185)
                     /locus_tag="VI195_02235"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY6823232.1"
                     /translation="MTTGGDAPHLSPTLVGRLERLIFGHRTAILALFTLGTVLLGATA
                     VRGLRIDTSFNKTLPIHHEYMRTYLDPRVAEFRGADRVLIALIARDGNMFTPQFFSAL
                     RKATDEVILMDGIDRTQVQSIFTPNVRYLEVVEDGIEAGNVIPADFTPTPENMARVRD
                     NIRKAGIIGRLVANDFSGALVSAIVLDSDAQGKPVDPIRIAHELERRVRAPLEGHGIE
                     VHIIGFAKVVGDIADGAASVLLFAVITLVLTLLAVRIYCQSWRVAFVPVLCSGVAVLW
                     QLGALVLLRYGIDPIGLLVPFLIFAIGVSHGVQKISAVSDAAVDGLDSLQAARRTFRQ
                     LLAPAIIALLADLVGFVTILVIPVQVIREMAITASIGVAIVIVTDLVLLPVLVSYVSF
                     DAG"
     gene            complement(1182..>2252)
                     /locus_tag="VI195_02240"
     CDS             complement(1182..>2252)
                     /locus_tag="VI195_02240"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY6823233.1"
                     /translation="GSAAALAVVLAALALAAGAQPPAPQPLPRSAEHARLAAHTLLIA
                     LAAAGERLVAVGDRGVIVLSDDRGASWTQAASVPVQALLTGVCFLDAQHGVAVGHDEV
                     ILTTADGGGHWQRTHYAPEAQRPLLDVWCGTGGQAIAVGAYSAYFTSADGGASWHEVK
                     FTPAAPAARSRERAPAPPPAAASGAADEQGAAGGYHLNRIAGGGAGRLYIAAEAGHLY
                     ASDDSGASWRTLASPYEGSFFGVLPLPGEALLAFGLRGHLYRSADAGASWQAIETGTV
                     AMLTGAARFGPDAIAVVGLSGVILVSRNGGRSFALQQQADRSGLSGLSPAGDARLAVV
                     GEEGARLIVLADPGRVAASVPR"
BASE COUNT          379 a          783 c          785 g          305 t
ORIGIN      
        1 gccggcatcg aaggacacgt atgagaccag taccggcagc agcactaggt cggtgacgat
       61 gacgatggcg acgccgatgc tcgcggtgat cgccatctcg cggatcacct gcacgggtat
      121 cacgagaatg gtgacgaagc ccaccaagtc cgcgagcagc gcgatgatcg ccggcgccaa
      181 cagctgccgg aacgtgcggc gcgcggcctg caggctgtcg aggccgtcga ccgctgcatc
      241 gctcaccgcg ctgatcttct gcacgccgtg actcacgccg atggcgaata tcaggaacgg
      301 caccagcagc ccgatgggat cgattccgta gcgcagcagc accagcgccc cgagctgcca
      361 cagcaccgcg acgcctgagc agagcaccgg cacgaaggcg acccgccacg actggcagta
      421 gatgcgcacc gccagcagcg tcagaacgag cgtgataacc gcaaacagca gcacggacgc
      481 ggcgccatcg gcgatgtcgc ccacgacctt ggcgaagccg atgatgtgca cctcgatgcc
      541 gtgaccctcg agcggtgcgc gtacgcgccg ctcgagctcg tgggcgatgc ggatcggatc
      601 gaccggcttg ccctgcgcgt cgctgtcgag cacgatggcg ctcaccagcg cgccggaaaa
      661 gtcattcgcc accagccgcc cgatgatgcc ggccttcctg atgttatcgc gcacccgtgc
      721 catgttctcc ggcgtggggg tgaagtctgc cgggatcaca ttaccggctt cgatgccgtc
      781 ctcgaccacc tcgaggtagc gcacgttggg agtgaagatc gactgcacct gtgtgcggtc
      841 gatgccatcc atgagaatga cctcgtcggt agccttgcgc agcgcgctga agaactgcgg
      901 cgtgaacatg ttgccgtcgc gtgcgatcag tgcgatcaac acgcggtcgg cgccacgaaa
      961 ctcggccacg cgcgggtcga ggtaggtgcg catgtattcg tgatggatgg gaagagtctt
     1021 gttgaacgag gtgtcgatgc gcagtccgcg cacggcggtc gcgccgagca gcacggtgcc
     1081 gagcgtgaac agggcgagga tcgccgtgcg gtggccgaag atcagccgct cgagccggcc
     1141 gaccagcgtc ggggacagat gcggcgcgtc accgccggtg gtcatcgggg cacgctcgcg
     1201 gcaacgcggc ccggatctgc gagcacgatg agccttgccc cctcctcgcc caccaccgcg
     1261 agccgcgcat cgcctgccgg gctgaggcca gacagcccgg aacggtctgc ctgctgttgc
     1321 agagcgaaac tgcggccgcc gttgcggctc acgagtatga cgcccgagag cccgaccacg
     1381 gcgatggcgt ccgggccgaa gcgcgccgcg ccggtgagca tcgccacggt gcccgtctcg
     1441 atcgcttgcc agctcgcacc ggcatccgcg gagcggtaaa ggtggccgcg caggccgaag
     1501 gcgagcagcg cctcacctgg gagcggcagc accccgaaga acgagccctc gtacggggag
     1561 gcgagcgtgc gccagctcgc accgctatca tcggaagcgt agagatgccc cgcttccgcc
     1621 gcgatgtaca gtcgccccgc gccgccaccg gcgatgcgat tgaggtggta gccgccggcc
     1681 gcgccctgct cgtccgccgc gcctgaggcc gccgctggag gaggcgcggg cgcgcgctcg
     1741 cggctgcgcg ccgcgggcgc tgccggagtg aatttcacct cgtgccagct cgccccgcca
     1801 tcggcgctgg tgaagtatgc gctgtaggcc cccacggcaa tcgcctgccc gcccgtgccg
     1861 caccagacat cgagcaatgg ccgctgtgcc tcgggagcat agtgcgtgcg ttgccagtga
     1921 ccgcctccgt cggcagtggt gaggatgacc tcatcgtgcc cgaccgccac accatgctgc
     1981 gcatcgagaa agcacacgcc ggtgagcagc gcctgcacgg gtacgctcgc cgcctgcgtc
     2041 cagctcgcgc cgcggtcgtc ggagagcacg atgacaccgc ggtccccgac cgccaccagc
     2101 cgctcgccgg cggcggcgag ggcgatgaga agcgtatggg cggcgagccg cgcgtgctcg
     2161 gcgctccggg gtagcggctg cggcgccggc ggctgcgccc cagcggccag ggcaagcgcc
     2221 gcgagcacga cggcgagcgc agcggcggag cc
//
LOCUS       DATDMP010000135         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1798145, whole genome shotgun sequence.
ACCESSION   DATDMP010000135 DATDMP010000000
VERSION     DATDMP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798145"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VI195_02245"
     CDS             <1..1063
                     /locus_tag="VI195_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY6823234.1"
                     /translation="DLGAQSWANESWRTGGAPVWGWMSYDPQLDLIYYGSGNAAPYNA
                     EQRSGDNKWSASVLARRPEDGALVWAYQFTPHDSWDYDATGAMVLADLTVAGQAVKAL
                     VHFDKNGFAYTFDRATGRVLVAKPFTTVNWAESVDLSNGRPVLNPAKQTGAARGNVKE
                     ICPSLEGGVSPASPPAYSPRTHLFYTATNNFCMDYEVRPARRLKGTPFMGVTSPYSAG
                     AGGYLGSFMAWDAASGRKVWENREPFPNWSGALVTAGDVVFYGTLDGWFKSADARTGR
                     VLSKFKVGSGVVGNPITYRSPDGRQHVAVYAGFGGDFALIAGDARSADPDDIRAPADP
                     VKDIARHTSQGGIVWIFGL"
     gene            1060..1569
                     /locus_tag="VI195_02250"
     CDS             1060..1569
                     /locus_tag="VI195_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6823235.1"
                     /translation="MNTHLAEDLERQRPVRVARRHAPLVILAGALLSCGRPHSAPEQP
                     ATVPPAVAYEAHLVAGDTPPPSGSLRNPHAGDSAVARSGAVLFNAMNCDGCHGGDGSG
                     WVGPNLGDGRWRYGAADAEVFSSIYYGRPRGMPAFGAVLGSEGVWTLVTYLRSLSAPP
                     DAPTESWQN"
     gene            1643..2197
                     /locus_tag="VI195_02255"
     CDS             1643..2197
                     /locus_tag="VI195_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY6823236.1"
                     /translation="MKPHVPFELAVPVGAVDHVIGAPHAVATVVEYGDFECPNCKQAQ
                     PAVKMLLERFTGRVRFVFRHFPLEEVHPHSLAAAQAAECAAGQGRFWEMHDVLFANQE
                     HLKAADLRHYAERLELDMARYIAEMDDQVYLQRVREQLQSGLDSGVRATPTFFVNGRI
                     EDVSFGLRALFDVVEGVLQRSGSR"
BASE COUNT          383 a          684 c          788 g          382 t
ORIGIN      
        1 ggatcttgga gcgcagagct gggccaacga gagctggcgt accggcggtg cgccggtgtg
       61 gggatggatg tcgtacgatc cgcagctgga tctcatctat tacggctccg gcaacgccgc
      121 tccctacaac gcggagcaac gctccggcga caacaagtgg agtgccagcg tgctcgcccg
      181 gcggccggag gatggcgcgc tcgtatgggc gtatcaattc acgccacacg acagctggga
      241 ctacgacgcg accggtgcga tggtgctggc ggacctgacg gtcgcgggac aagcggtcaa
      301 ggccctggtg cacttcgaca agaacggttt cgcctatacg ttcgaccgcg caactgggcg
      361 cgtgctggtc gccaagccct tcacgacagt gaactgggcc gaatcggtgg atttgagcaa
      421 cggtcggccg gtgctgaacc cggcaaagca gaccggcgct gcgcgcggca acgtcaagga
      481 gatctgcccg agcctggagg gaggtgtcag ccccgcctcg ccgccggcat attcgccacg
      541 cacccatctg ttctacaccg ccaccaacaa tttctgcatg gactatgagg tgcgaccggc
      601 acgacggctg aagggcacgc cgttcatggg cgtcaccagt ccctacagcg caggcgccgg
      661 cggttacctg ggcagcttca tggcatggga tgcggcgagc ggcaggaagg tgtgggagaa
      721 cagggagcct ttccccaact ggagcggcgc actcgtgact gccggggatg tcgttttcta
      781 cggcacgctc gatggctggt tcaagtcggc cgacgcgcgc accggacggg tgctgtcgaa
      841 gttcaaggtc ggctcgggtg tggtcggtaa tccaatcacc taccgcagcc cggacggccg
      901 gcagcatgtt gcggtgtacg ccgggttcgg tggcgacttt gcgctgatcg caggcgatgc
      961 gcgctccgct gatccggatg acatccgcgc gccggcggac cccgtgaagg acatcgcgcg
     1021 tcacaccagc cagggcggca tcgtctggat tttcggccta tgaatacgca cctcgcggaa
     1081 gacctcgaac gtcaacggcc cgtgcgcgtg gctcgccggc acgcgccact ggtgatcttg
     1141 gctggagcgc tgctgtcctg cgggcgccca cattccgcgc cggagcagcc cgccacggta
     1201 ccgcctgccg tagcgtacga ggcgcacctg gtggccggag atactccgcc gccgagtggc
     1261 tcgctgcgca atccgcacgc tggcgatagc gcggtggcac gcagcggcgc ggtactgttc
     1321 aacgccatga attgcgatgg ctgtcatggg ggcgatggct cgggatgggt cggaccgaat
     1381 cttggagatg ggcgctggcg ttatggcgct gcggatgccg aggtgttcag ctccatctac
     1441 tacggccgtc cacgcggcat gccggcgttc ggcgcagtac tgggctccga gggtgtatgg
     1501 acgctcgtca cctatctgcg ctcgttgtcc gcgccgcctg acgcaccgac tgagtcttgg
     1561 cagaactgac aagctcgagc gaagcggcga cgtgaccggg tgcgccggaa gagcgcatag
     1621 tggcaggtga gcgaggtgtg tcatgaagcc gcacgtaccc ttcgagctcg cagtgcccgt
     1681 tggagctgtc gatcacgtga tcggggcgcc gcacgcagta gccaccgtgg tcgagtacgg
     1741 cgacttcgag tgcccgaact gcaagcaggc gcagccagcc gtgaagatgc tgctcgagcg
     1801 cttcaccgga cgagtgcggt tcgtgttccg gcatttcccg ctcgaagagg tgcacccgca
     1861 ctcgctcgcc gcggcacagg cggcagagtg cgccgccggg caagggaggt tctgggagat
     1921 gcacgatgtg ctgttcgcca accaggagca cctgaaggcg gcggacctgc gccactatgc
     1981 cgagcggctc gagctcgaca tggcgcgtta catcgcggag atggatgacc aggtttacct
     2041 gcagcgcgtg cgcgagcagc tgcagagtgg gctcgacagc ggcgtgcgtg ccacgccgac
     2101 attcttcgtt aacggacgca tcgaggacgt gtccttcggt ctacgggcgc tttttgacgt
     2161 ggtggaaggc gtgttgcagc gaagcgggag ccgttagtca ggctccgaaa gcggtggggg
     2221 acgcggcttc catgaac
//
LOCUS       DATDMP010000136         2479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2132003, whole genome shotgun sequence.
ACCESSION   DATDMP010000136 DATDMP010000000
VERSION     DATDMP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132003"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VI195_02260"
     CDS             complement(<1..335)
                     /locus_tag="VI195_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEY6823237.1"
                     /translation="MKAASEKTVVAVAGVRDKDRGKHPATVARKTAAALSARASVPHP
                     RMNDVARAPDGKLPGVKLNADKRPLGLERRVPVMPEDRQSQLKLLIARGKEQGYLTYA
                     QVNDHLPSE"
     gene            complement(492..2339)
                     /gene="dnaG"
                     /locus_tag="VI195_02265"
     CDS             complement(492..2339)
                     /gene="dnaG"
                     /locus_tag="VI195_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY6823238.1"
                     /translation="MGRIPQHFIDELIARTDIVELIGTRVPLKKSGREYKACCPFHAE
                     KTPSFWVSPDKQFYHCFGCGKHGTALGFLMDHDHLAFPEAVEELATRLAMEVPHEGGA
                     DRSARPAEEPLYELMARVAHFYREQLAREPRARDYLAKRGLTEATLERFGIGYAPNSW
                     NELLRRFGTSDGGRKRLAEAGLIVERERGSDRESERYYDRFRDRVMFPIRDARGRVIA
                     FGGRIIDAGEPKYLNSPETTLFHKGRELYGLYETRRTRRALDRLVIVEGYMDAVSLHQ
                     AGVDYAVATLGTATTAEHLRRIFRLVPTVVFAFDGDRAGRAAAWRALQQALPETREGR
                     EIRFLFLPQGHDPDTLILAEGHTAFEQRLAAAVPLSEYLVRELSEQSELGSADGRARF
                     AEQARPLFARVPEGVYRELLLERLAQVIGLSAERLEALWTAGRTGVAATPASPSPARR
                     AAPARPARAAGRGSLVRQAVIRLLHYPAIALEVTAAERAGLDASEEPGVALLRELLDN
                     LREQPAQIPAQVIQRWMQHKDGETLQKLLAREEVITGAAVAAGELRSALVKLADQAAG
                     MRLQALEAKSRAGSLAGDELLEFQRLIERLTHRDARGRINEFGDSSDVK"
BASE COUNT          382 a          815 c          832 g          450 t
ORIGIN      
        1 atctcggagg gcaggtgatc gttcacctgc gcgtaggtga gataaccctg ttccttgccg
       61 cgggcgatca gcagtttcag ctgcgactgc cgatcttcgg gcatgaccgg cacgcgccgc
      121 tcgaggccga gcggacgctt gtcagcattg agcttgacgc ccggcaactt gccgtcgggg
      181 gcccgggcga catcgttcat ccgcgggtgc gggactgacg cacgcgcgct gagggcggcg
      241 gctgtcttgc gcgccacggt tgccgggtgc ttgccacgat ccttgtcgcg cactccggcc
      301 accgcaacga ccgtcttctc tgaggccgcc ttcacggctc tgctgctcgg cgctaccggt
      361 cgcttcttgt gcggattgtg gggcttgtgc ttgcgtgtca tgtgtggcgg cggggggtta
      421 cggcccgggt ttcccctatg tgctgcccgg actgggcagg ctcaaggggg ctaaaggcaa
      481 agcccgcaat tttatttgac gtctgatgaa tcgccaaatt catttatccg gccgcgggca
      541 tcacggtgag tcagtctctc gataagtctt tgaaattcca aaagttcatc accagcgagg
      601 ctcccggccc tgctcttggc ttcgagcgcc tggaggcgca ttcccgccgc ctgatcggcg
      661 agcttcacga gcgccgaccg tagctctcct gccgcgacgg ccgccccggt aatgacctct
      721 tcgcgtgcca ggagtttctg cagcgtctca ccgtctttat gttgcatcca acgctggatc
      781 acctgagcag gaatctgtgc cggctgctcg cgcaggttat cgagcagctc gcgcagcagc
      841 gcgaccccgg gctcttcgct cgcatcgaga ccggcgcgtt cggcagccgt gacctcgagc
      901 gcgatcgccg ggtaatgcag cagccgtatc accgcctgcc gtacgaggct gccgcggccg
      961 gctgcgcgcg cgggccgtgc cggtgccgcg cgacgtgcag gtgagggtga ggcgggcgtc
     1021 gccgctaccc cggttcggcc agccgtccac aacgcctcga gtcgttcggc ggagagaccg
     1081 atgacctgtg ccagccgctc gagcagcagc tcgcgataga caccttcggg cacgcgggcg
     1141 aacagcggcc gggcctgctc cgcaaagcgc gcacgcccgt cggccgagcc gagctcgctc
     1201 tgctcggaaa gctcgcgcac cagatattcc gacaacggca ccgctgcagc gagccgctgc
     1261 tcgaatgccg tgtgaccttc ggcaaggatg agcgtatccg gatcgtgacc ctgcggcagg
     1321 aagagaaagc ggatctcgcg tccctcgcgc gtctcgggca gggcctgctg cagagcgcgc
     1381 cacgcggcgg cgcgtccggc gcgatccccg tcgaaggcaa ataccaccgt cggcacgaga
     1441 cggaagatgc gccgcaagtg ctcggcggtg gtcgcggtgc cgagcgtcgc aacggcgtaa
     1501 tcgacgcccg cctgatggag gctcaccgca tccatgtagc cctcgacgat cacgagcctg
     1561 tcgagggcgc ggcgcgtgcg gcgcgtctcg tagagaccgt acagctcgcg tcccttgtgg
     1621 aacagcgtgg tttcaggtga gttgagatac ttcggctcgc cggcgtcgat gatgcgtccg
     1681 ccgaaagcga tgacgcgccc gcgggcatcg cggatgggga acatcacccg gtcgcgaaag
     1741 cgatcgtagt agcgctcgct ctcgcggtcg ctaccgcgct cgcgctcgac gatgaggcct
     1801 gcctccgcca ggcgtttacg cccgccatcg ctcgtgccga agcgccgcag aagctcgttc
     1861 caggagttgg gtgcgtagcc gatcccgaaa cgctcgagcg ttgcttcggt gagcccgcgc
     1921 ttcgccagat agtcacgcgc ccgaggctcg cgcgcgagct gctcgcggta gaagtgtgcg
     1981 acgcgcgcca tcagctcgta gagcggctcc tcggcaggcc gcgcgctgcg atcggcgccg
     2041 ccttcgtgcg gtacctccat tgcgaggcgc gtcgcgagtt cctccaccgc ttccgggaag
     2101 gccaggtgat cgtggtccat gagaaagccg agcgcggtgc cgtgcttgcc gcagccgaag
     2161 cagtgataga actgcttatc gggactcacc cagaaggaag gagtcttctc ggcgtggaat
     2221 ggacagcagg ccttgtactc gcggccggat ttcttcaacg gcacacgcgt gccgatgagc
     2281 tcgacgatat cggtacgggc aatgagctcg tcgatgaagt gctgcgggat gcgtcccaag
     2341 tggcgcttcc ggcgacgcgg cgggtcagcc gagcttctgc ttgatccgcg cgctgagcgc
     2401 gcccatgtcc gtgcggcctt gggctttcgg tttcacgaaa cccatgacct tgcccatgtc
     2461 cttgatggat gcggctccg
//
LOCUS       DATDMP010000137         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1020890, whole genome shotgun sequence.
ACCESSION   DATDMP010000137 DATDMP010000000
VERSION     DATDMP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020890"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1256..1621)
                     /locus_tag="VI195_02270"
     CDS             complement(1256..1621)
                     /locus_tag="VI195_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823239.1"
                     /translation="MEDHFLASFRREVDPKYWPKGGERGVFALTLLCLAFCSAAAGLV
                     NGTNEATSMQVIDFIKIWLGRLPGLPGRRYRVRAAALFSLFRHGLVHQCVPGRLDLED
                     GRTLGWILSRDGKGVSIFA"
     gene            complement(1980..>2759)
                     /locus_tag="VI195_02275"
     CDS             complement(1980..>2759)
                     /locus_tag="VI195_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6823240.1"
                     /translation="TCEEERMLKILLARARQGHRTAAYPLQPPALPDRFRGLPVLEPS
                     RCADGCQACAEACPTGAISLDGGLSLDMGKCLFCPDCVDACPEGAISFTSGHSLAARK
                     REDLIVRGKEYPRAAALDDKLRRLFGRSLKLRVVSAGGCAGCEADVNVLGTPVFDLGR
                     FGIQYVASPRHADGLLITGAVSENMRLALKKTYDAIPPPKIVIAVGACAVNGGPYIDH
                     PEVHNGAASTVPVDLFVLGCPPHPYTILDGLLRLLGRIEGR"
BASE COUNT          471 a          913 c          892 g          483 t
ORIGIN      
        1 tagagcgtgt cgccgtcgag agtggcgtag tcgaagcggc tggcgggacc cgggagcggc
       61 acgtcggcca ccttgcgcag gacgtcggat ccggcggcgc gggatggaca ggccgcgaag
      121 aggagcagca ggggagcgaa acggcgcatg aggctctcct cggatggatg atatgagtgt
      181 atcgtacccg gaatcctcgt ggcacagctg cgacattcgc cggaggggcc tccgcaacct
      241 caagaagcgc gccgagtcgc gctgcagttg accgcgctcg cggttcgggt ttcgccgcga
      301 cgctgtcggt gaccggggtg gggtgcggct tcagacatgc agaaatcgcg ttcgcgagac
      361 gtcgcggcgc ccgtcacggt gttcaaggcg atttctgaac tcgccgcagc cccaccccgg
      421 tccccgccag ctcttgcgca ccatgctccg gaggacgagg gccaccgcgc agcagatgtt
      481 ttggcgacca ttccggcttc gagtgaccat tgcaatatga tttgattcgg gatatattcc
      541 gagtcaatga atcggtcacc acagatcgaa ctgcctctgc gttttcgcgg tggacggcga
      601 aagggcgctg gaaggaagcc gaagggccga aaggcgcggg tctcgcatgc gccccgcgta
      661 cggttcaatc ggccgacgcc ggtgcatgtc gcgatgcgcg ttcgcgagga cgtctggaac
      721 ctgcgcagcc gccgcgcttg gcgccgcctg cgcggtgctg ccgactccaa ccgaactggt
      781 cagcgcaatc cgctacgtcc tcggcaatca cacccaccat ttcggagcga gcggcgtcga
      841 cccatacagc tccgctgcgc tttcgccgat ggaccgagcg gcgcttctcg cgcctgcgaa
      901 cggctggcta ctgcgcagcg gttggcatcg ggctctccag ggcgggttga tctgcgaagg
      961 ggacggttcc gtatgcgtca gatgagcatc tgatggtacc tgctcctccg caggcatcct
     1021 ctgatctcgc caagtatggc gtttttcgtg ttcggtgacc ccttgaggcg gcgaggcccg
     1081 tcggcgagta cgcttccgtc gtagatatgt gcagcgagaa tcgcatcctt gctgcactcg
     1141 tcgcggattt cctggaacgc tcggaggcaa tcccggaaga gcacatcggg ttcgacggag
     1201 atcctgtaat ggccaccgtg cttccccgtt gccgtcaagt agcccctgga tttcgtcacg
     1261 cgaagatgct cacgcctttt ccatcgcgac tgagaatcca tcccagcgtg cgcccatcct
     1321 ccaagtcgag cctgccaggg acgcattggt gcacgagtcc gtgcctgaac agcgaaaata
     1381 gagcagccgc tctcactcgg tatcttctcc cgggtaagcc gggtagccgg ccgagccaga
     1441 tcttgatgaa gtcgatcacc tgcatcgacg tcgcttcgtt tgtgccgttc acgagccctg
     1501 cggctgccga acagaaggcc agacagagca gcgtcaacgc gaacacgccg cgctcgccgc
     1561 ctttgggcca atatttcggg tcgacttcgc gacgaaatga ggcaaggaag tggtcttcca
     1621 tccacttcgc aacgtgttcc ggagcttgcg accaaggtgt gcggtccata ctcgtatgct
     1681 ttcgcaggtt ctcgcactct gcgccattca ctccgtgtga ttgatgccag gcaaacgcag
     1741 gagcgtttga ggttgcatgc ctttgcgcgg ctcccaagac acctgctgcg cggttgcgct
     1801 ggtctgtccg agcctgccgc gcgcagctag cggcggtgac cggggtgggc gggcggcgag
     1861 ttcagaaatc gcctggaacg ccgcgacgag cgccggcccg tcttccgaac gcgatttctg
     1921 catgtctgag ccgaacgctc accccggtca ccggcgcgtc ggtgcgcgac ccgaacagat
     1981 cagcgcccct cgatccgccc gagcagccgc aggagcccgt cgagaatcgt gtacggatgc
     2041 ggcgggcacc ccagcacgaa cagatcgacc ggcaccgtgg aggccgcccc gttgtgcacc
     2101 tcgggatggt cgatatacgg ccctccattc accgcgcacg ccccgacggc aatcacgatc
     2161 ttcggcggcg gaatcgcgtc gtacgtcttc ttcagcgcga gccgcatgtt ctcggacacc
     2221 gctcccgtga tgagcagccc gtccgcatgc cggggagacg ccacgtactg gatgccgaag
     2281 cggcccaggt cgaacactgg cgtgccgagc acgttcacgt ccgcctcgca ccccgcgcag
     2341 ccgcccgcgc tcacgacccg cagcttcaag gaccgcccga agagccgccg cagcttgtcg
     2401 tccagcgccg ccgcacgcgg atattccttg ccgcgcacga tcaaatcctc gcgcttgcgc
     2461 gccgcaaggc tgtgcccgga cgtgaaggag atggcgccct cggggcacgc atcgacgcag
     2521 tcggggcaga agaggcattt ccccatgtcc agcgagagcc cgccgtccag cgaaatggct
     2581 ccggtgggac atgcctccgc gcaggcctgg cagccgtcgg cgcagcgcga cggctcgagc
     2641 accggcagcc cgcggaagcg atcgggcagc gccggcggct gcagcgggta cgccgcggtc
     2701 cggtgtccct gccgtgcgcg cgcgagcagg atcttcagca tgcgttcttc ctcacaggt
//
LOCUS       DATDMP010000138         2214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2020600, whole genome shotgun sequence.
ACCESSION   DATDMP010000138 DATDMP010000000
VERSION     DATDMP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020600"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VI195_02280"
     CDS             complement(<1..427)
                     /locus_tag="VI195_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823241.1"
                     /translation="MADLEFARARAELAATGSAALVARAELIRCAARAASLEFDDCPG
                     FEKLRADAGTEELAYADFLAGRAPRTATEDVFSRLVSAGVDFKNGKATPQSIAAAIEL
                     SSAQGWRRPLLAWLGVEEKRAEAAGDAAALERVRRRIALV"
     gene            complement(559..1458)
                     /locus_tag="VI195_02285"
     CDS             complement(559..1458)
                     /locus_tag="VI195_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY6823242.1"
                     /translation="MLLGLSIIIAAGFIVYVLYARGVFEQTQRLYLVADNAEGLNVGA
                     DLSFAGFPIGRVRRIRLRDDGKVRISVRVPVDDAKWLRASSVFVLDVPLVGAAKLRAF
                     TADLKDAPLPDRAERPLLRGDTAQEIPRLVASVRSILESLDEMTQAGSSLQQSLSNTR
                     TLTERMAGKQGLLGAALGSEENAKKVIAAIDHTNAVLARLDGVLAKTDQRILSQGGLV
                     DEVQKAVVQANAILGEVRASLKRVDGILADAQAVAGNAKAATADLGALRAEVEASLRK
                     VSALIDEINRKWPFERDTKIKLP"
     gene            complement(1560..>2214)
                     /locus_tag="VI195_02290"
     CDS             complement(1560..>2214)
                     /locus_tag="VI195_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6823243.1"
                     /translation="MAVRQIYFMAWKVLAGYLIFSALLSLVIIEVVSSAARTYGVARY
                     AIELVLRVLVLEVIPLMTALFVALRTGSEVGAEIALMRVSGEIEDSEEITATPLYSEL
                     VPRIAGAALSVFTLMTLSCALAGALTYTVFYGFTDAGFESFSGVVANVFDEMVLVGFV
                     LKCLLFGLAVALIPLATGLEAERGQPRTVPFAVLAGLVKLFFVIGVVAVLSLVVKYF"
BASE COUNT          366 a          790 c          736 g          322 t
ORIGIN      
        1 caacgagcgc gatgcggcgc cggacgcgct ccagcgccgc cgcatcgccc gccgcttcgg
       61 cgcgcttctc ttccacgccc agccacgcca gcagcggcct gcgccagcct tgcgctgagg
      121 acagctcgat cgcggcagcg atggattgcg gggtggcctt gccgttcttg aaatcgactc
      181 ccgcagaaac cagtcgcgaa aaaacgtctt ccgtagcggt gcgcggcgcg cgccccgcga
      241 ggaagtccgc atatgccagc tcttcagttc cggcgtccgc gcgcagcttc tcgaagcccg
      301 ggcagtcgtc gaactcgagg ctcgccgcgc gcgcggcgca gcggatgagc tcggcgcgcg
      361 cgaccagcgc ggcgctgccg gtggccgcga gctcggcgcg cgcgcgcgcg aattccaggt
      421 ccgccacgcg gctgtcgccg cgcaggtaag cctgccggaa ggcgtcgagc gagtatttcg
      481 cgctcgcctg ccatgcaggt tgcgctggcc cgcctgaaca gccaaagaga aaaattccta
      541 gagatagaaa caagaatctc acggcagctt gatcttggtg tcgcgctcga acggccactt
      601 gcggttgatc tcgtcgatca gcgccgagac cttgcgcagg ctcgcctcga cctcggcgcg
      661 cagcgcgccc aggtcggcgg tcgccgcctt ggcgttgccc gccaccgcct gcgcgtcggc
      721 aaggatgccg tccacgcgct tcaggctcgc gcgcacctcg ccgaggatgg cgttcgcctg
      781 caccaccgcc ttctgcacct cgtcgaccag gccgccctgg ctgagaatgc gttggtcggt
      841 cttggcgagc acgccgtcca gcctggcgag caccgcgttg gtgtggtcga tcgcggcgat
      901 caccttcttc gcattctcct ccgagccgag cgccgcgccg agcaggccct gcttgccggc
      961 catgcgctcg gtcagggtgc gcgtgttgct caggctctgc tgcaggctgg agccggcctg
     1021 cgtcatctcg tccaggctct cgaggatgct gcgcaccgag gcgaccaggc gcgggatctc
     1081 ctgcgcggtg tcgccgcgca gcagcggacg ctcggcgcgg tcgggcagcg gcgcgtcctt
     1141 cagatcggcg gtgaaggcgc gcagcttggc cgcccccacg agcggcacgt cgagcacgaa
     1201 gacgctcgag gcgcgcagcc atttcgcgtc gtcgaccggc acgcgcacgc tgatgcgcac
     1261 cttgccgtcg tccctgagcc ggatgcggcg cacgcggccg atgggaaaac cggcgaagct
     1321 caggtccgcg cccacgttca ggccctcggc attgtcggcg acgaggtaca ggcgctgcgt
     1381 ctgctcgaac acgccgcgcg cgtacagcac gtagacgatg aagccggcgg cgatgatgat
     1441 cgacaggccg agcagcatgc cgaccttgac gccgaggctg cgcggaaaga gggagaaacc
     1501 ctctcgcgag aaccctccgc ctgcgcccgt gccgccctgc gtgtccggtt gctcggccat
     1561 cagaagtatt tcaccaccaa tgacagcacc gcgacgacgc cgatcacgaa gaacagcttc
     1621 accaggcccg cgagcactgc gaacggcacc gtgcgcggct ggccacgctc ggcttccagg
     1681 ccggtggcga gcgggatcag cgccaccgcc aggccgaaca gcaggcactt gagcacgaag
     1741 cccaccagga ccatttcgtc gaagacgttg gcgaccacgc ccgagaagct ctcgaagccc
     1801 gcgtcggtga agccgtagaa gaccgtgtag gtgagcgcgc ccgcaagcgc gcaggagagc
     1861 gtcatcagcg tgaacacgga cagcgctgcg ccggcgatgc ggggcaccag ctccgagtaa
     1921 agcggcgtcg ccgtgatctc ctcgctgtcc tcgatctcgc cgctcacgcg catcagcgcg
     1981 atctcggcgc cgacctccga tcccgtgcgc agcgcgacga acagtgcggt catgagcggg
     2041 atcacctcca gcaccagcac gcgcagcacg agctcgatcg cgtagcgcgc gacgccgtag
     2101 gtgcgggccg cgctgctgac gacctcgatg atcaccaggc tgagcagtgc gctgaagatg
     2161 agatagccgg ccagcacctt ccaggccatg aaatagatct ggcgcaccgc catc
//
LOCUS       DATDMP010000139         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_800008, whole genome shotgun sequence.
ACCESSION   DATDMP010000139 DATDMP010000000
VERSION     DATDMP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800008"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VI195_02295"
     CDS             <1..607
                     /locus_tag="VI195_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6823244.1"
                     /translation="IQRRIDPTLAYRYACRVGMCGSCAMTVNGAPRWTCRTHVDKVAA
                     SGELELRPLRNLPVVRDLVADMREFFDKWQRAKGQFSGASSKSDDFARVAPDAPGRRA
                     ADAGIECIGCGVCYAACDVVSWTRDYLGPAALNRAWTLISDVRDVASMDRLRAVAGDA
                     GCHACHTHTSCTERCPKHISPTAGIAGLKRAIAKATVAGKL"
     gene            604..942
                     /locus_tag="VI195_02300"
     CDS             604..942
                     /locus_tag="VI195_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823245.1"
                     /translation="MSTRSDTLFWLAQRASAAVLALCVTVHLVTIIYAVRGGLTAAEI
                     FGRTRGSAGWLAFYSLFVIACAVHAPLGLRPVLTEWLGWRGRSREAFVLAFGVLLALL
                     GMRAVLTVFR"
     gene            939..1238
                     /locus_tag="VI195_02305"
     CDS             939..1238
                     /locus_tag="VI195_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823246.1"
                     /translation="MKHVALIHRLSGIALALFLPVHFWALGRGLELDSFLRWTEQPLV
                     RLAEWGIVVLLATHLGGGLRVLAIEFLDWHEWQKSLAAGAGALAVAVGLAFALAL"
     gene            1260..>2008
                     /locus_tag="VI195_02310"
     CDS             1260..>2008
                     /locus_tag="VI195_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6823247.1"
                     /translation="MRTLTLIFAALLSFAAHAQSWPQKPVRFIVPFAPGGATDIAARM
                     LGEKLSQVWGQSVVVENRAGAGGALGAAEAARAAPDGYTLFFPSGSVMTANQHVYAKL
                     NYDPERDFVPVTNVVSGPQVLVVPANSPYRTVKQLIDDARANPGKLTFGHAGIGTQTH
                     LAAENFLYQAKIDATSVPYKGEAPAIVGLVGGEISFALTNMAAAIQHINGGRLRALGV
                     TSKEVASQLPSVPPIAKTLPGFENAGWFGIVA"
BASE COUNT          295 a          663 c          698 g          352 t
ORIGIN      
        1 catccagcgc cggatcgacc cgacgctcgc ctatcgctac gcctgccgcg tcggcatgtg
       61 cggctcgtgc gccatgacgg tgaacggagc gccgcgctgg acctgccgca cgcacgtcga
      121 caaggtggcc gcgagcggcg agctcgagct gcggccgctg cgcaacctgc ctgtggtgcg
      181 cgatctcgtg gccgacatgc gcgagttctt cgacaagtgg caacgcgcca agggccagtt
      241 ctcgggcgcg agcagcaagt ctgatgactt tgcgcgcgtc gcgcccgacg cgcccgggcg
      301 ccgcgcggcg gacgccggca ttgagtgcat cggctgcggc gtctgctacg ccgcgtgcga
      361 cgtcgtaagc tggactcgtg actatcttgg accggcggcg ctcaatcgcg cctggacgct
      421 gatcagcgat gtacgcgacg tcgccagtat ggatcgcttg cgcgcggtgg ccggcgacgc
      481 cggctgccac gcctgccata cgcataccag ctgcaccgag cgctgcccta aacacatctc
      541 cccgaccgcc ggtatcgctg gtctgaagcg cgcaatcgcc aaggccacgg ttgccggcaa
      601 gctgtgagca cgcgaagcga cacgcttttc tggctcgcgc agcgcgcgag cgctgcggtg
      661 cttgcgttgt gcgtcacggt gcacctcgtg accatcatct atgcggtgcg cggcgggctc
      721 actgcggcgg agattttcgg ccgcacgcga ggtagcgccg gctggctcgc tttctactcg
      781 ttgttcgtca tcgcctgcgc ggtgcacgcg ccgctcggcc tgcggccggt gctgacggaa
      841 tggctcggct ggcgcggccg ctcgcgtgaa gcgttcgtgt tggcattcgg ggtgctgctc
      901 gcgctgctgg gaatgcgcgc tgtgctcacg gtgttccgat gaagcacgtc gcgctcatcc
      961 atcgactctc gggcatcgca ctcgcgcttt tcctgccggt gcatttctgg gcgctcggac
     1021 gaggcctgga gctcgattcg ttcttgcgct ggaccgagca gccgctcgtc agactggccg
     1081 aatggggcat cgtcgtgctg ctcgccacgc acctgggtgg gggcctgcgc gtcctggcga
     1141 tcgagtttct cgactggcat gagtggcaga agagcctcgc cgcaggcgcc ggcgcgctcg
     1201 ccgtggccgt cggcttggcg ttcgcgttag cgctgtagaa ttttccgagg agggtaggaa
     1261 tgagaacgct gacgctgatc ttcgccgcgc tcctgagctt cgcggcgcac gcgcagtcgt
     1321 ggccgcagaa gccggtgcgc ttcatcgtgc cattcgcgcc aggcggcgcg accgacatcg
     1381 cggcgcgcat gctgggcgag aagctctcgc aagtctgggg acagagcgtg gtggtcgaga
     1441 atcgcgccgg cgccggcggc gccctcggtg cggcggaagc ggcgcgggcg gcaccggatg
     1501 gctacacgct cttctttccc tcgggctcgg tgatgaccgc gaaccagcac gtctacgcga
     1561 aactcaacta cgaccccgag cgcgacttcg tgccggtgac caacgtggtc tccggtccgc
     1621 aggtgctggt cgtgccggcg aattcgccct accgcacggt gaagcagctg atcgacgacg
     1681 cgcgtgcaaa tcccggaaag ctgactttcg gccatgcagg catcggcacg cagacgcatc
     1741 tggcggctga gaatttcctc taccaggcga agatcgacgc cacctcggtg ccgtacaagg
     1801 gcgaggcgcc ggcaattgtc ggcctcgtcg gtggcgagat cagcttcgcg ctcaccaaca
     1861 tggcggcggc gatccagcac atcaacggcg ggcggctgcg cgcgctgggc gtgaccagca
     1921 aggaagtggc gagccaattg ccgagtgtgc cacccatcgc gaaaacgctt ccgggattcg
     1981 agaacgccgg ctggttcggc atcgtggc
//
LOCUS       DATDMP010000140         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_244560, whole genome shotgun sequence.
ACCESSION   DATDMP010000140 DATDMP010000000
VERSION     DATDMP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244560"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VI195_02315"
     CDS             <1..1680
                     /locus_tag="VI195_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823248.1"
                     /translation="AFAAMSYLEAPPMLWLAVVALLMLHFAGWRKPVQLTAAIALWLF
                     AVLGTYSLGGIGPGGLKVPAEVFWSPYYKVAYSGPFRAIATNDIGHQQMADIRRHGPA
                     YRLPHALNREAGGVPFERVMIIGAGSGNDVAVALRAGARHVDAVEIDPRINALGRRFH
                     PNQPYADPRVSIHLDDGRSFVRHTEGKYDLAVYALVDSLMLHSGYSSLRLENFLFTHE
                     ALEDVKRTLAPGGVFAMYNYFRQGWVVTRLAAMAEKTFGTKPLVFNLPYSGEINPQDV
                     QRGAWTLILAGDAARLDAIRAKFAEQGSFWLESEANAERALDHFSAAAPDEPALRVAP
                     AKVAASSTQRLPSDDWPHLYLRDASIPWAPIGQGMVTVAVLSIVLLAAFAGRRRLRPN
                     AQMFFLGAGFMLLETKGVVHMALLFGGTWVVNSVVFFAILVMILCANLFVMRVKPRRL
                     APYYALLVVALLVNVLVPMSAFLALPPLERTLASCAVVFVPIFFAGVIFASLFAASRD
                     PDIDFGSNVAGIILGGLSEPLSLVLGFNALIAVAIGYYALSLAARARAMPA"
     gene            1677..>2138
                     /locus_tag="VI195_02320"
     CDS             1677..>2138
                     /locus_tag="VI195_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823249.1"
                     /translation="MIDVSRLLLVVGAIVLVALLTFIPLSSNDFWLHVTIGGMIWQDG
                     AIPKTALFPFTEAKDYPFIAHEWLPSVLFYLLNQWLGYDHLLFVKGALGLALFAVCYR
                     LAHRLTDDTVIAILVALAAMVVANYRHFLRPETLAMLFLVVQLNVLVEYELT"
BASE COUNT          300 a          723 c          695 g          420 t
ORIGIN      
        1 gcgttcgcgg cgatgagcta tctcgaggcg ccgccgatgc tgtggctggc ggtggttgca
       61 ctgcttatgc tgcatttcgc cggatggcga aaaccggtgc agttgaccgc cgccatcgcg
      121 ctgtggctat tcgccgtgct cggcacctat agcctgggcg gcatcggccc cggcggcctg
      181 aaggtgcccg ccgaggtgtt ctggtcgccc tactacaagg tggcttattc gggcccgttc
      241 cgcgctatcg ccacgaacga catcgggcat cagcagatgg ccgacatccg gcgccacggg
      301 ccggcttacc gcctgccgca tgcgctcaac cgggaggcgg ggggcgtgcc tttcgagcga
      361 gtgatgatca tcggcgcggg cagcggcaac gacgtggccg tcgcgctgcg cgcgggcgcg
      421 cggcacgtcg acgcggtgga gatcgacccg cgcatcaacg cgctcggccg acggtttcat
      481 ccgaaccagc cgtatgccga ccctcgcgta tcgattcatc tcgacgatgg gcgcagcttc
      541 gtgaggcata ccgagggcaa atacgatctg gcggtgtacg cgctggtcga ttcgctgatg
      601 ctgcattccg gttattccag cctgcggctg gagaacttcc tcttcacgca cgaggcgctc
      661 gaggacgtca agcgcacgct cgcacccggc ggcgtgttcg ccatgtacaa ctacttccgc
      721 cagggttggg tggtcacgcg gctcgctgcc atggccgaga agaccttcgg taccaagccg
      781 ctggtgttca acctgccgta cagcggcgaa atcaacccgc aggacgtgca gcgcggcgcc
      841 tggaccttga tcctcgccgg cgacgcggcg cggctcgacg cgatacgcgc caagttcgcc
      901 gagcagggga gcttctggct cgaatccgag gccaacgccg aaagagcgct cgatcacttc
      961 agcgccgcag cgcccgacga accggcgctg cgcgtggctc cggcaaaggt ggccgcctcg
     1021 agcacgcagc ggctgccgag cgacgactgg ccgcatctct atttgcgcga cgccagcatt
     1081 ccttgggcac cgatcggcca gggcatggtg accgtagcgg tgctgtccat cgtgctgctc
     1141 gccgccttcg ccggccggcg gcgcttgcgg ccgaacgcgc agatgttctt tctcggcgcg
     1201 ggcttcatgc tgctcgagac gaagggcgtg gtgcacatgg cgctcctctt cggcggcacc
     1261 tgggtagtca actcggtggt gttcttcgcc atcctggtga tgatcctttg cgccaatctc
     1321 ttcgtgatgc gggtgaagcc gcggcgcctc gcgccgtact acgccctgct ggtcgtcgcg
     1381 ctgctcgtca acgtcctcgt gccgatgagc gcgttcctcg cgctgccgcc gctcgaacgc
     1441 acgctcgcct cctgcgccgt ggttttcgtg cccatcttct tcgccggcgt gatcttcgcg
     1501 agcctgttcg ccgcaagccg cgacccggac atcgacttcg gctcaaacgt cgccggcatc
     1561 atcctcggcg gtctgagcga gcccttgtcc ctggtgctcg ggttcaacgc cctgatcgcc
     1621 gtggcgatcg gctactacgc gctgtctttg gcggcgcgcg cgcgcgccat gcccgcgtga
     1681 tcgacgtcag ccggctgctg ctggtcgtcg gggcgatcgt gctggtcgcg ctcctcacct
     1741 tcataccgct cagctcgaac gatttctggc tgcacgtgac gattggcgga atgatctggc
     1801 aggacggggc gattccgaag accgcgctct ttcccttcac cgaggcgaag gactacccgt
     1861 tcatcgccca cgagtggctg ccctcggtgc tgttttacct gctgaaccag tggctcggtt
     1921 acgaccacct gctgttcgtc aagggcgcgc tcgggcttgc cttgttcgcg gtgtgctacc
     1981 ggcttgcgca ccgcctgacc gatgacacgg tgatcgccat cctggtcgcg ctggccgcga
     2041 tggtggtggc gaattaccgg cacttcctgc ggccggaaac cctggcgatg ctcttcctcg
     2101 tcgtgcagct caacgtgctg gtcgaatacg agctcacc
//
LOCUS       DATDMP010000141         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1799931, whole genome shotgun sequence.
ACCESSION   DATDMP010000141 DATDMP010000000
VERSION     DATDMP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799931"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VI195_02325"
     CDS             complement(<1..753)
                     /locus_tag="VI195_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6823250.1"
                     /translation="MSAPAAALEMAGVRKSFGITEIIRGVDLAIPKGERHALIGPNGA
                     GKSTLFNLMSGRFPVTAGAIRLNGEDITRLKPFQINRKGLSRSFQITNIFHNLSVFEN
                     LRCAVLWALGYKYSFWHRLNALTDARQRADEVLHAIGLEARRDRPAGLLTYAEQRALE
                     IGITIAGGADVILLDEPTAGMSRSETAHVVELVRRVTEGKTLLMVEHDMGVVFNLADR
                     ISVLVYGEVIATGTPEEIRANPAVQEAYLGKEA"
     gene            complement(750..2015)
                     /locus_tag="VI195_02330"
     CDS             complement(750..2015)
                     /locus_tag="VI195_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6823251.1"
                     /translation="MRYQPLNLGRWIVWGATALLMASLPLVVTQGFAITLLSQMGIAI
                     IFALSYNMLLGQSGMLSFGHAVYSGLGAYIAVHVLNMASKGQIWMPVSLLPLVGGLAG
                     AFFGVLFGYVTTKRAGTTFAMITLGIGELVFNCSLMFPEFFGGEGGITTNRVFGQPFL
                     GLTFGPAVQVYYLIALWTLASMALMYAWSHTPLGRIANAVRDNPERAEFVGYDTQKVR
                     WLTLIISAFFAGVAGALGCITFEIVTAENVSAARSGGVLLAAFIGGVGFFFGPIIGAC
                     LFVYFVVALSDHTKAWLLYLGLFFMAMVMFAPGGIASLLMMQLPVIARRRFRELVPHY
                     ARTGAAALLVLAALVLTVEMTYKVSVDSANGTAMKMFGVEFDAAGAVPWALAIGLWIV
                     GGWLLQATRRRLADAWGEIQAAIAEEARA"
     gene            complement(2022..2969)
                     /locus_tag="VI195_02335"
     CDS             complement(2022..2969)
                     /locus_tag="VI195_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6823252.1"
                     /translation="MEFFVISLLNGVSYGLLLFMLSSGLTLIFSMMGVLNFAHASFYM
                     IGAYFAYSIGLKVGFFPALVLAPVLVGLVGAIVERYGLRQVHRYGHVPELLFTFGLAY
                     LLEETVKVVWGLPAVPFRVPELLDGHLFTLYSTSFPKLRGFMMLVALAMLASVYLLLT
                     RTRIGLVIQASLSHPQTVEALGHNVPRVFMLVFGGGCALAGLAGVIGGVAFITEPSMA
                     ASVGPIVFVVAVVGGMGSLAGAFLASLLIGVIQTFAIALNWSLAGMLGSLGVTVTAQT
                     PLYSVWKLQLAEVAPVLPYLLLVLMLIFRPKGLLGTRAD"
BASE COUNT          597 a         1076 c         1103 g          426 t
ORIGIN      
        1 ggcctccttg ccgaggtagg cttcctgcac cgcggggttg gcgcggatct cctcgggcgt
       61 gccggtggcg atcacctcgc catagacgag cacgctgatg cggtcggcga ggttgaacac
      121 cacgcccatg tcatgctcga ccatgagcag cgtcttgccc tcggtgacgc ggcgcaccag
      181 ctcgaccacg tgcgcggtct ccgagcggct catgcctgcg gtcggctcgt cgagcaggat
      241 cacgtcggcg ccgccagcga tggtgatgcc gatctccagc gcgcgctgct cggcgtaggt
      301 gagcagcccc gcgggccggt cgcgccgcgc ttccaggccg atcgcgtgca gcacctcgtc
      361 ggcgcgctgg cgcgcgtcgg tgagcgcgtt cagccggtgc cagaacgagt acttgtagcc
      421 gagcgcccac aatacggcgc agcgcaggtt ctcgaacacc gacaggttgt ggaagatgtt
      481 ggtgatctgg aagctgcgcg acaggccctt gcggttgatc tggaagggct tcaggcgcgt
      541 gatgtcctcg ccgttgaggc ggatcgcgcc cgccgtgacg ggaaagcgcc cgctcatcag
      601 gttgaacagc gtcgacttgc ccgcgccgtt cgggccgatg agcgcgtggc gctcgccctt
      661 cgggatcgcg aggtcgacgc cgcggatgat ctcggtgatg ccgaacgact tgcgcacccc
      721 cgccatctcc agcgcggccg ccggggcgct catgcgcgcg cctcctccgc gatggcggcc
      781 tggatttcgc cccaggcatc cgcgaggcga cggcgcgtgg cctggagcag ccagccgccg
      841 acgatccaca gcccgatggc gagcgcccag ggcacggcgc ccgccgcgtc gaactcgacg
      901 ccgaacatct tcatcgcggt gccgttggcg ctgtcgaccg agaccttgta ggtcatctcg
      961 accgtgagca cgagcgcggc cagcaccagc agcgcggcgg cccccgtgcg cgcgtagtgc
     1021 ggcaccagct cgcggaagcg ccgccgcgca atcaccggca gctgcatcat caagaggctc
     1081 gcgatgccgc ccggcgcgaa catcaccatc gccatgaaga acaggccaag gtagagcaac
     1141 caggccttgg tgtggtccga gagcgccacc acgaagtaca cgaacaggca ggcgccgatg
     1201 atcgggccga agaagaaacc caccccgccg atgaaagcgg cgagcagcac gccgccggag
     1261 cgcgccgcgc tcacgttctc ggcggtgacg atctcgaagg tgatgcagcc cagcgcgccc
     1321 gcgacgccgg cgaagaacgc cgagatgatg agcgtcagcc agcgcacctt ctgcgtgtcg
     1381 tagccgacga actcggcgcg ctccgggttg tcgcgcaccg cgttggcgat gcgcccgagc
     1441 ggcgtgtgcg accaggcgta catgagcgcc atcgaggcga gcgtccagag cgcgatcagg
     1501 tagtagacct ggaccgccgg cccgaaggtc aagccgagga agggctgacc gaacacgcgg
     1561 ttggtggtga tgccgccctc gccgccgaag aactccggga acatcagcga gcagttgaac
     1621 accagctcgc cgatccccag cgtgatcatc gcgaaggtgg tgccggcgcg cttggtggtg
     1681 acgtagccga acagcacgcc gaagaaggcg cccgcgagcc cgcccaccag cggcagcagc
     1741 gagaccggca tccagatctg gcccttgctc gccatgttga gcacgtgcac ggcgatgtag
     1801 gcgcccaggc cggagtagac cgcgtggccg aaggacagca tgccgctctg gccgagcagc
     1861 atgttgtagg acagcgcgaa gatgatggcg atgcccatct gcgacagcag cgtgatggcg
     1921 aagccctgcg tcacgaccag gggcagcgac gccatcagca gcgcggtggc gccccagacg
     1981 atccagcggc cgaggttgag aggctggtag cgcatccggc gtcagtcggc ccgcgtgccg
     2041 agcagcccct tgggccggaa gatcagcatc agcaccagca gcaggtaggg cagcaccggc
     2101 gcgacctccg cgagctgcag tttccagacc gagtacagcg gcgtctgcgc cgtcaccgtg
     2161 acgccaagcg agcccagcat gccggcgagc gaccagttga gcgcgatcgc gaaggtctgg
     2221 atgacgccga tcagcagcga ggcgaggaag gcgcccgcga gcgagcccat gccgcccacc
     2281 accgcgacca cgaacacgat cgggccgacc gaggcagcca tcgagggctc ggtgatgaag
     2341 gcgacgccgc cgatcacgcc ggcgaggccg gcgagggcgc agccgccgcc gaacacgagc
     2401 atgaacacgc gcggcacgtt gtggccgagt gcctcgaccg tctgcgggtg cgagagcgag
     2461 gcctggatca ccaggccgat gcgcgtgcgc gtcagcagca ggtacacgga ggcgagcatt
     2521 gcaagcgcca ccagcatcat gaagccgcgc aatttaggga acgaggtcga atacagcgtg
     2581 aacaagtgac cgtcgaggag ttccggcacc cggaagggca ccgcgggcag gccccagacc
     2641 accttcaccg tttcttcgag caggtaggcc aggccgaatg tgaacagcag ttccggcacg
     2701 tggccgtagc gatgcacctg gcgcaggccg tagcgctcga cgatcgcgcc caccaggccg
     2761 accagcaccg gcgccagcac gagcgccggg aagaagccga ccttcaggcc gatcgagtag
     2821 gcaaagtacg cgccgatcat gtagaagctc gcgtgcgcga aattcagcac gcccatcatg
     2881 ctgaaaatga gcgtcagccc ggacgacagc atgaacagca gcagcccgta ggacacgccg
     2941 ttcagcagcg agatgacgaa gaattccacg cgcgtgcgcc gaaaaaaagg ccgggcgcgg
     3001 gcgcccggcc ttcgaggact atcgagtcgt caagccggcg gctacttcgg ccgctgcatc
     3061 tggcaggagg tgggctgcgt cgcgaggtag gtcggcagca gcgcctcggt cttccagccg
     3121 tagccggtgt tctcctggtc gaaacgcacg tccttcttgt tcaccttgac ccacttggcc
     3181 acatacagcg gctgcagggc ct
//
LOCUS       DATDMP010000142         3799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_356196, whole genome shotgun sequence.
ACCESSION   DATDMP010000142 DATDMP010000000
VERSION     DATDMP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356196"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VI195_02340"
     CDS             complement(<1..265)
                     /locus_tag="VI195_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HEY6823253.1"
                     /translation="MSHPVATEIRLRRASRVLEVSFSDGSRFELPFEYLRVHSPSAEV
                     KGHGPGQEVLVLGKEQVGISAVEPVGQYAVKLVFDDGHDTGLYT"
     gene            448..2259
                     /locus_tag="VI195_02345"
     CDS             448..2259
                     /locus_tag="VI195_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6823254.1"
                     /translation="MEPTDTSHPDYYHRVVDCQWACPAHTDVPEYIRLIAQGRFTDAY
                     MVNRHSNVFPGILGRVCDRPCEPACRRGRIEAKPVAICRLKRVAADHREDVSARLPKI
                     PRLKNGKRIACIGAGCASLTVANDLMPLGYEVTIFEQYGEPGGLMRTNIPSFRLPAAV
                     LSEEIAMIVDMGVELKLNSPVQSMRALLAQGFDAVFVGSGAPKGKELRLPGRDEGAAN
                     IHIGIAWLESVAFGHIDKVGKRVLIIGVGNTAMDCCRTSLRLGAESVKVMARKPRGFF
                     KASAWELEDAEEESVQIIVNRSPKAFVIENGRLVGMRFDQMEYELDTHGNIRAERILG
                     EEFLPADDVILAIGQENAFPWIERDLGIEFGKWDVPVVDTTTFQSTLPAVFFGGDAAF
                     GPKNIIWAVEHGHQAAISIHRHCQGEAVHERLPPGMNLQSRKMGMHEWSYANDYTPVE
                     RRLMPHVSLKERFKKLNIEVELGFTAEQVEQEVQRCLNCDMQTAFTAKLCIECDACID
                     ICPVDCLTITHNGTEAELRTRLKAPALNLSQPLYVSAGLPQTGRVMVKDEDLCVHCGL
                     CAERCPTAAWDMQKSELHWPHAADEAGALEARKPKIA"
     gene            2313..>3799
                     /locus_tag="VI195_02350"
     CDS             2313..>3799
                     /locus_tag="VI195_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY6823255.1"
                     /translation="MPKADPPRINDFTIKIATVNGTGSASANTLLMKSIFRSGIPVMG
                     KNYFPSNIQGLPTWYEIRVTKDGYVARSARVDIMVAMNAETYARDVKELAPGGYLVYD
                     STWPRPALLKREDITVLGVPLARLCNENFNGVRTRILMKNICYAGVLAALLDLDLANI
                     RGLLAETYAKKPQLVESNMKAIQLGYDYARTHLQCPLPLHVAKMAHTAGHIMIDGNTA
                     AALGCLFAGATVAAWYPITPSTSLMDAFKMFADRTRTDAKTGRHNVAFIQAEDELAAV
                     GMAIGAMWNGARAFTATSGPGISLMNEFLGLAYYAEVPVVIFDIQRVGPSTGMPTRTQ
                     QGDLLLSAYASHGDTRHVCLYPANPEECFYMAAQAFDLAERLQTPVIVLSDLDIGMND
                     WMCRDLKWDDSYRPDRGKMLGREEVLKLEKFYRFLDKDGDGIGYRTLPGTHPKAAYFT
                     RGSGHTQYGAYTEDAAEYQIVLDRLMRKWATAKRLVPRALIDATA"
BASE COUNT          713 a         1262 c         1207 g          617 t
ORIGIN      
        1 acgtgtacag cccggtgtcg tgaccgtcat cgaacacgag cttcacggcg tactgcccca
       61 ccggctcgac cgcgctgata ccgacctgct ccttgccgag gacgagcacc tcctggcccg
      121 ggccgtggcc tttgacttcg gcggatggcg aatgcacgcg caggtactcg aagggcagct
      181 cgaagcggct accgtcgctg aagctcacct ccagcacgcg cgaggcgcgc cgcaggcgga
      241 tttcggtggc tacggggtgg gacataagct tgcggcagta tagtgcgcga ctggacgcat
      301 acattgctgc ggcgcagtat agggaacggc cggccgtgcg cgagacgacg ttccgcgatg
      361 aacgccgatg acaaaccgcc gcgcgaccct atactcacca tcccttttac cgtctgaggg
      421 cccgagggca cgaagcccga gcactgaatg gaaccgaccg atacctccca tccggactat
      481 taccatcgag ttgtggattg ccagtgggcc tgcccggctc acaccgatgt acccgaatac
      541 atccgcttga tcgcgcaggg ccgcttcacc gatgcctaca tggtgaaccg gcactccaac
      601 gtattcccgg gaatcctcgg gcgcgtctgc gaccggccct gcgagccggc ctgccgccgg
      661 ggacgcatcg aggcgaagcc ggtggcgatc tgccgcctga agcgcgtcgc tgcagaccat
      721 cgtgaggatg taagcgcacg actgccgaag atcccacgct tgaagaacgg caagcgcatc
      781 gcctgcatcg gtgcaggctg cgcttcgctc acggtggcga acgatctcat gccccttggc
      841 tacgaggtca ccatcttcga gcagtacggt gagcccggcg ggctgatgcg caccaatatc
      901 ccctcgttcc gcctgccggc cgcggtgctg agcgaggaga tcgccatgat cgtcgacatg
      961 ggggtcgaac tgaagctcaa cagtccggtg cagagcatgc gcgcgctgct cgcgcagggc
     1021 ttcgacgcag tgttcgtcgg ctcgggtgcg ccgaagggca aggagctgcg gcttcccggt
     1081 cgcgacgaag gcgccgccaa catccacatc ggcatcgcct ggctcgagtc ggtcgctttc
     1141 gggcacatcg acaaggtcgg taaacgagtg ctcatcatcg gggtcggtaa caccgccatg
     1201 gactgctgcc gcaccagcct caggctcgga gccgaaagcg tcaaagtcat ggcgcgtaag
     1261 ccgcgcggct tcttcaaggc gtccgcgtgg gagctcgagg acgccgagga ggagagcgtg
     1321 cagatcatcg tcaaccgctc gcccaaggcg ttcgtcatcg agaacgggcg cctcgtcggc
     1381 atgcgcttcg atcagatgga gtacgagctc gacacccacg gcaacatccg cgccgagcgc
     1441 atcctcgggg aggagttcct gcccgcggat gatgtgatcc tcgccatcgg ccaggagaac
     1501 gctttcccat ggatcgagcg cgatctcggc atcgagttcg gcaaatggga cgtgccggtg
     1561 gtcgatacca ccacttttca gtcgacgctg ccggcggtgt tcttcggcgg cgatgcggcc
     1621 ttcggcccga agaacatcat ctgggcggtc gagcacggcc accaggcggc gatctccatc
     1681 catcgccact gccagggcga ggcggtgcac gagcggctgc cgcctggcat gaacctgcag
     1741 agccgcaaga tgggcatgca cgagtggtcg tacgccaacg actacacgcc ggtcgaacgg
     1801 cgcttgatgc cgcacgtctc gctcaaggag cgcttcaaga aactcaacat cgaggtcgag
     1861 ctcggcttca ccgccgagca ggtggagcag gaagtgcagc gctgcctcaa ctgcgacatg
     1921 cagacggcgt ttaccgccaa gctgtgcatc gagtgcgacg cctgcatcga catctgcccg
     1981 gtcgactgtc tcaccatcac gcacaacggc acggaggcgg agctgcgtac gcgcctcaag
     2041 gcgccggcat tgaatctgag ccagccgctg tacgtgtcgg cgggtctgcc gcagaccggg
     2101 cgggtgatgg tgaaggacga ggacctgtgc gtgcactgcg ggctatgcgc cgagcgctgc
     2161 ccgaccgccg cctgggacat gcagaaatcc gagctgcact ggccgcacgc ggccgatgag
     2221 gccggcgcgc tcgaggcgcg caagccgaag atagcctgac accggccgtg cgccggctgc
     2281 cagcgagcag cgatccaaca gcgaagcaag ccatgccgaa agccgatccg ccacgcatca
     2341 acgatttcac catcaagatc gccacggtga acggcaccgg ctcggcgagc gccaataccc
     2401 tgctgatgaa gtcgatcttc cgcagcggca tcccggtgat gggcaagaat tacttccctt
     2461 ccaatatcca gggactgccg acctggtacg agatccgcgt caccaaggat ggttacgtgg
     2521 cgcgctccgc ccgggtcgac atcatggtgg ccatgaatgc cgagacctac gcgcgcgatg
     2581 tcaaggagct cgcgccgggc ggctatctgg tgtacgactc tacctggccg cggcccgcgc
     2641 tgctcaagcg cgaggacatc accgtgctcg gcgtgccgct cgcgcggctg tgcaacgaga
     2701 acttcaacgg cgtgcgcacg cgcatcctga tgaagaacat ctgttacgcc ggcgtgctgg
     2761 ccgcattgct cgatctggat ctcgccaaca tccgtgggct gctggcggag acctacgcca
     2821 agaaaccgca gctggtcgag agcaacatga aagccatcca gctcggctac gactatgcgc
     2881 gcacgcacct gcagtgtccg ctgccgctgc atgtcgcgaa gatggcgcac accgccggcc
     2941 acatcatgat cgatggcaat accgccgctg cgctcggctg cctgttcgcc ggcgcgacgg
     3001 tagcggcctg gtacccgatc actccgtcca cctcgctgat ggacgcgttc aagatgtttg
     3061 ccgaccgcac gcgcaccgac gccaagaccg gccggcacaa cgtcgccttc atccaggcgg
     3121 aagacgagct ggcggcggtc ggcatggcga tcggcgccat gtggaacggc gcgcgcgcct
     3181 tcaccgccac ctcggggccg ggaatctcgc tcatgaacga gttcctcgga ctcgcctact
     3241 acgccgaagt gccggtcgtg atcttcgaca ttcagcgcgt cggcccgtcc accggcatgc
     3301 cgacccgtac ccagcagggc gatctgctgc tctcggccta cgcctcgcac ggcgacaccc
     3361 gccacgtgtg tctctacccc gcaaacccgg aagaatgctt ctacatggcg gcgcaggcct
     3421 tcgatctcgc cgagcggctg cagacgccgg tgatcgtgtt gtcggacctg gacatcggga
     3481 tgaacgactg gatgtgccgc gacctcaagt gggacgatag ctatcggccc gaccgcggca
     3541 agatgctcgg ccgggaggag gtgctcaagc tcgagaagtt ctatcgcttt ctcgacaagg
     3601 atggcgatgg catcggttac cgcactctcc ccggtaccca cccgaaggca gcctacttca
     3661 cccgcggctc gggccacacc cagtacgggg cctataccga ggatgcggcc gaatatcaga
     3721 tcgtgctcga tcggctgatg agaaagtggg ctaccgccaa gcgcctggtg ccgcgcgcgc
     3781 tgatcgatgc gaccgcaga
//
LOCUS       DATDMP010000143         2556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1133386, whole genome shotgun sequence.
ACCESSION   DATDMP010000143 DATDMP010000000
VERSION     DATDMP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133386"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VI195_02355"
     CDS             <1..430
                     /locus_tag="VI195_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823256.1"
                     /translation="VVRRVEGGFVASELTSSYKPFGVDLEEWYGPDVVADFHTHICSI
                     HNAPFANFFSLADALANQGLHTVGYMLSLCDRNIRRYDPSQDDRDDEEVDFRSGRVIY
                     LTCGHIVGWVAPLPGDPIRFEVVAERLREPMADGVPRDSL"
     gene            427..780
                     /locus_tag="VI195_02360"
     CDS             427..780
                     /locus_tag="VI195_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823257.1"
                     /translation="MTDLNKAISQALMLATRLRTMSERTKDSPFKGIVAELLLEIAEV
                     QIRIDELVSEHAALKARLQAQSAPQVERCPRCHELGWKVTGRRPVKQPGQPPRIAQAY
                     ACSKCGLKEEVLTAQ"
     gene            847..1650
                     /locus_tag="VI195_02365"
     CDS             847..1650
                     /locus_tag="VI195_02365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012517611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY6823258.1"
                     /translation="MLATLSSNQALIRLSAFAGVLLLMMLWELLAPRRLQVISRARRW
                     PGNLGIVVLDALLLRLVFPTGAAAAALLAEARGWGLFHAVAAPGWLAVVASVILLDLG
                     IYLQHVLFHAVPALWRLHRMHHADLEFDVTTGVRFHPLEVLLSMAIKFGIVAALGSPA
                     VSVLLFEVLLNATSLFNHGNVRLPQRLDRGLRWLVVTPDMHRVHHSVVRRETDSNFGF
                     NLPWWDRLAGTYRAQPQAGHDAMIIGLEQFRDPAELRLDRMLLQPLRNP"
     gene            1783..>2556
                     /locus_tag="VI195_02370"
     CDS             1783..>2556
                     /locus_tag="VI195_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6823259.1"
                     /translation="MPKRKGLRVLALAAVAVPFLDGCVSHGLSSGGTAATAPPRITVL
                     YDAFGRDAAMSKDWGYAALIEYRGKRILFDTGNDPAILEHNARARGADLSRLDFVVMS
                     HRHGDHMGGMSYLLRVNPRVRIYAPKENFGVYGFSLPGSFYRQDSSLPAEQRYYDGSP
                     PAIMKFGSAWPQANIELIENSTEVAPGFHLVALVSDKPTTLELRELSLVIDTPDGAVV
                     VVGCSHPGIERIAAAAAAITPHIQLLMGGLHLVLAKDADI"
BASE COUNT          396 a          841 c          863 g          456 t
ORIGIN      
        1 cgtggtgcgc agggtcgagg gcggcttcgt ggcctccgaa ttgaccagtt catacaagcc
       61 cttcggtgtc gatctcgagg agtggtacgg cccggatgta gtggctgatt tccacaccca
      121 catctgctcg atccacaacg cgccattcgc caatttcttc tccctggccg atgcgctcgc
      181 taaccagggg ttgcacacgg tcggctatat gctgagcctg tgcgaccgca acatccgccg
      241 ctacgatcca tcgcaggacg accgtgacga cgaggaagtc gatttccgca gcggccgcgt
      301 gatatacctc acctgcggcc atatcgtcgg ctgggtggcg ccgctgcccg gcgacccgat
      361 cagattcgag gtcgtggcgg aacgactgcg cgagccgatg gctgacgggg tgccacgcga
      421 ctccttatga ccgatctgaa taaggcgatt tctcaggcat tgatgctcgc aacccggctg
      481 cggacgatga gcgagcggac caaggattcg cctttcaagg gtattgtcgc cgaactgctg
      541 ctcgagattg ccgaggtaca aatcaggatc gacgagctcg tcagcgagca cgccgcactg
      601 aaggcccggc tccaggcgca gtcggctccg caggtcgaac ggtgcccgcg ctgccacgag
      661 ctcggctgga aggtcaccgg gagacggccg gtcaagcaac ccggccagcc gccccgtatc
      721 gctcaggcct acgcctgctc gaagtgcgga ctcaaggaag aggtgctgac ggcgcaatga
      781 gcagccgggg aacgcgctcg cgccgggcgt tgccggttcc ggtccagaag cgataattgc
      841 cggcgagtgc tggcaacgct atcgagtaac caggccctga tccgtctgag cgccttcgcc
      901 ggggtgctct tgctgatgat gctgtgggag ttgctggcgc cccggcggct gcaggtgatc
      961 agccgcgccc gccgctggcc cggcaatctc ggcatcgtgg tgctggatgc cctgctgctg
     1021 cggctcgtct tcccgacggg cgcggccgcc gccgcactgc tcgccgaggc gcgcggctgg
     1081 ggtctgttcc acgccgtcgc cgcacccggg tggctcgcgg tcgtcgcatc ggtgattctc
     1141 ctcgacctcg gcatctacct gcagcacgtc ctgttccacg ccgtgccggc gctgtggcgg
     1201 ctgcaccgca tgcaccacgc cgatctggag ttcgacgtga cgaccggggt tcgcttccac
     1261 ccgctcgagg tcctgttgtc catggcgatc aagttcggca tcgtggccgc cctgggctca
     1321 ccggcggtga gcgtgctgct tttcgaggtg ctgctcaacg cgacatcgct gttcaatcac
     1381 ggcaacgtac ggctgccgca gcggctcgat cggggattgc gctggctggt cgtcacgccg
     1441 gacatgcatc gcgtgcacca ctcggtcgtg cggcgcgaga ccgacagcaa ttttggcttc
     1501 aatctgccgt ggtgggaccg gctcgccggc acttaccgcg cgcagccgca ggccggacac
     1561 gatgccatga tcatcgggct cgagcagttc cgcgatccgg ccgagctgcg gctcgatcgc
     1621 atgctgctgc agccgctccg caatccctga gctccctggg tgccgcgctg gtggcgcgtg
     1681 cggcgcagcg cggtgtagca tcgtgcggcc cgacaggggg agtcgggcgt cacggtgagc
     1741 gccgtcaggc gaagacagcc ggcgcgctca gcagggggcg tcatgccgaa gcgcaagggc
     1801 ctgagggtgc tagctctggc ggcagtcgcc gtgccgtttc tcgacgggtg cgtgagccac
     1861 ggcctgtcgt cgggcgggac tgcggcgact gcgccgccgc gcatcaccgt tctctacgat
     1921 gccttcggcc gcgacgctgc catgagcaag gactggggct acgcggcgct gatcgaatac
     1981 cgcggcaaac gcatactgtt cgacaccggg aatgaccccg cgatcctcga acacaatgcc
     2041 cgggcgaggg gcgctgacct gtcccggctc gatttcgtgg tgatgtccca tcgccacggg
     2101 gatcacatgg ggggcatgag ctatctgctg cgggtcaacc cccgggtgag gatctacgcg
     2161 ccgaaagaga atttcggcgt ctacgggttc tcgctgcccg gcagcttcta ccgccaggac
     2221 tcgtcgctgc cggcggaaca gcgctactac gacggttcgc cgccggcgat catgaagttc
     2281 ggcagtgcct ggccgcaggc caacatcgag ctgatcgaga actccaccga ggtcgcgccc
     2341 ggatttcacc tggtggcgct ggtgtccgac aagccgacca cgctcgagct gcgtgagctg
     2401 tcgctggtga tcgacacgcc ggacggcgcg gtcgttgtcg tcggctgctc gcaccccggg
     2461 atcgagcgga ttgcggccgc agccgcggcc atcaccccgc acattcagtt gctgatgggc
     2521 gggctgcatc tggtgttggc caaggacgcc gatatc
//
LOCUS       DATDMP010000144         5643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1022512, whole genome shotgun sequence.
ACCESSION   DATDMP010000144 DATDMP010000000
VERSION     DATDMP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022512"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VI195_02375"
     CDS             <1..714
                     /locus_tag="VI195_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6823260.1"
                     /translation="IYAVRVGSLANAYSVFLSAPLIVAALSVPLLGERVDGRNWLAIL
                     AGLAGVLIMLRPSASGLSSLGALAALVGATAYALSAIAVRVLTRSDTTVSVVFWTVGL
                     MTVFASAIAAPAWVPIEHEHWKWLAGLGVLAAVAQYLLIEAFRSAPPSVVTPFEYTAL
                     LWGVAIDRAVWHVLPSARVCFGGGIVIVSGLYLIWHQRRQSALESAREPWQPAAEKGR
                     MLRPSSVAVVARKQEEDAP"
     gene            711..1901
                     /locus_tag="VI195_02380"
     CDS             711..1901
                     /locus_tag="VI195_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6823261.1"
                     /translation="MSNAEVLGRFVWHELLTNDTAGAAAFYPKVLPWRTAPSSMPGYT
                     IWMAGQTQIGGLMALPAEAGSTPPHWLIYVGTPNVDSTCSQAQGLGARIVKPPGDIPN
                     VGRFAVLSDPQGATFAVFTPGSGPPPGSPPPQGGFSWHELATTDVAAALRFYGQLFGW
                     TKGPAHEMGAMGVYQIFQHAGAQSGGMCNVQGPSTAPSWLSYVHVSDSGRAVAAAKSA
                     GGRLLHGPMEVPGGSWVAMMMDPQGGTFAVHEEPRTAAQKPAAPRAAAAAKPAAPPKA
                     AAPPKPAAAPAKPAASASPAAATVAKAVVGARVAKRAKKAARKAGRKVRRAMRKTARK
                     TARKVRRTGRKVARRAARPAGRRAMRKAARKTARAVRRGGRRMARRARVVRRRVRRAA
                     RRRR"
     gene            complement(1964..2932)
                     /locus_tag="VI195_02385"
     CDS             complement(1964..2932)
                     /locus_tag="VI195_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY6823262.1"
                     /translation="MRNLQVKIKRPPSRPADLELVETERPTLTNGSFLARALWLAVDP
                     FRCATDHTNPGLPRPGDVVPARGVGEVIESRHDVFNVGSLIVLDCGLQQLCVSEGLHA
                     RLLHPGQTPASTALGVLGAPGMAAYFGLLHLARLRAAETVLVSAAANAAGSMAGQIAM
                     LKGARAIGIAGTREKCDWVTRHARLSACINYAAENLQARLRQLAPNGVDIYFDNVGGE
                     LLEAVVSSGMLARGARVVQNSVSPADPDALLERGGYAPTALQMLRVDLRDFEHRRGEF
                     LREAIAWYGAGRIATKDHVVEGLANAPAHLLLAMQGGNFGRPLVRL"
     gene            3120..3869
                     /locus_tag="VI195_02390"
     CDS             3120..3869
                     /locus_tag="VI195_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6823263.1"
                     /translation="MPRGTPTQLPVPAARARRGYFECRYGQLHVHHAMPPGGGFEEGT
                     PLLCVHDLPGSGRVFIRLLPLAGEDRSVYAPDLPGFGESDAPSARPTMSEYAAALGDF
                     IDNMRLRNLAVLGLRGGAVPAIELALTRPAQVSRVMLVSVPLLTEAERQAARTQIQPA
                     AAAEGAFRPVEAARWVLEAAAQYPLRERLARLSQRLLVLRPRDDLWEATARVREVLPA
                     TRLAELQQSGPEALAQAPQRIAEAAREFLRA"
     gene            3975..>5643
                     /locus_tag="VI195_02395"
     CDS             3975..>5643
                     /locus_tag="VI195_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823264.1"
                     /translation="MSSRPRPPVPSRAEPDLDTTAELPVLDPACAPDANATLTVQVAV
                     APADEHHAVTDTWAVPAPARAALGAAAGAGAARRAEEAQHDELKAALQDAQERLSSHS
                     KRLVQLERARDEALSAQAAAAQRAASAEQRAASAEQRADHADQRANHAEQRAKHAEQR
                     AEHAGQRSGAFEQRIAALEQHAARLSAELAERQAFAAEQVARLDESARALAAAQKQAT
                     ASGEELAQVRALLNAGGARADQLQQLLEQQDRDTRARHTQELEQQQAQVAEQRTHAAA
                     VIRDLHNERARAMSYLESLQTLERRRELAQQMVTDQQHEAEAAAAELLRLTRAVAAQA
                     ERGRVQDTELAQRAAHIAQLEEQAATLNSLLMHRETELREAQQAADGLRSSVARLQTE
                     INTGAERLRALEAHAAQRMHGDAQQQQELQGLRSQAASLTSALAEARHSAESASARAS
                     SQQAGLAQQRERAAQLEASLVAERQQSRQLEAELTTLRAEMEEWGGVLRSTQQERSGH
                     LASIAAAEARVRELEQHAASRAENVRSLQAECAAHLARVRELESGLHA"
BASE COUNT          899 a         1964 c         2019 g          761 t
ORIGIN      
        1 atctacgcgg tgcgcgtggg ctcactcgcc aatgcgtact cggtattcct gtcggcgccg
       61 ctcatcgtcg ccgccctgtc ggtgccgctc ctcggcgaac gggtcgacgg gcgcaactgg
      121 ctggcgatcc tggccggtct cgcgggggta ctgatcatgc tgcgcccgag cgcctcgggc
      181 ctcagcagcc tcggcgcact cgccgccctg gtcggcgcca ccgcctatgc cctgagcgcc
      241 atcgccgtgc gcgtgctgac gcgcagcgac acgaccgtga gcgtggtgtt ctggaccgtc
      301 ggactgatga ccgtgttcgc gagcgccatt gccgccccgg cctgggtgcc catcgagcac
      361 gagcactgga aatggcttgc agggcttggc gtgctggctg ccgtcgcaca atacctgctc
      421 atcgaggcgt tccgctctgc acctccctcg gtcgtcaccc cgttcgagta cacggcgctg
      481 ctgtggggcg tcgccatcga ccgcgccgtg tggcacgtcc tgccctcggc gcgcgtctgt
      541 ttcggtggcg gcatcgtgat cgtcagcggg ttgtatctca tctggcacca gcgccggcag
      601 tcggccttgg aatccgcgcg agagccgtgg cagccggcgg cggagaaggg cagaatgttg
      661 cgcccatcgt cggtcgcagt agtcgctcgc aaacaagagg aagacgcacc atgagcaacg
      721 ccgaagtcct gggacgattc gtctggcacg agttgttgac gaatgacacc gcgggagccg
      781 ccgcgttcta cccaaaggtt ctgccctggc ggacggcgcc ctccagcatg cccggctata
      841 ccatctggat ggccggacag acgcagatcg gcggcctcat ggcgctgccg gcggaagccg
      901 gctcgacgcc cccgcactgg ttgatctacg tcggcacgcc gaatgtcgac agcacctgct
      961 cccaggcgca ggggctcggc gcgcgcatcg tcaagccgcc gggcgacatc cccaacgtcg
     1021 gacgtttcgc cgtgctgtcg gatccgcagg gtgcgacctt cgccgtgttt acacccggaa
     1081 gcgggccgcc gcccggctcg cctcccccac agggcggctt ctcgtggcac gagctggcga
     1141 ccaccgacgt cgcagccgcg ctgcgcttct acgggcagct gttcggctgg acgaagggtc
     1201 cggctcacga aatgggagcc atgggcgttt atcagatatt ccagcacgcc ggcgctcagt
     1261 ccggaggcat gtgcaacgtg cagggtccct ccactgcgcc ctcctggctg agctacgtgc
     1321 acgtcagtga ctccggtcgc gcggtcgccg ccgccaagag cgcgggcggg agactcctgc
     1381 acgggcccat ggaggtcccc ggtggaagct gggtcgcgat gatgatggac ccgcagggcg
     1441 ggaccttcgc cgttcacgaa gagccgcgca cggcggccca gaagccggcc gcgcccaggg
     1501 ccgctgcagc cgccaagccc gcagcgccgc cgaaagcagc ggcaccgccg aaaccggccg
     1561 cggcgccggc caaaccggct gcgtcggcat cgcccgcggc cgccacggta gcgaaggccg
     1621 tcgtcggtgc gagagtggcc aagcgagcca agaaagcagc tagaaaggca ggtagaaagg
     1681 tgaggagagc aatgagaaag accgcaagaa agaccgcaag gaaggtccgc aggaccggaa
     1741 gaaaggtagc ccggcgcgca gcgagaccgg caggtcgccg ggccatgcgc aaggcggcac
     1801 gcaagaccgc ccgcgcggtg cgccgcggtg gacgccggat ggcacgccgt gcgcgagtcg
     1861 tgcgacggcg ggtacgacgg gccgcgcgcc gcaggcgctg agcctctcag cccgggctca
     1921 ccgcgcgcac tggctgtgtg cgcggtgagc ggtcattcgc tcctcagagc cgcaccagcg
     1981 gccggccgaa gtttccaccc tgcatcgcca gcagcaggtg ggcgggcgca ttggcgagtc
     2041 cttccaccac atgatccttg gtggcgatgc ggccggcgcc gtaccaggcg atcgcctcgc
     2101 gcagaaactc accgcgccgg tgctcgaagt ccctgagatc cacgcgcagc atctggagcg
     2161 ctgttggcgc gtagccgcca cgctcgagca gtgcatcggg atcggccggg gagacgctgt
     2221 tctgtacgac gcgtgcgccg cgcgccagca tgccgctgct cacgaccgcc tcgagtagct
     2281 ccccacccac gttgtcgaag tagatgtcga ccccgttcgg cgccagctgc cgcagtcgcg
     2341 cctgcagatt ttcggcggca tagttgatgc aagccgagag ccgcgcgtga cgcgtgaccc
     2401 agtcgcattt ctcgcgggtg ccggcgatac cgatcgcgcg cgcccccttg agcatcgcga
     2461 tctggccggc catcgagccg gcggcgttgg cggcggcgga gaccagcacg gtctccgccg
     2521 cgcgcaaccg cgccaggtgc agcagcccga agtaggccgc catgccgggc gcgccgagca
     2581 ctccgagcgc cgtcgaggcc ggcgtctgtc ccggatgcaa tagccgggca tgcagtccct
     2641 cggagacaca cagctgctgc agtccgcaat cgagcacgat cagactgccg acattgaaga
     2701 catcgtggcg cgactcgatg acctcaccca cgccgcgggc ggggacgaca tcgcccggtc
     2761 gcggcaaacc tgggttcgtg tggtcggtcg cgcagcgaaa gggatcgacg gccagccaca
     2821 gcgcgcgcgc gagaaaggag ccgttggtga gcgtcgggcg ctcggtctcg accagctcga
     2881 gatcagccgg tcgcgaaggg ggacgcttga tcttcacctg gagattgcgc accgctattg
     2941 tccatccaac tgcgcgcgcg cccgcaccgc ctgctgcgct accgattccg ctgcgcaggc
     3001 ggcggccgtt ccgagcggcg ccgacggcgc ccgcggtaac caggtgcgtc ccatgtcttc
     3061 aatttacccg cggcgaccgc cggcgcaaga ggagtgccga tcgtggcact atgttgcgca
     3121 tgccgagggg aacgccgacc cagctgccag tgcccgcggc gcgtgcacgc cgtggctact
     3181 tcgagtgccg ctacgggcag ctgcatgtgc accacgcgat gccgcccgga ggcgggttcg
     3241 aggagggaac tccgctgctc tgtgtgcacg atctgcccgg ctcaggccgg gttttcatcc
     3301 gcttgcttcc gctcgccggc gaggaccgct cggtgtatgc gcccgacctc cccggattcg
     3361 gcgagtccga cgcccccagc gcccggccga ccatgagtga gtacgccgcg gcgctcgggg
     3421 acttcatcga caacatgcga ctgcgcaacc tcgcggtgtt gggtctgcgc gggggtgccg
     3481 tgccggcgat tgagctcgcg ctcacgcggc cggcgcaggt gagccgggtc atgctcgtat
     3541 cggtgccgct cctgacggag gccgaacgcc aggcggctcg cactcagata cagcctgcgg
     3601 cggcggccga gggcgcgttc cgcccggtcg aggcggcgcg ctgggtgctc gaggccgcgg
     3661 cacagtatcc gctgcgggag cgcctcgcgc ggctcagcca gcggctgctg gtgctgcgcc
     3721 cgcgcgatga cctgtgggaa gcgacggcgc gcgtgcgcga ggtactgccg gccacgcggc
     3781 tcgccgagct gcagcagagc gggcccgaag cactggcgca ggcgccgcag cgcatcgccg
     3841 aagcggcacg cgagttcctt cgggcctgaa aaggggcttt ttcgggagct ttctcacata
     3901 atttgccgga gtgcgtgcgg tgcccacata gtggagcgct acactttttc atcgatgcgc
     3961 atggaagaat ctcgatgagc tcgcgcccgc ggccgccagt gccgtcacgc gcggaacccg
     4021 acctcgacac cacagccgag ctgccggtgc tggaccccgc gtgcgcgccc gatgcgaacg
     4081 ccaccctgac cgtgcaagtc gccgtagccc ctgccgatga gcatcatgcc gtcaccgata
     4141 cctgggctgt gccggctcca gcgcgcgcgg ccctgggtgc cgctgccggc gccggcgcag
     4201 cgcgacgcgc ggaggaagcc cagcacgatg agctcaaagc ggcgttgcag gacgcgcagg
     4261 agcggctctc gagccacagc aagcgcctgg tgcagctcga gcgggcgcgc gatgaggcac
     4321 tctcggcgca ggcggccgcc gcacagcgcg cggcgagcgc cgagcagcgg gccgcgagcg
     4381 ccgagcagcg cgcggaccat gccgatcagc gtgcgaatca tgccgagcag cgcgccaagc
     4441 atgcggaaca gcgtgcggag catgccggac aacgcagcgg cgccttcgag caacgcatcg
     4501 ccgcgctcga gcagcacgcg gcgcggctga gcgcggagct cgcggaacgg caggcctttg
     4561 ctgccgagca ggtggcacgg ctggacgagt cggcgcgtgc cctggctgcg gcgcagaagc
     4621 aagccactgc cagcggtgag gagttggcgc aggtgcgcgc gctgttgaac gccggcggtg
     4681 cgcgcgccga tcagttgcag cagctgctcg agcagcagga ccgcgatact cgcgcgcggc
     4741 acacgcagga gctcgagcag cagcaggcgc aggtggccga gcagcgcacg catgccgccg
     4801 ccgtcataag ggacctgcat aacgagcgcg cccgcgccat gagctatctc gagtcgctgc
     4861 agacgctcga gcggcgccgc gagctcgcgc agcagatggt gacggaccag cagcatgagg
     4921 ccgaagccgc tgcggcggag ctcctgcgcc tcacgcgcgc agtggcggcg caggccgagc
     4981 gcgggcgtgt gcaggatacc gagctggcgc agcgtgccgc acatatcgcg cagctcgagg
     5041 agcaggcggc cacgctcaac agcctgctca tgcaccggga gacggagctg cgcgaagcgc
     5101 agcaggccgc cgacggtttg cgcagcagcg tcgcgcgcct gcagaccgag atcaacacgg
     5161 gtgccgagcg gctgcgcgcg ctcgaggcgc atgccgcgca gcgcatgcac ggcgacgcac
     5221 agcagcagca ggagctgcag ggactgcgct cgcaggcggc gagcctaacg agcgccctgg
     5281 cggaggcgcg ccacagtgcc gagagtgcga gcgcgcgggc gagctcccag caggccggac
     5341 tcgcccaaca gcgcgagcgc gctgcacagc tcgaagcctc cctcgttgcc gagcggcaac
     5401 aaagtcggca gctcgaggcc gagctgacca cgctgcgtgc tgagatggag gagtggggcg
     5461 gggtgctgcg ctcgacacag caggagcgca gcggccatct cgccagcatc gctgcggccg
     5521 aggcgcgcgt gcgcgagctg gagcagcacg ccgcgagccg cgccgagaac gtacggagtc
     5581 tgcaggccga gtgcgccgcg cacctcgcgc gcgtacgcga gctcgagagc ggcctacacg
     5641 ccg
//
LOCUS       DATDMP010000145         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_245635, whole genome shotgun sequence.
ACCESSION   DATDMP010000145 DATDMP010000000
VERSION     DATDMP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245635"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..609)
                     /locus_tag="VI195_02400"
     CDS             complement(169..609)
                     /locus_tag="VI195_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEY6823265.1"
                     /translation="MMTNPVAKITQGQFSFLPELTDAEISLQIEYGLRRGYAWSVEYT
                     DDPHPRNTYWEMFGMPMFDLRDAAGVMLELQSCRKTFPQHYIRLVAFDASRGVESIAM
                     SFIVQRPAREPGFGLIRQEGAGRTVRYTVHGYGTDRPEGERSGG"
     gene            complement(639..2129)
                     /locus_tag="VI195_02405"
     CDS             complement(639..2129)
                     /locus_tag="VI195_02405"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HEY6823266.1"
                     /translation="MMATAIETLGGVGPSAIRDTARRYKAGVLKYRQMGYWEPDYQPK
                     PTDIACLFRITPQEGVDPVEAAAAVAGESSTATWTVVWTDRLTACDNYRAKAFRVEPV
                     PNAPGQFFAWVAYDLILFEEGSIANMTASLIGNVFSFKPLKAVRLEDIHIPPAYVKTF
                     KGPPTGLIVERERLDKFGRPLLGATTKPKLGLSGRNYGRVIYEGLKGGLDFMKDDENI
                     NSQPFMHWRDRFLYVMDAVNKASAATGEVKGSYLNVTAATMEDMYERAEFARQLGSVV
                     VMIDLVVGWTAIQSMSNWARRNDMILHMHRAGHGTYTRQKSHGVSFRVIAKWLRLAGV
                     DHLHAGTAVGKLEGDPLTVQGYYNVCRDSFTRQDLTRGIFFDQDWCDIRKVMPVASGG
                     IHAGQMHQLLDLFGDDVVLQFGGGTIGHPAGIQAGAVANRVALEAMVKARNEGEDIRS
                     KGPEILRKTARFCAPLQQALDTWGEITFNYTPTDACDFTPSAAVAH"
     gene            2222..3205
                     /locus_tag="VI195_02410"
     CDS             2222..3205
                     /locus_tag="VI195_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6823267.1"
                     /translation="MKPATLKNATLKQLRVFSAVARHLSFVRAAEELGLTAPAVSMQI
                     KELETEVGLPLFDRSSRKVSLTMVGEYVLAYTQRVLAAMRDAEDMVARFRGLKTGVLD
                     VAMVSTAKYFLPRLLARFRDEHPGVEVRLEVGNNREEVVALLREGRVELAIMGRPPKN
                     WPSRSEPFAMHPHGLVTSVEHPFARMEKVAARSLMTEGFIVREPGSGTRAALDEYMEA
                     HQMTPRIVMQMSSNEAIKQAVMAGMGVALLSMHTVGLELDHQLIAAPETEGLPVMRRW
                     HVVNTLAKTLSPAAEAFRYFVLETGEAFLARHFGHGSDPTQFGEWHHRRRL"
BASE COUNT          555 a         1078 c         1152 g          626 t
ORIGIN      
        1 catcgatcac ctcgcccgcc tggctcaccg ccagtacctc gctcaccgtg cgggcagtct
       61 cggcagcgcc ggaggcggcg actgcggagg catcggtcgc ggaggcgcct ggcgtggcgg
      121 cgccgggtgc gctgccggga atcgagtaaa gcggtgtgcc caagttcctc accctccgct
      181 gcgctctcct tcggggcggt ccgtgccgta gccgtgcacc gtgtagcgca ccgtgcgacc
      241 tgcgccttcc tggcggatga gcccgaagcc cggctcgcgc gccgggcgct ggacgatgaa
      301 gctcatggcg atgctttcca cgccgcggct ggcatcgaag gccaccaggc ggatgtagtg
      361 ctgcgggaag gtcttgcggc agctctgcag ctcgagcatc acgcccgcgg catcgcgcag
      421 atcgaacatc ggcatgccga acatttccca gtaggtattg cgcgggtgtg gatcatcggt
      481 gtactcgacg ctccaggcgt agccgcggcg cagcccatat tcgatctgca gggagatctc
      541 tgcatccgtc agctccggga ggaagctgaa ctgaccttga gtgatcttcg ccacgggatt
      601 ggtcatcatc gctgcgtgct ccctcgggga ttgcggtctc agtgcgccac cgccgcgctc
      661 ggggtgaaat cgcaggcatc ggtgggcgtg tagttgaagg tgatctcgcc ccaggtatcg
      721 agcgcctgct gcaggggcgc acagaaacgc gccgtcttgc gcaggatctc cgggcccttg
      781 ctgcggatgt cctcgccttc gttgcgtgcc ttgaccatcg cctcgagggc cacgcggttg
      841 gcaactgcgc ctgcctggat gcccgccggg tgaccgatgg tgccaccacc aaactgcaac
      901 accacgtcat cgccgaacag atcgagcagc tggtgcatct gcccggcgtg aatcccgccg
      961 gaggcgaccg gcatcacctt gcggatgtcg caccagtcct gatcgaagaa aatgccgcgc
     1021 gtcaggtcct ggcgggtgaa gctgtcgcgg cagacgttgt agtagccctg cacggtgagc
     1081 gggtcgccct cgagcttgcc cacggcggtg cccgcgtgca ggtgatcgac accggcgagt
     1141 cgcagccact tggcgatcac gcggaaactg acgccgtggc ttttctgccg ggtgtaggtg
     1201 ccgtggccgg cacggtgcat gtgcaggatc atgtcgttac ggcgcgccca gttggacatg
     1261 ctttggatcg cagtccagcc gaccaccaga tcgatcatga cgaccaccga accgagctgc
     1321 ctggcgaact cggcacgctc gtacatgtcc tccatggtcg cggcggtcac gttgagataa
     1381 gagcctttga cctccccggt ggcggcgctc gccttgttga cggcgtccat cacgtagagg
     1441 aagcggtcgc gccagtgcat gaagggctgg gagttgatgt tctcgtcgtc cttcatgaaa
     1501 tcgagtccgc ccttgaggcc ctcgtagatg acacggccgt agttgcggcc cgatagcccg
     1561 agcttcggtt tggtggtggc gccgagcagc ggacggccga atttatcgag gcgctcgcgc
     1621 tcgacgatca ggccggtcgg cggacccttg aaggttttga cgtaggccgg tgggatgtga
     1681 atgtcttcca ggcgcaccgc cttgaggggc ttgaagctga agacattgcc gatgaggctc
     1741 gccgtcatgt tcgcgatcga gccctcctca aacaggatga ggtcgtacgc cacccaggca
     1801 aagaactgcc ccggcgcatt cggtaccggc tcgacccgga aggccttggc gcggtagttg
     1861 tcgcaggcgg tgagacggtc ggtccagacg accgtccacg tcgcggtgct cgactcaccg
     1921 gcgaccgccg ccgccgcctc gaccggatcg accccctcct gaggcgtgat ccggaagaga
     1981 caggcgatat cggtcggctt gggctggtag tccggctccc agtaacccat ctgtcggtac
     2041 ttgagcacgc cggccttgta gcggcgcgcc gtatccctga tcgccgaggg gccgacgcca
     2101 cccaatgtct cgattgctgt tgccatcatc tccccctgcc ggtgacttgt gagccactct
     2161 agaggccgtc tattattcag gtaaagtcac ttttaattag ctaaccgtta agaagtcctt
     2221 aatgaagccc gccacgctca agaacgcgac gctcaagcag ctgcgggtct tctcggcggt
     2281 tgcgcgtcac ctgagcttcg tacgggcggc ggaggagctg ggactcacgg caccggcggt
     2341 gtccatgcag atcaaggagc tggaaacgga ggtcgggctg ccgctgttcg atcggtcgag
     2401 ccgcaaagtg tcgctcacga tggtcggcga gtacgtgctc gcctatacgc agcgggtact
     2461 cgcagccatg cgcgatgcgg aggacatggt ggcccgcttc cgcgggttga agaccggcgt
     2521 gctcgatgtc gccatggtga gcaccgcgaa gtacttcctg ccgcgcctgc tggcgcgctt
     2581 ccgcgatgag cacccgggcg tcgaggtgcg gctggaagtc ggcaacaacc gcgaggaggt
     2641 ggtagcgctg ttgcgcgagg gtcgcgtgga gctcgccatc atgggcaggc caccgaagaa
     2701 ctggccgtcg cgctcagagc ccttcgcgat gcacccccat gggctggtca cctcggtcga
     2761 gcacccgttc gcgcgcatgg agaaagtagc cgcgcgatcg ctcatgacgg aggggttcat
     2821 cgtgcgcgag cccggatcgg gaacgcgcgc cgcgctcgat gagtacatgg aggcgcacca
     2881 gatgaccccc aggatcgtga tgcagatgtc gagcaacgag gccatcaaac aggcagtgat
     2941 ggcgggcatg ggtgtggcct tgctctcgat gcacaccgtg gggctggagc tcgatcacca
     3001 gctgatcgct gctcccgaga cggaggggct gccggtcatg cggcgctggc acgtggtcaa
     3061 cacgctggcg aagactctct cgccggcggc cgaggcgttt cgctatttcg tgctcgagac
     3121 cggcgaagcc tttctcgcca ggcactttgg tcacggcagc gacccgaccc agttcggtga
     3181 gtggcaccat cgccggcgcc tgtgagtggg cacagctcgc gtccgagctt tagcgccgcg
     3241 ccgttctctg cccgcgcagc gagcgcgtgg cgggagtacg atgtcgtgca tgacattgat
     3301 gcttcgccgc gcggcgttgt tgatggtact cgcgagctat ccgctcgctc gcgccatggg
     3361 cgccgatgcg ccggcatcca acccgcaggt gcagttcggc ggggagtgcg c
//
LOCUS       DATDMP010000146         4455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_134429, whole genome shotgun sequence.
ACCESSION   DATDMP010000146 DATDMP010000000
VERSION     DATDMP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134429"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VI195_02415"
     CDS             <1..280
                     /locus_tag="VI195_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823268.1"
                     /translation="IADRLRQEFQMSLLASLAGLIARTVLRRAAAGLDPRRYNGACMV
                     GLSGIVVKSHGRADGVAFARAIGTAALAVRRGLISHIAQAMQAQGAVN"
     gene            277..1233
                     /locus_tag="VI195_02420"
     CDS             277..1233
                     /locus_tag="VI195_02420"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY6823269.1"
                     /translation="MIYSRVAGTGSYLPKKVITNHDLEKMVDTTDEWIRTRTGIERRH
                     IAADGETTVDLAEHAARRALETAGVAPAEVDFIAFGTTTPDLVFPNCGTLLQRRLGCR
                     GVPAFSLETACAGFMYALSIADKYVRCGEARRALVIGAETLSRITDWNDRGTAVLFAD
                     GAGAVVLEPSSAPGVLSTHLHSDGEYKDLLYCGSGVSTGFAAKLAISMAGSEVFKIAV
                     TKLGQVVDETLAANGLDRTALDWLVPHQANIRIIQATARKLEMPMERVIVTVQDHGNT
                     SAASVPLALDVAVRDGRIRRGELLLLEAFGGGFTWGSALVRF"
     gene            complement(1292..1555)
                     /locus_tag="VI195_02425"
     CDS             complement(1292..1555)
                     /locus_tag="VI195_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HEY6823270.1"
                     /translation="MIGCTDLKPLQAQVDDLKAQVAKNASDLAGAKSAADSANSAAQS
                     AGQAASGAQSTANQALAAAHASQSCCDATNEKIDRMFKRSVSK"
     gene            2084..2707
                     /gene="lexA"
                     /locus_tag="VI195_02430"
     CDS             2084..2707
                     /gene="lexA"
                     /locus_tag="VI195_02430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY6823271.1"
                     /translation="MSDLTPRQTQILRLIQRFIAETGMPPTRAEIAHELGFRSANAAE
                     EHLRALARKGVIALIPGTSRGIQLKDTIREQMGLPLIGRVAAGKPIFSEENFEARLQI
                     DPAVFQPRPHYLLKVVGMSMKDAGILDGDLVAVHRTSEVRSRQIVVARLENEVTVKRY
                     RQEGSVVWLLPENSEFEPIKVDLKEEPLLIEGIVVGVVRRGRASGLM"
     gene            2777..3397
                     /gene="imuA"
                     /locus_tag="VI195_02435"
     CDS             2777..3397
                     /gene="imuA"
                     /locus_tag="VI195_02435"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEY6823272.1"
                     /translation="MVVGDPKITRLLEHPAIWRGRSAAQRVGLSTGFAALDEHLPDNG
                     WPRTGLIEILVARFGSGELTLLMPALAALTRAAAARWCVWVAPPLLPFAPALVACGVV
                     LDRIAVVGGARSLWALEQTLGSGACDAVLGWARQPKPREIRRLQLAAERGRTLGVLFR
                     PRRAAREASAAVLRLSLEPLVAGVRLTLLKGRSAHRGVLDLPWPGS"
     gene            3578..>4455
                     /locus_tag="VI195_02440"
     CDS             3578..>4455
                     /locus_tag="VI195_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY6823273.1"
                     /translation="MHGLEELAIRAQRFTPRVSLAPPDGLLLEVAGSLHLFGGLSGLR
                     RELTGECLHLQIRPVLAFAPTPLAALTAARAGQELLIGDRSQLVGLLAPLPLGALRWP
                     EETCARLARAGVRTIGAALRLPRAGFARRFGVAQLATLDALTGRTREVRTTHRAPLRF
                     RRRHELDGEVSDHDRLRAALAPLFDALGASLTAHQCGVAELECRLVHRNVPATSCVLA
                     MAAPCANAQQLSALFAERLHSLQLPEPVRAFELRADQLLPQRSPGRGLWQPGEQGGEA
                     CAESAGLIDRLCARLGS"
BASE COUNT          703 a         1446 c         1472 g          834 t
ORIGIN      
        1 catcgcggat cggctgcgtc aggagttcca gatgtcgctg ctcgcttcgc tggccggtct
       61 catcgcgcgt acggtgctgc gccgggcggc tgccggactg gatccgcgcc ggtacaacgg
      121 ggcctgcatg gtcggactct ccgggatcgt ggtaaaaagc cacggtcgcg ccgacggtgt
      181 ggcgttcgca cgcgcgatcg gcaccgcagc gctcgccgtg cggcgcggac tgatatctca
      241 cattgcgcag gcaatgcagg cgcaaggggc ggtgaattga tctattcgcg cgtggccggt
      301 accggctcct atctgccgaa gaaagtcatc accaaccacg atctcgagaa aatggtcgat
      361 accaccgatg agtggattcg cacgcgcacc ggtatcgagc gccgccacat cgctgcagac
      421 ggcgagacca ccgtcgatct cgcggagcat gcggcgcgcc gcgcgctcga gaccgcagga
      481 gtggcgcccg ccgaggtcga cttcatcgcc ttcgggacga ccacccccga tctggtgttc
      541 cccaactgcg gcacactgct gcaaaggcgt ctcggctgcc ggggcgtccc ggccttcagc
      601 ctcgagaccg cttgcgccgg gttcatgtat gcactgtcga tcgcggacaa gtacgtgcgc
      661 tgcggagagg cccggcgtgc actggtgatc ggcgcggaga cgctctcgcg catcaccgac
      721 tggaacgacc gcggcaccgc ggtgctgttc gccgatgggg cgggtgcggt ggtgctcgag
      781 ccctcgtcgg ccccgggtgt gctctcgact catctgcact ccgacggcga atacaaggat
      841 ctgctttatt gcggcagcgg cgtctccacc ggcttcgctg ccaagctcgc catcagcatg
      901 gcgggcagcg aagtcttcaa gattgccgtc accaagctcg gacaggtggt ggacgagacg
      961 ctcgccgcca acggcctcga tcgcaccgca ctcgactggt tggtgcctca ccaggcgaac
     1021 atccgcatca tccaagcgac cgcgcgcaag ctggagatgc cgatggagcg ggtcatcgtc
     1081 accgtgcagg atcacggcaa cacctcggcc gcttcagtgc cgctcgcgct cgatgtggcg
     1141 gtgcgtgatg ggcgcatccg ccgcggagaa ctgctgctgc tggaagcctt tggaggcggc
     1201 ttcacctggg gctcggcgct cgtgcgcttc tgagcaccgc tcaaaaaaac gccgcgcaga
     1261 tgcgcggcgt ttcatctttt tgagacctgt gttacttcga taccgaccgc ttgaacatgc
     1321 ggtcgatctt ctcgttcgtc gcgtcgcagc atgactggct ggcatgcgcc gccgccaacg
     1381 cctgattggc cgtgctctgc gcaccacttg ccgcctggcc tgccgactgt gcggcactat
     1441 tggcggagtc ggctgcgctc ttggcgcccg ccagatcact cgcgttcttg gctacctggg
     1501 ccttcagatc atcgacctga gcctgcaggg gtttcagatc cgtacaacct atcattccgg
     1561 ctaccgctgc aacggcagcc gcgaccttca caacgctcgc gcacttcata aacgtcccct
     1621 tttgggttgt tgattcgaga atccttaata acggatgcga ctccggtgaa gcaagtccta
     1681 ttagccgaaa ggcaactgca ccaaatttga ctcgggaatg caactccaaa caccgatcct
     1741 gtcttttaca tgcgacagtg cagcggccgg tgcgtgtact cgtgcgaggc atgcagcgcg
     1801 cacgagttgg aagcggatct gcccgggaat tctcagcctg cagcggcagc gcgggcgata
     1861 gacacttcga tggcgaggcg catctattgg cgtgcagggc acagatgttc acttaaaaat
     1921 ttttaatggc ctcgtgggga cggcaaggat ctgtccgaac tgccccggcc acgctgccgt
     1981 gcctggcggg ggccaggcga ggcgtgtgct ttttcggcac ttgctgcaga cctcgatctg
     2041 gatataatca cagtagctgt atatttagcc aggcagctgg aaaatgtccg accttacccc
     2101 ccgacagacg cagattctgc gcctgattca gcgcttcatc gccgagactg gcatgccgcc
     2161 gacgcgggcg gagattgccc atgagcttgg gtttcgttcg gcgaacgctg ccgaggagca
     2221 tctgcgggcg ctcgcccgca agggtgtcat tgcactgatc ccgggcacct cgagagggat
     2281 acagctgaag gacacgatac gcgaacagat gggactgccg ctcatcgggc gggtggccgc
     2341 cggcaagccc attttttcag aagagaattt cgaggcgcga ctgcagatcg atccagcggt
     2401 gtttcagcca cggccgcatt acctgctgaa ggtggtcggc atgagcatga aggatgccgg
     2461 catcctcgat ggggatctgg tcgcggtgca tcgcacctcc gaggtgcgca gccggcagat
     2521 cgtcgtggcg cgactcgaga atgaggtcac cgtcaagcgc tatcgccagg agggctccgt
     2581 ggtatggctg ctgcccgaga acagcgagtt cgagccgatc aaggtggatc tgaaagagga
     2641 accgctgctc atcgaaggga tcgtcgtggg cgtggtacgg cgtggccgcg cgagcggcct
     2701 gatgtgagcg catgccgccg gctgcggcag tggctttttt tccagaggtt cgatcaaagt
     2761 tgtttcatga gctggcatgg tcgtcggcga tccgaaaatc actcgcctgc tcgagcatcc
     2821 tgccatctgg cgcggccgca gcgcggcgca gcgagtggga ttgtccaccg gatttgcggc
     2881 gctcgatgag caccttcccg acaatggctg gccacgcacc ggtctgatcg aaatcctcgt
     2941 ggctcgtttc ggcagcggcg agctgacgct gctcatgccg gcactggcgg cgctgacacg
     3001 cgcggccgcg gcacgctggt gtgtgtgggt agcaccgccg ctgctgccgt ttgcaccggc
     3061 actcgttgcc tgcggggtgg tgctcgatcg gatagccgtc gtcggaggcg cgcggtcgct
     3121 gtgggcgctc gagcagaccc tcggctcagg tgcctgcgat gcagtgctcg gatgggcgcg
     3181 gcagccgaaa ccgcgcgaga tccgtcgtct gcagctcgct gccgagcgcg gccgcacgct
     3241 cggggtgttg tttcgtccac ggcgcgcggc gcgcgaggcc tccgccgcgg tgctgcgctt
     3301 gagcctcgag ccgttggttg ccggggtgcg gctcactctc ctcaaggggc gcagcgctca
     3361 tcgcggtgtg ctcgatcttc cctggcctgg cagttgagtc atgcgcccgc acctcatcgg
     3421 ctcagccgcc aaaacggcag acctgccgtc cgccatagcc cctgcccgga cggacccggc
     3481 accgtcgcct gcgccactgc gcagcgtgcg gcgcgcgctg catggctcat ccaggagcga
     3541 gctttgggcc ggactgcacc tgagcggact ggagccggtg cacggactcg aggagctggc
     3601 catccgggcg caacgcttca ctccacgcgt gagtctggca ccgcccgatg gcctgctgct
     3661 cgaggtcgcc ggaagcctgc acctgttcgg cggactgtcg ggtctgcggc gtgagctcac
     3721 cggggaatgc ctgcatcttc agatccggcc cgtgctcgct ttcgcaccca caccgcttgc
     3781 cgcgctcact gcggcgcgcg ccggccagga gctgctcatc ggcgatcggt cacagctcgt
     3841 cggtttactc gcgcctctgc cgctcggcgc tctgcgctgg cccgaggaaa catgtgcacg
     3901 tctcgcccgc gccggtgtgc gcactattgg cgccgcactg cggctacccc gtgccggttt
     3961 cgcgcgccgc ttcggcgtgg cgcagctcgc cactctcgat gcgctcacgg gacgcacccg
     4021 ggaggtgcgc acgacgcatc gagcaccgct gcgctttcgc cgccgccacg agctcgacgg
     4081 cgaagtgagc gatcatgacc ggttgcgcgc ggcactcgcg ccgctgttcg atgcactcgg
     4141 cgcttctctc accgcgcatc agtgtggcgt ggcggagctc gaatgccggc tcgtgcaccg
     4201 caatgttccg gcgacgagtt gcgtgctcgc gatggcggca ccctgtgcca acgcgcagca
     4261 gctcagtgcg ctttttgccg agcgcctgca ttcgctgcag ctgcccgagc cggtacgcgc
     4321 gttcgagctg cgagcggatc agctgctacc gcaacggtcg cccggtcgcg ggctgtggca
     4381 acctggcgag cagggcggtg aggcgtgtgc ggaaagtgca ggtctcatcg atcgcctgtg
     4441 cgctcgttta ggctc
//
LOCUS       DATDMP010000147         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_134447, whole genome shotgun sequence.
ACCESSION   DATDMP010000147 DATDMP010000000
VERSION     DATDMP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134447"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VI195_02445"
     CDS             complement(<1..1060)
                     /locus_tag="VI195_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6823274.1"
                     /translation="MALRVQSADWGARAISAQRPGHALSREFYCNEELFEREVRQLLL
                     RHWHCVGHQSQLREPGDFFTTSFCGEPLLIVRGQDHELRALLNVCRHRGSRVCTQESG
                     HVRGGVFVCPYHAWSYGLDGRLRAARHMPDDFDRAAHGLKSLHTRVIEGLIFVSFAES
                     PLGLEHVEAALAGSARRYGWAQAQVAHRESYPVRANWKLAVENYMECYHCGPAHEEYS
                     RFHLYARPAPLNRSADERVRERARALGVTLTEMDYWGLNARAGQEAADSVRSALGEGA
                     VSGSEDGRPLAPLMGRFSDYDGGVTFFDIGLTSSFLAYPDHGLIYRFVPNAVQDTDME
                     VIWLVREDARQGVDYDLER"
     gene            complement(1060..>2672)
                     /locus_tag="VI195_02450"
     CDS             complement(1060..>2672)
                     /locus_tag="VI195_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6823275.1"
                     /translation="VSYYNRKQLTAYQGTVYDLSYWNTIPSGMGGPNDSFPLPPKCTA
                     PDDATSCSWFPLIDANGIHLPAALRDTQTPNTMTNTQKSYTAELRLQSNNPSSRWTWT
                     AGVFWQQAKEGSIEELKSTNVDQVFNYLFGLTPAVYYSPDGVTPANFYSCPTNAAYPI
                     IPACDIYYNNNNTIDRQIAGFGEVSFAFTDWMRLTVGERIAHTTFSLEHYADGYENYG
                     PGPASASQSETPNTPKAVLSFQVDPRNLYYLSYAKGFRVGGGNAPLPPYCDADLAAAG
                     YPNGAPLTYQSDSTQNYEVGSKNGIGDWLRVATSVYYIEWHNIQQNLYVAGYCGLQFT
                     DNLGTAVAKGFDLQAQMLFGPISIDLATGYTDARYSKNSPVDCTPGSSDPSTGIPCKA
                     VTGDAISGQEGINYAPGTMPPWTVSLGLQYNFRLADRDTFVRADWEYQSRSPWLANVQ
                     DPKAGAVYFYGYSYTYPSTKYTSLRAGMNFGDVQLTAFCENLFDAHPVTNYILGQTDG
                     TYSPQQNTYTFRPRTFGLNLIWHVGAEH"
BASE COUNT          452 a          839 c          894 g          487 t
ORIGIN      
        1 gccgttccag atcgtagtcg acgccctgcc gcgcgtcctc gcgcaccagc cagatgactt
       61 ccatgtcagt gtcctgcacc gcgttgggca cgaagcggta gatgagtccg tgatcggggt
      121 aggcgaggaa gctgctggtc agtccgatgt cgaagaaggt gacgccgccg tcgtaatcgg
      181 agaatcgccc catcagtggc gccagcggcc gaccgtcctc actgccgctc accgcaccct
      241 cgccgagcgc ggaacgtaca ctatcggctg cttcctgccc ggcccgggcg tttaaccccc
      301 agtagtccat ctccgtgagc gtcacaccga gcgcccgcgc ccgctcgcgc acccgctcgt
      361 ccgcgctgcg gttcagcggc gcgggacgcg catacaggtg aaagcgcgag tactcctcat
      421 gcgccggccc gcagtggtag cactccatgt aattctctac cgcgagcttc cagttggcgc
      481 gcaccggata gctctcgcgg tgcgccacct gcgcctgcgc ccaaccgtag cggcgcgccg
      541 atcctgcgag ggccgcctcg acgtgctcga gcccgagcgg ggattcggca aaagatacga
      601 aaatcagccc ctcgatgacg cgggtatgca ggctcttgag gccgtgggcc gcgcgatcga
      661 agtcatccgg catatgccga gccgcgcgca gccgcccgtc gagcccgtag ctccaggcgt
      721 ggtagggaca cacgaacacc ccaccacgga catggccgga ctcctgcgtg cagacgcgcg
      781 agccgcggtg ccggcagaca ttcagcagcg cgcgcagctc gtgatcctgc ccacgcacga
      841 tgagcagcgg ctcgccgcag aagctcgtgg tgaagaaatc gcccggctcc cgcagctggc
      901 tctggtgccc gacgcaatgc cagtggcgca gcaggagttg ccgaacctca cgctcgaaga
      961 gctcctcgtt gcaatagaat tcgcggctga gcgcgtgccc agggcgctgg gcacttatcg
     1021 cccgcgcgcc ccaatccgcg ctctgcacgc gcagcgccat cagtgctccg ctcccacgtg
     1081 ccagatcaga ttgagcccga aggtgcgcgg ccggaacgtg taggtgttct gctgcggcga
     1141 gtacgtgcca tcagtctggc cgaggatata gttggtcacg gggtgcgcat cgaacaggtt
     1201 ttcgcagaac gccgtgagct gcacgtcgcc gaagttcatg cctgcgcgca gcgaggtgta
     1261 tttggtcgac gggtaggtgt aggaatagcc gtaaaagtac accgcgccgg ccttcgggtc
     1321 ctgcacgttc gcgagccacg gactgcgact ctggtactcc cagtccgcgc gcacgaacgt
     1381 gtcgcggtcg gccagccgga agttgtattg caggcccaac gacaccgtcc acggcggcat
     1441 cgtccctggg gcgtagttga tgccctcctg accgctgatg gcatcgccag taacagcctt
     1501 gcacggtatg ccggtgctcg ggtccgagct ccccggagtg cagtcgaccg gggagttctt
     1561 gctgtagcgc gcatcggtgt agccggtggc cagatcgatg ctgatggggc cgaagagcat
     1621 ctgcgcctgc aggtcgaacc ccttggcgac cgcggtgcct aggttgtcgg tgaactgcag
     1681 gccacagtac ccggcgacgt agagattctg ctggatgttg tgccactcga tgtagtagac
     1741 gctggtggcc acccgcagcc aatcgccgat gccgttcttg gagccgactt cgtagttctg
     1801 cgtagagtcc gactggtaag tgagcggcgc cccgttcggg tatccggcgg cggccaggtc
     1861 cgcatcgcag tagggcggca gcggcgcgtt gccgccaccg acccggaaac ccttggcgta
     1921 gctcagatag tagaggttgc gggggtcgac ctggaaggag agcaccgcct tgggtgtgtt
     1981 gggcgtctcg ctctggctcg cagaggccgg ccccggtcca taattctcat agccgtccgc
     2041 gtagtgctcg agcgagaacg tggtgtgcgc gatgcgctcc ccgacggtga ggcgcatcca
     2101 gtcggtgaag gcgaagctca cctcgccgaa gccggcgatc tggcggtcga tagtgttgtt
     2161 gttgttgtag tagatgtcgc aggccgggat gatcgggtag gcggcattgg tcgggcagga
     2221 atagaaattg gcgggggtaa ctccgtcggg agaatagtag accgccggag tcaagccaaa
     2281 gaggtagttg aacacctggt cgacgttggt cgacttcagc tcctcgatgc tgccctcctt
     2341 cgcctgctgc cagaagacac ccgcggtcca ggtccagcgc gagctggggt tgtttgactg
     2401 caagcgcagc tccgcggtgt agctcttctg ggtgttcgtc atggtgttgg gcgtctgcgt
     2461 gtcgcgcagc gccgccggca gatggatccc attggcatcg atcaacggga accaggagca
     2521 gctggtcgca tcgtccggcg ccgtgcattt gggcggcaac gggaaggaat cgttcggtcc
     2581 tcccatccca ctcgggatgg tgttccagta gctcaggtcg tacaccgtgc cctggtaagc
     2641 ggtgagctgc ttgcggttgt agtaggaaac gt
//
LOCUS       DATDMP010000148         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_467728, whole genome shotgun sequence.
ACCESSION   DATDMP010000148 DATDMP010000000
VERSION     DATDMP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467728"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VI195_02455"
     CDS             <1..775
                     /locus_tag="VI195_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY6823276.1"
                     /translation="AFTGADAQRRGRFEQADGGTLFLDEIGDMSLPLQTRLLRVLAEG
                     EFYRVGGQTPIHVDVRVIAASNQNLQERVARGLFREDLYHRLNVIRIELPPLSARAED
                     IPALLTHYMLIAAHELGVEPKVLAPDAQARLAAYGWPGNVRELVNLCRRLSVLAPGSE
                     VHLADLPPEITGSAAAAPKEADWAAALADWAERQVRNGGAPLLDAAQPQFERVLIRAA
                     LRRTQGHRQDAAKLLGWGRNTLTRKLRELGMNGAADADE"
     gene            823..1941
                     /locus_tag="VI195_02460"
     CDS             823..1941
                     /locus_tag="VI195_02460"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY6823277.1"
                     /translation="MSGWSELWHSEFLGNQLGEWAVSLAIFLITFTALPLVRGFISAQ
                     RRRLHLKDRQGGYLALDLAAALVEHTNQLFLWGLAVWLGSRDLTFPPRLERWLTSVLV
                     LLFWMQMAIWAMTAVRHAIDLRRQRSMSPDALLKSSMEVIVFAVGLLIWGFAALLALD
                     SLGIQVKPLLAGLGITGIALALAVQTVLGDLLASMSIAFDKPFMPGDFLAIDDCQGTV
                     EHIGVKSTRLRSVNGEQIVLGNADILKSRMRNFGRMAERRALFVLDVHYETPVAALAA
                     VPGAVREIVEATPQVRFDRCHLLRCTATALQFEVVFFVTTPDYQVYASAQQSINLRIL
                     ERFRTLGVTFLAAAPTPVRLHRQPNAAAADDGGQRRLL"
     gene            complement(2009..>2835)
                     /locus_tag="VI195_02465"
     CDS             complement(2009..>2835)
                     /locus_tag="VI195_02465"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY6823278.1"
                     /translation="VLKGTTGAARLGNPPHRVYETPAGMLNAIGLQNPGVDHVVRVIL
                     PQLDFSETRFIANACGSTLEEYVEVTRRFDASPIDAIEINISCPNVKEGGVAFGNYPD
                     MSAQVVAACRRVTKKPLITKLSPNQTDIRENARRCIEAGSDALAVVNTVMGMAIDVET
                     RRPVIGNVQGGLSGPAIKPIALLKVHQVYEVAGPHRIPIIGQGGITTAQDALEFIIAG
                     ATAVGVGTALFYDPLVCRRINAGIAAYLTAHGLKSVTELVGTMRTARDIADCALSG"
BASE COUNT          425 a          948 c          978 g          484 t
ORIGIN      
        1 ggctttcacc ggggcggatg cccagcggcg cggccgcttc gagcaggcgg atggcggcac
       61 gctctttctc gatgagatcg gcgacatgtc gctgccgctg cagacgcggc tgctgcgcgt
      121 gctggcggaa ggggagttct atcgtgtcgg cggtcagacc ccgatccacg tcgatgtgcg
      181 cgtcatcgcc gcgagcaacc agaacctgca ggagcgggtc gcccgcgggc tgttccgcga
      241 ggatctctac catcgcctca acgtgatccg catcgagctg ccgccactat ccgcgcgcgc
      301 cgaggacatt ccggcgctgc tcacgcacta catgctgatc gcggcgcacg agctcggcgt
      361 cgagccgaaa gtgctcgccc cggacgcgca ggccagactc gccgcctacg gctggccggg
      421 caacgtgcgc gagctcgtca acctgtgccg gcggctctcg gtgctggcgc ccgggagcga
      481 ggtgcacctc gctgatcttc cgcccgagat caccggcagc gctgcggccg caccgaagga
      541 ggctgactgg gcggccgcgc tcgccgactg ggccgagcgg caggtgcgca atggcggcgc
      601 gccgcttctc gatgcggcgc agccgcaatt cgagcgggtg ctgatccgcg ccgcgctgcg
      661 tcgcacccag gggcatcgcc aggacgcggc gaagctgctc ggctggggac gcaacaccct
      721 cacccgcaag ctgcgcgagc tcggcatgaa cggcgccgcg gacgccgacg aatagtcgcg
      781 acgctgctaa cccggtaagg tttcgccccg aagggagggc cgatgtcggg ctggagcgag
      841 ttgtggcatt ccgagttcct gggtaaccag ctcggtgagt gggcggtctc gctcgcgatc
      901 ttcctcatca ccttcaccgc actgccgctc gtccgcggct tcatcagcgc gcagcgccga
      961 cgtctgcacc tgaaggaccg acagggtggt tacctggcgc tcgacctggc ggccgcgctg
     1021 gtcgagcaca ccaaccagct gttcctgtgg ggcctcgccg tctggctggg ctcccgcgat
     1081 ctgaccttcc cgccgcggct cgagcgttgg ctgacgagcg tactcgtgct gctcttctgg
     1141 atgcagatgg cgatctgggc catgaccgct gtgcggcacg ccatcgacct gcgccggcaa
     1201 cgctcgatgt cgcccgatgc actcctcaag agctcgatgg aagtcatcgt gttcgcggtt
     1261 ggactcctga tctggggctt cgctgcgctg ctggcgctcg actcgctcgg catccaggtg
     1321 aaaccgctgc tcgcaggctt ggggatcacc ggcatcgcgc tcgcgctggc cgtgcagacg
     1381 gtgctcgggg acttgctggc gtcgatgtcg atcgccttcg acaagccgtt catgccgggt
     1441 gatttcctgg cgatcgatga ctgtcagggc acggtcgagc acatcggcgt gaagagcacg
     1501 cggctgcgca gtgtcaacgg cgagcagatc gttctcggca acgccgacat cctgaagtca
     1561 cggatgcgca acttcggccg catggccgaa cggcgcgcgc tgttcgtgct cgacgtgcac
     1621 tacgagaccc cggtggcggc gcttgcggca gtccccgggg cagtgcgcga gatcgtcgag
     1681 gcaaccccgc aggtgcgctt cgaccgctgc cacctcctgc gctgcaccgc gaccgcgctg
     1741 cagttcgagg tggtgttttt cgtcacgaca cccgattacc aggtgtacgc cagcgcgcag
     1801 cagagcatca acctgcgcat cctcgagcgc ttccgcacac ttggagtcac gtttctggcc
     1861 gccgcgccca ctccggtgcg gctgcaccgg cagccgaacg ccgcggctgc ggacgatggc
     1921 ggccagcggc ggctgctcta agcagtgcga gagatcatgc tcaggacagg cagcagcggg
     1981 ccctgcgccg ccgggctaga tgcatgatct agcccgagag tgcgcagtcc gcgatgtccc
     2041 tggcggtgcg catcgtgccg acgagctcgg tgacgctctt caggccgtgc gcggtgagat
     2101 aggcggcgat gcctgcgttg atgcgccggc acacgagcgg atcatagaag agtgcggtgc
     2161 ccaccccgac cgcggtcgcg ccggcgatga tgaactcgag cgcatcctgc gccgtggtga
     2221 tgccgccctg gccgatgatc gggatgcggt gcgggccggc gacctcgtag acctggtgca
     2281 ccttcagcag cgcgatcggc ttgatggcgg gcccggaaag tcccccctgc acgttgccga
     2341 tcaccggacg gcgcgtctcg acatcgatcg ccatccccat cacggtgttg accaccgcga
     2401 gcgcgtcgct ccccgcctcg atgcaacggc gcgcgttctc gcggatgtcg gtctggttcg
     2461 gcgagagctt ggtgatgagc ggtttcttgg tcacgcggcg gcaggcggcg accacctgcg
     2521 ccgacatgtc cgggtagttg ccgaaggcca ccccgccttc cttcacgttg gggcaggaga
     2581 tgttgatctc gatggcatcg atgggcgagg catcgaagcg ccgcgtgacc tcgacgtact
     2641 cctcgagggt agagccgcag gcgttggcga tgaagcgtgt ctcgctgaaa tcgagctgtg
     2701 gaaggatgac gcgcacgacg tgatcgacgc ctggattctg gagcccgata gcattcagca
     2761 tgccggccgg cgtctcgtac acgcggtgcg gcggattgcc gaggcgtgca gctccggtcg
     2821 tacccttgag cacga
//
LOCUS       DATDMP010000149         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_134893, whole genome shotgun sequence.
ACCESSION   DATDMP010000149 DATDMP010000000
VERSION     DATDMP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134893"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /gene="glmM"
                     /locus_tag="VI195_02470"
     CDS             complement(<1..1092)
                     /gene="glmM"
                     /locus_tag="VI195_02470"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY6823279.1"
                     /translation="MERRYFGTDGVRGRVGEHPMTVDFALKLASAAARVLAPEGGTVL
                     IGKDTRLSGYMFESALEAGFVAAGVNAMLIGPLPTPGIAYMTRRFNCDFGVVISASHN
                     LYDDNGIKFFDASGAKLSDALEERIEAELEQPPLTCASSDLGRATRVDRSRTLYQEFC
                     TSTLPAGVDLSGLKIVVDCANGAAYKVGPRILADLGAEIVPIGCSPNGRNINDGCGST
                     APDLLQLTVPGVRANVGLALDGDGDRLVMVDHLGRIVDGDQVLYVIARSLQRSGTLRG
                     PVVGTVMSNLGLELALRSAGIEFRRAAVGDRYVLSMLREAQGVLGGETSGHILCLDKT
                     TTGDGLVVALQVLAVMKQTGRTLAELTSAM"
     gene            complement(1092..1943)
                     /gene="folP"
                     /locus_tag="VI195_02475"
     CDS             complement(1092..1943)
                     /gene="folP"
                     /locus_tag="VI195_02475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY6823280.1"
                     /translation="MSSAPVLLRCADKTLDLSRAVVMGVLNVTPDSFSDGGRFAAHDA
                     AIEHGLRMAAEGAALIDVGGESTRPGAQAVTLQEELRRIVPVIEQLRRATTAIISVDT
                     SKPEVMRAAAAAGAGFINDVRALREPGALEAAAASRCGLCLMHMQGEPRTMQAAPHYQ
                     DVLAEVRAFLGGRVAACRAAGIDPLRLSVDPGFGFGKNLEHNLTLLRHLPELVADGPP
                     VLVGLSRKSTVGKLTGREPGERLYGSVALAVIAALKGARIIRAHDVAATVDALQVTAA
                     VAAEGGD"
     gene            complement(2179..>3546)
                     /gene="ftsH"
                     /locus_tag="VI195_02480"
     CDS             complement(2179..>3546)
                     /gene="ftsH"
                     /locus_tag="VI195_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY6823281.1"
                     /translation="QKFQKLGGRIPKGVLLVGPPGTGKTLLARAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLN
                     QLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQILRVH
                     MRRVPLADDVKPALIARGTPGFSGADLANLVNEAALFAARANKRIVGMEEFERAKDKI
                     MMGTERRSMVMSEAEKRMTAYHEAGHAIVGMTVPEHDPVYKVTIIPRGRALGVTQFLP
                     EQDRYSFSKRRLESAIATLFGGRIAEELIFGPESVTTGASNDIERATELARNMVTKWG
                     LSDKLGPLTYSEETGEVFLGRSVTQHKQVSDVTAHAIDEEVRRVIEGSYQRARQILTT
                     ALEKLHLMADALMKYETIDEEQLKDIMAGREPKPPAGWDDSLSNRPPTAPAPAGTPVI
                     PAGQH"
BASE COUNT          585 a         1209 c         1176 g          576 t
ORIGIN      
        1 catcgcggag gtgagctccg cgagtgtgcg gccggtctgt ttcatcaccg cgagcacctg
       61 cagtgccacg acgagcccat cgccggtcgt cgtcttgtcg aggcagagaa tgtggcccga
      121 ggtctcaccg ccgagaaccc cctgggcctc gcgcagcatc gacagcacgt agcgatcgcc
      181 gaccgcggcg cggcggaatt cgatgccggc tgagcgcagc gccagttcca gaccgagatt
      241 gctcatcacc gtgccgacca ccgggccgcg cagcgtgccc gagcgctgca gcgaacgcgc
      301 gatgacatag agaacctggt cgccatccac gatcctgccc aggtgatcca ccatgaccag
      361 ccggtcgcca tcgccatcga gggcgagccc cacgttggcg cgtaccccgg gcacggtgag
      421 ctgcagcaga tccggcgccg tcgagccgca gccgtcgttg atgttacggc cgttggggga
      481 gcaaccgatc ggcacgatct cggcgcccag gtcggcgagg atgcgcggcc cgaccttgta
      541 ggcggcgccg ttcgcgcagt cgacgacgat cttcaatccc gacaggtcga ctccggcggg
      601 cagggtcgag gtacagaatt cctgatacag ggtacgcgag cggtcgacgc gcgtcgcacg
      661 gccgaggtca ctcgaggcgc aggtgagtgg gggctgctcg agctccgcct cgatgcgctc
      721 ctcgagcgca tcggagagct tggcacccga agcatcgaag aacttgatac cgttgtcgtc
      781 gtagagattg tgcgaggcgc tgatgacgac gccgaagtcg cagttgaagc gccgcgtcat
      841 gtaggcgatg cccggggtcg gcagcggtcc gatcagcatg gcgttcaccc cggccgccac
      901 gaatccggcc tcgagcgccg attcgaacat gtaacccgag agccgggtgt ccttgccgat
      961 cagcaccgtg ccgccctcgg gcgcgagcac gcgcgccgcg gcgctcgcga gcttgagcgc
     1021 aaaatcgacc gtcatcgggt gctcgccaac gcgccccctg actccatcgg taccgaaata
     1081 gcgacgctcc atcagtctcc accttctgcg gccaccgccg cggtcacctg cagggcatcg
     1141 accgtcgcgg ccacatcatg ggcacggatg atgcgcgcgc ccttgagcgc ggcgatcacc
     1201 gcgagcgcca cgctgccata gaggcgctcg cccggctccc gcccggtgag tttgccgacg
     1261 gtcgacttgc gcgacaaccc gaccaacacc ggcggcccgt cggccaccag ctcgggcagg
     1321 tgccgcaaca gcgtcagatt gtgctccagg ttcttgccga aaccgaatcc gggatcgacg
     1381 ctgagccgca gcggatcgat tccggccgcg cggcaagccg cgacgcggcc gccgagaaag
     1441 gcacgcacct ccgccaacac atcctggtaa tggggcgcag cctgcatggt gcggggctcc
     1501 ccctgcatgt gcatgagaca taagccgcag cggctggcgg cggcggcctc gagcgctccg
     1561 ggctcacgca gcgcgcgcac gtcattgatg aagccggcgc cggccgcggc ggcggcgcgc
     1621 atgacctcgg gcttactggt gtcgacggaa atgatcgccg tggtggcgcg gcgcagctgc
     1681 tcgatgaccg ggacgatacg gcgcaactcc tcctgcagcg tgactgcctg ggcaccgggg
     1741 cgggtggatt ccccgccgac atcgatgagt gcggcaccct ccgctgccat gcgcaacccg
     1801 tgctcgatcg ccgcatcgtg tgcggcaaag cgcccgccgt cggagaagga gtcgggcgtg
     1861 acgttgagca cacccatgac gaccgcgcgg ctcagatcga gcgtcttatc ggcgcagcgc
     1921 agcaggaccg gcgcagagga catcgcagag gacatcgagg acgtgacgtt acccccgccc
     1981 cgaggccatc gtggccatgg tggccgtcgt ggccatggtg gccgtcgtgg ccatggtggc
     2041 catcgtggcc atggtggcca tcgttaagac acgtttcccg aatagttaac caaatggtga
     2101 actatccgca aagataccct cggcggtgcc ggcccggtgc cggtccggtg ccggcgggca
     2161 gcgaggacga tgctgctgtt agtgttggcc ggccggtatg accggcgtgc ccgccggagc
     2221 aggcgcagtc ggcggccggt tggacaacga atcgtcccag cccgcaggcg gcttcggctc
     2281 ccggcccgcc atgatgtcct tcagctgttc ctcatcgatg gtctcgtact tcatgagcgc
     2341 atcggccatg agatgcagct tctcgagcgc ggtcgtgagt atctgccgcg cacgctggta
     2401 gctaccctcg atgacgcgac gcacctcctc gtcgatggcg tgcgcggtga catcggaaac
     2461 ctgcttgtgc tgagtcacgc tgcgcccgag gaacacttcc ccggtttcct ctgagtaggt
     2521 gagcggcccg agcttgtccg acaggcccca cttggtgacc atgttgcgcg cgagctcggt
     2581 ggcccgctcg atgtcattcg atgcaccggt ggtcaccgat tccggcccga agatcagctc
     2641 ctcggcgatg cgtccgccga agagcgtggc gatagcgctc tctaggcggc gcttggagaa
     2701 gctgtagcgg tcctgctcgg gcaggaactg cgtcacccca agcgcgcgac cgcgcggaat
     2761 gatggtcacc ttgtagaccg ggtcgtgctc ggggaccgtc atgccgacga tcgcatgtcc
     2821 tgcctcgtga taggcggtca tgcgcttttc cgcctcgctc atgaccatgg agcgccgctc
     2881 ggtgcccatc atgatcttgt ccttggcgcg ctcgaattcc tccatgccga cgatgcgctt
     2941 gttggcgcgc gcggcaaaca gtgccgcctc gttcaccagg ttggcgagat cggcgccgga
     3001 gaagcccggc gtgccgcgcg cgatcaacgc cggcttgaca tcgtccgcca gcggaacgcg
     3061 ccgcatgtgc acgcgcagga tctgctcgcg gccgcgcaca tcgggcagcg gcacgaccac
     3121 ctgtcggtcg aaacgacccg gccgcagcag cgccgggtcg agcacatccg gccggttggt
     3181 tgccgcgatg acgatgatgc cttcgttccc ctcgaagcca tccatctcca ccagcagctg
     3241 attcagtgtc tgctcacgct catcgtggcc gccgcccaga cccgcgccgc gatgccgccc
     3301 gacggcgtcg atctcgtcga tgaagatgat gcacggggcg tgcttcttcg cctgctcgaa
     3361 catgtcgcgc acgcgtgagg caccgacgcc gacgaacatc tcgacgaagt ccgagcccga
     3421 aatggtgaag aagggcacct tggcctcacc ggcgatggcg cgcgccagca gcgtcttgcc
     3481 ggttccggga ggtccaacca gcaggacgcc cttggggatg cgcccaccca gtttctggaa
     3541 cttttg
//
LOCUS       DATDMP010000150         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_912749, whole genome shotgun sequence.
ACCESSION   DATDMP010000150 DATDMP010000000
VERSION     DATDMP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912749"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VI195_02485"
     CDS             <1..321
                     /locus_tag="VI195_02485"
                     /inference="COORDINATES: protein motif:HMM:NF022789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEY6823282.1"
                     /translation="FLIGGVVVSLFSVLGDLFRPKSFAGLFDAAPSVALATLALAVIK
                     EDAIYAGIESRSMIAGAAALLVYSQLTSWLLMRGRLSSLAASLIALPLWFAAAFLLWA
                     MFLR"
     gene            324..749
                     /locus_tag="VI195_02490"
     CDS             324..749
                     /locus_tag="VI195_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEY6823283.1"
                     /translation="MVIKAQFSRVRQTKWWEYLIRFFFGGLVTVAAGLVAKEFGAVVG
                     GLFLAFPGIFPSGVTLVERHEREKKRKKGLRGERRARLVSGVVSAGAVLGSIGLLAFA
                     ALGAWLFGLLASWEVIALATAAWLAVSVLAWWLRLRALS"
     gene            complement(742..1938)
                     /locus_tag="VI195_02495"
     CDS             complement(742..1938)
                     /locus_tag="VI195_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY6823284.1"
                     /translation="MNAPEKRSKAQFNWQDPLLLDEQLTQDERMVREAARAYCQGKLA
                     PRVLEAFRHEKTDPAIFREMGELGLLGPTIPSEYGGPGLNYVSYGVIAREVERVDSGY
                     RSMMSVQSSLVMLPIFEFGNAQTKKKYLPKLASGEWIGCFGLTEPNHGSDPQSMQTRA
                     KKTPGGYSLTGTKMWISNSPIADVFVVWAKDDEGAIRGFVLEKGMKGLSAPAIHGKVG
                     LRTSITGEVVMDGVFCPEENAFPEVRGLKGPFTCLNSARYGIAWGALGAAEDCWHRAR
                     QYVLDRKQFGRPLAANQLVQKKLADMQTEITLGLQGVLRLGRMKDEGTASPEITSIMK
                     RNSCGKALEIARVARDMLGGNGISDEFGIIRHVVNLEVVNTYEGTHDIHALILGRAQT
                     GIQAFN"
     gene            complement(1964..>2794)
                     /locus_tag="VI195_02500"
     CDS             complement(1964..>2794)
                     /locus_tag="VI195_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY6823285.1"
                     /translation="VARLLSAKLSEGLKQQVVVDNRPGAGATLGTEQAARAPADGYTL
                     LITSFPSITTGPLTNPNVRYDPIEDFTHLALLGTFPNALVVRADSPLHSVAGLVAYAK
                     ANPGKVTYGSAGPASSGHMTGQLLAREAGIDIVHVPYKGAGPAFLDLLGGQIAADFDG
                     MINAAKQAEAGKVRMLAVSSAARLASHPQLPTIAETVPGVSGESWFGVAAPAGLAQPL
                     AARLESEITRAMAQPDVRARLGETGMTLSELRGAAVPEFIRADIRKWSPIASALRGSK
                     "
BASE COUNT          380 a          918 c          961 g          535 t
ORIGIN      
        1 ttcctcatcg gcggcgtcgt ggtttcgctg ttctcggtgc tcggggatct ctttcgaccc
       61 aagagcttcg cggggctctt cgatgccgcg ccgtcggtgg cgctcgcaac gcttgcgctg
      121 gcggtgatca aggaagacgc catatacgcc ggcatcgagt cccgctcgat gattgcgggc
      181 gccgcggcgc ttctcgtcta ttcgcagctc acgagctggc tgctgatgcg cggcaggctg
      241 tccagcctgg cggcctcgct catcgccttg ccgctctggt tcgccgccgc gttcctgctg
      301 tgggcgatgt tcctgagata agcatggtga tcaaggccca gttctcgcgc gtgcgacaga
      361 cgaaatggtg ggagtacctc atccgctttt tcttcggcgg ccttgtcacc gtcgcggccg
      421 ggctggtcgc aaaggaattc ggcgcggtag taggcgggct gtttctcgcg ttccccggca
      481 ttttcccctc gggggtgacc ctggtggagc ggcacgagcg ggaaaaaaag cggaagaagg
      541 gcctgcgggg cgagcgccgc gcgcgcctcg tgtcgggcgt cgtttcggcg ggcgcggtgc
      601 tgggcagcat cgggctcctc gcctttgccg ccctcggcgc gtggctcttc gggctgctcg
      661 cttcctggga ggtgatcgcg ctcgccaccg cggcgtggct ggcggtctcg gtgcttgcct
      721 ggtggctgcg cctgcgcgcg cttagttgaa ggcctgaatt cccgtctgcg cgcgcccgag
      781 gataagggca tggatgtcgt gggtgccttc gtaggtgttg accacctcga ggttcacgac
      841 gtggcggatg atgccgaact cgtcggagat tccgttgccg ccgagcatgt cgcgcgccac
      901 gcgcgcgatc tcgagcgcct tgccgcagga attcctcttc atgatggagg tgatctcggg
      961 cgaggcggtg ccttcgtcct tcatcctgcc caggcgcagc acgccctgca ggccgagcgt
     1021 gatttcggtc tgcatgtcgg cgagcttctt ctgcaccagc tggttcgccg ccagcggccg
     1081 cccgaactgc ttgcgatcga gcacgtattg ccgtgcgcgg tgccagcagt cttccgcggc
     1141 gccgagcgcg ccccaggcga tgccatagcg cgcggaattc aggcaggtga acgggccctt
     1201 gagcccgcgc acttccggga acgcgttctc ctcggggcag aacacgccgt ccatcaccac
     1261 ttcgccggta atggaggtgc gcagtcccac cttgccgtgg atcgccggag cgctcaggcc
     1321 cttcatcccc ttttcgagca cgaagccccg gatcgcaccc tcgtcgtcct tcgcccagac
     1381 cacgaacaca tcggcgatgg gggagttcga gatccacatc ttggtgccgg tgaggctgta
     1441 gccgccgggc gttttcttcg cgcgcgtctg catcgactgg ggatccgagc cgtggttggg
     1501 ctcggtgagg ccgaagcatc cgatccattc gccgctcgcg agcttcggca gatatttctt
     1561 cttggtttgc gcattcccga actcgaagat ggggagcatg accagggacg actgcacgct
     1621 catcatggag cgatagcccg agtccacccg ctcgacttcg cgcgcgatga cgccgtagct
     1681 cacgtagttg agtcctggac cgccgtattc gctcgggatg gtgggcccga gcaggccgag
     1741 ctcgcccatc tcccggaaaa tcgccggatc ggttttctcg tgcctgaacg cctccagcac
     1801 ccgcggcgcg agcttcccct ggcaataagc ccgcgccgcc tcgcggacca tccgctcgtc
     1861 ctgggtgagc tgctcgtcga ggagcagcgg gtcctgccag ttgaattggg ccttgcttct
     1921 cttctcgggc gcgttcatgg cgttcctttg gatagcagcg attctacttt gagccgcgca
     1981 gcgcgctcgc gatgggggac catttgcgga tgtcggcgcg gatgaactcc ggcacggcgg
     2041 cgccgcgcag ctcgctcagc gtcatgccgg tctcaccgag ccgcgcgcgc acgtcgggct
     2101 gcgccatcgc gcgggtgatt tcggactcga gccgcgccgc gagcggctga gcgaggcccg
     2161 cgggcgccgc cacgccgaac catgactcgc ccgagacgcc cggcacggtc tcggcgatcg
     2221 tcgggagctg cgggtgcgaa gcgaggcgag ccgccgagct caccgcgagc atgcgcacct
     2281 tgccggcttc ggcctgcttc gcggcgttga tcatcccgtc gaagtcggcg gctatctgcc
     2341 cgcccagcag atcgaggaat gccggccccg cgcccttgta tggcacgtgg acgatgtcga
     2401 tgccggcctc gcgcgccagc agctggccgg tcatgtggcc cgaggacgca gggccggcgg
     2461 agccgtacgt gacttttccg ggatttgcct tcgcgtaggc cacgagccct gccacggaat
     2521 gaagcggcga gtccgcccgg acgacgagcg cgttcgggaa ggtgcccagc agcgcgaggt
     2581 gggtgaagtc ctcgatggga tcgtagcgga cgttcggatt ggtgagcggc ccggtcgtga
     2641 tggagggaaa ggacgtgatg agcagcgtgt aaccgtctgc cggcgcgcgt gccgcctgct
     2701 cggtgccgag cgttgccccc gcgcccggac ggttgtccac caccacctgc tgcttcaggc
     2761 cttcggagag cttggccgag agcaggcgcg cgac
//
LOCUS       DATDMP010000151         3699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_690621, whole genome shotgun sequence.
ACCESSION   DATDMP010000151 DATDMP010000000
VERSION     DATDMP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690621"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1957)
                     /locus_tag="VI195_02505"
     CDS             complement(<1..1957)
                     /locus_tag="VI195_02505"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823286.1"
                     /translation="MSSPSWRELKRRGRSARTLAMRATAFPAVLAALAACSGGWHIGS
                     NSQVTDPATVDYPVFYVKRQVPVDQAGLLVQDDLRIMRTLTPSTPSADLYMRKSASPD
                     AAEINISARITAGQMWDVKDVDTSADGSRVIFAMRGPLTMNQQAKDAPCWRIYEYVIA
                     SDTLHAVINPATDPDPLTVNDVSPHYLPDGRIVFSTTRQTQSQGILLDEGKPQFAAQD
                     EQRREPAFVLEVMNADGTGVHQISFNQSHDRDATVLANGRVLFTRWDNAPGKDAMSLY
                     SANPDGTDLELYYGANSHLTGTNNTVIEFVHPHPLLDGRIMAIMRQYTDVDDGGALVI
                     IDGEHYVENTQPLLANPQLTGPAQTPATNQDVFTIPGPSPGGRFISAYPLLDGTGRIL
                     VSWSQCRLLDTTQQPPAIVPCTSTALAQPNVQVALPLYSVWLFDPTQNTIQPIMPPVE
                     GTMVTDVAVAQPRPLPTVINDKVPGVGLDENLLNAGVGVIDIRSVYDIDGVDTAVPNI
                     AAVADPVQTPPNTRRARFIRLEKAVSIPDRTVVDLSAAAFGASNYMLEILGYAPIEPD
                     GSVRIEVPGDVAFRMSVLDANARRITPVQGVWLQVLPGEVVNCNGCHLPASAQNPHSH
                     GRQGVFASAWAGASTSGVPFPHTLPTFI"
     gene            2381..3559
                     /locus_tag="VI195_02510"
     CDS             2381..3559
                     /locus_tag="VI195_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823287.1"
                     /translation="MAGALLAGALAAAPAHAGPNEQARRIYERIAGVPPSAAELASMA
                     ATINGGCGGSCAPGAAALVSAAQTATSASSFYSVTLKNMVIPWTNRDQTVFAPFNDYA
                     ATVIGMVRDDVPFNTALSADILYVVNGVSPAPSIANNNHYAAAEANGVDLLANLKKTS
                     QSATYGTPTAATAGLITTRGAESSFFINGTNRAMFRYTMINHFCNDMETLMDTTRPTD
                     RVRQDVARSPGGDSRVFLNTCAGCHSGMDPMAQAFAYYNFNGTPVGDGTNTGTMEYTA
                     GQVQPKYLINSYNFVYGFVTPDDSWSNRWRGGVNASLGWDPTLPGYGNGAKSLGMELE
                     SSDAFAQCQVVKVFTTVCFRAPLATDQPAITSIKASFKSNGYKLKQVFQQAAAACPGQ
                     "
BASE COUNT          595 a         1222 c         1245 g          637 t
ORIGIN      
        1 gtatgaaggt ggggagggtg tgcgggaagg ggactccact cgtgctcgcc ccggcccagg
       61 cggaggcgaa cacgccctga cgcccgtgcg agtgcggatt ctgcgcgctg gcgggcaggt
      121 gacagccatt gcagttgacc acctcacccg gcagcacctg cagccacacg ccctgcaccg
      181 gtgtgatgcg gcgcgcgttg gcatcgagca cgctcatgcg gaaggcgaca tcgcccggca
      241 cctcgatgcg caccgagcca tcgggctcga tcggcgcgta gccgaggatc tccagcatgt
      301 agttgctggc gccgaaggcg gcggccgaga ggtcgacgac ggtccggtcc gggatcgata
      361 ccgccttttc caggcggatg aagcgcgccc ggcgggtgtt gggcggggtc tgcacggggt
      421 cggccacggc ggcgatgttc ggcaccgcgg tgtcgacacc gtcgatgtcg tagacactcc
      481 ttatatctat gacgccgacg ccggcgttga gcagattctc atcgagtccg acgccgggca
      541 ccttgtcgtt gatgacggtc ggcagcggcc gcggctgcgc cacggcgaca tcggtcacca
      601 tcgtgccttc gaccggcggc atgatcggct ggatggtgtt ctgcgtcggg tcgaacagcc
      661 acacgctgta cagcggcagc gcaacttgca cgttgggctg ggcgagcgcc gtcgaggtgc
      721 acggcacgat cgccggcggc tgctgcgtgg tatcgagcag ccggcactgg ctccagctca
      781 ccaggatgcg gccggtgcca tcgagcagtg gataggcgga gatgaagcgt ccgccgggcg
      841 atggccccgg aatggtgaag acgtcctggt tggtggccgg cgtctgcgcg ggaccggtga
      901 gctgcgggtt ggcgagcagc ggctgggtgt tctcgacgta gtgctcgccg tcgatgatca
      961 ccagcgcgcc gccgtcatcg acatcggtgt attgacgcat gatcgccatg atgcggccat
     1021 cgagcagcgg gtgcgggtgc acgaactcga ttacggtgtt gttggtgccg gtcaagtggc
     1081 tgttggcgcc gtagtagagc tcgaggtcgg tgccgtccgg gttggccgaa tagagcgaca
     1141 tggcgtcctt gcccggggca ttgtcccagc gggtgaacag cacgcggccg ttggcgagca
     1201 ccgtggcgtc gcggtcgtgg ctctggttga agctgatctg gtgcaccccg gtaccgtcgg
     1261 cgttcatcac ctcgagcacg aacgccggct cccgacgctg ctcgtcctgc gcggcgaact
     1321 gcggcttgcc ctcatcgagc aggatgccct gcgactgcgt ctggcgagtg gtggagaaca
     1381 cgatgcgccc atccggcagg tagtgcggcg agacgtcgtt gaccgtcagg gggtccggat
     1441 ctgtcgccgg gttgatcacc gcatgcagcg tgtcgctcgc gatcacgtac tcgtagatgc
     1501 gccagcaggg cgcatccttc gcttgctgat tcatggtgag cggcccgcgc atggcgaaga
     1561 tcacgcggct gccgtcggcc gaggtatcga cgtccttcac gtcccacatc tgaccggcgg
     1621 tgatgcgcgc gctgatgttg atttcggccg catccggtga ggcgctcttg cgcatgtaca
     1681 ggtccgccga aggcgtcgac ggcgtgagcg tgcgcatgat gcgcagatcg tcctgcacca
     1741 gcagacccgc ctggtctacc ggcacctgac gcttcacgta gaacaccggg tagtcgacgg
     1801 tcgcgggatc ggtgacctgg ctgttgctgc cgatgtgcca gccgccgctg caggcggcga
     1861 gcgccgccag caccgcgggg aacgcggtgg cgcgcatggc aagcgtgcgg gcgctgcgac
     1921 cccgtcgctt cagctcacgc cagctcggcg agctcatctc cctctcctcg tgccacgcgg
     1981 cccggcggcc ttggctacca atgtaacgta attcatgggt ctgccgatga ccattgccct
     2041 gctgttcgtc atagcgccga ttgcgtcacg gcgcgcccgg cagcacgcgg ttcttatacc
     2101 accgccaggg tgcacgaccg agcgcactac taggtagtcg ttcgaccatg cccacgctgt
     2161 gaaccggctc gcagtttgtt gtgcattgag cggtccaaca caacaacaac acaaactgtc
     2221 tgtgcctggc gacgtcaggc ggttgagaca ttcggggtgg gcttgcgcgc gccttatagt
     2281 tcagctcgcg cattcggtcg ttgtctggga gttgacgagc gccatgaatg tcagaatcaa
     2341 ccgggctctt gttagttttg cccgcggacg tcgcgccgcg gtggcaggcg cgctcctggc
     2401 cggggcgctc gcggcagcgc ccgcgcacgc cggtcccaac gagcaggcgc gccgcatcta
     2461 tgagcgcatc gccggcgtgc cgccctcggc ggcggagctc gccagcatgg ccgccaccat
     2521 caacggcggc tgcggcggca gttgcgcgcc cggcgcggca gcgctggtga gcgcggcgca
     2581 gaccgctacc agcgcgtcga gcttctacag cgtgaccctg aagaacatgg tcatcccctg
     2641 gaccaaccgc gatcagaccg tgttcgcgcc gttcaacgac tatgccgcga ccgtgatcgg
     2701 catggtgcgc gatgacgtgc cgttcaacac cgcgctgtcg gccgacatcc tctatgtcgt
     2761 gaatggcgtg tcgccggcgc cctccatcgc caacaacaat cactacgccg ccgcggaagc
     2821 caacggcgtc gatctgctgg cgaatctcaa gaagacgagc cagtccgcca cctacggcac
     2881 cccgaccgcg gctaccgcgg gactcatcac cacgcgtggc gccgagtcgt cgttcttcat
     2941 caacggcacc aatcgcgcca tgttccgcta caccatgatc aaccacttct gcaacgacat
     3001 ggagacgctg atggacacca cgcgtcccac cgatcgtgtg cgtcaggacg tggcgcgctc
     3061 gcccggcgga gacagccgcg tgttcctcaa cacctgtgcc ggctgccaca gcggcatgga
     3121 tccgatggca caggcattcg cctactacaa cttcaacggc accccggtcg gggacggcac
     3181 caacaccggc acgatggagt acaccgccgg acaggtgcag ccgaagtacc tgatcaactc
     3241 ctacaacttc gtctacggct tcgtcacacc cgatgacagc tggtcgaacc gctggcgcgg
     3301 cggcgtgaac gcctcgctcg gctgggatcc gacgctgccg ggttacggca acggtgccaa
     3361 gtccctcggc atggagctcg agtcgagcga tgcgttcgcg cagtgccagg tggtcaaggt
     3421 attcaccacg gtgtgcttcc gtgcgccgct ggcgaccgat cagccggcga tcacttccat
     3481 caaagcgagc ttcaagtcca acggctacaa gctcaagcag gtgttccagc aagccgcggc
     3541 cgcctgcccg ggccagtaag ggagccgaag ccatgaaccg cgattctctt gcccccctca
     3601 ccgtagccgt cgcgcccgcg cgccgcgcgc tcggcggcgg cgcggtgctg acgctgcttg
     3661 ccgtgctgag cgcctgcggt ggcggcggcg cgcccacca
//
LOCUS       DATDMP010000152         3883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_246221, whole genome shotgun sequence.
ACCESSION   DATDMP010000152 DATDMP010000000
VERSION     DATDMP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246221"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..797)
                     /locus_tag="VI195_02515"
     CDS             complement(177..797)
                     /locus_tag="VI195_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823288.1"
                     /translation="MTPYLKFVSQIVNQSIFVFLMVGAAFALLVGLVLLFDSERAFRI
                     GERMDRWVSTRAAVKPLEEFRSISRPLYRMHRLVGALICAGALYSLVVLGLPSGGDAI
                     NRSLRGIGPAYFSAWISESLRYVLLAGNFGALLFGLVFIVRPSALKSLEAWADRRISE
                     RKAVMPLEKMRMSADQFLRRHPKVVGGLVIAGSLYVLLNLGYALLR"
     gene            953..2656
                     /locus_tag="VI195_02520"
     CDS             953..2656
                     /locus_tag="VI195_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY6823289.1"
                     /translation="MGRPEKIRLGDILVGQKMVSQENLGVALAQQKRSGRKLGRVLVE
                     LGFVTDDQICEAIARQLNIPFVNLKFFNTSQEVVRRLPETQARRFRAIVLEDRAGKYL
                     VGMSDPTDLFAFDELARILKREIQIAVVNDGLLIQTIDRVYRRTEEISGLAKELERDM
                     GDYIDFGALGAGLGAEDAPVVKLLQSVFEDAAQVNASDVHIEPQERAVHIRFRIDGEL
                     LPQTETDVKAGPAIVLRLKLMAGLDISEKRLPQDGRFAIRVRDLPIDVRMSTMPTQHG
                     ESVVLRLLNQKSGLLGLDRVGMPPAMLARFRELIRRTNAMVLVTGPTGSGKTTTLYAA
                     LSELNSVDMKIITVEDPVEYRLPGINQVQINEKIDLTFARVLRSALRQDPDIILIGEM
                     RDQETAQIGLRAALTGHLVLSTLHTKDAISTPIRLIDMGAPNYMVASSVHAVLAQRLV
                     RLVCVSCAEPHPPTDMEQRWLAVHMDPQSLGTARFMHGRGCSNCNGTGFQGRIGVYEL
                     LEMHLDLVRAANKNDANLFTEIARKQMEGQTLGRNAFSLAAEGRTTLAEAIRVASAQE
                     D"
     gene            2657..>3883
                     /locus_tag="VI195_02525"
     CDS             2657..>3883
                     /locus_tag="VI195_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6823290.1"
                     /translation="MPYFNFKGRNSRGELITGTLEGADAGVIADQLMNTGVTPVDISA
                     SQRLAAAEMPQWWRRLNRRKVSLLDLMLFSRQMYTLLKAGVPILKALAGLQESAVNPT
                     MIEVIKDIRVSLDAGRELSASMRRHPDVFSFFYVSLVRVGELTGQLENVFLRLYTHLE
                     FEKEIRESIKAAVRYPIFVLCAMAGAVVIVNLFVIPAFAKVYAGFKAELPFLTKLLIG
                     FSNFMVDTWWLQLAALIGAAFAFRAWIATRAGKYTWDRYKLKVPIAGKIVLKATLARF
                     ARSFSLALQSGVPVVQALSTVAQVVDNDYIGQRIEQMRDGVERGESVLRTAVAAGIFT
                     TVVLQMIAVGDETGELDSLLREVADLYENDVQYEVKTLSQQIEPILIVGLGGLVLVLA
                     LGVFLPIWDLGKAALGK"
BASE COUNT          701 a         1291 c         1278 g          613 t
ORIGIN      
        1 atttgatgca ccgcagcagt cgatgcaaat gaaggctaaa cgctgtggtc agaattcaag
       61 aacgcgccct ccgcggtctc ctccctcctc ctccgcggta aggggttcgt cccctccgca
      121 ctggccgaaa ggcggtgcgg agtgggaccg cgtctgacca gccggtcaga ccttcttcag
      181 cgcaacagtg cgtagcccag attgagcagc acgtagaggc tgcccgcgat caccagcccg
      241 ccgacgactt tcgggtgccg gcgcaggaac tggtcggccg acatgcgcat cttctcgagc
      301 ggcatgaccg ccttgcgctc cgagatgcgc cgatccgccc aggcctccag cgacttgaga
      361 gcgctcggac gcacgatgaa caccagcccg aacagcaggg cgccgaaatt gccggcaaga
      421 agaacatacc gcaggctctc ggatatccag gccgagaagt aggccgggcc gatgcccctg
      481 agggacctgt tgattgcatc tccgcccgag ggcaaaccca gcacgaccag cgagtacagc
      541 gcgcccgcgc agatcaatgc gcccaccagt cggtgcatgc ggtagagcgg ccggctgatg
      601 ctgcggaatt cctccagcgg cttgaccgcg gcgcgcgtcg atacccagcg gtccatccgc
      661 tcgccgattc ggaaggcccg ctcgctgtcg aacagcagca cgagcccgac cagcagcgcg
      721 aacgccgcgc cgaccatcag aaagacgaag atcgactggt tgacgatctg cgagacgaac
      781 ttgaggtagg gcgtcatggc gcggctaggc gcggattcta cttggttgca ctcgctaccg
      841 cgcgcggcga gtcgcgcgcg cgaatgcaaa agaacgtccg gaatgcccga tccctgcggg
      901 aaagccgcaa aagcggataa tgtgcgccgc acgcatcgcg cacggagcag gcatgggacg
      961 ccctgagaag atcaggctcg gcgacatcct cgtcggacag aagatggtct cgcaggagaa
     1021 cctcggggtt gcgctcgcgc agcagaagcg cagcggccgc aagctcggcc gcgtgctggt
     1081 cgagctcggc ttcgtcaccg acgaccagat ctgcgaggcg atcgcgcgcc agctgaacat
     1141 cccgttcgtc aacctgaagt tcttcaacac ctcgcaggaa gtcgtgcggc ggctgcccga
     1201 gacgcaggcg cgccgattcc gcgccatcgt gctcgaggat cgcgccggca agtacctggt
     1261 cggcatgtcg gatccaaccg acctgttcgc gttcgacgag ctcgcgcgca tcctcaagcg
     1321 cgaaatccag attgcggtcg tgaacgacgg cctgctgatc cagaccatcg accgcgtcta
     1381 ccgccgcacc gaggagatct ccgggctcgc caaggagctc gagcgcgaca tgggcgacta
     1441 catcgacttc ggtgcgctcg gggccggtct cggcgccgag gacgcgccgg tcgtgaagct
     1501 gctgcagtcg gtgttcgagg acgcggccca ggtgaacgct tcggacgtgc acatcgagcc
     1561 gcaggagcgt gcggtgcaca tccgcttccg catcgatggc gagctgctgc cccagaccga
     1621 gaccgacgta aaagccggcc cggcgatcgt gctgcgcctg aagctcatgg ccgggctgga
     1681 catctccgag aagcggctgc cgcaggacgg ccgcttcgca atccgcgtgc gcgacctgcc
     1741 gatcgacgtg cgcatgtcga cgatgccgac gcagcacggc gaatccgtgg tgctgcgcct
     1801 gctcaaccag aagtccggcc tgctcggcct ggaccgcgtc ggcatgccgc cggcgatgct
     1861 cgcgcgcttc cgcgagctga tccgccgcac caacgcgatg gtgctggtca ccgggccgac
     1921 cggcagcggc aagacgacga cgctgtacgc ggcgctgtcg gagctgaatt ccgtcgacat
     1981 gaagatcatc acggtcgagg acccggtcga gtaccgcctg ccgggcatca accaggtgca
     2041 gatcaacgag aagatcgacc tgacgttcgc gcgcgtgctc cggtcggcgc tgcgccagga
     2101 ccccgacatc atcctgatcg gcgagatgcg cgaccaggaa accgcgcaga tcgggctgcg
     2161 cgccgcgctt acgggccacc tggtgctctc gacgctgcac accaaggatg caatcagcac
     2221 gccgatccgc ctcatcgaca tgggcgcgcc gaactacatg gtggccagct cggtgcacgc
     2281 ggtgctcgcg cagcgcctgg tgcgcctggt ctgcgtgagc tgcgccgagc cgcacccgcc
     2341 cacggacatg gagcagcgct ggctagcggt gcacatggac ccgcagagcc tgggcacggc
     2401 ccgcttcatg cacggccgcg gctgctcgaa ctgcaacggt accggattcc agggccgcat
     2461 cggcgtctac gagctgctcg aaatgcacct cgacctcgtg cgcgcggcca acaagaacga
     2521 cgccaacctg ttcaccgaga tcgcccgcaa gcagatggag ggtcagacgc tcggccgcaa
     2581 cgcgttcagc ctggcggccg agggccgcac gacgctcgcc gaggcgatcc gcgtggcgag
     2641 cgcgcaggag gactgattgc cgtatttcaa tttcaagggc cgtaactcgc gcggtgagct
     2701 gatcaccggg acccttgaag gcgccgatgc cggcgtgatc gccgaccagc tcatgaacac
     2761 cggcgtcacg ccggtcgaca tcagcgcgag ccaacgcctc gccgccgccg agatgccgca
     2821 gtggtggcgg cggctgaacc ggcgcaaggt gagcctgctc gacctgatgc tgttcagccg
     2881 gcagatgtac acgctgctca aggccggcgt gccgatcctc aaggcgctcg ccggcctgca
     2941 ggaatcggcg gtcaatccca cgatgatcga agtgatcaag gacatccgcg tcagcctgga
     3001 cgctggccgc gagctctcgg cctcgatgcg ccggcacccg gatgtgtttt cattcttcta
     3061 tgtgagcctg gtgcgcgtgg gcgagctcac cgggcagctc gagaacgtgt tcctgcgcct
     3121 ctacactcat ctcgaattcg agaaggagat ccgcgaaagc atcaaggccg cggtgcgcta
     3181 tccgatcttc gtgctgtgcg cgatggcggg cgcggtcgtg atcgtgaatc tgttcgtgat
     3241 ccccgcgttc gccaaggtct atgccggttt caaggccgag ctgccgttcc tcaccaagct
     3301 gctgatcggt ttttccaact tcatggtcga cacctggtgg ctgcagctcg cggcgctgat
     3361 cggcgccgcc ttcgcgtttc gcgcctggat cgcgacgcgc gcgggcaagt acacctggga
     3421 ccgctacaag ctcaaggttc cgatcgcggg caagatcgtg ctgaaggcga cgctggcgcg
     3481 ctttgcgcgc agcttctcgc tggccctgca gagcggcgtt ccggtcgtgc aagcgctcag
     3541 cacggtagcg caagtcgtgg acaacgacta cattggccag cgcatcgagc agatgcgcga
     3601 tggcgtggag cgcggcgaaa gcgtgctgcg cacggccgtg gccgcgggca tcttcaccac
     3661 ggtggtgctg cagatgatcg ccgtgggcga cgagaccggc gagctcgaca gcctgctgcg
     3721 cgaggtcgcg gacctctacg agaacgacgt ccagtacgag gtgaagacac tttcgcagca
     3781 gatcgagccg atcctcatcg tcggtctcgg cggcctggtg ctggtgctcg cgctgggcgt
     3841 gttcctgccg atctgggacc tcggcaaggc tgcgctcggc aag
//
LOCUS       DATDMP010000153         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1023571, whole genome shotgun sequence.
ACCESSION   DATDMP010000153 DATDMP010000000
VERSION     DATDMP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023571"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..501)
                     /locus_tag="VI195_02530"
     CDS             complement(73..501)
                     /locus_tag="VI195_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823291.1"
                     /translation="MIGFLPGTLELFGIVYFRFRLVPRLWCVWLVGVNAACLLFITHI
                     EAQAVLAVTVAAVAAQTLVYQRIGFTRILGTAHILWVPLFAWMATRVDRIVSEPGLAE
                     WLLVLLVTNLVSLVVDAIDAIRFLRGERAPHYRWKSPGLA"
     gene            complement(657..1556)
                     /locus_tag="VI195_02535"
     CDS             complement(657..1556)
                     /locus_tag="VI195_02535"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY6823292.1"
                     /translation="MAASVWPVTDRFVAEIGDVDLSRPLSGPDFRIVEDAFERYAVLV
                     FPDQVLSQQQHIAFAQRFGPIDRSMIVEVEGAKPRVPGEIADVSNLDSQGEILAPGNR
                     LRLFQLGNQLWHTDSAFKHVPAKASLLYQRSIAQTGGQTEFADLRAAWDALPEAMQRK
                     IEGLVAEHAIAYSRAKLGFAMTESEQRALPPVRQALVRHHAASGRTSLYLAAHAGRIV
                     GMAEPAGKALLAELMDFATQRQFVHAHRWRTNDVVMWDNRCTMHRGRPFDDQRWKRDA
                     QRATVEDVANTLEQEGIRVQAVG"
     gene            complement(1582..>2431)
                     /locus_tag="VI195_02540"
     CDS             complement(1582..>2431)
                     /locus_tag="VI195_02540"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEY6823293.1"
                     /translation="PGERPGRLRGAWLLARQSWRVLMLDKELLVFPLLSGFACLLVLA
                     SFVAGVFATGNARSDKTHEDLMLWVLMFVYYFLNYFVIVYFNSALVACAMLRFQGGDP
                     TLAVGLRAANARLPQILAWVLLAATVGVVLRMLQERFALLGRVVIALLGAAWAIATYF
                     IVPVLVVEKRGPFDAVKRSVGLMQQTWGESLVSHVGIGAATGLITFLGVLLIGGASVA
                     LAVATSSLGVLAAGGVVLVAFLVLAALISSALTTIVLSALYLYAAQKKVPQAFEGVAQ
                     YAFAPK"
BASE COUNT          447 a          837 c          806 g          341 t
ORIGIN      
        1 cccgggaaaa tccgatcttc ggttcgaacg gtgacccgga agatatgagc ttgcgcatgg
       61 ttgcaccacc catcaggcaa ggcccggcga tttccaccgg tagtgcggcg cccgctcgcc
      121 gcgcaggaag cgaatggcat cgatcgcatc gacgacgagc gaaaccaggt tcgtgaccag
      181 caatacgagc agccactcgg ccaggccggg ctcgctcacg attctatcga cacgcgtggc
      241 catccatgca aacagcggaa cccacaggat atgggccgta cccaggatcc gggtaaagcc
      301 gatgcgctga tagaccagcg tctgcgccgc gaccgcggcc acggtcaccg ccagaacggc
      361 ctgtgcctcg atgtgcgtga tgaacaacag acaggccgca ttgacgccca cgagccacac
      421 gcaccacagg cgaggcacca ggcgaaagcg gaaatagacg atgccaaaga gctcgagcgt
      481 gccaggcaga aagccgatca ttcttgtctc catcgccatg cgtggagtcc atgctcgcag
      541 cgccgggtgt ctgccggatt tccggcgatg cacggcttgt agcgcgattg tatcaatacg
      601 tacgctcccc atgccccgcc ggccgatctt gcccggagga ccggagcggg cgcgcgctag
      661 ccgaccgcct gcacgcggat gccttcctgc tccagcgtgt tcgccacgtc ctcgacggtc
      721 gcgcgctgcg cgtcgcgctt ccagcgctgg tcgtcgaacg ggcgcccgcg gtgcatggtg
      781 cagcggttgt cccacatgac gacgtcgttg gtgcgccagc ggtgcgcgtg cacgaactgg
      841 cgctgcgtcg cgaaatccat cagctccgcg agcagcgcct tgcccgccgg ctccgccatg
      901 ccgacgatgc ggccggcatg cgcggcgaga tacaggctcg tgcgcccgga agccgcgtga
      961 tggcgcacca gcgcctgccg cacaggcggc agcgcgcgct gctcgctctc ggtcatcgcg
     1021 aagccgagct tggcgcgcga ataggcgatc gcatgctcgg ccaccaggcc ttcgatcttg
     1081 cgctgcatcg cctcgggcag cgcgtcccag gcggcgcgca ggtcggcgaa ctcggtctgc
     1141 ccgcccgtct gcgcgatgga gcgctggtag agcagcgagg ctttcgccgg cacgtgcttg
     1201 aaggcgctgt cggtgtgcca gagctggttg ccgagctgga acaggcgcag gcggttgccg
     1261 ggggcgagga tctcgccctg gctgtcgagg ttggagacgt cggcgatttc gcccggcacg
     1321 cgcggcttgg cgccctccac ctcgacgatc atcgagcggt cgatcggccc gaagcgctgc
     1381 gcgaacgcga tatgctgctg ctggctgagg acctggtccg gaaagaccag caccgcgtag
     1441 cgctcgaacg cgtcctcgac gatgcggaag tccgggccgg aaagcggccg agagagatcg
     1501 acgtcgccga tctcggcgac gaagcggtcg gtgaccggcc agacgctcgc agccacggct
     1561 aacctccttc cctgcggcaa gtcatttcgg cgcgaacgcg tattgcgcca cgccttcgaa
     1621 agcctgcggc accttcttct gcgcggcata gaggtacagc gcgctcagca cgatggtggt
     1681 gagcgcgctg ctgatgagcg ccgcgagcac cagaaaggcg accagcacga cgccgcccgc
     1741 ggccagcacg cccaggctgg aggtcgccac ggccagcgcg acgctcgcgc cgccgatgag
     1801 cagcaccccc agaaaagtga tcaggccggt cgccgcgccg atgccgacat ggctcaccag
     1861 ggactcgccc caggtctgct gcatgagtcc gaccgaacgc ttgacggcgt cgaacggccc
     1921 acgcttctcc accaccagca ccggcacgat gaagtaggtg gcgatcgccc aggccgcgcc
     1981 gagcagcgcg atcacgacgc ggccgagcag cgcgaagcgc tcctgcagca tacgcaggac
     2041 gacgcctacc gtcgccgcca gcagcaccca ggcgagaatc tgcggcaggc gtgcgttggc
     2101 tgcgcgcaag ccgacagcga gcgtagggtc gccgccctgg aagcggagca tggcgcaggc
     2161 caccagcgcc gagttgaagt aaacgatgac gaaataattg aggaagtagt acacgaacat
     2221 cagcacccag agcatgaggt cctcgtgcgt cttgtcgctc cgcgcgttgc cggtggcgaa
     2281 gacgccggcg acgaacgacg ccagcaccag caggcacgcg aacccgctca gcagcgggaa
     2341 gacgagcagc tccttgtcca gcatgagcac gcgccagctc tgccttgcca gcagccacgc
     2401 gccgcgcaac cgtcccggcc gttccccggg a
//
LOCUS       DATDMP010000154         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2135122, whole genome shotgun sequence.
ACCESSION   DATDMP010000154 DATDMP010000000
VERSION     DATDMP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135122"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="pqqC"
                     /locus_tag="VI195_02545"
     CDS             <1..522
                     /gene="pqqC"
                     /locus_tag="VI195_02545"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY6823294.1"
                     /translation="DAAILANCPERAVRRNWVQRILDHDGYGDDPGGIESWLRLAEAV
                     GLERAQVESLQQVLPGVRFAVDAYVNFARRAPWPEAVCASLTELFAPEIHRQRLASWP
                     QHYPWIDPAGLSYFQSRVSLARRDVEFGLALTLERFDTRPAQERALQVLQFKLDVLWQ
                     MNDAMALAYGVTQ"
     gene            519..797
                     /gene="pqqD"
                     /locus_tag="VI195_02550"
     CDS             519..797
                     /gene="pqqD"
                     /locus_tag="VI195_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY6823295.1"
                     /translation="MSDLADTVKPAIAHGLRLQWEAAQDAHVLLYPEGMVKLNGSAGA
                     IMSRCDGVRTLAEIVADLERSYGLTGLATDVRAFVALALQKRWLELRE"
     gene            794..1975
                     /gene="pqqE"
                     /locus_tag="VI195_02555"
     CDS             794..1975
                     /gene="pqqE"
                     /locus_tag="VI195_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY6823296.1"
                     /translation="MIAATPGAAPPPAPAVGPPLWLLLELTYRCPLHCVFCYNPTDFA
                     RTGAELPTADWLRVLREARALGAVQLGLSGGEPLVREDLEAIVAEAHALGFYINLITS
                     GVGLTRARIAALKAAGLDHIQLSFQDSSREMNDFLSSTRTFELKAQVAALIREFGYPM
                     VLNVVLHRLNIDHVGEILEMAEALGAQYVELANTQYYGWAWLNREQLLPSRAQLERAE
                     ATTQRFRARVAGRMQIYFVVPDYFETRPKPCMNGLGSVFLAIAPDGTAMPCHAARMLP
                     GLELPNVRAADVRSIWYESAAFNRFRGEAWMKEPCRSCPERSKDFGGCRCQAYLLTGD
                     PDNTDPVCELSPQHALVTDAVARAERGAQSPRGVREQVLVFRDHRTSLPVVTEDGGGH
                     R"
BASE COUNT          295 a          690 c          698 g          326 t
ORIGIN      
        1 gacgcggcca tcctcgccaa ctgcccggaa cgcgcggtgc gccgcaactg ggtgcagcgc
       61 attctcgatc atgacggcta cggcgatgat cccggcggca tcgagtcctg gctgcggctc
      121 gccgaggcgg tcgggctcga gcgcgcgcag gtggagagcc tgcagcaggt gctgccgggg
      181 gtgcgcttcg cggtcgatgc gtacgtgaac ttcgcgcggc gcgcgccctg gcccgaggcg
      241 gtgtgtgcct cgctcacgga gctgttcgct ccggagatcc accggcaacg tctcgccagc
      301 tggccgcagc actatccctg gatcgacccg gccggcttga gctatttcca gagccgcgtg
      361 agcctcgcgc gccgcgatgt cgagttcgga ctcgcgctca cgctcgagcg cttcgatacg
      421 cgccccgcgc aggagcgggc gctgcaggtg ctgcagttca agctcgatgt gctctggcag
      481 atgaacgacg ccatggcgct ggcgtatgga gtgacgcagt gagcgatctg gccgacacgg
      541 tgaagcccgc cattgcccac ggcctgaggc tgcaatggga ggcggctcag gacgcgcacg
      601 tgctgttgta tccggagggc atggtgaagc tcaacggcag tgccggcgcc atcatgagcc
      661 gttgcgacgg agtgcgtacg ctcgcggaga tcgtcgccga tctcgagcgc agctatggac
      721 tcactggcct cgccaccgac gtgcgcgcct tcgtagcgct ggcgctgcag aagcgctggc
      781 tggagttgcg cgagtgattg ccgccacgcc gggagccgcg ccgccgccag cgcccgcagt
      841 cggtccgccg ctgtggctgc tgctggagct gacttaccgc tgccccttgc actgcgtgtt
      901 ctgctacaac ccgaccgact tcgcccgcac cggtgccgag ctgccgacgg ctgactggct
      961 gcgcgtgctg cgcgaggcgc gcgcgctcgg agcggtgcag ctcgggctct cgggcgggga
     1021 gccgctggtg cgtgaggacc tcgaggcgat cgtcgcggag gcgcatgcgc tcggcttcta
     1081 catcaatctc atcacctcgg gggtcgggct gacgcgcgcg cgcatcgccg cactcaaggc
     1141 ggccggtctc gatcacattc agctctcctt ccaggacagt agccgcgaga tgaacgactt
     1201 cctcagcagc acccgcacct tcgagctgaa ggcgcaggta gcggcgctga tccgcgagtt
     1261 cggctacccc atggtgctga acgtggtgct gcaccggctc aacatcgacc acgtcggaga
     1321 gattctcgag atggcggagg cgctcggcgc acagtacgtc gagctcgcca acacgcagta
     1381 ctacggctgg gcctggctca accgcgagca actgctgccg agccgcgcgc agctcgagcg
     1441 cgccgaagcg accacgcagc gctttcgcgc gcgggtggcc ggccgcatgc agatctactt
     1501 cgtggtgccc gactacttcg agacccgtcc caagccctgc atgaacggac tcggatcggt
     1561 gtttctcgcc atcgcgcccg atgggaccgc aatgccctgc catgcggcgc gcatgctgcc
     1621 cgggctcgag ctgccgaatg tgcgcgctgc ggacgtgcgc agcatctggt acgagtccgc
     1681 ggcgttcaat cgctttcgcg gcgaggcctg gatgaaggag ccctgccgca gctgtcccga
     1741 gcgctccaag gacttcggcg gttgccgctg ccaggcgtat ctcctcaccg gggaccccga
     1801 caacaccgac ccggtctgcg agctctcgcc gcagcacgcg ctggtgacgg atgccgtggc
     1861 acgggccgag cgcggcgcgc agtcaccgcg cggcgtgcgc gagcaggtgc tggttttccg
     1921 cgaccaccgc acctcgctac cggtggtcac ggaagatggc ggcggccacc gctagcgcga
     1981 ggtattctcg gcggccatgg cgatccgcc
//
LOCUS       DATDMP010000155         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1801393, whole genome shotgun sequence.
ACCESSION   DATDMP010000155 DATDMP010000000
VERSION     DATDMP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801393"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VI195_02560"
     CDS             complement(<1..468)
                     /locus_tag="VI195_02560"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6823297.1"
                     /translation="MKESPRRGARLIPVSEAERAARRRLAACYRVFDYLGWTESIFNH
                     ITLRVPGPETIFLINPFGLHYSEVTASNLVAVDVEGSPVRPAAHPVNLAGFVTHSAIH
                     GHIAAAHCVMHTHTTAGSAVACSEHGLSHDTFYGAQLHGRVAYHTFEGVTVNAA"
     gene            complement(658..2187)
                     /gene="adh"
                     /locus_tag="VI195_02565"
     CDS             complement(658..2187)
                     /gene="adh"
                     /locus_tag="VI195_02565"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY6823298.1"
                     /translation="MKTAIIEPGTHGRVHVNFKKRYANYIGGKWVPPMKGEYFEDVTP
                     VTGHALCEVPRSCADDVEAALDAAHAAKAKWGRTSAAERAVLLNRIADRLEANLDYLA
                     TVETWDNGKPIRETTAADLPLAIDHFRYFAGCVRSQEGSIAEIDDDTVAYHFHEPLGV
                     VAQIIPWNFPLLMAAWKLAPALAAGNCVVLKPAEQTPVSILAVLELIGDLLPPGVLNV
                     VNGFGVEAGKPLAASKRVAKVAFTGETSTGRLIMQYASQNLIPVTLELGGKSPNIFFA
                     DVCAQDDAFFDKAIEGFVMFALNQGEVCTCPSRALIQESVYERFMERALQRVAGIKQG
                     DPLDPATMIGAQASSEQLQKILSYMDIARQEGAKVLAGGGRARINSGLQDGYYVQPTV
                     LRGNNKMRVFQEEIFGPVVSVTTFGDQAEALEIANDTLYGLGAGVWSRDANTCYRMGR
                     AIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKNLLVSYSPKALGF
                     F"
BASE COUNT          373 a          761 c          755 g          410 t
ORIGIN      
        1 cgccgcattc accgtcacgc cctcgaaggt gtggtaggcg acccgaccgt gaagctgtgc
       61 gccgtagaag gtgtcgtgcg acagcccgtg ctcactgcag gcgaccgcgc tgccggcggt
      121 ggtgtgggtg tgcatcacgc agtgcgcggc ggcgatgtgt ccgtgaatgg cgctgtgggt
      181 cacgaagccg gcgaggttga cgggatgcgc ggcggggcgc accgggctgc cctcgacgtc
      241 gacggcgacg aggttggagg ccgtcacctc gctgtaatgc agcccgaagg gattgatgag
      301 gaagatcgtc tccggcccgg ggacgcgcag cgtgatgtga ttgaagatcg actcggtcca
      361 cccgaggtaa tcgaacaccc ggtagcaggc ggcgagccgc cggcgcgcgg cccgctcagc
      421 ttcgctcacc gggatcagcc gcgcaccgcg gcgcgggctc tccttcatga ccagacccct
      481 ggccctggcg cccgccttca tctgcgtcgg cctcgcacgg cactcatgct gcagagaata
      541 agccttgcgg ggcggcgctg gaaacggccg ggaagcgccc ggcgccgctg cggctcggcg
      601 ggcggccgtg agcgccgccg cacgcggcgc gttgcgtgca gcggcgactc cccggactta
      661 gaagaacccc agcgccttcg gcgagtagct gaccagcagg ttcttggtct gctggtagtg
      721 atcgagcatc atcttgtgtg tttcgcggcc gatgcccgac tgcttgtagc cgccgaatgc
      781 cgcatgcgcc gggtaagcgt gatagcagtt ggtccagaca cgcccggcct gaatcgcgcg
      841 ccccatgcga tagcaggtgt tggcgtcccg tgaccacaca ccggcgccga gaccgtagag
      901 cgtatcgttg gcgatctcga gcgcctcggc ctgatccccg aaggtcgtga ccgacaccac
      961 cggcccgaag atctcctcct gaaagaccct catcttattg ttgccgcgca gcaccgtcgg
     1021 ctgcacgtag taaccgtcct gcaggccgct gttgattcgc gcacgcccgc cgcccgccag
     1081 caccttggca ccctcctggc gtgcgatgtc catatacgag agtatcttct gcagctgctc
     1141 gctcgaggcc tgcgcgccga tcatcgtcgc aggatcgagc ggatcgccct gcttgatgcc
     1201 ggcgacgcgc tgcagcgccc gttccatgaa gcgctcgtag acggactcct gaatgagcgc
     1261 gcgtgacggg caggtgcaca cctcgccctg gttgagcgcg aacatgacga acccttcgat
     1321 cgccttgtcg aaaaaggcgt catcctgcgc acacacgtcg gcgaaaaaga tgttgggcga
     1381 cttgcccccg agctcaagcg tcaccggaat gagattctgc gatgcatact gcatgatgag
     1441 ccgcccggtg gacgtctccc cggtgaaggc gaccttcgcc acccgcttgc tcgcggcgag
     1501 cggcttgccg gcctcgactc cgaagccatt gaccacgttc agcacaccgg gcggcagtaa
     1561 gtcgccgatc agctcgagta ccgccagaat cgacaccggt gtctgctcgg cgggcttcag
     1621 caccacgcag ttgccggctg caagtgccgg cgccagcttc caggcggcca tcagcagcgg
     1681 gaagttccac ggaatgatct gggccacgac gccgagcggc tcgtggaagt gatatgcgac
     1741 ggtgtcgtcg tcgatctcgg cgatcgagcc ttcctgcgag cgcacgcagc cggcgaagta
     1801 gcggaaatga tcgatcgcca gcggcaggtc ggcggccgtc gtttcgcgaa tcggcttgcc
     1861 gttgtcccag gtctcaaccg tggcgaggta gtcgaggttc gcctccagcc ggtcagcgat
     1921 cctgttgagg agcacggcgc gctcggcggc cgaggtcctg ccccactttg ccttggcggc
     1981 atgcgcggca tcgagagccg cctcgacgtc gtcggcacag gagcggggta cttcgcacag
     2041 cgcgtgcccc gtcacgggag tgacgtcttc gaaatactcc cccttcatcg gtggcaccca
     2101 cttgccgccg atgtagtttg catagcgctt cttgaagttc acgtggacgc gcccgtgcgt
     2161 gcccggttcg atgatcgcag tcttcacttg tctccccccg gcgaactgtg cctgctgatg
     2221 cgtggtggcg gttcgcctca cgtcttgtca tgaagtctcc ggcagtcgta gcacgcatcg
     2281 ccatacgtgg aagcactta
//
LOCUS       DATDMP010000156         4843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1912391, whole genome shotgun sequence.
ACCESSION   DATDMP010000156 DATDMP010000000
VERSION     DATDMP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912391"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1508
                     /locus_tag="VI195_02570"
     CDS             324..1508
                     /locus_tag="VI195_02570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY6823299.1"
                     /translation="MLRLKRGEERRLLAGHLWVFSNEVDTERTPLGQFVPGALVELRS
                     YRDSFLAHAYINPHALICARILSRDAAQPVTAELIGQRLTAALALRERVGDARYCRWV
                     FGESDLLPGLVLDRYGEVVVGQIATAGMEALREPIENAVRRVVQPQALIWKNDSAARD
                     LEQLPRVVQPGFGAVPAEVGIVEAGLSFAAPLAQGQKTGWFYDQSANRARLRRYVAAG
                     ARVLDVCSYVGAWAVTALDQGAGRACCVDSSPLALEYAEANARRNGKTIEPLRADAFD
                     ALKTLLAQGEQFDVVVLDPPAFIKRKKDIPQGQAAYRKLNQLALALLADGGLLVSCSC
                     SYHLAAEQLLEAIQAAARHSARFVQILETAGQSPDHPLHPAIPESRYLKAFFCRVTRA
                     VG"
     gene            1521..2336
                     /gene="lgt"
                     /locus_tag="VI195_02575"
     CDS             1521..2336
                     /gene="lgt"
                     /locus_tag="VI195_02575"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY6823300.1"
                     /translation="MLVYPGFNPIAFEVGRVKVHWYGIMYLLGFAAAWWLARRRAARP
                     ASTWRPQDVDDLIFFAMLGVIFGGRIGYVLFYGLTFWASDPWYPLKIWEGGMSFHGGL
                     IGVIVALTLFAWRRGRNAADVYDFTAPLPALGLFFGRIGNFINSELWGKVTTVPWGFQ
                     VNGVVRHPSQLYEAALEGLLLFAVLWWFTSRPRPRLAPSGLFLLIYGLARFLIEFVRV
                     PDEHIGYLAGGWLTEGQVLSMPMILVGIGMLAWAYRARQPSGNFAAAQVTPAS"
     gene            2333..3127
                     /gene="thyA"
                     /locus_tag="VI195_02580"
     CDS             2333..3127
                     /gene="thyA"
                     /locus_tag="VI195_02580"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY6823301.1"
                     /translation="MKQYLELLQRIRTLGARKADRTGTGTLSLFGEQLRFDLGAGFPL
                     VTTKKIHLPSVVYELLWFLRGDSNVAWLHEHGITIWDEWADEHGELGPVYGRQWRAWA
                     TADGRHIDQIAHALEQLRRNPDSRRIMVSAWNVGELERMRLEPCHAFFQLYVAGGRLS
                     CQMYQRSADVFLGVPFNIASYALLTHLFAQQCDLIPGELVWTGGDCHLYLNHLEQADL
                     QLQRTPLPLPRLAIRRRPPSIFEYRYEDFEFHDYQHHAPIRAPVAV"
     gene            3124..3624
                     /locus_tag="VI195_02585"
     CDS             3124..3624
                     /locus_tag="VI195_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY6823302.1"
                     /translation="MSPARVAGVPLIEVRASSLHGLGVFAACRIDRGTRIIEYLGERV
                     SHAEADRRYESKDAGDAHTFLFIVDGRTVIDAGVDGNDARFINHACDPNCESVIEQRR
                     VFIDALRAIEPGEELTYDYQIQREPDDPPDVDAIFACRCGSKGCRGSMLWPPRSRPDR
                     RRALQR"
     gene            3576..>4843
                     /locus_tag="VI195_02590"
     CDS             3576..>4843
                     /locus_tag="VI195_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEY6823303.1"
                     /translation="MAAAQQTGPAARVAALSAVTASPGHMHNSRRLTLSSIVGATGIV
                     YGDIGTSPLYALQQAVGEAGSFDAAAVLGVLSLIFWALAISVTLKYVTIIMRADNEGE
                     GGILALFALAQRRLIYGSTWGKVAVGLALAGTAFFFCDALITPAISVLSAVEGLELLS
                     PDLKQAVVPITLLVLAVLFAYQRHGTARVGSLFGPIMVVWFVAIAAIGIFPILRHPQV
                     LAALNPLEGAGLLARRPEVALAVIGAVFLALTGAEALYADMGHFGRTPVRVSWYTLVL
                     PALVIDYFGQGALLLELGKPVEHPLYQLVPPAVLPWLLVLATAATVIASQAVISGAFS
                     MARQAVHLDLLPRLRVLQTSALEHGQIYVPMVNWLVFIAVCGFVIGFGSSDALSGAYG
                     AAVVGTMVVTTVLGAFVAATQWNWPKWQVGA"
BASE COUNT          710 a         1626 c         1589 g          918 t
ORIGIN      
        1 gtctcggacg ccaaatggtt cgtgtacttt ggcgtgccgt cgctattcaa atgcctcatc
       61 tgcgtcatcg ttgcaagtta acgctttaac gcggagcagc gggcagcaca ccagtagata
      121 cacatttcat cgatagttaa ccaaatggtg aactatcggc caggacagct gtgaaaaatc
      181 tcggcaagca cctggcatgc aaagctgact ctgaagggcc gccgcttgct ttctgtatac
      241 tctgaatcca caccccgcgc atttgaaatg tgatgtctca tcccgcacct tcgctggtcc
      301 aacccgccga tactgatgca cgcgtgctgc gcctcaagcg tggcgaggag cgccgtttac
      361 tcgcgggaca tctgtgggtg ttcagtaacg aggtcgatac cgagcgcaca ccgctcgggc
      421 agttcgttcc cggcgcgctg gtcgagctgc gctcctaccg cgacagcttc ctcgcccacg
      481 cctacatcaa cccgcatgcg ctgatctgcg cgcgcatact gtcgcgcgat gcggcgcagc
      541 cggtgaccgc cgagctcatc gggcaacgtc tgaccgccgc gctggcactg cgcgagcgcg
      601 tgggcgatgc gcgctactgc cgctgggtgt tcggcgagtc ggacctgttg ccgggcctgg
      661 tgctcgatcg gtacggcgag gttgtcgtcg gtcagatcgc caccgccggc atggaggcgc
      721 tgcgcgagcc gatcgagaac gccgtgcgcc gcgtcgtgca gccgcaggcg ctcatctgga
      781 agaacgactc cgccgcgcgt gacctcgagc agctgccacg tgtggtacag ccggggttcg
      841 gcgccgtccc ggccgaggtc ggtatcgtcg aggcggggtt gagcttcgct gcgccgctcg
      901 cgcagggtca gaagaccggt tggttctacg accagagcgc caaccgcgcg cgcctgcggc
      961 gctatgttgc tgcgggcgcg cgcgtactcg atgtgtgctc gtatgtcggc gcctgggcgg
     1021 ttaccgcgct cgatcagggc gccggccgtg cctgctgcgt cgactcatcg ccgctcgcgt
     1081 tggagtacgc cgaggccaac gcgcgccgca acggcaagac catcgagccg ctgcgcgccg
     1141 atgccttcga tgcgctgaag acgctgctgg cgcagggcga gcagttcgat gtagtggtgc
     1201 tcgacccgcc ggcgttcatc aagcgcaaga aggacattcc ccagggccag gccgcctacc
     1261 gcaagctcaa ccagctcgcg ctcgcactgc tcgcggacgg cgggttgctg gtgtcgtgct
     1321 cctgctccta tcacctggcc gccgagcagc tgctcgaggc catccaggcg gcggcacggc
     1381 acagcgcgcg cttcgtgcag atcctcgaga cggccggcca gtcgccggac cacccgctgc
     1441 accctgccat tccggaaagc cgttacctga aagcgttctt ctgccgcgtg acacgcgcgg
     1501 tcggctaaac ttcccgcccc atgctcgtct atcccggatt caacccgata gctttcgagg
     1561 tcggccgcgt caaggttcac tggtacggca tcatgtacct gctgggcttc gcggccgcct
     1621 ggtggctcgc gcggcggcgc gcggcgcgcc cggcctccac ctggcggccg caggatgtcg
     1681 acgacctgat cttcttcgcg atgctgggcg tgatcttcgg cggccgcatc ggttacgttc
     1741 tgttctacgg tctcaccttc tgggcgagcg atccgtggta tccgctgaag atctgggagg
     1801 gcggcatgtc cttccacggc ggcctcatcg gggtgatcgt cgcactcacg ctgttcgcct
     1861 ggcgccgcgg ccgtaacgcc gcggatgtgt acgacttcac cgcaccgctg ccggcgctcg
     1921 ggctgttctt cggccgcatc ggcaacttca tcaacagcga gctgtggggg aaggtgacca
     1981 ccgtgccgtg gggattccag gtgaatggcg tggtgcgcca tccttcccag ctgtacgagg
     2041 cggcgctcga aggcctgctg ctgttcgccg tgttgtggtg gttcacctcg cgaccgcggc
     2101 cgcggctcgc gccctcgggg ttgtttctgc tcatttatgg tctggcgcgc ttcctgatcg
     2161 agttcgtacg tgtgccggac gagcacatcg gctatctcgc cggcggctgg ctgaccgagg
     2221 gacaggtgct gtctatgccg atgatcctgg tcggcatcgg catgctggcc tgggcatatc
     2281 gcgcccgcca accttcgggc aacttcgccg ccgcgcaggt gaccccggca tcatgaagca
     2341 gtatctcgag ctgctgcagc gcatacgcac ccttggtgcg cgcaaggcgg accgcaccgg
     2401 caccggcacg ctgtcgctgt tcggcgagca gctgcgcttc gatctcggcg cgggctttcc
     2461 gctcgtcacc accaagaaga tccatctacc gtcggtggtg tacgagctgc tgtggttcct
     2521 gcgcggcgac agcaacgtgg cctggctgca cgagcacggc atcaccatct gggacgagtg
     2581 ggcggatgag cacggcgagc tcggtccggt atatggacgg cagtggcgcg cctgggccac
     2641 agccgatggc cggcacatcg atcagatcgc gcacgcgctc gagcagctca ggcgcaatcc
     2701 ggattcgcgc cgcatcatgg tgagcgcctg gaacgtgggc gagctcgagc gcatgcggct
     2761 cgagccgtgt cacgcgttct tccagctgta cgtggccggc gggcgcctgt cctgccagat
     2821 gtaccagcgc agcgccgacg tcttcctcgg cgtaccgttc aacatcgcca gctatgcgtt
     2881 gctcacgcac ctgttcgcgc agcagtgcga cctcatcccc ggtgagctcg tctggaccgg
     2941 gggcgactgt cacctgtacc tcaaccatct cgagcaagcg gacctgcaac tgcagcgcac
     3001 cccgctgcct ctgccgcgtc tggcgatccg gcgccggccg ccgagcattt tcgagtaccg
     3061 ctacgaggac ttcgagttcc acgactacca gcaccatgcg ccgatccgcg caccggtggc
     3121 ggtatgagcc ccgcgcgtgt tgccggcgtg ccgctcatcg aggtgcgcgc ctcgtcgctg
     3181 cacggactcg gggtattcgc cgcgtgccgc atcgacaggg gcacgcgtat catcgagtac
     3241 ctcggtgagc gcgtatcgca tgcggaagcc gaccgccgct acgaaagcaa ggatgccggc
     3301 gacgcccaca cctttctttt catcgtcgat gggcgcacgg tcatcgatgc cggggtcgac
     3361 ggcaacgatg cgcgcttcat caatcatgcc tgtgatccga actgcgagtc ggtgatcgag
     3421 cagcgccgcg tgttcatcga cgcgctgcgc gccatcgagc cgggtgagga gctgacctac
     3481 gactatcaga tccagcggga gcccgatgac ccgccggatg tcgatgcgat cttcgcctgc
     3541 cgctgtggca gcaagggctg ccgcggcagc atgctgtggc cgccgcgcag cagaccggac
     3601 cggcggcgcg cgttgcagcg ctgagcgcag tcacggcttc gccgggccac atgcataact
     3661 cgcgccgcct cactctgtcg tccatcgtcg gcgcgaccgg catcgtctat ggcgacatcg
     3721 gcaccagccc gctgtacgcg ctgcagcagg cggtgggcga ggccggtagc ttcgatgccg
     3781 cagcggtgct cggagtgttg tcgctcatct tctgggcact cgccatctcc gtgacgctca
     3841 agtacgtcac catcatcatg cgtgccgata acgaggggga gggcggcatc ctggcgctgt
     3901 tcgcgctggc acagcggcga ctgatctacg gcagtacctg gggcaaggtc gccgtcggac
     3961 ttgcgctcgc gggcacggca ttcttcttct gcgatgcgct catcacgccc gccatctcgg
     4021 tgctgagcgc ggtggaaggg ctggagctgc tcagccccga tctgaagcag gcggtggtgc
     4081 cgatcactct gctagtgctc gcggtgctgt tcgcctacca gcggcacggc accgcgcggg
     4141 tcgggagcct gttcggaccc atcatggtgg tctggttcgt agcgatcgcc gcgatcggaa
     4201 tattccccat cctgcgccat ccacaggtgc tcgcggccct caatccgctc gagggtgccg
     4261 gcctgctcgc gcgccggccg gaggtcgcgc tcgcggtgat cggcgcggta ttcctggcgc
     4321 tcaccggtgc ggaggcactg tacgccgaca tgggacactt cgggcgcacg ccggtacggg
     4381 tctcctggta cacgctggtc ctgcctgccc tggtcatcga ctatttcggc cagggagccc
     4441 tgctgctcga gctggggaaa cccgtcgagc atccgctcta tcaactggtg ccacctgcgg
     4501 tgctgccgtg gctgctcgtg ctcgcgaccg ccgccaccgt cattgcctcg caggcggtca
     4561 tctcgggagc tttctccatg gctcgccagg ccgtgcacct cgatctgcta ccgcgcctgc
     4621 gtgtgctgca gacctcggcg ctcgagcacg gacagatcta cgtgccgatg gtgaactggc
     4681 tggtgttcat cgccgtgtgc ggcttcgtca ttggtttcgg ttcctcggat gcgctctcgg
     4741 gcgcctacgg tgcggcggtc gtcggtacca tggtcgtcac cacggtgctc ggcgccttcg
     4801 tcgccgcgac acagtggaac tggccgaagt ggcaggtcgg cgc
//
LOCUS       DATDMP010000157         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1690563, whole genome shotgun sequence.
ACCESSION   DATDMP010000157 DATDMP010000000
VERSION     DATDMP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690563"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VI195_02595"
     CDS             complement(<1..454)
                     /locus_tag="VI195_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823304.1"
                     /translation="MYEVSATVSHDALVTPGSVYVFAQGDTQKVQLTPNADRSQWRGF
                     YAARCRSSFPLQFVAEWRLPLDVRRKVLPPQPRQVRLLEPPLTRSASFDSSGKAPKGG
                     WQGTVQYSFVTVPSVQITGAHIEPQSASEEDAAAAKPLSVVSSFPLAGG"
     gene            complement(1276..1617)
                     /locus_tag="VI195_02600"
     CDS             complement(1276..1617)
                     /locus_tag="VI195_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEY6823305.1"
                     /translation="MTLRQLLLPYIGALFLATLGPCASRANSGSAQSTIVMAKDFMFA
                     PTSLTVAAGSTVTWTNRDDEPHTVVSESGLFRSAALDTNESFSFRFAQPGTYHYACSI
                     HPRMVGTIIVR"
     gene            complement(1614..>2030)
                     /locus_tag="VI195_02605"
     CDS             complement(1614..>2030)
                     /locus_tag="VI195_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823306.1"
                     /translation="GTLGTEQLAWLAGDLKGRSSSTPIVVFAHMPLWSVYEPWGWGTG
                     DAPQLLEQLRRFGSVTVLNGHIHQIVQKVEGNVTFHTARSTAYPQPVAGEGAGPGPLK
                     VPADQLPGMLGVTRVSVVRHPRSLRLDDTTIAEGAA"
BASE COUNT          378 a          661 c          656 g          335 t
ORIGIN      
        1 agccgcccgc caacgggaat gaggaaacca cggacagagg cttggccgca gcggcatcct
       61 cctcggaagc actctgcggc tcgatgtgcg cgccggtgat ctgcacgctc ggaaccgtga
      121 cgaagctgta ttgcacggtg ccctgccaac cgcccttcgg tgccttgccg gaggagtcga
      181 agctcgccga gcgtgtgagc ggtggctcga gcagacgcac ctggcgcggc tgcggcggta
      241 gcaccttgcg cctgacgtcc aggggcagcc tccactccgc cacgaactgc agcgggaaac
      301 tgctgcggca tcgcgccgcg tagaacccgc gccactgtga gcggtcggca ttgggagtca
      361 actgcacctt ctgcgtatcg ccctgcgcaa acacatatac cgagcccggt gtcaccaacg
      421 catcgtgcga caccgtggct gacacctcgt acatgcccag gtcatggttg gccggatatt
      481 ccgcaggcgt cgtgtcccgt atctgtacgt cgcacgcaca aagcaacagc gcggcagaga
      541 gccacgtgag ccgtatgagc ctgcgcatca gccgattcgc cccaggaacc gttatgttgt
      601 cgtggacgcg agccggcggc cctcgtgggg cacgccgcac ggcgccgtgc tacgcggacc
      661 gaagcgccgc gggaaccgct cgcgagacgg aggcccggac ttacttcttg cggcgcgctc
      721 ccgaccgctt cgctgcacgg aaagaaatcc cggcgcgcat ggccttctgc tgtaccgcag
      781 ccgcggtgcg cccaagcctt ctggcgacct gacttgtcgg ggttcctgac ttcgcgagcg
      841 ccctgagcgc cgccaattca cgggcgctcc aggacgcgcc atcgttcggt ggacgtcgtg
      901 ccattagtct tctccgcaaa acacccaacg cagggtcgga acttccgatc atacactgat
      961 tcgcatgctg tgcgagtggg ttgcaggcat cggttacatg ttggaaatcg ccaacgacca
     1021 ggcgcgggcg acggcctgtg cgcggccaac tcggcacact gtggcttcgc cggctgaaaa
     1081 catctgacac aactacaact tgcaactatc gtgatgtggc gctgccgcaa gcgccgcgtt
     1141 ggaacagttg caaagctcaa actgcgcaag gcctgcggcc gcgcccttgt aaagagcctc
     1201 ccaaggcaca ggcgtcggtt atttccgaca catcggcctg ccgtaacgcg tgtttgatgg
     1261 cccgattgtc aacagctagc gcacgatgat ggttcccacc atgcgcgggt gaatggagca
     1321 ggcgtagtga taggtgccgg gctgcgcgaa gcggaacgag aagctctcgt tggtatcgag
     1381 ggctgcagag cgaaacagtc cactctcgct cactacggtg tgcggctcat cgtccctatt
     1441 tgtccaggta acggtcgagc cggcggcgac ggtgagtgag gtcggcgcga acatgaaatc
     1501 cttcgccatc acgatggtgg actgcgcgga gccggagttg gcacgcgacg cgcatggccc
     1561 gagcgtggcg aggaacaaag cgccgatgta gggcagcagc aactgacgca gagtcatgcc
     1621 gcgccctcag caatcgtcgt atcgtcgagc cgcagcgagc gcggatgcct caccacggat
     1681 acacgcgtga cgccgagcat gcccggcagc tgatcggccg ggaccttcaa cggcccaggc
     1741 ccggcaccct caccggccac cggctgcgga taggcagtcg agcgcgccgt atggaaggtc
     1801 acgttgccct ccaccttctg cacgatctgg tggatgtggc cgttgagcac cgtgaccgag
     1861 ccgaagcggc gcagctgctc gagcagttgc ggcgcatctc ccgttcccca gccccagggc
     1921 tcgtagacgc tccacagcgg catgtgagca aagaccacga tcggggtgct ggaggagcgg
     1981 cccttgaggt caccagcaag ccaggcgagc tgctcggtgc cgagggtgcc
//
LOCUS       DATDMP010000158         6665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_246496, whole genome shotgun sequence.
ACCESSION   DATDMP010000158 DATDMP010000000
VERSION     DATDMP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246496"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VI195_02610"
     CDS             <1..542
                     /locus_tag="VI195_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY6823307.1"
                     /translation="IRDDAGELRARLRFHLETHDVLVLSGGVSMGRFDLVPQVLGELG
                     VRTIFHKVAQRPGKPLWFGVAPSGAAVFGLPGNPVSTLVCLTRYVLPALRGSLGQSPE
                     RLERMALSAPLTVMPPLTHFMPVRLEQDDWGRDWAVPAPTNGSGDFTALAGTEGFVEL
                     PPGPNTYAKGFVTRLYRWS"
     gene            642..1697
                     /gene="moaA"
                     /locus_tag="VI195_02615"
     CDS             642..1697
                     /gene="moaA"
                     /locus_tag="VI195_02615"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY6823308.1"
                     /translation="MSNREVIVPLRAVSAPRDSFSRPMRDLRISVMDRCNFRCPYCMP
                     RETYHERYRFLGTHERLSFDEIVRLARLFARLGVRKLRLTGGEPLLRANLPELIGDLT
                     DVPGVEDVALTTNGVLLSRYAGELKAAGLRRVTVSLDSLDPQVFARMSGGFGGIEEVL
                     EGIEHARRAELTPIKINAVVQRGVNDHTVLELIERFRGTGIIVRFIEYMDVGNRNHWS
                     AAAVVPAKELLAAIGQRWPLHPLDPSYRGEVARRYAFADGQGEVGFISSVSQPFCGDC
                     TRARLSSDGVLYTCLFATHGTSLRDALRGGASDDQLLELIRAVWLGRADRYSEQRASL
                     RSAASDERKVEMFYIGG"
     gene            1697..2197
                     /gene="moaC"
                     /locus_tag="VI195_02620"
     CDS             1697..2197
                     /gene="moaC"
                     /locus_tag="VI195_02620"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY6823309.1"
                     /translation="MARRGLTHLNSRQQPAMVDVGAKQVTHRVAVAEARVRLPLAVAR
                     ALRAAGHRTRKGPVFDTAIVAGVQAAKRAHELIPFCHPLGLENCQLHIEHRRAQEIVV
                     RCEVSVHHRTGVEMEALTGAAVAALTIYDMCKALSHDIEIGSVRLLAKAGGRRRFARS
                     AAERRP"
     gene            2194..3066
                     /locus_tag="VI195_02625"
     CDS             2194..3066
                     /locus_tag="VI195_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY6823310.1"
                     /translation="MNSQPGNGRTGAAPLLGLVLAGGRSTRMRTDKAALLYAGRSQLE
                     RAIELIAPHVERSFVSVRADQSEDALRARFAQIRDVHDNLGPVAGLLAAQARHPEAAW
                     LVLACDLPLLDAATLANLVRCRAPQRAATAYRSSHDGLPEPLCAIWEPRSAAPLAAYV
                     ARGRQCPRRFLLDADVRLLEEPNPEALDNVNTPEEYRSVMSALLPEPTSGPKQITVQY
                     YALLREQAGRRDEALVTSARTAAELYAELARRYPFSLAPEVLRVAINAEFREWTAALA
                     DGDAVVFIPPVAGG"
     gene            3082..3537
                     /locus_tag="VI195_02630"
     CDS             3082..3537
                     /locus_tag="VI195_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY6823311.1"
                     /translation="MAAFRFMRTPITTEELRVALADPACGGYATFEGWVRDRNEGQRV
                     RTLEYEAFEALGVGEGERIIAEARERFGVAHAACVHRIGVLAVGELAVWVGVSAAHRD
                     EAFRACRYIIDEVKHRLPIWKKEHYLNGDSGWVNCERCATAPKHADAQA"
     gene            complement(3646..4887)
                     /locus_tag="VI195_02635"
     CDS             complement(3646..4887)
                     /locus_tag="VI195_02635"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY6823312.1"
                     /translation="MEGFGRTAVAAAQVLALASSTQKDAALAAAAAAIRARTATLLAA
                     NERDMSAARARRLSGPRLERLRLDERRIAAIAAGIDAVLALPDPIGRVAAEWERPNGL
                     RIQRITVPLGVIGIIYESRPNVTADAGALCLKSGNACILRGGSESQYSSHALHECLVS
                     GLAAADLPLGCIQLVPTSDRAAVGYLLAGMGEYLDVIVPRGGRSLVERVQREARVPVI
                     GHLEGNCHVYIDRDADVRMARAIALNAKMRRTGICGAAETLLVDEACAATHLAPVIRD
                     LLAAGCEVRGDPAVQAVDPRVRPASEDDWYTEYLDAIIAARVVAGVDAAIAHIARYGS
                     AHTESIVTENAATAERFMQRVDSAIVLHNASTQFADGGEFGMGAEIGISTDRFHARGP
                     VGVEQLTSYKYLVRGTGQVRP"
     gene            complement(4919..6106)
                     /gene="proB"
                     /locus_tag="VI195_02640"
     CDS             complement(4919..6106)
                     /gene="proB"
                     /locus_tag="VI195_02640"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY6823313.1"
                     /translation="MAGELIVSAVPLAQARRIVVKVGSALLVDAESARVNRTWLETLI
                     EDLLRLRKRGQRVILVSSGAIALGRRRLGLKHGTLRLEESQAAAAVGQIRLAHAYKEL
                     LESNDVTVAQVLLTLEDSERRRRYLNARATLESLLELGALPVINENDTVATAEIRYGD
                     NDRLAARVAQMAGADCLVLLSDVAGLFSADPTRDPSARFIHEVRQITPEIEAMAGRTA
                     SQLGSGGMAAKIAAARIALAAGCHMCIAAGAPRHPLRRIEEGAECTWFIPTATPAAAR
                     KQWIAGTLRPAGAITIDAGALRALLEGRSLLPAGVTGTRGRFDRGDTVSVLAADGVEV
                     ARGIIAYSDSDAARIMGRRSSEIAEILGFRGRDEMIHRDDLVLLRQEYAAAAVAATPQ
                     PAR"
     gene            6202..6522
                     /locus_tag="VI195_02645"
     CDS             6202..6522
                     /locus_tag="VI195_02645"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HEY6823314.1"
                     /translation="MSRDYEPVPGQWYENLEEEESFRVLSVDEDSERLEIEYLDGDIE
                     EIDLETWHEMDLERIEEPEGWSESDDEDAEDDDDDWDEDEEDEDDDDWDEDEDEADDQ
                     RDEY"
BASE COUNT         1055 a         2246 c         2285 g         1079 t
ORIGIN      
        1 acatccgcga tgatgccggt gagctgcgcg cgcgcttgcg ctttcatctc gagacgcacg
       61 atgtgctcgt gctctccggt ggggtgtcga tgggccggtt cgacctggtg ccgcaggtgc
      121 tcggggagct cggtgtacgc acgatcttcc acaaggtagc gcaacgtccg ggcaagcctc
      181 tgtggttcgg tgtcgctccc tccggggcgg cggtgtttgg gctgcccggc aatcccgtct
      241 cgacccttgt gtgcctgacg cgttatgtgt tgccggcgct gcgcggcagc ctcggccaga
      301 gccccgagcg gctggagcgc atggcgctga gtgccccgct caccgttatg ccgccgctga
      361 cgcacttcat gcccgtgcgg ctcgagcagg acgactgggg tcgggactgg gccgttccgg
      421 cgcccaccaa cggctccggc gatttcaccg ctctggcggg gaccgaaggg ttcgtcgagt
      481 tgccgcctgg gccgaacacc tacgccaagg gtttcgtgac ccgcctgtat cgctggtcct
      541 gagggttgca accggcgatc gcacccccac ccatcgcggg taccgccgga ggcgcgggca
      601 attggccttt ggctcgagcg gtgctcatac tgtcgtgacc catgtccaat cgtgaagtga
      661 tcgtacccct gcgggccgtg agcgcgcccc gggacagctt ttcccggccg atgcgcgacc
      721 tgcgcatctc ggtcatggac cgctgcaatt tccgctgccc ctattgcatg ccgcgcgaga
      781 cctaccacga gcgttaccgc ttccttggca cgcatgagcg tctgtcgttc gacgagatcg
      841 tgagactcgc acgactgttc gcgcggctgg gagtgcgcaa gctgcggctc accggcggcg
      901 agccgctgtt gcgcgcaaac ctgccggagc tgatcgggga cctcaccgat gtcccgggag
      961 tcgaagatgt cgcgctcacc accaatggcg tgctgctgtc gcgctacgcc ggtgagctca
     1021 aggccgccgg acttcggcgc gtgacggtca gtctcgatag cctcgatccc caggtgttcg
     1081 cgcgcatgag tggcggcttc ggcggcatcg aggaagtgct cgagggcatc gagcatgcac
     1141 ggcgcgccga gctgacgccg atcaagatca acgcggtggt gcagcgcggt gtcaacgatc
     1201 atacggtgct cgagctcatc gagcgctttc gtggcaccgg gatcatcgtg cgcttcatcg
     1261 agtacatgga cgtgggcaac cgcaaccact ggagcgccgc ggcggtggtg cctgcgaagg
     1321 agctgctggc ggccatcggc cagcgctggc cactgcatcc tctcgatccc agctatcgcg
     1381 gcgaggtcgc gcgtcgctac gcattcgccg atggacaggg ggaggtcggc ttcatttctt
     1441 cggtatcgca gccgttctgc ggcgactgta cgcgcgcacg gctgtcgtct gatggcgtgc
     1501 tctacacctg cctgttcgcc actcacggca ccagcctgcg cgatgcgctg cgcggcggcg
     1561 cgagcgacga tcagctgctc gagctcatcc gtgctgtctg gctcggccgt gccgaccgtt
     1621 acagcgagca gcgcgccagc ctgcggagcg ccgcgagcga tgagcgcaag gtcgagatgt
     1681 tctatatcgg tggctgatgg cacgtcgcgg cctcacacat ctcaactcgc gtcagcagcc
     1741 tgcgatggtg gacgtcggag cgaaacaggt gacgcatcgc gttgccgtcg ctgaggcacg
     1801 ggtgcgcttg ccgctcgctg tggcacgtgc cctgcgggcc gccggacatc gcacccgcaa
     1861 aggcccggtg ttcgacaccg caatcgtcgc cggggtgcag gcggccaagc gcgcgcacga
     1921 gctcatcccc ttctgccatc cactgggact cgagaactgt cagctgcaca tcgagcaccg
     1981 ccgcgcgcag gagatcgtgg tgcgctgcga ggtgtcggtc catcaccgca ccggcgtgga
     2041 gatggaggcg ctcaccggtg cggcggtggc ggctctgacg atctacgata tgtgcaaggc
     2101 gctttcgcac gatatcgaga tcggcagcgt acgcttgctc gcgaaagcgg gtggccgccg
     2161 ccgcttcgcg cgcagcgctg cggagcggcg cccgtgaact cgcagccagg gaacggccgc
     2221 accggcgccg cgccgctgct cgggctcgtg ctcgccggcg gtcgcagcac ccgcatgcgc
     2281 accgacaagg ccgcgttgct gtacgccggc cgcagccagc tcgagcgcgc catcgagctc
     2341 attgcgcccc atgtggaacg tagcttcgtc tcggtacgcg ccgatcagag tgaggacgcg
     2401 ctgcgcgcgc gcttcgcgca gatccgcgac gtgcacgaca atctcggtcc ggtcgcgggg
     2461 ctgctcgccg cgcaggcccg tcatcccgag gctgcgtggc tcgtgctcgc ctgcgatctg
     2521 ccgctgctcg atgcggcaac gctcgctaac ctggtgcgct gccgcgcgcc acagcgagcc
     2581 gcgacggcat atcgctcgag ccacgatggc ttacccgagc cgctgtgcgc gatctgggag
     2641 cctcgcagcg ctgcgccgct ggcggcctat gtggcacgcg gccggcagtg cccacgtcgt
     2701 ttcctgctcg atgcggacgt gcgcctgctc gaggagccca accccgaggc gctcgacaac
     2761 gtcaacacgc ccgaggagta taggagcgtc atgagcgcgc tcctgccgga accgacttcc
     2821 gggcccaaac aaatcaccgt gcagtactac gcgttgttgc gcgagcaagc cggtcggcgc
     2881 gatgaggcgc tggtgaccag cgcgcgcacc gcggcggagc tctatgcgga gctcgcgcgg
     2941 cgctacccct tctcactcgc accggaagtg ctgcgcgttg cgatcaatgc cgagttccgc
     3001 gagtggactg cggcactcgc cgatggcgat gccgtagtgt tcataccacc cgttgccggc
     3061 ggctgagccc tgcacacgac catggccgcg ttccggttca tgcgtacacc gatcaccacc
     3121 gaggagctgc gcgtggcgct cgccgacccg gcttgcggcg gctatgccac cttcgaaggc
     3181 tgggtgcggg accgcaatga gggtcagcgc gtgcgcacac tcgagtacga ggccttcgag
     3241 gcgctcgggg tcggcgaggg tgagcgcatc atcgccgagg cgcgtgagcg cttcggcgta
     3301 gcgcacgcgg cctgcgtcca tcgcatcggc gtgctcgcgg tcggcgagct cgccgtctgg
     3361 gtgggggtga gcgccgcgca tcgcgacgag gctttccgcg cctgccgcta catcatcgat
     3421 gaggtcaagc accgtctgcc catctggaag aaggagcact acctgaacgg tgactccggc
     3481 tgggtgaact gcgagcgctg cgccacggcg ccgaaacacg cggacgccca ggcctgaccg
     3541 gtagcactgc gccgctgccg ccggagtgcg cagtcaaccc acgcttacac gatggttcac
     3601 cagatggtga actattggca cgagcaacct catctgcaca cggctctacg gtcgtacctg
     3661 ccccgttccg cgcacaaggt acttgtaact ggtgagctgc tccacgccga ctgggccgcg
     3721 cgcgtggaag cggtcggtgg aaatcccgat ctcggcgccc ataccgaact caccgccatc
     3781 ggcaaactgc gtcgaggcgt tgtgcaggac gatcgcgctg tcgacacgct gcatgaagcg
     3841 ttccgcggtt gcggcgttct cggtgacgat cgattcggtg tgcgcggatc cgtagcgggc
     3901 aatgtgcgcg atggccgcat cgaccccggc gactacccgc gcagcgatga tggcgtccag
     3961 gtactccgtg taccaatcgt cctcgctcgc cgggcgcacc cgcggatcga ccgcctgcac
     4021 ggccgggtcg ccgcgcacct cgcagccggc cgcgagcaga tcgcggatga cgggtgcgag
     4081 atgtgtcgcc gcgcaggctt catcgacgag cagcgtctcg gccgcgccgc agatgccggt
     4141 gcgacgcatc ttggcattga gcgcgatggc gcgtgccatg cgcacatcgg catcccggtc
     4201 gatataaacg tgacagttgc cctccaggtg cccgatgacc ggcacgcgcg cttcccgctg
     4261 cacgcgctcg acgaggctgc gcccaccacg cggcacgatg acatcgagat actctcccat
     4321 gcccgcgagc aggtagccga ccgcggcgcg atcgctggtc ggtaccagct ggatgcaacc
     4381 gagcggcaga tccgcggcag cgagaccgct cacgaggcac tcgtgcagcg cgtggctcga
     4441 gtactggctc tccgagccgc cacgcaggat gcaggcattg ccggatttca ggcacaacgc
     4501 accggcatct gcggtgacgt tggggcgact ctcgtagatg atgccaatca cgccaagggg
     4561 cacggtgatg cgctggatgc gcaagccatt cggccgttcc cactcggccg ccacgcgccc
     4621 gatgggatcg ggaagcgcca gcaccgcatc gatacctgct gcgatcgctg cgatgcgccg
     4681 ctcatcgaga cgcagccgct cgagccgcgg gccgctgagc cgacgcgcac gcgcggcgga
     4741 catatcgcgt tcgttggcgg caagcagggt agccgtccgc gcccgtatcg ccgccgcggc
     4801 cgcagcgagc gcggcgtcct tctgggtgct cgaggcgagc gccagcacct gcgcggccgc
     4861 gaccgcggtg cggccgaatc cctccatcaa ctcgccgata cctgccgcgt ccatcggttc
     4921 agcgggccgg ttgtggggtg gccgccaccg ctgcggcagc atactcctgg cgcagcagca
     4981 ccaggtcgtc ccggtggatc atctcgtcgc ggccgcggaa cccgaggatc tcggcaatct
     5041 ccgagctcct gcggcccatg atgcgcgcgg cgtcgctgtc ggagtaggca atgatgccgc
     5101 gcgcgacctc gacaccgtcg gcggcgagca cgctcaccgt gtcgccgcga tcgaatctgc
     5161 cgcgcgtgcc ggtgacaccc gcgggcagca ggctgcggcc ttcgagcaag gcccgcagcg
     5221 cacccgcgtc gatggtgatg gcgcccgcgg gacgcagcgt gccggcgatc cactgcttgc
     5281 gcgccgcggc gggagtcgcg gtgggaatga accaagtgca ttccgcgccc tcctctatgc
     5341 ggcgcagcgg atggcgcggt gcgcccgccg cgatgcacat gtgacagccg gcggcgagcg
     5401 cgatgcgggc cgcggcgatc tttgcggcca tgccgcccga gccgagctgc gaggcggtgc
     5461 gccccgccat ggcttcgatc tcgggcgtga tctgccgcac ctcgtggatg aagcgcgcgc
     5521 tggggtcgcg ggtgggatcc gcactgaaca gtccggcgac atccgataac agcaccaggc
     5581 agtcggcgcc cgccatctgc gccacgcggg ccgcgagccg atcgttgtcg ccgtagcgga
     5641 tttcggcggt cgccaccgtg tcattctcgt tgatgaccgg gagcgcgccg agctccagca
     5701 gtgactcgag cgtggcgcgc gcgttgagat aacggcggcg gcgctcgctg tcctcgaggg
     5761 tgagcagcac ttgcgccacg gtcacgtcat tgctctcgag cagctccttg taggcgtgcg
     5821 caaggcggat ctgtccgacc gcagcggctg cctggctctc ctccaggcgc agcgtcccat
     5881 gcttgagacc gagacgccgc cggccgagag cgatcgcccc tgatgacacc aggatgacac
     5941 gctgtccgcg cttgcgcaga cgcaacagat cctcgatcag ggtctcgagc caggtgcgat
     6001 tgacgcgagc gctctcggcg tccaccagca gtgcagagcc caccttcacg acgatgcgcc
     6061 gtgcctgcgc gagtggcacc gccgaaacaa tgagttcgcc ggccatgaag gaaaactata
     6121 cgcatccggc ccgcgttgcc caacccgacg ccacccccta cacttgtcgg cggcgaaaat
     6181 gaacacaggg tggttttcgc ggtgagcaga gattacgagc cggtgcccgg tcagtggtac
     6241 gaaaacctcg aagaagaaga atccttccgc gtactgtcgg tggatgagga ttccgagcgt
     6301 ctggagatcg agtatctcga cggcgacatc gaggaaatcg acctcgagac gtggcacgag
     6361 atggacctcg agcgcatcga ggagcccgag ggctggtccg agtccgacga tgaagacgcc
     6421 gaggacgacg acgatgactg ggacgaggac gaagaagacg aagacgacga cgattgggac
     6481 gaggacgagg acgaggccga cgatcagcgg gatgagtatt gaccgcggcc ggcgtcgata
     6541 gctggtatca cgagaaggag tccgcctggc tctaccggca ggtggcggct gccgaggcgg
     6601 acccgctcaa aagcgcgctg tttctgaagc tggcggcggc tgccgaggag caggcggcca
     6661 agtgg
//
LOCUS       DATDMP010000159         2391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_580371, whole genome shotgun sequence.
ACCESSION   DATDMP010000159 DATDMP010000000
VERSION     DATDMP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580371"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..679
                     /gene="pgeF"
                     /locus_tag="VI195_02650"
     CDS             26..679
                     /gene="pgeF"
                     /locus_tag="VI195_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY6823315.1"
                     /translation="MRDLIRPQWPARVGALVTTAATGDIASEEGRARLRAELPGEPRW
                     LQQVHGARVVDADRLAGPEKADASVARNPGTVCVVKIADCMPVLFADEDAAVVAAAHA
                     GWRGLAGGVLEATIEAMRAPPERLYAWLGPAIGPRVYEVGEDVRAAVGEPRDAFVPTR
                     PGHWLLDLYAVARDRLMKHGVQRIYGGGYCTFSERERFFSYRRDHDRRRMAAAVWLA"
     gene            693..1454
                     /locus_tag="VI195_02655"
     CDS             693..1454
                     /locus_tag="VI195_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY6823316.1"
                     /translation="MTLGWILAASIAGGTLSAALAAFALYLRASWMPMLISYAIGALL
                     GASFLEVIPNAFELGAPRQAAGAILAGIFGFFVLEKLLLWRHCHTENCEVHDQHMQVH
                     DHGRSGALIMVGDTVHNFVDGVLIAAAFMQSTELGIIAALAIIAHEIPQEVGDFLILL
                     HSGYTRGQALAVNLLSSTATIFGGVLGFYGLQFVREWEGALLGFVAASMIYVAVADLI
                     PGLHRRPQLRDTASQALLIALGIGSIAGVRMLLAH"
     gene            1601..>2391
                     /locus_tag="VI195_02660"
     CDS             1601..>2391
                     /locus_tag="VI195_02660"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823317.1"
                     /translation="MRLFSSPPPVGQNSALQAAYLEQQTKLWTALLAGRKESLVEEDD
                     RRFAAREWRENPYFNYLRQSYLLAAKYLEQMVESAPLDATQKERARFAARQWIDAMCP
                     ANFPATNPVALREALDTKGESLTRGLANLVNDAQRGRISQTDESAFELGKNVATTPGD
                     VVFENDLIQLIQYRARTEDVAKRPLVMIPPCINKYYILDLQPENSFVGYAVDEGHTVF
                     MVSWRNVGPEQGHYGWDDYLEQGVFTALRVAQEIAKSDKVNALGF"
BASE COUNT          387 a          775 c          819 g          410 t
ORIGIN      
        1 agcgcctggt cgacgagctg cgcgcatgcg agatctgatc cggccgcaat ggccggcgcg
       61 cgtcggcgcg ctggtgacga cggcggcgac gggcgacatc gcgagcgaag agggccgcgc
      121 gcgcctgcgc gccgagctgc ccggggagcc gcgctggctg caacaggtgc atggtgcgcg
      181 ggtggtggat gccgatcgcc ttgcggggcc agagaaggcc gatgcgtcgg ttgcgcgcaa
      241 tcccggcacg gtgtgcgtgg tgaagatcgc cgattgcatg ccggtgctgt tcgccgatga
      301 agacgccgcc gtggtcgcag cggcgcacgc cggctggcgc ggccttgcag gcggtgtgct
      361 cgaggcgacg atcgaggcaa tgcgcgcgcc gcccgagcgg ctgtacgcat ggcttggccc
      421 ggcgatcggg ccgcgcgtgt acgaagtggg cgaggacgtt cgcgcggcgg tcggcgagcc
      481 gcgcgacgcc ttcgtgccga cgcgtccggg acattggctg ctcgatctct atgcggtggc
      541 gcgcgaccgg ctgatgaagc acggcgtgca gcgcatctac ggtgggggtt actgcacgtt
      601 cagcgagcga gagcgctttt tttcgtatcg ccgcgatcac gatcggcgcc gcatggccgc
      661 ggctgtctgg ctcgcctaga atcccaaccc ccatgacgct cggctggatc ctcgccgcaa
      721 gcatcgccgg cggcactctc tccgcggcgc tcgctgcctt cgcgctgtac ctgcgcgcga
      781 gctggatgcc gatgctgatc tcctacgcga tcggcgcgct gcttggcgcc tcgttcctgg
      841 aggtgattcc gaatgcgttc gagctcggcg cgccgcgcca ggcggccggc gccattctcg
      901 ctggcatctt cggcttcttc gtgctggaga agttgctctt gtggcgccat tgccacaccg
      961 aaaactgcga agtgcacgac cagcacatgc aggtgcacga ccatggccgg agcggcgcgc
     1021 tgatcatggt tggcgatacc gtgcacaact tcgtcgacgg ggtgctgatc gccgccgcct
     1081 ttatgcaaag caccgagctc ggcatcatcg cggcgctcgc gatcattgcg cacgagattc
     1141 cgcaggaggt cggggatttc ctgatactcc tccattcagg ctacacgcgg ggccaggcgc
     1201 tcgccgtcaa cctgctctcc tccacggcga cgatcttcgg cggcgtgctt ggcttttatg
     1261 gcctgcagtt cgtgcgggaa tgggaaggag cgctgctcgg cttcgtcgcc gcgagcatga
     1321 tttacgtggc agtggccgac ctgatccccg ggttgcaccg gcggccgcaa ctgcgcgata
     1381 ccgcctccca ggccctcctt atcgccctcg gcatcggcag catcgcgggg gtccgcatgc
     1441 tgctcgcgca ctaaggtaaa aggaccacgc ttcagtaggt tattgggcaa ttgacgccgg
     1501 gggttgtgcg tcgcaaaatc ggttacggat gcaacctggc tctccagatg ccctgcttgc
     1561 ggccatcgcg cagtccggga atgcagtcac ccagggctgg atgcggctct tctcttcgcc
     1621 accgccggtc ggacagaaca gcgccttgca ggccgcgtat ctcgagcagc agaccaaact
     1681 gtggacggcg ctcctcgccg gccgcaagga atcgctggtc gaggaagacg atcgccggtt
     1741 cgccgcgcgc gaatggcgcg aaaacccgta cttcaactat ctgcgccagt cgtacctgct
     1801 cgcggcgaag tatctcgagc agatggtgga gagcgcgccg ctcgacgcga cgcagaagga
     1861 gcgcgcgcgc ttcgccgcgc ggcaatggat cgacgccatg tgcccggcaa acttcccggc
     1921 aaccaatccc gtggcgctgc gcgaggcgct cgacacgaaa ggcgagagcc tgacgcgcgg
     1981 cctcgccaac ctggtcaacg atgcgcagcg cgggcgcatc agccagaccg atgaaagcgc
     2041 cttcgagctc gggaagaacg ttgccacgac gccgggcgat gtcgtgttcg agaacgatct
     2101 gatccagctc atccagtacc gcgcgcgcac cgaggacgtg gcgaagcggc cgctggtgat
     2161 gatcccgccg tgcatcaaca agtactacat cctcgacctg cagccggaaa actccttcgt
     2221 cggctacgcg gtggacgagg gccacacggt attcatggtg tcctggcgca acgtcggccc
     2281 ggagcagggc cattacggct gggacgatta tctcgagcag ggcgtattca ccgcgttgcg
     2341 cgtggcgcag gaaatcgcca agagcgacaa ggtcaacgcg ctgggcttct g
//
LOCUS       DATDMP010000160         2763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1024342, whole genome shotgun sequence.
ACCESSION   DATDMP010000160 DATDMP010000000
VERSION     DATDMP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024342"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..776
                     /locus_tag="VI195_02665"
     CDS             141..776
                     /locus_tag="VI195_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6823318.1"
                     /translation="MAAVLKDRIRVVLADDHPVVRDGLAAIVNQQDDMEVVGEAGDGE
                     EAIKLYEQHRPDVMVLDLRMPRRDGVAVVQRVLEINPKACILIMTTYDGDEDIFQCLS
                     QGAKGYLLKDAPRQEILSAIRAVSEDRPYTSSTVAAKALQRMAKPSLTQRELDVLQLV
                     AEGRSNKDIARRLSITEGTAKTHVKAILTKLDAISRTEAVAVAHKRGLIHL"
     gene            960..2762
                     /locus_tag="VI195_02670"
     CDS             960..2762
                     /locus_tag="VI195_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6823319.1"
                     /translation="MNAHIALRQPDAESAPCAKTLTRINESLQARERLLTASARASRL
                     LLEAPDVRAAIPAVLGLIGEAASVDRVNLMQTRTGPQGERLLVVVNEWTAEHGARAQP
                     AATVCRCDEARYASVFAELRAGRSVCFSKGESSAGQVSAIEGVGTQTKAIVPIFVAGE
                     FDGVVGFDNTRGQRAIDAAELAALETAAGVIGAALHRERLVDDVRRERERAAEQRVAE
                     LAKANAVIRGNLERLAGETDLYSFMGHMLLEATRQFGASSGDVVVRDDALQEWRVVAH
                     VHDDQLTAAPYASSVPLEGSAFTALHGDELRQVLYFDIERKADGSWPGLMEFHLANGE
                     VGTVIYPMMFGGRSVGFMALTFKRNAEEVTRSELLVALAQQATLAVQLTRLAYSAKEA
                     AVLMERTRIGQEIHDGLAQAFTGILLQLGAVEEFPSCRKRGSELALTLSRIRELARDG
                     LAEARRSVMALRLDQTRRAGLELALRQLADRSTVPGGVSCTFEGAGIATGLKPEHEHE
                     LLRIAQEAVSNAVRHARPQTVRITMTDQLEQWELAVADDGVGMEQSPELSARQGFGLA
                     SMRQRASAIGGEWQIASEPGRGTRVSVRMMKRAS"
BASE COUNT          464 a          870 c          995 g          434 t
ORIGIN      
        1 ccgagctctc ggcgcggcag gggtttggcc tcgccagcat gcgccagcgc gccagtgcga
       61 tcggcgggga gtggcagatc gccagtgagc cgggccgcgg cacgcgtgtc agcgtgcgca
      121 tgatgaaacg agcctcctga gtggcagccg tgctgaagga ccgcattcgt gtcgtgctcg
      181 ccgatgacca cccggtggtg cgcgatggac tcgctgcgat cgtcaaccag caggacgaca
      241 tggaagtggt cggcgaagcc ggcgatggcg aggaagcgat caaactctac gagcagcatc
      301 ggccggatgt gatggtgctc gatctgcgca tgccgcggcg cgatggcgtg gcagtcgtgc
      361 aacgggtgct cgagatcaac ccgaaagctt gcatcctcat catgaccacg tacgatggcg
      421 atgaggacat ctttcagtgc ctgtcgcagg gcgcgaaggg ctacctgctg aaagatgcgc
      481 cgcgccagga gattctctcg gcgatccgcg cggtgagcga ggatcgcccc tacacctctt
      541 ctacggtggc cgccaaggcg ctgcagcgca tggccaagcc gagtctcacg caacgcgagc
      601 tcgacgtgct gcagctggtg gccgaagggc gcagcaacaa ggacattgcg cggcggctct
      661 cgatcaccga gggcacggcc aagacgcacg tcaaggcaat cctcaccaag ctcgatgcca
      721 tcagccgcac cgaagcggtc gccgtcgcgc acaagcgcgg tctgatccat ctataaggct
      781 ctcgagatcg catcggcgag tcggctcgcc atgggtatcc gccccggtgc gacgaagggt
      841 gctgcgctgc accagcacga gcgcctccct taactccttg attcgcaggc cggtctcagc
      901 ctgctcgctg gggcgaggta gacttgcggc cccgttgcgg accaggatgg ttacgcccaa
      961 tgaatgccca catcgcgcta cgacaaccgg atgccgaatc ggcgccctgt gcaaagacgc
     1021 tcacacggat caacgagtcg cttcaggcgc gggagcggct gctcaccgcc tcggcgcgcg
     1081 cgagccgcct gctgctggaa gcacccgacg tgcgcgcggc gattccggct gtgctcggac
     1141 tgatcggcga ggccgcgagc gtcgaccgcg tcaatctcat gcagacccgc accggtcccc
     1201 agggcgagcg gttgctcgtc gtggtgaatg agtggacagc ggagcacggg gcacgcgccc
     1261 agccggcagc caccgtgtgc cgctgcgatg aagcgcgtta cgccagtgtg ttcgccgagc
     1321 tgcgcgccgg gcgcagcgtc tgtttcagca aaggtgagtc ctcagcgggg caggtctccg
     1381 ccatcgaggg cgtcggcacg cagaccaagg cgatcgtgcc gatctttgtc gcgggtgagt
     1441 tcgacggggt ggtgggattc gacaacaccc gggggcagcg ggcgatagac gccgcggaac
     1501 ttgcggcact ggagaccgcc gccggtgtga tcggcgcggc gctgcaccgc gagcggctcg
     1561 tcgatgatgt acggcgcgag cgtgagcggg ccgccgagca acgcgtggcg gagctcgcca
     1621 aggcgaacgc ggtgatccgt ggcaacctcg agcggcttgc cggggagacc gacctataca
     1681 gcttcatggg ccacatgctg ctcgaggcca cgcggcagtt cggggcgagc tcgggcgatg
     1741 tagtggtgcg ggacgatgcg ctccaggagt ggcgcgtggt cgcacacgtg cacgatgacc
     1801 agctgacggc agcgccctac gcgagcagcg tgcccctgga aggctcggcg ttcaccgctc
     1861 tgcacgggga tgagctccgc caggtgcttt atttcgacat cgaacgcaag gcggacggaa
     1921 gctggcccgg actgatggaa tttcacctcg ccaacgggga ggtgggcacc gtgatctacc
     1981 cgatgatgtt tggcggccgt agcgtcggtt tcatggcctt gacgttcaag cgcaatgccg
     2041 aggaagtgac gcgcagcgag ctgttggtgg cgctcgcgca gcaggcgacg cttgccgtgc
     2101 agctcacccg tcttgcctac tcggccaagg aagccgcggt gctcatggag cgcacgcgca
     2161 tcggccagga aatccacgat ggtctggcgc aggccttcac cggcatcctg ctgcagctgg
     2221 gcgcggtgga ggagtttccc tcctgccgca agcgtggctc ggagctggcg ctcacgctct
     2281 cgcgcatccg tgagctggcg cgcgatgggc tcgccgaggc gcgccgctcg gtgatggcgc
     2341 tgcgtcttga ccagacgcgc cgcgcggggt tggagctggc gctgcgtcag ctcgccgatc
     2401 gctcgacggt gcccggcgga gtgagctgta cgttcgaggg tgcgggcatc gccaccgggc
     2461 tcaagcccga gcacgagcac gagctgctgc gcattgcgca ggaggcggtg agcaatgcgg
     2521 tgcggcatgc gcggccgcag accgtgcgca ttaccatgac cgatcagctc gagcagtggg
     2581 agctggccgt tgccgatgat ggtgtcggca tggagcagag ccccgagctc tcggcgcggc
     2641 aggggtttgg cctcgccagc atgcgccagc gcgccagtgc gatcggcggg gagtggcaga
     2701 tcgccagtga gccgggccgc ggcacgcgtg tcagcgtgcg catgatgaaa cgagcctcct
     2761 gag
//
LOCUS       DATDMP010000161         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_469097, whole genome shotgun sequence.
ACCESSION   DATDMP010000161 DATDMP010000000
VERSION     DATDMP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469097"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VI195_02675"
     CDS             complement(<1..1423)
                     /locus_tag="VI195_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY6823320.1"
                     /translation="MRPRRRDAQKSAPGGPAERSLRYRHLRNPFAPVRVFSDDHIESM
                     HVAALGIIERQGMRVLSARGRAVLGAAGASVDEASQMVRLDPAMVSGALATVPAEVTL
                     IGRDPARSCRVGGQHVVFAPVAGPPSVSDLERGKRTGSIADFRDFVRLSQAFDVLHVL
                     AQTTEPQDVPVNERHIETALAQLTLADKAPNFYSRGDAQLADCFAMLRIAHGIDEATF
                     RAAPRCFSICNTNSPLQLDVPMTDGIMEFALNGQLMIVTPFTLAGAMAPVTIPGALTL
                     AHAEALFGITLSQTVRPGAPVMYGSFTSNVDMKSGSPAFGTPEYTKAAFGAGQLARRI
                     GMPWRCSSATASNAPDAQAAYEAQMSLWGALLGGCNFMLHAAGWLEGGLTASFEKFIL
                     DVEMLQMFAELFQPVAATAEEIGIDAVAEVGPGGHFFAAAHTMQRYRDAFYTPLISDW
                     RNYGAWVEAGAKSATERATSVWRQ"
     gene            1858..>2568
                     /locus_tag="VI195_02680"
     CDS             1858..>2568
                     /locus_tag="VI195_02680"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY6823321.1"
                     /translation="MPSRVPEGQERGWIIPIGGAENKENDRRILERFVRVSGGDQADI
                     VVIPTASRMHETGPRYEQIFTALGAARVTVMDFDTRRDCREAGRLKRIEEASGIFFTG
                     GNQLRLTTLLGGTPVAKLIRVRNARGVTVGGTSAGASILCEHMIAGGDEGSSIVAGSV
                     RLAPGLGLTNRFIIDQHFRERDRLGRLLTAVAYNPFAVGIGLDEDTAAFIGPDEVLEV
                     EGSGSVTLVDPSEVSFSSI"
BASE COUNT          435 a          824 c          877 g          432 t
ORIGIN      
        1 tctggcgcca cacgctcgtg gcgcgctcgg tggcgctctt ggcgcccgcc tccacccagg
       61 cgccgtagtt gcgccagtcg gagatcagcg gagtgtagaa ggcatcgcgg tagcgctgca
      121 tggtgtgggc ggccgcgaag aagtgacccc ccggcccgac ttcagctacc gcatcgatgc
      181 cgatctcctc tgcggtagcc gctaccggct ggaagagctc cgcaaacatc tgcagcatct
      241 cgacatcgag aatgaatttc tcgaacgagg ccgtgagccc gccttccagc catccggcag
      301 cgtgcaacat gaagttgcac ccgccgagca gcgcgcccca caagctcatc tgcgcttcgt
      361 acgctgcctg ggcgtcgggt gcattcgacg ccgtcgcgct cgagcagcgc catggcatac
      421 cgatacgccg cgcgagctgt ccggcaccga aggccgcctt ggtgtactca ggcgtgccga
      481 aggccggcga gccggatttc atgtcgacgt tagaggtgaa gctgccgtac atgaccgggg
      541 cgccgggacg gacggtctgc gacagggtga tgccgaagag cgcctcggcg tgcgcgagtg
      601 tcagcgcgcc ggggatggtc accggcgcca tggcgccggc gagcgtgaag ggcgtgacga
      661 tcatgagctg gccgttcagc gcgaactcca tgatgccgtc ggtcatcggc acatcgagct
      721 gcaacggcga gttggtgttg cagatgctga aacagcgcgg tgcggcccgg aaggtggcct
      781 catcgatgcc gtgggcgatg cgtagcatcg caaagcagtc ggcgagctga gcatcgccgc
      841 ggctgtagaa gttgggcgcc ttgtcagcca gggtgagttg cgcgagcgcg gtctcgatat
      901 gtcgctcgtt caccggcaca tcctgcggct cggtggtctg cgcgagaacg tgcaacacgt
      961 cgaacgcctg cgacagccgc acgaaatcgc gaaaatccgc gatcgagccg gtgcgtttgc
     1021 cgcgctccag gtcgctgacg ctaggtggac ccgcgacggg cgcgaacacg acgtgctggc
     1081 cacccactcg acaggagcgg gccgggtcac gcccgatcag ggtcacttcg gccgggaccg
     1141 ttgccagcgc gccactcacc atcgccggat cgagccgtac catctgactc gcctcatcga
     1201 cactcgcgcc cgcggcgccg agcaccgcgc ggccgcgcgc ggagagcacg cgcatgccct
     1261 ggcgctcgat gatgccgagc gctgcgacgt gcatgctctc gatgtgatcg tccgaaaaga
     1321 ctcttaccgg cgcgaatgga ttgcgcaggt gacggtagcg gagcgagcgc tccgccggtc
     1381 cgcccggcgc gcttttctgg gcatctcgcc tgcgcggtct catgatggtt gttgcacccg
     1441 cagccggcgc ccggcgggcc gctgccgcta tggttcggga gcgcaataga gcccgagggg
     1501 gcaagtccgc gtcaagcggg gaaaaggcat agccgccggc aaaaatgcat agcgtcgcaa
     1561 tgttgcgccc accgtcaggc gttgggggtt gaagccggcg ggagtctcaa catgttgata
     1621 ccccggtata tcaacacatt tacattcggg cggtatgcct cctggtcctc cccggccgga
     1681 tccggtctca cgggcatggc gtcgcggcgt tgcgacgcgg cggcgcgaca cacgtgtagc
     1741 ggaccgctca ttccgccgta ggactcggcc gatccgctgc atgcaacgcg cgcccgttgc
     1801 gccggcgtgg taactttgac tcccccgcgc agcggatatt caaggagtca gcaacggatg
     1861 ccgagcagag ttcccgaggg tcaagagcgc ggctggatca ttcccatcgg tggcgcggag
     1921 aacaaggaga atgaccggcg tatcctcgag cgcttcgtgc gcgtctcggg aggtgaccaa
     1981 gccgacatcg tggtgattcc gactgccagc cgcatgcacg agaccgggcc acgctacgag
     2041 cagatattca ccgcactcgg tgcggcacgc gtcaccgtca tggacttcga tacccggcgg
     2101 gactgccgcg aggcagggag gttgaagcgc atcgaggaag cgagcggcat cttcttcacc
     2161 ggcggtaacc agctgcgtct cacgacgctg ctcggcggca ctccggttgc caagctcatc
     2221 cgcgtccgca atgcgcgcgg tgtaaccgtc ggcggtacca gcgccggcgc cagcattctc
     2281 tgcgagcaca tgatcgcggg cggcgatgag ggctcctcca tcgtcgccgg cagcgtgcgc
     2341 cttgcgccgg gcctggggct gacgaaccgt ttcatcatcg atcagcattt tcgtgagcgc
     2401 gatcgactgg ggagattgct caccgcggtg gcctacaatc ccttcgcggt gggtatcggg
     2461 ctcgatgagg acaccgcggc tttcatcggg ccggacgagg tgctggaggt cgagggcagc
     2521 ggcagcgtga ccctggtgga cccttcagag gtgagctttt cttcgatt
//
LOCUS       DATDMP010000162         5613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1136044, whole genome shotgun sequence.
ACCESSION   DATDMP010000162 DATDMP010000000
VERSION     DATDMP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136044"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1659)
                     /locus_tag="VI195_02685"
     CDS             complement(172..1659)
                     /locus_tag="VI195_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY6823322.1"
                     /translation="MSKQASSTLGGCSTRGSARAGALLALTFAAAGIAQAQTAAPAPA
                     KTEDSSLTWKGITLYGIVDIGVTYQTHGAPASDYFPNGTYSVIQKFHNGSTTAVTPSN
                     LAQSRIGLSGNEPIAGDWAGVFRLETFFNPQSGNLSDALKSLALNNGKALSAYTTGAD
                     SSVAGQYFAGAAYAGFSSPTFGTITFGRHVTPLADGIAKYDPMGASQSFSLIGESGTT
                     AGAGDTENRRLDQSLKYTFKYNWLHAGALYQFSGASGSTNTAVQVTLGAEYAGLSFDA
                     YYGKKYNAVAASTLSAGQVASLQSNCTASITPPAAPSAQCYSVSNSLAGTISDNTDWS
                     VMGLYNFGVVKLYAGYEHVSFANPKNPSEPGQPIMGGYVLAIVNNTAFPNDKILQIFW
                     AGAKWTVTPELDLTVAYYGYKQNAYGTGADAGCSSVAHGVCSGTENVGSFVADYRLSK
                     RFDVYIGTIWSEVKDGLANGFLLTSDNTTSTAATITTTAGLRFKF"
     gene            complement(1699..3354)
                     /locus_tag="VI195_02690"
     CDS             complement(1699..3354)
                     /locus_tag="VI195_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823323.1"
                     /translation="MATTNLPMYESGIDRAHRRVIFASSLGTVFEWYDFYLYGSLAAI
                     ISKQFFSGVNETTGFIFALLAFAAGFAVRPFGALVFGRLGDMVGRKYTFLITIVIMGL
                     STALVGLLPSYAQVGVLAPVILIALRLLQGLALGGEYGGAATYVAEHAPHGKRGLYTS
                     WIQTTATLGLFLSLIVILLTRSFLGDQFDTWGWRIPFLISVVLLGVSVYIRLQLAESP
                     VFLEMKGAGTQSRAPLTESFARWGNLRVVVLALLGATAGQAVVWYGGQFYALFFLTQV
                     LKVAAQPANLLIAAALVIATPFFIVFGRLSDRIGRKKIVLLGCLLAAITYVPIFKGIT
                     HFANPAIESASVSAPVVVVADPRACTFQFDPVGKKKYVQSCDIAKAALAKAGIPYRNQ
                     AAPVGSVAMVLIGNEAVSSLEGERMSAGEFKTASGDFAKALGAKLKAAGYPANADPAQ
                     INYPMVLLLLVILVIYVTMVYGPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPP
                     ISFALVALTGDMYYGLRYPIIVALMTVVIGALFLPETKDRSIS"
     gene            complement(3585..5507)
                     /locus_tag="VI195_02695"
     CDS             complement(3585..5507)
                     /locus_tag="VI195_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6823324.1"
                     /translation="MATEADLLIHRLLMIDDNPRIHEDYRKILAGRDPGKVSSAEAAL
                     FGEQTQQQGPAFDVDSAMQGRDGVERARRALDEGRPYSVAFVDMRMPPGWDGLETIEQ
                     LWRIDPEVQVVVCSAYTDYDWLELLARLGHSDKLIVIKKPFEPIEILQSASALSRKWQ
                     NARALRHHIESLERAVTDRTRGLEAANRQLRHLASHDALTGLPNRLLLDDRVTQAVAQ
                     ANRQGHEFALLVVDLDRFKLINDSLGHRAGDELLREVAQRLRSAVRAVDTTARLGGDE
                     FVILVDGPVTRLEAVEIGQRAIDVMRPAMRMLGIDVHISPSIGIAFYPGDGATVDALL
                     ARADAAMYSAKERGRNNIQCYAEGMTTATQDRVRLESELHEALRAGQFELHYQPKVDT
                     SSGRINSAEALIRWRHPQRGLIPPKDFIPVAEECGLLDAIGKWVLFEACRQAKAWQEG
                     GLRPMRVAVNLAPSQFRLANLVEQIRTALEVAQLDPQYLEVELTESAVMSDAEESIVI
                     LESLSRMGVLVSVDDFGTGYSSMSYLRRFPIDKLKIDRCFVTDMTGRPEDASIVRAII
                     SLAHSLHLKVVAEGVETPEQLALLAELGCDQYQGFYFSPALLPAPFAELVKGSAEKRP
                     LTEDEAAQTHSKLARL"
BASE COUNT         1011 a         1713 c         1892 g          997 t
ORIGIN      
        1 tatggcggcc cggatctgaa tgcggcagcg gccgctgccg gcgcacaggc cgtgctcatg
       61 aagccgctcg ctgccgcaga ccttgcccgg tgcctggcgc aagtgctcgc cggcgcgcgc
      121 gccggatttg ccgcctgagg catgccgcac cgctcgtccg ggcggtgttc gtcagaattt
      181 gaagcgcagt cccgccgtcg tggtgatggt ggcagcggtg ctggtggtgt tgtccgaggt
      241 cagcaggaag ccgttcgcca gaccatcctt cacctcgctc cagatggtgc cgatatagac
      301 gtcgaagcgc ttgctgagac ggtagtcggc tacgaacgag ccgacatttt cggtgccgct
      361 gcagacgccg tgtgcgacgc tcgaacagcc ggcgtcggca ccggtgccat aggcgttctg
      421 tttgtagccg taataggcaa ccgtgaggtc cagctccggt gtgaccgtcc acttcgcccc
      481 agcccagaag atctgcagga tcttgtcgtt tgggaaagcc gtgttgttga cgatagcgag
      541 cacgtagccg cccatgatcg gctgccccgg ttcggagggg ttcttggggt tggcaaagct
      601 gacgtgctca taacccgcat agagcttcac cacgccgaag ttatagagcc ccatcaccga
      661 ccagtcggtg ttgtcggaaa tggtcccggc aagcgagttg ctcaccgaat agcactgcgc
      721 ggaaggggct gctggcggag tgatcgaagc ggtgcagttg ctctgcagac tcgccacctg
      781 tccggcactt agcgtagagg cggcgacggc attgtacttc ttgccgtagt aggcatcgaa
      841 ggacagcccc gcgtattccg cgccaagcgt cacctgcacg gcggtattgg ttgagccgct
      901 ggcaccggag aactgataca gcgctccggc atgcagccag ttgtacttga atgtgtactt
      961 cagcgactgg tcgagccggc gattctcggt gtcacccgcg cccgctgtgg tcccggactc
     1021 gccgatcagc gagaacgact ggcttgcccc catcgggtcg tacttggcaa tgccgtcggc
     1081 gagcggcgtg acatgccgcc cgaaggtgat cgtgccgaac gtgggggagc tgaagccggc
     1141 ataggcggct ccggcgaaat actgccccgc gacgctggag tcggccccgg tggtgtaggc
     1201 gctgagcgcc ttgccgttgt tgagcgccag cgacttcagg gcgtcgctga gattgcccga
     1261 ctgcgggttg aagaaggtct cgaggcgaaa taccccggcc cagtccccgg cgatgggctc
     1321 attgcccgag agaccgatgc gcgactgggc caggttgctc ggggtaacgg cggtggtcga
     1381 gccgttgtgg aacttctgga tgaccgagta cgtgccatta ggaaagtaat cgctcgccgg
     1441 agccccgtgc gtctggtagg tcacaccgat gtcgacgatg ccgtagagcg tgatgccctt
     1501 ccaggtgagg ctgctgtcct ccgttttggc aggcgcgggt gccgcagtct gtgcctgcgc
     1561 gattccggct gccgcgaatg tcagcgctaa cagcgctccg gcacgcgcgc tgccgcgcgt
     1621 actgcagcca cccaaggtcg aggacgcttg cttactcact ggaatcctcc aaatcgagtc
     1681 gtggttgtct ggaagaagtc agctgataga ccggtctttg gtttccggca ggaacagtgc
     1741 gcctatgacc accgtcatca gcgccacaat gatcgggtag cgcagtccgt agtacatgtc
     1801 gcccgtcaag gcgacgagcg cgaacgagat cggcggcagg aatcctccga accatccgtt
     1861 gccgatgtga taggggagcg acatcgaggt gtagcggatg cgcgccggga agagctccac
     1921 gagccatgcg gcgatcggcc cgtagaccat ggtcacgtag atcaccagga tcacgagcag
     1981 cagcagcacc atcgggtagt tgatctgcgc cggatcggcg ttagccggat agccggccgc
     2041 cttgagcttc gcgccaagcg ctttggcgaa atctccgctg gcggtcttga attcgcccgc
     2101 cgacattcgc tcgccttcga gcgaagacac cgcctcattg ccaatgagca ccatcgcaac
     2161 gctaccgacg ggggccgcct ggttacggta cgggatgccc gccttggcga gtgccgcctt
     2221 ggcgatgtca caggactgca cgtacttctt cttgccgacc gggtcgaact ggaacgtgca
     2281 ggccctcggg tccgccacca cgacgaccgg tgcgctaacc gaggcgctct cgatggccgg
     2341 attggcaaag tgagtgatgc ccttgaagat cggcacataa gtgatcgccg cgagcaggca
     2401 gccgagcagc acgatcttct tgcgtccgat gcggtccgag agccggccga agacgatgaa
     2461 gaacggtgtg gcgatcacga gtgccgccgc gatcaagaga ttggccggct gtgcggcgac
     2521 tttcagcacc tgcgtgagga agaagagggc atagaactgc ccgccgtacc agacgaccgc
     2581 ctggccggcg gtcgccccga ggagcgccag cacgacgacg cgcaggttgc cccagcgcgc
     2641 gaaagactcc gtgagaggcg cgcgcgattg ggtgcccgcg cctttcatct cgaggaacac
     2701 gggggattcg gccagctgca gtcggatgta gaccgagacc ccgagcagca cgacggaaat
     2761 gaggaaggga atccgccagc cccaggtgtc gaactgatcc ccgaggaagg agcgggtgag
     2821 caggatcacg atcaggctga ggaacaggcc gagggtcgcc gtcgtctgga tccagctggt
     2881 atacaagccc cgcttgccgt gcggcgcgtg ctcggcgaca taggtcgcgg caccgccgta
     2941 ctcgcccccg agcgcgaggc cctgcagcag gcgcaaggca atgagtatca ccggggcgag
     3001 gacacccacc tgcgcgtaac tcggcagaag tccgaccagt gcggtcgaga ggcccatgat
     3061 cacgatggtg atgaggaagg tgtatttgcg accgaccatg tcgccgagcc gcccgaatac
     3121 cagcgcgccg aacggacgca cggcaaagcc ggccgcgaag gcgagcaggg cgaagatgaa
     3181 tccggtggtc tcgttaacac cggagaagaa ctgcttcgag atgatcgcgg cgagtgatcc
     3241 gtagagatag aagtcgtacc actcgaagac cgttcccagg cttgacgcga aaatgacacg
     3301 gcggtgggcg cggtcgatac ctgattcgta catcggcaga ttggtggtag ccacgggata
     3361 tgctcctgtg tcggttgatt ctttatggtt ttttaccgct ggaccgaacc gtctgccaat
     3421 tccaacgaac cgctgagctg ggaacccaag ccgtggatgc gaatgtgaag cttggaggag
     3481 ttggatgtca gttgtatgac aggcccggca ccggcgagaa agcgagccga ggaaaggcgg
     3541 taaggcgcag gtcgtgcctg cgcccggaac tctgcgtgtc ctcttcagag acgggcgagc
     3601 ttgctatggg tctgtgccgc ttcgtcttcg gtgagtgggc gcttctcggc ggagcctttc
     3661 accagttccg caaatggcgc cgggagcagg gcggggctga aataaaaacc ctggtactgg
     3721 tcgcaaccga gctcggcaag gagggcgagc tgctcgggcg tctccacccc ttcggcgacc
     3781 accttcagat gcaggctgtg ggcgagtgag atgatcgcgc gaacgatcga ggcatcctcg
     3841 ggccgccctg tcatgtcggt gacgaagcag cgatcgatct tcagcttgtc gatcgggaag
     3901 cgccgtaggt agctcatgct ggagtagccg gtcccgaagt catccaccga taccagcact
     3961 cccatgcggc tgagcgactc gaggatgacg atcgactcct cggcatcgct catgaccgcg
     4021 ctctcggtga gctccacttc cagatactgg gggtcgagct gcgccacctc gagtgccgtg
     4081 cggatctgct cgacgagatt ggccaggcgg aactgcgaag gtgcgagatt cactgcgacg
     4141 cgcatcgggc gcagcccgcc ttcctgccac gccttcgcct gccggcaggc ctcgaacaac
     4201 acccacttgc cgatggcatc gagcagtccg cattcctcgg cgactgggat gaaatccttc
     4261 ggcggaatga gcccgcgctg cggatggcgc cagcggatca gtgcctcggc gctgttgatg
     4321 cggccgctcg aggtgtcgac cttgggctgg tagtgcagct cgaactgccc ggcgcgcagc
     4381 gcctcatgca gctcgctctc gaggcggacg cggtcctggg tggccgtcgt catgccttcg
     4441 gcgtagcact gtatgttgtt gcggccgcgt tccttggcgc tgtacatcgc ggcatcggca
     4501 cgcgcaagca aggcatcgac cgtggcacca tcgccggggt agaaggcgat gccgatgctt
     4561 ggggagatat gcacatcgat gccgagcatg cgcatcgctg ggcgcatcac atcgatggcg
     4621 cgctggccga tctcgaccgc ctcgaggcgc gtcaccggtc catcgaccag gatgacgaac
     4681 tcatcgccgc cgagccgtgc ggtggtatcg accgcgcgca cggcgctcct caggcgctgc
     4741 gccacctcac gcagcagctc gtcaccggcg cggtgcccga gggagtcgtt gatgagcttg
     4801 aagcggtcga gatcgaccac gagcagggcg aactcatggc cctggcggtt ggcctgggca
     4861 accgcctgcg tgacgcgatc atcgagcagc agccgattcg gcagccccgt cagggcatcg
     4921 tgcgaggcaa ggtgacgcag ttgacggttg gcggcctcga gccctcgagt acgatcggtg
     4981 accgcgcgct cgagactttc aatgtggtgc ctgagggcgc gggcgttctg ccacttacgg
     5041 ctgagcgcgc tcgctgactg caggatctcg atcggctcga acggcttctt gatgacgatg
     5101 agcttgtccg agtgcccgag gcgtgcgagc agctcgagcc agtcgtagtc ggtataggcg
     5161 gagcacacca cgacctgtac ttccgggtcg atcctccaga gctgctcgat ggtctccagt
     5221 ccgtcccagc ctggcggcat gcgcatgtcg acgaacgcga cggagtaggg tcggccttca
     5281 tcgagcgcac ggcgcgcgcg ctcgacgccg tcgcgtccct gcatggccga gtccacatcg
     5341 aaggccggcc cctgctgctg ggtctgctcg ccaaagagcg ccgcctcggc gctcgagact
     5401 ttcccggggt cgcgaccggc gagaatcttg cggtagtcct catggatgcg cggattgtcg
     5461 tcgatcatga gcagccggtg gatcagcagg tctgcctcag tggccatggc gagcctccgc
     5521 gtgggaaagc ggcagagtca gcgtgaaggt cgcgccctgg cccggaccgg cactggtcgc
     5581 atggagcgag cccccgattt ccttggcggc gag
//
LOCUS       DATDMP010000163         4061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_581708, whole genome shotgun sequence.
ACCESSION   DATDMP010000163 DATDMP010000000
VERSION     DATDMP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581708"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VI195_02700"
     CDS             complement(<1..1176)
                     /locus_tag="VI195_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY6823325.1"
                     /translation="MSPTPLGAGPGEGSLQAPTRHALDWRSAAFYDSAALDRELERVF
                     DICHGCRRCFSLCNAFPTLFDAVDATDAGEVAALDKKVFWQVVDHCYLCDMCFMTKCP
                     YVPPHPWNVDFPHLMLRAKALRARDGGLSWGERVLAATDTVGAFAGIPVVVEAVNALN
                     ASSAGRALLEKVLGVDRSAPLPSYHTRTARRRLAELGTVRAAHATADDAAVSARSATT
                     GRVALFTTCYGNRNEPELAEDLVAVLEHNGIEVALLGAERCCGMPRLELGDLEGVARL
                     KQRNIPQLLAAVQAGYDVLAPIPSCVLMFKQELPLMFPDDAGVRQVAARTFDPFEYLM
                     MRHAAGQLRTDFKVSLGKVSYHVPCHLRVQNLGLKTRDLLRLIPGTSIEPIERCSGHN
                     "
     gene            complement(1273..1686)
                     /locus_tag="VI195_02705"
     CDS             complement(1273..1686)
                     /locus_tag="VI195_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY6823326.1"
                     /translation="MKLKDSKTEKNLKDAFAGESQANRRYLYFAQKADVEGFNDVSAV
                     FRSTAEGETGHAHGHLEYLEAVGDPATGLPIGETRLNLKAAIAGETHEYTDMYPGMAK
                     IAREEGFGEIADWFETLAKAERSHANRFQKALDAI"
     gene            1828..2097
                     /locus_tag="VI195_02710"
     CDS             1828..2097
                     /locus_tag="VI195_02710"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY6823327.1"
                     /translation="MSGDNKPIDFEKALLELESLVERLERGDVPLDEALRTFERGVAL
                     TRHCQACLQAAQQKVEILLKRSGEPLVQPFEDSGEANTAGNADRE"
     gene            2151..3023
                     /locus_tag="VI195_02715"
     CDS             2151..3023
                     /locus_tag="VI195_02715"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY6823328.1"
                     /translation="MAECRTRIERVLERTLSLPDPGSARLREAMRYSVLGAGKRLRPT
                     LVYLTGEALGAPLAQLDAPAAAVELIHVYSLVHDDLPAMDDDDLRRGRPTCHRAYDEA
                     TAILVGDALQALAFAVLANEPLSAASAPLRLEMIRVLARATGTSGMAGGQAVDLAAVG
                     RTLTVTAIEDMHRRKTGALIQASVLLGALAAGVSGGAELAGLERFGAEIGLAFQIQDD
                     ILDVEGDAAVLGKSTGADAALSKPTYPSTVGLESARDRARELRDGAIAALAPLGSGGT
                     ALVQLANFIVNRAS"
     gene            3115..>4061
                     /locus_tag="VI195_02720"
     CDS             3115..>4061
                     /locus_tag="VI195_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY6823329.1"
                     /translation="MSEATDKYPLLERIATPADLRQLPSSKLPALARELREFLIQTVS
                     TRGGHFAAGLGTVELTIALHYIYDTPHDRLVWDVGHQAYPHKVLTGRRDRLHTIKQDG
                     GLAPFPTRTESEYDTFGVGHSSTSISAALGMAVAAARAGEQRQVVAIIGDGAMTAGMA
                     FEALNHAGSLPADLLVILNDNDMSISENVGALSNQFARALSGRVYAHLREGGKKVLRP
                     MPAVWELARRSEEHLKGMVLPGTLFEEMGFNYIGPMDGHDVKALVRTLRNIRKLRGPQ
                     FLHVVTRKGKGYAPAEADPIKWHGPGPFDPVSGTIFKEK"
BASE COUNT          691 a         1344 c         1353 g          673 t
ORIGIN      
        1 gttgtgaccg gagcagcgtt cgatgggctc gatgctggtg ccgggaatga gccgcagcag
       61 gtcgcgggtc ttcagcccga gattctgcac gcgcaggtgg cagggcacgt ggtagctcac
      121 cttgccgagc gacaccttga agtccgtgcg cagctgaccg gccgcatggc gcatcatcag
      181 atattcgaac ggatcgaacg tgcgcgcagc gacctgccgc acgccggcat cgtcgggaaa
      241 catgagcggc agctcctgct tgaacatgag gacgcacgag gggatgggcg cgagcacatc
      301 gtagcccgct tgcaccgccg ccagcagttg cgggatgttg cgctgcttga ggcgtgccac
      361 cccttcgaga tcgccgagct cgaggcgcgg catgccgcag caacgttctg cgccgagcag
      421 cgcgacctcg atcccgttgt gctcgagcac ggccaccaga tcctcagcga gctcaggctc
      481 gttgcgattg ccgtagcagg tcgtaaacag cgccaccctg ccggtcgtgg cgctacgtgc
      541 tgacacagcg gcatcgtcgg cggtcgcatg cgcagcgcgc acggtgccga gttccgcgag
      601 gcgccgccgc gcggtacgag tgtggtagct cgggagcggg gcgctgcgat cgacgccgag
      661 aaccttctcg agcaacgcgc gtccggcgct cgaggcgttg agcgcgttga ctgcctccac
      721 caccaccggg attccggcga aagcgcccac ggtatcggtc gcggccagca ctcgctcgcc
      781 ccaggagagc cctccgtccc gggcgcgcaa cgccttggcg cgcaacatga ggtgagggaa
      841 gtcgacgttc cacgggtgcg gcggcacgta tggacacttg gtcatgaagc acatgtcgca
      901 gaggtaacag tgatccacga cctgccagaa cacttttttg tcgagcgcgg ccacctcgcc
      961 cgcatcggtg gcgtccaccg cgtcgaacag ggtcggaaag gcattgcaca ggctgaagca
     1021 gcggcgacag ccgtgacaga tatcgaatac ccgctcgagc tcgcgatcga gcgcagcact
     1081 gtcatagaag gctgcgctgc gccagtcgag cgcatgccgg gtcggtgcct ggaggctccc
     1141 ttcgcctggg cccgcgccga gaggtgtcgg gctcatgggc tcatgaaccg cttaccgccg
     1201 gacgcagcgc acccgcagcc caggaggccg cggatgtgca ggacaccgca cgaacatcga
     1261 gcgcgcggcg tctcagatgg cgtccagagc cttctggaat cgattggcgt gggagcgctc
     1321 ggccttggcg agtgtttcga accagtcggc gatctcaccg aaaccctctt cgcgcgcgat
     1381 cttcgccatg ccggggtaca tgtcggtgta ttcgtgtgtt tcaccggcga tcgcagcctt
     1441 gagattcagg cgcgtctcgc cgatcggcag accggtggct ggatcaccca ccgcctccag
     1501 atattcgagg tgcccatggg cgtgaccggt ctcgccttcc gcggtcgagc gaaacaccgc
     1561 ggacacatcg ttgaagcctt ccacatcagc cttctgggcg aagtacaggt agcgccggtt
     1621 ggcctgcgac tccccggcga acgcatcctt cagattcttt tccgtcttgc tgtctttgag
     1681 cttcatgtcg ttcctcactt agactgggtc taatgcaggg gcggcactgt atcgccctgc
     1741 cgcagggtgc gcaaccctcg cgttgaccct tgccagcgcg cctgtgtaac gttgcccgcc
     1801 ctcgcgcaac tgcccgctcg gtgagacgtg tcaggcgaca acaaacccat tgattttgaa
     1861 aaggccctgc tggaactcga gagcctggtg gaacggctcg agcgcggcga cgtgccgctc
     1921 gatgaagcgt tgcgcacctt cgagcgcggg gtggcgctca cgcggcactg ccaggcgtgc
     1981 ctgcaggcag cgcagcagaa ggtcgagatc cttctgaagc gcagcggaga gcccctggtg
     2041 caaccattcg aggactccgg cgaggccaac accgccggga atgcggaccg ggagtgaccg
     2101 caacccccct taccggtgct gtcacggacg cgaaggtctt cgcaacgcgt gtggccgagt
     2161 gtcggacgcg catcgagcgg gtgctcgagc gcacgctatc gttgcctgac ccgggcagcg
     2221 cacggctgcg cgaggcgatg cgctacagcg tgctcggcgc cggcaagcga ctgcgaccca
     2281 cgttggttta cctcaccggg gaagcgctcg gtgcaccact tgcgcaactc gatgccccgg
     2341 cggcggccgt tgagctgatc catgtctaca gcctcgtgca cgatgacctg ccggcgatgg
     2401 acgacgatga cctgcggcgc gggcggccca cctgtcatcg cgcctacgat gaggcgacgg
     2461 cgatcctggt gggcgatgcg ctgcaggcgc tcgcgtttgc ggtgttggcg aacgagccgc
     2521 tctcggctgc gagcgcgcca ctgcggctcg agatgatccg agtgctggca cgcgctaccg
     2581 gtacgagcgg tatggccgga ggccaggcgg tggacctcgc cgcggtgggc cgcacgctca
     2641 cggtgacggc gatcgaggac atgcaccgcc gcaagactgg tgcgctcatt caggcgagtg
     2701 tgctgctcgg tgcgctcgcg gccggtgtca gcgggggggc ggagctcgcg ggcctcgagc
     2761 gttttggcgc cgagattggt cttgccttcc agatccagga cgacatcctc gatgtcgagg
     2821 gcgatgcggc cgtgctcggc aagtcgaccg gtgcggatgc tgcgctctcc aagcccacct
     2881 acccgagcac cgtcgggctc gagagcgctc gggatcgggc gcgggagctg cgcgatggcg
     2941 cgattgccgc gctcgctcca ctcggcagcg gcggcaccgc tctggtgcag ctcgctaatt
     3001 tcattgtcaa tcgagccagc tgagccgagg caggagcgca ctgccacgca gccacgcagc
     3061 cgcgcaccgc accctgatgc ggccctcctg cagctgcccg tgtaaactga cacgatgagc
     3121 gaggctaccg acaagtatcc acttctcgaa cggattgcta caccggccga cctgcgtcag
     3181 ctgccttcga gcaagttgcc cgcgctcgcc cgcgagctgc gcgagttcct gatccagacc
     3241 gtgagcaccc gtggcggcca tttcgcggcg ggcctcggta cggtcgaact caccatcgct
     3301 ctgcactaca tctacgacac gccccacgac cgactcgtct gggacgtcgg ccaccaggcc
     3361 tatccgcaca aggtgctcac cggccggcgc gaccgcctgc acaccatcaa gcaggatggc
     3421 ggactcgccc cctttccgac tcgcacggag agcgaatacg acaccttcgg tgtcgggcat
     3481 tcgagcacct ccatctccgc ggccctcggt atggcggtgg cggccgcgcg tgccggagag
     3541 cagcggcagg tggtagccat catcggtgac ggcgcgatga ccgcgggcat ggcgttcgaa
     3601 gcgctcaatc acgccggctc cctgcccgcg gacctgctgg tgatcctcaa cgacaacgac
     3661 atgtccatct ccgagaacgt cggggcgctc tccaaccagt tcgcgcgcgc tttgtcgggc
     3721 cgcgtctacg ctcatctgcg cgagggcggc aagaaagtgc tgcggccgat gccagcggtg
     3781 tgggagctgg cgcgccgctc ggaggagcac ctcaaaggaa tggtgctgcc cgggacgctg
     3841 ttcgaggaga tgggtttcaa ctacatcggt cccatggacg ggcacgacgt caaagcactg
     3901 gtacgcacac tgcgcaacat ccgcaaactg cgcgggcccc agttcctgca cgtcgtgacc
     3961 cgcaagggca agggttatgc gcctgccgaa gccgacccta taaaatggca cggacccgga
     4021 ccgttcgatc cggtgagcgg cacgatcttc aaggagaaga g
//
LOCUS       DATDMP010000164         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_137232, whole genome shotgun sequence.
ACCESSION   DATDMP010000164 DATDMP010000000
VERSION     DATDMP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137232"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..411)
                     /locus_tag="VI195_02725"
     CDS             complement(232..411)
                     /locus_tag="VI195_02725"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEY6823330.1"
                     /translation="MRPRSHSGAIVLILIGVVFLLVNLGVLPIRDLARLLSTWWPLIL
                     IAVGVWQLARTRKSE"
     gene            complement(429..1295)
                     /locus_tag="VI195_02730"
     CDS             complement(429..1295)
                     /locus_tag="VI195_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6823331.1"
                     /translation="MARPIRVFDGSFGRLQLIETAAGDHPQASAVPQIIVKQDGADLD
                     LRVDGDMLRLTRESLLFLSPGAASEAVFEAGAGSAQLLCFQASADWLRGSFPAVFDAD
                     SRAFGSARETITPRIRQLADTLVVEVLNDQFLSHERLEFMLQELMLSIVETYLARRQA
                     GARLWRGSPFADNRIRKALALLRAHPNKELNMNDLANQVGLSRSRFYDLFQTSTGRSP
                     RSYLDLLCVESAIAKLSGGSAKIAEVSAELGFSAQSNFTRFFQQQVGIPPSEYRRAAG
                     KPNTDPQDDPQG"
     gene            complement(1437..1823)
                     /locus_tag="VI195_02735"
                     /pseudo
     CDS             complement(1437..1823)
                     /locus_tag="VI195_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
     gene            1877..2218
                     /locus_tag="VI195_02740"
     CDS             1877..2218
                     /locus_tag="VI195_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY6823332.1"
                     /translation="MYVEERMYTLQVGTAAQYFKNYEEFGMKVQLRHLPHMVGYYVTE
                     VGELNKVIHLWAYDDLNQRDKCRAAMQADPDWAVYLAKNRPFMVAQEARVLKCAPFFV
                     ERLKKMLAAVK"
     gene            2224..>2987
                     /locus_tag="VI195_02745"
     CDS             2224..>2987
                     /locus_tag="VI195_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6823333.1"
                     /translation="MAAPHPQVVAILELVANSPLPPYHEVPPTVARRLYRDARGALTP
                     DPPAVESVRLLLAPGKAGPVPVRAYRPKGAGKDEVLPALVYFHGGGWVIGDLDTHDAV
                     CRTLANGARCAVFSVEYRKAPEAPFPAAVDDCFSALSFIFTNSNSLRINAGQIAVGGD
                     SAGGNLATVMALMARDAGAPAISFQLLIYPGVDMRMKHPSIKRNGEGYLLTEKVMAYF
                     RGHYVPREADWLDWRVSPLLAKSLKGLPPAFLLTAG"
BASE COUNT          513 a          991 c         1003 g          480 t
ORIGIN      
        1 gcgcgagtag acgtttccga aggtctggag attgaagagg ctcgcggcgt ggtcggccga
       61 gtcgaagacg aaggacgtgg tctggtagat cggcgcggcg cgcgcgccgg tggccggatc
      121 gggaatctgc ccggcgtgca ggcaaagcgt ctcgaagccg tacttgcggt cagccatcgc
      181 cggattttag cggcagtcgg cgacggcacg aggccgcgcc gcggctcgcc gctattcgct
      241 cttgcgcgtg cgcgcgagct gccagacgcc gacggcgatc aggatcagcg gccaccatgt
      301 cgacagcagg cgcgccagat cgcggatcgg cagcacgccc agattgacca gcaggaacac
      361 caccccgatc aggatcagga cgatggcgcc gctgtgcgag cgaggacgca tggccggaac
      421 ggtcccggtc aaccctgcgg atcgtcctgc ggatcggtgt ttggtttgcc ggccgcgcgc
      481 cggtactcgg aaggcgggat gccgacctgc tgctggaaga agcgcgtgaa gttggactgc
      541 gccgagaatc ccagctccgc ggacacttcc gcgatcttgg cgctgccgcc cgagagcttg
      601 gcgatcgccg actcgacgca cagcaggtcg aggtaggagc gcggcgagcg gccggtgctg
      661 gtctggaaca ggtcgtagaa gcgcgagcgc gacaggccga cctggttcgc caggtcgttc
      721 atgttgagct ccttgttcgg gtgcgcgcgc agcagcgcca gcgccttgcg gatgcggttg
      781 tcggcgaacg gcgagccgcg ccacaggcgc gcgcccgcct ggcggcgcgc gagataggtc
      841 tcgacgatcg acagcatgag ctcctgcagc atgaactcca gccgctcgtg cgacaggaac
      901 tggtcgttca gcacctcgac caccagcgtg tcggcgagct ggcggatgcg cggggtgatc
      961 gtctcgcgcg ccgagccgaa ggcgcggctg tccgcgtcga acaccgccgg gaagctgccg
     1021 cgcagccagt ccgcggaggc ctggaagcac agcagctgtg ccgagcccgc gcccgcctcg
     1081 aacaccgcct cgctggccgc gccgggactc aggaacagca ggctctctcg cgtcaggcgc
     1141 agcatgtcac cgtcgacgcg caggtcgaga tccgcgccgt cctgcttgac gatgatctgc
     1201 gggaccgcgc ttgcctgcgg gtgatcgccc gcggccgtct cgatcagctg caggcgaccg
     1261 aagctgccat cgaaaacgcg gattggacgg gccatgcgaa acagcttagc ggctggcaag
     1321 ccccttgcat aggggttctg gcgggagacc caatcgcgaa gctaattcgc taaaaaacgg
     1381 ggccttttcg ccgagatcac ccgggtgttg acccccaccc agttcaggcc gccgaacagg
     1441 cgcgcgtgct cgatcttcac gcagcggtcc atcaccgttt ccagacccgc ggcgcgcgcc
     1501 agggcggccg cctcttcgtt cttcacgccg agctgctgcc agagcacctt cgcgccgatg
     1561 gcgatcgcgt cccgggctac cggcagcacg tcggcggtct tgcggaacac gtccacgagg
     1621 tccactttct ccgggatatc gcgcaggttc gcgtagcatt tctggccgag cacctccgca
     1681 tattgcggat tcaccgggat cacgcggtag ccgtgttcca tcatgtactt cgccgcgaag
     1741 aagctgggcc ggtgccactg cgccgacagc cccaccacgg cgagcacgcg gcactctttc
     1801 aatatgcggc gcagggtatt gatgtcgtca tgcacccgac tactataatc gaacccacac
     1861 ccgggaggag gaagacatgt acgtcgaaga acgcatgtac acgctgcagg tcggcaccgc
     1921 ggcgcagtac ttcaagaact acgaggagtt cggcatgaag gtgcagctgc gccacctgcc
     1981 gcacatggtc ggctactacg tcaccgaggt gggcgagctg aacaaggtga tccacctctg
     2041 ggcttacgac gacctcaacc agcgcgacaa gtgccgggcg gcgatgcagg cagacccgga
     2101 ctgggcagtc tacctcgcca agaaccggcc gttcatggtc gcccaggaag cgcgcgtcct
     2161 gaaatgcgcg ccgttcttcg tggagcgcct gaaaaaaatg cttgcggcgg tgaagtaggg
     2221 ccgatggctg cgccgcatcc gcaagtcgta gcaatcctgg agctggtggc gaactcgccg
     2281 ctgccgccgt accacgaggt gccgcccacc gtagcgcggc gcttgtaccg cgatgcgcgc
     2341 ggagcgctca cgccggatcc gcccgcggtc gaatcggtgc gcctgctgct cgcgccaggc
     2401 aaagcaggtc ccgtcccggt gcgagcgtat cgacccaagg gcgcgggcaa ggacgaggtg
     2461 ctgcccgcgc tcgtctactt ccacggcggc ggctgggtga tcggcgacct ggatacgcac
     2521 gacgccgtgt gccgcacgct cgccaacggc gcacgctgcg cggtgttctc ggtcgagtac
     2581 cgcaaggcgc ccgaggcgcc ttttcccgcg gctgtggatg actgtttttc ggccctttcc
     2641 ttcattttca cgaattcaaa ttcgttgaga ataaatgcag gacagatcgc tgtaggcggc
     2701 gacagcgcgg gcggcaacct cgccaccgtg atggcgctca tggcgcgcga cgcgggcgcg
     2761 ccggcgattt ccttccagct gctcatctac cccggcgtcg acatgcgcat gaagcatccc
     2821 tccatcaagc gcaacggcga gggctacctg ctcaccgaga aagtcatggc ctacttccgc
     2881 ggccactacg tgccccgcga ggccgactgg ctggactggc gcgtctcgcc gctgctggcg
     2941 aagagcctga agggcttgcc gcccgcgttc ctgctcaccg cgggctt
//
LOCUS       DATDMP010000165         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1136673, whole genome shotgun sequence.
ACCESSION   DATDMP010000165 DATDMP010000000
VERSION     DATDMP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136673"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..889
                     /gene="gph"
                     /locus_tag="VI195_02750"
     CDS             131..889
                     /gene="gph"
                     /locus_tag="VI195_02750"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY6823334.1"
                     /translation="MHADSKTHTPRAGPRWGAVRAVLFDLDGTMLDTAADITEALNRA
                     LAEQGLPTLARNEVSELIGRGVPALIERVLERFGAAASTADATRLLERFHHHYQCLHQ
                     RGEIQTRVYPGVARGVEALHALGLKLAVVTNKPRSMASELLVRLGLARWIALVVGGDS
                     GLPRKPHPQPLLSACERLGVAPAQALMVGDSQIDVLAARAAGLAGVICVPYGYNEGVD
                     PRTLPCDRFVESIGDLPAVLGPRADRLVSAEPST"
     gene            complement(1083..>2328)
                     /locus_tag="VI195_02755"
     CDS             complement(1083..>2328)
                     /locus_tag="VI195_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6823335.1"
                     /translation="AIVGADYNDSSDTFTQSYQYGQFSADRMLVYQDSPFNNQTVISL
                     SGDNRIYGLYATDTLSPNALLHLTFSIRYNRSSETLDGYSVDTDIGDFGAGFDELRPL
                     AGDHTFSRVNPAFGFTLTPRETLTFYANYNEASRAPTVIELGCADPAVPCGLPNDFAS
                     DPALQQVVARTAEIGMRGSLAEQKLVWSADAFHTVNSNDIQFVATATNSGYFANVGST
                     RRQGIDLALGGRHAGWHWQLMYSLVDATFESSFTVNAASNSTADVNGDIVVRPGDRMP
                     LIPRHTGRLILDYQLGTRWNIGAQLVAASGSFLHGNENNANQAGATNASGAYIAGSGS
                     LPGYAVVNLQTTFRINEHAEVFARLVNLLDRHYATAGFLTSNSFNPDGSFIPDPANWT
                     NENALAPAQPLGIWAGLRVHLD"
BASE COUNT          372 a          724 c          837 g          395 t
ORIGIN      
        1 cgtggatgag ccgcccgaac aacatcgtcg tgcccggggg caagatgggc ctcgcgatcg
       61 agttcatttt cacgcccatg gtgttgcggc tgatggatcg caagcgccgc gcctagccga
      121 agcctgccgc atgcatgccg acagcaagac gcacacgccc cgcgcaggac cgcggtgggg
      181 tgccgtgcgg gcggtattgt tcgacctcga tggcacgatg ctcgataccg ccgcggacat
      241 caccgaggcg ctgaaccgcg cgctcgccga gcagggcctg ccgacgctcg cgcgcaacga
      301 agtgagcgag ctcattggcc ggggtgtgcc cgcactcata gagcgcgtgc tcgagcgttt
      361 cggtgcggct gcgagcaccg ccgatgccac gcggctgctc gagcgctttc accatcatta
      421 ccaatgcctg caccagcgcg gcgaaatcca gacgcgcgta tacccgggag tggcgcgggg
      481 cgtcgaggcg ctgcacgcgc tcggcctcaa gctcgcggtg gtgaccaaca agccgcgcag
      541 catggcgagc gagctgctgg tgcggctggg tctcgcccgg tggatcgcgc tggtcgtggg
      601 cggagatagc ggcctgccgc gcaagccgca tccacaaccc ttgctcagcg cttgcgagcg
      661 gctcggggtg gcgccggcgc aggcgctgat ggtgggggat tcccagatcg acgtgctggc
      721 ggcacgcgcg gcgggactgg cgggcgtgat ttgcgttccg tacggctaca acgaaggcgt
      781 cgaccctcga acgctgccgt gcgatcgctt cgtcgagagc atcggcgacc tgcccgcggt
      841 gcttgggccg cgagcagatc gcctcgtcag cgcagaacca tcgacttgaa gcgagccgcg
      901 ggcctcagcc ctgcggtcgc atgtcgctgc gcgcctgctg ctccgctctg cgtgcctcgg
      961 cacccgcatc gacatcgggc ttgagagcat ggatcgcacc ctcgagctgc tggccggaag
     1021 cgatctgcga cttcagatcc gcgaaccggc cagcgggccg ccgtgctcag gccggcgttc
     1081 gatcagtcga gatgcacgcg cagccccgcc cagatgccca gcggctgcgc cggtgcaagc
     1141 gcattctcgt tggtccagtt ggccgggtcg ggaatgaacg agccgtccgg gttgaaagag
     1201 ttgctggtga ggaatccggc cgtggcgtag tgccgatcga ggaggttgac cagtcgggcg
     1261 aaaacctcgg cgtgctcgtt gatgcggaaa gtggtctgga gattgaccac ggcgtagccc
     1321 ggcagtgagc cgctgccggc gatgtaagct cccgacgcgt tggtcgcgcc ggcctggttg
     1381 gcgttgttct cgttgccatg caggaacgag ccggaggcgg cgaccagctg ggcgccgatg
     1441 ttccagcggg tgccgagctg gtagtcgagg atcagccgcc cggtatggcg cgggatgagc
     1501 ggcatgcgat cgcccggacg aaccacgatg tcgccgttga cgtcggccgt gctgttggat
     1561 gcggcgttga ccgtgaagct cgactcgaag gtggcatcga ccaggctgta catcagctgc
     1621 cagtgccagc ccgcgtgccg gccgccgagc gcgaggtcga tgccctgacg gcgcgtgctg
     1681 ccgacgttgg cgaagtagcc ggagttggtg gcggtggcga cgaactgaat gtcgttgctg
     1741 ttgacggtgt gaaacgcgtc cgcactccac accagcttct gctcggcgag gctgccgcgc
     1801 atgcctatct cggcggtgcg cgccaccacc tgctgcagcg ccggatcgct ggcgaagtcg
     1861 ttgggcagtc cgcagggcac cgccggatcg gcgcagccga gctcgatgac cgtcggcgca
     1921 cgactcgcct cgttgtagtt ggcatagaag gtcagagttt cgcgcggtgt cagcgtgaag
     1981 ccgaaagccg gattgacgcg gctgaaggtg tgatcgcccg cgagcgggcg cagctcatcg
     2041 aagcccgcgc cgaaatcacc gatatcggta tcgacgctgt agccgtcgag cgtctcgctg
     2101 ctgcggttgt agcggatcga gaaggtgaga tgcagcagcg cgttcggcga gagcgtgtcg
     2161 gtggcgtaca gcccgtagat gcgattgtca ccgctgagcg agatcaccgt ctgattgttg
     2221 aacggactat cctggtagac cagcatgcgg tcggccgaga actgcccgta ctggtaggac
     2281 tgggtgaagg tgtcactcga gtcgttgtag tcggcgccca cgatcgcc
//
LOCUS       DATDMP010000166         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2137609, whole genome shotgun sequence.
ACCESSION   DATDMP010000166 DATDMP010000000
VERSION     DATDMP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137609"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VI195_02760"
     CDS             <1..724
                     /locus_tag="VI195_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEY6823336.1"
                     /translation="MILESRFPLMSPAWSPDAQWLAYVSFETKHAAVYVQLVRTGERR
                     QVSARAGVNGAPAWSPDGHKLALTLGGRSGNPDIYILDLSTQDLTRITDDPAIDTEPE
                     WAPDGKSLFFTSDRAGSPQIYQIGTNSGARPKRITFSGNYNARPRVSLDGTQLAMVTL
                     DNGNYRIAVLDLGSSTVRMLSHGHLDESPSFAPNGATLIYSERESGRGSLATVSTDGL
                     TGLRLKASQGEVREPAWGPFSP"
     gene            766..1329
                     /gene="pal"
                     /locus_tag="VI195_02765"
     CDS             766..1329
                     /gene="pal"
                     /locus_tag="VI195_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY6823337.1"
                     /translation="MSEKVSMHRVLAVLLLVAACALTACASKPSKPTGPGEGTANTGA
                     EGAAAGNPNANGSGNDDEVAGPQAGLLAKRTVYFDFDSSEIKGEGTDIVAAHAKYLAA
                     NPATRVRLEGHTDERGSREYNIGLGERRAQSVRRALLLQGAGDAQISTVSYGEERPAV
                     AGHDEAAWAKNRRVEIIYLAAGPAQKP"
     gene            1344..2108
                     /gene="ybgF"
                     /locus_tag="VI195_02770"
     CDS             1344..2108
                     /gene="ybgF"
                     /locus_tag="VI195_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY6823338.1"
                     /translation="MRVTLPALLTAMLAGCASTPPDKDPTQLRLNDLEARMERITANE
                     VQVSQSVDELQASLRQLRGQVEELEHNNDALRKQQRDLFGDLEKRLNAGGGGSAALAG
                     AAGGAAAGAAAGADSAAGTNAASPTEQAVYGQAFDALKAGSYSTAITGFKDFLRTYPN
                     SSLGDNAQYWLGEAYYVSHDYDSAAGAFRTVLKKWPDSQKAPDAMLKLGYTQLEQKQY
                     PAARTTLTEVTKKFPGTDSAKLAAERLRRIPAQASQ"
     gene            2157..>2346
                     /locus_tag="VI195_02775"
     CDS             2157..>2346
                     /locus_tag="VI195_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016711990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY6823339.1"
                     /translation="MTRLRITEIFHSLQGEADSVGIPTVFVRLTGCPLRCQYCDTTYA
                     FQGGEWWELPAILERVRAF"
BASE COUNT          419 a          767 c          798 g          362 t
ORIGIN      
        1 catgatcctc gagtcgcgct tcccgctcat gtcgcccgcc tggtcgccgg atgcacagtg
       61 gctggcgtat gtctccttcg agaccaagca cgccgcggta tacgtgcagc tggtgcgcac
      121 gggagagcgg cggcaggttt ccgcgcgcgc gggtgtgaac ggcgcgcccg cctggtcgcc
      181 cgatggccac aagctcgccc tgaccctcgg gggccgcagc ggcaatccgg acatctacat
      241 tctcgacctt tctacccagg acttgacgcg catcaccgac gatccggcga tcgataccga
      301 gcccgagtgg gcgccggatg gcaagtcgct gttcttcact tccgatcgcg ccggctcgcc
      361 gcagatctat cagatcggta ccaactccgg cgcgcgtccc aagcgcatca ccttcagtgg
      421 caactacaat gcccggccgc gggtgtcact ggatggcacg caacttgcga tggtgacgct
      481 cgacaacggc aactaccgca tagcggtgct ggatctcggc agcagcacgg tgcggatgct
      541 gtcgcacggt cacctcgatg aatcgccgag tttcgccccc aacggggcta cactcattta
      601 ttcggagcgc gaaagcggcc gcggctcgct ggcgacggta tcgaccgatg gcctgacggg
      661 tttgcggctg aaggcttccc agggagaggt tcgggagccg gcgtgggggc cgttcagccc
      721 ctgaagcggc gtcgcatgcg gcacgccgat tgcggaggac gtggaatgtc ggagaaggta
      781 tccatgcatc gagtgttggc cgtgctgctg ctcgtcgcgg cgtgcgcgct cactgcttgc
      841 gcgagcaagc cgtcgaagcc caccggcccc ggcgaaggca cagcgaatac gggcgcggaa
      901 ggagccgcag cgggcaaccc caacgcgaac ggttccggca acgatgatga ggtcgccgga
      961 ccgcaggcgg gcctgctcgc caagcgcacg gtgtacttcg actttgacag cagcgagatc
     1021 aagggcgagg gcacggacat cgtcgccgcg cacgccaagt acctcgcggc caatccggcc
     1081 acgcgggtgc gcctggaggg tcacacggat gagcgcggct cgcgcgagta caacatcggc
     1141 ctcggagagc ggcgcgcaca gtcggtgcgc cgggcgttgc ttttgcaggg cgcgggcgat
     1201 gcgcagattt ccacggtgag ttatggtgag gagcgtcccg cggtggcggg acacgacgag
     1261 gcggcctggg ccaagaatcg gcgcgtcgag atcatctacc tcgcggccgg gccggcgcag
     1321 aagccctagg gcgaaggatt gccatgcgtg taacactccc ggcgctgctc accgcaatgc
     1381 ttgcgggttg cgccagcaca ccccccgaca aggacccgac acagctgcgg ctcaatgacc
     1441 tcgaggcgcg catggagcgc atcaccgcca acgaggtaca ggtgtcgcag agtgtcgatg
     1501 agctgcaagc cagcctccgt caactgcgtg gacaggtcga ggagctcgag cacaacaacg
     1561 atgcgctgcg caagcagcag cgcgacttgt tcggcgatct cgaaaagcgc ctcaatgccg
     1621 gcggtggcgg tagtgcggca ctcgccggtg cggcgggggg cgcggccgcg ggagccgcgg
     1681 ccggtgccga cagcgcagca gggaccaacg cagcctcacc cacggaacag gccgtttacg
     1741 ggcaggcctt cgatgcgctc aaggcgggca gctactcgac tgcgatcacc ggcttcaagg
     1801 attttctgcg tacctatccc aacagctcgc tcggcgacaa cgcgcagtat tggctgggcg
     1861 aggcctacta tgtgagccac gactacgact ccgccgccgg tgccttccgc acggtgctga
     1921 agaagtggcc cgactcgcag aaggccccgg atgccatgct caagctcggt tacacgcagc
     1981 tcgagcagaa gcagtatccg gccgcgcgca cgaccctcac tgaggtgacg aagaagtttc
     2041 ccggaaccga ctcggcgaag ctcgccgcgg agcggctgcg ccgtatcccg gcgcaagctt
     2101 cccagtgagc gcctcggcgg tatacgccga gccgcacgtg atgccgcggt ccaagcatga
     2161 cacgcctcag gatcaccgag atcttccact ccctgcaggg ggaggccgat agcgtcggca
     2221 tcccgacggt gttcgtgcgc ctgacggggt gcccgctgag atgtcagtac tgcgacacca
     2281 cctacgcctt tcaggggggc gaatggtggg agttgccggc gatcctcgag cgggtgcgcg
     2341 ccttcg
//
LOCUS       DATDMP010000167         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2025778, whole genome shotgun sequence.
ACCESSION   DATDMP010000167 DATDMP010000000
VERSION     DATDMP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025778"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VI195_02780"
     CDS             complement(<1..411)
                     /locus_tag="VI195_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6823340.1"
                     /translation="MLDALRTLLRALVAQRSPSANASRLCADGEEACQHGRMEEGLRM
                     LREAVALSPEVPRFHYKLGVALQDAGELGAAADAYRRAIGLDADYAEAYNNLGCVQQL
                     QGDLDAALASYRRALSLKPSLPQASQNIAGLTGDT"
     gene            complement(411..2354)
                     /locus_tag="VI195_02785"
     CDS             complement(411..2354)
                     /locus_tag="VI195_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6823341.1"
                     /translation="MRRLCDAGSLRAPRPTRDTAASVKAEGNLLKAMGERGAAIARYR
                     RALELDGDYLPALYNLGLALHETGSYDEAEACFRRLVQAQPGDVDAWFHLGALLRRRM
                     QFKEAVATYERALEHAPDDPQAWLGLGDSILSTKDTKQIARAVSCYARAAELAPDLYA
                     AHFNRGCAAAMAGEHAEALLAFRAAAALHPEDPECLASLLWQMQQACDWSGFEDLCRR
                     RREVALTSQAPLDPFPLLTIASALPEQLASASAYARRIAQEALAGASRGALRRARRPG
                     SGKIRIGYLSADFHEHVTAHVMAEVFEKHRRDRFEIAAYSYGPDDGSAVRARLKGAFD
                     DFVELAREDDDAAAARIAADGIDVLIELKGYTRDARPGIAARRPAPVQASYLGYPGTM
                     GASFIDYIVTDRFVLPPEDQPFYKESPAYVRGCYYPSDSTRPVGDAPSRRELGLPEHE
                     LVFCCFNQSYKILPDVFAAWMRLLQAIPRSVLWLLESNRWANENLLREALRCGIDRER
                     LVISGRTSPTEYLRRLRAADLFLDTVPYNAHTTASDALWVGLPVLTCAGETLPSRVAA
                     SQLHALGLGDLVTTSLEHYLARAIQLARSSSELQAVRARVWRARSSSRLFDTVALCRD
                     LESLYEQMHRIAAQGAPPGPISA"
BASE COUNT          423 a          815 c          861 g          385 t
ORIGIN      
        1 cgtgtcgccc gtcaaccccg cgatgttctg gctcgcctgc ggcagggagg gtttcaggct
       61 cagggcgcga cggtagctcg ccagcgcggc gtcgagatcg ccttgcagct gctggacgca
      121 tccgaggttg ttgtaggcct cggcgtagtc ggcgtcgagg ccgatggcac gccggtaagc
      181 gtccgctgca gcgccaagct cgcccgcatc ttggagtgcg acaccgagct tgtagtggaa
      241 gcgcggaacc tccggcgaaa gagcgaccgc ttcgcgcagc atgcgcaggc cttcctccat
      301 gcgaccgtgc tggcaagcct cctcgccgtc ggcgcacaaa cgcgaggcat tcgcgctggg
      361 cgagcgttgc gcgacgagag cgcgcagcag ggtgcgcagt gcgtcgagca tcaggccgag
      421 attggccctg gcggcgcgcc ctgcgccgca atgcgatgca tctgctcgta gagggattcg
      481 agatccctgc acaaggcaac ggtgtcgaaa agcctcgagc tggaccgcgc ccgccagacg
      541 cgcgcgcgca ccgcctggag ctccgaggac gagcgcgcaa gctggatggc gcgcgcgagg
      601 taatgctcca gagacgttgt gaccaggtct ccgaggccga gcgcgtgcag ctggcttgcc
      661 gccacacggg atgggagcgt ctcccccgcg caagtgagca ccggaaggcc gacccagagc
      721 gcatcgctcg cggtggtatg cgcgttgtac ggcacggtat ccaggaaaag gtccgcagcg
      781 cgcagccgcc ggaggtattc ggtcggggac gtcctgcccg agatcaccag ccgctcacgg
      841 tcaatgccgc atcgaagcgc ttcacgaagc agattttcgt ttgcccagcg gttgctctcg
      901 agcagccaaa ggaccgatcg cggtatcgcc tgcagcagcc gcatccaggc cgcgaagacg
      961 tcgggcagga ttttgtagct ctggttgaag cagcagaaga cgagctcgtg ctccggaagg
     1021 ccgagctcgc ggcgcgatgg cgcatcgccg accgggcgcg tggaatcgct cgggtagtag
     1081 cagccgcgga catacgccgg cgactctttg tagaacggtt ggtcctcggg cggaagcacg
     1141 aagcggtcgg tgacgatgta gtcgatgaac gaagcgccca tcgtgccggg gtagccgagg
     1201 tagctcgcct gaaccggtgc cggcctgcgg gccgcgatgc cagggcgagc gtcgcgcgta
     1261 tagcccttca gctcgatcag aacgtcgatg ccgtcggctg cgatgcgcgc agcggctgcg
     1321 tcgtcgtcct cgcgggcaag ctcgacgaaa tcgtcgaaag cgcccttcag ccgcgcccgg
     1381 accgcgctgc cgtcatcggg accgtaggaa taggccgcga tctcgaatct gtcccgccgg
     1441 tgcttctcga agacctcggc catgacgtgg gccgtgacgt gctcgtggaa gtccgccgag
     1501 aggtacccga tcctgatctt gccggagccc gggcgcctgg cacgccggag ggcgccgcgt
     1561 gaagcgccgg cgagcgcttc ttgcgcgatc cggcgggcat aggcgctcgc cgacgcgagc
     1621 tgctccggca aagcggacgc aatggtgagg agcgggaagg gatccagcgg cgcttgcgac
     1681 gtgagcgcca cctccctgcg tctgcggcac aggtcttcga atcctgacca gtcgcaagcc
     1741 tgctgcatct gccatagcaa cgaagcaagg cattcggggt cttcaggatg cagcgcagcc
     1801 gcggcgcgga atgcgagcaa ggcctccgcg tgctccccgg ccatggcggc cgcgcatccc
     1861 cgattgaagt gcgctgcgta cagatcgggc gccagctctg ccgcccgcgc atagcaactc
     1921 actgccctag cgatctgctt ggtgtccttc gtggagagaa tgctgtcgcc gaggccaagc
     1981 caggcctgcg ggtcatccgg ggcgtgctcc agcgcacgct cataggttgc gaccgcttcc
     2041 ttgaactgca tccggcgccg caacagggcg ccgagatgga accaagcatc cacgtcgccc
     2101 ggctgcgctt gcaccagccg gcggaagcaa gcctcggcct cgtcgtagga gccggtttcg
     2161 tgaagcgcaa gacccaggtt atacagcgca ggaaggtagt cgccgtcgag ctcgagcgcg
     2221 cggcggtagc gtgcgatggc cgcgccgcgc tcgcccattg ccttgagaag attcccctcc
     2281 gccttgacgg aggcagcggt gtcccgcgtc ggccttggtg ctcttaaaga acctgcgtcg
     2341 caaagccggc gcagccagga aacccaccct tcatccggag catgtgcaga tcgcgcgact
     2401 gcatcccacg aagaatcgtc ggcggattgc ggcccgcccg gggcggtatc cgcctcttcg
     2461 cgccaaagga cgagccgggc ggcg
//
LOCUS       DATDMP010000168         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1137108, whole genome shotgun sequence.
ACCESSION   DATDMP010000168 DATDMP010000000
VERSION     DATDMP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137108"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VI195_02790"
     CDS             <1..667
                     /locus_tag="VI195_02790"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF000734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY6823342.1"
                     /translation="RGPLEPADRHGISRMNLPRVYPIVDCAAWIAPLAAAGVRLVQLR
                     IKEQPEERVRLEIESARVHCQAAGVQLIINDYWRLALGCGCDFVHLGQGDLDGADLPA
                     LRRAGVRLGISTHDEAELERALALGPDYVALGPVYPTLLKTMPWRPQGLARVTEWKRR
                     VGAIPLVAIGGITLERLPGVFAAGADVAAVVTDIVRASNPEERVRQWLAVAGAVRRER
                     LSA"
     gene            686..1660
                     /locus_tag="VI195_02795"
     CDS             686..1660
                     /locus_tag="VI195_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEY6823343.1"
                     /translation="MSDRYARQEILPEVGAEGQARLGAASALIVGAGGLGCAVLQYLT
                     AAGLGRLIIVDHDRVEESNLHRQPLYRMGDVGQLKVEAARAALAALNPGTHIEAVCER
                     LSSINAARLARAANVVIDAADSFAVTYLLSDTCQRLGKPLVSASVLGLRGYVGVFCGG
                     APSYRAVFPELPGVAGSCAESGVLGTAVGVLGTLEAHLALALLLDWQPAVRGRMLSLD
                     LRTLHVGGFSFAAAREPMETPIPFIAPAEVADNDVVIDLRSLSEAPLSPIQHALRIEV
                     EALERALLEFPGEARIVLCCRSGLRAWRAARTLRSRGHEKLALLALGE"
     gene            1657..>2132
                     /locus_tag="VI195_02800"
     CDS             1657..>2132
                     /locus_tag="VI195_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEY6823344.1"
                     /translation="MTTVLVIAGSDSSGGAGVTRDVHTLTQLGVQARCVVTAVTAQSD
                     ASVYAVHVLPAELVRAQLAAACATGPVDAIKIGMLARRATVLAVCAAIPAGALPIVLD
                     PVLASSSGGELLDAQGREALRTTMLPRATLLTPNIPEAAALLGRDCAHSEAELLQQA"
BASE COUNT          293 a          715 c          768 g          356 t
ORIGIN      
        1 tcgcggtccc ctcgagcccg ctgatcggca cggcatttcg cggatgaacc tgccgcgcgt
       61 ctatccgatc gtcgactgcg ccgcctggat cgcgccgctg gcagcggcgg gggtgcggct
      121 ggtgcagctg cgcatcaagg agcagcccga ggagcgggtg cggctcgaga tcgagagcgc
      181 ccgcgtccac tgccaggccg cgggcgtgca actgatcatc aacgactact ggcggctggc
      241 gctcggctgc ggctgcgatt tcgtgcacct cggacagggc gatctcgatg gagcagatct
      301 gccggcgctg cgccgcgcgg gtgtgcggct cggcatcagc acgcatgatg aggcggagct
      361 cgagagggcc ctcgcgctcg gcccggacta tgtggctctc ggcccggttt atccgacact
      421 gctcaagacg atgccctggc ggccgcaggg acttgcgcgc gtcacggagt ggaaacgccg
      481 cgtcggcgcg atcccgctcg tggcgatcgg cggcatcacc ctcgagcggc tgcccggggt
      541 attcgccgcc ggcgccgatg tggctgcggt cgtgaccgac atcgtgcgcg cgagcaaccc
      601 ggaagagcgc gtgcggcagt ggctcgcggt ggccggcgcg gtgcggcgcg agcgcctgag
      661 tgcctgaaac cgccacggtg tcagcatgag tgatcgctat gcgcggcagg agatccttcc
      721 cgaggtcggt gctgagggtc aggcacggct tggcgcagcg agcgcgctca tcgtcggtgc
      781 cggcggtctc gggtgcgccg tgctgcagta tcttacggca gcggggctcg gcagactgat
      841 cattgtcgat cacgatcggg tggaggagtc gaaccttcac cgtcaaccgc tctatcgcat
      901 gggcgacgtc gggcaattga aggtcgaggc ggcgcgcgct gcgctcgccg ccttgaatcc
      961 tggcacgcac atagaggccg tgtgcgagcg gttgagctcg atcaatgccg cacgcctcgc
     1021 tcgggccgcg aacgttgtga tcgacgccgc cgacagtttc gcggtcacct acctcctgag
     1081 tgatacctgt cagcgcctgg gcaaaccgct cgtgagtgcc tcggtgctcg ggctcagagg
     1141 ctacgtcgga gtgttctgtg gtggcgcgcc gagctaccgc gcggtctttc cggagctgcc
     1201 cggcgttgcc ggctcgtgtg ccgagtcggg cgtgctcggc accgcggtcg gggtgctggg
     1261 tacgctcgag gcgcatctcg cattggcgct gctgctcgac tggcagcccg cggtgcgcgg
     1321 acgcatgctg tcgctcgatt tgcgcacgct gcacgtgggc ggcttctcct tcgccgcggc
     1381 gcgcgagccg atggagacgc ccattccctt catcgcaccc gccgaggtag cagacaacga
     1441 tgtcgtgatt gatctgcgca gcctcagcga ggcgccgctg tcacccatcc agcacgcgct
     1501 gcgtatcgag gtcgaggcgc tcgagcgagc gctgctcgag tttcccggcg aagcgcgcat
     1561 cgtgctctgc tgccgcagcg gactgcgcgc ctggcgcgcc gcgcgcacgc tgcgcagccg
     1621 cggacacgag aaactcgcgc tgctcgcgct cggcgagtga ctacggttct cgtcatcgcc
     1681 ggctccgact ccagcggcgg agcgggagtg acgcgcgatg tgcacaccct gacgcagctc
     1741 ggggtgcaag cgcgctgcgt ggtcacggcg gtgaccgcgc agtcggatgc aagcgtttac
     1801 gcggtgcacg tgctgcctgc cgagctggtg cgcgcgcagc tcgccgcagc ttgcgccacc
     1861 ggccccgtcg atgcgatcaa gatcggcatg ctggcgagac gcgcaaccgt gctcgccgtc
     1921 tgcgccgcta ttccggcagg agcattgccc attgtgctcg atccggtgct ggcgtcctcc
     1981 tccggcggcg agctactcga tgcgcagggg cgggaagccc tgcgtaccac gatgctgcca
     2041 cgcgccacgc tgctgacgcc caacatcccg gaggccgcag cactgctcgg ccgcgattgc
     2101 gcgcacagcg aggcggaatt gctgcagcag gc
//
LOCUS       DATDMP010000169         4116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_248933, whole genome shotgun sequence.
ACCESSION   DATDMP010000169 DATDMP010000000
VERSION     DATDMP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248933"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VI195_02805"
     CDS             <1..380
                     /locus_tag="VI195_02805"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY6823345.1"
                     /translation="GNFMLAATIETLGEALTLVGKGGIEPGAFVELLASTLFPAPAYR
                     TYGALIAESKFQPAGFAAPLGHKDVRLLLAAAESLRVPMPIGSLLNDRFLRLLAQGGE
                     NLDWSAISQLAAADAGLPRISRS"
     gene            507..1016
                     /locus_tag="VI195_02810"
     CDS             507..1016
                     /locus_tag="VI195_02810"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY6823346.1"
                     /translation="MDEAIQEKILQLLDQHRIMTIATLRADGWPQATTVGYANEGLRL
                     YFLCGLDSQKATNIARDNRVSLTIDHDTPQVMEITGLSMAARAQVVDDPGEGEKALGL
                     LMQKYPPQLSLPFPMPKFAEVRIFRVIPEVISLLDYSKGFGHTDLISCRAPHAAPGAI
                     PLTASSHAM"
     gene            1016..1144
                     /locus_tag="VI195_02815"
     CDS             1016..1144
                     /locus_tag="VI195_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823347.1"
                     /translation="MKYQAYRRGAPTGTRHDHELTGWHDLAPFAGTFIAAQAVTRA"
     gene            1167..2132
                     /locus_tag="VI195_02820"
     CDS             1167..2132
                     /locus_tag="VI195_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY6823348.1"
                     /translation="MLLLAAGCLAGSTAQPLKLQPLADVPLGGHTTRLDYASLDPRRH
                     LLFIAHLGDGVLIVFDTETQRVSRRIAGLSKVHGVLAIPELDTVYASATGTNEIVAID
                     EGTLTVSARMPGGVYPDGIAYAPAVHKLYVSDELGRTETVIDVRTHQRVATIPLGGAV
                     GNSQYDPVTQHIFVNVQTLSQLIEIDPVTDRITARTPLPGAQSNHGLLIEPQQRRAFI
                     ACEDNHKLLLLDLDSRKVTASFEVGVGPDVLAYDPGLKYLYVAAESGIVSTFNASSGE
                     ITPVGSGLIGPNAHVVAVDPATHRVFFPLRNIGGRPVLRIMSPGT"
     gene            2129..3841
                     /locus_tag="VI195_02825"
     CDS             2129..3841
                     /locus_tag="VI195_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HEY6823349.1"
                     /translation="MIRAPISRRRFVQGIVGGAMLLAPLRSFPGSTAERGRSVLMGPD
                     FDLTIGETAVNFTGRSRTATAVNGLVPAPLLRMRQGDAVTIRVTNKLPVRSSIHWHGM
                     LLPADMDGVPGLSFAGIDPGATFVYRFKLRQSGTYWYHAHSRFQEQTGLYGPIVIDPA
                     GGERHGSDREYVLLLSDWSDADPEWLYATLKRQSNYFNYGKRTAGDFFAEVRARGLHA
                     ALAQRSMWGKMRMDPTDLADVGGAAYTYLINGVTPAGNWTALFRRGERVRLRVINGSS
                     MSFFDVRIPGLKLTVVAADGQDVEPVTVDEFRIGAAEVLDVMVTPSAEQAYTIFAQSM
                     DRSGYARGTLAPQPGMAAEVPPLEPRGLLAMHDTGMGHAMPAAGDMPGMSGMEHSTRA
                     PAEFGPTVDMVAAQPSVRLDDPGPGLRANGRRVLSYADLHTLGGPIDRRDAGRDIELH
                     LTGHMERFIWSFDGQKFSEAQPLRFVLGERLRIVLVNDSMMTHPIHLHGMWSEVEAPG
                     GAFLVRKHTVSVQPAQRLSYRVTADAPGRWAYHCHLLYHMQAGMFREVVVGGPGAVAG
                     GEHA"
     gene            3874..>4116
                     /locus_tag="VI195_02830"
     CDS             3874..>4116
                     /locus_tag="VI195_02830"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEY6823350.1"
                     /translation="MALLLHAAAAPAQEAAAPASAPTELAGMSHRDMTRLMEMDDATA
                     FGKLVADQFEWRTGDGTAGPAWDVQGWYGGDYSKLWF"
BASE COUNT          707 a         1367 c         1338 g          704 t
ORIGIN      
        1 cgggcaactt catgctcgcc gcgaccatcg agacactcgg tgaagcgctc acgctcgtcg
       61 gcaaaggcgg tatcgagccg ggcgcgttcg tcgagctgct ggcatccacg ctgtttcccg
      121 cgccggctta ccgaacctac ggcgcactga ttgcagagag caagttccag ccggcgggct
      181 tcgcagcgcc gctcggacac aaagatgttc gcctgctgct cgcggcggcg gaatcgctgc
      241 gcgttcccat gcccatcggc agtctgctga atgaccgctt cctgcgcctg ctcgcgcagg
      301 gaggcgagaa cctggactgg tccgccatca gtcagcttgc ggccgcagat gccgggctgc
      361 cccgcatcag ccgttcgtga agtgcttacc gaatcgcgtg atccatccag gtatgtgttt
      421 gcgcgtatgg acccctgact gccgccggtg gcagtgtgac gtcctgcgcg aggctacctg
      481 cccgagcgct ggccggatga tggtcggtgg acgaagcgat acaagagaag attctgcaac
      541 tgctcgatca gcaccgcatc atgacgatcg cgacgctgag ggcggacggc tggccgcaag
      601 ccactacggt cggttacgcg aatgagggcc tgaggctgta ctttctgtgc gggctggaca
      661 gtcagaaagc gacgaacatc gcgagagaca atcgggtctc gttgacgatc gatcacgaca
      721 cgccacaagt gatggaaatc accgggctct cgatggctgc ccgggcgcag gtggtcgacg
      781 atccgggcga aggggaaaag gcgctgggcc tgctcatgca gaagtatcca ccgcagctct
      841 ccctgccctt ccccatgccg aagttcgccg aggtgcgcat atttcgggtg atacctgagg
      901 taatttcgct gctcgactat tccaaaggct tcggtcacac cgatctcatc agctgccggg
      961 cgccgcatgc ggcgcccggc gctataccgc ttacggcttc ctcccacgcc atgtgatgaa
     1021 ataccaggcc tatcgcaggg gtgcccccac cggcacccgg cacgaccacg agctgacggg
     1081 ctggcacgat cttgcgcctt tcgccggtac attcatcgcc gcccaggcgg tcacgcgcgc
     1141 gtaggagctg gaaactcgct catccgttgc tgctactggc cgccggctgt ctcgccggca
     1201 gcaccgccca acccctgaag cttcagccgc tggccgatgt gccgctcggt ggccacacga
     1261 cgcgcctcga ctacgcgagt cttgaccctc gccggcacct gctgttcatc gcccatctcg
     1321 gcgacggtgt cctcatcgtg ttcgacaccg agacacagcg ggtcagccgc cgcatcgcag
     1381 gcctcagcaa ggtgcacggc gtgctggcca tcccggagct cgacaccgtg tacgcctccg
     1441 ctacgggcac caacgagatc gtcgccatcg acgaaggcac gctcacagtc agcgcgcgca
     1501 tgcccggggg ggtatatccc gatggcatcg cgtatgcgcc cgcggtccac aaactgtatg
     1561 tgtccgatga gctcggccgt accgaaaccg tgatcgatgt gcgcacccac cagcgcgtcg
     1621 cgacgatccc gctgggtggc gcggtcggca acagccagta tgatccggtc acccagcata
     1681 tcttcgtcaa cgtgcagacc ctcagccagc tcatcgagat cgatcccgtg acagaccgga
     1741 tcaccgcccg cacgccactg ccgggagcgc agagtaacca cggcctgctg atcgagccgc
     1801 agcagcggcg cgcgttcatc gcctgtgaag acaatcacaa gctcctcctg ctcgacctcg
     1861 acagccgtaa agtcaccgct tcctttgagg tcggtgtggg ccccgacgtg ctcgcgtacg
     1921 acccgggcct caagtatctc tacgtcgcgg ccgagtcggg gatcgtgtcg acattcaacg
     1981 catcatccgg cgagatcacc ccggtgggca gcggcctcat cggccccaac gcgcacgtgg
     2041 tggcagtgga tccggcaacg cacagggtgt tcttcccgct gcgcaacata ggcgggcgac
     2101 cggtgctacg gatcatgagt cccggcacgt gatacgagcg ccgatttcgc ggcgccggtt
     2161 cgtacaggga atcgtcggtg gggcgatgtt gctcgctccg ctgcgctcct ttccgggcag
     2221 caccgccgag cgcggacgct cggtcctcat gggccccgat ttcgatctca cgatcggcga
     2281 aaccgcggtc aacttcaccg gccgcagccg taccgccacc gcggtcaatg gtctcgtgcc
     2341 ggcgccgctg ctgcgcatgc gccagggaga cgcggtcacg atccgcgtca ccaacaagtt
     2401 gccggtgcgc agctccattc actggcacgg catgctgctc ccggccgata tggacggcgt
     2461 gccgggattg agctttgccg gcatcgaccc cggcgctacc ttcgtctatc ggttcaagct
     2521 gagacagtcc ggcacctact ggtaccacgc gcactcgcgc ttccaggagc agaccggcct
     2581 ctacggccca atcgtcatcg acccagcggg cggggagcgc cacggcagcg atcgggaata
     2641 cgtgctgctg ctgtcggact ggagcgatgc cgacccggag tggctgtacg cgacactcaa
     2701 gcggcagagc aactacttca actacggcaa gcgcacggcc ggagatttct tcgccgaggt
     2761 tcgcgcgcgc ggtctgcacg cggcgctcgc gcagcggagc atgtggggaa agatgcgcat
     2821 ggatccgacc gacctggccg acgtcggtgg cgctgcctac acctacctca tcaacggcgt
     2881 aacccccgcg ggcaactgga ccgcgctgtt ccgccgcggc gagcgcgtgc gcctgcgcgt
     2941 gatcaacggt tcctcgatga gttttttcga cgtacggatc ccagggctga aactcacggt
     3001 ggtggcggcg gatggccagg acgtcgagcc ggttaccgtg gatgagtttc gtatcggcgc
     3061 cgccgaagtg ctcgatgtga tggtgacgcc gagcgccgaa caggcctaca cgatcttcgc
     3121 gcagtcgatg gaccgctcgg gctacgcgcg cggtacgctc gcgccacagc ccggaatggc
     3181 ggccgaggtt ccgccgctcg agccgcgcgg gctcctcgcg atgcatgaca ccggcatggg
     3241 acacgctatg ccagcggcag gggacatgcc gggaatgtcc ggcatggagc actcgacgcg
     3301 cgcacctgcc gaattcgggc cgacggtcga tatggtggcg gcgcaaccca gcgtgcgtct
     3361 cgatgatccc ggtccggggt tgcgcgctaa cggccgccgc gtgctgagct atgcggattt
     3421 gcatacgctc ggcggcccga tcgatcggcg cgatgcggga cgggacatcg agctgcatct
     3481 caccggccac atggagcgat tcatctggtc cttcgacggg cagaaattct ccgaggcgca
     3541 gccgctgcga ttcgtgctcg gtgaacggct gcgcatcgtg ctggtgaacg attcgatgat
     3601 gacgcacccg atccacctgc acggcatgtg gagcgaggtc gaagcgcccg gcggtgcctt
     3661 cctcgtgcga aaacacaccg tcagcgtgca gcccgctcag cgcctgagct atcgggtgac
     3721 ggccgacgcc cccgggcgct gggcatatca ctgccacctg ctctaccaca tgcaggccgg
     3781 gatgtttcgt gaggtagtag tagggggccc gggtgcagtc gctggcggag agcatgcgtg
     3841 aggctcgctc cggtcctgac agcgagcggg tccgtggctc tgctgctgca tgctgccgca
     3901 gcacctgcgc aggaagcggc ggccccggcg agcgcgccga cggagttggc ggggatgtct
     3961 caccgcgaca tgacgcggct catggaaatg gacgatgcca cggcgttcgg caaactcgtc
     4021 gccgaccagt tcgagtggcg cacgggcgat ggtacggctg gaccggcctg ggacgtccaa
     4081 ggatggtacg ggggcgatta cagcaagctg tggttc
//
LOCUS       DATDMP010000170         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1360514, whole genome shotgun sequence.
ACCESSION   DATDMP010000170 DATDMP010000000
VERSION     DATDMP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360514"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VI195_02835"
     CDS             <1..582
                     /locus_tag="VI195_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY6823351.1"
                     /translation="WHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCMSTL
                     HANSTNQALDRIINFFPDERREQLLMDLSLNIRALISQRLIPRESGAGRIAAMEIMLN
                     SPLIQDLIFKGEVAKIKEVMSRSTRLGMKTFDQTLFELYETGFISYEDALRNADSKNE
                     LRLRIKLEGKREAKNIEEGAALHIVEEEQGRTL"
     gene            582..1745
                     /locus_tag="VI195_02840"
     CDS             582..1745
                     /locus_tag="VI195_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY6823352.1"
                     /translation="MKAVKEAKEAPETNGVQAVLGGAETLATVSGGSHPTLNTKPLFK
                     LMVEKKASDLFFTSNAPIKIKIEGQILPVNKQVLTPETVRQTAFAIMSAEQREHFQRE
                     LELDFAVSEAGLGRFRVNVFMQRGYPAMVMRYISADMPRLDTLGLPPTVGELTLLKRG
                     LILMVGATGSGKSTTLAAMINHRNESSSDHIITIEDPIEYLHTNKRSIVNQREVGLDT
                     RSYTGALRSVVRAAPDVILIGEIRDRESMEAAVNLAGTGHLVLATLHANNCAESLDRI
                     INMFPREQHNQIFLDLSQYLRAVMAQRLVMGKDTRRLAAMEIMINTPHISTLVKKGDV
                     VSIKEAISASSERGVQSFDVALFALYKAGRIPLEEALANADSRANLEAKINFG"
     gene            complement(1878..>2305)
                     /locus_tag="VI195_02845"
     CDS             complement(1878..>2305)
                     /locus_tag="VI195_02845"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823353.1"
                     /translation="ARPSTLLVPGIPARATACMPLLEEWGVPSRLASGADFPGCFAGF
                     VTELAAAWLESLGPGELGEVEIFACGPAPMLAATARLARRYGVPCQVSLEEFMACAVG
                     GCAGCTVRVRTSEGEAMRRVCVDGPVFDAYTVFADPGTG"
BASE COUNT          444 a          817 c          721 g          323 t
ORIGIN      
        1 tggcatcacg cgctcaagaa cacgctgcgg caggcacccg acgtaatcct cattggcgaa
       61 atccgcgatg cggaaacgat ggagcacgcc atcgcttttg cggaaacggg ccacctgtgc
      121 atgtccacgc tgcacgcgaa ctccaccaac caggcgctcg accgcatcat caacttcttc
      181 ccggacgagc gccgtgagca gctcctgatg gacctctcgc tcaacatccg cgcgctcatc
      241 tcgcagcgcc tgatcccgcg cgaatccggc gccggccgca tcgcggccat ggagatcatg
      301 ctcaactcgc cgctgatcca ggacctgatc ttcaagggcg aggtcgccaa gatcaaggag
      361 gtcatgtcgc gctcgacgcg tctcggcatg aagaccttcg accagacgct gttcgagctg
      421 tacgagaccg gcttcatctc ctacgaggac gcgctgcgca acgccgactc gaagaacgag
      481 ctgcggctgc gcatcaagct cgagggcaag cgtgaggcca agaacatcga ggaaggcgct
      541 gcgctgcaca tcgtcgagga agagcagggg cgcacgctct gatgaaggcg gtgaaggaag
      601 ccaaggaggc tccggagacc aacggggtcc aggcggtgct cggcggcgcc gagacgctcg
      661 ccacggtgag cggcggcagc cacccgacgc tcaacaccaa gccgctgttc aagctgatgg
      721 tggaaaagaa ggcttcggac ctgttcttca cctccaacgc tcccatcaag atcaagatcg
      781 aggggcagat cctgccggtc aacaagcagg tgctcactcc cgagacggtg cgccagactg
      841 cctttgcaat catgagcgcc gagcagcgcg agcacttcca gcgcgagttg gagctcgact
      901 tcgcggtgtc cgaggccggc ctcgggcgct tccgcgtcaa cgttttcatg cagcgcggct
      961 acccggcgat ggtgatgcgc tacatcagcg ccgacatgcc gcggctcgac acgctgggac
     1021 tgccgcccac ggtgggcgag ctgacgctgc tcaagcgcgg cctgatcctc atggtgggcg
     1081 ccaccggctc gggcaagtcg accacgctcg ccgcgatgat caatcaccgc aacgagagct
     1141 cctcggacca catcatcacc atcgaggacc cgatcgagta cctgcacacc aacaagcgct
     1201 cgatcgtcaa ccagcgcgag gtgggcctgg acaccaggtc ctacaccggc gccctgcgca
     1261 gcgtggtgcg tgccgccccc gatgtgatcc tgatcggcga gatccgcgac cgcgagagca
     1321 tggaggcggc cgtcaacctc gccggaaccg gccacctggt gctggctacc ctgcacgcca
     1381 acaactgcgc cgagtcgctc gaccgcatca tcaacatgtt cccgcgcgag cagcacaacc
     1441 agatcttcct cgacctgtcg cagtacctgc gcgccgtcat ggcgcagcgc ctggtgatgg
     1501 gcaaggacac ccgccgcctc gccgccatgg agatcatgat caacaccccg cacatctcga
     1561 cgctcgtcaa gaagggcgac gtggtgtcga tcaaggaagc gatctccgcg agcagcgagc
     1621 gcggggtgca gagcttcgac gtcgccctgt tcgcgctcta caaggccggc aggatcccgc
     1681 tcgaagaggc gctggcgaac gccgactcgc gcgccaatct cgaggccaag atcaacttcg
     1741 gctgagagac ccccgccgca ccgcaaccgc agccgccggc gggcggcatc gtgggactcg
     1801 acgactgagc ggctgcgcgt ggagtaacgg tcccgtccgc gatagttaag tggttgctta
     1861 actatcgcga agggacacta tccagtcccc gggtccgcga acaccgtata ggcgtcgaac
     1921 accgggccgt cgacgcacac ccgcctcatc gcctcgccct cgctcgtgcg cacccgcacc
     1981 gtgcagccgg cgcagccgcc caccgcgcag gccatgaatt cctcgagcga tacctggcag
     2041 ggcacgccgt agcgccgcgc gaggcgcgca gtcgccgcca gcatcggcgc cggcccgcag
     2101 gcgaagatct cgacctcccc gagctcgccc ggcccgagcg actcgagcca cgccgcggcg
     2161 agctcggtga cgaagcccgc aaagcacccc gggaagtccg cgccgctcgc gagccggctc
     2221 ggcacgcccc actcctcgag cagcggcatg caggcggtgg cgcgtgccgg gattcccggg
     2281 acgaggagcg tcgagggccg cgcgc
//
LOCUS       DATDMP010000171         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1693841, whole genome shotgun sequence.
ACCESSION   DATDMP010000171 DATDMP010000000
VERSION     DATDMP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693841"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VI195_02850"
     CDS             <1..456
                     /locus_tag="VI195_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823354.1"
                     /translation="EAAGRFFQAGTALAALCRMKILCAVLLAAASAASFAQGTASTGG
                     IGAAPGAPMSGAPIGNAGTYGTTPGTGIGGAPTGNTGTFGTTPSNTGVGAPTGASSTF
                     GTPLPGTGAVTTPGRTTFDNVLNSGSSTGSSAASGATQATTCRPGAIVC"
     gene            503..590
                     /locus_tag="VI195_02855"
     tRNA            503..590
                     /locus_tag="VI195_02855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:537..539,aa:Ser,seq:tga)
     gene            606..1439
                     /locus_tag="VI195_02860"
     CDS             606..1439
                     /locus_tag="VI195_02860"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEY6823355.1"
                     /translation="MSIEKRFAVIGFGAITEEILRCLAQLGASQSLVGVLVRPERLAE
                     ARNKAGGRFPVVDRLDALLELRPDAVAECAGHGAMRQFGAEVLARGTDLLCASVGVLA
                     DRDFAAALMRQARGRLLIPSGAVAGLDGLLAARTAGLERVTYTSVKPPAAWIGTPGEA
                     QVAAARGKRIAFFEGTAREAALQYPQNANVGMAVSLAGLGPDRTIARLVSDPEASGPL
                     GIIEAEGDFGRFRFEILAYASRSNPKTSALTAHSVVAALREGMAFEVDRLDAEKNKGV
                     H"
     gene            1477..1788
                     /locus_tag="VI195_02865"
     CDS             1477..1788
                     /locus_tag="VI195_02865"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY6823356.1"
                     /translation="MGWHEIPEAASLPDEEARAVVIGQRQIALCRSGGTLHAIDNVCT
                     HQYALLSEGYVEDGCIECPLHQGRFDLKTGTPQGAPVTEPVRVYPLRTEGGKTYVELP
                     D"
     gene            1794..>2887
                     /locus_tag="VI195_02870"
     CDS             1794..>2887
                     /locus_tag="VI195_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6823357.1"
                     /translation="MPLERIVIIGAGQAGGRAAEALRSAGFKGEVLLLGDEAHEPYER
                     PALSKGVLLGEETAARTLIHPREWYAAQKIDLRTATRVEAIAPVSQTVITAQGERIEY
                     SRLLLCTGSRVRPLAGANESLRGVHYLRTIGDSERLAAELHPGRRIVIIGGGFIGLEV
                     ASSAAKRGLAVRLVERQAALLDRALPAEIAALVESFHRSQGVQLHLGVPVARIHGREA
                     VESVELADGTNLDADLVVVGIGIIPNTELAEAAGAASSDGIVVDEYCRTNVANLYAAG
                     DATSHYNPLLERRVRLESWQNAQNQAVAAAHNLSGNARPYAEIPWFWSDQFGRNIQMV
                     GIAEPGLEVSWRGEPRSGRCMAFGLRDGRVR"
BASE COUNT          460 a         1016 c         1012 g          399 t
ORIGIN      
        1 gaggccgcag ggcggttttt tcaagccggc acggcacttg cggcgctgtg ccgcatgaag
       61 atcctctgcg cggtactgct cgcggcagcc tccgccgcct ccttcgcaca aggcacggcc
      121 agtaccggcg gcatcggcgc cgcccccggc gctcccatga gcggggcacc gatcggcaac
      181 gctgggacct acggtaccac gccaggcacc ggcatcgggg gcgctccgac gggcaacacc
      241 ggcaccttcg gcacgacgcc cagcaacacc ggcgtcggcg cgcccaccgg cgcgagcagc
      301 acgttcggca cgccgcttcc cggaaccggc gcggtgacca cgccgggccg caccactttc
      361 gacaacgtcc tgaacagcgg ctcctccacc ggcagcagcg ccgcgagcgg cgcgacgcag
      421 gccaccacct gccgtccggg cgcgatcgtc tgctgaaggg gcggcaagag gggcggaccc
      481 cttccgccgt aaaatcccgc tcggagagtt ggcagagcgg tcgaatgcgg cggtcttgaa
      541 aaccgctagc ccgcaagggc tcgggggttc gaatccctca ctctccgcca cagcccggcg
      601 ggcccatgag catcgaaaaa cgattcgcag tcatcgggtt cggcgcgatc accgaggaaa
      661 tcctccgttg cctcgcgcag ctcggagcct cgcaaagtct cgtcggcgtc ctggtgcgcc
      721 cggaacggtt ggccgaggca cgtaacaaag ccggcggcag atttccggta gtcgatcgtc
      781 tcgacgcgct tctcgagctg cgcccggatg cagtcgccga atgcgcgggg cacggcgcga
      841 tgcgccagtt cggagcggag gttcttgcgc gcggcacgga cctcctgtgc gcctcggtag
      901 gcgtccttgc cgatcgagat ttcgccgccg cattgatgcg ccaggcgcgc gggcgacttc
      961 tgattccttc gggcgcggtc gcgggactcg acggcctgct cgcggcgcgc acggccggcc
     1021 ttgagcgcgt gacgtacacc tcggtgaagc cgcccgcggc ctggatcgga acccccggcg
     1081 aggcgcaggt ggccgcggcg cgcggcaagc gcatcgcgtt ttttgaaggc acggcgcgcg
     1141 aagcagccct gcaatacccg cagaacgcga acgtcgggat ggcggtgtcc ctggcggggc
     1201 tcggcccgga ccggacgatc gcgaggctgg tgtccgaccc cgaggcgagc gggccgctcg
     1261 gcatcatcga ggcggaaggc gatttcggcc gcttccgctt cgagatcctg gcctatgcgt
     1321 cgcgcagcaa cccgaagacc tcggcgctca ccgcccacag cgtggtcgcc gcgctgcgcg
     1381 agggcatggc attcgaggtg gatcgtcttg acgccgaaaa aaataagggg gtacactgat
     1441 taaataagcg cggtaccctg cgcaacgagg agcgtcgtgg gctggcacga aatccccgag
     1501 gcagcgtcgc tgcccgacga ggaggcgagg gcggtggtga tcggacagcg ccagatcgcg
     1561 ctctgccgca gcggcggcac cctgcacgcg atcgacaacg tctgcacgca ccagtacgcg
     1621 ctgctctcgg agggctacgt cgaggacggg tgcatcgagt gcccgctgca ccaggggcgc
     1681 ttcgacctga agaccggcac cccgcagggc gccccggtca ccgagccggt gcgcgtctac
     1741 ccgctgcgca ccgagggcgg caagacgtac gtagagcttc cggactgacc gccatgccgc
     1801 tcgaacgcat cgtgatcatc ggcgccgggc aggcgggcgg gcgtgccgcc gaggcgctgc
     1861 gcagcgccgg cttcaagggc gaggtcctgc ttctcgggga cgaagcgcac gagccctacg
     1921 agcgcccggc gctctccaag ggcgtgctgc tgggcgagga gacggccgcg cgcacgctca
     1981 tccatccgcg cgagtggtac gcggcgcaga agatcgatct tcgcaccgcc acgcgcgtcg
     2041 aggcgatcgc gccggtcagc cagacggtga tcacggcgca aggcgagcgc atcgagtatt
     2101 cgcgcctgct cctgtgcacc ggcagccgcg tgcggcccct cgccggagcc aacgaaagcc
     2161 tgcgcggcgt gcactacctt cgcacgatcg gcgacagcga gcggctcgca gccgagctcc
     2221 atcccggccg ccgcatcgtc atcatcggcg gcggattcat cggcctcgag gtcgcatcga
     2281 gcgcggccaa gcgcggcctc gcggtgcgcc tggtggagcg gcaggccgca ttgctcgatc
     2341 gcgcgctgcc cgccgagatc gccgcgctcg tcgagtcgtt ccaccgcagc cagggcgtgc
     2401 agctgcacct tggcgtgccg gtcgcgcgca tccacggcag ggaagcagtc gagagcgtgg
     2461 agctcgccga cggcaccaac ctggatgccg acctggtcgt ggtcggcatc ggcatcatcc
     2521 ccaataccga gcttgccgaa gccgccggcg cggcctcgag cgacggcatc gtggtcgacg
     2581 aatattgccg cacgaacgtg gccaatctct acgccgcggg cgatgcgacg agccattaca
     2641 acccgcttct cgagcgccgg gtcaggctcg agtcgtggca gaacgcgcag aaccaggcgg
     2701 tggcggccgc ccataacctg agcggcaacg cccggcccta cgccgagatc ccgtggttct
     2761 ggtccgacca attcgggcgc aacatccaga tggtgggaat cgccgaaccc gggctcgagg
     2821 tgtcgtggcg cggtgagccg cgctccgggc gctgcatggc cttcgggctt cgcgacggcc
     2881 gggtgcg
//
LOCUS       DATDMP010000172         3125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1582542, whole genome shotgun sequence.
ACCESSION   DATDMP010000172 DATDMP010000000
VERSION     DATDMP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582542"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VI195_02875"
     CDS             <1..1523
                     /locus_tag="VI195_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6823358.1"
                     /translation="LFDEYLHRSLQFHLACNSATLFNHVVWAVTRGTTGMLQSLLLVV
                     TNVATSVLIIAAIVIVHPLVASVAAVALCASYGLVYLLARQRLLRNGMLESRHNEERA
                     KIASEAFGAIREIIVLHGQAFFRQKFAHSSAALSRAALNTNTIALSPRYLLEAIVAAA
                     LVVGATMSLGRDRSSGEWLAELSFLGFAAYRLLPSLQQIFHGVVKIRGDRAAFNRIAA
                     DLRAACRPAGRSVRTADALTWQGRPHAAITLRGVRFSYASDRPPAIRDVHLTIAAGAT
                     VALVGPSGSGKTTLAELILGLLSPTAGTIEVDGIALSASNRGDWQSAIAYVPQHAFLF
                     DASLAENVALATDFEHIDLERLSEALRLAQLEEFVHTLPRGYCEIVGEHGVRLSGGQR
                     QRVGIARALYRRASLLVLDEPTSALDGLTESEVMSAIEGLKGQCTIILIAHRTGTVRE
                     CDLIFELDAGRVVTTGTYAQLARQSERFERLLHGGEATAGRSVREPAGAGAGVHKE"
     gene            complement(1480..2214)
                     /locus_tag="VI195_02880"
     CDS             complement(1480..2214)
                     /locus_tag="VI195_02880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6823359.1"
                     /translation="MSVLPILCYHNVAPAPADTRFKLLYVSPESFERQLWSLRRLGLR
                     GVSMGEGIARLRNATARGCVAFTFDDGYADALATAAPLMKRYGFTATCYVVCDALGTY
                     NHWDAEYLGERKPLMSRTQLEQWLAAGMEVGSHSCSHPRLSQLAADALGHEIAESRAT
                     LRRMLGVPIEHFAYPFGHFTNETVEWVRRAGYSSAVTVLPGVARAADDPLRLPRILVN
                     GARGLWNYLLHVATPYERLRRRRQAP"
     gene            2263..2928
                     /locus_tag="VI195_02885"
     CDS             2263..2928
                     /locus_tag="VI195_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6823360.1"
                     /translation="MSIQSVGRHGLMDQAARWLQLLLDRGRRSDGLLQSAQIWEAQYA
                     SGRWDFLAELSELARFSVLAGYICHLKPGAAVLDTGCGQGLLLRRLPSALYSRYVGID
                     LAGSAIAVARQQQQDARSTFLEADCESYTPGEQFDVIVFNEVLCCLVDPLRTVERYVG
                     SLNPGGLLLVSMCTAARGSATILWRLKRRYATVDEVRVVHSARKVSWLCTAFSPGENG
                     AQP"
BASE COUNT          561 a          983 c         1032 g          549 t
ORIGIN      
        1 cactgttcga cgaatactta caccgcagcc tgcagttcca cctcgcctgc aatagcgcga
       61 cattattcaa tcacgtggtc tgggcggtaa cccgcggaac cacgggaatg ctgcagtctt
      121 tgctgctggt ggtgactaac gtagcgacca gcgtgctgat catcgctgcg atcgtcatcg
      181 ttcatccatt ggtcgcctcg gtcgctgcgg tcgcgctttg tgccagctac ggtctggttt
      241 acctgctcgc gcgccagcga ctgctgcgca acggcatgct cgagagtcgg cacaacgagg
      301 aacgcgcgaa gatcgccagc gaggccttcg gcgcaatacg cgagatcatc gtgctgcacg
      361 gtcaagcgtt tttccgccag aagttcgcgc acagctccgc cgcactctcc cgtgctgcgc
      421 tcaatacgaa cacgattgcg ctcagtccgc gctaccttct ggaagctatc gttgcggcgg
      481 cgctggtcgt tggtgcaacg atgtcgctcg gtcgggacag aagcagtggc gagtggctgg
      541 cggagctcag ctttcttggc ttcgcagcct accgcctgct gccctcgctg cagcagatat
      601 tccatggagt cgtgaaaatc cgcggcgatc gcgccgcgtt caaccgcatc gctgcggacc
      661 tgcgcgccgc ctgtcgcccg gcgggcagga gcgtacgcac tgcggatgcg ctcacctggc
      721 agggacggcc acacgcggca atcacgctgc gcggcgtgcg gtttagctac gccagcgacc
      781 ggccgccggc aattcgcgac gtgcatctga cgattgcagc gggcgcgacg gtcgcgttgg
      841 tcgggcccag cggctcgggc aagacgacac ttgccgagtt gatcctggga ctcctgtcgc
      901 ccaccgcggg gacgatcgag gtcgacggca tcgcgcttag cgcgagcaat cgtggcgact
      961 ggcaaagcgc catcgcctat gtgccgcagc acgcttttct gttcgatgcc tcgctggcgg
     1021 aaaacgtcgc gctggcgacc gacttcgagc acatagacct cgagcggctc agcgaggcgc
     1081 tgcgactcgc gcagctcgag gaattcgtgc acacactgcc gcgcggctat tgcgagatcg
     1141 ttggcgaaca tggcgtgcgc ctgagcggtg gtcagcgcca gcgggtcgga atcgctcgag
     1201 cgctctatcg gcgcgcgtcg ctgttggttc tcgacgagcc caccagtgcg ctcgatgggc
     1261 tcaccgagag cgaggtcatg tcggcgatcg agggcctgaa gggacaatgc accatcattc
     1321 tgatagcgca ccgcaccggc acggtgcgag agtgcgacct gatcttcgag ctcgacgctg
     1381 gcagggtggt caccaccggc acctacgcac agctcgcgag acaatccgag cggttcgaac
     1441 ggctcttgca cggaggcgaa gcaaccgccg ggcgatcggt cagggagcct gccggcgccg
     1501 gcgcaggcgt tcataaggag tagcaacgtg caacaggtaa ttccacaggc cgcgcgcgcc
     1561 attcaccagt atgcgcggca gccgcagtgg gtcgtccgcc gcccgggcca ctcccggcaa
     1621 gacggtgacc gccgaggagt agccggcgcg ccgcacccac tcgactgttt cgttggtaaa
     1681 gtgcccgaac gggtaagcga aatgctctat cggaacgcca agcatgcggc gcagcgtagc
     1741 gcgactctcc gcaatctcgt ggcccagcgc atccgccgcc aattgcgaca gccgcggatg
     1801 cgagcacgag tgcgagccta cttccatgcc cgcagccaac cactgctcga gctgcgtccg
     1861 gctcatgagc ggctttcttt ccccgagata ctcagcgtcc cagtggttgt aggttccgag
     1921 tgcgtcgcac acgacgtaac aggtggcggt gaatccatag cgcttcatga gcggcgccgc
     1981 cgtcgcgagt gcgtcggcgt acccgtcgtc gaaggtgaat gcgacacaac cacgcgccgt
     2041 ggcattgcgc agccgggcaa tgccttcgcc catggagacg ccgcgaaggc cgagacggcg
     2101 caggctccac agctgccgct cgaagctctc tggactcaca tacagcaact tgaagcgcgt
     2161 gtccgcgggg gcgggggcca cattgtgata acagaggatg ggaagcaccg acatgttgcc
     2221 tagaatatta ccggcgcggt cgctaagcat ggagctagcg ccgtgagcat tcaaagcgtg
     2281 ggtaggcatg gtcttatgga ccaggcggcg cgctggctgc agctgctgct ggaccgcggc
     2341 cgccgctccg atggcttgtt gcaatccgcc cagatctggg aagcgcaata tgcgtcaggg
     2401 cggtgggact tccttgcaga gctttccgaa ctcgcacgct tcagcgtgct ggcgggctac
     2461 atctgccacc tgaagccggg cgcagcggtg ttggataccg gatgtggcca ggggctgctg
     2521 ctgcgtagac tcccgagtgc cctatactcg aggtacgtag gcatcgacct ggccggcagc
     2581 gcgatcgccg tcgcacggca gcagcagcag gacgcacgca gtaccttcct cgaggcggac
     2641 tgtgagagct acacaccggg cgagcagttc gatgtcatcg tcttcaacga ggtgctctgt
     2701 tgcctggtcg atccgctgcg caccgtagag cgctatgtgg gttccctgaa ccctggtggg
     2761 ctgctattgg tgtccatgtg cacggccgcg cgcggcagtg ccacgatact gtggcgactc
     2821 aaacgcaggt acgcgaccgt cgatgaggtg cgcgtagtcc acagtgccag gaaagtctcg
     2881 tggctgtgca cggcgttcag cccaggggag aatggggcac agccctgagt ggggagggga
     2941 gatgcggggt ttgaaccgaa tggtgcgccc ggagccactt aaaaagatat ccccaaggtg
     3001 gaggccggct tgtacgcgaa ggccttgacg agcccgcggc acacggggca ggcgcgattc
     3061 ctcgaccatg catacgcagg gtaaagcatg atgccctcgg cagcgcccgc gccggtcgct
     3121 gcggc
//
LOCUS       DATDMP010000173         3081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1026901, whole genome shotgun sequence.
ACCESSION   DATDMP010000173 DATDMP010000000
VERSION     DATDMP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026901"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1092)
                     /locus_tag="VI195_02890"
     CDS             complement(280..1092)
                     /locus_tag="VI195_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6823361.1"
                     /translation="MTAISAYPHRIVCLTEETTETLYLLGEDWRIVGISGFTVRPPRA
                     RREKPRVSAFTSAHTARILALTPDLVLGFSDLQADIAAELVRAGLAVHVFNQRSVAGI
                     LAMIRTLGGMIGCQARAEALAARLEAGLGEVRARAARLPRRPRVYFEEWDEPQISAIG
                     WVSELITLAGGEECFPELARGALGRERIVADPLAVPRRAPDIVLGSWCGKKFRPERVA
                     ARPGWSEVPAVRAGYVREIKSAIILQPGPAALTDGVRAIQRVIEEWAKACSR"
     gene            1133..2482
                     /locus_tag="VI195_02895"
     CDS             1133..2482
                     /locus_tag="VI195_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6823362.1"
                     /translation="MSARIWAFLDWCFFGPASQRTGLAGRALRLLRYSYAVVRDLARG
                     DINLRAMSLVYTTLLSLIPLLAFSFAVLKLFGGHRDLVPVVYEFFRPVGEPAATELTT
                     RVMLFASHVSSGVVGSVGLALLAWTLLDTIRKVEDSFNFLWHVDQPRGFARRLAEYTT
                     LLIAGPVVLVGFIGLSHHALASAPVQEVARLEWLRSGAITLAPYAMVSAFFTALYMMV
                     PNTRVQWRAALTGAVVGGVLWAAIGKMFTALVVYSTRLTIVYAGFAFVVAALLWTYFG
                     WLILLAGAQLSFYVQNPTYLRLGLQQLRLSSVELEELALKLMYFVGRSHVTGGQRWTV
                     NGLATELGLPGIAVAQMATTLEHAGLVIVTDDDELVPARDIGHIGVHELLQIARDQKS
                     GHVAPRNVPIPPVDRVLSSVAEALRRSSGELTLRDLVEEVPRPTLALARSKGQSEGR"
     gene            complement(2507..>3081)
                     /locus_tag="VI195_02900"
     CDS             complement(2507..>3081)
                     /locus_tag="VI195_02900"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY6823363.1"
                     /translation="AIRGASQYRKASFLLGAAGRQVFPAFLQMHERPHLPKGLASAPF
                     DQEGAATHDRELVRDGVLGGYVLGSYSARRLGLKTTGNAGGIHNLIVASSAGALAPGE
                     FLARLGTGLLVTELMGQGVNGVTGDYSRGASGFWVENGQLVHPVHEVTIAGNLPGMYR
                     GIVALGADTDRRGAICTGSLLVGEMTIAGE"
BASE COUNT          421 a         1105 c         1093 g          462 t
ORIGIN      
        1 cgcgggcgcg agcgaccagg aggtggtcaa ggcctaccgc cgccagctcc accggcatca
       61 tcccgacaag ctcaaggcga acgggctgcc cgagtcgatg ctggcgcacg ccaagcagcg
      121 cacccagcag atcatcgaag cctacgagct catccgcgcg cgccgcggga tgtcgtagcg
      181 ttatcaactg gcaaaagtcg cctgcggcgc ttttgcggac agtgccggcg tttccggccg
      241 cgaaagacgc gactttcgcg gcctgctccg gaggcaacgc tagcgtgaac aggccttggc
      301 ccactcctcg atcacgcgct ggatggcgcg cactccgtcg gtgagcgccg ccggcccggg
      361 ttgcaggatg atcgccgact tgatctcgcg cacgtagccc gcgcgcaccg ccggcacctc
      421 gctccatccg gggcgcgccg ccacccgttc gggacggaat ttcttgccgc accaggagcc
      481 gagcacgatg tcgggggcgc ggcgcggcac cgcgagcgga tcggcgacga tgcgttcgcg
      541 cccgagggcc ccgcgcgcga gctcgggaaa gcattcctcg ccgccggcga gcgtgatcag
      601 ctccgacacc cagccgatgg cgctgatctg cggctcgtcc cactcctcga agtacacccg
      661 cgggcgcctg gggagccttg ctgcccgcgc acgcacctcc ccgagccccg cctcgagccg
      721 cgcggcgagc gcctcggccc gcgcctggca gccgatcatg ccaccgaggg tgcggatcat
      781 ggcgaggatg cccgcgacgc tgcgctggtt gaagacgtgc accgcgagtc cggcgcgcac
      841 cagctcggcg gcgatatcgg cctgcagatc cgagaagccg agcaccaggt cgggtgtcag
      901 ggcgaggatg cgcgcggtgt gggcgctggt gaaagccgat acccgcggct tctcgcgccg
      961 ggcgcgcggc gggcgcaccg tgaagccgga gatgcccacg atccgccagt cctcgcccag
     1021 caggtagagc gtctcggtcg tctcctcggt gaggcacacg atccggtgcg ggtaggctga
     1081 aatcgcggtc atgttggcgc ccgtccgggg gcgggcactg tatccttaga ggatgtcggc
     1141 gcgcatctgg gcgttcctcg actggtgctt cttcggcccg gcctcccagc gcaccgggct
     1201 cgcggggcgg gcgctgcgcc tgctgcgcta cagctacgcg gtggtgcgcg acctggcgcg
     1261 cggcgacatc aacctgcgcg ccatgagcct ggtgtacacg acgctcctgt cgctgatacc
     1321 cctgctcgcc ttcagcttcg cggtgctgaa gctcttcggc gggcaccgcg acctggtgcc
     1381 ggtggtgtac gagttcttcc gtccggtggg cgagccggcc gcgaccgagc tcaccacgcg
     1441 cgtcatgctg ttcgccagcc acgtgagcag cggcgtggtc ggctcggtgg gcctcgcgct
     1501 gctcgcctgg acgctgctcg acaccatcag gaaggtcgag gacagcttca acttcctgtg
     1561 gcacgtcgac cagccccgcg gcttcgcgcg gcggctggcc gagtacacca cgctgctcat
     1621 cgccgggccg gtggtgctgg tcgggttcat cgggctctcc caccacgcgc tcgccagcgc
     1681 tccggtgcag gaggtggcgc ggctcgagtg gctgcgcagc ggcgccatca cgctggcccc
     1741 ctacgccatg gtgagcgcct tcttcaccgc gctctacatg atggtgccga acacgcgggt
     1801 gcagtggcgg gcggcgctca ccggcgccgt ggtgggcggg gtgctgtggg ccgcgatcgg
     1861 caagatgttc accgcgctgg tggtgtactc gacgcgcctc accatcgtgt acgcgggctt
     1921 cgccttcgtg gtggcggcgc tgctgtggac ctacttcggc tggctgatcc tgctcgccgg
     1981 ggcgcagctg tccttctacg tgcagaaccc gacctacctg cgtctcggtc tgcagcagct
     2041 gcggctctcg agcgtcgagc tcgaggagct ggccctcaag ctcatgtact tcgtcggccg
     2101 cagccacgtg accggcgggc agcgctggac ggtgaacgga ctcgccaccg agctcgggct
     2161 gcccggcatc gcggtggcgc agatggccac gacgctcgag cacgccgggc tggtgatcgt
     2221 gaccgatgac gatgagctgg tgcccgcgcg cgacatcggg cacatcggcg tgcacgagtt
     2281 gctgcagatc gcgcgcgacc agaagagcgg ccacgtcgcg ccgcgcaacg tgccgatccc
     2341 gccggtggac cgcgtgctct cgagcgtagc cgaagcactg cgccgcagca gcggcgagct
     2401 cacgctgcgc gacctggtcg aggaggtgcc gcggccgacg ctggcgctgg cccgcagcaa
     2461 gggacagtcg gaagggcgct aggccccgcc cgtgcgcgcc cgctggtcac tcaccggcga
     2521 tggtcatttc cccgacgagc agcgagccgg tgcagatcgc gccgcgccgg tcggtgtcgg
     2581 cgccgagggc gacgatcccg cggtacatgc ccgggaggtt gccggcgatc gtaacctcgt
     2641 gcaccgggtg gacgagctgc ccgttctcga cccagaagcc gctcgccccg cgcgagtaat
     2701 cgccggtcac gccgttgacg ccctgcccca tgagctcggt gaccagcagc ccggtgccga
     2761 gacgcgccag gaactccccg ggtgcgagcg ccccggcgct gctcgcgacg atgaggttgt
     2821 ggatcccgcc ggcgttgccg gtggtcttca gtcccagccg ccgcgccgag tagctgccga
     2881 gcacgtaccc cccgagcacc ccgtcgcgca ccagctcgcg atcgtgcgtg gcggcgccct
     2941 cctggtcgaa gggcgcgctc gccagcccct tcggcaggtg cggccgctcg tgcatctgca
     3001 ggaacgccgg gaacacctgc cggccggccg cgccgagcag gaacgaggcc ttgcggtact
     3061 ggctggcgcc gcggatcgca c
//
LOCUS       DATDMP010000174         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1915877, whole genome shotgun sequence.
ACCESSION   DATDMP010000174 DATDMP010000000
VERSION     DATDMP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915877"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VI195_02905"
     CDS             complement(<1..830)
                     /locus_tag="VI195_02905"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY6823364.1"
                     /translation="MARDEFARRRRQLMRLMGRDSIAVLPAAPVRHRNGDVEYAFRQD
                     SDFHYLTGFGEPEAVAVLVPGRTQAEYILFVRERDPARETWDGRRAGPAGAARDYGAD
                     DAFPVTDIDDILPGLLENRTRVYYTMGEYADFDQRVLGWVNGLRTQAKNGRHPPQEFV
                     ALDHVLHDMRLYKSRGELELLRRAAQIATQAHLRAMRFCRPGASEYQVMAELLHEFRR
                     HNADTAYQPIVGGGANSCILHYRENDQPLSSGDLLLIDAGCEFECYASDVTRTFPVNG
                     R"
     gene            complement(849..1403)
                     /locus_tag="VI195_02910"
     CDS             complement(849..1403)
                     /locus_tag="VI195_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEY6823365.1"
                     /translation="MIAPDYNHIEQLLRQERSLADAAEAHGTLSGCLCATTGYRLEDW
                     LHEILPEGRAAPESSAALGELFTATAAALIQPDMELELLLPADEQPIEMRTAALAHWC
                     QGFLYGLGAGAITDATGLPGDAGEIVRDFTEISRAAVDATQEEESNEAAYAELVEFVR
                     VGVQLLFEELAALRRPAPAAAPLH"
     gene            1475..1705
                     /locus_tag="VI195_02915"
     CDS             1475..1705
                     /locus_tag="VI195_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HEY6823366.1"
                     /translation="MNDTTKTQLDLELTRLGKRIDELLAVVEHLREENRALRARHESL
                     AGERAALMHKNEQVRTRVEAMIGRLKTLEHGA"
     gene            1702..2019
                     /locus_tag="VI195_02920"
     CDS             1702..2019
                     /locus_tag="VI195_02920"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY6823367.1"
                     /translation="MTQPDQARVSVRIMEKEYVIACPYDDRSALLDAAEFLNARMREI
                     RDSGRVVGIDRIAVMAALNLAHEYLKGKDRESKLDNGVGQRVRALRERVEGALGKSQQ
                     LEL"
     gene            2068..2257
                     /gene="ssrS"
                     /locus_tag="VI195_02925"
     ncRNA           2068..2257
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VI195_02925"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          414 a          805 c          768 g          380 t
ORIGIN      
        1 cgaccgttca ccgggaaggt gcgggtgacg tccgaggcat aacactcgaa ctcgcagccc
       61 gcatcgatca gcagcaggtc gccgctcgag agcggctgat cgttctcgcg gtaatgcagg
      121 atgcagctgt tggcgccgcc ccccacgatc ggctgataag cggtgtcggc gttgtgccgc
      181 cggaactcgt gcagcaactc cgccatgacc tggtactcgc tggctccagg tcgacagaaa
      241 cgcatggcgc gcaggtgcgc ctgcgtggcg atctgcgccg cgcggcgcag cagctcgagc
      301 tcgccgcggg acttgtacag ccgcatgtcg tgcagcacgt gatcgagcgc cacgaactcc
      361 tgcggcggat ggcggccatt ctttgcctgg gtgcgcagcc cgttcaccca gccgagcacg
      421 cgctgatcga agtcggcgta ttcccccatc gtgtagtaga cccgggtgcg gttctcgagc
      481 agccccggga ggatgtcgtc gatgtcggtc accggaaagg catcgtccgc gccgtaatcg
      541 cgagctgccc cggcgggacc tgcacgccgg ccgtcccagg tctcgcgggc cgggtcgcgc
      601 tcgcgtacga acaggatgta ttccgcctgc gtacggccgg gcacgagcac cgccaccgcc
      661 tcaggctcac cgaagccggt gagataatga aagtcactgt cctgacggaa ggcgtactcg
      721 acatcaccgt tgcgatgcct gacgggcgcc gccggcagga ccgcgatcga gtcgcgcccc
      781 atgaggcgca tcagctggcg acgtcggcgc gcgaattcat ccctcgccat gggcggtgcg
      841 gttcctcctc agtgcagcgg cgccgccgcc ggcgccggcc ggcgcagcgc ggcaagctct
      901 tcgaacagca gctgcacccc cacgcgtacg aactcgacca gctccgcgta cgccgcctcg
      961 ttcgattcct cctcctgcgt ggcgtcgaca gcggcgcggc tgatctcggt gaaatcgcgc
     1021 acgatctcgc ccgcatcccc cggcaacccg gtcgcgtcgg tgatcgcacc ggcaccgagt
     1081 ccgtaaagga agccctggca ccagtgggcc agcgccgcgg tacgcatctc gatgggctgc
     1141 tcatcggcag gcagcagcag ctcgagctcc atgtcaggct gtatgagtgc ggccgccgtc
     1201 gcggtgaaca actccccgag cgccgcggag gattccggcg ccgcgcgccc ctcgggcagg
     1261 atctcgtgca accaatcctc gagccgatat cccgtcgtgg cgcacagaca cccggacagc
     1321 gtgccgtgtg cctcggcggc atcggcgagt gagcgctcct gccgcagcag ctgctcgata
     1381 tggttatagt ccggcgcgat catggtgcac gggcacatta tataatcgct acccagaatt
     1441 gaccgcgtcg agaacgcggc tctatagtct tgccatgaac gacaccacaa aaacacaact
     1501 ggatctcgag ctcacgcgtc tcggcaagcg catcgacgag ctgctcgccg tggtggaaca
     1561 cctgcgcgaa gagaaccgcg ctctgcgtgc ccgtcacgag tcactcgcgg gcgagcgggc
     1621 cgcgctcatg cacaagaacg agcaggtgcg cacgcgcgtc gaggccatga tcggacggct
     1681 aaagactctg gagcacgggg catgacgcag cccgaccagg cccgggtgag cgtacgcatc
     1741 atggagaagg aatatgtcat cgcctgcccc tacgatgacc gttccgccct gctcgacgcc
     1801 gccgagtttc tcaatgcgcg catgcgcgag atccgcgaca gcggccgggt cgtcggcatc
     1861 gatcgtatcg ccgtgatggc cgcgctcaac ctcgcgcacg aatacctcaa gggtaaggac
     1921 cgcgagtcga agctcgataa cggcgtcggc cagcgggtgc gtgcgctgcg tgagcgcgtc
     1981 gagggtgcgc tgggaaagag ccagcagctc gagctgtgac gcgctcgagc gcctaagggc
     2041 cgaacaattg gtgtagagtg cttctggcgt cgcctgcggt gttcgacaag cgggccgggt
     2101 tacctcggac cctaattgaa acaccctcgg gacttcaggc ttgcgcgcag cgctgtgcaa
     2161 gtccgccctc gagcggaaag ccttacctgc cgcagttcgt cccacttgtt gctctggttc
     2221 gagagcaacg gttcgcaccg gcacatgcgg gtggcgctat ctgattctcc cacagcaggt
     2281 gccgcccgca gatcactgcg ggcggacctc aacgcacggc gccgccaggt atcgcgccag
     2341 cagcgtgcgc aagcctcaca gctcgtc
//
LOCUS       DATDMP010000175         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_471719, whole genome shotgun sequence.
ACCESSION   DATDMP010000175 DATDMP010000000
VERSION     DATDMP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471719"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VI195_02930"
     CDS             complement(<1..993)
                     /locus_tag="VI195_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6823368.1"
                     /translation="MSSSMQGKLLSPVLMLGAAAETRGSIAAVVDAYRVHGFFRRWPV
                     EYLETHSSGGLLQDARQTLKAVAHFASRLARERRAAVHVHSTARTFWRDTPFIAAALL
                     ARCPVILQLHGGGFERLHDAASTPARAALHAILERASGIVVPAESLRGWVRRMHRNAP
                     VYCIPSPVNVPQVRRDAPRGNVVLFLGRLDAAKGIFDLLEAVAALRGSIPDLRLVCAG
                     EGDRAAVARYAERLGIRDSVKFTGWVGPSGKRALLESAAVFALPCYSAGMPMSLLEAM
                     SAGIPAIAAPVGGIPEVLTDGVSGMLVAPGDTATLSRNLRKLLVEPDFAQRIGAA"
     gene            complement(995..>2617)
                     /locus_tag="VI195_02935"
     CDS             complement(995..>2617)
                     /locus_tag="VI195_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823369.1"
                     /translation="AVPERRNPGMAALSALAPATSPQARKLRVGLFADTRLQPQWVVE
                     SFARIARSECAEIVAICCGDAPPPELPWTWKLYDRLDRAAFRAGRDPLALADLTRLPH
                     HKHLHSGSEADFLALDLDVAFALGRFDDKALEGIAHYGVWRYCFGEQRHEEEALAGVQ
                     EVVRGLPVSGSGVKVRIGGSVRLAYQSWSRTYPFSVGRNRAQLLRKTAEFAQRALAEL
                     HRSGRDWLEQCKLVVPAPAVAASRERKLAPILGRIAERGLQKALGLEQWFLAYRIRES
                     RFGDARAIAPDLAGFTRLMPPRDRYWADPFVLEKGGRYFVFFEELMFRTGRAHISMLE
                     IDAAGRASAPIKVLQRDYHLSYPFVLEHDGSLYMIPETAQNGTIEAYRCVDFPHSWRL
                     ERILVSGVHCVDATFHQGSGRWWMFGNVAAAGATMFDDELHVFHADRLLGEWQPHARN
                     PVKSDTRSARPAGKLYWHNGALYRPAQICAPLYGSGLSINRVMRLTTQDYAERQVERI
                     LPAEKAGLLGVHTLNRAGDLTIVDAFTRRRRF"
BASE COUNT          440 a          896 c          916 g          365 t
ORIGIN      
        1 cgcggcgccg atgcgctgcg caaaatccgg ctcgaccagg agcttgcgca ggtttcgcga
       61 cagggtcgcg gtatcgcccg gcgcgacgag catcccgctt acgccgtccg tgagcacttc
      121 agggatgccg ccgaccggcg cggcgatcgc gggaatgcct gccgacatgg cctcgagcag
      181 gctcatcggc atgcctgcgc tatagcaggg caacgcgaaa acggcggcgc tctcgaggag
      241 cgcgcgcttc ccggaggggc cgacccagcc ggtgaatttc accgagtcgc ggatgccgag
      301 gcgttccgcg tagcgcgcca ccgccgcgcg atcgccctcg cccgcgcaca ccaggcgcag
      361 gtccggaatc gatccgcgca gcgctgcaac ggcttccagg agatcgaaga ttcccttggc
      421 tgcgtccagg cgcccgagga agagcaccac gttgccgcga ggagcgtcgc gccgaacctg
      481 cggaacgttc accggactcg ggatgcaata caccggcgcg ttgcggtgca tgcgccgcac
      541 ccaaccgcgc agggactcgg caggcaccac gatccccgac gcgcgctcga ggatggcgtg
      601 caacgccgcg cgagcaggcg tgctcgcggc gtcgtgcagg cgctcgaagc cgccgccgtg
      661 cagttggaga atcaccgggc agcgcgcgag cagcgcggct gcgatgaaag gcgtgtcgcg
      721 ccaaaacgtg cgtgccgtgc tgtgcacgtg caccgccgcg cgcctttcgc gcgcgagccg
      781 ggaggcgaaa tgcgccaccg ccttcagggt ctggcgcgcg tcctggagca ggccgccgct
      841 cgaatgcgtc tcgagatatt ccacgggcca gcgcctgaag aagccgtgca cgcggtaggc
      901 gtcaacgacg gcggcgatgc tgccgcgcgt ctccgcggcg gcgcccagca tgaggacggg
      961 ggaaagaagc ttgccttgca tggaggagct catgtcagaa acgacggcgg cgcgtgaaag
     1021 cgtcgacgat ggtgagatcg cccgcccgat tcagcgtgtg cacgcccagc aggccggctt
     1081 tctcggccgg gaggatgcgc tccacctggc gctccgcgta atcctgcgtg gtgagccgca
     1141 tcacccggtt gatcgaaagg cccgagccgt agagcggcgc gcagatctgc gccggccggt
     1201 acagggcgcc gttgtgccag tagagcttgc ccgcgggccg cgcgctgcgc gtatcggact
     1261 tcaccgggtt gcgcgcatgc ggctgccatt cgccgagcag ccggtcggca tggaagacat
     1321 gcagctcgtc gtcgaacatc gtcgcgcccg ccgcggccac attgccgaac atccaccagc
     1381 ggcccgatcc ctggtggaag gtggcgtcca cgcaatgcac gcccgagacg aggatccgct
     1441 ccaggcgcca cgagtgcggg aaatctacgc aacggtaggc ttcgatggtg ccgttctgcg
     1501 ccgtctcggg aatcatgtag aggctgccgt cgtgctcgag cacgaacggg tacgaaaggt
     1561 gatagtcccg ctgcagcacc ttgatcggcg ccgacgcgcg gccggcggca tcgatctcca
     1621 gcatcgagat gtgcgccctg ccggtgcgga acatcagctc ctcgaagaac acgaaatagc
     1681 ggccgccttt ctcgagcacg aagggatcgg cccagtagcg gtctctgggc ggcatcagcc
     1741 gggtgaatcc cgcgaggtcg ggcgcgatcg cgcgcgcgtc gccgaagcgg ctctcgcgga
     1801 ttcgataggc gaggaaccat tgctcgagcc cgagcgcctt ttgcaggccg cgctccgcga
     1861 ttcgcccgag gatcggcgcg agcttgcgct cccgggatgc ggcaacggcc ggcgcgggaa
     1921 cgaccagctt gcactgctcc agccagtcgc gccccgagcg gtgaagctcg gcgagcgcgc
     1981 gctgggcaaa ttccgccgtc ttcctcagga gctgcgcccg gttgcgcccg acggagaaag
     2041 gataagtgcg cgaccaggac tggtaggcga ggcgaacgct gccgccgatg cgcaccttca
     2101 cgcccgagcc gctgaccggc aatccgcgca cgacttcctg cacgcccgcc agcgcctcct
     2161 cttcatggcg ctgctcgccg aagcaatagc gccagacacc gtagtgcgcg atgccctcca
     2221 gggccttatc gtcgaaacga ccgagggcaa acgcgacgtc gagatcgagc gcgaggaaat
     2281 cggcttccga gcccgaatgc agatgcttgt gatgcggcag gcgcgtgagg tcggcgagcg
     2341 cgagcggatc gcgccccgcc ctgaaggcgg cgcggtccag ccggtcgtag agcttccagg
     2401 tccacggcag ctcgggcggc ggcgcatcgc cgcagcaaat cgccacgatc tcggcgcatt
     2461 cggagcgtgc aatgcgcgcg aagctctcca ccacccactg cggctgcagg cgcgtgtccg
     2521 cgaacaggcc cacgcggagc ttgcgcgcct gcgggctggt ggcgggggcg agcgcgctca
     2581 gagccgccat acccggattc ctgcgctctg gaacggc
//
LOCUS       DATDMP010000176         3298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1915964, whole genome shotgun sequence.
ACCESSION   DATDMP010000176 DATDMP010000000
VERSION     DATDMP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915964"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1015)
                     /locus_tag="VI195_02940"
                     /pseudo
     CDS             complement(1..1015)
                     /locus_tag="VI195_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
     gene            complement(936..2606)
                     /locus_tag="VI195_02945"
     CDS             complement(936..2606)
                     /locus_tag="VI195_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY6823370.1"
                     /translation="MGRFAVFAVAFAVAGSALAQSPPLADAVEKAAHSTITRAALEGP
                     IRFLSSDLLEGRGPGTRGDQLARLYLQTRLEGMGYQAAFANGAWQQPFDIVGIKGQFP
                     KSWSFQGRGQRVDLAWHEDYIAVSGLQNENVAIDDAELVFVGYGIQAPEFHWDDFKGM
                     NLTGKILVMMNNDPDWDPQLFAGKRRLYYGRWVYKYESAARRGAAGAIIIHTTPSAGY
                     PWQVVQSSWGGEQFALPAGDEPHVRVEAWATEAAVRRLLQSSGYDLDKLVAAAHARDF
                     HPVPLGIRTAIKFTNTLSRVQTANVGGVLPGSDARLAPQLVVLSAHHDHFGIGEPDAS
                     GDRIYHGAVDNASGCAQVLAIAQAFAALPERPRRSMLALFVAGEERGLLGSKYYAEHP
                     TVPAGRIAADINIDGGNIFGRTRDATSIALGKSSLDAIAAQVAATQGRVVKGDQFPDR
                     GYYYRSDQFSFARIGVPALFFDDGTDFIGRPPSWGREQIEQWEMKKYHQPSDRLEDSW
                     NFDGMIEDAQLAMLSAWRVAQADAMPSWKPGDEFEAARKRALAAVGGP"
     gene            2732..>3298
                     /locus_tag="VI195_02950"
     CDS             2732..>3298
                     /locus_tag="VI195_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEY6823371.1"
                     /translation="MAHSSAILQLRILICIAGSALPAVASASDASPPPPANRAAVQEL
                     PTVTVIANTPLSGLGLPLNQIPANVQTGDSADMRRQLSLDLADYLNNNFSGVNVSESA
                     ANPFQLDVNYHGFTASPLLGTPEGLSVYVDGVRVNESFGDTVNWDLIPQAAISTLTLM
                     SGSNPVFGLNTLGGALSVRTKSGPDNPGT"
BASE COUNT          543 a         1142 c         1082 g          531 t
ORIGIN      
        1 tcaggacccc gtggacgatg gcggcgccgt gatccggcgg catcgaccag agaccgcgag
       61 cgaggcgcac cagctggctc atggcggcat ccgcctcgcc cggcgatgcg cacaggatgc
      121 cgagcgcgcc ggcgcgctcg cggtagagcc cgaagttctt cgagcacgac acggcgaaca
      181 tcatctcggg gagctcggcg cagaacagcc gcagcccgaa ggcatcggca tcgagccctt
      241 cgccgaggcc ctggtacgcc atgtcgatga acggcagcag ctgccggcgc ttcaccagcg
      301 acaggagctc acgccactcg ctctgcgaca ggtcggcgcc ggtcgggttg tggcaggagg
      361 cgtgcagcag cacgacggcg cgcggcggca gccgctcgaa cgccgccagc atcccggaga
      421 acgacacccc gccgcgcgcg gcatcgtatt acgggtagcg ctcgagcttc agtccacttc
      481 ccgagagcag cggcacatga ttgacccagg tcggggtact cacgtgcacc accgaatcgg
      541 gcgaggcgtg gcggatgagc tccgctccga gccgcagcgc gccgcagccg cccggtgtct
      601 gcacgctgcg cacccgtccg cccgcgagtg ccggatgtgc ggcgccgagc accagttgct
      661 ccatggactg gttgaaagcg ggattgcccg ccggcccgat gtaggtcttg gtggtctggc
      721 gcgccagcag cgcgccctcg gcggcgcgca ccgactccag caccggcgtg ttgccctgct
      781 catcgcggta caccccgatg ccgaggtcga ccttgcgcgg atccgggtcg gcccggaacg
      841 ccgccatcag gccaaggatg gggtcgggga caaccgcgtc gaaatgctcg aacatgcttt
      901 tctcctggag acgagccgct gcgtgcgcgc ctccgtcagg gtccgcccac cgcggcgagt
      961 gcgcgcttgc gcgccgcttc gaactcatcc ccaggcttcc agctgggcat ggcatccgcc
     1021 tgcgcgacgc gccacgcgga cagcatcgcg agctgtgcgt cctcgatcat gccgtcgaag
     1081 ttccagctgt cctcgaggcg gtcggagggc tgatgatatt tcttcatctc ccactgctcg
     1141 atctgctcgc ggccccagct cggcggccgg ccgatgaaat cggtgccgtc atcgaagaac
     1201 aacgccggca cgccgatgcg cgcgaaggag aactggtcgg agcgatagta gtaaccgcgg
     1261 tcggggaact ggtcaccctt cactacgcgg ccctgagtgg ccgcgacctg cgccgcgatc
     1321 gcatcgagcg aggacttgcc gagcgcgatc gaggtggcat cgcgcgtacg gccgaagatg
     1381 ttaccgccgt cgatgttgat atcggcagcg atgcggcccg cggggacagt cggatgctcg
     1441 gcgtagtact tcgagccgag caggccgcgc tcctctcccg caacgaacag cgccagcatc
     1501 gagcgccgcg gccgttccgg cagcgccgcg aacgcttgcg cgatcgccag cacctgcgcg
     1561 cagccggaag cattgtcgac cgcgccgtgg tagatgcggt cgccgctcgc gtcgggttcg
     1621 ccgatgccga agtggtcatg gtgcgccgag agcaccacga gctggggggc gagtcgcgca
     1681 tcactgcccg gcagcactcc gccgacattg gcggtctgca cccgcgagag ggtgttggtg
     1741 aatttgatcg cggtgcgtat gccgagtggc accggatgga agtcgcgcgc gtgcgccgcg
     1801 gccacgagct tgtccagatc gtaaccgctc gactgcagca gccgccgcac cgcagcctcc
     1861 gtggcccagg cttccacacg cacgtgcggc tcatcgcccg ccggcagcgc gaactgctcg
     1921 ccgccccagg agctctgcac cacttgccac ggatagccgg cggatggggt cgtatggatg
     1981 atgatggcgc cggctgctcc ccggcgcgcg gcgctttcgt atttgtatac ccagcgcccg
     2041 tagtacagcc ggcgcttgcc ggcgaacagc tgcggatccc agtcgggatc gttgttcatc
     2101 atcaccagga tcttcccggt gaggttcatg cccttgaagt cgtcccagtg aaactccggc
     2161 gcctggatgc cgtaaccgac gaacaccagc tcggcatcgt cgatggcgac attctcgttc
     2221 tgcagaccgc tgacggcgat gtagtcctcg tgccaggcga gatccacgcg ctggccgcga
     2281 ccctggaagc tccagctctt cgggaactgc cccttgatgc cgacgatgtc gaacggctgt
     2341 tgccaggcgc cattggcgaa tgccgcctgg tatcccatgc cttcgagtcg cgtctgcaga
     2401 tagagtcgtg ccagctgatc gccgcgcgtg cccgggccgc gcccctcgag cagatccgat
     2461 gacagaaagc ggatcggccc ctcgagagcc gcgcgtgtga tggtgctgtg cgccgctttc
     2521 tcgaccgcat cggcaagcgg cgggctttgc gccagcgcgc tgcccgcgac cgcaaacgct
     2581 acggcgaata cggcgaatct gcccataccc gaaactgcga tcacgtgctg ctctccgcgc
     2641 cggggagggc gcaacggtag cacgcagctg cgcccggaat catctggacg gatgcagcac
     2701 gcggtctcta cactcgatcc acggaaggct gatggcccat tcgagtgcga ttctgcagct
     2761 gcggatcctg atttgcatcg ctggctcagc gctgcctgcc gtcgcgtctg cgagtgatgc
     2821 ctcgcccccg ccgccggcga atcgggccgc ggtgcaggag ctgccgacag tcacggtaat
     2881 cgccaacacc ccgctgtcag gtctcggact gccgctcaat cagataccgg ccaacgtgca
     2941 gaccggcgac agcgcggaca tgcgccggca gctgtcgctc gatctcgccg attacctgaa
     3001 caacaacttc agcggtgtca acgtcagcga gagcgccgcc aatccctttc agctggacgt
     3061 caattatcac ggcttcaccg cttcgccgct gctcggtacc cccgagggct tgtcggtgta
     3121 cgtcgatgga gtgcgcgtca acgagtcatt cggcgatacc gtcaactggg atctgatccc
     3181 gcaggcggcc atctcgacgc tgacgctgat gtcgggctcc aacccggtgt tcggactcaa
     3241 cacgctcggc ggcgcgctct cggtgcgcac caagagcgga cccgacaatc ccggaacc
//
LOCUS       DATDMP010000177         4058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1582841, whole genome shotgun sequence.
ACCESSION   DATDMP010000177 DATDMP010000000
VERSION     DATDMP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582841"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /gene="asnB"
                     /locus_tag="VI195_02955"
     CDS             complement(<1..1236)
                     /gene="asnB"
                     /locus_tag="VI195_02955"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6823372.1"
                     /translation="MCGIAGIVGPSNELGEGMQRMLAVLRHRGPDGVGVEQDAHAILG
                     HRRLSIIDLAGGRQPLSNADKSIWLVCNGEIYNYRALRANLENRGQRFLTQSDCEVII
                     GLYELYGDRLLEHLRGMFAFALWDSRRRRLLLARDHLGQKPLYYAHSARGFAFASEIK
                     ALLAFDDTLRTLDLAALDQYLALRLIAPPLSMFRGIRKLPPAHLLVLEPEREPVIRRY
                     WRLEYQPKLQRSEADLVDELEEKVEEALRLHLVSDVPVGAFLSGGLDSSLLVAMLAKR
                     VGVSHLPTFTVGIPYPRFDEAPHARQVARLYGTEHHERMVAPSLVSLLPDLIYHLDEP
                     SDPLSLCTYHVAELARRHVKVVIGGDGGDELFGGYDRYYGNAYAGRYARVPAGLRRSV
                     LGPALALFPEAGWYKSIGHQ"
     gene            complement(1238..2410)
                     /locus_tag="VI195_02960"
     CDS             complement(1238..2410)
                     /locus_tag="VI195_02960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6823373.1"
                     /translation="MFCNYEYPPLGGGGGVVMAALARTLARRHEVTVLTSRMAGLPAV
                     SMDEGATVVRVPVLFRRQLAVANLPSMLAYLPSGLWRGLQLGRERRFDVINTHFALPS
                     GPVGDWLSNRLAVPNVLSVHGGDLFDPSKKMSPHRHAVLRAVVARLLRRADVVVAQSQ
                     DTASNVARIYGVDRSVELIPLGIDRPPRHEGGAQRAEFDLPANAFVLVTAGRIVARKA
                     CTQLIDTLGAVSGAVLLVVGDGPEAAALMRRAVECNVADRVRMLGYVTDAVKYRAYQV
                     ADVFVSTSQHEGFGLVFLEAMAFGLPIVCYDRGGQTDFLTSPSTGAVVPLNDLGAFNE
                     AVRSLRDAPERRAAIRRTNLLRVEDYFISRCAERYERVFERIVLAKRAAGTYQARA"
     gene            2500..3276
                     /locus_tag="VI195_02965"
     CDS             2500..3276
                     /locus_tag="VI195_02965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6823374.1"
                     /translation="MSVNYRASPLQASIEPRRLAARLEPFDSYWQAPKDAEKGFDAFA
                     AYYRSNYLPWMPQDRAARILVISCGPGYLLKMLQDHGYAAAFGIDSDPAKIAIAQRKS
                     LPCEVAHAFEFLDSHATQFDVIIPEQELNHLTLDETIEFLQRCRRALRPGGRIVVYAM
                     NGANPLVGSENLSHNIDHFYNVTEYSLEQLLKLGGFHNIRVFALKLYVFWTNPLNYVG
                     LTVTASLEFVLRVIFRLYGKKVRVLSKKIAASATAPADPA"
     gene            complement(3287..3922)
                     /locus_tag="VI195_02970"
     CDS             complement(3287..3922)
                     /locus_tag="VI195_02970"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEY6823375.1"
                     /translation="MNERVAAAYRMVRTRLLHRCRSNGLKTIAITSPGAGEGKSVTAL
                     NLALSLARDPTVNVFLLDLDMRSPSMCKYLGVQPPVELLAYFTGSRTAAEVFFSLGPK
                     NLALAGSLASTDAASELLGAGRLEALLSYINSLCPNAIILLDLPPVLVTDEALLLAPR
                     VDATLLVVAEGRTRRDSLARAVTLLAEFKCIGVLLNRSSEHMGENYYYYYK"
BASE COUNT          782 a         1336 c         1258 g          682 t
ORIGIN      
        1 ctgatgcccg atgctcttgt accagcccgc ttccggaaac aaggcgagcg ccgggccgag
       61 aacgctgcgc cgtagcccgg ccggcacacg cgcatagcgg cccgcgtagg cgttgccgta
      121 gtagcgatcg taaccgccaa acagctcgtc cccaccgtca cccccgataa ccaccttgac
      181 atgccgtcgg gcgagctcgg cgacatggta ggtgcacagc gacaagggat cggacggctc
      241 gtccaggtga tagatcagat cgggcagcag actcaccagc gatggcgcca ccatgcgctc
      301 gtggtgctca gtcccgtaca gacgcgcgac ctgtcgcgcg tggggtgcct cgtcgaaacg
      361 cgggtacggt atgccgacgg tgaaagtggg caggtggctg acccctaccc gctttgccag
      421 catcgcgacc agtaggctcg aatcgagacc cccactaaga aaggcgccga ccggcacatc
      481 gctcaccaga tgcaggcgca gagcttcctc gaccttttcc tccagctcat cgaccagatc
      541 ggcttcggag cgctgcagtt tcggctggta ctcgagccgc caatagcggc ggataaccgg
      601 ctcccgctcc ggctcgagta ccagcaggtg cgccggcggc agcttgcgta tgccgcggaa
      661 catcgacagc ggcggtgcga tgaggcgcag cgccagatac tggtcgagtg ccgccagatc
      721 gagcgtacgc agcgtatcat cgaaagcgag cagcgccttg atctcggagg caaacgcaaa
      781 gccacgcgcg ctgtgcgcgt agtagagtgg cttctgaccg agatggtccc gtgccagcag
      841 caagcgtcgc cggcgcgagt cccacagggc gaaggcgaac ataccgcgca aatgctcgag
      901 caggcggtca ccgtacagct cgtagagccc gatgatcacc tcgcaatcgc tctgggtcag
      961 gaagcgctgt ccgcggttct cgagattggc tcgtagcgcg cgatagttgt agatctcacc
     1021 gttgcagacc agccagatcg atttgtcggc gttgctgagg ggctgacggc cgccggcgag
     1081 atctatgatg gacaatcgcc ggtgcccgag aatcgcgtgc gcgtcctgct cgactcctac
     1141 gccatcgggt ccacgatgcc gcaggaccgc gagcatgcgt tgcatgcctt cgcccagctc
     1201 gttactcgga cctacgatcc ccgcgattcc gcacatgcta agcgcgcgcc tgataggtcc
     1261 cggcggctcg tttcgccaac acgattcgct cgaatacgcg ctcgtaacgc tccgcacacc
     1321 ggcttatgaa gtagtcctcg acgcgcagca ggttggtgcg ccggatcgcc gcgcgacgct
     1381 caggagcatc gcgcaggctg cgcaccgcct cgttgaaggc gcccagatcg ttgaggggta
     1441 ctacggcgcc ggtagacggg ctcgtcagaa agtcagtctg tccaccgcgg tcgtaacaca
     1501 cgatcggcag tccaaaagcc atcgcctcga ggaacaccag tccaaatcct tcatgctgac
     1561 tcgtcgagac gaacacatcc gccacctggt aggcgcggta cttcaccgca tcagtgacat
     1621 agccgagcat gcggacccgg tcggccacgt tgcactcgac ggcgcggcgc atcagcgcgg
     1681 ccgcttcggg tccatcgccg acgaccagca ggacggcgcc ggaaaccgcg ccgagcgtat
     1741 cgatgagctg cgtgcacgcc ttacgcgcca caatcctgcc ggccgtgacc agcacgaagg
     1801 cgttcgcggg gaggtcgaac tccgctcgtt gcgcgccgcc ctcgtgcctg ggtggtcggt
     1861 cgatgccgag cggaatcagc tcgacgctgc gatcgacgcc atagatccgc gccacgttgc
     1921 tggcggtgtc ctgcgattgg gccaccacga catccgcgcg gcgcaagaga cgcgctacca
     1981 cggcccgcag aacggcgtga cgatgcgggg acatcttctt cgaagggtcg aacagatcgc
     2041 ccccgtgcac cgacagtaca ttgggtaccg cgagccggtt tgacagccag tcgcccaccg
     2101 gcccgctcgg caaagcgaaa tgggtgttga tgacatcaaa gcgccgctca cgtccgagct
     2161 gcaggccgcg ccacaggccg ctcggcagat acgccagcat cgacggcagg tttgctaccg
     2221 caagctgcct gcgaaagagt accggcaccc gcacgaccgt ggcgccctcg tccatgctca
     2281 ccgccggcag cccggccatg cgcgaagtga ggaccgtcac ctcgtgtcgg cgcgcgagcg
     2341 tgcgcgccag cgccgccatg acgactccgc caccgccacc gagcggcgga tactcatagt
     2401 tgcagaacaa aacgcgcaat ctgtaatgag cgtcctgtta gtgtgagtgc atgtacgctc
     2461 aacgaccgct agtataagcg gaaattcacg aggtctccga tgagcgtcaa ctatcgcgcg
     2521 agtccgctcc aggcttcgat cgagccgcgc cgcctcgccg cccgcctcga gccgttcgac
     2581 tcctattggc aggcgcccaa ggatgccgag aagggcttcg atgcctttgc cgcgtactac
     2641 cgcagcaact acctgccgtg gatgccgcag gatcgcgctg cgcggatact tgtcataagc
     2701 tgcggacccg gctacttact gaagatgctg caggatcacg gctacgcggc ggccttcggc
     2761 atagattccg atccagcgaa gatcgctatc gcacagcgca agtcactgcc ctgcgaggtg
     2821 gcgcacgcct tcgagttcct cgatagccac gcaacccagt tcgacgtcat catcccggag
     2881 caggaactca atcatctgac gctcgacgaa accatcgagt ttctgcagcg ctgccggcgc
     2941 gcgctacggc ccggcgggcg catcgttgtc tacgccatga atggtgccaa cccgctggtg
     3001 ggctcggaga acctgtcgca caacatcgat cacttctaca acgtgaccga atacagcctc
     3061 gagcagctgc tgaagctcgg tggcttccac aacataaggg tttttgcgct gaagctgtac
     3121 gtcttctgga cgaacccact caactacgtg ggcctgacgg tgaccgcgtc cctcgagttc
     3181 gtgctgcgtg tcatcttcag actctacggc aagaaggtgc gcgtgctgag caagaaaatc
     3241 gccgcgagtg ccaccgcgcc cgccgatccg gcatgacccg gcgctgctac ttgtagtaat
     3301 agtagtagtt ctctcccata tgctcgctcg agcggttgag cagcaccccg atacatttga
     3361 attccgcgag cagggtgacc gcccgcgcaa gactgtcgcg gcgcgtgcgc ccttcggcaa
     3421 cgaccagcag ggtcgcatcc acgcgcggcg ccagcagcag cgcctcgtcc gtcaccagca
     3481 ctggcggtag atccagaaga atgatcgcgt ttggacacag cgaattgatg tagctcagca
     3541 aagcctcgag tcggccggcg cccaacagct cggacgcggc atcggtactg gcaagactgc
     3601 cagcaagcgc gaggttcttc ggcccgaggg agaaaaacac ctcggccgcc gttcggctgc
     3661 cggtgaaata ggcgagcagt tcgactggcg gttgcacgcc gagatacttg cacatgctcg
     3721 ggctgcgcat gtccaggtcg agcaggaaca cgttgacggt cggatcgcgc gccagactca
     3781 acgcgagatt gagcgcggtg accgacttcc cctcgcccgc gcccgggctg gtaatggcga
     3841 tggttttgag gccattgctg cggcacctgt gcagcaggcg cgttcgcacc atgcggtatg
     3901 cggccgccac ccgctcattc acgccggcca gtgtgtcggg cactagaatt cggtgcgcgg
     3961 cggctacggt gtgcgaacag ttgaccaccg ggaagggagg caatgcgact gtgaccacat
     4021 gcgcgggtgg gttgttttgc ctgccaggcg aatcctgc
//
LOCUS       DATDMP010000178         4325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2138734, whole genome shotgun sequence.
ACCESSION   DATDMP010000178 DATDMP010000000
VERSION     DATDMP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138734"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..2381)
                     /locus_tag="VI195_02975"
     CDS             complement(210..2381)
                     /locus_tag="VI195_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY6823376.1"
                     /translation="MSNAALRQRSSSRRYAAPLLESRWSIGMAAAAVLLTALAVPPPA
                     RAAGVRAVDSKSLLAASDNRAEWLTHGRTYDEQRFSPLAQIDTANVKHLGLAWFADLD
                     TARGQEATPLVIDGAIYTTTAWSEVKAYDAVSGKLRWEYDPKVPRAADVKLCCDVVNR
                     GLAAWGQRLYFGTVDGRLIALDRDTGRLVWSKLIVDPSKPYGITGAPRVIGGRVIIGN
                     AGADLGVRGYVAAYDGNDGRELWRFYTVPDRAGTNTAPHLQRAEHTWQGEWWTLGGGG
                     TVWDSMAYDPQLDLLYIGVGNGSPWNQNYRSPGGGDNLYISSIVALKPQTGAYVWHYQ
                     TTPGDSWDFDATQHMILADLEIGGRLRKVLMQASKNGFFYVLDRANGQLISANNFVTV
                     NWAKGVDIESGRPIENPEARIERTGKLYVVVPGAGGAHSWQPMAYDPQTGLVYIPALE
                     AGFPYMPDAHWKPAALGFNTGMDLAAGAMPADINIRSAVKAATKGALIAWDPIAQKER
                     WRVPFKGPWNGGVLATAGGLVFQGNAAKEFAAYDAAGGAKLWSMGVQTGIIAAPVTYS
                     INNEQYVAVLAGWGGIWALAPGILSEVAGPISNVSRLLVFKLNGTAQLPPEHPLPQRA
                     LAPPAASGTPEQIAEGTRQYARFCGVCHGDAAYGGGLLPDLRRSALLSDGAAWSSVVR
                     DGALRTRGMVSFAPVLSAEQVEAIRHYVIKRANQDKALGDR"
     gene            2568..2987
                     /locus_tag="VI195_02980"
     CDS             2568..2987
                     /locus_tag="VI195_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6823377.1"
                     /translation="MSNPEPRYTGGCLCGAVRYEAEGEPSLMGHCYCADCRKASGSGF
                     IPFMGFTGAAVRFSGKTREFSCQAASGRQAVRNSCPVCGGLVFGGRVGQDDSFTIYAG
                     SLDDASLFRPQVAIFTRDRPEWAVIPAGLKVYAGLPG"
     gene            3239..4261
                     /gene="speB"
                     /locus_tag="VI195_02985"
     CDS             3239..4261
                     /gene="speB"
                     /locus_tag="VI195_02985"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY6823378.1"
                     /translation="MDIEKLKRLRARYADRGAFDGDDPVLLRAARDVQTGDGRRSLPY
                     SGVPTFLGLPHATSLDGLDIAVVGVPMDLGVSNRAGARFGPRAVRTIERIGPYHPTFR
                     GVPKGRARAADIGDVTFRSRYDLEQSLKDIEEYYLALRARGVRPLSVGGDHSITYPIL
                     KALGKDAALGLVHIDAHCDTMGAYDDSKFHHGGPFRLAVLDGVLDPDRTIQIGIRGAA
                     NMFWEFSHASGMTVIYMEDFMRMGVAAVIDKAREVVGDLPIYISVDVDGFDPVFAPGT
                     GTPEPGGLSSREGLALLRGLRGLKIAGADVVEVAPQYDSTTNTSQLAAQILFEEFALM
                     ALGEAE"
BASE COUNT          718 a         1421 c         1407 g          779 t
ORIGIN      
        1 agcgctgact gaaaacgcgg caagtgttcg ctttgggatc ccaattgtca attcagcgac
       61 aaaggctgca agccatttgc atcaaatctc gcttgataag ttcttgattt agaatactta
      121 gctttcagcg cgcgcgagca cgattgccgg tcattgccta caaacaacga cccgttggga
      181 tcccacagtc ccgagcggac gccgcccggt tatcgatcgc ccagcgcctt gtcctggttc
      241 gcacgcttga tgacgtagtg acggatcgcc tcgacctgct cagcactcag gacaggagcg
      301 aagctgacca ttccccgagt gcgcagagcg ccatcgcgca ccacgctcga ccaggccgcg
      361 ccgtcggaga gcagcgcgct gcggcgcagg tccggcagca gtccgccgcc gtacgccgca
      421 tcgccatgac aaacgccgca gaaccgggcg tactgtcgcg tcccctcggc gatctgctcg
      481 ggagtgccac tcgcggcagg gggcgcgagc gctcgctgcg gcagcggatg ctcggggggc
      541 agctgtgccg tgccgttcag cttgaagacg agcagtcgcg acacgttgct gatgggcccc
      601 gcaacctcgg aaagaatgcc gggtgccagt gcccagatgc cgccccaacc cgcgagcacc
      661 gcgacgtact gctcgttgtt gatcgagtag gtgacgggag cggcgatgat gccggtctgc
      721 acacccatcg accacaattt cgcgccaccc gcggcgtcat aggcagcgaa ctccttggcg
      781 gcgttaccct gaaacacgag tccaccggcg gtcgccagca caccaccgtt ccacggtcct
      841 ttgaagggca cccgccagcg ctccttttgc gcgatcgggt cccaggcgat gagcgcgccc
      901 ttggtggctg ccttcaccgc tgagcggatg ttgatatccg ccggcatcgc accggccgca
      961 aggtccatgc cggtgttgaa gcccagcgct gccggtttcc agtgggcgtc gggcatgtag
     1021 gggaagccgg cttcgagcgc cggaatgtag acgagcccgg tctgcgggtc gtacgccatc
     1081 ggttgccagc tgtgcgcgcc accggcaccc ggcactacga catacagctt accggtcctc
     1141 tcgatacgcg cctcgggatt ctcgatgggc cgtccgctct cgatgtcgac gcccttcgcc
     1201 cagttgacgg tcacgaagtt attggcggag atcaactgtc cgttggcgcg gtcgagcacg
     1261 tagaagaagc cgttcttcga tgcctgcatg agtaccttgc gcagccggcc gccgatctcg
     1321 agatccgcga ggatcatgtg ctgcgtggca tcgaagtccc acgaatcgcc gggcgtcgtc
     1381 tgatagtgcc agacgtatgc tcccgtctgc ggcttcagcg cgacgatcga ggagatgtag
     1441 agattgtcgc caccgccggg tgagcggtaa ttctggttcc agggactgcc gttacccaca
     1501 ccgatgtaga gcaggtcgag ctgcgggtcg taggccatcg agtcccacac cgtgccgccg
     1561 ccgccgagcg tccaccattc gccctgccag gtatgctcgg cgcgctgcag gtgtggtgcc
     1621 gtgttggtgc cggcgcggtc gggcaccgtg tagaagcgcc acaactcgcg gccatcgttg
     1681 ccgtcgtacg cggccacgta gccgcgcacg cccaggtcgg caccggcatt accgatgatc
     1741 acgcggccgc cgatcacccg cggcgcgccc gtgatgccgt agggtttgga gggatccacg
     1801 atgagcttcg accagacgag cctgcccgtg tcgcgatcga gcgcgatcag acgcccatcc
     1861 acggtgccga agtagaggcg ctggccccag gcggcgaggc cgcgattaac gacgtcgcag
     1921 cagagtttca cgtccgcggc gcgcggcacc ttcggatcgt attcccagcg caacttgccc
     1981 gagaccgcgt cgtacgcctt cacttccgac caggcggtcg tggtgtagat ggcgccgtcg
     2041 atgaccagcg gtgtcgcctc ctggccgcgt gcggtgtcga gatctgcgaa ccaggcgagg
     2101 cccagatgtt tcacgttggc ggtgtcgatc tgcgcaagcg gcgagaagcg ctgctcatca
     2161 taggtacgac cgtgcgtgag ccattcggcg cgattgtcac tcgctgcgag caatgacttg
     2221 ctgtcgaccg cacgcacgcc cgcggctcgt gcaggcggcg gcactgcgag cgcggtgagg
     2281 agtacggcag ccgctgccat gccgatcgac cagcggctct cgagcaacgg cgctgcatat
     2341 cgacgcgaag atgaccgctg acgcagcgcc gcgttgctca tcgccttccc cggttcttgt
     2401 aagcgttgca taccattggc cgagtcggcg ggtcaagcga gtgcgcggcc ggcctgatgc
     2461 acacgcgctg atctcaatgg cgtcgcgcct cgttggaagc tggagcgcca cttcgctttg
     2521 caggcgccgc gcttgccggc cttcaggcca cgaccaataa tcgagccgtg agcaacccgg
     2581 agccgcgata caccggcgga tgtctctgtg gcgcggtgcg ctacgaggcc gagggagagc
     2641 cttccctcat ggggcattgt tactgcgcgg attgccgcaa agcctcgggc tcgggattca
     2701 ttccgttcat gggattcacg ggcgccgccg tgcgcttcag cggcaagacg cgggaattca
     2761 gctgccaggc ggcgagcggt cggcaggccg tgcgcaactc ctgtccggtg tgcggcggtc
     2821 tggtgttcgg cgggcgcgta ggccaggacg acagtttcac catttacgcc ggctcgctcg
     2881 atgacgcgtc cttgtttcga ccgcaggtcg ccatcttcac gcgcgaccgc cccgaatggg
     2941 cggtgatacc cgcgggtctg aaagtctacg caggattgcc cggctgatcg gcaaacctgt
     3001 cagaagttgc cccgcggcag agatttcttt cagcgtaccc gagcactggt tcgactccac
     3061 ctccctgccg aagccggagg cgcactgacg cggcaccccg gcacgaatcc ctgctcgagt
     3121 gcagtttccg cgcagatgcc ctgccgcgta ggccgtttac tgctcagtca cgcccaagcg
     3181 ctgctggctt tctacttgcc cggatccgtc gtagattggc gagcgggaga cgaagcctat
     3241 ggacattgaa aagctcaaaa gactgcgggc gcgctacgcg gatcgcggtg cattcgacgg
     3301 cgacgacccg gtgctgctgc gggctgcgcg tgacgtgcag accggcgatg gcagacgctc
     3361 cttgccgtat tccggcgtgc ccacctttct cgggctgccg cacgcgacga gcctcgacgg
     3421 actggatatc gccgtcgtcg gggtccccat ggacctcggc gtgtccaatc gcgccggggc
     3481 gcgctttggt ccgcgcgcgg tgcgcaccat cgagcgcatc ggaccctacc atccgacgtt
     3541 tcgcggcgtg cccaaggggc gtgcgcgcgc ggcggatatc ggcgatgtca cctttcgcag
     3601 ccgctacgat ctcgagcaat cactcaagga catcgaggag tactacctcg cgctgcgcgc
     3661 acgcggcgtg cggccgctat cggttggcgg agatcactcg atcacctatc caatcctcaa
     3721 agcgctgggt aaggacgctg cgctcggcct ggtgcacatc gatgcgcact gcgacaccat
     3781 gggcgcctac gacgacagca agttccacca cggcggaccg ttccgcctcg cggtcctcga
     3841 tggtgttctg gacccggacc gcaccatcca gatcggcatc cgcggtgcgg cgaacatgtt
     3901 ctgggagttc tcgcacgctt ccgggatgac ggtcatctac atggaggatt tcatgcgcat
     3961 gggtgtggcg gcggtcatcg acaaggcgcg cgaggtggtc ggagatctgc ccatttacat
     4021 ctcggtggat gtcgatggct tcgacccggt gtttgcgccg ggcaccggca cacccgagcc
     4081 tggcgggctc agctcacgcg aaggtctcgc gctgctgcgc ggcttgcgcg gcttgaagat
     4141 cgcgggtgcc gatgtagtcg aggtcgcgcc ccagtacgac tccaccacca acacgagcca
     4201 gctcgcagcg cagatactat tcgaggagtt cgcgctgatg gcgctcggcg aggccgaata
     4261 gcccgcggcc tctacgggtc gtcactgccc ggcgactccg cggatctagc catggttggg
     4321 cggct
//
LOCUS       DATDMP010000179         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1694375, whole genome shotgun sequence.
ACCESSION   DATDMP010000179 DATDMP010000000
VERSION     DATDMP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694375"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /gene="fmt"
                     /locus_tag="VI195_02990"
     CDS             <1..672
                     /gene="fmt"
                     /locus_tag="VI195_02990"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY6823379.1"
                     /translation="YGLILPRSVLDIPERGCINIHASLLPRWRGAAPIQRALLAGDAE
                     TGISIMKMESGLDTGPVLARRAVAIASEEDAGTLHHKLADVGAMLINEVLARLDDALA
                     RAEPQDDGQATYAAKIQKKETLLSWSEPAARLERMVRAFRPAPGAQTVAGQETLKIWR
                     ARVADGRGRPGTVLHAEEEIVVACGEQALAITELQRAGGKRLTAAAFLAGHPLAAGSR
                     LGTQE"
     gene            669..1946
                     /gene="rsmB"
                     /locus_tag="VI195_02995"
     CDS             669..1946
                     /gene="rsmB"
                     /locus_tag="VI195_02995"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY6823380.1"
                     /translation="MKASGQLAHALSKSARIVAGVAAGRSASDASLVKDATEASGSRA
                     AIMDLTHGTLRRYGRVQATMRLLSSRGTAQDVVQALLWCSFYALDSGRYADYTVVDQA
                     VNACALLHADNAKGYVNALLRTYLRQRQSMERRLGDDVEALFQHPRWWIDIVRAAYPA
                     QYEAILAAGNAHPPMCLRVNRRRTCAPAYARRLEAVGVASRLLGDEALLLPQPVPVDR
                     LPGFELGEVSVQDAGAQRAVRCLELETGQIVLDACAAPGGKAAHILESADVALTAIDI
                     DGVRCQRVARDLQRLGLAAKVAVGDCTRPDEWWDGILFHRVLADVPCSASGVARRHPD
                     IKWLRRASDIASFAARQSAMLDALWRLLAPGGKLLYVTCSLFPGENEEVVSAFLTRAP
                     GAHRRPLSDGEPAQWFPTSEHDGFFYALIEKAA"
     gene            1947..2567
                     /locus_tag="VI195_03000"
     CDS             1947..2567
                     /locus_tag="VI195_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY6823381.1"
                     /translation="MRRVLLRTAAFAPFLAVALLFQATAFAEDIEVREAQLHAVEEGL
                     ALDADFAFDLTPRLADVVANGVPLYFQVEFELTRPRWYWFEEKTASKRLQLRLSYHAL
                     SRQYRLSTGLLQQSFPTLEEALNVLKRVRNWVVVDRSVGLSDSDYEAAVRMRLDTTLL
                     PKPFQLSALTSRELHLESAWKRFTVPAPEHAPAPVENREQKAPQDR"
     gene            2564..>3088
                     /locus_tag="VI195_03005"
     CDS             2564..>3088
                     /locus_tag="VI195_03005"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY6823382.1"
                     /translation="MKLLLIIGSTVAAIGLFLLASASGDTAFFARHYPTLVAVNAALA
                     VLLAAIVAYQLLSLVRRYRAGVFGARLTVRLLARFALLAVVPGLIVYAVSVQFLTRSI
                     ESWFDVKVDAALEGGITLGQHVVDQMLLDLQTKGRGMALDLADRSAPQLGARLERLRE
                     QTGVEEAVLITANSR"
BASE COUNT          497 a          990 c         1019 g          582 t
ORIGIN      
        1 tacggcctga tcctgccgcg ctcggtgctc gatattccgg aacgcggctg catcaatatc
       61 cacgcgtcgc tgctgccgcg ctggcgcggc gcggcgccta tccagagagc cctcttggca
      121 ggggatgccg agaccggcat ctcaatcatg aaaatggaat ccggcctgga cacggggccg
      181 gtactcgccc gacgagctgt ggctatcgca tccgaagagg atgccggtac gcttcaccac
      241 aagctggcgg atgtcggcgc catgctcatc aacgaggtct tggcgcggct cgatgatgct
      301 cttgcccgcg ccgagccgca ggacgacgga caagcaacct atgcggccaa gatccaaaag
      361 aaagaaacgc ttctttcctg gagcgagcca gccgcccgtc tcgagcgcat ggtgcgcgct
      421 tttcgcccgg cgcccggagc tcagaccgtc gcgggtcaag aaaccctgaa gatctggcgc
      481 gctcgcgtgg ccgacggacg aggcaggccg ggaaccgtgc tgcacgcgga agaggaaatc
      541 gtggtcgctt gcggcgagca ggccttggcc atcacggagt tgcagcgggc cggcggcaag
      601 cgcctcaccg ccgcagcgtt tctggccgga catcctctcg ctgcgggatc gcgcctcggc
      661 acgcaagaat gaaggcgagc ggtcagcttg cccatgcgct cagcaagtcg gcgcgaatcg
      721 ttgccggtgt agcagcgggc cgaagcgcgt cggatgcgtc cctggtgaag gatgcaaccg
      781 aggcctcggg ctcgcgggcc gcaatcatgg atctcacgca cggtacgctg cgccggtacg
      841 gtcgtgtcca ggcaactatg cgtttgctct cctcgcgtgg caccgcgcag gacgttgtgc
      901 aagcgctcct ttggtgctcg ttctacgcct tggattctgg ccgctatgcc gactatacgg
      961 tcgtcgacca ggcggtgaac gcgtgcgcgc tgctacatgc ggacaacgcc aagggatatg
     1021 tcaacgcgct gctgcgcacg tacttgcggc agcgccagtc aatggagcgt cggctcggcg
     1081 acgatgtcga agccctcttc cagcatccgc gctggtggat tgacatcgtg cgcgcagcgt
     1141 atcctgcgca atacgaggct atcctcgccg cgggaaacgc gcatccgccc atgtgcctgc
     1201 gcgtcaaccg tcgccggact tgcgcgccag cgtacgcgcg ccgattggaa gctgtcgggg
     1261 ttgcgtcgcg cttgctcggt gatgaggcct tgcttctccc ccagccggta cctgtcgatc
     1321 gcctgccggg tttcgagctc ggcgaagtat ccgttcagga cgcgggcgca cagcgcgcag
     1381 tgcgctgcct cgagctcgag accggtcaaa tcgttctcga cgcctgcgcg gcgcccgggg
     1441 gaaaggctgc gcacatcctc gagagtgccg acgtcgccct caccgcgatc gatattgacg
     1501 gcgtgcgatg ccagcgcgtc gcgcgcgatc tccaacgcct cggcctcgcg gcaaaggtcg
     1561 ccgtgggcga ctgtactcgt cccgatgagt ggtgggacgg catccttttt catcgcgtgc
     1621 tcgccgacgt accgtgttcg gcttccggcg tggcacgccg ccatccggat atcaaatggc
     1681 tgcgccgggc gagcgacatt gcaagcttcg cggcgcggca atcggccatg ctggacgcgc
     1741 tttggcggct gctcgcgccg ggtggtaaat tgctatatgt cacctgctcg ctctttccgg
     1801 gggaaaacga agaggtggtt tctgcattct tgacccgagc gccaggcgcc catcggaggc
     1861 cattgtccga tggcgagccc gcgcagtggt ttccgacttc cgagcacgac ggcttctttt
     1921 acgcgctgat agaaaaagcg gcctgaatgc gccgcgtcct gctacgcacc gcagctttcg
     1981 cgccttttct cgcggttgcg ctgctgtttc aggcaacggc cttcgccgag gacatcgagg
     2041 tgcgcgaagc gcagctgcac gccgtggaag agggtctcgc ccttgacgcg gacttcgcat
     2101 tcgatctcac gccgagattg gccgacgtgg tggcgaatgg agtaccgctc tatttccagg
     2161 tggagttcga gcttacccgt ccccgctggt attggttcga ggaaaaaacc gcctcgaagc
     2221 ggctgcagtt gcggctgtcc taccacgcgc tttcccggca ataccgcctg tcgaccgggc
     2281 tcttacagca gagctttccc acgctcgagg aggcccttaa cgtattgaaa cgcgtgcgca
     2341 actgggtggt cgtggatcgc agcgtcgggc tttcggactc cgactatgaa gccgcggtgc
     2401 gcatgcgtct cgatacgacg ctgttgccga agcccttcca gttgagtgcc cttacgagcc
     2461 gcgagttgca tctggaatcg gcctggaagc gcttcacggt ccctgccccc gagcacgcac
     2521 cggcgccggt cgaaaatcgc gagcagaagg cgccgcagga ccgatgaagc tgctgctcat
     2581 cataggaagc acggtcgcgg ccatcgggct tttcctgctg gccagcgcga gcggggacac
     2641 cgctttcttt gcacgccatt atccgacgct ggtggccgtg aacgcagcgc tggcggtgct
     2701 gcttgccgcc atcgtggcgt atcagcttct gtctctggtg cgtcggtacc gggccggggt
     2761 gttcggcgcg cgcctgaccg tccggctgct ggcaaggttt gcgttgctcg cggtggtgcc
     2821 ggggctgatc gtatacgccg tctccgttca gttccttacc cgctcgatcg aatcatggtt
     2881 cgatgtgaaa gtggacgccg cgcttgaggg cggcattacg ctgggccagc atgtggtcga
     2941 ccagatgctt ctcgacctgc agacgaaggg gcgcggcatg gccctcgatc tcgcagaccg
     3001 ctccgcgccg cagctcggcg cgcgcctgga gcggctgcgg gagcagaccg gggtggaaga
     3061 ggccgtcctg atcacggcga acagccgc
//
LOCUS       DATDMP010000180         3262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_361126, whole genome shotgun sequence.
ACCESSION   DATDMP010000180 DATDMP010000000
VERSION     DATDMP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361126"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..931
                     /locus_tag="VI195_03010"
     CDS             194..931
                     /locus_tag="VI195_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6823383.1"
                     /translation="MSLIEDLVIASRTLAEHGVIDGYGHASVRSDNDPKHYFMARAVA
                     PELITEDDILEFDLDSNPLDQRGRAVYNERFIHGEIYKLRPEINAIVHNHSPTVVPFA
                     CTHTTLQPIFHMSAFIGLGVPTWDIRDAQQGTDLLVRTPALGRSLAEGLGKHPAALMR
                     GHGSVVVGENLQRAVGRTIYLEMNARMQLNAQVLAGPGGEIVFMDDKEVAANVSWQNY
                     DRAWHLWKTKALAKLEAERALAASART"
     gene            complement(932..1600)
                     /locus_tag="VI195_03015"
     CDS             complement(932..1600)
                     /locus_tag="VI195_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6823384.1"
                     /translation="MRLFQGLPEDTLHSIASRCAWRRCPAGELIVSRETREHEVYLLV
                     SGTARVTAFSASGRQVTYRDLSAGDWFGDLAAVDHRPRSADVVAQTEALVACMRGGDF
                     IDLVLQSPQVARALLEHLVQRVRELTDRVFELSTLGVQNRIDAEVLRLAKSAGVTRNA
                     ARIDPAPLHADVASQVSTNREQVTREISQLVKLGIVSRDGKALVVPDVARLERLVAEV
                     RRTA"
     gene            1844..2089
                     /locus_tag="VI195_03020"
     CDS             1844..2089
                     /locus_tag="VI195_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823385.1"
                     /translation="MTRTFHLHAASTVTVIAAALASAIAVGLLAAVVTLFERDGYPME
                     RVLVAERACAGSTYVSEREVCMREWLATYRIQHVAQK"
     gene            2137..2628
                     /locus_tag="VI195_03025"
     CDS             2137..2628
                     /locus_tag="VI195_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6823386.1"
                     /translation="MKRISRIAAALLFALGCSTAVADTVADKDCPKMLQVRGVHEVMT
                     AQITLFKAGPRALACNYDQDAVLLLPGVAAAGRQQIEAAYTSLFEAYGGDLAIEVNSL
                     TESDSAALMDYTIVSGNDVVLRGVDTFVIEAGLVKVQSHAVAGTSGVPRLNNGLGPRR
                     YHE"
     gene            2712..2787
                     /locus_tag="VI195_03030"
     tRNA            2712..2787
                     /locus_tag="VI195_03030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2745..2747,aa:Lys,seq:ctt)
     gene            complement(2793..>3262)
                     /locus_tag="VI195_03035"
     CDS             complement(2793..>3262)
                     /locus_tag="VI195_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY6823387.1"
                     /translation="QANVLHLKWSQVDLERRTAWIHADEAKGREAIGVPLNDEAMAVL
                     REELGKHKDAVFTYRRKPLNSVNTKGWKAGLKRAGIDNFRWHDLRHVWATWHVMAGTS
                     LGELQELGAWKSETMVKRYAHFAPEHLRRAADRLATFWYTGKKTGCVGEDATC"
BASE COUNT          581 a         1050 c         1045 g          586 t
ORIGIN      
        1 ctcaatgcgg aaatcgatga ggcgctgcag ttgcccgaag tgcgcaacgc gatggcacag
       61 cagggcatga cgcccgtcat cgccggcccc gagcgcatgg ggcaggtgct cgtgagtgag
      121 ctcgcgcgct ggaagcgcgt cgttgccgat gccggcatcc agatcgaata gtcgccgtcc
      181 cggagaacgc aacatgagcc tgatcgagga tctcgtcatc gcatcgcgca cgttggccga
      241 gcatggcgtc atcgacggct acggtcacgc ctcggtgcgc tcggacaacg atcccaagca
      301 ctacttcatg gcgcgcgcgg tggcgccgga gctcatcacc gaggacgaca tcctggagtt
      361 cgatctcgac agcaatccgc tggatcagcg tggacgcgcc gtgtacaacg agcgcttcat
      421 ccatggcgag atctacaagc tgcgaccgga gatcaacgcc atcgtgcaca accattcgcc
      481 gaccgtcgta ccgttcgcgt gcacgcacac cacgttgcag ccgatcttcc acatgtcggc
      541 gttcatcggg ctcggcgtgc cgacgtggga catccgcgac gcgcagcaag gcaccgatct
      601 tctggtgagg acgccggcgc tcggccgttc cctcgccgag ggcctgggca aacatccggc
      661 agcgctgatg cgtggccatg ggtcggtcgt cgtaggcgag aacctccaac gcgcggtggg
      721 ccgcacgatc tatctggaga tgaatgcgcg catgcagctc aatgcgcaag tgctcgcggg
      781 accgggcggc gaaatcgttt tcatggacga caaggaagtc gccgccaacg tttcgtggca
      841 gaactacgat cgcgcctggc atctgtggaa gaccaaggcg ctcgcgaagc tcgaagccga
      901 acgcgccttg gcagcgtccg cgcgcacctg atcaggcggt gcgccgcacc tccgccacga
      961 ggcgctccag gcgcgccacg tccggcacca ccagcgcttt gccgtcgcgc gacactatgc
     1021 ccagcttcac gagttgcgag atctcccgcg tcacctgctc gcgattcgtg ctcacctgac
     1081 tcgccacatc ggcgtggagc ggcgcagggt cgatgcgtgc agcattgcgc gtgacacccg
     1141 cactcttggc gagccgcagc acttcggcat cgatgcgatt ctgcacgccg agggtcgaga
     1201 gctcgaacac gcggtcggtg agctcgcgca cgcgctgaac gaggtgctcg agaagtgcgc
     1261 gcgcaacctg gggcgactgc agcacgagat cgatgaaatc gccgccgcgc atgcacgcga
     1321 cgagtgcttc ggtctgtgcg accacatcgg ctgagcgggg acgatgatcg accgcggcga
     1381 gatcgccgaa ccagtcgccc gccgagagat cgcgatatgt cacctgccgc ccgctcgccg
     1441 agaaggcggt cacccgcgcc gtaccgctga cgagcaggta gacctcgtgc tcgcgcgttt
     1501 cgcgcgacac gatgagctcg ccggcgggac atcgacgcca ggcgcagcgg ctcgcgatgc
     1561 tgtggagagt gtcctcggga aggccttgga aaagccgcac cgatcgcaat ccgacgatgg
     1621 atggcgtaac gccggcagat gacgagtgcg tggccacggc gatcgtgagg gctcctttca
     1681 ggctgcgcta ttggataacg actcgggccg cggggcaagg cccgcctctg ccccgacggc
     1741 ccacgagcgt ggcagcgacc tgcggcaggg tgtgcctttt cgcacatccg gcgcctgcgt
     1801 taccgcggaa cattcgaatc gtgcttttga cgggagattc gaaatgacac gcaccttcca
     1861 ccttcacgcc gccagcactg tcaccgtcat cgcagcggcg cttgcaagcg caatcgcggt
     1921 cgggctgttg gcggcggtgg tcacgctgtt cgagcgagac ggctatccga tggagcgcgt
     1981 gctcgtcgcc gagcgggcgt gtgccggcag cacttatgtg tcggagcgcg aagtctgcat
     2041 gcgtgagtgg ctcgccacgt atcgcatcca gcacgtcgca cagaaataat ttcgggcgcc
     2101 gtcaagcgcg gcgccctttt taccacggag gtattcatga aacggatatc acgtatcgca
     2161 gcggccctct tgttcgcact gggctgcagc acggcagtcg cagacacggt tgcggacaag
     2221 gactgcccga agatgctgca ggtgcgcggc gtgcatgaag ttatgacggc gcagatcacg
     2281 ttgttcaagg ccggcccgcg ggcgctcgcg tgcaactacg accaagatgc ggtcctcctg
     2341 ctgccgggag tcgcggcggc ggggcggcag cagatcgagg cggcgtacac gtccctgttc
     2401 gaggcgtacg gcggcgatct cgccatcgag gtgaactcgc tcaccgaatc cgacagcgcg
     2461 gcactcatgg attacacgat cgtcagcggt aatgatgtcg tgctgcgcgg cgtcgatacc
     2521 ttcgtcatcg aggctggatt ggtgaaggtg caatcccatg ccgtggctgg aacgtccggt
     2581 gtccccaggc tcaacaacgg cctgggtccg cggcgctatc acgagtagcg ctcccacagc
     2641 tgatgcgggg cgggtgattt gcgttacaat tacggccctc gcgagtgtga ttcgtggcgc
     2701 tcgcggtgcg tgggccgtta gctcagctgg tagagcagcg gactcttaat ccgttggtcg
     2761 taggttcgat ccctacacgg cctaccaacc aatcaacagg ttgcgtcctc tccgacgcaa
     2821 cctgttttct ttcctgtata ccaaaacgta gcaagtcgat cagccgcacg ccgcaaatgc
     2881 tcgggcgcga agtgcgcgta acgcttgacc atcgtttcgg acttccatgc gccgagctcc
     2941 tgcagctcgc ctaagctggt gccggccatt acgtgccacg tggcccagac atgccgcagg
     3001 tcgtgccacc ggaaattgtc gatcccggca cgcttcagcc ctgccttcca gcctttggtg
     3061 ttcacgctgt tgagcggctt ccggcgatag gtgaaaacgg catccttgtg cttgccgagc
     3121 tcctcacgta ggacggccat cgcctcatcg ttgagcggca ccccgatcgc ctcccgtcct
     3181 ttcgcttcgt ccgcgtgtat ccatgctgtc cgccgctcga gatcgacttg ggaccacttg
     3241 agatgcagca cgttcgcttg gc
//
LOCUS       DATDMP010000181         4776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_138922, whole genome shotgun sequence.
ACCESSION   DATDMP010000181 DATDMP010000000
VERSION     DATDMP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138922"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VI195_03040"
     CDS             <1..470
                     /locus_tag="VI195_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6823388.1"
                     /translation="TRDSTFRKMTPDQWRAVLATNLDSVFNVTRHVIDGMVERGWGRI
                     INISSVNGQKGQFGQTNYSTAKAGIHGFTMSLAQEVAARGVTVNTVSPGYIGTDMVRA
                     IKPDRLEKIVSGIPVKRLGEPEEIASIVAWLAGDESAFATGADFSLNGGLHMG"
     gene            498..1484
                     /locus_tag="VI195_03045"
     CDS             498..1484
                     /locus_tag="VI195_03045"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6823389.1"
                     /translation="MKICVVGAGAIGCFLGTRLALERRCEVSALARGPTLVALRDHGW
                     RLQEGGELAQAPAACAADDPRELGPQDLLVIAVKGPALPALAPTLVPLLGADTVVLPA
                     MNGVPWWFGRGIPALEDRPLESVDPGGVITAALPISRVVGCVVHAAVSTAAPGIGVHR
                     MGHGLILGEPAGGESARVAQLGALFSRAGFEVRSSSHIRHDVWYKLWGNMTMNPVSAL
                     TGATLDRVLDDELVRNFCSAAMREAAAVGARIGCDVHESPEDRHAVTRRLGAAKTSML
                     QDVEALRPLELDALVGAVREIGARVGVATPGIDALFGLTRLFGQVRGLYPAQ"
     gene            2025..2639
                     /locus_tag="VI195_03050"
     CDS             2025..2639
                     /locus_tag="VI195_03050"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY6823390.1"
                     /translation="MHPAALVIFLTAAAGVYPSNTSDKPTISGTPATSASVGSPYAFE
                     PSAHDPYGAPLAFSINNKPAWANFSIATGSLYGAPTSAQAGTYANIEITVSNGKVSAA
                     LPPFSITVKNGATSTTTASATLSWTPPTENADGTALTDLAGVHIYYGNSAADLSQVVQ
                     VSGTDESSYTIKNLAAGTWYFAASAYTTTGAQSALSSVVSKSIP"
     gene            complement(2656..3852)
                     /locus_tag="VI195_03055"
     CDS             complement(2656..3852)
                     /locus_tag="VI195_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823391.1"
                     /translation="MAFVLRLALVPLRPPTAHTENIKAGFTLAQRGYLGDPFVIPTGP
                     TAHVSPAYPVLVAAVRSITPSDDACLHILAIILAVVSSCNIAALLPISRALKLPPASG
                     TIAALIWLIPFFAWVELSGEHETPFTVAALLAVVTVVIRTIERARARVATGAGLGFAA
                     GLAAYFTPTVLPVAAFVTLVGARLVGWKPRGLLALLAGATLAFAVAILPYTLRNHHVL
                     GKWFFMRDNFGLELAMSNGPNARATETENGAAGGTLQRHPFNSPATAASVRDLGEVEY
                     NHQMQRAALAWIKANPRAFLGLVAERLGYMVLPQSVRWYQRVIAGAISLMTIAGAVLL
                     WHSRYKYGIRCLTAAIAGYLLVYLLIEHDMRYMYPALFLESLIAASFAVVLLGMWRDR
                     DVADSA"
     gene            complement(4022..>4776)
                     /locus_tag="VI195_03060"
     CDS             complement(4022..>4776)
                     /locus_tag="VI195_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823392.1"
                     /translation="GIPGVVLALIVWLTVREPSRGQLDAGHLPGTRSTLTATLRFIAH
                     QRSAVHLLLGGSVATFWSWGLMWWTPAFLQRSHHLTVGQAGQLIGPMHLIAGTASTLF
                     AGWLMARRAADDPRYVTRLLGWVTALTTIPSLLLYWVVSERAAVALLWIFVPAVYFYI
                     GPILGLLQNVVPAQMRATTCAILLFLANIANLLLAPQLIGALSDWFAASFGAGTESLR
                     WALLLLAPTGFWAAWHLWTSGRTIRTDAARIG"
BASE COUNT          937 a         1521 c         1544 g          774 t
ORIGIN      
        1 tcacgcgcga ttccaccttc cgcaagatga cgcccgatca gtggcgcgcc gtgctcgcca
       61 ccaacctcga cagcgtcttc aacgtcacgc gccacgtgat cgacggcatg gtggagcgcg
      121 gctgggggcg catcatcaac atctcctcgg taaacggcca gaagggccag ttcgggcaga
      181 cgaactattc gacggccaag gcgggcattc acggcttcac catgtcgctc gcgcaggaag
      241 tcgcagcgcg cggcgttacg gtcaacacgg tctcgcccgg ctacatcggt accgacatgg
      301 ttcgcgccat caagcccgac cggctcgaga agatcgtgag cggtatcccg gtgaaacgcc
      361 tgggcgagcc cgaggagatc gcctccatcg tcgcgtggct ggcaggcgat gagtccgcgt
      421 tcgcaaccgg tgcagatttc tccctgaacg gtgggctcca catgggctga gccggtcgcc
      481 tcgagggagc ggcaccgttg aagatctgtg tagtcggcgc cggcgccatc ggctgctttc
      541 tcggtacgcg tctcgcgctc gagcgccgtt gcgaggtttc ggcactggcg cgcggtccga
      601 cgctcgtcgc gctgcgcgat cacggctggc ggctgcagga aggcggcgag ctggctcagg
      661 cgcccgcggc gtgtgcggcc gatgatccgc gcgagctcgg cccacaggat cttctcgtca
      721 ttgccgtaaa aggtcccgcg ctccccgcac ttgcgccgac gctcgtgccg ctcctcggag
      781 cggacactgt ggtgctgccg gccatgaatg gtgttccctg gtggttcggt agaggcatcc
      841 cggcgctcga ggatcgtccg ctcgagagcg tcgacccagg tggcgtcatt accgcagcgc
      901 tccccatatc acgagtcgtc ggctgcgtgg tgcatgccgc tgtgagcact gctgcgccgg
      961 gcattggagt gcatcgcatg ggacacggcc tcatcctggg cgagccggcg ggcggtgagt
     1021 ctgcgcgcgt cgctcagctg ggcgcgctct ttagccgcgc cggcttcgaa gtgcgcagct
     1081 cgagccatat ccgccacgac gtctggtaca agctgtgggg caacatgacc atgaatccgg
     1141 tgtcggccct cacgggtgcg acgctcgatc gggtgctgga tgatgagttg gtgcgcaatt
     1201 tctgctccgc ggccatgcgc gaggctgccg cggtcggcgc gcgcatcggc tgcgatgtgc
     1261 acgagtcccc ggaggaccgg cacgcggtca cgcgcagact cggtgccgcc aagacctcaa
     1321 tgctgcagga tgtcgaggcc ctgcgtccgc tcgagctcga tgcgctcgtc ggcgcagtac
     1381 gcgagatcgg agcgcgggtc ggagtggcga ccccggggat cgacgcgctt ttcggcctca
     1441 cccgactctt cggccaggtg cgcgggctgt acccggcgca ataaggctct ctaccctgct
     1501 gccgcagctc atctgccacg aaccatatca ggttgcaatc agactcagca tcgatgccgc
     1561 acgcgcgtgt tggttctcac gagctgtgat tgcgtagcgg actcgagcat gcaacggtga
     1621 accgtgcaac ggcagcattg tgcacagtga cctgcaccgc gctcgaatac agcgcattgt
     1681 aggccatcgt ctcatgtcgc agcgtcgaga cactttgcac tgagtgctgc aacgcggtgt
     1741 actgcgcccg ctccgtcgca ggttaagtca tccagccgct gcgcagtgcg cgcaacgtgg
     1801 gacccctgca gacgaagcgc acccgccgag taaggcgggc ggacgccagg gtggttctct
     1861 cgaagccacg agacagtggg gaagccattg agcaaccccg caggggtgct tttacctccg
     1921 caaggctgcc gtgcacatcg taggtgccgc ctcgacgacg cgtttcaact cagggaggcc
     1981 caatgaaccg aacatcgttc ctcagaccca cgctccacca gtttgtgcat cccgccgcgt
     2041 tagttatctt cctgaccgcc gcggctggcg tgtatccgtc gaacacctcc gacaaaccga
     2101 cgatctcggg cacccctgca accagtgcga gcgtcggaag cccttacgcc ttcgaaccct
     2161 cagcccatga tccgtacggc gcgccgctcg cgttttcgat aaacaacaag cccgcatggg
     2221 cgaatttcag cattgcgacc ggcagtcttt acggcgcgcc gacgagcgcg caggcgggga
     2281 cctatgccaa catcgagatc acggtgagca atgggaaggt ttctgcagcc ctgccgccct
     2341 tcagcatcac ggtcaagaat ggcgcgacct caacaaccac ggcgagcgca actcttagtt
     2401 ggacgccgcc gacggaaaat gctgatggca cggcgcttac cgatcttgcc ggggtgcata
     2461 tctactacgg caattccgca gcggatctga gccaggtcgt gcaagtctcg ggcacggatg
     2521 agagcagcta caccatcaag aatctggcag cggggacgtg gtacttcgct gcctcggctt
     2581 acaccaccac cggcgcgcaa agcgcgctat cgtcggtcgt gagcaagagc ataccttagg
     2641 cgtcgcgcgc cgcagtcaag ctgaatcggc gacatcccgg tcgcgccaca tgcctaaaag
     2701 tacgaccgcg aaactcgccg cgatcagaga ttccagaaaa agcgccggat acatgtagcg
     2761 catgtcgtgc tcgatgagca ggtaaaccag caggtagccg gcgatggcag cggtgaggca
     2821 gcggatgccg tatttatagc gcgaatgcca gagcaacaca gcgccggcaa tcgtcatgag
     2881 gctgatggca ccggcaatga ctcgttggta ccaacgcaca gactgtggca gcaccatgta
     2941 gcccagacgc tcggcgacca gccccagaaa ggcacgggga ttagccttta tccaggccaa
     3001 ggccgcgcgc tgcatctggt gattatattc gacctcaccc aggtcgcgta ctgacgccgc
     3061 agtggcgggg gaattgaaag gatggcgctg caacgtgcct ccggcagcgc cgttttctgt
     3121 ctccgtggcg cgggcgttcg ggccattgga catcgccagc tcgagcccaa aattgtcacg
     3181 catgaagaac catttgccga gcacgtgatg gttgcgcagg gtgtaaggca ggatggcaac
     3241 cgcgaaggcc agagtggcgc cggccaatag cgcgagcaga ccccttggtt tccagccaac
     3301 cagccgcgcg cccacgagtg tcacgaaggc cgccaccggc agcaccgtcg gggtgaaata
     3361 agcagcgagt ccggcggcaa agccgagtcc ggcgcccgtc gcaacacgcg cgcgtgcgcg
     3421 ctcgatggtg cgaatcacta cagtcacgac tgcgagaagc gcagctaccg taaacggtgt
     3481 ctcgtgttcg ccgctcagct caacccatgc gaagaacggt atcagccaga tcagagcggc
     3541 aatcgttccg gacgcaggcg gcagtttgag cgctcgagag atggggagga gcgccgcgat
     3601 attgcaggag ctgacgaccg cgagaatgat cgcgaggatg tgtagacagg catcatcgct
     3661 cggcgtgatc gagcgcaccg ccgccaccag caccgggtag gccggtgaga cgtgcgccgt
     3721 cggcccggtc gggatgacaa atggatcgcc caggtaaccg cgctgtgcga gggtgaatcc
     3781 tgccttgatg ttctcagtgt gagccgtggg cggacgcagt ggaacgagcg cgagcctcag
     3841 cacgaatgcc aaggcaaaca catgccacgt gcgcacctgt aacagcattc gtgacacggc
     3901 cactgccatc gaggagagcc ctccgcgagt ccttgccaga aacatgcgac gatccagaca
     3961 tgataacagc ctgccattga cacgatggat cggaaacaga ctcgatgcag agtaagtgga
     4021 atcagccgat acgcgccgca tccgtgcgga tggtgcggcc gctggtccac agatgccagg
     4081 ccgcccaaaa acctgtgggc gcgagcaaca acaacgccca gcgcaatgac tccgtgccgg
     4141 caccgaagga tgccgcgaac cagtcgctga gtgcgccgat gagctgcggg gcgagtaaca
     4201 ggttggcgat gttggcgagg aacagcagga tggcacaggt ggtggcgcgc atctgtgccg
     4261 ggaccacgtt ctgcagcagc ccgaggatcg ggccaatgta gaagtagacc gccggcacga
     4321 agatccacag cagcgcgacc gcggcacgct ccgacaccac ccagtagaga agcagcgacg
     4381 ggatagtggt gagcgccgta acccagccga gcagccgcgt cacgtaacgt gggtcgtccg
     4441 cggcgcggcg cgccatgagc cacccggcga agagcgtgct ggcggttcca gcaatgaggt
     4501 gcatcggccc aatgagttgg cccgcctgcc cgaccgtcag atgatgcgag cgttgcagga
     4561 atgccggcgt ccaccacatg agaccccaac tccagaaggt cgccacggaa ccgcccagaa
     4621 gcagatgcac ggcggagcgc tggtgcgcaa tgaaacgcag cgtcgccgtc aatgtgctgc
     4681 gcgtgcccgg caggtgcccg gcatcgagct ggccccggct cggctcgcgc acggtgagcc
     4741 atacgatcag cgcaagcact acgcccggaa tgccca
//
LOCUS       DATDMP010000182         3073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1361524, whole genome shotgun sequence.
ACCESSION   DATDMP010000182 DATDMP010000000
VERSION     DATDMP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361524"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..683)
                     /gene="dps"
                     /locus_tag="VI195_03065"
                     /gene_synonym="pexB"
     CDS             complement(159..683)
                     /gene="dps"
                     /locus_tag="VI195_03065"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEY6823393.1"
                     /translation="MSRTANQRPAKETPMFDTRNDLPANTRSKVIELLNARLADAIDL
                     GAQAKHAHWNVKGPSFIALHELFDKVAESVEEHVDALAERITALGGVAHGTLVAAARA
                     TSLKAYPDDISEGLAHVDALSAALADFGARVRGAIEGSGRLGNADTADLLTGISREND
                     KQLWFLEAHLHAKR"
     gene            complement(785..1747)
                     /locus_tag="VI195_03070"
     CDS             complement(785..1747)
                     /locus_tag="VI195_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823394.1"
                     /translation="MNATLDSAGEAPRSAAPASLAPAGVLLMLVRRELWENRALWIAP
                     LAVAAVLALGALFGHVGWDVDRERNMHGLDGVLGPAQQMALATIVQWGLSVPLYLVMV
                     CCVAYYLTDCLYAERKDRSILFWKSLPVSDGYTVLSKLLVAVVVVPVAVFVLALVFHL
                     LFSVIVALRVALGSVPHVLTWDTREWLRTEAAMLSTLVLAMLWYAPAAAYLLLVSAWA
                     RRSPLLWASLPLLLAPLLERIALGTHYIGGFIRYRTFGIWGTLALSHGHIVGHFDDLR
                     PVGTLLDTLHWGAAFTDADLWLGVLAAAALAYAATRIRRYRDDT"
     gene            complement(1744..2604)
                     /locus_tag="VI195_03075"
     CDS             complement(1744..2604)
                     /locus_tag="VI195_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6823395.1"
                     /translation="MTAVIEARGLDKSYGTTRALDQVSFSVAPGRIVGLIGPNGAGKT
                     TALKAILGLTSFAGELRVLGLDPRSQRDTLMREVCFMADVAVLPRWLRVAQALDFVAG
                     VHPRFDRAIAERFLGRTDIRRSSRVGQLSKGMVTQLHLALILAIDARLLVLDEPTLGL
                     DLLYRRQFYDTLLNDYFDKQRTILLTTHQVEEVEQLFTDVIFINRGKVALSASVEDLG
                     ARYQQLTLSAERASQARALRPFYEREVFGRVAMFFEGRSPAELASLGELRTPTIADLF
                     VAKMQGSAGT"
     gene            complement(2588..3004)
                     /locus_tag="VI195_03080"
     CDS             complement(2588..3004)
                     /locus_tag="VI195_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY6823396.1"
                     /translation="MDPQWDNSQPIYRQLRDRVVAMILEGVLKEGDPLPSVRNVAADF
                     RLNPLTVLKGYQQLVDEQLVEKRRGRGRFVSAGARKALMKDERQRFLEDEWPRVYATI
                     QRLGLSSDELLRAGAAAPATPWPASGNKGEQDDGGH"
BASE COUNT          505 a         1041 c         1069 g          458 t
ORIGIN      
        1 gtacatttca ctggttgatg tcccttcctg ggcggcttac accaccatca cgccgagcac
       61 gccgaacaac gggagctata ccgtcagctc gagctccagg ccctcgtcgg tgtggcggac
      121 ctcgaacggc atgggcgccg ctgaaagccc agcgccgttc agcgcttggc gtgcaggtgc
      181 gcctcgagga accagagctg cttgtcgttc tcgcgcgaga tgcccgtgag gaggtccgcg
      241 gtgtcggcat tgccgagccg acccgagccc tcgatcgctc cgcgcacccg ggccccgaag
      301 tcggcgagcg ccgccgacag cgcatcgacg tgtgccagcc cctcggagat atcgtccggg
      361 taggccttga ggctggtggc gcgcgccgcc gcgacgagcg taccgtgggc gacgccgccg
      421 agcgcggtga tgcgctcggc gagcgcatcg acgtgctcct cgacactctc ggcgaccttg
      481 tcgaacagct cgtgcagcgc aatgaagctc gggcccttga cgttccagtg ggcgtgcttg
      541 gcctgtgcgc cgagatcgat cgcgtccgcg aggcgcgcgt tgagcagctc gatcaccttg
      601 ctgcgggtgt tggccggcag atcgttcctg gtatcgaaca tgggggtctc cttcgctggc
      661 cgttggttcg cggtacggct catttaagca tcggcgcgcg ccggcgcgcc aatcgcctgt
      721 gcggatcgcg atgataagcg cgatctatcg ccgcagcgag aaaatcgcta tcccgccgct
      781 gtgctcaggt atcgtcccgg taacggcgga tgcgggtcgc cgcataggcg agcgccgcag
      841 cggcgagcac gcccagccac agatcggcgt cggtaaaggc cgcgccccag tgcagcgtgt
      901 cgagcagcgt gccgaccggc cgcaggtcgt cgaaatgacc gacgatgtgc ccgtgcgaga
      961 gcgcgagcgt gccccagatg ccgaaagtgc ggtagcggat gaagccgccg atgtagtggg
     1021 ttccgagagc gatgcgctcg aggagcggtg ccagcagcag cggcaggctg gcccagagca
     1081 gcgggctgcg ccgcgcccag gccgacacca gcagcaggta tgcggcggcc ggcgcgtacc
     1141 acagcatggc gagcaccagg gtggagagca tcgcggcctc ggtccgcagc cactcgcgcg
     1201 tgtcccaggt gagcacgtgc ggcacgctgc cgagcgccac gcgcagcgcc acgatcacgc
     1261 tgaacagcag gtgaaagacc agcgccagca cgaacaccgc caccggcacc accaccaccg
     1321 ccaccagcag cttcgacagc acggtgtagc cgtcggacac cggcagcgac ttccagaaga
     1381 gaatgctgcg gtccttgcgc tcggcgtaca ggcaatcggt gaggtagtag gcgacgcagc
     1441 agaccatcac caggtacagc ggcaccgaca ggccccactg cactatggtg gcgagcgcca
     1501 tctgctgcgc cggccccagc acgccgtcga gtccgtgcat gttccgctcg cggtccacgt
     1561 cccagccgac gtgaccgaag agcgcgccga gcgccagcac cgcggcgacc gcgagcggtg
     1621 cgatccacag tgcgcggttt tcccacagct cgcggcgcac cagcatcagg agcacgccgg
     1681 cgggagcgag cgaggcaggg gcggcgctgc gcggcgcctc gcccgcggag tcgagcgtgg
     1741 cgttcatgtg ccggcactcc cctgcatctt ggcgacgaac aggtcggcga tggtcggcgt
     1801 gcgcagctcc ccgaggctcg cgagctcggc gggcgagcgc ccctcgaaga acatcgccac
     1861 ccggccgaag acctcgcgct cgtagaaggg ccgcagcgca cgcgcctgcg aggcgcgttc
     1921 cgcgctgagc gtcagctgct ggtagcgggc gccgaggtcc tcgaccgagg cgctgagcgc
     1981 gaccttgccg cggttgatga aaatcacgtc ggtgaacagc tgctcgacct cctccacctg
     2041 gtgagtggtg agcaggatgg tgcgctgctt gtcgaagtag tcgttgagca gcgtatcgta
     2101 gaactggcgc cggtacagca ggtcgagccc gagcgtcggc tcgtcgagca ccagcagtcg
     2161 cgcgtcgatc gccagaatca gggcgagatg cagctgcgtc accatgccct tggagagctg
     2221 gccgacgcgg ctcgagcgtc gtatgtcggt cctgccgagg aagcgctcgg cgatggcgcg
     2281 gtcgaaacgc gggtgtaccc ccgcgacgaa gtcgagcgcc tgggcgaccc gcagccagcg
     2341 cggcagcacg gccacgtccg ccatgaagca cacctcgcgc atcagcgtgt cgcgctgcga
     2401 gcgcggatcg agcccgagca cgcgcagctc gccggcgaag gaggtgagtc cgaggatcgc
     2461 cttgagcgcg gtggtcttgc ccgcgccgtt cgggccgatg agcccgacga tccggcccgg
     2521 cgcgacgctg aagctgacct gatcgagggc gcgcgtcgtg ccgtaggatt tgtcgagtcc
     2581 gcgtgcttca atgaccgccg tcatcttgct cacccttgtt gcccgaagcc ggccacggcg
     2641 tggcgggcgc tgctgccccg gcgcgcagca gctcgtcgct cgagaggccg aggcgctgga
     2701 tggtcgcgta gaccctgggc cattcgtcct cgaggaagcg ctgccgttcg tccttcatca
     2761 gcgccttgcg tgcgccggcg ctgacgaacc tgccgcggcc gcggcgcttc tcgaccagct
     2821 gctcgtcgac cagctgctgg tagcccttga gcaccgtcaa cggattgagg cggaagtccg
     2881 ccgcgacatt gcgcaccgag ggcagtggat ccccctcctt cagcacgccc tcgaggatca
     2941 tggcaaccac ccgatcccgc agctggcggt aaatgggctg gctgttgtcc cattgcggat
     3001 ccatgcgtgc tcgtcgaaag ggaggaccgt gacgggagat tagcggacta atctaccaaa
     3061 acggccggct gcg
//
LOCUS       DATDMP010000183         5188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1695056, whole genome shotgun sequence.
ACCESSION   DATDMP010000183 DATDMP010000000
VERSION     DATDMP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695056"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1179)
                     /locus_tag="VI195_03085"
     CDS             complement(73..1179)
                     /locus_tag="VI195_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823397.1"
                     /translation="MSSRFQRFLLPGFAFKAVVIGGGYATGRELAEFFLPSGPWGGIA
                     AMVLATFIWSGVCVATFLFARATGARDYQSFVRALLGPGWLVFETAYVLFVILILAVF
                     GAAAGAIGAATLGLPTLAGTLALVIAIALFTAFGNTSVERLFKYVSFLLYGVYVLFVI
                     MALTHFGERIPQGFASNAPTDGWVLGGITYASYNIVGAIVILPVLRHLTSDRDAVIAG
                     LIAGPLAMLPALMFFSCMIAFYPQIASETLPSDFLLQQMHAPWFHLLFQLMIFAALLE
                     SGTGAVHAINERIDHAWRRHHAAPLAHRARLGIALATLTGCMLLAQRFGLVALIAKGY
                     RGLAVIFLLIYVLPLLTLGVSRLWQRAALRQGVS"
     gene            complement(1189..2235)
                     /locus_tag="VI195_03090"
     CDS             complement(1189..2235)
                     /locus_tag="VI195_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEY6823398.1"
                     /translation="MTALKLTVAIERLQFSAPFRIAGFLFEHQDAVVVTLEDGQHRGR
                     GEACGVYYLNDNAEQMVAALESRRAAIESGIDRASLQALLPAGGARNALDCALWELDA
                     RRTGKAVWELAGLDPPQPLVTTFTLGADEPAVMAEGARRWADAQALKVKLTGDRELDI
                     ARVKAVRAARPDVWLGVDANQAYPVSGLGRLIDALLEANITQLEQPVARGQEAGLENF
                     DSPIPLVADESALGLADLPGLVGRFNLVNVKLDKCGGLTEGLAMAREARRLGLGVMVG
                     NMMGSSLAMAPSFIVGQLCDVVDLDGPTFLAHDRTPAVTYSKGMIWVDEAVWGRVPLS
                     SAGGSGAAARAGRR"
     gene            complement(2265..2849)
                     /locus_tag="VI195_03095"
     CDS             complement(2265..2849)
                     /locus_tag="VI195_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823399.1"
                     /translation="MPALLYGFFACYAVTGKCDLRIDSKGADIPVFASMEDCQRFGSG
                     SAQQQPDKQGKWTVDQGHYYQCFGLTPVAMAASAPAEASRPVYKTTAETLQRDFQTNA
                     EALTRKIGGAVIEITGTVEAAGIADGAALQLAGDSWDVTAWLTPEGASAARGTPKHAR
                     ITLRCDRVGMLVAASGRRPAVVEVRDCKPVGSGG"
     gene            3054..3869
                     /locus_tag="VI195_03100"
     CDS             3054..3869
                     /locus_tag="VI195_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6823400.1"
                     /translation="MKHHRITGAGNTSLHVVETGATTGRPILFIHGLSQCWLQWTRQL
                     HSNLAEDFRLVAVDLRGHGLSEKPDDAYGDARLWADDLRSVIQGLKLDRPILCGWSYG
                     PLVMLDYLRHYGEDDIGGMQFVGGVTKLGSEAAASVLTPEFLNLVPGFFSNDVNESVR
                     SLGGLLRLCFSEQPQAQDYFLMLGWSLSVPPHVRQGLLARSIDNEDLLPKLRKPVLIT
                     HGAADAVVKPDVIDQQMRRISTARIDLMSTGHGCFWDDAERYNRQLREFASSL"
     gene            3969..4997
                     /locus_tag="VI195_03105"
     CDS             3969..4997
                     /locus_tag="VI195_03105"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEY6823401.1"
                     /translation="MIDIRSDTVTQPTREMLAAMLQAQVGDDVFGEDPTVNALQEKAA
                     ALFGKEAALFCPSGTMSNQIGIKIHTQPGDEVICDELAHVYLNEGGGIAANSGASVRL
                     LRGHRGRFTAEQVRENINRRDDSHVPWTRVVAIENTVNKGGGCCWDLREIERIRAVCR
                     ENDIAMHLDGARLFNALVARNESPRDHGRLFDTLSVCLSKGLGAPAGSLLLGSRQLLA
                     KAHRQRKVLGGGMRQAGYLAAAGLFALEHNVERLRDDHAKAKVLETELRALPYVAEVL
                     PAETNIVIFTLAEGIGTEGFLQQLQHQRVRASSIGPRSVRFVFHLDVSDAQLEELLHA
                     LRRVRPPA"
BASE COUNT          980 a         1699 c         1653 g          856 t
ORIGIN      
        1 cgttggtgcg cacttccgtg gcgcgcgcgc atgagcataa gtcaggtggc gaacgcggtg
       61 ctgcgaacga tctcatgaca ctccctggcg gagagctgca cgctgccaaa gccggctcac
      121 accgagcgtc aacagcggca gcacatagat gagcaggaaa atcacggcga gcccgcgata
      181 gcccttggcg atcagcgcca cgagcccgaa cctctgcgcg agcagcatgc aaccggtcag
      241 tgtggcgagc gcaatgccga gacgcgcgcg gtgcgcgagc ggtgccgcat gatggcggcg
      301 ccaggcatgg tcgatgcgct cgttgatggc gtgcaccgcg cccgtgccgc tctcgagcag
      361 cgccgcgaaa atcatcagct gaaagagcag atggaaccag ggcgcatgca tctgctgcag
      421 aagaaaatcc gagggcagcg tctcggaagc gatctgtggg tagaaggcga tcatgcagct
      481 gaagaacatg agagccggca gcatcgcgag cggtcccgca atgagcccgg caatcacggc
      541 atcccggtcg ctcgtcaggt gacgcagcac cggcagaatg acgatcgcac cgacgatgtt
      601 gtaactggcg taggtgattc cgcccagcac ccagccatcg gtcggtgcgt tgctggcgaa
      661 gccctgcgga atgcgctccc cgaagtgcgt cagcgccatg atgacgaaca gcacgtaaac
      721 gccgtaaagc agaaacgaca cgtatttgaa gagccgctcg accgaggtgt taccgaatgc
      781 tgtgaagagc gcaatggcga tgaccagtgc cagggtgccg gcgagcgtcg gcagtccaag
      841 cgtcgcggcc ccgatggcgc ccgccgcggc gccgaagacc gcgagtatca ggatgacgaa
      901 gagcacatag gcggtttcga acaccagcca gcccggaccg agcagcgcac gcacgaagct
      961 ctggtagtcg cgtgcaccgg tcgcgcgcgc aaagagaaaa gtcgccacgc acacgccgct
     1021 ccagatgaag gtggcgagca ccatcgccgc aatgccgccc caggggccgc tcggcaggaa
     1081 gaattcggcg agctcgcggc cggtggcata accgccgcca atgacgaccg ccttgaaggc
     1141 aaagccgggg agcagaaagc gctgaaaccg tgatgacacc gcctggtcct agcggcggcc
     1201 cgcgcgcgct gccgcgcccg agcccccagc ggaggagagc gggacgcgcc cccagaccgc
     1261 ctcatcgacc cagatcatcc ccttcgaata ggtcacggcc ggagtgcgat cgtgcgcgag
     1321 aaaggtcggt ccatcgagat ccacgacgtc gcacagctga cccacgatga acgaaggtgc
     1381 catggcgagg ctcgagccca tcatgttgcc caccatcacg ccaagaccga gccgccgcgc
     1441 ttcccgcgcc atggccaagc cctcggtgag gccaccgcat ttatcgagct tgacgttgac
     1501 caagttgaaa cggccgacga ggcccggcag atcggccagt cccagggcac tctcatccgc
     1561 gacgagtggg atcggcgaat cgaagttctc gagccctgct tcctggccgc gcgcgaccgg
     1621 ctgctcgagt tgcgtgatgt tggcctcgag gagagcgtcg atcagccgcc cgagaccgga
     1681 cacggggtac gcctgattgg cgtcgacacc gagccagacg tccggccgcg ccgcgcgcac
     1741 cgccttcacc cgggcgatgt cgagctcgcg atctcccgtc agcttgactt tgagcgcctg
     1801 agcatcggcc cagcgacgcg ctccctcggc catgaccgcg ggctcatccg ccccgagcgt
     1861 gaaggtcgtt accagcggct gaggcggatc gaggccggca agctcccaca ccgccttacc
     1921 cgtgcgccgc gcatcgagct cccacagcgc gcaatccagc gcattgcggg caccgcctgc
     1981 ggggagcagc gcctgcagag aggcccggtc gatgcccgat tcgatcgcgg cgcggcgact
     2041 ttcgagcgcc gcgaccatct gctcagcgtt gtcattgaga taatagactc cacaggcttc
     2101 accgcgaccg cggtgctggc catcctccag tgttacgacg accgcatcct gatgctcgaa
     2161 caggaatccg gcgatgcgaa agggagccga gaactgcagc cgctcgatgg cgaccgttag
     2221 cttgagtgcc gtcatcagaa ggaaaaccgg ggcttcaccc gtcgctaccc gccggagccg
     2281 accggcttgc aatcacggac ctcgaccacc gccgggcgtc tgcccgatgc cgctaccagc
     2341 atcccgacgc ggtcgcagcg cagagtgatc cgcgcgtgtt tgggggtacc ccgcgcagcg
     2401 gaggctccct ctggcgtaag ccatgccgtg acatcccacg aatcgccagc tagttgcagc
     2461 gcagcgccat ccgcaattcc ggcggcctcg acggttccgg tgatctcgat gaccgcaccg
     2521 ccgattttcc gcgtgagggc ctcggcatta gtttggaagt cgcgttgcag ggtctcggcc
     2581 gtcgtcttgt acacgggccg ggacgcctca gcgggcgctg acgccgccat cgcgacgggt
     2641 gtcaggccaa aacactggta atagtgaccc tgatcgaccg tccacttgcc ttgtttgtcc
     2701 ggctgctgct gcgcggagcc ggatccgaag cgctggcaat cctccatcga tgcgaacacc
     2761 gggatatcgg ctcccttgct atcgatgcgc aggtcacact tgcccgtcac cgcgtaacac
     2821 gcaaagaagc cgtacagcag agcaggcacg actcacctcc aggcttggta tttgtcttac
     2881 gcgccgtgtg gccgcgagca gcgggtagga agcctagcac gtctctgagc aatcgccggg
     2941 gctgtagcgc ctcgctcaga gagctcgttg cggcccgaga cggaccggcc tactgtatcg
     3001 ctacaccgca gctagatctg attggctcgt cgaccgatcc acgggagggt gtgatgaagc
     3061 atcatcggat aacgggcgcg ggcaacactt cgctgcacgt cgtcgagacc ggcgctacaa
     3121 cgggcaggcc gattctcttc attcacgggc tttcgcagtg ctggctgcag tggacccgac
     3181 agctgcattc gaacctggcc gaagatttcc ggctggtggc ggtggatctg cgcgggcacg
     3241 gcctatcgga gaagccagac gatgcgtacg gagacgcacg cctgtgggcc gatgacctgc
     3301 gatccgtgat ccaagggctg aagctcgatc gtcccatcct gtgcggttgg tcctatgggc
     3361 cgctcgtgat gctcgattac ctgcgccact acggcgagga cgacatcggc ggcatgcagt
     3421 tcgtaggcgg tgtcaccaag ctcggcagcg aagcggctgc gtcggtactg accccggagt
     3481 tcctcaatct cgtgccgggc ttcttctcga atgatgtcaa cgagagcgtt cgcagcctgg
     3541 gtggtctgct gcgattgtgc ttctccgagc agccacaagc gcaagactat tttctgatgc
     3601 tggggtggag cttgagcgtg ccgcctcatg tgcgtcaggg cctgctcgca cgctccatcg
     3661 acaacgagga ccttctgccc aaactgcgca aaccggtact gatcacccat ggcgccgcgg
     3721 acgcggtggt caagcctgac gtcatcgatc agcagatgcg ccgaatcagc acggcgagaa
     3781 tcgacctcat gtcgaccggc cacggctgct tctgggatga tgccgagcgt tataaccgcc
     3841 agctgcggga gttcgcgtcc tccctgtagg gttgcgcatc gaggctgccc cccgggagtg
     3901 attgactaac gccctgcagg ccgcagaatt cgtccatacc tcgcggcggg agctctgcta
     3961 tgccacccat gatcgatatc cggagcgata ccgttacgca gcctacccgg gagatgctcg
     4021 cggcaatgct gcaggcgcag gtgggcgatg acgtcttcgg cgaggacccg accgtcaatg
     4081 ctctgcagga gaaggcggcg gcgctctttg gaaaagaggc tgctctgttc tgtccgagcg
     4141 gcaccatgtc caaccagatc ggcatcaaga tacacacgca gcccggcgac gaagtgatct
     4201 gtgatgagct cgcgcacgtg tatctcaacg agggcggcgg tatcgcggcc aactccgggg
     4261 cgtccgtgcg cctgctccgc ggccaccggg gacgcttcac ggccgagcag gtgcgggaga
     4321 acatcaatcg ccgcgacgat tctcatgtgc cctggacccg tgtcgtcgcc atcgagaaca
     4381 cggtcaacaa gggtggaggt tgctgctggg acctgcggga gatcgagcgc atccgggccg
     4441 tttgccggga aaacgacatt gcgatgcacc tcgacggcgc gcggcttttc aatgcgctcg
     4501 tggcccgcaa cgagtcgccc cgcgaccatg gacgtctctt cgacaccctt tccgtatgcc
     4561 tctccaaagg acttggcgca ccggcgggct cactgctcct cgggtcacgg caacttctcg
     4621 ccaaagcgca tcgccagcgc aaagtactcg gaggcggcat gcggcaggcg ggatacctgg
     4681 ctgccgcggg gttattcgcc ctggagcaca atgtcgagcg tctgcgtgac gaccacgcaa
     4741 aggcgaaggt gctcgagacc gagctccggg ccctgcccta cgttgccgag gttctacccg
     4801 ccgagacgaa catcgtcatc ttcacactgg cagaggggat cggcaccgag ggcttcctgc
     4861 agcagctgca gcatcagcgc gtgcgggcat cgagcatcgg accgcgctcg gtgcgcttcg
     4921 tcttccacct ggacgtcagt gatgcgcagc tcgaagaact cctgcacgcg ttacgccgcg
     4981 tacggccacc cgcttgagag cgctccaccc tttcccgggc taccgttcgc tcttcagtaa
     5041 aatggcgccg atgagatccc tcctgggaat ggcggttttg tgcttgtgcc tggcctcagc
     5101 cgcatcggcc gcggacagca tgcgcgccgt cgtgttcatc gacggccact ttcagctcca
     5161 gtcggtcccc aaacctgagc cgcaggcc
//
LOCUS       DATDMP010000184         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_28922, whole genome shotgun sequence.
ACCESSION   DATDMP010000184 DATDMP010000000
VERSION     DATDMP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28922"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1833
                     /locus_tag="VI195_03110"
     CDS             112..1833
                     /locus_tag="VI195_03110"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY6823402.1"
                     /translation="MSGWVDSVRELTQPRAIHWCEGTEAEAHEITAQLLSRGELTALN
                     PEQFPDCYLYRSAPSDVARVEHLTYICTRSREDAGPNNHWMDPQQAHARLRELFRGCM
                     RERTLYVIPYCMGPLDSPLSRCGVEITDSPYVVLNMLIMTRAGRAALERIAREQRFVR
                     GLHSIGELDPERRFIMHFPEELAIESYGSGYGGNALLGKKCHALRIASWQARSEGWLA
                     EHMLIVGLQNPRGETHYLACAFPSACGKTNLAMLIPPASLPGWRVFTVGDDIAWLHPG
                     ADGRLWAINPESGYFGVVPGTNPQTNRNAYEMIRRDTLFTNVALTADNQPWWEGLPSG
                     TPVLDWQGRAYDPARGPAAHPNSRFTVSARRNPSYSPHAEDARGVPISALVFGGRRRE
                     VAPLVYEARDWQHGVLVGASLASETTAAAVGQVGVTRRDPMAMQPFCGYNFGDYWQHW
                     LEVGAQLPRPPRIYHVNWFRRNTSGKFLWPGYGENLRVLAWMLERCAGRAGANESAIG
                     RLPRPEDLELRGLELGPQALAALLAVDPALWRKEIAELRAYLGGYGARLPAALLAELR
                     TTEQRLG"
     gene            complement(1851..>2589)
                     /locus_tag="VI195_03115"
     CDS             complement(1851..>2589)
                     /locus_tag="VI195_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY6823403.1"
                     /translation="GDEVMATFPSADAALNAAAQMQQQISTHAQLKVEGQPVAIRIGC
                     HFGPVMLENRDVFGAAVHTANRMTSQAKAGQIVTTAAMVERLSPEWRAACRQIDIATL
                     KGQGSEVALYEVLWQTEDVTSMVPGIATESRPARIMRLRLRCDDRELLVDERHSSITI
                     GRAEDNDVVVKGNLISRLHARIEISRSKFVLIDQSTNGTFVQSSDGEESFVRRDSLQI
                     RGQGMIGLGRLPEQGSPQTIRFTCEEG"
BASE COUNT          412 a          892 c          845 g          440 t
ORIGIN      
        1 acagcacccg cgatataata agaagtttca accaagcgtc acggacaaga ggaacgcgcg
       61 atggccactg cagcagaccg gctgcgcccc ctgccccggc tgagccccgc cgtgagcggc
      121 tgggttgact cggtgcgcga gctcacccag ccgcgcgcca tccactggtg cgagggaacc
      181 gaggccgagg cgcacgagat cactgcccaa ctgctgtcgc gcggggagct gaccgcgctc
      241 aacccggagc agtttccgga ctgctacctg taccgttcgg cgccgagcga tgtggcgcgc
      301 gtcgagcacc tcacctacat ctgcacgcgc tcacgcgagg atgccggccc caacaaccac
      361 tggatggacc cgcagcaggc gcacgcgcgg ctgcgcgagc tgttccgcgg ctgcatgcgc
      421 gagcgcactc tgtacgtgat tccctactgc atggggccgc tcgattcgcc gctgtcgcgc
      481 tgcggcgtgg agatcaccga cagcccctat gtcgtgctca acatgttgat catgacgcgt
      541 gccgggcgcg cggcactcga gcgcatcgcg cgcgagcagc gcttcgtgcg cggactccat
      601 tcgatcggcg agctcgatcc cgagcggcgt ttcatcatgc actttcccga agagctcgcg
      661 atcgagagct atggttccgg atatggcggc aacgcgctgc tcggcaagaa atgccacgcg
      721 ctgcgcatcg ccagctggca ggcgcgcagc gagggctggc tcgccgagca catgctgatc
      781 gtcgggctgc agaatccgcg cggcgagacg cactaccttg cctgtgcctt tccctccgcg
      841 tgcggcaaga ccaacctcgc catgctgatt ccgccggcct cgctcccggg ctggcgggtg
      901 ttcaccgtcg gcgatgacat cgcctggctc catccgggtg cggatgggcg tctgtgggcc
      961 atcaatcccg agtccggtta cttcggcgtc gttcccggta ccaatccgca gaccaaccgc
     1021 aacgcctatg aaatgatccg ccgcgacacc ctgttcacca acgtcgcgct gaccgcggac
     1081 aaccagccct ggtgggaagg acttccgagc ggcacgccgg tgctcgactg gcagggccgt
     1141 gcgtacgatc cggcacgggg ccccgcggcg catccgaact cgcgcttcac cgtatctgcg
     1201 cgccgcaacc cgagctattc accacacgcc gaggacgcac gcggcgtgcc gatatccgcg
     1261 ctggtgttcg gcggacggcg gcgcgaggtg gcgccgctcg tctacgaggc acgcgactgg
     1321 cagcatggag tgctggtcgg cgcatcgctc gcttcggaga ccacggcggc ggccgtgggc
     1381 caggtcggtg tcacgcgccg tgacccgatg gccatgcagc cattctgcgg atacaacttc
     1441 ggcgattact ggcagcactg gctcgaggtc ggcgcgcagc tgccgcgccc gccgcgcatc
     1501 taccacgtca actggttccg tcgcaacacg agcggcaagt tcctctggcc gggctacggc
     1561 gaaaatctgc gtgtgctcgc ctggatgctc gagcgctgcg cgggacgcgc cggtgcgaat
     1621 gaatcggcga tcggccgcct gccacgtccc gaggatctgg agctgcgcgg gctcgaactc
     1681 ggaccacagg cactcgcggc acttctcgcg gtagaccccg ccctgtggcg caaggagatc
     1741 gccgagctgc gtgcctatct cggcggctac ggcgcgcgcc tgccggctgc gctgctggcc
     1801 gagttgcgca ccacggagca gcgcctcggc tgaacttgcc ggcctggctt tcagccttct
     1861 tcgcaggtga agcgaatggt ctgcggcgaa ccctgctcgg gcaggcgccc gaggccgatc
     1921 atgccctgac ctctgatctg caggctgtcg cggcgcacga aagactcctc gccgtccgag
     1981 ctctgcacga aggtaccgtt ggtgctctga tcgatgagca cgaacttgct gcggctaatc
     2041 tcgatgcgtg cgtgcagacg cgagatgaga ttgcctttga cgaccacgtc gttgtcctcg
     2101 gcgcggccga tggtgatgct cgaatgacgc tcatcgacca gcagctcgcg gtcatcgcag
     2161 cgtagtcgca ggcgcatgat acgcgccgga cgcgactcgg tggcgatgcc gggcaccatg
     2221 ctggtgacat cctcggtctg ccacagcact tcgtacagcg ccacctcact tccctgaccc
     2281 ttgagcgtcg cgatgtcgat ctgacggcac gcggcacgcc attccggtga cagccgctcg
     2341 accatcgctg cggtggtaac gatctggcca gccttcgcct ggctcgtcat gcggttggcg
     2401 gtatgtacgg ccgcgccgaa cacgtcgcgg ttctcgagca tcacgggtcc gaagtggcaa
     2461 ccgatgcgaa tcgcgactgg ctggccttcg accttcagct gcgcgtgggt ggagatctgc
     2521 tgctgcatct gcgccgcggc gttgagggcg gcatcggcgc tcgggaaggt cgccatcacc
     2581 tcatcgccc
//
LOCUS       DATDMP010000185         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2027886, whole genome shotgun sequence.
ACCESSION   DATDMP010000185 DATDMP010000000
VERSION     DATDMP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027886"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VI195_03120"
     CDS             complement(<1..627)
                     /locus_tag="VI195_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY6823404.1"
                     /translation="MSGPGNISALLALPDRFAGLHIWVVGDLMLDEYVTGAVERISPE
                     APVPVVRAQATEHRLGGAANVARQIATLGARVSLAGICGADPPGEELLQLCAQAGIDT
                     RAVLKLEGRHTTRKLRVVSHSQQLLRLDWEDIQPCAAAVTLQLLEKLAAERPPDAIIL
                     SDYAKGVLTPHTIAAVARARGTVPLVVDPKHRDFARYRGATTLTPNLHE"
     gene            complement(624..1199)
                     /locus_tag="VI195_03125"
     CDS             complement(624..1199)
                     /locus_tag="VI195_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY6823405.1"
                     /translation="MTIAEIFADHAAVAARAALELPPALERIIPMLHACLRGGGKILA
                     CGNGGSATDAQHLVAELVGRFREERPALPAIALSANTATLTALGNDYGFERVFARQVE
                     ALARRGDVLIAISTSGNSANVLEAAQAARSRGCSVVALTGAHGGQLAAHSDLILKAPS
                     HTVARIQEVHALCIHVICESLDELLRAQETR"
     gene            complement(1186..1746)
                     /gene="gmhB"
                     /locus_tag="VI195_03130"
     CDS             complement(1186..1746)
                     /gene="gmhB"
                     /locus_tag="VI195_03130"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY6823406.1"
                     /translation="MSIRHVILDRDGVLNYDAPAGSYVREPAEFRWLPGALEGLALLR
                     RAGLRLSVATNQAGVGRGVMSPEQLAAVHARMQAEAAAHGGALDAVLYCPHAPEEQCS
                     CRKPAPGLIAAAVASSGIPAGESIVVGDDQRDLEAARRAGVAAALVRTGKGRRTEELL
                     EDAAVRVYDDLPQLARAIVGMSHDDS"
     gene            complement(1769..>2071)
                     /locus_tag="VI195_03135"
     CDS             complement(1769..>2071)
                     /locus_tag="VI195_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823407.1"
                     /translation="CVEVVQWLLAHREVNGLYNLGTGRARSFADLAQALFAALRRPAR
                     IEYIDTPPAVRAHYQYFTEARMQRLREVGYARPFTSLEDGVADYVQRYLAQADPYL"
BASE COUNT          313 a          733 c          720 g          305 t
ORIGIN      
        1 ctcgtgcagg ttgggcgtca gcgtcgtcgc gccgcggtag cgcgcgaagt cacggtgctt
       61 cgggtcgacg acgagcggaa ccgtgccgcg cgcacgcgcg acggcggcga tagtgtgcgg
      121 ggtcaacaca cccttggcat agtcgctgag gatgatcgcg tccggcggcc gctccgcggc
      181 gagcttctcg agcagctgca gcgtgacagc ggccgcgcac ggctggatgt cctcccagtc
      241 gagtcgcagc agctgctggc tgtggctcac tacgcgcagc ttgcgcgtcg tatgccggcc
      301 ctcgagcttc aataccgcgc gcgtatcgat gcctgcctgt gcgcacagct gcagtaactc
      361 ctccccggga ggatcggccc cgcagatgcc cgcgaggctg acgcgtgcgc cgagcgtcgc
      421 gatctgccgt gccacgttgg cggcgccgcc gaggcggtgc tcggtcgcct gcgcccgcac
      481 caccggcacc ggggcctcgg gcgagatccg ctccacggcc cccgtgacgt actcatcgag
      541 catcaggtcg ccgacgaccc agatgtggag cccggcgaac cgatccggca acgcgagcag
      601 cgcggagatg ttgcctggcc cgctcaccgg gtctcctgcg cgcggaggag ctcatcgagc
      661 gattcacaga tcacgtggat acagagcgcg tgcacttcct ggatgcgcgc aacggtgtgc
      721 gacggcgcct tgaggatgag atcgctgtgt gcggcgagct gaccgccgtg cgcgccggtc
      781 agtgcgacca cgctgcaacc gcggctgcgc gccgcctgcg ccgcctcaag cacattcgcc
      841 gagttaccgc tggtactgat ggcgatgagc acatcgccgc ggcgtgcgag cgcttccacc
      901 tggcgcgcaa acacgcgctc gaacccgtag tcgttgccga gcgcagtcaa agttgccgtg
      961 ttggcgctga gcgcgatcgc cggcagcgcc ggccgctcct cgcggaagcg cccgaccagc
     1021 tcggccacga ggtgctgcgc gtcggtcgcg ctgccaccgt tgccacaggc aagaatcttg
     1081 ccgccgccgc gcaggcaggc gtgcagcatg gggatgatgc gctcgagcgc aggcggcaac
     1141 tcgagcgccg cgcgtgcagc aaccgccgcg tgatcggcga agatctcagc tatcgtcatg
     1201 actcattccc acgatggcgc gtgccagctg cggcagatcg tcgtacacgc ggaccgcggc
     1261 gtcttccagg agctcctcgg tgcggcggcc tttgccggtg cgcaccagcg ctgccgccac
     1321 cccggcacgc cgcgccgctt cgaggtcgcg ctgatcgtcg ccgacgacga tgctctcgcc
     1381 tgccgggata cccgagcttg ccacggccgc tgcaatcagc ccgggcgccg gtttgcggca
     1441 cgagcactgc tcctctgggg catgcggaca gtagagtacc gcatcgagcg caccgccatg
     1501 ggccgcggct tcggcctgca tgcgcgcgtg cacggcggca agctgctcgg gactcatgac
     1561 gccccgcccc acgcccgcct gattggtggc caccgagagc cgcaaccccg cgcggcgcag
     1621 cagcgccagg ccctcgagcg ccccgggcag ccagcggaac tcggccggct cacgcacgta
     1681 gctgccggcc ggcgcgtcgt aattcaacac gccgtcgcgg tcgagaatca cgtggcggat
     1741 actcatggcg gcagccgggc acgggtggct agaggtacgg gtcggcttgc gcgaggtagc
     1801 gctgcacgta atccgccacc ccgtcctcga gtgaggtgaa cgggcgggcg tatccgacct
     1861 cgcgcaagcg ctgcatgcgc gcctcggtga agtactggta atgcgcgcgc actgccggtg
     1921 gtgtatcgat gtactcgatg cgcgcgggcc gtcgcagcgc ggcaaagagc gcctgggcca
     1981 gatcggcgaa gctgcgcgcg cggccggtgc cgagattgta caggccattc acctcgcgat
     2041 gagcgagcag ccactgcacc acctcgacac a
//
LOCUS       DATDMP010000186         2888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_917974, whole genome shotgun sequence.
ACCESSION   DATDMP010000186 DATDMP010000000
VERSION     DATDMP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917974"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1547)
                     /gene="tagF"
                     /locus_tag="VI195_03140"
     CDS             complement(63..1547)
                     /gene="tagF"
                     /locus_tag="VI195_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEY6823408.1"
                     /translation="MEVGLYGKLPSHGDFLRRRIADEIVSCWDGWLQSAISASRRILG
                     ERWLEIYLTSPAWRFSCEAGACGSTPLAGVIVPSVDRVGRYFPLTLMWQIPDELTPLS
                     VAMLCNSWFEAAERHVIETLAQNEIHFEEFDARVVALGEQLDAMCATQRVVLDPHDAQ
                     TVTETRAMWQVPLGDPSRLSELFNQLLHHRLRVSCAPLMLWWTEGSAMVAPCALLTPG
                     LPPEDHFAALLGGSWSECGWRTVGAKVRSEAFVDTLVPDPIVEFRSAGLSDQGRVRES
                     NQDAFLERPEVGLWAVADGMGGHAGGDVASRMVCDALADLSPADTLQNMVERVRDRLG
                     EVNTQVRGASQPTSGHTGSGSTVAVLLTRNAQCSILWAGDSRVYRMRDQRLEQLTRDH
                     SYALEIANGTMPAESEEAIADYSITRAVGGEETLELELRRDHVRKGDRFLLCSDGLTH
                     ELDDAHIEACLQGGDPTESARKVLQAALEAGGHDNVTVIVIDAT"
     gene            complement(1584..>2888)
                     /gene="tssM"
                     /locus_tag="VI195_03145"
     CDS             complement(1584..>2888)
                     /gene="tssM"
                     /locus_tag="VI195_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEY6823409.1"
                     /translation="RMTGQVIDIYEQDYIRAWDAILNDITLVPSKSVAQATETMAIIA
                     GPTSPLKGFLLVVESNTNLTKPKPGAGKVESAATEAVRATQEQLRKLLGGGAQDSGSA
                     AARPGTRVATHFARINQLVAAAPGGGPAAIDGVLAQIGQIQKQLNSVGSGLGETNALE
                     AVVHSGSGDALKSLSQQASTLPPPVDGLIAAIAKSSETLTTGQARGELNNRYEQQVLR
                     DCREIVNGRFPFQPQSAVDVPLADFGRLFGYGGLFDTFFKDNLAAVVDTTHTPWTWRS
                     GATGSIAGSASMLREFEVAQQIRDMFFRPGGQLPQVQFNVAPSYLGANDTRFVLELDG
                     QSVEYRHGPERNSPVTWPGPAPGAAAISFEDRTGVHPNLSYQGAWAWFRLLDAAAVQP
                     ESDVRYRATFKAGGSEARVTIEAASIRNPFAKSALRQFRCAG"
BASE COUNT          486 a          987 c          904 g          511 t
ORIGIN      
        1 cgactgatca gtcggtcgag aagatcatcg agtgcgcgac cgatggccat ccgtgtcctt
       61 ctttaggtgg cgtcgatcac gatgaccgtc acgttgtcat gaccgcccgc ctcgagcgct
      121 gcctgcagca ctttgcgcgc actttccgtg ggatctccgc cctgcaggca tgcctcgatg
      181 tgcgcatcgt cgagctcatg ggtgaggcca tcggagcaca acaggaaccg atcacccttt
      241 ctcacatgat cgcggcgcaa ttcgagctcc agcgtctcct cccctccgac cgcacgcgtg
      301 atcgagtaat cggcgatcgc ctcttccgac tctgcgggca tcgtgccgtt cgcaatctcc
      361 aacgcatagc tgtgatcacg cgtcagctgt tccagcctct gatcgcgcat ccggtagacg
      421 cggctgtcac ccgcccacag gatcgagcac tgggcgttgc gggtgagcag cactgcgacc
      481 gtgctgccgc tcccggtgtg accggaggta ggctgggaag cgccgcgcac ctgggtgttc
      541 acttcgccaa ggcgatcgcg cacacgctca accatgttct gcaaggtatc ggcgggagac
      601 aggtccgcga gggcatcaca caccatgcgg ctcgcgacgt ctccgccggc atggcctccc
      661 atgccgtcgg cgaccgccca caggcccacc tcggggcgct cgaggaacgc gtcctggttg
      721 ctctcgcgca cgcgtccctg atcgctcagc cccgccgagc gaaactccac gatggggtcc
      781 gggacgagtg tgtcgacgaa tgcttcggag cgcacctttg cgccgacggt tcgccatccg
      841 cactcagacc aggatccgcc gagaagcgct gcgaaatgat cctctggtgg cagacccggt
      901 gtcagtagcg cgcacggcgc caccatcgcg gagccctccg tccaccagag catcagcggc
      961 gcacacgaaa ctcgcaaccg gtgatgcagc aactggttga aaagttcgct gaggcgtgac
     1021 gggtcgccga gcgggacttg ccacatcgca cgcgtctccg tgaccgtctg cgcgtcgtgc
     1081 gggtcgagaa ctacgcgctg cgtcgcgcac atggcgtcga gctgctcccc gagcgcgacc
     1141 acgcgcgcgt cgaattcttc gaagtggatc tcgttctgcg cgagcgtctc gatcacgtgc
     1201 cgctcggcgg cctcgaacca gctgttgcac agcatcgcga ccgacagcgg tgtcagctca
     1261 tccggaatct gccacatcag cgtcagcggg aagtagcgcc ccacccggtc tacgctcggc
     1321 acgatcacgc ccgccaacgg tgtcgagccg caagcccccg cttcgcagct gaaccgccat
     1381 gccgggctcg tcaaatagat ctcgagccag cgctccccga gaatgcgccg gctcgcgctg
     1441 atggcgctct gcagccatcc gtcccagcag ctgacgatct catcggcgat gcgccggcgg
     1501 agaaagtcac cgtggctcgg cagcttgccg taaaggccga cctccaccct gatccccttt
     1561 gatcgcgcga cttctgccgc gattcaaccg gcacagcgga attgccgcag cgccgacttc
     1621 gcgaacggat tacgaatgct cgccgcctcg atggtcaccc gcgcctcgct cccgccggcc
     1681 ttgaaggtgg cgcgatagcg cacgtccgat tccggctgca cggctgcggc atcgagcaat
     1741 ctgaaccagg cccacgcacc ctggtaggaa agattcgggt gcactccggt gcggtcctcg
     1801 aaggagatcg cggcagcccc gggcgctgga ccgggccagg tcacgggtga gttgcgctcg
     1861 ggcccgtgcc ggtactcaac gctctgtccg tcgagctcca gcacgaagcg ggtgtcattg
     1921 gcgccgagat acgacggcgc aacattgaac tgaacctggg gtagctgacc gcccggccgg
     1981 aagaacatgt cgcggatttg ctgcgcgacc tcgaattcgc gcagcatgga ggccgagccg
     2041 gcgatggagc ccgtagcacc gctgcgccac gtccacggcg tgtgcgtggt atcgacgacc
     2101 gctgcaaggt tgtccttgaa gaaggtgtcg aacaggccgc cataaccgaa aagacgcccg
     2161 aagtcagcga gcggcacatc gaccgcgctc tggggttgga acggaaagcg gccattgacg
     2221 atttcgcggc agtcgcgcag cacctgctgc tcgtagcggt tattgagctc gccgcgcgcc
     2281 tggcccgtcg tgagtgtctc gctgctcttc gcgatggcgg cgatcagccc gtcgacgggc
     2341 ggcggcagcg tgctcgcctg ctgcgacaac gatttgagcg cgtccccgct gccggaatgt
     2401 acgaccgctt cgagcgcgtt cgtctctccg agacccgaac cgaccgagtt gagctgcttc
     2461 tggatctgac cgatctgcgc cagcacccca tcgatcgccg cggggcctcc cccgggagcg
     2521 gccgccacca gttggttgat gcgcgcgaag tgcgtggcga cacgtgtacc cggcctggcg
     2581 gcggcactac cggaatcctg cgccccccca ccgagcagct tccgcagctg ttcctgggtc
     2641 gcccggaccg cttcggtggc ggcgctctcc accttgcctg cgccgggctt tggtttcgtc
     2701 aggttggtgt tcgactcgac gaccagcagg aaccctttga ggggtgaggt cggaccggca
     2761 atgatcgcca tcgtctcggt cgcctgggct acgcttttag atggcaccag tgtgatgtcg
     2821 ttcaaaatcg catcccaggc gcggatgtaa tcctgctcgt aaatatcgat gacttggccg
     2881 gtcatgcg
//
LOCUS       DATDMP010000187         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1472982, whole genome shotgun sequence.
ACCESSION   DATDMP010000187 DATDMP010000000
VERSION     DATDMP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472982"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VI195_03150"
     CDS             complement(<1..376)
                     /locus_tag="VI195_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823410.1"
                     /translation="MRNLLRALACTLLLAALPPAAEAGVFVGVSVNFAPPPLPVYVQP
                     PCPAPGFVWTPGYWAWGPDGYFWVPGTWLLAPVGLLWTPGYWGWADGVYLWHAGYWGP
                     QVGFYGGINYGFGYTGVGFEGGY"
     gene            complement(537..1154)
                     /locus_tag="VI195_03155"
     CDS             complement(537..1154)
                     /locus_tag="VI195_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY6823411.1"
                     /translation="MSVQALFPTLVYTQRLRGASWRALNARLLRECRQLRAEDGAGRR
                     WSARNYPGGYTSYGSASRMHRQSPTFAALARELDRRVLRFAAALEFDLDGRALAMTDC
                     WVNIMGRGAAHGLHLHPLSTISGSYYVAVPAGSPGLKLEDPRLERYMAAPPRRTRAAR
                     ARRPWVVITARSGQLVLFESWLRHEVPPQAGAGERVSISFNYGWF"
     gene            1169..2779
                     /locus_tag="VI195_03160"
     CDS             1169..2779
                     /locus_tag="VI195_03160"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY6823412.1"
                     /translation="MRAVALVVYDLDTMAVSTVPARAPGHAAPESGSLPSVPHWIGGR
                     AVAGAGALLDVFNPATGEVARRVALAGEAEVHAAVQSAQAAWPAWADTPPVRRARVLA
                     RFIALLERERDALAALITAEHGKVFADAQGEVTRGIEVVEFACGIPQLLKGDFTDQVS
                     TGIDNWTLRQPLGVVAGITPFNFPCMVPCWMFPIAIACGNTFVLKPSERDPSAALRMA
                     QLFAEAGLPAGVFNVVQGDRQAVESLLAHPDVKAVSFVGSSPVAHSIYQHGARAGKRV
                     QALGGAKNHMVVMPDADADQAVDALVGAAYGSAGERCMAISVAVLVGGIADPVVAKLA
                     ERARRLRVGDGMQGDSEMGPVINRPALERIRGYIEDGAACGAELIVDGRVDPASGRPF
                     SVPGYGRGFWLGATLFDRVTPQMRIYREEIFGPVLVCVRVPDFASAVALVNAHPLANG
                     VACFTRDGHVAREFARRIEVGMVGINVPIPVPMAWHGFGGWKGSLYGDTHAYGEEGVR
                     FYTRQKSVMQRWPESTPQGPEFTLPTAK"
     gene            complement(2796..>3466)
                     /locus_tag="VI195_03165"
     CDS             complement(2796..>3466)
                     /locus_tag="VI195_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY6823413.1"
                     /translation="NVPTVLIARTAAEGASLLTSDVDERDRPFIDGARGRTAEGLFAV
                     RAGMAQAIARGCAYTPSVDLVWCETGKPDLAEAREFAAGIHARFPGKLLAYNCSPSFN
                     WKKKLDDAAIARFQRELGAMGYRFQFITLAGFHALNYSMFELARAYKAQHMTAYVRLQ
                     QAEFAAEAAGYTATRHQREVGAGYFDAVTQAVSGGNSAITALSGSTEEQQFEGVAARL
                     GQTG"
BASE COUNT          494 a         1206 c         1265 g          501 t
ORIGIN      
        1 agtagccgcc ctcgaatccg acgccggtgt agccgaatcc atagttgatg ccgccgtaga
       61 agccgacctg cggcccccag tagccggcgt gccacaggta caccccgtcc gcccagcccc
      121 agtatccggg tgtccacagc aacccgaccg gcgccagcag ccaggtgccg ggcacccaga
      181 agtagccgtc aggaccccag gcccagtagc ccggtgtcca gacgaaaccg ggcgcgggac
      241 acggcggctg tacgtatacc ggcagcggcg gcggcgcgaa gttgaccgat acgccgacga
      301 acacgcccgc ctcagcggcg ggcgggagcg ctgccagcag cagcgtgcag gcaagagcgc
      361 ggagcagatt acgcatggct tggctctcct tcgccgggga cggtgaccga gcgatcctga
      421 acgcttcctg aatccgaaag tccgataccg cgttcgcttc ccggcgcatt catctcgtgc
      481 aacgcacccc ggatgggcgc gttgaccgcc ggcgcgccgc ggccagggcg cactcttcag
      541 aaccagccgt agttgaagct gatgctgacg cgctctcccg cgccggcttg cggcggcact
      601 tcgtgccgca accagctttc gaacaacacc agctgtccgc tgcgcgccgt gatcaccacc
      661 cacgggcggc gcgcccgcgc ggcacgggtg cggcgcggcg gcgcggccat gtagcgctcg
      721 aggcgcgggt cctcgagctt cagtcccggc gagccggccg gcaccgcgac gtagtagctg
      781 ccgctgatgg tcgacagcgg atgcaggtgc aggccgtgtg cggcgccgcg tcccatgatg
      841 ttcacccagc agtcggtcat ggcgagcgca cgcccgtcga gatcgaactc gagcgcggcg
      901 gcgaagcgca gtacgcgccg gtcgagctcg cgggcgagcg cggcgaaggt gggcgactgg
      961 cggtgcatgc ggctggccga gccgtaggag gtgtagccgc cggggtagtt gcgcgccgac
     1021 cagcgccggc cggcgccgtc ctcggcgcgc agctgccggc actcgcgcag cagccgtgcg
     1081 ttcagcgccc gccaggacgc gccccgcaac cgctgcgtgt acaccagggt cggaaacaga
     1141 gcctgcacgc tcatggatcg cgcgaagtat gcgggccgtg gccctggtgg tctatgatct
     1201 cgacaccatg gccgtatcca ccgttcctgc ccgcgccccg ggtcacgcgg cgcccgagtc
     1261 cggaagtctc ccgagcgtgc cgcactggat cggcgggcgc gcggtcgccg gtgccggtgc
     1321 gctgctggac gtgttcaatc ccgcgaccgg agaggtcgcg cgccgcgtcg cgctggcggg
     1381 cgaggccgag gtgcacgctg cggtgcagag cgcgcaggcg gcttggcccg cctgggcgga
     1441 cacgccgccg gtgcgccggg cgcgcgtgct ggcgcgcttc atcgcgctgc tcgagcgcga
     1501 gcgcgacgcc ctggcggcgc tcatcaccgc cgagcacggc aaggtgttcg ccgatgccca
     1561 gggcgaagtc acccgcggca tcgaagtggt ggagttcgcc tgcggcatcc cgcagctgct
     1621 caagggggac ttcaccgatc aggtatcgac cggtatcgac aactggacgc tgcgccagcc
     1681 gctcggggtg gtcgccggca tcacgccctt caacttcccc tgcatggtgc cgtgctggat
     1741 gttccccatc gccatcgcct gcggcaacac cttcgtgctc aagccgagcg agcgcgaccc
     1801 gtccgcggcg ctgcgcatgg cgcagctgtt cgccgaggcg ggcctgcccg ccggagtgtt
     1861 caacgtggtg cagggcgacc ggcaggccgt cgagagcctg ctcgctcatc cggacgtgaa
     1921 ggcggtgagc ttcgtcggct cgtcgccggt cgcgcactcg atctaccagc acggggcacg
     1981 cgccggcaag cgcgtgcagg cgctcggcgg ggccaagaat cacatggtgg tgatgcccga
     2041 cgccgatgcc gatcaggcgg tcgatgcgct ggtcggcgcg gcttacggct cggcgggcga
     2101 gcgctgcatg gcgatctcgg ttgcggtgct ggtcggcggg atcgccgacc ccgtggtcgc
     2161 gaagctggcc gagcgcgccc ggcggctgcg ggtcggcgac ggcatgcagg gcgattcgga
     2221 gatgggtccg gtcatcaacc gcccggcgct cgagcgcatc cgcggctaca tcgaggacgg
     2281 tgccgcctgc ggcgccgagc tcatcgtcga cggccgcgtc gatcccgcga gcggccggcc
     2341 gttcagtgtg ccgggctacg ggcgcggctt ctggctcggc gccacgctct tcgatcgcgt
     2401 gacgccgcag atgcgcatct accgcgagga gatcttcggg ccggtgctgg tttgcgtgcg
     2461 cgtgccggat ttcgcctcgg cggtcgcgct ggtgaacgcg cacccgctcg ccaacggggt
     2521 cgcctgcttc acccgcgacg gccacgtggc gcgcgagttc gcacgccgca tcgaagtcgg
     2581 catggtcggc atcaacgtgc cgatcccggt gccgatggcc tggcacggct tcggcggctg
     2641 gaaagggagc ctgtacggcg acacccacgc ctacggcgag gaaggggtgc gcttctacac
     2701 ccgccagaag tccgtcatgc agcgctggcc ggagagcacc ccgcaggggc cggaattcac
     2761 cctgccgacc gcgaagtagc cgcggccggg cggcgtcagc cggtctggcc gagcctggcc
     2821 gcgacgccct cgaactgctg ttcctcggtg ctgccgctga gggcggtgat cgcggagttg
     2881 ccaccgctca ccgcctgggt gaccgcgtcg aaatagccgg cgccgacctc gcgctgatgg
     2941 cgggtggcgg tgtagccggc tgcttcggcg gcgaattccg cctgctgcag ccgcacgtac
     3001 gcggtcatgt gctgcgcctt gtaggcgcgc gccagctcga acatcgagta gttgagcgcg
     3061 tggaagccgg cgagcgtgat gaactggaag cggtagccca tggcgccgag ctcgcgctgg
     3121 aagcgcgcga tcgcggcatc gtcgagcttc ttcttccagt tgaaggacgg tgagcagttg
     3181 taggcgagca gcttgcccgg gaagcgcgcg tggatgcccg cggcgaactc gcgcgcctcg
     3241 gcgaggtcgg gcttgccggt ctcgcaccac accagatcga cagagggtgt gtaagcgcag
     3301 ccgcgcgcaa tcgcctgggc catgccggcg cgcaccgcaa acagcccttc cgcggtgcgc
     3361 ccgcgcgcgc cgtcgatgaa cggccggtcg cgctcgtcga cgtcggaggt gaggagtgag
     3421 gccccctcgg cggcggtgcg tgcaatgagc accgtcggca cgttgc
//
LOCUS       DATDMP010000188         2018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1473019, whole genome shotgun sequence.
ACCESSION   DATDMP010000188 DATDMP010000000
VERSION     DATDMP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473019"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1325
                     /gene="ffh"
                     /locus_tag="VI195_03170"
     CDS             9..1325
                     /gene="ffh"
                     /locus_tag="VI195_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY6823414.1"
                     /translation="MRGQARITEDNVQDMLREVRMALLEADVALPVVRGFIESVKSKA
                     LGSEVAASLTPGQAFVGILHDELIALMGGAGAGFAPRAQPPVVVLLAGLQGAGKTTTA
                     AKLARWLSERERRRVLLASTDVRRPAAMLQLERLAAEVHAGYFPAAAGAAPVAIARAA
                     LERARSGVFDVLIVDTAGRLHVDAELMQEVREIDAAVGAAERLFIVDAMAGQDAVKAA
                     RAFGAALDLTGIFLTKADGDARGGAALSVRQVTGKPIVFIGVGEKTAALELFDPRRMA
                     SRILGMGDVVSLVEQVHRQVDTEEAARLARKVAGGKSFDLADLRGQLEQLQRLGGVGA
                     LLEKLPGAPARQGAVSATQGDRDLRRQIAIINSMTPRERRNPALIDGSRRRRIAAGSG
                     VEVQDVNRLLRQFQQMQRMMKTMKGGGLKRLMGALKGGLPPGFPGR"
     gene            1431..1685
                     /gene="rpsP"
                     /locus_tag="VI195_03175"
     CDS             1431..1685
                     /gene="rpsP"
                     /locus_tag="VI195_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY6823415.1"
                     /translation="MVTIRLTRRGGRNQPFYHVVVTDSRKRPGGPSLEHVGYFNPIAR
                     PGETKLRLDLPRIDYWVGQGAQPSDRVRSLVASFRRQATA"
     gene            1731..>2018
                     /locus_tag="VI195_03180"
     CDS             1731..>2018
                     /locus_tag="VI195_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823416.1"
                     /translation="MAWIELGRLGAPYGVKGWVHVDSYTDPPQGLLQYREWALRLGSG
                     ARITRRVADGRGHGAGLVAQLEGVTDRSGAAALTGAIIEVERAALPRAGPHE"
BASE COUNT          283 a          683 c          777 g          275 t
ORIGIN      
        1 tcaagacgat gcgcggccag gcccgcatca ccgaagacaa cgtgcaggac atgttgcgcg
       61 aggtgcgcat ggcgctgctc gaggccgacg tcgcgctgcc ggtggtgagg ggcttcatcg
      121 agtcggtgaa gtccaaggcg ctcggcagcg aggtggccgc cagcctcact ccggggcagg
      181 cgttcgtcgg catcctgcac gatgagctca tcgcgctcat gggcggggcg ggggcgggct
      241 tcgcgccgcg ggcgcagccg ccggtggtgg tgctgctcgc ggggctgcag ggcgccggca
      301 agacgaccac cgcggccaag ctcgcccgct ggctgagcga gcgcgagcgc aggcgcgtgc
      361 tgctcgcgag caccgacgtg cgccggccgg cggcgatgct gcaactcgag cggctcgcgg
      421 ccgaggtgca tgccgggtac tttcccgccg ctgccggcgc agctccggtg gcgattgccc
      481 gtgcggcgct cgagcgggcg cgcagcggcg tcttcgacgt gctgatcgtc gacaccgccg
      541 gccggctgca cgtcgacgcg gagctgatgc aggaggtgcg cgagatcgac gccgcggtgg
      601 gcgccgccga gcggctgttc atcgtcgatg ccatggcggg ccaggatgca gtgaaggccg
      661 cgcgcgcctt cggggcggcg ctcgatctga ccggcatctt tctcaccaag gccgacggcg
      721 atgcccgcgg cggtgcggca ctctcggtgc ggcaggtgac cggcaagccg atcgtgttca
      781 tcggcgtcgg cgagaagacc gcagcactcg agctgttcga tccgcggcgc atggcatcgc
      841 gcatcctcgg catgggcgac gtggtgagcc tggtcgagca ggtccaccgc caggtcgata
      901 ccgaggaggc ggcccggctc gcgcgcaagg tcgccggggg caagagcttc gatctggcgg
      961 acctcagagg ccagctcgaa cagctgcagc ggctgggggg cgtcggggcg ctgctcgaga
     1021 agctgcccgg cgcgccggcg cggcagggcg cggtgtccgc cacccagggt gaccgggacc
     1081 tgcgccgcca gatcgccatc atcaattcca tgacgccccg cgagcgacgc aacccggccc
     1141 tcatcgacgg ctcgcgccgc cggcgcattg cggcgggctc cggggttgag gtgcaggacg
     1201 tgaaccgcct cctcaggcag ttccagcaga tgcagcggat gatgaagacc atgaaaggcg
     1261 ggggcctcaa gcgcctcatg ggcgccctca agggagggct gcccccgggg ttccccggcc
     1321 gctgaggtgc gaaactggcg caggattcgc cggttgcgta gaatccgcgc tcttttttgg
     1381 gcggccgccc cgggggcggc cacccgggcc ggaacgagag cgatacgacc atggtgacca
     1441 tacgactgac gcgccgcgga gggcgcaacc agcccttcta tcacgtcgtc gtgaccgaca
     1501 gccgcaagcg tccgggtggc ccgagcctcg agcacgtcgg ctatttcaac ccgatcgcgc
     1561 ggccgggtga gacgaagctc aggctcgatc tgccgcggat cgactactgg gtcgggcagg
     1621 gcgcgcagcc ctccgatcgc gtgcgctcgc tggtggcctc cttccgcagg caggcgaccg
     1681 cctgagtcgc gaggtccgcc gggcgcagcg cactcgagcg atgagccgcc gtggcctgga
     1741 tcgagctcgg ccgtctcggc gccccgtacg gcgttaaggg ctgggtacac gtcgactcgt
     1801 atacggatcc cccgcagggt ctgctgcaat accgcgagtg ggccctgcgg ctcgggagcg
     1861 gggcgcggat cacgcgccgg gtcgccgacg ggcgcggaca cggtgccggc ctggtggcgc
     1921 agctcgaggg cgtgaccgac cgcagcggcg ctgcggcgct cacgggtgcg atcatcgagg
     1981 tggagcgcgc ggcgctgccg cgcgccgggc cgcacgag
//
LOCUS       DATDMP010000189         2283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_918330, whole genome shotgun sequence.
ACCESSION   DATDMP010000189 DATDMP010000000
VERSION     DATDMP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918330"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VI195_03185"
     CDS             <1..1522
                     /locus_tag="VI195_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY6823417.1"
                     /translation="ALAGSPPFARAALLLARYYNDSGRAAEALAVAAPMCLSGRGDAE
                     LAAQHIAALAALGRQQEALATYRALVVARPENPALAHALATALNTSGQYEEASRLAQQ
                     VLNRGHRSAALLNTYARSLIAQGELERAEAALRECLALEPRLADAHSHLAQLIWLRSG
                     DLAQATAELEQALQTFTTDEALWAAKAAILQGGGDARAAYACLAPWAARAQAPPALLL
                     RAGLAALEFDAGQALELAERALRVVPSNAAARTLLVAAQLGTGNARAALTECEALLAR
                     SPDDQYLIALQTTAWRLLGDARYAQLCDYRSLVVPRPLEPPPPWRDLTEFLADLRVSL
                     NRLHDPHGHALLFQSLRNGTETTQDLSRSSDPVIQALFAAFAPLIRHYLEQIGRGNDP
                     LRRRNNGRWRFNGSWSVRLHSAGYHTNHTHPRGWISSACYIELPECMSAGRSDEGILT
                     FGAPGIATNPALGAEYSVTPAAGMLVLFPSYFWHGTVPFRSAQARLTVAFDAVPER"
     gene            complement(1636..>2283)
                     /locus_tag="VI195_03190"
     CDS             complement(1636..>2283)
                     /locus_tag="VI195_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY6823418.1"
                     /translation="GPAQLTPAPAPVAAPALKSHDLFPTRIWQAHLDTLEPYMERWVQ
                     AVLAMRAAHPEPAGRTVRQGWNSEDMKVLEQAEFAPLRDVIRAACTHALREMGQGERA
                     FYLQSWINLHDRGGFNFLHMHEGSLLSGSFYLQVPPGSGEFVFRDPRPGVIHGYVKGG
                     VPNGHADVHLRPSDGLLVLFPCWMEHYVEPHEGDEPRITVAFNANPLEPEIHGQR"
BASE COUNT          353 a          803 c          768 g          359 t
ORIGIN      
        1 ggcgctcgcc gggtcgcctc cttttgcgcg cgcggctctc ctgctcgcgc gctactacaa
       61 cgacagcggg cgagctgccg aggcgctcgc ggtggcggcc ccgatgtgcc tgtccgggcg
      121 cggcgacgcg gagctcgccg cgcagcacat cgcggcgctc gctgccctgg gacgtcagca
      181 ggaggccctt gcgacctacc gggcgctcgt ggtcgcgcga ccggagaatc cggctctggc
      241 gcacgcgctc gcgaccgcgc tgaacacgag cggtcagtac gaggaagcca gccgactcgc
      301 gcagcaggtg ctcaaccgcg ggcaccggag cgcggcgctc ctcaacacct atgcgcgcag
      361 cctgatcgcc cagggtgagc tcgagcgcgc cgaggcggcg ctgcgcgagt gcctggcgct
      421 cgagccgcgt ctcgccgatg cgcacagtca tttggcacaa ctgatctggc tgcgcagcgg
      481 agatctcgcg caggcgaccg ccgagctcga gcaggcattg cagaccttca cgaccgatga
      541 agcgctctgg gcggccaagg cggcgatcct ccagggcggc ggcgatgcgc gcgccgcgta
      601 cgcgtgcctc gctccctggg ccgcgcgtgc gcaggcgccg cccgcgctgc tgctgcgcgc
      661 cgggcttgcg gcgctggagt tcgacgcggg ccaagcgctg gagctggccg agcgcgcgtt
      721 gcgggtcgta ccctccaacg ccgccgcacg cacactgctc gtggccgcgc agctcggtac
      781 cggcaacgca cgcgcagcac tcacagagtg cgaggcgctg ctcgcacgca gccccgatga
      841 tcagtacctc attgcgctgc agacgaccgc ctggcggttg ctcggcgatg cgcgctacgc
      901 gcagctctgc gactaccgca gtctcgtcgt gccgcgaccg ctcgagccgc cgccgccgtg
      961 gcgggacctg acggaatttc ttgccgacct cagggtgagc ctcaaccggc tgcacgaccc
     1021 gcacgggcat gcactgctgt ttcaatcgct gcgcaatggc accgagacga cgcaggatct
     1081 ctcccgcagc tccgatccgg tgatccaggc actgttcgcg gcctttgcac cactcatccg
     1141 ccactacctc gagcagatcg gtcgggggaa tgacccacta cgccggcgca ataacggccg
     1201 ctggcgtttc aacggcagct ggtcggtgcg gctgcacagc gcgggctatc acaccaacca
     1261 cacgcatcct cgcggctgga tctcatcggc ctgctacatc gagcttcccg agtgcatgag
     1321 cgctgggcgc agcgatgagg gcatactgac cttcggtgcg cctggcatcg ccaccaaccc
     1381 ggcgctcggc gccgaatact cggtaacacc cgcagccggc atgctggtgc tctttccctc
     1441 ctatttctgg cacgggaccg tgcccttccg gagcgcccag gcgcggctca cggtcgcctt
     1501 cgatgcggtg cccgagcgct agctggccgg gtggcgcagc gagtgcagtg agggtgtttt
     1561 cggcgatagt taaccgttcg gtaaaccatt cataagagat gtatggacta gtcccggggg
     1621 gtccgcgcac ggggttcaac gctggccgtg gatctcgggt tccagcgggt tcgcattgaa
     1681 tgcgacggtg atgcgcggct catcaccctc gtgcggctcg acgtaatgct ccatccagca
     1741 gggaaagagc accagcagtc catcgctcgg ccgcaggtgc acatcggcat gtccgttggg
     1801 cacgcctccc ttgacgtagc cgtgaatcac cccgggccgc ggatcgcgga acacgaattc
     1861 ccccgacccg ggcggcacct gcagatagaa gctcccggag agaagcgagc cctcgtgcat
     1921 gtgcaggaag ttgaatccgc cgcgatcgtg caggttgatc caggattgca ggtagaaggc
     1981 gcgctcgccc tggcccatct cgcgcagcgc gtgggtgcac gccgcgcgaa tcacatcgcg
     2041 caacggcgcg aattccgcct gctcgagcac cttcatgtcc tcgctgttcc agccctggcg
     2101 gacggtccga cccgcgggct cggggtgcgc cgcacgcatc gccagcacgg cctgcaccca
     2161 acgctccatg tagggctcga gcgtatcgag atgcgcctgc cagatgcggg tcggaaagag
     2221 atcgtgcgat ttgagcgccg gtgccgccac aggcgcgggt gcgggcgtga gctgcgccgg
     2281 acc
//
LOCUS       DATDMP010000190         3363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2140523, whole genome shotgun sequence.
ACCESSION   DATDMP010000190 DATDMP010000000
VERSION     DATDMP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140523"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VI195_03195"
     CDS             <1..732
                     /locus_tag="VI195_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY6823419.1"
                     /translation="MVARGDLGVEMGYAELTGLQKTIIHETRTRNKVVITATQMMESM
                     ISNPVPTRAEVSDVANAVMDGTDAVMLSAETATGRHPVKAVEAMAQVIEGAEKYQLTH
                     SRARHRTSEGQFRVTEEAIAMAVMYTANHMKVRAIVALTESGATPLWMSRIRADIPVY
                     AFTRNEATRRRVTLYRGVYPVIFDVTAGGGSTEGLYSALFARLLELQLVQERDLVILT
                     KGEHSGVQGGTNSMQILTVALGPPR"
     gene            754..3114
                     /locus_tag="VI195_03200"
     CDS             754..3114
                     /locus_tag="VI195_03200"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY6823420.1"
                     /translation="MRTLKFAALAGALLVLVVCGTLYGLLRASLPQLDAKRHAAGLAA
                     AVRITRDALGVPTIEAGNRLDLAYATGFAHGQDRFFQMDIARRFAAGELAELFGPVAL
                     DQDRQTRLFRFRAVAREVLAAATPAQRALLDAYARGVDAGLASLRVRPWEYLLLRATP
                     LPWRSEDSVLVEHAMWWDLQVNGLRREMLRQEINARLGGPQCADGWKCALGFLYPAGT
                     SWDAPGDATAVAAAAQPVPDAAALDVRGTPSFPAPDPGVRTPAAGSNSWAVAGRLTAS
                     GAALIANDMHLGQRVPPTWYRARLRVAAHGSEPQLDLNGVTLPGAPLMVAGSNGHIAW
                     GFTNSYGNWFDVAAAPCAPGDAPLTVVHEEIRVRGAPSVGLDISSGPLGVLWRNDARG
                     DRCWFVAWLAQLPEATNLGLMELEHVGSVAEALAIAPAIGIPHQNVVLGDREGHIGWT
                     IFGRIPAQTGSGRARAHGPWTTAADHPRSVDPPLGRLWTANARVASDPRQLQLIGGDL
                     ASLGAEYDLGARASQIRDDLMAVAAPVTPADMLRIQLDDRALFLGRWQALLLRLLDAR
                     ALQNQPGRAQFRTLIEHWNAQASVDAVGYRLVRAYHDRAQQAVWDMLLAALRIPGDDA
                     SWPPGQFEGPLWELVTTQPLHLLAPDYRDWREFLLAQVDATLAELAPPCPQLARCTWG
                     AHNLARIRHPLSRALPWLASFLDMPSLELAGDHDMPRVQDAAFGQSERFAVAPGHEGE
                     GYLHIPGGQSGHPLSPYYRAGFDAWARGQPLPFLPGPAQHTLTLMP"
     gene            complement(3116..>3363)
                     /locus_tag="VI195_03205"
     CDS             complement(3116..>3363)
                     /locus_tag="VI195_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823421.1"
                     /translation="PTLRGVLEFLELEWNEHVRRYSETARKRSISTPSAAQVVRPLYR
                     SAQGKWRNYREFLEPYLPALAPWVTAFGYASEPAEGR"
BASE COUNT          500 a         1175 c         1164 g          524 t
ORIGIN      
        1 atggtggcgc gcggcgatct cggcgtggag atgggttacg ccgagctcac cgggctgcag
       61 aaaaccatca tccacgagac ccgcacgcgc aacaaagtcg tgatcaccgc gacgcagatg
      121 atggagtcga tgatcagcaa cccggtgccg acgcgcgccg aggtgagcga cgtcgccaac
      181 gcggtgatgg acggtaccga tgcggtcatg ctgtcggccg agaccgccac cggacgccac
      241 ccggtgaagg ccgtggaggc gatggcacag gtcatcgagg gcgccgagaa gtaccagctt
      301 acccacagcc gggcgcgtca ccgcaccagc gaggggcagt tcagggtcac cgaggaggcg
      361 atcgccatgg ccgtcatgta cacggccaac cacatgaagg tgcgtgccat cgtggcgctg
      421 acggaatcag gcgcgactcc gctgtggatg tcgcgcatcc gcgccgatat ccccgtatat
      481 gccttcacgc gtaacgaggc gacgcgccgg cgcgtgacgc tctaccgtgg cgtttacccg
      541 gtgatattcg atgtgaccgc ggggggcggc tccaccgagg gcttgtacag tgcgctgttc
      601 gcgcggctgc tggagctgca actggtgcag gagcgtgacc tggtgattct caccaagggc
      661 gagcactcgg gcgttcaggg tggcaccaac tccatgcaga tcctcacggt cgccctgggt
      721 cccccgcgct aggcgacgct ccctccgcgc gcgatgcgca cgctcaaatt cgcggcattg
      781 gccggcgcac tgctcgtgct ggtagtctgc ggcacccttt acgggctgct gcgcgcttca
      841 ctcccgcaac tcgacgcaaa gcggcacgcc gccggcctcg ccgcggcggt gcgcattacg
      901 cgcgatgcgc tcggggtgcc gaccatcgag gcgggcaacc ggctcgacct ggcgtacgcc
      961 accggctttg cccacggcca ggatcggttt ttccagatgg acatcgcgcg gcgcttcgcc
     1021 gccggggagc tggcggagct cttcggcccg gtcgcgctcg atcaggaccg gcaaacgcgc
     1081 ctgttccgct tccgcgcggt cgcgcgcgag gtgctggctg ccgccactcc cgcacagcgc
     1141 gcgctgctcg atgcatatgc gcgcggcgtc gacgcgggac tcgccagcct gcgcgtccgc
     1201 ccgtgggagt atctgctgct gcgcgcgacg ccgctcccct ggcgcagcga ggacagcgtg
     1261 ctggtcgaac acgccatgtg gtgggatctg caggtgaatg gcctgcgccg cgagatgctg
     1321 cggcaggaaa tcaacgcgcg gctcggcggt ccgcagtgtg cggacggttg gaaatgcgca
     1381 ctcggcttcc tgtatccggc cggcacgagc tgggatgctc cgggcgacgc cacggcagtt
     1441 gccgccgccg cacagcccgt gcccgatgcg gcggcgctcg atgtgcgcgg cacgccgtct
     1501 tttcccgcac ctgatcccgg cgtgcgcact ccggccgccg gcagcaacag ctgggcggtc
     1561 gcgggacggc tgaccgcgag cggcgcagcg ctgatcgcca acgacatgca cctcggacag
     1621 cgcgtgccac ccacctggta tcgcgcccgt ctgcgcgtcg cggcgcacgg cagcgagccg
     1681 cagctcgatt tgaacggcgt cacgctgccc ggtgccccgc tgatggtagc cggatcgaac
     1741 ggccacatcg cctggggctt caccaacagc tacggcaact ggttcgacgt cgcggccgcg
     1801 ccgtgcgcgc cgggcgatgc gccgctcacg gtggtgcacg aagagatccg cgtgcgcggc
     1861 gcgccgagcg tgggactcga catcagcagc gggccccttg gagtgttgtg gcgcaacgat
     1921 gcgcgtggcg atcgttgctg gttcgtcgcg tggctggcgc agctccccga ggcaaccaac
     1981 ctcggcctga tggagctcga gcacgtcggc tcggtcgcag aggcgctggc gatcgcgccg
     2041 gccatcggca ttccacacca gaacgtggtg ttaggcgacc gcgaaggtca catcggctgg
     2101 acgatcttcg gccgcatccc ggcacagacg ggcagcggac gcgcgcgtgc gcacggaccg
     2161 tggactaccg cggccgacca tccgcgcagc gtggaccccc cgctcggccg cctgtggacg
     2221 gctaacgccc gggtggccag cgatccgcgc cagctgcagc tgatcggtgg ggatctggcg
     2281 agcctcggcg ccgagtacga cctcggcgca cgcgccagcc agatccgtga cgacctcatg
     2341 gcggtcgccg cgccggtcac gcccgccgac atgttacgca tccagctcga tgaccgcgcc
     2401 ctgttcctcg gccgctggca ggcgctgcta ctacggctgc tcgatgcgcg cgcactgcag
     2461 aaccagccgg gcagagcgca gttccgcacg ttgatcgagc actggaacgc acaggccagt
     2521 gtcgatgcgg tgggctatcg cctggtgcgc gcttatcacg accgcgcgca gcaggcggta
     2581 tgggacatgc tgctcgcagc gctgcgcatc cccggcgacg atgccagctg gccgcctggg
     2641 cagttcgagg ggccgctgtg ggagttggtg acgacccagc cgctgcacct gctggcgccg
     2701 gattatcgcg actggcgtga gttcctgctg gcgcaggtgg atgcgacgct tgccgagctc
     2761 gcgccgccct gcccgcagct cgcccgctgt acctggggcg cgcacaatct cgcgcgcatc
     2821 cggcacccgc tgtcgcgcgc gctgccgtgg ctcgcgtcgt tcctcgacat gccgtcgctc
     2881 gagttagcgg gcgatcacga tatgccccgt gtccaggatg ccgccttcgg ccagtcggag
     2941 cgttttgcgg tcgcaccggg acacgagggc gagggctacc tgcacattcc gggcggacag
     3001 agcgggcacc cgctctcgcc ctactaccgc gccggtttcg atgcctgggc acgggggcaa
     3061 cccctgccgt tcctgccggg ccctgcgcaa cacacactca cgctgatgcc gtgactcaac
     3121 ggccctcggc tggctcgctg gcatatccaa aggcggtgac ccacggcgcc agagccggca
     3181 gatagggttc gaggaattcc cggtagttgc gccacttccc ctgcgcactg cggtagagcg
     3241 ggcgcaccac ctgcgccgcg ctcggggtgc tgatgctgcg cttgcgtgcg gtttcgctgt
     3301 accggcgcac atgctcattc cattcgagct cgagaaactc cagcacgccg cgcagagtcg
     3361 gct
//
LOCUS       DATDMP010000191         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1362916, whole genome shotgun sequence.
ACCESSION   DATDMP010000191 DATDMP010000000
VERSION     DATDMP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362916"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..569
                     /locus_tag="VI195_03210"
     CDS             354..569
                     /locus_tag="VI195_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823422.1"
                     /translation="MRTTTWAILGSLAINLMLALAIAAVFSASPARAAETAQCSTHAA
                     ATGRQAPAQPARSGHRYIVAVRMGWMG"
     gene            complement(580..1509)
                     /locus_tag="VI195_03215"
     CDS             complement(580..1509)
                     /locus_tag="VI195_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823423.1"
                     /translation="MSATKAGVLVALVLVNIAFLIGWLWAVRRYRLRGRPDLPDIGIG
                     CLTSFLDTLGIGSFAPTTALFKFRGRPADEFIPGTLNVGLNPAAFIETLLFISAVAVQ
                     PLLLVCMVATATAGAWLGAGVVSRMRRRSIQLFMGVALLIAAFFFAMTNIHELSIRHG
                     NFAHFLDIFPAGGEAFGLSGWRFIVAVSVSFVLGALMCIGIGNYAPLMILLALLGMHP
                     LAAYPIMMASDGMLQPVASLGFFRSGRFSHVSSLGLTVGSFVGVPVAFYLVKNLLPIS
                     IMRWLVTFVVAYAAVSMLRSARTPAAPASVAVG"
     gene            complement(1518..>2182)
                     /locus_tag="VI195_03220"
     CDS             complement(1518..>2182)
                     /locus_tag="VI195_03220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6823424.1"
                     /translation="IDTPQGSVSAPVVIIATDAYTDAISDELRRTMVPVPSFQVATAP
                     IPAELRSTILPEGQAASDTWHLLRYFRLDTSGRLVLGSRGTFAQSPQLRDVQYHYHAV
                     HELYPQLKGIPFEYHWGGLVAMTQDQLPHLHEPAPGLLAGLGYNGRGVAMATVLGRVL
                     ARRALGAQVAELGFPVSPVVPLPFHALSGVGARATIQYLRCIDGLARLSSRLRTHVPF
                     RA"
BASE COUNT          431 a          721 c          722 g          308 t
ORIGIN      
        1 tgcgagacgg catgcttacc ctgcttggcg cactccgcct cggcctgctc gagcgcccgc
       61 gcctcgcgcc gttcatcgac atcggggcgt ccggctccgg gcgcggaagg gttgtcaggc
      121 gatggaccgg ccgcgcaggc agcgagcagg gcgatgcagg gcaagaccat ccttttcacg
      181 ttgcctccat ccaattccgt tggctcccgg agcgcatcgc ccgcttgtcg cattagtgta
      241 acactgcggc gggacgaacc cgttggcggc gaggatggat ccgctggcac accactgacg
      301 ccggagcggc cccgggcgtc tgatatgcac ggcgaaaata cggggagaga ggcatgagga
      361 ccacgacctg ggcgatcctc ggatcgctgg caatcaacct gatgctggcg ctggcgatcg
      421 ccgccgtgtt cagcgcatcg ccggcgcggg cggcggaaac ggcgcagtgc tcgacgcatg
      481 cggccgcgac cggccgacag gcaccggccc agccggcccg ttcaggacac cgctacatcg
      541 ttgccgtgcg catgggctgg atgggctgat cgagccgcat cagcccaccg cgacactcgc
      601 cggcgccgcc ggcgtgcgcg ctgagcgcag catcgaaacc gccgcgtagg cgaccacgaa
      661 cgtcaccagc cagcgcatga tcgatatcgg cagcaggttc ttcacgaggt agaaagccac
      721 cggcactcca acgaaactgc cgaccgtgag gccaagtgag gacacgtggg aaaagcggcc
      781 cgagcggaag aacccgaggc tcgccactgg ctgcagcatg ccgtcgctcg ccatcatgat
      841 ggggtacgcg gcgagcggat gcatgccgag cagcgcgaga aggatcataa gcggtgcgta
      901 gtttccaatt ccgatgcaca tcagggcgcc cagcacgaag ctcacgctca ccgcgacgat
      961 gaaacgccag ccggagagcc cgaatgcctc gccccccgcc ggaaaaatgt cgaggaagtg
     1021 ggcgaagttg ccgtgccgaa tggatagctc gtgaatattc gtcatcgcaa agaaaaaggc
     1081 agcgatcagc agcgccacgc ccatgaacag ctgaatcgag cgtcgccgca tacggctcac
     1141 gacccctgct ccgagccacg caccggcggt cgcagtcgcc accatgcaca cgagcagcag
     1201 cggctgtacg gcgaccgccg aaatgaacag caatgtctca atgaaggctg ccgggttgag
     1261 cccgacgttg agcgtaccgg ggatgaactc gtcagccggc ctgccacgaa acttgaacag
     1321 cgccgtggtc ggggcgaacg acccgatgcc aagcgtatcg aggaagctcg tgaggcagcc
     1381 gataccgatg tccgggaggt ccggacgacc gcgcagccgg taccggcgca cggcccacag
     1441 ccagccgatg aggaaggcga tgttgaccag cacgagtgcc accagcacac cggctttcgt
     1501 cgcactcatc gataccccta tgccctgaaa ggcacgtgcg tccgcagccg gctgctgagc
     1561 cgtgcgagcc catcgatgca acgcaagtac tggatcgtgg cgcgcgcacc gacgccgctc
     1621 agtgcatgga acggcagcgg cacgaccggc gagacgggga agccaagctc cgccacctgc
     1681 gcgccgagcg cacgccgcgc cagcactcgc cccagcaccg tcgccatcgc cacgccgcgg
     1741 ccgttgtaac cgagtcccgc cagcagtccg ggtgcgggtt cgtgcagatg gggcagctgg
     1801 tcctgcgtca tggccaccag gccgccccag tggtactcga aaggaatgcc tttgagctgc
     1861 ggatagagct cgtgcaccgc atgatagtga tactgcacat cgcgcagctg cggtgactgg
     1921 gcgaaggtgc cccgcgagcc gagcacgagg cgtccgctcg tatcgagtcg gaagtaacgc
     1981 agcagatgcc aggtgtcgga agcagcctgc ccctcgggca gtatcgtgct gcgcagctcc
     2041 gctggaatag gtgcagtcgc cacctggaag gacggcacgg gtaccatcgt ccgccgcagt
     2101 tcatcgctga tggcgtcggt gtaggcatcg gtcgcgatga tgactacggg agcgctcacg
     2161 ctcccctgag gcgtatcgat gg
//
LOCUS       DATDMP010000192         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_30167, whole genome shotgun sequence.
ACCESSION   DATDMP010000192 DATDMP010000000
VERSION     DATDMP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30167"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VI195_03225"
     CDS             complement(<1..500)
                     /locus_tag="VI195_03225"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HEY6823425.1"
                     /translation="MAKKKTIRTIPQERTPMREQAPAARVKNFTEVALGYSLEEALRE
                     SERCLLCPEQPCVDGCPVGIDIPGFIQRIGQKDFRGAFDILTDTNLLPAICGRVCPQE
                     NQCEGPCPVGTQFEPVAIGRLERFVGDMAIAEKWTSVPYIEKNGLKVGIVGSGPAGMA
                     CAADMA"
     gene            complement(505..1419)
                     /locus_tag="VI195_03230"
     CDS             complement(505..1419)
                     /locus_tag="VI195_03230"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEY6823426.1"
                     /translation="MAREDFSDVTYLLEVRHPLMAKAARPGQFVIVMSHAEGERIPLT
                     LADYDRGKGTITLVIQAVGKTTREMQQTCKVGTRLYGVAGPMGIPSHISKVKKVACVG
                     GGLGVAPVFPHARAFKENGAHVIGIIGFRNKDLMFWQDKFGAVCDEFIVCTDDGSAGI
                     KGNVTAGIRKALAAHADIDEFVAIGPPIMMKGCAEATRERKVKTMVSLNPLMVDGTGM
                     CGGCRVKIGGKVKFACVDGPDFDGHQVDFDDLMTRLKRFSAQEKTAVERWSEHCRMTD
                     PLHTLKTSMSKSAEALELPDPFDEVRGG"
     gene            complement(1529..>2458)
                     /locus_tag="VI195_03235"
     CDS             complement(1529..>2458)
                     /locus_tag="VI195_03235"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY6823427.1"
                     /translation="ASGSWTNPMSSPASAAEQSSPGAAARVAAELVAGGSLEIGAHRP
                     QDARAIAALLPSGTPVYVNHLPRHRLLDTLPTLAAVRAAGLEPVPHIAARRIRDRAEL
                     QAFLARATGEAGVRKALILGGDEPEAIGPYADGAALLREDLLAASGLREIGLPGYPEG
                     HPRIPAGALEQAFAEKLLLAARQGMGTYVVTQFSFAPSRVVEFCARMARSAPALPVYV
                     GLAGPTNPVALLRFAQRCGVSASLRALRTQGMDAVRLVTHTDPADQLAAIARYCAAHA
                     ACNVVGIHLFTFGGVAASAEWMNRYIASHGGLP"
BASE COUNT          356 a          842 c          876 g          384 t
ORIGIN      
        1 ttggccatgt ccgcggcgca ggccatgccg gcggggccgg agcccacgat gccgaccttc
       61 aggccgttct tctcgatgta gggaacgctg gtccacttct cggcaatcgc catgtcgccc
      121 acgaagcgct ccagccgccc gatggcgacg ggctcgaact gcgtgcccac ggggcacggc
      181 ccctcgcact ggttctcctg cgggcagacg cggccgcaga tggccggcag caggttggtg
      241 tcggtgagga tgtcgaaggc gccgcggaaa tccttctggc cgatgcgctg gatgaagccc
      301 gggatgtcga tgccgaccgg gcagccgtcc acgcagggct gctccgggca cagcaggcag
      361 cgctcggact cgcgcagcgc ctcctcgagg ctgtagccca gcgccacctc ggtgaagttc
      421 ttgacgcgcg ccgcgggcgc ctgctcgcgc atcggcgtcc gctcctgcgg aatggtgcgg
      481 atggttttct tcttcgccat gggattagcc gcctctcact tcgtcgaacg ggtcgggcaa
      541 ctccagcgcc tcggccgatt tcgacatgct ggtcttcagg gtgtgcagcg gatcggtcat
      601 gcggcagtgc tccgaccagc gctcgaccgc ggtcttctcc tgcgccgaaa agcgcttcag
      661 tcgcgtcatg aggtcgtcga aatcgacctg gtggccgtcg aagtccgggc cgtccacgca
      721 ggcgaacttc actttcccgc cgatcttcac gcggcagccg ccgcacatgc cggtgccgtc
      781 gaccatgagc ggattgaggc tcaccatggt cttgaccttg cgctcgcgcg tggcctcggc
      841 gcagcccttc atcatgatcg gcgggccgat ggcgacgaac tcgtcgatgt ccgcgtgcgc
      901 ggcgagcgcc ttgcggatgc ccgcggtcac gttgcccttg atgccggccg agccgtcgtc
      961 ggtgcaaacg atgaattcgt cgcagaccgc gccgaacttg tcctgccaga acatcaggtc
     1021 cttgttgcga aagccgatga tgccgatgac gtgggcgccg ttctccttga acgcgcgcgc
     1081 gtgcgggaac acgggcgcca cgcccaggcc gccgccgacg caggccacct tcttcacctt
     1141 gctgatgtgg ctcgggatgc ccatcggccc ggccacgccg tacaggcggg tgccgacctt
     1201 gcaggtctgc tgcatctcgc gggtggtctt gccgaccgcc tggatgacca gggtgatggt
     1261 gcccttgccg cggtcgtagt ccgcgagcgt gagggggatg cgctctcctt cggcgtgcga
     1321 catgacgatc acgaactggc cggggcgggc cgccttcgcc atcagcggat ggcgcacctc
     1381 gagcaggtag gtgacgtcgg aaaaatcctc tcgcgccacg atttcatagt cggccatagt
     1441 ccgcatcctc tttggggagc agcctatgcc cggtcagggc gccggacttg atctaacgca
     1501 aatagggcgg ggatggatcc gcgagaatct agggcagtcc gccgtgcgaa gcgatgtagc
     1561 ggttcatcca ttcggcggac gcggcgacgc cgccgaacgt gaacaggtgg atgccgacca
     1621 cgttgcaggc cgcgtgcgcg gcgcagtagc gcgcgatcgc ggcgagctgg tcggccgggt
     1681 cggtatgcgt caccaggcgc accgcgtcca tgccctgcgt gcgcagcgcg cgcagcgaag
     1741 cgctcacgcc gcagcgctgc gcgaaacgca gcagcgcgac cgggttggtc gggccggcga
     1801 ggccgacgta gaccggcagc gccggcgcgc tgcgcgccat gcgcgcgcag aattcgacca
     1861 cgcgcgacgg cgcgaacgaa aactgcgtga cgacgtaggt gcccatgccc tgcctcgccg
     1921 cgagcagcag cttctcggcg aacgcctgct ccagcgcgcc ggccggaatg cgcggatggc
     1981 cttccgggta gccgggcagg ccgatctcgc gcaggccgct ggcggccagc agatcctcgc
     2041 gcagcagcgc cgcgccgtcg gcgtagggcc cgatcgcctc gggctcgtcg ccgcccagga
     2101 tcagcgcctt gcgcacgccg gcttcgcccg tcgcgcgcgc gaggaacgcc tgcagctcgg
     2161 cgcggtcgcg gatgcggcgc gcggcgatgt gcggcacggg ctccaggccc gccgcgcgca
     2221 ccgcggccag ggtggggagg gtgtcgagca ggcggtggcg cggcaggtgg ttgacgtaga
     2281 ccggagtccc cgagggcagc agcgcggcga tggcgcgcgc atcctgcggg cggtgcgcgc
     2341 cgatctccag cgagccgccg gccacgagct cggcggcgac ccgggcggcc gcgcccggcg
     2401 aactttgctc agccgcgctt gcgggagacg acataggatt cgtccaggaa ccagaggc
//
LOCUS       DATDMP010000193         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1140431, whole genome shotgun sequence.
ACCESSION   DATDMP010000193 DATDMP010000000
VERSION     DATDMP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140431"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VI195_03240"
     CDS             complement(<1..187)
                     /locus_tag="VI195_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEY6823428.1"
                     /translation="MYAEFGDKLDLETNQSLKALAIALRARALPWIRDVVPALCGIAV
                     HFDPFHPELPASVLEYFQ"
     gene            complement(235..828)
                     /gene="nthA"
                     /locus_tag="VI195_03245"
     CDS             complement(235..828)
                     /gene="nthA"
                     /locus_tag="VI195_03245"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEY6823429.1"
                     /translation="MKDSGSYQPFSYFQLMEVSLRELLVEKGIVTEAQVAATVEDMRQ
                     RSPERGARVVARAWVSDAFRKKLLENGTQACEELGLDVPALKLVVVENTPDVHNMIVC
                     TLCSCYPRMLLGIPPDWYKSRNYRSRAVNEPRAVLSEFGLSLSEETRIRVHDSTADMR
                     YLVLPMRPAGTEGWSEERLAAIVTRDCMIGVAVPRAN"
     gene            complement(839..1120)
                     /locus_tag="VI195_03250"
     CDS             complement(839..1120)
                     /locus_tag="VI195_03250"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEY6823430.1"
                     /translation="MVETRFKPGDRVTVRRMYPLGHIRTPFYIRGHSGVIERCCGTFG
                     NPEELAQQRSGEPKQPLYRVRFLQKDVWPQYRGNPQDVLEVEIFQHWLE"
     gene            complement(1092..1430)
                     /locus_tag="VI195_03255"
     CDS             complement(1092..1430)
                     /locus_tag="VI195_03255"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823431.1"
                     /translation="MIERSHHDMGGLPAGRVEPTEHDYAEWERRVDALMMLLSRVKDG
                     RKLLTVDELRKNIEALPPDAYDRMSYYERWVTSITQTMIQRGVITTEELGRKMAEIQA
                     KNGRNKIQAR"
     gene            1449..>2211
                     /locus_tag="VI195_03260"
     CDS             1449..>2211
                     /locus_tag="VI195_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6823432.1"
                     /translation="MAVAAQQVPPPGNLFLDHVSHFVPDLGAAARALEALGFAVTPVS
                     AQQTQAGPAGTSNVCVMLERGYLEFLAPTMDTPNAQRLRATMQRYAGVHLCCFGTPDA
                     EGEHRRLRAHGFSPPPVVNLQRETAEEKVAKFHVVRAAPERMPEGRIQFVQHLAPEAI
                     WRPQCLGHTNNVVKLACLFVVADETVDAAARWAHFAALLPRPAGGYVHLQAARGHVLV
                     GTRAQWRELLGGAPAAPALAGYALECRDPAILIARC"
BASE COUNT          338 a          763 c          724 g          386 t
ORIGIN      
        1 cctggaaata ctccagcacc gaggccggca gctccgggtg gaaggggtcg aaatgcacgg
       61 cgatgccgca cagcgccggc accacgtcgc ggatccaggg cagcgcgcgc gcgcgcagcg
      121 cgatcgccag cgctttcagc gactggttgg tctcgagatc gagcttgtcg ccgaattcgg
      181 catacaccgc cgcgtcgccg agcggcgaga ggcgcagggt cttgaggagc tttttcagtt
      241 tgccctgggt accgcgacgc cgatcatgca gtcgcgcgtc acgatcgccg ccagccgctc
      301 ttcgctccag ccttcggtgc cggccgggcg catcggcagc accaggtagc gcatgtcggc
      361 cgtcgagtca tggaccctta tccttgtctc ttcagacaag gacaaaccga actcggacaa
      421 cacggcgcgc ggctcgttga cggcgcgcga gcggtagttg cgcgacttgt accagtcggg
      481 cggaatgccg agcagcatgc gcgggtagca cgagcacagc gtgcagacga tcatgttgtg
      541 gacgtccggc gtgttctcca cgaccaccag cttcagcgcc ggcacgtcca gaccgagctc
      601 ctcgcaggcc tgcgtgccgt tttcgagcag cttcttcctg aacgcatccg agacccaggc
      661 gcgcgcaacc acccgcgccc cgcgttccgg cgaacgctgg cgcatgtcct cgaccgtcgc
      721 ggccacctgc gcctcggtga cgatgccctt ctccaccagc agctcgcgca ggctcacctc
      781 catgagctgg aaataactga agggctggta gctgccgctg tccttcatcc ggcgcgcctc
      841 actcgagcca gtgctggaag atctcgactt ccagcacgtc ctgcgggttg ccgcggtact
      901 gcggccagac gtccttctgc aggaagcgca cgcgatacag cggctgcttc ggctcgccgc
      961 tgcgctgctg cgcgagctcc tcgggattgc cgaaagtgcc gcagcagcgc tcgatcacgc
     1021 cgctgtggcc gcggatgtag aacggcgtgc ggatgtgccc gagcggatac atcctgcgaa
     1081 ccgtcacgcg gtcaccgggc ttgaatcttg tttcgaccat tcttcgcttg gatttccgcc
     1141 atcttccggc cgagctcttc ggtggtgatg accccgcgct ggatcatggt ctgggtgatc
     1201 gaggtcaccc agcgctcgta gtagctcatg cggtcgtagg cgtcgggcgg cagcgcctcg
     1261 atgttcttgc gcagctcgtc cacggtgagc agcttgcggc cgtccttgac gcgggaaagc
     1321 agcatcatca gcgcgtccac gcgccgctcc cactcggcat agtcgtgctc ggtcggctcc
     1381 accctgcccg ccggcaggcc gcccatgtcg tgatggctgc gttcgatcac ggcggttata
     1441 ttagccgcat ggccgtggca gcgcagcaag ttcccccgcc gggcaacctg ttcctcgatc
     1501 acgtttcgca cttcgtgccg gacctgggcg cggcggcgcg cgcgctggag gccctcggct
     1561 tcgcggtcac gccggtgtcg gcgcaacaga cgcaggctgg cccggccggc acgtccaacg
     1621 tctgcgtgat gctcgagcgc ggctacctgg agttcctcgc gccgaccatg gacacgccga
     1681 acgcgcagcg ccttcgcgcc accatgcagc gctacgcggg cgtgcacttg tgctgcttcg
     1741 gcacgccgga cgccgaagga gagcatcgaa ggttgcgggc acatggtttt tcgcccccgc
     1801 ccgtcgtaaa tcttcagcgt gaaacggcgg aagagaaggt ggcgaagttc cacgtcgtga
     1861 gggccgctcc tgaaagaatg cccgaagggc gaatccagtt cgtgcagcac ctcgcgcccg
     1921 aggcgatctg gcggccgcag tgcctcggcc ataccaacaa cgtcgtcaag ctcgcgtgcc
     1981 tgttcgtggt tgccgacgaa accgtggacg ctgcggcgcg ctgggcgcat ttcgcagcgc
     2041 tgctgccgcg gcccgcgggt ggctacgtgc acttgcaggc cgcgcgcggc cacgtgctcg
     2101 tcggcacgcg cgcgcaatgg cgcgagctgc tgggcggcgc gcccgctgcg cctgcgctcg
     2161 ccggctacgc gctcgaatgc cgcgatcccg caatattgat cgcacgctgc g
//
LOCUS       DATDMP010000194         5433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1363459, whole genome shotgun sequence.
ACCESSION   DATDMP010000194 DATDMP010000000
VERSION     DATDMP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363459"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..506)
                     /locus_tag="VI195_03265"
     CDS             complement(90..506)
                     /locus_tag="VI195_03265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6823433.1"
                     /translation="MKEQRTSVAAQAARLPELTAFLQEFWSSASLPPGECLAFELALE
                     EIFMNVVMHGSSGGDLPQVHVSLALTPAGLTLTVEDDGPGFDPLSLPPPDVTASVADR
                     PIGGLGVFLVRNMMDTISYARIAGRNQLRMSKRLES"
     gene            complement(503..865)
                     /locus_tag="VI195_03270"
     CDS             complement(503..865)
                     /locus_tag="VI195_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6823434.1"
                     /translation="MNLRIEAMGEITHVIPEGRLDFDAAPAFQQALERLFTGGGAGPA
                     PTAVVIDGAALEYVSSAGLRAVLLAAKAAQRSGAAFALCALQPAVREVFALSGFGQIM
                     PIHPDLDTALAQLRAPHK"
     gene            complement(862..2046)
                     /locus_tag="VI195_03275"
     CDS             complement(862..2046)
                     /locus_tag="VI195_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY6823435.1"
                     /translation="MSDKAHALAELLLTKRGPCARAFENLILGLIIFSVASIGVESIP
                     DLPQWALQVLRVTEIVVVAVFSFEYLLRLIAATNKLAFIFSYQGLIDLLAIAPFYLAG
                     FDARWLRALRLLRLLRVLKLQTHILESVVEERTRELAAKNAALEEAQAQLKAELEVAR
                     SLQLAILPASFPARAGCDAAARMIPATTMGGDFYDYIELPDGQIGLVIADVSGHGVPA
                     AFFMAVARTNLRELAVRHTDPGECLAQTNDVLCAQNPLDLFVTVFYCILDPKSGVLRY
                     ANGGHNPPYVRRAAGPVESLNGAGGLVLGAMQGVKYPTHALQLLRGDQLVLYTDGVTE
                     AFNPTEELYGAQRLLDEVHVHGSGTPAALVERICKSITNFAGTAPQSDDITLTVLTWG
                     AP"
     gene            complement(2091..3872)
                     /locus_tag="VI195_03280"
     CDS             complement(2091..3872)
                     /locus_tag="VI195_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823436.1"
                     /translation="MSFYPQRRTRALSNESTKDAISPFVVGVTGHRDLRADDLPSLRS
                     AVTEILLRLEKHLPDSQLRIMAGMASGADLLVVQSALDLGVPVDALLPMPLQHYAADF
                     DAETFALLQELLTHPLVRYRELPLASTDRDVHYAALAQHLTRGCSLLIALWDGESSVL
                     PGGTADTVLRYLGVRTDRNKDNERLEFSEASANPDVPVRLVYWIPTARAKSGPLADGG
                     SVEELGAPCYLAGLGDNALQRLPAMPHRLEVHLHSINAYNREYQRFQNRWRAPPAPDS
                     LMRGLPPSMPVDPHDVPVLERIDLQYGKADALALHFQKRSDHLFAFFNLVAFAMGLAY
                     LTYEKFMSTRLLLFAYLLILTSSVGVYHLLHERRWFAKHLMCRALAETLRVKFYLRLA
                     HGDQLVDAEEVLSLSGINRFHGFGWIGHVLIGVEAPPPTGGSPAGVLREGVDLAWIEN
                     QRQYFHRKVAKLHRSGVRTKWLKRSLFGLIIAVVLTLLLLGETAERLVLGIPVENGLT
                     FVVGLVAVILASWELHQNKMASRELLWQYRNQLKHFSRASTQLARTLAAGRRLEILVA
                     LGKDSLMESYLWTIHRFHREHEPPGRA"
     gene            4060..>5433
                     /locus_tag="VI195_03285"
     CDS             4060..>5433
                     /locus_tag="VI195_03285"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY6823437.1"
                     /translation="MDSPKYRAFLSYSHRDTKWATWLHKSLESYRPPKQLIGTVTPRG
                     PVPKRLAPIFRDREELASATDLGSLLNEALRQSACQIVICSPSSARSKWVNEEILAFK
                     RLGREDRIFCLIVGGEPYASYDPAHADEECFPPALRYKLGPDGELSTTRTEPIAADAR
                     PGKDGRNNAKLKLIAGLLAVGFDGLRQREQRRRQRQLFAIASGAIAGMVLTSGLATVA
                     LIARATAQRQTVLAKREAETARQTTAFLVDLFRISDPSEARGNSLTAREVLDKGAARV
                     DVQLAKQPAIQATLQDTLGTVYMGLGLYGQAKPLLETAASKRRTLGAAETPALAVSLS
                     HVGDLLYRRAEYSAAEAAYREAIALQSALPPDQRDDAGLARSLFGLGQELGAQGRSGE
                     AERSFRDALALQKRLFPGPNEDTARTLDELAWTINERDLNEAVPMLQSAVAMQRALWG
                     NQPYPDYA"
BASE COUNT         1023 a         1774 c         1776 g          860 t
ORIGIN      
        1 tgacagtcgg gagcatcaac atgttgatat gcaggcgcaa tcaacattta gagactacat
       61 cgtggctcac cccgcttgcg ggggccagct cagctctcga gccgcttgct catccgcagc
      121 tgattgcgcc cggcaatgcg cgcatagctg atggtgtcca tcatgttccg caccaggaag
      181 acgccgagcc ctcctatcgg ccggtccgcc acacttgccg tcacgtccgg cggcggcagc
      241 gacaacggat cgaaaccggg gccgtcgtcc tcgaccgtca gggtgagacc cgcgggtgtg
      301 agcgcgagcg atacgtgaac ctgcggcagg tcacccccgg acgagccgtg catcacgacg
      361 ttcatgaaga tttcctccag cgcgagctcg aaggccaagc actcaccggg agggagcgat
      421 gctgaagacc agaactcctg cagaaaggcc gtgagctccg gcagacgagc cgcctgggca
      481 gcgacggagg tccgctgctc cttcacttat gcggcgcccg caactgtgcc agggcggtgt
      541 ccaggtccgg gtggatcggc atgatctgac caaacccgga gagcgcgaat acctcgcgca
      601 ccgccggctg cagcgcgcac agcgcaaatg cagcgcccga acgttgggcg gcctttgcgg
      661 ccagcaggac cgcacgtagg ccagcgctcg agacatactc gagcgcggcc ccatcgatga
      721 cgactgccgt gggtgccgga cccgcgccac cgccagtaaa gagacgctcg agcgcctgct
      781 gaaatgccgg cgcggcgtcg aaatcgagcc ttccctcagg gatgacatgc gtgatctcac
      841 ccattgcctc gatccgcagg ttcatggcgc accccaggtg aggaccgtga gagtgatgtc
      901 gtcggactgt ggggcggtgc cggcgaagtt cgtgatgctc ttgcagatac gctcgacgag
      961 tgcggccggg gtaccactgc catgaacgtg cacctcatcg aggagccgct gtgccccata
     1021 gagctcctcg gtcgggttga aggcctcggt cacgccatcc gtgtagagca cgagctgatc
     1081 accccgcagc aactgcagcg catgggtcgg atacttgaca ccctgcattg cgccgagcac
     1141 gagtccgccg gcgccattga gagactccac cgggcctgcg gcgcgccgca cgtagggcgg
     1201 gttgtggcca ccattggcgt agcgcagcac gcctgacttc gggtcgagga tgcagtagaa
     1261 gaccgtcacg aacagatcga gcggattctg ggcgcacaac acatcattgg tctgtgccag
     1321 acattccccg ggatcggtgt ggcgcaccgc cagctcgcgc agattggtgc gtgccaccgc
     1381 catgaagaag gccgccggca cgccgtgccc ggagacatcg gcgattacca gtccgatctg
     1441 accatcgggc agctcgatgt agtcgtagaa atcgcccccc atggtggtcg cgggaatcat
     1501 gcgcgccgcg gcgtcgcatc ccgctctggc gggaaaggaa gccggcagga tggcgagctg
     1561 cagcgatcgc gccacctcga gctcggcttt cagctgcgcc tgcgcctcct ccagtgcggc
     1621 gtttttcgcg gcgagctccc gggtgcgttc ctcgacgacg gactcgagga tgtgcgtctg
     1681 cagtttcagt acgcgcagca gccgcagcag acgcagtgcg cgcagccagc gcgcgtcgaa
     1741 acccgccagg tagaacggcg cgatcgcgag cagatcgatc agtccctggt aactgaagat
     1801 gaaggccagc ttgttcgtcg cagcgatcag gcgcagcaga tactcgaagg aaaatacagc
     1861 gacgaccacg atctccgtca cccgcaagac ctgcagcgcc cactgcggga ggtccggaat
     1921 cgactccacg ccgatggagg cgacgctgaa aatgatcaac ccgaggatga gattctcgaa
     1981 ggcccgggcg caggggccgc gcttggtgag cagcagctcc gccagggcgt gagccttgtc
     2041 actcacggcg ccggccacgc gtcgcaccgg gccgggcacc agagtcaacg tcaggctcgc
     2101 ccgggcggtt cgtgctcgcg gtgaaagcga tggatggtcc acaggtagct ctccatcaac
     2161 gagtccttac cgagcgcgac gagaatctcg agccgccgcc cggcggccag cgtgcgcgcc
     2221 agctgtgtgc tcgcccgcga aaaatgcttc agctgattgc ggtactgcca cagtagctca
     2281 cggctggcca tcttattctg gtgcagctcc caggatgcca gtatcacagc gacgagcccc
     2341 accacaaagg tcaggccgtt ttcaactggt atacccaaaa ccagccgctc cgccgtctcg
     2401 ccgagcagca acagagtcag caccacggcg ataatgaggc cgaacaggct gcgcttcagc
     2461 cacttggtac gcacgccgct gcggtgcagt ttagccactt tcctgtgaaa gtactggcgt
     2521 tgattctcga tccaggcgag atcgaccccc tcgcgcagga cgccggccgg tgagccgccc
     2581 gttggcggcg gtgcctcgac cccgatcaat acatggccga tccagccgaa accatggaag
     2641 cggtttatcc ccgagagcga gagaacctct tcggcgtcga ccagctggtc gccatgggcg
     2701 agccgcaggt agaacttcac gcgcagcgtc tcggccagtg cacggcacat cagatgcttg
     2761 gcgaaccagc ggcgctcatg cagcaggtga tacacgccca cgctcgaggt gaggatgagc
     2821 agatacgcga acagcagcag ccgggtactc atgaatttct cgtacgtgag gtacgcgaga
     2881 cccatcgcga aggcgaccag gttgaaaaat gcgaacaggt ggtccgagcg cttttggaag
     2941 tgcagcgcga gcgcgtcggc cttgccgtac tgcaggtcga tgcgctccag caccggcaca
     3001 tcgtgtgggt cgaccggcat agacggcggc agacccctca tcagggagtc cggggccggg
     3061 ggagcgcgcc agcggttctg gaagcgctgg tattcgcggt tataggcatt gatggagtgg
     3121 agatgtacct cgaggcggtg cggcatcgcg ggcaagcgct gcagcgcgtt atcgccgagt
     3181 ccggcgagat agcagggcgc tccgagctcc tctaccgagc ccccatccgc tagcgggcca
     3241 ctcttcgcgc gcgcggtggg aatccagtac accagccgca ccggcacgtc cggatttgcc
     3301 gacgcttccg agaactccag ccgctcattg tccttgttgc ggtccgtgcg cacaccgagg
     3361 taacgcagca ccgtgtcggc agtgccgccg ggcagcaccg atgactcacc gtcccagagg
     3421 gcaatcagca ggctgcagcc gcgggtgaga tgctgcgcga gcgcggcgta gtggacgtca
     3481 cggtcggtgc tggcgagcgg cagctcgcgg tatcggacca gcggatgcgt cagcagctcc
     3541 tgcaacagcg cgaaagtctc ggcatcgaag tccgcagcgt agtgctggag cggcatcggc
     3601 agcagcgcat cgacggggac tccgaggtcg agagcgctct gcacgacgag cagatcggcg
     3661 cccgaggcca tgccagccat gatgcgcagc tgggagtccg gtaggtgctt ctcgagccgc
     3721 agaaggattt ccgtgaccgc agaacgcaaa gacggcaagt catccgcacg cagatcgcgg
     3781 tgcccggtaa ccccaacgac gaaaggcgag atagcgtctt tcgtgctctc gttcgacaaa
     3841 gctctcgtgc gccgctgggg ataaaaggac attcgctgcg ggcgattgtg ccgcgtgcag
     3901 gcgacaccgg acaacggcac ggcccgccta ccaccggcgg ctctcgcgcg gctggccgcc
     3961 agtgcgctag actcggtcaa ttccgtatac ccctccggac cgcgaaaaga ccgaggtagc
     4021 cgcgccagaa gggtgactct ccgacgagga cattgcctct tggattcccc caagtaccgg
     4081 gcgttcttga gctacagcca ccgcgacacc aagtgggcca cgtggctgca taagtcgctc
     4141 gagtcctatc ggcctcccaa gcagctcatc ggcacggtaa ccccgcgcgg cccagtgcca
     4201 aagcgtctcg caccgatctt ccgggaccgc gaggagcttg ccagcgccac tgatcttggg
     4261 tcgctactga atgaggccct gaggcagtcc gcctgccaga tcgtcatctg ctcaccgagc
     4321 tcagctcgct cgaagtgggt gaacgaggag atcctggcct tcaagcgact cggtcgcgaa
     4381 gaccgcatct tctgcctgat cgtcggtggc gagccctacg catcgtatga tcccgcgcac
     4441 gctgacgagg agtgttttcc gcccgcgctt cgttacaagc tcggacccga tggcgagctc
     4501 agcaccacgc gtaccgagcc aatcgccgcg gacgcgcggc ccggcaagga cggccgcaat
     4561 aacgcgaagc tgaagctgat tgcgggcctt ctggccgtgg ggttcgacgg cctacgccag
     4621 cgggagcagc gtcgccggca gcggcagctc tttgcgatcg cctccggagc catcgccggc
     4681 atggtgctga cgagcgggct cgccaccgtc gccctgattg cccgcgccac ggctcagcgg
     4741 cagacggtcc ttgccaagcg cgaagcggag accgcgcgcc aaacgaccgc atttctcgtt
     4801 gacctgttcc ggatttcgga cccgagcgag gcccgcggca atagcctcac cgcgcgcgag
     4861 gtgctggaca agggcgcggc gcgggtggat gtgcagcttg ccaagcagcc tgcgatccag
     4921 gcgacgctgc aagacaccct cggaaccgtg tacatgggcc tgggactgta cggccaggcg
     4981 aagccgctgc tggaaaccgc ggcgagcaag cggcggacgc tcggcgcagc ggagactccc
     5041 gcgctggccg tgtccttgag tcacgtcggg gacctgctct accggcgtgc ggagtattcc
     5101 gcggccgaag ccgcatatcg cgaggccatc gcgctgcaga gcgcattgcc accggatcag
     5161 cgtgacgatg cgggtcttgc ccggagtctt ttcggcctgg gccaggagct cggtgcgcaa
     5221 ggacgctccg gggaagccga gcggagtttc cgggatgcgc tcgcgctgca gaagcgcctc
     5281 tttcccggcc ccaacgagga caccgcgcgc accctcgatg agctcgcctg gacgatcaac
     5341 gagcgcgatc tgaatgaggc cgtcccgatg ctgcagagcg ccgtggctat gcagcgtgcg
     5401 ctgtggggca accagcctta cccggattac gcg
//
LOCUS       DATDMP010000195         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_30626, whole genome shotgun sequence.
ACCESSION   DATDMP010000195 DATDMP010000000
VERSION     DATDMP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30626"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VI195_03290"
     CDS             complement(<1..1083)
                     /locus_tag="VI195_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6823438.1"
                     /translation="MATVQPIVEDSSSFVQREYSPAAKRALARKPALFINNEWVSSTH
                     GATLVVEDPSTGREVGRIVDASDADVDRAVAAARAAFDDGRWSNLPPKARERALNKLA
                     DLIEANAAEFAELEAIDNGKPVGFAKMIDVPGSVDHLRYMAGWASKIGGEVVDPLGMP
                     RGKVFSYVSRQPVGVAVQIIPWNFPLIMATLKISPALAAGCTLVLKPAEQTSITALRL
                     ADFVAEAGFPPGVINVITGNGHTAGDRLVKHPDVDKVAFTGSTEVGKIINRNATETLK
                     RVTLELGGKSPVIVMPDVNVDEVAPAAAGGVFFNAGQICVAGSRLFAHRSIFDRMVEG
                     VTKAAAFWRPRPSLDPEGHMGPLVSKE"
     gene            complement(1232..2065)
                     /locus_tag="VI195_03295"
     CDS             complement(1232..2065)
                     /locus_tag="VI195_03295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6823439.1"
                     /translation="MTEAKDYPLGYSEQEARRLAEQGALLEEFTAELFQRAGLRQRMR
                     VLDIGCGVGDVALLAARVVGPQGAVLGIDRAASSVETASARASALGVGHVRFEQAELT
                     NFGTGQTFDALVGRLVLLYLPDPAETLGRLSRHLRPDGIIAFQEFDMSATSQVPASEL
                     FLEVRGWLLSAFAAAGAQLDMGTKLYSCFLRAGLPPPQMTAATPVACGPATGGYGYLV
                     AVLRSLLPLIERTGIATAAQIGIDTLAARMRDDAIAHQRVLFLPRVVGAWTALDQHPT
                     A"
     gene            2242..2937
                     /locus_tag="VI195_03300"
     CDS             2242..2937
                     /locus_tag="VI195_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823440.1"
                     /translation="MLVHAYRHARLMEESELRVQMSIYLHTPADAIARRMLDCPDTYH
                     RWEVEHGRLMHTISDRGRLPEQITALRTTVMALVHRRAVFEYLREGQITGRKRRRVLA
                     LFYGCRDYTNALLREHGNYVRCSSSYLCTHHLGEHLMQDAAFNEPLQLYEEWFGEYFR
                     AYCDTELAETEHEKNAVVALDAVKPLLKYRLAEARQAILVMPQSPDREWREVEIRKPN
                     GDTQRMRAIFGRN"
     gene            complement(2969..3466)
                     /locus_tag="VI195_03305"
     CDS             complement(2969..3466)
                     /locus_tag="VI195_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823441.1"
                     /translation="MEARKLRHADSEPFQLSLRIRHPSLDPEELSRELNIEAAHCFRA
                     GEPRASRSGIASVHPESYWLGALNTAHWPVDASFAGVQPLTASEERFGVAAPRSLGWV
                     LSLSTRFFSAHAATLRRICAEGGQMSLLITAPAGGVSGFSLAPAVSRVFSELGITLEF
                     EFTTD"
     gene            complement(3715..>3946)
                     /locus_tag="VI195_03310"
     CDS             complement(3715..>3946)
                     /locus_tag="VI195_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823442.1"
                     /translation="TGRVVHDERGNAMWDWLKQTGRNAIESTTRMLRKLESPELKVEQ
                     SHEEELRIQPDGNCAGGGYDPYNQPTKSRRTK"
BASE COUNT          672 a         1274 c         1326 g          674 t
ORIGIN      
        1 ttccttcgag accagcggac ccatgtggcc ctcgggatcg agtgagggcc gcggccgcca
       61 gaaggcggcg gccttcgtca ccccttcgac catgcgatcg aagatcgagc ggtgtgcgaa
      121 caggcgcgag cccgcgacac agatctgccc ggcgttgaag aacacgcccc cggcagcggc
      181 gggggcgact tcgtcgacat tgacgtcggg catcacgatg accggtgatt tgccgccgag
      241 ctcgagggtg acccgcttga gggtctcggt ggcgttgcgg ttgatgatct tgccgacctc
      301 ggtggagccg gtgaaggcca ccttgtcgac gtccgggtgc ttcaccaggc gatcgcctgc
      361 ggtgtgtccg ttgccggtga tgacgttgat cacaccggga gggaagcctg cctcggcgac
      421 gaagtccgcc aagcgcagcg cagtgatcga ggtctgctcg gcgggcttga gcaccagcgt
      481 gcagccggcg gcgagcgccg gcgagatctt cagcgtcgcc atgatgagcg ggaagttcca
      541 cgggatgatc tgaactgcca ccccgaccgg ctggcgcgag acgtagctga aaaccttgcc
      601 ccgcggcatt cccagaggat cgacaacctc accgccgatc ttggaggccc agcctgccat
      661 gtagcgcagg tgatccacgg aaccggggac atcgatcatc ttcgcaaagc cgaccggctt
      721 gccgttgtcg atggcctcga gctcggcaaa ctccgcggca ttcgcctcga tcaggtccgc
      781 gagcttattg agtgcgcgct cgcgcgcctt gggcggcaga ttcgaccaac gtccgtcatc
      841 gaacgcggcg cgtgctgccg caacggcgcg gtcgacgtcc gcatcggaag catccacgat
      901 acggccgact tcccgtccgg tggagggatc ctcgacgacg agtgtcgcgc cgtgcgtcga
      961 tgacacccat tcgttgttga tgaagagcgc cggcttgcgc gcgagggcac gcttggcggc
     1021 tggcgagtac tcgcgctgca cgaaggagct gctgtcctcg acgatcggtt gcacggtggc
     1081 catgtcacta tcctctgggg ttgcccgggc gcggccgcgc cgccccatct ggctgatttt
     1141 gtttgtgtag cgtactaagt gaccccgggg agggtcaatg caagagggcg cggagcgcca
     1201 gctcgcgctg tgcggcgaga gtgaccgcaa atcaagcagt tgggtgttgg tccagcgcgg
     1261 tccaggcgcc taccacgcgc ggcagaaaca gcacccgctg gtgcgcgatc gcgtcatcgc
     1321 gcatccgcgc tgcgagcgtg tcgatgccga tctgcgccgc ggtggcaata ccggtccgct
     1381 cgatcagggg caggaggctg cgcaggacgg caaccagata cccatagccc cccgtcgccg
     1441 gtccgcacgc gaccggcgtc gcggctgtca tttggggagg aggcagtccc gcgcgcagaa
     1501 agcacgaata gagcttcgta cccatgtcca gctgtgcgcc tgcggcggcg aaggcggaca
     1561 ggagccatcc tcgcacctcg aggaacagct cgctggccgg cacctgcgaa gtcgccgaca
     1621 tatcgaattc ctgaaacgcg atgatcccgt ccgggcgcag gtgtcgcgac aggcggccga
     1681 gcgtctctgc gggatccggg aggtagagca gcaccagccg gccgacgagc gcatcgaagg
     1741 tctgcccggt gccgaaattg gtgagttcgg cctgctcgaa gcgcacgtgg cctacgccga
     1801 gggcgctggc gcgggcgctg gcggtctcca ccgaggatgc ggcgcggtcg atgcctagca
     1861 cggcaccttg cggacccacg actcgggcgg cgagtagcgc cacatcgccc accccgcagc
     1921 cgatgtcgag cacccgcatc ctctggcgca gccccgcgcg ctgaaacagc tccgcggtga
     1981 actcctcgag cagcgccccc tgctccgcca gacggcgcgc ttcctgctcg gagtacccga
     2041 gcggatagtc ttttgcctcg gtcatggtgg ctccccacgg tctcggcaag cgtaacgcag
     2101 ccgctctcgc gggctgaaac acgaccttca acgtaacaaa taccgtgctg agtattgcgc
     2161 tgcaacgcgc gagcgcggag acttgcgagt ggtcgtatcg agaagagtcg gtcggggagt
     2221 tgaaccagtc tctgagggcg catgctcgtc catgcgtata ggcatgcacg gctcatggag
     2281 gagtcggagt tgcgcgtgca gatgtccatt tacctgcaca cgcccgctga tgccattgcg
     2341 cgccgcatgc tcgattgtcc ggacacctac catcggtggg aggtcgagca cggccgcctg
     2401 atgcacacca tttccgaccg tgggcggctc ccggagcaga tcacggcgct gcgaaccacg
     2461 gtaatggccc tcgtgcaccg gcgtgcggtg ttcgagtatc tgcgcgaggg acagatcact
     2521 ggacggaagc gccgccgcgt cctggcgctt ttttacggtt gccgtgacta caccaatgcc
     2581 ttgctccgcg agcacggcaa ctacgtgcgc tgcagctcga gctatctgtg cacccatcac
     2641 ctcggcgagc acctcatgca ggatgccgcc ttcaacgagc cgctgcagct gtacgaggag
     2701 tggttcggag agtacttccg tgcctattgc gataccgagc tcgccgagac agagcacgag
     2761 aaaaatgcgg tcgtggcgct cgatgccgtg aagccgctcc tcaagtaccg cctggccgag
     2821 gcgcggcagg cgattctggt catgccgcag tcaccggatc gcgagtggcg cgaggtcgag
     2881 atccgcaagc cgaacggcga cacgcagcgg atgcgggcga tcttcggccg caactgaccc
     2941 gcatgcctcg gcggaggcgc gagtcctttc agtcggtcgt gaactcgaac tcgagcgtga
     3001 tgccgagctc gctgaatacc cggctcactg ccggggcgag actgaagccg ctcacaccgc
     3061 ccgcgggtgc cgtaatcagc aggctcatct gcccgccttc cgcacagatg cgccgcagcg
     3121 tggcggcgtg tgcactgaag aagcgcgtgg agagcgacag cacccatccg agactccgcg
     3181 gcgccgccac accgaagcgc tcctcgctgg ccgtcaaagg ctgcactccg gcgaaggagg
     3241 catcgacggg ccaatgagcc gtattgagtg cgccgagcca gtagctctcg gggtgtacgg
     3301 aggcgatgcc gctgcgcgag gcacgtggct ctcccgcccg aaagcagtgc gccgcctcga
     3361 tattcagctc gcgagaaagc tcctcgggat cgagcgaggg gtgacggatc cttagggaaa
     3421 gctggaacgg ttcggaatcg gcgtggcgga gctttctggc ttccatgaac cttcctctgt
     3481 tcgcagcgct gcagcgcgcg cctgcgactg gcagtcgacg tcactgcgta gagagcctcg
     3541 gggtacgtcc gtggtacgcc ccgtggcccg gggccgtaag actaaatcac ccgcagccgc
     3601 catgcacgct gaatgtggcc cgcgtaccac gggtcaccgc atctcgatgt agccatacgg
     3661 cgacgtgata tgtggtggcg cgcgagtgtg ggcgcctgag agcggcgcgc cggcttattt
     3721 cgtgcgacgg gacttggtgg gctggttata ggggtcgtaa ccgccgccgg cgcagttgcc
     3781 gtccggctgg atgcgcagct cctcttcgtg cgactgctcg actttgagct ccggagactc
     3841 gagctttctc agcatccgcg tggtgctctc gatggcgttg cggccggtct gcttcagcca
     3901 atcccacatt gcattgccgc gctcatcgtg aaccactctg ccggtc
//
LOCUS       DATDMP010000196         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_141316, whole genome shotgun sequence.
ACCESSION   DATDMP010000196 DATDMP010000000
VERSION     DATDMP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141316"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VI195_03315"
     CDS             <1..615
                     /locus_tag="VI195_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6823443.1"
                     /translation="LIALPLGVLQQRGSAPGAVCFSPALEMKRAALAGLASGPVIKLQ
                     LLFASAFWETVRGGRYRDAGFFHAPAAEFPTFWTPAPARAPLLVAWVGGPRAQRLAAD
                     AASQGIVRKALASLKALFGEIDVHAELRAWYYHDWQQDPYARGAYSYVTVGAGVARAA
                     LAQPLEDTLFFAGEATDVEGGTVAGALRSGQRAAREVLAADGPQ"
     gene            complement(625..1821)
                     /locus_tag="VI195_03320"
     CDS             complement(625..1821)
                     /locus_tag="VI195_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823444.1"
                     /translation="MHRPRAALIRIDRGPRPARTASAALTALLILASFAGCSLVSIKS
                     PEKPLSTRDMNARILTRELTTHFLEANTRSADLILASEADPVVIEHTLRWELGVIDTS
                     RQAETQLAPLMSLLDTWALALQLQAFVSAGAPGAKLFGTHQPVLRAITDKYADDVTSL
                     AHTLLTARELTEYQSFVTGYVRDHPLQDLRFARPSVPTEWSRAKGGESSLLDEVGTIP
                     QALADTAQRLQIYGDTVPQQAVRRSQLALRESGYTPSDLRTALARLDERLDRLTAVAE
                     GSPELVRDAEAQLRESLRELMHQLDSSVRATTAALHSEREALFTEIQEEREAVLAAVD
                     VQRKALSADAGRIGDQLVRTSGAEVRRFTREALLLIIALSVVLLGLPFAAGYLVGRAR
                     SSPRPT"
     gene            complement(1840..>2624)
                     /locus_tag="VI195_03325"
     CDS             complement(1840..>2624)
                     /locus_tag="VI195_03325"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEY6823445.1"
                     /translation="LELAIRFGPRLFTALLIIIVGFLVSGWVSRWFSQLLGRRDLEPP
                     LRLLLSRIVWALCVLLFALLALQNLGVELLPLIAGLGVAGAGLALATQGVLSNIVAGL
                     SIIFSKPFRVGEYIAIAGVEGVVESITLFNTTLGHVDLSRVVVPNRKVVGEILHNYGR
                     IRQVEVSIGVAYDSDIAAVLELIHAALQANPRVLREPQPVVQPIQFADSAVSIAVRPW
                     VAVGDQVAASGEIHAAVLAALRARGVVIPPPQREVRLIGATA"
BASE COUNT          421 a          906 c          915 g          382 t
ORIGIN      
        1 ctcatcgcac tgccgctcgg ggtgttgcag cagcgcggga gcgcacccgg ggcggtgtgc
       61 ttcagcccgg cgctcgagat gaaacgcgcc gcgctcgcgg gcctcgcctc agggccggtc
      121 atcaagctgc agctgctgtt cgcgagtgcc ttctgggaga cggtacgcgg gggccgctac
      181 cgggatgccg gattctttca cgcacccgcg gccgagttcc cgaccttctg gacgccggca
      241 cccgcacgcg cgccgctgct ggtcgcctgg gtgggcggtc cgcgcgcgca gcgcctcgcg
      301 gccgacgccg cgtcacaggg catcgtgcgc aaagcactcg cgagcctgaa ggcgctgttc
      361 ggcgagatcg acgtccatgc tgagctgcgc gcctggtact accacgactg gcagcaggat
      421 ccttacgcgc ggggagccta tagctacgtc accgtcggcg cgggcgttgc acgcgcggcg
      481 ctcgcgcagc cgctcgagga cactctgttc ttcgccggcg aagcgaccga cgttgagggt
      541 ggcacggtcg cgggcgcgct gcgcagcggt caacgcgccg cccgcgaggt actcgcggcg
      601 gatggcccgc agtaacgcgc cgcgctacgt gggccgcggt gacgagcgag cgcggccgac
      661 gagataaccg gcggcgaagg gtaagcccaa cagcaccacg ctgagcgcga tgatgagcag
      721 cagcgcttcg cgcgtgaaac gccgcacttc ggcgccggaa gtgcgtacca gctggtcgcc
      781 gatcctcccg gcatcggcgc tgagcgcctt gcgctgcacg tccaccgcgg cgagcacggc
      841 ttcccgctcc tcctggatct ccgtgaacag cgcctcacgc tcgctatgca gtgcggcggt
      901 cgtcgcgcgc acggatgagt ccagctggtg catcaactcc cgcagtgact cccgcagctg
      961 cgcttccgca tcgcgcacca gctcgggtga gccctccgca acggccgtca gccggtcgag
     1021 ccgctcatcg agccgcgcca gcgctgttcg caggtcgctc ggtgtgtatc ccgactcccg
     1081 cagcgccagc tgcgagcggc gcacggcctg ctgcggcacg gtgtcgccgt agatctgcag
     1141 ccgctgagcc gtgtcggcga gcgcctgcgg tatggtgccg acctcatcga gcaggctcga
     1201 ctccccgccc ttcgcacggc tccactcggt gggcaccgaa ggacgcgcga accgcaggtc
     1261 ctgcagcgga tggtcacgca cgtagccggt gacgaagctc tgatactcgg tgagctcgcg
     1321 ggcagtgagc aaagtgtgcg cgaggctcgt tacatcgtcc gcgtacttgt cggtaattgc
     1381 gcgcagcacc ggctggtgcg tgccgaagag tttcgcgccg ggtgcgccag cgcttacgaa
     1441 ggcctgcagc tgcagcgcca gtgcccaggt atcgagcaag ctcatgagag gcgcgagttg
     1501 cgtctcggcc tggcggctcg tgtcgatcac gccgagctcc cagcgcagtg tgtgctcgat
     1561 caccaccgga tcggcctcac tcgccaggat gaggtcggcg ctgcgggtat tcgcctcgag
     1621 gaagtgcgtc gtcagctcgc gcgtgaggat gcgcgcattc atgtcccgcg tggacaaggg
     1681 tttttcggga ctcttgatgg aaacgagcga gcagcccgcg aagctcgcaa ggatcagcag
     1741 cgcggtgagc gccgcgctcg cggtgcgggc gggccgcggg ccgcggtcga tgcggatgag
     1801 agcggcgcgc ggtcggtgca tggcgaggcc ggcgcccgct caggccgtcg cgccgatgag
     1861 ccgcacctcg cgctgcgggg gcggtatcac gaccccacgg gcacgcagcg ccgcgagcac
     1921 cgcggcatgg atctcaccgc tcgccgcgac ctggtccccc accgcaaccc aggggcgcac
     1981 ggcgatgctg accgcggaat ccgcaaactg tatcggctgc acgaccggct gcggctcgcg
     2041 cagcacccgc gggttggcct gcagtgccgc gtggatcagc tcgaggacgg ccgcgatgtc
     2101 gctgtcgtag gccaccccga tgctgacctc gacctggcgg atgcggccgt agttatggag
     2161 gatctcgccc accaccttgc ggttgggaac caccacccgc gagagatcga cgtgcccgag
     2221 cgtcgtgttg aagagcgtga tcgattcaac cactccttcc accccggcga tggcgatgta
     2281 ttcgccgacg cggaacggct tggaaaagat gattgagagt cccgccacga tgttgctgag
     2341 caccccctgg gtggcgagcg cgagccccgc accggcgaca ccgagccccg cgatgagcgg
     2401 cagcagctcg acgccgagat tctgcagcgc gagcagcgca aagagcagca cgcagagcgc
     2461 ccagacgata cggctgagca gcaaccgcag cgggggctcg aggtcgcgcc gcccgagcag
     2521 ctgggaaaac cagcgactca cccagccgct caccaaaaac ccgacgatga tgatcagcag
     2581 cgcggtaaac aggcgcggac cgaaacgtat cgccagctcg agga
//
LOCUS       DATDMP010000197         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_586046, whole genome shotgun sequence.
ACCESSION   DATDMP010000197 DATDMP010000000
VERSION     DATDMP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586046"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VI195_03330"
     CDS             <1..447
                     /locus_tag="VI195_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY6823446.1"
                     /translation="LGTSGMAGGQAMDVAVLGRQVTVAEVEAMYARKTGALIRASVLM
                     GAACAPALAAELWEALAAFASSIGLAFQIQDDLLDELADTATVGKATRADRARGKPTY
                     PAVLGVAASQERVRRLHAQALGALAPFGPSADALRALADWLLVRSY"
     gene            complement(444..2333)
                     /gene="dxs"
                     /locus_tag="VI195_03335"
     CDS             complement(444..2333)
                     /gene="dxs"
                     /locus_tag="VI195_03335"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY6823447.1"
                     /translation="MASFPLLSGLESPADLRRLPLAQLPTLARELRTFLVHNAATCAG
                     HFAAGLGTVELAIAVHYVFDTPTDRVVWDGGHQTYPHKVLTGRCRDLPTIRQPGGLQP
                     FPQRAESEYDAFGVGHLSTSISAALGMAVAAARRGEPRRVVAIIGDKALTGGMAFEAL
                     NHAGSLKSDLLVILEDSDAHSDAGGLLSSDFARVLSGWVYTQLREGGKKALRQMPTVR
                     ELARRSEQHLKGMVLPGTLFEELGVNYIGPLDGHDIRALVATLRNLQRRHGPQLLHVV
                     TRKGKGHTATATGPIQRPVSCDSSAAPPLNTRSRGGTASLSYSQVFGQWLCDMAEAEP
                     GIVGVTPATLEDSGLLEFAKRFPDRCFDLSVAHQHAVTFAAGLATEGLKPVVAIPSTY
                     LQRTYDQLIHDVALQRLPVLFAIDRAGLVGGDGASHQGSYDLSYLRCIPNLTIMAPAD
                     ENECRQMLYTASTLFGPVLVRYPRGNGPGVSVDAAMSALAVGRAQECREGHSRLALLV
                     FGAPLAAARAVAERLDATLVNMRFVKPLDVDLLTALSAHHRAFVTIEENAIAAGAGSA
                     VAEALVSRDVRVPLLQLGIPDRFIEHGSRESCLAAARLDATGLSDSIERWWTLHQPMR
                     SAAGG"
     gene            2475..>2807
                     /locus_tag="VI195_03340"
     CDS             2475..>2807
                     /locus_tag="VI195_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY6823448.1"
                     /translation="MDFGNPDISYQERILPRVSRTFALTIPQLPARLRTAVSNAYLLC
                     RIADTIEDEPALSAAETRGFLQRFTAVVFGTEDAAPLARELAGRLSDRTLPAERELVG
                     NMERVVRVT"
BASE COUNT          465 a          933 c          948 g          461 t
ORIGIN      
        1 ctcggcacga gcggcatggc cggcggtcag gcgatggacg tcgccgtgct gggacggcaa
       61 gtaactgttg ccgaggttga ggccatgtat gctcgcaaga ccggagcact gatccgggcg
      121 agtgtgctga tgggcgcggc ctgcgcaccg gcgctcgccg ccgagctgtg ggaggcgctg
      181 gctgcctttg cctcctcaat cgggcttgcg tttcagatcc aggacgattt gctcgatgag
      241 ttggcagaca cggcgacagt gggcaaggcg acccgcgccg accgtgcgcg aggcaaaccc
      301 acttatccag cagtcctcgg cgtggcggcc tcacaggagc gggttcggcg gctgcatgcc
      361 caggcactcg gcgcgctggc gccgttcggc ccgtctgccg atgcgctgcg tgccttggcc
      421 gattggctgc tggtacgcag ctattagccg ccggctgcgg agcgcattgg ctggtgcaac
      481 gtccaccaac gctcgatact gtcgctcaga cccgtcgcgt ccaggcgcgc agccgccagg
      541 cagctctcgc gcgagccatg ctcgatgaag cggtccggaa tgccaagctg cagcaacggg
      601 acgcgtacat cgcgagacac cagcgcctcg gcgaccgctg agccggcgcc ggccgcgatg
      661 gcgttctcct cgatcgtgac gaatgcccgg tggtgcgcgc tgagggcggt caacaggtcc
      721 acatcgagcg gctttacgaa acgcatgttc acgagcgtgg cgtcgaggcg ttcggcgaca
      781 gcgcgtgccg cggccagcgg tgcgccgaac accagcaggg caaggcggct gtggccttca
      841 cggcattctt gcgcgcggcc aaccgccagg gccgacattg cggcgtcgac ggagactcct
      901 ggcccgttgc cgcgcggata gcggaccaac accgggccga acagcgtgct cgcggtgtag
      961 agcatctggc ggcactcatt ctcgtccgcc ggcgccatga tggtcaggtt cggtatgcag
     1021 cgcagatagg acagatcgta gcttccctga tgacttgccc cgtcgccgcc caccaggccc
     1081 gcgcggtcga tggcgaacag cacaggaagc ctctgcagcg ccacgtcgtg gatgagctga
     1141 tcataggtgc gctgcaggta cgtcgaaggg atcgccacca ccggcttcag gccctcggtc
     1201 gcaaggccgg cggcaaaggt caccgcgtgc tggtgagcga cgctcagatc gaagcaacgg
     1261 tccgggaatc tcttcgcgaa ctcgagcagg cccgagtcct cgagtgtggc aggggtgacg
     1321 ccgacgattc ccggttccgc ctccgccata tcgcacagcc actgcccaaa cacctggctg
     1381 tagctcagac tggcggtacc tcccctcgat cgtgtgttca gcggcggtgc ggcgctcgag
     1441 tcgcaggaca ccgggcgctg gatcggaccg gttgccgtgg ccgtgtgacc cttgcccttg
     1501 cgcgtcacga cgtgcagaag ctgcgggccg tgccgcctct gcagattgcg cagcgtcgca
     1561 acgagtgccc ggatgtcgtg gccgtccagc ggaccgatgt agttgactcc cagttcctcg
     1621 aatagcgtgc ccggcagcac catgcccttc aggtgctgtt cggagcggcg tgccaactcc
     1681 cgcaccgtgg gcatctggcg cagcgccttc ttgccgccct cccgcagctg cgtgtacacc
     1741 cagccggaga gtacgcgtgc gaagtcactt gacaggagtc cgccagcatc agagtgagcg
     1801 tcactatctt cgagaatgac cagaaggtcc gacttgagtg aaccggcatg gttgagcgcc
     1861 tcgaaagcca tgccgccggt caaggcctta tccccgatga tcgctacgac ccgccgtggc
     1921 tccccgcggc gggccgccgc gacagccatg ccaagcgcgg cgctgatgga agtgctcaag
     1981 tgtccgacgc cgaaggcgtc gtactcgctc tcggcccgct gtgggaacgg ctgcaggccg
     2041 ccgggctgcc tgatggtggg caggtctcta caccgcccgg tcagcacctt gtgcgggtag
     2101 gtctgatgac caccgtccca caccacccgg tcggtcgggg tatcgaacac gtagtgaacg
     2161 gctatggcca actccacggt tccaaggcct gccgcaaagt gcccggcgca cgtcgcagcg
     2221 ttgtgaacca gaaaggtgcg cagctccctg gcgagggtcg ggagctgcgc gagcggcagg
     2281 cggcgcaggt cggccgggga ctcgagcccg ctcagaaggg ggaaggaggc cacgtccatc
     2341 atcgactgca agtctacacc gcggctccgg caaggcttgc ctgagcttta gcgttgcgca
     2401 agactatatt tccggccgcg cccacgcggc gcgcggcgta cgccgcattg gcacaacggt
     2461 gcgagagctt ccaaatggac ttcggaaatc ctgacatcag ctaccaggag cggattcttc
     2521 cgcgtgtctc gcgcaccttc gcgttgacca tcccgcagct gccagcgcga ctgcgcacgg
     2581 cggtcagcaa cgcgtacctc ctgtgccgca tcgctgacac catcgaggac gagccggcgc
     2641 tgtcggcggc cgagacccgg ggtttcctgc agcgtttcac ggcggtcgtg tttggcaccg
     2701 aagatgcggc accgctcgca cgcgagctcg ccgggcggct ctcggatcga accctgcccg
     2761 cagagcgcga gctggtaggc aatatggagc gagtcgtccg cgtgacc
//
LOCUS       DATDMP010000198         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1585784, whole genome shotgun sequence.
ACCESSION   DATDMP010000198 DATDMP010000000
VERSION     DATDMP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585784"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VI195_03345"
     CDS             <1..370
                     /locus_tag="VI195_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6823449.1"
                     /translation="GLAAAMDYLEGLGIEAVAAHEQRLLELATRELARMPQIELIGTA
                     AHKAAVLSFTMKGVHAHDLGTILDADGVAVRTGHHCAMPVMTFFGLPATARASFACYN
                     TEEDVERLVRALERAGEVFA"
     gene            370..813
                     /locus_tag="VI195_03350"
     CDS             370..813
                     /locus_tag="VI195_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY6823450.1"
                     /translation="MDLKELYRDVILDHNRRPRNFGALAGASHHAEGNNPLCGDRLSL
                     ALRLDGDRIADIRFEGSGCAISTASASLMTEAVKGKDRAAVRQLFEEVHTLLTQPDAP
                     ADATLGKLAALSGVREFPARVKCASLCWHTLNAALSSSAAPVSTE"
     gene            818..1369
                     /gene="sufT"
                     /locus_tag="VI195_03355"
     CDS             818..1369
                     /gene="sufT"
                     /locus_tag="VI195_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEY6823451.1"
                     /translation="MRRYENEPFVVSREVRAVIVPAGQEVKLKPGQAGYITQALGGSF
                     TVYLEGNLFRIAGEDADAIGQEPVKPPELPPNASEEDVKGLAWEQMRTCYDPEIPINI
                     VDLGLVYDCAVTQNEDGTRTVDVTLTLTAPGCGMGDILVDDVRDKISRIPTVREARVS
                     LTFDPPWNQSMMSEAARLQTGMF"
     gene            1392..1706
                     /locus_tag="VI195_03360"
     CDS             1392..1706
                     /locus_tag="VI195_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY6823452.1"
                     /translation="MSWQDVGPAAAVRESAPLSVEVEGVHLAIVRCGDALYAVEDRCT
                     HDGESLGEAQVEDCQIICPRHGAHFCLRTGEALTPPAYEPLRTYRVREQDGRLLIENP
                     SE"
     gene            1714..2229
                     /locus_tag="VI195_03365"
     CDS             1714..2229
                     /locus_tag="VI195_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY6823453.1"
                     /translation="MKRLTLMRHADARWQDARMSDLERPLNRRGTAAAEAMARRLLEL
                     ALVPQLLLVSPARRTQQTGEIVAGVLALPARLVISDEGLYLASAADLLKVVQATGPRV
                     AHLLVVGHNPGVSELVQQLVPEAEASGLATAALCSIAFECDQWPAVGVAAVKDVMREA
                     PPSRRLFWLFG"
     gene            complement(2259..>2526)
                     /locus_tag="VI195_03370"
     CDS             complement(2259..>2526)
                     /locus_tag="VI195_03370"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEY6823454.1"
                     /translation="NAAEIGMEHHLGMTCDPVAGLVQIPCIERNAMGAVKAVNAARLA
                     MRGDGSHKVSLDQVIATMRQTGADMSTIYKETSQGGLAVNVPEC"
BASE COUNT          395 a          859 c          877 g          395 t
ORIGIN      
        1 cgggctcgcc gccgccatgg actacctcga ggggctcggc atcgaggcag ttgccgccca
       61 cgagcaacgg ctgctcgagc tcgcgacccg cgagctcgcg cgcatgccgc agatcgagct
      121 catcggcacc gccgcccaca aggccgcggt gctctccttc accatgaaag gcgtgcatgc
      181 gcacgatctc ggcaccatcc tcgacgccga cggtgtggcg gtgcgcaccg gccaccactg
      241 cgccatgccg gtgatgactt tcttcgggct gccggcgacc gcgcgcgcct ccttcgcctg
      301 ctacaacacc gaggaggatg tggaacggct ggtgcgtgcg ctcgagcgcg cgggcgaggt
      361 gttcgcctga tggatctgaa ggagctctac cgggacgtga ttctcgatca caaccgccgt
      421 ccgcgtaact tcggcgcgct cgccggtgcc agccaccacg ccgagggcaa caatccgctg
      481 tgcggtgatc gcctgtcact cgcgctgcgc ctcgacggcg atcgcatcgc ggacattcgc
      541 ttcgaaggca gcggctgcgc aatctccacc gcctccgcct ccttgatgac cgaggccgtc
      601 aagggcaagg accgcgcggc cgtgcgccag ctcttcgagg aggtgcacac gctactcacc
      661 cagccggacg ctcccgccga tgccacgctc ggcaagctcg cggcgctctc gggggtacgc
      721 gagttcccgg cacgcgtgaa gtgcgcgagc ctttgctggc atacgctgaa cgcggcgctc
      781 tcctccagcg cggcgccggt ctccaccgaa tagcgccatg cgcagatacg agaacgaacc
      841 cttcgtcgtg agccgcgagg tgcgcgcggt gatcgtaccg gccggtcagg aggtgaagct
      901 caaacccggg caggccggct acatcacgca ggcgctcggc ggcagcttca ccgtctatct
      961 cgagggaaat ctgtttcgca tcgcgggcga ggacgcggat gccatcgggc aggagccggt
     1021 caagccgccg gagctgccgc ccaacgccag cgaggaggac gtgaaaggcc tcgcctggga
     1081 acagatgcgc acctgctacg atccggagat cccgatcaat atcgtcgacc tcggactggt
     1141 ctacgattgc gcggtcactc agaacgagga cggcacacgg acggtggacg tgacgctcac
     1201 gctgaccgcg cccggctgcg gcatggggga tatcctggtg gacgacgtac gcgacaagat
     1261 cagccgcatt cccacggtgc gtgaggcgcg cgtcagcctc accttcgatc cgccctggaa
     1321 ccagagcatg atgagcgagg cggcgcgcct gcagaccggc atgttctgag cgtgcgcgcg
     1381 cgagccgggg catgagttgg caagacgtgg ggcccgctgc cgccgtgcgc gagtcggcgc
     1441 cgctgtcggt ggaggtcgag ggcgtgcatc ttgccatcgt gcgctgcggc gatgcgttgt
     1501 acgctgtcga ggaccgatgc acgcacgatg gcgaatcgct gggggaggcg caggtggagg
     1561 actgccagat catctgtccg cgtcacggcg cgcatttctg cctgcgtacg ggcgaggcgc
     1621 tcaccccgcc ggcgtacgag ccgctgcgca cctatcgggt gcgcgagcag gacggacggc
     1681 tgctcatcga gaatccgtcg gagtgaggtg acggtgaagc ggctcacact catgcgtcac
     1741 gcggacgcac gctggcagga tgcgagaatg tcggatctcg agcgccccct caaccggcgc
     1801 ggcaccgcag ccgccgaggc gatggcgcgg cggctgctcg agctcgcgct cgtgccgcag
     1861 ctgctgctcg tgagtcccgc gcgccgcacg cagcagaccg gggagatcgt ggcgggagtg
     1921 ctggcgctgc cggcgcgact ggtcatctcg gacgaggggc tatacctcgc gagcgccgcg
     1981 gacctgctca aggtagtgca ggcgaccggg ccgcgcgtcg cgcatctgct cgtggtcggc
     2041 cacaatccgg gcgtgtccga gctggtgcag cagctcgtgc ccgaggcgga ggcgagtggg
     2101 ctcgcgacgg cggcgctgtg ctcgatcgcc ttcgagtgcg atcaatggcc cgcggtcggc
     2161 gtcgccgccg tcaaggatgt catgcgcgag gccccgccct cccggcggct cttctggctc
     2221 ttcggctgag tgaaacgctg cgcgcgcacc aagcaggctc agcactcggg tacgttgacg
     2281 gcgagccccc cctgcgaggt ctccttgtag atggtggaca tgtcggcgcc cgtctgccgc
     2341 atggtggcga tcacctgatc gagcgagacc ttgtgcgagc catcgccgcg catggcgagg
     2401 cgcgccgcgt ttaccgcttt caccgcaccc attgcgttgc gctcgatgca ggggatctgc
     2461 accaggccgg ccacgggatc gcaggtcatg cccagatgat gctccatccc gatctcggcc
     2521 gcattc
//
LOCUS       DATDMP010000199         2959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1141164, whole genome shotgun sequence.
ACCESSION   DATDMP010000199 DATDMP010000000
VERSION     DATDMP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141164"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VI195_03375"
     CDS             complement(<1..646)
                     /locus_tag="VI195_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823455.1"
                     /translation="MLTPAEADQLIGQHLQCLPIESLPLAQCAGAVLRENIYAERDQP
                     PYHRVAMDGVALDSQAVVAGSRNFRIQAMQAAGDAPLILDSPADCIEVMTGAVLPSGC
                     DSVVPIEQLTMQRGQAQLAIDARVEPWGNVHRRGTDTRQGALLLRAGQRLRAPEIAIA
                     ASAGMARIRVASQPMLAVISTGNELVEPGEPVLAHQVRRSNAYAIVSALREHGFQ"
     gene            complement(647..1021)
                     /locus_tag="VI195_03380"
     CDS             complement(647..1021)
                     /locus_tag="VI195_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823456.1"
                     /translation="MKALFAIALTAALTAAPAYADCPYPAAPAKLPDGATATMQDMID
                     GQNAVKGYDKAINDYVGCIDKELDDAIAKAGKDLKPEQKKHMQELEAQKHNAAIDELQ
                     NVATRFNEQVKIFKAKAADKKG"
     gene            complement(1082..1759)
                     /locus_tag="VI195_03385"
     CDS             complement(1082..1759)
                     /locus_tag="VI195_03385"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY6823457.1"
                     /translation="MAKPSRTTLHRAPRGRQPARRRCWWAETAAADYVRYHDEEWGRP
                     VHDERRLFEMLTLEGAQAGLSWSTILRKRAGYRRAFANFNVRRVADFDARRRAALRND
                     GAIVRNRLKIDSTVSNARAFLAVQREFGSFDRYLWSFAGGHPRVNRPRRGEVPARTAL
                     SDALSKDLVQRGFRFVGSTIVYAFLQAVGVVNDHTRDCYLCPQVRSRRARSRRPRPPA
                     RSRTHLQ"
     gene            1894..2673
                     /locus_tag="VI195_03390"
     CDS             1894..2673
                     /locus_tag="VI195_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823458.1"
                     /translation="MTDPSPRGTLLAATLCLLGAALAWPGRTRADAGPPFLTNDPGTP
                     GNGNWEINIAAQQTTQHGLSVWQLPQLDINYGVGERIQLTAEIPYVVLNSTGQPRASG
                     WGNANPGVKWRFFVQGESGWQISTFPTYQTGGSADAQRKGIAVEGPRIFLPLAVARKV
                     GRLSLNLEVGTYVPIHGEHERVIGLVAGQQITSGLELDVELYNDYVHGGEVDITTLDV
                     GGRYKLHRGVQPAIHGGPQLERQFPRAGRVHGLSRHPDSAQ"
     gene            2756..2829
                     /locus_tag="VI195_03395"
     tRNA            2756..2829
                     /locus_tag="VI195_03395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2790..2792,aa:Arg,seq:tcg)
BASE COUNT          511 a          952 c          980 g          516 t
ORIGIN      
        1 gctggaaacc gtgctcgcgc agcgcactca cgatggcgta tgcgttcgag cggcgcacct
       61 gatgagcgag caccggttca ccgggctcga ccagctcgtt gcccgtggag atgacggcga
      121 gcatcggttg gctcgcgacg cggatgcgcg ccatgccggc ggaggcggca atggcgattt
      181 ccggggcgcg cagacgctgg cctgcgcgca gcagcagtgc gccctgacgg gtatcggtgc
      241 cgcggcgatg cacgttcccc cagggctcga cccgtgcatc gatcgccaac tgtgcctgac
      301 cgcgttgcat cgtcagttgc tcgatcggca ccacgctgtc acagcccgat ggcaacaccg
      361 cgccggtcat cacctcgatg cagtcggcgg gcgagtcgag aatgagcggc gcatcgccgg
      421 ccgcttgcat cgcctgtatg cggaagttac gcgaacctgc aacgaccgcc tggctgtcga
      481 gcgccacgcc atccatcgcc acgcggtggt acggcggctg gtcgcgctcc gcgtagatgt
      541 tctcgcgcag caccgcaccg gcgcattgcg ccagcggcag tgactcgatc ggcaggcact
      601 gcagatgctg gccgatcagc tggtctgcct cggccggggt gagcactcag cccttcttgt
      661 cggccgcctt ggccttgaag atcttcacct gttcgttgaa gcgagtggcc acgttctgca
      721 gttcatcgat ggcggcattg tgcttttgcg cctcgagttc ctgcatgtgc ttcttctgct
      781 ccggcttgag gtccttgccg gctttggcga tggcgtcatc gagctccttg tcgatgcagc
      841 cgacataatc gttgatcgcc ttgtcgtagc ctttaacggc gttctggccg tcgatcatgt
      901 cttgcatagt cgcagtggcg ccatccggga gcttggcggg cgcggccggg tacgggcagt
      961 cggcataggc cggcgccgcc gtgagcgccg cggtgagcgc gatcgcgaaa agagccttca
     1021 tagccaaatc ctgatgtttt gaaatcggac cgctatctta agaggcgctc gggagcggac
     1081 gctactgcaa gtgtgtgcga gagcgtgccg gtggcctcgg ccgcctggac cgggcgcggc
     1141 gcgagcggac ttgcgggcac agatagcaat cgcgggtatg gtcgttcacg acccccaccg
     1201 cctgcaggaa ggcgtaaacg atggtcgagc cgacgaagcg aaacccgcgc tgcaccagat
     1261 ctttcgagag ggcatcgctc agcgcggtgc gtgccgggac ctccccacgg cggggacggt
     1321 tgacccgggg gtggccaccg gcgaaggacc acaggtagcg gtcgaaggaa ccaaactcac
     1381 gctgcacggc caggaaggcg cgggcgttgc tgacggtgga gtcgatcttc agccggttac
     1441 gcacgatagc gccgtcattg cgcagcgcgg cccggcggcg ggcgtcgaaa tccgccacgc
     1501 gccgtacgtt gaaattggca aaggcgcggc ggtaaccggc acgtttgcgc aggatggtcg
     1561 accaggagag ccccgcctgc gcaccttcga gcgtgagcat ctcgaacagt cgccgctcat
     1621 cgtgcaccgg gcgcccccat tcctcatcgt ggtagcgcac atagtcggca gcggcggtct
     1681 ccgcccacca acagcggcgc cgtgcaggct ggcggccgcg cggcgcgcgg tgcagggtcg
     1741 tcctgctcgg cttggccatg ctcggctcct tcatgtggct gctgcagcgg ggcggtgtct
     1801 gccatcatga cagactacgc cgccggcaga tccccccggt ggggcaactc tgacactgca
     1861 ccacagcggc agcgtacaat ccccgagcca acaatgaccg atccctcgcc gagaggcacg
     1921 ctgctcgctg ccaccctgtg cctgctcggc gccgcgctcg cctggcccgg ccgcacgcgc
     1981 gccgacgccg gtccgccatt tctcaccaac gacccgggca cgccgggcaa cggcaactgg
     2041 gagatcaaca tcgctgccca gcagacgacg cagcacggcc tgtcggtctg gcagcttccg
     2101 caactcgaca tcaactacgg cgtcggtgag cgcattcagc tcaccgcaga gattccgtac
     2161 gtcgtactga acagtaccgg gcagcctcgg gcgagcgggt ggggtaacgc caacccgggc
     2221 gtgaaatggc ggttcttcgt tcagggcgag agcggatggc agatctccac ctttcccacg
     2281 taccagactg gtggctcggc ggatgcgcag cgcaaaggca tcgccgtaga ggggccgcgc
     2341 attttcctgc cgctcgcggt ggcccgaaag gtcggcaggt taagtctcaa cctggaggtc
     2401 ggcacctacg tgcccatcca tggggagcat gagcgcgtca tcgggctcgt cgccggacag
     2461 cagatcactt cgggccttga gctcgatgtc gagctctaca acgattacgt gcacggcggg
     2521 gaggtggaca tcacgactct cgatgtgggc ggccgctaca agctgcaccg cggcgttcaa
     2581 cctgctattc atggcgggcc gcagcttgag cggcaattcc cccgggcagg tcgagttcat
     2641 gggctatctc ggcatccaga ttctgctcag tgattacggg cgcagattga gtgaggaccc
     2701 atgaggcacg tcgtcaccga agctgtacgc tcgcccgtag tagaatcggc gcctcgcgcc
     2761 cgtagctcag tcggatagag catcaggctt cgaacctgag ggtcgggagt tcgaatctct
     2821 ccgggcgcgc agtgttctaa gcgattcgtt gaaaacctga gcaaccactg agcaaaccag
     2881 caggcgtcat catgactttc gagaccaccg gcgacggtca aaacacgaca actgggaagt
     2941 ggcaagtgcg tattcaggg
//
LOCUS       DATDMP010000200         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2029977, whole genome shotgun sequence.
ACCESSION   DATDMP010000200 DATDMP010000000
VERSION     DATDMP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029977"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VI195_03400"
     CDS             complement(<1..702)
                     /locus_tag="VI195_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY6823459.1"
                     /translation="MKIADARERTVPIGAPMRNAAIAFDTMTASAVALTTDAGLTGYA
                     FDSIGRYGKGALLRERFFPRLRSAPSDALLDEEGFTDPPALARVAMSNEKPGGHGERS
                     GAIGLIEAAAWDLRAKQQQLPLWASLADYYRTKGAGPSISVYASCGHFRTDDILTDEV
                     RRAIDAGYRCVKIKVAGDTASDGKRLERAAAALDPKAQWAVDLNGALVRERAAQWFDT
                     VQPFKLAWIEEPAPPL"
     gene            complement(689..2935)
                     /locus_tag="VI195_03405"
     CDS             complement(689..2935)
                     /locus_tag="VI195_03405"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6823460.1"
                     /translation="MEQRVAPSTVGRAIPRSHARRLVAGRGAYADDLRFPRLLHAAFL
                     RSPHARARIARLDVSAARTAEGVFAAYAAAALAAVCKPWQTKLANWPGHQSPAQPPLA
                     SGEARWQGQPVALVLATSRAAAEDAVERIEVEWEPLAPIPHREAALEGETNLAFEHTV
                     AAGSAPAGTATVRRTVTFARHTGVPLEPRTIVASFDPGERKLTVHQATQVPHQMRAVY
                     ADHLGLAEQDVRVITPDIGGGFGVKLHVYDDEMAVAAASMLAGRAVKYVCDRLEAFVS
                     DIHARAHTVSVSAALDTSGRIHDLQLDAVVEAGAYSAYPRSSILEGLQVLLMAGTPYQ
                     IDAYEGRLRVVWQNKPATGSYRGVGQPVACVAMELLMDACARERGIDPAEFRRRNFRR
                     EGATPTHVQAGGLSHEMCLDRLLELMDYRTLRDEQQRARSAGRQLGIGFATFVEQNAP
                     GPAFYGAAGVKISAQEGCTLRLEPSGTLTCITSNQDQGQGVETALAQLVAETLRVPFE
                     NVRVIGGNTAMTAVGGGTFASRGLTIAGEAVLTAARAMAERIARLREVLNLPDAPIEE
                     IAAIMNYQQHILPPGLDASPAVTTHVFMRVPFLLANGIQASLVEVDVATGFVKLLKHW
                     VVEDCGQVINPLLADEQIRGGVVQGIGAALYEECLYDESSQLLNGSLADYLVPMALEM
                     PDIVVAHVETPVAGTALGAKGIGEAGTIGAAAAVGNAINDALAPFGAEVLQMPYTPQR
                     ILAALENR"
BASE COUNT          465 a         1027 c         1060 g          465 t
ORIGIN      
        1 gagcggcggc gccggttcct cgatccacgc gagcttgaat ggctgcacag tatcgaacca
       61 ttgcgcggcc cgctcgcgca caagcgcgcc gttcaggtcc acggcccatt gcgccttggg
      121 atcgagcgcg gcggcggcgc gctccaggcg ctttccgtcg gacgccgtgt cgcccgccac
      181 cttgatcttc acgcagcggt agccggcgtc gatcgcgcgg cggacttcgt cggtcaggat
      241 gtcgtcggtg cgaaagtggc cgcacgacgc atagaccgag atcgatggcc ccgcaccttt
      301 cgtgcggtag tagtcggcga gcgaggccca gagcggcagc tgttgctgct tcgcgcgcag
      361 gtcccacgcc gctgcttcga tcaagccgat ggcgccggag cgttcgccat ggccgcccgg
      421 cttttcgttc gacatggcga cgcgcgccaa ggccggcggg tcggtgaatc cttcctcatc
      481 gaggagcgcg tcggacggcg cgctgcgcag gcgcgggaag aagcgctcgc gcagcagcgc
      541 gcctttgccg tagcggccga tcgagtcgaa agcatagcca gtgagcccgg cgtccgtggt
      601 gagcgcaacg gccgaagcgg tcatcgtgtc gaaggcgatg gcggcgtttc gcatcggcgc
      661 gccgatcggc acggtgcgct cgcgggcgtc agcgattttc aagggcggcc agtatgcgtt
      721 gaggcgtgta gggcatctgc aacacctcgg cgccgaaagg cgcgagcgcg tcgttgatcg
      781 cgttgccgac cgccgcggcg gcgccgatgg tgccggcctc gccgatgccc ttggcgccga
      841 gcgcggtgcc ggcgaccggc gtctcgacgt gcgcgacgac gatgtccggc atctcgagcg
      901 ccatcggcac caggtagtcg gcaagggagc cgttcagaag ctggctcgat tcgtcgtata
      961 agcactcttc atagagcgcc gcgccgatgc cctgcaccac gccgccccgg atctgctcat
     1021 ccgccaagag aggattgatc acctggccgc aatcctcgac cacccagtgc ttgagcagct
     1081 tcacgaagcc ggtagcaacg tccacttcca ccaggcttgc ctgaatgccg ttggcgagca
     1141 ggaacgggac gcgcatgaag acatgcgtcg taacggcggg actggcatcc aggcccggcg
     1201 gaaggatgtg ctgctggtaa ttcatgatgg ccgcgatctc ctcgatcggc gcatcgggca
     1261 ggttcagcac ctcgcgcagc cgcgcgatgc gctcggccat ggcccgcgcg gcggtcagca
     1321 cggcttcacc ggcgatggtg aggccgcgcg acgcgaacgt gccgccaccg acggcggtca
     1381 tcgcggtgtt gccgcctatg acgcgcacgt tctcgaaggg aacccgcaag gtctcggcca
     1441 cgagctgcgc gagcgccgtc tccacgccct gcccttggtc ctgattcgac gtgatgcagg
     1501 tgagcgtgcc cgagggctcg aggcgcaacg tgcaaccttc ctgcgccgag atcttcactc
     1561 ccgccgcacc gtagaaggcg gggcccggcg cgttctgctc gacgaacgtc gcgaaaccga
     1621 tgccgagctg gcggccggcg ctgcgcgctc gttgctgctc gtcgcgtagc gtccggtagt
     1681 ccatgagttc gagcaggcga tcgagacaca tctcgtgcga caggccgccc gcctgcacgt
     1741 gagtcggcgt ggcgccttcg cgccggaagt tgcgccggcg aaattcggcc gggtcaatgc
     1801 cgcgctcgcg cgcgcaggcg tccatcagca attccatcgc cacacatgcc accggctgac
     1861 cgacgccgcg ataagaaccg gtcgccggct tgttctgcca aacgacgcgc aggcgcccct
     1921 cgtaggcatc aatctgatac ggcgtgcccg ccatcagcag cacctgcagt ccttcgagga
     1981 tgctcgagcg cggataggcg gaataggcgc cggcctcgac caccgcatcg agctgcaggt
     2041 catggatccg gccgctcgtg tcgagggcgg cagacacgct caccgtgtga gcgcgcgcat
     2101 gaatgtcgga gacgaatgcc tccaggcgat cgcacacgta cttgacggcg cgcccggcga
     2161 gcatggatgc cgcggccacc gccatctcgt cgtcatagac gtggagcttg acgccgaagc
     2221 cgccgccgat gtccggcgta atgacacgca cgtcctgctc cgccaggccg agatggtcgg
     2281 cgtagacggc gcgcatctgg tgcggcacct gcgttgcctg gtggaccgtg agcttgcgct
     2341 cgcccggatc gaacgaggcc acgatcgtgc gcggctcgag gggcaccccg gtgtggcgcg
     2401 caaaggtgac ggtacgccgc acggtggccg tgccggcggg cgcgctgccg gcggctacgg
     2461 tgtgttcgaa cgcaaggttg gtctcgccct cgagcgcggc ctcgcggtgc gggatcggcg
     2521 cgagcggctc ccactctact tcgatgcgct cgaccgcatc ctcggccgcg gcacggctcg
     2581 tcgccagcac gagcgcgacc ggctgccctt gccagcgtgc ctcgccggaa gcaagcggtg
     2641 gctgcgcagg cgactggtgg ccgggccaat tggcgagctt ggtctgccat ggtttgcata
     2701 ccgccgcgag cgctgcggcg gcgtacgcag cgaacacgcc ctcagcggta cgggctgccg
     2761 aaacgtccag gcgagcgatg cgcgcccgcg catgcggact acgcaggaac gccgcgtgca
     2821 gcagccgcgg gaaccgcagg tcgtcagcgt aggcgccgcg gccggcgacc agtcggcgcg
     2881 catgcgagcg cgggatggcg cgcccgactg tgctgggagc gacgcgctgc tccatcagag
     2941 cggtacggct atcgcgcggt gcggcgcctc gccatcgatg aggtcgacac gcttcaggac
     3001 gatgcgcagg ccgcctg
//
LOCUS       DATDMP010000201         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_364307, whole genome shotgun sequence.
ACCESSION   DATDMP010000201 DATDMP010000000
VERSION     DATDMP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364307"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VI195_03410"
     CDS             <1..1359
                     /locus_tag="VI195_03410"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY6823461.1"
                     /translation="LFPAEYVERAIASAPKSIALHDRDGRPYTELGGDKVHFVPGSSG
                     LQVLDHRTGETRHSNTRDFIEYVRVGDGLKNLGYLATAFSTDDIEPQVSDAWRLYLVL
                     TNSLKPVVSGAFTEHGVPRMAQMMQLFRRDQADLIARPMSIFTVTATGFYRYSEDSCQ
                     NLLDCAEFGIPVEIVPVTLMGLIAPVTLVGATVMHVADVLAGITMAQMVRPGTPVLFG
                     GAPATFHMKTAMSPMAAIETLHLNAAYVEIAKHLGLPTQSYMALSEGKFLDAQAGAET
                     FAGALLAAITGVNSVSGPGMLDYVLTFSLEKLVFDDELCGQALHFCREFAVMDDLPTV
                     ELVRQLLQEKHLLTATHTLSYWPRELYLPGAVFDRVNRETWLQEGSRSLDQRVKAEVE
                     RLLAVYASPPTDPLIDAEMIRMIKAGLTAGQALPETLPPAHPSATAGPVRERRGRGRR
                     PA"
     gene            1495..1947
                     /locus_tag="VI195_03415"
     CDS             1495..1947
                     /locus_tag="VI195_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823462.1"
                     /translation="MKKTVDAFVGHWKLTGTDLEPGATAPAVVNGTMDCKSAASGAAV
                     SCLIAAEISGTRIEAATVIGYSPDEHMVRWMEISSTGEYHDHHGRWQGERIVFEPLTY
                     TAGGTKMTEHFAVSFASADRMTLKATTKMLQGESRLELTGVRVNAKAR"
     gene            complement(1985..>2270)
                     /locus_tag="VI195_03420"
     CDS             complement(1985..>2270)
                     /locus_tag="VI195_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6823463.1"
                     /translation="IEELRRHDLYDRVSQAFAVFLPVRSVGVMGDGRRYDYVIALRAV
                     ETVDFMTAHWARLPYEFLDHVSRRIVNEVAGISRVVYDISGKPPATIEWE"
BASE COUNT          401 a          742 c          739 g          388 t
ORIGIN      
        1 ctgtttcccg cggagtacgt ggaacgggcg atcgcgagcg cccccaagtc gattgcgctc
       61 cacgaccgcg acggccggcc gtacaccgag ctcggcggcg ataaggtgca tttcgtcccc
      121 ggctcgagcg ggctgcaggt gctcgaccat cgtaccggcg agacgcgtca ctccaacacg
      181 cgtgatttca tcgagtatgt gcgggtcggc gatgggctga agaacctcgg ttacctggcg
      241 accgcgtttt ccaccgacga catcgagccg caggtctcgg acgcctggcg gctgtacctg
      301 gtgctcacca actcgctgaa gccggtggtg tcgggagcgt tcaccgagca cggcgtcccg
      361 cgcatggcgc agatgatgca gcttttccgg cgcgaccagg ccgatctcat cgcgcgaccc
      421 atgtcgatct tcaccgtcac cgccaccggc ttctaccgct acagcgaaga ctcctgccag
      481 aacctgttgg attgcgccga gttcggcatc ccggtggaga tcgtgccagt gaccctgatg
      541 ggcctgatcg caccggtgac cctggtcggg gccaccgtga tgcacgtcgc cgacgtgctc
      601 gccggaatca ccatggcgca gatggtgcgg ccgggcacgc ccgtgctctt cggcggtgcg
      661 ccggcaactt tccacatgaa gaccgccatg tcgcccatgg cggcgatcga gacactgcac
      721 ttgaacgcgg cctacgtgga gatcgccaag cacctgggtc tgcccacgca gagctacatg
      781 gcgctgagtg aaggcaagtt tctcgatgcg caggccggag cggaaacctt cgccggtgcc
      841 ctgctggcgg ccatcaccgg agtcaactcg gtgtcgggac ccggcatgct cgactacgtg
      901 ctcaccttca gcctggagaa gctcgtgttc gatgacgagc tctgcggtca ggcgctgcac
      961 ttctgtcggg agttcgcggt gatggacgac ctgccgacgg tggagctcgt gcgccagctg
     1021 ctgcaggaga agcacctgct caccgcgacg cacacgctca gttactggcc gcgggagctg
     1081 tacctgccgg gggcagtgtt cgaccgggtc aatcgcgaga catggctcca ggaaggcagc
     1141 aggagcctcg atcaacgggt caaggccgag gtcgagcgac tgctcgccgt ctacgcttcg
     1201 ccgcccaccg atccgctcat cgatgcggag atgatccgga tgatcaaggc gggcctcacc
     1261 gccgggcagg ctctgccgga gacgcttccg ccggcgcacc cgagcgccac agcaggaccg
     1321 gtacgcgagc ggcggggtcg gggccggcgt ccggcgtaag ccgcgaggct ctccgcgcgg
     1381 cgccgaacgt caatgcaaag gaaactctta ggctggtgtc gggtcccggg gctcttgctc
     1441 ggcgcgtcgt cgctcctcgc ccatgcagac agcgctccgc cctcaccgcc cgaagtgaag
     1501 aagacggtcg atgcgttcgt tgggcactgg aaattgaccg gcaccgacct ggaaccgggg
     1561 gcaaccgcgc ctgcggtcgt gaacggcacc atggactgca agtccgcggc atcgggcgcg
     1621 gcggtgagct gtctcattgc tgccgagatc tccggcacac gcatcgaggc tgcgactgtg
     1681 atcggctaca gccccgatga gcacatggtg cgatggatgg aaatttcctc tacgggcgaa
     1741 tatcacgatc atcacggacg gtggcagggc gagcggatcg tgttcgagcc cctcacttac
     1801 acggcgggcg gcacgaaaat gaccgagcac tttgcggttt cctttgcctc agccgacagg
     1861 atgacgttga aggcgaccac caagatgctc cagggcgaat cccgtctgga gctcaccggt
     1921 gtgcgcgtga acgccaaagc tcgttgacgc gacggatgtc gctttacttt catcctccgg
     1981 atcctcactc ccactctatg gtcgcgggcg gcttgccgga gatgtcgtag acgacgcgcg
     2041 agataccggc gacttcgttg acgatacgcc gcgagacgtg atcgaggaac tcgtacggca
     2101 ggcgcgccca gtgagcggtc atgaaatcca ccgtttccac cgcccgcagc gcgatcacgt
     2161 agtcgtagcg gcgtccatcg cccatgaccc cgaccgagcg cactggcagg aataccgcaa
     2221 acgcctggct cacgcggtcg tacagatcgt gacggcgcag ctcctcgatg
//
LOCUS       DATDMP010000202         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1253488, whole genome shotgun sequence.
ACCESSION   DATDMP010000202 DATDMP010000000
VERSION     DATDMP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253488"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VI195_03425"
     CDS             complement(<1..265)
                     /locus_tag="VI195_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY6823464.1"
                     /translation="MRYSIFSVNDHYPRRARTVPQLYQQVMHSCELAERLGFDTFFCA
                     EHHFHEYGVVPDPAVMLSALAQRTKRIRLGTAISILTFHDPRRI"
     gene            complement(289..1875)
                     /gene="aceB"
                     /locus_tag="VI195_03430"
     CDS             complement(289..1875)
                     /gene="aceB"
                     /locus_tag="VI195_03430"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY6823465.1"
                     /translation="MTISAPTAQDIRVAGRGSAEFSQILSEPALDFIAKLHRQFEPRR
                     QELLARRAARQKEFDAGKRPDFLAETKSIRESEWRVASQPKDMLDRRVEITGPTDRKM
                     VINALNCGASTFMADFEDANCPTWHNMVDGQLNLRDAVRRTISFEQGGKNYQLKDKTA
                     VLIPRPRGWHLDEKHVTVDGKPISGAIFDFGLFFFHNAKELLARGSGPYFYLPKMESH
                     LEARLWNEIFVAAQAALGIPRGTIRATALIETVVAAFEMDEILYELREHSAGLNIGRW
                     DYIFSCIKKFRADQAFCLADRSQVTMLAPFMRAYALLLVKTCHRRGAPAMGGMAAQIP
                     IKNDPAANEAALEKVRQDKLREVTDGCDGTWVAHPGLVPIAKEIFDKHMPQPNQYGRQ
                     RPDVNVSAKDLLNFQPERPITEAGLRNNISVGIQYLGAWLAGNGCVPVFNLMEDAATA
                     EISRSQIWQWIRSPKGVLEDGRKVTVELFRQLLAEELAKVKSAQAEGRYDEAAKLFDR
                     LTSDDRYVEFLTLPAYQQLD"
BASE COUNT          354 a          671 c          714 g          394 t
ORIGIN      
        1 caatacgacg cgggtcgtgg aacgtcagga tggagatcgc ggtgcccagg cgaatgcgct
       61 tggtccgctg cgccagcgcc gacagcataa ctgccgggtc gggtaccacg ccgtattcat
      121 ggaaatggtg ctcggcgcag aaaaacgtgt cgaagcccag gcgttccgca agctcgcagg
      181 aatgcatcac ctgctggtag agctgcggca ccgtgcgagc acggcgcggg tagtgatcgt
      241 tgaccgagaa aatggagtag cgcatcgcgc accccatttt accggcggtt agtcgagctg
      301 ctggtacgcg gggagagtga ggaactcgac gtagcggtcg tcgctggtga ggcgatcgaa
      361 gagcttcgcc gcctcgtcgt agcggccctc ggcctgcgcg ctcttcacct tcgcgagctc
      421 ttcggccagc agctggcgga acagctcgac cgtcaccttg cggccgtctt cgagcacgcc
      481 cttgggtgag cggatccact gccagatctg cgagcgcgag atttccgcgg tggcggcatc
      541 ttccatcagg ttgaagaccg gcacgcagcc gttgccggcg agccaggcgc cgaggtactg
      601 aatgccaacc gagatgttat tgcgcagtcc cgcctcggtg atcggccgct cgggctggaa
      661 gttgagcagg tccttcgcgc tgacgttgac gtcgggccgc tgccggccgt actggttcgg
      721 ctgcggcatg tgcttgtcga agatctcctt cgcgatcggc acaaggcccg gatgggcgac
      781 ccaggtaccg tcgcagccgt cggtcacttc gcgaagcttg tcctggcgca ctttctcgag
      841 cgccgcctcg ttcgccgccg gatcgttctt gatcgggatc tgggccgcca tgccgcccat
      901 cgccggcgcg ccgcgccggt ggcaggtctt cacgagcagc agcgcatagg cgcgcatgaa
      961 cggcgcgagc atcgtcacct ggctgcggtc ggcgaggcag aacgcctggt cggcgcggaa
     1021 tttcttgatg cacgagaaga tgtagtccca gcgaccgatg ttcaagccgg cggagtgctc
     1081 gcgcagctcg tagaggatct cgtccatttc gaacgccgcg accaccgtct cgatgagcgc
     1141 ggtggcgcgg atggtgccgc gcgggatgcc gagcgccgct tgcgcggcga cgaagatctc
     1201 gttccacaag cgcgcctcga ggtgcgactc catcttcggc agatagaaat acgggcctga
     1261 cccgcgcgcc agcagctcct tggcgttgtg gaagaagaac aggccgaagt cgaagatcgc
     1321 gcccgagatc ggcttgccgt ccacggtgac gtgcttctcg tcgaggtgcc agccgcgcgg
     1381 gcgcgggatg agcaccgcgg tcttgtcctt caactggtaa ttcttgccgc cctgctcgaa
     1441 gctgatggtg cggcgaaccg cgtcgcgcag gttgagctgc ccgtcgacca tgttgtgcca
     1501 ggtcgggcag ttggcgtcct cgaaatccgc catgaaggtc gaggcgccgc agttcagcgc
     1561 gttgatcacc atcttgcggt cggtcggccc ggtgatctcg acgcggcgat cgagcatgtc
     1621 tttgggctgc gacgcgacgc gccactccga ctcgcggatg gacttcgtct ccgccaggaa
     1681 atcgggccgc ttgccggcgt cgaattcctt ttgccgcgca gcgcggcgcg cgagcaattc
     1741 ctgccggcgc ggctcgaact ggcggtgcag cttggcgatg aagtcgagcg ccggctcgct
     1801 caggatctgc gagaactcgg cgctgccgcg gccggcaacg cgaatatcct gcgccgtagg
     1861 ggcggagatg gtcatcgcag cattttatga agcgatatgc ggcgccgcaa catgaaatgc
     1921 tagttctttc ggagtaccct cgcgttccca tcccgcagcg tcccattccg catcgtgaaa
     1981 agccatggcc tataacgtcc tgatcttcgg tgcttcctac gggtcgctac tcgcgaccaa
     2041 gctgctcgcc gccggtcaca acgcaaagct catctgcgtg ccgtcggagg cggagctgat
     2101 caacaaagag ggcacccgcg tgcggctgcc ggt
//
LOCUS       DATDMP010000203         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1364615, whole genome shotgun sequence.
ACCESSION   DATDMP010000203 DATDMP010000000
VERSION     DATDMP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364615"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /gene="glyS"
                     /locus_tag="VI195_03435"
     CDS             complement(<1..1691)
                     /gene="glyS"
                     /locus_tag="VI195_03435"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY6823466.1"
                     /translation="MSAARDFLIEVGTEELPPKALSTLSQAFAAGISAGLEKSGLAHG
                     DIAAFATPRRLAVRVSRLAAQQPEQQIRRRGPPLSAAFDGAGAPTRAALAFAASCGST
                     VEALGRIEEEKGTFLFFGGTRAGERTVQLLPALIQSALDQLPIPRRMHWGEGQAMFVR
                     PVHWIVMLFGRDVVPATLLETASGRHTRGHRFLAPRPLTLASPASYERTLREKGHVLA
                     DFSARRERIRTEVTTVAEAVAGRALIREALLDEVTALTEWPVALAGRFEERFLALPRE
                     VLICTLEDHQRYFPVEDSAGRLLPAFITVSNIASRDPPQVRAGNERVVRARLSDAAFF
                     WEQDRREPLAARRLALESVTFQAKLGSLGDKTRRVRGLIPQIAAASGAPAEEAVRAAD
                     LAKCDLLTAMVAEFPELQGIMGAYYAQADGEPPQVALSLREQYLPRSAGDALPSSGTG
                     LALAIADKLDTLAGIFAIGEKPSGTKDPFALRRAALGVQRILIEKGLDLDLRATLERA
                     VAGVRADIERLRSVGPAGTTGSFGAPPAATPSSAKAQPGTAALVDEIYDFLMERLR"
     gene            complement(1688..2677)
                     /gene="glyQ"
                     /locus_tag="VI195_03440"
     CDS             complement(1688..2677)
                     /gene="glyQ"
                     /locus_tag="VI195_03440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY6823467.1"
                     /translation="MKIGAAPRSFQQLIFSLQHYWSQQGCVVLEPYDMEVGAGTFHTA
                     TFLRAIGPEPWRAAYVQPSRRPTDGRYGENPFRLQHYYQYQVVIKPSPLPILDLYLGS
                     LAALGLDPLVHDVRFVEDNWESPTLGAWGLGWEVWLNGMEITQFTYFQQVGGLECRPV
                     TGEITYGLERLAMYLQGVESIYDILWSEGIDGPITYGDVFHQNEVEQSAYNFEQADVS
                     TLLTHFENHERACQSLLQAKLALPAYEQVLKASHTFNLLDARRAISVTERQRYILRVR
                     TLASAVARAYLASREALGFPLLKAHEAHAARIAHSMAARGAATADASTPRARP"
BASE COUNT          472 a          988 c         1005 g          436 t
ORIGIN      
        1 cgcagccgct ccatgaggaa atcgtagatc tcatccacca gcgccgcggt ccccggctgc
       61 gccttggccg aggagggcgt tgccgccggc ggggcgccga aactccctgt cgtgccggcc
      121 ggtcccacac tcctcagccg ctcgatgtcg gcgcgcacgc cagccaccgc ccgctcgagg
      181 gtcgcgcgca gatcgagatc gagtcctttc tcgatgagga ttcgctgcac gccgagcgct
      241 gcgcggcgca atgcgaaggg atccttggtg ccggagggct tttcgccgat tgcgaagatg
      301 cccgcaagcg tgtcgagctt gtccgcgatc gcgagtgcga gccctgtacc gctcgagggg
      361 agcgcatcgc ccgccgagcg gggcaggtac tgctcgcgca ggctgagggc cacctgcggc
      421 ggctcgccgt ccgcctgcgc gtagtaggcg cccatgatgc cctgcagctc cggaaactct
      481 gccaccatgg cggtgagcag atcgcacttg gcgagatccg cagcccgcac cgcctcctcg
      541 gccggcgcac ccgacgccgc ggcgatctgc gggatgagcc cacgcacacg gcgggtcttg
      601 tcgccgagcg agccgagctt cgcctggaag gtcacgctct cgagcgccag gcggcgcgcg
      661 gcgagcggct cgcgccggtc ctgctcccag aagaacgcgg catcggacaa gcgcgcgcgc
      721 acgacgcgct cattgccggc tcgcacttgc ggcggatcgc ggctggcgat gttgctcacc
      781 gtgatgaaag ccggcagcag gcggcccgcg ctgtcctcga cgggaaaata acgctggtga
      841 tcctccagcg tgcagatcag gacctcgcgc ggcagcgcga ggaagcgctc ctcgaagcgc
      901 cccgcgagtg ccaccggcca ctcggtgagt gcggtcacct catcgagcag cgcttcacgg
      961 atgagcgcgc ggcctgcgac cgcctcggcg acggtggtga cttcggtgcg gatgcgctcg
     1021 cggcgcgcgc tgaaatccgc gagcacgtga cccttctcgc gcaaggtacg ctcgtagctc
     1081 gcggggctcg cgagcgtgag cgggcgcggc gcaagaaagc gatggccgcg ggtgtggcgg
     1141 ccgcttgcgg tttcaaggag cgtcgcgggc acgacatcgc gcccgaagag catcacgatc
     1201 caatgcaccg ggcgcacgaa catcgcctga ccctcgcccc agtgcatgcg gcgcggaatc
     1261 ggcagctggt cgagcgcgct ctggatgagc gccggaagca gctgcacggt gcgctcgcct
     1321 gcgcgcgtgc cgccgaagaa gaggaacgtg cctttctctt cctcgatgcg cccgagcgcc
     1381 tcgacggtag aaccacagct cgccgcaaag gcgagcgccg cccgggtcgg tgcccccgca
     1441 ccatcgaatg cagcggagag cggcggaccg cgacggcgga tctgctgctc gggctgctgc
     1501 gcggcgagcc gcgacactct cacggcaagg cgcctcgggg tggcgaaagc tgcgatgtcg
     1561 ccgtgggcga gaccggattt ctcgagtccg gcgctgatcc cggcggcgaa ggcctgcgac
     1621 agtgtgctca atgccttcgg cggcagctcc tcggtgccga cctcgatgag gaaatcgcgc
     1681 gccgcactca tgggcgcgcc cgcggagtcg aggcgtccgc ggttgcagcc ccacgggcgg
     1741 ccatcgagtg cgcgatgcgc gctgcatgcg cctcgtgcgc cttgaggagc ggaaagccga
     1801 gcgcctcgcg gctcgcgaga taggcgcgcg ccacggcgct cgcgagcgtg cgcaccctga
     1861 ggatgtaccg ctgccgctcg gtgaccgaga tcgcgcgccg ggcatcgagc aggttgaaag
     1921 tgtgcgaagc cttcagcacc tgctcgtagg ccggcagggc gagcttcgcc tgcagcaggc
     1981 tctggcaggc gcgctcgtga ttctcgaagt gggtgagcag cgtcgacaca tccgcctgct
     2041 cgaagttgta ggccgactgc tcgacctcgt tctggtggaa cacgtcccca tacgtgatcg
     2101 gaccgtcgat cccctcgctc cagaggatgt cgtagatgct ctcgacaccc tgcaggtaca
     2161 tcgcgagccg ctcgaggccg taggtgatct cgccggtcac cgggcggcac tcgaggccgc
     2221 ccacctgctg gaagtaggtg aactgcgtga tctccatgcc gttcagccag acttcccagc
     2281 cgagtcccca cgccccgagc gtcggcgact cccagttgtc ctcgacgaaa cgcacatcgt
     2341 gcaccagcgg gtcgaggccg agtgccgcca gacttccgag gtagagatcg aggatcggca
     2401 gcggcgaggg tttgatcact acctggtact ggtagtagtg ctgcagccga aaaggattct
     2461 ctccgtaacg gccgtcggtg ggccgccgcg agggctgcac gtaggcggcg cgccacggct
     2521 caggtccgat cgcgcgcagg aaggtcgccg tgtgaaaggt gccggcgcca acttccatgt
     2581 cgtaaggctc gagcaccaca cacccctgct gcgaccagta gtgctgcagg ctgaagatga
     2641 gctgctggaa ggagcgcggg gctgcgccga ttttcatgct gtgtgagtga ggccgaaaga
     2701 gcgcgcgagt atacggcgct cgccacgggc gcggtgtcgg cgcaccacaa gcgcgcatct
     2761 gcactgccat ggacccgccg atcacccgct cgacagatcg cgggcccccc cgacggctga
     2821 cgagcgcacc ggcatggtgc aataatgcgc ccaatgtgca ccaagatggt gcacaacgtc
     2881 atctcgatag gtccctaact c
//
LOCUS       DATDMP010000204         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_809337, whole genome shotgun sequence.
ACCESSION   DATDMP010000204 DATDMP010000000
VERSION     DATDMP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809337"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VI195_03445"
     CDS             <1..396
                     /locus_tag="VI195_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY6823468.1"
                     /translation="AMIIAEFFFPTYGSLGVGGVIAFVVGSLILFDTDVPGLSVGRPL
                     IAAFATVGALMVGGIVYLGTRAMRHPVATGTEGMIGASAEVVADFSGKGKVRYGGELW
                     NARSERALRAGEPARIVKVEGLTLWVEPL"
     gene            422..1168
                     /locus_tag="VI195_03450"
     CDS             422..1168
                     /locus_tag="VI195_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY6823469.1"
                     /translation="MPFVIAVLILIVVLLFSAINVLNEYERAVVFTLGRFRGIKGPGL
                     VLIYPVVQRMRKVDLRVIVLSVPKQDVITRDNVSVKVNAVVYFRIVDPGKAIIQVANA
                     FDATSQVAQTTLRSVVGQHEMDDLLSQRDKLNVDLQRILDQSTEAWGIKVSTVEIKDV
                     DLDETMIRAMAKQAEAERTRRAKIINAEGVKQAAQTLVEAAQALAAAPSALQLRYLQT
                     LADIAHERTQLIVFPLPLDLIKPLLGPRGD"
     gene            1205..1639
                     /locus_tag="VI195_03455"
     CDS             1205..1639
                     /locus_tag="VI195_03455"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY6823470.1"
                     /translation="MPLDRQEAKSRGDPAGTRALALAGLLLALATVCGAFGAHALKSQ
                     LAPERLQLWDTAVRYQFFQALGLLGIGLTLRLLEPAGGVAAGALRAAAVLSAAGVLLF
                     SGSLYALALGASRGLGVLTPLGGIAWIAAWLLFAWGAWRAQW"
     gene            1662..>2075
                     /locus_tag="VI195_03460"
     CDS             1662..>2075
                     /locus_tag="VI195_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6823471.1"
                     /translation="MSTRTLSAALIAAGAMALAFVATPYISAAAAAAAGHAQLGSFGL
                     DLSGGDPSVKPGDDFARYANGRWLDTAQIPPDRASWGSFAMLRERSLQQVRDILEALP
                     NESPQGSNEQKLHDFYRSYLDTDAIERAGLSSAKAV"
BASE COUNT          311 a          699 c          702 g          363 t
ORIGIN      
        1 gccatgatca ttgcggagtt cttcttcccc acctatggct cgcttggagt gggcggcgtg
       61 attgcgttcg tcgtcggctc gctcatcctc ttcgataccg acgtgcccgg cctgagcgtc
      121 ggccggccgc tgatcgccgc gttcgccacc gtcggcgcgc tcatggttgg cgggatcgtc
      181 tacctgggca cccgcgccat gcggcatccg gtcgccaccg ggaccgaggg gatgatcggg
      241 gcgagcgccg aggtcgtggc cgacttcagc ggcaagggca aggtgcgcta tggcggtgag
      301 ctgtggaacg cgcgcagcga gcgtgcgctg cgcgccggcg agccggcgcg catcgtgaag
      361 gtggaaggtc taacattgtg ggtcgagccg ttgtaggcac gccaccaccc cggagcaccg
      421 catgccgttc gtgatcgccg ttctgatact catcgtcgtg ctgctgttca gcgccatcaa
      481 cgtgctgaac gagtacgagc gcgccgtcgt gttcacgctc ggccggtttc gcggcatcaa
      541 gggcccgggc ctggtgctca tctatccggt cgtgcagcgc atgcgcaagg tggatctgcg
      601 cgtaatcgtg ctgagtgtgc cgaagcagga cgtgatcacg cgcgacaacg tatcggtcaa
      661 ggtgaacgcg gtggtctact tccgcatcgt cgatcccggc aaggccatca tccaggtggc
      721 gaatgcgttc gacgccacca gccaggtggc gcagaccacg ctgcgctcgg tagtcggtca
      781 gcacgagatg gacgacctgc tctcgcagcg tgacaagctc aacgtcgacc tgcagcgcat
      841 tctcgatcag agcaccgagg cctggggcat caaggtttcg acggtcgaga tcaaggacgt
      901 cgacctcgat gagaccatga tccgcgccat ggcgaagcag gccgaggccg agcgcacgcg
      961 gcgtgccaag atcatcaacg ccgagggcgt gaagcaggcg gcgcagactc tggtcgaggc
     1021 ggcgcaggcg ctggcggcgg cgccgagcgc actgcagctg cgttacctgc agactctcgc
     1081 cgacatcgcc catgagcgca cgcaactgat cgtcttcccg ctgccgctcg atctgatcaa
     1141 gccgctgctc ggcccacgcg gtgactaggg caccccgcga aatttcgctc accgcgctcg
     1201 gcaagtgccc ctcgaccggc aagaagcgaa atctcgcggc gaccccgctg gcacccgtgc
     1261 gctcgcgctc gccggactgc tgctggcgct cgccaccgtc tgtggcgcgt tcggcgcgca
     1321 cgcgctcaag agtcaactcg cgcccgagcg gctgcagttg tgggacacgg cggtgcgtta
     1381 ccagttcttc caggcgctgg gactgctcgg gatcgggctg accctgcgtc ttctcgagcc
     1441 ggccggcggt gtcgctgcgg gagcgctgcg tgccgccgcc gtgttgagcg ccgccggagt
     1501 gttgctgttc tcgggaagtc tgtacgcgct cgccctcggc gcttcacgcg ggctcggcgt
     1561 gctcacgccg ctcggcggca tcgcgtggat tgccgcgtgg ctgctatttg cctggggtgc
     1621 gtggcgcgcg caatggtgag atcggaaatg gagttgcctg catgtcgact cgcaccctgt
     1681 ctgcggccct gatcgcggct ggcgccatgg cgctggcctt cgtcgctacc ccatacatct
     1741 ccgccgccgc cgcggccgct gccggtcacg cgcagctcgg cagctttggc ctcgatctct
     1801 cgggcggaga tccgagcgtc aagcccggcg atgacttcgc gcgctacgcc aacggtcgct
     1861 ggctggacac ggcacagatt ccgcccgacc gcgcctcctg gggcagcttc gccatgctgc
     1921 gcgagcgctc gttgcagcag gtgcgggaca ttctcgaggc gctccccaat gagagcccgc
     1981 aaggcagcaa cgagcagaag ctccacgact tctaccgctc ctacctcgat accgacgcga
     2041 tcgagcgcgc cgggctgagc tccgcgaaag ccgtg
//
LOCUS       DATDMP010000205         5893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_475497, whole genome shotgun sequence.
ACCESSION   DATDMP010000205 DATDMP010000000
VERSION     DATDMP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475497"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..969
                     /gene="dapA"
                     /locus_tag="VI195_03465"
     CDS             67..969
                     /gene="dapA"
                     /locus_tag="VI195_03465"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY6823472.1"
                     /translation="MFAGSLVAIVTPMRADGAIDFEAWAGLVELHAAQGTDGIVVGGT
                     TGESATLQDAELRELLLRACAQLQGRLTLIAGAGTSGTASTVERVAWLSELPIDGLLV
                     VTPAYNRPSQEGLYRHFAAAAAAARKPLVLYNVPARTAVDMQPATVARLARVAGIVGI
                     KEAVAEPARVRELVALCGQDFVVLSGDDASARASIAAGARGVISVTANVAPRAMSQML
                     AAVRHGDVARAAELDAPLSGLHRELFVEANPIPVKWLLAQMGLIGPGIRLPLTPLAAE
                     HHAGLLAAARTAGVPVRESVVHEE"
     gene            959..1282
                     /locus_tag="VI195_03470"
     CDS             959..1282
                     /locus_tag="VI195_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823473.1"
                     /translation="MRNSDEECVMRGARAVGLFLLLLPVMSGCHTFSHATEKSCHGPQ
                     QYQKAQSVPPLKVPTGLDAPDTANALRLPALHDPAPPPRKGTQPCLDEPPPFKVTQPA
                     KTPQA"
     gene            1323..1414
                     /locus_tag="VI195_03475"
     tRNA            1323..1414
                     /locus_tag="VI195_03475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Ser,seq:gga)
     gene            complement(1472..2245)
                     /locus_tag="VI195_03480"
     CDS             complement(1472..2245)
                     /locus_tag="VI195_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823474.1"
                     /translation="MAVAKVADGVASKVPEVTLGFWIIKVAATTLGETGGDTLSMTLG
                     LGYLASTLIFATALVLLAACQIAAKRFHPFLYWATIIASTTAGTTLADFADRSLGIGY
                     AGGTLVLLACVLGSLGLWYRTEGTVSVNTVNTARVEAFYWLTITFSQTLGTALGDWVA
                     DAGLGYRGGALVFGALLILVALAYYRTRISHVTLFWTAFILTRPLGATVGDFFDKPIT
                     QGGLNVSRPLASAIIAVFILVCILLIPQRAGSHPRSAGG"
     gene            2401..2498
                     /gene="ffs"
                     /locus_tag="VI195_03485"
     ncRNA           2401..2498
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VI195_03485"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2537..4135
                     /gene="dnaX"
                     /locus_tag="VI195_03490"
     CDS             2537..4135
                     /gene="dnaX"
                     /locus_tag="VI195_03490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY6823475.1"
                     /translation="MSYTALARKYRPKKFSELVGQEHVRRALVNALTSGRVHHAFLFT
                     GTRGVGKTTIARILAKCLNCETGITAEPCGVCASCREIDAGRFPDLLEVDAASRTKVD
                     DTRELLDNVQYAPARGRYKVYLIDEVHMLSAHSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFNLKRLPSADIAAHLHTILSAEGVPFDPAAVTLIAHAADGSLRDAL
                     SLLDQLIAFGGGRAGEAEARAMLGTVAHDRVVRILELLARGERAELLRCAQSLEEFAP
                     DYAQVLDALAGLLVRVGLHQAVPGYEGDELYAAEVLERLAAELSAEDVQLFYQTAIIG
                     RRDLPLAPAPASGFAMTLLRMIAFRPAEAAQELRTAGAVGPQASPRASSAGQGGAAGT
                     PGATASAAGPPATGEGAWAAIVAQLELTGAARQLATHCVFLGRQGVMVRLGLDPRHQL
                     VRTLATEEKLAQALSRHFGSTVRLEFHSGTAQGAETPAQAQRRASDAELAAARRAFEE
                     DAGVKSLRERFGATVLPDTVRPVK"
     gene            4186..4509
                     /locus_tag="VI195_03495"
     CDS             4186..4509
                     /locus_tag="VI195_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004642711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY6823476.1"
                     /translation="MRGNFNNLMKQAQAMQANMQKAQAEIASIEVIGEAGGGMVKVTM
                     NGRHEVKRVQIEPTVSAEDREMLEDLIAAAANDAVHKVEARVQEKMASITAGLQLPPG
                     MKLPF"
     gene            4520..5116
                     /gene="recR"
                     /locus_tag="VI195_03500"
     CDS             4520..5116
                     /gene="recR"
                     /locus_tag="VI195_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY6823477.1"
                     /translation="MKHSPRLAALIEALRTLPGVGPKTAQRMAFHLLQEGRPGARTLA
                     QALAAALESVARCRRCRMLTEGELCVICAAPQREVALLCAVESPADVVAIESSGSYRG
                     RYFVLMGHLSPLDGIGPEQLGVRELESILAEGEVRELILATNSTVEGEATAHYLSELA
                     SRRGVRASRIAQGVPLGGELEYVDGGTLAHALAGRQSL"
     gene            5250..5822
                     /locus_tag="VI195_03505"
     CDS             5250..5822
                     /locus_tag="VI195_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY6823478.1"
                     /translation="MKTKNAHSLTARIWTFVLLSLGLALGPIALALDTAALDKEVAAT
                     LEQFYAMNAENKGLVSSAAGVLVFPSITKAGVGVGGLHGDGALLENGKTVGYYSTSAA
                     SVGLTLGVARHSEVILFKTPEALNKFVNSKNWSIGADASVAVVKTGAGGSYDTQTLSK
                     PVLAFVFGEKGLMGDVSLKGAKINKLDTSK"
BASE COUNT          976 a         1905 c         2055 g          957 t
ORIGIN      
        1 aacttccgcc cgtaacatcg cacgcttccc accgcaagac acgacgccag cagcgattcc
       61 cagcctatgt ttgccggcag cctggttgcc attgtcaccc ccatgcgcgc tgatggcgcg
      121 atcgacttcg aggcctgggc agggctggtc gagctgcacg ccgctcaggg gacggacggt
      181 atcgtcgtcg gcggcacgac cggtgagtcg gcaacgctgc aagatgcgga gctgcgcgag
      241 ctgctgctgc gcgcttgcgc gcagttgcag gggcggctga cgctcatcgc gggtgccggc
      301 acgagcggca cggcttcgac ggtggagcgg gtcgcgtggc tctcggagct gccgatcgat
      361 gggctgctgg tcgtcacccc ggcctacaac cgcccatcgc aagaggggct gtatcgacac
      421 ttcgcggctg cggctgccgc ggcgcgcaag ccgctggtgc tgtacaacgt gccggcacgc
      481 acggcggtcg acatgcagcc ggccacggtg gcgcgcctcg cgcgcgtcgc cgggatcgtc
      541 ggcatcaagg aggcggtcgc cgagcccgcg cgggtgcgcg agttggtcgc gctctgcggg
      601 caggatttcg tggtgctgag cggcgatgat gccagcgcgc gcgcgagcat cgcggcgggg
      661 gcgcgcggag tgatctcggt gacggcgaac gttgcgccgc gcgcgatgtc acagatgctg
      721 gccgccgtgc ggcacggcga tgtggcgcgc gccgccgagc tcgatgcccc cctgagcggc
      781 ctgcatcggg agctgttcgt cgaggcgaac ccgatcccgg tgaagtggct gctggcgcag
      841 atgggtctga tcggtcccgg gatccgattg ccgctcacgc cgctcgcggc ggaacaccac
      901 gccggcctgc tggcggcggc gcggactgcc ggagtacctg tacgagagag cgtagtgcat
      961 gaggaatagc gatgaggagt gcgtgatgcg aggtgcgcgg gcggttggcc tgttcctcct
     1021 gctgctgccg gtcatgagcg gttgtcatac gttcagccat gccaccgaga agtcctgtca
     1081 cggcccgcag cagtaccaga aggcccagag cgtgccgccg ctgaaggtcc ccaccgggct
     1141 cgacgcaccc gacacggcca acgcgctgcg cctgccggct ctgcacgatc cagcgcctcc
     1201 gccgagaaag ggcacccagc cctgtctcga tgagccccca ccgttcaagg tgacgcagcc
     1261 cgccaaaacc ccccaggcct gaagcggagt ggcttatcat gcgccgcctt aactgattga
     1321 tcggagaggt ggccgagcgg tcgaaggcgc tggactggaa ttccagtaac atctcaacgg
     1381 gtgttcgtgg gttcgaatcc caccctctcc gccagttgct ttgcaggcgg tggccgctat
     1441 tggccaaaga cctaccaaga gcggtcgggc ctcacccgcc ggcgctcctc ggatggctgc
     1501 ccgctctctg cgggatgagc agtatgcaaa ctaggatgaa caccgcgatg atcgcgctgg
     1561 caagcggccg gctcacgttg aggcctccct gggtgatcgg cttgtcgaag aaatcgccca
     1621 cggtggcgcc gagcggccgc gtgagaatga acgcagtcca gaagagtgtg acgtgcgaga
     1681 tgcgagtgcg gtagtaggcg agggcgacca ggatcagcag cgcaccgaag accagcgcgc
     1741 cgccgcggta gccgagtccc gcatcggcga cccagtcacc gagcgcggta ccgagcgtct
     1801 gcgagaaggt gatggtgagc cagtaaaagg cctccacacg cgctgtgttg acggtgttga
     1861 ccgacactgt gccttcggtg cggtaccaga gaccaaggga gccgagcaca caggcgagca
     1921 gcacgagtgt gccgcccgca tagccgatgc cgagcgagcg atcggcaaag tcggcaagcg
     1981 tggtgccggc ggtggtcgag gcgatgatcg ttgcccagta gaggaacgga tggaaccttt
     2041 tcgccgcgat ctgacaggcc gcgagcagca ccagtgcggt ggcgaagatc agcgtgctgg
     2101 cgaggtagcc gagaccgagc gtcatcgaca gggtatcgcc cccggtctcg cccagcgtgg
     2161 tggctgcgac cttgatgatc cagaagccga gggtgacctc ggggaccttg ctggcaacgc
     2221 catccgcaac cttggcgacg gccacgtctc gcctcatgca gactcactcg agttggtgcc
     2281 aggcgattgt gcctgttttc catggcggtg cgcggtggtg atgaccacca cgcggcgcag
     2341 ccggcggtcg agctcacctc atgtgatagc atcgagcgct tttcgcgata cggtggcccg
     2401 catcggtttc tccgcgacga ccagtcgtga atcccgtcag ggccggaagg cagcagcggc
     2461 agcggcgatg tcgggtgccg gagctcaagc tggtgcgggc cgcctcctgt cgcttggcaa
     2521 ctaacccggg acttgcatga gctacaccgc gctggcgcgc aaatatcgtc cgaagaaatt
     2581 ctccgagctc gtcggacagg agcacgtgcg gcgcgcactg gtcaatgcgc tcacgagcgg
     2641 acgcgtgcac catgccttcc ttttcaccgg cacgcgcggc gtaggcaaga ccacgatcgc
     2701 gcgcatcctg gcgaagtgcc tcaactgcga gaccgggatc actgccgagc cctgtggggt
     2761 gtgcgccagt tgccgcgaaa tcgatgccgg ccgctttccg gacctgctcg aggtcgacgc
     2821 cgcctcacgc accaaggtgg acgacacgcg cgagctgctc gataacgtgc agtacgctcc
     2881 ggcgcgtgga cgctacaagg tgtacctcat cgatgaggtg cacatgctct cggcgcactc
     2941 cttcaacgcc ttgctgaaga cgctcgagga gccaccgccg cacgtcaagt tcctgctggc
     3001 cactaccgac ccacagaagc tgccggttac ggtgctctcg cgctgcctgc agttcaatct
     3061 gaagcggctg ccgtcggcag atatcgctgc tcatctgcac accatactga gcgccgaagg
     3121 tgtgccattc gatccggccg ccgtgaccct catcgcgcac gcggcggatg gcagcctgcg
     3181 cgatgccctc tcactgctcg accagctgat cgccttcggc ggcggccgcg cgggcgaggc
     3241 cgaggcccgt gcgatgctcg gcaccgtggc gcacgatcgt gtcgtgcgca tcctcgagct
     3301 gctcgcgcgc ggcgagcgtg cggagctgct gcgctgcgcg cagtcgctgg aggagttcgc
     3361 tccggattac gcccaggttc tcgatgcact cgcagggctg ctggtgcgtg tcgggttgca
     3421 tcaggcggtt cccggctacg agggcgatga gctctacgca gcggaggtgc tcgagcggct
     3481 cgccgccgag ctctctgccg aggacgtgca gctcttctat cagaccgcga tcatcggtcg
     3541 tcgcgatctc ccgctcgcac ccgcgccggc gagtggcttc gccatgacgc tgctgcggat
     3601 gatcgcgttc cgtccggccg aggcggcgca ggagctgcgc acggccggcg cagtggggcc
     3661 gcaggctagt cctcgagcca gctcggccgg tcagggcggc gctgcgggca cccccggcgc
     3721 aaccgcttcg gccgccggtc cacctgccac gggggagggc gcctgggcgg caatcgtggc
     3781 ccagctcgag ctgaccggcg cggcgcggca gctcgcgacc cactgcgtgt ttctcggacg
     3841 ccagggcgtg atggtgcgcc tcgggctcga tccgcgtcac cagctggtgc gaaccctggc
     3901 caccgaggag aagcttgcgc aggcgctgtc gcggcatttc ggctcgacgg tgcggctcga
     3961 gttccactcg gggacggcgc agggcgccga gacgcccgcg caggcacagc ggcgcgccag
     4021 cgacgcggag ctcgccgccg cgcgccgtgc gttcgaggaa gatgcgggcg tcaagagcct
     4081 gcgggagcgc ttcggggcca cggtgctacc ggacacggtc cgcccggtaa aatgaaccct
     4141 tggagtttca gcggcgccgc ggcgcgccac aggtgagtca gtccaatgcg gggtaacttc
     4201 aacaatctga tgaagcaggc ccaggccatg caggcgaaca tgcagaaggc ccaggccgag
     4261 atagccagca tcgaggtgat cggcgaggcg ggcggcggca tggtgaaggt caccatgaac
     4321 ggccgccacg aggtgaagcg ggtgcagatc gagccgacgg tgagcgcgga ggaccgcgag
     4381 atgctcgagg acctgatcgc cgccgccgcc aacgatgccg tacacaaggt cgaggcgcgc
     4441 gtgcaggaga agatggcgag catcactgcg gggctgcagc tcccgccggg aatgaagctg
     4501 ccgttctgag cgcgctgcga tgaagcattc accgcgcctc gcggcgctca tcgaggcatt
     4561 gcgtacgctg ccgggtgtcg gacccaagac tgcgcagcgc atggccttcc acttgttgca
     4621 ggaggggcgg ccgggcgcgc gtacgctcgc gcaggcactt gccgcagcgc tcgagtcggt
     4681 ggcgcgctgt cggcgctgtc gcatgttgac cgagggtgag ttgtgcgtga tctgcgcggc
     4741 accgcagcgt gaggtggcgc tgctatgcgc ggtcgagtcg cccgccgatg tggtggcgat
     4801 cgaatcctcc ggcagctacc gcggacgcta tttcgtcctc atgggccacc tctcgccact
     4861 cgatggcatc ggtcccgagc agctcggcgt gcgcgagctc gagtcgattc tcgccgaggg
     4921 cgaggtgcgt gagctcattc tcgccaccaa ctcgaccgtc gagggagagg cgaccgcgca
     4981 ctacctgagc gagctcgcca gccgccgtgg cgtgcgcgcc agccgtatcg cccagggtgt
     5041 gccactcggc ggggagctcg agtacgtcga cggcggcacg ctcgcgcacg cgcttgctgg
     5101 caggcagtcg ctttgaggga caaacgtgag cgtggctgga agcttcgcag ctcgctgctc
     5161 acctaactga cttccaactg cttgttgagt aactacccgc gcaacgtgca cacttggcgt
     5221 cacatgttgt ttgtcacgga gagttgtgca tgaagaccaa gaacgctcat tcgctcacag
     5281 caaggatctg gactttcgtc ctgttatctc tcggcctcgc gctcggcccg atcgcgctgg
     5341 cactcgatac cgccgcactc gacaaggagg tggccgcgac gctcgagcaa ttctacgcca
     5401 tgaacgcaga gaacaagggc ctggtcagca gtgccgccgg agtgctggtg tttcctagca
     5461 tcaccaaagc cggagtcggc gtgggcggcc ttcatggcga tggcgcgctg ctcgagaacg
     5521 gtaagaccgt gggctactac tcgacctcgg ccgcctcggt gggcttgacc ctcggggtcg
     5581 cgcgtcacag cgaggtgatc ctgttcaaga cgccggaggc gctgaacaag ttcgtcaaca
     5641 gcaagaactg gtcgatcggt gcggatgcct cggtcgcggt cgtcaagacc ggtgccggcg
     5701 ggagctacga cacccagacg ctcagcaagc cggtgctcgc cttcgtgttc ggcgagaagg
     5761 gtttgatggg cgatgtctcg ctcaagggcg ccaagatcaa caagctcgat acgagcaaat
     5821 agcggacccg gaacggcgat gagccgagtc gccgccggct cggctcatct gctcgttcga
     5881 gtgcactagc tcg
//
LOCUS       DATDMP010000206         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_698164, whole genome shotgun sequence.
ACCESSION   DATDMP010000206 DATDMP010000000
VERSION     DATDMP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698164"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VI195_03510"
     CDS             complement(<1..1160)
                     /locus_tag="VI195_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6823479.1"
                     /translation="MNEALRTVRGGRIDRSRPLPFRFDGVRYAGFAGDTLASALLANG
                     VHLLGRSFKYHRPRGVLAAGAEEPNALVGVRRDAARYTPNIRATQLELYAGLEAESQN
                     RWPTLAFDVGALNDLIAPFIPAGFYYKTFMWPRRAWKSLYEPRIRAAAGLGCAPRLAD
                     PDRYATRYAHCDLLVVGGGPAGLAAADAAASAGARVMLCDEQSEFGGALLSQNPQDAP
                     RLDDRPAPQWLAGILAALAANPRVTLLPRTTAFGYFPHNLVSLNERVTDHLPVPPPEW
                     PRERRWLVRAREVVLATGAIERPLIFPGNDRPGIMLAGAARAFLNRYGVRAGTRAVLV
                     TATDEAYAVALELQRAGIFVATIADVRAGESAAVSGVRAARIPVLTGATVLGT"
     gene            complement(1157..1447)
                     /locus_tag="VI195_03515"
     CDS             complement(1157..1447)
                     /locus_tag="VI195_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY6823480.1"
                     /translation="MLLIDCPYCGERPELEFVYGGQAHIARPRDPAQVDAQQWTRFLY
                     LRNNLRGIHAERWRHAHGCGRFFNALRDTTTDHFLATYKVGEPAPPVAKESR"
     gene            complement(1462..>2464)
                     /locus_tag="VI195_03520"
     CDS             complement(1462..>2464)
                     /locus_tag="VI195_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY6823481.1"
                     /translation="YLFPESAALYDHAVKLWESLSQTLNYNVMFSQRGVMMLAHTVHD
                     VQVAKRHVHANRAAGVDNEWLTPQQARAFCPPLNIGDIRYPVLGAALQRRGGTARHDA
                     VAWGYARAADALGVDIIENCEVTGIRRNADGAVAGVETTRGTIRAPRIGVAAAGHTSV
                     ILRMADVELPLESYPLQALVSEPVKPVFPCVVMSNTIHAYISQSDKGELVIGAGTDAY
                     TSYTQRGGPHITSHALEAICELFPMFRRLRMLRCWGGIVDVTPDRSPIIGKTGVPGLY
                     VNCGWGTGGFKATPGSAHVFAWTIAHDEPHPLNAPFTLERFGDGSLIDEAAAAAVAH"
BASE COUNT          412 a          822 c          863 g          367 t
ORIGIN      
        1 gtcccaagca ccgtggcgcc ggtgagcacg gggatgcgcg ccgcgcgcac gccgctcacg
       61 gccgcgctct ccccggcgcg tacgtccgcg atggtggcga cgaagatccc tgcccgctgc
      121 agctcgagtg ccaccgcata cgcttcgtca gtcgccgtga cgagcaccgc acgtgtgccc
      181 gcgcgcacac catagcgatt gaggaaggcg cgcgcggcac ccgcgagcat gattccgggc
      241 cgatcattgc ccgggaagat gagcggacgc tcgatcgctc cggtcgcgag caccacctcg
      301 cgcgcgcgca ccagccaccg gcgctcgcgc ggccactccg gcggcggcac cggcaggtga
      361 tcggtgacgc gctcgttgag gctcaccagg ttgtgtggaa agtacccgaa ggcggtggtg
      421 cgcgggagca gtgtcacgcg cgggtttgcg gcaagagcgg ccagaatgcc ggcgagccac
      481 tgcggtgcgg gacggtcatc gagtcgcgga gcatcctgcg ggttctgtga gagaagtgcg
      541 ccaccgaact cggactgctc atcgcagagc attacgcgtg cacccgccga agcggctgca
      601 tcagcggcgg cgagcccggc gggaccgccg ccgacgacca gcagatcgca atgcgcgtag
      661 cgcgtggcgt agcgatcggg atccgccaga cgcggcgcgc acccgaggcc cgcggcggcg
      721 cggatgcgtg gctcgtacag cgatttccac gcccggcgcg gccacatgaa cgtcttgtag
      781 tagaagcccg ccgggatgaa cggggcgatc aggtcattga gcgcacccac atcgaacgcg
      841 agggtcggcc agcggttctg actctccgcc tcgaggccgg cgtagagctc gagctgcgtc
      901 gcacggatat tcggcgtata gcgcgccgca tcacggcgca ctccgacgag tgcattcggc
      961 tcctccgctc ccgcggccag tacgccgcgg ggccggtgat acttgaagga gcgaccgagc
     1021 agatgcacgc cgttggcgag aagcgccgag gcaagcgtgt cgcccgcaaa gcctgcatag
     1081 cgcaccccat cgaacctgaa ggggagcgga cgcgagcggt cgatgcgccc gccgcgcacc
     1141 gtgcgcagcg cctcattcac cgcgactcct tggccaccgg cggcgctggc tcgcccacct
     1201 tgtaagtcgc aaggaagtga tcggtggtgg tatcgcgcag cgcgttgaag aagcggccac
     1261 agccgtgggc atggcgccag cgctcggcgt ggatgccgcg caggttgttg cgcaggtaaa
     1321 gaaagcgggt ccactgctgc gcgtctacct gcgccggatc gcgcgggcgg gcgatatgcg
     1381 cctgcccgcc atagacgaat tcgagctccg ggcgctcgcc gcagtaggga caatcgatga
     1441 gcagcatggc ccggtgctcc ttcagtgcgc gaccgccgca gccgctgcct catcgatcag
     1501 gctgccgtcg ccgaagcgct cgagcgtgaa gggggcgttg agcggatgcg gctcatcgtg
     1561 cgcaatcgtc cacgcgaaca catgcgccga gcccggtgtc gccttgaagc ccccggtgcc
     1621 ccagccgcaa ttcacgtaca gtcccggcac gccggtcttg ccgatgatgg gtgagcggtc
     1681 cggggtcaca tcgacgatgc cgccccagca gcgcagcatg cgcaggcgac ggaacatggg
     1741 aaagagctcg cagatcgcct cgagcgcgtg gctggtgatg tgcgggccgc cgcgctgcgt
     1801 gtaagaggtg taggcatcgg ttccggcgcc gatcaccagc tcgcccttat ccgactggct
     1861 gatgtaggcg tgaatggtgt tggacatgac gacgcacgga aacaccggct tcacgggttc
     1921 cgagacgagc gcctgcagcg gatagctctc gagcggcagc tccacatccg ccatgcgcag
     1981 gatcacgctg gtgtgtccgg ctgccgccac gccgatgcgc ggtgcgcgaa tcgtgccgcg
     2041 agtggtctcg acaccggcca cggcgccgtc cgcgttgcgc cggatacccg tcacttcgca
     2101 gttctcgatg atatcgacgc cgagcgcatc ggcggcacgc gcataccccc aggccacggc
     2161 gtcgtgacgc gcggtgccgc cgcggcgttg cagcgcggcg ccgagcaccg gatagcggat
     2221 gtcgccgata ttcaaaggtg ggcagaaggc ccgggcttgc tgcggtgtga gccactcgtt
     2281 gtcgacgccg gcggcgcgat tggcgtgcac gtggcgtttc gccacctgca catcgtgcac
     2341 ggtgtgcgcc agcatcatca caccgcgttg cgagaacatg acgttgtagt tgagcgtctg
     2401 cgacagactc tcccaaagct tcacggcgtg gtcgtagagc gcggcacttt ccggaaacag
     2461 atag
//
LOCUS       DATDMP010000207         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1586662, whole genome shotgun sequence.
ACCESSION   DATDMP010000207 DATDMP010000000
VERSION     DATDMP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586662"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VI195_03525"
     CDS             complement(<1..425)
                     /locus_tag="VI195_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6823482.1"
                     /translation="MLTHLQIRDFAIIDAVELELRPGLTVLTGETGAGKSILVDALQL
                     LAGGRAGAEVVRHGAERAEIAATFELSHAPRELKPWLEEHSLAGGPELAVRRVIGSDG
                     RSRAYLNGQSVPVQLLREAGSILIDIHGQHEFQSLTRGAA"
     gene            complement(428..1312)
                     /locus_tag="VI195_03530"
     CDS             complement(428..1312)
                     /locus_tag="VI195_03530"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEY6823483.1"
                     /translation="MAFPVRTCALVGRFSDARVAESLGTLLPHLQSRGVRVLVSEDAE
                     LAREAPVTRVPERTLGERADLVIAVGGDGTLLHAARLVARHNVPLLGVNRGRLGFLTD
                     VMPQDMLPSVDAALAGQLEADERPLLTARLHRPGAEVAQSFALNDVVMQKHDTGRTLD
                     FETRIDGSYVNRHDGDGIIVASPTGSTAYALSCSGPIIEPHLPALVIVPICAHTLSDR
                     PIVVAANCVIEVLLLERAGTQANVTCDGALLGALTPADRLEVTTAAERVTLLHPPGHD
                     YYRLLRSKLRWGRGSFEH"
     gene            1471..2529
                     /gene="hrcA"
                     /locus_tag="VI195_03535"
     CDS             1471..2529
                     /gene="hrcA"
                     /locus_tag="VI195_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY6823484.1"
                     /translation="MAHEGRDEQLGDRAQQLLRTLIESYIRDGQPVGSRVLSRESGLQ
                     LSAATIRNVMADLEELGFVASPHTSAGRVPTDKGYRFFVDTLLQLRPPDDAATAEMRR
                     QFEASRDSSTELIATVSQLLSSATQLAGVVTLPRTRQAAVTQMEFVGLSENRVLVVLV
                     FNDREVQNRIIQLERHYSVDELKRASNYLNEQFRGRSVREVRQELLRQLSEARAHMNQ
                     IMLDAISVAQQVFEAGSEDRLEYVIKGETNLMGMAELTNVEKLRRLFEAFNEKRDFLH
                     LLDQSLKAEGVQIFIGHESGYRILDDCSVVTAPYADGDAVVGVLGVIGPTRMAYERVI
                     PIVDMTAKLLGAALNSRR"
BASE COUNT          418 a          891 c          879 g          396 t
ORIGIN      
        1 gccgcgccgc gggtgagcga ctggaattcg tgctgtccgt ggatgtcgat gaggatgctg
       61 ccggcctcgc gcaacagctg caccggtacc gattgcccgt tgaggtaggc gcgcgagcgg
      121 ccgtcgctgc cgatcacgcg gcgcaccgcc agctcggggc cgccggcgag cgagtgttcc
      181 tcgagccatg gcttgagctc gcgcggcgca tggctgagct cgaaggttgc agcgatctcg
      241 gcacgctcgg cgccgtgccg taccacttcc gcgccggcgc gcccgccggc gagcagctgc
      301 agcgcgtcga cgaggatcga cttgccggcc ccggtttcgc cggtgagcac cgtgagcccg
      361 ggacgcagct cgagctccac cgcatcgatg atggcgaagt cgcgtatctg caggtgtgtc
      421 agcatgctca gtgctcgaag ctgcctcggc cccagcggag cttcgagcgc agcagccggt
      481 agtaatcatg accgggcgga tgcagcagcg tcacgcgctc ggccgccgtg gtcacctcga
      541 gacggtcggc gggagtgagc gcgccgagca gcgcgccatc gcaggtgacg ttcgcctgcg
      601 taccggcgcg ctcgagcagc agcacctcga tcacgcagtt cgccgccacg acgatcggcc
      661 ggtcggagag ggtgtgcgcg cagatgggta cgatgacgag cgcgggcagg tgcggctcga
      721 tgatcggacc cgagcaggag agggcatagg cggtcgaacc cgtcgggctc gccacgatga
      781 tgccatcgcc gtcgtgccta ttgacgtagc tgccatcgat gcgcgtctcg aagtcgagcg
      841 tgcgcccggt gtcgtgcttc tgcatgacga catcgttcag cgcaaaggat tgcgccacct
      901 ccgcgcccgg ccggtggagg cgggccgtga gcagcggccg ctcgtccgct tcgagctggc
      961 cggcgagcgc cgcgtccacc gacggcagca tgtcctgagg catgacgtcg gtgaggaagc
     1021 cgaggcggcc gcggttgacg cccagcagcg gcacgttgtg ccgcgcaacg aggcgcgcgg
     1081 catggagcag ggtgccgtct ccgcctaccg cgatcaccag gtccgcgcgc tcacccaggg
     1141 tgcgctccgg cacgcgcgtg accggcgcct cgcgcgcgag ctccgcatct tcgctcacca
     1201 gcacacgcac cccccggctc tgcaggtgtg gcagcagcgt gccgaggctc tcggcgaccc
     1261 tcgcatccga gaatcggccg accaacgcac aggtacgcac cggaaaagcc atgtgcctcg
     1321 attgaacttc acctagccgc aaggcgcgcc tttttagcac gcggggcgcg ccgggagcca
     1381 atgccgccgg tcacatatcg ttaggataag cacgccggcc gcggccgttg cttgacggtc
     1441 tctcggcccc gaagcaagct ttcctgctgt atggcgcacg aaggccggga cgagcagctc
     1501 ggggaccgtg cgcagcagct gctgcgcacg ctcatcgaga gctacatacg cgacggtcag
     1561 ccggtcggct cgcgggtgct ctcgcgcgaa tcgggactgc agctgtcggc ggcaacgata
     1621 cgcaacgtga tggcggacct ggaggagctc gggttcgttg cctcacccca cacctccgcc
     1681 gggcgggtgc cgaccgacaa gggctaccgc ttcttcgtcg acacgctgct gcagctgcgc
     1741 ccccccgatg acgcggcgac ggccgagatg cgccgccagt tcgaggcgag ccgcgacagc
     1801 tcgaccgagc tcatcgccac cgtctcccag ctgctctcct cggcgacgca gctcgccggg
     1861 gtcgtcacgc tgccgcgcac gcggcaggcc gcagtcacgc agatggagtt cgtcggactt
     1921 tccgagaacc gcgtgctggt ggtgctggtg ttcaacgatc gcgaggttca gaaccgcatc
     1981 atccagctcg agcggcatta ctccgtcgac gagctcaagc gcgcttccaa ctacctgaac
     2041 gagcagttcc gcggccgctc ggtgcgcgag gtgcgccagg agctgctgcg gcagctctcc
     2101 gaggcacgcg cgcacatgaa ccagatcatg ctggatgcca tctccgtcgc gcagcaggtg
     2161 ttcgaggccg ggagcgagga tcggctcgag tacgtcatca agggcgagac caacctcatg
     2221 ggaatggccg agctcaccaa cgtcgagaag ctgcgccgcc tcttcgaggc gttcaacgag
     2281 aagcgcgact tcctgcacct gctcgatcag agcctcaagg ccgaaggggt gcagatcttc
     2341 atcggtcacg agtccggcta ccgcatcctc gatgactgca gcgtggtcac cgccccctac
     2401 gccgacggcg atgccgtcgt cggggtgctc ggggtcatcg gacccacccg catggcctac
     2461 gagcgagtca tcccgattgt cgacatgacc gctaaactgc tcggcgccgc cttgaattcg
     2521 cgccgttgac ccccaccccc ggggtgcccg aaccgagcga gcggcgaaga gccgactgcg
     2581 cctt
//
LOCUS       DATDMP010000208         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1364902, whole genome shotgun sequence.
ACCESSION   DATDMP010000208 DATDMP010000000
VERSION     DATDMP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364902"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..771)
                     /locus_tag="VI195_03540"
     CDS             complement(67..771)
                     /locus_tag="VI195_03540"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY6823485.1"
                     /translation="MRRFRIYRFDPDSGANPRLDTFELEVKACGPMVLDALIQIKAAI
                     DSSLTFRRSCREGICGSCAMNINGVNCLACTTSMQDLSAEVRIYPLPHLPVVKDLVAD
                     LTQFYAGYAAVKPWLRARTPAPPDHERLQSKEEQERIERPAGCILCACCSTGCPSYWW
                     NSDRYLGPAALLAAYRWIIDSRDEATGERLDELEDPFRLYRCHTIMNCTDVCPKDLNP
                     GKAIAEIKKLLIARQS"
     gene            complement(858..>2083)
                     /locus_tag="VI195_03545"
     CDS             complement(858..>2083)
                     /locus_tag="VI195_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY6823486.1"
                     /translation="ADGTLHRFRAHVTILATGGYGRTYFSCTSAHSCTGDGNAMVLRA
                     GLALQDMEFVQFHPTGIYGAGCLITEGARGEGGYLTNSAGERFMERYAPNAKDLASRD
                     VVSRAMTVEIREGRGVGVEHDHLELHLEHLGPGVIHERLPGIAETARIFAGVDVTREP
                     IPVQPTVHYNMGGIPCNHHGEVVCPKEGDPDRVVPGLMAVGEAACVSVHGANRLGSNS
                     LLDLVVFGRAAARRCAATIVPASPHAPLPAGAGEQAVARLDRLRNANGARGTAAIRME
                     MQKTMQLDAAVFRTGATLAQGIERIAQTFESFADVRIQDRSLIWNTDLIETLELENLL
                     FQASATIHSAAHRSESRGAHAREDFPQRDDRNWLKHTLVWVEGPRRTRFDYRPVHLHT
                     LTGEVESIPPRPRTY"
BASE COUNT          303 a          702 c          757 g          321 t
ORIGIN      
        1 gctccttcag gccgcgccgg cagcgccacg tgaggcggcg cagctccgct cccggagcgc
       61 cgccgctcag ctctggcgtg cgatgagcag cttcttgatc tcggcaatcg ccttgccggg
      121 attgaggtcc ttggggcata cgtcagtgca gttcatgatc gtgtggcagc gatacaggcg
      181 aaacggatcc tcgagctcat cgagccgctc gccggtggcc tcgtcgcggc tgtcgatgat
      241 ccagcggtag gcggcgagca gcgccgcggg gccgaggtag cgatcgctgt tccaccagta
      301 gctcgggcag ccggtcgagc agcaggcgca caggatgcag ccggcgggcc gctcgatgcg
      361 ctcctgttcc tccttgctct gcagccgctc gtgatccggc ggcgcgggcg tgcgcgcgcg
      421 cagccacggc ttcaccgccg cgtagcccgc gtagaactgc gtgaggtcgg cgaccaggtc
      481 cttcaccacc ggcaggtgcg gcagcggata gatgcgcacc tcggcgctga gatcctgcat
      541 cgaggtcgtg caggcgagac aattcacgcc gttgatgttc atggcgcacg agccgcagat
      601 gccctcgcgg caggagcggc ggaaggtgag cgaggaatct atcgccgcct ttatctggat
      661 gagcgcatcg agcaccatcg ggccgcaggc cttcacctcg agctcgaagg tgtcgaggcg
      721 cggatttgcg ccgctatcgg gatcgaagcg gtagatgcgg aagcggcgca ccgcccccgc
      781 accgctgcgc gagcggtggg tgacacccgg cacgatgcgc gagttggcgg gcaggcggaa
      841 ttgggccatg gcgatcctca gtaggtgcgc ggccggggcg ggatcgattc gacctccccg
      901 gtgagcgtgt gcagatgcac cggacggtag tcgaagcgcg tacgccgcgg gccctcgacc
      961 cacaccagcg tgtgcttgag ccagttgcgg tcgtcgcgct gcgggaagtc ctcgcgcgcg
     1021 tgcgcgccgc ggctctcgct gcggtgcgcc gccgagtgga tcgtggcgct cgcctggaac
     1081 agcaggttct cgagctcgag cgtctcgatg agatcggtgt tccagatgag cgagcggtcc
     1141 tggatgcgca cgtcggcgaa ggactcgaag gtctgcgcga tgcgctcgat accctgcgcg
     1201 agcgtcgcgc cggtgcggaa taccgccgca tcgagctgca tggtcttctg catctccatg
     1261 cggatcgcgg cagtgccgcg cgcgccgttc gcgttgcgca gccggtcgag ccgcgccacg
     1321 gcctgctcgc cggccccggc gggcagcggc gcgtgcgggc tcgcgggcac gatggtcgcg
     1381 gcgcagcgcc gggcggcggc gcgcccgaac accaccaggt cgagcagcga gttgctcccg
     1441 agacggttgg cgccgtgcac cgacacgcag gcggcctccc ccaccgccat cagccccggc
     1501 accacgcggt cgggatcgcc ctccttcggg cacacgacct cgccgtgatg gttgcagggg
     1561 ataccgccca tgttgtaatg caccgtgggc tgcaccggga tgggctcgcg cgtcacgtcg
     1621 acgccggcga agatgcgcgc cgtctcggcg atgcccggca ggcgctcgtg gatgaccccg
     1681 ggcccgaggt gctcgagatg cagctcgagg tgatcgtgct cgacgccgac gccgcggcct
     1741 tcgcggatct ccaccgtcat ggcgcggctc accacgtcgc gcgaggcgag atccttggcg
     1801 ttgggcgcat agcgctccat gaagcgctcg ccggcggagt tggtgaggta gccgccctcg
     1861 ccgcgcgcac cttcggtgat caggcagccg gcgccgtaga tcccggtggg atggaactgc
     1921 acgaactcca tgtcctgcag cgcgagaccg gcgcgcagca ccatcgcatt gccgtccccg
     1981 gtgcacgagt gcgccgaggt gcacgagaaa taggtgcgcc cgtagccgcc ggtcgcgagg
     2041 atggtcacgt gcgcgcggaa acgatgcagc gtgccgtccg cca
//
LOCUS       DATDMP010000209         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_32008, whole genome shotgun sequence.
ACCESSION   DATDMP010000209 DATDMP010000000
VERSION     DATDMP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32008"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /gene="glp"
                     /locus_tag="VI195_03550"
     CDS             <1..1226
                     /gene="glp"
                     /locus_tag="VI195_03550"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY6823487.1"
                     /translation="GYDPDALRVDKAVAAIRACLAPIAETEKVPIREALGRILAQQIV
                     PSIDVPGHDNSAMDGYAVRGADLAPAQEVLLQQVGSAFAGRGFEDEIAKGQCVRIMTG
                     AVMPKGADTVVVQEVCRVEGGRVAIPAGQKRGQNVRAAGEDLKAGVPVMQPGQLLRAA
                     EIGLIASLGIGEVEVKRRLRVAFFSTGDELTSIGAPLKAGQVYDSNRYTLHGMLSRLG
                     VEIIDLGVVRDDPALLEAAFRKAAASADAIITTGGVSVGEADFVRQLMAKLGEVLFWK
                     IAMRPGRPMAFGRIGNAILFGLPGNPVAVMVTFYQFVRNALQFMSGMTGAEIPLPSAV
                     SSQAIRKVPGRTEYQRGVLFDEGGVCKVRTTGQQGSGVLRSMSEANCFIVLEHERGSV
                     QPGEPVRVQPFEGLA"
     gene            1258..1788
                     /locus_tag="VI195_03555"
     CDS             1258..1788
                     /locus_tag="VI195_03555"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY6823488.1"
                     /translation="MNAHSPIQPARAPLAQAAKSFGRTLLKDGGIEPGLAAALRDVVG
                     RSRSWLRPEHRVGDPARYMRHLLYVDPEDEFVITAITWLPGQKSPVHGHYVWCAYGVA
                     EGELTEELFRAPEHLLEPLGTRTLRAGDLADLDLGGPLYHRVSNRSAKPLVTLHLYGV
                     ASGSITTGINRIYGEE"
     gene            1785..>2175
                     /locus_tag="VI195_03560"
     CDS             1785..>2175
                     /locus_tag="VI195_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY6823489.1"
                     /translation="MTAGSRRAVAAWLFVCAALTFAIVVVGGITRLTESGLSITEWQP
                     LVGAVPPLQQSDWETLFARYRETPQYRKVFSEIGLEGFKSIFWWEYTHRLLGRAIGLV
                     FLLPFLYFLARGKLPKPLAWKLAGLLVL"
BASE COUNT          342 a          738 c          746 g          349 t
ORIGIN      
        1 acggctacga tccggacgcg ctgcgcgtgg acaaggccgt ggcggcgatc cgggcctgcc
       61 tagcccccat cgcggaaact gaaaaggttc cgatcaggga agccctcggt cgaatattgg
      121 cgcagcagat cgtgccctcg atcgacgtgc ccggccacga caactcggcg atggatggct
      181 atgcggtccg cggagcggat ctcgcgccgg ctcaagaggt tctcttgcag caagtcggat
      241 cggcattcgc cggaagaggt tttgaagatg aaatagcgaa gggccaatgc gtccggatca
      301 tgaccggcgc agtgatgccg aagggcgcgg atacggtggt ggtgcaggaa gtctgccgcg
      361 tggaaggcgg gcgcgtcgcg attccggcag ggcagaagcg cggccagaac gtgcgcgccg
      421 cgggcgagga tttgaaggcg ggcgtgccgg tgatgcagcc cggccagctc ctgcgcgccg
      481 ccgagatcgg cctcatcgcc tcgctcggca tcggcgaagt cgaggtgaag cgccgtctgc
      541 gcgtcgcctt cttctccacc ggcgacgagc ttacctcgat cggcgcgccg ctcaaggcag
      601 gccaggtcta cgattccaac cgctacacgc tgcacggcat gctctcgcgg ctcggcgtcg
      661 agatcatcga cctgggcgtg gtgcgcgacg acccggcgct gctggaggcc gcgttccgca
      721 aggccgccgc gagcgccgat gccatcatca ccacgggcgg cgtctcggtc ggtgaggccg
      781 atttcgtgcg gcagctcatg gcgaagctgg gcgaagttct gttctggaag atcgccatgc
      841 gccccggccg gccgatggcc ttcggccgga tcggcaacgc catcctcttc gggctgcccg
      901 gcaatccggt cgcggtgatg gtgacgttct atcagttcgt caggaatgcc ctgcagttca
      961 tgtcgggaat gaccggggcg gaaattccgc tgccgtccgc cgtcagctcg caggcgatcc
     1021 gcaaagtgcc ggggcgcacg gaataccagc gcggcgtcct gttcgacgag ggtggcgtct
     1081 gcaaggtgcg caccaccggc cagcagggct cgggcgtgct gcgctcgatg tccgaggcga
     1141 actgcttcat cgtgctcgag cacgagcgcg gcagcgtgca accgggcgag ccggtgcggg
     1201 tgcaaccgtt tgaaggactc gcgtgaaaca ccgtacgctc gtgcaggcag gcctggcgtg
     1261 aacgcacatt cgccgatcca gcccgcgcgc gcgccgctcg cgcaggccgc caagtcgttc
     1321 ggccgcacgc tgctcaagga cggcggcatc gagccgggcc tcgccgcggc gctgcgcgac
     1381 gtcgtcggca ggagccgcag ctggttgcgc cccgagcacc gcgtaggcga cccggcgcgc
     1441 tacatgcgtc acttgctcta tgtggacccc gaggacgagt tcgtgatcac cgcgatcacc
     1501 tggctgcccg ggcagaaaag cccggtgcac ggccactacg tctggtgcgc ctacggcgtc
     1561 gccgaaggcg agctcaccga ggagctgttc cgcgcgcccg aacacctgct cgagccgctc
     1621 ggcacccgga ccctgcgcgc cggcgacctg gccgacctcg acctcggcgg gccgctctat
     1681 caccgcgtct ccaaccgctc ggcgaagccg ctcgtgaccc tgcacctcta cggcgtcgcc
     1741 tccggcagca tcaccaccgg catcaaccgc atctacggcg aagaatgacg gccgggagca
     1801 ggagggccgt cgcggcctgg ctgttcgtct gcgccgccct caccttcgcg atcgtggtgg
     1861 tcggcggcat cacgcgcctg accgagtcgg gcctgtccat caccgagtgg cagccgctcg
     1921 tgggcgccgt gccgccgctg cagcagtccg actgggaaac gctcttcgcc cgctaccgcg
     1981 agaccccgca gtaccggaag gtgttctcgg aaatcggcct ggagggcttc aagagcatct
     2041 tctggtggga atacacgcac cgcctgctcg gccgggccat cggcttggtg ttcctgctcc
     2101 ccttcctcta ttttcttgca agaggaaagc tgccgaagcc actcgcctgg aaactcgccg
     2161 ggctgctggt gctgg
//
LOCUS       DATDMP010000210         2018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1142730, whole genome shotgun sequence.
ACCESSION   DATDMP010000210 DATDMP010000000
VERSION     DATDMP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142730"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VI195_03565"
     CDS             <1..901
                     /locus_tag="VI195_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6823490.1"
                     /translation="ETKLLSGHSNYDVVVPSDRFFDRQRAAGVYRELDKRALPNLANI
                     DPEIMRRMAAHDPGNLHAIPYMWSTTGLGYNIDLVRERLGGNVPDSWALLFDPRIAAK
                     LKDCGILIVDSPIDVFGSAIIYLQRDPRRRDPRDVLAAAQVLQAIRPFVRNIDPAPIA
                     PLASGDVCLELAWSGDVEAARNRAIEASTGARIAYLIPREGALTSVDMVAIPADAPHP
                     HNAALWLNYLMRPDVIAGISNYIKYPNGIAASLPLIDAAVRNDPAVYPDAATRARLTA
                     SVPVPLNYSRQVARAWTRFRTGQ"
     gene            complement(926..1339)
                     /locus_tag="VI195_03570"
     CDS             complement(926..1339)
                     /locus_tag="VI195_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6823491.1"
                     /translation="MSNGKSFLRSACAIANSLDIVGDKWSLLVVRDLLHGKGTYGELA
                     NSLERIPTNILADRLKRLEAAGIIVSTPYQQRPVRYRYSLTPKGNALGEVLLAFVRWG
                     KQHIPGTVALKRAATHAGAAAAPPSMRRGGARGGK"
     gene            1424..>2018
                     /locus_tag="VI195_03575"
     CDS             1424..>2018
                     /locus_tag="VI195_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823492.1"
                     /translation="MQVTEESTRSTTVLPPAAGSERSALAGIRRSLALVLGIWFLLVV
                     SLGGAGAFSAAPGSPPVAIALGVTVPLLLFFAWLRLSRSFRDFVLSLDLRLIAGVQAW
                     RWAGFGFLALYAHNILPGMFALPAGLGDMAIGVTAPWVMLALVRQPAFAASATFVRWN
                     VLGIIDLVVAVSLGALSATLTTGAPGEVSSAPMATLPL"
BASE COUNT          310 a          695 c          635 g          378 t
ORIGIN      
        1 cgagactaag ttgctgagtg gtcactcgaa ttacgacgtg gtggtcccca gcgatcgttt
       61 cttcgatcgt cagcgcgcag ccggcgtcta ccgcgagctc gacaagcgag ctttgcccaa
      121 tctcgccaac atcgaccccg aaatcatgcg ccgcatggcg gcgcacgatc cgggcaatct
      181 gcacgcgatt ccctacatgt ggtcgacgac cggcctcggc tacaacatcg acctggtgcg
      241 cgagcggctc ggtggcaacg tgcccgatag ctgggcgctg ctcttcgatc cgcgcatcgc
      301 cgcgaagctc aaggattgcg gcatcctcat cgtcgactcg ccgatcgacg tgttcggctc
      361 cgcgatcatc tatctgcagc gcgacccgcg ccggcgcgat ccccgggacg tgcttgccgc
      421 ggcacaggtg ctgcaggcga tacggccgtt cgtgcgcaat atcgacccgg cccccatcgc
      481 gccgctcgcg agcggcgatg tgtgtctcga gctcgcctgg tcgggcgatg tggaagccgc
      541 acgcaaccgc gcgatcgagg cttcgaccgg tgcacgcatt gcttacctga tcccgcgcga
      601 aggagcgctg acgtcggtag acatggtggc gatccccgcc gatgcaccgc atccgcataa
      661 cgccgcgctc tggttgaatt acctcatgcg ccccgatgtc atcgccggga taagcaacta
      721 catcaagtat ccaaacggta ttgccgcgtc gctgccgctc atcgacgccg ctgtcaggaa
      781 cgatccggcc gtgtatccgg acgccgccac gcgcgcacgg ctgacagcca gcgtgccggt
      841 gccgctcaac tactcgcggc aggtagcccg cgcctggacg cgctttcgca cggggcagtg
      901 atcgcccagc ctgcgctgcg cgtactcact tcccgcccct cgcaccccca cggcgcatgc
      961 tcggcggcgc tgccgcagcg ccggcgtgcg tcgcagctcg cttcagcgcc accgtaccgg
     1021 ggatgtgttg tttaccccag cgcacgaacg cgagcaacac ctcgccgagc gcgttgccct
     1081 tgggcgtgag gctgtagcgg tagcgcaccg gacgctgctg ataaggggtg ctgacgatga
     1141 tgccggcggc ttccagacgc ttgagacggt cggcaaggat gttggtcgga atacgctcga
     1201 gggaattcgc cagctcgccg taggtgccct tgccatgcag caggtcgcgc accacgagca
     1261 gcgaccactt atcgccgacg atgtcgagcg aattggcgat tgcgcaggcg gaacgcagga
     1321 aagacttgcc gttactcatt tgctccactg cctccgggcg gcaggccgcc cagtgacttg
     1381 catagtgcaa gtaataatgc tacggtgccc acaaacttgc aaaatgcaag tcactgagga
     1441 gagcaccagg agcaccaccg tccttccccc cgcggccggg tctgagcgca gcgcgctcgc
     1501 cgggattcgc cgcagcctcg cgctcgttct gggtatctgg ttcctgctcg tcgtgtcact
     1561 cggcggcgcc ggcgccttca gcgccgctcc gggcagcccc ccggtcgcga ttgcgctggg
     1621 ggtgacggtg ccgctgctgc tcttctttgc atggctgcgg ctctcgcgat cattccgcga
     1681 cttcgtgctc tcgctcgatt tgcgtctcat agcgggtgtg caggcgtggc gctgggcagg
     1741 cttcggtttc ctcgctctat atgcgcataa catcctgccc ggcatgtttg ccctgccggc
     1801 gggcctcggc gacatggcca tcggggtcac tgccccgtgg gtcatgcttg ccctcgtgcg
     1861 tcagcccgcc ttcgccgcga gcgccacttt cgtccgttgg aatgtgctcg gcatcatcga
     1921 cctggtcgtg gccgtcagcc tcggagcctt gagtgccacg ctgaccaccg gggcgccggg
     1981 tgaggtcagt agcgctccga tggcgacact gccgctgc
//
LOCUS       DATDMP010000211         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_921538, whole genome shotgun sequence.
ACCESSION   DATDMP010000211 DATDMP010000000
VERSION     DATDMP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921538"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..481)
                     /locus_tag="VI195_03580"
     CDS             complement(245..481)
                     /locus_tag="VI195_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823493.1"
                     /translation="MYLGRRLARGEQSSALRAGRWSNLRALGPYAVIALTVPGGSLIA
                     LAMLGIRHRAALPGLRGLVLVLIVATSIALTGST"
     gene            715..1644
                     /locus_tag="VI195_03585"
     CDS             715..1644
                     /locus_tag="VI195_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEY6823494.1"
                     /translation="MRALIVGAGSVGGYFGGRLAAAKRDVSFLVRPHRASQLARGLTL
                     SSQGQDITIPVRLLLSGETAGEFDVILLAVKAYQLERAIDDLAPYMSEHTMILPVLNG
                     MQHMDVLRSRFGARRVIGGVARIAVSLDEQGRIVDQANFHDLAYGEWSGERSSRIVAL
                     DEYMRAARFDARLSTEIEREMWEKWAVLASLGAITCLMDGDIGRVARTPGGTEFVRAL
                     FAEVLATIGAAWRALGEPFKSRALALLSDKSSTLTSSMYRDMKAGQRIEADQIIGDLV
                     RRAAALGVATPLLSTVLLRLQAYEQLQGLERER"
     gene            complement(1663..>2306)
                     /locus_tag="VI195_03590"
     CDS             complement(1663..>2306)
                     /locus_tag="VI195_03590"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY6823495.1"
                     /translation="SGHWEERDFAVMASPFPPMVYDYSGFPEYLYHIRYGAPGAPELA
                     RRAHSLIQATGLATHLDPRRGFDHGTYSLLAVVYPEAAVPVMQVSLQTGYDPAAHLQL
                     GRALAPLRDEGILIIGSGMSYHNFHPIHPREDSAQFDFWLRQTLVDSAPQQRSQRLLE
                     WEHAPAGRAAHPREDHLIPLHVAAGAAEEAPARIVYRQEDYLGRITLSSYRFG"
BASE COUNT          414 a          718 c          795 g          379 t
ORIGIN      
        1 acaccggcag aggcggcggc gcgatggtga ccgagaactc gacttgtgca gccgcagtcg
       61 tcggtaacgc caccgcgcca agcgttaccc acacggccag cagcaacgcg gcaacgggtg
      121 tcttgatcat tgcgctacgc ctcagtggat tgcgagcgtg caactgtgcg cgccggtcgc
      181 agacgatgca atatcggaga tgaatcgtcc gtgaacgcgt tgacgtcgca gcgtagcgtc
      241 gctatcaggt gctgccggta agggcgatcg aggtggcaac gatcaggacg aggaccagtc
      301 cgcgcagtcc cggaagtgcg gcgcggtgcc tgatacccaa catcgcgagg gcgatcaggc
      361 ttccgccagg aacagtcagt gcgatgacgg catagggacc gagtgcacgc aggttcgacc
      421 aacggcccgc gcgaagcgca gaactctgtt caccacgggc gagccgccgg ccgagataca
      481 tgaagatgac tccgtggacc cacagttaag gatccttgtg gccggcattc tgggaccggc
      541 gcgcagcgac tcccatcccg caagttggcg gccgagcctc gtcccctacg gcgtgtgagt
      601 gcctatctct aaagtggggg acgcggtcgt gtatgcggtg gcagctgggt cgcagcgcag
      661 gttcttcgag tacgatctgc tagcgaggcg cgcagcgcgc aacggaggcc acgcatgaga
      721 gcgctgatcg tcggagccgg tagcgtcggt ggctacttcg gcggccggct ggcggctgcc
      781 aaacgagatg tgagcttctt ggtccggccc caccgggcca gccagctcgc ccgtgggctc
      841 accctctcga gccaggggca ggacatcacc attccggtga ggttgctgct cagcggcgag
      901 acagccggtg agttcgatgt gatcctgctc gccgtcaagg cctatcagct cgagcgcgcg
      961 atcgatgacc tcgcgcccta catgagcgag cacaccatga ttctgccggt gctgaacggc
     1021 atgcagcaca tggatgtgct gcgctcccgg tttggagcgc gccgggtcat cggcggtgtc
     1081 gcgcgcatcg ccgtgagcct cgatgagcag ggccgcatcg tcgatcaggc gaatttccac
     1141 gatctcgcct acggcgagtg gagcggagag aggtcgagcc gcatcgttgc gctcgatgag
     1201 tacatgcgcg cagcgcgatt cgatgcgcgg ctctccacag agatcgagcg cgagatgtgg
     1261 gagaagtggg cggtgctcgc gagcctcggt gccatcacct gtctgatgga tggcgacatc
     1321 gggcgcgtcg cccgcacgcc gggcggtacc gagttcgtgc gggcactgtt cgccgaggtc
     1381 ctggccacga ttggtgcggc ctggcgggcg ctcggagagc ctttcaaatc ccgggccctc
     1441 gcgctgctca gcgacaagag ctcgactctc acctcatcca tgtaccggga catgaaggcg
     1501 ggccagcgca tcgaggcgga tcagatcatc ggcgatctgg tgcggcgcgc ggctgcgctc
     1561 ggggtcgcga cgccactcct gtccacggtg ctgttgagac tgcaagcgta tgagcagctg
     1621 caaggcctcg aacgcgagcg ctgattcccg catcgcccac ggtcagccaa aacggtaact
     1681 cgatagcgtg atccggccga ggtaatcctc ctgccggtac acgatccgcg ccggcgcttc
     1741 ctcggccgct ccagccgcta cgtgcagcgg aatgaggtga tcttcccgcg ggtgcgcagc
     1801 ccgcccggca ggagcgtgtt cccactcgag cagccgctgc gagcgctgct gcggagcgga
     1861 gtcgaccagt gtctggcgca gccagaaatc gaactgtgcc gagtcctcgc gcggatggat
     1921 cggatggaaa ttgtggtagc tcatgccgct gccaatgatg aggatgccct catcgcgcag
     1981 cggcgcgagt gcgcgcccga gctgcagatg cgccgccggg tcatagcccg tttgcagcga
     2041 gacctgcatg accgggaccg cagcctccgg gtagaccacg gccagcagcg aataggtgcc
     2101 atgatcgaac ccgcgccgcg gatcgagatg agtcgcaagc ccggtcgcct gaatgagcga
     2161 gtgcgcgcgg cgtgcgagct ctggcgcacc cggggcgcca taacggatgt gatagagata
     2221 ctcggggaat ccgctgtagt cgtagaccat gggagggaag gggctggcca tcacggcgaa
     2281 gtccctttcc tcccaatggc ccgaga
//
LOCUS       DATDMP010000212         3560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2143940, whole genome shotgun sequence.
ACCESSION   DATDMP010000212 DATDMP010000000
VERSION     DATDMP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143940"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..311)
                     /locus_tag="VI195_03595"
     CDS             complement(87..311)
                     /locus_tag="VI195_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823496.1"
                     /translation="MTEANQQAIRAFFAGLDEEAHDLAERLAHGDDESIWAWFERRYP
                     KATKRAAKDRHWRDFVRAIREFYRDDSALQ"
     gene            542..793
                     /locus_tag="VI195_03600"
     CDS             542..793
                     /locus_tag="VI195_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823497.1"
                     /translation="MATMLDKTLKREVLIGDRSYILAISPLTLKLTPKGKRKGLELHW
                     ESLVSGDAALAAALNASVGKFDPGRGRLKPGDQSGGSTD"
     gene            867..1742
                     /locus_tag="VI195_03605"
     CDS             867..1742
                     /locus_tag="VI195_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6823498.1"
                     /translation="MPRRSWRELRQTLNNWLDHDGPRLAASLSLYSLLSLAPLVILSI
                     AIASLAFGRAAAQSAVLQEVRGVMGADGARTIQTVIEHGKSPQAGSAASAIGIVILLF
                     GASSVFSELQAGLNKVWDAHTPTGYGAWALVKSRLVSFALVLSFGFLLLVSLLFSAAL
                     AAFGHYLSDHLSLPRALLAALNVLISLAGSFVLIGLILRYVPDVSLRWRDVWQGALAT
                     ALLFSVGKALLGLYLGKAAVGSAYGAAGSLIVVILWVYYFAMIFYFGAEFTRVRARRA
                     SVSSPQAAAITEGRR"
     gene            1739..2083
                     /locus_tag="VI195_03610"
     CDS             1739..2083
                     /locus_tag="VI195_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6823499.1"
                     /translation="MKHAQLRFGKGFHVVFGNRRAQVAEMVIEPGGSEGDPSNRHHGA
                     DQWLYVVSGAGLASVNGKRVPLRAGVLVLIERNDRHEIRNTGRGRLRTLNYYTPPAYT
                     KAGEDLPAARRS"
     gene            complement(2158..>3560)
                     /gene="ligD"
                     /locus_tag="VI195_03615"
     CDS             complement(2158..>3560)
                     /gene="ligD"
                     /locus_tag="VI195_03615"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY6823500.1"
                     /translation="ARSAEAMLHNGSLRFKLDGERLHGGWVLVRMRNDRTNGRRRNWL
                     LIKHHDAFERAGNGNGASAEDQSVASGRTMAEIAAGKGRRPRPFMLKGARYRSDAVWN
                     SNRSEASDAASAAESLPHAKRTEVKVEKPARATTPTLSAPLRRLATARGSGRRRSRAD
                     TSAPVVMGVTISNPQKSLWPATKGNEAISKLELARYYEAVGAWMMEHIRGRPCSIIRA
                     PDGIGGERFFQRHAMLGASSLIELVKISGDRKPYLQIDRVEALAALAQVAALELHPWN
                     CAPGNPERPGRFVFDLDPAPDVAFDRVVEAASELRTRLEKLGLVAFCKTTGGKGLHVV
                     TPFSLPRSPKIGWPEAKALAREICVRMAADSPRKYLLTMAKKERSGRIFLDYLRNDRM
                     ATAVAPLSPRARAGAPVSMPLNWAQVKKGLDPMRYTLRSVPALLARSTAWKEYCQCER
                     PLLAALKRASSGAKAV"
BASE COUNT          600 a         1127 c         1156 g          677 t
ORIGIN      
        1 tgatagcgcg cacgctcaag cgcgcgtagg agaagtccga agctcaccgc gctgggggat
       61 tgccgcagac gcggcaggta gcgctgctac tgcagggcgc tgtcgtctcg gtagaactcg
      121 cggatcgccc gcacgaaatc acgccaatgc cggtctttgg cagcacgctt ggtggccttc
      181 gggtagcgtc tttcgaacca cgcccagatg ctctcatcgt caccgtgggc caaacgctcg
      241 gcgagatcgt gggcctcctc atcgaggccc gcgaagaaag cgcgtatcgc ctgttggtta
      301 gcttcggtca cgaaaacaac gatggcccgc gttacgtcaa caagcaagcg tggccgtggt
      361 gatcaacagc gtgcagcggc agcactgcgg ccgaactcgt tgacggggtg ctccaccgca
      421 caggcgtgac acacgctgcc ctcgaggcct agctcacggc ggttccgcac tcgttcgccc
      481 ggcgtctgta tatataagta ggccactgca aacgaacgcc gcaacgggcg gtctcacctg
      541 catggccacc atgctggata agacgctcaa acgcgaagtg ctcatcgggg accgctccta
      601 catcctggcg atctcgccgc tcacactgaa gctgacgccg aagggcaagc gcaagggtct
      661 ggagctgcac tgggagtcgc tcgtgagcgg cgatgctgca ctcgccgcgg cgctcaacgc
      721 ctcggtgggc aaattcgacc cgggccgtgg gcgtctgaag ccgggcgacc agtcgggtgg
      781 atcaacggac tgacagtcga cgcgcttgcc ggacgcagag gccgagagct cttccggccg
      841 ccggtgtcgg ccgacagatg tgggcgatgc cgcgtcgatc ctggcgggag ctcaggcaga
      901 ctctcaacaa ctggctggac catgacggtc cgcgcctcgc cgcatcgctc tccctgtaca
      961 gcctgttgtc gctcgcccca ctcgtcatct tgtccattgc gattgcctcg ctcgcgttcg
     1021 ggcgcgccgc ggcacagagc gcggtcctcc aggaggtccg cggtgtgatg ggagcagatg
     1081 gagcacgcac cattcagacc gtgatcgagc atgggaaatc gccccaagca gggagcgctg
     1141 cttcggcaat cggaatcgta atcctgctgt tcggtgcttc gagtgtgttc agtgagttgc
     1201 aagcaggtct caataaggtg tgggatgcgc acacgccgac gggatacggg gcctgggcac
     1261 tggtcaagtc tcgactggtg tcctttgcgc tggtactcag tttcggcttt ctgctgctgg
     1321 tctcgctgct gttcagcgca gcgctggccg ccttcggcca ctacctcagt gaccacctct
     1381 ccttgccgcg ggcgctgttg gctgccctga atgtgctcat ctcgcttgcg ggcagtttcg
     1441 tgctgatcgg gctcattctc agatacgtac ccgatgtgag cctgcgttgg agagacgtct
     1501 ggcagggggc tctcgcgacc gccttgcttt tctcggtcgg caaggcgctg ctcggactct
     1561 acttgggaaa ggcggccgtg ggctcagcct acggcgctgc gggctcgctc atcgtggtga
     1621 tcttgtgggt ctactatttc gccatgattt tctacttcgg ggcggaattt actcgtgtcc
     1681 gcgcccggcg cgcgagtgtt agctcgcctc aggctgcagc catcacggag ggtcgccggt
     1741 gaaacacgct caactgcgct ttggcaaggg atttcacgtg gtctttggaa atcgccgcgc
     1801 acaggttgcc gagatggtga ttgagcccgg cgggagcgaa ggtgacccga gcaaccggca
     1861 ccacggggcc gatcagtggc tgtatgtcgt gagcggggcc ggtttggcat cggtgaatgg
     1921 caagagggtt ccgctgcggg ccggggtttt ggtactcatc gagcgaaacg atcgccacga
     1981 gatcaggaat accggccgtg ggcgcctgcg tacactcaat tactacacac ctccggcgta
     2041 cacaaaagcc ggtgaggacc tgccagcggc acggcgcagt tgattagcgt gttgctcgca
     2101 cactgacctg gcgcgtcgcg gcgagcgtgc tcgcgaacaa ctcggccgga gaccgaatca
     2161 cactgccttg gcgccggagc tggcacgttt gagggccgcg agcaacggcc gctcacactg
     2221 gcagtactcc ttccaggcag tcgatctcgc cagcagcgcg ggcacgctgc gcaaggtgta
     2281 acgcatcggg tcgagcccct tcttcacctg cgcccaattg aggggcatcg aaacaggcgc
     2341 tcccgcacgg gcgcgcggcg agagcggcgc cactgcggta gccatgcggt cattgcgcag
     2401 ataatcgaga aagatgcgac cgctgcgctc tttcttggcc atagtcagca aatatttcct
     2461 agggctgtcc gcagccatgc gcacgcagat ctctcgggcc agagccttgg cctcgggcca
     2521 gccaattttg gggctgcggg gtagactgaa gggagtcacc acgtgcaagc ccttgccacc
     2581 ggttgtcttg cagaacgcca ccaggccgag cttttcgagc cgcgtgcgca gctcgctcgc
     2641 ggcttcgacg acgcgatcga acgcgacatc cggcgccgga tcgagatcga agacgaagcg
     2701 ccccggccgt tccgggtttc cgggtgcgca gttccagggg tgcaattcca gcgccgccac
     2761 ctgggcgagc gcggccaggg cctcgacgcg atcgatctga agatagggct tacggtcgcc
     2821 ggagatcttc accagctcta tgagactcga ggcgccgagc atcgcatggc gctgaaagaa
     2881 ccgctcgccg ccaattccat cgggagcacg gatgatcgag cagggccggc cgcggatgtg
     2941 ctccatcatc cacgctccca ccgcttcgta gtagcgcgcg agctccagct tgctgatggc
     3001 ttcattgcct ttcgtggccg gccagagtga cttttgtgga ttcgagatcg tgactcccat
     3061 gaccaccggt gcggaagtgt cagccctgct gcgcctccgg ccagagcctc gggccgtcgc
     3121 gagacggcga agcggcgcac tgagagtggg cgtcgttgct cgcgccggtt tctccacctt
     3181 tacctcggtg cgcttggcgt gcggcaacga ctcggctgcg gacgcggcgt cgctcgcctc
     3241 gctgcgattg gaattccaca cggcgtccga gcggtaccga gcacccttca gcatgaaggg
     3301 gcgtggtctg cgccctttgc ccgccgcgat ctcggccatg gtgcgcccgg acgccaccga
     3361 ctggtcctcc gctgaagcgc cattgccatt gcctgcgcgc tcgaaagcat cgtggtgctt
     3421 gatgagaagc cagtttcgcc ggcgtccgtt cgtgcggtcg ttgcgcatgc gcaccaggac
     3481 ccacccgccg tgcagacgct caccgtcgag tttgaacctg agactcccgt tgtggagcat
     3541 cgcttctgca gagcgtgccc
//
LOCUS       DATDMP010000213         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1255122, whole genome shotgun sequence.
ACCESSION   DATDMP010000213 DATDMP010000000
VERSION     DATDMP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255122"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VI195_03620"
     CDS             <1..816
                     /locus_tag="VI195_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6823501.1"
                     /translation="ELPDGTTVAALGQGCWHLGQGRHPLPVEEEALRTGISLGMTLID
                     TSGNYGHGRSEEFISHVIAGQRDRIFLVSKVEGNEVSGDGIARACAASLARLGTDHLD
                     LYLLHWPVSSRQFAGVVAGFEQLRAAGKIRAWGVSNFDAEQMEDLFRVPDGHRCATNQ
                     VAYSLNNRRIERDLLPWCQQHNVPVMAYSPLGGDNHLLVGDRTLAQIGATHECSAAAV
                     ALAWVIRGGNVIAIPESGSPAHVRENAVALSMALAPQDLRTLDAAYPGPFGAG"
     gene            complement(848..1033)
                     /locus_tag="VI195_03625"
     CDS             complement(848..1033)
                     /locus_tag="VI195_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823502.1"
                     /translation="MSHALPADLPVPLLERLRAEGIATLEAWVALGRRRRQIFGVTRA
                     AVLQLDALAKAALKPAP"
     gene            1426..1731
                     /locus_tag="VI195_03630"
     CDS             1426..1731
                     /locus_tag="VI195_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823503.1"
                     /translation="MHLGPDNALKSAELLGGGSFLPVHWGTFSLAMHAWDQPPERLLE
                     LAPRSAARIMMPRLGEAVEPAHASGRIDPWWRSVDSAEQPLSPSAGTLPATVPWPID"
     gene            1798..>2246
                     /locus_tag="VI195_03635"
     CDS             1798..>2246
                     /locus_tag="VI195_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6823504.1"
                     /translation="MSTVTDYFFSSGDGLELYCAVYPARQPPGLPVLCLPGVTRNSKD
                     FAELAGRICEHHEVLAADLRGRGRSAWDPQASHYQIGTYIQDVLTLLASRGIRRVVVI
                     GTSLGALLGMTLAAMRPDLIAGVVLNDAGPQIDPAGVRRIAGYIGKLP"
BASE COUNT          358 a          740 c          764 g          384 t
ORIGIN      
        1 gagcttccgg atggcaccac cgttgcggcg ctcggtcagg gctgctggca tctcggacag
       61 gggaggcatc cgctgcctgt cgaggaagag gcgctgcgca ccggcatttc cctcggcatg
      121 acgttgatcg atacgtcagg aaattacggc cacggccgct ccgaggaatt catcagtcac
      181 gtgattgccg gtcagcgcga ccgtatattc ctggtctcca aggtggaggg caatgaagtg
      241 tccggagatg gcatcgcgcg tgcgtgcgcg gcgagtctcg cgcgtctcgg caccgaccat
      301 ctcgatctct acctgctgca ttggccggtt tccagcaggc aattcgccgg cgtcgtagcc
      361 ggattcgaac aactacgcgc ggcgggaaag attcgcgcct ggggcgtatc caacttcgac
      421 gctgagcaga tggaagatct gttccgtgtc ccggacggtc atcgctgcgc aaccaaccag
      481 gtcgcctaca gtctcaacaa ccgtcgcatc gagcgtgacc ttctgccatg gtgccagcag
      541 cacaatgtgc cggtgatggc ctactcgccg ctcgggggcg acaatcacct cctggtcggt
      601 gaccgcacgc tggcgcagat cggcgccacg catgaatgct cggccgccgc ggtcgcgctg
      661 gcctgggtca ttcgcggcgg caacgtcatc gccattcccg aatccggctc cccggcgcat
      721 gtcagggaaa acgccgtcgc gctctcgatg gcactcgccc cgcaggacct gcggacattg
      781 gacgcagcgt atccgggacc gtttggcgcc gggtagtacg agtcgcctgc cgggcgggaa
      841 ccgggcgcta cggcgcgggt ttcaacgccg ctttcgccag cgcgtcgagt tgcaggactg
      901 cggcgcgtgt gacgccgaag atctgccgac ggcgccgccc gagggcgacc caggcctcga
      961 gcgttgctat gccctcggcg cggaggcgct ccagcagcgg gaccggcaga tcggctggca
     1021 atgcgtgtga catgcggcca gtcttcgcag cccggctcat ttgccaagtg caatttaaca
     1081 ttaatgtccc aatgccttcc cgttcagtga cgaggttcgc gcagcagcgc cggctgcaac
     1141 cgttcgggga gcgtccagcg cgggtgtatg agccgaggca tcggcgctcg gcgtgccgct
     1201 cgggcggctc cttcaggtgg acaacccggc gttccgccgc tttactcatc aaccgggctc
     1261 gcatccgggt tcaagctgct cacctgacgg ccaggctgtg aaatcgctcg gatcaagagc
     1321 gagcggtgcc cgcctggagc gcatgcgagc atcgccgcgc tgggcgggcg agggcttcag
     1381 gaatctgcgc tcgaggtcgg cggctggaat gcggcctggg gcgatatgca tctggggccg
     1441 gacaacgccc tcaagtcggc cgaacttctg ggcggcggca gctttctgcc ggtgcactgg
     1501 gggaccttca gtctggcgat gcacgcgtgg gaccagccgc ccgaacggct gctcgagctg
     1561 gctccgcgga gtgccgcgcg catcatgatg ccgagactcg gagaggccgt ggagcccgcg
     1621 cacgcctccg ggcgaatcga tccctggtgg cgaagcgtgg acagcgcgga acagccactc
     1681 agcccgagcg cgggcacgct cccggcgacc gtgccctggc cgatcgatta gctgggtcag
     1741 ggacgagaga gcttcccccg cgtcgtcgac ccgcaaccag cacgggctga tcgcatcgtg
     1801 agcacggtga ccgattactt cttcagcagc ggcgacggtc tggagctcta ttgcgcggtg
     1861 tatcccgcac ggcaaccgcc aggactgccg gtgctgtgtt taccgggcgt gacgcgcaac
     1921 agcaaggact tcgccgagct cgcggggcgc atctgcgagc atcacgaggt gctggcggcg
     1981 gatctgcgcg ggcgcgggcg ctctgcgtgg gacccgcaag cctcgcacta tcagatcggt
     2041 acctacattc aggacgtgct gacactgctc gcatcgcgcg gcatccgtcg cgtcgtggtg
     2101 atcggcacgt cgctcggcgc actgctgggc atgacgctcg ccgccatgcg cccggatctc
     2161 atcgccggcg tcgtgctcaa cgatgcgggt ccgcagatcg accccgcggg cgtgcgtcgc
     2221 atcgccggct acatcggcaa gctgcc
//
LOCUS       DATDMP010000214         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_810763, whole genome shotgun sequence.
ACCESSION   DATDMP010000214 DATDMP010000000
VERSION     DATDMP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810763"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VI195_03640"
     CDS             complement(<1..1069)
                     /locus_tag="VI195_03640"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEY6823505.1"
                     /translation="MAGKLEDVGTTIFTIMSRRARELGALNLGQGFPDYDIDPRLTQL
                     VAAAMQAGHNQYAPMEGLPALRERIAAKLLASYGRAIDPDTEITVTLGATEAIYSAVQ
                     ALIGAGDEAIAFDPAYDSYEPAVRLAGGRCLRVPLSQPGFRYDWERVRRALSSRTRLV
                     LLNSPHNPACTIARREDLDELARIVEGRAILVLSDEVYEHVVFDGAQHASVLAHPQLA
                     ARSLAVFSFGKTLHATGLRVGYCVAPAELTRELRKVHQFNTFSISHPLQHAIAAYLAA
                     MPGCGEQLAAFFQAKRDRLRSALEDSGFVLPKAEGTYFQLLDFHAFAPGPADDRAFAE
                     RLLNEAHVATIPLSPFYAQPEP"
     gene            complement(1080..1826)
                     /locus_tag="VI195_03645"
     CDS             complement(1080..1826)
                     /locus_tag="VI195_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY6823506.1"
                     /translation="MAGEVLGIDVGGSSVKAGLVDVQHGSVRGSLISAPTPRPCTPAS
                     LIPVLVALAGRLANAAGRVGVAFPSVIKGGTAFTAANIDHSWIGTNGESLAAEALGRP
                     VLMLNDADAAGIAEMRFGSGRGCSGTVIMLTFGTGIGTALFTGGRLFANTELGHLELR
                     GMDAEKWASAHVRTAAGLDFPGWIARVNEYLAALYQLLWPDVFILGGAVSEHFSEFAP
                     LLRSAAEIRPARFAGQAGVVGAALAAAAQD"
     gene            complement(1863..>2179)
                     /locus_tag="VI195_03650"
     CDS             complement(1863..>2179)
                     /locus_tag="VI195_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6823507.1"
                     /translation="RPVTVFGRITLPLILPAIASGWLLAFTLSWDDLVITQFVAGPGS
                     STLPMVIFSKVRFGVTPDVNALATIMVLIVATGIALSTVWMRYRERRRERDLQLAAAA
                     NL"
BASE COUNT          376 a          733 c          763 g          307 t
ORIGIN      
        1 gcggctcggg ctgcgcgtag aacggcgata gcgggatggt ggcgacgtgc gcctcgttga
       61 gcagccgttc ggcgaacgcg cggtcatccg ccgggcccgg ggcaaacgcg tggaaatcga
      121 gcagctggaa ataagtcccc tccgccttcg gcaacacgaa gcccgaatcc tcgagcgcac
      181 tccgcagccg gtcgcgcttg gcctggaaga acgccgcgag ctgctcgccg cagccgggca
      241 tcgcggcgag ataggccgcg atggcgtgct gcagagggtg ggagatgctg aaggtattga
      301 actggtgcac cttgcgcagc tcgcgcgtga gctcggcggg tgcgacgcaa tagccgacgc
      361 gcagcccggt ggcatgcaga gtcttgccaa aggagaacac tgcgagactg cgcgccgcca
      421 gctgcgggtg cgccagcacg gacgcgtgtt gcgcgccatc gaacaccacg tgctcgtaca
      481 cctcatcgga cagcacgagg atggcgcggc cctcgacgat gcgcgccagc tcgtccagat
      541 cctcgcgacg cgcaatggtg caggcgggat tgtgcggact gttcaacagc acgagacgcg
      601 tgcggctgct gagcgcgcgc cgcacacgct cccagtcata gcggaagccg ggctggctca
      661 gcggcacacg caggcagcgc ccgccggcga gccgcaccgc cggctcgtag gaatcgtagg
      721 cggggtcgaa ggcgattgcc tcatcgccgg cgccgatgag cgcctgcacc gcggagtaga
      781 tggcctcggt ggcaccgagg gtgacggtga tctcggtgtc cggatcgatc gcgcggccgt
      841 aggaagcgag cagcttggcg gcgatgcgct cgcgcagcgc gggcaaaccc tccatcggtg
      901 cgtactggtt gtgtccggcc tgcatggcgg cggccaccag ctgtgtgagc cgcgggtcga
      961 tgtcgtagtc gggaaagccc tggccgaggt tgagggcacc gagctcgcgc gcgcggcgcg
     1021 acatgatcgt gaagatcgtg gtgccgacgt cctcgagctt gcccgccacg gcggtgattt
     1081 cagtcctgcg cagcggcggc gagcgcggca cccaccacac ccgcctggcc ggcaaagcgc
     1141 gccggacgta tttccgcagc ggagcgcagc agcggggcaa attcactgaa gtgctcgctc
     1201 accgcgcccc cgagaatgaa cacatcgggc cacagcagct ggtacagcgc cgccaggtac
     1261 tcgttcacgc gtgcgatcca tcccggaaaa tcgagcccgg cggccgtgcg gacgtgcgcc
     1321 gaggcccatt tctccgcatc catgccgcgc agctcgagat ggccgagctc ggtgttggcg
     1381 aacagcctcc cgccggtgaa cagcgccgtg ccgataccgg taccgaaggt gagcatgatg
     1441 acggtgccgc tacaaccgcg gccgctgccg aagcgcatct ccgcgatgcc agcggcgtcg
     1501 gcgtcgttca gcatcagcac cggccggccg agggcctccg ccgccagcga ttccccattg
     1561 gtgccgatcc agctgtgatc gatattggcg gcggtgaagg cggtaccgcc cttgatgacg
     1621 ctcgggaagg cgaccccgac ccgccccgca gcgttggcga gccgcccggc gagggccacg
     1681 agcaccggta tcagggaagc gggcgtgcag ggacgcgggg tcggagcgga gatgagactg
     1741 ccgcgcacgc tgccgtgctg cacatcgacc agcccggctt tcacggatga tcccccgacg
     1801 tcgatgccca gtacttcgcc cgccatgtag ctactccccg cctgtccgac cctctccggc
     1861 gctcacaggt tcgctgccgc cgcgagctgc agatcgcgct cgcgccgccg ctcgcgatag
     1921 cgcatccaca ccgtcgagag tgcgatgccg gtcgccacga tcagcaccat gatggtggcg
     1981 agcgcgttga catcgggcgt cacgccgaag cggactttcg agaagatgac catcggtaac
     2041 gtgctcgacc cgggtccggc cacgaactgc gtgatcacca gatcatccca cgaaagcgtg
     2101 aaggccagca gccagccgga agcgattgcc ggcaggatga gcggcagcgt gatgcggccg
     2161 aacacggtca ccgggcgcg
//
LOCUS       DATDMP010000215         4222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_699767, whole genome shotgun sequence.
ACCESSION   DATDMP010000215 DATDMP010000000
VERSION     DATDMP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699767"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VI195_03655"
     CDS             <1..1405
                     /locus_tag="VI195_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823508.1"
                     /translation="VDGESRERFMTEIGFITESKNAKYQSWVVGKALRKTQAFASRIA
                     EITYVGKEAVFDTTQQDHNTVIFNGLVTGQCGEQPLPPYGSCLLGSVNLTRFVKHPFT
                     DFAEFDWHEYREVVKVFTRMLDNVVEVNGLPLEKQREEILRKRRHGMGFLGLGSTLTL
                     LRMKYGSPESVQFTEAVAREMAVAGWEAALELAREKGPAPIMNEEFTITREMLRKRPE
                     MARDGWRPGAKIAGRLLHAKYSRYMQRVAQVAPQLVHELAESGARFTHHSSIAPTGTI
                     SLSLANNASNGIEPSFAHHYFRNVIREGKKSKEKIDVYSFELLAYRELVNPSANPGAT
                     NDAERLPDYFIASDDVTPKEHVEVQAAAQKWVDSSISKTANVPTDFPYEKFKDIYLYA
                     HEQGLKGCTTFRFNPEAFQGVLVKEQDLKNTTYKFTLEDGTMVELRGDEEVEYDGELH
                     TAANLYDSLKEGYFGKF"
     gene            1429..2172
                     /locus_tag="VI195_03660"
     CDS             1429..2172
                     /locus_tag="VI195_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823509.1"
                     /translation="MTKIDGKIIKYAVQKPEDKSAADKPAEPKAPPPPASESEVVRDK
                     NGRTAKVIRMHEKLERPEMLVGSTYKIKTPISDHAMYVTINDIVLNEGTEYEQRRPFE
                     IFINSKNLDHFQWIVALTRIISAVFRKGGDVTFLVDELKAVFDPRGGYWQPGGKFMPS
                     IIAELGHVIEKHLQAIGLMRRRELDVHQQKLVDEKRAAFEARAKQTDAFAKTHFPEGA
                     QLCGRCSTAAVVMMDGCMTCLNCGDSKCG"
     gene            2868..>4222
                     /locus_tag="VI195_03665"
     CDS             2868..>4222
                     /locus_tag="VI195_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6823510.1"
                     /translation="MGLATWLLALAAALSCARALAAEDSMPRPPQLERDVQFWVRVYS
                     QIDTNAGFLHDQTNLGVVYDTLHFAPDAPPSERQRQVEQARERVAATLRRIAGSGEAP
                     LSAEDQRIRDLWGAEGTPARLRAAVDDIRFQLGQSDRFRSGLIRSGAWETHIAETLAN
                     LGLPAELAVLPHVESSFNPSAYSKVGAAGLWQFMRSTGRRYMRIDGAVDDRLDPFRST
                     EAAAQLLAYNYRVLGTWPLALTAYNHGTAGVRRAKETLGTDDIVRIVRSYSSRTFGFA
                     SRNFYVSFLAALEIDRNPEKYFGSLQKESEAHFQELELPAYVSATSLEHVLKLDAGKL
                     RALNPALLPAVWNGAQRVPKAYRLRLPADGSPWSSQLLAQRLTPGEMFTGQRAPPHYR
                     VQRGDTLAALASRYGVPIESLAELNHLRTSAHLRPGRVLTVPETTSISLAAASAAPAS
                     VP"
BASE COUNT          787 a         1365 c         1330 g          740 t
ORIGIN      
        1 tgtcgacggt gaatcgcgcg agcgtttcat gacggaaatc ggttttataa ccgaaagcaa
       61 gaacgcgaaa taccagagtt gggttgtcgg caaggcactc cgcaagactc aagccttcgc
      121 cagccgcatt gccgagatta cctatgttgg caaggaggcg gtgttcgata ccacgcagca
      181 agaccacaac accgtgatct tcaacggcct tgtgaccggg caatgtggtg agcagcctct
      241 tccgccctac ggctcgtgcc tgctcggctc ggtaaacctg acccgtttcg tcaagcatcc
      301 cttcaccgat ttcgccgaat tcgactggca cgagtaccgc gaggtcgtga aggtcttcac
      361 gcgcatgctc gacaacgtgg tcgaggtcaa tggcctgccg ctcgagaagc agcgcgagga
      421 gatcctgcgc aagcgccgcc acggcatggg gttcctcggg ctcggcagca ccctcacgct
      481 gctgcgcatg aaatacggct cgccggaatc ggtgcagttc accgaggcgg tcgcgcgcga
      541 gatggccgtg gcgggctggg aggctgcgct cgagctcgcc cgcgagaagg gcccggcgcc
      601 gatcatgaac gaggagttca ccattaccag ggagatgctg cgcaagcggc cggagatggc
      661 acgcgatggc tggagacccg gcgcgaagat cgccggccgg ctgttgcacg ccaaatacag
      721 ccgttacatg cagcgcgtcg cgcaggtggc gccgcagctg gtgcacgagc tggcggagag
      781 cggcgcgcgc ttcacgcacc acagctcgat tgcgccgacc ggcaccatct cgctctcgct
      841 tgccaacaac gcgtccaacg gcattgagcc gtcgttcgca catcactatt tccgcaacgt
      901 catccgggag ggcaagaagt caaaagagaa gatcgacgtc tattccttcg agttgctggc
      961 ctatcgcgag ctggtcaacc cgagcgcaaa ccctggagcc accaatgatg ccgagagact
     1021 gccggactat ttcatcgcgt ccgatgacgt cacgcccaaa gagcatgttg aagtgcaggc
     1081 tgcggcccag aagtgggtcg attcatcgat ttctaagaca gcgaacgtgc ctacggattt
     1141 cccctacgag aagttcaaag acatctatct gtatgcacac gagcagggcc tgaagggctg
     1201 caccacgttc cgcttcaatc cggaagcgtt ccagggcgtt ctggtcaagg agcaggacct
     1261 gaagaacacc acttacaagt tcacgctcga agacggaacg atggtcgagc tgcgcggtga
     1321 cgaagaggtc gaatacgacg gcgagctgca taccgcggcg aatctctacg acagcctcaa
     1381 ggagggctac ttcggaaagt tctgaaggcc ccgataggac agaaccccat gaccaagatc
     1441 gatggaaaaa tcatcaagta cgcggtgcag aagcccgagg acaaatctgc cgccgacaag
     1501 ccggcggagc ccaaggcgcc gccgccgccc gcgagcgagt ccgaggtggt acgcgacaag
     1561 aatggccgta ccgccaaagt catccgcatg cacgagaagc tcgagcgtcc ggagatgctg
     1621 gtcggctcga cttacaagat caagacaccg atctccgatc acgcgatgta cgtgaccatc
     1681 aacgacatcg tgctgaacga gggcaccgag tacgagcaac ggcggccgtt cgagatattc
     1741 atcaactcta agaacctcga tcactttcag tggatcgtgg ctctcacgcg catcatctcg
     1801 gcggtgttca ggaagggtgg cgatgtgacc ttcctggtgg atgagctgaa ggcggtgttc
     1861 gatccgcgcg gcggctactg gcagcccggc ggcaagttca tgccctcgat catcgccgag
     1921 ctcggccacg tgatcgagaa gcacctgcag gccatcggcc tgatgcgcag acgcgagctc
     1981 gacgtccacc agcagaagct ggtcgatgag aagcgcgcgg cgttcgaggc ccgcgccaag
     2041 cagaccgacg ccttcgccaa gacacacttc ccggagggcg cgcagctgtg cggccgctgc
     2101 agcaccgcgg cggtggtgat gatggatggc tgcatgacct gcctgaactg cggcgattcg
     2161 aagtgtggct gagcccgcga ccgattgttt gcgatgacca actacgactg acagaactgg
     2221 tgcgatttgc gaggcggccg aggctgcctc gccctttttc ggcttctcgc cccgctgccc
     2281 gcgcgcgcct cccgcctacc ccctgcggac gccccggccc gggacatagt caccccacgc
     2341 tacccaaaaa aaaccccgct tgcgcggggt gtcggtcgct tgcttgtgtg tggaggagtg
     2401 tcgttctcgt tgttatccgg tcttgcgcgg tatccggcga gccgggaggc tgctgcccga
     2461 tccgcgcttt tttcagttat ccctacgtgt cgtcttcctt ccccggcgtt tgcctctgct
     2521 ggttattaga aaagcaacac tcgtgccaag cgggggccat gacccggcca ggccctccac
     2581 gaccccccgg ggccccccca aatgtgacgc agtgcacgtt gtggacacgg cgcatggccc
     2641 gatcggctct cgcggggcag tctttgagct cgtcatgtcg gtgttctaga cagccgagtg
     2701 tcaaattact cgacgtattt cggggagcat tgtcaggtga ttcggcgacg cgctcaaacc
     2761 caagtgtctg aatctacgat ggaagtcggc tcttttcagg ggtgtaaatg acttcgacag
     2821 ggaggatttg gggctcacac aggccggtcg gcatccgcac ccgcgcggtg ggactcgcaa
     2881 cctggctgct cgcgctcgcc gcggcgctca gctgcgcgcg cgcgctggcc gccgaggatt
     2941 ccatgccgcg gccgccgcaa ctcgagcgcg acgtgcagtt ctgggtccgc gtgtattcgc
     3001 agatcgatac caatgcgggt ttcctgcacg atcagacgaa cctcggtgtg gtgtacgaca
     3061 cactgcattt cgcgcccgac gcgccgccct ccgagcgaca gcgccaggtg gagcaggcgc
     3121 gcgagcgcgt tgccgcgacg ttgcgccgca tcgcaggcag cggcgaggcg ccgttgtctg
     3181 ccgaagacca gcgcatccgt gacctgtggg gtgccgaagg gacgccggcg cggctgcgcg
     3241 cagcggtcga tgacatccgc ttccagctcg gacaatccga ccgcttccgc agcgggctga
     3301 tccgctcggg cgcgtgggaa actcacatcg ccgagacact cgccaatctc gggctgcccg
     3361 ccgagctcgc cgtgctgccg cacgtggagt cctccttcaa cccgagcgcc tactccaagg
     3421 tcggcgccgc gggcctgtgg caattcatgc gctcgaccgg ccggcgctac atgcgcatcg
     3481 acggcgcggt ggatgaccgg ctcgacccgt tccgctcgac cgaggccgca gcgcagctgc
     3541 tcgcttacaa ctaccgggtg ctcggcacct ggccgctggc gctgacggcc tacaaccatg
     3601 gcaccgcggg ggtgcggcgc gccaaggaga cgctcggcac cgatgacatc gtgcgcatcg
     3661 tgcgcagcta cagcagccgc accttcggat tcgcctcgcg caacttctac gtttcgtttc
     3721 ttgccgcgct cgagatcgac cgcaacccgg agaagtactt cgggtcgctg cagaaggaga
     3781 gcgaggcgca cttccaggag ctcgagctgc cggcgtacgt gtccgccacc tcactcgagc
     3841 acgtcttgaa gctcgatgcc ggcaagctgc gtgcgctgaa tcccgcgctc ctgcccgcgg
     3901 tctggaacgg cgcgcagcgt gtgccgaaag cttatcggct gcggttgccg gcggatggct
     3961 ccccgtggag cagccagctg ctcgcgcagc gtctgacgcc cggcgagatg ttcaccggcc
     4021 agcgcgctcc gccgcactat cgtgtgcagc gtggcgatac gctcgcggct ctcgccagcc
     4081 gctatggtgt gccgatcgag tcgctggcgg agctgaacca tctgcgcacc agcgcgcacc
     4141 ttcgtcccgg ccgtgtgctg acggtgccgg agaccacctc catcagtttg gcggcggcat
     4201 cggctgcgcc cgcctccgtt cc
//
LOCUS       DATDMP010000216         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1588259, whole genome shotgun sequence.
ACCESSION   DATDMP010000216 DATDMP010000000
VERSION     DATDMP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588259"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VI195_03670"
     CDS             complement(<1..554)
                     /locus_tag="VI195_03670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351502.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY6823511.1"
                     /translation="MAIKASEISELIRQRIENFEAVTEARNVGTIISVTDGITRIHGL
                     ADARYGEMLEFPGNTFGLALNLEQDSVGAVVLGEFEHLKEGDTVKTTGRILEVPVGPE
                     LLGRVVDALGTPLDGKGPVKAARTAPIERVAPGVIYRKSVSQPVQTGYKAIDSMVPIG
                     RGQRELIIGDRQTGKTAVAVDTII"
     gene            complement(582..1118)
                     /locus_tag="VI195_03675"
     CDS             complement(582..1118)
                     /locus_tag="VI195_03675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY6823512.1"
                     /translation="MADKVTIARPYARAAFEEARTHAGLEGWSEGLQLAASVVQDGRV
                     AALLGNPRVTPAELARLVTGIAGGKLGEAGANFIELLAVNRRLGCLPEIATLFDTFKD
                     EAQGVAHVTVTSATPLDAPQRESLTQALERRLERRVRLHCVTDPQLIGGAVLRSGDLV
                     IDGSLRTQLERIAYQLTA"
     gene            complement(1125..1598)
                     /locus_tag="VI195_03680"
     CDS             complement(1125..1598)
                     /locus_tag="VI195_03680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY6823513.1"
                     /translation="MVNINLTLIIQMVVFALLVWFTMRFVWPYIMAAVEERGRKIALG
                     LAAAEKGEQELAAARERADAVVREARERATQIIDHAQHRANDLLEQARGTASSEGARL
                     IAAAQQQIELDAAHARETLRRQVAGIAVGAAAKLLGREIDARAHADLLERLAAQI"
     gene            complement(1645..1917)
                     /gene="atpE"
                     /locus_tag="VI195_03685"
     CDS             complement(1645..1917)
                     /gene="atpE"
                     /locus_tag="VI195_03685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY6823514.1"
                     /translation="MEIAHIYGMTVIAVGLIFGMGALGTAVGFGILGGRFLEGVARQP
                     ELAPVLLVRMFIVAGLLDAVAFIGVAFALFFTFANPFVSAFQAVHH"
     gene            complement(1960..2817)
                     /gene="atpB"
                     /locus_tag="VI195_03690"
     CDS             complement(1960..2817)
                     /gene="atpB"
                     /locus_tag="VI195_03690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY6823515.1"
                     /translation="MTAAENGSGATEYILHHLTFLSNKTPQGIADFSVVNYDSVFFSV
                     LLAVAFGGAFYAAARRASVAAPGKFQLVVEIIVEFVDTLVKDTYHASSKLIAPLALTI
                     FCWVFLFNAMDVLPLDLLPNAAYVSGLAHLRIVPSTDLNVVFGLSITVFILIIAYSLK
                     MKGIRGFIGEFTLHPFRSRNWLVQVLFVPINLIMEVPAFLARPVSLALRLYGNMYAGE
                     MVFVLISLLTLSSGFHALASLGGWLSIVGSVAAGVIWTGFDLFVALLQAFIFGMLTIV
                     YLAHASEHQ"
BASE COUNT          551 a          981 c          959 g          473 t
ORIGIN      
        1 ttgatgatcg tgtcgacagc gacggccgtt ttgccggtct gacggtcacc gatgatcaac
       61 tcacgttggc cgcgtccgat cggcaccatg gagtcgatgg ccttgtagcc ggtctgcacc
      121 ggctggctca cggactttcg gtagatgacg cccggagcca cgcgctcgat cggggcggtg
      181 cgcgccgctt tcaccggtcc cttgccgtcg agcggcgtgc cgagcgcatc caccacgcgc
      241 ccgagcagtt ccggccccac gggcacctcg aggatccggc cggtggtctt gacggtatcg
      301 ccctccttga ggtgctcgaa ctccccgagc accaccgcgc cgaccgaatc ctgctcgagg
      361 ttgagcgcca ggccgaaggt gttaccgggg aactcgagca tctcgccgta gcgcgcgtcc
      421 gccagaccgt ggatgcgcgt gatgccgtcg gtgaccgaga tgatggtgcc gacgttgcgc
      481 gcctcggtga ccgcctcgaa gttctcgatg cgctgcctga tgagctcgga gatctcggat
      541 gccttgatgg ccatgtgcgt tcctgggggt gtcggttgcg gtcaggcggt gagctggtag
      601 gcgatgcgct cgagctgggt gcgcagcgag ccgtcgatca cgagatcgcc gctcctgagc
      661 accgccccgc cgatgagctg cgggtccgtc acgcagtgca gccgcacgcg ccgctcgagg
      721 cgccgctcga gcgcctgggt gagcgactcg cgctgcgggg catcgagcgg cgtcgccgag
      781 gtcacggtga cgtgggccac gccctgcgcc tcgtccttga aagtatcgaa cagcgtcgcg
      841 atctccggca ggcagccgag gcggcggttg acggcgagca gctcgatgaa gttcgcgccc
      901 gcctcgccga gcttgccgcc ggcgatgccg gtcaccagcc gcgccagctc cgccggcgtg
      961 acccgcgggt tgccgagcag cgccgccacg cggccatcct gcaccacgct cgccgcgagc
     1021 tgcagtccct cggaccagcc ctcgaggcct gcgtgcgtgc gcgcttcctc gaaggccgcg
     1081 cgggcatagg gtcgggcgat ggtgaccttg tcggccatgg gtgctcagat ctgcgccgcg
     1141 aggcgctcga gcagatccgc gtgggcgcgc gcatcgatct cgcggccgag gagcttggcg
     1201 gcggcgccca ccgcgatgcc ggccacctgg cggcggagag tctcgcgcgc gtgcgcggcg
     1261 tcgagctcga tctgctgctg cgcggcggcg atcaggcgcg cgccctcgct gcttgccgtg
     1321 ccgcgggcct gctcgagcag gtcgttggcg cggtgctggg cgtggtcgat gatctgcgtg
     1381 gcccgctcgc gcgcctcgcg caccacggcg tcggcacgct cgcgcgccgc ggcgagctcc
     1441 tgctcgccct tctcggccgc cgcgagtccg agggcaatct tgcgtccccg ttcctcgacg
     1501 gccgccatga tgtaaggcca gacgaacctc atcgtaaacc agacgagcaa cgcaaagacg
     1561 accatctgga tgatgagtgt caggttgatg ttcaccatcg actcctaaag tagcgcacga
     1621 aggtcacgca tcgcggcaga agcctcagtg gtgcactgcc tggaacgcgg acacgaacgg
     1681 attggcgaac gtgaaaaaga gagcgaacgc cacgccgatg aacgctaccg cgtcgagcaa
     1741 accagcgacg atgaacattc tgaccagtag gacgggagcg agctctggct ggcgggcaac
     1801 gccttcgaga aagcgaccac caaggattcc gaatccgacc gccgtaccga gcgcgcccat
     1861 cccgaaaatg agtccgacgg caatcacggt catgccatag atgtgtgcaa tttccattca
     1921 gccctcctca agagaaaaat cgacgcagtc tgcggggctc tattgatgct ccgaggcatg
     1981 cgcgagatac acaatggtca gcattccgaa tatgaaggcc tgcagcagcg ctacaaacag
     2041 gtcgaagccg gtccagatga ctccggcggc gacggacccg acgatgctca gccagccccc
     2101 gagcgatgcg agcgcgtgga atccgctcga gagcgtgagc aatgaaatca gcacgaatac
     2161 catctccccg gcgtacatat tgccgtagag acgtaaggcg agcgagacag gacgcgcgag
     2221 gaacgccgga acttccatga tgaggttgat cggcacgaac aggacctgca cgagccagtt
     2281 gcgactcctg aacggatgca gcgtgaactc gccgatgaag ccccggattc ccttcatctt
     2341 caagctgtac gcaatgataa gaatgaacac ggtgattgac aggccgaaaa cgacgttcag
     2401 gtccgtgctg ggtacgatgc gcagatgcgc cagccccgac acgtacgctg cgttgggcag
     2461 cagatcgagc ggcagcacat ccatcgcgtt gaacaggaac acccagcaaa agatcgtgag
     2521 agcaagcggc gctatcagct tgctgctggc gtgataggtg tcctttacga gcgtatcaac
     2581 gaactcgacg attatctcca caaccagctg gaacttgccc ggcgccgcca cactcgcccg
     2641 gcgtgccgcg gcgtagaaag cacccccgaa cgccacggca agcagcacgg aaaagaacac
     2701 gctgtcgtaa ttgacaaccg aaaaatcggc gatcccctgc ggcgtcttgt tcgacaggaa
     2761 agtcaggtgg tgcaggatgt attcggtcgc cccggagccg ttctcggccg ctgtcatcag
     2821 cgtcagcatt ctcccgctcc cgcgctcacg cgccccggcg cgtgccaccc aagctcggca
     2881 gcgcgcccac cagcgcgatc cagtacacga ggtaggtcgc ggcgaaaccg ccgagcaacg
     2941 ccaccggtat caccctcacc atcc
//
LOCUS       DATDMP010000217         4117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1255603, whole genome shotgun sequence.
ACCESSION   DATDMP010000217 DATDMP010000000
VERSION     DATDMP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255603"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..862
                     /locus_tag="VI195_03695"
     CDS             362..862
                     /locus_tag="VI195_03695"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY6823516.1"
                     /translation="MKPFRVLFLCTGNSARSILAEACLNAAGQEKFIACSAGSRPVGK
                     VNPFALQLLERNAIPTRGLRSKGWDEFAAPGAPAIDFVITVCDNAAGETCPLWPGNPV
                     TAHWGVPDPAAVEGSDDQKRRAFFDAFAILSARIRALMALPVETLDARALKKALDRIG
                     SSIQEH"
     gene            926..2128
                     /locus_tag="VI195_03700"
     CDS             926..2128
                     /locus_tag="VI195_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY6823517.1"
                     /translation="MSHVMNTYARQPVAFVRGEGVWLWDEQGRKYLDALAGIAVNTLG
                     YAHPRLTKALTERIKSGVLHTSNLWRIPDQEAAADRIAEITGLDEVFFCNSGLEANEA
                     AIKIARKYGHERGIAEPAIIVMEKAFHGRSLATLSATGSRKVQAGFEPLVSGFVRVPL
                     NDLAAVRTVAANNRNIAAVMIEPIQGEGGINVSRLDYLRELKQICDEHQWLFICDEVQ
                     CGLGRTGKWFVYQHAGFLPDVVPLAKGLGSGVPVGACVVGGRALGVFKPGNHGSTFGG
                     NPLAMTAVVATLDTIKQEGLMENAARVGKVISDSLRSELGAHEGVAEIRGMGLMLGLE
                     LRRGCGELVKQALEAGLVINVTVDNVVRMLPPLVMSEAEGRQVAERLVPLVREFLGRA
                     AQPKAAAH"
     gene            2154..3065
                     /gene="argF"
                     /locus_tag="VI195_03705"
     CDS             2154..3065
                     /gene="argF"
                     /locus_tag="VI195_03705"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY6823518.1"
                     /translation="MSAVRHFLQLKDLSREEFEHLFARTRWIKDKFKRYERYWPLEDR
                     TLAMIFEKQSTRTRLSFEAGMHQLGGAAIFLSTRDSQLGRGEPVEDAAQVVSRMCDVV
                     MIRTFEQEIVERFARHSRVPVINGLTNEYHPCQVMGDIYTYIDHRGDIRGRTVAWIGD
                     SNNVCNTWLQAAEAFDFKINISTPPGYEIEPERKNRHCEAFTDPMKAARGADLVTTDV
                     WTSMGFESENEERKKAFEDWQVDAEMMRAAKKDALFMHCLPAHRGEEVTADVIDGPQS
                     VVWDEAENRLHTQKALLEFLLLGKVKP"
     gene            complement(3070..>4117)
                     /locus_tag="VI195_03710"
     CDS             complement(3070..>4117)
                     /locus_tag="VI195_03710"
                     /inference="COORDINATES: protein motif:HMM:NF005720.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEY6823519.1"
                     /translation="DVYSEPRGLRAGYLWPQYSVHRGGLQMLLYRAVLQRLGPDGVRT
                     GVWVTGYRNHAAGSGVTALVEAREGGRQEIEGSLLIGADGLHSAVRAQMHPGQPPIHW
                     GGAIMWRGVTKGVPIRTGASFVGFGTHRHRVVFYPISRRGPKDGLADINWIAEITVDN
                     AKGWRDGDWNRKVKHEDFLHYFADWTHDWIDIPAMLRGAADVYEYPMIDRDPVPTWVD
                     GRVALLGDAAHVMYPTGSNGGTQAIIDARVLGASLLAHGVTPAALRDYDQRLCKDVSA
                     LVLRNRGAGPFGLLSLLDERCGGVFDDIESVIPRAERDEFMRGYKAAAGLAMEKLNAS
                     PPILARGAATTYPS"
BASE COUNT          727 a         1369 c         1347 g          674 t
ORIGIN      
        1 gctgcggcgc ggcatcaagc ttctttggag atcgaaagcg agctcggaag ggggagccgg
       61 ttcacgctgc gctttccggg taaacgcacc attgcggcgg agccggcggg agagagagca
      121 cctgcctccc ggtaaccact cccttggagg caggcgctct ttacggcatt acttcttctt
      181 tttggacgac ttctttgctt ttttgcgttt tgccactttt ggtctccctg agggtcccat
      241 catcggaaaa acccgatgat tagtctcgtg tgtactggat cgcgcatgtt cacgcaacta
      301 gttgaagcag ttgaatgcgg ttatgcgctc atgtctgtgg caaaaaaaac acactgatgc
      361 aatgaaaccg ttccgcgttc tctttctttg caccggcaac tcggcgcgat cgattctcgc
      421 cgaggcctgc ctgaacgccg ccggacaaga aaagttcatt gcctgtagcg cgggcagccg
      481 tcccgtcggc aaggtcaacc ctttcgcgct gcagcttctc gaaaggaacg caatcccgac
      541 gcgcggcctg cgctcgaagg gatgggatga gtttgcggcg ccgggcgcgc ccgcgatcga
      601 cttcgtcatc accgtctgcg acaacgccgc aggcgagacc tgcccgttat ggcccggcaa
      661 cccggtcact gcgcactggg gcgtgccgga cccggccgca gtcgaaggca gcgacgatca
      721 gaagcgaagg gctttttttg acgcattcgc catcctctcc gcccgcatca gggcgctcat
      781 ggcgcttccg gtcgagaccc tcgatgcgcg cgcgctgaag aaggcgctgg accggatcgg
      841 ctcgtctatc caagagcatt aacccctgat aggattgaaa aaacggcggc atcgagccgc
      901 cgttttgcat ttagggagag cgcgcatgtc ccacgtcatg aacacctacg cccgccagcc
      961 ggtcgctttc gtgcgcggcg aaggcgtctg gctctgggac gagcagggcc gcaagtacct
     1021 cgacgcgctc gccggcatcg ccgtgaacac gctcggctat gcgcacccca ggctcacgaa
     1081 agcgctgacc gagagaataa agagcggcgt cctgcacacc tcgaaccttt ggcgcatccc
     1141 ggaccaggaa gccgccgccg accgcatcgc cgagatcacc ggcctcgatg aggtgttctt
     1201 ctgcaattcc gggctggagg cgaacgaggc ggccatcaaa atcgcgcgca aatacggcca
     1261 cgagcgcggc atcgccgagc cggccatcat cgtaatggag aaggccttcc acggccgctc
     1321 gctcgccacg ctctcggcca ccggaagccg caaggtgcag gcaggattcg agccgctggt
     1381 atcaggcttc gtgcgcgtgc cgcttaacga cctcgcggcg gtgcgcacgg tggcggccaa
     1441 caaccgcaac atcgccgcgg tcatgatcga gcccatccag ggcgaaggcg gcatcaacgt
     1501 ctcgcgcctg gactacctgc gcgagctgaa gcagatctgc gacgagcacc agtggctctt
     1561 catctgcgac gaggtgcaat gcggcctggg ccgcaccggc aagtggttcg tctaccagca
     1621 tgcggggttc ctgcccgacg tggtgccgct cgccaagggg ctcggctcgg gcgttccggt
     1681 cggcgcttgc gtggtgggcg ggcgcgccct cggggtcttc aagccgggca accacggctc
     1741 cactttcggc ggcaacccgc tcgccatgac cgcggtggta gcgacgctgg acaccatcaa
     1801 gcaggaaggc ctgatggaga acgccgcgcg ggtcggcaag gtgatcagcg actcgttgag
     1861 gagcgagctc ggagcccatg aaggcgtggc cgagatccgc ggcatgggcc tcatgctcgg
     1921 cctggagctt cgccgcggct gcggcgagct cgtcaagcag gcgctcgaag ccggcctcgt
     1981 gatcaacgtc accgtggaca acgtcgtgcg catgctgccg ccgctcgtca tgagcgaggc
     2041 cgaaggccgt caggtcgccg agcggctggt gccgctcgtg cgcgagttcc tgggtcgcgc
     2101 cgcgcagccc aaggccgcgg cgcactgagc gccgcccgaa gcgccccgat cccgtgtccg
     2161 cggtccgcca cttcctgcag ctcaaggacc tctctcgcga ggagttcgag catctcttcg
     2221 cgcgtacgcg ctggatcaag gacaagttca agcgctacga gcgctactgg ccgctcgagg
     2281 accgcacgct cgcgatgatc ttcgagaagc agtcgacccg cacccgcctc tccttcgagg
     2341 cgggcatgca ccagctcggc ggcgccgcga tcttcctctc cacccgcgac tcgcagctcg
     2401 ggcgcggcga gccggtggag gacgccgcgc aggtcgtctc gcgcatgtgc gacgtcgtga
     2461 tgatccgcac cttcgagcag gaaatcgtcg agcgcttcgc ccggcattcg cgcgtgccgg
     2521 tgatcaacgg cctcaccaac gagtaccacc cctgccaggt gatgggcgac atctacacct
     2581 atatcgacca taggggcgat atccgcgggc gcaccgtcgc ctggatcggc gactcgaaca
     2641 acgtctgcaa tacctggctg caggcggccg aggcgttcga cttcaagatc aacatctcca
     2701 cgccgccggg ctacgagatc gagcccgagc gcaagaacag gcactgcgag gccttcaccg
     2761 atccgatgaa ggcggcaagg ggcgccgacc tcgtcaccac cgacgtctgg acctcgatgg
     2821 gcttcgagtc cgagaacgag gaacgcaaga aggccttcga ggattggcag gtcgacgccg
     2881 agatgatgcg cgcggcgaag aaggacgcgc tcttcatgca ttgcctgccc gcgcaccgcg
     2941 gcgaggaagt caccgccgac gtgatcgatg gcccgcagag cgtggtttgg gacgaggccg
     3001 agaaccgcct gcacacccag aaggcgctgc tcgagttcct gctcctgggg aaagtgaagc
     3061 cataagggct caactagggt aagtcgttgc cgcgccgcga gcgaggatcg gcggggaggc
     3121 attgagtttt tccatggcaa ggcctgccgc cgccttgtag ccacgcatga actcgtccct
     3181 ctccgcgcga ggaatcaccg actcgatatc gtcgaacacc cccccgcagc gctcatcgag
     3241 caggctgagc aggccgaacg gcccggcgcc ccggttgcgc aacacgagcg ccgagacgtc
     3301 cttgcacaat cgctggtcgt agtcgcgcaa cgctgccggc gtcacgccgt gcgcgagcag
     3361 cgaggcgccg agcacccgcg catcgatgat ggcctgggtg ccgccgttgg agccggtggg
     3421 atacatcacg tgcgcggcgt cccccagcag ggcgacgcgc ccgtccaccc aggtcggcac
     3481 cgggtcgcgg tcgatcatcg ggtactcgta cacgtccgcg gcgccacgaa gcatggcggg
     3541 gatgtcgatc cagtcgtggg tccagtccgc gaagtaatgc aggaagtctt cgtgcttcac
     3601 cttccggttc cagtcgccgt cgcgccagcc tttggcattg tcgacggtga tttccgcgat
     3661 ccagttgatg tcggcgaggc cgtccttcgg gccgcgccgc gagatggggt agaacacgac
     3721 gcggtggcgg tgggtgccga agccgacgaa ggacgcgccg gtccggatcg gcacgccctt
     3781 ggtgacgccg cgccacatga tggcgccgcc ccaatggatg ggcggctgcc ccggatgcat
     3841 ctgggcgcgc acggccgagt gcaggccatc ggcgccgatg aggagtgagc cttcgatttc
     3901 ctgccgtcca ccctcgcgcg cttcgacgag cgccgtcacg cctgagccgg cggcgtggtt
     3961 gcgatagccg gtgacccaga cgccagtgcg caccccatcc gggccgagcc gctggagcac
     4021 cgcgcgatag agcagcatct gcaggccgcc gcgatgcacc gagtactgcg gccagaggta
     4081 gcccgcccgc aggccgcgcg gctcggagta gacgtcc
//
LOCUS       DATDMP010000218         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2032625, whole genome shotgun sequence.
ACCESSION   DATDMP010000218 DATDMP010000000
VERSION     DATDMP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032625"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VI195_03715"
     CDS             <1..962
                     /locus_tag="VI195_03715"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY6823520.1"
                     /translation="RRPPRRAPRARRAAAVPTLRSWLFVPGDSERKQEKALASAADAV
                     ILDLEDSVAAAQLTAARRRVAELLRSVRARHRPQLWVRANAPSSDLLREDLEAVFAGG
                     APDGIVLPKVSGAAEIAPVARRLAALESRLGMERGGTRLLIIATETPAGVLSLAQYPA
                     ALGQVPSIRQRIAGLTWGAEDLSTALGALAQRDAGGALTFTFELARTHCLLAAAALGT
                     QAIDGVCTDFRDAARLQHELDAARRDGFSGKLAIHPDQIATINAAFAPTDAEREHARR
                     VVAAFASAPAAGVVSLDGRMIDRPHLLQAQRILASAQQDQDRT"
     gene            959..1342
                     /locus_tag="VI195_03720"
     CDS             959..1342
                     /locus_tag="VI195_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823521.1"
                     /translation="MKLRIKGPTLRLRLTQAEIRALLDEGQVEERVPFAAGASLIYRL
                     KRDAAAGEIAASFHDGVLEILIPEGNAREWCLTPLVTLAHEQALANGSLRITLEKDFA
                     CLAPREGEDESDNFPHPEAGSGRTC"
     gene            complement(1350..1964)
                     /locus_tag="VI195_03725"
     CDS             complement(1350..1964)
                     /locus_tag="VI195_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY6823522.1"
                     /translation="MKSTDGSARSADGLYAVVLAAGASTRFGSAKQLVRVAGRPLLHT
                     AVTRAAEVTGTALIVVLGSGAAQLAPLLKHSPGSVVVNQEWREGLASSIRAGVTRLPA
                     ACSAVMLLLADQAAVTADDLRRLAGSWRKQPRHIAAALYDGVCGVPAIFPRSSFRALT
                     ELRGDSGARALLLRNPDRVVRVPMPSAGIDVDTPEDLLALEAPK"
BASE COUNT          320 a          744 c          767 g          295 t
ORIGIN      
        1 cgcggcggcc gccgcgccgt gcgccgcgcg cacggcgcgc ggcagcggtg ccaacgctgc
       61 gctcgtggct cttcgtcccc ggtgacagcg agcgcaagca ggagaaggcg ctggcgagtg
      121 ccgccgacgc cgtgattctc gatctcgagg actcggtggc cgcggcgcag ttgaccgcgg
      181 cgcggcggcg cgtcgccgag ctgctgcgct cggtgcgcgc gcgtcaccga ccgcagctgt
      241 gggtgcgcgc gaatgcgcca tcgagcgatc tgctgcggga ggacctggag gccgtcttcg
      301 ctggcggcgc gcccgacggc atcgtcctgc cgaaggtgtc gggtgccgcg gaaatcgcac
      361 cggtcgcgcg gcggctggcc gcgctcgagt cgcgcctcgg catggagcgg ggaggcacgc
      421 ggcttctcat catcgcgacc gagactcctg cgggtgtgct ctcgcttgcg cagtaccccg
      481 cggcacttgg ccaggtgccg agcatccgcc agcgcattgc cggcctcacc tggggcgcgg
      541 aggatctcag caccgccctt ggggccctgg cccagcgcga cgccggcggc gcactcactt
      601 tcacctttga actcgcgcgc acgcactgcc tgctggcagc ggcggcgctc ggcacgcagg
      661 ccatcgatgg cgtatgcacc gactttcgcg acgcggcgcg gctgcagcac gagctggatg
      721 cggcgcggcg cgatggcttc agcggtaagc ttgcgatcca tcccgatcag attgccacga
      781 tcaacgccgc gtttgcgccg accgacgccg agcgcgagca tgcacggcgg gtggtcgcgg
      841 cgttcgccag cgcaccggcg gcaggggtgg tcagcctcga tgggcgcatg atcgaccggc
      901 cgcacctgct gcaggcgcag cgcattctcg cgagtgcgca acaggaccag gaccggacat
      961 gaaactgagg atcaagggac cgacgctgag attgcgcctg acgcaggctg agattcgggc
     1021 tctgctcgat gaaggccagg tcgaggagcg ggtgccattc gcggctggcg cgagtctcat
     1081 ctatcggctc aaacgcgacg ccgcggcggg cgaaattgcc gcgagcttcc acgatggagt
     1141 gctcgagatc ctcatccccg agggcaacgc gcgcgagtgg tgtctcactc cgctcgtgac
     1201 gctcgcgcac gagcaggccc tggcgaacgg ctcactgcgc atcaccctgg agaaggattt
     1261 cgcctgcctc gcaccgcgcg aaggcgagga cgagtccgac aactttcccc accccgaggc
     1321 aggcagcggc aggacctgct agccgaaact cacttcggcg cttcgagtgc gagcagatcc
     1381 tccggcgtat ccacgtcgat gccggcgctc ggcatgggca cgcgcaccac ccgatccgga
     1441 ttgcgcagca acagcgcgcg cgcgccgcta tcgccacgca gctcggtgag tgcacgaaac
     1501 gagctgcgcg ggaagatggc cggaacgccg cagactccgt cgtagagtgc cgccgcaatg
     1561 tgccggggct gcttgcgcca gctgccggcg agccgccgta gatcatctgc ggtgaccgcg
     1621 gcctgatcgg ccagcagcag catgaccgcg ctgcacgcgg ctggcagccg cgtcacgccg
     1681 gcgcggatcg agctggcgag gccctcgcgc cattcctggt tgacgacgac cgagccgggg
     1741 gagtgcttga ggagcggggc cagctgcgcc gcgcccgagc ccaacaccac gatgagcgcg
     1801 gtgccggtca cctccgcggc acgcgtgacg gccgtgtgga gcagcggccg gcccgcgacg
     1861 cgtaccagtt gcttggccga gccgaagcgg gtcgaggcgc cggcggcgag cacgacggcg
     1921 tacaagccat ccgcgcttcg agcggagccg tccgtcgact tcacgcgcgc tccgtggccg
     1981 tgccgagtgt cacacccggc ggcacctgtg ccgccgccag cgcatagcgg cgccgctctt
     2041 cgaggatcga gtcggcatcg ataccgccca ggatgcgcca gtcgcccgcg cgggtgttgg
     2101 aagcgagcac ggcttcgaga gcatcg
//
LOCUS       DATDMP010000219         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2032904, whole genome shotgun sequence.
ACCESSION   DATDMP010000219 DATDMP010000000
VERSION     DATDMP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032904"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..601
                     /locus_tag="VI195_03730"
     CDS             131..601
                     /locus_tag="VI195_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY6823523.1"
                     /translation="MSTPALDRLDRRILEELQADARISNQELAKRVGLSAAPCWRRLR
                     RLEQAGFIAGYATLLKAPAIGLPILAYAVVSLENHHPESVRQFDQLVMARPEVLECHS
                     MSGANDYLLRIVAASMEAYEHFLSTQLLQLQAVRSVNTSFVLRTKKFTTRLPVG"
     gene            complement(576..2339)
                     /locus_tag="VI195_03735"
     CDS             complement(576..2339)
                     /locus_tag="VI195_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823524.1"
                     /translation="MPARTTATFLAVLFGLAGAAWGDTSSEMASMPQRPATVADWAQG
                     AQLFQGLGDFHRAVTTSSPQAQQYFDQGMRYMWAFNHDEATRSFAKAAELDPGCALCY
                     WGVALTVGPNYNRPAMEEPRARVAAEALHKAQENAAHASAVEQGLIAALATRYPTAAP
                     LGPGNLEPVVRAYAAAMRELAQRFPDDLDLQTLCAEAEMNVHAWKLWSADGAPAEGTA
                     DIQARLEAVLRRDPKHPGANHYYIHTMEASHHPEKALAAAERLPGMMPAAGHLEHMPA
                     HIMQRVGSYEQAAEANRRGISADRAYLATTSPPDYYAMYLAHNYSFLAFSAAMEGRKA
                     ETLAAVQSVVMTVPLDMVLAMGDSGWNLSQQYAALVRFGLWDEAIALGAPDARSPGLT
                     AGYLFSRGVALAARGRLEDARAALTQLRQLGAAIPAESAAGFNSLHGILAVAEPILAA
                     RIAATERRNDEALAQLRAAVAAQDKLAYNEPADWFFPARHLLGAQLLIARRPAEAERV
                     YREDLQHNPGNGWALYGLATALREQGKTTSAARVTRDFDSAWRHADIRLQSSAFWFAG
                     ADNLSCECQRVSMLSRPEDGS"
     gene            complement(2359..>3104)
                     /locus_tag="VI195_03740"
     CDS             complement(2359..>3104)
                     /locus_tag="VI195_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6823525.1"
                     /translation="ATVMALGRTLGKIPVLAGNCDGFIGNRMLMFYGSEAEFLLEEGA
                     TPEQIDRVMEGFGFAMGPLAVRDLAGNDVGFLIRKGRRLPADERWSPILERIVAAGRL
                     GQKSGKGFYRYEGRTRIVDPEVTALIEEVSRELGVRRRTIPDEEILERLLHPLVNEGA
                     RILEDGIAIRASDIDVVYVYGYGFPAYKGGPMFWAEQAGLARVIETMRRLAPTHGARW
                     RSAPLLERLVAANQGFGAARSGVGVQKNT"
BASE COUNT          501 a         1062 c         1030 g          511 t
ORIGIN      
        1 gggcatggat cagacctccg aggggtgcgc gcgcagctta tgcgcgatca cgtgcaatta
       61 ccttgcttct attgccctat agaccatcaa ttcagcaata ttcttgcgtg atcagttgag
      121 gtagacagcc atgagcaccc cggcactcga tcgtctcgac cggcgcattc tggaagagct
      181 gcaggccgat gcacgcatct ccaaccagga gctcgccaag cgtgtcgggc tctccgccgc
      241 accctgctgg cgccgtctgc gacggctcga gcaggcgggc ttcatcgccg gttacgcgac
      301 gctgctgaag gctcccgcca tcggtctgcc gattctcgcc tacgcggtcg tgtcactcga
      361 gaaccatcat cccgagtccg tgcgccagtt cgatcagctg gtgatggcgc ggccggaagt
      421 gctcgaatgt cactcgatga gcggggcgaa cgactacctg ctgcgcatcg tggccgccag
      481 catggaggcc tacgagcact tcctttccac acagctgctg cagctgcagg cggtgcgctc
      541 ggtcaacacg agcttcgtgc tgcgcaccaa gaaattcacg acccgtcttc cggtcggctg
      601 agcatcgaca cgcgctgaca ctcgcagctg agattatccg cgcccgcgaa ccaaaacgcg
      661 gaagactgca gccggatgtc ggcgtgccgc caggcgctgt cgaagtcgcg ggtgacgcgc
      721 gcagcgctcg tggtcttgcc ctgctcgcgc agcgccgtgg cgagaccgta cagtgcccag
      781 ccgttgccgg gattgtgctg caggtcctca cggtagacgc gctcggcttc cgccgggcgg
      841 cgggcgatga gcagctgcgc accgagcagg tggcgtgcgg gaaagaacca gtcggccggc
      901 tcgttgtagg cgagcttgtc ctgggccgcc accgccgcgc gcaactgcgc gagcgcctca
      961 tcgttgcgcc gctcggtcgc ggcaatgcgc gccgcgagta tcggctcggc caccgcgaga
     1021 atgccgtgca gcgagttgaa tccagccgcc gactcggcgg gaatggcggc gcccagctgc
     1081 cgcagctgcg tgagcgccgc gcgcgcatcc tcgagccgcc cgcgcgctgc gagcgccacg
     1141 ccgcgactga agagatatcc ggcggtgagc ccgggcgagc gcgcatccgg cgcgccgagc
     1201 gcgatcgcct cgtcccacag gccgaagcgc accagcgcgg cgtactgctg agagagattc
     1261 caaccggagt ctcccatggc gagcaccatg tcgagcggca ccgtcatcac gacgctctgc
     1321 acggcggcca gcgtttcggc cttgcgtcct tccatggcgg ccgagaatgc caggaaggaa
     1381 taattgtgcg cgagatacat cgcgtagtaa tcgggcggtg aggtggtggc gaggtacgcg
     1441 cggtcggcgg aaatgccgcg gcggttcgcc tcggctgcct gctcgtatga gcccacgcgc
     1501 tgcatgatgt gcgccggcat gtgctcgaga tgaccggccg ccggcatcat gccgggcagc
     1561 cgctcggcgg cggcgagcgc tttttcgggg tggtgtgagg cctccatcgt atggatgtag
     1621 tagtgattgg cgcccgggtg cttcggatcg cggcgcagga ccgcttcgag ccgcgcctgg
     1681 atgtccgcgg tgccctcggc gggcgcgcca tcggcactcc acagcttcca cgcgtgcacg
     1741 ttcatctcgg cctccgcgca cagcgtctgc aggtcgaggt catccggaaa gcgctgggcg
     1801 agctcgcgca tcgcggccgc gtaagcgcgc accaccggct cgagattccc cgggccgagg
     1861 ggtgctgcgg tcggatagcg cgtggcgagc gccgcaatca gcccctgctc gaccgccgat
     1921 gcgtgtgcgg cgttctcctg tgccttgtgc agagcctccg cggccacgcg cgcacgcggc
     1981 tcctccatcg ccggccggtt gtagttcggg ccgacggtga gcgccacgcc ccagtaacag
     2041 agcgcgcaac cgggatcgag ctccgccgcc ttggcgaagg agcgcgtcgc ctcgtcgtga
     2101 ttgaatgccc acatgtagcg catgccctgg tcgaagtact gctgcgcctg cggcgaagag
     2161 gtggtcacgg cgcggtgaaa gtcgccgagt ccctggaaga gctgcgcgcc ctgggcccag
     2221 tccgccaccg ttgccggtcg ttgcggcatg gacgccatct cggaggaggt atcgccccac
     2281 gctgcgcccg ccaggccgaa aagaactgcc aaaaaagtgg ccgtggttcg tgccggcatc
     2341 aatccctcca ttcgctgctc aggtgttttt ttgcacgccc acgccggagc gggcggcgcc
     2401 aaagccctga ttcgcggcga ccagtcgctc gagcagcggt gccgaacgcc agcgcgcacc
     2461 gtgcgtgggt gcgagccgcc gcatggtctc gattacgcgc gcgagccccg cctgctcggc
     2521 ccagaacatc gggccgccct tgtacgccgg aaaaccgtaa ccgtagacat acactacatc
     2581 gatgtcggaa gcgcgtatcg cgatcccgtc ctccagaatg cgcgccccct cgttgacgag
     2641 cgggtgcagc agacgctcga ggatctcctc atcggggatc gtgcggcgcc gcacgccgag
     2701 ctcgcgcgac acctcctcga tgagcgcggt gacctccggg tcgacgatgc gcgtgcgccc
     2761 ctcgtaacgg taaaaaccct tgccactctt ctgaccgagt ctgccagcgg ccacgatgcg
     2821 ctcgaggatc ggcgaccagc gttcatccgc gggcagcctg cggcctttgc ggatgaggaa
     2881 accgacatcg ttgcccgcga gatcccgcac tgcgagcggc cccatggcaa acccgaagcc
     2941 ctccatcacc cgatcgatct gctcgggggt ggcgccctcc tccagcagga actccgcctc
     3001 gcttccgtag aacatgagca tgcggttgcc gatgaaccca tcgcagttac cggcgagcac
     3061 cggaatcttg ccgagggtcc tgccgagcgc catgacggtg gcga
//
LOCUS       DATDMP010000220         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1256086, whole genome shotgun sequence.
ACCESSION   DATDMP010000220 DATDMP010000000
VERSION     DATDMP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256086"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VI195_03745"
     CDS             complement(<1..195)
                     /locus_tag="VI195_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6823526.1"
                     /translation="MPEAQPIVFLLDDEQPVVVSLARMLQTSGFAVRYWTSAAEFLEA
                     HDADAPGCLVTDLRMPGMGGL"
     gene            489..1280
                     /locus_tag="VI195_03750"
     CDS             489..1280
                     /locus_tag="VI195_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823527.1"
                     /translation="MALAMVAADAVQAGPLDDTFDLSLGAFILSTKTTVRADGSPAPG
                     APVEIGTPINVERELDIADRTSFRLDGYWRFFERHKLRVMYFDESRSADKSITDEIVF
                     RGQTYPVNTQVNTRFDTLVAELAYEYAFLRGEHYELAGSLGIHELTFKLQLTAMGANT
                     NTQQSARADVSGPLPVIGVHYIWEFYPRWSLDAMFQFFALKYQQYDGNLQDYNATVYY
                     MPWKNFGFGAGWNQFRTNVNIDNASYDGNLNWRYGGVRLFFKASY"
     gene            1332..1943
                     /locus_tag="VI195_03755"
     CDS             1332..1943
                     /locus_tag="VI195_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY6823528.1"
                     /translation="MLGLGAIFTLFFVTLGPFKLLGPFAQETEELSPAALRGIAVRAF
                     VVGVVAVIVGGYLGTVLAAKWNVSTPAIEIATGLILLLVAIRIVMAPYDPPRSRSPLP
                     PSPMAATLRLTFPFVVTPYGIAGLIAVLDLAADGKTTASVYALLIVVMLLNLLAMLFV
                     REITRGPVLLILQVLGAVLGVLQFALAIQIIIRGLRDLNVLPA"
BASE COUNT          384 a          687 c          646 g          393 t
ORIGIN      
        1 gagaccgccc atgcccggca tccgcagatc ggtgaccagg cagccgggcg catccgcgtc
       61 gtgcgcttcc aggaactccg ccgccgaggt ccagtagcgc acggcaaagc cactcgtttg
      121 cagcatgcgc gccagactca ccacgaccgg ttgctcatca tccagcagaa agacgattgg
      181 ctgcgcctca ggcatcacca attcccttcg tcatcggcgc gaaagacatc cccgcagcgc
      241 attcggctat tgttctgagt aagtcgagcc actcgattat gcccttcacg ctgcgcgcct
      301 cgccagagcg tggccggcgt gcaaagatct ttagcttaag cctggcggtt gtgcgtggtc
      361 agaccaaggt cctatcgcgc aactacaccg gagatccata taaaacgcgc atgccggccc
      421 tgaggaacac gagcatgcgg cacaaagcgc ggtcgcgcac cgcagcttgc ccccatcgag
      481 ctctgttcgt ggcgctggct atggtcgcgg cggacgcggt gcaggcgggg cctctcgatg
      541 acaccttcga cctcagtctc ggggctttta tactcagcac gaaaaccacc gtgcgcgccg
      601 atggctcacc agcacccgga gcgccggttg agatcggcac cccgatcaat gtcgagcgag
      661 agctcgacat cgccgacagg accagcttcc ggctcgatgg atactggcga ttcttcgagc
      721 ggcacaagct ccgcgtgatg tatttcgatg agagccgctc ggcggacaag agcatcacgg
      781 acgagatcgt ctttcgtgga cagacctatc ccgtcaacac tcaggtcaac acgcgcttcg
      841 atacgctggt ggcggagctt gcctacgaat acgcctttct gaggggcgag cattacgagc
      901 tggccgggtc gctcggcatt cacgagctga ccttcaagct gcagctcacc gccatgggcg
      961 cgaacaccaa cactcagcag tccgcgcgcg cggacgtgag cggacctctg ccggtgatcg
     1021 gagtgcacta catctgggag ttctatccgc gctggagcct cgatgcgatg ttccagttct
     1081 tcgcgctgaa gtaccagcag tacgacggca acctgcagga ctacaacgcc acggtgtatt
     1141 acatgccctg gaagaacttc ggcttcggcg ccggctggaa ccagttccgg acgaacgtca
     1201 acatcgacaa cgccagttac gacggcaacc tcaactggag gtacggcggc gtgcggcttt
     1261 tcttcaaagc atcctactga aggcggctcg cggcgctgaa ggcgcggatt ggaccgctgg
     1321 cggagacgtc catgctggga ttgggagcga tcttcacgct ttttttcgtg acgcttgggc
     1381 cgttcaagct gctcggcccc ttcgcccagg agacagagga gttgagcccc gctgcgctgc
     1441 gcgggatcgc cgtgcgggca ttcgtcgtcg gcgtcgtcgc cgtgatcgtg ggggggtacc
     1501 tcggcacggt actcgcagcc aagtggaatg tgtcgacccc ggcgatcgag atcgcgaccg
     1561 ggctcatcct gctgctcgtg gcgatacgca tcgtcatggc accgtatgac cccccacgga
     1621 gccggtcacc tctgcctccc tcgcccatgg ctgcgacgct gcggctcacg tttcccttcg
     1681 tcgtgacgcc ctatggcatc gccgggttga tcgcggtgct cgatctggcg gcagacggca
     1741 aaacgaccgc cagcgtgtac gccctcctga tcgtggtcat gctgttgaac ctgctagcga
     1801 tgctcttcgt ccgcgagatc acgcgcggcc cggtactgct catactgcag gtgctcgggg
     1861 cggtgctcgg cgtactgcag ttcgccctcg ccatacagat catcatccgc gggttgcgcg
     1921 atctgaacgt tctgcccgcc taggaccttg ggcccatggc cgcatgagcg cggtgtgagg
     1981 agaatttgct cgaaatccgg gacgcccgat gaccgacgcc ctcgaatcga aagtgcgcat
     2041 gcccgcccag ccgcaggatc ccgcgcaggc tgcagagccg gcccacccgg agatggtctg
     2101 gatcgcgggt
//
LOCUS       DATDMP010000221         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1033966, whole genome shotgun sequence.
ACCESSION   DATDMP010000221 DATDMP010000000
VERSION     DATDMP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033966"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="ftcD"
                     /locus_tag="VI195_03760"
     CDS             complement(<1..1070)
                     /gene="ftcD"
                     /locus_tag="VI195_03760"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY6823529.1"
                     /translation="MRVAKLASYSFADMSTLVECVPNFSEGRDKAKVDAIVAAMKMDG
                     VHLLDCETDADHNRCVITLVGERAPIQEAVIRGVGKAAELIDLNVHQGAHPRMGAADV
                     VPFVPIEGVSIEDCVAMARQAGAEIWRRYQIPVYLYEAAATAPERQNLETIRRGQFEG
                     IRAEIATSSARKPDFGDPRVHPTAGVTAVGARKFLIAYNVFLNTPDVQIAKNIAKAIR
                     FSSGGLRFVKGAGFLVRGMAQISMNLTDFEQTPVHRVFEMVKREAARYGVAPVSSEIV
                     GLIPKRALEAAAEWFLQLEKFDSSSILENRLRAVMGGKTAVGGLRAGVEPFVEQLAAP
                     TATPGGGSAAAASAAMAAALAT"
     gene            complement(1118..1771)
                     /locus_tag="VI195_03765"
     CDS             complement(1118..1771)
                     /locus_tag="VI195_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6823530.1"
                     /translation="MKPYLRFVAALALLAGPAAPALAQTYQYSYPAVRPVQWYIDGGA
                     SITEGQTAGDFDNGFTIGTGVTVHPSPGPFALRLDLNYSRSAATDQFIALNEAATGTP
                     IDHGSMQTVTGFVNAVFEAPINPWARFYATGGVGLGYRRIELTQNGFFCDPFFCGPGF
                     SNALVASSDVTRFAWNAGGGVDFMLPGGQSWFIEARYERLETEAPTEFIPIRFGFRF"
     gene            complement(1837..>2492)
                     /locus_tag="VI195_03770"
     CDS             complement(1837..>2492)
                     /locus_tag="VI195_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY6823531.1"
                     /translation="ELARGMRLAFLMTIPASIGLMVLAEPIISVLYQHGRFGAHETAE
                     SAGALRYYAIGLCGYAALKVLVNAFYAIDRRKTPMVVSFIAVGLNLLLNWIFTVQLGW
                     GHRGLAFSTACVATSNFLILYLLMRTHLGRLESRAMGVLLAKVALASLALLAIAWGGG
                     QLLLAGWASERFWPKLASLLLVIACAAAAFFACASALRIGEVRDLAGALRRRLQRAR"
BASE COUNT          443 a          846 c          816 g          387 t
ORIGIN      
        1 atcgtggcca atgccgcggc catggccgca ctggcggccg cggcgctgcc gcccccgggt
       61 gtcgcagtcg gcgcggcaag ctgctcgaca aacggctcga ctccggcgcg caagccgccc
      121 accgccgttt tgccgcccat caccgcacgc aggcggtttt ccagaattga tgacgaatcg
      181 aacttctcga gctgcagaaa ccattccgct gccgcttcca gggctcgttt cgggatcaat
      241 ccgacgatct cgctgctgac gggcgccacg ccgtagcgcg ccgcttcgcg cttcaccatc
      301 tcgaacaccc gatggacggg cgtctgctca aaatcggtga ggttcatgga aatctgggcc
      361 atgccacgca ccagaaatcc tgcgcccttt acgaaccgca gcccgccgct ggaaaagcga
      421 attgccttgg caatgttctt cgcgatctgc acatccggcg tgttgagaaa aacgttgtag
      481 gcgatgagaa atttccgcgc gccgaccgcc gtgaccccgg ccgtcggatg tactcttgga
      541 tcgccgaagt ctggcttcct tgcggaactg gtcgcgatct cggcccgaat gccttcgaac
      601 tgcccgcggc ggatggtttc caggttctgc cgctcggggg ctgtggcggc ggcctcgtac
      661 agatacaccg gaatctgata acgccgccag atctcggcgc cagcttgccg cgccatggcc
      721 acgcagtctt cgatgctcac accctcgatg ggaacgaacg gcaccacgtc cgctgcgccc
      781 atgcgcggat gcgctccctg gtgaacgttc agatcgatca gctcggccgc cttgcccacg
      841 ccgcggatca ccgcttcctg aatcggcgcg cgctcgccga ccagcgtgat tacgcagcgg
      901 ttatggtcgg cgtccgtctc gcaatcgagc agatgcacgc cgtccatctt catggccgca
      961 acgatggcgt ccaccttcgc tttgtcgcgg ccctcggaga aattgggtac acactcgacc
     1021 agcgtcgaca tatcggcaaa agaatagctg gcgagcttgg caactcgcaa gccagctgcc
     1081 cggaaaggca atgaggcgcg accgcgcgct cgcggcgcta gaagcggaaa ccgaagcgga
     1141 tcgggatgaa ttccgtcggc gcctcggtct cgaggcgctc gtagcgcgcc tcgatgaacc
     1201 aggactgtcc gccggggagc atgaagtcga cgcccccgcc cgcattccag gcgaagcgcg
     1261 tcacgtcgct gctcgccacc agggcgttgc tgaagccggg tccgcagaag aacgggtcgc
     1321 agaagaagcc gttctgcgtg agctcgatgc gccggtaccc gagccccacc ccgccggtgg
     1381 cgtagaaacg cgcccagggg ttgatgggcg cctcgaatac cgcgttgacg aacccggtga
     1441 cggtctgcat cgagccgtgg tcgatcggcg taccggtcgc ggcctcgttc agcgcgatga
     1501 actgatcggt cgccgcggag cggctgtagt tcagatccag ccgcagcgcg aacgggccgg
     1561 gactgggatg caccgtcact ccggtgccga tggtgaagcc gttgtcgaaa tccccggcgg
     1621 tctgcccctc ggtgatgctc gcgccaccgt cgatgtacca ctgcaccggc cgcaccgccg
     1681 gataggaata ctggtacgtc tgtgcgagcg ccggagccgc aggaccggcc agcagggcga
     1741 gcgccgcaac gaatcgcagg tatggcttca tggattcatc gtcccgtgta gtggctgaat
     1801 cgcgcctgaa cgccgcggcg tgcggcagcg cggcgctcag cgcgcgcgct gcagccggcg
     1861 ccgcagcgcg cccgcgagat cccgcacttc gccgatccgc agcgcactcg cacaggcgaa
     1921 gaacgcggca gccgcgcagg cgatgacaag cagcagactc gccagcttcg gccagaagcg
     1981 ctcgctcgcc cagcccgcga gcagcagctg cccgccgccc caggcgatgg cgagcagcgc
     2041 cagcgaggcg agcgccacct tggcgagcag cacgcccatg gcgcgcgact cgagccggcc
     2101 gaggtgcgtg cgcatcagca ggtagaggat gaggaaattg ctggtggcga cgcaggcggt
     2161 ggaaaacgcg agtccccggt gaccccagcc gagctgcacg gtgaagatcc agttgagcag
     2221 caggttgagc cccacggcga tgaagctcac caccatcggg gtcttgcgcc ggtcgatcgc
     2281 gtagaaagcg ttcaccagca ccttcagggc ggcgtagccg cacaggccga tggcgtagta
     2341 gcgcagcgcg ccggccgact ccgccgtctc gtgcgcgccg aagcggccgt gctggtagag
     2401 caccgagatg atcggctcgg cgagcaccat cagcccgatg ctggccggta tggtcatgag
     2461 gaacgcgagc cgcatgccgc gggcgagctc gc
//
LOCUS       DATDMP010000222         3510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1811917, whole genome shotgun sequence.
ACCESSION   DATDMP010000222 DATDMP010000000
VERSION     DATDMP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811917"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /gene="fahA"
                     /locus_tag="VI195_03775"
     CDS             <1..1373
                     /gene="fahA"
                     /locus_tag="VI195_03775"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEY6823532.1"
                     /translation="GGCPRCPPAREGAQAIAPVTGPDATHDPALRSWVDSANAAGTDF
                     PLQNLPFAAFRRAASTEGFRGGVAIGDSVLDLGALHALGVFTGPAAEALALCRGPALN
                     PFMAAGREASAALRAALSGALRSGSPLAPRLRALLIPQSAAAYRVAADVGDFTDFYAS
                     IHHATAVGRLFRPDNPLLPNYKWMPIAYHGRASSVLVSGESFARPHGQIMPAGAKAPE
                     LAATRRLDYEVEVGVFVGRANQLGERIPLAEAERHLFGLCLLNDWSARDVQAWEYQPL
                     GPFLAKNFATTVSPWVVTLEALAPFRVPWTRPAEEAAPLGYLDDAAQRRAGAIDLQLE
                     AWLDTARMRAQGLPAQRLSQSSFRDSWWTIAQMLTHHTVNGCNLAPGDLLGSGTQSGP
                     APSAAGSLLELTAGGRQPLALGDGETRTFLEDGDRVVLRGWCERPPYVRIGFGEAAAT
                     VLPARP"
     gene            1370..2101
                     /locus_tag="VI195_03780"
     CDS             1370..2101
                     /locus_tag="VI195_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY6823533.1"
                     /translation="MSTAPWPVAAERGARSYRYYDLILGAYVCVVLCANLIGAAKVAT
                     VHVPGWGPLTFMAGVLFFPISYFFGDILTEVYGYARDRRVVWSGFAALTFASVMSVVI
                     VRLPPADFWRPHQSAVEAMFGNTPRIACGSIVAFWCGSFVNSYVLAKMKLLTRGRWLW
                     TRTIGSTLCGELIDSALFYTIAFAGLWPTADLIAVTLTQYVLKSAWEVLATPATYWIV
                     GALKRAEQEDYYDRDTNFTPFSLRA"
     gene            complement(2192..2533)
                     /locus_tag="VI195_03785"
     CDS             complement(2192..2533)
                     /locus_tag="VI195_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823534.1"
                     /translation="MLKSIRSAVTAAAAAVALAGLASNALADETQWQKDHPRRTEVNA
                     RLANQNKRINKELKEGEINKAQAHKLHAEDHAIRQEERTMASTNGGHITKAEQRSLNQ
                     QENQVSKQIGK"
     gene            3125..>3510
                     /locus_tag="VI195_03790"
     CDS             3125..>3510
                     /locus_tag="VI195_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823535.1"
                     /translation="MRSFGAALLGVAVAAAPALTYAQHSGGGGGGGGGHASGGGGHFG
                     GGGHFGGGGHVLGGSPHFGGFAGGHSLGSYGGGHTFAAPHYGVSGHSFSYGTRYFAPS
                     SHGVTYSGAHYARSGYVSRPGYVGGRP"
BASE COUNT          486 a         1172 c         1199 g          653 t
ORIGIN      
        1 ccggcggctg cccgcggtgc ccgcccgccc gcgaaggcgc gcaagcgatc gcgccggtga
       61 ctgggcccga cgccacgcac gacccggcgc tgcgcagctg ggtcgacagt gccaacgcgg
      121 ccggcacgga tttcccgctg cagaatctgc cgttcgccgc gttccggcgc gccgcgagca
      181 cggagggatt tcgcggcggg gtggcgatcg gcgactcggt gctcgacctc ggcgcactgc
      241 acgcgctcgg agtatttacc gggcctgccg ccgaggcgct ggcgctgtgc cgcgggccgg
      301 cgctcaaccc attcatggca gcaggccgcg aggccagcgc ggcgctgcgt gccgcgctct
      361 cgggcgcact gcgtagcggc tcgccgctcg cgccgcgcct gcgcgcgctg ctcatcccgc
      421 agtccgccgc cgcataccgt gtcgcggccg acgtcggaga tttcaccgac ttctatgcct
      481 cgatccatca tgccacggcc gtcggacggc tgttccgccc ggataatccg ctgctgccca
      541 actacaagtg gatgccgatc gcctatcacg gccgcgcctc ctcagtgttg gtttccggtg
      601 agtcgtttgc gcgaccgcac ggccagatca tgcccgcggg cgccaaggct ccggagctcg
      661 ccgccacgcg ccgcctcgac tacgaggtgg aggtcggagt gttcgtcggt cgcgccaatc
      721 agctcggtga gcgcatcccg ctcgcggaag ccgagcggca cctcttcggg ctgtgcctgt
      781 tgaatgactg gtcggcgcgc gatgtgcagg cctgggagta ccagccgctc ggaccgtttc
      841 ttgccaagaa cttcgccacc accgtgtcgc cgtgggtggt gacgctggag gcgctcgcgc
      901 cgtttcgcgt gccgtggacg cggccggcgg aggaggccgc accgctcggc tacctggacg
      961 acgccgcgca gcgccgcgcg ggcgcgatcg atctgcaact ggaggcctgg ctcgataccg
     1021 cgcgcatgcg cgcccagggg ctgcccgcgc aacgcctgtc acagagctcc ttccgcgact
     1081 cgtggtggac catcgcgcag atgctcaccc accacaccgt gaacggctgc aacctcgcgc
     1141 ccggtgatct gctcggcagc ggcacccagt ccggcccggc gccgtccgcg gccggatcgc
     1201 tcctcgagct caccgcgggc gggcgacagc cgctcgcgct gggtgatggc gagacgcgta
     1261 cttttctcga ggacggtgac cgggtggtgc tgcgcggctg gtgcgagcgg ccgccttatg
     1321 tgcgcatcgg cttcggcgag gccgcagcga ccgtgctgcc ggcgcggccg tgagcaccgc
     1381 accctggccg gtcgccgccg agcgcggcgc gcgcagctac cgctactacg atctcatcct
     1441 gggtgcgtac gtgtgcgtgg tgctgtgtgc caatctcatc ggcgcggcca aggtggcgac
     1501 cgtgcacgtg ccgggatggg gtccgctcac cttcatggcg ggcgtactgt tctttcccat
     1561 ctcctacttc ttcggcgaca tcctcaccga ggtgtacggc tatgcacgcg accggcgcgt
     1621 ggtgtggagc ggcttcgccg cgctcacatt cgcttcggtc atgtcggtgg tgatcgtgcg
     1681 gctgccgccc gcggatttct ggcggccgca ccagagcgcc gtcgaggcga tgttcggcaa
     1741 cacaccgcgc atcgcctgcg gctcgatagt ggcgttctgg tgcggctcgt tcgtcaacag
     1801 ctatgtgctc gcgaagatga agctgctgac gcgcgggcgc tggttgtgga cacgcaccat
     1861 cggctcgacg ctctgtggcg agctcatcga ttccgcgctc ttctacacca tcgcctttgc
     1921 gggactgtgg ccgaccgcgg atctcatcgc ggtgacactg acacagtacg tgctgaagtc
     1981 ggcgtgggaa gtgctcgcca cgcccgccac ctactggatc gtcggcgcgc tcaagcgcgc
     2041 ggagcaagag gactactacg atcgcgacac taacttcacg ccgttctcgc tgcgcgcctg
     2101 aagccgtggc gcgccgccat ctgggaattc gcggcgcaaa aaagggcggg agcgcagtgc
     2161 gcggcgctcc cgcccttcag ctgcaaaccg cttacttgcc gatctgcttg ctgacctggt
     2221 tttcctgttg gttgagcgag cgctgctcgg ccttggtgat gtggccgccg ttcgtgctcg
     2281 ccatggtgcg ctcttcctga cggatggcgt gatcctcggc gtgcagcttg tgcgcctgcg
     2341 ccttgttaat ctcgccttcc ttcagctcct tgttgatgcg cttgttctga ttcgcgaggc
     2401 gggcattcac ctcggtgcgg cgtggatggt ccttctgcca ttgcgtctca tcagccagcg
     2461 cattgctcgc aaggcccgcg agcgctacgg cggcggccgc ggcggtcacg gcgctacgaa
     2521 tggatttcaa catgagtcgg tcctcctgaa agactgtgtc gagtgttcgc tattgagccg
     2581 gaacttccgg ctacagggcg ttaacaacgt cgcagcttgt ggagtgctga cctttgtgac
     2641 ggaaagttaa ccaacagcgc gagtgtgaac tgagtcacgt tcgcaaagcg cagcacacat
     2701 gttggtgttg ggcaggccct ggctgcttcg cggtgcaagc acggctgcgc tctttgagtg
     2761 ttggcacgag cagacgcatg caggtgctac cggcgatcgc cctcaacacg gtgttctgac
     2821 gcgcatgggc gctctgcggg aacggccggc gttcggcggc tgaacccgag gtgaatggct
     2881 cccgtgcagc cggccgcgcc ttgaaaggcc gggcgtgacc cccatgcccg ttataccggg
     2941 aggcggttat gacagtggtc aacgaccccc ggcgtgactg cgttttaatg acagtgagct
     3001 tgaacagcag cggcggctgg gcgtctaatg aattgccagc cagcgccctg gaaatgagct
     3061 cggatctgaa gcggggtgat gagctgtgag taagccgatg atcaaagcga ccgcgcgata
     3121 tgtaatgcga agcttcggcg ccgcactgct tggagtggcg gtcgcggctg ccccggctct
     3181 gacctatgcc cagcatagcg gcggtggcgg cggcggcggc ggcggccatg cctcaggggg
     3241 cggtggccat ttcggcggcg gcgggcactt cggaggcggg ggccatgtcc tcggcggtag
     3301 cccccatttc ggcggttttg cgggcggcca tagcctcggt tcctatggcg ggggccacac
     3361 cttcgcagcc ccgcactatg gtgtttcggg ccactccttc tcctacggca cgcgctattt
     3421 cgccccctcg agccatggcg ttacctatag cggggctcat tacgcccgct caggctacgt
     3481 ctcgcgtcca gggtacgtag gggggcggcc
//
LOCUS       DATDMP010000223         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1589600, whole genome shotgun sequence.
ACCESSION   DATDMP010000223 DATDMP010000000
VERSION     DATDMP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589600"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VI195_03795"
     CDS             complement(<1..964)
                     /locus_tag="VI195_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6823536.1"
                     /translation="MTKFPMKVGIVGSGGISGHHLRAISNYGNCQIVAIVDRDLTRAR
                     AQAERFNVPHTFESLAPLLALKPDIVHVLTPPDSHTPLVIEALAGGAHVYVEKPMAIA
                     AADCEAMINAATRANRQLCVGHCWNYIPVIARAQRLIASGTAGEVLQVAASFNYDVMR
                     NPTFGTGHWSSKLPGGLIEDLAVHLISVLIRLLGAPQQASALSRSSGQVPGTPVDEMR
                     AIIEGERGLGTLSVSLRGRPDLILIDIWCTRMLLRLNVSSMSLAIHRQLPVPQKIARG
                     LANLDVAAQLVGGTIDATWKLARGKVDGSYGIAPCIHAFYDALAS"
     gene            complement(1034..1444)
                     /locus_tag="VI195_03800"
     CDS             complement(1034..1444)
                     /locus_tag="VI195_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823537.1"
                     /translation="MARNYVTLAAAAVDQSGHINYVLIGYIWSVGAAPDPKDARDAAE
                     GLTLQADERRVELTLQQRSAREFGIGVAVHRPPVGSVTPYIYVTDLATVQQLAESSHL
                     SLSIQKQNAAADYQLFTDGRSALREFVRFAQARF"
     gene            complement(1879..2796)
                     /locus_tag="VI195_03805"
     CDS             complement(1879..2796)
                     /locus_tag="VI195_03805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6823538.1"
                     /translation="MPTAHGLAALREMLQASYFPSWFAGAEMEQFLASPEGHEAFRNH
                     LFYRLEMDRYELVPWINSVLPLRGTRILEIGCGTGSGTVALAEQGAQVTALDVHAEAL
                     QVAEKRCEIHGVGGVRFVQANAERLTSMYRTGQFDLVLFFAVLEHMTLSERDMALRSA
                     WTLLAEGQYLGLGETPNRLWPYDTHTSRLPFFNWLPDELAFRYSQFSPRLSLRTRFRE
                     MTEESMLRFRREGRGVSFHELDLALGESYQVISDQTAYLAARNPLKRFKRILARDGAR
                     ERALNAYSPGRHRALFRQSLDLLIRKSAA"
BASE COUNT          615 a         1008 c         1011 g          560 t
ORIGIN      
        1 cgctcgcgag cgcgtcgtag aaagcatgta tgcacggcgc gataccatac gacccgtcca
       61 ctttcccgcg cgcgagcttc caggtcgcat cgatcgttcc gcccaccagt tgtgcggcga
      121 catcgagatt ggcgagtccg cgcgcgatct tttgcggcac cggcagctgt cggtggatcg
      181 cgagtgacat cgaagagacg ttgagtcgca gcagcatgcg cgtacaccag atgtcgatca
      241 ggatgaggtc ggggcggccg cgcagtgaga cggagagcgt gccgagtcca cgctcgcctt
      301 cgatgatggc acgcatctcg tccaccggcg tacccggcac ctgaccgctc gaacggctga
      361 gcgcgctcgc ctgctggggc gcgcccagca ggcgaatcag taccgagatc aggtggaccg
      421 cgaggtcctc gatgagcccg cccggtagct tgctcgacca gtgcccggta ccgaaggtcg
      481 ggttgcgcat cacgtcgtaa ttgaacgagg cggcgacctg caatacttca ccagcggtcc
      541 cggaggcgat cagccgttgc gcccgcgcga taaccggtat gtagttccaa caatgcccca
      601 cgcacagctg ccggttggca cgggttgcgg cattgatcat tgcctcacaa tcggcggccg
      661 caattgccat gggcttttcc acgtatacgt gagccccacc cgccagcgct tcgattacca
      721 gcggagtatg ggaatcgggc ggcgtcaaca cgtgcacgat gtccggtttg agcgccagca
      781 agggcgcgag cgactcgaac gtgtggggca cgttgaagcg ctcggcttgc gcgcgcgcac
      841 gtgtcaggtc gcgatcgacg atggcaacga tttgacaatt gccatagttc gagatcgcgc
      901 gcagatgatg cccggagatc ccaccgcttc cgacgatgcc gactttcata gggaactttg
      961 tcattggctg ggcagtgagt ctaacatcga ttcgcagcgc gtcggcgggc cgcaccgcgc
     1021 gtcgcagggt tgctcagaac ctggcttgcg cgaagcgcac gaattccctg agcgcgctgc
     1081 gcccatccgt gaacagctgg taatccgcgg cagcgttctg cttctggatg gaaagcgaca
     1141 gatggctcga ctcggccagc tgttgcacgg tggcgagatc ggtcacatag atgtagggcg
     1201 tcacggaacc caccggtgga cggtgcacag ctaccccgat accgaactca cgggccgaac
     1261 gctgctgcag cgtcagctcg actcgccgct catcggcctg cagcgtgagt ccttccgctg
     1321 catcgcgcgc gtccttggga tcgggcgcag caccgaccga ccagatgtag ccgatgagta
     1381 cataattgat atggccgctc tgatccaccg ccgccgccgc cagggtaacg tagttgcgcg
     1441 ccaaggctga gcgctcctga gcgaagacca ggggctcgcg gacgaccgcc acggtcgcac
     1501 ccgtggactc atcgagatac tcgctgggcc cgcggctcga ggcgacggcc agggccgccg
     1561 cgagcaggca ggccgtcgcg caagcgtgcc cgcgaagtcg aaagcgtgaa cgggtaatca
     1621 cgttggcaat gatgtggagg tgccgggaat gatcaggcac cgcgcgtcgc ggcgttgtta
     1681 ttggtggcgc tggcgccgcg taaccatccc ggcaggtgat aaatgcacag cccatgccag
     1741 aaccgccaag cctttgatct gcgacaaggg cgcgcgcacc gccagcggcg ctgttcgtcg
     1801 agaatgtaaa ctccttcgac ggaatcaaac agcaaccggg cccagcgcat ggcgcccaac
     1861 tcgttagcat ccagttgctc acgcagcaga cttccgtatc agcagatcca ggctctgcct
     1921 gaacaacgcc cggtgccgac ccggggaata agcattgagg gcgcgctcgc gtgctccatc
     1981 acgcgccagg atccgcttga atcgtttgag tggattgcgc gctgccaggt acgcagtctg
     2041 atcgctgatc acctggtagc tctcgccgag tgccagatcg agctcgtgaa agctgacgcc
     2101 acggccctca cggcgaaagc gcagcatcga ctcctcggtc atttcgcgga atcgagtgcg
     2161 cagcgagagc ctcgggctga attgcgaata gcggaacgcc agctcatcgg gcagccagtt
     2221 gaagaacggc aagcgcgagg tatgggtgtc atagggccac aggcggttcg gcgtctctcc
     2281 gagacccagg tattggccct cggcaagcag tgtccaggcc gagcgtaatg ccatatcgcg
     2341 ctcgctgaga gtcatgtgct ccagtacagc aaaaaacagc accagatcga attgtccggt
     2401 gcgatacatg ctggtgaggc gttcggcgtt tgcctgaacg aaacggacgc cgccgacacc
     2461 atgtatctca caacgcttct cggcgacctg aagagcctcg gcatgcacat cgagtgcggt
     2521 cacctgcgca ccttgctcag ccagcgcgac cgtcccggag ccggtaccgc agccgatttc
     2581 cagaatgcgc gtaccccgga gcggcagcac gctgtttatc catgggacca gctcgtagcg
     2641 atccatctcc aggcggtaga agagatgatt gcgaaaggcc tcgtgcccct cgggcgaggc
     2701 cagaaactgc tccatctccg ctccggcgaa ccagctcgga aaatagctcg cctgcagcat
     2761 ctcgcgcagc gccgcgaggc cgtgcgctgt gggcacggtg aaatttcttc tcagccggcg
     2821 tgtgaccgca agattcccgt gtcgggatag gaaacgcaat ctctgccgca gcgcggagtt
     2881 ctgggggatt attctgtcta gcaccgtttc tgccgccggt ggtcgatgcg cctcgagcat
     2941 ctacccgctt gccttgccgt gccgcatcct cgcacgcgca gcaagcacga taccagcgga
     3001 tcttcgcaca gcagcgcgct agcgcacgag cgtcaccccg tgcagctagc gcccgggggc
     3061 gctcacccta gtccagcgtg gcggacagtt ccggtgagaa tccgctttcc gctcccgcgg
     3121 agttgtagga ggttacggtg aagaaatagc ggccggttgg caggttctgc accacgtacg
     3181 aggtcagtcc agga
//
LOCUS       DATDMP010000224         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_145478, whole genome shotgun sequence.
ACCESSION   DATDMP010000224 DATDMP010000000
VERSION     DATDMP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145478"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1437
                     /gene="lon"
                     /locus_tag="VI195_03810"
                     /pseudo
     CDS             1..1437
                     /gene="lon"
                     /locus_tag="VI195_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            1705..1780
                     /locus_tag="VI195_03815"
     tRNA            1705..1780
                     /locus_tag="VI195_03815"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1738..1740,aa:Val,seq:tac)
     gene            complement(1867..>2069)
                     /locus_tag="VI195_03820"
     CDS             complement(1867..>2069)
                     /locus_tag="VI195_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823539.1"
                     /translation="PDTFIATETNRRLSLALRQEAARTTQLTDSRRGLTRWLVALTVL
                     LVGLTLLLVGLTVALIWIELRR"
BASE COUNT          436 a          635 c          670 g          328 t
ORIGIN      
        1 aaggtgccgt ggaagaaacg caccaagatc ctcaaggaca tcgcccgcgc cgagcaggtg
       61 ctcgaggaag atcactatgg gctggagaag gtcaaggagc gcatcgtcga atacctggcc
      121 gtccagcagc gcgtcgacaa gatcaagggc cccatcctct gcctcgtggg accgccgggc
      181 gtcggcaaaa cctcgctcgg gcagagcatc gcgcgcgcca cgaaccgcaa gttcgtgcgc
      241 atgtcgctcg gcggcgtgcg cgatgaggcg gagatacgcg gacatcggcg cacctacatc
      301 ggctcgatgc cgggcaagat cgtgcagaac atgtccaagg ccggcgtgca caacccgctg
      361 ttcctgctcg atgaggtcga caagatgtcg atggacttcc gcggcgaccc ctcctcggcg
      421 ctgctcgaag tgctcgatcc ggagcagaac tcgaccttca acgatcatta cctcgaggtc
      481 gacctcgatc tctccgaggt catgttcgtg tgcacggcga acagcctcaa cattccgccc
      541 ccgctgctcg atcgcatgga agtcatccgt ctaccggggt acaccgaaga cgaaaagatg
      601 aatattgcgc ggcgctactt ggtgccgaag cagatgaaga gcaacgggct gaagcccgat
      661 gagttgaagg tgtcggactc ggcgctgctc gacatcgtgc gctactacac ccgcgaagcc
      721 ggggtgcgca atctcgaacg cgagatcgcc aagatcggcc gcaaggcggt caagcagatg
      781 ctgctgcaca agaaggagca gaagtcggtc tccgtcactc cgcgtaacct cgacaagttt
      841 ctcggcgtgc gccgcttccg ctacggcaag gccgaggaca tgaaccgcat cggccaggtg
      901 accgggctcg cttggacgga ggtgggcggt gagctgctca ccatcgaggc ggcggtcgtt
      961 cccggcaagg gcaagctgca gcacaccggg cagctcggtg aagtcatgca ggagtcgatc
     1021 caggcggcga tgaccgtggt gcgcagccgc gcgcaggtgt tgggactcga gccggagttc
     1081 taccagaagc acgacgtgca cctgcacatt ccggaaggcg caacgcccaa ggacggcccg
     1141 agtgccggta tcggcatgtg cacggcgctg gtttccgcgc tcaccaagat cccggtgcgt
     1201 tcggatgtgg cgatgacggg cgagatcacg ttgcgcggtg aagtcctgcc gatcggtggt
     1261 ctcaaggaga aactcctggc ggcgcatcgc ggcggcatca agacggtgct catcccgaat
     1321 gaaaacgaga aggacctggc ggagattccc gacaacatca agggcaatct cgacatccgc
     1381 gcggtgaagt ggatcgatga ggtgctcggc gtcgcactca cccagcagcc catgccggga
     1441 cagcaggcgg atgcaccagc cacgcccacg gcgggcgctg aggactccga ggagaagcgt
     1501 gcgcgccgca atggccggcg agtgaagcag gtgccgccgc cggctcactg agcggggccg
     1561 atgcccgctg ccgaacagcg cgcagcgcat gcagttaaca gacgtttcgg caatagttaa
     1621 ccaaatggtt aaccattatc aaagggtctg caggtgcgcg ccgcctttga gggcaacgag
     1681 gctggtggcc actaattaca accagggtgc ttagctcagc tggtagagcg tcgcccttac
     1741 aaggcgaatg tcggcggttc gatcccgtca gcacccacca aaaatcaatc acttacggat
     1801 taaccccttc tgatagctct tccgtaagcg catggtaagc gccggaagga taggtaagcg
     1861 ccgcctctac cgccgcagct cgatccagat gagcgccacg gtgaggccca ccagcagcag
     1921 ggtgaggccc accagcagca cggtgagagc taccagccat cgagtgagcc cccgcctgga
     1981 atcggtaagc tgcgtcgtcc gcgccgcttc ctggcgcaac gccagtgaca gtcgccggtt
     2041 ggtctcggtg gcgatgaacg tgtcgggcg
//
LOCUS       DATDMP010000225         3078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_812777, whole genome shotgun sequence.
ACCESSION   DATDMP010000225 DATDMP010000000
VERSION     DATDMP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812777"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /gene="uvrB"
                     /locus_tag="VI195_03825"
     CDS             complement(<1..1952)
                     /gene="uvrB"
                     /locus_tag="VI195_03825"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY6823540.1"
                     /translation="MEQDNRPFKLEANFAPAGDQPQAIEQLVEGINEGLAHQTLLGVT
                     GSGKTFTAAAVIERIQRPTMVLAHNKTLAAQLYGEFREFFPHNSVEYFVSYYDYYQPE
                     AYVPSSDTYIEKDASRNEHIEQMRLSATKALLERRDSIIVATVSSIYGLGDPEAYLAM
                     VLHLKRGDRLDQRALLRRLAEMQYTRNELDLTQGNYRVRGDVIDIFPAESEREAVRVE
                     LFDDSIDSITLFDPLTGAISRKVPRFTIYPGTHYVTPRERLIGAVDQIREDLRVRLKE
                     LRDAGRLVEAQRLEQRTRFDMEMIKEVGYCAGIENYSRYLSGRAPGEPPPCLFDYLPD
                     DALLIVDESHQTIPQLGAMYRGDRSRKEVLVEYGFRLPSALDNRPLKFEEWERLAPQM
                     IFASATPGDYEAKHSAQVVEQVVRPTGLVDPQVEVRPVGTQVDDVLSEIRKTSACGER
                     VLITTLTKRMAEDLTDYLGEHDVRVRYLHADIETVERTEIIRDLRLGKFDVLVGINLL
                     REGLDMPEVSLVAVLDADKEGFLRSDNSLIQTIGRAARNIHGRAIFYADRMTGSMQRA
                     IDETNRRRRKQLDYNSAHGITPRGIEKAVLEIMEGARPEAGVLPLPRRRGEKPVSPEE
                     LKPEALLRQIKKLEAEMFRRARNLEF"
     gene            2063..>3078
                     /locus_tag="VI195_03830"
     CDS             2063..>3078
                     /locus_tag="VI195_03830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6823541.1"
                     /translation="MTLPVSRRVQRVKPSPTLAVSARAARLRAQGKDVISLGAGEPDF
                     DTPEHIAQAAIDAIRAGFTRYTNVDGIDELKDAIIAKFERDNALSYERPQILVSAGAK
                     QTIYNLCMAVLDPGDEAVIPAPYWVSYPDMVLLADGLPVMPFAGMAQAYKITPRQLAV
                     AITAKTRLVLLNSPCNPTGAAYTRAELRALGDVLLEHPRIVIGTDDMYEKIYWGPEPF
                     CSLLTAVPELYSRTVTINGVSKAYAMTGWRIGYCGGPRELIAAMATIQGQSTTNASSI
                     SQKAAAAALSSDQRCIATMNAAYKERRDFLVRALNALPGISCLPGDGTFYAFADVSRA
                     MAALG"
BASE COUNT          531 a          984 c          975 g          588 t
ORIGIN      
        1 tcaaattcga gattgcgtgc gcggcggaac atctccgcct cgagtttctt gatctgcctg
       61 agcagcgcct cgggcttcag ctcctcaggg ctcacgggct tctcgccacg gcggcgcggg
      121 aggggcagta cacccgcttc cggccgcgcg ccctccatga tctccaacac tgccttttcg
      181 atgccgcgcg gcgtgatgcc gtgtgcggag ttgtagtcga gctgctttct gcggcggcgg
      241 ttggtttcat cgatggcccg ctgcatggag ccggtcatgc ggtcggcgta gaagattgcc
      301 cgtccgtgaa tattgcgcgc cgcacggccg atggtctgaa tcagggagtt gtccgagcgc
      361 aggaaccctt ccttgtcggc atcgagaacg gcaaccagcg agacctccgg catgtcgagc
      421 ccttcgcgca gcagattgat gccgaccagc acatcgaatt tgccgaggcg cagatcgcgg
      481 atgatctcgg tgcgctcgac ggtctcgatg tcggcgtgca ggtaacgcac gcgcacatcg
      541 tgctcaccga gataatcggt cagatcttct gccatgcgct tggtgagcgt cgtgatgagc
      601 acccgctcac cgcaggcgct ggtcttgcga atctcggaaa gcacatcgtc cacttgcgta
      661 ccgaccggcc gcacctcgac ctgcgggtcc acgaggccgg tcggacgcac gacctgctcg
      721 accacctgcg cggagtgctt ggcctcgtag tccccggggg tcgcggacgc gaagatcatc
      781 tgcggcgcga gccgctccca ttcctcgaac ttcagcggcc ggttgtcgag tgcggatggc
      841 aggcggaagc cgtactcgac cagcacctcc ttgcgcgagc ggtcgccgcg gtacatggcg
      901 ccgagctgtg gaatcgtctg gtggctttca tcgacgatca ggagtgcatc gtcgggcaga
      961 tagtcgaaaa ggcacggtgg cggctcgccc ggggcacgcc cggagagata gcgcgagtag
     1021 ttctctatgc cggcgcagta gcccacttcc ttgatcattt ccatgtcgaa tcgcgtgcgc
     1081 tgctcgagac gctgcgcttc caccagacgc ccggcgtcgc gcagctcctt gaggcgcaca
     1141 cgcagatctt cacgaatctg gtcgaccgcg ccgatgaggc gctcgcgcgg tgtcacgtag
     1201 tgcgtgcccg ggtaaatggt gaagcgcggg actttgcgcg agatcgcccc ggtgagcgga
     1261 tcaaagagcg taatcgagtc gatgctgtca tcgaacagct cgacccgcac tgcctcgcgc
     1321 tcggattcgg ctggaaagat atcgatgaca tcgccgcgca cccggtagtt gccctgcgtg
     1381 agatcgagct cgttacgcgt gtattgcatt tcggccagcc gacgcagcag cgcacgctga
     1441 tcgagccgat cgccgcgctt caggtgcagc accatggcga ggtaggcctc ggggtcgcca
     1501 aggccgtaaa tcgacgacac cgttgcgaca atgatggagt ctcggcgctc gagcagcgcc
     1561 ttggtcgccg agaggcgcat ctgctcgatg tgctcgttcc tggacgcatc cttctcgatg
     1621 taggtgtcgg aggacggcac gtaggcttcg ggctggtagt agtcgtagta agagacgaag
     1681 tactccaccg agttgtgcgg gaagaactcg cgaaattctc cgtacagctg cgccgccagg
     1741 gtcttgttgt gtgcgagaac catcgtgggg cgctgaatgc gctcgatgac cgcggccgca
     1801 gtgaaggtct ttcccgatcc cgtcactccg agcagcgttt gatgcgcaag tccctcgttg
     1861 atcccctcga cgagctgctc gatggcctgg ggctgatccc cggcgggggc gaaattcgct
     1921 tcgagcttga agggtcggtt gtcctgttcc atgcgagagc gctgccgtcg gggttcagag
     1981 gggccgttcc cgatggtccg acctgccaca atcccttaat atagcggcgc gcccgaaaca
     2041 caagcgaact gcaggaaaat cagtgactct gcccgtctcg cgtcgcgtcc agcgcgtcaa
     2101 gccttccccg accctcgccg tcagcgctcg tgccgcgcgc ctgcgggcgc agggcaagga
     2161 cgtcatcagc ctcggcgccg gcgagccgga cttcgacact ccggaacaca tcgcgcaggc
     2221 ggccatcgac gccatccgcg ccgggttcac gcgttacacc aatgtcgatg ggatcgatga
     2281 gctcaaagac gccatcatcg ccaagttcga gcgcgacaac gcgctgagct acgagcgccc
     2341 gcagatcctg gtctccgccg gcgccaagca gacgatctac aacctctgta tggcggtgct
     2401 ggacccgggc gatgaagcgg tgattccggc gccgtactgg gtgtcttacc ccgatatggt
     2461 gctgctcgcg gatggacttc cggtgatgcc cttcgctggc atggctcaag cctacaagat
     2521 caccccgcgt cagctggccg tggccatcac tgcgaagacg cgcctcgtgc tgctgaatag
     2581 cccctgcaat ccgaccggag ctgcctatac gcgcgccgag ctgcgtgcac tcggagatgt
     2641 tctgctcgag catccgcgca tcgtcatcgg caccgatgac atgtacgaga agatctactg
     2701 ggggccggag cctttctgca gcctgctgac ggcggttccg gagctctact cccgcactgt
     2761 caccatcaac ggtgtgtcca aggcctatgc catgaccggc tggcgtatcg gctactgcgg
     2821 agggccgagg gagctcatcg ccgccatggc gaccatccag ggccagtcga ctaccaatgc
     2881 ttcaagcatc tcgcagaaag ctgcagccgc ggcgctctct agcgatcagc gttgcatcgc
     2941 cacaatgaac gcggcctata aggagcgccg tgatttcctg gttcgcgccc tcaatgcgct
     3001 tccggggatc agctgcctgc cgggtgacgg aaccttctat gcgttcgccg acgtctcgcg
     3061 tgccatggcc gcactcgg
//
LOCUS       DATDMP010000226         3678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1589944, whole genome shotgun sequence.
ACCESSION   DATDMP010000226 DATDMP010000000
VERSION     DATDMP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589944"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VI195_03835"
     CDS             complement(<1..724)
                     /locus_tag="VI195_03835"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY6823542.1"
                     /translation="MPPPPVYRTALRGLERLHEGKVRDIYALDAATMLIVTTDRLSAF
                     DVVLPDPIPDKGRVLNGISNFWFGRTQHLVPNHLTGRELASVIADADERAPLEGRAVI
                     VRRLQALPIEAVVRGYLIGSGWKDYQSSGQVCGIALPPGLALAAQLPQPLFTPATKAK
                     LGAHDQNIAFGEVAALIGPDLAAAMRDTALRLYGFAAEHARRRGIIIADTTFEFGVDE
                     AGRLTLIDEVLTPDSSRFWPADT"
     gene            785..1594
                     /gene="djlA"
                     /locus_tag="VI195_03840"
     CDS             785..1594
                     /gene="djlA"
                     /locus_tag="VI195_03840"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEY6823543.1"
                     /translation="MAIKWIGKVTGGLLGGLVLGPIGAALGVLLGHQFDEADAQEETP
                     PAPEELAATGERFFRASFQVMGYLAKADGRVSEREIAAARAVMQELRLAGAQVDEAIA
                     CFTAGKQPGYDYQAELRQLTALCQGRPDIARVFLEIQVRAALEGNDLQGAVRPLIGRL
                     AAALRVSALELMHIEAVLRIQRSGGFRAARAAGAAAAERELTEAYRVLETSAAASDEE
                     VAKAYRRQLQRHHPDKLTAHGLPESMLAHAKQRTQQIIEAWEIVRERRGIR"
     gene            complement(1591..2394)
                     /locus_tag="VI195_03845"
     CDS             complement(1591..2394)
                     /locus_tag="VI195_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6823544.1"
                     /translation="MRRVTGFPERIVCLTEETTETLYLLEEDWRIVGISGFTVRPPRA
                     RREKPRVSAFLSARTDRILELRPDLVLGFSDLQADIAARLVRAGLEVHVFNHRSVADI
                     LRMVRTLGGMIGCEVRARALADELEAGLAQVRARAAALARRPRVYFEEWDDPLISGIA
                     WVSELIELAGGADCFPELAREPLGRNRIIADPLEVPRRAPDIVLGSWCGKKFRPERVA
                     ARAGWHEIPAVQTGYLREIKSSIILQPGPAALTDGVRAIQQIIEEWAAP"
     gene            2431..>3678
                     /locus_tag="VI195_03850"
     CDS             2431..>3678
                     /locus_tag="VI195_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6823545.1"
                     /translation="MWLRIWAFLDRCFFGPTSARPGTLGATLRLLRYPYAVVRDLSRG
                     EINLRAMGLVYTTLLSLIPLLAFSFAILKLFGGHRDFQPIVYEFFRPVGGAAATELTN
                     RVMQFASRVSSGVVGSVGLALLAWTLIGTIKKVEDSFNFVWHVEQPRSFARRLAEYTT
                     LLIAGPLLLVGFLGLTHAALSSAPVQEVVRLPLLHRLSGTGIALAPYAMVTAFFTALY
                     MMVPNTRVQWRAALIGALVGGVLWAAIGKMFTAFVVYSTRLTIVYAGFAFVVAALLWT
                     YFGWLILLAGAQLSFYVQNPAYLRLGLQQLRLSSVELEQLALKLMYFVGRSHVSGGRL
                     WSVNRLANELGLPGIAVAQMATALERAGLVIVTEDDELVPARDIGGIGVIEILDIARN
                     QRSGHLVPRNLPVPPVDRVLASVE"
BASE COUNT          555 a         1278 c         1242 g          603 t
ORIGIN      
        1 aggtgtccgc cggccagaag cgcgaggaat ccggcgtcag cacctcgtcg atgagcgtca
       61 gccgcccggc ctcgtccacc ccgaattcga acgtggtgtc ggcgatgatg atgccgcggc
      121 ggcgcgcgtg ctcggcggcg aatccataca gccggagcgc cgtgtcacgc atcgccgcgg
      181 cgaggtcggg accgatgagc gccgcgactt cgccgaaggc gatgttctga tcgtgcgcgc
      241 cgagtttggc cttggtcgcc ggcgtgaaca gcggctgcgg cagctgcgcc gcgagcgcaa
      301 gtcccggcgg cagcgcgatg ccgcatacct gcccgctcga ctggtagtcc ttccagcccg
      361 agccgatcag gtaaccgcgt accaccgcct cgatcggcag tgcctgcaga cgccgcacga
      421 tcaccgcgcg tccctcgagc ggcgcgcgct cgtccgcgtc ggcgatcacg ctggcaagct
      481 cgcggccggt gagatgattc ggcaccaggt gttgcgtacg cccgaaccag aagttggaga
      541 tgccgttgag cacgcgtccc ttgtcgggaa taggatcggg caacacgaca tcgaacgccg
      601 acaaccgatc ggtggtcacg atcagcatcg tcgctgcatc gagggcgtag atgtcgcgca
      661 ccttgccctc gtgcaggcgc tcgagcccgc gcaatgcggt gcggtagacc gggggtggcg
      721 gcatgcactt ggtactatag cggccctttg agccccggac acgcaagcga gcccgacgcg
      781 ccacatggcc atcaaatgga tcggtaaggt caccggcgga ctgctcggcg ggctggtgct
      841 cgggccgatc ggtgccgcgc tcggcgtgct gctcggtcat cagttcgatg aggcggacgc
      901 gcaggaagag accccgccgg ctcccgagga gctggcagcg accggcgagc gcttcttccg
      961 cgcgagcttc caggtcatgg gctatctcgc caaggccgac ggacgcgtct ccgagcgtga
     1021 gatcgccgcg gcgcgcgcgg tcatgcagga gctgcgcctc gctggcgcgc aggtggacga
     1081 ggcgatcgcc tgcttcaccg ccggcaagca gcccggctac gactaccagg cagagctcag
     1141 gcagctcacc gcgctctgcc agggccggcc ggatattgcg cgcgtgttcc tcgagatcca
     1201 ggtacgtgcg gcactcgagg gcaacgacct ccagggagcc gtgcggccgc tcatcggccg
     1261 cctcgcggca gcgctccgag tctcggcatt ggagctcatg cacatcgagg cggtactgcg
     1321 catccagcgc agcggcggct ttcgtgccgc gcgcgcagcg ggcgccgcag cggccgagcg
     1381 cgagctgacg gaggcgtatc gcgtgctcga gacgagcgcc gcggcgagcg atgaggaagt
     1441 ggccaaggcc taccggcggc agctgcagcg ccatcacccc gacaagctca cggcccacgg
     1501 cttgcccgag tccatgctgg cgcacgccaa gcaacgtacg cagcagatca tcgaggcctg
     1561 ggagatcgtg cgcgagcgcc gcggcattcg ctagggtgct gcccactcct cgatgatctg
     1621 ctggatggcg cgtacgccgt cggtgagcgc ggccggtccc ggctgcagga tgatcgagga
     1681 cttgatctcc cgtaggtagc ccgtttgcac cgcggggatc tcgtgccacc ccgctcgcgc
     1741 ggcgacgcgc tccgggcgga acttcttccc gcaccatgac ccgagcacga tgtccggtgc
     1801 gcgccgcggc acctcgagcg gatcggcgat gatacggttg cgcccgagcg gctcgcgcgc
     1861 gagctccggg aagcaatccg cgccgcccgc gagctcgatg agctccgaca cccaggcgat
     1921 gccgctgatg agcggatcgt cccactcctc gaagtacacc cgcgggcgcc gtgcgagcgc
     1981 cgcagcgcgt gcgcgcacct gcgcgagtcc cgcctcgagc tcgtccgcca aagcgcgcgc
     2041 gcgcacttcg caaccgatca tgccgccgag cgtgcgcacc atgcgcagga tgtcggcaac
     2101 gctgcgatga ttgaacacgt gcacttcgag ccccgcgcgc accagccttg ctgcgatgtc
     2161 ggcctgcaga tccgagaagc cgagtaccag atccggccgc agttcgagga tgcgatcggt
     2221 gcgggcgctc aggaatgccg acacgcgcgg cttttcgcgc cgtgcgcgcg gcgggcgcac
     2281 ggtgaaaccg gagatgccga cgatccgcca gtcctcctcc agcagataaa gcgtctcggt
     2341 cgtctcctcc gtgaggcaca cgatccgctc cgggaagcct gttactcgcc tcatctcaca
     2401 tcccacccgc cggcactgta tccttaagag atgtggctgc gcatctgggc gtttctcgac
     2461 cgttgcttct tcggccccac ctcggcgcgt cccgggactc tcggcgcgac gctgcgcctg
     2521 ctgcgctatc cctatgcggt ggtgcgcgac ctgtcccgcg gggagatcaa cctgcgtgcg
     2581 atggggctcg tatacaccac gctgctctcg ctcattccgc tgctcgcctt cagcttcgcg
     2641 atcctcaagc tgttcggcgg ccaccgcgat tttcagccca tcgtgtacga gttcttccgc
     2701 ccggtcggcg gcgccgcggc caccgagctc accaaccggg tgatgcagtt cgccagccgc
     2761 gtgagcagcg gcgtggtcgg ctcggtggga ctcgcgctgc tcgcctggac gctgatcggc
     2821 accatcaaga aagtcgagga cagcttcaac ttcgtctggc acgtggagca accgcgcagc
     2881 ttcgcccggc ggctcgccga atacaccacg ctgctgatcg ccggcccgct gctgctcgtg
     2941 ggtttcctcg gcctgacgca cgcggcgctc agcagcgcac cggtgcagga ggtcgtgcgc
     3001 ctgccgctgc tgcaccggct gagcggcacc ggtatcgcgc tcgcgcctta tgccatggtg
     3061 accgccttct tcaccgcgct ctacatgatg gtgcccaaca cgcgcgtgca gtggcgtgcg
     3121 gcgctcatcg gcgcgctcgt cggcggagtg ctgtgggccg cgatcggcaa gatgttcacc
     3181 gcgttcgtgg tgtactccac gcgactcacc atcgtgtatg ccggattcgc cttcgtggtg
     3241 gcggcgctgc tgtggaccta cttcggttgg ctgattctgc tcgccggtgc acaactgtcg
     3301 ttctacgtgc agaatcccgc gtatctgcgc ctcggtctgc agcaactccg gctctcgagc
     3361 gtggagctcg agcagcttgc actgaagctc atgtacttcg tcgggcgcag ccatgtgagc
     3421 ggcgggcggc tgtggagcgt caaccggctc gccaacgagc tggggctgcc cggcatcgcg
     3481 gtcgcgcaga tggcgaccgc gctcgagcgc gctggactcg tgatcgttac cgaagacgat
     3541 gagctggtgc cggcgcgcga tatcggcggc atcggcgtga tcgagatcct cgacatcgcg
     3601 cgcaatcagc gcagcggcca tctcgtgccg cgcaacctgc ccgtgccacc ggtcgaccgc
     3661 gtgctggcga gcgtcgag
//
LOCUS       DATDMP010000227         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1701564, whole genome shotgun sequence.
ACCESSION   DATDMP010000227 DATDMP010000000
VERSION     DATDMP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701564"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VI195_03855"
     CDS             <1..247
                     /locus_tag="VI195_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823546.1"
                     /translation="SKEQLITELLGAALKELAAAMPYVAGGRVISTDEHGDPVYEDVG
                     PTPRFMELTRLHRRTLEARRQRAVRPKKKRRPRRASR"
     gene            252..734
                     /locus_tag="VI195_03860"
     CDS             252..734
                     /locus_tag="VI195_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY6823547.1"
                     /translation="MPSFDAVSELNSHEVANAVDQANRELAQRFDFKDTGARFELRDF
                     SVTLHAQVDFQLKQMLEILKLRLSKRGIDLGCLELKEPHTTLSSAQQEVVLRHGIDAD
                     TGRKITRLVKDSKLKVQASIQGDKVRITGKQRDDLQAAIALLRTAKLDVPLQFTNFRD
                     "
     gene            complement(715..1647)
                     /locus_tag="VI195_03865"
     CDS             complement(715..1647)
                     /locus_tag="VI195_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEY6823548.1"
                     /translation="MSRPALLAPVRVLMLGALCVGQAFSTEPGARKPLRTVPDDLGRA
                     VAVAHPALRIVSLTPGATEMLFAAGAGGQLIATVQYSSEPAAARTVPRIGDVAAIDME
                     RLVALQPDVVIAWPAGGNPAQRAKIAALGIPLYQQQVARLADLPGSLRRLGVLAGTEA
                     VAEPAAAALEARLAALERTYSGPSAQGHRPTVLLQVWNRPIYTIGGRHLMSDALELCG
                     ARNVFADLPEPGPLVATEAVIGRNPDIILAAAPPGEGAAWVADWQRLPGLAAVRNHRV
                     VAFENQALSRLGPSVLDATEDLCRTLARVSRGSW"
     gene            complement(1673..>2039)
                     /locus_tag="VI195_03870"
     CDS             complement(1673..>2039)
                     /locus_tag="VI195_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823549.1"
                     /translation="TLWVVLISVLDRALRLLLAGYAAAEPTMSFTLGMLLSRLAIGAL
                     TSLAAGAIAAWIAPASTRLPVLLGITLLVAFIPVHARLWSLFPFWYHLVFLGTLLPLV
                     LLGSRLPRLRIMAEARARS"
BASE COUNT          381 a          699 c          690 g          269 t
ORIGIN      
        1 ctcgaaggag caactgatca ccgagcttct cggcgcagcg ctcaaggaac tcgccgcggc
       61 catgccctat gtcgccggcg gccgcgtgat ctccaccgac gagcacggcg atccggtgta
      121 cgaggacgtg ggcccgacgc cgcgcttcat ggagctcacc cgtctccacc gccgcactct
      181 cgaggctcgc cggcagcggg cggtgcggcc gaagaagaag cgccgcccgc ggcgcgcctc
      241 gaggtaactg catgccctcc ttcgatgcgg tctccgagct caactcccac gaggtagcca
      301 acgccgtcga ccaggcgaat cgcgagctgg cgcagcgctt cgatttcaag gataccggtg
      361 cgcgtttcga gctcagggac ttcagcgtca cgctgcacgc gcaggtggac ttccaactca
      421 agcagatgct cgagatcctc aagctgcgcc tctccaaacg cggcatcgat ctcggctgtc
      481 tcgagctgaa agagccgcac actacgctct cgagcgccca gcaggaggtg gtgctgcgcc
      541 acggtatcga tgccgacacc ggccgcaaga tcacgcggct ggtgaaggac tccaagctca
      601 aggtgcaggc gagcatccag ggcgataagg tgcgcatcac cggcaagcag cgcgatgacc
      661 tgcaggcagc gattgcgctg ctgcgcaccg ccaagctgga tgtgccgctg cagttcacca
      721 acttccgcga ctgacacggg caagcgtgcg gcacagatcc tcggtggcgt cgaggacgct
      781 gggaccgaga cggctgaggg cctgattctc gaacgcgacc acgcggtgat tacgcaccgc
      841 ggcgaggcca ggcaggcgct gccagtcggc gacccacgcc gcgccctccc cgggcggagc
      901 ggcagccaga atgatgtccg gattgcggcc gatgaccgcc tcggtagcca cgagcgggcc
      961 tggctcggga agatctgcaa acacattacg cgccccgcag agttcgagcg catcactcat
     1021 caggtgccgg ccaccgatcg tgtagattgg tcggttccac acctgcagca gcaccgtcgg
     1081 ccggtgtccc tgcgcgctcg ggccggagta ggtgcgctcg agggcggcga ggcgtgcttc
     1141 gagcgccgcg gcagctggct cggccaccgc ctcggtgcca gcgagcacac ccagccgtcg
     1201 aagcgagcct ggaaggtcgg cgaggcgcgc cacctgctgc tgatagaggg gaatgccgag
     1261 tgcggcgatc ttcgcgcgct gggcgggatt gccgcccgcc ggccacgcga tcaccacatc
     1321 cggctgcagc gccacgagtc gctccatgtc gatcgccgcc acatcgccga tgcgcggcac
     1381 ggtgcgcgcc gcagcgggct cgctcgagta ctgcacggta gcgatgagtt gcccgcccgc
     1441 accggccgca aacagcatct ccgtggcacc cggtgtgagg ctgacgatgc gtagcgccgg
     1501 atgcgccacc gcgaccgcgc ggccgagatc gtcgggcacg gtacgcaggg gtttgcgcgc
     1561 accgggctcc gtggaaaacg cctgccctac gcacaaggcg ccgagcatca gcactcggac
     1621 gggcgcgagc aatgcaggac gactcatcgt cggtgcggat accggcgcag agtcaggaac
     1681 gagcgcgcgc ctcggccatg atgcgcagac gcggcaggcg cgatcccagc agtaccagcg
     1741 gcaacagcgt gcccaggaag accaggtggt accagaaagg gaacaggctc cagagtcgcg
     1801 catggaccgg aatgaaggcg accagcagcg ttatgccgag cagcaccggc aggcgcgtgc
     1861 tggcgggcgc aatccacgcc gcgatcgccc cggcggcgag cgaggtgagc gccccgatcg
     1921 cgagtctcga caacaacatg ccgagagtga agctcatcgt gggctcggct gccgcataac
     1981 ccgccagcag cagccgcaga gcacggtcca acaccgagat gagcacgacc cagagcgta
//
LOCUS       DATDMP010000228         4520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1812388, whole genome shotgun sequence.
ACCESSION   DATDMP010000228 DATDMP010000000
VERSION     DATDMP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812388"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VI195_03875"
     CDS             <1..933
                     /locus_tag="VI195_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6823550.1"
                     /translation="SYHGLFDAAMWYTPMEKWSQVGDTVVYPYSEGVPLATRNFAHFV
                     QANDANQLEDVLRRHGSKIACLLVEPIMGNCLGIAAEPEYVRAARALCDRYGVVMIID
                     EVKTGFRVARGGVQELYGVRADLCTFAKAIANGYPISVLAGREDIMRKLGKGVAHGGT
                     YTAHPVSLAAAEKTLEILAETDALERIAAYGTRLRQGKSAILKARGIAHSFVGHPSMS
                     GLYFAHDPPRTYRDWKRSDYTFYDAVARVLHDECILCEPDSREPWFMSAAHDDSCLED
                     TLRAFEVAVDRTLDTLPGRGRGREWGLMRGEPAA"
     gene            930..2552
                     /locus_tag="VI195_03880"
     CDS             930..2552
                     /locus_tag="VI195_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6823551.1"
                     /translation="MNASAANRYDAIVVGAGHNGLVNACYLARAGLKVLVLERNPYVG
                     GAAVSRKLYDGFTYSNCSYVCSLLRTEIMRALELPQYGLQIIPYEGGCTFMSNGEHLA
                     IYDNHDALRREIARHSRHDAEAYERYVRDMLRHCRFIKPLLLRTPPDPTSLRVRDLQE
                     LLFLGRHFYKLGEARMYDTLRFFTMSCADMLEEYFESEIVKAHLAGSSIIGTALGPRS
                     PGTAYVLLHHYMGSIDGAVGAWGFARGGMGAITQALTASLTASGGTVRTAAPVEQILV
                     RNGRTLGVVLEDGEQFSAPIVVSNLDVKRTFLNTVEAHELPPEFLAQVRRFKIRGSSG
                     KLNIALDGLPHFPAIPQGSPCIRGDMHVTDTLEMVERAYDDWKEGRWSRAPYIDMLIP
                     TQIDPSMTPDGKHYMSVFVQYCPYRLAEGEWTAQKRQAFGDTVIDTIARHSPDFRKLI
                     LHAEIRTPQEIENEVGLTEGNIFQGELTFDQLFFNRPLPGYAQYRSPLRGLYMCGSST
                     HPGGGVMGAPGANCAHAILQDMGRLPAQGRPS"
     gene            2549..4123
                     /locus_tag="VI195_03885"
     CDS             2549..4123
                     /locus_tag="VI195_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6823552.1"
                     /translation="MSATAGARYDSIVIGGGHNGLVCAAYLARSGRRVLVLEAASQVG
                     GAAVTREFAPGFKVSACAHLLHLMPPGLARELALEAHGLDLAASDLPSTALAADGAHL
                     SLGTEAVRALSAHSAADSAAYADWQALLARLAAALHPVLDGAPPRLGTDGWRERAALL
                     GLGWRLRRLGRRDLRELLRVGGMCVHDLLEERFQLPLLKGALALDAVLGTNYGPRSPG
                     TVFSLLYRAAAGQGYGRFVQPRGGLGALSQSLARAATAAGAEIRTASAVERIIVREDR
                     AAGVQLVSGEAISAGTVVSSADPKTTFLRLLGCEHLDAGFVRRVVQLRSRGLTAKLHL
                     ALSRLPEFPELPASALRGRLLIAPSSDYIERAYNHAKYNEFSTAPVLELTLPTLADPA
                     LAPQGRHVLSAIVQYAPYTLEGSWMGQRRRFTDIIVETLARYAPELPDCIVASELLAP
                     PDIEREFRISGGHWHHAELALDQFFMVRPVPGATQYRAPLAGLYLCGAGSHPGGGVMG
                     VAGRNAARALIRAKAA"
     gene            4125..>4520
                     /locus_tag="VI195_03890"
     CDS             4125..>4520
                     /locus_tag="VI195_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823553.1"
                     /translation="MLTTSEPHYRQPLRKTPFHERARALSQLDSFIPWSGYTTVDVFT
                     SVEQEYFAVRNASSLYDLTPMVKYRIVGADALRFLNRLTTRDLSKLAPGRVAYTVWCN
                     DAGHLIDDGTVFCMGPDDYRLCTAEWQLDW"
BASE COUNT          700 a         1579 c         1504 g          737 t
ORIGIN      
        1 agctatcacg ggctgttcga cgccgccatg tggtacacac caatggagaa gtggtcgcag
       61 gtgggcgata cggtcgtcta tccgtacagc gaaggcgtgc cgctcgccac gcgcaacttc
      121 gcgcacttcg tgcaggcgaa cgacgccaac cagctcgagg acgtactgcg ccggcatggg
      181 tcgaagatcg cctgcctgct ggtcgagccc atcatgggca actgccttgg catcgccgcc
      241 gagccggagt acgtgcgcgc ggcgcgcgcg ctgtgcgacc gctacggcgt cgtcatgatc
      301 atcgatgagg tgaagaccgg cttccgcgtc gcgcgcggcg gcgtgcagga gctctacggg
      361 gtgcgggccg atctgtgtac cttcgccaag gccatcgcca acggctaccc gatctccgtg
      421 ctcgccggac gcgaggacat catgcgcaag ctcggcaagg gggtcgcgca tggcggcacc
      481 tacaccgccc atccggtctc gctggcggca gcagagaaga ctctcgagat tctcgccgag
      541 accgatgcgc tcgagcgcat cgccgcctac ggcacgcggc tgcgccaggg caagagcgcg
      601 attctcaagg cgcgcggcat cgcccacagc ttcgtcgggc atccttccat gagcgggctc
      661 tacttcgctc acgatccgcc gcgcacctac cgcgactgga agcgcagcga ctacaccttc
      721 tatgacgccg tggcgcgggt gctgcacgat gagtgcatcc tgtgcgagcc ggactcgcgc
      781 gagccgtggt tcatgtccgc ggcgcacgat gactcgtgcc tcgaggacac gctgcgggcc
      841 ttcgaggttg cggtcgaccg tacgctcgat actctgcccg gcaggggccg cggtagggag
      901 tgggggctga tgcgcggcga gccggcggcg tgaatgccag cgctgccaac cgctacgacg
      961 cgatcgtcgt cggggcgggg cacaacgggc tggtgaatgc ctgctacctg gcgcgcgccg
     1021 ggctcaaggt gctcgtgctc gagcgcaacc cctacgtcgg cggggccgcg gtgagccgca
     1081 aactctacga cggcttcacc tactcgaact gctcctacgt ctgcagcctg ctgcgcacgg
     1141 agatcatgcg tgcgctcgag ctgccgcaat acggcttgca gatcatcccc tacgagggcg
     1201 gctgcacctt catgagcaac ggcgagcatc tggcgatcta cgacaaccac gacgccctgc
     1261 gccgtgagat cgcccgccac tcgcgccacg atgcggaagc ctacgagcgc tacgtgcgcg
     1321 acatgctgcg ccattgccgt ttcatcaagc cgctgctgct gcgcaccccg cccgatccaa
     1381 cctccttgcg ggtgcgcgac ctgcaggagc tactgttcct cggccgtcac ttctacaagc
     1441 tcggcgaggc gcgcatgtac gacacgctgc gcttcttcac catgagctgc gccgacatgc
     1501 tcgaggagta tttcgagagc gagatcgtca aagcgcacct cgccggcagc tcgatcatcg
     1561 gcacggcact cggtccgcgc tcgcccggaa cggcttacgt gctgctgcac cactacatgg
     1621 gcagcatcga tggagcggtc ggcgcctggg gcttcgcacg cggcggcatg ggcgccatta
     1681 cccaggcgct cacggcctca ttgaccgcca gcggcggtac ggtgcgcacc gcggcgccgg
     1741 tcgagcagat cctggtgcgc aacggccgca ctctcggtgt ggtgctcgag gatggcgagc
     1801 agttcagcgc gccgatcgtg gtctccaatc tcgatgtcaa gcgcaccttt ctcaacaccg
     1861 tggaggcgca cgagctgccg ccggaattcc tcgcgcaggt acggcgcttc aagattcgtg
     1921 gctcctcggg caagctcaac atcgcgctcg atggtctgcc gcacttcccc gcgattccgc
     1981 agggctcccc gtgcatacgc ggggacatgc acgtgaccga cacgctggag atggtcgagc
     2041 gcgcctacga cgactggaag gagggacgct ggtcgcgtgc gccttacatc gacatgctca
     2101 ttccgaccca gatcgatccg agcatgacac ccgatggcaa acactacatg tcggtgttcg
     2161 tgcaatactg tccttatcgg ctcgccgagg gggaatggac cgcgcagaaa cgccaggcgt
     2221 tcggcgacac cgtgatcgac accatcgcac ggcacagccc ggatttccgc aagctgatct
     2281 tgcacgcgga gatccgcacc ccgcaggaga tcgagaacga agtgggcctc accgaaggca
     2341 acatctttca gggtgagctg accttcgatc agctgttctt caaccgcccg ctgcccggat
     2401 acgcccagta ccgctcgccg ctgcggggat tgtacatgtg cggttcgagc acccatccgg
     2461 gcggcggggt catgggcgca ccgggtgcca actgtgcaca cgccatcctg caggacatgg
     2521 ggcggctgcc ggcgcagggc aggccctcgt gagcgcaacc gccggcgcac gttacgacag
     2581 tatcgtcatc ggcggcggtc acaacgggct cgtgtgcgcc gcgtacctcg cccgcagcgg
     2641 ccgcagggtg ctcgtgctcg aggccgcttc ccaggtgggc ggggctgccg tcacccgcga
     2701 attcgcgccg ggattcaagg tctcggcctg tgcgcacctg ttgcacctga tgcccccagg
     2761 gctcgcgcgt gagctcgcgc tcgaggcaca cgggctggat ctggcggcga gtgacctgcc
     2821 gagcaccgcg ctcgcggccg acggtgcgca cctctcgctc ggcaccgagg cagtgcgcgc
     2881 actgagcgca cactcggccg ccgactccgc cgcctacgcg gactggcagg cgctgctcgc
     2941 gcggctggcg gcggcgctgc atccggtgct cgatggcgct ccgccgcgtc tcggcaccga
     3001 tggctggcgc gagcgcgcgg cgctgctggg cctcggttgg cgtctgcggc gcctcggacg
     3061 gcgcgacctg cgggaactgc tgcgtgtcgg cggcatgtgc gtgcacgacc tgctcgagga
     3121 gcgcttccag ctgccgttgc tcaagggcgc gctggcgctc gatgcggtgc tcggcacgaa
     3181 ttacggcccg cgctcccccg gcaccgtgtt ctctctgctg taccgcgccg cggcgggaca
     3241 gggttacggg agattcgtgc agccgcgcgg cggtcttggg gcgctgtcgc agtcgctcgc
     3301 gcgcgcggcg accgccgcgg gcgccgagat ccgcaccgcg agcgccgtcg agcgcatcat
     3361 cgtgcgtgag gaccgcgccg ccggtgtgca gctggtctcg ggcgaggcga tcagtgccgg
     3421 cacggtcgtc tcgagcgccg atcccaagac gactttcctg cgactgctcg gctgcgagca
     3481 cctcgatgcg ggcttcgtgc gccgcgtcgt gcagctgcgc tcgcgcggac tcaccgcgaa
     3541 gctgcacctg gcgctgtcgc gacttcccga gttcccggag ctcccggcga gcgcgctgcg
     3601 cggacggctg ctgatcgcgc cttcatccga ctacatcgag cgggcctaca accacgccaa
     3661 gtacaacgag ttctcgacgg cgccggtgct cgagctgaca ctgccgacgc ttgccgatcc
     3721 agcgctcgcg ccgcaggggc ggcatgtgct ctccgcgatc gtgcagtatg cgccctacac
     3781 gctcgagggg agctggatgg ggcagcgacg gcgcttcacc gacatcatcg tcgagacact
     3841 cgcgcgctac gctccggagc tgcccgactg catcgtcgcg agcgagctgt tggcaccgcc
     3901 tgacatcgag cgcgagttcc gcatcagcgg cggtcactgg catcatgccg agctcgcact
     3961 cgatcagttc ttcatggtgc gcccggtgcc gggtgccacg cagtaccgcg cgccgttagc
     4021 gggcctctac ctgtgcggcg ccggcagcca tccgggcggc ggcgtcatgg gggtcgccgg
     4081 tcgcaacgcc gctcgggctc tcatccgcgc gaaggccgcc tgacatgctc accacgagcg
     4141 aaccgcacta ccggcagccg ctgcgcaaga cgccgtttca cgagcgcgcg cgtgcgctca
     4201 gccagctcga cagcttcatt ccctggagcg gctacacgac cgtcgatgtg ttcacgagcg
     4261 tagagcagga gtacttcgcg gtccgcaacg cgagctcgct ctacgacctg acacccatgg
     4321 tgaagtaccg catcgtcggc gccgatgcac tgcgcttcct caaccgcctg accacccgcg
     4381 atctgagcaa gctggcgccc ggccgggtgg cctacaccgt ctggtgcaac gacgccggcc
     4441 acctcatcga tgatggcacg gtgttctgca tgggccccga cgattaccgc ctgtgcaccg
     4501 ccgagtggca gctcgactgg
//
LOCUS       DATDMP010000229         2503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1368533, whole genome shotgun sequence.
ACCESSION   DATDMP010000229 DATDMP010000000
VERSION     DATDMP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368533"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VI195_03895"
     CDS             complement(<1..249)
                     /locus_tag="VI195_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6823554.1"
                     /translation="MPSIRASVDGSSGELAPVLTLRGLSVTFDTAQGPLRAVQEVTLA
                     VASGECLGIVGESGAGKSQLFLAVMGLLAANGRTSGSAR"
     gene            complement(224..1093)
                     /locus_tag="VI195_03900"
     CDS             complement(224..1093)
                     /locus_tag="VI195_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6823555.1"
                     /translation="MNEAAAASLGLWSAARRRLRSQLAVNVALAVLAVLALAALLAPA
                     LSPWHYDSLDWQHLAAPPGAAAAHWLGTDRLGRDLLIRTLYGLRLSLLLALIASTVSL
                     LIGVLWGAVAGLAGGRIDGAMMRCVDVLYSLPYLFVVIILTTLFPRGSLAVLALALAA
                     VGWLTTARIVRAQTLALRRREFIEAARATGVPPLGILWRHIVPNVLGPVAVYATLIVP
                     QMILFESFLSFLGLGVQEPQASLGNLIFAGAQEMESAPWMLLVPATALVLLLLCLNLL
                     GDGLRDAFDPRER"
     gene            complement(1086..2009)
                     /locus_tag="VI195_03905"
     CDS             complement(1086..2009)
                     /locus_tag="VI195_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6823556.1"
                     /translation="MLKLALRRIAALIPTLLLIVTVSFAIIRLAPGGPFDAERGVSPA
                     VRANLERLYGLDAPLALQYLRYVRALAHGDFGPSLRQRDFTVSELIRQGLPLSAALGL
                     CAIALALITGIPAGIAAALWRGAGADLAIAATGALAVALPSFVIGPVLALIFGIYLRW
                     LPVAGWQPGEPRYLLLPVVTLALPLALALARLTRASLLEVLRAPFVRSARARGLGGRR
                     VLWRHALRPALLPVASYLGPATAFVITGSLVVEAVFGLPGSGRYLVQGAIDRDYPLVM
                     GMVIVYGVLTLLLNLLADLACGWLDAGTPHE"
     gene            2125..>2503
                     /locus_tag="VI195_03910"
     CDS             2125..>2503
                     /locus_tag="VI195_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY6823557.1"
                     /translation="MKRILVGIKRVVDANVRIRVKPDGSGVVTDGVKMAINPFDEIAL
                     EEALRIKERGAAEEIIVATVGPADCQQQLRTALAMGADRALHVQADGAIEPLSVARAL
                     LKLIEREQPLLVILGKQAIDDDNG"
BASE COUNT          488 a          848 c          878 g          289 t
ORIGIN      
        1 ccgcgcgctg ccgctcgtgc ggccattggc ggcgagcagt cccattaccg cgagaaacag
       61 ctgactcttg ccggcgcccg actcgccgac gatgccgagg cactctccgc tcgcgaccgc
      121 gagcgtgacc tcctgcaccg cccgcagcgg cccctgtgcg gtatcgaagg taacgctgag
      181 gccgcgcagc gtcagcaccg gcgcgagctc acccgaggag ccgtcaacgc tcgcgcggat
      241 cgaaggcatc tcgcaggcca tcgccgagca gattcaggca cagcagcagc agcaccagcg
      301 cggtcgccgg cacgagcagc atccagggcg cggactccat ctcctgtgcc ccggcgaaga
      361 tcagattgcc gaggcttgcc tgcggttcct gcaccccgag cccgaggaag gagaggaagc
      421 tctcgaagag gatcatctgc ggcacgatca aggtcgcgta aaccgccacc gggcccagca
      481 cgttcggcac gatgtgccgc cacagaatgc caagcggtgg cacgccggtg gcgcgtgccg
      541 cctcgatgaa ctcgcgccgc cgcagtgcga gcgtctgcgc gcgcacgatg cgcgcggtcg
      601 tgagccagcc gaccgcagcg agcgcgagcg cgagcaccgc gaggctgccg cgcgggaaca
      661 gcgtggtgag aatgatcact acgaaaagat acggcagcga atacaacaca tcgacgcagc
      721 gcatcatcgc gccatcgatg cgtccaccgg caaggccggc taccgctccc cagagcacgc
      781 cgatcagcag gctgacggtg ctcgcgatca gggcgagcag cagcgacagc cgcaggccgt
      841 aaagcgttct gatcaagagg tcgcgtccga gccggtcggt accgagccaa tgcgcggcag
      901 ccgcccccgg tggcgcggcg aggtgctgcc agtcgaggct gtcgtagtgc caggggctca
      961 gcgcgggagc gagcagtgcc gcgagcgcga ggacggcgag cacggcgagc gcgacattga
     1021 cggcgagctg gctacgcaga cgccggcgcg ccgcgctcca gagcccgaga ctcgcagccg
     1081 cggcttcatt catgcggggt gcccgcatcg agccagccgc aggcgagatc ggcaagaaga
     1141 ttgaggagca gcgtcagcac cccgtacacg atcaccattc ccatgaccag cgggtagtca
     1201 cggtcgatgg cgccctgcac caggtagcgt ccgctaccgg gcagaccaaa caccgcctcg
     1261 acgacgagcg agccggtgat gacgaacgcg gtggccggcc ccaggtagct cgcgaccggc
     1321 agcagcgcgg gacgcaacgc gtggcgccac agcacccgcc ggccaccgag gccgcgcgca
     1381 cgggcactgc gcacgaaggg cgcgcgcagc acctcgagga gactcgcacg cgtcaagcgg
     1441 gcaagcgcca gggcaagcgg cagtgccagc gtcaccaccg gcaagagcag gtaacgcggc
     1501 tcgcccggct gccagcccgc caccggcaac caacgcaggt agataccgaa gatgagtgcc
     1561 agcaccggcc cgatgacgaa gctcggcagg gccaccgcga gcgcgccggt tgcggcgatg
     1621 gcgaggtcgg cgcctgctcc gcgccacagc gccgcggcaa tgccggcggg aatgccggtg
     1681 atgagcgcca atgcaatggc acacaacccg agcgcggcac tcaacggcaa tccctggcga
     1741 atgagctcgc tcaccgtgaa gtcgcgctgg cgcagcgaag gcccgaagtc accgtgcgcc
     1801 aacgcacgca cgtagcgcag gtactgcagc gccagtggcg catcgaggcc gtagaggcgc
     1861 tcgagattgg cccgcacggc ggggctcacg ccccgctccg catcgaaggg gccgcccggg
     1921 gcgagacgga tgatggcgaa ggagacggtg acgatgagga gcagtgtggg aatgagcgca
     1981 gcgatgcgac gcaacgcgag cttcagcatg cagggactct agcacgtcac ggcgggcacg
     2041 attcgtgagg gtcaacatcg agcgttgcct gccgctatac tgccctttcc gcccgtgtgc
     2101 cgggtgcatg aagagcgaag aggaatgaag cgcatcctgg tcggcatcaa gcgcgtcgtg
     2161 gacgccaacg tgcgcatccg cgtgaagccc gatgggagcg gcgtcgtcac cgatggggtg
     2221 aagatggcca tcaacccctt cgacgagatc gcgctggagg aggcattgcg catcaaggag
     2281 cgcggcgccg ccgaggaaat cattgtcgcc acggtcggtc cggcagactg ccagcagcag
     2341 ctgcgtaccg cgctcgccat gggcgccgac cgcgcgctac acgtgcaggc ggatggagcg
     2401 atcgagccac tgagcgtggc gcgcgcgctg ctgaagctca tcgagcgcga gcagccgctg
     2461 ctggtgatcc tgggcaagca ggcgatcgat gacgacaacg gcc
//
LOCUS       DATDMP010000230         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_35413, whole genome shotgun sequence.
ACCESSION   DATDMP010000230 DATDMP010000000
VERSION     DATDMP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35413"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VI195_03915"
     CDS             <1..604
                     /locus_tag="VI195_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEY6823558.1"
                     /translation="QYYGWAWLNRAQLLPSRAQLERAEAVTRAFRERVGTRMQIYFVV
                     PDYFETRPKACMNGLGSVFLAISPDGTALPCHAARMLPGLTFPNVRELPLRSIWYESE
                     GFNHFRGDAWMKEPCRSCPEKHRDFGGCRCQAYLLTGDAANADPVCELSPHHALVTEA
                     VARAERAAAGGTPQPGAALVFRDHRISIAVVPPARNGVAS"
     gene            601..1386
                     /locus_tag="VI195_03920"
     CDS             601..1386
                     /locus_tag="VI195_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY6823559.1"
                     /translation="MTLETLSEHRCFGGVQGFYRQPSAVTGTPMRFAVYQPPQARERR
                     VPVLFYLAGLTCTEETFTIKAGAQRLAAQHGLMLVTCDTSPRNTGIPGEADDWEFGSG
                     AGFYLNATQPPWSKYFRMYDFVLEELRAMVLALRNPTVYRSVSAFAPICSPSRCPWGV
                     KALGRYLGEDRSGWANYDAVELLKARGARTFPEILVDQGQADKFLAQGQLLPELLESA
                     CAAAGQPLRLRRHEGYGHDYYFIATFMEDHVRFHAEALSRVAA"
     gene            1518..>2231
                     /locus_tag="VI195_03925"
     CDS             1518..>2231
                     /locus_tag="VI195_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6823560.1"
                     /translation="MLLAALLAACAAAPPHAAPPDPAQTLAGFDARRLDALPGLPPAA
                     QGWDRAQWLDAALQLNPALAAERSRLSAVAAAERTAAEHPNPTLDLFAEYLTTAAHSS
                     AWLYGLSLDFLLRQPGERARIRRRAALETELAQSELAESIWQVRSQLRRALLDAVAAQ
                     DESALLESLVAGRQGLLDIDRDRVQLGDIPRTQLLATEAELSHAEARRAQSQARRADA
                     LARLAAAVGVPAAALAAVPV"
BASE COUNT          297 a          824 c          783 g          327 t
ORIGIN      
        1 ccagtactac ggctgggcat ggctcaaccg ggcgcagctg ctgccgagcc gcgcgcagct
       61 cgagcgcgcc gaggccgtga cccgcgcgtt ccgcgagcgc gtgggcaccc ggatgcagat
      121 ctacttcgtg gtgccggact atttcgagac gcgtcccaag gcctgcatga acggcctggg
      181 gagcgtgttt ctcgcgatca gccccgacgg caccgcgttg ccgtgccacg cggcgcgcat
      241 gctgcccggc ctcaccttcc ccaacgtccg cgagctcccc ctgcgcagca tctggtacga
      301 atcggaaggc ttcaatcact tccgcggcga tgcctggatg aaggagccgt gccgcagctg
      361 tccggagaag caccgcgatt tcggcggctg ccgctgtcag gcgtacctgc tgaccggcga
      421 tgcggccaac gccgacccgg tatgcgagct gtcgccgcat cacgccctgg tcaccgaggc
      481 ggtagcgcgc gccgagcgcg ccgccgcggg cggcacgccg cagcccgggg cggcgctggt
      541 gttccgcgat caccgcatct cgatcgccgt ggttcccccg gcgcgcaacg gagtggcctc
      601 atgacgctcg aaacgctcag cgagcaccgc tgcttcggcg gcgtgcaggg tttctatcgg
      661 cagccgtcgg ccgtgacggg tacgccgatg cgcttcgccg tgtatcagcc gccgcaggcg
      721 cgcgagcgcc gcgtcccggt gctgttctac ctcgcggggc tcacctgcac cgaggagacc
      781 ttcaccatca aggcgggcgc gcagcgcctc gccgcgcagc acggactgat gctggtgacc
      841 tgcgacacca gcccgcgcaa caccggcatc ccgggcgagg ccgacgactg ggaattcggc
      901 agcggcgcgg gcttctacct caacgcgacg caaccgccct ggtcgaagta cttccgcatg
      961 tacgacttcg tgctcgagga gctgcgcgcc atggtgctgg cgctcaggaa ccccaccgtg
     1021 taccgctcgg tgtcggcctt cgcgccgatc tgctcgccgt cacgctgccc gtggggagtg
     1081 aaggccctgg ggcgctatct cggcgaagac cgctcgggct gggcgaatta cgatgccgtc
     1141 gagctcctca aggcgcgcgg cgcgcgcacc ttccccgaga tcctggtcga tcagggtcag
     1201 gccgacaagt tcctcgccca gggacagctg ctgccggagc tgctcgagtc ggcctgcgcc
     1261 gcggccggtc agccgctgcg tctgcgccgt cacgagggct acggccacga ttactacttc
     1321 atcgccacct tcatggagga tcacgtgcgc tttcatgcgg aggcgctcag ccgcgtcgcg
     1381 gcctgaaggg actgcgccgt tgcagcacgg ccgccctata ctgcgccgcg ggagagcggt
     1441 cggtgcagcg atcaggacag gcgttgtgtc aggtgccggc ggacacgtgt cgcgcagcgt
     1501 gcgcggcgct gccggcgatg ctgctcgcag ccttgctggc ggcctgcgcc gctgcgccgc
     1561 cacacgcagc gccgcccgac cccgcgcaga cgctcgccgg cttcgatgca cggcgcctcg
     1621 atgcgctgcc gggcctgccg ccggccgcgc agggctggga ccgggcgcag tggctcgatg
     1681 cggccctgca actcaatccg gccctcgccg ccgagcgctc gcgcctcagc gcggtcgccg
     1741 ccgccgagcg caccgccgcc gagcatccca atcccacgct cgacctgttc gccgagtacc
     1801 tgacgacggc ggctcacagc agcgcgtggc tctacggact gtcgctcgat ttcctgctgc
     1861 gccagccggg cgagcgcgcg cgcatccgcc gccgggcggc gctcgagact gagctggcgc
     1921 aatcggagct cgccgagtcg atctggcagg tgcgctcgca actgcgccgg gcgctgctcg
     1981 atgcggtagc ggcgcaggac gagtcggcgc tgctcgagtc cctcgtcgcc gggcggcagg
     2041 ggctgctcga catcgatcgg gaccgcgtgc agctcggtga catcccgcga acgcagctgc
     2101 tcgcgaccga agcggagctc tcgcacgccg aggcgcgccg cgcacagtcg caggccaggc
     2161 gtgccgacgc gctggcgcgg ctcgccgccg cggtcggagt gcccgcggcg gcgctcgcgg
     2221 cggtgccggt g
//
LOCUS       DATDMP010000231         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2146334, whole genome shotgun sequence.
ACCESSION   DATDMP010000231 DATDMP010000000
VERSION     DATDMP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146334"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /gene="lptD"
                     /locus_tag="VI195_03930"
     CDS             <1..1891
                     /gene="lptD"
                     /locus_tag="VI195_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY6823561.1"
                     /translation="MSLTPQGTLRLKGVSFTTCPANDRSWRLRADSITLDTRSKLGTG
                     RDARVDFFGVPLMYLPWVSFPLSSERKSGFLFPSIGNTSTSGLQLAVPYYWNIAPNAD
                     FTFQPVEYSKRGADLGGDLRFLTASQRGELEWNYLPNDSVFHGSRSRLALSDVAELPA
                     DFRLTLAAQNVSDDLYFQDFSQSPEGTSTAFLERRATLSYRGEHWSFDGEAQQYQTID
                     TTLLVDERPYTRAPRLALKSGYTLGNVLHYGFDSEIVDFQHAASVPGEPLLPTGWRAD
                     VTPGLALDLAGPGYFLRPAFAWRGTEYQLDNTAPGAPRSPSRTVPIASLDTGLAFERA
                     SGSRGERTLTLEPRLLYLDVPYRNQDALPVFDSALPDLNPVELFRTNRYVGADRVSDA
                     DQLSFGVTSRLLDSLDGRQFLAATFGQTYYFRTPRVVLPGETPLTGRHSDMVATVALT
                     AFQHWGADLGVQWDPQNERSERTEVNLQYKPAGDAVVNLSYRYERFEQLPDIIQGVTL
                     LCSPLLSPAGCNQQGYDQVDFSSAWPIRRNWAVFARDVYDLRHGEQLEEFAGFEYRAC
                     CWRLRLGARRYVSSFTGARDTGVWLQLELAGLAGVGSASDTSLSEEIRGYTPPEANTR
                     RIRGP"
     gene            1897..>2568
                     /locus_tag="VI195_03935"
     CDS             1897..>2568
                     /locus_tag="VI195_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY6823562.1"
                     /translation="MVDSRTHPMRRILTPASLALVLGAVALAAYTPSRAQTRELETRG
                     VLLDRIAAIVNDGVVLTSELDQQVAAVSERLRTQKLELPPQNVLRQQVLDRLVLQEIQ
                     MQRANHDGIKVSDETLNAALQEVAKRNNLTLSQLPDALAAQGIDYAAYRDDLRREITL
                     GMLRQRDVLDHISVTPREIDQYLEKQARMPAQNSEYNVSHILIAVPQEATPAQIDQAA
                     RRAAEV"
BASE COUNT          411 a          955 c          835 g          367 t
ORIGIN      
        1 catgagcctc accccgcagg gaacattgcg cctgaagggc gtgagcttca cgacctgccc
       61 cgccaacgac agatcgtggc ggctccgggc cgacagcatc accctcgaca cgcgcagcaa
      121 gctcggcacg gggcgcgatg cgcgcgtcga cttcttcggc gtgccgctca tgtatctgcc
      181 gtgggtgtcc ttcccgctga gcagcgagcg caagagcggt ttcctcttcc ccagcatcgg
      241 caatacctcg accagcggcc tgcagctcgc ggtgccgtac tactggaaca tcgcgcccaa
      301 cgccgatttc acctttcagc cggtcgagta cagcaagcgc ggcgccgatc tcggcggcga
      361 cctgcgcttc ctcacggcga gtcagcgcgg ggagctcgag tggaactatc tgcccaacga
      421 cagcgtgttc cacggcagcc gcagccggct ggcgctgagc gacgtggcgg agctgccggc
      481 cgacttccgc ctgacgctcg cggcgcagaa cgtcagcgac gacctctact tccaggactt
      541 ctcgcagagc ccggaaggca ccagcaccgc atttctcgag cgccgcgcga cgctctcgta
      601 ccgcggcgag cactggagct tcgacggcga ggcccagcag taccagacga tcgacacgac
      661 gctgctggtc gacgagcgcc cgtacacgcg cgcaccgcgg ctcgcgctca aaagcggcta
      721 caccctcggc aacgtgctgc actacggctt cgattccgag atcgtcgact tccagcacgc
      781 cgcgtcggtg ccgggcgagc cgctgctgcc gaccggctgg cgcgccgacg tcacgcccgg
      841 cctggcgctc gatctcgccg ggccgggcta tttcctgcgc cccgcgttcg cctggcgcgg
      901 caccgagtac cagctcgaca acaccgcgcc gggcgcgccg cgttcgccct cgcgcaccgt
      961 gcccatcgcc agcctcgaca ccgggctggc attcgagcgc gccagcggct cgcgcgggga
     1021 gcgcacgctc actctcgagc cgcgcctgct gtacctcgac gtgccctacc ggaaccagga
     1081 cgcgctgccg gtcttcgata gcgcgctccc cgatctcaat ccggtcgagc tgttccgcac
     1141 caaccgctac gtgggcgccg accgcgtgag cgacgccgac cagctgagct tcggggtgac
     1201 cagccggctg ctcgattcgc tcgacgggcg gcagtttctc gccgcgacct tcggccagac
     1261 ctattacttc cggacaccgc gcgtcgtcct gcccggcgag acgccgctga cgggcaggca
     1321 ttccgacatg gtggcgaccg tggcgctgac cgccttccag cactggggcg cggatctcgg
     1381 cgtgcagtgg gacccgcaga acgagcgcag cgagcgcacc gaggtgaacc tgcagtacaa
     1441 gcccgccggc gatgcggtgg tcaacctcag ctaccgttac gagcgcttcg agcagctgcc
     1501 cgatatcatc cagggcgtca cgctgctgtg cagcccgctg ctcagcccgg cggggtgcaa
     1561 ccagcagggc tacgaccagg tggatttctc gtcggcctgg ccgatccggc gcaactgggc
     1621 ggtgttcgcg cgcgatgtgt acgacctgcg ccacggcgaa cagctggagg agttcgccgg
     1681 cttcgagtac cgtgcctgct gctggcggct gcgtctcggc gcccggcgct acgtgagcag
     1741 cttcaccggc gcgcgggaca ccggggtgtg gctgcagctg gaacttgcgg gcctcgccgg
     1801 tgtcggatcg gcgtcggata cctccctctc cgaggagatc cgcggctaca ctccccccga
     1861 ggcgaatacc cgcagaatca ggggcccgtg agaacgatgg tggacagcag gactcatccc
     1921 atgcggcgca tcctgacccc ggcctccctc gccctggtac tcggcgcagt ggccctggcc
     1981 gcgtacaccc cctcccgggc gcagacccgc gagctcgaga cccgcggggt gctgctcgac
     2041 cgcatcgccg ccatcgtcaa cgacggcgtg gtgctcacga gcgagctcga ccagcaggtc
     2101 gcggcggtga gcgagcggct gcgcacgcag aagctcgagc tccccccgca gaacgtgctg
     2161 cgccagcagg tgctcgaccg gctggtgctg caggagatcc agatgcagcg cgccaaccac
     2221 gacggcatca aggtctccga cgagacgctc aacgccgcgc tccaggaggt cgccaagcgc
     2281 aacaacctca ccctgtcgca gctgccggac gcgctcgccg cccagggcat cgactacgcc
     2341 gcctaccgcg acgacctgcg ccgggagatc acgctcggga tgctgcgcca gcgcgacgtg
     2401 ctcgaccata tcagcgtgac gccccgggag atcgaccagt acctggagaa gcaggcccgc
     2461 atgcccgcgc agaacagcga gtacaacgtg tcgcacatcc tgatcgcggt gccccaggag
     2521 gccaccccgg cgcagatcga ccaggccgcc cggcgcgccg cggaggtc
//
LOCUS       DATDMP010000232         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1257706, whole genome shotgun sequence.
ACCESSION   DATDMP010000232 DATDMP010000000
VERSION     DATDMP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257706"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VI195_03940"
     CDS             <1..974
                     /locus_tag="VI195_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY6823563.1"
                     /translation="ADATLLNEANGGIWDKEADLRREFWRADAAERRRALLPFLWSTV
                     AVRGQIFGNQTVGSVARVTNGLAFSYPGYNEMLTGHPDVRIDSNEFGPNPNISVLEWL
                     NTLPELHGKVTAYATWAAFKDIFNVPRSHLPLQVGWDLPYHGSLTPRQELMNHLYESA
                     TRFDKEDLYDAFLQVPLLDSFAKQQPRVLFVGYGETDNWAHAGRYDLLLHSAHSFDGY
                     VRELWERLQALPAYRDSTTFIITTDHGRGSGPVDWKEHGVEQKGSENIWIAVLGPDTA
                     ALGERRNTAEVHQAQIAATVAAFLGKDYRQAVPAAAPPISEVLGRAR"
     gene            complement(997..1785)
                     /locus_tag="VI195_03945"
     CDS             complement(997..1785)
                     /locus_tag="VI195_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY6823564.1"
                     /translation="MASSVNVVRVDLTRTLPRPEAKRSGNGLSLACCPARAALTTAEA
                     QPPGVGLDLAQVSILDGDHTVAQTMARVLQTEGFSARAFLSAPEFMAQPEPALAGCLV
                     ADASTPGRAGLELLRELTARQSLLPVILLIAPGDTRTVVKGMKAGAINCLPKPVQRAE
                     LLEAVREALAESRAVRAQRAAQLRVRQMLERLTPREREVLDLALDGLLVKQIAARLGT
                     AEKTIKTHKGRLMRKMEVRSTLALVQLMVNAGIPPVASAGQPSA"
     gene            complement(1881..>2133)
                     /locus_tag="VI195_03950"
     CDS             complement(1881..>2133)
                     /locus_tag="VI195_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY6823565.1"
                     /translation="GTWNVFKFVDPKTRLDLGLYVFPSLTESGRYRSTGNLNLTHKFP
                     HDITLGLTGYLSYDNQPPEPTAEKSDYGVTLNVGYTFGQ"
BASE COUNT          363 a          689 c          701 g          380 t
ORIGIN      
        1 gcgcggatgc gacgctgctg aacgaggcga acggaggcat ctgggacaag gaggcggatc
       61 tgcggcgcga gttctggcgt gcggacgccg ctgagcggcg ccgcgcgctg ctgcctttcc
      121 tgtggagcac ggtcgcggtg cgcgggcaga ttttcggcaa ccagacagtc ggcagcgtgg
      181 cgcgcgtcac gaatgggctg gcgttctcct accctggata caacgaaatg ctgacgggcc
      241 atcccgatgt gcgcatcgac tcgaatgagt tcggtcccaa tccgaacatc tcggtgctgg
      301 aatggcttaa tacactgccg gagcttcacg gcaaggtaac tgcctacgcc acctgggcgg
      361 ctttcaagga catcttcaac gtgccacgca gccatctgcc gctgcaggtc gggtgggacc
      421 tgccctacca cggttcactc acgccgcggc aggagttgat gaatcacctc tacgagtcgg
      481 cgacccgttt cgacaaggag gatctctacg acgcgttcct gcaggtgccg ctgctcgact
      541 ccttcgccaa gcagcagccg cgcgtgttgt tcgttggata cggcgagacc gacaactggg
      601 cccatgcggg ccgctatgac ctcctgctcc acagtgccca cagtttcgat ggctacgtcc
      661 gcgagctgtg ggagcggctg caggcgcttc cggcctaccg cgacagcacc accttcatca
      721 tcaccaccga tcacggccgc ggcagcggac cagtggactg gaaagagcat ggggtggagc
      781 agaaagggtc cgagaacata tggattgcag tgctcggccc ggataccgct gcgctcggtg
      841 agcgccggaa cactgcggaa gtgcatcagg cgcagattgc agcgaccgtg gctgcctttc
      901 tcggcaagga ctatcgccag gcggtgccgg ccgccgcgcc gccgatcagt gaggtgctcg
      961 gacgggctcg gtgagctgaa cgctcgagcg tgccctttac gcgctcggct gacccgccga
     1021 ggccaccggc gggatccccg cattgaccat cagctgcacc agcgcgagcg tcgagcgcac
     1081 ttccatcttc cgcatcagcc gtcccttgtg cgtcttgatg gtcttttccg cggtgccgag
     1141 gcgcgccgcg atctgcttga cgagcagccc atcgagtgcc agatcgagca cctcgcgctc
     1201 gcgcggagtc agccgctcga gcatctgccg cacacgcaac tgagctgcgc gctgtgcacg
     1261 caccgccctg ctctcggcca acgcttcccg caccgcctcg agcaactctg cacgctgcac
     1321 cggtttggga aggcaattga tggcgcccgc cttcatgcct ttgaccacgg tgcgggtgtc
     1381 gcccggggcg atgagcagaa tgacgggaag cagcgattgc cgggcggtga gttcccgtag
     1441 cagctcgagg ccggctcggc cgggcgtgct ggcatccgca acgaggcatc ccgcgagggc
     1501 gggctcgggc tgcgccatga actccggcgc cgacaaaaag gcgcgggcac tgaagccttc
     1561 cgtctggagc acccgcgcca tcgtctgggc aacggtgtga tcgccgtcga gtatcgacac
     1621 ctgtgccaga tccagaccga caccgggcgg ttgcgcctcg gccgtagtga gcgcggcccg
     1681 cgccgggcag caggccaaag acaggccatt gccgctgcgc ttggcctccg gccgtgggag
     1741 tgtcctggtc aggtcgaccc gtacgacgtt tacgctgctc gccacttctt ctccggtgca
     1801 attcgccggc tggcggacac gctaacaagc acacccgtgt ccgcctactg ccctttgggg
     1861 cagccggtag gttccaagag ctattgcccg aacgtatagc ccacgttgag ggtcacgccg
     1921 tagtcgctct tttccgctgt aggctcaggc ggctggttgt cgtagctcag gtaaccggtg
     1981 agcccgagcg tgatgtcatg gggaaacttg tgggtgagat tgaggttacc ggtgctgcgg
     2041 tagcggccgc tctcggtcag gctcgggaac acatagagcc caaggtcgag ccgcgttttc
     2101 ggatcgacga acttgaagac attccaggtg ccg
//
LOCUS       DATDMP010000233         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1812977, whole genome shotgun sequence.
ACCESSION   DATDMP010000233 DATDMP010000000
VERSION     DATDMP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812977"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VI195_03955"
     CDS             <1..253
                     /locus_tag="VI195_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6823566.1"
                     /translation="DDVKVTVADGMLTVSGERRQKEEENGEKFHKVETWYGNFSRSFA
                     LPDAVNEAAIRAESKDGVLTIHVPKTKVETKKPTNIKVQ"
     gene            358..1785
                     /gene="hemN"
                     /locus_tag="VI195_03960"
     CDS             358..1785
                     /gene="hemN"
                     /locus_tag="VI195_03960"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY6823567.1"
                     /translation="MHALPAPDSDNFPVIDTGLLERYDRPGPRYTSYPAAPQFTSAFG
                     TADLCGHATRSNLQPGPRPLSLYVHVPFCFSPCFYCGCNRLITRDTAKGERYVEWLLR
                     EIGLIAPLFRRGREVVQLHLGGGTPNFLSAATLEQLIDGLAAAFTLSRAPGRDYSIEL
                     DPRSVPPDYAAELARLGFNRVSLGVQDFDAEVQRAVNRMQSTEETLDLIDACRDSGFR
                     SVNVDLIYGLPRQTPTGFRRTLRMVMSARPDRLAVYGYAHLPGLFKAQRRIDSAMLPS
                     AAERLELLRLAIEELCAGGYRYIGMDHFALPDDELVQAQEAGTLQRNFMGYTTHAGCD
                     LIGLGVSAISHIGESYSQNFRELKKWESALAAGRLPLWRGLTLSADDRVRAEVIGQLM
                     CQGVIDMRAIEARYGLDFAAYFEGALARLQPHIADGLIVIADGRITATGSGRLLLRSI
                     AMCFDAYLSAPSAAGASRPSFSRVV"
     gene            complement(1804..>3648)
                     /locus_tag="VI195_03965"
     CDS             complement(1804..>3648)
                     /locus_tag="VI195_03965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6823568.1"
                     /translation="RQLFEAAQILSQYRTYMGPRLAIVSNGGGPAVLATDALIEGGGE
                     LAPLGSQTLEALNGVLPATWSHANPIDIIGDAPPERYVGALSAVLEDPQVDAVLLVHA
                     PTAVAPTAAVARVCTPLLEKATRPAFACWLGAEGVQLAGAAGPGAVPGYATPEEAVEA
                     YLQVMRYHQVQALLMQSPTSQPEGPAPDTASARSIVASALAAGRFVLSEPESKELLAA
                     YHIPIVETRIGRDIAALAALAEDIGYPVALKILSPDVSHKSDVGGVALDIVGAAELAQ
                     AAETMLQRCRTLRPQARIEGFTVQRMIRRGGAHELIAGIALDPTFGPVVLFGRGGTAV
                     EVIGDRALALPPLNSVLTRELIARTRVARLLAGARGVRPVALPALEQVLMRLAQLACD
                     LGGIVELDINPLLADEEGVIALDARVRLAPSAASGVARLAIRPYPSELEETLDYRGRQ
                     LLLRPIRPEDTPRHRQFLRQVTPRDLYLRFFSGVRELPEADLAHLTQIDYDREMAFVA
                     VGRDAAGEEEILGVARACSDPDNHTAEFAVLVRSDLKGQGLGTLLMHKLIRYCRERGT
                     GELWGTVMAENSAMLQLARSLGFKVRGTEQNIETIALQLQPPPAARAV"
BASE COUNT          606 a         1244 c         1231 g          567 t
ORIGIN      
        1 ggacgacgtc aaggtcacag tcgccgacgg catgttgacc gtgagcggtg agcggcgcca
       61 gaaggaagag gagaacggcg agaaattcca caaggtggag acctggtacg ggaatttctc
      121 acgcagtttc gcgctaccgg atgcggtcaa tgaggccgcg atccgcgccg agtcgaagga
      181 cggggtgctc accatccacg tcccgaagac caaggtcgag acgaagaagc caacaaacat
      241 aaaggttcag taggccaagc ggctggcgca ccggccgcgg cgatgacggt aggatggcat
      301 cgtcatcgcc gcggcgaatt tgctcctggc gacggccgtg caacgcggga atcgactatg
      361 cacgccctgc ctgcaccgga ttccgacaac tttccggtta tcgacaccgg gctgcttgag
      421 cgctacgacc ggccgggccc gcgctacacc tcgtaccccg cagccccgca gttcacgagc
      481 gccttcggta ccgcggatct gtgcgggcac gcgacgcgca gcaacttgca gcccggcccg
      541 cggccgctgt cgctgtacgt gcacgtgccc ttctgcttca gcccctgctt ctattgcggc
      601 tgcaaccgcc tcatcacgcg cgacaccgcg aagggtgaac gctacgtcga gtggctgctg
      661 cgcgagatcg ggctgatcgc accgctcttc cggcgtgggc gggaggtcgt gcaactgcac
      721 ctgggcggcg gcacacctaa ttttctcagc gccgcgactc tcgagcagct catcgacggc
      781 ctcgccgcgg ccttcaccct gagtcgcgct cccggccgcg actactcgat cgagctcgac
      841 ccgcgcagcg tgccgccgga ctatgcggcg gagctggcgc ggctcggatt caatcgcgtc
      901 agcctcgggg tgcaggactt cgacgccgag gtgcagcggg cggtcaaccg catgcagagc
      961 accgaggaga cgctcgatct catcgatgcc tgccgcgaca gcggctttcg ctcggtgaac
     1021 gtcgatctga tctacgggct cccgcgccag acgcccaccg gcttccgccg gacgctgcgc
     1081 atggtgatgt cggcacgccc cgaccgtttg gcggtctacg gttacgcgca cctgccgggc
     1141 ctcttcaagg cgcaacgccg gatcgattcg gccatgctgc cgagcgccgc cgagcggctc
     1201 gagctgctgc ggcttgcgat cgaagagctc tgcgccggcg gctaccgcta catcggcatg
     1261 gaccatttcg cgctgcccga tgatgagctg gtgcaagcgc aggaagcagg cacgctacag
     1321 cgtaatttca tgggctacac gacacacgcc ggctgcgacc tcatcggtct cggagtgagc
     1381 gccatcagtc acatcggcga gagctacagc cagaacttcc gcgagctgaa gaaatgggag
     1441 agcgcgctcg ccgccggccg cctgccgctg tggcgcggac tgacgctgag cgcagatgac
     1501 agggtccggg cggaggtgat cgggcagctc atgtgccagg gcgttatcga catgcgtgcg
     1561 atcgaggcac gctacggcct cgatttcgct gcgtacttcg agggcgcact cgcacggctg
     1621 caaccgcaca tcgcggacgg tctcatcgtg atcgccgatg gacgaatcac cgcgaccggc
     1681 agcggacggt tgctgctgcg gagcattgcg atgtgcttcg atgcctacct gagcgcaccg
     1741 tccgcagcgg gtgcgtcgcg cccgtccttc tcgcgcgtcg tctgagaagc ggtagcggct
     1801 gcctcagacg gcacgcgcag cgggcggtgg ctgcagttgg agggcgatgg tctcgatgtt
     1861 ctgctcggtg ccgcgcacct tgaagccaag gctcctggcg agctgcagca tggcgctgtt
     1921 ctcggccatg acagtgcccc agagctcacc ggtgccgcgc tcgcggcaat agcggatcaa
     1981 cttgtgcatg aggagcgtcc cgagcccctg gcccttgagg tccgagcgta ccagcaccgc
     2041 gaattccgca gtgtggttat cgggatcgga gcaggcacgc gcgaccccga ggatttcttc
     2101 ctcgcccgcc gcatcgcggc ccaccgccac gaaagccatc tcgcggtcgt agtcgatttg
     2161 cgtgaggtgt gcgagatccg cctccggcag ctcgcgcacg ccgctgaaaa agcgcaggta
     2221 cagatcacgc ggcgttacct gccggagaaa ctgccggtgc cgcggggtgt cttccggtcg
     2281 gatcggccgc agcagcagct ggcggccgcg gtaatcgagc gtctcctcga gctcgctcgg
     2341 gtaagggcgg atcgcaagcc gtgcgacccc gctcgccgca ctcggggcaa ggcgcacccg
     2401 cgcatcgagc gcgatcaccc cttcctcatc cgcgagcagc ggattgatgt cgagctcgac
     2461 gatccccccc aggtcgcacg cgagctgcgc gagccgcatc agcacctgct cgagcgccgg
     2521 cagcgcgacc ggccgcacgc cgcgggcacc ggcgagcagc cgcgccaccc gggtgcgcgc
     2581 gatgagctca cgggtgagga cggagttgag tggcgggagc gcgagcgcgc gatcgccgat
     2641 gacttcgacc gccgtgcccc cgcggccgaa aagcaccaca ggtccgaagg tcggatcgag
     2701 cgcgatgccc gcgatgagct cgtgcgcacc accgcggcgg atcatgcgct gcaccgtgaa
     2761 accttcgatg cgcgcctgcg gccgcagcgt ccggcaacgc tgcagcatcg tctcggccgc
     2821 ctgcgcgagc tcggcggcgc ccacaatatc gagtgcgaca cccccgacgt cggatttgtg
     2881 gctgacatcg ggcgaaagga tcttgagggc aaccgggtag ccgatgtcct cggcgagcgc
     2941 tgcgagcgcg gcaatatcgc ggccgatgcg ggtctccact atgggaatgt gataggccgc
     3001 cagcagctct ttcgactccg gctcactcag aacgaagcgg cccgcggcga gcgcggaggc
     3061 gacgatcgat ctcgcgctcg ccgtgtcggg tgcgggtccc tcgggctgcg aggtgggact
     3121 ctgcatgagc agcgcctgca cctggtgata gcgcatgacc tgcaggtacg cctcgacggc
     3181 ttcttcgggc gtggcgtaac ccggtaccgc gcccggcccc gccgcgcccg cgagctgcac
     3241 cccttccgct ccgagccagc aggcgaaggc gggccgtgtc gccttctcga gcagcggtgt
     3301 acagacgcgc gccaccgctg cggtgggcgc caccgcagtc ggtgcgtgca cgagcagtac
     3361 cgcatcgacc tgcggatcct cgagtaccgc actcaacgca ccgacatagc gctcgggcgg
     3421 agcatccccg atgatgtcga tcggattggc atgcgaccac gtggccggca gcaccccgtt
     3481 gagtgcctcg agcgtctgcg agccaagcgg cgcgagctcc ccgcccccct cgatgagcgc
     3541 atcggtggcc agcacggccg gtccgccgcc gttactgacg atggcgaggc gcggacccat
     3601 gtaggtgcgg tactgcgaga gaatctgtgc cgcctcgaag agctgccg
//
LOCUS       DATDMP010000234         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1702302, whole genome shotgun sequence.
ACCESSION   DATDMP010000234 DATDMP010000000
VERSION     DATDMP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702302"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1303)
                     /locus_tag="VI195_03970"
     CDS             complement(47..1303)
                     /locus_tag="VI195_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6823569.1"
                     /translation="MLVRFFFELRASGVPVSITEFLTLLTALAARLAALSAQDFYYLA
                     RACLVKDERHYDRFDRVFAQVFEGAEQVFAQLSAQVPAAWLQALAARVLSAEERRLVE
                     SLGGWDKLMEALRQRLREQRERHAGGNRWIGSGGTSPFGAGGYNPLGVRIGQTGAGQK
                     RAVKVWERREYRNLDDTVELGTRNLKLALRRLRRFAREGAPEELDLEGTIDATARNGG
                     LLDLRLVPQRHNTVKVLLFLDVGGSMDTHVRICEELFSAARSEFKHLEYFYFHNCLYE
                     SVWKDNQRRFTGLIATERVLRTYGRDYRVILVGDASLSPHEIMQPGGSIEHWNEEAGA
                     AWMRRLCAAFPRLVWLNPEDPARWAYTPSTSILTELIQGRMFPLSLAGLDLAIGELRR
                     PLSGAGAASYGAGATLVTQSPPPQVP"
     gene            complement(1314..2168)
                     /locus_tag="VI195_03975"
     CDS             complement(1314..2168)
                     /locus_tag="VI195_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002049937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6823570.1"
                     /translation="MNEPQRFTGTDRYVATDDLMMAVNAAITLARPLLIKGEPGTGKT
                     QLAQEIARSLGRPLYEWHIKSTSKAQQGLYEYDAVSRLRDSQLGEERVHDIGNYIVKG
                     RLWEAFESQVQPVLLIDEIDKADIEFPNDLLRELDRMEFYVHETRQLVRARHRPIILI
                     TSNNEKELPDAFLRRCFFHYIRFPDETTMRAIVGVHYPELKKELLSEALSAFFEVRAT
                     PGLKKKPSTSELLDWIKLLVAEDIPPEALRSGEARKVIPILHGALLKNEQDVHLFEQL
                     VFIHRRAR"
     gene            2292..>3390
                     /locus_tag="VI195_03980"
     CDS             2292..>3390
                     /locus_tag="VI195_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6823571.1"
                     /translation="MAPADPAELIQRAVAAARRQAWGEAAELARSVLERCGPEANALM
                     VLASVRIHAGDLPGAIALYEQARALMPQHIHVLVNLAALYRSAGRLGEARDTIECALR
                     VDAGFAVAHNNFGNILGDLGERAESLRAYESAAALDPNYADPLAAMARIAADEHRLDD
                     ARVLAQRALQLAPQNVLATLTLARVKLRQEDAAAAATILESLLREPTLSATNRILAQG
                     YLGEAYDRVARYRDAFRAFSEANALQQRQYAGMSQLERGPLAPASIARLTAFMLGTDL
                     ARWRQAPPVASCPVFLLGFPRSGTTLLEQMLASHPDVTSLEERDTLVDAAGELLGTAP
                     PFDGWSELADREIERLRERYWQRVTSGLPQPP"
BASE COUNT          581 a         1130 c         1110 g          569 t
ORIGIN      
        1 gaaggtttcg gcgagcccat ccataatgtt cgtgtcagcg ccgctattat ggaacctggg
       61 gtggcgggct ctgtgtgacc aaagtcgcgc ccgcaccgta ggaagcggcc ccggcaccgc
      121 tcaaggggcg gcgcagctcg ccgatggcga ggtcgagtcc cgcaaggctc aaggggaaca
      181 tccgcccctg gatgagctcg gtcaggatgc tggtggaggg cgtgtaggcc cagcgtgcgg
      241 gatcttccgg attcaaccac accaggcgcg ggaaagccgc acacaggcgt cgcatccatg
      301 ccgcgcccgc ttcctcgttc cagtgctcga tgctgccgcc gggttgcatg atctcatgag
      361 gactcaggct ggcatcgccg accaggatga ccctgtagtc acggccgtaa gtgcgcagca
      421 cccgctcggt ggctatcaac cccgtgaagc gccgctggtt gtccttccat acactctcgt
      481 atagacagtt gtgaaagtag aaatactcca ggtgcttgaa ctcgctgcgc gcggccgaga
      541 acagctcctc gcagatgcgc acgtgagtgt ccatcgaccc gccgacatca agaaaaagaa
      601 gaaccttgac ggtgttatgc cgctgcggca cgaggcgcag atcgagcaga ccgccgttgc
      661 gcgcggtcgc gtcgatcgtg ccctcgaggt cgagctcctc gggcgcgccc tcgcgcgcga
      721 agcggcgcag ccggcgcagc gccagcttca ggttgcgcgt gccaagctcg accgtgtcgt
      781 cgaggttgcg gtactcgcgc cgctcccaga ccttgaccgc gcgcttctgg ccagcgcccg
      841 tttgccctat gcggacacca agcgggttgt agccgccggc gccgaagggt gaggtaccgc
      901 cgctgccgat ccagcggttg ccgccggcgt ggcgctcgcg ctgctcgcgc agtctctggc
      961 gcagggcttc catgagcttg tcccagccgc cgagggactc cacaagacgc cgctcctcgg
     1021 cggagagaac gcgtgcagcc aacgcctgca gccaggcggc cggcacctga gccgacagtt
     1081 gcgcgaacac ctgctccgcg ccttcgaaca cctgcgcgaa cacccggtcg aagcggtcgt
     1141 agtgacgctc gtccttcacc aggcaggcgc gcgcgaggta gtagaagtcc tgcgcggaga
     1201 gcgcggcgag ccgtgccgcg agcgccgtga gcagcgtcag gaactcggtg atcgagaccg
     1261 gcacaccgct ggcgcgcagc tcgaagaaaa atcgcaccaa caccgcagca ccttcagcgg
     1321 gcgcggcggt ggatgaatac cagttgctcg aacaggtgca catcctgctc gttcttgagc
     1381 agcgcgccgt gcaggatggg aatgaccttg cgcgcctccc cgctgcgcag cgcctcgggc
     1441 gggatatcct ccgccaccag cagcttgatc cagtcgagca gctcggaagt cgagggcttt
     1501 ttcttcagcc cgggcgtggc acgcacctcg aagaacgcag acagcgcctc gctcagcaac
     1561 tccttcttca gctcggggta atgcacgccg acgatggcgc gcatggtggt ctcgtcgggg
     1621 aagcggatgt agtggaagaa acagcgacgc aagaacgcgt ccggcagctc cttctcgttg
     1681 ttgctggtga tgaggatgat cggccggtgc cgtgcgcgca ccagctggcg cgtctcgtgc
     1741 acgtagaact ccatgcgatc gagctcgcgc agcaaatcgt tgggaaactc gatgtcagcc
     1801 ttgtcgatct catcgatcag cagcaccggc tgcacctgcg actcgaaagc ctcccacagc
     1861 cggcccttga cgatgtagtt gccgatgtca tggacgcgct cctcaccgag ctgcgaatcg
     1921 cgcagccggg aaacggcgtc gtactcgtac agaccctgct gcgccttgct ggtggacttg
     1981 atgtgccatt cgtacagcgg ccgtccgagg gaacgcgcga tctcctgggc cagctgggtc
     2041 ttgccggtgc ccggctcgcc cttgatgagc agcggtcgcg ccagggtgat ggcggcgttc
     2101 accgccatca tgagatcgtc ggtggcgaca taccggtcgg tgcccgtgaa gcgttgcggc
     2161 tcgttcatgg cagtcagtct aaccgcacgc gcctgaatgg tcgcggaatc ctcatctgtt
     2221 ccccaacacg cgccagtcgg cagatgagga tataaagtca acttccgcaa ggctgataga
     2281 atggagaaat cgtggcgcca gcggatcccg ccgaactcat ccagcgcgcc gtggccgccg
     2341 cgcggcgcca ggcctggggt gaagctgcag agttggcgcg ctcggtcctc gagcgctgcg
     2401 gtcccgaagc gaacgccctg atggtgctcg cgagcgtgcg catccacgca ggtgatctgc
     2461 cgggggccat tgctctttat gagcaggcac gcgcgctcat gccgcagcac attcacgtac
     2521 tggtgaatct agccgctcta taccgctcgg ccggcaggct cggcgaagcg cgcgacacaa
     2581 tcgagtgcgc gctgcgggta gacgcgggct ttgcggtagc tcacaacaat ttcggcaaca
     2641 tactcggaga cctcggcgag cgcgccgagt cgttgcgtgc ctacgaaagt gccgccgcgc
     2701 tcgacccgaa ttatgccgat ccgctagcgg ccatggcacg cattgcggca gatgagcacc
     2761 gcctggatga tgcccgggtc ctcgcccaac gcgcgctgca gctggcgccg cagaatgtgc
     2821 tggcgacact gacgctcgcg cgcgtcaagc tgcgccagga ggatgcggcc gcagccgcta
     2881 ccatcctcga gtcgctgctg cgcgagccca ccttgtcggc gacgaatcgt atccttgccc
     2941 agggttacct gggagaagcg tacgacaggg ttgcccgcta ccgcgacgcg ttccgggcct
     3001 tcagcgaggc caatgcgctg cagcagcggc aatatgccgg catgtcgcag ctcgagcgcg
     3061 gtccgcttgc ccccgcgagc atcgctcggc tgacggcgtt catgctgggc accgatctcg
     3121 cgaggtggcg gcaggcgccc ccggtagcgt cttgcccggt gttcctgctc gggttccccc
     3181 gctccggcac gaccctgctc gagcagatgc tcgcctcgca tcccgatgtc accagcctcg
     3241 aggagcgcga caccctcgtc gacgccgccg gcgagctgct gggcaccgcg ccgccgttcg
     3301 atggctggtc ggagctcgcc gatcgcgaga tcgagcggct gcgcgagcgg tactggcaac
     3361 gggtgacgag cggcctccca cagccaccta
//
LOCUS       DATDMP010000235         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1035396, whole genome shotgun sequence.
ACCESSION   DATDMP010000235 DATDMP010000000
VERSION     DATDMP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035396"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..792
                     /gene="lptA"
                     /locus_tag="VI195_03985"
     CDS             127..792
                     /gene="lptA"
                     /locus_tag="VI195_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY6823572.1"
                     /translation="MARSCPKLVPLLLGAALPAVTAAAPEPPAQQPISVDAASSEVDY
                     KTNTVAFRNVVISQGNTRVQADHAQAAGLNFANSRWNLEGHVHIEAEQHGRLSSDQAT
                     VEFRDNHIARATVTGKPAEFEQQRAGSDQIARGHADQIEYDVSDGTVRLADDAWVTDG
                     QNEISGPVLVYNIREQRVQAAAAPGSDQRVHISIAPRALETGKLDAPKKPPPKPPGPG
                     PQP"
     gene            789..1514
                     /gene="lptB"
                     /locus_tag="VI195_03990"
     CDS             789..1514
                     /gene="lptB"
                     /locus_tag="VI195_03990"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY6823573.1"
                     /translation="MTALRATGLAKSYKSRPVVRDLSLELESGEVVGLLGPNGAGKTT
                     AFYMIVGLVPCDAGRIHLGDRDVTLLPMHRRAQLGLGYLPQEASVFRKLSVEDNVLAI
                     LETRPDLERAARQERLEQLLEELRIGHVRRTLGLSLSGGERRRVEIARALAAEPRFML
                     LDEPFAGVDPLSVLDIQRIIRELTHRGIGVLITDHNVRETLGVCSRAYIVNQGTVIAS
                     GTAEEVLANPQVREVYLGESFRL"
     gene            1613..>2128
                     /locus_tag="VI195_03995"
     CDS             1613..>2128
                     /locus_tag="VI195_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823574.1"
                     /translation="MLKPSLQLKLGQQLTMTPQLQQAIRLLQLPALELQAHIRSLLET
                     NVMLEPVEESEATGTFEAPVSAAVTPGTEGPRQDAVEVLDEPWAQQNVGPADSPWSGE
                     DEERQQEYADSSGESLQDHLLWQLELARLAPRELAVARALVDAISDDGYLTESLEEIA
                     RTLRPELECTPE"
BASE COUNT          362 a          752 c          710 g          304 t
ORIGIN      
        1 acacccgtac gcagatcgcc tccacgcgcg acccggtcac actcgtcatg tcgggccgga
       61 ccttgagcgc gcaggggctg gtggcgagct tgaaggaggg tcgcgtgcag ctagaatcgg
      121 ccgtgcatgg ctcgttcctg ccctaagctc gtgccgctgc tgctcggcgc cgcgctgccg
      181 gccgtcacgg cggctgcgcc ggagcccccc gcccagcagc cgatctcggt cgatgccgct
      241 tcctcggagg tggattacaa gaccaatacc gtagccttcc gcaacgtcgt catctcccag
      301 ggcaacacgc gggtgcaggc ggaccatgcg caggccgcgg gactcaactt cgccaacagc
      361 cgctggaacc tcgagggtca cgtgcacatc gaggccgagc agcacgggcg cctcagctcc
      421 gatcaggcga ccgtcgaatt ccgcgacaat cacatcgcgc gcgcgaccgt caccggcaag
      481 ccggccgagt tcgagcagca gcgtgccggc tcggatcaga ttgcgcgcgg ccacgccgac
      541 cagatcgaat acgacgtgag cgacggcacc gtgcggctcg cggacgatgc ctgggtcacc
      601 gacgggcaga acgagatctc cggcccggtg ctcgtgtaca acatccgcga gcagcgggtg
      661 caggccgccg ccgcaccggg cagcgaccag cgcgtgcaca tcagcatcgc gccgcgcgcg
      721 ctggagacgg gcaagctcga cgcgccgaag aagccgccac cgaagccgcc cggccccggc
      781 ccgcagccat gaccgctctc agggcgaccg gtctcgccaa gagttacaaa tcccgcccgg
      841 tggtccgcga tctgtcgctc gagctcgaga gcggcgaggt ggtgggtctg ctcggaccga
      901 acggcgcggg caagaccacg gccttctaca tgatcgtcgg tctggtgccg tgcgacgccg
      961 gccgcatcca cctcggcgat cgcgacgtga cgctgctgcc gatgcatcgg cgcgcgcagc
     1021 tcgggctcgg ctacctgccc caggaggcat cggtcttccg caagctctcg gtggaagaca
     1081 atgtgctcgc gatcctcgag acgcggcccg atctcgagcg cgccgcgcgc caggagcgtc
     1141 tcgagcagct gctcgaggag ctgcgcatcg ggcacgtgcg ccgcacactc ggcctgtcgc
     1201 tctccggcgg cgagcggcgc cgggtggaga tcgcgcgggc gctcgccgcg gagccgcgct
     1261 tcatgctgct cgacgagccc ttcgccggtg tcgatccgct gtcggtgctc gatatccagc
     1321 gcatcatccg cgagctcacc caccgcggca tcggcgtgct gatcactgac cataatgtcc
     1381 gcgagacgct cggcgtctgc agccgcgcct acatcgtcaa ccagggcacg gtcatcgcgt
     1441 ccggaactgc agaagaagtc cttgccaacc cccaagtccg tgaggtgtac ttgggcgagt
     1501 cgttccggct atgaagctcc cggggggaca cggcaccgag ccacaagcgc ttatctttca
     1561 attaattacc gagtgaaatc cgcggggccg aagcggtccc cgatacgcca ccatgctgaa
     1621 accctcactg cagctgaagc tgggtcaaca actgacgatg accccgcagc tgcagcaggc
     1681 gatccggctg ctgcagctcc cggcactcga gctgcaggcg cacatccgct cgctgctcga
     1741 gaccaacgtg atgctcgagc ccgtcgagga gagtgaagcc accggcacgt tcgaggctcc
     1801 ggtgagcgcc gccgtcaccc cgggcaccga gggcccgcgg caggatgcgg tcgaggtcct
     1861 cgatgagccc tgggctcagc agaatgtagg cccggccgac agtccctgga gcggcgagga
     1921 cgaggagcgc cagcaggagt acgccgattc gagcggcgag tcgctgcagg atcatctgct
     1981 ctggcagctc gagctggcac ggctcgcgcc gcgcgagctc gccgtggccc gtgcgctggt
     2041 ggacgccatc agcgacgacg gctatctcac cgagtcgctg gaggagatcg cccggacgct
     2101 caggcccgag ctcgagtgca caccggag
//
LOCUS       DATDMP010000236         3358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1813431, whole genome shotgun sequence.
ACCESSION   DATDMP010000236 DATDMP010000000
VERSION     DATDMP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813431"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VI195_04000"
     CDS             complement(<1..1113)
                     /locus_tag="VI195_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6823575.1"
                     /translation="MERPWLKSYPAGIATEIDPNEFSSLVDLFEKSIRQYAERPAFHC
                     MGRTLSFGELDRRSREFGAWLQGKGLGKGGRVAIMLPNCLQYPIAMFGTLRAGCAVVN
                     VNPLYTARELEHQLADSGAEVIVILENFAHVLQEVRARTSIRHVVLTSLGELLGAKGI
                     LVNLVVRKVKKLVPPFQLAGLQSFKQALAEGAGKRLQTPALTHDDLAFLQYTGGTTGI
                     SKGAMLLHRNVVAALLQYRAWLAPAMKGERPVIITALPLYHIFSLTVNCLNMMALGGE
                     NILITNPRDIPGFVKELGRHRYNIITGVNTLFNALLNHADFRKLDFSALKLAVGGGMA
                     VQKAVAERWKEVTGSTLVEGYGLTETSPSATANPPDL"
     gene            complement(1167..1943)
                     /locus_tag="VI195_04005"
     CDS             complement(1167..1943)
                     /locus_tag="VI195_04005"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY6823576.1"
                     /translation="MTYEFILTEKKDRVGLLTLNRPKQLNALNPKLMQELGHALQAFD
                     ADEGIGAIVITGSEKAFAAGADIGVMKDYSYMDAFMADYITRDWEHIRRIRKPIIAAV
                     AGYALGGGNELAMMCDIVIAADNARFGQPEINLGIMPGAGGTQRLPRFTSKAKAMDLC
                     LTARMMDAQEAERAGLVSRVVPAEKLMEEALAAASRIASCSLPVAMMIKESINRAYET
                     ALSEGVLFERRLFHSQFALEDQKEGMAAFIEKRKPTFKNR"
     gene            1981..2766
                     /gene="paaG"
                     /locus_tag="VI195_04010"
     CDS             1981..2766
                     /gene="paaG"
                     /locus_tag="VI195_04010"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEY6823577.1"
                     /translation="MSAVLVSVEKGVLVATLNRPDKLNACTPEMLALLREAIQRAADE
                     PGIRAVLLTGAGRGFCAGQDLSMRNPAAGTAPIDLSVSLGSNYNPLIRRMRELPKPIV
                     AAVNGVAAGAGANIALACDIVLAGRSASFVQAFSKIGLVPDSGGTYFLPRLVGTARAM
                     GLALLGERLSAEDAEHWGLIWKTVDDDDLLDEATDLARALAAGPTRGYGLLKKALYAF
                     PANTLDAQLDLERDLQREAGLTEDYREGVAAFLQKRAPQFKGR"
     gene            2774..>3358
                     /locus_tag="VI195_04015"
     CDS             2774..>3358
                     /locus_tag="VI195_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEY6823578.1"
                     /translation="MSAKAFELEPVEKCSEKELKRLQLERLRWSLKHAYDNVPHYRKK
                     FDAAGVRPEDCRALGDLARFPFTTKADLRDTYPFGMFAVPMDRIVRIHASSGTTGKPT
                     VVGYSAKDIDTWAQVMARSIRAAGARPGDKVHVAYGYGLFTGGLGAHYGAERLGLAVI
                     PLGGGMTERQVQLIRDFKPEIIMVTPSYMLAIADE"
BASE COUNT          561 a         1093 c         1100 g          604 t
ORIGIN      
        1 gaggtccggc gggttggcgg tcgccgaggg ggaggtctcg gtgagcccgt agccctcgac
       61 gagcgtgctg ccggtgacct ccttccaccg ctcggccacg gccttctgca ccgccattcc
      121 gcctccgacc gcgagcttaa gcgcggaaaa atccagcttg cggaaatcgg cgtggttgag
      181 aagcgcgttg aagagcgtgt tgaccccggt gatgatgttg tagcgatgcc tgccaagctc
      241 cttgacgaat ccagggatgt cgcgcgggtt cgtgatcagg atgttctcgc cgcccaacgc
      301 catcatgttg aggcagttca ccgtcagcga aaagatgtga tagagcggca acgcagtgat
      361 gatgaccggg cgctcgccct tcatcgccgg cgcaagccag gcgcggtact gcagcagcgc
      421 ggccaccaca ttgcgatgca ggagcatcgc gcccttcgag atgccggtgg tgccgcccgt
      481 gtattggaga aatgcaaggt catcgtgcgt tagcgccggg gtctggagcc tctttccggc
      541 gccttcggcg agggcctgct tgaagctttg tagcccggcg agctggaaag gcggcaccag
      601 cttcttgacc ttgcgcacca ccaggttgac gaggatgccc ttcgcgccga gaagctcgcc
      661 gagtgaagtg agcacgacgt gccggatcga agttcgcgcc cggacctcct gcagcacgtg
      721 cgcgaagttc tcgaggatca cgatgacttc ggcgcccgag tccgcgagct ggtgctcgag
      781 ctcgcgcgcc gtgtagagcg ggttgacgtt taccacggcg cagccggcgc gcagcgtgcc
      841 gaacatcgct atcgggtact gcaggcagtt gggcagcatg atggcgacgc ggccgccctt
      901 gccgaggcct ttgccctgca gccaggcgcc gaactcgcgc gagcggcggt cgagctcgcc
      961 gaagctgagg gtcctgccca tgcaatggaa cgccggccgc tcggcgtact ggcggatgct
     1021 cttctcgaag agatcgacga gcgacgagaa ctcgtttgga tcgatctcgg tcgcgatgcc
     1081 ggcgggatag ctcttcagcc aggggcgttc cacaggctcc tctcccagga atccaccggg
     1141 aattctacgc tgcgcgaaag agcgccctag cgattcttga aggtgggctt gcgcttctcg
     1201 atgaaagccg ccatgccttc cttctggtcc tcgagcgcga actgcgaatg gaagaggcgc
     1261 cgctcgaaca gcaccccctc ggagagcgca gtctcgtagg cgcggttgat cgactccttg
     1321 atcatcatcg ccaccggaag cgagcagctc gcgatgcgcg acgccgccgc aagcgcttcc
     1381 tccatgagtt tttccgccgg cacgacgcgc gacaccagcc cggcgcgctc cgcctcctgc
     1441 gcatccatca tgcgcgcggt caggcacagg tccatggcct tcgccttgct cgtgaagcgc
     1501 ggcagccgtt gcgtgccgcc cgcgcccggc atgatgccca ggttgatctc gggctgcccg
     1561 aaacgcgcgt tgtcggcggc gatgacgatg tcgcacatca tcgcgagctc attgccgccg
     1621 cccagggcgt agccggcaac ggccgcgatg atcggcttgc ggatgcgacg gatatgctcc
     1681 caatcgcggg tgatgtagtc cgccatgaag gcgtccatgt agctgtagtc cttcatcacg
     1741 ccgatgtccg caccggcggc gaaggccttc tcgctcccgg tgatgacgat ggcgccgatt
     1801 ccttcgtcgg catcgaacgc ctgcagcgcg tgcccgagct cctgcatgag cttggggttg
     1861 agcgcgttca gctgcttcgg cctgttgagg gtcagcaggc caacgcgatc tttcttctcg
     1921 gtgaggatga attcgtaggt catataccgg cccattctaa tcgtggtgta agctttttcg
     1981 atgtccgctg tgctggtttc ggtcgagaag ggcgtgctcg tcgccacgct caaccgcccc
     2041 gacaagttga atgcctgcac gcccgagatg ctcgcgctgt tgcgcgaggc gatccagcgc
     2101 gcggccgacg agccggggat ccgcgccgtg ctcctcaccg gcgcggggcg cggtttctgc
     2161 gcgggccagg atctttccat gcgtaatccc gcggcgggca ccgcccccat cgacctctcg
     2221 gtttcgctcg gctcgaacta caacccgctc atccgccgca tgcgcgagct cccgaaaccc
     2281 atcgtcgcgg cggtgaatgg cgtggccgca ggagccgggg ccaacatcgc gctcgcctgc
     2341 gacatcgtgc tggcgggccg gtccgcgagc ttcgtccagg ctttttccaa aatcggcctg
     2401 gtccccgatt cgggcgggac ctatttcctg ccgcgcctcg tggggaccgc tcgcgcaatg
     2461 gggctcgcgc ttctcggcga gcgtctttcg gcagaggacg ccgagcactg gggccttatc
     2521 tggaagacgg tggacgacga cgatctcctc gacgaagcga ccgacctcgc gcgcgcgctc
     2581 gccgcgggcc caacgcgcgg atacggcctg ctaaagaagg cgctttacgc cttccctgcc
     2641 aacacgctcg atgcgcagct cgacctcgag cgcgacctgc agcgcgaagc cggcctcacc
     2701 gaggactacc gcgagggcgt ggccgccttc ctgcagaaac gcgcgccgca attcaaggga
     2761 aggtgaggcg gaagtgagtg cgaaggcttt cgagctggag ccggttgaaa aatgctccga
     2821 aaaggagctg aagcgcttgc agctggaaag gctgcgctgg tcgctcaagc atgcgtacga
     2881 caatgttccg cactatcgga aaaaattcga cgccgccggg gtaaggcccg aagattgccg
     2941 ggcgcttggc gatctcgcgc gcttcccgtt cacgaccaag gccgacctgc gcgacaccta
     3001 tcccttcggc atgttcgccg tgccgatgga ccgaatcgtg cgcatccatg cgtcgtccgg
     3061 aaccaccgga aagcctacgg tggtgggcta tagcgcgaag gacatcgata cttgggcgca
     3121 ggtcatggcg cgctccatcc gcgccgccgg cgctcggccc ggagacaagg tgcacgtggc
     3181 gtacggctat ggccttttta ccggcggcct cggtgcccac tacggcgccg agcgcctcgg
     3241 gctcgcggtg attccgctcg gcggcggaat gaccgaacgg caggtgcagc tcatccggga
     3301 tttcaagccc gagatcatca tggtgacgcc gagctacatg ctcgccatcg ccgacgag
//
LOCUS       DATDMP010000237         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_36374, whole genome shotgun sequence.
ACCESSION   DATDMP010000237 DATDMP010000000
VERSION     DATDMP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36374"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VI195_04020"
     CDS             <1..185
                     /locus_tag="VI195_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6823579.1"
                     /translation="YRFEAEWQLACERMTAAGFAAVSSFNPYWNLRGRSFQDPDGYRV
                     VLQREAWAPGPEGGLV"
     gene            complement(196..>2217)
                     /locus_tag="VI195_04025"
     CDS             complement(196..>2217)
                     /locus_tag="VI195_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY6823580.1"
                     /translation="LNAWVRIGSDDSITILVDRSEMGQGVYTALPTLLAEELEIDPSQ
                     VKVVAAPVGEAYVNALNSGQITGTSNSIADAWEKLRKAGAQARSMLITAAARRWRVDP
                     SSCRASNGRITSASGRSASYGELADAAAKVPVPKEVPLKSPGEFRLIGKSLPRLDTPG
                     KVDGSAQFGLDVKLPGMLHAAIALCPELGGTVASVDSAAALAMPGVRRIVPTASAVIV
                     VAEHFWQAKKARDALRIVWAAGPNAQLDNAAIWAQLNAAAAKPGVSALASDNVAAALK
                     GGHAAEALKRAAKTFAAVYELPLLAHAPMEPMNCTADVRANSCDLYVGTQAQQLAQAA
                     AAEAAGLKANQVNVHTTLLGGGFGRRLDVDFVPAAVAASKAVGAPVKLIWTREDDMSH
                     DLYRPPVREAVKAGLDEHGRLSAWTLHIVSPSITSRYDPTSKDPFDSVIEYVQNFPYA
                     VANFDLRYTRQEIGIDVGYWRSVSHAPNCFAIESSIDEIAALLSKDPLEFRRELLAGK
                     PRHLQVLKVIAERSGWGHASKGHFQGIAFMEGYTSHIAQVAEVSVEAGRLKLHKISCV
                     IDCGQTVNPRIVESQLESGIVYGLTAALWGNITLRGGRVQQRNFNDQRVLRINELPVL
                     DVHVLTSDAKPGGVGESAVPPVAPSICNAIFAATGKRLRTLPLAAHDLV"
BASE COUNT          377 a          715 c          723 g          402 t
ORIGIN      
        1 actaccgatt cgaggccgag tggcagctgg cgtgtgaacg aatgaccgct gcgggattcg
       61 ccgcagtgag ctcattcaac ccgtactgga acctgcgggg acggagcttc caggaccccg
      121 acggctatcg cgtggtactg cagcgggagg cgtgggctcc cgggcccgag ggtggactcg
      181 tctaaattag agggctcaga cgagatcgtg cgcagcgagc gggagcgtac gcagccgttt
      241 cccggtggcc gcgaagatgg cgttgcagat cgaaggtgcc accggaggta ccgcgctctc
      301 cccaacccca ccgggcttgg catcgcttgt cagcacgtgc acatcgagga ccggcagctc
      361 gttgatcctc agcacgcgct ggtcgttgaa gttgcgctgc tgaacccgac cgccgcgcag
      421 cgtgatgtta ccccaaagtg cggccgtcag gccgtacacg atgccacttt cgagctgcga
      481 ttcgacgatg cgtggattca cggtttgtcc gcagtcgatg acgcagctga tcttgtgcag
      541 cttgagcctc ccggcctcga cggacacctc ggccacctgc gcgatgtgtg aggtgtagcc
      601 ttccatgaag gcaatgccct gaaagtgtcc cttggacgca tggccccagc cggagcgctc
      661 ggcgatgacc ttgagcacct gcaggtggcg cggcttgccc gcgaggagct cccggcgaaa
      721 ttccagcggg tccttggaca ggagagcggc gatttcgtcg atcgagctct cgatggcgaa
      781 gcaattcggt gcgtggctca ccgaacgcca gtagccgacg tcgatgccga tctcttgacg
      841 cgtgtatctc aggtcgaagt tcgccacggc ataggggaag ttctgcacat actcgatcac
      901 cgaatcgaaa ggatccttgc tggtcggatc gtagcgcgag gtgatggaag gggagacgat
      961 gtgcagcgtc caggcgctga gcctgccgtg ctcatccagg ccggctttca ccgcctctcg
     1021 caccggcggc cggtacaagt cgtgagacat gtcatcttcg cgggtccaga tcagtttcac
     1081 gggcgcgccg accgccttcg aggccgcgac cgcagcgggc acgaaatcca catccaggcg
     1141 acgaccgaag ccgccgccga gcagagtggt gtgcacgttg acctgattcg ccttgaggcc
     1201 cgctgcctct gcggcagctg cctgcgccag ctgctgtgcc tgcgtcccga cgtagaggtc
     1261 gcaactattc gcccggacat ccgccgtgca attcatcggc tccataggcg catgcgccag
     1321 gagcggtagc tcgtagaccg cagcgaacgt cttggccgct cgcttcaggg cttccgccgc
     1381 gtggccgccc ttgagggcgg ccgccacatt gtcgctcgcg agggctgaga ccccgggttt
     1441 cgccgcggcc gcgttcagtt gcgcccagat cgccgcgttg tcgagctgtg cattcggtcc
     1501 ggctgcccac acgatgcgca aggcatcgcg cgctttcttg gcctgccaga aatgctcggc
     1561 cacaacgatg accgcgctcg cggtcggcac gatgcggcgc acacccggca tggcaagtgc
     1621 ggcggcagaa tccaccgaag ccaccgtccc gcccagttcc ggacagaggg cgatggcggc
     1681 gtgcagcatg ccggggagct tcacgtcgag gccaaactga gcgctgccgt ccaccttgcc
     1741 cggtgtatcg agacgtggca aagacttgcc gataaggcga aattcgcccg ggctcttgag
     1801 tggcacctct ttggggacgg ggactttggc cgccgcgtcg gccagctcgc cgtaactggc
     1861 ggatctgccc gaggcgctcg tgatacggcc gttgctcgca cgacagctcg agggatcgac
     1921 ccgccagcgc cgcgccgccg ccgtgatcag catcgaacgc gcctgggcgc ccgctttgcg
     1981 caacttctcc caggcatccg caatgctgtt gctggtgccg gtgatctgac cgctgttgag
     2041 cgcgttcaca tacgcttcgc ccaccggtgc ggcgacgacc ttcacttgag aggggtcgat
     2101 ctcgagctct tcagccagca gcgtgggcaa ggcggtgtaa accccttgcc ccatttctga
     2161 gcgatcgacg aggatggtaa tggagtcgtc gctgccgatt ctgacccagg cattcaa
//
LOCUS       DATDMP010000238         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_814411, whole genome shotgun sequence.
ACCESSION   DATDMP010000238 DATDMP010000000
VERSION     DATDMP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814411"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VI195_04030"
     CDS             complement(<1..775)
                     /locus_tag="VI195_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEY6823581.1"
                     /translation="MVTAADLRVAQGYVRCGRCSSVFNALARLSDERQAAGGADQPAA
                     LSAQEPEEPAQPAATDLSPLDQALTRVDDDDATETSGATEEPISEAALEFNPETTDVG
                     AVFVEPPPNPQWTAATGSFKAMRAASQAESGDPQVDVEIDAAFLADMLREAAPQSDPS
                     PQPEQPPPPAPDVPPAASAASPVGPATPARTVKPAAPPALDPSAEEHAPPDPPSGVGP
                     APAAEESAREAARHVSVRTRPPAEASVRRRATMTREPAPR"
     gene            complement(837..1733)
                     /gene="prmA"
                     /locus_tag="VI195_04035"
     CDS             complement(837..1733)
                     /gene="prmA"
                     /locus_tag="VI195_04035"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY6823582.1"
                     /translation="MSSIGFIELAFDLGARPAQAAEEAAFDCGAIAVTFVDARDDAVL
                     EPAPGEMPLWPATRVRCLFERAAADPPELAALIGALAARLAIEVPQIEVQWVADRVWE
                     REWLKDFHALRFGRRLWICPQHERVCAPDAVVIMLDPGLAFGTGTHASTALCLTWLDV
                     HPPTDAQVIDYGCGSGVLGIAAARLGAAEVHAFDIDPQALIATRDNARANELQARLEV
                     HADAATLPRANDLLLANILAAPLCALAPCFASLVRAGGQVVLAGLMEHESAEVTAAYA
                     ACFDVIRFAERAGWVCLVGRRR"
     gene            complement(1767..3110)
                     /gene="accC"
                     /locus_tag="VI195_04040"
     CDS             complement(1767..3110)
                     /gene="accC"
                     /locus_tag="VI195_04040"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY6823583.1"
                     /translation="MIEKIVIANRGEIALRILRACRELGIKTVAVHSTADYSLTHVLL
                     ADESVCIGPPPSSQSYLNMPAIISAAEVTDSVAIHPGYGFLSENADFAERVENSGFVF
                     VGPRAESIRTMGDKVSAIRVMKGHGVPCVPGSDGPLGDEADENLRIARDIGYPVIIKA
                     SGGGGGRGMRVVHAEASLLNAIGITRAEARAAFGNDQVYMEKFLERPRHIEFQVLADG
                     LGNVIHLGERDCSMQRRHQKVIEEAPAPGISIRQRRLMGERCVEACRAVGYRSAGTME
                     FLYQDGEFYFIEMNTRIQVEHPVTELVTGIDLVKAQLLIASGERLPYTQSDVQIRGHA
                     IECRINAEDPKTFMPSPGPIRLWHAPGGPGVRVDSHVYSGYNVPPYYDSLIGKIIAQG
                     DTRDTAIARMKNALAEMVIEGIKTNVPLHQEIFNHAAFQKGGTDIHYLERRLGLR"
     gene            complement(3115..3588)
                     /gene="accB"
                     /locus_tag="VI195_04045"
     CDS             complement(3115..3588)
                     /gene="accB"
                     /locus_tag="VI195_04045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY6823584.1"
                     /translation="MDIRKIKKLIELLEESGIAEIEIKEGEEAVRISRMPVGASVQPP
                     QVTHLGAVPAGSTALAPAAGAEAATLESAAAKPKASEHVITAPMVGTFYASPSPGAKP
                     FVEIGDEIKVGQVLCIIEAMKMMNQIEADRAGRVASVMARNGDPVEFGQPLFVVE"
     gene            complement(3637..4083)
                     /gene="aroQ"
                     /locus_tag="VI195_04050"
     CDS             complement(3637..4083)
                     /gene="aroQ"
                     /locus_tag="VI195_04050"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY6823585.1"
                     /translation="MARVLLLNGPNLNLLGTREPSVYGTDTLEAIEQRAAAIARETGH
                     ELVAFQSNAEHELIERLHRARSEGVAFLIVNPGAFTHTSIALRDALLATGLPFIEVHL
                     SNPHARETFRRQSYFSDIAVGVISGFGAFGYEAAVRAAALRLGSGP"
     gene            complement(4208..>4629)
                     /locus_tag="VI195_04055"
     CDS             complement(4208..>4629)
                     /locus_tag="VI195_04055"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6823586.1"
                     /translation="NFWATWCEPCRREIPLLKRLRREYAGNGLEIVGIGIDQLDPVRK
                     FASEQAIDYPLLLGERDGLEAARAFGMETVVPFSVFSDREGRVVTLKVGELHRDEAEL
                     ILDRIREVDAGRLSLIAAQAQISKGVGHLREVRAPQD"
BASE COUNT          759 a         1543 c         1534 g          793 t
ORIGIN      
        1 ttcgcggggc aggctcgcgc gtcattgtcg cgcgccgacg cacgctcgcc tctgcgggag
       61 gccgtgtgcg gactgagaca tggcgcgcag cctctcgcgc actctcttca gcggcgggtg
      121 caggccctac gccgctgggc ggatcgggtg gcgcgtgctc ctccgctgaa ggatcgagcg
      181 ctggcggggc cgcgggtttg acggtgcgcg ccggcgtggc gggcccgacg ggcgaggcgg
      241 ccgaggccgc cggcggcacg tccggcgccg gcggcggggg ctgctcgggc tgcggtgacg
      301 ggtcggactg cggcgcggct tcccgcagca tgtcggccag gaaggccgcg tcgatctcaa
      361 cgtccacctg cggatcgccc gactcggcct gactggccgc ccgcatggcc ttgaaagagc
      421 cggtggcggc ggtccactgc gggttgggtg gaggctcgac gaacaccgct cccacatctg
      481 tggtctcggg gttgaattcc agcgctgcct cggatatcgg ctcctccgtt gcaccgcttg
      541 tctcggtggc gtcatcgtcg tccacgcggg tcaatgcttg atccagcggg gagagatccg
      601 tagcggctgg ctgcgctggc tcctccggtt cctgagccga gagggccgca ggctgatccg
      661 cgccgccagc ggcttgccgc tcatcgctca ggcgcgcgag cgcgttgaac acactcgagc
      721 agcgtccgca acgcacgtag ccctgcgcga cccgcaggtc ggccgcggtc accaccaggg
      781 tcagcgcaca cttcgggcag actgtgaaca tcgtaggcgt gtttcagcgc ctgtggtcag
      841 cgacgccgac cgacgaggca aacccagccg gcgcgctcgg caaaccggat gacatcaaag
      901 catgcagcat aagcggctgt cacctccgcc gactcatgct ccatcagacc ggccagcacg
      961 acttgtccac ccgcgcgcac cagtgaggcg aagcagggcg ccaatgcgca cagcggtgcg
     1021 gccaggatgt tggcgagcag caggtcgttc gctcggggca gcgtcgctgc gtcggcgtgc
     1081 acctcgagcc gcgcctgcag ctcattcgcg cgggcgttgt cgcgcgtggc gatgagcgcc
     1141 tgcgggtcga tgtcgaaggc gtgcacctcg gcagcgccga gtcgcgcagc tgcgatgccg
     1201 agcacgccgg agccgcagcc atagtcgatg acctgcgcat cggtcggtgg atggacatcg
     1261 agccaggtga gacacagcgc agtgctggca tgcgtacccg tgccgaaggc gagtccggga
     1321 tcgagcatga tcacgaccgc atccggcgcg caaacgcgct cgtgctgcgg acagatccac
     1381 agacgccggc cgaaacgcag cgcatggaag tctttcagcc attcgcgctc ccacacccgg
     1441 tcggcgaccc actgcacctc gatctgcggg acttcgatgg cgagccgggc ggcgagcgca
     1501 ccgatgagcg ccgcgagctc cggcggatcg gccgctgcgc gctcgaacag acatcgcacc
     1561 ctggtcgcgg gccacagtgg catttccccg ggggcaggct cgagcaccgc gtcatcgcgt
     1621 gcatcgacga aggtgacggc aatggcaccg caatcgaacg ctgcttcttc cgccgcctgc
     1681 gccggccggg cgccgagatc gaaggccagc tcgataaagc cgatgctcga cacaaacact
     1741 ccgcgagcgg gtgcagctcg cgccgctcag cgcagaccca ggcggcgctc caggtaatga
     1801 atgtcggtgc cgcccttctg gaatgccgcg tggttgaaga tctcctggtg cagcggcacg
     1861 ttggtcttga tgccctcgat caccatctcc gccagggcat tcttcatgcg cgcaatcgcg
     1921 gtatcgcgcg tgtcgccctg ggcgatgatc ttgccgatga gcgagtcata gtacggcggc
     1981 acgttgtagc cggagtacac gtggctgtcg acccgcacgc caggtccacc cggtgcatgc
     2041 cacagccgaa tcggaccagg cgagggcatg aaggtctttg gatcctcggc gttgatgcgg
     2101 cattcgatgg catgaccgcg aatctgcacg tcgctctgcg tgtatggcag ccgctcgccg
     2161 ctcgcgatca gcagctgcgc cttgaccaga tcgatgccag tgacgagctc ggtaaccgga
     2221 tgctcgacct ggatacgggt gttcatctcg atgaagtaga actctccgtc ctggtagaga
     2281 aattccatgg tgccggcgct gcggtagccg accgcacggc acgcctcgac gcagcgctcg
     2341 cccatcaagc gccgctgacg aatgctgatg ccaggtgcgg gcgcctcctc gatgactttc
     2401 tggtgccgcc gctgcatcga gcagtcacgc tccccgagat gtatgacgtt gcccagccca
     2461 tccgcgagaa cctggaactc gatgtgccgc ggccgctcga ggaacttctc catgtagacc
     2521 tgatcgttgc cgaaggcggc acgtgcttcc gcgcgcgtga tgccgatggc attgaggagc
     2581 gaagcctcgg cgtgcaccac gcgcatgccg cgaccgccgc ccccgcccga ggccttgatg
     2641 atcaccgggt agccgatatc gcgcgcgatg cgcagattct cgtccgcttc atcgccgagc
     2701 ggtccgtccg agccgggcac gcagggcacg ccgtgtccct tcatcacgcg aatggcggat
     2761 accttgtcgc ccatcgtgcg gatcgactcc gcccgcggcc cgacgaacac gaagccgctg
     2821 ttttcgacgc gctcggcgaa gtcggcgttc tcggagagga acccgtagcc cgggtggata
     2881 gcgacggaat cggtgacctc ggccgccgag atgatcgccg gcatgttgag ataactctgc
     2941 gaggagggcg gcggcccgat gcataccgac tcatccgcga gcaatacgtg cgtgaggctg
     3001 tagtcggcgg tggagtggac cgccaccgtc ttgatgccga gctcgcggca ggcgcgcagg
     3061 atccgcagcg cgatctcgcc acggttggcg atgacgatct tctcgatcat gcggctattc
     3121 gacgacgaac agcggctggc cgaattccac cggatcgccg ttgcgagcca tgaccgaggc
     3181 gacccggccc gctctgtcgg cctcgatctg gttcatcatt ttcatggcct cgatgatgca
     3241 caacacctgg ccgaccttga tctcgtcgcc gatctcgacg aagggttttg cgcccgggga
     3301 gggcgaggcg tagaaggtgc ccaccatcgg ggcggtgatg acgtgctcgc tcgccttggg
     3361 tttcgccgca gcgctctcca gggtggcagc ttctgcgccg gctgccggcg ccagcgccgt
     3421 ggagcccgcc ggcaccgcgc cgaggtgcgt cacctgcggc ggctgcacgc tcgccccgac
     3481 aggcatgcgg ctgatgcgta ccgcctcttc cccctccttg atctcaatct ccgctatgcc
     3541 ggactcctcc agcagctcga tcagtttttt gatttttcga atgtccatgc cggaactgcc
     3601 cccaagagaa tcgccagacc gggcaccgcc tcggtgtcag ggcccgctgc cgagccgcaa
     3661 cgctgcggct cgtaccgccg cctcatagcc gaatgcaccg aagccgctga tcaccccgac
     3721 agcgatgtcc gagaaatacg attgccggcg gaaggtctcg cgggcatgcg ggttggacag
     3781 gtgtacctcg atgaatggca gacctgtcgc cagcaacgca tcgcgcagcg cgatgctcgt
     3841 gtgggtgaac gcgcccgggt tgacaatgag gaacgccacc ccttcggagc gcgccctgtg
     3901 gagacgctcg atgagctcgt gctccgcgtt gctctgaaag gcgaccagct catgacccgt
     3961 ttcgcgcgcg atggcagctg cacgctgctc gatcgcctcg agggtgtcgg ttccgtacac
     4021 ggacggctcg cgggtcccga gcagattgag gttcgggccg ttcaacaaca gcacacgggc
     4081 catgacccta ggacttcttt gtttgattcg tgcaagcgac cggccgcgaa tgtaacctaa
     4141 tccgcagcca ttcgggtaca agttgttgcg atttgcgaag aacgtgcagg atctgggcca
     4201 ggtcgcccta gtcctggggg gcgcgcacct cccgcaggtg acccacccct tttgagatct
     4261 gagcctgggc ggcgatgaga gagagccttc cggcatccac ctcgcggatg cggtcgagga
     4321 tgagctccgc ctcgtcccga tggagctcac cgaccttgag ggttaccacc cgcccttccc
     4381 ggtccgagaa cacgctgaag ggaactacgg tctccatacc gaaggctctg gcggcctcga
     4441 gcccgtcgcg ctccccgagc aggagggggt agtcgatcgc ctgttccgag gcgaatttgc
     4501 gcacgggatc gagctgatcg atgccgatgc cgactatttc gagaccattc cctgcatatt
     4561 cgcgtctaag tctcttcaga agcgggattt cgcggcggca cggctcgcac caggtcgccc
     4621 agaaattga
//
LOCUS       DATDMP010000239         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2035925, whole genome shotgun sequence.
ACCESSION   DATDMP010000239 DATDMP010000000
VERSION     DATDMP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035925"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VI195_04060"
     CDS             complement(<1..501)
                     /locus_tag="VI195_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY6823587.1"
                     /translation="MHEQVRDIVRVPLSLLTRLRLILFGHEDLAGIIFWAALIGICGA
                     LCSVAFREGTRLLELLFTGHSQSLVHAAAALSWWHRAIVPVIGGVLAGLVLHVLARKL
                     TLARAVDYMEAVLVGDGRIGFRATLVNAFSSLLTIASGGSIGREGAMVQLAAMMGSRL
                     GLLAHAP"
     gene            631..1017
                     /locus_tag="VI195_04065"
     CDS             631..1017
                     /locus_tag="VI195_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEY6823588.1"
                     /translation="MISGYSDHAANERTFLAWIRTGIAVIAFGFVIEKFNLFLLTLAE
                     AGLSNEVHRSQLASLSGRIGRYDGLVLIAGGVALVVVATVRFVRTTRLLDDAQAHAAS
                     SVRTELVLCAALVLVVASYSLYLAFS"
     gene            complement(1246..1446)
                     /locus_tag="VI195_04070"
     CDS             complement(1246..1446)
                     /locus_tag="VI195_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823589.1"
                     /translation="MLPLLECIWALQAFFMSLFDMLGLLACCCVMQSFICCSCDLPLV
                     LLLLLELLDGEDDDCDELLLLG"
     gene            complement(1528..2808)
                     /locus_tag="VI195_04075"
     CDS             complement(1528..2808)
                     /locus_tag="VI195_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6823590.1"
                     /translation="MRSLRALAALLLLLTPLPAALAASTVVTAARMIDVLSGRVVERP
                     QILITGERITSVGRQGDPAPADAKRIDLGSRTVLPGLIDMHVHLTSDPHYSGYRTLQF
                     TDAFWMTVGVANARRTLESGFTTVRNVGSSNYDDVGLKQGIEQGYIEGPRIVPATYAI
                     GPTGGHCDATEFPPSITSPSTQIANSPDEYRTLVRKLHKYGAEIIKVCMTGGVMSKGD
                     SVGAQQLSFEEIQAIVQEAHRLGLRVAVHAHGTAGINDALRAGVDTIEHASLADSESF
                     TLAKKHGAWFDMDIYNDDYILAEGEKNGVFPESLAKERAIGRKQRENFRAAHAAGVKM
                     LFGTDAGNYPHGTNARQFAKMVEWGMTPMEAIQAATRSAAEALDRTADVGALAPGRYA
                     DLIAVDGDPLSDIRVLESVAVVMKGGQIVKGGGQ"
BASE COUNT          501 a          971 c          963 g          503 t
ORIGIN      
        1 gggggcgtgc gccagcagtc cgaggcgcga gcccatcatc gcggcgagct gcaccatcgc
       61 cccctcgcgg ccgatcgagc cgccggaggc gatggtgagg agcgaggaga acgcattgac
      121 cagcgtggcg cgaaagccga tgcgtccatc accgaccaac accgcctcca tgtagtcgac
      181 ggcgcgcgcc agagtgagct tgcgtgcgag cacatgcagg acgagccccg cgagtacgcc
      241 gccgatcacc ggcacgatgg cgcggtgcca ccagctgaga gcggcggcag cgtggaccag
      301 actctgcgag tggccggtga agagtagctc gagcagccgc gtgccttcgc gaaacgccac
      361 gctgcagagc gcgccgcaga taccgatcaa cgccgcccag aaaatgatgc cggcgagatc
      421 ctcgtgaccg aacaggatga gccgcaatcg cgtcagtagc gaaagcggca cccgtacgat
      481 atcgcgaacc tgctcgtgca ttgcctcgat ggtagtgcac gcatagcgcc gggtcgcaac
      541 actcgcagcc gcggcagcgt acgcgtcagc gagctcggac ttgggcgcct cggttatgat
      601 gcgcgcgacc gacagccggg agtgcggcac atgatcagcg gttacagcga ccacgcggcg
      661 aacgagcgta cctttctcgc ctggatccgc acgggcatcg ccgtgatcgc ctttggcttc
      721 gtcatcgaga aattcaatct gttcctgctg acgctcgccg aggctggttt gagcaacgaa
      781 gtacaccgtt cgcagctcgc gagcctttcg ggccgtatcg gccgctacga cggactggtg
      841 ctcatagcgg gtggcgtcgc gttggtcgtg gtggccacgg tgcgcttcgt gcgcaccacg
      901 cgcctgctcg atgatgcgca ggcgcatgcg gcgagcagcg tgcgcaccga gctcgtgctg
      961 tgcgccgcgc tggtgctcgt ggtggcgagc tacagcctct acctggcctt cagttaggcc
     1021 tgcagcgcga gcgccctggg gttgccgccc gcgtaagctg ccgcctacgc cgacacgcga
     1081 cctgcgcagg attctttcca ggggctcacc ggtacattct tgctcgccct gcaaccgatc
     1141 ggaggagccc atgaaacgct tagccgcatc cttcgccgtg ctcgcgctcg ccgccgctgg
     1201 gatcgtcgca gcacagacga cttcgccttc gtcaccgagc tcggcctatc cgagcaacag
     1261 cagctcatcg cagtcgtcat cctcgccgtc gagcagctct agcagcagca gcagcaccag
     1321 cggcaagtca cacgagcagc agatgaagga ctgcatgacg cagcagcagg ccaacaatcc
     1381 caacatgtcg aagagcgaca tgaagaaggc ctgcaaggcc cagatgcact ccagcagcgg
     1441 cagcaccccg cacgagtagt cgcagcgatt gcggcagcga ggcccgggcg gcagcggccg
     1501 tccgggcctt tgtcgtctgg gtaagcgcta ttgcccgccg cccttgacga tctggccacc
     1561 tttcatgacg accgccaccg actcgagcac gcgaatgtcg ctgagcggat cgccgtcgac
     1621 ggcgatcaga tcggcataac ggccgggcgc gagcgcgccg acatccgcgg tgcggtcgag
     1681 cgcttcggcg gcgctgcgcg tggccgcctg gatggcctcc atcggggtca tgccccactc
     1741 gaccatcttg gcgaactgcc gggcgttggt gccgtgcgga tagttgccgg cgtcggtgcc
     1801 gaacagcatc ttgactccgg cggcgtgcgc ggcacggaag ttctcgcgct gcttgcggcc
     1861 gatcgcccgc tccttcgcca ggctttccgg gaacaccccg tttttctccc cctcggcgag
     1921 aatgtagtca tcgttgtaga tgtccatgtc gaaccaggca ccgtgcttct tcgccagcgt
     1981 gaagctttcg gaatcggcga ggctcgcatg ctcgatggtg tcgaccccgg cacgcagagc
     2041 atcgttgatc ccggcggtgc cgtgcgcgtg taccgccacg cgcagcccga gccgatgagc
     2101 ttcttgcacg atcgcctgga tctcttcgaa tgacagctgc tgcgcaccga cgctgtcgcc
     2161 tttggacatc acgccaccgg tcatgcacac cttgatgatc tcggcgccgt acttgtgcaa
     2221 tttacgcacc agtgtgcggt actcatccgg cgagttggca atctgcgtgc tcggggaggt
     2281 gatcgaggga ggaaactctg tggcatcgca gtggccaccg gtcggaccga tggcgtaggt
     2341 ggccggcacg atgcgcggac cctcgatgta gccttgttcg atcccctgct tgagtccgac
     2401 gtcatcgtag ttgctcgagc cgacgttgcg cacggtggtg aagccgcttt cgagcgtgcg
     2461 gcgcgcgttg gcgacgccca ccgtcatcca gaaggcgtcg gtgaactgca gtgtgcggta
     2521 gccgctgtag tgcggatcgg aggtgaggtg cacatgcatg tcgatcagac ccggcaacac
     2581 ggtgcgtgaa cccagatcga ttcttttcgc atccgcgggc gccggatcgc cctgtcgccc
     2641 gacgctggtg atgcgctcgc cggtgatgag gatctgcggc cgctcgacca cccgtccgct
     2701 caacacatcg atcatgcgcg ccgcggtaac gaccgtgctc gccgcgagcg ccgcgggcag
     2761 cggggtgagc agcagcagca gcgcggcgag cgcacgcagg gaccgcatca tttcattgcc
     2821 tttcagcaag agctcggtgg gcgtgtcagg gcgatgaccg gcgcggattc ttccggaatg
     2881 cgcaccgctc agtcaaccgc cgcggcggct gcggtaagat cgccggcttc tccgccta
//
LOCUS       DATDMP010000240         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_592400, whole genome shotgun sequence.
ACCESSION   DATDMP010000240 DATDMP010000000
VERSION     DATDMP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592400"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VI195_04080"
     CDS             <1..739
                     /locus_tag="VI195_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823591.1"
                     /translation="ERWGYGVNVLTYLVILTALLRMRLKTHAHAPTERGLLRQLAAGA
                     RFAFGFLPIRTALLLLAATSVTVQSYAALMPWFAREAFHGDSRTLGILVSGAGLGAVS
                     GMLYLASRRNIRGLLRLIAWTSGTAGTGLVVFSFSSSLWVALPALYFVGMGLMLSAAS
                     TNTVIQSIVPDELRGRVASLYVMAFIGMSPIGAFLSGLLAERIGPPATLACCGVLALG
                     AALAYASRLGAIRREIRPLYEKLGIPS"
     gene            complement(736..1362)
                     /locus_tag="VI195_04085"
     CDS             complement(736..1362)
                     /locus_tag="VI195_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823592.1"
                     /translation="MTSRREWLTGAAALLLVRRGLAEGSIETGVRTFFGEVRINGKAA
                     RRGMSVGAGDTISTADGAQVVFVVNRDAFLVRKESEASVLKTGLRIVTGAVLSVFAPG
                     GRRQIETGTAVIGIRGTAVYVEAQADRSYVCTCYGEAVLEPRGDPGSRETVRTTHHEQ
                     PRYIMAKGAPQMMMRAPVVNHTDAELDMLEALVGRKPPFAGKGYKPYY"
     gene            complement(1346..1852)
                     /locus_tag="VI195_04090"
     CDS             complement(1346..1852)
                     /locus_tag="VI195_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823593.1"
                     /translation="MARWIRLGAVERAVFDAACSALAAAQARSAPPIVLWSEEAAYYP
                     FALLAPLKYAPGRRDRWLSWGLASAVATYRQFRVPAYLDDELRLHGRNIADSSAGQVG
                     ECAVVASSFLMRFPGNCVATPSFELEQAFRLRLEAQHGWQFEHSWPNASESRQDARLN
                     DRLNDLAP"
     gene            complement(1917..2414)
                     /locus_tag="VI195_04095"
     CDS             complement(1917..2414)
                     /locus_tag="VI195_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY6823594.1"
                     /translation="MRRSGAALGAMLFISVLRVHAEEATFNARLLTPAAALKAAQAAL
                     ASCRASGFQVTVAVVDRMGVVQVLLRDRFAGPHTVDMASAKAYTAVSFRTSTRELADA
                     TQPGKPQSGVRARPGVAAVAGGLMIEAGGSLLGAVGVSGAPGGAEDEKCAAAGLEAIR
                     EDLEL"
     gene            complement(2411..2638)
                     /locus_tag="VI195_04100"
     CDS             complement(2411..2638)
                     /locus_tag="VI195_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY6823595.1"
                     /translation="MDFNKELDARGLNCPLPILRTKKALADMQSGQVLKVLATDPGSV
                     KDFQSFSKQTGHELVSHSEQDREFTFFMRKK"
     gene            2688..>2870
                     /locus_tag="VI195_04105"
     CDS             2688..>2870
                     /locus_tag="VI195_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823596.1"
                     /translation="MNTAGHWLLLIAQLPTEDPASRMRILRTLESLGAAVIREGAYLL
                     PRSPSNRQALEALADYI"
BASE COUNT          434 a         1021 c          930 g          485 t
ORIGIN      
        1 cgagcgctgg ggctacggcg tgaacgtgct cacctacctc gtcatcctga ccgcgctttt
       61 gcgcatgcgc ctcaagaccc acgcccatgc gccgacggag cgcggactgc tgcgccagct
      121 cgcggcgggc gcgcgcttcg cgttcgggtt cctgccgatc cggacggcgc tgctgctcct
      181 cgcggcgacc agcgtgacgg tgcaatcgta cgcggcgctc atgccatggt ttgcgcgtga
      241 agccttccac ggcgattcgc gcacgcttgg catcctcgtc agcggcgcgg gcctcggcgc
      301 ggtatcgggc atgctgtacc tcgcctcgcg ccgcaatatc cgggggcttt tgcgcctcat
      361 cgcatggacc tcgggcaccg cgggcaccgg gctcgtagtg ttttcgtttt ccagctcgct
      421 atgggtggcg ctgcccgccc tctatttcgt aggcatgggc ctcatgctct ccgccgcctc
      481 cacgaacacg gtgatccaga gcatcgttcc ggacgagctg cgcggacgcg tggcttccct
      541 ctacgtgatg gcattcatcg gcatgtcgcc gatcggggcg ttcctctctg ggctgcttgc
      601 cgagcggatc ggcccgccgg ccacgctggc ctgctgcggc gtgctcgccc tcggggcggc
      661 gcttgcgtac gcgagccggc tcggcgccat ccggcgcgag atccggccgc tctacgagaa
      721 gctcggcatt ccgtcctagt agtacggctt gtagcccttg ccggcgaacg ggggcttgcg
      781 tcccacgagg gcctcgagca tgtcgagctc cgcatccgtg tggttcacga ccggcgcgcg
      841 catcatcatc tgcggtgcgc ccttcgccat gatgtagcgc ggctgctcgt gatgcgtggt
      901 gcgcaccgtc tcccgggacc ccgggtcgcc gcgcggctcg aggacggcct cgccgtaaca
      961 ggtacagaca tagcttcggt cggcctgcgc ctccacgtag accgccgtgc cgcggatgcc
     1021 gatcacggca gtgccggttt ctatctggcg ccggccgccc ggcgcgaaaa ccgagagcac
     1081 tgcgcccgtg acgattcgca ggccggtctt caggaccgaa gcctcgcttt ccttgcgcac
     1141 caggaaagcg tcccggttca cgacaaatac cacctgggcg ccgtcagccg tggaaatcgt
     1201 gtcgcccgca cccacgctca tgccgcgccg cgcggccttg ccgttgatgc gcacctcgcc
     1261 gaagaacgtg cgaacgccgg tttcgatgct gccctcggcc aagccgcgac gcacgaggag
     1321 cagcgccgcc gcgccggtca gccactcacg gcgcgaggtc attcagccga tcgttcagcc
     1381 gcgcgtcctg gcgcgattcc gaggcgttcg gccacgagtg ctcgaactgc cagccatgct
     1441 gggcttccag gcgcagacgg aaggcctgct cgagctcgaa ggaaggcgtc gcgacgcaat
     1501 tcccgggaaa gcgcatgagg aagctcgacg ccacgacggc gcattcgccg acctgcccgg
     1561 cgctgctgtc ggcaatgttt cggccatgca gccgcagctc gtcgtccagg taagccggga
     1621 cgcggaattg gcggtaggtg gccacggcgg aggcgagccc ccaggagagc caccggtcgc
     1681 gccggcccgg cgcgtacttg agcggcgcga gcagcgcgaa ggggtagtaa gccgcttcct
     1741 ccgaccagag cacgatcggc ggcgcgcttc tcgcctgcgc cgccgcaagt gcgctgcagg
     1801 ccgcatcgaa aaccgcgcgc tccaccgcgc ccagcctgat ccagcgagcc atcatgcgat
     1861 ccattattcc atgaaggcgg tgagcatcgc tctgcaacgc gatggaaaaa gcgctctcat
     1921 agctccaagt cctcgcggat tgcttccagc cccgcggccg cgcatttctc atcctcggcg
     1981 ccgcccggcg ccccggatac gcccaccgcg ccgaggagcg agccacccgc ctcgatcatc
     2041 aggcctccgg ccaccgccgc cacgccaggc cttgcgcgca ccccgctttg cggcttgccc
     2101 ggctgcgtag catcggcaag ctcgcgcgtg cttgtacgga agctcaccgc ggtgtatgcc
     2161 ttcgcgctcg ccatgtcgac ggtatgcgga ccggcgaagc gatcgcgcag caatacctgg
     2221 acgacgccca ttcgatccac gacggcgacc gtgacctgaa acccgctggc ccggcagctc
     2281 gcgagcgcgg cttgcgcggc cttgagcgcc gcggccggag tgagcagacg cgcgttgaac
     2341 gtggcctcct cggcgtgcac gcgcaggacg gaaatgaaaa gcatggcgcc gagcgcggca
     2401 cccgagcgcc tcacttcttc ctcatgaaga aggtgaactc gcggtcctgt tcgctatgcg
     2461 acacgagctc atgcccggtc tgcttcgaaa agctctgaaa atccttgacc gagccgggat
     2521 ccgtggcgag caccttgagc acctgcccgg actgcatgtc ggcgagggcc ttcttggtgc
     2581 gcaggatagg cagcgggcag ttgaggccgc gggcgtcgag ttccttgttg aaatccatgc
     2641 acggctccgt tgcgaggcgc ccagtatatg atgcaataaa cgccgccatg aacaccgcgg
     2701 gccattggct gttgttgatc gcgcagctcc ccacggagga tcccgcttcg cgcatgcgca
     2761 tccttcgcac gttggagtcg ctcggggccg ccgtgatccg agaaggcgcc tatctgctgc
     2821 cgcgcagccc ttccaaccgc caggccctgg aggcgctggc cgactacatc
//
LOCUS       DATDMP010000241         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_703886, whole genome shotgun sequence.
ACCESSION   DATDMP010000241 DATDMP010000000
VERSION     DATDMP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703886"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VI195_04110"
     CDS             complement(<1..397)
                     /locus_tag="VI195_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823597.1"
                     /translation="MNPVLFYDPACQRPYDTRTLHTQALGGTEATLVRVADALGAWVM
                     QHNRTQDWQRYLRPQRLAGITHVVINRDSRALPFVQQHYPGARVYLWLHDRLHPRARR
                     ARWLAATAPLLRANAVTAVCVSDWQRGGVE"
     gene            448..1215
                     /locus_tag="VI195_04115"
     CDS             448..1215
                     /locus_tag="VI195_04115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6823598.1"
                     /translation="MADDSGLSACIITFNEADRIEACVRSVDFCDEILIVDAHSSDRT
                     RELAASLGARVIERDWPGYRSQKQFAVDSARHDWVLCLDADERVSLQLRAEILALRRA
                     GLASAAGWSIPRITDYFGRFLRHGNAYPDRLVRLFDRRRGGWTGYEIHENTRIEGRVR
                     RLHGHLEHYSYRSLSDHQLRMQRYADLMAQALYERGRRCGLLPVLLNPQWRFLRGYLL
                     RLGFLDGWRGLVFALVESGYVRRKYLGLYMRSRGLPG"
     gene            complement(1183..1782)
                     /locus_tag="VI195_04120"
     CDS             complement(1183..1782)
                     /locus_tag="VI195_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY6823599.1"
                     /translation="MSPTCELPLFPLSTVLFPGGPLRLRIFEPRYLDMVRSCLKASRA
                     FGVVLILEGEEAGEVVSVAGSGTSARVVDFDTLPDGLLAIDCLGEQGFRVLKRWQQPD
                     GLNIGEVEYLPEDVPCALPAELVHLGELLRELLPQLGERYAHVVTRYEDAGWVANRWA
                     EVLPLTAAEKQQLLELADPLARLAQVAAWSTRQPPTAHV"
     gene            complement(1779..3164)
                     /gene="mpl"
                     /locus_tag="VI195_04125"
     CDS             complement(1779..3164)
                     /gene="mpl"
                     /locus_tag="VI195_04125"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY6823600.1"
                     /translation="MHVHILGICGTFMGGIAAIARAAGFTVTGSDRNVYPPMSTQLAA
                     LGIALTEGFDAAQLAPAPDVVVVGNVMTRGQPVVEALLESTLPYFSGPEWLAREVLKD
                     RWVLAVAGTHGKTTTASLLAWILEHAGHDPGFLIGGVPANFGVSARLGSGPFFVIEAD
                     EYDTAFFDKRAKFVHYRPRTVALNNLEYDHADIYPDIGTIQRQFHHLVRIVPGSGRIV
                     WNAADALLRETLAMGCWTPLEGFARSARSDALWSARPLPGAADYSRFEVLEGGRPLGT
                     VEWSLIGAHNMENALAAIAAARHAGVEVPRALAALADFKGIARRLQLRGEVNGIRVYD
                     DFAHHPTAIATTLDGLRQRVGKARIIAVLEPRSHTMRMGVHEHTLAPALAGADEVWLY
                     TPADLGWDTGTVLRALAGRGHGSADIAALARELARAARPGDQVLIMSNGGFGGLHDKL
                     LTELQNAMPRP"
     gene            complement(3173..>3406)
                     /locus_tag="VI195_04130"
     CDS             complement(3173..>3406)
                     /locus_tag="VI195_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823601.1"
                     /translation="PLVDSCAWLVRRIGEDSPWQMPFICGLLLLFAWVIAANSHIVKA
                     ALGWSATSSVALVILQTLAGWLLLSALFPAVKA"
BASE COUNT          558 a         1164 c         1168 g          516 t
ORIGIN      
        1 cctcgacacc gccgcgctgc cagtccgata cgcagacggc ggtaaccgcg ttggcgcgca
       61 gcagcggcgc ggtggctgcc agccagcgcg cgcgccgtgc gcgcggatgc aaccggtcgt
      121 gcagccacag gtacacgcgc gcaccgggat agtgctgttg cacgaacggc agggcgcgcg
      181 aatcgcggtt gatcacgaca tgcgtgatgc ccgccaggcg ctgcggccgc agataacgct
      241 gccagtcctg cgtacggtta tgctgcatca cccacgcgcc gagcgcatcc gcgacgcgca
      301 ccagcgtcgc ctcggtgccc cccaacgcct gcgtgtgcag cgtacgggtg tcgtaaggcc
      361 gttggcaggc agggtcgtag aacaggaccg ggttcatctg agtgtgtatt gtacggctgt
      421 gtcgaccaac cgacggtgag ggcgttcgtg gcggatgaca gcggcttatc ggcgtgcatc
      481 atcacgttca acgaagccga ccggatcgag gcctgcgtgc gctccgtcga tttctgcgac
      541 gagatcctga tcgtcgacgc ccactcgagc gatcgcacgc gcgagctcgc cgcctcgctc
      601 ggcgcgcgcg tcatcgagcg cgactggccg ggctatcgct cgcagaagca gttcgcagtg
      661 gacagcgccc gccatgactg ggtgctgtgc ctcgacgccg acgagcgtgt cagcctgcag
      721 ctgcgtgcgg agatcctggc gctgcggcgc gccgggctgg catcggccgc gggctggtcg
      781 ataccgcgca tcaccgacta tttcggccgc tttctgcgcc acggcaacgc ctatcccgac
      841 cgtctggtgc ggctgttcga ccggcgtcgc ggcggctgga cgggttacga gatccacgag
      901 aacactcgca tcgagggtcg cgtccgccgc ctgcacggac acctcgagca ctattcctac
      961 cgcagcctca gcgatcacca gctgcgcatg caacgctacg ccgacctcat ggcgcaggca
     1021 ctctacgaac gcggccggcg ctgcggcttg ctgccggtgc tgctcaatcc gcagtggcgc
     1081 ttcctgcgcg gctatctact gcggctggga ttcctcgatg gctggcgcgg gctggtgttc
     1141 gcgctcgtcg agtccggcta cgtgcggcgc aagtatctcg ggctatacat gcgcagtcgg
     1201 gggctgccgg gttgaccatg cggccacttg cgcaagccgc gcgagcgggt cggcgagctc
     1261 gagcagctgc tgtttctcgg cggcggtgag cggcagtacc tcggcccagc ggttggcgac
     1321 ccaaccggca tcctcataac gcgtcacgac gtgcgcgtag cgctcgccga gctgcggcag
     1381 cagctcgcgc aacagctccc cgagatgcac gagctccgcg ggcagtgcgc acggcacatc
     1441 ctcgggaaga tactcgactt cgccgatgtt gaggccgtcc ggctgctgcc agcgcttgag
     1501 cacgcggaag ccctgctcac cgaggcagtc gatggcgagc aatccatcgg gcagcgtgtc
     1561 gaaatccacg acccgcgcgc tggtgccgct gcccgccacg ctcaccacct caccggcttc
     1621 ctcaccctcc agaatgagca ctacgccgaa ggcacgcgag gccttgaggc agctgcgcac
     1681 catgtcgagg taacggggct cgaagatgcg caggcgcagc ggtccgcccg gaaacagcac
     1741 ggtgctgagc ggaaacagcg gcagctcgca tgtgggactc acggccgtgg catcgcattc
     1801 tgcagctcgg tgagcagctt gtcatgcagt ccgccgaaac cgccgttgct catgatgagc
     1861 acctgatcgc ccgggcgcgc ggcgcgggcg agctcgcgcg ccagtgccgc gatatcggcg
     1921 ctgccatggc cgcggccggc aagggcgcgc agcaccgtgc cagtgtccca gccgagatcc
     1981 gccggggtat acagccatac ctcgtccgcc ccggcgagcg cgggggcgag cgtgtgctcg
     2041 tgcacgccca tgcgcatggt gtgcgagcgc ggctcgagta cggcgatgat gcgcgccttg
     2101 ccgacccgct ggcgcaggcc gtcgagggta gtggcgatcg cggtcggatg gtgcgcgaag
     2161 tcgtcgtaca cgcggatgcc gttgacctcg ccgcgcagct gcaggcggcg cgcaatgccc
     2221 ttgaagtccg cgagtgcggc gagcgcacgc ggcacctcga ctccggcgtg acgtgccgcg
     2281 gcgatcgcgg cgagcgcatt ctccatgttg tgcgcgccga tcagagacca ctcgaccgtg
     2341 ccgagcggcc ggccgccttc gagcacctcg aagcgcgagt agtcggcagc cccaggcagc
     2401 ggtcgcgcgc tccatagcgc atcgctgcgt gcgctacgcg cgaagccttc gagcggggtc
     2461 cagcagccca tggcgagcgt ttcgcgcagc agcgcatcgg ccgcgttcca gacgatgcgt
     2521 ccgctgccgg gaacgatacg caccagatgg tgaaactgcc gctggatggt gccgatgtcc
     2581 ggatagatgt cggcgtgatc gtactcgaga ttgttgagcg ccacggtacg cgggcggtag
     2641 tgcacgaact tggcgcgctt gtcgaagaac gccgtgtcgt actcgtccgc ctcgatgacg
     2701 aagaacggac cactaccgag gcgcgcgctc acgccgaagt tggcgggaac tcccccgatg
     2761 agaaagcccg gatcgtgccc ggcatgctcg agaatccagg cgagcagcga agcggtcgtg
     2821 gtcttgccgt gcgtgccggc caccgcaagt acccagcggt ccttgagcac ctcgcgggcc
     2881 agccactcgg gaccggagaa ataaggcagc gtgctctcga gcagcgcttc caccaccggc
     2941 tggccgcgcg tcatgacgtt gcccaccacc accacatccg gggccggcgc gagctgagcg
     3001 gcatcgaagc cctcggtgag cgctatgccg agcgccgcga gctgcgtgct catcggtgga
     3061 tagacgttgc ggtccgagcc ggtcacggtg aaaccggcgg cgcgcgcgat ggccgcgatg
     3121 ccgcccatga aggtgccgca aatcccgagg atgtgaacgt gcagcggcgc cttcatgcct
     3181 tcaccgccgg aaacagcgcc gacagcagca gccagccagc gagagtctgc aggatgacga
     3241 gcgccacgct cgaggtcgcc gaccacccga gcgccgcctt cacgatgtgg ctgttggcgg
     3301 cgatgaccca cgcgaacagc agcagcagcc cgcagatgaa aggcatctgc cagggcgagt
     3361 cttcgccgat gcgccgcacc agccaggcgc agctgtccac cagcgg
//
LOCUS       DATDMP010000242         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2036839, whole genome shotgun sequence.
ACCESSION   DATDMP010000242 DATDMP010000000
VERSION     DATDMP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036839"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VI195_04135"
     CDS             complement(<1..1002)
                     /locus_tag="VI195_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6823602.1"
                     /translation="MSLSELFIRRPVATSLLTIGFALAGLVAFRLLAVAPLPQVDFPT
                     VSVQVQLPGASPETMAATVATPLERALGRIAGVTEITSTSTLGSSRITLQFALDRDID
                     GAARDVQAAINAARGQLPSGLPNNPTYRKVNPADAPIMILALTSDTLDRGRMYDAAST
                     ILAQKLSQVAGVGQVIVSGSALPAVRVELNPTALNKHGVSLDDVRAAINSANANRPKG
                     VVEEGERQWQIYANDQARKADEYRSLIVAYRNGAPLRLSDVAQVDDSVQDLRNQGLSN
                     GKPAVLVIIFKQPGANIIETADRVRDVLPQLEASIPSSINVIVAMERTSTIRASLREV
                     "
     gene            complement(999..>2256)
                     /locus_tag="VI195_04140"
     CDS             complement(999..>2256)
                     /locus_tag="VI195_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6823603.1"
                     /translation="KRDGAEEVMHRLQQRLSDTEGIKLYMQPVQDLTIEDRVSRTQYQ
                     FTLETADPAELSKWTHLLVNRISQLTQVADVASDLQDEGLQAYLEIDRDTAGRLGITP
                     ASIDSALYNAFGQRLISTIFTQSNQYRVVLEVKPEFRKGPNALSEIYVATASGTQVPL
                     STVTRLVEKPATLAINHIGQFPAATISFNLARGYALGDAVKAIEGVQSELGVPVSIRT
                     SFQGAASAFRSSLSNTLLLILAAIVTMYIVLGVLYESYIHPITILSTLPSAGVGALLA
                     LIVARSDLGVIGIIGIILLIGIVKKNAIMMIDFALDAERKEGKSPRDAIYQACLLRFR
                     PILMTTMAALLSALPLMLGTGVGSELRHPLGITMVGGLIVSQMLTLFTTPVIYLWFDR
                     LARRFDTRVPVAEGDGIGEAAEERRP"
BASE COUNT          355 a          702 c          814 g          385 t
ORIGIN      
        1 gacctcgcgc aaggacgcgc ggatggtgct ggtacgctcc atcgcgacga tgacgttgat
       61 ggagctcggg atcgaggcct cgagttgcgg caggacgtcg cgcacgcggt ccgcggtctc
      121 gatgatgttg gcgcccggct gcttgaagat gatgacgagc accgccggct tgccgttcga
      181 caggccctgg ttgcgcaggt cctgcaccga gtcgtcgact tgggcgacgt cggaaaggcg
      241 cagcggagct ccgttgcggt aggcgacgat cagcgagcga tactcgtccg ccttccgcgc
      301 ctggtcattg gcgtagattt gccactggcg ctcgccttcc tccaccacgc ccttgggccg
      361 gttggcgttg gcgctgttga tcgccgcgcg cacgtcatcg aggctgaccc cgtgcttgtt
      421 gagcgcggtg gggttcagct cgacgcgcac cgcgggcagc gcgctgccgc tgacgatgac
      481 ctgcccgacg ccggccacct gcgagagttt ctgcgcgagg atcgtggagg cggcgtcgta
      541 catgcgcccc cggtcgagcg tgtcggaggt gagcgcgaga atcatgatcg gcgcatccgc
      601 cgggttcacc ttgcgatagg ttggattgtt gggcagaccc gatggcagct ggccgcgcgc
      661 ggcgttgatc gccgcctgta cgtcgcgcgc cgcgccgtcg atatcgcgat cgagtgcgaa
      721 ctggagcgtg atgcgagacg agccgagcgt cgaagtcgag gtgatctccg ttactcccgc
      781 gatgcggccg agcgcacgct cgagcggcgt cgccaccgtc gcggccatgg tctcggggct
      841 cgcgcccggc aactgcacct gcaccgagac ggtggggaaa tccacctgcg gcaggggggc
      901 caccgccagc agccggaacg cgaccaggcc cgcgagtgcg aagccgatcg tgaggagcga
      961 ggtggcgacc gggcgccgga tgaagagctc ggagaggctc acggtcgccg ctcctccgcg
     1021 gcttctccga tcccgtcacc ctccgctacg ggcacccggg tgtcgaagcg gcgcgcgagg
     1081 cggtcgaacc agaggtagat caccggcgtg gtgaacagcg tgagcatctg gctcacgatc
     1141 aggccgccaa ccatggtgat gccgagcgga tggcgcagct ccgagccgac gccggtgccc
     1201 agcatcagcg gcagcgcgga gagcagcgcc gccatcgtcg tcatcaggat cggccggaag
     1261 cgcagcaggc aggcctggta gatggcgtcg cgcggactct tgccttcctt gcgctcggcg
     1321 tcgagcgcga agtcgatcat catgatcgcg ttcttcttga cgatgccgat cagcaggatg
     1381 atgccgatga tgccgatgac gccgagatcg ctgcgcgcga cgatcagcgc gaggagcgct
     1441 ccgacgcccg ccgagggcag ggtggagagg atcgtgatgg ggtggatgta gctctcgtag
     1501 agcacgccca gcacgatgta catcgtgacg atggcggcga ggatcagcag cagcgtattg
     1561 gagagcgagg agcggaacgc gctcgccgcg ccctggaagc tggtgcggat gctgaccggc
     1621 acgcccagct cgctctgaac gccctcgatg gccttcaccg cgtcgccgag cgcgtagccg
     1681 cgggcgaggt tgaacgagat ggtcgcggcg ggaaactggc cgatgtggtt gatcgcgagc
     1741 gtggccggct tctcaaccag gcgggtgacg gtgctgaggg gcacctgcgt accgcttgcg
     1801 gtagcaacgt agatttcgga cagtgcattg ggacccttgc ggaactcggg tttcacttcc
     1861 agcaccaccc ggtactggtt ggactgggtg aagatggtcg agatcaggcg ctgcccgaac
     1921 gcgttataga gcgcgctgtc gatcgacgcc ggcgtgatgc cgaggcggcc ggcggtgtcg
     1981 cggtcgatct ccaggtaggc ctgcagtccc tcgtcctgaa gatcgctggc gacgtcggcg
     2041 acctgcgtga gctgggaaat ccggttcacc agcaggtgcg tccacttcga gagctccgcc
     2101 gggtccgccg tctcgagcgt gaactggtac tgggtgcggc tcacgcggtc ctcgatggtg
     2161 agatcctgca ccggttgcat gtagagcttg atcccttcag tgtccgagag ccgctgttgc
     2221 agccggtgca tcacttcctc ggccccgtcg cgcttc
//
LOCUS       DATDMP010000243         3239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_926540, whole genome shotgun sequence.
ACCESSION   DATDMP010000243 DATDMP010000000
VERSION     DATDMP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926540"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VI195_04145"
     CDS             complement(<1..1323)
                     /locus_tag="VI195_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823604.1"
                     /translation="MVAEVPAGTGAVAAAGTQAQAWPARSAGYYGLFVIILATALNFL
                     DAQIFNMMAPRIKADLHLTDEQLGFLLGPANIVFYVLVGIPLARLVDLYPRKYVLAGG
                     ITLIGGITALGGLAQNFVQLFMSRMLVGAGGSAHAPGSYSMLADYFPPARLPRAIGFL
                     QLGFISGNVLGFYLGGLLLTMVAAWPVTHWMGLTIHGWQWVLMMVGTPGLVIAALLLC
                     AQEPPRRGLAGEAQNVPFSAVLREIWTRRGVYLPLFIGLAFSATEFLGLQVWRPTFLA
                     RTYGWSPQRIGNWMSVVTLVSWLVGAFVGTVFTEWLAKRYKDANVRASTILFALIVPF
                     EVAAPLMPTGELSLVCVALGGVCGLASAVPQNAAIQRITPNAMRGRVTAIYLFMFIFF
                     GALGGQLIGSVTQRVFGADSALWKSILLTAATLVPLAAFTISRGIRPYG"
     gene            1389..2297
                     /locus_tag="VI195_04150"
     CDS             1389..2297
                     /locus_tag="VI195_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823605.1"
                     /translation="MQNLAGKTAFVTGGASGIGLGIAKALLGAGMNVVIADIREDHLA
                     QASEELGTPERVLALKLDVTDRAAYARAADAAEARFGKIHLLCNNAGVAVIGPTELAT
                     FADWDWVLGVNLGGVVNGIVVLLPRILKHGEGGHIVNTSSMSGLVPHGGATIYGTSKG
                     AVLSMMECMRPELESRGVICSAFCPGAVQSNIAEAGRTRPATLADSGYAEADKRRQQN
                     TSFFHLFRTKEEVGARVLQGILNDELYILTHSEFRQGVEERAQAMCAAVPELPENEEY
                     KRTFSFLFRNPVHAAESARQRKRGKT"
     gene            2294..3193
                     /locus_tag="VI195_04155"
     CDS             2294..3193
                     /locus_tag="VI195_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823606.1"
                     /translation="MKDFAGRTAFVTGGANGVGIGLVRALLAQGCNVALADIRRGSID
                     AALQALDNPRAMGVEVDVSSRADLARAADAVEAKFGVVTLLFNNAGVNLFQSIEESSY
                     DDWDWIMGVNLHGPINGVMTFVPRMIRAARGGYVVNTASMAGLLAAGTCGIYNTTKFA
                     VRGLSGSLRASLAAHGIGVSVLCPGLVKSYIYASDEIRPEALKAGAKPVNSEFVQRLA
                     QVHEFGMEPDVIAARVLEAMREDRFHIFSHPEFKDELRELFGEILDDFRDYPVDPGHA
                     QRIDFEKTRRESYRKQRRGLKGA"
BASE COUNT          595 a         1049 c         1103 g          492 t
ORIGIN      
        1 gccataaggc cggatgccgc gcgagatcgt gaatgccgcg agcggcacca gggtcgcggc
       61 cgtcaaaagg atgcttttcc agagcgcgct gtccgcgccg aagacacgtt gcgtcacgct
      121 gccgatcagc tgtccgccga gtgccccgaa gaaaatgaac atgaagagat agatcgcggt
      181 cacccggccg cgcattgcat tcggcgtgat gcgctggatg gccgcgttct gcggcaccgc
      241 cgaggcgagc ccacacaccc cgcccagcgc gacgcatacg agcgagagct ctcccgtcgg
      301 catgagcgga gcggcgactt cgaacgggac gatgagggcg aagagtatcg tggaggcgcg
      361 cacgttggcg tccttgtaac gcttggccag ccactcggtg aacaccgtgc cgacaaaggc
      421 gcccacgagc catgacacca gagtgacgac gctcatccag ttaccgatgc gctgtgggct
      481 ccagccgtag gtgcgggcga ggaaggtcgg gcgccagacc tggagaccaa gaaactcggt
      541 ggcgctgaaa gcgaggccga tgaaaagcgg caggtagacg ccgcgccgcg tccagatctc
      601 gcgcaagacg gcgctgaacg gtacgttctg cgcctccccc gccagacccc gccgcggcgg
      661 ctcctgggcg cagagcagaa gggccgcgat cacgaggccg ggtgttccga ccatcatgag
      721 cacccactgc cagccatgta tggtgagacc catccagtgc gtcaccggcc acgccgccac
      781 catggtgagc agcaggccac ccaggtagaa gccgagcacg ttgccactga tgaagccgag
      841 ctgcaggaag ccgatggcac gcggcagtcg cgccggcgga aaataatccg cgagcatcga
      901 gtacgagccc ggggcgtgcg ccgagccgcc ggcacccacc agcatgcggc tcatgaacaa
      961 ctgcacgaag ttctgcgcga gcccgccgag cgcggtgatg ccgccgatga gcgtgatgcc
     1021 gccggcgagc acgtacttgc gcgggtagag gtccaccagg cgcgcgagcg gaatgccgac
     1081 cagcacgtag aagacgatgt tggcgggtcc aaggagaaac ccaagctgct cgtcggtaag
     1141 gtgtaagtct gccttgatgc gcggggccat catgttgaag atctgcgcgt cgaggaaatt
     1201 gagcgccgtg gcgaggatga tcacgaacag gccgtaatac ccggcgctgc gcgcgggcca
     1261 cgcctgcgcc tgcgtaccgg cagccgccac ggcacctgtg cctgcgggca cttccgcgac
     1321 catcgtgctt cccccgcgcg ctgcgttttg ccagcgccag actgtatgtg ttagatacaa
     1381 tatgactcat gcagaacctg gcaggcaaga ccgcattcgt gaccggaggc gccagcggca
     1441 tcggcctcgg cattgccaag gccctgctcg gggccggcat gaatgtcgtc atcgcggata
     1501 tccgcgagga tcatctcgcg caggcgagtg aagagctcgg cacgcccgaa cgggtgctgg
     1561 cgctgaagct cgacgtgacc gatcgcgcgg cttatgcgcg cgccgcggac gcggccgaag
     1621 cgcgcttcgg caagatccac ctgctctgta acaacgcggg cgtggcggtg atcggaccga
     1681 ccgagctcgc gacctttgcc gattgggact gggtgctcgg agtgaacctg ggtggcgtgg
     1741 tgaatggcat cgtcgtgctg ctgccgcgca tcctcaagca cggcgagggc ggccacatcg
     1801 tcaacacctc ctcgatgtcc gggctcgtgc cccatggggg cgccacgatc tacggcacct
     1861 ccaagggcgc agtgctcagc atgatggagt gcatgcgccc cgagctcgag tcgcgcggtg
     1921 tcatctgctc ggccttctgc ccgggcgcgg tgcagtcgaa catcgccgag gccggcagga
     1981 cgcgtccggc cacactcgcg gattcgggct atgccgaggc cgacaagcgg cgccagcaga
     2041 acaccagctt cttccacctg ttccgcacca aggaggaggt tggcgcacgg gtactgcagg
     2101 gaatcctgaa cgacgagctc tacatcctga cccacagcga gttccgccag ggggtggagg
     2161 agcgcgcgca ggcgatgtgc gcggcggtgc ccgagcttcc ggagaatgag gagtacaagc
     2221 gcactttcag ctttctgttc cgcaacccgg ttcacgccgc tgagagcgcg cgccagcgca
     2281 agcgaggcaa gacatgaagg actttgccgg ccgcacggcc ttcgtcaccg gcggcgcgaa
     2341 cggcgtcggc atcggcctgg tgcgggcact gctcgcgcaa ggctgcaacg tcgccctcgc
     2401 cgacatccgc cgtggctcga tcgatgccgc actccaggcg ctcgacaacc cgcgcgccat
     2461 gggcgtcgag gtggatgtat cctcgcgcgc cgacctggcg cgtgccgccg atgccgtcga
     2521 ggcgaagttc ggcgtggtca cgctgctgtt caacaacgcc ggcgtcaacc tgttccagtc
     2581 gatcgaggag tcgagctatg acgactggga ctggatcatg ggcgtgaatc tgcacggccc
     2641 gatcaacggc gtgatgacct tcgtgccccg tatgatccgc gccgcgcggg gcggctacgt
     2701 ggtgaatacc gcctcgatgg cgggcttgct cgccgccggc acctgcggca tctacaacac
     2761 caccaagttt gcggtgcgcg ggctctcggg atcgctgcgc gccagcttag cggcgcacgg
     2821 tatcggcgtg tcggtgctgt gccccgggct ggtgaagtcc tatatctacg cgagcgatga
     2881 gatccggccg gaggcgctga aggccggtgc caagcccgtg aatagcgagt tcgtgcagcg
     2941 cctggcgcag gtgcacgagt tcggcatgga gccggacgtg atcgccgcgc gggtgctgga
     3001 agcgatgcgc gaggatcgct tccacatctt cagccatccg gagttcaagg acgagctgcg
     3061 cgagctgttc ggcgagattc tcgatgactt ccgcgactac cccgtcgacc cgggccacgc
     3121 gcagcgcatc gacttcgaga agacgcgccg cgagagctac cgcaagcagc gtcgcgggct
     3181 caaaggagcc tgagcgtgca cgtcgaggcg ctcgatcacg tcaacgtcat caccgccga
//
LOCUS       DATDMP010000244         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1704195, whole genome shotgun sequence.
ACCESSION   DATDMP010000244 DATDMP010000000
VERSION     DATDMP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704195"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VI195_04160"
     CDS             <1..681
                     /locus_tag="VI195_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY6823607.1"
                     /translation="RPDTALASMGIYVFAADFLYDQLRRDAADPHSQHDFGKNIIPHI
                     VQHGHAQAHRFSRSCVRNAGEKESYWRDVGTVDAYFDANLDLTNVVPDLDIYDQDWPI
                     WTYQVQQPPAKFVLDEEGRRGSAINSMVSGGCIISGAVVRESLLFSNVRVEERTNIYR
                     SVILPNAEIGRDCIIRGAIVDEGGDIPDGTTIGVDREADARRFHVTEKGVVLVTPDML
                     RALRSVSR"
     gene            678..>2029
                     /locus_tag="VI195_04165"
     CDS             678..>2029
                     /locus_tag="VI195_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEY6823608.1"
                     /translation="MTATPRLPVILLWHMHQPHYRDALTGRYALPWTYLHAIKDYTDM
                     AAHLEGNREARCVVNFTPLLIEQLEEIAARIAAHLESGSALPDPILALLGPESVPTDP
                     AARLELLRGCLRAQRRQMIERFGPYLELATIAETLGTPERVSYASDQFIHDLAVWYHL
                     AWVGETVRRADPLIAHLTERGRDFTLAERRALLELIGRLVGEIIPRYRQLAERGQCEL
                     SVTPYGHPIMPLLLDFHCARDAVPAMTLPARAAYPEGAARARWHIEEALRVFARAFGA
                     APAGCWPAEGAISAATLELLAASGGFRWAASSASVLRNSLALADADAAQDPQAYNRPY
                     RLGGTGMSCFFRDDALSDLIGFTYATWHGDDAAANLVNELLQLARDYEQGGNHAVLIA
                     LDGENAWEHYPFNGYYFLRALYAQLANHPRLELTTLSDCLSRGIQPAPLPRVCAGSWV
                     "
BASE COUNT          318 a          736 c          648 g          327 t
ORIGIN      
        1 cggcccgaca ccgcgctggc ctcgatgggc atctacgtct tcgccgccga cttcctctac
       61 gaccagctgc ggcgcgacgc cgccgacccg cactcgcagc acgacttcgg caagaacatc
      121 atcccccaca tcgtccagca cggccacgcc caggcgcacc gcttcagccg ctcctgcgtc
      181 aggaacgcgg gcgagaagga gagctactgg cgcgacgtcg gcacggtcga cgcctacttc
      241 gacgccaacc tcgacctgac gaacgtggtg ccggacctcg acatctacga ccaggactgg
      301 ccgatctgga cctatcaggt gcagcagccg ccggcgaagt tcgtgctcga cgaggagggc
      361 cgccgtggca gcgccatcaa ctccatggtg tccgggggct gtatcatctc gggcgcggtg
      421 gtgcgcgagt cactactgtt ctccaacgta cgcgtcgagg agcgcaccaa catctaccgc
      481 tcggtcatcc tgccgaacgc cgagatcggc cgcgattgca tcattcgcgg tgccatcgtc
      541 gatgagggcg gcgatatccc cgatggcacc acgatcggtg tcgaccgcga ggcggacgca
      601 cgtcgctttc atgtgaccga gaaaggcgtg gtgctcgtca cccccgacat gctgcgcgcc
      661 ctgcgcagcg tcagccgatg accgcaacgc cgcgcctgcc ggtgatcctg ctgtggcaca
      721 tgcaccagcc acactaccgc gacgccctca ccgggcgcta cgcactgccg tggacgtacc
      781 tgcacgccat caaggactac accgacatgg ccgcgcatct cgagggcaac cgcgaagcgc
      841 gctgcgtcgt gaacttcact ccactgctga tcgagcagct cgaggagatc gccgcgcgca
      901 tcgccgccca cctcgagagc ggcagcgcgc tgcccgatcc catcctggcg ctgctgggac
      961 cggagagcgt gccaaccgat ccggctgcga ggctcgagtt gctgcgcggc tgcctgcgtg
     1021 ctcagcgccg gcagatgatc gagcgctttg gcccctatct ggagctggcg accatcgccg
     1081 agacgctcgg cacacccgag cgcgtgagct atgcctcgga ccagttcatc cacgatctcg
     1141 ccgtctggta ccacctcgcc tgggtcggag agaccgtgcg ccgcgccgac ccgctcatcg
     1201 cgcatctcac cgagcgcgga cgcgacttca cgctggcgga gcggcgcgcg ctgctcgagc
     1261 tgatcggccg gctggtcggc gagatcattc cccgttaccg gcagctggcc gagcgtggtc
     1321 agtgcgaact gtcggtcacg ccctatgggc atccgatcat gccgttgctg ctcgactttc
     1381 actgcgcccg cgatgcggtg cccgcgatga cgctgccggc gcgtgcggcc taccccgagg
     1441 gcgccgcgcg tgccaggtgg cacatcgagg aggcgctgcg tgtcttcgcg cgcgcgttcg
     1501 gggcagctcc ggcgggctgc tggccggccg agggcgcgat cagcgcagca acgctcgagc
     1561 tgctcgctgc ttccggcggt ttccgttggg cggcgagcag tgccagcgtg ctacgcaaca
     1621 gcctcgcgct cgccgatgcc gatgctgcgc aggacccgca ggcctacaac cgcccgtacc
     1681 gcctcggcgg caccggcatg agctgcttct tccgtgacga tgcgctctcg gatctgatcg
     1741 gcttcaccta cgccacctgg catggcgatg acgccgcggc caacctcgtg aacgagctgc
     1801 tgcagctggc gcgcgactac gaacagggcg gcaatcacgc ggtgctcatc gcactcgatg
     1861 gggagaatgc ctgggagcac taccccttca acggttacta ttttctgcgc gcgctgtatg
     1921 cgcagctcgc caaccatccg cgcctcgagc tcacgacgct ctcggactgt ttgtcgcgcg
     1981 gcatccagcc ggcgccgctc ccccgagtgt gcgcgggcag ctgggtgca
//
LOCUS       DATDMP010000245         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_704592, whole genome shotgun sequence.
ACCESSION   DATDMP010000245 DATDMP010000000
VERSION     DATDMP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704592"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VI195_04170"
     CDS             <1..675
                     /locus_tag="VI195_04170"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HEY6823609.1"
                     /translation="LAAGLAEAAGTQQLTLAMAQEVAAGGHRRFDKGGDQFYDQISAL
                     HKSVRGTDPDGALYWLCRMLDGGCDPIYLARRVTRMAVEDVGLADPRALTLCIDAWQA
                     YERLGTPEGELAIATAVVYLACAPKSNAVYRAMGEAMADVQEFGTLDVPLRLRNAPTR
                     LMKDLGYGRGYRYAHDEPDAFAAGERYLPDELPDRRYYRPVPRGLEVKIGEALARLRS
                     HPPTKG"
     gene            681..1961
                     /gene="serS"
                     /locus_tag="VI195_04175"
     CDS             681..1961
                     /gene="serS"
                     /locus_tag="VI195_04175"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY6823610.1"
                     /translation="MIDPKLLRSDPEAVGRNLARRGFVLDVSALKALEEKRKPWAVEV
                     DRLRAERNAQARSVGQAKARGEDASALMARGEGLANELARAESQLESVQRELEQWQLG
                     LPNLLHASVPDGRDESANSELACWGEPRRFEFPARDHVELGERLGGLDFEAATRISGS
                     RFVVMRAQVARLHRALGQFMLDLHTREHGYTEVYVPYLVRPEALVGTGQLPKFAADLF
                     AVHGGPQAYFLIPTAEVPVTNLVRGEILSAEALPLKYVAHTPCFRSEAGAAGKDTRGM
                     IRNHQFDKVELVQIVRPADSYAALEELTAHAETVLQRLDIPYRRVALCAGDVGFGSAK
                     TYDLEAWLPSQQRYREISSCSNYEAFQARRMQARWRNPQSGKPEPVHTLNGSGLAVGR
                     TLVAVLENYQQADGSVSVPSALQPWLGGLTKLTA"
     gene            1996..2820
                     /locus_tag="VI195_04180"
     CDS             1996..2820
                     /locus_tag="VI195_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY6823611.1"
                     /translation="MKRPSRKDGSLRPRDPLFNSSVQKALAMLETFGGERRGLGLAEL
                     AAAAGMTTSSAQRCIHTLLRLGYLRRDTRLRRWVLTVRALSLTEAYLSGHTLLEHATA
                     HLITLNQASGESVSLSEPDGTDMVFIARFPSLQPFHIHMPVGRRLPMYCTASGRAYLS
                     ALPAASARRILARARLSAHTPQTLTDLQQICKRIEAARESGYAWSDQEFYRGDVTIAA
                     PVLGEDGQPLAAVNISAPTSRWTLAQLRTKLAALLLQSAQAASLGHTRRPERRLVK"
     gene            2831..>3273
                     /locus_tag="VI195_04185"
     CDS             2831..>3273
                     /locus_tag="VI195_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6823612.1"
                     /translation="MPELSRSLQPRHVTMISIGGIIGAGLFVGSSASIAATGPAIVLS
                     YLITGTLVLLVMRMLGEMAVALPAVRSFTEFARAGLGPWAGFVAGWLYWYFWIIVVPV
                     EAIAGATILHGWLGLPAWLLGLVLMAVMTGVNLLSARSYGEFEFW"
BASE COUNT          500 a         1107 c         1133 g          533 t
ORIGIN      
        1 ctcgctgccg ggcttgccga ggcggcgggt acacagcagc tgaccctcgc catggcgcag
       61 gaggtggccg ccggcggcca ccgccgcttc gacaagggcg gcgatcagtt ctacgaccag
      121 atctctgcgc tgcacaagtc cgtgcgcggc accgatcccg acggcgcgct gtattggctg
      181 tgccgcatgc tcgatggggg ttgcgacccc atctacctcg cacggcgggt gacgcgcatg
      241 gcggtggagg atgtcggcct cgccgacccg cgcgcgctga cgctttgcat cgatgcctgg
      301 caggcctatg agcgcctcgg cacgcccgaa ggcgagctcg ccattgcgac cgcagtcgtg
      361 tacctcgcgt gcgcgccgaa gagcaacgcg gtgtaccgcg ccatgggcga ggccatggcg
      421 gacgtgcagg agttcggcac gctcgatgtg ccgctgcggc tgcgcaatgc cccgacgcgc
      481 ctcatgaagg atctcggcta cggacgcggc tatcgctatg cgcacgatga gcccgatgcc
      541 tttgcagccg gggaacgtta tctgccggat gagctgccgg atcggcgata ctatcggccg
      601 gtcccgcgcg ggctggaagt gaagataggc gaggcgctcg cacgactgcg gtcgcatccg
      661 ccaacgaaag gttgatccgc gtgatcgatc cgaagctgct gcgctccgat ccggaggccg
      721 tggggcgcaa cctcgcgcgc cgcggattcg tgctcgatgt gagcgcgctc aaggcgctcg
      781 aggagaagcg caagccctgg gcggtcgaag tcgaccgcct gcgcgccgag cgcaacgcac
      841 aggcaaggtc ggtcggtcag gccaaggcgc gcggggagga cgcgagcgcg ctgatggccc
      901 ggggcgaggg gctcgccaac gagctggcgc gcgcggagtc gcagctcgag agcgtgcagc
      961 gcgagctcga gcagtggcag ctgggtcttc ccaatctgct gcacgcgtcc gtgccggatg
     1021 ggcgcgatga gagcgccaat tcggagcttg cctgctgggg cgagccgcgc cgcttcgagt
     1081 ttccggcgcg cgatcacgtc gagctcggcg agcggctcgg cgggctcgac ttcgaagccg
     1141 cgacgcgcat ctccggcagc cgcttcgtgg tcatgcgcgc gcaggtggcg cgcctgcatc
     1201 gggcgctcgg gcagttcatg ctcgatctgc acacgcgtga acacggctac acggaagtgt
     1261 acgtccccta tctggtgcgg ccggaggcgc tcgtcggcac cgggcagctg cccaagttcg
     1321 cggcggacct gttcgccgta catggcggtc cacaggctta tttcctgatc ccgaccgccg
     1381 aagtacccgt caccaatctg gtgcgcggag agatcctgag cgccgaggcg ctgccgctga
     1441 agtacgtggc gcacacaccc tgtttccgct ccgaagcggg cgcggccggc aaggacacgc
     1501 gcggcatgat ccgcaatcat cagttcgaca aggtggaact ggtgcagatc gtgcgcccgg
     1561 cggattccta tgcggcgctc gaggagctca ccgcgcacgc cgagaccgtg ctgcaacggc
     1621 tcgacatccc ttaccgccgc gtggcgctgt gcgccggcga tgtcggcttc ggcagcgcca
     1681 agacctacga cctggaggcg tggctgccct cgcagcagcg ctatcgcgag atttcctcct
     1741 gcagcaacta cgaggcattc caggcgcggc gcatgcaggc gcgctggcgt aacccgcaga
     1801 gcggcaagcc cgagccggtc cacacgctga atggctcggg actcgccgtc gggcgcacgc
     1861 tggtcgcggt actggagaac tatcaacagg cggatggctc ggtgagcgtg ccgagtgcgc
     1921 tgcagccgtg gctggggggg ctgacgaagc tcaccgcata gactatcgat tatcgatagt
     1981 catatactgc attcgatgaa gcgcccatcg cgtaaggatg gctcgctgcg cccgcgcgac
     2041 ccgctgttca acagctcggt gcagaaggcg ctcgccatgc tggagacctt cggcggcgaa
     2101 cgccgcgggc tcggtctcgc ggagctcgca gcggccgcgg gcatgaccac cagctccgcg
     2161 cagcgctgta tccatacgct gctgcgtctc ggttacctgc gccgcgacac gcggctgcgc
     2221 cgttgggtgc tgacggtgcg cgccctgtcg ctcaccgaag cctacctgtc cggccacacg
     2281 ctgctcgagc atgccaccgc tcatctcatc acgctcaacc aggcgagcgg tgaatcggtg
     2341 agtttgtcgg aacccgacgg taccgacatg gtgttcatcg cgcgcttccc gagcctgcaa
     2401 ccgttccata tccacatgcc ggtcgggcgg cgcctgccga tgtactgcac cgcctcggga
     2461 cgcgcctacc tctcggcgct gccggcggcg agcgcgcggc gcattctcgc gcgcgcgcgc
     2521 ttgagcgcgc acaccccgca gacccttacc gacctgcagc agatctgcaa gcgcatcgaa
     2581 gcagcgcgcg aatcgggcta cgcctggtcc gatcaggagt tctatcgcgg cgatgtcacc
     2641 atcgccgccc cggtgctcgg cgaagacggc caaccgctcg ccgcggtgaa tatctccgcg
     2701 cccacgagcc gctggacgct ggcgcagctg cgcaccaagc tcgcagcgct gctgctgcag
     2761 agcgcgcagg cggcctcgct gggtcacacg cgcaggcccg agcggcgcct ggtgaagtga
     2821 cgggggcgag atgccggaac tcagccgctc gttgcaaccg cgccacgtca ccatgatctc
     2881 gatcggcggc atcatcgggg ccgggctgtt cgtcggcagc agcgcctcga tcgccgcgac
     2941 gggacccgcc atcgtgctga gctatctgat caccggcaca ctcgtgctgc tggtcatgcg
     3001 catgctggga gagatggcgg tggcgctgcc ggcggtgcgc tcgttcaccg agttcgcgcg
     3061 agcgggtctc ggcccctggg cgggtttcgt tgccggctgg ctgtactggt acttctggat
     3121 catagtcgtg ccagtggagg cgatcgccgg cgccaccatc ctgcacggct ggctgggctt
     3181 gcccgcctgg ctcctcgggc tcgtgctgat ggccgtgatg accggcgtca atctgctctc
     3241 ggcccgctcg tacggagaat tcgaattctg gtt
//
LOCUS       DATDMP010000246         4151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1037741, whole genome shotgun sequence.
ACCESSION   DATDMP010000246 DATDMP010000000
VERSION     DATDMP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037741"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2030
                     /locus_tag="VI195_04190"
     CDS             <1..2030
                     /locus_tag="VI195_04190"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001951.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY6823613.1"
                     /translation="IAIGCALIALAYGVVSVQWILAKPAGNETMQKIAAAIQEGAQAY
                     LNRQYTAISIVGAVLFVVLGFALGWLTAVGFLIGALLSGATGYIGMNISVRANVRTAQ
                     AATDGLNAALGVAFRGGAITGMLVVGLGLIGVAGYYTLLRQFTSIGAEGALHALVGLA
                     FGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCA
                     GMAADLFETYAVTIVATMLLGGLLFGGRGPDAAIAAVIYPLALGAASIVASIIGTFFV
                     STREGGKIMNALYKGVIVAGLVSAVAFHFITHRIMPGDSAALYGCTLIGLGLTAAMIM
                     ITEYYTATEYNPVRRIAAASQTGHATNIIAGLGVSMKATALPVIAVCLAIWGAYTLSG
                     LYGIAIAATAMLSMTGMIVALDAYGPITDNAGGIAEMAGLPREVRAITDPLDAVGNTT
                     KAVTKGYAIGSAGLAALVLFADYTHNLEAAGKTVNFALADPAVIIGLFIGGLVPYLFG
                     AMAMEAVGRAAGSVVNEVRRQFREIKGIMAGTDKPDYSRAVDLLTKAAIGEMIVPSLL
                     PILVPIVLVVVVNALMGRGAGVRALGGLLIGTIVTGLFVAISMTTGGGAWDNAKKYIE
                     DGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLLVPLL"
     gene            2027..2437
                     /gene="msrB"
                     /locus_tag="VI195_04195"
     CDS             2027..2437
                     /gene="msrB"
                     /locus_tag="VI195_04195"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY6823614.1"
                     /translation="MSSAEAFPVEKSPAEWRRELTPDAYRVLREHGTERAGSSPLNDE
                     HRAGTFHCGGCGAPLFAADTKFESGSGWPSFFRPIPGAVATREDRSLFMRRTEVHCAR
                     CGGHLGHVFPDGPEPTGERYCMNGVALRFEPDKL"
     gene            complement(2462..>4151)
                     /locus_tag="VI195_04200"
     CDS             complement(2462..>4151)
                     /locus_tag="VI195_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6823615.1"
                     /translation="RVAGEGAGAAQQRTLDALAEAIIATDDKGQITYMNRAAEHLTGS
                     DALQASGKSLEEIVSLVDETDRRLLSDPVHQVLVSGAPVTLSRRTLLLARSNGSERSI
                     ELSAAPIRNEAREVIGAALLLHDVTELRGLARQMSYQATHDALTGLVNRREFERRLEE
                     AIESGHRGDGQHVLCYLDLDRFKLVNDTSGHLAGDSMLREVAKLLRDAVRDSDTVARL
                     GGDEFGTLLIGCPLEKARQIADDLCRSVSDYRFVWKDRIFNIGVSVGLVEISRESGTL
                     EELLAAADTACYVAKKQGSGRVAVYSARDEALARHSGEIQWLHRLQIALKENRFHLYH
                     QVIVPAHGEDGGPAMEVLVRLQDEGGNELQPADFMRAAERYRLMGMVDRWVVQTTLAA
                     LGRGAIPLQPNRSVAINVSAQTLGDLQFLEFVVECLDSTGVTPAQVCFEIAESAVVAN
                     LDHARRFIGVLHGMGCQFALDNFGSGVGSFSNLKNLPLDYLKIDGSFTRNLARDNVNQ
                     AMVTAMIKMARTLNFKIIAEQVEDTAAIDAARKIGVDYLQGFAIGRPLPLSLAA"
BASE COUNT          613 a         1474 c         1394 g          670 t
ORIGIN      
        1 ggatcgccat cgggtgcgcc ctcatcgcgc tcgcctacgg cgtcgtctcg gtgcagtgga
       61 tcctggccaa gccggccggc aacgaaacca tgcagaagat cgccgccgcc atccaggaag
      121 gcgcgcaggc gtacctcaac cggcagtaca ccgccatctc gatcgtcggt gcggtcctgt
      181 tcgtcgtgct gggcttcgcg ctcggctggc tgaccgcggt cggcttcctg atcggcgcgc
      241 tgttgtcggg cgccaccggc tacatcggca tgaacatctc cgtgcgcgcc aacgtgcgca
      301 ccgcacaggc cgcgaccgac ggcctgaacg ccgcgctcgg cgtagccttc cgcggcggcg
      361 ccatcaccgg catgctggtc gtgggcttag gcctgatcgg ggtggccggc tactacacgc
      421 tgctgcggca gttcacctcg atcggcgccg agggcgcgtt gcacgccctg gtgggactcg
      481 ccttcggcgg ctcgctcatc tcgatcttcg cgcgcctcgg tggcggcatc ttcaccaagg
      541 gcgccgacgt cggcgcggac ctggtcggca aggtcgaagc tggcattccg gaagacgacc
      601 cgcgcaaccc ggcggtgatc gccgacaacg tcggcgacaa cgtgggcgac tgcgccggca
      661 tggcggcgga cctgttcgag acctatgcgg tgaccatcgt cgcgaccatg ctgctcggcg
      721 ggctgctgtt cggcggccgc gggcccgacg ccgcgatcgc cgccgtcatc tatccgctgg
      781 cgctcggcgc cgcctccatc gtcgcctcca tcatcggcac cttcttcgtc agcacgcgcg
      841 agggtggcaa gatcatgaac gccctctaca agggcgtgat cgtcgccggg ctggtatccg
      901 cagtggcgtt tcacttcatc acgcatcgca tcatgccggg cgactcggcc gcgctctacg
      961 gctgtacgct catcggcctg ggcctcaccg ccgccatgat catgatcacg gagtactaca
     1021 ccgccaccga gtacaacccg gtgcgccgca tcgccgccgc ctcccagacc ggccacgcca
     1081 ccaacatcat cgccgggctc ggggtgtcga tgaaggcgac cgcgctgccg gtgatcgcgg
     1141 tgtgcctcgc catctggggc gcgtatacgc tgagcggact gtacggcatc gcgatcgcag
     1201 cgaccgctat gctgtcgatg accggcatga tcgtggcgct cgatgcctac ggaccgatca
     1261 ccgacaacgc cggcggcatc gctgagatgg cgggcctgcc ccgcgaggtg cgtgccatta
     1321 ccgacccgct ggacgcggtc ggcaacacca ccaaggcggt caccaagggc tacgcgatcg
     1381 gctcggcggg cctcgccgcg ctggtgctgt tcgccgacta cacccacaac ctcgaggccg
     1441 ccggcaagac cgtcaacttc gcgctggcgg atcccgccgt catcatcggg ctgttcatcg
     1501 gcgggctggt accgtacctg ttcggcgcca tggccatgga ggccgtcggc cgcgccgccg
     1561 gatcggtcgt gaacgaggtg cgccgccagt tccgcgagat caagggcatc atggccggca
     1621 ccgacaagcc cgattattcg cgcgcggtcg acctgctcac caaggccgcc atcggcgaga
     1681 tgatcgtgcc gtcgctgctg ccgatactgg tgccgatcgt gctggtggtg gtggtcaacg
     1741 cgctgatggg ccgcggggcc ggggtgcgtg cgctcggcgg tctgctgatc ggtaccatcg
     1801 tcaccgggct gttcgtcgcc atctcgatga ccaccggtgg cggcgcctgg gacaatgcga
     1861 agaagtacat cgaggacggc caccacggcg gcaagggctc ggatgcgcac aaggcggcgg
     1921 tcaccggcga caccgtcggc gatccctaca aggacaccgc cggtcccgcc atcaacccgc
     1981 tgatcaagat catcaacatc gtggcgctgc tgctggtacc gctgttgtga gctccgcgga
     2041 ggcgtttccg gtcgagaaat cgcccgcgga gtggcggcgc gagttgacgc cggacgccta
     2101 ccgggtgctg cgcgagcacg gcaccgagcg cgccggcagc agtccgctca acgacgagca
     2161 tcgcgccggc actttccact gcggcggctg cggggcgccg ctgtttgccg ccgacaccaa
     2221 gttcgagagc ggcagcggat ggccgagctt cttccggccg atccccgggg cggtggcgac
     2281 ccgcgaggac cgctcgctgt tcatgcgccg taccgaagtg cactgcgcgc gctgcggcgg
     2341 gcacctcggc cacgtgttcc ccgacggccc cgagccgacc ggcgagcgct actgcatgaa
     2401 cggcgtcgcc ctcaggtttg aacccgacaa gctctgacgg cgcagggcgc caaagccaca
     2461 actaggccgc cagcgacagc ggcagcggcc gcccgatcgc gaacccctgc agatagtcca
     2521 cgccgatctt gcgcgccgca tcgatcgcgg ccgtgtcctc gacctgctcg gcgatgatct
     2581 tgaagttgag ggtgcgcgcc atcttgatca tcgcggtaac catcgcctga ttgacgttgt
     2641 cgcgggccag gttgcgcgtg aacgagccgt cgatcttcag gtagtcgagc ggcaggttct
     2701 tcagattgga gaacgagccg acgccggagc cgaaattatc gagcgcgaac tggcatccca
     2761 tgccgtgcag tacgccgatg aaacggcgcg cgtgatcgag gttggcgacc acggcgctct
     2821 cggcgatctc gaagcacacc tgcgcgggcg tgacaccggt gctgtccagg cactcgacca
     2881 cgaactccag gaactgcagg tcgccgagcg tctgggccga gacgttgatc gccacgctgc
     2941 ggttgggctg cagggggatc gcgccgcgcc cgagcgcggc gagcgtcgtc tgcacgaccc
     3001 agcggtcgac catgcccatg aggcggtagc gctcggcggc gcgcatgaag tccgcgggct
     3061 gcagctcgtt gccgccctcg tcctgcagcc gcaccagcac ctccatcgcc ggtccgccgt
     3121 cctcgccgtg cgccggcacg atcacctggt gatagaggtg gaagcggttc tccttgagcg
     3181 cgatctgcag gcggtgcagc cactggatct cgccgctgtg gcgcgccagc gcctcgtcgc
     3241 gcgccgaata caccgccacc cggccgctgc cctgcttctt ggcgacgtag caggcggtat
     3301 cggcggccgc cagcagctct tccagcgtgc cgctctcgcg cgagatctcc accagcccca
     3361 ccgaaacgcc gatgttgaat atccggtcct tccacacgaa gcggtagtcg ctcaccgagc
     3421 ggcacaggtc gtcggcgatc tgccgcgcct tctcgagcgg acagccgatg agcagcgtgc
     3481 cgaactcgtc gccgccgagc cgcgccaccg tgtcgctgtc gcgcaccgcg tcgcgcagca
     3541 gcttcgcgac ctcgcgcagc atgctgtcgc cggccaggtg accgctggtg tcgttgacca
     3601 gcttgaagcg gtccaggtcg aggtaacaca gcacgtgctg gccgtcgccg cggtggccgc
     3661 tctcgatggc ctcctcgagg cgccgctcga actcgcggcg gttcaccagc ccggttagcg
     3721 catcgtgggt cgcctggtag gacatctgcc gcgccagccc gcgcagctcg gttacgtcgt
     3781 gcagcagcag cgccgcgccg atcacttcgc gcgcctcgtt gcggatcggg gccgccgaca
     3841 gctcgatcga gcgctcgctg ccgttgctgc gcgcgagcag cagggtgcgc cggctgagcg
     3901 tcaccggcgc gccgctcact agcacctggt gcaccgggtc gctgagcagc cggcggtcgg
     3961 tctcgtcgac cagactcacg atctcctcca gcgacttgcc ggaggcctgc agcgcgtcgc
     4021 tgccggtgag atgctcggcg gcgcggttca tgtaggtgat ctggcccttg tcgtcggtgg
     4081 cgataatcgc ttcggccagc gcatcgagcg tgcgctgctg agcggccccg gcgccctcgc
     4141 cggccacgcg c
//
LOCUS       DATDMP010000247         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1260881, whole genome shotgun sequence.
ACCESSION   DATDMP010000247 DATDMP010000000
VERSION     DATDMP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260881"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /gene="secD"
                     /locus_tag="VI195_04205"
     CDS             complement(<1..1766)
                     /gene="secD"
                     /locus_tag="VI195_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY6823616.1"
                     /translation="MLEYPRWKYLLVAAVLLLALVFALPNIFGEDPALQVERKDHTAV
                     NADAQRALEAFLTQHGVHYGKSYIDSGRLMVRFTSVPDQLAARDAVNAQYADTYRTAL
                     SFAPRTPAALRALGLRPMPLGLDLRGGLYLLYQVDVNAAVTQALDGYAQDARRALVAA
                     HIPVKEVSTLAAGGAATDAVRVVLPPEADANAARGALGTALQGLSVSTQSLPSGSAIT
                     ATMTSAQIRERRDYALEQNMTTLRNRVNGLGVSEPIVQRQGVDRINVQLPGVQNSAEV
                     KDILGRVATLEFRLEDMQNNAYEAQQTGRVPLGSKLYTHTRFGRPVLLKREVIATGDQ
                     LTNATSGQSQEGPAVNIRLDARAGDAMLRTTRTNLNRRMAVVLIEKHRETSEVNGKKV
                     SREVTDEQVINDATIRGVFSNNFQITGLQAGEARELALLLKSGSLAAPLYPIEERAIG
                     PSLGRDNIDKGVTALIVGMGGVFVFMWIYYKTFGLVADLVLLANVVLLTALLSMMRAS
                     LSLPGIAGIILTVGMAVDANVLIYERIREELRKGVTPQAAIRAGFDKALSAITDSNIT
                     TLIAGVVLWVLGTGPIRGFAIVL"
     gene            complement(1769..2098)
                     /gene="yajC"
                     /locus_tag="VI195_04210"
     CDS             complement(1769..2098)
                     /gene="yajC"
                     /locus_tag="VI195_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY6823617.1"
                     /translation="MNGLITDAWAQAGPGAGGGQFQFALLMAAFIALFYFMLIRPQQR
                     RAKEHQTLVAKLAAGDEVVTSGGLLGRITEVGDTFVTLEVADGVRVKVQKVQVTQLMP
                     KGTLKSA"
     gene            complement(2219..>2535)
                     /locus_tag="VI195_04215"
     CDS             complement(2219..>2535)
                     /locus_tag="VI195_04215"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY6823618.1"
                     /translation="RHARNGHLFTSTGVINIRNSAFAHDLTPLDAACDCYTCRHFSRA
                     YLRHLDRCNEILGSRLNTIHNLHFYIGLMRALRAAIAAGRLAEWSRVFLAQRAEQSAP
                     RG"
BASE COUNT          416 a          836 c          855 g          428 t
ORIGIN      
        1 agcacgatgg cgaagccgcg gatcgggccg gtgccgagca cccacagcac caccccggcg
       61 atcagcgtag tgatgttgga atctgtgatt gccgacagcg ccttgtcgaa gccggcgcgg
      121 atcgccgcct gcggcgtcac ccccttgcgc aactcctcgc ggatgcgctc gtagatcagc
      181 acattggcat ccaccgccat gccgacggtg agaatgatgc cagcgatccc cggcagcgac
      241 agcgaggcgc gcatcatcga gagcagcgcg gtgagcagca cgacgttggc gagcagcacc
      301 aggtcagcga ccagcccgaa ggtcttgtag tagatccaca tgaacacgaa tacgccgccc
      361 atgccgacga tcagcgcggt cacacctttg tcgatgttgt cgcgcccgag gctcggcccg
      421 atggcgcgct cctcgatggg gtagagcgga gccgcgagcg atccggactt gagcagcagc
      481 gcgagctcgc gcgcctcgcc cgcctgcagc ccggtgatct ggaagttgtt gctgaacacc
      541 ccgcgaatgg tggcgtcgtt gatcacctgc tcgtcggtca cctcgcggct cactttctta
      601 ccgttcactt cgctggtctc gcggtgcttc tcgatcagca ccacggccat gcgacggttg
      661 aggttggtgc gcgtggtgcg cagcatggcg tcgccggcgc gcgcatcgag ccggatgttg
      721 accgccggac cttcctgcga ctgcccgctg gtggcgttgg tgagctggtc gccggtggcg
      781 atcacctccc gcttgagcaa caccgggcgg ccgaagcgcg tatgggtata gagcttggag
      841 ccgagcggca cgcgcccggt ctgctgcgcc tcgtaggcgt tgttctgcat gtcctcgagg
      901 cggaactcga gcgtcgccac gcgcccgagg atgtccttca cctccgcgga gttctgcacg
      961 cccggcagct gcacgttgat gcgatcgacc ccctgacgct gcacgatcgg ctccgacacc
     1021 ccgagcccgt tgacgcggtt gcgcagcgtc gtcatgttct gctcgagcgc gtagtcacgg
     1081 cgttcgcgga tctgcgcgct ggtcatggtg gcggtgatcg ccgagccgct aggcaggctc
     1141 tgggtgctga ccgacagtcc ctgcagcgcg gtgccgagtg cgccgcgggc cgcgttggcg
     1201 tcggcttccg gcggcagcac cacgcgcacc gcatcggtag cggcaccgcc ggctgccagg
     1261 gtgctcacct ccttgaccgg gatatgggct gccacgagcg cgcgccgcgc atcctgggcg
     1321 tagccatcga gcgcctgagt gaccgccgca ttcacatcga cctggtagag caggtacagg
     1381 ccgccgcgca agtcgagtcc gagcggcatg ggacgcagcc cgagggcgcg cagcgccgcg
     1441 ggagtgcgcg gtgcaaacga tagtgccgtg cggtaggtgt cggcatattg cgcgttgacc
     1501 gcatcgcgcg ccgcgagctg gtcgggcacg ctggtgaagc gcaccatcag acgtccgctg
     1561 tcgatgtagc tcttgccata gtgcacgccg tgctgcgtga ggaaggcctc gagcgcacgc
     1621 tgcgcatcgg cgttgacggc ggtatggtcc ttgcgctcca cctgcagcgc cgggtcctcg
     1681 ccgaagatat tcggcaacgc gaacaccagc gccagcagca gcaccgccgc aacgagcagg
     1741 tatttccagc gcgggtactc gagcatcgtc aggcgctctt cagcgtgccc ttaggcatca
     1801 gctgcgtaac ctgcaccttc tgcaccttga cgcgcacgcc gtcggcgact tcgagcgtca
     1861 cgaaggtgtc gcctacctcg gtgatgcgcc cgagcagccc gccgctcgtc accacctcat
     1921 cgccggcggc aagcttcgcc accagcgtct gatgctcctt ggcgcgacgc tgctgcggac
     1981 gaatcagcat gaagtagaac agcgcgatga acgccgccat caacagcgcg aactggaact
     2041 gtccgccgcc agcgcccggg ccggcctgtg cccaggcgtc cgtgatcaag ccattcatgt
     2101 gtgtagtccc agcgacgaaa agggcggcat tatgccattc ccgatcgaaa ctcagttctc
     2161 aagagcaagc ttagcgtatc cgcagggcgc ccacgtggac cgaagaggtg gccgcgcgtc
     2221 aaccccgggg cgccgactgc tcggctcgct gtgcgagaaa gacccggctc cattcggcga
     2281 gccgccccgc ggcgatggcc gcgcgcagtg cacgcatcag gccgatgtag aaatgcaggt
     2341 tatggatggt gttgagccgt gagccgagga tctcgttgca gcggtcgaga tgccgcaggt
     2401 aggcacggct gaagtgccgg cacgtgtagc aatcgcaggc tgcatccagt ggtgtcaggt
     2461 cgtgggcgaa ggcgctgttg cggatgttga tgaccccggt ggaagtgaac aggtgcccgt
     2521 tgcgcgcgtg ccggg
//
LOCUS       DATDMP010000248         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_482553, whole genome shotgun sequence.
ACCESSION   DATDMP010000248 DATDMP010000000
VERSION     DATDMP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482553"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /gene="proA"
                     /locus_tag="VI195_04220"
     CDS             <1..516
                     /gene="proA"
                     /locus_tag="VI195_04220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY6823619.1"
                     /translation="LNAKMRRTGICGAAETLLIDRACVATHLAPVVRDLLEAGCEVRG
                     DATVRQVDPRVRPARESDFYTEYLDAIIAARVVDDVDAAIAHIAHYGSAHTESIVTEN
                     QATAEHFLRRVDSAIVLHNASTQFADGGEFGMGAEIGISTDRFHARGPVGIEQLTSYK
                     YVVRGTGQIRP"
     gene            complement(540..1022)
                     /locus_tag="VI195_04225"
     CDS             complement(540..1022)
                     /locus_tag="VI195_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY6823620.1"
                     /translation="MPAIPPFSFSRTPIEPAALRAALADPACGGYAAFEGWVRDHNQG
                     ERVRRLEYEAFEPLAIREAERIVAEAVARFGVAHAAGVHRIGDLGIGELAVWIGVSAP
                     HRDEAFRACRYIIDEVKQRLPIWKKEHYASGDSGWVNCERCAAGAGHAHRAPAPATHP
                     "
     gene            complement(1037..1897)
                     /locus_tag="VI195_04230"
     CDS             complement(1037..1897)
                     /locus_tag="VI195_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY6823621.1"
                     /translation="MSAPLYGLVLAGGRSSRMGQDKAALRYRGHSQLARAMALLAPHV
                     VRAFVSVRAEQRADPLRAEFAQIADTHENLGPIAGLLAAQERHPDRAWLVLACDLPLL
                     DEATLGHLLGARASGREATAYRSSRDGLPEPLCAVYEPRSHARLAAYIGRGRQCPRRF
                     LMEADVLLLDEPNPRALDNANTPEEHDSAMDALQQAAPGTDAPAGTTRRVQVRYYALL
                     REQAARSEEAVTTGARTPRELYAELAQRYRFSLPPEMLRVAINAEFGDWSAPLADGDS
                     VVFIPPVAGG"
     gene            complement(1894..2373)
                     /gene="moaC"
                     /locus_tag="VI195_04235"
                     /pseudo
     CDS             complement(1894..2373)
                     /gene="moaC"
                     /locus_tag="VI195_04235"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
     gene            complement(2392..>2637)
                     /locus_tag="VI195_04240"
     CDS             complement(2392..>2637)
                     /locus_tag="VI195_04240"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823622.1"
                     /translation="PFCGDCSRVRLSSDGVVYTCLFATHGTSVRDLLRGGASDDQLLE
                     FIAALWQRRADRYSELRSSLPRPHGEERKVEMFYIGG"
BASE COUNT          369 a          951 c          961 g          356 t
ORIGIN      
        1 ctcaacgcca agatgcgccg caccgggatc tgcggcgccg ccgagacgct gctcatcgac
       61 cgcgcgtgcg tcgccaccca cctcgcgccg gtggtgcgcg acctgctgga agccggctgc
      121 gaagtgcgcg gcgatgcgac ggtgcgccag gtcgacccgc gggtaaggcc ggcgcgcgag
      181 agcgatttct ataccgagta cctcgatgcc atcatcgccg cccgggtggt ggacgacgtc
      241 gacgcggcga tcgcgcacat cgcccactac ggctcggcgc acaccgagtc catcgtcacc
      301 gagaatcagg cgaccgcgga gcacttcctg cgccgggtcg acagtgcgat cgtgctgcac
      361 aacgcctcga cgcagtttgc cgacggcggg gagttcggca tgggagccga gatcggcatt
      421 tccaccgacc gcttccacgc gcgcggcccg gtcgggatcg agcagctgac gagctacaag
      481 tacgtggtgc gcggcaccgg ccagatcagg ccgtgaggcg gcgagcggcg cgccacctgc
      541 taaggatgcg tcgccggggc cggagcgcgg tgcgcgtggc ccgcgcccgc ggcgcagcgc
      601 tcgcagttca cccagccgga atcgccgctg gcgtaatgct ccttcttcca gatcggcagc
      661 cgctgcttga cctcgtcgat gatgtagcgg caggcgcgga acgcctcgtc gcggtgcggc
      721 gcactcacgc cgatccagac cgcgagctcc ccgatgccga gatccccgat gcggtgcacg
      781 ccggccgcat gcgccacgcc gaagcgcgcc accgcctcgg cgacgatgcg ctcggcctcg
      841 cggatcgcga gcggctcgaa ggcctcgtac tccagccgcc gcacgcgctc gccctggttg
      901 tgatcgcgca cccagccctc gaaggcggca tagccgccgc acgccggatc agcgagtgcc
      961 gcccgcagcg ccgcgggctc gatcggcgtg cggctgaagc tgaacggcgg gatcgcgggc
     1021 atgcgcactc gccgcctcag ccgcccgcaa ccggcgggat gaacaccacc gagtcgccgt
     1081 cggcgagcgg cgccgaccag tcgccgaact cggcgttgat ggcgacccgc agcatctccg
     1141 gcgggagcga aaagcggtag cgctgcgcga gctcggcgta cagctcccgc ggcgtgcgtg
     1201 cgccggtcgt cacggcctcc tcgctgcgcg cggcctgctc gcgcagcagc gcgtagtagc
     1261 gcacctgcac gcgccgggtg gtgcctgcgg gggcgtcggt gcccggcgcg gcttgctgga
     1321 gtgcgtccat ggcggagtca tgttcctcgg gagtgttggc gttgtcgagg gcgcgcggat
     1381 tcggctcatc gagcagcagc acgtcggctt ccatgaggaa gcggcgcggg cattgcctgc
     1441 cgcggccgat gtaggcggcg aggcgggcgt ggctgcgcgg ctcgtacacc gcacacaagg
     1501 gttcgggcag tccgtcgcgg ctcgagcggt aggcggtcgc ttcgcgtccg ctggcgcgcg
     1561 cgccaagcag gtgcccgagc gttgcctcat cgagcagcgg caggtcgcac gccagcacca
     1621 gccaggcgcg gtcggggtgg cgctcctggg cggcgagcag gccggcgatc ggaccgaggt
     1681 tctcgtgcgt gtcggcgatc tgcgcgaact ccgcgcgcag cggatcggca cgctgctcgg
     1741 cgcgcaccga gacgaaagcg cgcaccacgt gcggggcgag cagcgccatg gcacgcgcga
     1801 gctggctgtg gccccggtag cgcagcgccg ccttgtcctg gcccatgcgg ctgctgcgcc
     1861 cgccggccag caccaggccg tagagcggtg cgctcacggc gtgccgcgcg cggcggaacg
     1921 cgcgagccgc cgccgtcctc cggacttctc cagcagccgc accgcggtga gctcgatgtc
     1981 gtgcgagagc gccttgcaca tgtcgtagat cgtcaatgcc gcgacggtgg cgccggtgag
     2041 cgcctccatt tccacgccgg tgcggtgatg caccgccgcc cggcagcgta tcacgagcac
     2101 gcccgccggc ccgtgccgca cctcgatccg gcagtcctcg agtgccagcg ggtgacagaa
     2161 cgggatcagc tcgtgggtgc gcttggccgc catgacgccg gcgatgatgg cggtgtcgaa
     2221 caccggaccc ttggcggtac ggtggccgct cgcacgcagc gcgcgcgcca ccgtgcgcgg
     2281 caggcgcaga cgcgcctccg ccaccgcgac gcgctgcgtc acttccttgc cgccgacatc
     2341 caccatggcc ggcgtgttgc gcgcgtcgag gtgcgagagc cgcgcgcgcc atcagccacc
     2401 gatgtagaac atctcgacct tgcgctcctc gccgtggggg cggggcaggc tcgagcgcag
     2461 ctcgctgtag cggtcggcgc gccgctgcca cagtgccgcg atgaattcga gcagctgatc
     2521 gtcgctggca ccgccgcgca gcaggtcgcg cacgctggtg ccgtgggtgg cgaacaggca
     2581 ggtgtacacc actccgtccg acgacagtcg cacccgcgag cagtcgccgc agaaggg
//
LOCUS       DATDMP010000249         2041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_705502, whole genome shotgun sequence.
ACCESSION   DATDMP010000249 DATDMP010000000
VERSION     DATDMP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705502"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VI195_04245"
     CDS             <1..1134
                     /locus_tag="VI195_04245"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEY6823623.1"
                     /translation="VAAAAQALASGNTFGSAVQIGTAAGQQAAAATGGNLSAATAQQY
                     ATIGANTLLAGGNVNAQAANLATSEYSKTQGFYEQFPQDIKMFGLSFNSQVQSTGTAL
                     QGEVAYRHNVPLQIDDVELIYASLTPFETGLANLLHEPVTGPGHCVPNSATPITGCNQ
                     LGSYYKTDAQGRVISQTVQGYELKDTYHFDLTATQVFANVFKASQAVLIFEAGADYVP
                     GLEDKLSGGPQGFGLRFDGPGTNLSGNPNLGGYPEFPAVSGQCVKGPVPNPLGQCLEP
                     GAAFATTWAWGYVIAGRLEYNNAIGDWNLLPHFTWQQDVTGVSPGPGGAFRAGRIAAT
                     LGLTASVRNMLELDVSYTNYGGAGQYNLLNDRDFIAASVKFSF"
     gene            1181..>2041
                     /locus_tag="VI195_04250"
     CDS             1181..>2041
                     /locus_tag="VI195_04250"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEY6823624.1"
                     /translation="MKMLLRSAAAGVLLASVAFGAVSPQEAARLGQDLTPLGGEKAGN
                     ADGSIPAWTGGLKSPADAGFPNYRNGEHYPDPYASDKPLFTITSANLAQYAAKLSEGH
                     KALFKTYPDYKLIVYPTHRSGASPERVYEATKRNATTAKLVPDGNGFTGALGGTPFPI
                     PQSGVEVYWNHVTRWRGVVAALDVGQAPMTAGGGYTLVNFKEEFYFQYYEPGMTEAAL
                     NNILLYFTQETTAPARLAGEVLLVQETIDQAREARRAWVYNPGQRRVRRAPNVAFDNP
                     GTNADNLRTAD"
BASE COUNT          373 a          695 c          647 g          326 t
ORIGIN      
        1 gtcgctgccg ccgcacaggc gctcgcttcg ggcaatacgt ttggctccgc cgtgcagatc
       61 ggcaccgcgg ccggccagca agcggccgcg gccaccggcg gcaatctcag tgccgccacg
      121 gcgcagcagt acgccaccat cggcgcgaac accctgctcg ccggcggcaa tgtcaacgcc
      181 caggcggcga atcttgcgac ttccgagtac agcaagaccc agggcttcta cgaacaattc
      241 ccgcaagaca tcaagatgtt cgggctgtcc ttcaactcgc aggtacagag cacgggcact
      301 gcgctgcagg gcgaggtggc gtaccggcac aacgtgccgc tgcagatcga tgatgtcgag
      361 ctcatctacg cttcgctcac gcccttcgag accgggctcg ccaaccttct gcatgagccc
      421 gtgaccggcc ccgggcactg tgtgcccaat tcggcgacgc ccatcaccgg atgcaaccag
      481 ctcggctcgt actacaagac cgatgcacag gggcgcgtga tctcgcagac cgtccagggc
      541 tatgagctca aggacaccta ccacttcgat ctcaccgcaa cgcaggtgtt tgccaatgtc
      601 ttcaaggcat cgcaggcggt gctcatcttc gaggcgggcg ctgactacgt tccgggcctc
      661 gaggacaagc tcagcggcgg tccccagggc ttcggcctgc gcttcgatgg gccgggcacc
      721 aatctcagcg gcaatccgaa cctcggcggc tatccggagt ttccggcggt cagcggccag
      781 tgcgtcaagg gcccggtccc gaatcccctc ggtcaatgcc tcgagcccgg cgcggcgttc
      841 gcaaccacct gggcatgggg ctacgtgatc gccggacggc tcgaatacaa caacgcgatc
      901 ggggactgga acctgctgcc gcacttcacc tggcagcagg atgtgaccgg tgtgtcgccc
      961 ggtccgggcg gagcgttccg cgcgggccgc attgcggcca ccctaggact cacggccagc
     1021 gttcgcaaca tgttggagct cgacgtctcc tataccaact acggcggcgc cggccagtac
     1081 aacctgctga acgatcgtga tttcatcgcg gcgagtgtga aattctcgtt ttgaacgtgc
     1141 gcgcgcggtt gcgctggaca cgcacgatgg agggagcgca atgaaaatgc tgcttcggtc
     1201 tgccgccgcg ggagtgctgc tcgcatccgt ggccttcggc gcggtgagtc cgcaggaggc
     1261 cgcgcggctc ggtcaggacc tcacgccgct cggcggggag aaggccggca acgcggacgg
     1321 gagcattccg gcgtggaccg gaggattgaa gtcccccgcg gatgccggtt ttcccaacta
     1381 tcgcaacggc gagcactacc ccgacccgta cgcgagcgac aaaccacttt ttaccatcac
     1441 ctcggcgaac cttgcgcagt acgccgccaa gctcagcgag ggacacaagg cgctgttcaa
     1501 gacttatccc gactacaagt tgatcgttta cccgacacac cgcagcggcg catccccgga
     1561 gcgggtgtac gaggccacca agcgcaatgc gacgacggcg aagctggtgc ccgatggcaa
     1621 cggcttcacc ggcgcgctcg gcggtacgcc gtttccgatt ccgcagagcg gcgtcgaggt
     1681 ctactggaac cacgtgacgc gctggcgcgg agtagtggcc gcgctcgacg tgggccaggc
     1741 accgatgacc gccggtggcg gctacacgct cgtgaacttc aaggaggagt tctacttcca
     1801 gtactacgag cccgggatga ccgaggcggc actcaacaac atcctgctgt acttcacgca
     1861 ggagaccacg gctccggccc gtctcgccgg tgaggtgctg ctggtgcagg agaccatcga
     1921 tcaggcgcgc gaggcgcgcc gtgcctgggt ctacaatccc ggccagcgcc gcgtgcggcg
     1981 cgccccgaat gtcgccttcg acaatcccgg caccaacgcg gacaacctgc gcaccgcgga
     2041 c
//
LOCUS       DATDMP010000250         3003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_38822, whole genome shotgun sequence.
ACCESSION   DATDMP010000250 DATDMP010000000
VERSION     DATDMP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38822"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VI195_04255"
     CDS             <1..551
                     /locus_tag="VI195_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY6823625.1"
                     /translation="GQLWTQGLLNVVPSSVLSVQILTNNIVVSLFAYCLGFLFGLGTL
                     YILGLNGLMLGAIFAFVGAHGLAGALFRFIVAHGCVELSVMCLSGAAGAAIGEALIRP
                     GAGGRLESFRSAALRTAPVLLACAVLLVGSGLIEGFISPDPRFSLLSRVVVGVGYWCL
                     MVAFLSGRLFGRHAGNERSAPA"
     gene            complement(548..1843)
                     /locus_tag="VI195_04260"
     CDS             complement(548..1843)
                     /locus_tag="VI195_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY6823626.1"
                     /translation="MHLARRSYLLVMLTAVIAIAGIWSSEHALARLWHVPAALLLLGL
                     AVEGLAVRRLTPGTGLHTASPAFLGRLQRGAFVFANDSARTLALEYAPATPAGFETAA
                     EVRRLTLPARTTCEDALPLLPVRLGPQTWPQLPARLRGPLALAWWNLALTPQAKCLVA
                     PDTLRGSARVRGLAAGARARRSAGAGQELYKLRGYARGDPLTRIDWKATARTGQLISR
                     EYSEDQHLDIFIAIDAGRLSRVRAGALDRLGLYSNAAARLAEIVTHNDDRIGLIVYAE
                     RVLASCPPARGLAAVARLRSALAGLAAQGAESDPIVAAITIRKLLRQRALIVLLTDLD
                     DANIAAALARAVRLLAPPHLVLVAGVHSGEIETLARARAREWQDPWIALAAAEHEERA
                     ARQRLLLQRLGAPVVAAAAQLLESTLFEHYEALRRSRRI"
     gene            complement(1855..2805)
                     /locus_tag="VI195_04265"
     CDS             complement(1855..2805)
                     /locus_tag="VI195_04265"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6823627.1"
                     /translation="MSNAIRDLRELLTQELGRVVIGVETSVDALVIALIARGHVLVQG
                     VPGLGKTLFAKALARALGGEFKRIQGTADLMPSDIIGVHVFDEAARNFAFRPGPLFAD
                     VVLVDEINRAGPKTQSALLEAMEERQVSVERSVLPLPADFIVLATQNPREFEGTFPLP
                     ESQLDRFMLRIDMSYPARAAETHILARYGGVLAGAHAALDSVAVVDRALLGEARAAAE
                     NIHVSEALSGYLLDLAAASRTHPRLALGLSTRGVLALLKAARVVAGMRGSDFVTPDDV
                     KQVAPWVLAHRLVLTPEATLEGVADAQLVQGLLASTPVPR"
     gene            complement(2802..>3003)
                     /locus_tag="VI195_04270"
     CDS             complement(2802..>3003)
                     /locus_tag="VI195_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823628.1"
                     /translation="ERLRHATGRSSSDPARLWEWLESHPRLARAEVLQLRAWHAAAYA
                     NRRVPLIRLHNLIVNTERQLAQ"
BASE COUNT          443 a         1066 c         1034 g          460 t
ORIGIN      
        1 gtggccagct gtggacgcag ggtctgctca acgtcgtgcc ctcttcggta ctgtcggtgc
       61 agatcctgac caacaacatc gtggtgagcc tgttcgcgta ttgcctggga tttctcttcg
      121 ggctcggcac actctacata ctcggtctca atggactgat gctcggcgct atcttcgctt
      181 tcgtcggcgc gcacggcctc gccggcgcgc tgttccgctt catcgtcgcc cacggctgcg
      241 tggagctttc ggtaatgtgc ttgtcgggtg ccgcgggcgc ggccatcggc gaggcgttga
      301 tccggcccgg cgcgggcggc cgactcgagt cgttccgcag tgccgcgttg cgcaccgctc
      361 cggtcctgct cgcgtgcgcc gtgctgctgg tgggctcggg actgatcgag gggttcatct
      421 cgcccgatcc ccgtttctcg ctgctcagcc gtgtcgtagt cggggtcggc tactggtgcc
      481 tcatggtcgc gttcctcagc gggcgactct tcggccggca cgcaggcaac gagcggagcg
      541 ctcccgccta gatgcgccgc gaccgtcgca gcgcctcgta gtgctcgaac agggtgctct
      601 ccagcagctg cgccgcagcc gccactaccg gtgcgccgag ccgctgcagc agcaggcgtt
      661 gccgggcggc gcgctcctcg tgctcagcgg ctgcgagtgc aatccacgga tcctgccact
      721 cgcgcgcgcg cgcgcgcgcc agcgtctcga tctcgccgct gtgcacaccc gcgaccagca
      781 ccagatgcgg tggtgcgagc agacgcaccg cgcgggcgag cgccgcggcg atgttggcat
      841 cgtcgagatc cgtcagcagc acgatgaggg cgcgctgccg caggagcttg cgaatggtga
      901 tcgcggccac gatggggtcg gactcagcgc cctgtgccgc gagccctgcc agcgcgctgc
      961 gcagtcgcgc caccgcggcg agcccacgcg ccggcgggca gctggcgagc acccgctctg
     1021 cgtacacgat cagtccgata cgatcgtcgt tgtgggtgac gatctcggcg aggcgcgccg
     1081 cggcgttgct gtagagcccg agacggtcga gcgcgccggc tcgcacccgg ctcaagcggc
     1141 cggcgtcgat ggcgatgaag atatcgagat gctgatcctc gctgtactcg cggctgatga
     1201 gctggccggt gcgcgcggtc gccttccagt cgatgcgcgt caatggatcg ccgcgggcgt
     1261 agccgcgcag cttatagagc tcctggccgg cgccggcaga gcggcgcgcg cgcgcgccgg
     1321 ccgcgagccc acggacgcgc gcactgccac gcagggtgtc gggtgccacc aggcactttg
     1381 cctgcggcgt gagtgccagg ttccaccagg cgagcgcgag cgggccgcgt aaccgcgccg
     1441 gcagctgcgg ccaggtctgc gggccaagcc gcaccggcag cagcggcagc gcatcctcac
     1501 aggtcgtgcg cgccggcagt gtgagccggc gtacctccgc ggcagtctcg aagcctgccg
     1561 gggttgcggg cgcatactcg agcgcgagcg tgcgcgcgct gtcgttggcg aacacgaacg
     1621 cgccacgctg cagcctgccg agaaaggcgg gcgaagcggt atggagaccc gtgccgggcg
     1681 tgaggcggcg caccgccagc ccctcgaccg ccagaccgag cagcagcagc gccgcaggca
     1741 cgtgccacag ccgcgccaac gcatgctcgc tcgaccagat gccggcgatc gcgatcaccg
     1801 cggtcagcat caccaacaga tagctgcgcc gtgccagatg caagcgcgac tccttcagcg
     1861 cggcaccggt gtgctcgcga gcagaccctg caccagctgc gcatcggcga caccttccag
     1921 ggtcgcttcc ggtgtcaaca ccagccggtg cgccagcacc catggagcca cctgtttgac
     1981 gtcgtcgggc gtcacgaagt cgctcccgcg catacctgcg accacgcgcg ccgccttgag
     2041 cagcgccaac acgccacgcg tcgacagccc gagcgcgagg cgcgggtgcg tacggctagc
     2101 ggccgccaga tcgagcagat agccgctgag cgcctcggag acgtggatgt tctccgccgc
     2161 ggcgcgcgcc tccccgagca gggcacgatc gaccaccgcc accgaatcga gcgcggcatg
     2221 cgcgcccgcc aacactccgc cgtagcgcgc gaggatgtga gtctcggccg cgcgcgccgg
     2281 ataactcatg tcgatgcgca gcatgaagcg gtccagctgc gactcgggca gcgggaaggt
     2341 tccctcgaac tcgcgcggat tctgggtcgc cagcacgatg aaatccgccg gcagcggcag
     2401 cacgctgcgc tcgacgctca cctgccgctc ctccatcgct tccagcagcg ccgactgcgt
     2461 cttgggtccg gcacgattga tctcgtccac gagcaccaca tcggcgaaca gcggaccggg
     2521 gcggaacgcg aagttgcgcg ccgcctcgtc gaacacgtgc acgccgatga tgtcgctcgg
     2581 catcaggtcg gccgttccct ggatgcgctt gaattctcca ccgagggcgc gtgccagcgc
     2641 cttggcgaac agggtcttgc cgagcccggg caccccctgc accagcacat ggccgcgcgc
     2701 gatgagcgca atgaccaggg catcgaccga cgtttcgacc ccgatgacga cccggccgag
     2761 ctcctgcgtg agcagctcgc gaaggtcgcg gatcgcattg ctcattgcgc gagttgcctt
     2821 tcagtattaa cgatgaggtt gtgcagacgg atgagcggta cgcgccggtt cgcgtacgca
     2881 gcggcgtgcc aggcacgcag ctgcagcacc tccgcgcgcg cgagccgcgg atgactctcg
     2941 agccattccc atagccgcgc ggggtccgag ctgctgcggc cggtcgcatg gcgcagccgc
     3001 tcg
//
LOCUS       DATDMP010000251         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_38907, whole genome shotgun sequence.
ACCESSION   DATDMP010000251 DATDMP010000000
VERSION     DATDMP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38907"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..375)
                     /locus_tag="VI195_04275"
     CDS             complement(28..375)
                     /locus_tag="VI195_04275"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6823629.1"
                     /translation="MSYMLLIVEPQGQRTTRAPQQGRDLYQRMLEYGDSLKARGLLLA
                     SDSLRAPAARLSIRAGRQSVIDGPFTESKELVGGFFLLDCATREQALSLAAECPAAEW
                     ATIEVREVGPCYE"
     gene            complement(375..1646)
                     /locus_tag="VI195_04280"
     CDS             complement(375..1646)
                     /locus_tag="VI195_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6823630.1"
                     /translation="MTATDTHRTIDAVWRIESARLIAVLTRLLRDVALAEELAQDALV
                     TALEQWPRTGIPDNPGAWLMATAKHRAIDHLRRQRMLSRKHQALGHELQMEQLSDGDS
                     LEHSADDLVGDDLLRLMFIACHPLLSTEARTALTLKLLAGLTTAEIARAFLVPEATIA
                     QRIVRAKRTLAAARVPFEALPPEQLRERLSSVMEVIYLVFNEGYAATAGDDWMRQAMC
                     EEALRLGRMLAELAPTEPEVHGLVALMEIQASRLAARIGPNGEPILLMQQNRALWDPL
                     LIHRGLLALARGEALGATRGHYLLQAAIAACHARATSATDTDWVLITALYDELARLSP
                     SPVVELNRAVAVSMAYGPAAGLELTDQLAAQGVLRSYHLLPSVRGDLLARLGRYHEAR
                     GEFERAATLTQNRRESRLLLDRAAACERGES"
     gene            1832..3046
                     /gene="nhaA"
                     /locus_tag="VI195_04285"
     CDS             1832..3046
                     /gene="nhaA"
                     /locus_tag="VI195_04285"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY6823631.1"
                     /translation="MSTPHASRRRLWPLRFIGIEAVSGIVLLAAAAIALVCANSPLAH
                     AYESLWRYPLRLGTPLLGVRDLHFWVNDGLMSIFFLLVGLEIRREIHDGTLSDPRVAT
                     LPIIAAAGGVVVPALLYMLVAHGPEVGRGWAIPTATDIAFAVGVLSLIGRVPAALRML
                     LLTLAIADDVAAILIIAFFYSGGLVIAQFLLVAAGAALVLLLQALGVRTALAYVFPGA
                     VLWLGMLRAGVHPALAGVVLGLMTPVTSEFGLLKRQRALTSRSEAPVVRVEAMLHPYV
                     AFGIMPLFAFANAGVPLAGLELSAGAPLRVGAGIVLGLVLGKPLGIVLAALAAVKLKV
                     AELPASIHWSQLGLLGLLGGIGFTMSVFIANLAFGDPALLTAAKFAVLVGSAIAATLG
                     FIHGRLTSSAAE"
BASE COUNT          478 a         1093 c         1063 g          590 t
ORIGIN      
        1 atacccgcgc ttccttcact catcgcttca ctcgtagcag ggtccgacct cgcgcacttc
       61 gattgtggcc cactcggccg ccgggcactc ggcagcgagg ctcagcgcct gctcgcgcgt
      121 cgcgcagtcg agcaggaaaa atccgcccac cagctccttc gattcggtga aaggtccgtc
      181 gatcacactc tgccgtcctg cgcggatgct gagacgcgcc gcgggcgcgc gcagcgagtc
      241 gctcgcgagc agcaggccgc gcgccttgag cgaatcgccg tattcgagca tgcgctggta
      301 gaggtcacgc ccctgttgcg gcgcgcgggt ggtgcgctgc ccctggggct cgacgatcag
      361 cagcatgtac gacatcagct ctctccgcgc tcgcaggcgg ccgcgcgatc gagcagcaag
      421 cggctctcgc gtctgttctg ggtcagggtg gccgcgcgct cgaattcccc gcgcgcctcg
      481 tggtaccggc cgagcctggc gagcaggtcg ccgcgcacgc tcggcagcag atgataggag
      541 cgcagcacac cctgcgccgc gagctggtcg gtgagctcga ggccggctgc cgggccgtag
      601 gccatggaga ccgccacggc acgattgagc tccaccaccg gtgagggtga aaggcgtgcc
      661 agctcatcgt agagcgcggt gatgagtacc cagtcggtat cggtggcact cgtcgcacgc
      721 gcgtggcaag cggcgatcgc cgcctgcagc aggtagtgcc cgcgcgtcgc gccgagtgcc
      781 tcgccgcgcg cgagcgccaa cagtccgcgg tgaatcagca gcggatccca gagcgcgcgg
      841 ttctgctgca tcagcaggat cggttcgccg ttggggccga tgcgcgccgc gagccgggag
      901 gcctggatct ccatgagcgc caccaggccg tgcacttccg gctccgttgg cgcgagctcg
      961 gcgagcatgc gtcccagccg cagcgcttcc tcgcacattg cctggcgcat ccagtcatca
     1021 cccgcggtcg ccgcatagcc ctcgttgaat accaggtaga tgacctccat gacggaggac
     1081 agacgctcgc gcagctgctc gggcggtagc gcctcgaagg gcacgcgcgc tgcggcgagc
     1141 gtgcgcttgg cgcgcacgat gcgctgcgca atcgtggcct ccggcaccag gaaggcgcgc
     1201 gcgatctctg cggtcgtgag tccggccagc agcttcagcg tgagcgccgt gcgcgcctcg
     1261 gtcgagagca gcggatgaca ggcgatgaac atgagccgca gcaggtcatc accgaccaga
     1321 tcgtcggcgc tgtgctcgag cgagtcgccg tcactcagct gctccatctg gagctcgtgg
     1381 ccgagtgcct ggtgcttgcg cgacagcatg cgctggcggc gcaggtgatc gatcgcgcgg
     1441 tgtttcgcgg tcgccatcaa ccaggcaccg ggattgtcgg gaatacccgt gcgcggccac
     1501 tgctcgagcg cggtgaccag cgcatcctgc gccagctcct cggcgagtgc gacatcgcgc
     1561 agcaatcgcg tgagcacggc gatgagccgc gcggactcga tgcgccacac ggcatcgatc
     1621 gtgcgatgag tgtccgtagc cgtcatgact atcgatgaca ccatcgccac aggaggagcg
     1681 caagcaaagc tcgctcaact gttttcgcca agcgacagaa tgggaccgct tactcgcatg
     1741 cccatacgac gaagcggacc gcctggcatc gacatgcgcg cgcctaattt ttcgtcctag
     1801 gctaagctgc tgaaagtccg ggagtttgcc agtgagcacg ccgcacgcca gccgccgcag
     1861 actctggcca ttgcgcttca tcggtatcga ggcggttagc ggaatagtgc tgctcgccgc
     1921 cgcggccatc gcgctcgtct gcgcgaactc gccgctcgcg cacgcctacg aatcgctgtg
     1981 gcgctacccg cttcgcctcg gtacgccgct tctcggggta cgcgatctgc acttctgggt
     2041 gaacgatggg ctgatgagca tttttttcct gctcgtgggt ctggagatac gccgggaaat
     2101 acacgacggg accctttcgg atccacgcgt cgcgacgctt ccgatcatcg ctgccgccgg
     2161 cggagtggtc gtgccggcac tcttatatat gctggtggcg cacggccctg aggtcgggcg
     2221 cggctgggcg atcccgactg ccaccgacat tgccttcgcc gttggagtgc tgtcgctcat
     2281 cggccgcgtg cccgcggcgc tgcgcatgct gctgctgacg ctcgccatcg ccgatgacgt
     2341 cgcggcgatt ctcatcatcg ccttcttcta ttccggcggg ctcgtcatcg ctcagttcct
     2401 gctggtcgcc gcgggcgccg cgctggttct gctcctgcag gcgctcggcg tgcgtacggc
     2461 actcgcttat gtatttccag gggcggtgct ctggctcggc atgctgcgcg ccggcgtcca
     2521 ccccgcgctg gccggcgtcg tgctcgggct catgacgccg gtgacctccg aattcgggct
     2581 cctcaagcgg caacgcgcgc tcacatcgcg ctccgaagcc cccgtggtgc gcgtcgaagc
     2641 catgctgcac ccgtacgtcg ccttcggcat catgccgctg ttcgctttcg ccaacgcggg
     2701 cgtaccgctc gcgggtctcg agctttctgc tggcgcgccc ctcagggtcg gcgctggcat
     2761 tgtgctggga ctggtgctgg gcaaaccgct ggggatcgtg ctggcggcgc tcgctgcggt
     2821 gaaattgaag gtcgccgagc tgcccgcgag catccactgg tctcaactgg ggctgcttgg
     2881 ccttctgggc ggaatcggct tcaccatgtc ggtcttcatc gccaacctgg cgttcggtga
     2941 cccggcactc ctcacagcgg cgaaatttgc cgtgctggtc ggctccgcca tcgccgcgac
     3001 gctcggtttc atccacggcc gcctcacctc gtccgcggcg gagtgagctt ccggtagatt
     3061 ggctgcggat acgcacggca aagccaaagc ttgcgtacct ctaacccgaa gcttgagcgg
     3121 tgccgtaaag attatgttat ctatagtcgc gattgggtac ctgatcgtcc gcgtgcgacg
     3181 cctgccgcgc ttgccgaaaa gcgtgttgca gcccaacaat gcgt
//
LOCUS       DATDMP010000252         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_594501, whole genome shotgun sequence.
ACCESSION   DATDMP010000252 DATDMP010000000
VERSION     DATDMP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594501"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VI195_04290"
     CDS             complement(<1..362)
                     /locus_tag="VI195_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823632.1"
                     /translation="MALLIWSATNASSCSASGGWAGDQPSSGSVSTGALSGTTSYTLS
                     CTGPGGRASRSVQVSVSKVALNGPSCSASSGGLTLRASAARKSGESPLLMFFDATGTT
                     SSSVANNSSAFQDIAYRW"
     gene            complement(1096..1707)
                     /gene="wrbA"
                     /locus_tag="VI195_04295"
     CDS             complement(1096..1707)
                     /gene="wrbA"
                     /locus_tag="VI195_04295"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY6823633.1"
                     /translation="MAKVLVVYYSSYGHIEAMAYAQAEGAARVPNAQVTVKRVAELVA
                     ADVAKASGFKLDQAAPLASPEELEQYDAILFGTPTRFGNMAAQMRNFLDQTGAQWMRG
                     ALVGKVGSVFCSTASQHGGQETTITSFHLTLLHHGMIIVGLPYTFKDLATMREVTGGT
                     PYGASCVTGAGNELRMPTALELEMCRYQGEHVVRIASRLARAG"
     gene            complement(1718..>2405)
                     /locus_tag="VI195_04300"
     CDS             complement(1718..>2405)
                     /locus_tag="VI195_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6823634.1"
                     /translation="PQVSALRATFRLIRLDRRGHGLSGGVATPQRDGEDLAALCEHLG
                     LSRVALLGMSQGVRGVLEFACAAPRRVSALILDGPPALATAADAEVPLQRYAALVRTH
                     GIDAFRSEWARHALMQLRTRDPQVCALLAAMIARYPGTDLTQPSLRAELAGAHLRLQR
                     LRVPALVLSGEYDLPARRQAAKQLAAQLPDAELAVIPTAGHLPNLDSPDTYSKLCRGF
                     LTRHCPGDIS"
BASE COUNT          430 a          768 c          786 g          421 t
ORIGIN      
        1 tcccagcggt aggcgatatc ctggaatgcg ctgctgttat tcgccaccga actgctggta
       61 gtgccggtgg cgtcaaagaa catgagcaac ggcgattccc ccgacttgcg agccgcgctg
      121 gcgcgcagcg tcagtccgcc actgcttgcg ctgcaggaag gtccattgag agctaccttg
      181 gaaacactca cctgcaccga gcggctagcg cggccgccgg ggccggtgca gcttagtgtg
      241 tagctggtgg taccgctcag cgcgccagtg ctgacactgc cgctcgaggg ctgatcaccc
      301 gcccagccgc ccgaggcgct gcaactgctc gcattggtcg cagaccagat cagcagcgcc
      361 attccgccgc tgctcacgct ggtggggctg gcgctcagaa agatcgtggg accggtcgat
      421 gctgcacccg gcagcccttg ttccggcgtt ggacctccgg cggatccctg actgttctca
      481 gtgatgccgt catcgcaacc gctcgctagc gcaatcagta cgcagcacag cgcggtgcgc
      541 aggactgctg gaggagggcg aatgcggata tccgcaaggc gcactgaatg acgtccttct
      601 ataggttgcg cggcttcgct ggaatcgcga acgagctcga agacattgca tcgtcgcctt
      661 cggccgattg ccgagaacaa cacaaacgca atcaaacacg agctgccaga cagatataaa
      721 tagtatcccg cagcgctgta gctaactcgc cactagaacg atctgtgact aacctcacga
      781 cggcccgatt taccttatga cagccaccgg cgcagctggc cgcgcgacgg cgctgcggcc
      841 gccgcgccgc tcgggctttc gacataagct tgtggcgtgt gctcgtcagt cggcgtgcag
      901 cagctgtgcg caggttgcgc ccttgtgcgg aatgcggccg cgcgatacag ggcgcgtgga
      961 gtcgccgatt ctaactaccc aacatcagcg gttacgccgg tcatgaagac agcgcgggcg
     1021 gcgctcactg cgtgcggaag agatatgtgc tgcgctggct cagcgagctc gcagcgggag
     1081 tcgcgctgtc aatgctcagc cggcgcgtgc cagacggctc gcgattcgca ccacgtgctc
     1141 gccctgatag cggcacattt ccagctcgag cgccgttggc atgcgcagct cgttgcccgc
     1201 acccgttacg cagctcgcac cgtacggggt accgccggtg acctcgcgca tcgtcgcgag
     1261 atccttgaac gtgtagggca gtcccacgat gatcatgcca tgatgcagca gggtgaggtg
     1321 aaacgaggtg atcgtcgttt cctggccgcc atgttggctg gcggtactgc agaacacact
     1381 gccaaccttg ccgacgagcg ccccgcgcat ccactgcgca ccggtctggt cgaggaagtt
     1441 gcgcatctgt gcggccatgt tgccgaagcg tgtcggggtg ccgaacagga ttgcatcgta
     1501 ctgctcgagc tcttccgggc tggctagcgg ggcggcctga tcgagcttga agcccgaggc
     1561 cttggccaca tccgctgcaa ctagttccgc gacgcgtttc accgtgactt gtgcattggg
     1621 cacgcgggcg gctccctcgg cctgcgcata cgccatcgct tcgatatgac cgtaactgct
     1681 gtagtagaca acgagaacct tggccatggg cactccttca ggagatatcg ccggggcagt
     1741 gacgggtgag aaacccgcga cagagtttgc tgtaggtatc gggactatcc agattcggca
     1801 aatgacccgc tgtgggaatc accgcgagct cggcatccgg cagttgtgcc gcgagctgct
     1861 tggcagcctg ccggcgggcg gggagatcgt actcgccgct caatacgagc gcaggcaccc
     1921 gcagccgctg caggcgcagg tgcgcgccgg cgagttcggc tcgcagtgac ggctgggtga
     1981 gatccgtgcc cgggtagcgc gcgatcatgg cagcgagcag tgcgcacact tgaggatccc
     2041 gcgtgcgcaa ttgcatcaat gcatggcgtg cccattctga gcggaatgcg tcgataccgt
     2101 gcgtgcgcac cagcgccgca tagcgctgca gcggcacttc cgcgtccgct gcggtggcga
     2161 gcgccggtgg cccatcgaga atgagtgcgc tcacccggcg cggcgcggcg caggcgaatt
     2221 cgagtacccc ccgcacgcct tgcgacatgc cgagcaacgc gacccggctg agtcccaggt
     2281 gctcgcacaa ggcggcgaga tcctcgccat ctcgctgcgg agtagcaacg ccgccggaga
     2341 gaccatggcc gcggcgatcg aggcgtatga ggcgaaacgt ggcacgcagc gcgctcacct
     2401 gcggc
//
LOCUS       DATDMP010000253         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1594267, whole genome shotgun sequence.
ACCESSION   DATDMP010000253 DATDMP010000000
VERSION     DATDMP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594267"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VI195_04305"
     CDS             complement(<1..242)
                     /locus_tag="VI195_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823635.1"
                     /translation="MPRASVNGVVLHAGDETLSAEALRQRACTELLRQAAQAAGLLDA
                     SDTASADGVISEAASRAIEALLEQALQLPEPAEDALR"
     gene            complement(235..972)
                     /gene="narI"
                     /locus_tag="VI195_04310"
     CDS             complement(235..972)
                     /gene="narI"
                     /locus_tag="VI195_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY6823636.1"
                     /translation="MSTLENYLHVFFFGSYPYICMVVFLMGSLARFDRDQYTWKSDSS
                     QMLRTGTLRWGSNLFHAGVLFLFIGHFVGLLTPHWVYEPFISVAHKQQLAVYAGGVAG
                     FLCFVGLSLLIHRRIFDARIRLTSHRTDLAILIILWVQLTLGLITLPFSLSHSDGSVM
                     LVLADWAQRVMTFRVGGAEGLIPIAWPYKVHIVLGMTIFLLFPFSRLVHVWSGFATIA
                     YLFRPQQVVRSRRLNLPEGQNQPRVRA"
     gene            complement(969..1634)
                     /gene="narJ"
                     /locus_tag="VI195_04315"
     CDS             complement(969..1634)
                     /gene="narJ"
                     /locus_tag="VI195_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEY6823637.1"
                     /translation="MQKTLRVLSALLGYPDAQMRSHLPEMREILRAEGALGAARSGEV
                     DALIESLASADPMDAESDYVALFDRGRGTSLHLFEHVHGDSRERGPAMIDLGETYAKA
                     GLILAEGELPDYLPAVLEFASTQPPREARAFLGETAHILNAIFGALQQRRSAYAAALG
                     ALLELAGEAVQPVKPAAEEPLDESWAEPPVFDGCSSKGQARPGQPQPVRLVRAGSKPG
                     ALA"
     gene            complement(1664..>2855)
                     /gene="narH"
                     /locus_tag="VI195_04320"
     CDS             complement(1664..>2855)
                     /gene="narH"
                     /locus_tag="VI195_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEY6823638.1"
                     /translation="QQAGESRTMPTARPRSLISGERMEKIEWGPNWEEILGGEFAGRS
                     KDRNFDDVQKEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQ
                     DKCRGWRMCVSGCPYKKIYYNWKSGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGV
                     LLYDADRIQQAASVEHDRDLYQAQLDIFLDPNDPKVIAQAAVDGIPDKWMEAARSSPV
                     YKMAVQWKVALPLHPEYRTLPMVWYVPPLSPISAAANAGQVGSNGQIPDVNQLRIPVK
                     YLANLLTAGDTAPVVRALERMLAMRAYQRDKHVDRRINTAVLEQVGITQDEVDEMYQV
                     MAIANYEDRFVIPSTHREYAENTFNVRGGCGFSFGNGCSEGTGETSLFGSEKRRTIPI
                     KAEV"
BASE COUNT          449 a          949 c          990 g          467 t
ORIGIN      
        1 cgaagggcat cctccgcggg ctcgggcagc tgcagcgcct gctcgagcag cgcctcgatg
       61 gcgcgggagg ccgcctcgct gatgacacca tcggcgctgg ccgtgtcgct cgcatccagc
      121 aggcccgccg cctgcgcggc ctggcgaagc agctcggtgc acgcgcgctg gcgcaatgcc
      181 tcggctgaaa gggtttcgtc cccggcatgc aggacgacgc cgttgacgga tgccctaggc
      241 acggacgcgg ggctggttct ggccttcggg caggttgagg cgccggctgc gcacgacctg
      301 ctgcgggcgg aacagatagg caatcgtggc gaagccgctc cagacgtgca ccagccggct
      361 gaaggggaac agcaggaaga tggtcatccc cagcacgatg tgcaccttgt acggccaggc
      421 gatcggaatc aacccttccg cgccgccgac cctgaacgtc atcacgcgct gcgcccagtc
      481 cgccagcacc agcatgacgc tgccgtcgga atgcgaaagc gagaacggca gcgtgatcag
      541 gccgagcgtc agctgcaccc agaggatgat caggatcgcc aggtcggtgc gatggctggt
      601 caggcggatg cgggcatcga aaatgcggcg gtggatgagc aggctcagcc ccacgaagca
      661 gaggaatccg gcgacgccgc ccgcgtagac ggcgagctgc tgcttgtggg ctacgctgat
      721 gaacggctcg tagacccagt gcggggtgag cagccccacg aaatggccga tgaacaggaa
      781 caggacgccc gcgtggaaca ggttgctgcc ccagcgcagc gtgccggtgc gcagcatctg
      841 cgatgagtcg ctcttccagg tgtactggtc gcggtcgaag cgcgccaggc tgcccatgag
      901 gaagaccacc atgcagatgt acgggtagga gccgaagaag aagacgtgca gatagttttc
      961 cagggtgctc atgcgagcgc tccaggtttg gaacccgcgc ggaccaggcg gacgggttgc
     1021 ggttgaccgg gacgggcctg gcccttggac gaacagccgt cgaacaccgg cggctcggcc
     1081 cagctctcgt ccagcggctc ctcggccgcc ggcttgacgg gctgcaccgc ttcgccggcg
     1141 agctcgagca gcgcgccgag cgcggcggca taggcgctgc ggcgctgctg cagcgcgccg
     1201 aagatcgcgt tgaggatgtg cgccgtctcg ccgaggaagg cgcgcgcctc gcgcggcggc
     1261 tgcgtcgacg cgaattcgag caccgccggc aggtaatccg gcagctcgcc ctcggcgagg
     1321 atcaggcccg ccttggcata ggtctcgccg aggtcgatca tcgccgggcc gcgctcgcgc
     1381 gaatcgccgt gcacgtgctc gaacaggtgc agcgacgtgc cgcggccccg gtcgaacagc
     1441 gccacgtagt cggactccgc gtccatcgga tcggcgctcg cgagcgactc gatcaaggcg
     1501 tccacctcgc cgctgcgcgc cgcgccgagc gcgccctcgg cgcgcaggat ttcgcgcatt
     1561 tcgggcaggt ggctgcgcat ctgcgcgtcc gggtagccga gcagtgccga aagcacgcgc
     1621 agggtcttct gcatcggtcg gagctggtgc gacatgaaca cggtcagacc tccgccttga
     1681 tcggaatcgt cctgcgcttc tcgctgccga acaggctggt ctcgcccgtg ccctccgagc
     1741 agccgttgcc gaacgagaag ccgcagccgc cgcgcacgtt gaaggtgttc tcggcgtatt
     1801 cgcggtgcgt gctcgggatc acgaagcggt cctcgtagtt ggcgatcgcc atgacctggt
     1861 acatctcgtc cacttcgtcc tgcgtgatgc ccacctgctc cagcactgcc gtgttgatgc
     1921 gccggtcgac gtgcttgtcg cgctggtagg cgcgcatcgc gagcatgcgt tccagcgccc
     1981 gcaccaccgg cgccgtgtcg cccgcggtca gcaggttggc caggtacttc accgggatgc
     2041 gcagctggtt gacgtcgggg atctggccgt tcgagccgac ctgccccgcg ttggccgcgg
     2101 cgctgatcgg cgagagcggc ggcacgtacc agaccatcgg cagcgtgcgg tactcggggt
     2161 gcagcggcag cgcgaccttc cactgcaccg ccatcttgta gaccgggctc gagcgcgcgg
     2221 cctccatcca cttgtccgga atgccgtcga cggcagcctg ggcaatcacc ttggggtcgt
     2281 tcgggtcgag gaaaatatcg agctgcgcct ggtagaggtc gcggtcgtgc tcgacgctcg
     2341 ccgcctgctg gatgcggtcg gcgtcgtaca gcagcacgcc gaggtaacgg atgcgcccca
     2401 cgcaggtttc cgagcacacg gtcggctggc cggcctcgat gcgcgggtag cagaagatgc
     2461 acttctcggc cttgccgctc ttccagttgt agtagatctt cttgtacggg cagccgctca
     2521 cgcacatgcg ccagccgcgg cacttgtcct ggtcgatgag cacgatgccg tcttcctcgc
     2581 gcttgtagat cgagccggag gggcaggagg ccacgcaggc cggattcagg cagtgctcgc
     2641 acagtctggg caggtacatc atgaaggtgt tctcgaactg gccgtagatc tccttctgca
     2701 cgtcgtcgaa gttgcggtcc ttgctgcggc ccgcgaattc gccgccgagg atctcctccc
     2761 agttcggccc ccactcgatc ttctccatgc gctcgcccga gatcagcgag cgcggccggg
     2821 cggtcggcat ggtccggctc tcgccggcct gttgc
//
LOCUS       DATDMP010000254         5168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1262156, whole genome shotgun sequence.
ACCESSION   DATDMP010000254 DATDMP010000000
VERSION     DATDMP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262156"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VI195_04325"
     CDS             <1..412
                     /locus_tag="VI195_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823639.1"
                     /translation="AQALAVARANAAQLALSGIDFRQGSWFEPLAGERFDLVLSNPPY
                     IGSDDPALADPALAHEPRLALTPGADALACLRAIVRGAPQHLAPGGWLLLEHGTAQAA
                     ALRAELVLAGFRSLRSHRDLAGHERVMEGRYDQI"
     gene            399..890
                     /locus_tag="VI195_04330"
     CDS             399..890
                     /locus_tag="VI195_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6823640.1"
                     /translation="MIRFDTSHGAFTVELFPKEAPITVENFLRYVDDGHFDGTIFHRI
                     VPGFVIQGGGLTADFASKKTRAPIGNEAKNGLKNTRGSLSMARTSDINSATSQFFVNL
                     ADNAFLDHSARDYGYAVFGRVSEGMDVIDRIARVGTGTRKGYQDAPLEDVVIVSARRV
                     PPA"
     gene            908..1675
                     /gene="mtgA"
                     /locus_tag="VI195_04335"
     CDS             908..1675
                     /gene="mtgA"
                     /locus_tag="VI195_04335"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY6823641.1"
                     /translation="MTAPVRGLAPATRRGRIAAALLKFLGVALLAWLVLTVTPVLVLR
                     WLRPLTSAFMVEARVAAWQARERGYHTDFRWVSLEQISPSAAMAVIASEDQQFPFHAG
                     FDFNSIREAVRASEHGKRLRGASTISQQVAKNLFLWPGHSFLRKALEAYLTVLIETLW
                     PKERILEMYLNIAQFGAGIYGVQAAAERFWHKPALRLSAAESAALAAALPNPLRLHAE
                     RPSHYLIARRDWILAQMRELGGPAYLRALESEGRLAR"
     gene            1804..2400
                     /locus_tag="VI195_04340"
     CDS             1804..2400
                     /locus_tag="VI195_04340"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY6823642.1"
                     /translation="MADRALGASDWRGFAREQFPQGSWSVDGDVLRAVAQSERVDLVS
                     RECFGDFTLWFDWRLPRAGSTAVLCRVQEAAGPAAHTGPAMQLLDDEHHPDGADALTS
                     CGALYGLMAPWRDLRNSANTYHSARIVMKGSALEYWIDDVQVIGCDLASEELRARIAR
                     SRFRDYPQFARLAEGHIVLQHRGTEAWFRRMHIEVTAG"
     gene            complement(2410..3381)
                     /locus_tag="VI195_04345"
     CDS             complement(2410..3381)
                     /locus_tag="VI195_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY6823643.1"
                     /translation="MAAARANHARFDWQSYLPLAPRVPGVQSFDEYPLAQLLGYIDWM
                     PFFNAWEFTGKFPDILSDPVVGEAASNLYADARRMLKTLIAERWLTARAVLGLFPANA
                     VGDDVQIYASEARREPLVTVNFLRQQKGKPEGQAHECLADYVAPRESGVRDYCGAFAV
                     TAGLGIEEHLERFQRTHDDYSAIMLKALADRLAEAAAEHFHERVRRELWGYAADEALT
                     NEQLVREEYRGIRPAPGYPACPDHTEKAKLWRLLDAERNAGIRLTDSYAMYPTAAVSG
                     WYIAHPQAHYFAIGKIDRDQVEDYARRKGWTLAEAQRWLAPNLGYEP"
     gene            complement(3382..>5168)
                     /locus_tag="VI195_04350"
     CDS             complement(3382..>5168)
                     /locus_tag="VI195_04350"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY6823644.1"
                     /translation="CGTTPEHIAAMRDALSGVTPRRPAARPVALRLAGLEPLNVDDQS
                     LFVNVGERTNVTGSKAFARLILAGDYAGALAVARQQVENGAQVVDINMDEAMLDSQKA
                     MTTFLSLLAVEPDIARVPVMIDSSKWTVIEAGLRCVQGKAVVNSISLKEGEQPFIEHA
                     RLVRRYGAAAVIMAFDERGQADSIERRVAICQRAYRVLTERVGFAPEDIIFDPNIFAI
                     ATGIEEHNDYGRAFIEATRRIKQELPHTLVSGGVSNVSFSFRGNEPVREAMHSVFLYH
                     AIAAGMDMGIVNAGQLGVYEQLAPALREACEDVVLNRRADATERLLEMAAQYKNEGGT
                     RRSEDREWRKLAIGKRLEYALVKGIDDYIIEDTEAARQASARPLDVIEGPLMDGMNVV
                     GDLFGSGKMFQPQVVKSARVMKRAVAHLVPYIEQGKEASAPRSNGRIVVATVKGDVHD
                     IGKNIVGVVLQCNNFEVIDLGVMVPCEKILETARRERADLIGLSGLITPSLDEMVHVA
                     REMQRQGFELPLLIGGATTSPAHTSVKIALQYRSPVVYVKDASRAVGVCQTLTQPTQR
                     AAFIARVSAEHERRREQHAAKKVKVPEA"
BASE COUNT          782 a         1767 c         1729 g          890 t
ORIGIN      
        1 ggcacaggcg ctggcggtcg ctcgcgccaa cgcagcgcaa ctcgcgctca gcggcatcga
       61 cttccggcag ggcagctggt tcgagccgct cgcgggcgag cgcttcgatc tcgtcctcag
      121 caatccgccc tacatcggca gtgacgaccc ggcgctcgct gatcccgcgc tcgcgcacga
      181 gccgcggctg gcgctcacgc cgggagccga tgcgctcgcc tgcctgcgcg ccatcgtacg
      241 cggcgcgccg cagcacctcg ctcccggcgg ctggctgttg ctcgagcacg gcaccgcaca
      301 ggcagcggcg ctgcgcgctg agcttgtcct tgcgggcttt cgttcgctac gctcgcaccg
      361 cgatctcgcc ggccacgaac gcgtaatgga aggacgatat gatcagattt gacacctcgc
      421 acggcgcctt cacggttgag ctgttcccca aggaggcgcc gatcaccgtg gagaacttcc
      481 tgcgctacgt cgatgatggg cacttcgacg gcacgatctt ccaccgtatc gtgccgggtt
      541 tcgtcatcca gggcggcggg ctcacggcgg atttcgccag caagaagacg cgcgcgccca
      601 tcggcaacga ggccaagaac ggcctgaaga acacgcgcgg cagcctgtcg atggcgcgca
      661 ccagcgacat caacagcgcc acctcgcagt tcttcgtcaa cctggccgac aacgccttcc
      721 tcgaccacag tgcgcgcgac tacggctatg ccgtgttcgg gcgcgtcagc gagggtatgg
      781 acgtgatcga ccgcatcgcg cgggtcggta ccggcacgcg caagggatac caggacgctc
      841 cgctcgagga cgtcgtgatc gtctcggcac gacgcgttcc gcccgcctga ggagcaagcc
      901 cggtcacgtg actgcacctg tgcgcggctt agcgcccgcg acccggcgtg gccgcatcgc
      961 cgccgcgttg ctcaagttcc tcggggtggc gctgctcgcc tggctggtgc tcacggtgac
     1021 gccggtgctg gtgctgcgct ggctgcgccc gctcaccagc gctttcatgg tggaggcgcg
     1081 cgttgccgcc tggcaggcac gcgagcgcgg ctatcacacc gatttccgct gggtgagcct
     1141 cgagcagatc tcgccaagcg cagccatggc ggtgatcgct tcggaggatc agcagtttcc
     1201 gtttcacgcc gggttcgatt tcaactcgat ccgcgaggcg gtgcgcgcca gcgagcacgg
     1261 caagcgcctg cgtggcgcca gcacgatctc tcagcaggtc gcgaagaatc tatttctctg
     1321 gccagggcac agcttcctgc gcaaggcctt ggaggcctac ttgaccgtgc tcatcgagac
     1381 gctctggccg aaggaacgca ttctcgagat gtatctgaac attgcgcagt tcggcgctgg
     1441 catttacggc gttcaggccg ccgccgagcg cttctggcac aagccggcgc tgcgcttgag
     1501 cgccgcggag tcggcggcgc ttgcggccgc gctgccaaac ccgctgcgtc tgcacgccga
     1561 gcggccttcg cactacctca tcgcgcgccg cgactggata ctcgcgcaga tgcgcgagct
     1621 cggcggtccg gcctatctgc gcgcgctcga gagtgagggt cgcctcgccc gctgagccgt
     1681 tcccgcgcac gaccggggca caaccgcggg cgtggttcac gaactgcgcc gctcgcccct
     1741 ttcgggccat gtcaccggca attttcctgc ggtaggatcg ctgcatgggc cggagaggga
     1801 gttatggctg atcgtgcctt gggtgcgagt gactggcgcg ggttcgcccg cgagcagttt
     1861 ccgcagggat cgtggagcgt ggacggagat gtgctgcgcg ccgtcgcgca gagcgagcgc
     1921 gtcgatctcg tctcacgtga atgctttggg gatttcacgc tgtggttcga ctggcgcttg
     1981 ccgcgcgcgg gcagcacggc ggtactgtgc cgggtgcagg aggcggctgg gccagcggcc
     2041 cacaccggcc ctgccatgca actgctcgat gacgagcatc accccgacgg ggccgacgcg
     2101 ctcacctcct gcggcgcgct ctacgggctc atggccccgt ggcgcgacct gcgcaacagc
     2161 gccaatacat accacagcgc gcgcatcgtc atgaagggct cggcactcga gtactggatc
     2221 gacgatgttc aggtgatcgg ctgtgacctc gcgagcgagg agctgcgggc gcggatcgcg
     2281 cgcagccgct tccgagacta cccgcagttc gcacgcctcg ccgagggaca catcgtgttg
     2341 cagcaccgcg gcacggaagc ctggttccgc cgcatgcaca tcgaagtgac ggcgggctag
     2401 cgcaagcgcc taaggttcgt aaccgagatt cggcgccagc cagcgctgcg cctccgcgag
     2461 cgtccagccc ttgcggcgcg cgtaatcctc gacctggtcg cgatcgatct tgccgatcgc
     2521 gaagtaatgc gcctgcgggt gcgcgatgta ccagccgctc accgccgcgg tcggatacat
     2581 ggcgtaggaa tcggtgagac ggatgccggc gttgcgctcc gcatcgagca gccgccagag
     2641 cttcgctttc tcggtgtgat cgggacacgc gggataccct ggcgccggac ggatgccgcg
     2701 gtactcctca cgcaccagct gctcgttggt cagggcctca tcggccgcgt aaccccacag
     2761 ctcgcggcgc acccgctcgt ggaagtgctc cgccgcggct tcggcgagac gatcggcgag
     2821 cgccttgagc atgatggcgg agtaatcatc gtgcgtgcgc tggaaacgct ctaggtgctc
     2881 ctcgataccg agtcccgcgg tcaccgcgaa ggctccgcag tagtcgcgca cgccgctctc
     2941 gcgcggcgcc acgtagtcag ccaggcactc gtgcgcctgc ccctccggct tgcccttctg
     3001 ctggcgcagg aagttgacgg tcacgagcgg ctcgcgccgc gcctcgcttg catagatctg
     3061 cacatcgtcg cccacggcgt tggccgggaa cagcccgagc acggcgcgcg ccgtgagcca
     3121 gcgctcggcg atcaacgtct tcagcatgcg gcgcgcatcg gcgtacaggt tgctcgccgc
     3181 ctctcccacc accgggtcgc tgagaatgtc gggaaatttg ccggtgaact cccaggcatt
     3241 gaagaacggc atccagtcga tgtagccgag cagctgcgcc agcgggtatt cgtcgaagct
     3301 ctgtacgccc ggtacgcgcg gcgcgagcgg caggtagctc tgccagtcga agcgtgcatg
     3361 gttggcgcgc gcggccgcca gctaggcttc cggcaccttc accttcttgg cggcatgttg
     3421 ctcgcgccgg cgctcgtgct cggcgctgac gcgggcgatg aacgccgcgc gctgcgtcgg
     3481 ttgcgtgagc gtctggcaga caccgacggc gcgcgaggcg tccttgacgt acaccaccgg
     3541 cgagcggtat tgcaaggcga tcttcaccga agtatgtgcc ggtgaggtgg tggcaccgcc
     3601 gatgagcagc ggcagctcga agccctggcg ctgcatttcg cgcgccacgt gcaccatctc
     3661 atcgagcgag ggggtaatca gtcctgagag gccgatgaga tcggcgcgct cgcggcgcgc
     3721 cgtctcgagg atcttctcgc acggcaccat cacgccgagg tcgatgacct cgaagttgtt
     3781 acattgcagc accaccccga cgatgttctt gccgatgtcg tgcacgtcgc ccttgacggt
     3841 cgcgaccacg atgcggccgt tggagcgcgg cgcggaagct tccttgccct gctcgatgta
     3901 cggcacgagg tgggctaccg cgcgcttcat cacgcgcgcg ctcttcacca cctgcggctg
     3961 gaacatcttg cccgagccga acaggtcccc gaccacgttc atgccgtcca tgagcggccc
     4021 ttcgatgaca tcgagcgggc gggcgcttgc ctgccgggcg gcctcggtat cctcgatgat
     4081 gtagtcgtcg attcccttga ccagggcata ctcgaggcgc ttgccgatgg cgagcttgcg
     4141 ccactcgcga tcctcgctgc ggcgtgtgcc gccctcgttc ttgtattgcg ccgccatctc
     4201 cagcagccgc tcggtggcgt cggcgcggcg attgagcacc acgtcctcac atgcctcacg
     4261 cagcgccggt gcgagctgct cgtacacccc gagctgcccg gcgttgacga tgcccatgtc
     4321 catgccggcg gcgatcgcgt ggtagaggaa caccgaatgc atcgcctcgc gcaccggctc
     4381 gttgccgcgg aaggagaacg acacgttgct cactccgccg ctcaccagcg tgtgcggcag
     4441 ctcttgcttg atgcgccgcg tcgcctcgat gaatgcgcgc ccgtagtcgt tgtgctcctc
     4501 gatgccggtg gcgatggcga aaatgttggg atcgaagatg atgtcctcgg gcgcgaaacc
     4561 gacgcgctcg gtgaggacgc ggtaagcgcg ctggcagatg gcgacccgcc gttcgatgct
     4621 atctgcctga ccgcgctcat cgaaggccat gatcaccgcc gcggcgccgt agcggcgcac
     4681 cagccgcgca tgctcgatga agggctgctc gccctccttg aggctgatgg agttgacgac
     4741 cgccttgcct tgcacgcagc gcaggcccgc ctcgatcacc gtccacttgg aggagtcgat
     4801 catcaccggc acgcgcgcga tgtccggctc gacggcgagc aggctgagga acgtcgtcat
     4861 cgccttctgc gagtcgagca tcgcctcgtc catgttgatg tcgacgacct gcgccccgtt
     4921 ctccacctgc tggcgcgcca cggcgagggc gcccgcgtag tcgccggcga ggatcaggcg
     4981 ggcgaaggcc ttcgaaccgg tcacgttcgt gcgctcgccg acattcacga aaagcgactg
     5041 gtcgtcgaca ttgagtggct cgagcccggc gaggcgcagg gcgaccgggc gggcggcggg
     5101 ccggcgcggt gtgacaccgc tcagtgcatc gcgcatcgcc gcgatgtgct ccggcgtggt
     5161 gccgcagc
//
LOCUS       DATDMP010000255         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1150357, whole genome shotgun sequence.
ACCESSION   DATDMP010000255 DATDMP010000000
VERSION     DATDMP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150357"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VI195_04355"
     CDS             <1..435
                     /locus_tag="VI195_04355"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY6823645.1"
                     /translation="RWPNSRKHRDDDAAAELGRRIWEGSKASSGTGGPLKLSVRGTNF
                     QIKVWRALLELGSAGLTTYAALAQAIGTAGAAQAVGNAVGANPIAWLIPCHNVLRKDG
                     ALGGYRWGSERKRAMLAWSSLRAFQPPLGHATSGRAAAGHVE"
     gene            739..>2123
                     /locus_tag="VI195_04360"
     CDS             739..>2123
                     /locus_tag="VI195_04360"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY6823646.1"
                     /translation="MSASPYKQLEQEFKRLHAFRGALALLRWDAAVMMPRGSADVRGD
                     QLAALETEHHALLTAPRIIRLLDRAQANTQGLADWQIANLREMRRQRDHAIATPVSLV
                     SRLTKAASRAEARWLEARTQGKFEAFAPYLEEVVHLVRDKATLLGQALNLAPYDALVD
                     EFSPGLTTADIDAMFKALSRRLPSLIREAIVTQEGRPLLPLNGKFPSVKQRALVMEVM
                     KAVGFPFDRGRVDESEHPFTEGVPGDIRVTTRFDADDVFTGLLGALHETGHAMYDLGL
                     PHEWRDQPVGRDRGMALEESQSLLIEMMVCRSRPFVRYVQPLLAKHFGTGGPEWEVEN
                     VYGHLTRVQRGLIRVDADELTYPLHIMLRYELEKQLLSGELAVRDLREAWNAGMEQRL
                     GIRPSNDLEGCLQDIHWAVGSFGYFPSYALGAVIAAQLYESLRAELPALDEQLSRGEF
                     SGLFGWLRTHV"
BASE COUNT          356 a          689 c          709 g          369 t
ORIGIN      
        1 cgctggccga actcccgcaa acatcgcgat gatgatgcgg cggctgagct cggccggcgc
       61 atctgggagg ggtcgaaggc gagctccggg acgggagggc cgctcaagct ctcggtccgc
      121 ggcaccaact tccagatcaa ggtctggcgg gcgttgctcg agctgggctc tgcaggtctc
      181 accacctacg cagcgctggc acaggcgatc ggcactgcgg gtgcggcgca ggcggtgggt
      241 aatgcggtcg gcgccaaccc catcgcgtgg ctcatcccct gccacaacgt actgcgcaag
      301 gacggagctc tcggcggcta ccgctggggc tccgagcgca agcgggcgat gctggcctgg
      361 agctcgctgc gcgcctttca gccccctctc ggccatgcca catcgggtcg agccgctgcc
      421 ggtcacgtgg aataaagcga ggaccgagcg gaggccaggg cttcccgctc gcggccagca
      481 gacataacgc ggaagctgcg ctccaggtgc gcgcatgtcg tgagcggctc agcacagaca
      541 gagctgccca caagaggccc ctcgaggtgc gccgcttgcg agcccacttt cgagcggcgc
      601 cgtcaagggc accgttctgt caaatgcgcg tcatattagg taagtggatg atttacatag
      661 ataatcgacg cgttcacgca ggcgctggca cggcagaact ggtagactat gcgcgtcccc
      721 tgatcgtcga cgagatccat gtccgcctcc ccatacaagc aactcgaaca ggagttcaag
      781 cgcctgcatg ccttccgtgg cgccctcgcg ctgctgcgct gggacgcggc ggtgatgatg
      841 ccccgtggga gtgccgacgt ccgtggtgat cagctcgctg cgctcgagac cgagcaccac
      901 gcactgctga ccgccccgcg catcattcgt ctgctcgatc gggctcaggc caatacgcag
      961 ggactggctg actggcagat cgccaacctg cgcgagatgc gccggcagcg cgatcacgcc
     1021 atcgcgacgc ccgtgtcgct ggtctcgcgg ctgaccaagg cggcctcgcg ggccgaggcg
     1081 cgctggctcg aagcccggac acaaggcaag ttcgaggcgt tcgcgcctta cctcgaagaa
     1141 gtcgttcacc tggtacgcga taaagcgaca ctcctcggcc aggcgctcaa cctcgcgcct
     1201 tatgacgccc tggtggacga gttcagtccg ggtctcacca ccgcggacat cgatgctatg
     1261 ttcaaggcgc tctcgcgccg tcttccctcg ctcatccgcg aggcgatcgt gacccaggag
     1321 ggccggccgc tgctgccgct caacggtaaa ttcccttcag tgaagcagcg ggcgctcgta
     1381 atggaggtca tgaaggccgt cggattcccc ttcgatcgcg gacgggtgga tgagagtgag
     1441 caccctttca ccgagggggt tcccggggac atccgggtca cgacgcgttt cgatgcggat
     1501 gatgtgttca ccggactcct cggtgcgctg cacgagaccg gtcacgccat gtatgacctg
     1561 gggctccccc acgagtggcg cgaccagccg gtgggacgcg accggggtat ggcgctcgag
     1621 gagagccagt cgctgctcat cgagatgatg gtctgccgca gccgcccatt cgtgcgctac
     1681 gtgcagccgc tgctcgccaa gcacttcggc acgggcggac cggaatggga ggtcgagaac
     1741 gtctatggtc atctgacgcg ggttcagcgg ggactgatcc gtgtggatgc ggacgagctc
     1801 acctatccgc tgcacataat gctgcgttat gagcttgaaa agcagctgct gtccggggag
     1861 ctggcggtgc gcgatctccg cgaggcgtgg aacgccggca tggaacagcg gctggggatt
     1921 cgtccctcga acgatctcga gggctgcctg caggacatcc actgggcggt cggctccttt
     1981 ggctatttcc cctcctatgc gctcggagcc gtcattgcgg cccagctcta cgagagcctg
     2041 cgcgcggagc ttcccgcgct cgatgagcag ctctcgcgcg gggagttctc gggcctcttc
     2101 gggtggctac ggactcacgt gca
//
LOCUS       DATDMP010000256         2953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1483735, whole genome shotgun sequence.
ACCESSION   DATDMP010000256 DATDMP010000000
VERSION     DATDMP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483735"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VI195_04365"
     CDS             <1..685
                     /locus_tag="VI195_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY6823647.1"
                     /translation="AGGGEMATTRCGLGSFGQAKALSTRNDAPTEASRPWDKDRDGFV
                     LSDGGGAVLLEELEHARARGARIYAELVGFGMSGDAHHITAPPEDGEGARIAMVNALR
                     DAQLNAGEVQYVNAHATSTPLGDAAETVALKRAFGEHAYRMAVSSTKSMTGHLLGAAG
                     VVEAIFSVLAIRDNVAPPTINYRTPDPECDLDYVPNSARQMKIDVSLSNSFGFGGTNG
                     SLIFRRSDS"
     gene            751..2109
                     /locus_tag="VI195_04370"
     CDS             751..2109
                     /locus_tag="VI195_04370"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF006563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY6823648.1"
                     /translation="MLRRLAAHRPQRYPLLLDSAAQGPLGRTSLLLAAPSAALWLDRG
                     GTLGAQGMTPRGAGFLTALENWWRSECVPHAGGEGADALIAGGWAVFLGYELAAEIEP
                     NLALPAAPLPWQAFALRTHGAIELDLQTGRICAVAEDAAILGRLVADALEAARVPAEE
                     ESLRIESVREEDPAAYLARVLRAKEYVGAGDIYQANLSRPWDVLMRIPERNWQAAASA
                     RPVIAATLYQRLCAANPAPFAALAQWQGISILSSSPERLVRVAGRRVDTRPIAGTRPR
                     SRDPAREAREMSELAAHPKERAEHIMLIDLERNDLGRVCEAGSVRVDELMTIESYQHV
                     HHIVSNVSGVLRPEVTPIGAVRAVFPGGTITGCPKFRCMQIIAELEGAGRGAYTGSLG
                     YLTRDGRLDLNILIRTMTLRGHALSFRAGAGIVADSDPERELEETRAKARGLLAALEE
                     PR"
     gene            2109..>2953
                     /gene="pabC"
                     /locus_tag="VI195_04375"
     CDS             2109..>2953
                     /gene="pabC"
                     /locus_tag="VI195_04375"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEY6823649.1"
                     /translation="MGDLERVLIDGQRFTASASVPVLERALHYGDALFETIACLEGRP
                     RLLERHLRRLAMGCERLGLGAPVAPLASELAELAHGTSRAIIKLLVTRGVALARGYAL
                     TGAERTRRIALRYAWPPEDPAGQDEGIRVRLATLRLGENPALAGIKHCNRLEQVLARR
                     EWTDPGIAEALLFSSSGALVSGTMSNVFLVRGSKLLTPAIDRCGVAGVMRGLVLELAA
                     AAGIAAQERRLDAADLEAAEELFLTNALGGIRPVRELAGVRRARGPLTRLLQERLAAH
                     LAAEV"
BASE COUNT          432 a         1026 c         1043 g          452 t
ORIGIN      
        1 cgccggtggc ggcgagatgg cgaccactcg ctgcgggctc ggcagcttcg gacaggccaa
       61 agcgctctcc acgcgcaatg acgcacccac ggaagccagc cggccgtggg acaaggatcg
      121 cgacggtttc gtgctgagcg atggcggcgg cgcggtgctg ctggaggagc tcgagcacgc
      181 acgtgcgcgc ggcgcgcgca tctatgcgga gctggtcggc tttggcatga gtggggatgc
      241 gcaccacatc accgccccgc ccgaggacgg cgagggcgca cgcatcgcca tggtgaatgc
      301 gctgcgcgat gcccagctca atgcgggcga ggtgcagtac gtcaatgcac acgccacctc
      361 gacccccctc ggtgatgcgg cggagaccgt agcgctcaaa cgcgccttcg gcgagcacgc
      421 ttaccgcatg gcggtcagct ccaccaaatc catgaccgga cacctgctcg gcgctgccgg
      481 ggtggtcgag gcaatcttct cggtgctggc gatccgtgac aacgtcgcgc cgccaaccat
      541 caactaccgc acgcccgatc ccgagtgcga cctggactac gtgccgaaca gcgcgcggca
      601 gatgaagatc gacgtaagcc tctcgaactc gttcggtttc ggcggcacta acggctcact
      661 catcttccgg cgttcagact cctgagcgca ctgccccggg accctgccac gcgcgctccc
      721 ttcgttcgcg atctcgagct tccgccacag gtgctgcggc gcctggcggc gcatcgcccg
      781 cagcgctatc cgctgctgct cgacagcgca gcgcaggggc cgctcggccg cacgagtctg
      841 ctgcttgcgg cgccctccgc ggcgctgtgg ctcgatcgtg gcggcacgct gggcgcgcag
      901 ggcatgacac cgcggggggc gggattcctc accgcgctcg agaactggtg gcgcagcgag
      961 tgtgtcccgc acgcgggcgg cgaaggcgcc gatgccctca tcgcgggcgg ctgggcggtg
     1021 ttcctcggct acgagcttgc ggcagagatc gagccgaatc tcgcgctacc ggcggcgccg
     1081 ctcccctggc aggcgttcgc attgcgcact cacggtgcga tcgagctcga tctgcaaacc
     1141 gggcgcatct gcgcggtggc ggaggacgcc gcgattctcg gccgcctggt tgccgatgcg
     1201 ctcgaggcgg cgcgcgtgcc ggcggaggag gagtccctgc gcatcgagag cgtgcgtgaa
     1261 gaggaccccg cggcctatct cgcgcgcgtg ctgcgtgcca aggaatacgt cggcgcgggc
     1321 gacatctacc aggcaaatct ttcccgcccc tgggacgtgc tcatgcgcat ccccgaacgg
     1381 aactggcagg cagcggcgag cgcgcgcccg gttatcgcgg ctacgctcta tcagcgattg
     1441 tgtgcggcga atccggctcc ctttgcggca ctcgcgcagt ggcaggggat ctcgattctc
     1501 agctcctcgc ccgagcggct ggtgcgcgtc gccggacggc gtgtcgacac gcgcccgatc
     1561 gccggcacac gtccgcgcag ccgcgacccc gcgcgcgagg cccgcgagat gagtgagctc
     1621 gccgcccatc ccaaggagcg cgccgagcac atcatgctga tcgacctgga acgcaacgac
     1681 ctcggccggg tttgcgaggc gggcagcgta cgggtcgatg agctcatgac catcgagtcc
     1741 taccagcacg tgcaccacat cgtctcgaac gtgagcggcg tgctgcggcc cgaggtcacg
     1801 ccgatcggag cggtgcgcgc ggtgtttccg ggcggcacca tcaccgggtg tcccaagttc
     1861 cgttgcatgc agatcattgc cgagctcgag ggcgcggggc gcggtgcgta caccggctca
     1921 ctcgggtacc tgacacgcga tggccgcctc gacctcaaca tcctcatccg caccatgacg
     1981 ctgcgcgggc acgcgctctc ttttcgggcg ggggcgggaa tcgtcgcgga ctccgatccg
     2041 gagcgggagc tcgaggagac gcgcgccaag gcgcgcgggc tgctcgccgc actcgaggag
     2101 ccgcgctgat gggcgatctc gagcgggtcc tcatcgatgg gcagcgtttc actgcctccg
     2161 ccagcgtgcc ggtgctcgag cgcgcgctgc actacggcga tgcgctgttc gagaccattg
     2221 cgtgcctcga ggggcgaccg cgcctgctcg agcgccacct gcggcgcttg gccatgggat
     2281 gcgagcggct cggcctcggc gctccggttg cgccgctcgc gagcgagctg gccgagctgg
     2341 cgcacggcac ttcgcgtgcg atcatcaagc tgctcgtcac acgcggtgtg gcactcgcgc
     2401 gcggctatgc gctcacaggc gccgagcgca cgagacgcat cgccctgcgc tatgcatggc
     2461 cgcccgagga tcccgcgggc caggacgagg gcatacgcgt gcgcctggcc acgctgcggc
     2521 tcggtgagaa tcccgcgctc gccggcatca agcactgcaa ccgcctggaa caggtgctgg
     2581 cgcgccgcga gtggactgac cctggaatcg ccgaggcgct gctgttcagc agctcgggtg
     2641 cattggtttc aggcaccatg agcaacgtgt tcctggtacg cggatcgaag ctgctgacgc
     2701 ccgcgatcga tcgctgcggc gtcgccggag tcatgcgcgg gctggtactg gagctcgcgg
     2761 cggctgccgg gattgcggcg caggagcgcc ggctcgatgc ggcggatctc gaggcggcag
     2821 aggagctctt cctcacgaac gcgctcggcg gtatccggcc ggtgcgcgag ctcgccggcg
     2881 tccgccgcgc gcgcggtcca ctgacgcgac tcttgcagga gcggctcgct gcgcacctcg
     2941 ccgccgaagt gca
//
LOCUS       DATDMP010000257         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_151322, whole genome shotgun sequence.
ACCESSION   DATDMP010000257 DATDMP010000000
VERSION     DATDMP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151322"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VI195_04380"
     CDS             complement(<1..1196)
                     /locus_tag="VI195_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY6823650.1"
                     /translation="MRYAPLGLLALIICLPCLAGAQSFAPDTPEAGSVEEIAKATTEA
                     RFLSPWVAYLPASSAGAVSPRSFLHRIPGAPGDLVNSATAYAYCRALAGSSPRVRAYT
                     IGRSEEGRDIVLLAIADEAGIRDLERLKAATGALADPRRTDAAAAEQLIATGRPIYYF
                     NAGLHSDESGSTESVLELAYRLAVSEQPMIRRIRENLVVLINPVSNPDGRDKQVEWFY
                     RYLKGKTEQATLPRQAPPYWSHYAFVDINRDAHQQVHETTKAVYRMLFDWHPQVIHDL
                     HESVALLLTWNGTGPINQHVDPITYAERLELSFHEVQTLTGFGMPGVWTWNFGDDWAH
                     LYLDAIGINHNADGRGYETFGNGSAETMRSEQPGDDNTVEWYRPLPPPATNSFLWSAR
                     DNVNYN"
     gene            1262..>2788
                     /locus_tag="VI195_04385"
     CDS             1262..>2788
                     /locus_tag="VI195_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6823651.1"
                     /translation="MARNPRYDVLFEPVRIGPVTAHNRFYAVPHAAGMTNAMPHMRAA
                     FRGTKAEGGWGVVCTGYVSIEPGSDDSPLPYATLWDDDDVRSHALMTEAVHRHGALAG
                     IELWHGGGSVMNRTSRLPTMSPSGTSWMATHVNFMGHQRPRVMEARDIRLLLDCQATA
                     ARRARQAGFDIVYVYAGMGYLPYEFLLPEWNRRTDGYGGSVANRVRIVRELLEVTHEA
                     VGGACAVALRISLEELRARPSEHAPSEAHEVVALLAEVPDLWDVKLDSSPTDCPPSRF
                     APEGSHEPVIDFVKKITRKPVVGVGRFTSPDTMVGQIKRGVLDLIGAARPSIADPFLP
                     VKIDSGREQDIRECIGCNICISSWHDGVPVRCTQNPTAGEEWRRGWHPERIAPAVSAS
                     GVLIVGGGPAGLECALSLGRRGYEVTVAEATDEIGGRLRFETRLPGLSAWGRVLDWRR
                     GQLERLTNVSIYRGNRLSAAEILELQPAHVAIATGAHWARLLYSPLEIPVGELSGTGV
                     F"
BASE COUNT          435 a          909 c          983 g          461 t
ORIGIN      
        1 tcgttgtagt tgacgttgtc gcgcgccgac cagaggaagg agttggtggc agggggcggg
       61 agtggccgat accactccac ggtgttgtca tccccaggct gctcggagcg catggtctcg
      121 gcgctgccgt tgccgaaggt ctcgtagccc cggccatcgg cgttgtgatt gataccgatc
      181 gcatcgaggt acaggtgcgc ccagtcgtca ccgaaattcc aagtccacac ccccggcatg
      241 ccgaagccgg tcagggtctg gacctcgtgg aaggatagct cgagccgctc ggcgtaagtg
      301 atcgggtcga cgtgctggtt gatgggaccg gtgccattcc aggtgagcag cagagcaacg
      361 ctctcgtgca ggtcgtggat cacctgcggg tgccagtcga agagcatgcg ataaaccgct
      421 ttggtggtct cgtgcacctg ctgatgcgcg tcgcggttga tgtcgacgaa cgcatagtgc
      481 gaccagtagg gcggcgcctg ccgtgggagc gtcgcctgct cggtcttgcc cttgaggtag
      541 cggtagaacc actcgacctg cttgtcgcgt ccgtcggggt tggagaccgg attgatgagc
      601 accacgagat tttcgcggat gcgccggatc atcggctgct cggacacggc gaggcggtag
      661 gcgagctcga gcaccgattc ggtcgagccg ctctcatcgg aatgaagtcc ggcgttgaag
      721 tagtaaatgg ggcgcccggt ggcgatgagc tgctcggcgg cggccgcatc ggtgcgccgc
      781 ggatcggcga gcgctcctgt cgcggccttg aggcgctcga gatcgcggat gcccgcctca
      841 tcggcgatcg cgagcagcac gatgtcgcgg ccttcttccg agcgcccgat ggtgtaggcg
      901 cgcacgcggg gcgaggagcc cgcgagcgcg cggcagtagg cgtacgccgt ggcggaattc
      961 accagatccc cgggcgcgcc gggaatgcgg tgcagaaagc tgcgcggcga tactgcccca
     1021 gcgctcgagg ccggcagata cgccacccac ggtgagagga agcgcgcctc ggttgtcgcc
     1081 ttcgcgatct cctccaccga ccccgcctcg ggtgtgtccg gcgcgaagga ctgtgcaccg
     1141 gcaaggcacg gcaggcagat gatgagggcg agcaggccga gtggtgcgta gcgcatgagt
     1201 ggtatcgtac gctattgcct acgctattga gcattcgctc aactagcggc agactcgcgc
     1261 catggctcgc aatccgcgat acgacgtgct gttcgaaccg gtgcgcatcg gcccggtcac
     1321 cgcgcacaac cgcttttacg ccgtgccgca tgccgccggc atgaccaacg ccatgccgca
     1381 catgcgcgca gcgttccgcg gcacgaaagc cgaaggcggc tggggggtgg tgtgcacggg
     1441 ctatgtctcg atcgagcctg gatcggacga ctcaccgctg ccctacgcca cgctctggga
     1501 cgatgacgat gtgcgctccc acgcgctcat gaccgaggcg gtccaccgcc acggtgcgct
     1561 cgccggtatc gagctgtggc acggtggcgg ctcggtgatg aaccgcacca gccgcctgcc
     1621 gacgatgtcg ccctccggta cctcctggat ggcgacccac gtgaatttca tgggtcacca
     1681 gcggccgcgg gtcatggagg cgcgcgacat ccggctgctg ctggattgcc aggcgaccgc
     1741 cgcgcggcgc gcgcgccagg ccggcttcga catcgtgtac gtctatgccg gcatgggcta
     1801 cctgccctac gagttcctgc tgcccgagtg gaatcggcgc accgacggct acggcggcag
     1861 tgtcgcgaac cgtgtgcgca tcgtgcgcga gctcctcgag gtgacacacg aagcggtcgg
     1921 cggtgcctgc gcggtggcgc tgcgcatcag cctggaggag ctgcgtgcgc ggccgagcga
     1981 gcatgcgccc tcagaggcgc acgaggtggt ggcactcctc gccgaggttc cggacctgtg
     2041 ggatgtgaag ctggactcct cgccgaccga ctgcccgccc tcgcgctttg cccccgaggg
     2101 aagccacgag ccggtgatcg acttcgtgaa gaaaatcacc cgcaagccgg tggtgggcgt
     2161 cgggcgcttc acctccccgg acaccatggt cgggcaaatc aagcgcggcg tgctcgatct
     2221 gatcggtgcc gcgcgcccct cgatcgccga ccctttcctg ccggtgaaga tcgacagcgg
     2281 ccgcgagcag gacatccgcg agtgcatcgg ctgcaacatc tgcatctcga gctggcacga
     2341 cggcgtgccc gtgcgctgca cgcagaatcc gaccgccggg gaagaatggc ggcgcggctg
     2401 gcatcccgag cgcatcgcgc ccgctgtgag cgcctccggc gtgctcatcg tcggtggcgg
     2461 cccggcgggg ctcgagtgcg cgctgtcact cggccggcgc ggctacgaag taaccgtggc
     2521 ggaggccacc gatgagatcg gcggtcggct gcgcttcgag acccgcctgc cgggactctc
     2581 ggcctgggga cgggtgctcg actggcgccg cgggcagctc gagcggctca cgaatgtaag
     2641 catctatcgc ggcaaccgcc tgagcgccgc cgaaatcctg gagctgcagc ccgcacacgt
     2701 cgccatcgcg accggtgcgc actgggcgcg cctcttgtac tcgccgctcg agatccccgt
     2761 gggcgagctc tcaggcaccg gggtcttc
//
LOCUS       DATDMP010000258         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1595479, whole genome shotgun sequence.
ACCESSION   DATDMP010000258 DATDMP010000000
VERSION     DATDMP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595479"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VI195_04390"
     CDS             <1..952
                     /locus_tag="VI195_04390"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEY6823652.1"
                     /translation="FEPLFEGRREDALIAYTFEQYLDTGDPDWPLLLPMVKSAVRAMD
                     TVEQLGRERWGLAIERFTVTGASKRGWTSWLTAAVDPRVASVAPMVIDMLNFRAQIPL
                     QRQAFGALSEQIQDYEQIHLPERIDSPTGRELVSMVDPYSYRDKLTQPKLILLGTNDP
                     YWPLKALNAYWSGLPEEKRVLYLPNETHNLRDVDRIIGSIAALHRYSQRGTPLPSLTE
                     VLTEQTDRLEMRVRSDRTPERVLAWSASSRTRDFRQAHWSARDCRRSGDDYLCEARTS
                     ANAYTALYAEALFQDRHEPRFSLSTTVHISGQGEVDQGSE"
     gene            complement(922..1716)
                     /locus_tag="VI195_04395"
     CDS             complement(922..1716)
                     /locus_tag="VI195_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6823653.1"
                     /translation="MAGTIQIGEVSVCLEDQGQGDPLLLLHGFPATRQLWSQVAPLLI
                     EAGYRVLIPDLVGYGESTAPAGVRIDMASQAAWMLALLDRLTVTRPVIIAHDVGSAAA
                     QLMVASAPQRIRGLAVLDGVHAAEWAMDAIASIQTWQPADAHRLFPVLARRLAKSPAL
                     RDMLTAYQGDKGGAQLIRAARDLDPRQTAEIGERLRSSAVPAIVLWGERDEFLSRAEV
                     GEPLAKLLNAPLIELPGGHFTPLDCPMEVAQALRPFLHSLPWSTSP"
     gene            complement(1920..2360)
                     /locus_tag="VI195_04400"
     CDS             complement(1920..2360)
                     /locus_tag="VI195_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823654.1"
                     /translation="MKPTTVHAALIAAAAALGSIQVAHAHECTVSNAAGAYGYTSSGS
                     IVNPAVGPFTSVGHTTLTESGTFSGAQTTSIAGNVIEETIQGAFTVNPDCTGSATVQV
                     YHGSTLARTSVINLVWDNHQHEFRAIFLTSGTNISIDGRKMTDD"
     gene            complement(2576..>3174)
                     /locus_tag="VI195_04405"
     CDS             complement(2576..>3174)
                     /locus_tag="VI195_04405"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY6823655.1"
                     /translation="LWAALVDSNGMGLAVYVPGQYARTGAMAFPGDGTGPADNATAYM
                     NGLTSLTFTPGGVIEGNMYLVPGQATSARAAIYALHRGLPATDIFTPIATTDVPAPGT
                     VISGVSASVAGWAFDNVGVDSVLVYVDGALRGTATLGGSRPDVAAAYPHIAPNDSGWN
                     YALDTTALSNGPHSLVVHARDRTGNEALLAPVAVTVSN"
BASE COUNT          579 a          976 c         1054 g          565 t
ORIGIN      
        1 cttcgagccg ctgttcgaag ggcggcggga ggatgcgctc atcgcgtaca cgttcgagca
       61 gtatctcgac acgggcgatc ccgattggcc cttgttgctc ccgatggtga agagcgcggt
      121 gcgtgccatg gacaccgtgg agcagctcgg ccgcgaacgt tggggactcg cgatcgagcg
      181 ttttaccgtc accggcgcat ccaagcgcgg ctggacctcg tggctcaccg ccgcggtgga
      241 tccacgcgtg gccagcgtcg cgccgatggt catcgacatg ctcaatttcc gcgcgcagat
      301 tccgctgcag cggcaggcgt tcggcgcact gtcggagcag attcaggact atgagcagat
      361 tcatcttccc gaacgcatcg attcgccaac cggacgcgag ctggtgtcga tggtcgaccc
      421 gtacagttat cgcgataagc tgacccaacc gaagctgata ctgctcggca ccaacgatcc
      481 gtactggccg ctcaaggcat tgaatgcgta ctggagcggg ctgccggaag agaaacgtgt
      541 gctctatctg ccgaacgaga ctcacaacct gcgcgatgtc gatcgcatca tcggcagcat
      601 cgccgccttg catcgctatt cgcagcgggg caccccgctg ccgtctctga cggaggtgct
      661 tacggagcag accgaccggc tcgagatgcg tgtgcgcagc gatcgcacac ccgaacgtgt
      721 gctggcatgg agcgcgagca gtcgcacgcg cgattttcgt caggcgcact ggagcgcccg
      781 cgactgcaga cgctcgggcg atgactacct gtgcgaagcg cgcacctcgg ccaatgcgta
      841 tacggctctc tacgccgaag cgctgttcca ggatcggcac gagccgcgtt tctcactctc
      901 gacgacggtc cacatcagcg gtcagggaga ggtggaccag ggcagtgagt gaaggaaggg
      961 gcgtagcgcc tgcgccactt ccatcgggca atcgagcgga gtgaaatgcc cgccgggaag
     1021 ctcgatcagc ggtgcattca gcaacttcgc gagcggctcg ccgacctcgg cgcgggaaag
     1081 gaactcatcc cgttcacccc agagcacgat cgcgggcaca gcgctcgagc gcagccgctc
     1141 gccgatctct gcggtctgcc gcggatcgag gtcgcgtgcg gcgcggatga gctgcgcgcc
     1201 tcccttgtca ccctgatagg cggtcagcat gtcgcggagc gcggggcttt tcgccaggcg
     1261 ccgagcgagc acaggaaaaa gccggtgtgc atccgccggt tgccaggtct gaatgctggc
     1321 gatcgcatcc atcgcccatt cggctgcatg gacaccatcg agcacggcga gcccgcgtat
     1381 gcgctgcggt gcacttgcga ccatgagctg tgcggccgcg gatcccacat cgtgcgcgat
     1441 gatgaccggg cgcgtcacgg tgaggcgatc gagaagcgcc aacatccagg ccgcctggct
     1501 tgccatgtcg atgcgcacgc cggcgggagc cgtcgactcg ccataaccca cgaggtccgg
     1561 aatgagtacg cgatagccgg cttcaatcaa gagtggcgcg acctgcgacc agagttgccg
     1621 cgtcgctgga aaaccgtgca acagcagcag cgggtcgccc tggccctggt cttcgaggca
     1681 gacgctgacc tcaccgattt gaatggttcc agccaaaggc aatccgtacg agagcctagc
     1741 ttgccatcgg cggagtatat cgactggagc gcgttcccag tttgccggcc gaagcgaatc
     1801 agcagccagg cgctcgggca ttccaagccg gacgccgtcc gcccgccgca ggcaattgag
     1861 catccctgcc atccgagaac cgcggcggac ggacggcgtc ggcggtcagt ctcatccgct
     1921 cagtcatcgg tcatctttcg gccgtcgatg ctgatgttgg ttcccgaagt caggaagatg
     1981 gcgcgaaact cgtgctggtg gttgtcccag acgagattga tcacgctggt tcgagcgagc
     2041 gttgagccgt gatagacctg tacggttgcc gaacccgtgc aatcgggatt gaccgtaaaa
     2101 gcgccctgga tcgtttcctc gatgacattc cccgcgatgc tcgtggtctg agcaccgctg
     2161 aacgttccgg attcggtgag agtcgtatgg cccacggagg tgaacggtcc cacggctggg
     2221 ttgacgatcg acccgctgga ggtgtagccg taggctccgg cggcgttgga tacagtgcac
     2281 tcatgcgcgt gcgcaacctg tatggacccg agagcggccg ccgcagcgat cagtgcggcg
     2341 tgcacagtgg ttggtttcat ggaattcccc ttggctgacc ctgtggctgt gaaatgacgt
     2401 gcgcgttgac tccgacatat gaagagccac gctcacccgc attcgggtac ggttggcgtc
     2461 aagacgcgtg cagggaaacg ctcgggcgca caccgggctc agaagtgcgc gcagatgcgg
     2521 ggagtcagga ggtcaaacgc cgggtggccg gcaaggttgt aagtctgcgg tcgtgtcagt
     2581 tgctgactgt aactgccacc ggggcgagca gggcttcatt gcctgtgcgg tctcgtgcgt
     2641 gcacgacgag cgagtgtggg ccgttcgaga gcgctgtcgt gtcgagggcg tagttccagc
     2701 cgctgtcgtt tggtgcgatg tgcggatagg cggctgcgac gtccggccgg gaaccgccga
     2761 gggtcgcggt tccgcgaagc gctccgtcca cgtagaccag gacggaatcg acgccgacgt
     2821 tgtcgaacgc ccaacccgct accgacgcac tcaccccgga aattacggta cccggggccg
     2881 gcacatctgt ggtggcgatg ggtgtgaaga tgtcggtcgc cggcaagccg cggtggagcg
     2941 cgtagatcgc cgcgcgagcc gaggtcgctt ggcccggaac gaggtacata ttaccttcga
     3001 tgacaccgcc gggagtgaac gtcaaactcg tcagcccgtt catgtacgcc gtggcattgt
     3061 ctgcaggtcc cgttccgtcg ccgggaaagg ccatggcgcc ggtgcgcgca tattggcccg
     3121 gtacatacac tgcaaggccc attccgttgg aatcaaccag cgcagcccac aatt
//
LOCUS       DATDMP010000259         2727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_707207, whole genome shotgun sequence.
ACCESSION   DATDMP010000259 DATDMP010000000
VERSION     DATDMP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707207"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VI195_04410"
     CDS             complement(<1..1096)
                     /locus_tag="VI195_04410"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6823656.1"
                     /translation="MSLLPYGLVELPFWGYFLVTFVTIETMFLGITLYLHRDQSHGGL
                     ILHPALRHLFRFWLWFSSGAVTKEWVAVHRCHHAHADRPGDPHSPVVFGLRTVVFEGA
                     ELYRAAARNPQLLRTYGRGTPDDWLEHNLYSRYAYGGIAVFVLAHLILFGIPGIIMIA
                     VHLAAQPFFAGGVINGLGHALGYRSFEMPSRATNLVPWAVLLGGEELHNNHHAFPRSA
                     CFAVQRWEFDIGWWWIRLFRSLGLARVRWLAPRPHLERRRRELDAETVSALFINRMHV
                     LRDYGRRVVLPVCRELARREGQGALPPITPTLLIRHPAVLADEPRRKLRELLEKYEVL
                     RRVVEYRDSLQQLWNENPVRALAQLREWSRR"
     gene            complement(1196..2188)
                     /locus_tag="VI195_04415"
     CDS             complement(1196..2188)
                     /locus_tag="VI195_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY6823657.1"
                     /translation="MIPQSMTAVEIREPGPPQVLRAVQRPVPQAAPGEVLIQVAAAGV
                     NRPDVLQRKGLYPPPPGVTDIPGLEVAGEVVRLGEGVNEPALGAKVCALVAGGGYAQY
                     VSAPAVQCLPVPPTLTLEQAAVLPETVFTVWLNVFERSRLQRGETLLVHGGSSGIGTT
                     AILLAKAFDARVIVTAGSAQKCAACRELGADVAINYHEGDFVEATLRATDGKGADVIL
                     DMVGGDYVPRNVAAAAVEGRIAIIATQGGNKAELDLRALMVKRLTLSASTLRAQPVAN
                     KGRIAAAIKEKVWPLFASRGLQPLIHARFPLADAAGAHRLMESNAHIGKIVLLS"
     gene            complement(2382..>2727)
                     /locus_tag="VI195_04420"
     CDS             complement(2382..>2727)
                     /locus_tag="VI195_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823658.1"
                     /translation="RWSVKGVAVLAAVTAVVALLSLAVMLLWNALVPELFHGPPLQYR
                     EAFGLLLLSRILFGGLRGRGWHGHWRQRMWRERWENMTPEERARLREHFQRRCGHRDL
                     PPGDPAAPPQSN"
BASE COUNT          463 a          946 c          912 g          406 t
ORIGIN      
        1 cccggcggct ccactcgcgc agctgcgcga gtgcccggac tgggttctcg ttccacagct
       61 gctgcaggct gtcgcgatac tcgaccacgc ggcgcagcac ctcgtacttc tcaagcagct
      121 cgcgcagctt gcgccgcggc tcatccgcca gcactgccgg atgacggatg agcaacgtcg
      181 gggtgatcgg cggcagcgcc ccctgcccct cgcgccgcgc cagctcacgg cataccggca
      241 gcacgacgcg ccggccgtaa tcgcgcagca cgtgcatgcg gttgatgaag agcgcgctca
      301 ccgtctccgc atcgagctcg cgccggcgcc gctcgaggtg cggccgcggc gcgagccagc
      361 gtacgcgtgc cagtccgagc gaccggaaca ggcgaatcca ccaccagccg atgtcgaact
      421 cccagcgctg caccgcgaag catgccgagc gcggaaacgc gtgatgattg ttgtgcagct
      481 cctcgccgcc gagcagcacc gcccagggga ccaggttagt ggcccgcgag ggcatctcga
      541 agctgcgata accgagcgcg tgaccgagcc cgttgatgac accgccggcg aagaatggct
      601 gcgccgcgag gtgcaccgca atcatgatga tgccgggaat gccgaagagg atcagatgcg
      661 cgagcacgaa caccgcgatg ccgccgtacg catatcggct gtagagattg tgttcgagcc
      721 agtcatcggg tgtcccgcgc ccataggtgc gcagcaactg cggattacgc gccgcggcac
      781 ggtagagctc ggcaccctcg aacaccacgg tacgcagtcc gaacaccacg ggactgtgcg
      841 gatcgcccgg ccgatcggcg tgggcatggt gacagcggtg taccgccacc cactccttgg
      901 tcaccgctcc cgagctgaac caaagccaga agcggaacag gtgccgcagc gccggatgca
      961 ggatcagacc gccgtgcgac tggtcgcggt gcaggtacag cgtgataccg agaaacatgg
     1021 tctcgatcgt gacaaacgtc acgaggaagt agccccagaa gggaagttcc acgagaccgt
     1081 aaggcaggag actcatgggt atcgcaacta ccgccccgtg cgggcctttc gccacaagcc
     1141 tacgggaatt ctccctggcc ggtgcatctt tctgcctgcg caggtcgcgg cgggctcagc
     1201 tgagcaggac gatcttgccg atgtgcgcat tcgattccat cagccggtgg gcaccggccg
     1261 catccgcgag cgggaagcgc gcgtggatca gcggctgcaa tccgcggctc gcaaacagcg
     1321 gccagacctt ttctttgatc gccgcggcga tgcggccctt gttggcgacc ggttgcgcgc
     1381 gcagcgtcga ggcgctgagc gtcaagcgct tgaccatgag ggcacgcaga tcgagctccg
     1441 ccttgttacc accctgggtc gcaatgatgg cgatgcgtcc ctccaccgcc gcggcagcca
     1501 cgttgcgcgg aacgtagtcg ccgccaacca tgtcgagaat gacgtcggcg cccttgccgt
     1561 ctgtggcacg cagcgtggct tcgacgaaat ctccttcgtg gtagttgatc gcgacgtcgg
     1621 cgccgagctc gcggcaggct gcgcacttct gcgcgctgcc ggcggtgacg atgacgcggg
     1681 catcgaaggc tttggcgagc aggatcgccg tggtgccgat gccgctcgag ccaccgtgca
     1741 cgagcagcgt ctcgccccgc tggagccgcg agcgctcgaa gacgttcagc cagaccgtga
     1801 atacggtttc cggcagcacg gctgcctgct cgagggtcag cgtcggcggc acaggcagac
     1861 actgcaccgc gggggcgctc acgtactggg cgtagccgcc gcctgcgacg agcgcgcaca
     1921 ccttggcgcc gagtgcgggc tcgttcacac cctctccgag acgcacgacc tcgcccgcga
     1981 cctcgagtcc cggtatgtcg gtgaccccgg gtggcggcgg atataagccc ttacgttgca
     2041 gcacatcggg acgattgacg cccgcggccg ccacctgaat cagcacctcc ccgggcgctg
     2101 cttgcggcac cggacgttgc acggcgcgca gcacttgcgg cggtcccggc tcgcggattt
     2161 ccaccgcggt catgctctgg ggaatcatgg gcggtggagc gtgccgcctg gcggcggcgg
     2221 gcgtcaaccc tgtgggggca ggacgggcag cagtcgcccg gtagccggtg cgtcccaatt
     2281 gactggctcg attgcctacc gcccccggtt gagtcagccc cacggcgccc gagggtgcgc
     2341 cccgctgaca gagcggtcaa ccggcgggat cgacgccgtt gtcagttgct ctgcgggggt
     2401 gccgccgggt caccgggagg gaggtcgcgg tgcccgcagc gccgctgaaa gtgctcgcgc
     2461 aggcgcgcgc gctcctccgg tgtcatgttt tcccagcgct cgcgccacat gcgctgccgc
     2521 cagtgtccgt gccagccgcg accgcgcagg ccaccgaaca ggattcggct cagcaacagc
     2581 agcccgaacg cctcccggta ttgcagcggc ggtccgtgga acagctccgg gaccagcgcg
     2641 ttccacagca gcatcaccgc gagcgagagc aacgccacga ccgccgtaac ggccgccagc
     2701 accgcaacac ctttcaccga ccagcga
//
LOCUS       DATDMP010000260         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1039958, whole genome shotgun sequence.
ACCESSION   DATDMP010000260 DATDMP010000000
VERSION     DATDMP010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039958"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..461
                     /locus_tag="VI195_04425"
     CDS             39..461
                     /locus_tag="VI195_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6823659.1"
                     /translation="MSVEAETDSERLVLRFFATLSAGDLEGVRHMLHAQATWKPQMRS
                     VPGAGIHRGPGGIVDHFLTPIRALFRPGDPKILVDTIASRGDFVMIESHSGGFLADGR
                     PYENLYAWAVEVRDGKIYALREYFDSHYVMQLTAPGSK"
     gene            458..1609
                     /locus_tag="VI195_04430"
     CDS             458..1609
                     /locus_tag="VI195_04430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY6823660.1"
                     /translation="MSESLFSQERLEHLQRALAEDVAARRYYGAVMALARRGQLGIHA
                     AVGHADATAQQPLQRDSVFSIFSVTKAITNVLVLRAIELGRFALTTRISELVPAFAGG
                     LRNEITVFHLLTHTSGLPIVFAIKPDMYIDQLDEMVAAVCEHVVPAEPPGERVAYSPL
                     ANHVLLGEALRRTDPKRRSYRAIVQQDLLDPLKMRDTSVGLRRDLRTRHVVPDLRGNF
                     PITHPGHSNLGPNGAFQEEDAEMPWVGVASTVSDMLRFAEMLRRRGELDGARILSPAT
                     LELATRNWTGEKPNELTKTRYQQRGWPPEPAYMGLGFALRGTTVCHHLFGTLASPSTF
                     GNHGAGSTLYWVDPARDLSFVCLTAGVMDSLDNIERFQRLSDIAISAAT"
     gene            1621..1830
                     /locus_tag="VI195_04435"
     CDS             1621..1830
                     /locus_tag="VI195_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823661.1"
                     /translation="MKIFGPDNQELIAISAIERDGVELVLRGKILGTLPMSARIRPEE
                     ARRGLRMLDWRTVIFVLSLPWRRRR"
     gene            1848..>2141
                     /locus_tag="VI195_04440"
     CDS             1848..>2141
                     /locus_tag="VI195_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY6823662.1"
                     /translation="MPEEFLPPERIGFVGLGNMGAPMARHLVGAGYQLVAADASSAAL
                     EHFCSTVRCERARSLTELGQCCRLVITMLPDGAAVRQVLLGNDGIAAGLAPGCV"
BASE COUNT          359 a          685 c          713 g          384 t
ORIGIN      
        1 ggcgcaatga ggcgcaatga gatgcctgga gaaataccat gagtgtcgaa gccgagactg
       61 acagcgaacg actggtgctg cgcttcttcg ccaccctcag tgccggcgat ctggagggag
      121 tgcggcacat gctgcacgcg caggcgacct ggaagccgca gatgcgcagc gtgccgggtg
      181 ccggcatcca ccgtggcccc ggcggaatcg tcgatcactt cctgactccg atccgtgcac
      241 tgtttcgccc cggtgacccg aagattctcg tggacaccat tgcctcgcgg ggagatttcg
      301 tcatgattga gtcgcacagc ggcggctttc tggcggacgg ccgaccgtac gagaacctgt
      361 acgcctgggc ggtggaagtc agagacggca agatctacgc cctgcgcgaa tacttcgaca
      421 gccactacgt gatgcaactg acggcgccgg ggtcgaaatg agcgaatcgc tgttcagtca
      481 ggagcgcctc gagcacttgc agcgcgcgct cgcggaggat gtggccgcgc gccgctacta
      541 tggcgcagtg atggcactgg cgcgccgcgg gcagctcggc attcacgcgg cggtcggcca
      601 tgcggatgcg acggctcagc agccgctgca acgcgattcg gtgttcagca ttttctcggt
      661 gaccaaggcc attaccaacg tgctggtgtt gcgtgccatc gagctcgggc ggttcgccct
      721 gaccacacgc atcagcgagc tagtgcccgc attcgccggt ggcttgcgca atgagatcac
      781 ggtctttcat ctgctcacgc acacctccgg gctgcccatt gtcttcgcca tcaagccgga
      841 catgtacatc gatcagctgg acgagatggt cgcggcggtg tgcgagcacg tcgttccggc
      901 ggagccgccc ggggagcggg tggcttactc gccgctcgcg aaccacgtgt tgctcggtga
      961 ggcactgcgg cgcaccgatc ccaagcgccg ctcgtatcgc gccatcgtgc agcaggacct
     1021 gctggatccg ctcaagatgc gcgatacctc ggtgggactg cgtcgcgact tgcgcacgcg
     1081 ccacgtcgtg cccgacctgc gcggcaactt ccccatcacc catccgggac acagcaatct
     1141 tgggccgaac ggtgcgtttc aagaggaaga tgcggaaatg ccctgggtag gtgtcgcgtc
     1201 caccgtctcc gacatgctgc gcttcgccga aatgctccgc cgccgtggtg agctcgatgg
     1261 cgcacgaatt ctgtcaccgg cgacgctcga gctggcgacc cgcaactgga cgggcgagaa
     1321 accgaacgag ctcacgaaga cgcgctatca gcaacgcggc tggccgcccg aaccggccta
     1381 catgggtctg ggattcgcgc tgcgcggcac cacggtgtgt catcatctat tcggtacgct
     1441 cgcctccccc agcaccttcg gcaaccatgg tgccggcagc acgctctact gggtcgatcc
     1501 cgcgcgcgat ctgtcattcg tctgcctgac cgcgggggtc atggacagtc tcgataacat
     1561 cgagcgcttt cagcgactgt ccgatatcgc tatttctgcg gcaacctagg tgggggtggt
     1621 gtgaagatct tcgggcccga caatcaggag ctgatcgcga tctccgcgat cgagcgcgac
     1681 ggcgtggagc tcgtgctgcg cggcaagatt ctcggcacgc tgccgatgag tgcccgtata
     1741 cgaccggagg aggcgcggcg cggcctgagg atgctcgact ggcgcacggt gatcttcgtg
     1801 ttgagccttc cctggcgccg gcggcgctga ttcgaggctc gcaacccatg ccagaagagt
     1861 ttctgccacc ggaacgcatc ggctttgtcg ggctcggaaa catgggcgca ccgatggcgc
     1921 ggcacctggt cggcgcgggc taccagctgg tggcggccga tgcgagctcc gccgcgctcg
     1981 agcacttctg cagcacggtg cgctgcgagc gggcacgctc gctcaccgag ttgggccagt
     2041 gctgccgcct ggtgatcacc atgctgcccg acggcgcggc ggtacggcag gtgctgctgg
     2101 gaaatgacgg cattgcggcg ggcctggcgc ctggatgcgt c
//
LOCUS       DATDMP010000261         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2151476, whole genome shotgun sequence.
ACCESSION   DATDMP010000261 DATDMP010000000
VERSION     DATDMP010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151476"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VI195_04445"
     CDS             <1..310
                     /locus_tag="VI195_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEY6823663.1"
                     /translation="PGSATVPQARLDLSFSGTSWVEVSDAEGRRLLHGLIDGGSTRTL
                     IGSAPLLVVLGNASAVAVRLNGQTVSPGPIVRRDGSAHLLIDAAGRVSAAAPRLAHQD
                     "
     gene            312..1610
                     /gene="hisS"
                     /locus_tag="VI195_04450"
     CDS             312..1610
                     /gene="hisS"
                     /locus_tag="VI195_04450"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY6823664.1"
                     /translation="MGEQIQPVRGMNDVLPEAIGAWQYFESGTRDLLSAYGYQEIRVP
                     LLEHTELFQRAIGEHTDVVGKEMYTFTDRGGDSLTLRPEATAGIVRALISNGMLRGQR
                     HKLWCLGPMFRHEAPQAGRYRQFWQVDVEAIGCPGPDVDAELIAMSARLWRRLGIEGL
                     TLQLNSLGTPESRRLYRARLVDYLKTHEGELDGDSRRRLTGNPLRVLDSKNPDMHELI
                     RGAPLLTEHLDTESQTHFEELCAMLRAVGVPYEINPRLVRGLDYYNRTVFEWITASTG
                     AQNAVCSGGRYDGLIAQLGGDATPAVGFAMGVERLVTLLVAAGRAPAAPHPDVYVVVG
                     GPQAFTPALQLVERLRTERPAVCFELNLGGGNLKAQFRRADRSGAVLALILGEDEVAR
                     AVVGLKPLRDTAGQSECPIAQLPARLDAALAAARTAAAQG"
     gene            1624..>2322
                     /locus_tag="VI195_04455"
     CDS             1624..>2322
                     /locus_tag="VI195_04455"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6823665.1"
                     /translation="MDDYLSEKEQWEWLKAQVRENAPAAVVAILIVTVGVFGWRWWQS
                     HQDARQLQAGAKYMQMVQSLERGDRTQSLVLLGELERDYSGSPYADQARLLAARMYVD
                     EAQLDHAASELAQVAEHSKDAELAQVARLRLARVQLAQGKPDSALATLGDSAPGAFAA
                     RYHEVRGDVYYAKGDKAAALTEYRSAQTTGAEGTDSALLQLKIADLGGGAATAAPSAT
                     ANTSAPRAATPPTGT"
BASE COUNT          383 a          771 c          823 g          345 t
ORIGIN      
        1 gcccggctcg gccacggtgc cgcaggcgcg gctcgatctg tcgttttccg gcacgagctg
       61 ggtcgaggtc tccgatgccg agggtcgccg cctgctgcat ggactcatcg acggtggcag
      121 tacgcgcacg ctgatcggca gcgcgccgct gctggtggtg ctcggcaacg cctcggccgt
      181 ggcggtgcgg ctcaacgggc aaacggtgtc gcccggaccg atcgtgcggc gcgacggtag
      241 cgcgcatctg ctgatcgacg ctgccgggcg cgtcagcgcg gcggcgccgc gactcgcaca
      301 ccaggactag catgggcgag cagatccagc cggtacgcgg aatgaacgac gtactgccgg
      361 aagcgatcgg cgcatggcag tacttcgaga gcggcacgcg cgacctgctg agcgcctacg
      421 gatatcagga gatccgcgta ccgctgctcg agcatacgga gctgttccaa cgcgcgatcg
      481 gcgagcacac cgatgtcgtc ggcaaggaga tgtatacctt caccgaccgc ggcggcgaca
      541 gcctgaccct gcgtccggag gcgaccgccg gcatcgtgcg cgcgctcatc tccaacggca
      601 tgttacgcgg gcagcgtcac aagctgtggt gcctcggccc gatgtttcgc cacgaggcgc
      661 cgcaggccgg acgctaccgg caattctggc aagtggacgt cgaggcgatc ggttgcccgg
      721 gacccgacgt ggatgcggag ctcattgcga tgagcgcacg gttgtggcgg cgcctcggta
      781 tcgagggcct cactctgcag ctgaactccc tcggcacacc cgagagccgc cgcctctacc
      841 gtgcgcggct ggtcgactac ctcaagacgc acgagggtga gctcgatggc gacagccggc
      901 gccgcctcac cggcaatccg ctgcgcgtgc tcgacagcaa aaaccccgac atgcacgagc
      961 tgattcgcgg ggcaccgctg ctcaccgagc acctcgatac ggagtcgcag acgcacttcg
     1021 aggagctgtg cgcgatgctg cgcgctgtcg gggtgccgta cgaaatcaat ccacgcctgg
     1081 tgcggggtct cgattactac aaccgcacgg tgttcgaatg gattaccgca tcgaccggcg
     1141 cacagaatgc cgtttgctcg ggcggccgct atgacgggct catcgcgcag ctcggcggcg
     1201 atgcgacgcc cgcggttggc ttcgccatgg gagtcgagcg gctggtaacg ctgctcgtgg
     1261 cggcgggacg cgctcctgcg gcaccgcacc cggatgtgta cgtcgtggtc ggcggcccgc
     1321 aggcattcac gccggcgctg caactggtag agcggctgcg caccgagcgg cccgcggtgt
     1381 gcttcgagct gaacctgggg ggtggcaatc tcaaagccca gtttcgccgc gccgaccgta
     1441 gcggggcggt gctggcgctg atcttggggg aggacgaggt tgcgcgcgcc gtggtcggct
     1501 tgaaaccttt gcgcgacacg gccggtcaga gcgagtgccc gatcgcacag ctgccggcca
     1561 ggctcgacgc ggcgctcgcg gcggcacgta ccgcggcggc gcagggctga aaggggagca
     1621 tgcgtggacg attatctgtc tgaaaaagag cagtgggagt ggctcaaggc ccaggtgcgc
     1681 gagaacgcgc cggcagccgt ggtggcaatc ctcatcgtaa cggtcggtgt tttcggctgg
     1741 cgctggtggc agagtcacca ggacgcacgc cagctccagg ccggagccaa gtacatgcaa
     1801 atggtccagt cgctcgagcg cggcgatcgt acgcagtcgc tggtgctgct tggggagctg
     1861 gaacgcgatt acagcggctc gccctacgcc gaccaggcgc gtcttctcgc cgcgcgcatg
     1921 tatgtagatg aggcgcagct cgaccacgcc gcgagcgagc tcgcccaggt cgcggagcac
     1981 tccaaggatg cggagctggc acaggtcgcg cgcctgcggc tcgcgcgcgt gcagctcgcc
     2041 caaggcaagc ccgacagcgc gctcgcgact ctcggcgact ccgcaccagg agccttcgcg
     2101 gcgcgctatc acgaggtgcg tggcgatgtc tactacgcca agggagacaa ggcggcggcg
     2161 ctcaccgagt atcgcagcgc gcagaccacc ggcgcggaag gcaccgacag cgcactgctg
     2221 cagttgaaga tcgccgatct cgggggtggc gcagcgacgg ccgccccatc cgcaaccgcc
     2281 aacacgagcg cgccgcgcgc cgccactccg cccactggca cg
//
LOCUS       DATDMP010000262         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1040108, whole genome shotgun sequence.
ACCESSION   DATDMP010000262 DATDMP010000000
VERSION     DATDMP010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040108"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VI195_04460"
     CDS             <1..841
                     /locus_tag="VI195_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823666.1"
                     /translation="YAPSDYFVVLLEVFVILMFACAVAAEYLGTVFERLHAQVAAALN
                     EAERGEQLWSAVLEQLPLPAFVVDADTCEVCFASSTALAKFQGGEEALLGRSLIEALR
                     FSYPELVQELVASAGGGTRACMLRVGGRLRATEVRVQHLAQKGRRLALVVVEDTTESF
                     CVKAALDVAEHAALVVDAEGRVLAWNKPATALFPGAEVDAEISRLLPQPPPEPRWWDP
                     GLSGRRKSHVTVMQRVYQLTCSAIALPGEDERLYTVAFVPAARVAAADQDGGALTTLA
                     RRP"
     gene            838..1659
                     /locus_tag="VI195_04465"
     CDS             838..1659
                     /locus_tag="VI195_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823667.1"
                     /translation="MTAATTPMTPGLVLARAAPLALGVTLLLARPLIATAAEDKLVFS
                     GNGMTLTGDHGGGGAAATWIRQFDSGSLFGLGLEYQQIYNSHWTNNTFNGALKLGTDI
                     KTSLYAEAHLGSGATAGEPFDYRNIAGGFISAVTPWLSVQLEERYIDIEPSHGNLPKI
                     GLSFQLDPKVLGSVSYAQSFGGNLGTKLGTARLDYASTEFSWLLGAAYGPVSPRVLNL
                     IGQEVGPPKTLKEGFVGAGKSFGRTDWLLIGDYQDLEGFKRTTVTLNCTVHLGKR"
     gene            1675..>2320
                     /locus_tag="VI195_04470"
     CDS             1675..>2320
                     /locus_tag="VI195_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823668.1"
                     /translation="MRLDVLRRHSYWLLPLLAATLVALLGAQLIRLSVHERSEQLRQS
                     AQSAVLRHASLIEAELATLAAAAQREAGSAGAGASESATPGHGTFWMSAEGAVLRSSP
                     GDAAIASAVAREWVASDATRRAAVLFGPVRYGSQWIVAARVARADGNSPATPAWSVSY
                     EDLEPLLARARFGALVNEGYDFQLSQSDALTQHPRVFLSSQPGTLAQPATGTIRQ"
BASE COUNT          374 a          744 c          795 g          407 t
ORIGIN      
        1 ttacgcgccc tccgattatt tcgtggtgct gttggaagtg ttcgtcatcc tgatgttcgc
       61 ctgcgccgta gccgcggaat acctgggcac ggtgttcgag cgtctgcacg ctcaggtggc
      121 cgccgcactg aacgaggccg agcgcggcga acagttgtgg tcggcggtgc tcgagcaact
      181 gccgctgccg gcattcgtgg tggacgccga tacctgcgaa gtctgctttg ccagcagtac
      241 cgcgctcgcg aaattccagg gcggtgagga ggctctcctc gggcgcagcc tgatcgaggc
      301 actgcggttc tcctatcccg agctcgtgca ggagctggtc gccagtgcag gcggcggcac
      361 gcgcgcctgc atgctgcggg tcggcggccg gctgcgcgcc accgaagtgc gcgtgcagca
      421 cctggcgcag aagggccggc ggctcgctct ggtggtggtg gaggatacga ccgaaagctt
      481 ctgcgtgaag gccgcgctcg atgtcgccga gcatgccgcg ctcgtggtcg atgcagaagg
      541 ccgggtgctg gcgtggaaca agcccgccac ggcgctgttt ccaggcgccg aggtcgacgc
      601 cgagatatcg cgtctcctgc cgcagccgcc ccccgagccg cgctggtggg acccgggtct
      661 cagcggtcgc cgcaagtcgc acgtcacggt gatgcaacgg gtctatcagc tcacgtgctc
      721 ggcgattgct ctgcccggag aggacgagcg gctgtacacg gtcgccttcg tgccggccgc
      781 gcgggttgca gccgcggatc aggacggggg ggcgttgaca acgctcgcca ggcgcccatg
      841 actgcagcga ccacaccgat gacaccggga ctggtgctgg cgcgcgctgc gccgctcgcc
      901 ttgggtgtaa cgctgttact cgcccgcccg ctcatcgcga ccgcggcaga ggacaaactg
      961 gtgttctccg gcaatggcat gactcttacc ggcgatcacg gcggcggtgg cgccgcggcg
     1021 acctggatca ggcagtttga tagcggcagc ctcttcgggc tcggcctgga gtaccaacag
     1081 atctacaact cgcactggac gaacaacacg ttcaacgggg cactgaagct cgggacggac
     1141 atcaagacga gcctgtacgc cgaggctcac ctggggtcgg gtgctaccgc gggtgagcca
     1201 tttgattacc ggaatatcgc gggtggcttc atcagcgcag tcacgccctg gctctcggtg
     1261 cagctggaag agcgctacat agacatcgag ccgagccacg ggaatcttcc gaagatcggt
     1321 ttgtcgttcc agcttgaccc caaggtcctc gggtcggtgt cgtatgcgca atccttcggc
     1381 ggcaacctcg gcacgaagct cggcacggcc cgtctcgatt acgccagcac cgagttctcc
     1441 tggcttctgg gcgcggccta cggaccggtc tcgccccggg tgctgaacct gatcggtcag
     1501 gaggtgggac ctcccaagac actcaaggaa ggcttcgtcg gcgccggcaa atccttcggt
     1561 cgcaccgatt ggctactcat cggtgactat caggacctgg agggattcaa gcgcaccacc
     1621 gttacgctca actgcaccgt gcatctaggt aaaaggtaag cggagtacag ctgcatgagg
     1681 ctcgatgtgc tacggcgtca ctcctactgg ctgctgccac tgttggcggc gacgctggtg
     1741 gcgctgctcg gtgcgcagct catcaggctc agcgtgcacg agcgttccga acagctgcgg
     1801 caatcggctc agtcggctgt gctgcgccat gcgagcctga tcgaagcaga gctcgccaca
     1861 ctcgccgccg cggcccaacg ggaagccggc agcgcggggg ccggggcgag tgagtctgcg
     1921 acaccggggc acggcacgtt ctggatgagc gccgagggtg cagtgctcag gtcctcgccg
     1981 ggcgatgccg ccatcgcgag tgcggtagcg cgcgagtggg ttgccagcga cgccacacgg
     2041 cgtgccgcag tattgtttgg accggtgcgc tatggcagcc agtggatcgt tgcggcgcgc
     2101 gtcgcgcgag cggatggcaa ctcgccagcg acgcctgcct ggtcggtgag ttacgaagat
     2161 ctcgagcccc tgctggcgcg cgcccgcttc ggggccctgg tgaatgaggg ttacgacttc
     2221 cagctgtcgc agtccgatgc gctcacgcag cacccgcggg tatttctcag ctcacagccc
     2281 ggaacgcttg cacagcctgc caccggcacc attcgccagc
//
LOCUS       DATDMP010000263         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1818057, whole genome shotgun sequence.
ACCESSION   DATDMP010000263 DATDMP010000000
VERSION     DATDMP010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818057"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VI195_04475"
     CDS             <1..680
                     /locus_tag="VI195_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY6823669.1"
                     /translation="RNEFQYADAVGTVEAFRAFLARHPSGAWSERARIRIESFSLGSA
                     PRMGPLTPEEAATFSESRVRALRLATAAAAQGADASTPAAAVQPAALPLGGAPSATGA
                     ALPEVVDAQAESATGRAVPGEAAQAATDSVVTPVAATEVGTQSGYGIQLGAFGSEASA
                     DREWRRLQGRFGAELGNLSPRIVIAESDSRQLYRLQAPASGEAQARAICDSLREQAQA
                     CIPVVPR"
     gene            complement(968..1327)
                     /locus_tag="VI195_04480"
     CDS             complement(968..1327)
                     /locus_tag="VI195_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6823670.1"
                     /translation="MELASSSRRHGIGGPAMYLSRSPPLKNLRAFCVAARHLSFKFAA
                     AELCLTPSAVSHQMKELEVLLGVRLFERRTRALELTATGRRLLAAVEPLLEGLERVLT
                     QLSHRDGRGTLRLRAPP"
     gene            1476..>2360
                     /locus_tag="VI195_04485"
     CDS             1476..>2360
                     /locus_tag="VI195_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY6823671.1"
                     /translation="MEFRDYYKILGVERSATADQIKSAYRRLARKYHPDVSKEPNAEA
                     HFKEMQEAYEVLRDPEKRTAYDQLGSEWKSGQSFHPPPNWGSGFEFSGRPAGAGGGRR
                     YHAGRAPGGSSVGEEDFSDFFSSLFGGGSPFAGAAGRAGGRDHHARVDITLDEAYRGT
                     ARMLELKRPEVKPDGSVELRSHTVRVAIPPGVTEGQLIRLAGQGEPASGGGSAGDLYL
                     EAHILPDKVFALDNRDVTIAFPVAPWEAALGASVTVPTLGGPVEMRIPAGSQSGQKLR
                     LRGRGLPGHSAGDQYVQLK"
BASE COUNT          398 a          771 c          809 g          382 t
ORIGIN      
        1 tccgaaacga gtttcaatat gccgacgctg ttggaacggt cgaggccttc cgagccttcc
       61 tcgcgcgtca cccgagtggg gcctggtccg agcgtgcgcg catccgcatc gagtccttct
      121 cactcgggtc agcgccgcgt atggggccac tgactcccga ggaggccgcc accttcagcg
      181 aatcgcgtgt gcgggcgctg cgcctcgcga cggccgcggc agcgcaggga gccgatgctt
      241 ccacgccggc cgctgccgtg caaccggcgg cgctgccact cggcggtgct ccctccgcca
      301 ccggtgcggc gttgcccgaa gtggtggatg ctcaggcgga gagtgcaaca ggccgggccg
      361 tgccgggcga ggcagcacag gccgccaccg actcagtggt caccccagta gcggcaaccg
      421 aggtaggcac gcagagcgga tatggcatcc agctcggcgc gttcggaagc gaggcgagcg
      481 ccgatcgcga gtggcgccgg ctccaggggc gcttcggcgc cgagctcgga aatctgtcac
      541 cgcgaatcgt gatcgccgaa agcgactccc gtcagctcta tcgcctgcaa gcccccgcct
      601 cgggcgaggc gcaggcccgt gccatttgcg attcactccg ggagcaggcc caggcctgca
      661 taccggtcgt cccacgctga tcctggccca ctacgcgcat gacacctggg ccgacggcca
      721 caggtggcta gacactgctg ccgcgggctt caaagtgaca atttccgtca ctcaaacggt
      781 ctgaccgatc gaaagataac cacatgattt tcatgagatt catgcgttgg cacaagcttt
      841 gctgagtata gctcagttgc gaacaattcc ccgtgttcgc tcctgcgcgc cgcgatgcga
      901 cttcccccgc ttcgcggcgc tttttttctt gggccgatcg gccgggctct gggagtgcag
      961 ggccctgtca ggggggcgca cgcaagcgca gtgtcccgcg gccatcgcgg tgggagagtt
     1021 gcgtcaatac gcgctcgagt ccctcgagca gcggctccac tgccgcaagc aggcgccggc
     1081 cagtcgcggt gagctcgagg gcgcgcgtgc gccgctcgaa caagcgcacg ccaagcagta
     1141 cctcgagctc cttcatctgg tggctgaccg ccgagggcgt caggcacagc tcagcggctg
     1201 cgaatttgaa gctcagatgg cgcgcagcca cgcagaacgc ccgcaaattc ttcaggggcg
     1261 gggatcggga aagatacatc gcggggccac caatgccgtg ccgtctgctt gaggaagcaa
     1321 gttccatgcc ccatgtaccg ctgagcagga ggcgtgcctc aagccgcagc ggcgcctgct
     1381 tgccctgaag cggtgcaggt ggcgcgcgtc cgccccgagt cggtgcacgc tgcggataga
     1441 atcgcgcaag ccgttcgggt gcggcggggt gttggatgga attccgggac tactacaaga
     1501 ttttgggcgt cgagcgctct gccaccgccg accagatcaa gagcgcgtat cggcgcctcg
     1561 cgcggaagta tcaccccgat gtcagcaagg agccgaacgc cgaagcgcac ttcaaggaaa
     1621 tgcaggaggc gtacgaagtg ctgcgcgatc cggagaaacg caccgcgtat gaccagcttg
     1681 gcagtgagtg gaaatcggga cagtccttcc acccgcctcc gaactggggg agtgggttcg
     1741 agttctccgg ccgtccggcc ggtgcgggtg gcggccggcg ttaccatgcc ggcagagccc
     1801 ccggcggcag cagcgtcggt gaagaggatt tcagcgactt cttctcctcg ctattcggcg
     1861 gcggctcacc ctttgcaggg gcggccggcc gcgcgggcgg gcgcgatcac catgcgcgcg
     1921 tcgatatcac cctcgatgaa gcctatcgcg gtaccgcgcg gatgctggag ctcaagcgcc
     1981 ccgaggtgaa gccggatggc tcggtggagc tgcgctccca caccgtgcgc gtcgcaattc
     2041 caccgggcgt caccgaaggc cagctgattc ggcttgcggg ccaaggggag ccggcgagcg
     2101 gcgggggcag cgcgggagat ctgtacctcg aagcgcatat cctgccggac aaagttttcg
     2161 ccctcgacaa ccgcgacgtg acgatcgctt ttccggtggc cccctgggag gccgcgctcg
     2221 gagcgagcgt cacggtgccg accctcgggg ggccggtcga gatgcgcatt ccggctggat
     2281 cgcagtccgg ccagaagctg cggctgcgtg gtcgcgggct cccgggacat tcagcggggg
     2341 atcaatacgt gcaactgaag
//
LOCUS       DATDMP010000264         2993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_818624, whole genome shotgun sequence.
ACCESSION   DATDMP010000264 DATDMP010000000
VERSION     DATDMP010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818624"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1676)
                     /locus_tag="VI195_04490"
     CDS             complement(123..1676)
                     /locus_tag="VI195_04490"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HEY6823672.1"
                     /translation="MAELLRKGHFLGTKLRSVRKRNGLTLEELSARCIQRNARIAPSV
                     SYLSMIESGKRSPSPALLELLAAVFQRPPAWFLDESTQSEPVAPPASAPGGAARVPLE
                     PAFLFSKSLLQAAIPELLAQTGTSGRQFAHLLIRSHQEMSRNDFPDLERAADSVGERR
                     FPLAVEDLMRLSRRHGLEIRWFDRKPVLARDKDREVRSMVRSFFEAPGIVYANRALQS
                     DPARLKFDLAAHIGHKLLHGGDGLKSPHATGGEMGGSPEGGAAGAGLDARDVLHAWRD
                     FECTFFAGALLAPRVPFRRFLAHERYRVEAAARLELTPAVVMRRMTKVSPYPYWHFFD
                     AYPPGYLRAVYRGNGIPLPWGNLAQVSDPCPHWAVFRMISDQRGESGSQISVLRDGER
                     SLLYCCHSVRVRDMAGNPHVLSVGVDLAPALDANGAASAPMIEAIFRECLHRRGEARI
                     PRDAVQALGAVASVLNIAWIEDALLSPARIICPRSTRCPRPERCEGAPPSRAREIADV
                     REEILGRES"
     gene            1769..>2993
                     /gene="aceB"
                     /locus_tag="VI195_04495"
     CDS             1769..>2993
                     /gene="aceB"
                     /locus_tag="VI195_04495"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY6823673.1"
                     /translation="MQTGAQERVYSREPAGPRVEILGARIERSDEALTPLALQLLAHL
                     HRRFNPRRLELLEARAARQAQFDAGALPEFLPGTAAVRAGEWRVAPVPDDLADRRVEI
                     TGPVDRKMIINALNAPVRAFMADFEDSCSPTWENVIRGQVNLRDAVRRTISHEDPATG
                     KVYRLAERTATLIVRPRGWHLPEKHVRIGGEAVSGALFDFAMFLANNHEALARRGTGP
                     YFYLPKIESHLEARLWNEVFLAAQDALAIPRGTIKATVLIETLPAAFEMDEILHELRE
                     HSAGLNCGRWDYIFSFIKKFRNRPDFVLPDRATVTMERHFLKSYVELLIRTCHRRGAH
                     AMGGMAAQIPIRDDPQANEAAMARVRADKLREARAGHDGTWIAHPGLAAVAREPFDQV
                     MHGPNQLGALRQDVQV"
BASE COUNT          449 a         1059 c         1072 g          413 t
ORIGIN      
        1 cggcgcaccg ccgcccgtct ccgcggcgcc cgcggcaagc gacagcgcca tcgccagcgc
       61 cagtgccgcg atccgtccgc gcgttgcctc gagcccctgc atgatggggc attctaaggc
      121 catcagctct cgcggccgag gatctcctcg cgcacgtcgg cgatctcgcg tgcgcgcgag
      181 ggcggcgcgc cctcgcagcg ctcggggcgc gggcagcggg tgctgcgcgg gcagatgatg
      241 cgcgccggcg acagcagggc gtcttcgatc caggcgatgt tgagcacgct ggcgaccgcg
      301 ccgagcgcct gcacggcgtc cctggggatg cgcgcctcgc cgcgccggtg caggcactcg
      361 cggaagattg cctcgatcat cggcgcgctc gccgccccgt tcgcatcgag cgccggcgcc
      421 agatccaccc ccaccgaaag cacgtgcggg ttgccggcca tgtcgcgcac ccgcaccgag
      481 tggcagcagt acagcagcga gcgctcgccg tcgcgcagca ccgagatctg cgagcccgat
      541 tccccgcgct ggtcggagat catgcggaac accgcccagt gggggcaggg gtcgctcacc
      601 tgggcgaggt tcccccaggg cagcggaatg ccgttgccgc gatacaccgc gcgcaggtag
      661 ccgggcgggt aggcgtcgaa gaaatgccag tagggatacg gggagacctt ggtcatgcgc
      721 cgcatgacga ccgcgggcgt caactcgagc ctggcggccg cctcgacccg gtagcgctcg
      781 tgcgccagga agcgccggaa cggcacccgc ggggcaagca gcgcgccggc gaagaaggtg
      841 cactcgaagt cgcgccaggc gtgcagcacg tcccgggcgt cgagcccggc gccggccgcg
      901 ccgccctcgg ggctgccgcc catctccccg ccggtcgcat gcggggactt gagcccgtcg
      961 ccgccgtgca gcagcttgtg gccgatgtgc gcggccaggt cgaacttgag gcgggccggg
     1021 tcggactgca gcgcgcggtt cgcgtacacg atccccgggg cctcgaagaa ggagcgcacc
     1081 atgctgcgca cctcgcggtc cttgtcgcgc gccagcaccg gcttgcgatc gaaccagcgg
     1141 atctcgagcc cgtggcggcg gctgaggcgc atcaggtcct cgaccgcgag cgggaagcgc
     1201 cgctcgccca cgctgtcggc ggcacgctcg aggtcgggga aatcgttgcg cgacatctcc
     1261 tggtgggagc ggatcaggag gtgggcgaac tgccggccgc tcgtgccggt ctgcgcgagc
     1321 agctcgggga tcgccgcctg cagcaggctc ttggagaaca ggaacgcggg ctcgagcggc
     1381 acccgtgccg ccccgcccgg ggccgaggcg ggcggcgcga cgggctcgct ctgcgtgctc
     1441 tcgtcgagga accaggcggg ggggcgctgg aagaccgcgg cgagcagctc gagcagcgcg
     1501 ggggaggggc tgcgtttgcc gctctcgatc atgctgaggt aggacaccga aggggcgata
     1561 cgggcgttgc gctggatgca gcgcgccgag agctcctcca gcgtcagtcc gttgcgcttg
     1621 cggaccgagc gcagcttggt gccgaggaag tggcccttgc gcagcagttc cgccatgggg
     1681 tcctcgcggt gggtacgggg cgcgtgtgaa attaacagtg tgaagtttcc atagtcaaat
     1741 ttgtagccgg tacatgtatg ctggagccat gcagaccggt gcgcaggagc gggtctactc
     1801 gagggagcct gccggaccgc gggtcgagat cctcggggcg aggatcgagc gctcggacga
     1861 ggcgctgacg ccgctcgcgc tgcagctgct cgcccacctg caccgtcgct tcaatccgcg
     1921 gcgcctcgag ctgctcgagg cgcgcgccgc gcgccaggcg cagttcgacg ccggggcatt
     1981 gccggagttt ctgcccggca ccgcggcggt gcgcgcgggc gaatggcgcg tcgcgccggt
     2041 gccggacgat ctcgccgacc gccgcgtcga gatcaccggc ccggtcgatc gcaagatgat
     2101 catcaacgcc ctgaatgcgc cggtgcgcgc cttcatggcg gacttcgagg actcctgctc
     2161 gcccacctgg gaaaacgtga ttcgcggcca ggtgaacctg cgcgatgcag tgcgccgcac
     2221 gatcagccac gaagacccgg ccaccggcaa ggtctaccgc ctggccgagc gcaccgccac
     2281 cctcatcgtg cggccgcgcg gctggcatct gcccgagaag cacgtgcgca tcggcggcga
     2341 ggccgtctcc ggcgccctgt tcgacttcgc catgttcctc gccaacaacc acgaggcgct
     2401 cgcgcgccgc ggcaccggcc cgtacttcta cctgcccaag atcgagagcc atctcgaggc
     2461 gcggctgtgg aacgaggtgt tcctcgccgc ccaggatgcg ctcgccatcc cgcgcggcac
     2521 catcaaggcc acggtgctga tcgagaccct gcccgcggcc ttcgagatgg acgagatcct
     2581 ccacgagctg cgcgagcatt ccgccggcct caactgcggc cgctgggact acatcttcag
     2641 cttcatcaag aaattccgga atcgccccga cttcgtgctg ccggaccgcg ccacggtcac
     2701 catggagcgg cacttcctca agtcctacgt cgagctgctg atccgcacct gtcaccggcg
     2761 cggcgcgcac gccatgggcg ggatggccgc acagatcccg atccgcgatg acccgcaggc
     2821 caacgaggcc gccatggcgc gggtccgcgc cgacaagctg cgcgaggcac gcgccggaca
     2881 cgatggcacc tggatcgcgc atccggggct ggcggcggtg gcgcgcgaac ccttcgacca
     2941 ggtcatgcac ggccccaatc agctgggcgc gctgcgccaa gatgtgcagg tga
//
LOCUS       DATDMP010000265         7173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_151834, whole genome shotgun sequence.
ACCESSION   DATDMP010000265 DATDMP010000000
VERSION     DATDMP010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151834"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VI195_04500"
     CDS             complement(<1..892)
                     /locus_tag="VI195_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823674.1"
                     /translation="MKNLVSAVAAPTRQICPARCGWLRQACAVAALLVLVLLDPRSAQ
                     AQPPVLQRGYDAGVSGATLNETTLTTSNVTSNTFGLVYKLTVDDNIMAQPLYVPNIVI
                     NGTSHNVVYIATMSDTLYAFDADVGGAPLWTLNLASFEHATPVPIAQFAYSGNKNIIN
                     NLGILSTPVIDLPTNMLYAVACTLENNTLVYRLHAVNIATGVPRTGSGVVISGTYGAL
                     TFNARNQWQRVSLTFSGNNVVFAFGALELESTGNPYVGWVMAYDKTSLMQTGIYATVT
                     TGNGGGGVWQSGRPPAVDASG"
     gene            complement(1085..3535)
                     /locus_tag="VI195_04505"
     CDS             complement(1085..3535)
                     /locus_tag="VI195_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6823675.1"
                     /translation="MPNNSPPSHARAVIIGGGIAGCSIAYHLAKLGWTDVVLLERAQL
                     TSGTTWHAAGLLTTLRDTETQTRLAAYTQQLYRRLEEETGQATGLIECGSIQLALTAA
                     KTQEMRRGYRAAQSWGIECAEISAAEVQRLWPLAEVGDVQAAFHFPSDGRINPTDVTR
                     ALAKGARQAGVGIFEKCPVTAIEQHDGRVTAIESALGRIVTDTVVNCAGMWAPQIGRM
                     AGVDVPLQAAEHYYLISEPIAGVHSKLPILRDPGRSTYIREEAGKLMVGIFEDVARPW
                     KVAGIPEDFSFGEIPPDWERMQPYLERACGRVPVLAHTGVKLLFCGPESFTADHNYLM
                     GEAPNLKGFFVAAGFNSLGILSGGGVGYVMAHWIAHGNAPLDVWSVNIRRTHAWQNNR
                     RYLADRVVETLGIGYQDHWPYRQWSTARGIKRSMLHDRVAAAGACFGESAGWERPNWY
                     ARPGQQPVYEYAWGRQNWFENNAQEHRAVREAVGLFEQSSFAKILVQGRDAERALDVI
                     ATADCRVPIGKIRYAQFLNARGGIEADVSITRLATDRFMVVTAAFTATHVIAWLRQHI
                     AVDWHCVVTEVSDAWCMLNVQGPRSRELLSSVADGDWSNEGFPFGTAREVQIGYQQAL
                     ALRITYVGELGWELYIPVPCAIGVYDAIVEAGKPLGLRHCGYHALNSLRIEKAYREWS
                     HDIGPDDTPLDAGLAFTCAWTKPEGFIGRDALLAARSQPRRRRLVQFLLSDPSAMLYY
                     NEPILLEGKRVGLVTSGMFAHTLGAAAALGYVSQEQGVTDELIASAGFEILIGDQPVP
                     ARASLRPLYDPAGLRSRS"
     gene            complement(3548..4165)
                     /locus_tag="VI195_04510"
     CDS             complement(3548..4165)
                     /locus_tag="VI195_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6823676.1"
                     /translation="MSPASSGWYLRRVIVQALCALLLGLRPSPPTSAQDTKPLTAILL
                     IAREELRDPNFTDSVVLVMNHLGPAPVGLVVNRPTEVPVSRLFPDLKRLAPLHDKVYF
                     GGPVELASVWFLFRAAHPPEHAVQAFGNIYLSANRDLLLQLLGRDKPMDDLRIFIGHS
                     GWGPGQLETEIAQGSWRLERADADAIFKGKSEHPWPAPEHPDNST"
     gene            4598..5794
                     /locus_tag="VI195_04515"
     CDS             4598..5794
                     /locus_tag="VI195_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6823677.1"
                     /translation="MIIMIVAVLALIGIIAGIKVLMIMKMLSGMKPPPPAVVTTAKTS
                     YQQWQPELRAVGTLRAARGADLALEVAGLVGRVNVRSGEDVKSGQLLLELVTTDEVAN
                     LHQLEAQAALSQLTYERAKQQIAVHVIAQADYDNAAADLKVKQAAIAQQQAIIAKKQL
                     RAPFAGRAGIVTITPGTYLNSGTVIVTLQELDPVYVDFFVPQKDLAQAQVGRNATLTL
                     DAFPGKGFRGTVNAVNPKVDTDTRNVLVEARVPNPDRVLSPGMFVNVAVEVGSQQRYL
                     TLPQTAIVYNPYGETVYIVRTKSQFDAEQAAAAAKTADPVDKDPPKKKGKGPPPIPAD
                     ALVVEQTFVTTGPTRGDQVAVLSGLDAGVEVVSSGQIKLKNGAPVRIDNSVKPADNPH
                     PTPQEH"
     gene            5835..>7173
                     /locus_tag="VI195_04520"
     CDS             5835..>7173
                     /locus_tag="VI195_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6823678.1"
                     /translation="MNFTDIFIRKPVLAIVVSSLIFVLGLRALTGLPVRQYPQTQNAV
                     VTITTAYYGADAQTIAGFITQPLEQAVAQAQGIDYLSSASATGLSTITATLRLNYDAN
                     RALTEIQTQVTSVRNQLPPQAQQPVLNIAIGQSLDAMYMGFRSDVLPSNSITDYLARV
                     VKPKLDALPGVQTAEILGGRQFALRAWLNPAQLAAHGVTAADVFQALGTNNYLAAVGT
                     TKGQMVSVDLTANTDLHTVEEFRQLAIKQKNGAIVRLQDVATVVLGAEDYDSNVAFSG
                     KLAVFIGVKAAPDANLLDVAQRVRTALPDIQSQLPRGLQGTIVYDSTGFVTSSIREVV
                     KTLIEALLIVTGVIFVFLGSLRAVVIPVIAMPLSLIGTFFVMLALGYSMNLLTLLALV
                     LAIGLVVDDAIIVVENVDRHMKEEGKSPLEASLLAARELGGPILAMTVVLVAVY"
BASE COUNT         1270 a         2346 c         2347 g         1210 t
ORIGIN      
        1 agcccgaagc atcgacggcc ggcggccggc ccgactgcca tacgccaccg ccaccattac
       61 ccgtcgtcac cgtggcgtag ataccggtct gcatgaggct ggttttgtcg taggccatca
      121 cccagccgac gtaggggttg ccggtcgact cgagctcgag cgctccaaat gcgaacacca
      181 cgttgttgcc ggagaaggtg agcgataccc gctgccactg gttgcgcgca ttgaacgtca
      241 gcgcgccgta ggtccccgaa atcaccacac ccgaacccgt gcgcggcaca cccgtggcga
      301 tattgaccgc gtgcagccgg tacaccagtg tgttgttctc cagcgtgcag gcgacggcgt
      361 agagcatgtt ggtggggaga tcgatcaccg gtgtgctgag gatgccgagg ttgttgatga
      421 tgttcttgtt gccggagtag gcgaactggg cgatcggcac cggggtggcg tgctcgaagc
      481 tggccagatt gagtgtccac agcggtgcac cgcccacatc cgcgtcgaac gcgtagagcg
      541 tgtcgctcat ggtcgcgatg tacacgacat tgtgggaagt gccgttgatc acgatgttag
      601 gtacgtacaa cggttgggcc atgatgttgt cgtcgaccgt cagcttgtac accagcccga
      661 aggtgttgct agtgacgttg gaagtggtga gcgtcgtctc attgagcgtt gcaccggaga
      721 ccccggcgtc atagccgcgc tgcagcaccg gcggttgagc ttgcgcactg cgaggatcga
      781 gcaggacgag caccagcaac gctgcgacgg cgcacgcctg gcgcagccat ccgcagcgtg
      841 ctggacaaat ctgccgggtc ggtgcagcga ccgcagacac gaggttcttc atgttgtttc
      901 tccccgcgcg ccgtcccgaa gtcccgccag ctacgagctc catgctcgcc gggcagcgca
      961 gtagttctta cgtaggactt tagtccctgg gctggagctg cacaatattc agccgctcga
     1021 ggagaagatg agtagccggg cagggcctat ctgccggcga attaagtcaa ttgcacggct
     1081 cgcgctagct gcgcgacctg agcccggcgg gatcgtagag gggccgcagc gaggcgcgcg
     1141 cgggcaccgg ctggtcgccg atcaggatct cgaagcctgc ggaggcgatc agctcatccg
     1201 ttacaccctg ctcctggctc acgtagccga gtgcggccgc ggcgcccagg gtgtgcgcga
     1261 acatgccgga ggtgacgagg cccacccgct tgccctcgag cagtatcggc tcgttgtagt
     1321 agagcatcgc cgaggggtcg gacagcagga actgcaccag gcgccgccgg cgcggctgcg
     1381 agcgcgccgc gagcagcgcg tcgcgaccga tgaacccttc cggcttcgtc catgcgcagg
     1441 tgaaggcgag tccggcatcg agcggtgtgt cgtcgggacc gatgtcgtgt gaccactccc
     1501 ggtaggcctt ctcgatgcgc agggagttga gcgcgtggta gccacagtgg cgcagaccga
     1561 gcggcttgcc cgcttccacg atggcgtcgt acacgccgat ggcgcaggga acgggtatat
     1621 acagctccca tccgagctcg cccacataag tgatgcgcag cgcgagtgcc tgctggtaac
     1681 cgatctgcac ctcgcgtgcc gtgccgaaag ggaatccctc attcgaccag tcgccgtcag
     1741 ccacggatga cagcagctcg cgcgagcgtg gaccctgcac attgagcatg caccaggcgt
     1801 cgctcacttc cgtgacgacg cagtgccagt ccaccgcgat gtgctggcgc agccaggcga
     1861 tgacgtgcgt agcggtgaac gctgcagtca ccaccataaa tcggtcggtg gcgagtcgcg
     1921 tgatactgac gtccgcctcg attccgcccc gtgcgttgag gaactgcgcg tagcggatct
     1981 tgccgatcgg cacgcggcag tcggcagtgg caatgacatc gagcgcacgc tcggcatcgc
     2041 gtccctgcac gagaatcttc gcaaacgagg actgctcgaa cagtccaacc gcctcgcgca
     2101 ccgcgcggtg ctcctgcgca ttgttctcga accagttctg ccgcccccag gcgtactcgt
     2161 acaccggctg ctgaccgggc cgcgcatacc agttgggtcg ctcccaacct gcggactcac
     2221 cgaagcaggc acccgccgcc gcgactcgat cgtgcagcat gctgcgcttg atgccgcgcg
     2281 ccgtgctcca ctggcggtac ggccagtggt cctggtagcc gatgccgagc gtttccacca
     2341 cgcggtcggc gaggtagcgc cgattgttct gccaggcatg ggtgcgacgg atgttgaccg
     2401 accagacatc gagtggcgcg ttgccgtgcg cgatccagtg cgccatgacg tagccgacgc
     2461 cgccgcccga aagtatcccg agcgagttga agcccgcggc gacgaagaag cccttgaggt
     2521 tcggcgcttc gcccatcagg tagttgtgat cggccgtgaa ggattccggc ccgcagaaca
     2581 gcagcttgac gccggtgtgc gccagcaccg gcacgcggcc gcaggcccgc tcgagatacg
     2641 gctgcatgcg ttcccagtcc ggcgggatct ccccgaagga gaaatcctcg gggatgccgg
     2701 cgaccttcca cgggcgagcc acgtcctcga agatgccgac catcagcttg cccgcttcct
     2761 cgcgtatgta ggtgctgcgg cccgggtcgc gcaggattgg cagcttcgag tgcactcccg
     2821 cgatcggctc ggagatcagg tagtaatgct cggccgcctg caggggcaca tcgacgcccg
     2881 ccatgcggcc gatctgcggg gcccacatgc cggcgcagtt gaccaccgtg tcggtaacga
     2941 tccgcccgag cgcgctctcg atcgcggtca cgcgcccgtc atgctgctcg atggcggtga
     3001 ccgggcattt ctcgaatatg ccgacgccgg cctggcgcgc tcctttggcg agcgcacgcg
     3061 tcacgtcggt cggattgatg cgaccgtcgc tcggaaagtg aaacgcggcc tgcacgtcgc
     3121 cgacctcggc caggggccac agccgctgca cctccgcggc gctgatctcc gcacactcga
     3181 tgccccaact ctgcgcggcg cgataaccac ggcgcatctc ttgcgttttc gcggccgtga
     3241 gggcgagttg gatcgaaccg cactcgatga gcccggtcgc ctgcccggtc tcttcctcca
     3301 ggcgccgata gagctgctgg gtgtaagcgg ccagccgcgt ctgggtctcc gtgtccctga
     3361 gcgttgtgag caggccggcc gcgtgccagg tggtgccgga ggtgagctgt gcgcgctcga
     3421 gcagcacgac atccgtccaa ccgagcttgg cgagatggta ggcgatcgaa catccggcga
     3481 tacccccgcc gatgatcacc gcgcgggcat ggctcggagg cgagttgttc ggcatcggcg
     3541 ctaactctca ggtgctgttg tcgggatgct cgggtgcggg ccacgggtgc tcggacttgc
     3601 ccttgaagat tgcatcggca tccgctcgct cgagccgcca tgatccctgg gcgatctccg
     3661 tctcgagctg ccctggcccc cagccggagt gcccgatgaa gatgcgcaga tcatccatcg
     3721 gcttatcgcg gcccagcagc tgcagcagca agtcccggtt ggcgctcagg tagatgttgc
     3781 cgaaagcctg gaccgcgtgc tccggcgggt gggcggctcg aaacaggaac cacaccgaag
     3841 ccagttcgac cggaccgccg aagtacacct tgtcgtgcag cggcgcgagg cgcttgagat
     3901 ccgggaacag ccgcgagacc gggacctcgg tcggccggtt gacgaccagt ccgaccggcg
     3961 cgggacccag gtggttcatc accagcacca ccgagtcggt gaaattcgga tcgcgcagct
     4021 cctcgcgagc aatgaggagg atggccgtaa gcggcttggt gtcttgcgcg ctcgtgggcg
     4081 gggagggccg gagcccaagc aacagtgcgc acagcgcctg cacgatgacg cgccgtaagt
     4141 accaaccgct actcgctggc gacaccggca tgcgcctcgc atcgcagacc accgcagagc
     4201 gattttatgc cctcggacct caccgtgcag gctggcccga ccggtctgcc gacagctgcg
     4261 ggatcccaga cggcgtcaag ccgttccacc gccgttttgc gtcccctggt cgaggctcgc
     4321 gtgcgcagcg gacaggcttc ggccgcgtcc gggagcggca tgcagtgtcg gggactcgcc
     4381 cggcctgtat ggtgcagcca tctacttctt tagagaggga cagtcagtgt cacggcgcgg
     4441 cacaatagcc cctcagggct gccgcggccg ttgaccgcgg ctcatccggg actttagagc
     4501 ttgcaacgcc gtgcgcgcgg acacggtagt cacttcgcgt taaggagcct cccgcatgca
     4561 gacgcgggtc gagaataagc caagcacgag gaagcgcatg atcatcatga tcgtcgccgt
     4621 gctcgcgttg atcgggatca tcgcgggcat caaggtgctg atgatcatga agatgctctc
     4681 cggcatgaag ccgccgccgc cggcagtggt aaccaccgcc aagacctcct accagcagtg
     4741 gcagccggag ctgcgcgcag ttggcacgct gcgcgcggcg cgcggcgccg accttgccct
     4801 cgaggttgcc ggcctcgtgg ggcgcgtgaa cgtgcgctcg ggcgaggacg tcaagagcgg
     4861 tcagctgctg ctcgagctgg tcaccacgga cgaggtggcc aacctgcacc agctcgaggc
     4921 acaagccgca ctctcccagc tcacctacga gcgcgccaag caacagatcg ccgtgcacgt
     4981 gatcgcgcag gccgactacg acaacgccgc ggccgacctg aaggtgaagc aggccgctat
     5041 cgcgcagcag caggcgatca tcgccaagaa acaactacgt gcacccttcg ccggccgcgc
     5101 cggtattgtc accattacgc cggggaccta cctgaattcc ggcacggtga tcgtcacctt
     5161 gcaggagctc gacccggtgt acgtcgattt cttcgtgccg cagaaagacc tggcgcaggc
     5221 tcaggtcggc cgcaatgcca cacttacgct ggatgcgttt ccgggcaagg gatttcgcgg
     5281 cacggtcaat gccgtgaatc cgaaagtcga caccgacacg cgcaatgtac tggtcgaagc
     5341 gcgcgtaccg aatcccgatc gcgtcctcag cccgggcatg ttcgtcaacg tcgcagtcga
     5401 ggtcggctcg cagcagcgtt atctgacgct gccgcagacc gcgatcgtct ataacccgta
     5461 cggcgaaacg gtctacatcg tgcggaccaa gagccaattc gacgcggaac aggccgccgc
     5521 agccgccaag acagccgatc cggtggacaa ggacccgccc aagaagaagg gcaagggacc
     5581 accgccaata cccgccgatg cgctggtggt cgagcaaacc ttcgtgacca caggtcccac
     5641 ccgaggcgac caggtcgcgg tccttagcgg gctcgatgcg ggggtcgagg tggtcagcag
     5701 tggccagatc aagctgaaga atggtgcgcc ggtgcgtatc gacaacagcg tgaagccggc
     5761 cgacaaccca cacccgacgc cacaggagca ttgaagccgg cagtggcgcg gacaaaggat
     5821 ctagagcgca agccatgaat ttcaccgaca tcttcatccg caaacccgtg ctggcgatcg
     5881 tggtcagctc gttgatcttc gtgctggggc tgcgcgcgct caccgggctg ccagtgcgcc
     5941 agtatccgca gacccagaac gctgtggtca cgatcacgac cgcgtactac ggcgcggatg
     6001 cgcagaccat cgccggcttc atcacccagc cgctcgagca ggcggttgcg caggcgcagg
     6061 gcatcgacta tctctcctcc gccagcgcca cagggctctc gaccatcacc gcgacgctgc
     6121 ggctgaacta cgacgccaac cgcgcgctca ccgagatcca gacgcaggtc acctcggtgc
     6181 gcaatcagct gccgcctcag gcgcagcagc cggtgctgaa catcgcgatc gggcagagtc
     6241 tcgacgccat gtacatgggg ttccgcagcg acgtgctgcc ctccaacagc atcaccgatt
     6301 acctcgcgcg cgtggtgaaa cccaagctcg atgcgctgcc gggcgtgcag accgcggaaa
     6361 tcctcggcgg ccggcagttt gcgctgcgtg cctggctcaa tccggcgcag ctggctgcgc
     6421 acggcgttac tgcggcggat gtctttcagg cgctgggtac caacaactat ctggccgcgg
     6481 tcggcacgac caaggggcag atggtgagcg tcgatctgac tgccaacacc gacctgcaca
     6541 ccgtggagga attcaggcag ctcgccatca agcagaaaaa cggcgcgatc gtgcggctgc
     6601 aggacgtggc gacggtcgtg ctcggagcgg aggattacga ctccaacgtc gccttcagcg
     6661 gcaagctggc ggtattcatc ggcgtcaagg ctgcgcccga tgcgaacctg ctcgatgtcg
     6721 cgcagcgggt gcgtaccgcc ctgccggaca tccagtcgca gctgccgcga ggcttgcaag
     6781 ggacgatcgt ttacgacagc accgggttcg tcacctcctc gattcgcgag gtggtgaaga
     6841 ccctcatcga ggcgctgctg atcgtcaccg gcgtgatctt cgtgttcctc ggcagcctgc
     6901 gcgcggtggt cattccggtg atcgccatgc cgctctcgct gatcgggacc ttcttcgtga
     6961 tgctcgcgct cggctattcg atgaacctgc tgacgctcct cgcgctggtg ctcgccatcg
     7021 gcctggtggt cgatgatgcc atcatcgttg tcgagaacgt cgaccggcac atgaaggagg
     7081 aaggcaagag cccgctcgag gcgtcgctgc tcgccgcacg cgagctcggc ggaccgattc
     7141 tcgcgatgac ggtggtgctg gtcgcggtgt acg
//
LOCUS       DATDMP010000266         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1484499, whole genome shotgun sequence.
ACCESSION   DATDMP010000266 DATDMP010000000
VERSION     DATDMP010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484499"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VI195_04525"
     CDS             complement(<1..257)
                     /locus_tag="VI195_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY6823679.1"
                     /translation="MLSDVRAIAFDLDNTLWDVEPVLERAEQRLAAWLQQHCPRMTLS
                     RAEMRAAREQLAEREPHNAHDVSYLRVAALAALARAHGYDER"
     gene            278..928
                     /locus_tag="VI195_04530"
     CDS             278..928
                     /locus_tag="VI195_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6823680.1"
                     /translation="MAPFTLVIGNKNYSSWSLRAWLFMRHVGVEFQEILVRLDAAETR
                     EQLDRYGPSGRVPVLRHGKLCVWDSLAICEYVAELSGVGWPKAREARALARSVSAEMH
                     SGFTTLRSLWPMNARARNRHTAVTAALEADIERIDEIWNDCRGRCGEGGPWLFGDYSV
                     ADAMYAPVVLRFNTYGAHISQTARWYMASVLEDAALQDWLQAAKQEPWTIAADEVG"
     gene            915..>2965
                     /locus_tag="VI195_04535"
     CDS             915..>2965
                     /locus_tag="VI195_04535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY6823681.1"
                     /translation="MKSAEPAESQRMQRLTRPWAPLGLCVCALALPQFRVTQAAENRA
                     RFTVEDLVALKRISDPQVSPDGRLLVFVQRETDLAANKVRSSLWLMALATGARPQQLT
                     DAGGGDASPRWAPDSRTLYFLSTRSGSQQVWRIVLTGAEARRLTDFPLEVGSLRVSPT
                     GSAVAVSMEVFPDCPTLACTRERLDARARTKANGRGYERLFVRHWDRWGDGTRAHLFS
                     VALGAALPTAAAVDVMRGIDADVPGKPFGGDEDFAFSPDGRSLVFAARIAGREESWST
                     NFDLYRVPVEGNAAPVNLTASNPASDAQPVFLANGDLAWIAQERPGFETDRFHIMLRR
                     ARGGSVGALTADWDRSVARLAATSAGRSLLALVDELGQRVLYRIDPRSGARTRLTSVG
                     AVEAISATSDRVVFARADLGGPADLYALPVRGGEPQRLTDVNHDLLGARHLSEYRQFS
                     FRGWNDETVYGYVMKPYGFEPGQRYPIAFVVHGGPQVSMQNLWTYRWNAQAFAGGGYA
                     VVMIDFHGSPGYGQAFTDSISRDWGGKPLVDLQKGLAAAQEQCPWLDGERACALGGSY
                     GGFMMNWIEGNWPDRFRCIVDHDGVFDQRMMYYATEELWFPEWEFGGPYYQNPQGYEQ
                     FNPVDHVAKWRTPILVIHGEQDFRVPYTQGLGSFTAAQRRGIESRLLIFPDENHWVLK
                     AA"
BASE COUNT          463 a          969 c         1029 g          504 t
ORIGIN      
        1 cgctcgtcgt agccgtgcgc gcgcgcgagt gcggcgagcg cggcgacgcg caggtagctc
       61 acgtcgtgcg cattgtgtgg ctcgcgctcg gcgagctgct cgcgtgctgc gcgcatctcc
      121 gcgcgcgaca gcgtcatgcg cggacagtgc tgctgcagcc aggcggcgag gcgctgctcg
      181 gcacgctcca gcaccggttc gacgtcccag agtgtattat cgagatcgaa agcgatggcg
      241 cgcacgtcgc tcagcatcca gctaaggtta gcacctcatg gcgccgttca ctctggtcat
      301 cggcaacaag aattactcct cgtggtcgct gcgagcgtgg ctgttcatga ggcatgtcgg
      361 cgtggagttc caggagattc tcgtccggct cgatgcggcg gaaacgcgcg agcagctcga
      421 tcgctacggc ccgagcggac gcgtgccggt gctgcgacac ggcaagctgt gcgtgtggga
      481 ctcactggcg atctgcgagt atgtcgcgga actgagcggg gtgggctggc cgaaggcgcg
      541 cgaggcgcgc gcgctggcgc ggtcggtgag cgctgagatg cactccggct tcactacctt
      601 gcgctcgctg tggccgatga acgcgcgtgc gcgcaaccgc cataccgcgg tgaccgcggc
      661 gctcgaggcg gacatcgagc gcatcgatga gatctggaat gattgtcgcg gacgctgcgg
      721 tgagggaggg ccgtggctgt tcggtgacta cagtgttgcc gatgcgatgt acgccccggt
      781 ggtgctgcgc ttcaacactt acggcgcgca catctcacaa accgcgcgct ggtacatggc
      841 gagcgtgctc gaggacgccg cgctgcagga ctggctgcag gccgccaagc aggagccctg
      901 gaccatcgcc gccgatgaag tcggctgagc cggcggaaag tcagcgcatg cagcgcctta
      961 cccggccctg ggctccgctc ggcctgtgtg tttgcgcgct cgcgctgcca cagtttcgcg
     1021 ttacacaggc ggccgagaac cgcgcgcgat tcacggtcga ggacctggtg gctctcaagc
     1081 gcatcagcga tccgcaggtg tcgcccgacg ggcgcttgct ggtcttcgtg cagcgcgaga
     1141 ccgacctggc tgccaacaag gtccgtagca gcctgtggct catggcactg gcaaccggcg
     1201 cgcgtccaca gcagctcacc gatgcgggcg gcggcgacgc gagcccgcgc tgggcccccg
     1261 acagtcgcac gctctacttc ctctcgaccc gatccgggag ccaacaggta tggcgcatag
     1321 tgctcaccgg agccgaggca cggcgcctga ccgattttcc cctcgaggtc ggctcgctgc
     1381 gcgtgtcgcc gacgggcagc gcggtcgcag tgtcgatgga ggtgttcccg gattgcccga
     1441 cgctcgcctg cacgcgcgag cgcctcgatg cgcgtgcccg caccaaggcg aacgggcgcg
     1501 gatatgagcg gcttttcgta cggcactggg atcgctgggg cgatggcacg cgcgcgcatc
     1561 tgttcagcgt ggcgctaggc gcggccttac cgacggcggc cgcggtggat gtgatgcgcg
     1621 gcatcgatgc ggacgtcccg ggcaagccct ttggcggcga cgaggatttc gccttcagcc
     1681 ccgacggccg ctcgctggta ttcgcggcac gtatcgcggg gcgcgaggag tcctggtcca
     1741 ccaatttcga cctctaccgc gtgccggtgg agggcaacgc ggcgcccgtc aacctcaccg
     1801 ccagcaatcc ggcgagcgac gcacagccgg tatttctcgc caacggcgat ctcgcgtgga
     1861 ttgcccagga gcggcccggc ttcgaaaccg accgcttcca catcatgctg cgtcgtgcgc
     1921 gcggcggctc ggtcggcgca ctcaccgccg actgggaccg gtcggtcgcg cgactcgctg
     1981 cgacatccgc tggacgctcg ctgctcgcgc tggtagatga gctcgggcaa cgcgtgctct
     2041 atcgcatcga tccccgcagc ggggcgcgaa cgcgactgac ctccgtgggt gcggtggagg
     2101 cgatcagcgc gacctccgac cgcgtggtgt tcgcgcgcgc agatctcggc ggtccggcgg
     2161 atctgtacgc gttgcccgtg cgcggcggcg agccgcagcg actcaccgat gtgaaccacg
     2221 acctgctcgg cgcgcgtcat ctgagcgagt accggcagtt cagcttccgc ggctggaatg
     2281 acgagacagt gtacggatac gtgatgaagc cgtatggctt cgagcccggc caacgttacc
     2341 cgatcgcctt cgtggtacac ggcggtccgc aggtcagcat gcagaatctc tggacctatc
     2401 gctggaacgc acaggcattc gccggcggcg gctacgcggt ggtgatgatc gacttccacg
     2461 gctcgcccgg gtatgggcag gctttcaccg attccatcag ccgtgactgg ggcggcaagc
     2521 cgctcgtcga cctgcagaaa gggctcgccg ccgcgcagga gcagtgtccc tggctcgacg
     2581 gcgagcgcgc ctgcgcgctc ggcggctcct acggcggctt catgatgaac tggatcgagg
     2641 gaaactggcc ggaccgcttc cgctgcatcg tcgaccacga tggggtgttc gatcagcgca
     2701 tgatgtacta cgccaccgag gagctgtggt ttcccgagtg ggagttcggc ggaccctact
     2761 accagaatcc gcagggatac gagcaattca atccggtcga ccacgtcgcc aagtggcgca
     2821 caccgattct cgtgatccac ggtgagcagg attttcgcgt gccctacacc caggggcttg
     2881 gcagcttcac cgctgcgcag cgccgcggga tcgaaagcag actgctgatc ttccccgacg
     2941 agaatcactg ggtgctgaag gctgc
//
LOCUS       DATDMP010000267         2802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1929647, whole genome shotgun sequence.
ACCESSION   DATDMP010000267 DATDMP010000000
VERSION     DATDMP010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929647"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1195)
                     /gene="glcE"
                     /locus_tag="VI195_04540"
     CDS             complement(125..1195)
                     /gene="glcE"
                     /locus_tag="VI195_04540"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY6823682.1"
                     /translation="MGAADQAVPESWRERIAAAVRESMPLRLRGAGTKDFYGEQLSGE
                     ILQLGSWRGIVDYEPSELVISVRCGTPLSEVEAALAAHQQFLAFEPPGFGADPTIGGV
                     IAAGLSGPRRMFAGAARDFVLGTKLLTAQGELLRFGGQVMKNVAGFDVSRLLCGSLGI
                     LGILTEVSLKVLPLPRTEETRRLELPAGEAVAAFNRWAALPLPLSGAAWWQGCAWVRL
                     SGAPAAVRTAREQIGGDPVAAADAEHFWGALRHCQLPPFAAESLWRLSLPQAAAQLDI
                     PIEPLIDWGGALRWYANAPADIDVRARARAAQGTALLWRGLARADRFAPLPPPVASLH
                     RRLKQRFDPAGIFNRGRLIAGL"
     gene            complement(1204..2667)
                     /locus_tag="VI195_04545"
     CDS             complement(1204..2667)
                     /locus_tag="VI195_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6823683.1"
                     /translation="MAGPDATDIAAALRAVLPADCVLERVEERRPYECDGLTAFRAVP
                     AVVVLPRTDEEVRGTLLACHRLKVPLVARGSGTGLSGGATPHPAGVLLSCARMSRILE
                     IDPINRIARVEPGVPNLKISEAAAAHGLYYAPDPSSQVACSIGGNVAENSGGVHCLKY
                     GLTVHNVLGVGGYTVDGERIELGGTAPDNGGLDLLALTIGSEGLLLIVTEVLVKLIPR
                     PPCAQLVMASFADVHSAGNAVAAIIAAGIIPAALEMMDRKATAAVEPFVQAGYDLEAA
                     AILLVESDGTPEEVHEQVQEIERVLRAAGATALRVSASEAERLRLWSGRKNAFPAAGR
                     LSPDYYCMDGTIPRKHVGHVLEAIERMEKRYALRCMNVFHAGDGNLHPVILFDANEPG
                     CWDRADRFGAEILELCVTLGGTITGEHGVGLEKLNSMCVQFSPAERAAFFAVKEAFDP
                     DGLLNPGKLIPTLVRCAEYGRMHVHGGALPHPHLPRF"
BASE COUNT          504 a          961 c          938 g          399 t
ORIGIN      
        1 cggcactgcg gctgcgcatc gacgcggagc ggcaggtgct cgtgagcgga gatgtcatcg
       61 agctcggacg gggcacgatc aatctctagc gcctcgcggc gagcgcatcg ctcgccagcg
      121 ctagtcacag cccggcaatc aacctgccgc ggttaaagat acctgccgga tcgaagcgtt
      181 gcttcagacg ccgatgcagg ctcgccaccg gcggcggcaa tggcgcgaag cggtccgcgc
      241 gcgcgaggcc gcgccagagg agcgccgtac cctgtgccgc ccgcgcacgc gcacgtacgt
      301 caatgtccgc tggggcattc gcgtaccagc gcagcgcccc tccccagtcg atgagaggct
      361 cgatcgggat atcgagctga gccgccgcct gaggtagcga cagccgccac agactctccg
      421 ccgcaaaggg cggcaactga caatgccgca gtgcgcccca gaagtgctcg gcatccgctg
      481 cggctaccgg atcgccaccg atctgctccc gcgcggtgcg gacggcggcg ggtgcaccgg
      541 acaagcgcac ccaggcgcac ccctgccacc aggccgcacc cgaaagcggc aggggcagag
      601 ccgcccagcg attgaaggcc gccaccgctt cgccggcagg cagctcgaga cgccgcgttt
      661 cttccgtgcg cggcagcggc agcactttga gcgagacttc ggtaaggatt ccgaggatgc
      721 cgagtgagcc gcagagtagc cgcgagacat caaaccccgc gacattcttc atcacctgtc
      781 cgccgaagcg cagcagctcg ccctgcgccg tgagcagctt cgtgccgagc acgaagtcgc
      841 gcgcagcgcc ggcgaacatg cgccgtggtc ccgaaagccc cgcggcgatc actccaccga
      901 tggtcgggtc ggcgccgaaa cccggcggct cgaaggcgag gaactgctgg tgcgcggcga
      961 gggccgcctc gacttccgac agcggcgtcc cgcagcgcac actgatgacg agctcggaag
     1021 gctcgtagtc gacgatcccg cgccagctgc cgagctgcag gatctcaccg ctgagctgct
     1081 cgccgtagaa gtccttggtc ccagcgcccc gcaggcgcag tggcatcgac tcacgtacgg
     1141 cggcggcgat gcgctcgcgc caactctcgg gaaccgcctg gtccgccgcg cccatgcgag
     1201 cgctcagaag cgcggcaggt gcgggtgagg cagggcaccg ccgtggacgt gcatgcgtcc
     1261 atattcggcg cagcgcacca gtgttggaat gagcttgccg ggattgagca agccatccgg
     1321 gtcgaaggct tccttgacgg cgaagaaggc ggcacgctcg gcaggggaga actgcacgca
     1381 catggagttg agcttctcga gtccgacacc gtgctcgccg gtgatggtgc cacccagtgt
     1441 cacgcacagc tcgaggatct cggcaccgaa gcgatcggcg cggtcccagc agccaggctc
     1501 gttggcatcg aacaggatca ccggatgcag gttgccgtcg cccgcgtgaa agacattcat
     1561 gcagcgtagc gcgtaacgct tctccatccg ctcgatcgcc tcgagcacat gacccacgtg
     1621 cttgcgcgga atggttccat ccatgcagta atagtcgggc gacagacggc ctgctgcggg
     1681 aaaagcattc ttgcggcccg accacaggcg tagccgctcc gcttcggagg ccgacacgcg
     1741 caacgcggta gcacccgctg cacgcagcac gcgctcgatc tcctgcacct gctcgtgcac
     1801 ctcctccggg gtgccatccg actccaccag caggatggcg gccgcctcga ggtcgtagcc
     1861 cgcctgcacg aacggctcga ccgcagcggt cgccttgcgg tccatcatct cgagcgcggc
     1921 cgggatgatg ccggcagcga tgatggctgc tacggcgttg ccggcgctgt gcacgtcggc
     1981 gaaggaggcc atgacgagct gcgcgcacgg cggccgcgga atgagcttca cgagcacctc
     2041 ggtcacgatc agcagcaatc cctccgagcc gatcgtgagc gcgagcagat cgagaccccc
     2101 gttgtcgggg gcagtgcccc cgagctcgat gcgctcccca tccaccgtgt agccgccgac
     2161 gccgagcacg ttgtgcaccg tgagtccata tttcaggcaa tgcacgccgc cggaattctc
     2221 agccacattg ccgccgatcg aacaggccac ctgcgaggat ggatctggcg cgtagtagag
     2281 tccgtgcgcg gcggcagctt cggagatctt caggttggga actccaggct cgacacgcgc
     2341 gatgcgattt atgggatcga tctcgagtat ccggctcatg cgcgcacagg agagcagcac
     2401 cccggcaggg tggggcgtgg cgccgccgga gagcccggtg cccgagccac gtgcaaccag
     2461 cggcaccttc agacgatggc aggcgagcag cgtaccgcgc acttcctcat cggtgcgcgg
     2521 cagcacgacc acagccggca cggcgcggaa cgcggtgagc ccatcgcatt cgtagggccg
     2581 gcgctcctcg acacgctcca gcacgcagtc cgcggggagc acggcacgca gcgcggcggc
     2641 aatgtcagtg gcgtcgggtc cggccatggg ggccatcgta atggcgctgc cggagaagtc
     2701 catgccgcaa atcgatcagt tcacgcgggg ggtgctgatg ataatgggcc tcacgcccgg
     2761 caggatgagt tcatggatca gcagccaacg agccaaccgg ca
//
LOCUS       DATDMP010000268         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1708081, whole genome shotgun sequence.
ACCESSION   DATDMP010000268 DATDMP010000000
VERSION     DATDMP010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708081"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VI195_04550"
     CDS             complement(<1..300)
                     /locus_tag="VI195_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY6823684.1"
                     /translation="MQGKHILLGVTGGIAAYKSPDLVRRLRERGAEVQIVMTRGAREF
                     LTPTTFQAVSGRAVRSELWDAAAEAAMGHIELARWADAVLIAPASADFLARLAVGL"
     gene            463..1137
                     /gene="radC"
                     /locus_tag="VI195_04555"
     CDS             463..1137
                     /gene="radC"
                     /locus_tag="VI195_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY6823685.1"
                     /translation="MTIRHWPAAERPREKLLERGAHALSDAELLALLLGSGTRSHSAV
                     ELARGLIAQFGSLRMLLNAEAERCLAQAGIGPARYAILKAALELARRHFREALHLGPA
                     LAAPEATRTFLVAQLRDRPYEVFCCLFLDNRHRLIAFEELFRGTIDRAGVHPREVLRQ
                     TLLHNAAALIFAHNHPSGVLEPSQADELITRRLKEALALVDVRVLDHFIIGDGLCFSF
                     SEHGLI"
     gene            1591..1827
                     /gene="rpmB"
                     /locus_tag="VI195_04560"
     CDS             1591..1827
                     /gene="rpmB"
                     /locus_tag="VI195_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY6823686.1"
                     /translation="MSRVCEVTGKKPGVGNRVSHANNKKRRRFLPNLHTQRFWLETER
                     RWVTLRLTTSGMRTVEKKGIDKVVAELRAQGHKF"
     gene            1850..2005
                     /gene="rpmG"
                     /locus_tag="VI195_04565"
     CDS             1850..2005
                     /gene="rpmG"
                     /locus_tag="VI195_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY6823687.1"
                     /translation="MREKIKLLSSAGTGHFYVTTKNKRLHPDKLEVRKFDPVARKHVA
                     YKESKIK"
BASE COUNT          361 a          726 c          697 g          365 t
ORIGIN      
        1 caatcccacc gcgagccgcg cgagaaagtc cgcgcttgcc ggcgcgatga gcaccgcatc
       61 cgcccagcgg gcgagctcta tgtgccccat ggcggcctcg gccgctgcgt cccacagctc
      121 gctgcgcacg gcgcgccccg agacggcctg gaaagtggtc ggggtgagga attcgcgcgc
      181 gccccgagtc atgacgatct gcacctcggc gccgcgctcg cgcagccggc gcaccaggtc
      241 cgggctcttg taggcggcga tcccgccggt gaccccgaga agaatgtgct tgccttgcat
      301 ggatctcgtc tcgttcccgc gattatcgca ccctcccccg ccaggcgcgt aagcacgcgt
      361 gcgcagagtg gggaatagcc ccattcggag ggctttctcc ccctcgcgct cggcgggcat
      421 gccggcttag attcggcgcg catctggtta gaacccacgg taatgaccat ccgccattgg
      481 cccgccgccg agcgcccgcg cgagaaatta ctcgagcgcg gcgcacacgc gctttccgat
      541 gcagagctgc tcgcgctgct gctcggctcg ggcacccgca gccactccgc ggtcgagctg
      601 gcacgcgggc tgattgcaca gttcggctcg ctgcgcatgc tgctgaacgc cgaggcggaa
      661 cgctgcctgg cgcaggcggg aatcggaccg gcacgttatg ccatcctcaa ggcggcgctg
      721 gagctggcgc gacgccactt ccgggaagct ctgcatctgg gtccggcact cgcagcgccc
      781 gaggcgaccc gcacctttct cgtggcacag ctgcgcgatc ggccgtacga ggttttctgc
      841 tgcctgttcc tcgacaatcg ccaccggctg attgccttcg aagagctgtt tcgcggaacc
      901 atcgatcgcg ccggcgtgca cccgcgcgag gtgctgcggc agactctgct gcacaacgca
      961 gcggcgctga tcttcgctca taaccatccc tcgggcgtgc tcgagcccag ccaggcggat
     1021 gagctgatca ctcgacggct gaaggaggcg ctggcgctgg tcgatgtgcg ggtgctcgac
     1081 catttcatca tcggcgatgg gctgtgcttc tcgttttcgg agcacgggct catctagaac
     1141 gagcggcggg cgcttacttg aaaatcgctt cctgcgaagt gcgcgcgccg agcacggtgc
     1201 ggatcttggc gcggtccgcc tccgacttgt cggccgcctc cacggagccc tcgatcttcg
     1261 cgctcacctg gtgatgagcc cagcggcctt ggcggcctcc tgctgcttct tgtaggtggt
     1321 cgccagccaa tgcatgtaat cgttgaaatg accatcaacg tggtgacggc ggcaagagca
     1381 ggcagcgccg cgatggcgca gagacatcga gtcaaacgca tgggaccctg cccggggacc
     1441 aagcgtcgcc agaacgcctc cgacattcct ccgcgcatgg agcgcgtggc ggcttcttgt
     1501 gatcgctccc cggtgctggt ataaagcccg gcccgggtcg ggtgggctgt acactagatc
     1561 ctaagtcgct gaatctcaag gttgttctat atgtcccgcg tctgcgaagt caccggcaag
     1621 aagccggggg tcggaaatcg cgtctcgcat gccaacaaca agaagcgccg gcgcttcctg
     1681 ccaaatctgc atacgcagcg tttctggctg gagaccgagc ggcgctgggt gacgctgcgc
     1741 ctcacgacca gcggcatgcg taccgtcgag aaaaagggca tcgacaaggt cgtcgctgag
     1801 ctgcgcgccc agggccacaa gttctgacgc gcgctccgaa ggagtcatca tgcgcgagaa
     1861 gattaagctg ctgtcctcgg ccggaaccgg ccacttctac gtgacgacca agaacaagcg
     1921 cctgcatccg gacaagctgg aagtgcgcaa gttcgacccc gtggcgcgca agcacgtcgc
     1981 ctacaaagag tccaagatca agtaactccg ctgcccgagc tgcccgaagt cgagacgaca
     2041 cggcgcggcc tggcgccgca cgcgcggcgc cggcgcatcg cgcgcctcag ggtgtatgag
     2101 ccgcggctgc gctggcgcgt ggcacgcgat ctgccgcgca aggtcgccg
//
LOCUS       DATDMP010000269         2811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1596593, whole genome shotgun sequence.
ACCESSION   DATDMP010000269 DATDMP010000000
VERSION     DATDMP010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596593"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VI195_04570"
     CDS             complement(<1..540)
                     /locus_tag="VI195_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6823688.1"
                     /translation="MNDNPMPSAQAPITRSHVLKAENLCKQVSSPEGSLTIVDDVSLA
                     IGAGESVAVSGPSGAGKSTLLALLAGLDLPTRGRVLLKGQDLTRLDEDGRARLRAQRV
                     GFVFQSFHLIPALTALENVMLPLELAGGSDARRTAAETLAGVGLARRTGHYPRQLSGG
                     EQQRVAIARAFVTRPAVLFA"
     gene            557..1138
                     /locus_tag="VI195_04575"
     CDS             557..1138
                     /locus_tag="VI195_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY6823689.1"
                     /translation="MASASTVLVFGDSLSAAYGIRPEQGWVTLLTQRLKAQGYGYQVV
                     NASVSGETSSGGLERLPRALNLHTPGIVVLELGANDGLRGLPVSALRDNLAQMVRLAQ
                     AAGAHVLLVGIRIPPNYGPRYTDEFARVYGELAQQYHLPLVPFLLEKVALDPALMQED
                     GMHPNAAAEPIVLDTLWPYLKPLLNDKAHLGGR"
     gene            1138..>2811
                     /locus_tag="VI195_04580"
     CDS             1138..>2811
                     /locus_tag="VI195_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6823690.1"
                     /translation="MDKIWLKSYPPGVPAQIDPSRLRSLNELVDRTCAEHAERVAYVQ
                     MDAALTYRQVDQMSRAFAAWLQQTGFAKGDRFAIMLPNTLQYPIALFGALRAGLIVVN
                     TNPLYTAPELEHQLSDSGATGILVLENFAHVLEKVLPRTSIKHVLVTAVGDLLGFPKS
                     TIVNFVVRHVRKQVAPWNIPQAASFKAVVAQGKKLAFAPVEVAPADLAFLQYTGGTTG
                     VSKGAMLSHGNVATNVLQAEAWLGTRLRDFSNGGVVITAIPLYHIFALQANCFLFARL
                     GWNNVLIINPRDLPAFIQELTKHPPNYITGVNTLFNALLHAPGFAQIDFSHLRAALGG
                     GMAVQAAVAQRWKEATGQVLTQAWGLTETSPAACINPPDLDFNGSIGLPISSTEIAIK
                     DDAGNDLELGQIGEICVRGPQVMRGYWKRPDETAKVMLPGGWLRTGDVGHMDAQGFVY
                     IEDRKKDMILVSGFNVYPNEVEAVAANCPGILEAAAVAQRDEYSGEVVALFAVRKDPA
                     LTEHDVVECCRKSLAGYKVPKHVYFRNELPKSNVGKILRKALRQELSGPS"
BASE COUNT          526 a          897 c          911 g          477 t
ORIGIN      
        1 cgcgaacagc accgccgggc gcgtgacgaa ggcgcgggcg atggccacgc gctgctgttc
       61 gccgccggag agctgccgtg gatagtgccc ggtgcgccgc gcgagcccca cccccgccag
      121 ggtctcggcc gccgtgcggc gcgcatcgct accgccggcc aactcgagcg gcagcatcac
      181 attctcgagc gcggtcagcg ccggaatgag gtggaaggac tggaacacga agcccacccg
      241 ttgggcacgc aggcgtgcgc ggccatcctc gtcgagacgc gtgagatcct ggcccttgag
      301 cagcacgcgg ccacgcgtcg gcagatcgag tccggccaac aatgcgagca gtgtcgactt
      361 gcccgcgccc gatggaccgc tcaccgccac cgactcgccg gcgccgatgg cgagcgagac
      421 atcatcgaca atggtcagcg aaccttcggg gctgctaacc tgcttgcaga ggttttcagc
      481 cttgagaacg tggctgcggg tgatcggcgc ctgggcgctg ggcatggggt tgtcgttcat
      541 cgcgctccag aacgcggtgg cctccgccag tacggtcctt gtatttggcg acagcctgag
      601 cgccgcctac ggaatccggc cggaacaggg ttgggtaacg ctgctcacgc agcgactcaa
      661 ggcacaaggg tacgggtacc aggttgtcaa cgccagtgtg agcggcgaga ccagcagcgg
      721 cggcctcgag cggctgccgc gtgcgctcaa cctgcacacc ccgggcatcg tagtgctcga
      781 gctgggtgcc aacgatggtc tgcggggttt gccggtgagc gcgctgcgcg acaaccttgc
      841 ccagatggtg cggctcgcgc aggccgccgg cgcgcacgtg ctgttggtcg gtattcgcat
      901 tccacccaac tatgggccgc gctacaccga tgagttcgcg cgcgtttatg gcgagctcgc
      961 gcagcaatac catctgccgc tggtgccgtt tctgttggag aaggtggcgc tcgatccggc
     1021 tctcatgcag gaggacggca tgcatcccaa tgctgccgcc gagccgatag tcctcgatac
     1081 actgtggcca tatctcaaac cattgctcaa cgacaaagcg cacctcgggg ggcgttgatg
     1141 gacaaaatct ggctgaaatc gtatccaccg ggagtaccgg cgcagatcga tccgagccgg
     1201 ctgcgctcgt tgaatgagct ggtggacagg acctgcgccg aacatgccga acgggtcgcc
     1261 tatgtgcaga tggatgcggc gctcacctac cggcaggtcg accagatgag ccgcgctttc
     1321 gccgcctggc tgcagcagac cgggttcgcc aaaggcgacc gcttcgccat catgctgccg
     1381 aacacactgc agtaccccat agcgctgttc ggcgcactgc gcgccggcct catcgtcgtc
     1441 aacaccaacc cgctgtacac ggcaccggaa ctggagcacc agctcagcga ctccggcgcc
     1501 actgggatcc tcgtgctcga gaacttcgcg cacgtgctgg aaaaagtgct gccgcgaacg
     1561 agcatcaagc acgtgctggt gaccgcggtc ggagacctgc tcggcttccc caagtccacg
     1621 atcgtgaact tcgtagtgcg tcacgtacgc aagcaggtag ccccctggaa cattccgcaa
     1681 gcggcgtcgt tcaaggcggt ggtggcgcag ggcaagaagc tggcgttcgc accggtggag
     1741 gttgccccag ccgacctcgc cttcctgcag tacaccggcg gtaccaccgg cgtatcgaaa
     1801 ggcgccatgc tttcgcacgg caacgtggca accaacgtgc tgcaggccga agcgtggctc
     1861 ggtacgcgcc tgcgggattt cagcaatggc ggcgtggtga ttaccgctat tccgctctac
     1921 cacatcttcg ccctgcaggc gaactgcttt ctgttcgcac ggctcggctg gaacaacgtg
     1981 ctgatcatca atccacgcga cctgcccgcc ttcatccaag agcttacaaa gcatccaccc
     2041 aactacatca ccggtgtcaa cacgctcttc aacgctctgc tacatgcgcc ggggtttgcg
     2101 caaatcgatt tcagtcatct gcgggccgca ctcggtggcg gaatggccgt ccaggcggcc
     2161 gtggcgcaac gctggaagga ggccaccggc caggtgttga cccaggcctg gggcctcacc
     2221 gagacatcgc ccgccgcgtg catcaatcct ccggatctcg acttcaacgg ctcgatcgga
     2281 ctgcccatct cctcgaccga gatagcgatc aaggacgatg ccggcaacga cctcgagttg
     2341 ggtcagatcg gcgagatttg cgtccgcgga ccgcaggtca tgcgcggcta ctggaagcgg
     2401 ccagacgaga ccgcgaaagt gatgttaccc ggcggctggc tgcgcaccgg agacgtcggt
     2461 cacatggacg cgcagggatt cgtgtacatc gaggaccgca agaaggacat gatcctggtg
     2521 tccggattca atgtgtatcc caacgaggtg gaagcggtcg cggccaactg tccggggatt
     2581 ctcgaggccg cagcggtggc gcagcgcgac gagtattcgg gggaggtcgt ggcgttgttc
     2641 gcggtgcgca aggatccggc gctcaccgaa cacgatgtgg tcgagtgctg ccggaagtca
     2701 ctcgccggtt acaaggtccc gaagcatgtg tacttccgga atgagctgcc gaaaagcaac
     2761 gtcgggaaga ttttgagaaa ggcgttgagg caggaactaa gcggcccgag c
//
LOCUS       DATDMP010000270         2219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1041086, whole genome shotgun sequence.
ACCESSION   DATDMP010000270 DATDMP010000000
VERSION     DATDMP010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041086"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VI195_04585"
     CDS             complement(<1..776)
                     /locus_tag="VI195_04585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY6823691.1"
                     /translation="MDERANATLTLLPSEDEVLVSDPDAAHARSSELASQLRAAAMKG
                     DKVRVTRLLSQLLGFRGVTAQRRTALDELSGLIDSLRSLAMTDELTGIYNRRGFMHIA
                     ARFLDVARRDLQCAYLVYLDLNNLKQVNDAAGHATGDVLIRQTASFLRELFPSYGVYE
                     ILGRLGGDEFAALTTDMKCPSRSEILLRARLFQGRSCTLPPLSLSIGFAYFNPLLPLG
                     ISELLQSAERAMYEHKRTRRSPPRPAVHGAAADRPRSRSP"
     gene            1056..1805
                     /locus_tag="VI195_04590"
     CDS             1056..1805
                     /locus_tag="VI195_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6823692.1"
                     /translation="MRGSGAGGCEAVKSPTPKSIRILLVDDHAILREGVRALLEAEAD
                     IEVVGEAGTPAEATRLAREAQPTLAIVDLMMACESGLKTIGELRGACAALRALVLSAH
                     FSDEYLSAALAAGADGYVLKSAKAVDLLRAIRSVAAGHRFFSSEVSGKLVSAYLRRDE
                     RATDGHTRITPRERQLLVRIALGDSNKRLAAMMCRSVKTVEKHRANLMRKLRLRNTAE
                     LTLFAVRSGVVPLSGGAGQWTDGGPSVPAGA"
     gene            1810..>2219
                     /locus_tag="VI195_04595"
     CDS             1810..>2219
                     /locus_tag="VI195_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY6823693.1"
                     /translation="METRAPLLRPPRVLLIGPYDPNCGEYTFLAPPLGVWRLAGVLES
                     AGAEARVFDPNCCPGPPGQALERELRSSVWDVIGVSTTGMTLRFDLQLAHLARRLAPH
                     ALLVAGGMEATFRPDLMFRLGPFDRVVLGEGERPL"
BASE COUNT          330 a          735 c          790 g          364 t
ORIGIN      
        1 tctggcgaac gggaccgggg gcgatccgct gcggcgccgt gcactgccgg gcgcgggggg
       61 ctgcggcggg tgcgcttgtg ctcgtacatg gcacgctcgg cactttgcag cagctcgctg
      121 attccgaggg gcagcagggg attgaagtac gcgaacccga tgctgagtga cagcggcggc
      181 agcgtacacg agcgcccctg gaacaggcgg gcgcgcagca gaatctcgct gcgcgagggg
      241 catttcatgt cggtggtgag agcggcgaac tcgtcaccgc ccagccggcc aaggatctcg
      301 tacaccccgt agctcggaaa cagctcgcga agaaagctgg cggtctggcg gatgagcacg
      361 tccccggtgg cgtgtccggc agcgtcgttc acctgcttga gattgttgag atcgaggtag
      421 acgaggtacg cgcactgcag atcgcgccgt gcgacgtcga ggaagcgggc ggcgatgtgc
      481 atgaatcccc gccggttgta aatgccggtg agctcgtcgg tcatggcgag gctgcgcagc
      541 gagtcgatca gcccgctgag ctcatcgagc gcggtccggc gttgcgccgt gactcctcgg
      601 aaaccgagca gctgcgacag cagccgggtg acgcgcacct tgtcgccttt catggcggcg
      661 gcgcgcagct gactcgcgag ctcgctgctg cgggcgtgcg ctgcgtccgg gtcgctcacc
      721 agtacctcgt cctcgctagg cagcagtgtc agtgtcgcgt tcgcccgttc gtccatgatc
      781 ggctcctctc cgggtcgtcg gcggccggag tgagcgcagc ataagagcgg cggccggcgc
      841 tgcaccacag caggattccc cgcttgccgc tgcggaaatc acgcgtccgg cgctagggga
      901 tctccggatg acaccgcatc cggagattcc tgatgaacgg ctgcgcctct caccggactc
      961 gcggctgcgg catccgccgg gttcgggagc gttcacgggc ctctagactc gcttcacttt
     1021 agccaaccga ctggaagctc taaggagcgc gacccatgag agggagcggc gcaggcgggt
     1081 gtgaagcggt gaaatctccg actccgaagt ccatccgcat cctgctggtc gacgatcacg
     1141 cgatcctgcg cgaaggcgtg cgcgcgttgc tcgaggccga agcggacatc gaggtggtcg
     1201 gtgaagcagg cacccccgcc gaagccaccc gcctggcacg cgaggcgcaa ccgacgctcg
     1261 cgatcgtcga cctgatgatg gcgtgcgaat cgggtctgaa gaccattggc gagctgcgcg
     1321 gcgcctgtgc tgccctgcgg gcgctggtgc tctcggcgca cttctcggac gagtacctca
     1381 gtgcggcgct cgcggcgggc gccgacggct atgtgttgaa gagcgcgaag gcggtagatc
     1441 tgctgcgtgc gatccgctcg gtcgccgccg gtcacaggtt cttctcgagc gaggtgtccg
     1501 gcaagctggt gtccgcctac ctgcgccgcg acgagcgcgc gaccgacggc cacacgcgca
     1561 ttacgccacg cgagcgccag ctgctcgtcc gcatcgcgct cggcgactcg aacaagcggc
     1621 tggccgccat gatgtgccgc agcgtcaaga cggtggagaa gcaccgcgcc aacctgatgc
     1681 gcaagctcag gctgcgcaac acggccgagc tcactctgtt cgcggtgcgc agcggcgtcg
     1741 taccgctgag cggcggcgcc gggcagtgga cggacggcgg gccgtcggtg ccggccggcg
     1801 cgtgagccca tggaaacgcg cgctccgctg ctgcgtccgc cccgggtcct gctgatcggt
     1861 ccctacgacc cgaactgcgg cgagtacacc tttctcgcgc ccccgctcgg ggtctggcgt
     1921 ctcgccggcg tgctcgaatc cgccggggcc gaggcgcggg ttttcgatcc caattgctgt
     1981 cccggacctc ccgggcaggc cctggagcgc gaactgcgca gcagtgtctg ggacgtgatc
     2041 ggcgtctcga ccacggggat gacgctgcgg ttcgatctgc agctcgccca tctcgcgcgg
     2101 cgcctcgccc cgcacgcact gctggtcgcg ggcggcatgg aggctacttt caggcccgat
     2161 ctcatgttcc gcctcgggcc cttcgatcgc gtggtgctcg gggaaggcga gcgtccgct
//
LOCUS       DATDMP010000271         8020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1708439, whole genome shotgun sequence.
ACCESSION   DATDMP010000271 DATDMP010000000
VERSION     DATDMP010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708439"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VI195_04600"
     CDS             complement(<1..1286)
                     /locus_tag="VI195_04600"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY6823694.1"
                     /translation="MSDRTASTATPFPSNIEIAQRARLRPIIPLARERLGIPEESLEP
                     YGHYKAKVSLDYLAQLADRPDGKLVLVTAISPTPAGEGKTTTTVGLGDALNLLGKRAI
                     MCLREPALGPVFGMKGGAAGGGYSQVVPMEDINLHFTGDFAAVALANNLLAALIDNHI
                     YQGNALGFDVRRVAWRRVVDINDRALREIVIGLGGTANGVPREDGFDIVVASEVMAIL
                     CLASSLGDLKERLGRIVVGYRAGGEPILARDLKAHGAMTALLKSALAPNLVQTLANNP
                     AFVHGGPFANIAHGCNSVMATRTALKLADVVVTEAGFGADLGAEKFVDIKCRKSGLRP
                     SAVVIVATLRALKYHGGADLKELNQENLPALEKGLANLERHVHNVRNHYGLPCVVSIN
                     QFTFDTPAELALIQKKMAHHDVPVILARHWAEGGKGA"
     gene            1343..1759
                     /locus_tag="VI195_04605"
     CDS             1343..1759
                     /locus_tag="VI195_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY6823695.1"
                     /translation="MLWLKALHVVFVVAWFAGLFYLPRLYVYHAVTSDAPGLARFVVM
                     ERRLFLLMTIGALLAVLFGLAMILASPGYLAQGWLRLKLLLVALLVGYHFWCYRLMLA
                     LRLGANPHSQRWYRWFNELPGLFLIAIVILAVVKPW"
     gene            complement(1865..3961)
                     /locus_tag="VI195_04610"
     CDS             complement(1865..3961)
                     /locus_tag="VI195_04610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY6823696.1"
                     /translation="MAALLALVGCAGKPVEEKSVLPNTQAAAPAAGAAAATSARAGEA
                     APRTSVAIGDFGLDLSALDPTVRPGDDFFAYANGGWYQRFTIPADKSSFGPFDRLDEL
                     SKERVRAIIEKAAAAHAPAGTPEQQIGDYYAAYMDQAAIETNGLAPAQGDLQRIAALR
                     TREEVAHLFGEPGFATLFDVQLPPDFKNPDRYSVFISESTLGLPDRDYYLKDDPALKE
                     LRAKYVAYIEQMLTLGGTVDAAAKARGIMAFETEASKVQWPLEKRRDVDAIYNPRSKA
                     QLLAYAPGFPWQPFLAAQQLGAREQVVLGELTAIRGLAAVFGRTDVATLRDFLTFHYL
                     TSHAPYLPQRFDEARFAFYGQTMRGQPQQRERWKRAVDAVDDSVGEAVGQLYVTRYFP
                     PESKAKMQQLVAGLEAALSERIDALDWMTPQTKARAHEKLAAFTPKIGYPDKWKDYSK
                     LIVRRDDLLGNVRRAAEWQWNYQVERLDKPVDRSEWQMTPQEINAYYNPSNNEIVFPA
                     AILQPPFFDPNADAAVNFGAIGAVIGHEMGHGFDDQGRKFGPDGAMRDWWTAADAQVF
                     TARTARLIKQFSDFEALPGLKVNGANTIGENIGDLGGLNMAHEAYRISLKGQPAPVID
                     GLSGDQRFFLAYAQIWREKYREGALREMVMSNEYSPAKFRVNGPLPNIDDWYSAFNVQ
                     PGDKLYMKPADRVRIW"
     gene            complement(4074..4670)
                     /locus_tag="VI195_04615"
     CDS             complement(4074..4670)
                     /locus_tag="VI195_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY6823697.1"
                     /translation="MLILPAVCDNPLMQAIDALLTRRSARALIEPAPDNGALELIFSA
                     AARAPDHGRLRPWRFVVVRGAARERFGELLAEQLRRTRPQSAQEALQRERLKAFRAPL
                     IIVVAAHCEPAVKIPVIEQTLSAGAAAHAMMLAAFALGFNAMWKTGAAAYDAAVKQAL
                     GLEAADAIVGFLYFGTESGERAPAARTEWRDRVRELPA"
     gene            4701..5291
                     /locus_tag="VI195_04620"
     CDS             4701..5291
                     /locus_tag="VI195_04620"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY6823698.1"
                     /translation="MLTQSEACERNKKPILAVLREELVACTRVLEVGSGTGQHAVYFA
                     AALAQLIWQPSELAENLPSLEERIRLEGGANLLAPVALDVVADPWPVAPVDAVFSANT
                     LHIMGWSAVCEFFRGAAEVLARPGALCVYGPFRFAGRHTSESNVAFDQWLKQRDPASG
                     VRDFEALDRLAQQRGLALTANHAMPANNHTLVWRKA"
     gene            5302..5847
                     /locus_tag="VI195_04625"
     CDS             5302..5847
                     /locus_tag="VI195_04625"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY6823699.1"
                     /translation="MDPDALRAIREAIRTIPDYPKPGVMFRDITTLLGNAHAFHQVVE
                     ELARPWRNTQVDHVAGIEARGFILGGAIAHQLHAGFVPIRKKGKLPHTTVRVAYSLEY
                     GLDEMEMHRDAVSPGERIVLVDDLIATGGTAEAAVRLLHSLGASILAACFIIDLPDLG
                     GSTRLAALDVPVRTLVAFHGH"
     gene            6008..6217
                     /locus_tag="VI195_04630"
     CDS             6008..6217
                     /locus_tag="VI195_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823700.1"
                     /translation="MFKKLVPVIALALLAGPVFAADAPASTPAADQSSTASSKHHKKS
                     HSSSHSSSHHKKSTTTDASSTAPAK"
     gene            6311..6598
                     /locus_tag="VI195_04635"
     CDS             6311..6598
                     /locus_tag="VI195_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823701.1"
                     /translation="MDGTQQAAIHQALVSVQLAVTSMTFPSCDQDDLIEAIDRVEEQL
                     HASHPNLALVCRFLNSIARSLRAQPEARDACLLIEDAIGKAGVPSTWQSGI"
     gene            6840..>8020
                     /locus_tag="VI195_04640"
     CDS             6840..>8020
                     /locus_tag="VI195_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6823702.1"
                     /translation="MFTPIAQSLILLAGSVFLITLVRRLSLPTSLAYLLVGLVLGPHA
                     LGVVSDSGTTRLLAELGVAFLLFTLGLEFSLPRMLAMRREVFGLGALQVVATTAVFAL
                     IGHLLGIRWLLAVVLGGAVAMSSTAMLLQQLTERAELNRTHGRLAFAMLLFQDLAFVP
                     FFALGALLSAGRERFAVSESLEALGSAVLALIGVLAAGRWLLRPLFHEISHSRLRELF
                     TLTVLLVVLSSAWVSHLSGLSFALGAFLAGMMLGETEYRYQIDTAIRPFRDLLLGLFF
                     VSVGMLLNLHLLWHPSELAIALAMLVTLVVLKAVLAALATWPFTHSRFKALRTGIVMS
                     IGGEFGIALCTVLLQARLVPEGLVEPLLVAIVLSMLLSPFILNNNKRVARALLRERAP
                     EP"
BASE COUNT         1233 a         2663 c         2647 g         1477 t
ORIGIN      
        1 gcgcccttgc cgccctccgc ccaatggcgc gcaaggatga cgggtacgtc atggtgcgcc
       61 atcttcttct ggatcaatgc cagctcggcc ggcgtgtcga acgtgaactg gttgatcgac
      121 accacgcacg gaaggccgta atggttgcgg acgttgtgca cgtgccgttc gagattggcc
      181 aaccccttct ccaacgccgg caggttctcc tggttgagct ccttcaagtc cgcgccgccg
      241 tgatacttga gtgcgcgcaa cgtggccacg atcacgaccg ccgagggccg caggcccgac
      301 ttgcggcact tgatgtcgac gaacttctcg gcgcccaggt cggcgccgaa gccggcctcg
      361 gtcaccacca cgtcggccag cttcagcgcg gtgcgcgtcg ccatcaccga attgcagcca
      421 tgcgcgatgt tggcgaacgg accgccgtgc acgaaggccg ggttgttggc cagtgtctgc
      481 accagattcg gcgccagtgc cgacttcagc agcgcggtca tggcgccatg cgccttgaga
      541 tcgcgcgcga ggatcggctc gccgcccgca cggtagccga ccacgatccg cccgagacgc
      601 tccttgaggt cgccgagtga gctcgccaga cacagtatgg ccatgacctc cgatgccacg
      661 acgatgtcga aaccgtcctc gcgcggcact ccgttggcag tgccgccgag tccgatgacg
      721 atctcgcgca gcgcgcggtc gttgatgtcg accacgcgcc gccaggcgac gcggcgcacg
      781 tcgaagccga gcgcgttgcc ctggtagatg tggttgtcga tgagcgcggc cagcaggttg
      841 ttggcgagcg ccacggcggc aaaatcgccg gtgaagtgca ggttgatgtc ctccatcggc
      901 acgacctgcg agtaaccacc gccggcggca ccgcccttca tgccgaacac gggcccgagc
      961 gccggctcgc gcaggcacat gatcgcgcgc ttgccgagga ggttcagggc atcgccgagc
     1021 ccgacggttg tcgtggtctt gccctcgcct gccggcgtcg gcgagatcgc ggtaaccagc
     1081 accagcttgc catcgggccg gtccgcgagc tgcgcgagat aatcgagcga taccttcgcc
     1141 ttgtaatgtc catagggctc gaggctttcc tcggggatac ccagccgttc ccgcgccagc
     1201 gggatgatcg gacgcagccg cgcgcgctgc gcgatctcga tgttgctcgg aaagggcgta
     1261 gccgtagagg ccgtgcggtc agacatggtc gggtggggtg ccgtggtgga aagtgcgtga
     1321 tgctacccta caatcgccgg gcatgctgtg gctaaaggcc ttgcacgtgg tgttcgtggt
     1381 cgcctggttc gcggggctgt tctacctgcc gcggctgtat gtctaccatg cggtcaccag
     1441 cgacgccccc gggctcgccc gcttcgtggt gatggagcgg cggcttttcc tgctgatgac
     1501 tatcggcgcg ctgctcgccg tgctcttcgg cctggcgatg atcctcgcct caccgggtta
     1561 tctggcgcag ggctggttgc ggctgaagct gctgctggtc gcgctgctcg tcggttacca
     1621 cttctggtgc taccggctga tgctggcact gcgcctcggg gcgaatccgc actcgcagcg
     1681 ctggtaccgt tggttcaacg agctgccggg ccttttcctc atcgcgatcg tgatcctcgc
     1741 ggtggtgaag ccctggtgaa gccctggcgc acagcggatc tccgctgcgc gagatacacg
     1801 tgtttccgca atagttcacc aaatggtgaa ctatcgcgat gaactcccct gcaaccgcca
     1861 ggtctcacca gatccgcaca cggtccgccg gcttcatgta gagcttatcg cccggctgca
     1921 cgttgaacgc gctgtaccag tcatcgatgt tcggcagcgg cccgttcaca cggaacttcg
     1981 caggactgta ctcgttcgac atcaccatct cgcgcagcgc cccctcgcgg tacttctcgc
     2041 gccagatctg tgcgtaggca agaaagaagc gctgatcgcc gctcaggcca tcgatcaccg
     2101 gtgcgggctg ccccttgagc gagatccggt aggcctcgtg ggccatgttg agcccgccga
     2161 gatcaccgat gttctctccg atggtattgg cgccattcac tttcagtccg ggcagcgcct
     2221 cgaagtcgga gaactgcttg atcaggcgag cggtgcgcgc cgtgaacacc tgcgcgtccg
     2281 cggccgtcca ccagtcacgc atcgcgccat ccggcccgaa cttgcgcccc tggtcgtcga
     2341 atccgtgccc catctcgtgc ccgatgaccg cgccgatcgc gccgaagttg accgcggcat
     2401 cggcattggg atcgaagaat ggcggctgca ggatcgcggc cgggaatacg atctcgttgt
     2461 tcgaggggtt gtagtaggca ttgatctcct gcggggtcat ctgccactcg gagcggtcca
     2521 ctggcttatc gagccgctcc acctggtagt tccactgcca ctcggcggcg cggcgcacgt
     2581 tgccgagcag atcgtcgcgc cgcacgatga gcttcgagta gtccttccac ttgtcggggt
     2641 aaccgatctt cggcgtgaag gcggcgagct tctcgtgggc gcgcgccttg gtctgcggcg
     2701 tcatccagtc gagcgcgtcg atacgctcgc tgagtgccgc ctccaggcct gcgaccagct
     2761 gctgcatctt cgctttcgat tccggcggaa agtatctcgt cacgtacagc tggccgaccg
     2821 cctctccgac cgagtcatcg accgcatcca cggcacgttt ccagcgctcg cgctgctgcg
     2881 gctgtccacg catggtctgg ccgtagaagg cgaagcgcgc ttcatcgaag cgctgcggca
     2941 ggtagggcgc atggctggtg agatagtgga aggtcaggaa gtccctgaga gtcgcgacgt
     3001 cagtgcgccc gaagaccgcg gcgaggccgc ggatcgcagt gagctcgccc agcaccacct
     3061 gctcgcgggc accgagctgc tgcgcggcga ggaacggctg ccaggggaag cccggagcgt
     3121 acgcgagcag ctgcgccttg ctgcgcgggt tgtagatcgc atcgacgtcg cggcgcttct
     3181 cgagcggcca ttgcaccttc gacgcctcgg tctcgaaggc catgatgccg cgcgctttcg
     3241 ccgccgcgtc gactgtgccg ccgagtgtca gcatctgctc gatgtaggcg acgtacttgg
     3301 cgcgcaactc cttgagcgca gggtcgtcct tcaggtagta gtcgcggtcg ggaagaccga
     3361 gagtcgactc gctgatgaac acgctgtagc gatcggggtt cttgaagtca ggcggcagct
     3421 gcacatcgaa gagcgtggcg aatcccggct cgccgaaaag atgagcgacc tcctcgcgcg
     3481 tgcgtagcgc cgcgatacgc tgcaggtccc cctgggcggg agcgaggccg ttggtctcga
     3541 tcgcagcctg gtccatgtag gcagcgtagt agtcgccgat ctgctgctcg ggggtgccgg
     3601 cgggggcgtg tgcggctgcg gctttctcga tgatcgcgcg cacgcgttcc ttagagagct
     3661 catccaggcg atcgaaggga ccaaagctcg atttgtcggc cggtatggtg aagcgctggt
     3721 accagccgcc attggcatag gcgaagaagt catcgcccgg cctcaccgtc gggtcgaggg
     3781 cgctcaggtc gaggccgaaa tcgccgatgg ctacgctcgt gcgtggtgcc gcctcgcccg
     3841 cccgggcgct ggttgcggca gcggcgccgg ccgcgggcgc ggccgcttga gtgttgggca
     3901 gcacggattt ctcctcgacc ggcttgccgg cgcagcccac gagggccagc agcgcagcca
     3961 tccacagggg tctcatgatg tcgcaaaacc ttcgattcat acggggcgcg caatgatacg
     4021 ggagtggcgc ggcctgcggt gcacgggtgg ccgcgcgtcc ccggctcatc gtctcaggcg
     4081 ggcagctccc ggacgcgatc gcgccactcg gtgcgtgccg cgggggcgcg ctcgccgctc
     4141 tcggtgccga agtacagaaa accgacgatg gcgtccgccg cttcgagtcc gagtgcctgc
     4201 ttgaccgcgg catcgtaggc cgcggcaccg gtcttccaca tggcattgaa gccgagtgcg
     4261 aaggccgcga gcatcatggc gtgcgcggcc gcgccggccg agagcgtctg ctcgatgacc
     4321 ggtatcttca ccgccggctc gcagtgggcg gcgaccacga tgatcagcgg cgcgcggaag
     4381 gccttgagcc gctcgcgctg tagcgcctct tgcgcgctct gcgggcgggt acggcgcagt
     4441 tgttcggcga gcagctcacc gaagcgctcg cgggccgcgc cgcgcaccac cacgaagcgc
     4501 cagggccgca atcggccgtg atccggggcg cgggcagcgg cggagaagat gagctcgagt
     4561 gcgccgttat cgggcgccgg ttcgatgagc gcgcgcgccg agcggcgcgt caacagggcg
     4621 tctatggctt gcatgagcgg attgtcgcag actgctggga gtatcaacat gttgataccc
     4681 ccgcatatca acatatttat atgctcaccc agtcggaagc ctgcgagcgc aacaaaaaac
     4741 cgatcctcgc ggtactgcgg gaggagctcg tcgcctgcac ccgggtgctc gaggtcggca
     4801 gcggcacggg gcagcacgcc gtgtatttcg cggccgcgct ggcgcagctc atctggcagc
     4861 cgagcgagct ggcggagaac ctgccctcgc tcgaggagcg catacggctc gagggcggcg
     4921 cgaacctgct cgcgccggtg gcgctcgacg tggtcgctga tccctggccg gtggcaccgg
     4981 tcgatgccgt cttcagcgcc aacactctgc acatcatggg atggagcgcg gtgtgcgagt
     5041 tctttcgcgg cgcggcggag gtgctggcgc ggcccggcgc gctgtgcgtg tacgggccgt
     5101 tccgcttcgc aggacgccat acgagcgaaa gcaacgtcgc cttcgatcag tggctcaaac
     5161 agcgcgatcc cgcaagcggg gtgcgggact tcgaggcgct cgaccggctc gcacagcagc
     5221 gcggactcgc cctgacggcg aaccatgcga tgccggccaa caaccacacg ctggtctggc
     5281 ggaaggcgta gagtcttgtc catggacccg gacgcgctgc gcgctatccg cgaggcgatt
     5341 cgcaccattc ccgactaccc caagcccggg gtgatgttcc gggacatcac cacgctcctt
     5401 ggcaacgcgc acgcctttca ccaggtggtg gaggagctcg cccgcccctg gcgcaacaca
     5461 caggtcgatc acgtcgcagg catcgaggca cgcggtttca tcctcggcgg ggcgatcgcc
     5521 catcagctgc acgccggctt cgtgccgatt cgcaagaagg gcaagctgcc tcacaccacg
     5581 gtacgcgtcg cctactcgct cgagtacgga ttggatgaaa tggagatgca ccgcgatgcg
     5641 gtctcgcccg gcgagcgcat cgtgctggtg gacgatctga ttgcgaccgg cggcacggcg
     5701 gaagccgcgg tgcgactgct gcactcgctc ggcgcgagca ttcttgccgc gtgcttcatc
     5761 atcgatctgc cggacctcgg tggctcgacg cgcctcgcgg cgctcgatgt gccggtgcgg
     5821 acacttgttg cctttcacgg acactaggca ccgggcacgc cgacggcgcc acctgcggaa
     5881 ttacattttc catcagaggg ttacgcccag atcaccgtca acattacgtt gcggtcatat
     5941 gccgcttcgg ggtggatatt ccagtcataa tagtggcggc agtgcatccc ttacttttgg
     6001 agaccatatg ttcaagaagc tcgtcccagt cattgctctc gccctgctcg cgggcccggt
     6061 ctttgcagcc gatgcaccgg ccagcacgcc cgctgccgat cagtcgagca cggcttcgtc
     6121 caagcatcac aagaagagcc atagcagcag ccatagcagc agccaccaca agaagtcgac
     6181 gacgaccgac gcgtcatcga ccgcccccgc caagtaacgg tcagccgact ggcgttgtga
     6241 aagccccgct caggcggggc ttttgctttt ggggcttgcg gctttggcca aaccggttca
     6301 aatttcggtc atggatggga cgcaacaggc cgcaatacat caggcgctgg tttccgttca
     6361 acttgccgtc acgagcatga ccttcccgag ctgcgatcag gacgatctga tcgaagccat
     6421 cgatcgcgtc gaagagcaac tccatgcgag tcatccgaac ctggcactgg tgtgccggtt
     6481 cctcaactcg attgcgagaa gcctgcgcgc gcagccggaa gcccgtgacg cttgcctcct
     6541 catcgaggac gcgatcggca aggccggagt ccccagcacc tggcagtcgg ggatctgacc
     6601 gacccgacgc ccgcacccgc ctcgcgagcc gccggtgcgc ggctcgtcaa ctcacctcat
     6661 tccccggcat tgccggcgag cctgaaagga tagcccgccg aatagttcac cgtttggtga
     6721 actattcccg agaagctcat cgcctgcggg gcaagccccg tccccgacgt acacaccgct
     6781 cccgccggcc ccgcgctgca gcgttgcgcc tgcgcacgcg cgcggtctaa gttcactcca
     6841 tgttcacgcc tatcgcgcag tcgctcattc tgctggcggg ctcggtgttc ctgatcacgc
     6901 tggtgcggcg gctgtcgctg ccgaccagcc ttgcgtacct gctggtcgga ctcgtgctcg
     6961 gtccgcacgc gctcggagtg gtatccgact cgggcacgac gcggctgctg gcggagctcg
     7021 gagtcgcctt cctgctgttc acgctcgggt tggaattctc gctgccgcgc atgctcgcga
     7081 tgcggcgcga ggtgttcggg ctcggcgcgc tgcaggtggt cgccaccacc gcagtgttcg
     7141 cgctgatcgg ccacctgctc ggcatacgct ggctcctggc ggtcgtgctc ggcggcgcgg
     7201 tcgcgatgag ctccaccgcc atgttgctgc agcagctcac cgagcgggcc gagctcaatc
     7261 gcacccacgg ccggctggcc ttcgccatgc tgctcttcca ggacctggcg ttcgtaccgt
     7321 tttttgcgct cggcgcgctg ctgagcgcgg gccgcgagcg gttcgcggtc agcgagagtc
     7381 tcgaggcgct cggcagcgcg gtgctggcgc tcatcggcgt gctggccgcc gggcgctggc
     7441 tgctgcggcc gctgtttcac gagatctcgc acagccgcct gcgcgagctg ttcacgctca
     7501 cggtgctgct ggtggtgctc tcctccgcct gggtctcgca cctttccggc ctgtcgttcg
     7561 ccctcggtgc tttcctcgcc ggcatgatgc tgggggagac cgagtaccgc tatcagatcg
     7621 atacggccat ccggccgttc cgtgacctgc tgctcgggct gttcttcgtc tcggtgggaa
     7681 tgctgctcaa cctgcatctg ctgtggcatc cgagtgagct cgcgatcgcg ctcgccatgc
     7741 tcgtgacgct cgtggtgctg aaggccgtac tcgcagccct cgcgacctgg cccttcacgc
     7801 actcgcgctt caaggcgttg cgcaccggca tcgtgatgtc gatcggcggg gagttcggca
     7861 tagccctgtg cacggtgctg ctgcaggcgc gactcgtgcc cgaaggactg gttgagccgc
     7921 tgctggtggc gatcgtgctg tcgatgctgc tcagtccctt catcctcaac aacaacaagc
     7981 gcgtcgcgcg ggcgttgctg cgcgagcgcg ccccggagcc
//
LOCUS       DATDMP010000272         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_375475, whole genome shotgun sequence.
ACCESSION   DATDMP010000272 DATDMP010000000
VERSION     DATDMP010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375475"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VI195_04645"
     CDS             complement(<1..848)
                     /locus_tag="VI195_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6823703.1"
                     /translation="MPRPLGRLRLFALLVALLNGAALAADRTADAGGSAADALARDIF
                     QQLIEINTTDSVGNVTTAAEALAQRFRAAGWAAGDVVIAGPTERKKNLVVRLHGTGKR
                     RPVLLIGHLDVVEARREDWSTDPFQLIEKKDGYFYGRGTLDMKSGDAIMTTALLRMRR
                     EGYRGSRDIILALTADEEGGCCNGVEWLAHSQRELIDAEFVLNHDGLVGYSITSEHGV
                     PQEFDLSATEKTYADYQLSVTNRGGHSSLPRPDNAIYELAESLLRIGRYAFPFELNEI
                     TRAYFER"
     gene            complement(873..2318)
                     /locus_tag="VI195_04650"
     CDS             complement(873..2318)
                     /locus_tag="VI195_04650"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6823704.1"
                     /translation="MTARSRICAAAAALLLTALAAAALAVAAEGHSASNALARELLEQ
                     LVAINTTDTPRGNVTTAAEAMAQRLRSAGFAAEDIQVLGPNERKRNLVVRLRGSGQHA
                     AVLLIGHLDVVEARREDWSSDPFQLVEKDGYFYGRGTLDMKGPDAIMLAALIRLKHER
                     FRPARDITLALTADEEGGGANGVEWLLRHHRQLIGAQFVLSGDDYSVLTENGKPLFFK
                     LVASEKVYADFQLRVTNKGGHSSEPVADNAIYTLTHGLDRLAAYEFPFELNAVTREYY
                     RRLAQIESGQKAADMRAVLRNPPDAAALGRLSQDPVERAITHTTCVATRLEAGHANNA
                     LPQRAQAVINCRILPGHSPEEVRQQLVQVLGNPAITVDYLANDGTLLAEAPSGGGFAP
                     PPLLPEMLTPLDRLVAQTWPGLKVIPSMSAGASDATHTSAAGLPTYTFSALTVDPNDD
                     REHGRDERIGVASFYQANEFFYRYLRTLTAP"
     gene            complement(2315..3382)
                     /locus_tag="VI195_04655"
     CDS             complement(2315..3382)
                     /locus_tag="VI195_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY6823705.1"
                     /translation="MQPGTSRAPRYAWLAGAGALWLTAISAHGQAAPQYRIAHEVSLP
                     GDGGWDDLTFDDGGHRLFIAHGNQVQVIDADKLALAGTIADTPGVHAIALAGDVGHGY
                     ISAGRSGTIVVFDLKSLARLKEIKATGENPDAILYDSATHRVFSFNGRGRNATVVDVK
                     TDEVIGTIPLDAKPEFAVSDGKGALYVNLEDKSSIAQIDAQHLKVSAVWPIAGCEEPS
                     GLAVDLPGRRLFAGCSNKVMAVIDLRTGHTLGTAPIGAGVDGAKYDSGTHQAFASCGE
                     GVLSVIATGASGKPEVAQSLPTQRGARTMALDEHAHRLFLVTAGLGPPPAATADNPHP
                     RPAILPGSFRLLVAAPANLTQ"
     gene            complement(3400..>3590)
                     /locus_tag="VI195_04660"
     CDS             complement(3400..>3590)
                     /locus_tag="VI195_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823706.1"
                     /translation="VVGAYYYLRVIKLMYFDEPAGTQPSATREPGVRFALGVNAAAVL
                     ALGLLPGPLLDLCARLIH"
BASE COUNT          575 a         1202 c         1212 g          601 t
ORIGIN      
        1 cgctcgaaat aggcgcgagt gatctcattg agctcgaacg gaaatgcgta ccggccgata
       61 cggagcagac tctcggcgag ctcatagatc gcgttgtcag gccgcggcag ggagctgtgg
      121 ccacccctgt tggtcaccga cagctggtag tcggcgtagg ttttctccgt agcactgaga
      181 tcgaactcct gcggcacgcc gtgttcggaa gtgatcgagt atccgaccag cccgtcgtga
      241 ttgagcacga actcggcatc gatgagctcc cgctggctgt gggccaacca ctcgacgccg
      301 ttgcagcagc cgccctcctc gtccgcggtg agcgcgagga tgatgtcacg gcttccccgg
      361 tagccttccc gccgcatgcg cagcagcgcc gtggtcatga tggcatcccc gctcttcatg
      421 tcgagcgtgc cgcggccgta gaaatagccg tccttcttct cgatgagctg gaacgggtcg
      481 gtgctccagt cctcgcgccg cgcctccacc acatcgagat gcccgatgag cagcactggc
      541 cggcgcttgc cggtgccatg caaacgcacg accaggttct tcttgcgctc cgtgggtccc
      601 gctatcacta catcgccagc ggcccagccc gcggcgcgaa agcgctgcgc cagcgcctcg
      661 gcggcggtgg tgacgttgcc cacggaatcg gtcgtgttga tctcgatcag ctgctggaag
      721 atatcgcgcg ccagcgcatc cgcagctgac ccgcctgcat cggccgtgcg gtctgcagcg
      781 agcgctgcgc cgttgaggag cgcaacgagc agagcgaaca atcgcaagcg gccgagggga
      841 cgtggcatgt ggtattacct cgcgcggcgg gctcagggtg ccgtcagcgt ccgcagatag
      901 cggtagaaga actcgttcgc ctggtagaac gaggcgacgc cgatgcgctc gtcgcgaccg
      961 tgctcgcggt catcgttggg atcgacagtc agggccgaga aggtgtacgt cggcagcccc
     1021 gcggcgctcg tgtgcgtggc gtcggaagcg ccggccgaca tggaagggat caccttcagc
     1081 cccggccagg tttgcgccac cagccgatcg agcggcgtca gcatctcggg cagcagcggc
     1141 ggcggcgcga acccgcctcc gctcggggct tcagcgagca gcgtgccatc atttgcgagg
     1201 tagtcgacgg tgatcgccgg attgcccagc acctgcacga gctgctggcg cacctcctct
     1261 ggcgagtggc cgggaaggat gcggcagttg attactgcct gggcccgctg cggcagcgca
     1321 ttgttggcat ggccggcctc gagacgcgtc gcgacgcagg tggtgtgggt aatggcacgt
     1381 tcgaccgggt cctgtgacag ccgcccgagc gccgcagcgt caggcggatt gcgcagtacg
     1441 gcccgcatgt ccgccgcctt ctgcccggac tcgatctgcg caaggcgccg atagtactcg
     1501 cgcgtcaccg cgttgagctc gaagggaaac tcgtacgcag cgagacgatc gaggccatgg
     1561 gtcagggtgt agatggcgtt gtccgcaacc ggctcggagc tgtgcccgcc cttgttggtg
     1621 acgcgcagct gaaagtccgc gtaaaccttc tcgctcgcca ccagcttgaa gaacagcggc
     1681 ttgccgttct cggtcagcac cgagtagtca tcaccgctca gcacgaactg tgcaccgatg
     1741 agctgccggt gatgccgcaa cagccactcg acgccgttgg caccaccgcc ttcctcgtcg
     1801 gcggtgagcg ccagggtgat gtcgcgtgcg ggcctgaacc gctcatgctt cagccggatc
     1861 agagccgcca gcatgatcgc gtccggcccc ttcatatcga gcgtgccgcg gccgtagaag
     1921 tagccgtctt tctccaccag ctggaacgga tcgctgctcc agtcctcgcg ccgcgcctcc
     1981 accacatcga gatgaccgat gagcagaacc gccgcgtgct gaccggagcc gcgcaggcgc
     2041 accacgaggt tcctcttgcg ctcgttgggg ccgagtacct ggatatcctc ggccgcgaag
     2101 cccgcgctgc gcagccgctg cgccatcgcc tcggcggccg tcgtgacgtt gccccgggga
     2161 gtgtccgtgg tgttgatcgc gaccagctgc tcgagcagct cgcgtgccag tgcattggag
     2221 gcactgtggc cttccgcggc caccgcgagt gccgccgccg caagggcggt cagcagcagc
     2281 gccgccgccg cggcgcagat gcgggagcga gcggtcactg ggtgaggttc gccggagccg
     2341 ccaccagcag acggaacgat cctggcagga tcgccggccg gggatgggga ttgtcggcgg
     2401 tcgccgcggg cgggggaccc aggcccgcgg tgacgaggaa gagccgatgc gcgtgctcat
     2461 cgagcgccat ggtgcgcgca ccccgttgcg tcggcagcga ttgtgcgacc tcgggcttgc
     2521 cgctggcgcc ggtcgcgatc accgacagca caccttcacc acaggaggca aaggcctgat
     2581 gcgtgccgga gtcgtacttc gcgccgtcga cacctgcgcc aatcggcgcc gtgccgagcg
     2641 tgtgtccggt gcgcaggtcg atcaccgcca tgaccttgtt gctgcacccg gcgaacaggc
     2701 gccggccggg gaggtcgacc gcgagtccgg agggttcctc gcagccggcg atcggccaga
     2761 ccgcgctcac cttgagatgc tgggcatcga tctgcgcgat gctgctcttg tcctcgaggt
     2821 tcacgtagag cgcgcccttg ccgtcgctca ccgcgaattc cggcttggca tcgaggggga
     2881 tcgtgccgat gacctcatcg gtcttgacat cgaccaccgt cgcattgcgc ccgcggccat
     2941 tgaagctgaa aacccggtgg gtcgcggaat cgtagaggat cgcgtccggg ttctcgcccg
     3001 tggccttgat ctccttcagc cgcgcgagcg acttcagatc gaacaccacg atggtgccgc
     3061 tgcggccggc actgatgtag ccatgtccca catcgccggc gagcgcgata gcgtgcaccc
     3121 ccggggtatc ggcgatggtt ccggcgaggg cgagcttgtc ggcgtcgatg acctgcacct
     3181 gattgccatg ggcaatgaag agccggtgtc ccccgtcatc gaaggtcaga tcgtcccagc
     3241 cgccatcacc cggcaggctt acctcgtggg cgatgcggta ctgcggcgcc gcctgcccgt
     3301 gcgcgctgat cgccgtgagc cacagtgcgc ccgcgcccgc cagccaggcg tagcgcggcg
     3361 cccgggatgt tccaggttgc atcgcgagga ctctccagtt cagtgaatca gccgcgcaca
     3421 caggtcgagc agcggaccgg gcaggagccc gagcgccagc actgccgccg cgtttactcc
     3481 gagagcgaaa cgcacacccg gctcgcgcgt cgccgacggc tgcgtgccgg cgggctcatc
     3541 gaaatacatc agcttgatga cacgcaggta gtagtaagcg cctaccacgg
//
LOCUS       DATDMP010000273         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1597634, whole genome shotgun sequence.
ACCESSION   DATDMP010000273 DATDMP010000000
VERSION     DATDMP010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597634"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VI195_04665"
     CDS             complement(<1..774)
                     /locus_tag="VI195_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6823707.1"
                     /translation="MALVTRTSGRRLVAGIPTLWLLLLFLIPFLIVFRISFSEVRLAI
                     PPYTPLISWHHGSPTLEVHTSAYSFLFTDPLYVSSYLYSLKVAAVSTLCCLLIGYPMA
                     YAIARARPATRSVLLMLIVLPFWTSFLLRVYAWIGLLKNNGVINNVLLSLGVIHQPLA
                     LLQTDFAVYIGIVYSYLPFMILPLYTNLEKHDPTLLEAAADLGARPLRAFLAITLPQS
                     LAGIFAGSLLVFIPAVGEYVIPTLLGRTDQLMIGRVLSDE"
     gene            complement(783..1949)
                     /locus_tag="VI195_04670"
     CDS             complement(783..1949)
                     /locus_tag="VI195_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6823708.1"
                     /translation="MNDMNALPETNGPNAPAGSERDAAYVRIANLTKKFGDFAAVDAV
                     SLDIRRGEIFCLLGGSGSGKTTLLRMLAGFERPSAGTIHIDGVDMSLIPPYERPVNMM
                     FQSYALFPHMSVEKNVAFGLEQEGLGREPIRRQVGEILDIVQMSGLGGRKPHQLSGGQ
                     RQRVALARALVKRPKLLLLDEPLAALDKKLRERTQFELLNIQQRLGVTFIVVTHDQEE
                     AMTLGQRLGVMNHGRIAQVGTPSDIYESPATRFVADFIGSVNMFEGRVSEERAEGVRL
                     EVGELGCMLRAERSLSCGRGTTVWAAIRPEKISISRQPPPAAAAAMPENAVRGTVREI
                     AYMGDMSIYLVQIASGKMLRVTLPNTTRGGERPIAREESVWLSWHGSSPVVLTE"
     gene            complement(1964..>2478)
                     /locus_tag="VI195_04675"
     CDS             complement(1964..>2478)
                     /locus_tag="VI195_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY6823709.1"
                     /translation="NSNSPADLKAAEQVLHAMRPYVRYVDSSRYIDNLANGDICLVMG
                     WSGDVKQAHDRAQEAGKGELAYNIPREGAIINYDVMAIPADAPHVRNAHLFINFLLRP
                     DIAARNSNLIKYPSAVRPDLQPLDPAVRNDPTVYPPPAVRARLTPERPRPADYQRLLT
                     RMWTRFKTGK"
BASE COUNT          443 a          789 c          845 g          401 t
ORIGIN      
        1 ctcatccgac agcacccggc cgatcatcag ctgatcggtg cgcccgagca gcgtcgggat
       61 cacgtactca ccgaccgccg ggatgaacac cagcagcgat cccgcgaaga tgccagcgag
      121 cgactgcggc agcgtgatgg cgaggaatgc gcgcagcggc cgcgcgccga ggtccgcggc
      181 cgcttcgagc agcgtcggat cgtgtttctc gaggttggta tagagcggca ggatcatgaa
      241 cggcagatag gaatagacga taccgatgta gaccgcaaaa tcggtctgca gcagcgcaag
      301 cggctggtgg atcacgccga gcgagagcag cacgttgttg atcacaccgt tgttcttcag
      361 caggccgatc caggcataca cgcgcagcag gaacgacgtc cagaagggca gcacgatcag
      421 catcagcagt accgagcgcg tcgccggccg cgcgcgcgcg atggcgtagg ccatcgggta
      481 gccgatcaac agacagcaca gcgtcgatac tgcggccacc ttgagcgagt agaggtagga
      541 gctcacgtac agcgggtcgg tgaacaggaa cgagtacgcc gaggtgtgca cttcgagcgt
      601 cgggctgccg tggtgccagg aaatgagcgg tgtgtagggc ggaatggcca ggcgcacctc
      661 ggagaaggag atgcgaaaca cgatcagaaa cggaatcagg aacagcagca gcaaccacag
      721 ggttggaatc cccgcgacca gtcgccgccc ggaggtgcgc gtgacaagcg ccaaaattac
      781 tcctactcgg tgagcaccac cggactcgag ccgtgccagg agagccacac gctctcctcg
      841 cgcgcgatcg gccgctcgcc accgcgcgtg gtgttgggca gcgtcacgcg cagcatcttt
      901 ccggaggcga tctgcaccag gtagatcgac atgtccccca tgtaagcgat ctcgcgcacc
      961 gtgccgcgta ccgcattctc gggcatggcc gcggccgcgg ccggcggcgg ctgacggctg
     1021 atgctgatct tctccgggcg gatcgcagcc catacggtcg tgccacgccc acaactcagc
     1081 gagcgctccg cgcgcagcat gcagccgagc tcgccgactt ccaggcgcac accctcggcg
     1141 cgctcctcgc tgacccgtcc ctcgaacata ttgaccgagc cgatgaaatc cgccacgaag
     1201 cgcgtggccg gggactcgta gatgtccgac ggcgtgccga cctgcgcgat gcggccgtgg
     1261 ttcatgaccc cgagccgctg cccgagcgtc atggcctcct cctggtcatg ggtgaccacg
     1321 ataaaggtca cgccgagccg ctgctggatg ttgagcagct cgaactgggt gcgctcgcgc
     1381 agcttcttgt cgagcgcggc gagtggctca tcgagcagca gcagcttcgg gcgcttcacc
     1441 agcgcgcggg ccagcgctac gcgctggcgc tgcccgccgg agagctggtg cggtttgcgc
     1501 ccacccaggc cgctcatctg cacgatatcg aggatctctc ccacctggcg gcggattggc
     1561 tcgcgcccga gcccctcctg ctcgagtccg aaggcgacgt tcttctccac gctcatgtgc
     1621 gggaagagcg cgtaagactg gaacatcatg ttcaccgggc gctcgtaggg cggaatgagg
     1681 ctcatgtcga cgccatcgat gtggatggtg ccggcgctcg gacgctcgaa gcctgccagc
     1741 atgcgcaaca gcgtggtctt gcccgagccc gagccgccga gcaggcagaa aatctcgccg
     1801 cggcgaatat ccagagagac ggcatcgacc gcggcgaagt cgccgaactt cttggtgagg
     1861 ttcgcgatgc gcacataggc ggcgtcgcgc tcgctgccgg cgggcgcgtt cggaccattg
     1921 gtttcgggca gcgcgttcat gtcgttcatc gcaggccttg ggctcactta ccggtcttga
     1981 agcgcgtcca catgcgcgtc agcagccgct ggtagtccgc cggccgtggc cgctccggcg
     2041 tcaggcgggc acgcaccgcg ggcggagggt agacggtcgg atcgttgcgc actgcggggt
     2101 cgagcggttg caggtcgggc cgcaccgcgc ttgggtactt gatgaggttg gagttgcgcg
     2161 cggcgatgtc cgggcgcagc aggaaattga tgaacaggtg cgcgttgcgc acgtgcggcg
     2221 catctgcggg gatcgccatc acgtcgtagt tgatgatcgc gccctcgcgc ggaatgttgt
     2281 atgccagctc gcccttgccg gcctcctgcg cgcgatcgtg cgcctgcttg acatcgcccg
     2341 accagcccat caccaggcag atatcgccgt tggcgagatt gtcgatgtag cgcgaggagt
     2401 ccacgtagcg cacgtaggga cgcatggcgt gcagcacctg ctcggcggcc ttgagatcgg
     2461 cgggcgagtt gctgttgg
//
LOCUS       DATDMP010000274         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_154210, whole genome shotgun sequence.
ACCESSION   DATDMP010000274 DATDMP010000000
VERSION     DATDMP010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154210"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VI195_04680"
     CDS             <1..1208
                     /locus_tag="VI195_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY6823710.1"
                     /translation="GIANLAMATGNLGREGVGVSPLRGQNNVQGSCDMGSFPHEFSAY
                     RHVSDPAVRQSFEKEWGVQLQSEPGLRIPNMFEAALDGTFKGMYVQGEDFVQSDPNTH
                     HVNAAMASLECCIVQDLFLNETAKFAHVFLPGSSFLEKDGTFTNAERRTSLVRKVIEP
                     LPGYQDWEITCLISNALGYPMHYANASEIMEEIARLTPTFAGMSHERITQLGSIQWPV
                     NDAAPEGTPTMHVGQFVRGKGKFWQTEYVPTSEKVNTRFPLILTTGRILTQYNVGAQT
                     RRTGNVIWHKEDVLEIHPHDAEVRGIADGDWVGLTSRAGDTVLRAKVSEKIAPGVVYT
                     TFHFPETNANVVTTENSDWATNCPEYKVTAVEVVLVNQKQAWEKRAEAERALPRRAPK
                     RRRQDRTPA"
     gene            1213..2043
                     /gene="fdhD"
                     /locus_tag="VI195_04685"
     CDS             1213..2043
                     /gene="fdhD"
                     /locus_tag="VI195_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY6823711.1"
                     /translation="MSAQPEANGQTPAAVSAELPVQRWTRGALASTTDLIAEEMPVAL
                     VYHEVPHVVMLATPADLEDYAYGFTLSEGLVASVDEIRGVEVVQGAASADVKISVAWE
                     RFTQLLQRRRNLAGRTGCGLCGAETAADAIRECPPVPPGISLSAAELHAAIAQLSGRQ
                     PINARTGSVHAAAWVVPGRGIEAVREDVGRHNALDKTIGALARARADFAAGYMLITSR
                     ASYEMVQKCATVGIAVLVALSAPTAFAVRLAQRAGLTLVAFARADQHVVYAHPQRLRN
                     "
     gene            2058..2369
                     /locus_tag="VI195_04690"
     CDS             2058..2369
                     /locus_tag="VI195_04690"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEY6823712.1"
                     /translation="MNIDLLIKMANEIGDFFAGADAHDPQAAARDVANHIKRYWEPRM
                     RAQMLKYYEERGGAGLTDLGKSAVALLYAASQAAAAANPSAAAAAPTPPPAAPAKGRG
                     A"
     gene            2366..>2824
                     /locus_tag="VI195_04695"
     CDS             2366..>2824
                     /locus_tag="VI195_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY6823713.1"
                     /translation="MSSATRAGGRALICGSLAFDTIMVFGDRFANHILPDMIHILNVS
                     FLVPQMRREYGGCAGNIAYNLALLGDPGVPMATVGRDFGAYHEWLARAGVPTEHVRVI
                     DSELTAQAFITTDLDDNQITAFHPGAMQHSHANRVSEARDIALGIVAPDGR"
BASE COUNT          486 a          976 c          951 g          411 t
ORIGIN      
        1 tgggcatcgc caacctcgcc atggccaccg gcaatctcgg ccgcgagggt gtcggcgtgt
       61 cgccgctgcg cggccagaac aacgtgcagg gctcgtgcga catgggctcg ttcccgcacg
      121 aattcagcgc ctaccgccac gtgtcggatc cggcggtgcg ccagtcgttc gaaaaggaat
      181 ggggagtgca gctgcagtcc gagccgggtc tgcgcattcc caacatgttc gaggccgcgc
      241 tcgatggcac cttcaagggc atgtacgtgc agggcgagga cttcgtgcag tccgatccca
      301 acactcacca cgtgaacgcc gccatggcct cgctcgagtg ctgcatcgtg caggacctgt
      361 tcctgaacga gaccgcgaag ttcgcgcacg tgttcctgcc gggctcctcg ttcctcgaga
      421 aggacggcac gttcaccaac gcggagcggc gcacctcgct ggtgcgcaag gtgatcgagc
      481 cgctccccgg ttaccaggac tgggagatca cctgcctgat ttccaacgcg ctcggctatc
      541 cgatgcacta cgccaatgcg agcgagatca tggaggagat cgcgcggctc acgccgacct
      601 tcgccggcat gagccacgag cggatcacgc agctcggcag catccaatgg ccggtcaacg
      661 acgcggcgcc cgagggcaca ccgaccatgc acgtcggtca gttcgtgcgc ggcaagggca
      721 agttctggca gaccgagtac gtgccgacca gcgagaaggt caacacgcgc ttcccgctga
      781 ttctcaccac cgggcgcatc ctcacgcagt acaacgtcgg tgcgcagacc cggcgcacgg
      841 gcaacgtcat ctggcacaag gaggacgtgc tcgagattca cccgcacgac gccgaggtgc
      901 gcggcatcgc cgatggcgac tgggtgggac tcaccagccg cgccggggac acggtactgc
      961 gcgccaaggt gtccgagaag atcgcgccgg gcgtggtcta caccacgttc catttccccg
     1021 agaccaacgc caacgtcgtc accaccgaga actccgactg ggcgaccaac tgccccgagt
     1081 acaaggtgac ggcagtggag gtggtgctgg tgaaccagaa gcaagcctgg gagaagcgcg
     1141 ccgaggccga gcgcgcgctg ccgcgccggg cccccaagcg ccgccgacag gatcggactc
     1201 ccgcctgagg cgatgagcgc acagccggag gcgaacgggc agactcccgc cgcggtgagt
     1261 gcggaactcc cggtccagcg ctggacgcgg ggtgcgctcg ccagcaccac cgacctcatc
     1321 gcggaggaga tgccggtggc gctcgtctat cacgaggtcc cacacgtggt gatgctcgcg
     1381 accccggccg atctcgagga ctatgcctac ggcttcaccc tcagcgaagg actggtcgcg
     1441 agtgtcgatg agatccgcgg cgtcgaggtg gtgcagggcg cggcgtcggc ggatgtgaag
     1501 atcagcgtcg cgtgggagcg ctttacgcag ctcttgcagc ggcggcgcaa tctcgccggc
     1561 cgcaccggct gcgggctctg tggagcagag actgccgcgg atgcgatccg cgagtgcccg
     1621 cccgtgccgc cgggtatcag cctgagcgcg gccgagttgc acgcggcgat tgcgcaactc
     1681 tcggggcgac agccgatcaa cgcccgcacc ggcagcgtgc acgccgcggc gtgggtcgtt
     1741 cccggccgag ggatcgaagc ggtgcgcgag gatgtcggcc ggcacaatgc gctcgacaag
     1801 accatcgggg cgctcgcccg tgccagggcg gatttcgccg ccggctacat gctcatcacg
     1861 agccgcgcga gctacgagat ggtgcagaag tgcgccaccg tgggcatcgc ggtgctcgtg
     1921 gcgctctccg cacccaccgc ctttgcggtg cgcctggccc agcgcgcggg gctgacgctg
     1981 gtcgccttcg cgcgggccga ccagcacgtg gtctacgccc atccgcagcg gctgaggaac
     2041 tgaggcatga cgactccatg aatatcgatc tgctcatcaa gatggccaac gagatcggcg
     2101 acttcttcgc cggtgccgac gcccacgatc cgcaggcggc ggcgcgcgat gtggccaatc
     2161 acatcaagcg ctactgggag ccgcgcatgc gcgcgcagat gctgaagtac tacgaggagc
     2221 gcggcggcgc cggtctcacc gatctcggca agagcgccgt ggcgctgctg tacgcggcga
     2281 gccaggcagc ggcggcggcg aacccctctg cggcggcggc cgcgcctacc ccaccccccg
     2341 cggcgcccgc caagggcaga ggcgcatgag ctccgccacg cgcgcgggcg ggcgtgcgct
     2401 gatctgcggc tcactcgcct tcgacaccat catggtgttt ggcgatcgct tcgccaatca
     2461 catcctgccc gacatgatcc atatcctgaa cgtgtcgttc ctcgtcccgc agatgcgccg
     2521 tgagtacggc ggctgcgccg gcaacatcgc ttacaacctg gcgctactcg gcgatccggg
     2581 cgtgccgatg gcgaccgtag ggcgcgactt cggcgcctac cacgagtggc tggcacgcgc
     2641 cggagtgccg accgagcacg tgcgcgtgat cgacagcgag ctcaccgcgc aggccttcat
     2701 caccaccgac ctcgatgaca accagatcac cgctttccat ccgggcgcca tgcagcacag
     2761 tcatgccaac cgcgtgagcg aggcgcgcga catcgcgctc ggcatcgtgg cgcccgatgg
     2821 gcgc
//
LOCUS       DATDMP010000275         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1820480, whole genome shotgun sequence.
ACCESSION   DATDMP010000275 DATDMP010000000
VERSION     DATDMP010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820480"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VI195_04700"
     CDS             complement(<1..663)
                     /locus_tag="VI195_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823714.1"
                     /translation="MNICVLGLWHLGSVTAACMAKLGHSVVGLDFDEHRVASLNAGMP
                     PVFEPGLEELLRAGLAAGGLRFSHTVGAATAGAQILWVTSDTPISNDGDAQPDLVMQQ
                     LERVIPALDAGTLVLVSSQLPIGSTRALQQRAARQRGGDSLSCACVPENLRLGSALND
                     FLRPDRIIAGVDSHSEQRVLQELLGTIGAPIEWMSLESAEMTKHATNAFLAASVTFAN
                     EVA"
     gene            complement(660..1616)
                     /locus_tag="VI195_04705"
     CDS             complement(660..1616)
                     /locus_tag="VI195_04705"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6823715.1"
                     /translation="MRCLVTGCAGFIGSSLTERLLARGDKVLGYDNFSTGRERFLLSA
                     RQHPGFRLVRADLLDARTLTECMRQVEFVYHLAANADVRFGTEHPRKDLEQNTLATHN
                     VLEAMRASGVARIAFTSSGAVYGEALQVPTPEDASFPVQTSLYGASKVAAEALIAAYC
                     AGFGFQSYIFRLVSILGERYGHGHVLDFYRQLRADPARLRVLGDGRQRKSYLYVHDCV
                     EAMLTAVERAHERVNIFNLGCDASCEVKESVGWICRALGVAPQVEYGSGDRGWPGDNP
                     LIILETSRIRALGWSARLDIRAAVLATLEYLRANPDIVDARR"
     gene            1796..>2782
                     /locus_tag="VI195_04710"
     CDS             1796..>2782
                     /locus_tag="VI195_04710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY6823716.1"
                     /translation="MPLFPNRVVALLLVTALAANVIVRGVAPATARITSDFPGYFTAA
                     KVVADRGEVERLYDNPWFQEQMRRYQVGTSEGKFAPFPPPTALLMVPLTPLTPLNALR
                     VVTAVSALCLIGSIILLARALTWSALDAALLVLLSGSAILGALRLGQPYIAVSACCIL
                     GYYAYLRERPKLAGACFGAFTPLKYFPVIFLVYFAFRRRWQVVVGGALTILLIVLVSI
                     AVLGWRIHEQFLASVLGNHLIANLSMQDPFSARFQSFDTLYRRLFIYDARRNPEPLIV
                     APVLSGAARLVTKAAILLAAIATLIRQARVCGAAATGASLGLLGILTLLLAPA"
BASE COUNT          476 a          916 c          900 g          490 t
ORIGIN      
        1 ggctacttcg ttggcgaagg tcaccgacgc cgccaggaag gcattggtgg catgcttggt
       61 catttccgcg gattcgagcg acatccactc gatcggggcg ccgatcgtgc cgagcagctc
      121 ctgcagcacg cgttgctccg agtgcgagtc cacaccggcg atgatccgat ccgggcgcag
      181 gaaatcgttc agcgcgctgc cgaggcgtag attttccggc acgcacgcac agctgagact
      241 gtcgccgcca cgctggcggg cggcacgctg ttgcagggcg cgggtcgagc cgatcggcaa
      301 ctgcgaggac acgagcacca gcgtgccggc atcgagcgcg ggaatgacgc gctcgagctg
      361 ttgcatgacg agatccggct gcgcgtcacc gtcgttgctg atgggcgtgt cgctggtcac
      421 ccacagtatc tgagcgccgg cagtcgctgc gccgaccgtg tgcgaaaagc gcagcccgcc
      481 cgcggcgagg cccgcgcgca gcaactcctc cagccccggc tcgaacacag ggggcatgcc
      541 ggcgttgagg ctcgcgacac ggtgctcatc gaaatcgaga ccgacgacgc tgtgaccgag
      601 tttggccatg caggccgcgg tcactgagcc cagatgccat aggccgagaa cgcagatgtt
      661 catcggcgcg catccacgat gtcgggattg gccctcaggt actcgagggt cgcgagcacc
      721 gccgcgcgaa tgtcgagccg cgcgctccag ccgagggcgc gaatgcggct cgtctcgagg
      781 atgatgagcg gattgtcccc gggccagcct cggtcaccac tgccgtactc gacctgcgga
      841 gccacgccga gcgcgcggca gatccagccg accgactcct tcacctcgca ggaggcatcg
      901 caacccaggt tgaagatgtt gacgcgctcg tgcgcgcgct ccaccgccgt caacattgcc
      961 tcgacgcagt catgcacgta cagataggac ttgcgctgcc gtccgtcccc gagcacgcgc
     1021 agccgggccg gatcggcgcg cagctggcgg tagaagtcca acacgtgtcc atggccgtac
     1081 cgctctccca ggatggagac cagccggaag atgtaagact ggaagccaaa gccagcgcag
     1141 taggcggcaa tgagcgcctc cgcggcgacc ttggacgccc catatagcga ggtctgcacg
     1201 gggaacgacg catcctccgg tgtcggcacc tgcaatgctt cgccgtaaac ggcgcctgat
     1261 gaggtaaagg cgatgcgcgc cacgccgctg gcgcgcattg cctccagcac gttatgtgtg
     1321 gcgagggtgt tctgctccag gtccttgcgg ggatgctcgg tgccgaagcg aacgtcggca
     1381 ttggcagcga ggtgatagac gaactcgacc tgccgcatgc actcggtgag cgttcttgca
     1441 tccagcaggt ccgcgcgcac cagcctgaag ccgggatgct gacgggcgct gagcagaaag
     1501 cgctcgcgtc cggtggagaa gttgtcgtac ccgaggacct tatcaccgcg cgccaacagc
     1561 cgctcggtca gagagctgcc aatgaagcct gcacaaccgg tgacaagaca acgcaactgg
     1621 agttcctatg tgcgtcagcg cgacgccgcg attgacaacg gccggtgcac cacatcatcg
     1681 tccatgatgt ggtccgcctc aaggagccca gcggtcaggc cagccgcggc gagcacagtc
     1741 cgcgctgccg gaggatttta cggcggactg agcgccgcaa accggcgcgc gcctgatgcc
     1801 actgttcccg aacagagtcg ttgcgctgct tctggtcaca gcactggcag cgaatgtcat
     1861 cgtgcgtggc gtagcgccgg caaccgcgcg gatcaccagc gactttccgg gttattttac
     1921 cgccgccaag gtggtcgcgg accggggcga ggtcgagcgg ttatacgaca acccgtggtt
     1981 tcaggagcaa atgcgccgct atcaggtcgg tacctcagaa ggaaagttcg cgccgtttcc
     2041 accgcccacg gcgctgctga tggtgccgct cactccgctc acgccgctta atgctctgcg
     2101 ggtagtgacc gcggtgagcg ccctgtgcct catcggctcg atcatcctgt tggcgagagc
     2161 tctgacctgg agcgcgctgg acgcggcgct gttggtcctg ctgtccggct ccgccattct
     2221 cggcgcattg cgcctcgggc agccctacat tgcggtttcg gcatgctgca tcctcggcta
     2281 ctacgcctac ctgcgcgaga gacccaagct ggccggcgcg tgcttcggcg catttacgcc
     2341 gctcaagtac tttcccgtga tctttctggt ttacttcgcg ttccgcaggc gttggcaggt
     2401 ggtcgtcggc ggagccttga cgatcctcct catcgtgctg gtcagcattg cggtactcgg
     2461 ctggagaatc catgagcagt tcctggcgtc ggtgctcggc aaccatctca tcgcgaacct
     2521 cagcatgcag gatcccttca gcgctcgctt tcagtcgttc gacacactct atcggcggct
     2581 gttcatctac gacgccagac ggaatcccga gccgctgatc gtcgcgccag tgctgtccgg
     2641 cgcggcgcgg ctggtgacca aggccgcgat cttgctcgca gcgatcgcga ccttgatcag
     2701 gcaggcgcgc gtctgcggag ccgctgccac cggagcatcg ctcggactgc tcggaattct
     2761 gacgctgctg ctcgctcccg cc
//
LOCUS       DATDMP010000276         4576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1376804, whole genome shotgun sequence.
ACCESSION   DATDMP010000276 DATDMP010000000
VERSION     DATDMP010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376804"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VI195_04715"
     CDS             <1..1281
                     /locus_tag="VI195_04715"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY6823717.1"
                     /translation="RAFPPWAALTAKERAAILRRWYELLMAHQEDLATLMTAEQGKPL
                     AEAKGEIAYGAAFIEWFAEEGKRLYGDIIPPHQADRRLLVLRQPVGVVAAITPWNFPL
                     AMITRKAAPALAAGCTIVVKPASQTPYSARAAAALAARAGVPAGVLNVVTGVDAAAIG
                     GELTANPAVRKVTFTGSTAVGKKLMAQCAGTVKKLTMELGGNAPFVVFEDADLDAAVT
                     GAIACKYRNSGQTCVCANRLLVQAPVYEAFTRKLVHAVAQLRVGDGLKGPTDQGPLID
                     AGALEKVEQHIADAVGKGARVALGGRRHALGGTFFEPTVVTEVTSTMRMAREETFGPI
                     APLFRFETEQDAIHMANDTEFGLAAYFYTRDLARAWRVAEALEYGMVGLNTGIFSTEV
                     APFGGVKESGIGREGSKYGILDYTELKFVCVGLG"
     gene            1278..2315
                     /gene="hemH"
                     /locus_tag="VI195_04720"
     CDS             1278..2315
                     /gene="hemH"
                     /locus_tag="VI195_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY6823718.1"
                     /translation="MSTERIGILLVNSGTPEAPEPAAVRAFLARFLADRRVVQLPRAL
                     WLPLLYGVILPLRPRRVAPKYRRIWTQAGSPLRELTVRLRAELAGVLAQRMLAPLQVE
                     LGMLYSAPHVGGALERLRAGGAQRLLVLPLFPQYCGATTGAAFDQVSAALQRLRWLPD
                     LQLIADYHDHPGYIEALRASIVAHWKAHGRTPHLLLSFHGIPERYCHQGDPYHKRCHA
                     TARLLADELLLQEGEWSVSFQSRFGPAQWLRPYTQAVLGAMPARGVREVTVVCPGFAV
                     DCLETLEEIAIENRARFSQAGGQRFEYVPALNAATAHAQCLADLVAQRCQSWTETLRA
                     HLPGTAHGAPA"
     gene            2315..3055
                     /locus_tag="VI195_04725"
     CDS             2315..3055
                     /locus_tag="VI195_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823719.1"
                     /translation="MSHAPLPLAGIFHEFGVAVAEVRSAVEFYERLGFSQATTTDTFS
                     HPYGVLTDGRFCIGLHQRAGVSPILTFVRPGIAGSVAALAAAGIQLTRCQTGEEVFNE
                     IGFSDPFGHELAILEARTYSPVARDAVEVSLCGDFAEVSLPAADFSVAQTFWEPLGFV
                     AAEQAQSPYPHLTLTSDHLDLAFHSPQLCASPMLVFHAADMSARLERLRTLGFAIASA
                     PARVDAGAGNALLIGPGNTPLLLLEAAD"
     gene            complement(3086..3865)
                     /locus_tag="VI195_04730"
     CDS             complement(3086..3865)
                     /locus_tag="VI195_04730"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY6823720.1"
                     /translation="MSARCKLPLAAALFLAALCGCPRPERVAPPAPQSAPAGSAAAVV
                     PSAPAPHLGTPHPILAQSSLLTVLVYRGGALASAGHNHVIASHDLSGTIYVPQQIMQS
                     SFELHIPVATLTVDEAALRAQQPAAEFPADIPEGAKAGTRRNMLSDALLDAQRNPEIV
                     LQSLQLAPAGGAADSATVLAQVQSSVRGQLRSFTVPVRYRLAGNGTLEATGEFPLRQT
                     DLGLTPFSAMLGALQVQDEMRVSFHIIAQAAAPAAARPGRH"
     gene            3884..4453
                     /locus_tag="VI195_04735"
     CDS             3884..4453
                     /locus_tag="VI195_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY6823721.1"
                     /translation="MSLRNTSHRWGLLSQLFHWLIVVLIILQVTLASLADDLPPGAKK
                     LALLARHKSVGITILMLVILRLLWRSLNLTPLLPTTLKPYERVLARCTHAALYVLLFA
                     MPLSGWTMSSARGFPVSWFGFFTLPDLVPKNKSLYAALLTTHEVLAWVLAAVVTLHVA
                     AALKHHFMLRDDVLRRMLPFTTTGEGPKA"
BASE COUNT          671 a         1607 c         1548 g          750 t
ORIGIN      
        1 cgggcttttc cgccctgggc cgcgctcacc gccaaggaac gggcggcgat cctgcgccgc
       61 tggtacgagc tgctgatggc gcaccaggaa gatctcgcca cgctcatgac ggccgaacag
      121 ggcaagccgc tcgcggaagc caagggtgag atcgcctacg gcgccgcctt catcgaatgg
      181 ttcgccgagg agggcaagcg cctctacggc gacatcatcc ccccgcatca ggcggaccgg
      241 cggctgctcg tgctgcgaca gccggtcggc gtggtcgccg ccatcacacc ctggaatttc
      301 ccgctcgcga tgatcacgcg caaggcggct cccgcgctcg ctgccggctg cacgatcgtg
      361 gtgaagcccg ccagccagac gccctactcg gcgcgcgccg ccgcggcgct cgccgcgcgc
      421 gccggggtcc cggcgggcgt gctcaatgtg gtgacgggcg tcgatgcggc ggcgatcggc
      481 ggcgagctca ccgccaatcc cgcggtgcgc aaggtcacct tcaccggttc gaccgccgtc
      541 ggcaaaaagc tcatggcaca gtgcgccggt acggtgaaga agctcaccat ggagctcggc
      601 ggcaatgcgc cgttcgtcgt cttcgaggac gcggatctcg acgccgcggt caccggagcc
      661 atcgcctgca agtaccgcaa cagcggtcaa acctgcgtgt gtgccaatcg cctgctggtg
      721 caggcgccgg tgtacgaagc tttcacgcgc aagctcgtgc acgcagtcgc gcagctgcgc
      781 gtcggcgatg gactcaaggg ccccaccgat cagggaccac tgatcgatgc cggtgcgctc
      841 gaaaaggtgg agcagcacat cgccgacgcc gttggcaagg gcgcacgcgt ggcgctcggc
      901 ggccggcgcc acgcgctcgg cggcaccttc ttcgagccca ccgtggtgac cgaggtgacg
      961 tcgaccatgc gcatggctcg cgaggaaacc ttcggaccga tcgcgccgct gttccgcttc
     1021 gagaccgagc aggacgccat ccacatggcc aatgacaccg agttcggtct ggcggcctac
     1081 ttctatacgc gcgatctggc gcgtgcctgg cgcgtcgcag aagcgctcga gtacggcatg
     1141 gtcggcctca acaccggaat cttctccacc gaggtcgcgc cttttggcgg cgtcaaggag
     1201 tcgggtatcg gccgcgaagg ctccaaatac ggcatcctcg actataccga gctgaaattc
     1261 gtgtgcgtcg gcctgggttg agcaccgagc gcatcggcat cctgctggtg aactccggca
     1321 cgccggaggc gccggagccc gcggcggtgc gcgcgttcct cgcgcgcttt ctcgccgacc
     1381 ggcgcgtcgt gcagttaccg cgagcgctgt ggctgccact gctgtacggg gtgatcctcc
     1441 ccttgcgtcc gcgacgcgtc gcaccgaagt accgccgcat ctggacgcag gccgggtcgc
     1501 cgctgcgcga gctcaccgtg agattgcgtg ccgagctcgc cggtgtgctg gcgcagcgca
     1561 tgctcgcgcc gctgcaggtc gagttgggca tgctctacag cgccccgcat gttggcggcg
     1621 cgctcgagcg gctgcgtgcc ggcggggcac agcggctgct ggtgctgccg ctgtttccgc
     1681 aatattgcgg cgccaccacc ggcgcggcgt tcgaccaggt gagcgccgcg ctgcagcgcc
     1741 tgcgctggct gccggatctg cagctcatcg ccgactatca cgaccatccc ggctacatag
     1801 aagcgctgcg tgcgagcatc gtggcgcact ggaaggcgca cggccgcacg ccacacctgc
     1861 tgctgtcgtt ccatggcata cccgagcgct actgtcacca gggcgacccg tatcacaagc
     1921 gctgccacgc gactgcgcgg ctgctcgccg acgagctgct gctgcaggag ggcgagtgga
     1981 gcgtgagctt tcagtcgcgc ttcggaccgg cgcaatggct caggccgtac acgcaggcgg
     2041 tgctcggcgc gatgccggca cgcggcgtgc gggaagtcac cgtggtgtgt ccgggttttg
     2101 cggtcgactg cctggagact ctcgaggaga tcgccatcga gaaccgggcg cggttctcgc
     2161 aggccggcgg ccagcgcttc gagtacgtgc cggcgctcaa tgccgccacc gcacacgctc
     2221 agtgcctggc cgatctcgtt gcgcagcgct gtcaaagctg gacggagacg ctgcgcgcgc
     2281 acctgccggg cacggcgcac ggcgctccgg cgtgatgtcg cacgcgccgc tcccgctcgc
     2341 gggaatcttc cacgagttcg gtgtcgccgt ggccgaggta cgcagcgccg tcgagttcta
     2401 cgagcgcctc ggattcagcc aggcgaccac caccgacacc ttcagtcatc cctacggagt
     2461 gctcaccgac gggcgcttct gcatcgggct gcatcagcgc gcgggcgtat cgccgatcct
     2521 gactttcgtg cggcccggaa tcgccggcag cgtcgccgcc ctcgcggccg ccggcataca
     2581 gctgacgcgc tgccagacgg gcgaggaggt cttcaacgag atcggcttca gcgatccctt
     2641 cggacacgag ctcgccatac tcgaggcgcg cacctactcg ccggtggcgc gtgatgccgt
     2701 cgaggtctcg ctctgcgggg acttcgccga agtgagcctg ccggcggccg atttttccgt
     2761 ggcgcaaacg ttctgggagc cactcggctt cgtggcggct gagcaggcgc agtcgcccta
     2821 cccgcacctg acactcacca gcgatcacct cgacctggct ttccactcac cgcagctgtg
     2881 cgcgagcccg atgctggtgt tccacgccgc ggacatgtcg gctcgtctcg agcggctgcg
     2941 caccctcggc ttcgcgatcg cgagcgcacc ggcgcgggtg gatgccggcg ccggcaacgc
     3001 gctgctcata ggccccggga ataccccgct gctgctgctg gaagctgccg actgagggaa
     3061 gccgcggcac atgccgctgg ctgcgctagt gtctccccgg gcgcgccgcg gcgggcgccg
     3121 ccgcctgcgc gatgatgtga aagctcaccc gcatctcatc ctgcacctgc agcgcgccga
     3181 gcatggcgct gaaaggcgtg aggccgagat cggtctgacg cagggggaat tcccccgtcg
     3241 cttccagcgt gccgttgccg gcgagccggt aacgcaccgg caccgtgaag ctgcgcagct
     3301 ggccgcgcac cgagctctgc acctgggcca acaccgtggc ggaatctgcc gcgccgcctg
     3361 cgggcgcaag ctgcagtgac tgcagcacga tctccggatt gcgctgcgca tcgagcaacg
     3421 catcgctcag catgttgcga cgtgttcccg ccttcgctcc ctccgggatg tccgcgggaa
     3481 actctgccgc gggttgctgt gcccgcagcg ccgcctcatc gaccgtgagc gtggccaccg
     3541 gtatgtgcag ctcgaagctg ctctgcatga tctgctgcgg cacgtagatc gtgccgctca
     3601 ggtcgtgcga ggcgatgacg tggttgtgcc cggcgctcgc gagcgcgccg ccgcggtaga
     3661 ccagcaccgt gagcagtgac gactgcgcaa ggatcgggtg cggcgttccc aggtgcggcg
     3721 ccggcgcgga tggaacgacc gccgcggcac tgccggcggg cgcgctttgc ggcgcgggcg
     3781 gcgcaacccg ctcgggacgc gggcaaccgc agagcgccgc cagaaacagc gcggcggcga
     3841 gcgggagttt gcaacgcgca ctcacccgca taagcttgcc cgcatgtcac tgcgcaacac
     3901 cagccatcgt tggggcctgc tctcccagct gttccactgg ctcatcgtcg tcctgatcat
     3961 cctgcaggtc acgctcgcga gcctggcgga cgatctgccg cccggcgcga agaagctcgc
     4021 gctgctcgca cgacataaat ccgtcggcat caccattctc atgctggtca tcctgcgact
     4081 gctgtggcgc tcgctcaacc tcacgccgct gctgccgacg acgttgaagc cgtacgagcg
     4141 cgtgctggca cgctgcacgc acgcggcgct gtacgtgctg ctgttcgcga tgccgctctc
     4201 cggctggacg atgtcgtccg ctcgtgggtt cccggtgagc tggttcgggt tcttcacgct
     4261 gccggatctg gtgcccaaga acaaatcgct gtatgccgcg ctgctgacta cgcacgaggt
     4321 gctggcctgg gtgttggctg cggtcgtgac gctgcacgtg gctgccgcgc tcaagcatca
     4381 cttcatgctc cgcgacgatg tattgcgccg tatgctgccc ttcaccacta ccggtgaagg
     4441 accgaaagca tgatcgtcga cggcccggcg gcggcgcgcg cccgatcccg cgaccgtgcg
     4501 ctacgcgcac cggccatgtg gctcgcggcc gcgatgctca tttcccgtgc ggcggcggcc
     4561 gatgcgcccg gggtaa
//
LOCUS       DATDMP010000277         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_43363, whole genome shotgun sequence.
ACCESSION   DATDMP010000277 DATDMP010000000
VERSION     DATDMP010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43363"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VI195_04740"
     CDS             complement(<1..576)
                     /locus_tag="VI195_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823722.1"
                     /translation="MVTGGNRGIGLEICRQLAARSELRVILCSRDAAKGETAIKTIKN
                     HILNISSEVLDVSDARSIETFAQRMAKQHGRCDVLINNAGVMLDPRGSRVLDSKVETW
                     QDTLAVNLLGPLRLIQALVPLMKKNRYGRIVNVSSGQGQLSDMGSGTPAYRVSKTALN
                     ALTRTLAAELHGSGILVNAMCPGWVKTDMGGP"
     gene            complement(624..2027)
                     /gene="fumC"
                     /locus_tag="VI195_04745"
     CDS             complement(624..2027)
                     /gene="fumC"
                     /locus_tag="VI195_04745"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY6823723.1"
                     /translation="MKSKMRIERDTMGQVAVPADRLWGAQTQRSLHHFHFPGETMPLE
                     VVRAQALVKKAAALVNMELGELDRRKGAAIVKAADEALSGRHDDHFPLVVWQTGSGTQ
                     TNMNVNEVLANRASEILGGARGEKRLVHPNDDVNRGQSSNDTFPTAMHVAAMIALERN
                     LKPAVKKLRGTLDRKAKAFAKIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLAHLAT
                     AEPHLCELALGGTAVGTGLNAHPQFAEKVAAEIARLTKLPFVTAPNKFEALASCDGVV
                     HAHGALKTLAASLMKIANDVRWLASGPRSGIGELTIPENEPGSSIMPGKVNPTQSESM
                     TMACCQVLGNDVAVNIGGASGNFELNVFRPLVIRNFLHSARLLAHACENFNEHCAAGI
                     KPNRARIEQLMRDSLMLVTALNPHIGYEKAAAIAKKAHKDGTTLKAAALKLKYVTEKQ
                     FDAWVRPEKMVGKIGKA"
BASE COUNT          345 a          710 c          688 g          434 t
ORIGIN      
        1 cgggccgccc atgtcggtct tcacccagcc cgggcacatc gcgttcacca ggatgccgct
       61 gccgtggagc tcggccgcga gcgtgcgggt cagggcattc agcgcggttt tcgacacgcg
      121 gtaggccggc gtcccggaac ccatgtcgga gagctgcccc tgcccgctcg acacgttgac
      181 gatgcgaccg tagcggttct tcttcatgag cggcacgagc gcctggatga ggcgaagcgg
      241 gccgagcagg ttgaccgcga gcgtgtcctg ccaggtctcc actttcgagt cgaggacgcg
      301 cgagccgcgt ggatcgagca tcactccagc attgttgatg aggacgtcgc agcgcccgtg
      361 ctgcttcgcc atgcgttgcg cgaaggtctc gatgctgcgc gcgtcgctga catccagaac
      421 ctcggatgat atattaagta tatgattttt aatagttttt atagctgtct cgcccttggc
      481 cgcatcgcgg ctgcaaagga tcacgcgtag ctcgctgcgc gccgcgagct ggcggcagat
      541 ctcgagaccg atgccgcgat ttccgccggt gaccaccgcg acgcgttgga ggatggtttt
      601 cgtcaatcgt cacccgtcac gcgtcatgcc ttgccgatct tcccgaccat cttctccggt
      661 cgcacccacg catcgaactg cttctcggtc acgtacttga gcttcagcgc cgcggccttg
      721 agcgtcgtgc cgtccttgtg cgccttcttg gcgatggcag cggccttctc gtagccgatg
      781 tgcggattca gcgccgtcac gagcatgagc gagtcgcgca tcagctgctc gatgcgcgcg
      841 cgattgggct tgatgcccgc ggcgcaatgc tcgttgaaat tctcgcaggc atgcgcgagc
      901 agcctggcgc tgtgcaggaa attgcggatg acgagcggcc ggaacacgtt cagctcgaaa
      961 ttgcccgatg cgccgccgat gttgaccgcg acgtcgtttc cgagcacctg gcagcacgcc
     1021 atggtcatcg actccgactg cgtgggattc accttgcccg gcatgatcga actgcccggc
     1081 tcgttctccg ggatcgtcag ctcgccgatg ccgcttcgcg gcccggaagc gagccagcgt
     1141 acgtcgttcg caatcttcat caggctcgcg gcgagcgtct tgagagctcc gtgcgcgtgc
     1201 accacgccgt cgcaggacgc aagcgcctcg aacttgttcg gggcggtcac gaacggcagc
     1261 ttcgtcagcc gcgcaatctc cgccgcgact ttctccgcga actgcgggtg ggcgttcaac
     1321 cccgtgccga ccgcggttcc gcccagcgcg agctcgcaca ggtgcggctc cgcggtcgcg
     1381 aggtgggcga gcccgtggtc gagctgcgat acgtagcccg agaattcctg ccccagcgtg
     1441 agcggcgtcg cgtcctgcag gtgggtacgt ccgatcttca cgatctttgc aaatgctttc
     1501 gccttgcggt cgagcgtgcc gcgcagcttc ttcaccgcgg gtttcagatt gcgttcgagc
     1561 gcgatcatgg ccgcgacgtg catcgcggtg ggaaacgtgt cgttggagga ctgcccgcgg
     1621 ttgacgtcgt cgttcggatg caccagccgc ttctcgccgc gcgcgccgcc caggatttcc
     1681 gaagcgcggt tggcgagcac ctcgttcacg ttcatgttgg tctgggtgcc cgagccggtc
     1741 tgccacacca ccagggggaa atgatcatcg tgccggccgg aaagcgcttc gtccgccgcc
     1801 ttcacgatcg cggcgccctt cctgcggtcg agctcgccaa gctccatatt gaccagggcg
     1861 gcggccttct tgaccagcgc ctgcgcacgc accacctcga gcggcatcgt ctcgccggga
     1921 aagtgaaaat ggtggaggct gcgctgggtc tgcgcacccc acaggcggtc ggccggcacc
     1981 gccacctgcc ccatcgtgtc gcgctcgatt cgcattttgc ttttcatgct caacctctca
     2041 acgcagagga cgcagtggac gcgggggaaa gcaactcgaa ttcgatagtg taagcctctc
     2101 aatgcagagg acgcagtgga cgcagcggcg tgcggaaaca ggaatcgaag aacctattct
     2161 cttcagattt tcctccg
//
LOCUS       DATDMP010000278         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_932427, whole genome shotgun sequence.
ACCESSION   DATDMP010000278 DATDMP010000000
VERSION     DATDMP010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932427"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VI195_04750"
     CDS             <1..864
                     /locus_tag="VI195_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY6823724.1"
                     /translation="LACLVLVCGFVLRRWRWRAFAAFLVLFVLLLAAWNTIAPSNAGE
                     WEPENAVLAYATIDGEHITLHNLRNFDYRTETDFTPSYYDRAFDLHQLEEVDLYSVYW
                     MGPAIAHVFLSFGFRDGDHVAFSIETRREKGQSYSSVAGFFRQFPLYYAVGDERDIVR
                     VRTNYRHNPPERVYLYRLRGSNAVAKQLFLEYLGRINELKQRAEWYNTITSNCTTSIW
                     VMTRLNPGHVPFSWKILLSGHLPEYLYEQGRLDTSVPFAELQRRALINPLAQAADQAA
                     DFSQRIRAGVP"
     gene            complement(1020..1322)
                     /locus_tag="VI195_04755"
     CDS             complement(1020..1322)
                     /locus_tag="VI195_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823725.1"
                     /translation="MLPPIPHTSSPAAPRLRIVPEHRGQQEGFGALYKGIGTVLGLIG
                     ACGAFLAVYVAAIELNGWFIGLALALATAGFAAAAAFFALRYLWPCLALLLIPFLH"
     gene            1533..>2084
                     /locus_tag="VI195_04760"
     CDS             1533..>2084
                     /locus_tag="VI195_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823726.1"
                     /translation="MGKRARLLIGGCQGILAALPIASDAGISYVQGAAEVSARREIEA
                     SATYPSTQVAGDLDVIFIAWASAAAEVVSVSDTSGNLYLRVGSISNAGVATQVTYYAN
                     HIARAAPGRNTVTVRFSSPIQQATLGITEYHGVDPQKPIAAAAGAAGISMTAASGSLV
                     IPDPGAVLVSSAFTAGQALGPGPF"
BASE COUNT          389 a          648 c          671 g          376 t
ORIGIN      
        1 cttgcctgtc tggtgctcgt ctgcggattc gtgctgcggc gctggcgctg gcgggcgttc
       61 gccgctttct tggtgttatt cgtgctgttg ctcgccgcct ggaacaccat cgcgccatcc
      121 aacgccggcg aatgggagcc cgagaatgcg gtgctcgcct acgcgacgat cgacggcgag
      181 cacatcacgc tgcacaacct ccggaacttc gactaccgta ccgagaccga ctttactccg
      241 tcgtactacg accgcgcttt cgacctgcac cagctcgagg aggtggatct ctattcggtc
      301 tactggatgg gtccggcgat cgcgcacgtg ttcctgagct ttggcttccg tgacggtgat
      361 cacgtggcgt tctcaattga aacccgccgc gagaaaggcc agagctactc gtcggtagcg
      421 gggttcttcc gccagtttcc cctctactac gccgtgggtg acgagcgcga catagtgcgg
      481 gtgcgcacca actaccggca caatcctcca gagcgtgtgt atctgtaccg cctccgtggc
      541 agtaacgcgg tagccaagca actgttcctc gaatacctgg gccggatcaa cgagctcaag
      601 caacgtgccg agtggtacaa caccatcacc agcaactgca ccacgagtat ttgggtcatg
      661 acacgcctca accccgggca tgtgcccttt tcgtggaaga tcctgctgag cggccatttg
      721 ccggagtatc tctacgagca gggcaggctc gacaccagtg tgcccttcgc ggagctgcag
      781 cggcgcgcgc tcatcaatcc gctggcgcag gccgccgacc aggcggcgga cttctcgcag
      841 cgcatccgtg ccggcgtgcc ctagggagac agcgaacagg aagaacgcgg ccttcgatcc
      901 gtcaccgcag atcggtcagt tcaagggtgg gcgggttggc gagttctacg ggcaggacac
      961 actcgacggt aaagacgcgt tgagcctgcc gtccgagatg ggcgcacccg gcgtgcgcgc
     1021 taatgcagaa atgggatcag cagcagcgcc agacagggcc agaggtaacg cagagcaaag
     1081 aacgcggccg cagccgcaaa ccctgcagtg gccaacgcca gtgccaggcc gatgaaccag
     1141 ccgttgagct cgatcgcagc tacatacacg gcgaggaagg cgccgcaggc tccgatcagg
     1201 ccgagaacgg tgccgatgcc cttatagagc gcgccgaagc cctcttgctg gccacggtgt
     1261 tcgggcacga tccgcagacg gggggcggcg ggagaggatg tgtgggggat gggtggcagc
     1321 atggtagttc gcaggaccag gcctggcgtg tgcggttgag acgcaaatgg agccgtcgat
     1381 cggctcacgt taagggatga aatgggcctc gcgatgtgcc ctgtttgtta ttttcagctc
     1441 taaactaaca gactgtgcgc cagtacgcca gtgctggaca taaggcgtac agcgctcgcc
     1501 cgaaccaccc ccaacctcac tggatgcgtc aaatggggaa gcgtgcccgg cttctcatcg
     1561 ggggctgcca ggggattctg gcagctctcc cgatagcgtc cgatgcaggc atctcgtacg
     1621 tgcagggcgc cgccgaggtc tcggcacggc gtgaaatcga ggcctcggcg acctatccga
     1681 gcacgcaggt ggcgggggat ctcgacgtca tattcattgc ctgggcgagt gccgctgccg
     1741 aggtcgtatc ggtctccgac acgagcggta acctctacct gcgggttggc tcgatatcga
     1801 atgcgggcgt agcgacgcag gtcacctact atgcgaacca catcgcgcgg gctgcgccag
     1861 gacgcaatac agtcacggtg cgcttcagta gccccatcca acaggccacc ctcgggatta
     1921 ccgagtacca cggcgtcgat ccgcagaagc cgatcgctgc cgcggctggc gcggcgggca
     1981 tctccatgac cgccgctagc gggtcgctgg tcatcccgga tccaggggca gtgctggttt
     2041 cgagtgcctt cacagccggg caggctctcg gaccgggacc gttt
//
LOCUS       DATDMP010000279         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2154791, whole genome shotgun sequence.
ACCESSION   DATDMP010000279 DATDMP010000000
VERSION     DATDMP010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154791"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VI195_04765"
     CDS             <1..529
                     /locus_tag="VI195_04765"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY6823727.1"
                     /translation="AEIAATIRTIADYPKAGVTFRDITTLLGNPRAFASAVEQLARPW
                     DGAAVDRVAGIEARGFILGGAIAQRLAAGFVPIRKKGKLPHETVRVAYSLEYGIDEME
                     MHRDAVRSGEGIVLVDDLIATGGTACAAVQLLRSLGTRVLAACFIVDLPDLGGSARLA
                     SLGVPVRTVVAFPGH"
     gene            704..904
                     /locus_tag="VI195_04770"
     CDS             704..904
                     /locus_tag="VI195_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823728.1"
                     /translation="MFKKLIPVIALALIAGPAFAQSTESKESKETPAATATPAKSHHK
                     KHHKKESKEKKSSSESSSAPPK"
     gene            1081..1302
                     /locus_tag="VI195_04775"
     CDS             1081..1302
                     /locus_tag="VI195_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823729.1"
                     /translation="MTFPSCDQEDLIEAIDRVEEQLHTTHPNLALMCRFLNSIARSLR
                     AQPEARDACLAIEDAIGKAGMPSTWQSGI"
     gene            1355..>2530
                     /locus_tag="VI195_04780"
     CDS             1355..>2530
                     /locus_tag="VI195_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6823730.1"
                     /translation="MIAPLTQVLVLLAVSVFLVTVARRLSQPTTQAYLVVGLLLGPHA
                     LGVVSDSGTTRLLAELGVAFLLFTLGLEFSLPRMLAMRREVFGLGALQVAVTAAVFAL
                     LAHLPGIPWLPAIVLGGAVAMSSTAILLQQLTERAELNRTHGRLAFSMLLFQDLAFVP
                     FLALSAALAAGGSRFELRGSIEAAIGAVLAVVAVLAAGRWLLRPLFHEIAHSRLRELF
                     TLAVLLVVLSSAWVSHLAGLSFALGAFLSGMMLAETEYRHQVESVIRPFRDILLGLFF
                     ISVGMLLDLRLLAGLSGLALVSLLLVLLVVLKAIIAALVTRPFTSSRFRALRTGIVIA
                     IGGEFGVALCTIMLQAGLVPAELGQPLLVAIVLGGAVAMSSTAILLQQLTERAELNRT
                     "
BASE COUNT          346 a          889 c          847 g          448 t
ORIGIN      
        1 cgccgagatc gccgcgacga tccgcaccat cgccgattac ccgaaggccg gcgtcacctt
       61 ccgcgacatc accacgctgc tcggcaaccc gcgcgccttc gcgagcgcgg tcgagcagct
      121 ggcgcggccc tgggacggcg ccgcggtcga ccgggttgcg ggcatcgagg cccgcggttt
      181 catcctcggc ggcgcgattg cccagcggct ggccgcgggt ttcgtgccca tccgcaagaa
      241 gggcaaactc ccgcacgaga ccgtgcgggt ggcctactcg ctcgagtacg ggatcgacga
      301 gatggagatg caccgcgacg cggtccgcag cggcgaagga atcgtgctcg tcgacgacct
      361 gatcgccacc gggggtaccg cctgcgcggc ggtgcagctg ctgcgcagcc tcggcacccg
      421 ggtgctggcg gcgtgtttca tcgtcgatct gccggacctt ggaggctccg cccggctcgc
      481 ctcgctcggg gtgcccgtgc gcaccgttgt tgcctttccc ggacactgaa agcggcggtt
      541 tagcgagcag tcaggttggc tcgttctcct tatctgtcaa gcggatacgc gcgcttccgc
      601 cgcaacatta catcgcggtc atatggcgct tgcgcgacgg gaatccagtg ataatacgcg
      661 cgctttggtt gttcgcgcac tcacctttct ggagagcaca tcgatgttca agaagctgat
      721 ccccgtgatt gccctggccc tgattgccgg cccggccttc gcccagtcga ccgagtcgaa
      781 agagtccaag gagaccccgg ctgccacggc gaccccggcg aagtctcatc ataagaagca
      841 ccacaagaaa gagtccaaag agaagaagag ctcgagcgag tcctcgagcg cgccgcccaa
      901 gtaagacggt cggtgtcgag tccggggagc cccgccttca gaggcggggc tcctcgcttt
      961 cgggagcgcc gcagggcttg cggcccggcg caaaccggtt caaatttcgc tcccatggat
     1021 gggacgcaac aggccgcaat acaccaggcc ctggtctcag tacaacatgc cgtcacgagc
     1081 atgactttcc cgagctgtga ccaggaagat ctgatcgaag ccatcgatcg cgtcgaagag
     1141 cagctgcaca ccactcaccc gaacctggcg ctcatgtgcc gcttcctcaa ctcgatcgcc
     1201 cgcagcctgc gcgcgcagcc ggaagcgcgt gacgcctgtc tggcgatcga agatgcgatc
     1261 ggcaaggccg gcatgccgag cacctggcag tcggggatct gaccggggct cacttggcgg
     1321 cgccgcgccc cacgcggtct gagctggggc accgttgatc gcgcctctca cgcaggtcct
     1381 ggtactgctc gccgtctcgg tcttcctggt gacggtggcg cggcggctgt cgcagccgac
     1441 cacccaggcc tatctcgtgg tcgggctgct gctcgggccc cacgcactcg gcgtggtgtc
     1501 ggattcgggc accacgcggc tgctggccga gctcggcgtc gccttcctgc tgttcacgct
     1561 cgggctcgag ttctccctgc cgcgcatgct cgccatgcgc cgcgaggtat tcggtctcgg
     1621 ggcgctgcag gtcgcggtca ccgccgcggt gttcgcgctg ctcgcgcacc tgcccggcat
     1681 cccctggctg cccgcgatcg tgctcggcgg cgcggtcgcg atgagctcga ccgcgatcct
     1741 gctgcagcag ctcaccgagc gcgccgagct caaccgcacc cacgggcggc tcgccttcag
     1801 catgctgctg ttccaggacc tggcgttcgt gccgttcctg gcgctcagcg cggcgctcgc
     1861 cgccggcggg agccgtttcg agctgcgcgg cagcatcgag gccgcgatcg gcgccgtgct
     1921 ggcggtggtg gcggtgctgg cggccggacg ctggctgctg cggccgctgt ttcacgagat
     1981 cgcccacagc cgcctgcgcg agctgttcac gctcgcggtg ctgctggtgg tgctctcctc
     2041 ggcctgggtg tcgcacctcg ccggactgtc gttcgccctc ggcgcgttcc tctccggcat
     2101 gatgctggcg gagaccgagt accgccacca ggtcgaatcg gtcatccggc cgttccgcga
     2161 catcctgctc ggcctgttct tcatctcggt cggcatgctg ctcgatctgc gcctgctcgc
     2221 cgggctgagc gggctggcgc tggtgtcgct gctgctggtg ctgctcgtgg tcctcaaggc
     2281 catcatcgcc gcgctggtca cgcgtccgtt caccagctcg cgcttcaggg cgctgcgcac
     2341 cgggatcgtc atcgcgatcg gcggcgaatt cggcgtggcg ctgtgcacca tcatgctgca
     2401 ggcgggcctg gtgccggccg agctcgggca gccgctgctg gtggcgatcg tgctcggcgg
     2461 cgcggtcgcg atgagctcga ccgcgatcct gctgcagcag ctcaccgagc gcgccgagct
     2521 caaccgcacc
//
LOCUS       DATDMP010000280         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1599117, whole genome shotgun sequence.
ACCESSION   DATDMP010000280 DATDMP010000000
VERSION     DATDMP010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599117"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VI195_04785"
     CDS             <1..399
                     /locus_tag="VI195_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6823731.1"
                     /translation="EVLSPDNEPAHWDVIEGQGSLYHPAYAAVTLGLLHGSQPDAIVL
                     CHEPTRTTIEEYPDYPIPPLGRVIDAYVRAGRLTNHAIRCIGISINSSELSETDWQGY
                     RVGLERELGLPVVDPMRSGVATVAEALTKL"
     gene            396..1379
                     /locus_tag="VI195_04790"
     CDS             396..1379
                     /locus_tag="VI195_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEY6823732.1"
                     /translation="MIALSTRLHAWELREPFATARDCLTQLRVLLLELTGKDGARGRA
                     EAAGVDYDGENPSGMAAQLASIEAQLHDGISGDDLLELLPAGGARNALDCALWDLRAK
                     ESGVPAASSAGLEALRPVTTAFTIGLGSDADTRRRARAAAAYPILKLKLDAERHIDIV
                     RLVREERPSARLLVDANQSWTRALLEKLLPQLQALGVELVEQPVARGADDSLEGLASA
                     IPLAADESCTDRSSLSALAGRYQYVNIKLDKCGGLTEALALSVEATRQGLGLMVGNMC
                     GSSLAMAPAFLIAQRCRYVDLDGALLQRLDYSPAIRCEHGLMYPPSRALWG"
     gene            complement(1384..2220)
                     /locus_tag="VI195_04795"
     CDS             complement(1384..2220)
                     /locus_tag="VI195_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823733.1"
                     /translation="MSATMQIQRTLVLLFAAACANTFATDAASDSSLAAAIASSARTP
                     KYAARDAYRHPLQTLQFFGLRPDQTVVEIWPGRGWYMEILAPYLRDRGKYYAAIEAPD
                     VSGASQEAKDDASFLRKRITDDPAHFGKVILTSLHPPQLTEICPPGTADLVLTFRNVH
                     NWIDDGTQQQQFNAFFKALKPGGVLGVVEHRAKPGTSLDETRKSGYVDEAYVKKLATA
                     AGFRFVGASNVNDNPKDTKDYPKGVWTLPPTLTMGVQDRDKYLAIGESDRMTLKFMKP
                     NK"
BASE COUNT          408 a          811 c          812 g          474 t
ORIGIN      
        1 gaggtgctct cgccggataa tgagcccgct cattgggatg tcatcgaggg gcagggctct
       61 ttgtatcacc cggcttatgc ggccgtgacg ctcggactcc tgcacgggtc ccagcctgac
      121 gccatcgtgc tgtgtcacga acccacgcga accacgatcg aggagtaccc ggactatccc
      181 attccgccgc tcggcagggt gatcgacgca tacgtacgtg cggggcgttt gaccaaccac
      241 gcaatccgct gtatcggcat cagcatcaac tccagcgagc tctccgaaac cgactggcag
      301 gggtatcggg tgggcctcga gcgagagctc ggtctgccgg tcgtcgatcc gatgcgcagt
      361 ggcgtcgcaa ccgttgcgga ggcgctgacc aaactgtgat tgccctcagc acgcgccttc
      421 atgcctggga gctgcgcgag cctttcgcga ctgcccgcga ctgcctcact cagctgcggg
      481 tgctgcttct cgagctgacg ggcaaggatg gcgcacgcgg ccgggcggag gctgccggcg
      541 tcgattacga cggtgagaat cccagcggca tggcggcgca gctcgcgagc attgaggcgc
      601 agctgcacga tggcatcagc ggcgacgatc tgctcgaact tctgcccgcc ggcggtgcgc
      661 gcaacgcgct cgactgtgcg ctgtgggacc tgcgcgccaa ggagagcgga gtcccggcgg
      721 cctcctcggc cgggctcgag gcgctgcgtc ccgttaccac ggcgttcacc atcgggctcg
      781 gcagcgacgc cgatactcgt cgccgagcac gcgcggctgc ggcgtatccc atcctcaagt
      841 tgaaactcga cgccgagcgc cacatcgaca tcgtccgcct ggtgcgcgaa gagcgaccct
      901 cggcgcggct gctggtcgat gccaatcaat cctggacccg agctttgctt gagaagctcc
      961 tgccgcagct ccaggctctc ggtgtcgagc tggtcgagca acccgtggca cgcggcgcgg
     1021 acgactcact cgagggcttg gcgagcgcaa tacccctcgc cgccgatgag tcctgcacgg
     1081 atcgcagctc gctcagtgca ctcgccggtc gttaccagta cgtcaacatc aagctcgaca
     1141 agtgcggtgg cctcaccgag gccctggcat tgtccgtcga ggccacgcgg cagggcctcg
     1201 gactcatggt cggcaacatg tgcggcagct cgctcgcgat ggcgccggca tttctcatag
     1261 cgcagcgctg ccgctacgtc gatctcgatg gagcgctctt gcagcggctc gattactctc
     1321 cggcgatccg ctgcgagcac ggcctcatgt atccaccctc ccgcgctctg tggggttgag
     1381 ggcctatttg ttcggcttca tgaacttcag cgtcatccga tcggactcgc cgatggccag
     1441 gtatttgtcg cgatcctgaa cgcccattgt gagggtgggc ggcagtgtcc acaccccctt
     1501 cgggtaatcc ttggtgtcct tgggattgtc attgacgttc gatgcgccaa cgaagcgaaa
     1561 gccggcggcg gtggcgagct tcttgacata ggcctcatcg acgtagccgg acttgcgcgt
     1621 ctcatcgagg ctggttcccg gtttggcgcg gtgttccacc accccgagca caccgcccgg
     1681 tttcagcgcc ttgaagaagg cattgaactg ctgctgctgc gtgccatcgt cgatccagtt
     1741 atgcacgttg cggaaggtga gcacgagatc cgcagttccc ggcgggcaga tctcggtgag
     1801 ctgcggcgga tgtaggctcg tcaggatgac cttgccgaag tgtgccgggt cgtcagtgat
     1861 gcgcttgcgc aggaagcttg cgtcgtcctt cgcctcttgc gacgcgccgc tgacatcagg
     1921 cgcctcgatg gctgcgtagt acttgccccg gtcacgcagg tagggcgcga gtatctccat
     1981 gtaccagccg cggcccggcc agatctccac caccgtctgg tccggccgca gcccgaaaaa
     2041 ttgcagcgtc tgcaaaggat gacggtaggc atcgcgcgcg gcgtacttcg gcgtgcgcgc
     2101 gctgctggcg atcgccgcag ccagtgacga atcggaagcg gcgtcagtgg cgaaggtatt
     2161 tgcgcaggcg gcagcgaaca acagtaccag cgtgcgctgg atttgcatcg tggcagacac
     2221 ggcggcagtt cccctgtggc tgagtgaaag cccgaatact accccagcgc aggccgaccc
     2281 ggtcgatcta ctcagctacc tgcgcccggg tgccctcgat tctcgtatgc taccgatccg
     2341 gtagcggcgc agggcagagt agcttgagac gccttcacaa cattgccgtc cggcacacgc
     2401 gactgtggtt ggggacgatc gtcgcagtcc tgatcttcct tatctcgccc cccgaatggt
     2461 cgctgctggc gcgcgtgctg agcgcctgga acgggggagt gttgt
//
LOCUS       DATDMP010000281         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_933218, whole genome shotgun sequence.
ACCESSION   DATDMP010000281 DATDMP010000000
VERSION     DATDMP010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933218"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VI195_04800"
     CDS             complement(<1..1125)
                     /locus_tag="VI195_04800"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY6823734.1"
                     /translation="MTRFVFVTGGVVSSLGKGIAAASLGAILEARGLRMAMVKLDPYI
                     NVDPGTMSPFQHGEVFVTQDGTETDLDLGHYERFVRTTTGRNSNFTTGRIYERVIAKE
                     RRGDYLGATVQVIPHITDEIKRCIKLGADGADVCMVEIGGTVGDIESLPFLEAIRQLG
                     VELGRNNCVYMHLTLVPVVGGSGEIKTKPTQHSVKELRGIGIQPDVLLCRCKHPLPEE
                     QRRKIALFTNVEERAVISAVDADDIYRIPILLHEQGLDEIVVDKLRLEVPPTDLAEWR
                     HVVSGRANPDGQVDIAMVGKYVQIRDSYLSLHEALMHAGLKSRTRVNIHYIESTDIEQ
                     HGTHALKDVDAVLVPGGFGERGIEGKIQAVRYAREHGVPYL"
     gene            complement(1351..>2003)
                     /gene="tilS"
                     /locus_tag="VI195_04805"
     CDS             complement(1351..>2003)
                     /gene="tilS"
                     /locus_tag="VI195_04805"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY6823735.1"
                     /translation="DADGASVGAMLSAKMLRRLTPERRRNALRFWISAAGARTPPAAR
                     LSEIAGALLEARADAHPWVTWAGAALEREGDLLRLHRAPPAKAAAPQPLAAAAPDLTW
                     HWRTKHTQVLPAPFGTLMLKRDTRGPLDLDALGPTLVLCGRRGGERLRPVRGGPRRAL
                     KQLLQEARVPAARRAQLPLLFDRGRLVGVADLWLDESVQAHAASRRRARLLWRTAE"
BASE COUNT          319 a          683 c          677 g          324 t
ORIGIN      
        1 gagatacggc accccatgct cgcgggcata gcgcacggcc tggatcttgc cctcgatgcc
       61 gcgctcgccg aagccgccgg gaaccagcac agcgtccaca tccttcagcg catgcgtgcc
      121 gtgctgctcg atgtcggtcg attcgatgta gtggatgttg acgcgtgtgc ggctcttcag
      181 accggcgtgc atcagcgcct cgtgcagcga cagataggag tcgcggatct gcacgtactt
      241 gccgaccatg gcgatatcga cctggccgtc gggattggcc ctgccgctca ccacgtggcg
      301 ccattcggcg agatcggtgg gtggcacctc caggcgcagc ttgtcgacca cgatctcatc
      361 gagcccctgc tcgtgcagca ggatcgggat gcgatagatg tcgtccgcat cgaccgcgga
      421 gatcaccgcg cgctcctcga cgttggtgaa cagggcgatc ttgcggcgct gctcttcggg
      481 cagtggatgc ttgcagcggc acagcagcac atccggctgg atgccgatcc cgcgcagctc
      541 cttcaccgaa tgctgcgtcg gcttggtctt gatctccccg gagccgccca ccaccggcac
      601 cagcgtgaga tgcatgtaga cgcagttgtt gcgcccgagc tccacgccga gctggcgaat
      661 cgcctcgagg aaggggagcg actcgatgtc gcccaccgtg ccgccgatct cgaccatgca
      721 cacatcggca ccgtcggcgc cgagcttgat acagcgcttg atctcgtcgg tgatgtgcgg
      781 gatcacctgc acggtggcgc cgaggtagtc gccgcgccgt tccttggcga tgacgcgctc
      841 atagatccgc ccggtggtga agttgctgtt gcgcccggtg gtggtgcgta cgaagcgttc
      901 gtagtggccg agatcgaggt cggtctcggt gccgtcctgg gtgacgaaca cctctccgtg
      961 ctggaaaggg ctcatggtgc ccgggtcgac gttgatgtag gggtcgagct tcaccatggc
     1021 catcctgagc ccgcgcgcct cgaggatcgc accgagcgag gcggcagcga ttcccttgcc
     1081 caatgaggac acgacgccgc cggtgacgaa gacaaaacgc gtcataagaa gctcgaggag
     1141 ttcccgtcgt atgaggtcgc tgcccgaaat gtgcggggcg cgacgacggg caagtagagt
     1201 agcacaactc actccggccc gctcgggcta gcggttttca catcgagcct ggagcacagg
     1261 cgccggtggc gatagttcac caattggtga actattgcct gtgacacctg caacagcgcg
     1321 agtgagccgc cctggcccgc acgccgcgcc tcactcagcg gttcgccaga gaagtcgcgc
     1381 gcggcgccgg ctcgcagcgt gcgcctgcac cgattcgtcg agccacaggt ccgcgacgcc
     1441 aacgagccga ccgcgatcga acaacagcgg cagctgcgca cggcgcgccg cgggcacgcg
     1501 cgcttcctgc agcagctgct tgagcgctcg ccgcggacca ccgcgcaccg ggcgcaggcg
     1561 ctcgccgccg cgacgtccgc acagcaccag cgtgggccct agcgcatcga ggtcgagcgg
     1621 cccgcgcgtg tcgcgcttga gcatgagcgt gccgaagggt gccggcagta cctgggtgtg
     1681 cttggtgcgc cagtgccagg tgagatcggg cgcagccgcc gcgagcggct gcggtgcggc
     1741 ggccttcgcc gggggcgctc ggtgcaacct cagcagatcg ccctcgcgct ctagggcggc
     1801 gcccgcccag gtgacccacg ggtgagcgtc cgcgcgcgcc tcgagcagcg caccggcgat
     1861 ctcactcagt cgagcggccg gcggagtacg cgcccccgcg gcgctgatcc agaagcgcag
     1921 cgcattgcgc cgccgctcag gcgtcagccg ccgcagcatc ttcgccgaga gcatcgctcc
     1981 gacactcgcg ccgtccgcat ccg
//
LOCUS       DATDMP010000282         2723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1043804, whole genome shotgun sequence.
ACCESSION   DATDMP010000282 DATDMP010000000
VERSION     DATDMP010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043804"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VI195_04810"
     CDS             <1..1134
                     /locus_tag="VI195_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY6823736.1"
                     /translation="HIEANGGGKSVAVRFRKDGVLVHYLDIPARFRGAVTSRLKIMSQ
                     LDIAERRKPQDGKIAFKRFGPSDVELRIATIPTSNGLEDVVMRVLSAATAKPLDTLGL
                     DSQSLEKVRGLIARPHGLLLVCGPTGSGKTTTLHSVLGALNTSEKKIWTAEDPIEIAQ
                     PGLRQVQVNPKIGWTFAAALRSLMRADPDIIMVGEMRDAETAKMGIEASLTGHLVLST
                     LHTNSAAESVIRLLDLGMDPFNFADALLGVLAQRLVRTLCTHCRTRLEPSAREVTALA
                     AEYCGDTGLDVQEVVKKWRQDKITLYAAKGCKECDRTGYRGRTAVWELMVADKAVKRL
                     VQKRAPVEEIVEAARAGGMRTLKQDGIEKVLHGVTDMAQVRAL"
     gene            1414..1599
                     /locus_tag="VI195_04815"
     CDS             1414..1599
                     /locus_tag="VI195_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823737.1"
                     /translation="MGKAPAPEPGRPIIVLAGGHHGELLVEGKIDPEVYERWNRAAGD
                     QKLPEHVRKKKRKKRKR"
     gene            1670..2107
                     /locus_tag="VI195_04820"
     CDS             1670..2107
                     /locus_tag="VI195_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6823738.1"
                     /translation="MRFMVMVKATKDSEAGKMPSQELLAAMGKYNEELVKAGIMLSGE
                     GLQPSRKGARVRFVGDKRIVVDGPFTETKELVAGFWMWQIKSKEEAIEWVKRCPNPMP
                     GSEAEIEIRQVFEAEDFGEEFTPELREQEERVRAQAARQAQKR"
     gene            2183..2551
                     /locus_tag="VI195_04825"
     CDS             2183..2551
                     /locus_tag="VI195_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6823739.1"
                     /translation="MNANPVGWFEIYVQDMERAKRFYESVFQGKLQKLNSPGMDMWAF
                     PMEMNGAGAAGALVKLQGVPSGGSTLVYFRCDDCATEQRRATQAGGKIHKEKFSIGEY
                     GFISLVVDPEGNMLGLHSMK"
BASE COUNT          540 a          839 c          933 g          411 t
ORIGIN      
        1 cacatcgagg ccaatggcgg cggcaagagc gtggccgtgc gcttccgcaa ggacggcgtg
       61 ctcgtccact acctcgacat tcccgcgcgc ttccgcggcg cggtcacctc gcggctgaag
      121 atcatgagcc agctcgacat cgccgagcgg cgcaagccgc aggacggcaa gatcgcgttc
      181 aagcgcttcg gtccttccga cgtcgagctg cgcatcgcga ccattccgac ctcgaatggc
      241 ctcgaggacg tggtgatgcg cgtgctctcc gcggccactg ccaagccgct cgacacgctg
      301 ggactcgact cccagtcgct ggagaaagtg cgcggcctga tcgcgcggcc gcatggattg
      361 ctgctggtct gcgggccgac cggatcgggc aagaccacca ccctgcattc ggtgctcggc
      421 gcgctcaaca ccagcgagaa gaagatctgg accgccgagg acccgatcga gatcgcgcag
      481 ccgggattgc gtcaggtgca ggtgaatccc aagatcggct ggaccttcgc cgccgcgctg
      541 cgcagcctga tgcgcgcgga ccccgacatc atcatggtcg gcgagatgcg cgatgcggag
      601 acggcgaaga tgggcatcga ggcttcgctg accggccacc tcgtgctctc cacgctccat
      661 accaacagcg cagcggagag cgtaatccgc ctgctcgacc tcggcatgga tcccttcaac
      721 ttcgccgacg cgctgctcgg cgtcctggcg cagcgcctcg ttcgaacgct gtgcacgcat
      781 tgccgcacgc gtctcgaacc gagcgcacgg gaagtaacgg cgctcgccgc cgaatactgc
      841 ggcgacaccg gactggacgt gcaggaagtg gtcaagaaat ggcgccagga caagatcacc
      901 ctttatgctg ccaagggctg caaggaatgc gaccgcaccg gctaccgcgg ccgcaccgcc
      961 gtctgggagc tcatggtggc cgacaaggcg gtgaagcgcc tggtgcagaa gcgcgcgccg
     1021 gtggaagaga tcgtggaagc cgcgcgggca ggcggcatgc gcaccctcaa gcaggacggc
     1081 atcgagaagg tgctgcacgg cgttaccgat atggcgcagg tgcgcgcctt atgagccgga
     1141 cggatagtcc gatatatcag atgtgaaacc gcgctaccgc acgtctgccg cggcgaaata
     1201 tccttagccc agccaccaca aaaatcagcg gaataccgca acggaggagg gcatgaacga
     1261 cgtgcaaacc gtggcgcagc tgctcgagcg cgcggtgcag gtcctgcggg ttgctgcgcg
     1321 ggacgcccgc gcaggttgta ccgcgccagc tattctggaa tcggtcaagg cggggttcgg
     1381 ggacctcgag gccgcgaaga caatcctgga gaagtgggca aagcgcccgc gcccgaaccc
     1441 ggacggccga taatagtcct cgcaggagga catcatggcg agctgctggt cgagggcaag
     1501 atcgaccccg aggtgtacga gcgctggaat cgtgccgcgg gcgaccagaa gctgcccgag
     1561 cacgtccgca agaaaaagcg caagaagcgc aaacgctgac ccgggatgtc gatttccggc
     1621 cttgccgttc gactacaagg caagcgaccc cactttccag gaggcagaca tgagattcat
     1681 ggtcatggtg aaggccacca aggattcgga agccggcaag atgccgagcc aggagctcct
     1741 ggcggccatg ggcaagtaca acgaggagct ggtcaaggcc ggcatcatgc tgagcggcga
     1801 aggcctgcag ccgagccgca agggcgcgcg ggtgcgcttc gtgggtgaca agcggatcgt
     1861 cgtggacggc ccgttcaccg agaccaagga gctggtcgcc ggcttctgga tgtggcagat
     1921 caagtcgaag gaagaggcga tcgagtgggt gaagcgctgt ccgaatccga tgcccggcag
     1981 cgaggccgag atcgagatcc gccaggtgtt cgaggccgag gacttcggcg aggaattcac
     2041 gcccgagctg cgcgagcagg aagagcgcgt gcgcgcgcag gcggccaggc aggcgcagaa
     2101 acgctaaggc atggggtcag gtcttgcgtt gacggtgatt ctgcgtcaat gcaagacctg
     2161 accccgatca ttggaggtgc aaatgaacgc gaatccggta ggctggttcg agatctacgt
     2221 gcaggacatg gagcgcgcca agcgcttcta cgagtcggtg ttccagggca agctgcagaa
     2281 gctgaactcg cccggtatgg acatgtgggc gttcccgatg gaaatgaacg gcgccggcgc
     2341 ggcgggcgcg ctggtgaagc tgcaaggcgt gccctcgggc ggcagcacgc tcgtctactt
     2401 ccgctgcgac gactgcgcta ccgagcagcg gcgcgcgacg caggcgggcg gcaagatcca
     2461 caaggagaag ttcagcatcg gcgagtacgg cttcatctcg ctcgtcgtgg atcccgaggg
     2521 caacatgctc ggcctgcatt ccatgaagta gcgcccatgt cggagttcgc ggagtgggaa
     2581 agcttctacg tcatcatcgg cggcggtgcc gccgcgctga tcggcctgca attcgtcgtt
     2641 ctcacgctcc tggcggagaa gccgcagctc gcggcgccgg gtgcaggcgc tgcattcgcc
     2701 acgccgacca tcgtgcattt cag
//
LOCUS       DATDMP010000283         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1267298, whole genome shotgun sequence.
ACCESSION   DATDMP010000283 DATDMP010000000
VERSION     DATDMP010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267298"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VI195_04830"
     CDS             <1..907
                     /locus_tag="VI195_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823740.1"
                     /translation="FCSATNFLHEAQGSVARIARLDAAECVQMLERIVEAQPTVRTVI
                     FQDDIFVFTKDRRILPLCEAIIAAKKKGTLPQELQFISTNRIDAMTPERLTAMRRAGF
                     RVLGFGIESFSRQILGEFNKAQIYSNIEPTLASALELGITPFLDIILSSPRASLEDVA
                     TTLRRAWHWLQQGCEVGIYPYIIPFSGAAMAADPELRPSTVYERRRIRGTRVAWDQPA
                     KILPLDPQVCATVLAIERDYETRLTQLQRCVAHLPSRTRSLLWILSSLEPMAAHGLPI
                     ADAQQVSDAVMAKLPQQPSPIVASA"
     gene            904..2325
                     /locus_tag="VI195_04835"
     CDS             904..2325
                     /locus_tag="VI195_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6823741.1"
                     /translation="MRATGSWTLIAPAAVCALLTIWIVLAAANVQAASVSLLIYGLAN
                     LIVYTDALDLLLRLHVRRRHTATASATADTRNVSIDLVGSLPGGIRRMVPLRPYAIIA
                     SVYNLGDRLDEFTESFAAYRDRVWLISDGSTDSTVMRLLQEGWRCFDDGVNRRKPAAL
                     RLLLERLPPHIETVMVIDPDIHIRGIAGGSSAELERVIGDFQQSGAAAVCPRVMIEPD
                     GFLARFQAFEYALAFRVGRESLADYSITSGVSIYRRDALWRALREHSLSVYAEDLENA
                     VILLSQGERIYYDGRLVVSTEGPGTLGRWFSQRVGWYHGLLKVYTERLSAIWRISKRT
                     PFAGYHFIIYIGGLSLVLHLVKIFSAMLLLLSLIGGFDSLFLAHLLPRNALTSPSYFI
                     GAVGSYLALGVIALFTVVPKSERAYVAPIVPLYLLYALTHLVPMTVGFGNFIAVRLWG
                     RRLYRDHYERDAASSDAWARRAG"
     gene            2322..3137
                     /locus_tag="VI195_04840"
     CDS             2322..3137
                     /locus_tag="VI195_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6823742.1"
                     /translation="MIEPQTLPVSADLHYWEQRARRFAAEGAGLAAVCSFGMPRFYNR
                     AIDLCQRLALQRWLPSGPGIRVLDVGCGVGRWSRLLAARGASVTGIDLSPTMIAEAQR
                     RASAQPLRGQCRFLVQDLAALDAGDKFDLVLSVTVLQHIVDPRALRAALQRMVEHLAE
                     DGTLILLEAAPERALGQCDSAIFRARPRSSYRRLFAECGLEVRATSGVDPAPFRTWLL
                     PHLPRCAPAVRRVALAAVTALSLPIDLPFGRALVARSWHAVFVLRHARNSHAH"
     gene            3127..>3565
                     /locus_tag="VI195_04845"
     CDS             3127..>3565
                     /locus_tag="VI195_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823743.1"
                     /translation="MRTSVRIAVAVGVLLLLGGLATLGWWYQTRQQHPAGQTGGTLIV
                     EVTSDADRGPGTLREALFIVAAANGNADVRLKVKSVAPETPLPPLVNPHGVRIIAQQG
                     GTQIDGHALSGGPVLDIAGANTSIEGLQLRNCPGSAILLRATQF"
BASE COUNT          524 a         1242 c         1176 g          623 t
ORIGIN      
        1 cttctgctcg gcgaccaatt tcctgcacga agcgcagggc agcgtcgcgc gcattgcgcg
       61 cctcgatgcg gcggaatgcg tacagatgct cgagcgcatc gtcgaggcac agcccacggt
      121 gcgcaccgtc atctttcagg acgacatttt cgtcttcacc aaggatcgcc gcatcctgcc
      181 gctgtgcgag gcgatcatcg ccgcaaagaa aaagggtacg ttgccgcagg agctgcagtt
      241 catcagcacc aaccggatcg atgcgatgac gccggagcga ctcacggcca tgcgccgggc
      301 cggattccgg gtgctcggct tcggcatcga aagtttctcg cggcagatat tgggtgagtt
      361 caacaaggcg cagatctact ccaacatcga gccgacactg gcgagcgcgc tcgagctcgg
      421 tatcacccct tttctggaca tcatcctcag ctccccaagg gcgagtctcg aggatgtcgc
      481 caccacgctg cgccgcgcgt ggcactggct gcagcagggc tgcgaggtgg gcatctaccc
      541 gtacatcatc ccgttctcgg gcgcggccat ggcagcagat cccgagctga gaccttccac
      601 ggtgtacgag cggcggcgca tccgcggcac gcgggtcgcg tgggaccagc cggctaagat
      661 cctgccgctc gatccccagg tgtgcgccac ggtgctcgcc atcgagcgcg actacgagac
      721 gcgcctgacg cagctgcagc ggtgcgtcgc gcatctaccc tcgcgaaccc gttcgctgtt
      781 gtggatcctg tcctcgctcg agcccatggc ggcgcacggc ctgccgatcg ccgacgcgca
      841 gcaggtgagc gacgcggtca tggcgaaact cccgcagcag ccttcaccga tcgtagccag
      901 cgcatgagag cgaccgggtc ctggacattg atagcgcctg cggcggtgtg cgcgttgctg
      961 acgatctgga tcgtgctcgc ggcggcgaac gtgcaggcgg cgagcgtctc gctgctcatc
     1021 tatgggctcg ccaacctgat cgtgtacacc gacgcgctcg acctgctgct gcgactgcac
     1081 gtccgccgcc gccacaccgc taccgcgagt gccaccgctg acacccgcaa cgtgtccatc
     1141 gacctggtcg ggtcgctccc cggaggcatc cgccgcatgg tgccgctgcg gccttacgcc
     1201 atcatcgcct cggtgtacaa cctcggcgat cggctggatg agttcaccga gtcctttgcc
     1261 gcctaccgcg atcgggtctg gctcatcagc gacggctcga ccgacagcac cgtgatgcgg
     1321 ctgctgcagg aggggtggcg ctgcttcgac gacggcgtca atcgccgcaa gcccgccgcg
     1381 ctgcggctgc tgctcgagcg gttgccgccg cacatcgaga ccgtcatggt catcgacccc
     1441 gacatccaca tccgcggcat cgccggcggc agcagcgcgg agctcgagcg ggtcatcggc
     1501 gacttccagc aatctggagc ggctgccgtc tgcccacggg tgatgatcga gccggatggg
     1561 ttcctggcac gctttcaggc tttcgagtac gcactcgcct tccgcgtcgg ccgcgagagc
     1621 ctcgccgatt acagcatcac ctcgggggtc tccatttacc gccgcgatgc gctgtggcgt
     1681 gcactgcgcg agcactcgct ctcggtctac gcggaggatc tggagaatgc agtgatcctc
     1741 ctcagccagg gcgagcgcat ctactatgac ggccggctgg tcgtgagcac cgagggaccc
     1801 ggtacgctcg gccgctggtt ctcgcagcgc gtcggctggt accacggcct gctgaaggtc
     1861 tacaccgagc ggctgagcgc gatctggcgc atcagcaagc gcacgccctt cgcgggttac
     1921 cacttcatca tctatatcgg cggcctgtcg ctggtgttgc atctggtcaa gatcttcagc
     1981 gcaatgctgc tgctgctgag cctgatcggc gggttcgaca gcctgttcct cgcgcatctg
     2041 ctgccgcgca atgcgctcac cagcccttcc tacttcatcg gggcggtggg cagctatctc
     2101 gccctcgggg tgatcgcgct gttcaccgtc gtgccgaagt ccgagcgcgc ctatgtcgcg
     2161 ccgatcgtgc cgctgtacct gctgtacgcg ctcactcacc tggtgccgat gacggtcggc
     2221 ttcggcaact tcatcgccgt gcggctgtgg gggcggaggc tgtaccggga tcactacgag
     2281 cgcgacgctg cctcctcgga tgcctgggcg cggagggccg gatgatcgag ccgcagactc
     2341 tgccggtctc tgccgacctg cactactggg agcagcgcgc gcgccgcttc gcggccgagg
     2401 gcgcggggct cgcggccgtg tgctccttcg gtatgccgcg cttctacaac cgcgccattg
     2461 atctgtgtca gcgcctggcg ctgcagcgct ggctacccag cgggccggga atacgcgtac
     2521 tcgatgtcgg ttgcggcgtg ggccgctgga gccgactgct cgccgcgcgc ggcgcgtcgg
     2581 tcaccggcat cgacctcagt cccaccatga tcgccgaagc ccagcgccgt gcgagcgcgc
     2641 agccgctgcg cgggcaatgc cgttttctgg tgcaggacct ggcagcgctc gacgccggcg
     2701 acaagttcga tctcgtgttg agcgttaccg tgctgcagca catcgtcgat ccgcgcgcgc
     2761 tgcgcgccgc gctgcagcgc atggtggagc atctggccga ggacggcacg ctcatcctgc
     2821 tcgaggcggc gccggagcgc gcgctcggtc aatgcgacag cgcgattttc cgtgcccggc
     2881 cgcgcagcag ctatcggcgg ctgttcgccg agtgcggtct cgaggtgcgc gccaccagcg
     2941 gcgtcgaccc ggcgccgttc cgcacctggc tgctgcctca cctgccgcgc tgcgcgccgg
     3001 ccgtgcgccg cgtggcgctg gcggcggtca ccgcgctgtc gctgcccatc gatcttccct
     3061 tcggccgcgc gctggtcgcg cgctcgtggc acgcggtctt cgttctcagg cacgcacgga
     3121 actctcatgc gcactagcgt acgtatcgca gtggcggtcg gtgtgctgct gctgctcggt
     3181 ggtctggcga cgctaggctg gtggtaccag acacggcagc aacacccggc cggtcagacc
     3241 ggcggcacgc tcatcgtgga agtgacgagc gacgccgacc gcgggccggg cacgttgcgc
     3301 gaggcgctgt tcatcgtggc cgcagccaac ggcaacgcgg acgtcaggct caaagtgaag
     3361 agcgtcgcac ccgagacgcc gctgccgccg ctcgtcaatc cgcacggcgt gcgcatcatc
     3421 gcgcagcagg gcggcacgca gatcgacggc cacgcgctca gcggcggccc ggtgctcgac
     3481 attgccgggg ccaacacctc gatcgaaggc ctgcagctgc gcaattgccc gggcagcgcc
     3541 attctgctgc gcgccacgca atttc
//
LOCUS       DATDMP010000284         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_600056, whole genome shotgun sequence.
ACCESSION   DATDMP010000284 DATDMP010000000
VERSION     DATDMP010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600056"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VI195_04850"
     CDS             complement(<1..646)
                     /locus_tag="VI195_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6823744.1"
                     /translation="MASNTELHARRQQAVPRGVTNSLAVYAERAANAELWDVEGRRYI
                     DFASGISVLNTGHVHPKVRAAIARQLEKLTHTCFQVTPYESYIALAEALNILAPGSGP
                     KKTLFLTTGAEAVENALKIARFHTRRSAIIAFSGGFHGRTLACISLTGKVQPYKTGFG
                     PMLPEIYHLPFPMPYHGVTVEHALAALEQLFKADVDPARVAAIIIEPVLGEGGFY"
     gene            complement(682..1143)
                     /locus_tag="VI195_04855"
     CDS             complement(682..1143)
                     /locus_tag="VI195_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6823745.1"
                     /translation="MITLNLNGKNTTLDVEGEMPLLWVLRDILGLTGTKYGCGMALCG
                     ACTVHLEGAPVRSCVTPLNAASGKRVTTIEGLSPDNSHPVQRAWIELDVPQCGYCQSG
                     QIMSAAALLAQTPQPSDTDIDAAMAGNICRCGTYQRIRAAIHRAAALKAGG"
     gene            1295..2353
                     /locus_tag="VI195_04860"
     CDS             1295..2353
                     /locus_tag="VI195_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6823746.1"
                     /translation="MSQTAATSSTSAPISTEVVIIGAGPCGLFQVFELGLLGIGAHVV
                     DSLRHPGGQCAELYPDKPIYDIPGLPVCGAQELVDRLLQQIKPFKAPLHLGHEVAQFA
                     RRADGRFHLTTAGGVSFDAGAVVIAAGLGSFQPRRIGVEGAEVFEGGAIQYRVRNAAE
                     LAGKHLVIFGGGDSALDWTLELLPGSASLTLVHRRSEFRAAPASVARMRELVAAGRMR
                     CFEALPHSLIVADAALRGINIRTPDGTLTALAAEQLLVFFGLHPKLGPIAEWGLALEK
                     KALKVDPEKFQTSVAGIFAVGDINTYPGKKKLILSGFHEAALAAFAIQHHLYPQKKQF
                     LQYTTTSPLMHQRLGVPD"
     gene            2384..2848
                     /gene="azu"
                     /locus_tag="VI195_04865"
     CDS             2384..2848
                     /gene="azu"
                     /locus_tag="VI195_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEY6823747.1"
                     /translation="MTVMRHSAVALVLLAALALPRFARAADPCQLEITANDQMQYDKQ
                     TLSVPASCTQVTLTLHHAGKLPREAMGHNWVLVNGADFNAVANAGTGAGLADDYVQPG
                     DKRVLAHTKVVGGGQTTSVSFSTSGLKPGGDYRYLCTFPGHYALMRGTFKFG"
     gene            2860..>3383
                     /locus_tag="VI195_04870"
     CDS             2860..>3383
                     /locus_tag="VI195_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEY6823748.1"
                     /translation="MDQRLRDLLQLLELERLELNLFRGASRDIGSPQVFGGQVLGQAL
                     SAAAATVEARVVHSLHAYFLRRGDYNAPIVYEVDRSLDGHSFSNRRVVAIQHGQQIFN
                     MTASFQAAEMGFDHQIVIPEVPPPESLAPSDGPPADVLAQLPERVRRFFQQPRPFEFR
                     MVRPIEYLQPRHEAP"
BASE COUNT          543 a         1137 c         1118 g          585 t
ORIGIN      
        1 ggtagaaacc tccttcaccc agcaccggct cgatgatgat cgccgcgact cttgccggat
       61 ccacgtccgc cttgaacagc tgctccagcg ccgcgagcgc gtgctcgacc gtcacgccgt
      121 gatacggcat ggggaagggc aggtgataga tctccggcag catcggcccg aagccggtct
      181 tgtagggctg caccttgccc gtgagactga tgcaggcgag cgtgcggcca tggaaaccgc
      241 cggaaaaggc gatgatggca gagcggcgcg tgtggaagcg cgcaatcttg agcgcgtttt
      301 ccaccgcctc cgcaccggtg gtcaggaaga gggtcttctt cgggccgctg cccggcgcga
      361 gtatgttgag cgcctcggca agcgcgatat aactctcgta cggcgtcacc tggaaacagg
      421 tatgtgtcag cttctccaac tggcgcgcga tggccgcgcg caccttggga tgcacatgac
      481 cggtgttgag taccgagatg ccgctggcga agtcgatgta gcggcggccc tcgacgtccc
      541 acagctcggc gttggcggcg cgctcggcgt ataccgcgag cgagttggtg accccacgtg
      601 gcacggcctg ctgacgccgc gcgtgtaatt cagtgttgga tgccaagata ctatcctccg
      661 agtgagggcg cgcggcgcgc gtcagccgcc ggctttgagg gccgcggcgc ggtgaatggc
      721 agcgcggatg cgctggtagg tgccgcagcg gcagatattg ccagccatgg cggcgtcgat
      781 atcggtatcg ctcggctgcg gggtctgtgc gagcagtgcg gctgccgaca tgatctgtcc
      841 ggactggcag tagccgcact gcggcacatc gagctcgatc caggcgcgct gcaccgggtg
      901 actgttgtcc ggcgagaggc cctcgatggt ggtgacacgc ttgccgctcg cggcgttcag
      961 cggcgtcacg cacgagcgca ccggcgcacc ttccaggtgc acggtacagg caccgcacag
     1021 tgccatgcca cagccgtatt tcgtgccggt gagcccgagg atgtcgcgca gcacccacag
     1081 cagcggcatc tcgccctcga catcgagcgt ggtgttcttg ccgttgaggt tgagtgtgat
     1141 catccgctcc ccagctcgcg gtctgcggcc gatgcgtact atacggcgcg gcgcgcgcgc
     1201 cgtgcagctg catggcctgc ggcagcgccc gagcgctgcc ggcgcggacc ctctacaatg
     1261 cggtcccgct cgccaagtcc gtgaaagtca tgccttgagc caaactgccg ccacttcctc
     1321 cacttcggcg ccgatcagca ccgaagttgt cattatcggc gctggtccgt gcggcctctt
     1381 ccaggtattc gagctgggcc tgctcggcat cggcgcccat gtggtcgact cgctgcgtca
     1441 cccgggcggg cagtgcgccg agctctaccc cgataagccc atctatgaca ttcccgggct
     1501 gccggtctgc ggcgcccagg agctggtcga ccggctgctg cagcagatca agcccttcaa
     1561 ggcgccgctg catctcggtc acgaggtggc gcagttcgcg cgccgcgcgg acggacgttt
     1621 tcatctgacg accgcgggcg gtgtcagctt cgatgcgggc gcggtggtga tcgccgcggg
     1681 actcggctcc tttcaaccgc ggcgcatcgg cgttgaaggc gccgaggtct tcgagggcgg
     1741 tgccatccag taccgcgtgc gcaatgccgc cgagctcgcc ggcaaacacc tggtgatttt
     1801 cgggggcggt gactcggcgc tcgactggac gctcgagctg ctgccgggct ctgccagcct
     1861 gacgctggtc catcgccgca gcgagtttcg tgccgcgccg gcctcggtcg cgcgcatgcg
     1921 cgagctggtc gcggccggcc gcatgcgttg cttcgaggcg ctgccgcact cgctcatcgt
     1981 cgccgacgca gcgctgcgcg gcatcaacat ccgcaccccc gatggcacgc tgaccgcgct
     2041 agccgccgag cagctgctgg tgttcttcgg cctgcacccg aaactcggac cgatcgcaga
     2101 gtggggactg gcgctggaga agaaagcgct caaggtcgac ccggagaaat tccagaccag
     2161 cgttgccggt attttcgccg tcggcgacat caacacctac ccgggcaaga agaagctgat
     2221 cctgtcggga tttcacgaag ccgcgctggc ggccttcgcc atccagcatc acctctatcc
     2281 gcagaagaag cagttcctgc aatacaccac gaccagtccg ctcatgcacc aacgcctggg
     2341 agtaccggat tgatgggcca gatctcgagg aggaaaggcc gccatgaccg tgatgcgcca
     2401 tagtgccgtt gccctggtgc tgctcgccgc gctcgcgctg ccgcgtttcg cgcgcgccgc
     2461 cgatccgtgc cagctcgaga tcaccgccaa cgaccagatg cagtacgaca agcagacgct
     2521 cagcgttccg gcgagctgca cgcaggtgac cctcacgctg caccacgccg gcaagctgcc
     2581 gcgcgaagcg atggggcaca actgggtgct ggtgaacggg gccgacttca acgcggtcgc
     2641 caatgccggg acgggggcgg gccttgccga cgattatgtt cagcccggcg acaagcgcgt
     2701 actcgcccac accaaggtag tcggcggcgg tcagaccacc agcgtcagct tctcgacctc
     2761 gggcctgaaa ccgggcggcg attatcgcta tctgtgcacc tttcccggtc actacgcatt
     2821 gatgcgcggc accttcaagt tcggctgaag cccccgcgga tggatcagcg ccttcgggat
     2881 ctgctgcagc tgctcgagct cgagcggctc gagctcaacc tgttccgcgg cgcgagccgc
     2941 gacatcggca gcccgcaggt gttcggcgga caggtgctcg gccaggcgct gtcggcggcg
     3001 gccgcaacgg tggaggcgcg agtggtgcac tcgctgcacg cctatttcct gcggcgcggc
     3061 gactacaacg ccccgatcgt ctacgaggtc gaccgcagcc tcgatggcca cagcttttcc
     3121 aaccgccggg tggtggccat tcagcacggt cagcagattt tcaacatgac cgcctctttc
     3181 caggcggccg agatgggttt cgaccatcag atcgtcatac ccgaggtgcc accgcccgag
     3241 agtctggcgc cttccgacgg cccgccagcc gatgtgctgg cgcagctgcc cgagcgcgtg
     3301 cgacgcttct tccagcagcc gcgaccgttc gagttccgca tggtgcggcc gatcgaatat
     3361 ctgcagccgc gccacgaggc gcc
//
LOCUS       DATDMP010000285         4272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_933616, whole genome shotgun sequence.
ACCESSION   DATDMP010000285 DATDMP010000000
VERSION     DATDMP010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933616"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..878)
                     /locus_tag="VI195_04875"
     CDS             complement(117..878)
                     /locus_tag="VI195_04875"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEY6823749.1"
                     /translation="MSPTANALIVLASVFGSGLLGLSLRGVIPAQHLQEDSLGMVRLC
                     SGVLATLAALVLGLLVASAKANYDRVNDQVTQAATAIVLLDRTLAQFGPQTKEARGLL
                     RAAVAGAVDTVFSKHSRGVADLDDPQRLAIGERLEAQIRTLSAENEVQRTLQGNALAL
                     ASEVAKMRLLTITQAEGSIPGMFLIVLVLWLAIMFAGFGLVTARNPTVIIALFLCALS
                     LAGAVFMIEELNRPLEGLMKISSTPLRYALAHIGQ"
     gene            878..1606
                     /locus_tag="VI195_04880"
     CDS             878..1606
                     /locus_tag="VI195_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823750.1"
                     /translation="MTSPITENENVTITRYLAECDLSSRLATVPYEAHGANLARRSRR
                     EHMTTTIKPLAPEGTSPARREDDPPVWNRTRITFISTCPKCGHDRFQHGYTRRILFDL
                     LKAGSKIDAYCIDCNVCWPISERERRAISPRLNARLQICNGARLAWCSQRRRPREPNW
                     RDRRAPSKSQMGLRQALRTLRAIRLRYEVDRRNADARLRSVLVALSCADMLWISAAIE
                     ALQEAIAPGATESGVPPATAPQET"
     gene            complement(1797..2825)
                     /locus_tag="VI195_04885"
     CDS             complement(1797..2825)
                     /locus_tag="VI195_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6823751.1"
                     /translation="MTHMPAEDERVTRHLGCGESSMVKSLRIRRRSPALVLSVLLLAG
                     GESASPADPPGPAATEGTPPSSSHGGFLSSLKQAFSQNVERDVVWAHFDVGSAPDTHR
                     FYCLLDPKTGKREPNGVAGEPFQRPDGTTGLKGPAISPVSCSDAEQKGVLVTSGYAVK
                     TGAGGASGSMAAPAPTPPAAPAAPAAASASATAAAPPAASAAVAAAAPAAASHAQGSD
                     LRAQMEAANASFLAAYNTQNGAAFKGMYTPDAMLFAPSTQPLVGAEAIGAFWADRIKG
                     GNRKNHTFEIVSVWADGRFAYQVARYTVDVVNDKGEIFKSVGNTVRIFERQADGKWLT
                     KVHIFNSY"
     gene            complement(3554..>4272)
                     /locus_tag="VI195_04890"
     CDS             complement(3554..>4272)
                     /locus_tag="VI195_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823752.1"
                     /translation="SSAPPPLPHDSLSDTQVLRMLEVRCSPEEPEGDERNGERVMLLR
                     PEDTSTRQALKEAVAQGAPVSFAVQLHWSAQPLELSRVPALPVFKGYTLYATESRREG
                     RSRFFLRLGFFGDPVSATQIAAQVRSTFASAAVIPVAEPEVMRAREACTHGSSIPCLN
                     VQSVEPALDASGVSTTPESKSARDVPRRVSRVDSLEMTLRRLAAREKWTDPDSLSESG
                     VRHLKVEVQKFRGARPRASK"
BASE COUNT          807 a         1385 c         1355 g          725 t
ORIGIN      
        1 agaacacgtg aactgtgcga ccaagccagg cagcagcagg cgcgtgaaga tgcgagatac
       61 ggaatggcag accaattcgt ctacaagtgc gtggaacgca agagttagcc gcagtgtcac
      121 tggccgatgt gggccagtgc gtagcgaagc ggcgtgctcg agatcttcat caagccctcg
      181 agcggccgat tcagctcctc gatcatgaag accgcgcctg cgagtgacag cgcacagaga
      241 aacagcgcga tgatgacggt cggattcctc gccgtaacca ggccaaatcc ggcaaacatg
      301 atggccagcc agagcaccag tacgatcagg aacattcctg gaatcgagcc ctccgcctgg
      361 gtgatggtca gcagtcgcat tttggcgacc tcgctggcga gcgccagagc attcccctgc
      421 agcgtgcgct gcacctcgtt ctcagcggac agcgtgcgta tttgcgcctc gagcctctct
      481 ccgatggcca acctctgagg atcatcgagg tctgccacac cgcgactgtg cttcgagaac
      541 accgtgtcga cggctccggc cacggccgcg cgcagcaggc cgcgggcttc cttggtttgc
      601 ggaccgaact gggccagggt gcggtcgagg agcacaatgg cggtcgcggc ttgggtgacc
      661 tggtcgttta cgcgatcgta gttcgccttg gccgaggcaa ccagcagtcc aagcaccagc
      721 gcggccaacg tggcgagcac gcccgagcac agccgcacca taccgagcga gtcctcttgc
      781 agatgctgcg cgggtatgac gccgcgcagc gacaagccga gcagcccgct tccgaatacg
      841 ctggcgagta cgatgagagc gttagccgtc gggctcattg acatccccaa taaccgaaaa
      901 cgaaaacgtc acgatcaccc gctatcttgc cgagtgcgac ctgagtagcc gtttggccac
      961 tgtcccctac gaggcccacg gcgctaacct cgcccgtcgt tccaggaggg agcatatgac
     1021 gacgacaatc aagccgctcg cacctgaagg cacgtcgccg gcacgcaggg aggacgaccc
     1081 accggtgtgg aaccgtacgc gcatcacatt catatcaacc tgtccgaagt gtggccacga
     1141 ccgcttccag cacggatata cgcgccgcat cctcttcgac ctgctgaagg ccggcagtaa
     1201 gatcgatgcc tactgcatcg attgcaatgt atgttggccg atcagcgagc gcgagcgccg
     1261 cgcgatctca ccacgattga acgccagact ccagatctgc aatggcgctc gcttggcctg
     1321 gtgtagtcag aggcgcaggc cccgcgagcc gaattggcgc gaccgaagag ctccctccaa
     1381 gagccagatg ggcttacggc aggcgctgcg caccttgcgc gccattcgcc tgcgatacga
     1441 ggtcgatcgc aggaacgcag atgcgcgcct gcggagtgtg ctggtcgccc tgagctgtgc
     1501 cgacatgctg tggatcagcg cagcgatcga ggcgttgcaa gaagccatcg ctcctggcgc
     1561 aaccgaatcc ggcgtgccgc cggccacggc cccgcaggaa acgtgaactg gccgtacgac
     1621 ttaatcagtt gagatgtgct gcaatccatc cgccgtcgcg cattaggcgc ccccgcgcgg
     1681 cgacacaatg gcctcgccgg gtgtccgacc acacatcccc ggcggggcct ctctctatac
     1741 ggcacgcata aaaatccgcc tccaggcggc gctcgaagct caccggcctg agcgcgctaa
     1801 tacgaattga agatgtggac cttggtgagc catttgccgt ccgcctgtct ttcaaaaata
     1861 cgcaccgtgt ttccaaccga tttgaagatt tcccccttgt cgttgacgac atcgaccgtg
     1921 tagcgggcaa cctgatacgc gaacctgccg tctgcccaca cactgacgat ctcgaacgta
     1981 tggttctttc tgttgccgcc cttgatgcgg tcagcccaaa acgctccgat agcctctgct
     2041 ccaacgagcg gctgcgtgct cggagcgaac agcatggcgt ctggggtgta catgcctttg
     2101 aacgccgccc cgttctgggt gttgtacgcg gcgagaaagc tcgcgttggc cgcctccatc
     2161 tgcgcgcgca ggtcagagcc ctgggcgtgc gacgctgccg ccggagcggc cgcagccacg
     2221 gcggcggatg ccgcaggcgg cgcagctgca gtggctgacg ccgatgcggc agcgggcgca
     2281 gcgggagcgg cgggcggcgt gggtgctggg gcagccatcg agccggatgc gccgcctgcg
     2341 ccggtcttga ccgcgtagcc gctggtgacc agaactccct tctgttccgc gtcgctgcaa
     2401 ctcaccggag agatcgccgg acccttgagc cccgtcgtcc cgtcagggcg ctggaacggc
     2461 tccccggcga cgccatttgg ctcgcgtttt cccgtcttgg gatcgagcag acagtagaag
     2521 cgatgcgtgt cgggagctga gcccacatcg aagtgtgccc acaccacatc gcgctcgacg
     2581 ttctggctga aggcctgctt gagggacgaa agaaatccgc catggctact cgagggcggg
     2641 gtgccctcgg tggctgcggg gccgggtgga tccgccgggg aagcgctctc cccgccggca
     2701 agcaagagaa cgctcagtac cagagccggc gaacggcgcc ggattcgcag cgacttcacc
     2761 atggaggact ctccacaccc aagatgtcgg gtgacgcgtt cgtcttctgc gggcatgtgc
     2821 gtcacccgca gcaagcatgc acccggcctg aggaaggcgg caaatccaga cagcgaaacg
     2881 ccgggaagaa tgagcagcgc ggcggcggcg cgtgcttgga tgtcagggag acgccaccgg
     2941 caggctattc cctgtcggcc ggtgggcccc catcaccgca atagctggtc gacacaagcg
     3001 agcgcggcga cgacgcgatt caatcgctga ccaacatatt gcgtcaagct gtcgctcatt
     3061 ggtgactgac tccgcacggt gcgtgcgcgg ccgcctggcg gcgctcgcgc cgcgacatcc
     3121 cgcagcacgc ggcgcaggat gtgtacggca gggtgttagc gaccgaatcg catcctgggt
     3181 cggctcgctc gcggtttacc gatcaatcca tcgggctcct ggacatcatc ggcagaattg
     3241 gcgcgagcgt agttctgcat ccgcagcagg gcgctatcca gattgcacgt ggcccgtagc
     3301 agtcccgcaa gccacagacg gtaggggagc tctggctctg caccatgctc tgagccagag
     3361 gatgcacagg actcggctcc gatcccaagg acgaggtacc tgccctgccc gtcagtcggc
     3421 aacgcgcgca acgcgagcaa accgatcagg agcaatgcca cctgcaaacg catggcggca
     3481 tggtgtgctc cttgggtgat tgcgcgccgc gtgcccggtc acaataaacg atgacgccag
     3541 cacgcggccg aggtcatttg ctggcgcgtg gacgggctcc tctgaacttc tgcacctcga
     3601 ctttcagatg acgcacgccg ctctcgctga gtgagtcggg atcagtccat ttctccctcg
     3661 cggcaagtcg cctgagagtc atctccaggc tgtcgacccg ggagactcgg cgcggcacat
     3721 cccgtgcgga ttttgattcg ggcgtggtgg aaactccgct ggcatcgagc gccggttcga
     3781 cgctctgcac gttgaggcag gggatcgagg agccgtgcgt gcaagcctcg cgggcacgca
     3841 tgacctccgg ctcggcaact ggtattacgg ctgcggaagc aaaggtagag cgcacctgcg
     3901 cggcgatttg cgtcgccgaa accggatctc cgaagaaccc cagccgtagg aagaagcggg
     3961 aacgcccctc gcgccggctc tcagtggcgt agagcgtgta tcccttgaat accgggagcg
     4021 ccggcactcg gctcagctcg agcggttgcg ccgaccagtg gagctgcacg gcaaaggaca
     4081 ccggcgcacc ctgagcgacg gcctccttca aagcctgccg ggtgctggtg tcctcgggac
     4141 ggagcaacat tacccgctcg ccatttctct catcgccctc gggctcctct ggggagcagc
     4201 gcacttccaa catcctcagg acctgtgtgt cgctcaacga gtcgtgcggc aacggcggcg
     4261 gcgcgctgct ct
//
LOCUS       DATDMP010000286         3809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_822774, whole genome shotgun sequence.
ACCESSION   DATDMP010000286 DATDMP010000000
VERSION     DATDMP010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822774"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VI195_04895"
     CDS             <1..754
                     /locus_tag="VI195_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6823753.1"
                     /translation="PVPLRALPPLDLVLISHDHYDHLDYPTIRQLRRRAMPFVTSLGV
                     GAHLEAWGVPPRQIIELDWWESYTLPNAELTVTATPSQHFSGRTPSSRNSTLWSSLVI
                     RSPRHTVFFSGDTGLTTEYQSIRERFGRFDLTMLEIGAFHPAWGDMHLGPENALQALA
                     LLGGGRFLPIHWGTFALGMHPWDQPAEALLQLAPRSGAQLLMPRLGQPIEPAHTELIE
                     PWWREADARGRRRGLGSLAGGALPKTLPWPLD"
     gene            complement(772..1797)
                     /locus_tag="VI195_04900"
     CDS             complement(772..1797)
                     /locus_tag="VI195_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY6823754.1"
                     /translation="MRMLPVVGMLFTVTVLAATTPAPQTHRLEATPATVAYGYYWAQA
                     APVLRIASGDIIDVDTLLTNTPAGLEKAGVSQARIQSSLKSIVTEVTGERRGPGGHIL
                     TGPVYVAGAEPGDVLEVKILGIDLAIDYGYNGCSGVLPENCEAGVPVRIIALDRSTMR
                     AEFAPGIIVPLRPFFGSMGVAPPPEAGRVSSNPPGRHAGNLDNRELIVGSTLYIPVFA
                     TGALFEVGDGHVAQGDGEVDQTAIETSLRGRLQLTVRKDMKLTWPRAETPTDYISMAT
                     DPDLLIATKTAIQDMVDFLMAARHLTRHEAYQLVSVAGNVAVTQLVDKPNVGVHVRMP
                     KSIFVEH"
     gene            1905..3116
                     /locus_tag="VI195_04905"
     CDS             1905..3116
                     /locus_tag="VI195_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6823755.1"
                     /translation="MPSAITTTEVFLIAMGIIFTVPYLIWRVLRTDYYAPLVVVQIIA
                     GILLGPGILGRFFPAYYAYVFRPAVIAPLNGIAWWAAMLFVCIAGIELDLKKAWRHRA
                     ESGIAAALALGVPLLFGGAAAIGMLRYPGWGGARAADWQFVLGVGMACAVTALPVLIL
                     LMEKLEILRQPLGQRLLRYASLDDIAIWSVLAVILMDWSRVGRQLGFLLVLALASGLF
                     RQLMPALAERDRWYAALIWVLLCGWAADWSGLHFMVGAFLSGAVMDAEWFEQAEMDRF
                     RQYVLLGFMPVFFLSTGLRTTWELGGSAVLLAAAVLLLAALSGKLIGTVIAGRFLGWA
                     RREAVIIGWLLQTKGLVMILFINVLLDKSLITSGTFTALLLMGVVSTMLTVPMVAGRL
                     RRERELVYKAA"
     gene            3499..>3809
                     /locus_tag="VI195_04910"
     CDS             3499..>3809
                     /locus_tag="VI195_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823756.1"
                     /translation="MYAIDVRTPLTYKRSRAAASPGRRRSPGSERYAMKSEIICFDQM
                     LANVRLATTRVAGSRRTSATHEVNALTVKRLRGWTRLSQPEFARLLGVELSTLRNWEQ
                     GR"
BASE COUNT          568 a         1252 c         1288 g          701 t
ORIGIN      
        1 gccggtgcca ctgcgcgcgc tgccgccgct cgatctcgtt ctcatctcgc acgatcacta
       61 cgatcatctc gattacccga ccatccgcca gctgcgccgg cgcgcgatgc ccttcgtcac
      121 ctctctcggt gtcggtgcgc atctggaagc gtggggcgta ccgccacggc agatcatcga
      181 gctcgactgg tgggagtcct acacgctgcc gaatgcggag ctgacggtga cggcgacccc
      241 gtcgcagcat ttctccggcc ggaccccgag cagccgcaac agcacgctgt ggtcatcgct
      301 ggtcatccgc tcgccacgcc acacggtgtt tttcagtggc gataccgggc tcaccaccga
      361 gtaccagagc atccgcgagc gattcgggcg gttcgatcta acgatgctgg agatcggcgc
      421 gtttcatccc gcgtggggcg acatgcacct cgggcccgag aacgcgttgc aggcgctcgc
      481 gcttcttggc ggtggccgct ttctccccat ccactggggt acgttcgcac tcggcatgca
      541 cccctgggat cagccggccg aagcattgtt gcagctcgca ccgcgcagcg gcgcgcagct
      601 cctgatgccg cgtctcggac aaccgatcga gcccgcacac acggagctca tcgagccctg
      661 gtggcgcgag gccgacgcgc gcggccggcg gcgggggctc ggctcgctcg cgggcggagc
      721 acttccgaag acgctcccct ggccgctcga ctgacgcgag aggcgatccg ctcagtgctc
      781 gacgaagatg ctcttcggca tccgtacgtg cacgccgacg ttcggcttgt cgaccagctg
      841 cgtgaccgcg acgttgcccg cgacgctgac gagttgatag gcctcatggc gcgtgagatg
      901 tctcgcggcc atgaggaaat ccaccatgtc ctgaatggcg gtcttggtgg cgatgagcag
      961 atccgggtcg gtcgccatgc tgatgtaatc ggtgggtgtc tccgcgcgcg gccaggtgag
     1021 cttcatgtcc ttgcgcaccg tcagctgcag gcggccgcgc agcgaggtct cgatcgccgt
     1081 ctgatcgacc tcgccatccc cctgcgccac gtgtccgtcg cccacctcga acagcgcccc
     1141 ggtggcgaac accggtatgt agagcgtgct gccgacgatg agctcgcggt tgtcgagatt
     1201 gccggcatga cgtcccggtg ggttgctgct cacgcgcccg gcctcgggcg gcggtgcgac
     1261 gcccatgctg ccgaagaacg gccgcagcgg cacgatgatg cccggtgcaa actcggcacg
     1321 catcgtgctg cggtcgagcg caatgatccg caccggcact cccgcctcgc agttctccgg
     1381 caacacgccg ctgcagccgt tgtagccata gtcgatcgcc aggtcgatgc cgaggatctt
     1441 cacctcgagc acatcgccgg gctccgcccc cgcgacgtac accgggccgg tgagaatgtg
     1501 accgccgggc ccgcgacgct cgccggtgac ttcggtgacg atcgacttga gtgagctctg
     1561 gatgcgcgcc tgcgacacgc ccgccttctc gagcccggct ggcgtattcg tcaagagcgt
     1621 gtcgacatcg atgatgtcgc ccgaggcgat gcgcagcacc ggcgccgcct gcgcccagta
     1681 gtagccgtag gcaacggtgg caggcgttgc ctcgagacga tgggtctgcg gtgcgggtgt
     1741 ggtggcggcg agcacagtga ccgtgaagag catgccgaca acggggagca tacgcatgat
     1801 gactcctcat ccgagtgcgc caatatatcg gcatgacgcc gcacacgggg actcacgact
     1861 ccacggctgg acgcggcgag ccgctttcgg tatgagtgtt ggaaatgccc agcgcgatca
     1921 cgaccaccga agtcttcctg atcgcgatgg gcatcatctt cacggtgccg tatctgatct
     1981 ggcgcgtgct gcgcaccgat tactatgcgc cgctcgtcgt ggtgcagatc atcgcgggca
     2041 tactgctcgg ccccggaatc ctcggcaggt tctttcccgc ctactacgcc tacgtattcc
     2101 gcccggcggt gatcgcgccg ttgaacggta tcgcctggtg ggcggcgatg ctgttcgtgt
     2161 gcatcgcggg catcgagctc gacctgaaga aggcctggag acaccgcgcg gagtccggga
     2221 tcgccgcggc gctcgcgctc ggcgtgccgc tgctctttgg cggcgctgcg gccattggca
     2281 tgctgcgcta tccaggctgg ggcggtgccc gtgccgcgga ctggcagttc gtgctcgggg
     2341 tcggcatggc ctgcgcagtc accgcgctcc cggtcctgat cctactgatg gagaagctcg
     2401 agattctccg ccagccgctc gggcagcgcc tgctgcgcta cgccagtctc gatgacatcg
     2461 ccatctggag cgtgctcgcc gtcatcctca tggactggtc gcgggttgga cggcagctgg
     2521 ggttccttct cgtacttgca ctcgcgagcg ggctgttccg gcagctgatg ccggcgctcg
     2581 ccgagcgcga tcgctggtac gcggcactga tctgggtact gctgtgcggc tgggctgcgg
     2641 actggtcggg gctgcacttc atggtgggcg cattcctttc cggggcggta atggatgcgg
     2701 agtggtttga acaggcggag atggaccgct tccgtcagta cgtcctgctc ggattcatgc
     2761 cggtgttctt cctctcgacc gggctgcgca ctacctggga gttgggcggg agtgccgtgc
     2821 tgctcgccgc cgctgtactg ctcctggccg cgttgagcgg caagctgatc gggacggtca
     2881 tcgccgggcg cttcctcggc tgggcgcgcc gcgaagccgt cattatcggc tggctgctgc
     2941 agaccaaggg gctcgtcatg atcctcttca tcaacgtgct gctcgataag agcctgatta
     3001 ccagtggcac cttcaccgcg ctgctcctca tgggggtggt gagcaccatg ctgaccgtgc
     3061 cgatggttgc cggacggctg cgccgggagc gcgagctcgt atacaaggcg gcctgaaggc
     3121 gcggtcgggc cggaaacgag ctggaacggc aaaggctaag gtctggtctt cggcggcggc
     3181 gcggctcacc tgcgatgagg tccttcggac ggatgaggtc cttcgaacga tagttcaccg
     3241 gacggtgaac catcctaggg agtggttggt ctgtggtggg gctcgatcgg ccgggcaggg
     3301 gtcatggccc cggcgtgcca tggatcacag aatcgacgta gcgcgggcgc gacttgggtg
     3361 tcgcgcgtca cagggagcct ccggcgcccc cacctcccac cccggacgtg gcgcgcgtca
     3421 cggtagtggc tgagcggctc tggtctcctg aggcttgtgc gcccggcagg tttcggcagc
     3481 cggactaagg agtacgccat gtacgccatt gacgtacgta cgccgctgac atataaacgt
     3541 tcccgtgccg cggcctcgcc ggggcggcgg cgcagccctg gatcagaacg ctacgctatg
     3601 aagagcgaga tcatctgctt cgatcaaatg ttggcgaacg tgcgcttggc gaccacccgg
     3661 gtggccggca gccgccgtac gtcagcgacg cacgaggtta acgcgctcac tgtcaagcgg
     3721 ctgcgcggct ggacccgcct cagccagccc gagttcgcgc gcctcctcgg cgtcgagctc
     3781 agcaccctgc gcaactggga gcagggccg
//
LOCUS       DATDMP010000287         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_156156, whole genome shotgun sequence.
ACCESSION   DATDMP010000287 DATDMP010000000
VERSION     DATDMP010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156156"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="VI195_04915"
     CDS             <1..1843
                     /locus_tag="VI195_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ family protein"
                     /protein_id="HEY6823757.1"
                     /translation="EAKDRTRVVLNLDKMVPYDTRVAGNEIIVTLGATSGGQVAAAGA
                     TPAVAATAPGAATRELRSIDFRRGTDGAGRVIVKLSDPHIRINMHEVGNQIVVDFTDA
                     TVPANLARRYDASDFGTPVTGFDVARVGNGTRILITASGDFEQLAYQSDDQYVVEVAP
                     RRKANVAQEEKPVYTGERLTLNFQDIDTRAVLQLLADASGQNIVVSDSVTGSVTLRLQ
                     NVPWDQALDIVLRTKGLDKRKQDNVIIVAPQAELAAREKADLAARKDVQELAPLRSEY
                     LQVNYAKAADLAALIKTSNNSLLSPRGSVSVDERTNTLLLQDTSDRLTDIRRLVATLD
                     IPVRQVLIEARIVIVNNDFERDLGAVFGLTNVQKYGNTGLVSTAGSVAGTDQIMSSAL
                     TNVQSTGSIYPVSIPTGPQALNRYNVNLPVANPAGKIALGILGNNFLVDLELSAAQAE
                     TQANIISSPRVITANQKQATIQQGVEIPYQQSASSGATTIQFKDAVLLLKVTPQITPD
                     NRIILDLDVRDDSVGTVVVASGGVNVPSIDTREITTQVLVNDGQTVVLGGILQTTQRE
                     DTSKVPYLADIPVLGHLFKNTIHKDDKDELMIFITPKIVREGVNVTN"
     gene            1928..>2400
                     /locus_tag="VI195_04920"
     CDS             1928..>2400
                     /locus_tag="VI195_04920"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY6823758.1"
                     /translation="MREAASVFLIGPMGSGKTAVGRALARSLGLAFYDSDAEIERRTG
                     VDIPFIFEKEGEAGFRQREREAIETLTRLPRIVLATGGGAVLLPENRRRLAERGCVVY
                     LETSVAQQADRVRRGRHRPLLQRGDVAGRLRELMEVRSPLYGEIADLTVPTDGRRV"
BASE COUNT          428 a          871 c          769 g          332 t
ORIGIN      
        1 cgaggccaag gatcgcacgc gcgtggtgct gaatctcgac aagatggtgc cgtacgacac
       61 gcgcgtcgcc ggcaacgaga tcatcgtcac cctcggcgca acaagcggcg gccaggtcgc
      121 ggctgccggt gccacgcctg ccgtggcggc tacggcgccg ggcgcggcga cgcgcgagct
      181 gcgctcgatc gacttccgcc gcggcaccga cggggccgga cgggtgatcg tgaagctctc
      241 cgacccgcac atccgcatca acatgcacga ggtcggcaac cagatcgtgg tggacttcac
      301 cgacgccacc gtgccggcca acctggcgcg ccgctacgac gccagcgact tcggcacccc
      361 ggtgaccggc ttcgacgtcg cccgcgtcgg caacggcacc cgcatcctga tcaccgcgag
      421 cggcgacttc gagcagctcg cctatcagtc cgacgatcag tacgtagtgg aagtggcgcc
      481 gcgccgcaag gcgaacgtcg cccaggagga gaagccggtc tacaccggcg agcgcctcac
      541 cctgaacttc caggacatcg atacgcgtgc ggtgctgcag ctgctggccg acgccagcgg
      601 ccagaacatc gtggtgagcg actcggtcac cggcagcgtc acgctgcggc tgcagaacgt
      661 gccgtgggac caggcgctcg acatcgtgct gcgcaccaag ggcctcgaca agcgcaagca
      721 ggacaacgtc attatcgtcg cgccccaggc cgagctcgcc gcgcgcgaaa aggccgatct
      781 cgccgcccgc aaggacgtgc aggagctcgc gccgctgcgc tccgagtacc tgcaggtgaa
      841 ctacgccaag gcggccgacc tcgccgctct catcaagacc tcgaacaact cgctgctgtc
      901 cccgcgcggc agtgtctcgg tcgatgagcg caccaacacc ctgctgttgc aggacacctc
      961 cgatcggctc accgacatcc gccggctggt ggcgacgctc gacatcccgg tgcgccaggt
     1021 gctgatcgag gcgcgcatcg tcatcgtaaa caacgacttc gagcgcgatc tcggcgcggt
     1081 cttcggcctc accaacgtgc agaagtacgg taacaccggt ctcgtgagca cggccggcag
     1141 cgtcgccggc accgaccaga tcatgagctc ggcgctcacc aacgtgcaga gcaccggctc
     1201 gatctacccc gtctcgatac cgaccggccc ccaggcgctc aaccgctaca acgtcaacct
     1261 gccggtggcg aaccccgcgg gcaagatcgc cctcggcatc ctcggcaaca acttcctggt
     1321 ggacctcgag ctgtccgcgg cgcaggccga gacccaggcg aacatcatct cctccccgag
     1381 ggtgatcacc gccaaccaga agcaggcgac catccagcag ggcgtcgaga tcccctacca
     1441 gcagtcggcc tcgagcggcg cgaccaccat ccagttcaag gacgcggtgc tgctgctgaa
     1501 agtgaccccg cagatcacgc ccgacaaccg catcattctc gacctcgacg tgcgcgacga
     1561 cagcgtcggc accgtggtgg tcgcctcggg tggcgtcaac gtgccctcga tcgacacgcg
     1621 cgagatcacc acccaggtgc tggtgaacga cggtcagacg gtggtgctcg gcggcatcct
     1681 gcagaccacc cagcgcgagg acaccagcaa ggtgccgtac ctcgccgaca ttccggtgct
     1741 cgggcacctg ttcaagaaca ccatccacaa ggacgacaag gacgagctca tgatcttcat
     1801 caccccgaag atcgtgcgcg agggcgtcaa cgtcaccaac tgacggcgcg gcccgcgccc
     1861 gtcccgcgac tccgtggccg ccctgaaaag gcggccacgg cgtttttagt atcctgtcgc
     1921 gcagtccatg cgcgaagcag ccagcgtatt cctcattggt ccgatgggct cgggcaagac
     1981 cgccgtcggg cgcgcgctcg cgcgctcgct cgggcttgcc ttctatgaca gcgacgcgga
     2041 gatcgagcgg cgcaccgggg tcgacatccc gttcatattc gagaaggagg gcgaggcggg
     2101 cttccgccag cgcgagcgcg aggcgatcga gacgctcacc cggctgccgc gcatcgtgct
     2161 tgccaccggg ggcggagcgg tgctgctgcc cgagaaccgc cgccgcctcg ccgagcgcgg
     2221 ctgcgtggtg tacctcgaga cctcggtggc gcagcaggcc gatcgcgtgc gccgcggccg
     2281 ccaccgcccg ctgctgcagc gcggcgacgt cgccggacgg ctgcgcgagc tgatggaggt
     2341 gcgctcgccg ctctacggcg aaatcgccga tctcaccgtg ccgacggacg gacggcgcgt
//
LOCUS       DATDMP010000288         3813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_712261, whole genome shotgun sequence.
ACCESSION   DATDMP010000288 DATDMP010000000
VERSION     DATDMP010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712261"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VI195_04925"
     CDS             <1..200
                     /locus_tag="VI195_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823759.1"
                     /translation="FNLAYALPLAQPALSNDDLTPNILRWLRTIYARPATRACWAMGR
                     TSMVERVKILEQPHIAAGQIA"
     gene            197..952
                     /locus_tag="VI195_04930"
     CDS             197..952
                     /locus_tag="VI195_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6823760.1"
                     /translation="MSLTLYHGEPNGPSLTVLAALFAKQVPAELRYVELARGGRHALA
                     CAQEPLVAMSIEGEGPVLVADGEAMSDSVFIACYLDDVGQGAALRPADAYDRWETMKW
                     CRQMIERTAPAAAYLGCHAHPPQIDTATLKRIGSVDLRARWEEVAAGQFAAEKLEDSR
                     SKIRQAAERIEAQLAEREWLMGDYSLADLESYAWLAGMVALVPEAFAEQPRTRKWLAR
                     VKASPGVAQALSLARSSQPAASWSVGPEINRWG"
     gene            972..1634
                     /locus_tag="VI195_04935"
     CDS             972..1634
                     /locus_tag="VI195_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6823761.1"
                     /translation="MAIKLYTAATPNGWKISIALEEMALPYEVRVIDFASEDQKTDWY
                     LKLNPNGRIPTIEDEGFVVFESGAILIYLAEKTGKFLPRDVQGRSRVLQWLMFQMSGI
                     GPMMGQANVFLRYFPEKLQPAIDRYQREVTRLFRVLDRQLARHEYIAGDYSIADMALW
                     PWVSGHDYSGVSVDEFKSLQRWLTLVGSRPPVQAGRDVPIKRDRAAEIEQAQQTGPKM
                     LA"
     gene            1648..2517
                     /locus_tag="VI195_04940"
     CDS             1648..2517
                     /locus_tag="VI195_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6823762.1"
                     /translation="MIDLSKVRAIDVHVHADVSCHDPEDPVMGRFYDAASAYFKAPRE
                     RPKIPEIISLYRQQQIAFCLFTVDCESSLGAKRVSNYEIADYAAKNDDIVIPFASIDP
                     HKGSMGAREARDLIEHHGVRGFKFHPIMQDIHPADRIAYPIYEVIAAHKLPAIFHTGH
                     SGMGTGLPGGGGVRLKWGQPMLIDDVAADFPDMKIVLAHPSWPWVDESLSMALHKDNV
                     YIDLSGWSPKYFQPQIIQYANGPLKTKMMFGSDFPLIRPDKWLEAARQVGFKAEVMPG
                     IMKENAARLLGLT"
     gene            2514..3290
                     /locus_tag="VI195_04945"
     CDS             2514..3290
                     /locus_tag="VI195_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823763.1"
                     /translation="MSEFANRHVLITGASSGIGLATARLLARRGARVFLIARRDDRLA
                     AATAAIAHDGGTAAWAAADVADRAALLAAIDKAEEAFGAVAGLFANAGGGGRFAPLAE
                     YDDAQFDSLLRTNLTSVYWVMKRVLPPMLAAGSGSIVVTGSLASARGMPNNIAYVASK
                     HGVMGLARAAAAEAAPRNVRVNCVLPGLIETPMLMQLDPGAPAEAIRARLGSHVPQGR
                     IGSADELAELVCFLLSDRASHITAQTIAVDGGILGTLIPR"
     gene            3297..>3813
                     /locus_tag="VI195_04950"
     CDS             3297..>3813
                     /locus_tag="VI195_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6823764.1"
                     /translation="MTLKYYHAEPLANSLKSMVPLIEKGLPWQSIYVDLHKFEQHEPW
                     FTAINPEGQVPVLDHDGVIITHTTVINEYLEDVFPDVPLRPRDPVGAARMRYWNKFCD
                     EQVMNYVSMHGWHRMVGIIARSIESGEFDRLMERIPLPDQRTKWRTARSGFSEKDLAY
                     ATEKILYAVDKV"
BASE COUNT          680 a         1237 c         1275 g          621 t
ORIGIN      
        1 gcttcaatct cgcctacgcg ctgccgctgg cgcagccggc gctctccaat gacgacctga
       61 cacctaacat cctgcgctgg ctgcgcacca tctatgcgcg tccggcgacc cgggcctgct
      121 gggccatggg ccggaccagc atggtcgagc gcgtcaagat tctcgagcag ccacacatcg
      181 cggcggggca gatcgcatga gcctgacgct ctatcacggc gagcccaacg gcccgtccct
      241 gaccgtgctg gcggcgctct tcgccaagca ggtgccggcg gagctgaggt acgtcgagct
      301 cgcccgtggc gggcgccacg cgcttgcctg tgcgcaggag ccgctggtgg ccatgagcat
      361 cgaaggtgag ggaccggtgc tcgtggccga tggcgaagcc atgtccgact cggtgttcat
      421 cgcctgttat ctcgatgatg tggggcaggg cgcggcgctg cgccccgccg acgcttacga
      481 tcggtgggag acgatgaagt ggtgccggca gatgatcgag cgcaccgcac ccgcagccgc
      541 gtacctcggt tgccatgcgc atccgccgca gatcgacacc gcgacgctca agcggatcgg
      601 cagcgtggac ctgcgtgcgc ggtgggaaga ggttgccgcc ggacaatttg ccgcggagaa
      661 gctcgaggac agccgcagca agatccggca ggcggcggag cgcatcgagg cgcagctcgc
      721 cgagcgtgag tggctgatgg gcgactattc gctcgcggat ctcgagagct acgcatggct
      781 cgccggcatg gtggcgctcg tgccggaggc gtttgccgag cagccacgca ccaggaagtg
      841 gctcgcgcgt gtcaaagctt ccccgggagt cgcccaagcg ttgtcactgg cgcgcagctc
      901 gcagcctgcg gcgagctgga gcgtcgggcc ggaaatcaat cgctggggct gaaggcagcg
      961 ggaaggaaac catggccatc aaactctata cggcagcgac acccaacggc tggaagatct
     1021 cgatcgcgct cgaggaaatg gcgctaccgt atgaggtgcg ggtgatcgac ttcgcgagcg
     1081 aggatcagaa aaccgactgg tacctgaagc tcaaccccaa cggacgcatt ccgaccatcg
     1141 aggacgaggg cttcgtggtg ttcgagtccg gcgcgattct catttacctg gcggagaaga
     1201 ccgggaaatt cctgccgcgc gatgtgcagg gccgcagccg ggtactgcag tggctcatgt
     1261 tccagatgtc cggcatcggc ccgatgatgg ggcaggcgaa tgtgtttctg cgctacttcc
     1321 cggagaaact tcagccggcg atcgatcgct atcagcgcga ggtcacgcgg ctcttcaggg
     1381 tgctcgaccg gcagctcgcc cgtcacgaat acatcgccgg cgactattcc attgccgaca
     1441 tggcgctgtg gccatgggta tcaggacacg actactcggg tgtcagcgtc gatgagttca
     1501 agagcctgca gcgctggctc acgctggtcg gctcgcgccc cccggtgcag gccggtcgcg
     1561 acgtcccgat caagcgcgat cgcgcggccg agatcgagca ggcgcagcag accggcccga
     1621 agatgctcgc ctgaaggggg cggcagcatg atcgatctgt ccaaggtgcg cgcgatcgac
     1681 gtgcacgtgc atgccgatgt ctcgtgccac gatcccgagg acccggtcat gggccgcttc
     1741 tacgatgcgg cctcggcgta cttcaaggcg ccgcgcgagc ggccgaagat ccccgagatc
     1801 atcagcctgt atcgccagca gcagattgcg ttctgcctgt tcacggtcga ctgcgaaagc
     1861 agtctcggcg ccaagcgcgt cagtaactac gagatcgcgg actacgccgc gaagaacgac
     1921 gacatcgtca tcccgttcgc ctcgatcgat ccgcacaagg gcagtatggg tgcgcgcgaa
     1981 gcccgcgacc tcatcgagca tcacggtgtg cgcggcttca agttccaccc catcatgcag
     2041 gatatccacc cggccgatcg catcgcttac cccatctacg aagtgatcgc cgcgcacaag
     2101 ctgccggcga tttttcacac cgggcactcg gggatgggca cggggctgcc cggcggcggc
     2161 ggggtgcgcc tgaaatgggg ccagcccatg ctgatcgacg atgtcgccgc ggatttcccc
     2221 gacatgaaga tcgtgctcgc ccacccgagc tggccgtggg tggatgagag cctgtcgatg
     2281 gcgctgcaca aggacaacgt ctacatcgat ctgtccggct ggagcccgaa gtacttccag
     2341 ccgcagatca tccagtacgc caacgggccg ctgaagacga agatgatgtt cggctcggac
     2401 ttcccgttga ttcgcccgga caagtggctc gaggcggcgc ggcaggtcgg cttcaaggcc
     2461 gaggtcatgc ccggcatcat gaaagagaat gccgcacggc tcctggggct cacgtgagcg
     2521 agttcgccaa tcgccatgta ctcatcaccg gcgcctcgag cggcatcggg ctcgccacgg
     2581 cgcggctgct ggcgcgccgc ggtgcgcgcg tgttcctcat cgcacgcagg gacgacaggc
     2641 tcgccgcggc caccgcggcg atagcgcacg atggcggcac tgccgcctgg gcggccgccg
     2701 acgtcgccga tcgggctgcg ctgctcgcag ccatcgacaa ggccgaggag gccttcggcg
     2761 ccgttgcagg cctcttcgcc aacgctggcg gcggcggacg cttcgcaccg ctcgccgagt
     2821 acgatgatgc gcagttcgac agcttgctgc gcaccaactt gaccagcgtc tactgggtca
     2881 tgaagcgcgt gctgccgccg atgctcgccg ccggcagcgg cagcatcgtc gtcacgggca
     2941 gcctcgcgag cgcccgcggc atgccaaata acatcgccta cgtcgcctcc aaacatgggg
     3001 tgatgggact ggcgcgtgcg gcggcggccg aggccgcgcc gcgcaacgtg cgtgtcaact
     3061 gcgtgctgcc cggtctcatc gagacgccga tgctgatgca gctcgacccg ggcgcgccgg
     3121 ccgaggcgat ccgcgcgcgg ctcggtagcc acgtgccgca gggccgaatc ggcagcgcgg
     3181 atgagctggc ggagttggtt tgtttcctgc tgtccgaccg tgccagccac atcaccgcgc
     3241 agaccatcgc ggtcgatggc ggcattctcg gcactctcat accacggtag acgtccatga
     3301 cactcaagta ctatcacgcg gagccgctgg cgaactcgct gaagtcgatg gtcccgctca
     3361 tcgagaaggg tctgccctgg cagagcatct acgtcgatct gcacaaattc gagcagcacg
     3421 agccgtggtt cacggccata aacccggaag ggcaggtgcc ggtgctcgac catgacggcg
     3481 tcatcatcac ccatacgacc gtcatcaacg agtacctgga ggatgtcttt cctgatgtgc
     3541 cgctgcggcc gcgtgacccg gtgggcgcgg cgcgcatgcg ctactggaac aaattctgcg
     3601 acgagcaggt gatgaactat gtctccatgc acggctggca ccgcatggtg ggcatcatcg
     3661 cgcgcagcat cgagagcggc gagttcgacc ggctgatgga gcgcatcccg ctgccggacc
     3721 agcgcaccaa atggcgcaca gcgcgctcgg gtttcagcga gaaggacctg gcgtacgcga
     3781 ccgagaagat cctctacgcg gtggacaagg tcg
//
LOCUS       DATDMP010000289         6038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_45477, whole genome shotgun sequence.
ACCESSION   DATDMP010000289 DATDMP010000000
VERSION     DATDMP010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45477"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..218)
                     /locus_tag="VI195_04955"
     CDS             complement(66..218)
                     /locus_tag="VI195_04955"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY6823765.1"
                     /translation="MNAERQRQIASMGGKAVPDEKRSFSQHPELAAEAGRKGGQASHG
                     GATRPA"
     gene            complement(440..1492)
                     /locus_tag="VI195_04960"
     CDS             complement(440..1492)
                     /locus_tag="VI195_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823766.1"
                     /translation="MIAFVLLAAALTMASVVAVAIPLLRRDTDGAASAPWTALAASGF
                     LVVGSAALYVTWSNWPWRAAPAPDSPESMVSRLARRLERDPNDLNGWLMLGRSYTVLQ
                     EYPLAARAFGRAVQLSGAKNAEALAGEGEALALSDESELDGRAGRLIEQALVLEPSSG
                     KALFFGAAVAARRGDLPLARQRFAKLLELNPPDAIRPMIEQQIVAIDEKLSKTAGTTA
                     AVQQGQSAKRASSSPEPQGSAPLVRVNVTLAPSLASAAGAAPLFVFVRDPAQGGPPLA
                     VKRLESHFPQSVALSPADSMVPGRAFSAGQNVQVVARIARSGTPLAASGDPFGEVTYK
                     VGHDGVVSLVINRLTP"
     gene            complement(1489..1941)
                     /locus_tag="VI195_04965"
     CDS             complement(1489..1941)
                     /locus_tag="VI195_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY6823767.1"
                     /translation="MMRRAAQAVVIAAGLVLACARVAVAVDPTELPDPVLQARYVALT
                     HELRCMQCQNESLADSPVGLAADLRREVRDQLLAGRSDEQIRAWMSARYGDFILFRPR
                     YSLRNAWLWGAPGALLLIGAWVCWRVVRARTRLLPGDHEPLEEDGPSR"
     gene            complement(1938..2477)
                     /locus_tag="VI195_04970"
     CDS             complement(1938..2477)
                     /locus_tag="VI195_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY6823768.1"
                     /translation="MTRFLLPLGIFLLLAVVLAIGIRHSPEKGVIASPLLGKAAPSFA
                     LPDLTDPARAVRSADLRGHWYLFNVWGTWCGGCREEHETLLQVHRAGVVPIIGLDWKD
                     DDAQALNWLKELGNPYQSVATDHSGRQAIEWGVYGAPETFLVNPQGIVVYKHVGALTP
                     DTWTREILPRVSGSPAGRS"
     gene            complement(2474..4429)
                     /locus_tag="VI195_04975"
     CDS             complement(2474..4429)
                     /locus_tag="VI195_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY6823769.1"
                     /translation="MLPELGHFALILALLLAGLQAFFGIAGPALARDRWSRAVTAAVA
                     GQAVMVATAAACLFSSFIGEDFSVVYVAENSNSALPLFYRVTALWGAHEGSLLLWILL
                     LAGWTVAVALGSGRLPARFAARVLGVLGLVSFGFLLFTLATSNPFLRLDPPAPDGRDL
                     NPILQDPGLAVHPPILYTGYVGFAVAFAFACAAMLEGRLDQNWARWTRPWTTTAWAFL
                     TCGIALGSFWAYYELGWGGFWFWDPVENASFMPWLVGTALVHSLAVTDKRGLFKSWTL
                     LLAVLAFSLSLLGTFLVRSGVLVSVHSFAADPTRGIFILVFLIIMIGGALTLYAWRAP
                     RLRSSAGFELGARESFLLFNNVLLVIAAGTVFAGTLAPLISDALKLGTLSVGPPYFNP
                     TFMLSMLPLLALLAVGIHANWKRGRLRDSQRTLLLTCVAAAVLGCGFAFGLYARGSLL
                     TAIGATLGTWILLSSLVDPIDRWRRGLTLPRAVLGMTVAHMGLALAVIALTTVQSFTL
                     ERDVALAPGGRATVGDYEFRFDGVRAVEGPNYDAVAGTLTVTRHGVPIAVLTPQKRQY
                     WVQHQVISETAIRMHRGNNLLLALGEDLGAGRWSVRIQIRPLVSLVWIAAFIMAVGGA
                     LAASDRRYRTLRAAAESPAGATAGQPG"
     gene            complement(4434..4913)
                     /gene="ccmE"
                     /locus_tag="VI195_04980"
     CDS             complement(4434..4913)
                     /gene="ccmE"
                     /locus_tag="VI195_04980"
                     /inference="COORDINATES: protein motif:HMM:NF009729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY6823770.1"
                     /translation="MTPRRRRLALVIGIVAGVSIAGALALSAFRQNVTFFFDPSQVAA
                     GQVPSGERFRLGGMVTQGSLHRAPGSLEVHFIVTDFNHEVPVSYTGVLPDLFREGAGV
                     VAHGRLRGDGTFVADEVLAKHDEKYMPPEVARSLKRRHGDSRTEPVGPATASSGSGS"
     gene            complement(4910..5074)
                     /gene="ccmD"
                     /locus_tag="VI195_04985"
     CDS             complement(4910..5074)
                     /gene="ccmD"
                     /locus_tag="VI195_04985"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY6823771.1"
                     /translation="MRAFLDMGGYAAFVWPAYGVTLGVVLLNVFWARRALERSRAEAR
                     RRLQLQGEAS"
     gene            complement(5071..5808)
                     /locus_tag="VI195_04990"
     CDS             complement(5071..5808)
                     /locus_tag="VI195_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY6823772.1"
                     /translation="MNWTWFHRLASPPHVYTLAARLTPWFAWPAALCIAAGLWGGLVL
                     APPDYQQGDGFRIIYVHAPSAWMSLMVYVTMASAAAIGLIWRMKVAHAVAASCAPVGA
                     SFTFAALVTGALWGQPMWGTYWEWDPRLTSELVLLFLYLGYIGLRAGIEDPQRADRAS
                     ALLAVVGVVNVPIIHYSVTWWNSLHQAPSVMRLGKPAMPLAMLVPLLLMLLGFTLLFV
                     ALLLTRLRGEVLSRERAASWIREVVRT"
     gene            complement(5823..>6038)
                     /locus_tag="VI195_04995"
     CDS             complement(5823..>6038)
                     /locus_tag="VI195_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY6823773.1"
                     /translation="LTLGIKRSGALLSLLTLPLSLPVLIFGARATQLAIHGASFLAPL
                     ELLAALAVLGITLAPLAAAAAVRISLE"
BASE COUNT         1159 a         2046 c         1980 g          853 t
ORIGIN      
        1 ccccgccgcc gcctcaccat ccctggctac tcctcctccc acattcggtc gtgggtgggg
       61 gcgcctcagg ccggacgggt agcgccgccg tggctggcct gtccgccttt acggcccgcc
      121 tcagcggcca gctctggatg ctgcgagaag ctgcgtttct cgtccggcac ggccttgccg
      181 cccatgctgg cgatctggcg ctgccgctcg gcgttcatgg aggcgaaacc acggtgcgaa
      241 atgcgccctt cattcactgt tccttcagcc atcactgctc tccttcagag tcacggaccc
      301 ggtgtccccc gggtgctcaa gtgtgatctg gttcacaaaa aaggggggta tcggcgcaga
      361 gcatacgcct tgaacgtgtt atggaacatc aggggaaccc tgagcggtgc ggtcagcgcc
      421 cgcctacttt ggttgtgagt cagggtgtaa gccggtttat aaccagactg actacgccat
      481 cgtggcccac cttgtaagtt acttctccga acggatcacc gcttgcggcc agcggggtcc
      541 ctgaacgggc gattcgagcg accacctgaa cgttttgtcc ggcagagaaa gcgcgcccgg
      601 gaaccatgga gtcagccggg ctcaacgcaa cactctgagg gaaatgactc tccaagcgct
      661 tgacggccaa ggggggtccg ccctgggcag ggtcgcggac gaaaacaaac aggggcgcgg
      721 ctccggccgc cgaggcgagc gagggtgcca aagtaacatt gacccggacc agcggtgcgc
      781 ttccctgggg ctcaggggag gaagacgccc gttttgcgga ctgaccctgt tgcaccgcag
      841 cggtcgttcc cgcagttttc gacaatttct cgtcgatcgc cacaatctgt tgctcgatca
      901 tcggccggat ggcgtccggg gggttgagct cgagaagctt cgcgaagcgt tgtcgggcga
      961 gtggcaggtc gccgcggcgc gcggcgaccg ccgccccgaa gaacaacgcc ttgcccgaac
     1021 tgggctcgag cacgagcgcc tgctcgatga gccgtccggc acgtccatcg agctcgctct
     1081 catcgctgag cgccagtgcc tcaccttcac cggcgagcgc ctcggcgttc ttcgcgccgc
     1141 tcagttgaac ggcccggccg aatgcacgtg ctgcgagcgg atactcctgc aacacggtgt
     1201 atgagcgtcc gagcatcagc cagccgttga ggtcattcgg atcgcgctcg aggcggcggg
     1261 caagccgcga caccatgctt tcgggagagt cgggtgccgg tgccgcccgc catggccagt
     1321 tgctccaggt gacgtacagc gcggccgagc ccaccacgag aaacccggag gcagccagcg
     1381 ctgtccacgg agcgcttgcc gcgccgtcgg tgtcacgccg cagtaacggg atggccaccg
     1441 caacgacact ggccatggtc agtgccgcag ccagcagcac gaaggcgatc atcggctcgg
     1501 tccgtcctct tccagcggct catgatcacc ggggagcagt ctcgtccgag cgcgcacgac
     1561 gcgccagcac acccatgccc cgatcagcaa cagcgcgccc ggtgcgcccc acagccacgc
     1621 gttgcgcagc gaatagcgcg gccggaacag gatgaaatcg ccgtagcggg cggacatcca
     1681 ggcacggatc tgctcgtccg atctcccagc cagcagctga tcgcgcacct cccgacgcag
     1741 gtccgccgcc agcccgaccg gggagtccgc aagtgactca ttttgacact gcatgcagcg
     1801 cagctcgtga gtgagcgcca catagcgtgc ctgcagaacc gggtccggca gctcggtcgg
     1861 atcgacggca acagcgacgc gtgcgcaagc cagcacgagc ccggcagcga tcacgacggc
     1921 ttgcgcggcg cgcctcatca tgagcggcct gcggggctgc ccgagactct cggcaggatc
     1981 tcgcgcgtcc aggtgtcggg agttagagcg ccgacgtgct tgtaaacgac gatgccctgc
     2041 ggattcacga ggaaggtttc cggggcgccg taaacccccc actcgatggc ttgccgtccg
     2101 ctgtgatcgg tggccaccga ctgataggga ttgccgagct ccttgagcca gttgagcgcc
     2161 tgcgcgtcat cgtccttcca gtcgagtccg atgatcggca cgacgccggc gcggtgtacc
     2221 tgcagcaaag tctcatgctc ctcacgacat cccccgcacc acgtacccca gacgttgaac
     2281 aggtaccagt ggcctcggag atcggccgag cgcaccgcac gcgcgggatc ggtcagatcc
     2341 ggaagagcga atgacggggc cgctttgccg agcagtggag aggcaatcac acccttctcc
     2401 ggagaatggc ggatgccgat ggccagcacc acggcgagca gcaggaaaat cccgagtggc
     2461 agcagaaaac gtgtcatcca ggctgccccg cggtcgcccc cgccggggac tccgccgccg
     2521 cacgcagcgt gcggtagcgc cggtcggatg cggccagtgc ccctcctact gccatgatga
     2581 acgcggcaat ccagaccagg ctcacgagcg gacgtatctg tatgcgtacg ctccagcggc
     2641 cggcgccgag atcctcaccg agcgcgagca gcaggttgtt gccgcggtgc atgcggatgg
     2701 cggtctcgct gatgacctgg tgctgtaccc agtactggcg cttttgtggg gtaagcaccg
     2761 cgatcggcac gccgtggcgc gtcacggtca gggtgcccgc cacagcgtcg tagttgggac
     2821 cttcgacggc ccggacgcca tcgaagcgga actcgtagtc gccgaccgtg gctctgccac
     2881 ctggtgcgag agccacatcg cgctcaaggg tgaaggactg caccgtggtg agcgcaatga
     2941 ccgcgagcgc gagtcccatg tgcgccaccg tcattccgag caccgcacgc ggaagcgtga
     3001 ggccccgccg ccagcgatcg atcgggtcca cgagcgatga cagcaggatc catgttccga
     3061 gcgtcgcgcc gatggcggtg agcagacttc cacgcgcgta gagtccgaat gcgaagccgc
     3121 agccgagcac ggcagccgcc acgcaggtga ggagcagtgt gcgctgactg tcgcgcaacc
     3181 ttccgcgctt ccagttcgca tgtatgccca ccgcgagcag cgccagcagc ggcagcatcg
     3241 acagcatgaa ggtcggattg aagtaaggcg gaccgaccga gagcgtgccg agcttcagcg
     3301 catcggagat gagcggcgcc agggtgccgg caaagaccgt gccggcggca atgaccagca
     3361 gcacattatt aaagagcagg aacgactcgc gcgcgccgag ctcgaatccc gcgctcgagc
     3421 gcagccgcgg ggcgcgccag gcatacaaag tgagcgcgcc gccgatcatg atgatcagga
     3481 acaccagaat gaatatgccg cgcgtcggat cggcggcgaa ggaatgcacc gaaacgagta
     3541 cgcccgagcg caccaggaat gtccccagaa gactcagcga gaacgccagg accgccagca
     3601 acagtgtcca gctcttgaag agcccgcgct tatcggtcac cgccagggag tgcacgagcg
     3661 ccgtgcccac cagccacggc atgaaagagg cattctccac cgggtcccag aaccagaacc
     3721 cgccccagcc gagctcgtag taggcccaga aactgccgag cgcaatgcca caagtgagga
     3781 aggcccacgc ggtggtcgtc catggccgcg tccagcgcgc ccagttctga tcgaggcgcc
     3841 cctcgagcat tgccgcgcag gcgaaagcga aagccaccgc aaaaccgacg tagccggtgt
     3901 agaggatcgg cgggtgcact gccagacccg gatcctgcag gatcggattg aggtcgcgtc
     3961 catcgggggc cggcggatcg agccgcagga atgggttgga ggtggcgagc gtaaagagga
     4021 gaaagccgaa gctcacgagc ccgaggacgc ccagcacacg cgccgcaaag cgcgccggca
     4081 gtcggcccga gccgagtgcc acggccacgg tccacccggc gagcagcaga atccacagca
     4141 gcagcgagcc ttcgtgtgcg ccccacaagg cggtcacgcg gtagaaaagc ggcagcgcgc
     4201 tgttggaatt ctcggcgaca tataccaccg agaagtcctc cccgatgaag ctgctgaaga
     4261 ggcaggccgc tgcggtcgcg accatgaccg cctgcccggc gaccgccgcg gtcactgcgc
     4321 ggctccagcg atcgcgcgcc aaagccggtc cagcgattcc gaagaatgcc tgcaggcccg
     4381 ccagcagcag cgccagaatc agcgcgaagt gaccgagctc gggcagcatg ccctcagctt
     4441 cccgatccgg agctcgccgt ggcggggccg acgggctcgg tacgcgaatc gccgtggcgg
     4501 cgcttcaatg agcgggccac ctccggcggc atgtacttct cgtcgtgctt ggcgagcact
     4561 tcgtccgcga cgaaagtgcc atcgccgcgc aggcggccgt gcgcgacgac gcccgccccc
     4621 tcgcgaaaga ggtccggcag cacccctgtg tagctcaccg gcacctcgtg attgaagtcg
     4681 gtcacgatga agtgcacctc gagactgccc ggtgcacggt gcaggctccc ctgcgtgacc
     4741 atgccaccga gacggaagcg ctcaccgctg ggcacctgac cggcggccac ctggcttgga
     4801 tcgaaaaaaa acgtgacatt ctgacggaag gcactgaggg ccagcgcgcc ggcgatgctc
     4861 acgccggcca cgatgccgat caccaatgcg aggcggcgcc ggcgcggagt caagaagcct
     4921 ctccctgcag ctgcagacgc cgccgcgcct ccgcgcggga gcgctcgagc gcacggcgcg
     4981 cccagaagac attcagcagg accacgccga gtgtcacccc gtaagccggc cacacgaagg
     5041 ccgcgtatcc gcccatgtcg agaaaagctc tcatgtccgc accacctcgc gaatccagct
     5101 cgccgcgcgc tcgcgcgaga gcacctcgcc gcgcagccgc gtgagcagga gcgcgacgaa
     5161 cagcagcgta aagcccagca gcatcagcag cagcggcacc agcatcgcaa gcggcatggc
     5221 gggcttcccc aggcgcatca ctgaaggggc ctgatgcagt gagttccacc aggtcaccga
     5281 gtagtggatg atgggaacgt tcaccacacc gaccactgcc agcagcgcgc tcgcgcgatc
     5341 ggcgcgctgc ggatcctcga tgcccgcgcg cagcccgatg tagcccaggt acagaaacag
     5401 cagcaccagc tcggaggtca gacgcggatc ccactcccag taagtacccc acatcggctg
     5461 gccccagagc gcccccgtca ccagcgccgc gaacgtaaag gaggcgccga ccggcgcgca
     5521 gctcgccgcc accgcgtgcg ccaccttcat gcgccagatg agtccgatcg ctgcggcgct
     5581 cgccatggtc acatacacca tgagcgacat ccaggcactc ggtgcatgta cgtagatgat
     5641 gcggaagccg tcgccctgct ggtagtcggg cggagcgagc accagcccgc cccagagacc
     5701 ggcggcgatg cacagcgccg cgggccaggc gaaccaaggt gtgaggcgtg ccgcgagcgt
     5761 atagacgtgc ggtggcgatg cgagccgatg gaaccaggtc cagttcatgc gagatgagtg
     5821 ccctactcca ggctgatgcg tacggcggcc gctgcggcca gcggcgcgag cgtgatcccg
     5881 agcactgcga gcgccgcgag cagctcgagc ggtgcgagaa atgacgcgcc atggatcgca
     5941 agctgtgtcg cgcgcgctcc gaagatcagc accggcaggg acaggggcag cgtaagcagc
     6001 gacagcagtg ccccgctgcg tttgatcccg agcgtcag
//
LOCUS       DATDMP010000290         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_45745, whole genome shotgun sequence.
ACCESSION   DATDMP010000290 DATDMP010000000
VERSION     DATDMP010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45745"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VI195_05000"
     CDS             <1..356
                     /locus_tag="VI195_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823774.1"
                     /translation="GRPAPRIISRATVERMRPGSVVVDIAAEQGGNCELTRAGETVEH
                     QGVQIMGPVNLATELAYNASEMYARNLYNFLRPALVKGELVIDWNDEVFAQSCLTRAG
                     QIQHAPTAQALGSAA"
     gene            353..661
                     /locus_tag="VI195_05005"
     CDS             353..661
                     /locus_tag="VI195_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY6823775.1"
                     /translation="MTGMVALYIFMLAAFTGYEVIARVPVILHTPLMSGSNFVHGIVL
                     VGAMVALGAAHTPLEKSIGFIGVLLAAGNAVGGYVVTERMLEMFKASGARAGGDKRTS
                     "
     gene            661..2049
                     /locus_tag="VI195_05010"
     CDS             661..2049
                     /locus_tag="VI195_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY6823776.1"
                     /translation="MPGWWSADLAIQISYFITAVLFIMGLKRMSSPVTARSGILWAGA
                     GMAVATLITFLYPGMSNYALILPAIFVGGVLAAWSGRRVAMTNMPQMIALYNGMGGGA
                     AAAIAAVELYRGGTAGYASTSLAVAGGIIGAVSFSGSLIAFGKLQGLIKRSLRFAGQQ
                     ILNLLLLLAALVLGALLISQTNSTPALVTGLFLLALVLGVSMTLPIGGADMPVVISLY
                     NALTGLAVGFEGFVLNNAAMIIAGTVVGSAGTLLTQLMAKAMNRSLGNVLFSGFGDTG
                     AAASGAVQGSLKPIEASDVGVMLAFAQKVIVVPGYGMAVAQAQHKIWELCQLLIDRGV
                     QLRFAIHPVAGRMPGHMNVLLAEAGVPYDLIADLDEINAEFDTADVALIIGANDVVNP
                     VARTDKSSPIYGMPILNADKAKNVIVIKRGQGAGFSGIENALFYLDNTRMLYGDAQKV
                     AAELIQAVKAVG"
     gene            2096..>2844
                     /locus_tag="VI195_05015"
     CDS             2096..>2844
                     /locus_tag="VI195_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY6823777.1"
                     /translation="MHRRKLVETAAALPLLALMPALSAGPAAAPLQRVRPGEPGWPSA
                     ADWRKLDDAVGGALLEVRALFSACESDRGSTGCREVFANIQNPFYIGDQAGGTQVSGW
                     LDAWTPAPSAYALKARNSADVAAGVNFARERRLRLAVKGGGHSYQGTSSAPDSLLIWT
                     RALNQITLHDAFVPAGCAGKFEAAPAVSAGAGAMWIDLYDAVTSRAGRYVQGGGCTSV
                     GVAGLVQSGGFGSFSKGFGSAAAGLLEAEIVT"
BASE COUNT          438 a          950 c          982 g          474 t
ORIGIN      
        1 ccggccggcc ggcgccgcgc atcatctcgc gcgcgaccgt cgagcgcatg cggccgggct
       61 cggtggtggt cgacatcgcc gccgagcagg gcggcaactg cgagctgacg cgcgccggcg
      121 agaccgtcga gcatcagggc gtgcagatca tgggtccggt caatctcgcc accgagctcg
      181 cctacaacgc gagcgagatg tacgcgcgca acctctacaa cttcctgcgc ccggcgctgg
      241 tgaagggtga gctggtgatc gactggaacg acgaagtgtt tgcgcagtcc tgcctgacgc
      301 gcgccgggca gatccaacac gcgccgaccg cgcaagcgct ggggagtgcg gcatgaccgg
      361 catggtggcg ctctatattt tcatgctggc ggccttcacc ggctacgaag tgatcgcgcg
      421 cgtgccggtg atcctgcata ccccgctcat gtcgggatcc aacttcgtac acggcatcgt
      481 gctggtcggc gccatggtcg cgctcggcgc ggcgcacacc ccgctggaga agagcatcgg
      541 cttcatcggc gtgctgctcg cggccggcaa cgcggtcggc ggctacgtcg tgaccgagcg
      601 catgctggag atgttcaagg cgagcggggc gcgcgccggc ggcgacaaga ggacctcctg
      661 atgccggggt ggtggtcggc ggatctggcg atccagatca gttatttcat taccgcggtc
      721 ctgttcatca tggggctcaa gcgcatgagc tccccggtga ccgcgcgcag cggcatcctc
      781 tgggcgggcg ccggcatggc agtcgccacg ctgatcacct ttctctaccc gggaatgtcc
      841 aactacgcac tgatcctgcc ggccatattc gtcggcgggg tactcgctgc ctggagcggg
      901 cggcgtgtgg ccatgaccaa catgccgcag atgatcgcgc tctacaacgg catgggcggc
      961 ggtgccgcgg cggccattgc ggcggtggag ctgtatcgcg gtggcaccgc gggttatgcc
     1021 agcaccagcc tcgcggtcgc cggcggcatc atcggcgcgg tctccttcag cggcagcctc
     1081 atcgctttcg gcaagctgca gggcctgatc aagcgcagcc tgcgcttcgc cggccagcag
     1141 attctcaact tgctgctgct gctcgcggcg ctcgtgctgg gggcactgct gatctcgcaa
     1201 accaactcga cgcccgcgct ggtgaccggg ttgttcctgc tggcgctggt actcggtgtc
     1261 agcatgacgc tgcccatcgg cggcgccgac atgccggtgg tgatctccct ctacaacgcg
     1321 ctcaccgggc tggcagtcgg cttcgagggc ttcgtgctca acaacgccgc catgatcatc
     1381 gccggcaccg tggtgggctc ggccggcacg ctgctcacgc agctgatggc caaggccatg
     1441 aaccgctcgc tcggcaacgt gctgttctcc ggattcggcg acaccggagc cgcggccagc
     1501 ggcgcggtgc agggaagtct caagcccatc gaggcgagcg atgtgggcgt gatgctcgcg
     1561 ttcgcgcaga aggtcatcgt ggtgcccggc tacggcatgg cggtcgccca ggcgcagcac
     1621 aagatctggg agctgtgcca gctgctcatc gatcggggcg tacagctgcg cttcgccatc
     1681 cacccggtcg ctggacgtat gcccgggcac atgaacgtgc tgctggcgga agccggcgtc
     1741 ccctacgacc tcatcgccga tctcgatgag atcaacgccg agttcgacac ggcggacgtg
     1801 gcgctcatca tcggcgccaa cgacgtggtc aacccggtcg cgcgtaccga caaatcgagc
     1861 cccatctacg gcatgcccat cctgaatgcc gacaaggcga agaacgtcat cgtcatcaag
     1921 cgtggccagg gcgccggctt ctccggcatc gagaacgcgc tgttctatct cgacaatacc
     1981 cgcatgttgt acggcgacgc gcagaaggtc gccgcggagc tgatccaggc cgtgaaagcc
     2041 gtgggctgag cgcgccgcgc cggcgtgcgc ggcgccctgc aggaaggcag agccaatgca
     2101 tcgcagaaag ctggtcgaga ccgccgccgc actccccctg ctggcgctca tgcccgcact
     2161 ttctgccggt cctgccgccg cgccgctgca gcgggtccgt ccgggtgagc cgggctggcc
     2221 cagcgcggcc gactggcgca agctcgatga cgcggtcggt ggagccctgc tcgaggtgcg
     2281 ggcgcttttc agcgcgtgtg agagcgaccg cggcagcacc ggctgccgcg aggtgttcgc
     2341 caacatccag aatcccttct acatcggtga ccaggcaggc ggcactcagg tttccggctg
     2401 gctcgatgcc tggacaccgg cgccgagcgc gtacgcgctg aaggcacgta acagcgcgga
     2461 tgtcgccgcc ggggtcaact tcgcgcgcga gcggcgtttg cgtctcgcgg tcaaaggcgg
     2521 cggtcacagt taccagggca catcgagcgc acccgattcg ctgcttatct ggacgcgcgc
     2581 gctgaaccag atcacgctgc acgatgcctt cgtccccgca ggctgcgccg gcaagttcga
     2641 ggcggcgccg gcggtcagcg ccggcgcggg cgcgatgtgg atcgatctgt acgatgccgt
     2701 caccagccgc gcgggccgct acgtgcaggg cggcggttgc acctcggtgg gcgtcgcagg
     2761 tctcgtgcag agcggcggct tcggcagttt ctccaagggt ttcggcagcg ccgctgcggg
     2821 actgctcgag gcggagatag tgac
//
LOCUS       DATDMP010000291         3726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1156359, whole genome shotgun sequence.
ACCESSION   DATDMP010000291 DATDMP010000000
VERSION     DATDMP010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156359"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..451
                     /locus_tag="VI195_05020"
     CDS             8..451
                     /locus_tag="VI195_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6823778.1"
                     /translation="MSAEQRIRELKLELHAPSAPIANYVNAVRAGNLLFLSGKGPRGA
                     RGKVGREFTIEQGYQLAREVGLDLVAVMRAELGSLDKVRRVVKVLGMVNAEPEFGDQP
                     KVINGCSDLLVEVFGDKGKHARSAVGMGSLPGGIPVEIECIVEVD"
     gene            544..1578
                     /locus_tag="VI195_05025"
     CDS             544..1578
                     /locus_tag="VI195_05025"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6823779.1"
                     /translation="MRRAEKNLNPIGGIKAEDAREAMASLASLEPDAWAAAWSAIGER
                     YFAKGDYLEAWHVFNVARWPSEKLSPGKQKAYARALEAFHLYGRTLNPPVETLRIPFE
                     GREIVAYLRLPAARPAPLVFRINGLDSRKEELMASAEPYLKAGVAVLAIDMPGTGQAP
                     LPIEIGAERIFSRALDYLQTRPEIDAKRIVVQGVSWSGYWAALLAYEEKDRIRGAVVH
                     GVGIHEYFDTAWQKKTFSTREYLFDIYPARAAAYGARSEEEFFARGPKLSLLARGMLD
                     KPSAPMLLVNGERDSQQPIADLYLLMKHGDPKDVWVNPEGGHLGRSERWPQGKILGEV
                     IRPWVLQHLR"
     gene            complement(1571..2116)
                     /gene="orn"
                     /locus_tag="VI195_05030"
     CDS             complement(1571..2116)
                     /gene="orn"
                     /locus_tag="VI195_05030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002053300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY6823780.1"
                     /translation="MAADPNNLIWIDMEMTGLQPDSDRIIEIALLVTDSQLAPLAEGP
                     VIVVHQPDEVLDAMDSWNKGTHKKSGLIERVRASRLVEAEAERMALEFLALHVPGNTS
                     PMCGNSICQDRRFLARWMPRLEAYFHYRNLDVSTLKELVRRWKPEVLRGFAKEGRHEA
                     LADIRESIEELKFYRKSVMTI"
     gene            2135..3379
                     /locus_tag="VI195_05035"
     CDS             2135..3379
                     /locus_tag="VI195_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6823781.1"
                     /translation="MPNAFTWIFLAALAAAGATRLWLAQRQIGHVRACRDAVPPTFAD
                     TIPLEAHQKAADYTVAKTRLGMLDVLIGAALTLVLTLGGLIQWISELWAQALGPQGVL
                     HGTALLLTIFFIQAAASLPMTLYRTFVVEERFGFNRMTLRLFLIDLVKHALVGLALGV
                     PLLLTVLWLMERAGDYWWLYVWAVWVAYSLLMMMVYPAFIMPLFNKFSPLGEGELAER
                     IAALLQRTGFKSSGLFIMDGSRRSSHGNAFFTGFGAAKRIVLFDTLVNRLHPGEVEAV
                     LAHELGHYKLHHIAKGLAITIALSFAVLYVLGQVIDQEWFYQGLGVTTSGNAAALALF
                     FLVIPEFLFFLHPLTSLYSRSREFEADAYAMDYANAAELIRALVKLYHDNAATLTPDP
                     LHSAFYDSHPPAATRIARLRTQ"
     gene            3376..3705
                     /locus_tag="VI195_05040"
     CDS             3376..3705
                     /locus_tag="VI195_05040"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY6823782.1"
                     /translation="MNSLAQKKCKPCEGGVAPYTEAQAKELLRQLKGWIIEDGRLVKA
                     FPFTNYYQTMAFVNALAWISHREDHHPDMTIGYKECRIAYVTHAIGGLSENDFICAAK
                     IDALFDL"
BASE COUNT          633 a         1225 c         1200 g          668 t
ORIGIN      
        1 gtactagatg agcgcggaac aaagaatccg ggagctgaag ctcgagctgc acgcgccttc
       61 cgcgcccatc gccaattatg tgaacgcggt gcgcgccggc aacctgctct tcctctcggg
      121 caagggcccg cgcggcgcgc gcggcaaggt gggccgcgaa ttcaccatcg agcaggggta
      181 ccagctcgcg cgcgaagtag gcctggacct cgtggcggtg atgcgcgcgg agctcggcag
      241 cctcgacaag gtccgccgcg tcgtcaaggt gctcggcatg gtgaatgccg agcccgagtt
      301 cggcgaccag cccaaggtga tcaacggctg ctccgacctg ctggtcgagg tgttcggcga
      361 caaggggaag catgcgcgct cggccgtggg catgggctcg cttccgggcg gcattccggt
      421 ggagatcgaa tgcatcgtag aagtcgactg agctttctcg ctcttgcgtg ctgcctcctc
      481 tcgctcagcg cctgggcgca gagctgggga ccgcgcagcc tcgcggagct taaagaggaa
      541 accgtgcgcc gcgccgaaaa gaacctgaat ccgatcggcg gcatcaaggc cgaggacgcg
      601 cgcgaggcga tggcttcgct tgccagcctg gagcccgacg cctgggcggc cgcgtggagc
      661 gcgatcggcg agcgctattt cgccaaaggc gattacctcg aggcctggca cgtcttcaac
      721 gtggcgcgct ggccgagcga aaagctctcg ccgggcaagc agaaggcgta tgcgcgcgcc
      781 ctggaggcct ttcacctcta cgggcggacg ctcaatcctc cggtggagac gctgcgcatc
      841 ccgttcgaag ggcgcgagat cgtcgcatac ctgcggctgc cggccgcgag gcccgcgccg
      901 ctcgtgttcc gcatcaacgg actggactca cgcaaggaag aactgatggc gagcgcggag
      961 ccctacctca aggccggcgt agcggtgctc gcgatcgaca tgccgggcac cggccaggcg
     1021 cctttgccga tcgaaatcgg cgccgagcgg atcttctcgc gcgccctgga ttatctgcag
     1081 acccggcccg aaatcgacgc caagcgcatc gtggtgcagg gcgtaagctg gagcggctac
     1141 tgggccgcgc tcctcgccta cgaggagaag gaccgcatcc gcggggccgt ggtgcacggg
     1201 gtgggcatcc acgagtactt cgacaccgcg tggcagaaga aaaccttcag cacgcgcgag
     1261 taccttttcg acatctatcc ggcgcgcgcc gcggcgtacg gcgcccggag cgaagaagag
     1321 ttcttcgcgc gcgggccgaa gctctcgctg cttgcgcgcg gcatgctgga caagccctcg
     1381 gcgccgatgc tgctcgtgaa cggcgagcgc gacagccagc agccgatcgc ggatctctat
     1441 ctcctcatga agcacggcga tccgaaggat gtatgggtca accctgaggg cggccacctc
     1501 ggccgctcgg agcgctggcc gcagggcaag atcctcggcg aagtgatccg gccctgggtg
     1561 ctgcagcacc tcagatagtc attacgctct tgcggtagaa cttcagctcc tcgatcgact
     1621 cgcggatgtc ggcaagcgcc tcgtgcctgc cctccttcgc gaagccgcgc agcacctcgg
     1681 gcttccagcg gcgcacgagc tccttcagcg tcgacacgtc gaggtttcgg tagtggaaat
     1741 aggcttccag gcgcggcatc cagcgcgcga ggaaacgccg gtcctggcag atggagttgc
     1801 cgcacatcgg ggaggtgttt cccgggacgt gcagggcgag gaattcgagc gccatgcgct
     1861 cggcctcggc ttcgacgagc ctcgaggcgc gcacgcgctc gatgaggcca gacttcttgt
     1921 gcgtgccctt gttccatgaa tccatcgcat cgagcacctc gtcgggctgg tgcaccacga
     1981 tgaccggacc ctcggcgagc ggggcgagct gcgaatcggt cacgagcagc gcgatctcga
     2041 tgatccggtc ggaatccggc tgcaggccgg tcatttccat gtcgatccag atcaggttgt
     2101 taggatcggc agccatgctt tattctctca tggcgtgcca aacgccttca cctggatctt
     2161 cctcgccgcg ttggctgcgg ccggcgcgac gcgcctgtgg ctcgcgcagc ggcagatcgg
     2221 ccacgtgcgc gcgtgccgcg acgcagtgcc gccgaccttt gccgacacca tcccgctcga
     2281 agcgcaccag aaggcggcgg attacacggt agccaagacg cggctcggga tgctggatgt
     2341 gctgatcggc gccgcgctga cgctcgtgct caccctgggc ggcctgatcc agtggatctc
     2401 cgagctctgg gcgcaggcgc tggggcccca aggcgtgctg cacggcaccg cgctgctgct
     2461 tacgattttc ttcatccagg ccgccgccag cctgcccatg acgctttacc ggacgttcgt
     2521 cgtcgaggag cgcttcggct tcaaccgcat gacgctgcgg ctcttcctga tcgacctcgt
     2581 caagcacgcg ctggtgggac tcgcgctcgg cgtgccccta cttctcaccg tgctttggct
     2641 gatggagcgt gccggcgact attggtggct ttacgtttgg gcagtctggg tcgcttattc
     2701 gctcctcatg atgatggtgt atccggcttt catcatgccg ctattcaaca agttctcgcc
     2761 gctcggcgag ggcgagctcg ccgagcgcat cgccgcgctg ctgcaacgca ccggcttcaa
     2821 gtcgagcggc cttttcatca tggacggctc gcgacgctcg agccacggca acgccttctt
     2881 caccgggttc ggcgcggcga agcggatcgt gctcttcgat accctggtga accgcctgca
     2941 tccgggcgaa gtggaagcgg tgctcgcgca cgagctcggg cactacaagc tgcatcacat
     3001 cgcgaagggc ctcgccatca ccatcgccct gagcttcgcg gtgctgtacg tgctcgggca
     3061 ggtcatcgac caggagtggt tctaccaggg cctcggcgtc acgacctccg gcaacgctgc
     3121 ggcgctcgcg cttttcttcc tcgtcattcc ggagttcctt ttcttcctgc acccgctcac
     3181 cagcctctat tcacgctcgc gcgaattcga agccgacgcc tacgccatgg attacgccaa
     3241 cgccgccgag ctgatccggg cgctcgtcaa gctctaccac gacaacgcgg cgacgcttac
     3301 gcccgatccg ctgcattccg cgttctacga ttcgcatccg ccggccgcga cgcgcatcgc
     3361 ccgattgagg acccaatgaa ttccctcgct cagaagaagt gcaagccctg cgaaggcggc
     3421 gtcgcgccgt acaccgaggc gcaggcgaag gagctgctcc gccagctcaa gggctggatc
     3481 atcgaggacg gcaggctcgt gaaggcgttt ccgttcacca actattacca gaccatggcg
     3541 ttcgtcaacg cgctggcatg gatctcccac cgcgaagacc atcaccccga tatgacaatt
     3601 gggtacaagg aatgccggat tgcttatgtg acgcacgcga tcggcggctt gtcggaaaac
     3661 gatttcattt gcgcagcgaa aatcgatgcg ctattcgatc tttagcttcg cgcagagacg
     3721 aagatg
//
LOCUS       DATDMP010000292         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_490009, whole genome shotgun sequence.
ACCESSION   DATDMP010000292 DATDMP010000000
VERSION     DATDMP010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490009"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VI195_05045"
     CDS             <1..230
                     /locus_tag="VI195_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823783.1"
                     /translation="SVRTLDLYKLPGLSETIEWGRALLALDRRALDAQTVDDTLGVLL
                     KYQDDLLRVRGSEAARLLATITAEAAGAGAG"
     gene            233..1459
                     /locus_tag="VI195_05050"
     CDS             233..1459
                     /locus_tag="VI195_05050"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6823784.1"
                     /translation="MLPSPPLQPDAGQLAGNVLHFARVLRKAGLPVGTDRPLLALKAL
                     ELAGLGSRADLYAVLRACLIDRIEHRALFDQAFHAFWRDPELLAEILQLLLPRAPAAG
                     RAPGRGPSQRLLEALAPAAARVPTRRSAERVRVTPEGQPDSSDRERLRKADFDSMTTA
                     EWSAARRLIAALAPLLARLATRRHSPAARGRELDLRELLRDCARSGGEIVCLPRRRPA
                     TRIEPLTVVVDISGSMARYSRTFLHFIHALVNGSSGAALRVNAFVFGTRLTNVTRQLR
                     SRDPDEALARVVARVADWSGGTRIGECLGEFNRRWARRIPLASSTVLLLTDGLEHADT
                     GQLAIEAARLARSCRRLYWLNPLLRYDAFEPRARGIRAILPHVDRLLPIHNLASLEEL
                     TRVLTAAYQGEPRAWK"
     gene            1450..1998
                     /locus_tag="VI195_05055"
     CDS             1450..1998
                     /locus_tag="VI195_05055"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY6823785.1"
                     /translation="MEMKGSRVLPAGREVAWGLLNDSEVLRQCIPGCESLTGSGPNAY
                     DVVMNAAIGPVKARFKGKMTLADIEPPVRYRLLFEGQSAQAGFARGEARVELEELSPR
                     ETRLSYTATSQIGGKLAQIGARLIDAAAGATADRFFQSFAAQLVARTQGAAGGAAAAA
                     VPPAKFGFWAWLRSFLAHLFAR"
     gene            complement(2156..>2345)
                     /locus_tag="VI195_05060"
     CDS             complement(2156..>2345)
                     /locus_tag="VI195_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823786.1"
                     /translation="GFAYQLGNLAMARMGPFQAGIAEAHGNDYASILAWTMAVVALAL
                     LLATALGREAHSVELRVS"
BASE COUNT          302 a          861 c          845 g          337 t
ORIGIN      
        1 agagcgtgcg tacgctcgac ctgtacaagc tgccggggct ctccgagacc atcgagtggg
       61 gacgcgcgct gctcgcgctc gaccgccgcg cgctcgatgc gcagaccgtc gacgacaccc
      121 tcggcgtgct gctgaagtac caggacgatc tgctgcgcgt gcgcggctcg gaggcggccc
      181 ggctgctggc aacgatcacg gccgaggcgg ccggcgccgg ggcaggctga gcgtgctgcc
      241 atccccgcca ctgcagccgg acgccggcca gctggccggc aacgtgctgc actttgcgcg
      301 ggtgctgcgc aaggccgggc tgcccgtcgg caccgatcgt ccgctgctcg cgctcaaggc
      361 gctcgagctc gcgggcctcg gctcccgtgc cgacctgtac gccgtgctgc gcgcctgcct
      421 catcgaccgc atcgagcacc gtgcgctttt cgatcaggcg tttcatgcct tctggaggga
      481 cccggagctg ttggcggaga tcctgcagct gctgctgccg cgcgcgccgg cggccggccg
      541 cgcgccgggc cgcggcccga gccagcggct gctcgaggca ctcgcccccg ctgcggcccg
      601 ggtgccgacg cgccggtccg cggagcgcgt gcgcgtgacc cccgaggggc agccggactc
      661 gtccgaccgc gagcggctgc gcaaggccga cttcgattcc atgacgacgg cggagtggtc
      721 ggcggcacgg cggctgatcg ccgcgctcgc cccgctgctc gcgcggctcg ccacccgccg
      781 ccacagcccc gccgctcgcg gacgggagct cgacctgcgg gaactgctgc gcgattgcgc
      841 ccgcagcggc ggggagatcg tgtgcctgcc gcggcgcagg ccggccacgc gcatcgagcc
      901 gctgaccgtc gtggtcgata tctccggctc gatggcgcgc tactcgcgga cgttcctgca
      961 tttcatccat gcgctcgtga acggctcgtc cggggcggcg ctgcgcgtca acgccttcgt
     1021 gttcggtacg cgcctcacca acgtcacccg gcagctgcgc tcgcgcgatc cggacgaggc
     1081 actggcacgc gtggtcgccc gggtcgccga ctggtcgggc ggcacgcgca tcggcgagtg
     1141 tctcggggaa ttcaaccggc gctgggcgcg gcgcatcccg ctcgcgagct ccaccgtgct
     1201 gctgctcacc gacggcctcg agcacgccga taccgggcag ctggcgatcg aggccgcgcg
     1261 tctggcacgc tcctgccgcc ggctgtactg gctgaacccg ctgctgcgct acgatgcgtt
     1321 cgagccgcgg gcgcgcggca tccgggcgat cctgccgcac gtagaccggc tgctgccgat
     1381 tcataacctc gcgtcgctgg aagagctgac gcgggtgctc accgccgctt accaggggga
     1441 gccgcgcgca tggaaatgaa gggttcgcgg gtgctgccgg ccggccgcga ggtcgcctgg
     1501 ggactgctca acgacagcga ggtcctgcgg cagtgcattc cgggctgcga gagcctgacg
     1561 ggcagcggcc cgaatgccta cgacgtggtg atgaatgccg ccatcggtcc ggtcaaggca
     1621 cgcttcaagg ggaagatgac cctcgccgac atcgagccgc cggtccgtta ccggctgctg
     1681 ttcgaggggc agagcgccca ggcgggattt gcccgcggcg aagcccgcgt cgagctcgag
     1741 gagctttccc cccgcgagac ccggctgagc tacaccgcca cctcccagat cggcggcaag
     1801 ctcgcgcaga tcggcgcacg gctgatcgac gcggcggcgg gcgcgaccgc cgaccggttc
     1861 ttccagtcct tcgccgcaca gctcgtggcg cgcacccagg gcgcggcggg cggtgcggcc
     1921 gctgcggcgg tgccgcccgc caagttcggc ttctgggcct ggctgcgctc gttcctcgcg
     1981 cacctgttcg cgcgatagat aagggccccg gcgcgggggc gcgcgccgct gccggcggcg
     2041 gcccttcagc gcgagaacac gacgccgtgc gcattggccg atcccttcca gccgtgcggc
     2101 caggcgcgat cgtcgaaact gaagccgggc ttgccgtctg caggaattgc cgtactcagc
     2161 tgacgcgcag ctccaccgag tgtgcctcgc gaccgagcgc ggtggccagc agcagcgcga
     2221 gggccaccac cgccatcgtc caggcgagga tcgacgcata gtcgttgccg tgcgcctcgg
     2281 caatgccggc ctgaaagggt cccatgcgcg ccatcgccag gttgccgagc tggtaggcga
     2341 agccc
//
LOCUS       DATDMP010000293         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_490033, whole genome shotgun sequence.
ACCESSION   DATDMP010000293 DATDMP010000000
VERSION     DATDMP010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490033"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VI195_05065"
     CDS             complement(<1..289)
                     /locus_tag="VI195_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6823787.1"
                     /translation="MNQPAKRKARILVVDDDPGLLRLLTIRLRAESYDVEAVESAGAA
                     LAAASRFRPDLVITDLRMDQMDGIGLLKELQNRWPGLKVIILTAHGTIPDAV"
     gene            complement(319..966)
                     /locus_tag="VI195_05070"
     CDS             complement(319..966)
                     /locus_tag="VI195_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823788.1"
                     /translation="MRTRPERCLAHVTVAAAMLLAGDGCAGGFGFLSGARGENAPTAV
                     RPVSSESGLLSQYLQLLQRLVQGTASEQAEIVAAAQSDYDTAPTPSKELKLALILATP
                     GHGATNLPRAQGLLRELMAHPETLLPGERALAFLELSQIDDHLTLVSENRRLQTEAAS
                     EQQNLAASSQHRLQQEQDENARLRKELEEAKAKLDAIANIERSLNERKPNTNPRP"
     gene            complement(950..>2377)
                     /locus_tag="VI195_05075"
     CDS             complement(950..>2377)
                     /locus_tag="VI195_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6823789.1"
                     /translation="GLMLVGLALIAVPLLVAILTAVLQIRTLADTGHRIIVEGVTDAR
                     ASQALFAQIAFLERTARLYDVLNDPKLLDAYRTQDERLTATRDELGRHATAAVRDTLA
                     ALGALQASIRDRVLTTPAGAPAAATSDLSARFTQLSSLVDRVAQQSNAQVDARVAELE
                     AQTQHARQQLLWQSALLVPLALVAVLMLTVGIGRPLRQLDRAISELGEGTFSNTIAVV
                     GPHDLERLGRQLEWLRQRLLELAHERNRFLRHMSHELKTPLANIREGTELLMDGAVGE
                     LDTNQREVTAILRENGIKLQRMIENLLSFSAWQTSSVGLEPSEFRLRPLVKQVLENQQ
                     LTLLSQRVRLDVRIEDVTLVADRGKIRLILENLVSNAVKYSPKGGTIHLKARAAGAHL
                     VLDVADSGPGIPAEDRAHIFEAFYTGRAARSTAVKGTGIGLSVVLEFVSAHGGTVQII
                     DGEYPGAHFRITMPIRVSKAHAHAA"
BASE COUNT          325 a          784 c          888 g          380 t
ORIGIN      
        1 gcacggcgtc gggaatggtg ccgtgcgcgg tgaggatgat gaccttgagt cccggccagc
       61 ggttctgcag ctccttcagg agcccgatgc cgtccatctg gtccatgcgc aggtcggtga
      121 tgacgagatc cggccggaag cggctcgcgg cggcgagcgc ggcgccggcg ctctcgacgg
      181 cctcgacgtc gtagctctcc gcccgcaggc ggatggtgag caggcgcagc agccccgggt
      241 cgtcgtcgac gaccaggatc cgcgccttgc gtttggcggg ctggttcaaa gtcgtttccc
      301 tgtcagcccg cgcactgtct aagggcgcgg gttggtgttc ggcttgcgct cattcaatga
      361 gcgctcgatg ttggcgatcg catcgagctt ggccttcgcc tcctcgagct ccttgcgcag
      421 ccgggcattc tcgtcctgct cctgctgcag acggtgctgc gagctcgccg cgaggttctg
      481 ctgctcgctc gccgcctcgg tctgaagcct gcgattttct gaaaccagcg tcaaatggtc
      541 gtcaatttgc gaaagctcga ggaaggcgag cgcccgctcg cccgggagga gcgtctccgg
      601 gtgggccatg agctcgcgca gcagcccctg ggcccggggc aggttggtcg ccccgtggcc
      661 gggggtggcg aggatgaggg cgagcttgag ctccttgctc ggggtggggg cggtgtcgta
      721 gtcgctctgg gcggcggcga cgatctccgc ctgctcggag gcggttccct ggaccaggcg
      781 ctgcaggagc tgcaggtact gcgacagcag ccccgactcg ctcgacaccg gacggacggc
      841 ggtcggcgcg ttctcgccgc gggcgccgga gaggaagccg aagcccccgg cgcagccatc
      901 gcccgcgagc agcatggcgg ccgcgaccgt gacgtgcgcc aggcagcgtt caggccgcgt
      961 gcgcatgcgc cttcgacacc cggatcggca tggtgatgcg gaagtgcgca cccggatatt
     1021 ccccgtcgat gatctgcacc gtgccgccgt gggcggagac gaactccagc accaccgaga
     1081 gcccgatgcc ggttcccttg accgcggtgc tgcgcgcggc ccggccggtg tagaaggcct
     1141 cgaagatgtg ggcgcggtcc tccgccggga tgcccgggcc gctgtcggcg acgtcgagca
     1201 ccaggtgcgc acccgcggcg cgcgccttga ggtggatggt gccgcccttg ggcgagtact
     1261 tgacggcgtt cgacaccagg ttctcgagga tcaggcggat cttgccgcga tcggccacca
     1321 gcgtgacgtc ctcgatgcgc acgtcgagcc ggacgcgctg cgagaggagc gtcaactgct
     1381 ggttctcgag cacctgcttc accagcgggc gcagccggaa ctcgctcggc tcgaggccga
     1441 cgctggaagt ctgccaggcg ctgaaggaga ggagattctc gatcatgcgc tgcagcttga
     1501 tgccgttctc gcgcaggatc gcggtcacct cgcgctggtt ggtatcgagc tcaccgaccg
     1561 ccccgtccat gaggagctcc gtcccctcgc ggatgttggc gagcggggtc ttgagctcgt
     1621 gcgacatgtg ccgcaggaag cggttgcgct cgtgggcgag ctcgagcagc cgctggcgca
     1681 gccactcgag ctggcggccg aggcgctcga gatcgtgcgg gcccaccacc gcgatggtgt
     1741 tgctgaaagt gccctcgccg agctcgctga tggcgcggtc gagctggcgc agcgggcggc
     1801 cgattcccac ggtgagcatg agcacggcca ccagcgcgag cggcaccagc agcgccgact
     1861 gccacagcag ctgctgccgc gcgtgctgcg tctgcgcctc gagctcggcg accctggcgt
     1921 cgacctgggc gttgctctgc tgcgcgaccc gatcgacgag cgatgagagc tgggtgaagc
     1981 gcgcggagag atcggaggtg gcggcggccg gcgcgcccgc gggagtggtg agcacccggt
     2041 cgcggatgct cgcctgcagc gccccgagcg cggcgagcgt atcgcgcacg gcggcggtcg
     2101 cgtggcgccc gagctcgtcg cgggtcgccg tcaggcgctc gtcctgggtg cgataggcgt
     2161 cgaggagctt cgggtcgttc agcacgtcgt agaggcgcgc cgtgcgctcg aggaaggcga
     2221 tctgggcgaa gagcgcctgg ctcgcccgcg catccgtcac cccctcgacg atgatccggt
     2281 ggccggtgtc ggccagggtg cggatctgaa gcaccgcggt caggatggcg accaggagcg
     2341 gcacggcgat cagcgccagg ccgaccagca tcaggcc
//
LOCUS       DATDMP010000294         3495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2157342, whole genome shotgun sequence.
ACCESSION   DATDMP010000294 DATDMP010000000
VERSION     DATDMP010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157342"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VI195_05080"
     CDS             complement(<1..206)
                     /locus_tag="VI195_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823790.1"
                     /translation="MSASPLQPLVIWFGRVGDMILLSALLEILHRRYGSRCHLIGAGS
                     WPAQLYAAHADVARVTCLHRYTAFL"
     gene            282..1166
                     /locus_tag="VI195_05085"
     CDS             282..1166
                     /locus_tag="VI195_05085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY6823791.1"
                     /translation="MRAFAWFLGLMAAALAAVALLAWPAWMLVHPHFDFPFHRIAERL
                     GMLALLLGFLALARHLGLTDRASLGYGAARRLFIPELLIALLLGIASMLAVVGLMAAL
                     GLLEWRNGSAGAHLAKLLVSSALSGFAVAFIEETFLRGAMHTAIERESGTPAAVLFTA
                     LLYAATHFFAKFHIPPEQVNRHSGIDLLVGTLYAFAHPVAIADAFLALAAVGVVLGLV
                     RAATGNIAACIGLHAGWVWVMLVTHELAQPRAGAALGFLLSRFDGFVGWLVLGWTLLL
                     AVPLWRFYAARAAGAAGS"
     gene            complement(1207..1992)
                     /locus_tag="VI195_05090"
     CDS             complement(1207..1992)
                     /locus_tag="VI195_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY6823792.1"
                     /translation="MRGSHNVVYLAGSIHMLPANDAALPAGFTRAYADSTTLVMELDL
                     GKFDPMEAMSWMMDHGTLPAGTTLRGLLGEQRYGRVSGAAASVGMPMTGLDSLAPWVV
                     GIEITDAAYEHEGFDPDQGVEEQLLRLAQADHQATAGLETLSEELGSLSSLSSADQIR
                     MLDQTVDELKDLKSEMREVTGAWRSGDGGRLAALLASEYNAFPSLYKPLVTERNRRWL
                     PQVEQLLKSKDNTLVIVGALHLVGEGGLLELLRRKGYSITQLS"
     gene            complement(2022..2813)
                     /locus_tag="VI195_05095"
     CDS             complement(2022..2813)
                     /locus_tag="VI195_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY6823793.1"
                     /translation="MSVLRLIQFTDPHLYGSENESLRGVPTLPALRAALAHARARDWP
                     PDALLVTGDIVQDDAGGYASFREVLGGLGLPVLCLPGNHDEPEAMRRELARAPFVLDE
                     FLDLGRWRIVLLDSCIPGAASGALSAPSLAALERALATAGTRHCMVCLHHHPVPMASR
                     WLDRVGLTNAAEFLHAIDAHRNVRAIVWGHVHQSYDGLRKGVRLLATPSTCAQFLPNS
                     DEFAVDPRPPAYRTFELRADGSLLTEVVWVDQHCGGSSDSAYSAA"
     gene            3205..>3495
                     /locus_tag="VI195_05100"
     CDS             3205..>3495
                     /locus_tag="VI195_05100"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY6823794.1"
                     /translation="MTTRRELANAIRALSMDAVQRANSGHPGMPMGMADIAQVLWSDF
                     MRYNPANPLWENRDRFVLSNGHGSMLLYSVLYLTGYSMTLEDIKSFRQLGSRT"
BASE COUNT          549 a         1185 c         1186 g          575 t
ORIGIN      
        1 agaaacgcgg tataacggtg caggcaggtg acgcgcgcca cgtcggcgtg cgcggcgtag
       61 agctgcgcgg gccaggagcc ggccccgatc aggtgacagc ggctgccgta gcggcggtgc
      121 aggatttcca gcagcgccga cagcaggatc atatcgccga cccggccgaa ccagatcacg
      181 agcggttgca ggggagaggc cgacatgact cctcacgagt gcgcgagcac cataagctat
      241 gcggacgggg cgcggcgagg caagcccgct cccgttgcta tatgcgtgca ttcgcgtggt
      301 ttctcggact catggcggcg gcgctggcgg ccgtggcgtt gctcgcctgg ccggcgtgga
      361 tgctggtgca tccacatttc gactttccgt tccaccgcat cgccgaacgc ctcggcatgc
      421 tggcgctgct gctaggcttt ctcgcgctcg cccgccacct tgggctgacc gaccgggcaa
      481 gtctcggcta cggcgccgcc aggcgcctct tcatacccga gctgctcatc gcgctgttgt
      541 taggcatcgc cagcatgctc gcggtcgtgg ggctgatggc cgccctcggg ctgctcgaat
      601 ggcgcaacgg gtccgccggc gcacacctcg ccaagctcct cgtgagctcc gccctgagcg
      661 gctttgccgt ggcattcatc gaggagacct tcctgcgcgg ggcgatgcac acggccatcg
      721 agcgcgagtc cggcacgccc gcggcggtgc tgttcaccgc gctgctgtac gcggcaacgc
      781 atttcttcgc gaagttccac attccgcccg agcaggtcaa ccggcacagc ggcatcgacc
      841 tgctcgtcgg cacgctctac gcctttgccc atccggtcgc gatcgcggat gcattcctcg
      901 cgctcgccgc ggtcggcgtg gtgctcggcc tggtgcgtgc agcgaccggc aacatcgcgg
      961 cgtgcatcgg gctgcacgcc ggctgggtgt gggtgatgct ggtgacgcac gagcttgcgc
     1021 agccgcgtgc gggtgccgcg cttggctttc tgctcagccg cttcgatggt ttcgtcggct
     1081 ggctggtcct tggctggacg ctactgctcg ccgtgccgct gtggcgcttc tatgctgcgc
     1141 gcgcagccgg cgcagccgga agctgatgtc cgccaagatc gcttcctgcg atttcggcca
     1201 atccgcctaa ctgagttgcg tgatgctgta gcccttgcgg cgcaacagct cgagcagacc
     1261 cccctcgccg accaggtgca gcgccccgac tatcacgagc gtgttgtctt ttgacttgag
     1321 cagctgctcc acttgcggga gccagcgccg gttgcgctcg gtcaccagcg gtttgtagag
     1381 cgaggggaag gcgttgtatt cactcgcgag cagcgcagcg aggcggccgc catctccgct
     1441 gcgccaggca ccggtgacct cgcgcatctc ggacttgaga tccttaagct catcgacggt
     1501 ctgatcgagc atgcggatct ggtcggcgct cgagagtgag ctgaggctgc cgagctcctc
     1561 ggacaacgtt tccaggcccg cggtcgcctg gtggtccgcc tgtgcgaggc gcagcagttg
     1621 ctcctcgacg ccctggtcgg gatcgaagcc ctcgtgctcg taggcggcat cggtgatctc
     1681 gatgccaacg acccagggcg caagcgaatc gagaccggtc atgggcatgc cgacactcgc
     1741 agccgcgccg ctgacgcgcc cgtagcgctg ctcccccagc aggccgcgca gcgtggtgcc
     1801 ggccggcagc gtgccgtggt ccatcatcca gctcattgcc tccatcggat cgaacttgcc
     1861 aaggtcgagc tccatgacca gcgtggtgga atcggcgtag gcgcgggtga atccggccgg
     1921 cagcgcggcg tcattcgccg gcagcatgtg gatcgagccg gcgaggtaga cgacgttatg
     1981 tgagccgcgc accgcccaga ccgggctcgc ggcctgcgcg ctcaggcagc cgagtaggcc
     2041 gaatccgagg agccaccgca gtgttgatcg acccacacga cctccgtcag cagcgagcca
     2101 tccgcccgca gctcgaacgt acgatatgcc ggcggtcgcg ggtccaccgc gaactcgtcc
     2161 gagttgggca ggaactgagc acacgtcgaa ggggtcgcga gcagccgcac tcccttgcgc
     2221 agtccatcgt agctctggtg cacatgaccc cagacgatcg cacgcacgtt gcgatgtgca
     2281 tcgatggcgt gcaggaactc ggcggcgttc gtaaggccga cacgatcgag ccagcggctc
     2341 gccatcggta ccgggtgatg gtgcagacaa accatgcagt ggcgcgtgcc ggcggtcgcg
     2401 agtgcacgct cgagcgcggc gaggctcggc gcactgagcg cgccgctcgc ggctcccggg
     2461 atgcagctgt cgagcagcac gatgcgccag cggccgagat cgaggaactc atcgagcacg
     2521 aagggcgcgc gcgcgagctc gcggcgcatg gcttcgggct cgtcatggtt gccgggcagg
     2581 cacaggaccg gcaggccgag gccgccgagc acctcgcgaa agctggcgta tccgcccgca
     2641 tcgtcctgca cgatatcgcc ggtcaccagc agcgcatcgg gcggccagtc acgtgcgcgc
     2701 gcatgggcaa gcgctgccct gagcgcgggc agcgtcggga ccccgcgcag cgactcgttc
     2761 tcgctgccgt aaaggtgcgg gtcggtgaac tggatgaggc gcagcacgct catgaatgca
     2821 gcctagcaga gccgcgctgt tcccggcagt gaaccagtcg tgcgtggcgt gccgcggacg
     2881 acacacctgg cgcacgcttc atctgcgccc cgtttgcaga atgtgaagca gtgcccgcgc
     2941 cggcgcgccg ccagccggaa cttaagtcgc gtggcgcgcc acggcgacag tgtgcgcagc
     3001 tcacggtgcg gacagccgct cacggtgcgg acagccgctc acggtgcgga caagcgtgcg
     3061 gcacgtcgca gccgccacgc tcgcactgtg atttgagcaa cgggcgggac tgatggacaa
     3121 tgcgcgcccc tgcagtgcgc ggctgcctga ccgcagacgc ggcactagcc cgtgaagacg
     3181 gccaccggcc gcccagaggc ccgcatgact acacgccgcg aactcgccaa tgccatacgt
     3241 gcgctctcca tggacgccgt gcagcgcgcc aactccggac accccggcat gccgatgggg
     3301 atggccgaca ttgcgcaggt gctctggtcg gacttcatgc gctacaaccc ggcgaacccg
     3361 ctgtgggaga accgcgaccg cttcgtgctc tcgaacgggc atggctcgat gctgctgtac
     3421 tccgtgctgt acctcaccgg atattcgatg accctggagg acatcaaaag tttccgtcag
     3481 ctcggcagcc gcacg
//
LOCUS       DATDMP010000295         3646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_490492, whole genome shotgun sequence.
ACCESSION   DATDMP010000295 DATDMP010000000
VERSION     DATDMP010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490492"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..923)
                     /locus_tag="VI195_05105"
     CDS             complement(57..923)
                     /locus_tag="VI195_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823795.1"
                     /translation="MSLSGQTLFITGASRGIGLAIALRAARDGANIAIAAKTAEPHPK
                     LAGTIYTAAQEIEQAGGHALPLAVDVRDEAAVLAAARQCAQHFGGIDICINNASAINL
                     GSIQQIDMRRYDLIQQINTRGTFLTSRACLPYLQKSANPHILTLSPPLDLRPKWFAGH
                     LAYSLSKYGMSLCMLGLAEEYRTAGIACNALWPRTTIATAAVEFALGGVEMMRRSRKP
                     QIVADAAQVILTRPARECTGQFFIDDTVLYEAGVRDFTPYSVVPGGPLLGDLFIDATS
                     TAPPGVQVEYMR"
     gene            complement(969..1382)
                     /locus_tag="VI195_05110"
     CDS             complement(969..1382)
                     /locus_tag="VI195_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6823796.1"
                     /translation="MAAHPFAVQGIDHIVLRVEQLQRSLAFYCDVLGCKVEREQPELG
                     LTQLRAGRSLIDLVTLDGPLGAGAPPGAGRNLEHFCLTIAPFEESALIAWLVARGVLV
                     REPGSRYGAQGEGRSFYIEDPDGNRVELKAAMPGG"
     gene            1459..2310
                     /locus_tag="VI195_05115"
     CDS             1459..2310
                     /locus_tag="VI195_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY6823797.1"
                     /translation="MRWDDLRESTNVEDVRASTGGGAGLRLGVGGTLLALAASYFLGI
                     DPRLLLGLMSAVPTQQSAPAAHYGTPQDEQGRFIAAVLGETEDTWSAIFQESGREYIP
                     PKLVLYRDRMPTACGTGRAAAGPFYCPLDRKVYLDMSFFQQLSDEFQAPGQFAQAYVL
                     AHEVGHHVQNLLGIAEQVRSAQERTSQAQANQLSVKLELQADCFAGVWARHADETKQI
                     LEQGDVESALRAASAVGDDTLQRRAQGYVVPESFTHGTSAERVSWFKRGMAAGSIDSC
                     NTFSTPT"
     gene            complement(2455..2772)
                     /locus_tag="VI195_05120"
     CDS             complement(2455..2772)
                     /locus_tag="VI195_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823798.1"
                     /translation="MRRSHSLAWLSAGLLASAVGHVSGAPAAAAEVTELREGPGRDLT
                     VGRCIICHSVEYIPSNAPAMNRAAWQKTIQKMKDRFGAPITDEEAKQILDYLDAHYSG
                     KSG"
     gene            complement(2772..>3646)
                     /locus_tag="VI195_05125"
     CDS             complement(2772..>3646)
                     /locus_tag="VI195_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEY6823799.1"
                     /translation="RELQRGFEKVSVPAVNQCSGNRRGLFSPRVAGVQWGNGAIGNAL
                     WTGVRLRDVLRKAGVAADALEVVFNGADGPVLPGTPDFVKSLPIDRALDESTLIAFEM
                     NGQPLPHWNGAPARLVVPGWTGTYWMKHLTEIRVQAAAFDGFWMKSAYRIPTGAFATA
                     RFVSQETPENTPITEMVINSLIMSPAPSTRVRRGEPLHVGGKAWDAGAGIAAVEVSVD
                     GRQSWREAKLGRDLGRFAWREFQLPLDTSRSGPIEIAVRARSRNGSTQPEKLTPNPAG
                     YHDNIVQTAHVEVS"
BASE COUNT          618 a         1219 c         1209 g          600 t
ORIGIN      
        1 gaagtcctcg ggtaccggcg ctccctgcgg acggcgcgcc agcacgatac ggcggttcat
       61 cgcatgtact ccacctgcac ccccggcggc gcagtcgatg tggcgtcgat gaacaggtcc
      121 ccgagcagtg ggccgccggg caccacactg tagggagtga aatcacggac acctgcttcg
      181 tacaacacgg tgtcgtcgat gaagaactgc ccggtgcact cgcgcgccgg ccgggtgagg
      241 atcacctggg cggcgtcggc gacgatctgc ggttttctcg agcggcgcat catctccact
      301 ccgccgagcg cgaactcgac ggccgccgtg gcgatcgtgg tccgcggcca cagcgcgttg
      361 caggcgattc cggccgtgcg gtattcctcg gccaggccca gcatgcacag cgacatgccg
      421 tatttcgaca gcgaatacgc caggtggccg gcgaaccatt tcggccgcag gtcgagcggc
      481 ggcgacaggg tgaggatgtg cgggttcgca gacttctgca gatagggcag gcaggcacgc
      541 gaggtgagga aggtgccgcg ggtgttgatc tgctggatga gatcgtagcg gcgcatgtcg
      601 atctgctgga tcgaacccag attgatggcg gaggcgttgt tgatgcagat gtcgatgccg
      661 ccgaagtgct gcgcacactg gcgagccgct gcgaggactg ccgcctcatc gcgtacgtcc
      721 acggcgagcg gcagcgcatg accacccgcc tgctcgatct cctgcgccgc cgtgtagatg
      781 gtgcctgcga gcttgggatg tggctcggcg gtcttggcgg cgatggcgat gttggcacca
      841 tcgcgcgccg cgcgcagcgc gatggccaac ccgatgccgc ggctcgcacc ggttatgaaa
      901 agcgtctgac cggatagact cactgcgccc tcccccagcg aaacgcgagt gtaccgcgcc
      961 gggctgcctc atccgcctgg cattgccgcc ttgagctcca cacggttgcc gtccgggtcc
     1021 tcgatataga aggagcggcc ctcgccctgc gcgccgtagc gtgagccggg ttcgcgcacc
     1081 agcacaccac gcgccacgag ccaggcgatg agcgcggact cctcgaaggg cgcgatggtc
     1141 aggcagaaat gctcgaggtt gcggcccgcg cccggtggcg cgccggcacc gagcgggcca
     1201 tcgagcgtga cgagatcgat gagactgcgt cccgcacgca gttgcgtgag cccgagctcc
     1261 ggctgctccc gctcgacctt acagccgaga acgtcgcagt aaaaggcgag cgagcgctgc
     1321 agttgctcca cccgcagcac gatatggtcg attccctgga ctgcgaacgg atgagctgcc
     1381 atcgatccct cccgacttgc gcccccgcga ggcgcaggta tagcgtgcca tgggcaggag
     1441 gcacaccggg agagcgcgat gcgctgggac gatctgcgcg agagcaccaa cgtcgaggat
     1501 gtacgcgcaa gcacgggcgg gggcgcgggt ctaaggctcg gcgtgggtgg cacgctgctc
     1561 gccctcgccg cctcttattt cctcgggatc gatccgcggc tgctcctcgg tttgatgagc
     1621 gcggtgccga cgcagcaatc ggctcccgcg gcgcactacg gcacgccgca ggatgagcag
     1681 ggacgcttca tcgccgccgt gctcggggag accgaggaca cttggagcgc gatcttccag
     1741 gagagcggcc gcgagtacat cccacccaaa ctcgtgctct atcgcgacag gatgccgacc
     1801 gcatgcggta ccggcagggc ggcggctggg cctttctact gcccgctcga ccgcaaggtc
     1861 tacctcgata tgagcttctt ccagcagctc tcggatgagt ttcaggctcc cggccaattt
     1921 gcccaagcgt acgtgctcgc gcacgaggtg ggtcatcacg tgcagaacct gctcggcata
     1981 gcggagcagg tccgctccgc acaggagcgc acctcgcagg cgcaggccaa tcagctctcg
     2041 gtgaagctcg agttgcaggc cgactgcttt gccggcgtgt gggcgcgcca cgccgatgaa
     2101 accaagcaaa tcctcgagca gggtgatgtg gaatctgcgc tgcgcgcggc gagcgcggtc
     2161 ggcgatgaca cgctgcagag acgcgcacag ggctacgtgg tgccggaatc cttcacccac
     2221 ggtacctcgg ccgagcgggt gagctggttc aagcggggca tggccgcagg ctcgatcgac
     2281 agctgcaaca ccttctcgac gccgacctag ccgacccctc cataccgcgc aggcgcgcgg
     2341 cggcgcacgg aggcgcgcag tcaacagagg ttttctcaac agttaaccaa acggtgaact
     2401 attcccgaaa acaccccaca agctgcgtcg ctcgggcgac gcattcgagt tgtttcaccc
     2461 gctcttgccg gaatagtgcg cgtcgagata gtcgaggatc tgcttcgctt cctcgtcggt
     2521 gatcggagca ccgaaccggt ccttcatctt ctggatggtt ttctgccagg cagcgcgatt
     2581 catcgccgga gcattgctcg ggatgtattc cacactgtgg cagatgatgc accggccgac
     2641 cgtgaggtcc cggcccggcc cttcgcgcag ctcggtcacc tcggccgccg cggcgggggc
     2701 gcccgacacg tgaccgacgg cgctggcaag gagtcccgcc gagagccacg cgagagaatg
     2761 gctgcggcgc atcaactcac ctccacgtgc gccgtctgca cgatgttgtc gtggtagcct
     2821 gcagggttgg gcgtgagctt ctccggctgt gttgagccat tgcggcttcg ggcgcgtacg
     2881 gcaatctcga tcggaccgct gcgcgaggta tcgagcggca gctggaactc gcgccaggcg
     2941 aaacgtccga ggtcgcggcc gagcttggcc tcgcgccagc tctggcgacc atcgacggac
     3001 acctccaccg ccgcgatgcc cgcacccgca tcccaggcct tgccgccgac atgcagcggc
     3061 tccccgcgcc gcacccgtgt cgagggggcc gggctcatga tgagcgagtt gatgaccatc
     3121 tcggtgatcg gcgtgttctc gggcgtttcc tggctgacga accgcgccgt cgcgaatgcg
     3181 ccggtcggga tacggtaggc cgacttcatc cagaaaccat cgaaggccgc cgcttgaacg
     3241 cggatctcgg tgagatgttt catccagtag gtgcccgtcc atcccggcac gacgagccgc
     3301 gcgggagcgc cattccagtg gggcagtggc tggccgttca tctcgaaggc gatgagcgtg
     3361 ctctcatcga gcgcacgatc gatcggcagg ctcttcacga aatccggcgt ccccggcagt
     3421 accgggccat ccgcaccgtt gaagaccacc tcgagcgcat cggcagccac gccggccttg
     3481 cgcagcacat cgcgcagccg caccccggtc cagagtgcat tcccgatcgc gccattgccc
     3541 cactgcacac cggccacccg tggagaaaag agtcctcgcc ggttgcccga gcactgattc
     3601 accgccggca cgctgacttt ctcgaacccg cgctgcagct cgcgca
//
LOCUS       DATDMP010000296         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_46565, whole genome shotgun sequence.
ACCESSION   DATDMP010000296 DATDMP010000000
VERSION     DATDMP010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46565"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /gene="rplS"
                     /locus_tag="VI195_05130"
     CDS             <1..197
                     /gene="rplS"
                     /locus_tag="VI195_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY6823800.1"
                     /translation="SNRGLNSSFTVRKISHGEGVERVFQTYSPAISDITVKRRGSVRR
                     AKLYYLRDLSGKAARIEEKI"
     gene            242..2083
                     /gene="sppA"
                     /locus_tag="VI195_05135"
     CDS             242..2083
                     /gene="sppA"
                     /locus_tag="VI195_05135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY6823801.1"
                     /translation="MMTLRSFFSGLWRGLDGLRKALNLILLLVIFGVLIAVLRGSVPR
                     IPSKAALLLVPEGELVEQLSGDPVERALQETRGETHRETLLWDLTDAIHAAASDPRVP
                     AIALDLEKFEGGTQPTLEELAAALREFRASGKKVIAYGAELTQERYYLASQADEVLLD
                     PIGFVLIDGYDRYRMYLKDALDKLGVEINVFRVGAFKSAVETFTRTNMSPEDREESRS
                     YLSALWSSYQGSVTRARKLQPDALAQYVGTLSKTVPAAGGDAARVALQAGLVSALKTR
                     LEFEQRLVALVGKDDYNGSFKAISLDDYVRYAHAQKKLKADGKPRVGVIVAEGEILDG
                     EQPPGTIGGESLARLIRQARTDKDMKALVLRVDSPGGSVLASEQIYRELVALRAAGKP
                     LVVSMSGYAASGGYYISAPADEIWASPATITGSIGIFAIIPTVDRTLGKVGVTVDGVG
                     TTPLSGQLRLDRPLGEEARTLLQSTVSRGYDEFLERVSSGRKKSRDEVDKVAQGRVWS
                     GSDAHRLGLIDQLGSFNDATRAAARRAKVTDYAVEFIEPELTWAQALAMQLRSHLAAL
                     LLRVSPGGAALAELAQRLDPVTREAQRLARFSAPGRLYAYCFCELSD"
     gene            complement(2123..>2332)
                     /gene="tdh"
                     /locus_tag="VI195_05140"
     CDS             complement(2123..>2332)
                     /gene="tdh"
                     /locus_tag="VI195_05140"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847610.1"
                     /note="converts threonine and NAD to
                     1,2-amino-3-oxobutanoate and NADH; functions in threonine
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY6823802.1"
                     /translation="VIFKGLTLKGIYGREMFETWYKMASLLQSGLKLEPIITHHFPVQ
                     EFQRGFETMGSGKSGKVILDWSTLQ"
BASE COUNT          382 a          790 c          799 g          361 t
ORIGIN      
        1 gcagcaaccg cggactgaac tcgtcgttca cggtgcgcaa gatctcgcac ggcgaaggcg
       61 tggaacgcgt gttccagacc tacagcccgg ccatcagcga catcaccgta aagcgccgcg
      121 gcagcgtgcg ccgcgccaag ctctattacc tgcgcgatct ctcgggcaag gccgcgagaa
      181 tcgaagagaa gatctgaccg cgcttgcggg cacctgggcg ccgccggcgg cgcaggagta
      241 gatgatgacg ctccgctcgt tcttttcggg cctgtggcgc ggcctcgatg ggctgcgcaa
      301 ggcgctcaac ctgatcctgc tgctggtgat attcggcgtg ctgatcgcgg tgctgcgcgg
      361 cagcgttccg cgcatcccct cgaaggcagc gctgctgctc gtgcccgaag gcgagctggt
      421 cgagcaactc tccggcgatc cggtcgagcg cgccctgcag gagacccgcg gcgagaccca
      481 ccgcgagacg ctgctgtggg atctcaccga cgccatccat gccgcggcga gcgacccccg
      541 cgttccggcg attgcactgg atctggagaa gttcgaaggg ggcacgcagc cgacgctcga
      601 ggagctcgcc gcggcactgc gtgagttccg cgccagcggc aagaaggtga tcgcctacgg
      661 agccgagctc actcaggagc gctactacct cgcctcgcag gccgatgagg tgctgctcga
      721 tccgatcggc ttcgtgctga tcgacggcta cgaccgctac cgcatgtatc tcaaggatgc
      781 gctggacaag ctcggcgtgg agatcaacgt gttccgcgtc ggcgccttca agagcgcggt
      841 ggagaccttc acccgcacca acatgtcgcc ggaggaccgc gaggagagcc gtagctacct
      901 cagcgcgctg tggagcagct accaggggtc ggtgacgcgc gcccgcaagc tccagcccga
      961 cgcgctcgcc cagtacgtcg gcacactctc gaagaccgtg ccggccgcgg gcggcgatgc
     1021 cgcgcgcgtc gcgctgcagg cggggctggt gagcgcgttg aagacgcggc tcgagttcga
     1081 gcagcgcctg gtggcgctcg tcggcaagga cgactacaac ggctccttca aggcgatctc
     1141 gctcgatgac tacgtgcgct acgcgcatgc gcagaaaaag ctcaaggccg acggcaagcc
     1201 gcgcgtcggc gtgatcgtgg cggaggggga gatcctcgat ggcgagcagc ccccgggcac
     1261 catcggcggt gagagcctcg cgcggctcat ccgccaggcg cgtaccgaca aggacatgaa
     1321 ggcgctggtg ctgcgagtcg acagcccggg aggcagcgtt ctcgcctccg agcagattta
     1381 ccgcgagctg gtcgcgctgc gcgctgccgg gaagccgctg gtggtgtcga tgagcggcta
     1441 cgcagcctcc ggtggctact acatctcggc gccggccgat gagatctggg cgagcccggc
     1501 gaccatcacc ggctccatcg gcatcttcgc catcatcccc accgtcgacc ggacactcgg
     1561 caaggtgggc gtcactgtcg atggggtcgg caccacacca ctctccggcc aactgcgcct
     1621 cgaccggccg ctcggagagg aggcacgcac gctgctgcag tccaccgtct cgcgtggcta
     1681 cgatgagttc ctcgagcgcg tctcgagcgg acgcaagaag tcacgtgacg aggtggacaa
     1741 ggtggcccag ggccgcgtgt ggtcggggag cgacgctcat cgcctcggcc tcatcgacca
     1801 gctgggttcc ttcaacgatg cgacgcgcgc cgcggcccgg cgcgccaagg tgaccgacta
     1861 cgcggtggaa ttcatcgagc ccgagctcac ctgggcgcag gcgctcgcca tgcagctgcg
     1921 ctcgcacctg gccgcgctgc tgctgcgtgt gagcccggga ggagcggcgc tcgccgagct
     1981 ggcgcagcgg ctcgacccgg tgacgcgcga ggcgcagcgc ctcgcacgct tcagcgcacc
     2041 cgggcgtctg tacgcgtact gcttctgtga gttgagcgac tgagcggcgc gggacggggc
     2101 cgcgggccgc gcggcctcgc ggtcactgca gcgtcgacca gtcgaggatg accttgcccg
     2161 acttccccga gcccatggtc tcgaagccgc gctggaactc ctgcaccggg aagtgatgcg
     2221 tgatgatcgg ctcgagcttc agcccgctct gcagcaggct cgccattttg taccacgtct
     2281 cgaacatctc ccggccatag atgcccttca gcgtgagtcc cttgaagatg ac
//
LOCUS       DATDMP010000297         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1046240, whole genome shotgun sequence.
ACCESSION   DATDMP010000297 DATDMP010000000
VERSION     DATDMP010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046240"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VI195_05145"
     CDS             complement(<1..477)
                     /locus_tag="VI195_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823803.1"
                     /translation="MKRRSLFYKYVIPIIGIVVVALAASGGISIYFSYQETRNALIAL
                     QREKATAAAARIENFVRGIEHQIGWTSLPQIAEGANPLELRRFDYVKLQKQVPAITES
                     IFIAPNGCMQVQESRLAMSRIGDCLERLDKDPRFTAARGGDTYFGPVYFRKETEPYM"
     gene            complement(474..1349)
                     /locus_tag="VI195_05150"
     CDS             complement(474..1349)
                     /locus_tag="VI195_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6823804.1"
                     /translation="MGTSERAQWARLLFAASMREAGFEEGRNLAIDWRYADGDVSRLP
                     ALAAELARLNVDLIMASFNDAIAAAKKATGTIPIVMFNSANPVEQGFVQSLARPGGNL
                     TGTAWSAPEVAAKILQVLHEAAPRARRVALIGNTSMPGVHDYGISAAKGAGAMGIETR
                     VFPLLRPEEMPRVLEQVAAYKAQALDIGIDTLLASRLEDIVEFANKHKLPAIANVSLF
                     TDVGGMLCYGPDIKELADRTAGYVTRILRGAKPADLPVELPSKFELVVNQKAARLIGH
                     RIPQSLLARADRVIE"
     gene            complement(1500..>2251)
                     /locus_tag="VI195_05155"
     CDS             complement(1500..>2251)
                     /locus_tag="VI195_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6823805.1"
                     /translation="RKLVLVKGPIQLQVAIDRRPAVVYLSHTDYRTGCRHDLAAVTRR
                     AHARGALAVWDLSHSAGAVETALARGDADFAVGCGYKYLCGGPGAPGYLYVAPRRQSR
                     VRPALQGWFGHAAPMAFEPRFRAAPGIQRHAVGTPAVAGNVLLEAALDVFAKFRPADL
                     FARHAELSQLLIQLAPKELALASPVDPRRRGGFVAFRFRNAKGLAQELERRKVVASAR
                     PPDILRFGLSPLYHTESDVRALARRLHAILG"
BASE COUNT          351 a          773 c          772 g          355 t
ORIGIN      
        1 catgtagggc tcggtctcct tgcggaagta caccggcccg aaataggtgt cgccgccgcg
       61 cgccgccgtg aagcgcgggt ccttgtcgag gcgctccagg cagtcgccga tgcggctcat
      121 cgcgaggcgc gattcctgca cctgcatgca gccgtttggc gcgatgaaga tcgattcggt
      181 gatcgccggc acctgcttct gcagcttcac gtagtcgaag cgccgcagct ccaggggatt
      241 cgcgccctcg gcgatctgcg gcagcgaggt ccagccgatc tggtgctcga tgccgcgcac
      301 gaagttctcg atgcgcgcgg cggccgcggt ggccttctcg cgctgcagcg cgatcagcgc
      361 gttgcgcgtc tcctggtagg aaaagtagat gctgatgccg ccgctggccg cgagcgcgac
      421 caccacgatg ccgatgatcg ggatgacgta cttgtagaag aggctgcgcc gcttcactcg
      481 atgacccggt cggcgcgcgc caggagcgac tgcgggatcc tgtggccgat cagcctggcg
      541 gccttctggt tgaccaccag ctcgaacttg ctcggcagct cgaccggcag gtcggcgggc
      601 ttggcgccgc gcaggatgcg cgtcacgtag cctgcggtgc ggtcggcgag ctccttgatg
      661 tccgggccgt agcacagcat gccgccgacg tcggtgaaca gtgagacgtt ggcgatcgcg
      721 ggcagcttgt gcttgttcgc gaactcgacg atgtcctcaa gcctgctcgc cagcagcgtg
      781 tcgatcccga tgtcgagggc ttgcgccttg tacgctgcga cctgctcgag caccctgggc
      841 atctcctcgg gacgcaggag ggggaagaca cgcgtctcga tgcccatcgc gcccgcgccc
      901 ttcgccgccg agatgccgta atcgtggacc ccgggcatcg acgtgttgcc gataagcgcc
      961 acgcgcctgg cgcgcggcgc ggcttcgtgc aggacctgca gaatcttggc tgcgacttcc
     1021 ggcgcgctcc aggcggtgcc ggtgaggttg ccgccggggc gtgccaggct ctggacgaag
     1081 ccctgctcca cgggattggc cgagttgaac atcacgatcg gaatcgtgcc ggtggctttc
     1141 ttggccgccg cgatcgcgtc gttgaacgat gccatgatca ggtcgacatt gaggcgcgcc
     1201 agttccgcgg ccagcgcagg cagccggctc acatcgccat cggcgtaccg ccaatcgatc
     1261 gccaggttcc gcccctcctc gaagccggcc tcccgcatcg aggcggcgaa cagcagcctg
     1321 gcccattgcg cgcgctcgga agtgcccacg ctgagcgaca gcacgccgat gcggcgcagc
     1381 ttgtccggct gctgtgcgag cgccgagagc ggcgccgcgc ccagtgccgt cagtgcaaga
     1441 atcgcttcac gcctgtccat ccgccctccc ctgagccgca ggcggaaatc tacacgcctt
     1501 cagccgagga tggcgtgcag tcgcctggcg agcgcccgga catcggattc cgtgtgatac
     1561 agggggctca ggccgaagcg caggatgtcg ggcggccgcg cggaggcgac gaccttccgc
     1621 ctttcgagct cttgcgccag cccttttgca ttccggaaac ggaaggcgac gaaacctccg
     1681 cgtcggcgag gatctaccgg gctcgccaac gccagttcct tcggcgcaag ctggatgagc
     1741 agctgcgaga gctcggcatg ccgggcaaac agatccgcgg gtctgaactt cgcgaaaaca
     1801 tccagcgcgg cctccaagag cacattgccc gcgaccgcgg gcgtgcctac ggcgtggcgc
     1861 tggatgcccg gcgcggcgcg aaaacgcggc tcgaaggcca tcggcgcggc atggccgaac
     1921 cagccctgca gcgcgggccg cacgcgactc tgccggcgcg gcgcgacgta gaggtaaccc
     1981 ggcgccccgg gcccgccgca gaggtacttg tagccgcagc cgaccgcgaa atccgcatcg
     2041 ccgcgcgcaa gcgccgtctc caccgcgccc gccgagtgcg aaaggtccca gaccgcgagc
     2101 gcgccgcgcg cgtgcgcccg gcgcgtgacc gcggcgagat cgtgccggca gccggtgcgg
     2161 taatccgtat ggctgagata gacgaccgcc ggacgccggt cgatcgccac ctgcagctgg
     2221 attgggcctt tcacgagaac aagcttgcgc c
//
LOCUS       DATDMP010000298         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_713773, whole genome shotgun sequence.
ACCESSION   DATDMP010000298 DATDMP010000000
VERSION     DATDMP010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713773"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VI195_05160"
     CDS             <1..1107
                     /locus_tag="VI195_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY6823806.1"
                     /translation="AIPFFIFAADLMARGKMAKQLADLFSALFGRYRGGVGHSTVAAV
                     MVFGAAAGSAPATVAAMGRIAFPELVRSGFSPRFSLGLIASSAECALLIPPSITFVVY
                     SWLTGTSIAGLFAAGLVVGIVLGVAFMGLVAWEAHRRGILGGARTEASARWRALREAG
                     WALGMQVIILVGIFAGFFTATEAAAVSVAYAVLVEVLVLRSLTWRDVFAIAQESAVTT
                     SVVFVLLAMGSLLAYFITLGEVQQTLLQWATEWHIGWVGFLMVVNVLFFIAGMFIDAN
                     SIMLIFLPTLFPIASSLGIDPVHFGMVVTLNICIGMITPPFGLDLFVASSSLKQPVED
                     VVAGVWPFIWVNLAVLMVISYVPSISTALPKLML"
     gene            1104..1904
                     /locus_tag="VI195_05165"
     CDS             1104..1904
                     /locus_tag="VI195_05165"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY6823807.1"
                     /translation="MKVATYACEIVEHAWVNKRYKYLRLRCPEPLARRTLAGQFFQLA
                     CPVTETEHPFLRRPMSVYGIGPQDDRIDFLYHVKGVGTRALEGLRQGERLAIIGPLGR
                     GFSFSPAWRRILLVARGVGLATMAPLVPLAGRAGVAMTAVLSARTPGDLMRAEFLRGI
                     EAEVHSVFDSDGSSSVENVDALLGRLLAAQRPDMVYTCGSNRLLGVLQKHDVVGEVAL
                     EQQMACAMGVCLSCVRLFHEGDAKVFRRVCCEGPVFPIAAVAGEVQFG"
     gene            1897..>2202
                     /locus_tag="VI195_05170"
     CDS             1897..>2202
                     /locus_tag="VI195_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HEY6823808.1"
                     /translation="MVELGVRIGTLELKNPVMPASGCFAHEYADAMDLARLGAVVTKS
                     VTPKRRIGNPVPRVAETEAGMLNSIGIPSNGLLYYVDSILPHYAGVAAPLVASVSADT
                     "
BASE COUNT          288 a          725 c          742 g          447 t
ORIGIN      
        1 gcgatcccgt tcttcatctt tgccgccgac ctgatggcga ggggaaagat ggcgaagcag
       61 ctcgccgatc ttttcagcgc gcttttcggc cgctaccggg gcggcgtggg ccattccacg
      121 gtcgcggcgg tcatggtgtt cggcgccgcc gccggctccg cgcccgccac cgtcgcggcg
      181 atgggacgca tcgcgttccc ggagcttgtg cgctccgggt tttcgccacg cttcagcctg
      241 gggctcatcg cgtcctcggc ggagtgcgcg cttctcatcc cgccatccat cacgttcgtg
      301 gtgtacagct ggctcacggg cacctccatt gccgggcttt tcgccgccgg gcttgtggtc
      361 ggcatcgtgc tcggcgtggc tttcatgggc ctcgttgcct gggaggcgca ccgtcgcggg
      421 atccttggcg gcgcgcgcac tgaggccagc gcgcgctggc gcgccttgcg cgaggccgga
      481 tgggcgctgg gcatgcaggt gatcattctc gtcggcatct tcgccggctt cttcacggcc
      541 accgaggcgg cggccgtatc ggtcgcctat gccgtgctcg tcgaggtgct cgtgctgcgc
      601 tcgctgacat ggcgcgacgt gttcgccatc gcgcaggaga gcgcggtgac cacttcggtg
      661 gtgttcgtgc tgctcgccat gggctcgctt ctcgcctact tcatcacgct cggcgaagtc
      721 cagcagacgc tcctgcaatg ggccaccgag tggcacatcg gctgggtggg ctttctcatg
      781 gtggtgaacg tgctcttctt cattgccggc atgttcatcg acgccaactc catcatgctc
      841 atcttcctgc cgacgctttt cccgatcgct tcgtccctcg gcatcgaccc ggtgcacttc
      901 gggatggtcg tcaccctgaa catctgcatt ggcatgatca cgccgccctt cgggctggat
      961 cttttcgtcg cctcgagctc cctcaagcag ccggtggagg atgtcgtggc gggggtctgg
     1021 cccttcatct gggtcaacct ggcggtgctg atggtcatct cctacgtgcc gtccatctcg
     1081 accgcgctgc cgaagctgat gctatgaagg tcgccacgta cgcgtgcgag atcgtggagc
     1141 acgcctgggt gaacaagcgc tacaagtacc tgcgcttgcg ctgcccggag ccgctcgcgc
     1201 ggcgtacgct cgccggccag tttttccagc tcgcctgccc cgtgacggag accgagcatc
     1261 cattcctgcg ccggccgatg agcgtgtacg gcatcgggcc gcaggatgat cggatcgatt
     1321 tcctgtacca cgtcaagggc gtcggcaccc gggcgctgga aggcctgcgg cagggcgagc
     1381 ggctcgccat catcggcccg cttgggcgag gcttcagctt ctcgccggcg tggcgccgga
     1441 ttctcctcgt ggcgcgcggc gtaggtcttg ccacgatggc gcctctcgtc ccgctggctg
     1501 gccgcgccgg cgtcgccatg accgccgtgc tctcggctcg cacaccgggc gacctgatgc
     1561 gggccgaatt tctgcgaggc atagaagcgg aggtgcacag cgtcttcgac agtgacggca
     1621 gctcctcggt ggaaaatgtg gacgcgcttc ttggccggct gctggccgcc cagcggcccg
     1681 acatggtgta cacctgcggc tcgaaccgtc tgctaggcgt actgcagaag catgacgtcg
     1741 tgggcgaagt cgcgctcgag cagcaaatgg cttgcgccat gggggtgtgc ctctcgtgcg
     1801 tgcgcttgtt ccacgagggc gacgccaagg tcttccggcg cgtgtgctgc gaaggaccgg
     1861 tgttcccaat agcggcggtc gctggcgagg tgcagtttgg ttgagctcgg tgtacgcatc
     1921 gggacgctcg agctgaagaa tccggtgatg ccggcttcgg gctgctttgc gcacgaatac
     1981 gccgatgcaa tggacctcgc gcgcctcggc gcggtggtca ccaaaagcgt cacgccgaag
     2041 cggcgcatcg gcaatccggt gccgcgcgtc gcggagaccg aggcggggat gctgaactcc
     2101 atcggcatcc cttcgaacgg cctgctctac tacgtggaca gcatcctgcc gcattatgcc
     2161 ggggtggcgg cgccgctcgt ggcgagcgtt tcagccgaca cc
//
LOCUS       DATDMP010000299         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_713797, whole genome shotgun sequence.
ACCESSION   DATDMP010000299 DATDMP010000000
VERSION     DATDMP010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713797"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VI195_05175"
     CDS             <1..868
                     /locus_tag="VI195_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823809.1"
                     /translation="GNAMVKVRIAPNNPVFDLPVIVGIEEGLFKQAGLDVSFSATYAD
                     RERDTAEKPIFERLKEEMFECGSADSYNVCEWASIDRLERGTRGGKLAALRAAVAAQA
                     IVTFDDKLQTPRDMADVPVVVQELTGSHYTTIQMLESAIGREHVKIQQGGLPQRRWEG
                     LKNGSYRAVTVMEPFISLALKEGAHIIAASFYRGGEVVAAELTPEQRKSYYDAENKAV
                     DLINADFYKYAHHVAAHAKGALKPNELLRAFVHYKHVDYYDPELFNRAYDWMRARGMT
                     EGQSQHAALVVG"
     gene            complement(865..1476)
                     /locus_tag="VI195_05180"
     CDS             complement(865..1476)
                     /locus_tag="VI195_05180"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6823810.1"
                     /translation="MRATRSLLFLSVLLGLFLQRAVADELPPDVLVKTVTLEVVDIIA
                     KDREIKSSKAKLVSLVNAKVLPHFDFVGMTRLAVGPAWNKATPEQRNHLSEQFKTLLV
                     RTYSSALAAYSDQKFEFRPLRMKPGESDVTVTVRVLQHGAQPVAIEYSMEKTSTGWKV
                     YDMLVGGVSLVANYRTEFANKIQRAGVDGLIRELQAKNRSQEI"
     gene            complement(1541..2566)
                     /locus_tag="VI195_05185"
     CDS             complement(1541..2566)
                     /locus_tag="VI195_05185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY6823811.1"
                     /translation="MHFGIFDWVEASDTRSAREVYEHKLELAAAADRAGLHSYLLAEH
                     QGTPLSIDGSPSVLLSAMFQRTRKLRAGGLTFCLPWYDPYRFYNEVCMLDHLSGGRLE
                     LGVGRGVSPIESKIFGMQDIEQSRDRYRETLEVFFAACASDTLNFSGKHFRYQDAELH
                     LKPVQKPYPPLWFPSSNKESIDFTARHGYHTAFLGRLADCKPHFERYRETWRQHRNDP
                     GRHNAHVAEPYLAKTQHLVIAEDEREAERRGLEAYWKWAGHIHHLTRKLGRPDVHKND
                     PYAEDSAHRLITGSPKTAAAKLKEMLEVTSANYLLCVFSFGDLAPEHAMRSLQLFARE
                     VMPVLKV"
BASE COUNT          490 a          842 c          893 g          468 t
ORIGIN      
        1 gggaaacgcc atggtcaaag tgaggattgc tcccaacaac ccggtcttcg acctgccggt
       61 gatcgtcggc atcgaggaag gactcttcaa gcaggcgggc ttggacgtca gcttctccgc
      121 cacctatgcc gaccgcgagc gcgataccgc ggaaaagccg atcttcgagc gcctgaagga
      181 agagatgttc gaatgcggct cggccgacag ctacaacgtc tgcgaatggg cgagcatcga
      241 tcgcctggag cgcggcacgc gcggcggcaa gctcgccgcg ctgcgcgcag cggtcgcggc
      301 gcaggcgatc gtcaccttcg atgacaagct ccagaccccg cgcgacatgg cggacgtccc
      361 ggtggtggtg caggagctga ccggctcgca ctacaccacg atccagatgc tcgaaagcgc
      421 catcgggcgc gagcacgtga agatccagca gggcgggctg ccgcagcggc gctgggaagg
      481 cctgaagaac ggcagctatc gcgccgtcac cgtcatggag cctttcatca gcctcgcgct
      541 aaaggaaggc gcgcacatca tcgccgcctc gttctaccgc ggcggcgaag tcgtcgccgc
      601 cgagctcacc ccggagcagc gcaagagcta ctacgacgcc gagaacaagg cggtggacct
      661 catcaacgcc gacttctaca agtacgcgca ccacgtggcg gcgcatgcga agggcgcgct
      721 gaagccgaac gagctgctgc gcgccttcgt gcattacaag cacgtcgatt actacgatcc
      781 ggagctcttc aaccgcgcct acgactggat gagggcccgc ggcatgaccg aagggcagag
      841 ccagcacgcg gcgctggtcg tcggctagat ttcctgcgag cggttcttcg cctgaagctc
      901 gcggatgagg ccgtcgacgc cggcccgctg gatcttgttg gcgaactcgg tgcggtagtt
      961 ggcgacgagg cttacgccgc cgacgagcat gtcgtacacc ttccagccgg tcgaagtctt
     1021 ctccatgctg tactcgatgg ccacgggctg cgcgccgtgc tgtagcacgc gcaccgtgac
     1081 cgtgacatca ctctcgccgg gcttcatgcg cagcggccgg aattcgaatt tctggtcgct
     1141 gtaggcagcg agcgcgctgg agtaggtgcg cacgaggagt gtcttgaact gctcggagag
     1201 gtggttcctt tgctccggcg tggccttgtt ccatgctggc ccgaccgcaa ggcgggtcat
     1261 accgacgaag tcgaaatgcg gcaggacctt tgcattgacc aggctaacga gcttcgcctt
     1321 gctcgacttg atttccctgt ccttggcgat gatgtcgacc acctccagcg tcacggtctt
     1381 tacgaggaca tccggaggaa gttcgtcggc gacggcgcgc tgaaggaaga gaccgagaag
     1441 gaccgataag aacagtagtg aacgggtggc tcgcatggcg agaattcaac catccctcgg
     1501 cccggccgca aaagacctcc gcaaccggcc aaacacattt ttacaccttg agtactggca
     1561 tcacctcgcg cgcgaaaagc tggagcgagc gcatcgcgtg ctcgggcgcg aggtcgccga
     1621 aggagaaaac gcagagcagg tagttggcgc tcgttacctc cagcatttcc ttcagcttcg
     1681 cggcggcggt tttcggcgag ccggtgatga ggcggtgcgc ggagtcctcc gcgtagggat
     1741 cgttcttgtg cacgtccggc cgcccgagct ttcgcgtcag gtggtggatg tggccggccc
     1801 acttccagta ggcctcgaga ccccgtcgct cggcctcgcg ctcgtcctcc gcgatgacga
     1861 gatgctgggt cttcgcgagg tagggctccg cgacatgggc gttatgccgc cccgggtcgt
     1921 tgcggtgctg ccgccaggtc tcgcggtagc gctcgaagtg cggcttgcag tcggcgagcc
     1981 tgccgaggaa cgcggtgtga tagccgtggc gggcggtgaa gtctatggat tccttgttcg
     2041 agctcggaaa ccagagcggc gggtagggct tctgcaccgg cttgagatgc agctctgcat
     2101 cctggtatct gaagtgcttg ccggaaaaat tcagcgtatc cgaggcgcag gccgcaaaga
     2161 aaacctccag cgtttcccga taccggtcgc gcgactgctc gatgtcctgc atgccgaata
     2221 ttttcgactc gatgggcgac acgccgcggc cgacgccgag ctccaggcgc ccgcccgaca
     2281 ggtggtcgag catgcacact tcgttgtaga agcggtaggg gtcgtaccac ggcaggcaga
     2341 acgtcaggcc gcccgccctg agcttgcgcg tgcgctggaa cattgccgag agcaggacgc
     2401 tcggcgagcc gtcgatcgaa agcggcgtgc cctggtgctc ggccaggaga taggagtgaa
     2461 gtccggctcg gtcagccgcg gcggcgagct cgagcttgtg ctcgtagacc tcgcgcgcag
     2521 agcgcgtatc cgaggcttcc acccagtcaa agattccgaa gtgcatttca gctccgcgca
     2581 tttacacgcc gagatagcga tgccacaggc cgcggtcggc cgcgagctcg gcggagctgc
     2641 cctgccaggc gacccggccc cgctcgatga tcgtgtgccg atcggcgagc gcc
//
LOCUS       DATDMP010000300         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1269709, whole genome shotgun sequence.
ACCESSION   DATDMP010000300 DATDMP010000000
VERSION     DATDMP010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269709"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VI195_05190"
     CDS             complement(<1..711)
                     /locus_tag="VI195_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY6823812.1"
                     /translation="MSPLVLTSFTATSAIGRGLDATLASLRAQRSGLAPCHFETVALE
                     TCVGEVPGVDEVQLPRKLNEFDCRNNRLAWLAMAQDGFTAAVAQRVARYGAQRLGVFL
                     GTSTSGILETEIAYRHRDPQSGALPEGFRYRGVHNTYSLAAFTKRALDLTGPAVVISC
                     ACSSSAKVFASARRAIEAGLIDAAVVGGVDSLCLTTLYGFHALQLVARTPCRPFDAAR
                     DGISIGEAAAFALLERPQN"
     gene            complement(708..1442)
                     /locus_tag="VI195_05195"
     CDS             complement(708..1442)
                     /locus_tag="VI195_05195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6823813.1"
                     /translation="MSRQGTLAPLIDAASAYYRGAGRFAWHFARGKLATDPVFAAMLA
                     HGLLSGRARILDLGCGQGLLAAWLLAARGLHASDAVGAWPPGWPAPPLLHDYTGIEIN
                     PREVERARRACALDPGAQVRIIHGDIRDADYASADVVVILDVLHYLDQVAQEHVLRRV
                     RSALGPQGLLLLRVGDAAGGMRFTFSKAVDATVALARRRRWIRLECRPLRAWERLLSG
                     CGFRTHALPMSRGTPFVNVLLRAELA"
     gene            complement(1439..2239)
                     /locus_tag="VI195_05200"
     CDS             complement(1439..2239)
                     /locus_tag="VI195_05200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6823814.1"
                     /translation="MTILRPHWWPWTLSALIADHLLLAGSGLWPRSRLLGPNWTHLPP
                     VASGAGSAAPPAAVAITIDDGPDPEVTGRVLEVLDAHGAVATFFCIGERVERHRALAR
                     AIVARGHEIANHSYRHLMSFSLLGPRGVAREISRAQQAIGDTTGEIARFFRAPAGLRN
                     PFLDPALTRANLQLVSWTRRGFDTVYGSTGRVLGSLIRDLRDGDILLLHDGHAARTPS
                     GTPVILEVLPRLLRALTEAQLTPITIRAAFERAAAVPAPLSAGPGARP"
     gene            complement(2396..>2579)
                     /locus_tag="VI195_05205"
     CDS             complement(2396..>2579)
                     /locus_tag="VI195_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823815.1"
                     /translation="LGLGREDRFGRRQELLAAALSPANVDTVPGGHDWATWRTLWDRF
                     LEVRLAPEVRAPRSVT"
BASE COUNT          427 a          856 c          942 g          354 t
ORIGIN      
        1 attctgcggg cgctcgagga gcgcgaaggc cgcggcttcg ccgatggaga tgccatcgcg
       61 cgccgcatcg aaaggccggc acggcgtgcg cgccacgagc tgcagcgcat gaaagccgta
      121 gagcgtcgtg aggcaaaggg agtccacgcc gccgacgacc gcggcgtcaa tcagtccggc
      181 ctcgatggcg cggcgcgccg aggcgaacac cttggcgctc gaggagcagg cgcacgagat
      241 cacgaccgcg ggtcccgtga gatcgagcgc gcgcttcgtg aacgcggcga gcgagtaggt
      301 gttatggaca ccgcgatagc ggaacccctc cgggagcgcg ccgctctggg ggtcgcgatg
      361 gcggtaggca atctcggtct cgaggatgcc ggaggtgctg gtaccgagga acaccccgag
      421 tcgctgtgca ccgtagcgcg ccacgcgttg tgcgaccgcc gcggtgaacc catcctgcgc
      481 catcgccagc caggcgaggc gattgttgcg gcaatcgaac tcgttgagct ttcgcggcag
      541 ctgtacctca tcgactcccg gtacctcgcc cacgcaggtc tcgagagcca cggtctcgaa
      601 gtggcagggc gcgagtcccg agcgctgtgc gcgcagactc gcgagggtcg catcgagtcc
      661 gcgaccgatg gcgctcgttg cggtgaagct cgtgaggacg agtgggctca tgcgagttcg
      721 gcccgcagca gcacgttgac gaagggagtg ccacggctca tcggcagcgc atgcgtgcgg
      781 aaaccgcagc cgctcaagag acgctcccag gcgcgcagtg gccggcactc gagccgtatc
      841 cagcggcggc ggcgcgcgag tgccaccgtg gcgtcgacgg ccttgctgaa tgtgaagcgc
      901 ataccgcctg ccgcatcgcc tacgcgcagc agcagcagac cctgcgggcc aagagcgctg
      961 cgtacgcggc gcagtacgtg ctcctgtgcc acctggtcga ggtaatggag tacatcgagg
     1021 atcaccacga catcggcgct ggcgtagtcc gcatcgcgga tgtcaccgtg gatgatccgc
     1081 acctgcgcac ccgggtcgag cgcgcacgca cggcgtgcgc gctcgacctc ccgcgggttg
     1141 atttcgatgc cggtgtagtc atgcagcaag gggggcgcgg gccaccccgg cggccatgcg
     1201 cccactgcat cgctcgcatg caggccacgc gctgcgagca gccaggcggc caggagaccc
     1261 tggccgcaac caagatcgag gatgcgtgcg cggccactta gcagtccgtg cgcgagcatg
     1321 gcggcgaaca ccggatcggt cgccagcttg ccgcgcgcga agtgccaggc aaagcggccc
     1381 gctccgcggt agtaagcgct tgcggcgtcg atgagcggcg caagcgtccc ctggcggctc
     1441 acgggcgtgc gccggggccg gcggacagcg gagcgggtac cgcggcggct cgctcgaatg
     1501 ctgcccggat cgtgatcggt gtcagctgcg cctcggtgag cgcccgcaga aggcgcggca
     1561 acacctcgag gattacgggc gtccccgatg gggtgcgcgc ggcgtgaccg tcgtgcaaca
     1621 gcaggatgtc gccgtcccga agatcccgaa tgaggctgcc aaggacgcgg ccggtgctac
     1681 cgtagacggt gtcgaaacca cggcgtgtcc agctcaccag ctgcagattg gcccgcgtca
     1741 acgccggatc gaggaacgga ttacgcaatc ccgccggggc gcggaagaag cgggcgatct
     1801 ccccggtcgt gtcgccgatc gcctgctgtg cccgggagat ctcgcgtgcc acgccgcggg
     1861 gcccaagaag cgagaagctc atgagatgac ggtagctgtg gttggctatc tcgtggcctc
     1921 gcgcgacgat ggcgcgtgcg agcgcgcggt ggcgctcgac gcgctcgccg atgcagaaga
     1981 aggtcgcgac ggcgccatgc gcatcgagca cctcgagcac acggccggtg acttccgggt
     2041 caggcccgtc atcgatggtg atagccaccg cggcgggcgg ggcagcgctg cctgcgccgc
     2101 tcgcaaccgg tggaagatgt gtccaattgg ggccgagcag gcggctacgg ggccacagtc
     2161 cactcccggc gagcagaaga tgatcggcga tgagcgccga gagcgtccag ggccaccaat
     2221 gcggtcgcag gatcgtcaca ccggcggcac ccgcgtgcag ggccacggag gcccggatga
     2281 gcggggacgg ccgccaccgc cgcgcgcttg ggtgaggagg gggtatcccg tgcaccggtc
     2341 gatccatgta ggaaattctg ccgtgcgcgc gtcgatcccg cgagcctcgc gcgcttcagg
     2401 ttacgcttcg cggcgcgcgt acctccggcg caagcctcac ctccagaaag cggtcccaaa
     2461 gggtgcgcca ggttgcccag tcgtgcccgc cgggtacggt gtccacgttc gccggtgaga
     2521 gcgccgccgc cagcagctcc tggcggcggc cgaagcggtc ctcgcggccc aggccgaga
//
LOCUS       DATDMP010000301         2150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1380588, whole genome shotgun sequence.
ACCESSION   DATDMP010000301 DATDMP010000000
VERSION     DATDMP010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380588"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VI195_05210"
     CDS             <1..654
                     /locus_tag="VI195_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6823816.1"
                     /translation="RDWLPELNARGLIDAVDVFCDAIAFSVAQAARLFAAARSLGLPV
                     KMHAEQLSNLGGTLMAAAHGALSCDHLEYAGAEEAGALGRSGTVAVLLPVAFYCLGAG
                     RQPPLAALRSARAALAIASDCNPGSAPGASLLLAMSMATRLFGLTAEEALLGVTRHAA
                     QALGLGAGRGALSAGPAADFVVWNIGSPAELSYWIGFNPRRTVVRAAEVLPWPHMTP"
     gene            633..>2150
                     /gene="hutH"
                     /locus_tag="VI195_05215"
     CDS             633..>2150
                     /gene="hutH"
                     /locus_tag="VI195_05215"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY6823817.1"
                     /translation="MAPHDPLKIGGRPLRLAELRRVYEHPLTVEIDPAALGAVRAAHT
                     ATARLAAAEQAAYGINTGFGLLAQTRIPTAQRTLLQRNIILSHSCGVGPPLEDAVVRL
                     TLVLKLASLLQGFSGVSEELVRFLERLVNAGLYPCVPSQGSVGASGDLAPLAHLSLSV
                     LGLGELRRHGEVLPAAEALAQEGLAPLALGPKEGLALINGTQVSTALALAGLFELETA
                     FGAAVVSGALSVDALAGSDVPFDPRIHSLREHGGQARVAQVYRALLAGSEIRESHRSC
                     PRVQDPYSLRCQPQVMGACLDLMTQQAATLATEANAVTDNPLIFAESGEVLSGGNFHA
                     EPVAFAADILALACTEVASIAERRIALLVDPHMSGLPPFLAADSGVNSGFMLAQVTAA
                     ALVAENRMLSHPASVDSIPTSANQEDHVSMATHGARRLLTMTRNTLTVAGIEFLTAAQ
                     GVDFRAPLKTSAALAGALGALRLKVPFARSDRLLALDVEEAVRVVRSREVQSLARE"
BASE COUNT          262 a          802 c          775 g          311 t
ORIGIN      
        1 cgcgactggc tgccggagct caacgcccgc gggctcatcg atgcggtgga cgtgttctgt
       61 gacgctatcg ccttcagcgt cgcgcaggcc gcgcggctgt tcgcggcggc ccgctccctc
      121 ggcctgccgg tcaagatgca tgccgagcag ctttccaacc tcggcggcac gctcatggcc
      181 gccgctcacg gtgcgctctc ctgcgatcac ctcgagtacg ccggcgccga ggaagcgggc
      241 gcgctcgggc gctccggcac ggtagcggtg ctgctgccgg tggctttcta ctgcctgggc
      301 gccggccggc agccgccgct cgccgcgctg cgctcggcgc gcgccgcgct cgcgatcgcc
      361 agcgactgca acccggggtc ggcgccgggc gcctcgctgc tgctcgcgat gagcatggcg
      421 acgcgcctct tcgggctcac ggccgaagag gcgctgctcg gcgtcacgcg ccacgccgca
      481 caggcgctcg ggctcggcgc cgggcgcggc gcactgagcg ccggccccgc cgccgacttc
      541 gtggtgtgga acatcggctc gcccgccgag ctgagctact ggatcggctt caacccgcgc
      601 cggaccgtgg tccgggcggc ggaggtgctg ccatggcccc acatgacccc ctgaagatcg
      661 gcggccgccc gctgcgcctc gcggagctgc ggcgggtgta cgagcatccg ctcaccgtgg
      721 agatcgaccc cgccgcgctc ggcgcggtac gcgcagccca taccgccacc gcgcggctcg
      781 cggccgccga gcaggccgcc tacgggatca acaccggctt cgggctgctc gcccagaccc
      841 gcatccccac cgcgcagcgc acgctgctgc agcggaacat catcctgtcg cacagctgcg
      901 gcgtgggtcc cccgctcgag gacgcggtgg tgcggctcac cctggtgctg aagctggcga
      961 gcctgctgca gggcttctcg ggcgtgagcg aggagctggt gcgcttcctc gagcggctgg
     1021 tgaacgccgg gctctacccg tgcgtgccgt cgcagggctc ggtcggtgcc tccggcgacc
     1081 ttgcgccgct tgcgcacctg tcgctgagtg tcctcgggct cggcgagctg cggcgccacg
     1141 gggaggtgct gccggcggcc gaagcgctcg cccaggaggg gctggcgccg ctcgcgctcg
     1201 gaccgaagga ggggctggcg ctcatcaacg gcacgcaggt ctccaccgcg ctcgcgctcg
     1261 cggggctgtt cgagctcgag accgcattcg gcgccgcggt ggtgagcggc gcgctctcgg
     1321 tcgacgcgct cgccgggagc gatgtgccct tcgacccgcg catccacagt ctgcgcgagc
     1381 acggcgggca ggcgcgcgtt gcgcaggtct accgcgcgct gctcgcgggc agcgagatcc
     1441 gcgagtcgca tcgcagctgt ccgcgggtgc aggatccgta ctcgctgcgc tgccagccgc
     1501 aggtgatggg cgcgtgcctc gacctgatga cgcagcaggc cgcgaccctc gccaccgaag
     1561 ccaacgccgt caccgacaac ccgctgatat tcgccgagtc gggcgaggtg ctctcgggcg
     1621 gcaacttcca cgccgagccg gtggccttcg ccgccgacat cctcgcgctg gcatgcaccg
     1681 aggtggcctc gatcgccgag cgccgcatcg cgctgctggt cgatccgcac atgagcggac
     1741 tgccgccgtt tctcgccgcc gacagcggcg tgaactccgg cttcatgctg gcgcaggtga
     1801 ccgccgcggc actggtggcg gagaaccgca tgctgagtca cccggcgagc gtcgacagca
     1861 tcccgacctc ggccaaccag gaggatcatg tcagcatggc gacccacggc gcgcggcggc
     1921 tgctcaccat gacgcgcaat acgctcacgg tggcgggcat cgagttcctg accgccgccc
     1981 agggcgtcga tttccgcgcc ccgctcaaga cctcggcggc gctggccgga gcgctcggag
     2041 cgctgcgcct caaggtgccg tttgcgcgca gcgaccggct gctcgcgctc gatgtcgagg
     2101 aggccgtgcg ggtggtgcgc tcgcgcgagg tgcaatcact cgcccgggag
//
LOCUS       DATDMP010000302         3408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_47018, whole genome shotgun sequence.
ACCESSION   DATDMP010000302 DATDMP010000000
VERSION     DATDMP010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47018"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /gene="trpS"
                     /locus_tag="VI195_05220"
     CDS             complement(<1..820)
                     /gene="trpS"
                     /locus_tag="VI195_05220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY6823818.1"
                     /translation="MFQDRVLSGMRPTGAMHLGHYHGALKNWVRLQNEYPCYFFAADW
                     HALTTHYEDPGVIEQTVWDMFVDWLCAGIDPTKSTLFIQSRVPQHAELTMLLSFFTPL
                     AWLERVPTYKDLVEKHAEHGRDVLTYGFLGYPLMQAADILIYRANKVPGGEDQVSHIE
                     LTREIARRFNHLFGREKGFEEKAEAAIKKMGPKKAKLYRSLRQAFQEQGDEKALEQAR
                     ELVEAVQNLTLGDKERLYGFIEGGGRMILSEPQALLTPTSKLLGLDGQKMSKSYD"
     gene            complement(829..1485)
                     /locus_tag="VI195_05225"
     CDS             complement(829..1485)
                     /locus_tag="VI195_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY6823819.1"
                     /translation="MDPTNVLIKILVYALPVIFAITLHEAAHGYVARHFGDSTAYMLG
                     RVSLNPVRHIDPIGTIVLPIVTLLLSGFMFGWAKPVPVNFQNLRHPKRDSLWVAAAGP
                     ASNLAQALIWAAVARVLSEVISPTGLAGGFWLAVAEAGVVVNVAFAILNLVPLLPLDG
                     GRMLASLLPGRLSYQYSRLEPYGMIILILLIVTPVLGRVLNPLVLGTLRAIYSLFGLQ
                     "
     gene            complement(1485..2123)
                     /locus_tag="VI195_05230"
     CDS             complement(1485..2123)
                     /locus_tag="VI195_05230"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004730625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY6823820.1"
                     /translation="MAQFFTVHPDNPQPRLIRAAVDIIRRGGVVAYPTDSCYALGCHI
                     GDKAAMERLRRIRDVDERHHFTLMCRSLSEVSLFAKVDNLQYRLIRANTPGRYTFILR
                     ATRDVPRRLLTPRHTIGVRIPAHPVPMAMLEHLDEPLLSTTLILPAHGVALNDAEEIR
                     QHLEHHVDLVIDSGACDGEVTTVVDLSGETPRLVREGAGDIRPFGFVKAALH"
     gene            complement(2188..3165)
                     /locus_tag="VI195_05235"
     CDS             complement(2188..3165)
                     /locus_tag="VI195_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY6823821.1"
                     /translation="MGEPRRADLRGEAAQQGGRGELALVAENDRLHTQFCYNFWCLPN
                     LSRLENFDLHNHSTRSDGLLSPTQLIELASRTGVDAIALTDHDTVEGLEEAQSAARAL
                     GVGFVPGVEISVSWGETTLHIVGLRVDPHSRVLRDGLDSVRAGRVGRARRIGEALTRM
                     GFANTFEGALELAGDERIVSRTHFARYLAESGAVKDTQAAFDKWLAKGRPAFVPHHWA
                     ALDDAVKWIIAAGGTAVVAHPGRYDLKPMMRDEMLQQFRAAGGRAIEVVTGSHRPEQY
                     AAWQRVALEHGFLASRGADYHGPGESPCEPGKLPALHASLTPVWSSWAH"
     gene            3070..>3408
                     /locus_tag="VI195_05240"
     CDS             3070..>3408
                     /locus_tag="VI195_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823822.1"
                     /translation="MQPIVFGDEGQLATATLLRRFAAEIRPAGLPHAAEAERWLRTLD
                     SLDRHYGGAGALPLEDAADVLAATLSALARLAQDAPPDLHGAVADITLGTAIWALRHD
                     IVLDPVEPVVN"
BASE COUNT          550 a         1129 c         1198 g          531 t
ORIGIN      
        1 tgtcgtagct cttcgacatc ttctggccgt cgaggcccag cagcttggac gtgggcgtga
       61 gcagcgcctg gggctccgag aggatcatgc ggccgccgcc ctcgatgaag ccgtagagcc
      121 gctccttgtc gccgagggtg aggttctgca ccgcttccac cagctcgcgc gcctgttcga
      181 gcgccttctc gtcgccctgc tcctggaagg cctggcgcag gctgcggtag agcttggcct
      241 tcttgggtcc catcttcttg atcgcggcct cggccttctc ctcgaagccc ttctcgcggc
      301 cgaagaggtg gttgaagcgc ctcgcgatct cgcgggtgag ctcgatatgg gagacctggt
      361 cctcgccccc ggggaccttg ttcgcccggt agatcaggat gtccgccgcc tgcatgagcg
      421 ggtatccgag gaagccgtag gtaagcacgt cgcggccgtg ctcggcgtgc ttttccacca
      481 ggtccttgta cgtcggcacg cgctcgagcc acgcgagcgg cgtgaagaac gacagcagca
      541 tcgtgagctc ggcgtgctgc ggcacgcgcg actggatgaa gagcgtggac ttggtggggt
      601 cgatgccggc gcagagccag tcgacgaaca tgtcccagac cgtctgctcg atcacgcccg
      661 gatcctcgta gtgcgtggtg agcgcgtgcc agtcggcggc gaagaaatag cagggatatt
      721 cgttctgcag gcgcacccag ttcttcagcg cgccgtggta gtggccgagg tgcatggcgc
      781 cggtggggcg catgccggag aggacgcggt cctggaacat cgtggtcgct attggaggcc
      841 gaaaagggag tagatggcgc gcagggtgcc cagcacaagc gggttcagca cgcggcccag
      901 cacgggtgtc acgatcagca ggatgaggat gatcatcccg tagggctcga ggcgcgagta
      961 ctggtacgag aggcggccgg gcagcaggct cgcgagcatg cggccgccgt cgagcggcag
     1021 cagcggcacg aggttgagga tggcgaaggc cacgttcacc acgacgccgg cctccgccac
     1081 ggcgagccag aagccgcccg cgagcccggt gggcgagatc acctccgaga gcacgcgtgc
     1141 gacggcggcc cagatgagcg cctgcgcgag gttcgaggcc gggcccgcgg ccgccaccca
     1201 gagggaatcg cgcttgggat gccgcaggtt ctggaagttg accggcacgg gcttggccca
     1261 gccgaacatg aagccggaga gcagcagcgt cacgatcggc agcacgatcg tgccgatggg
     1321 atcgatgtgg cgcacggggt tcaggctcac gcgccccagc atgtacgctg tggaatcgcc
     1381 gaagtggcgg gccacgtagc catgggcggc ctcgtgcagg gtgatcgcga aaatcacggg
     1441 cagtgcgtag acgaggatct tgatcaggac gttggtggga tccatcaatg cagcgccgcc
     1501 ttcacgaagc cgaaggggcg gatatcgccg gcgccctcgc gcacgaggcg cggggtttcg
     1561 cccgagaggt cgaccaccgt ggtcacctcg ccatcgcagg cgccggagtc gatcaccagg
     1621 tccacgtggt gctcgaggtg ctggcggatc tcctcggcgt cgttcagcgc cacgccgtgc
     1681 gcgggcagga tgagcgtggt ggaaagcagc ggctcgtcga ggtgctcgag catggccatg
     1741 ggcaccgggt gcgccggaat gcgcacgccg atggtgtggc gcggcgtgag caggcgccgc
     1801 ggcacgtcgc gcgtggcgcg aaggatgaac gtgtagcggc ccggcgtgtt ggcgcggatc
     1861 agccggtatt gcaggttatc caccttcgcg aacaatgaca cctccgaaag gctgcggcac
     1921 atgagcgtga aatggtggcg ctcgtccacg tcgcggatgc gccgcagccg ttccatcgcg
     1981 gccttgtcgc cgatgtggca gccgagcgcg tagcacgagt ccgtggggta agcgaccacc
     2041 ccgccccgcc ggatgatgtc gaccgcggcc cggatgagcc gcggctgcgg attatccgga
     2101 tggaccgtga agaactgcgc catctggtgt gctcgcgcct cctgcggcgc gtcgaaatgc
     2161 ctgtttgccc ttgtcgtgcg ccggccgcta gtgggcccag gacgaccaca ccggcgtgag
     2221 cgatgcgtgc agcgccggga gctttcccgg ctcgcacggg ctctcgcccg gaccatggta
     2281 atcggcgcca cgcgaggcga ggaatccatg ctccagcgcc acgcgctgcc acgccgcgta
     2341 ctgctccggg cggtggctgc ccgtcaccac ctcgatcgcg cgtccgccgg cggcgcggaa
     2401 ctgctgcagc atctcgtcgc gcatcatcgg cttgaggtcg tagcgtcccg gatgcgcgac
     2461 caccgcagtg ccgcccgcgg cgatgatcca cttcaccgca tcgtccagcg cggcccagtg
     2521 atgcggcacg aatgcgggcc ggcccttcgc gagccacttg tcgaacgcgg cctgcgtatc
     2581 cttcaccgcg ccgctctccg ccaggtaacg cgcgaaatgc gtgcggctca cgatgcgctc
     2641 gtcaccggcg agctcgagcg cgccctcgaa cgtattcgcg aagcccatgc gcgtgagcgc
     2701 ttcgccgatc ctgcgtgcgc gcccgacgcg gccggcgcgg acagaatcca gcccgtcgcg
     2761 cagcacgcgc gagtgcggat ccacccgcag gcccacgata tgcagcgtag tctcccccca
     2821 gctcacggaa atctcgacgc ccgggacgaa gcccacgccc agggcccgcg cggccgattg
     2881 cgcctcctcc agcccctcga cggtgtcgtg gtcggtgagc gcgatggcgt ccacgcccgt
     2941 gcgggacgcg agctcgatca gctgggtggg ggacagcaaa ccatccgaac gggtcgaatg
     3001 gttatgcaaa tcaaagtttt ccaaacggga aagattcggg aggcaccaaa aattatagca
     3061 aaattgggta tgcaaccgat cgttttcggc gacgagggcc agctcgccac ggccaccctg
     3121 ctgcgccgct tcgccgcgga gatccgcccg gcggggctcc cccatgccgc ggaggccgag
     3181 cgctggctgc gcacgctcga ctcgctcgac cgccactatg gcggcgccgg cgccctgccg
     3241 ctcgaggacg cggcggacgt gctggccgcc acgctctcgg cgctggcaag gctcgcacag
     3301 gatgcaccgc cggacctgca cggcgctgtg gccgacatca cactcgggac cgccatctgg
     3361 gcgctccgcc acgacatcgt cctggatccc gtggaaccgg tcgtgaat
//
LOCUS       DATDMP010000303         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_824902, whole genome shotgun sequence.
ACCESSION   DATDMP010000303 DATDMP010000000
VERSION     DATDMP010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824902"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VI195_05245"
     CDS             complement(<1..495)
                     /locus_tag="VI195_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823823.1"
                     /translation="MRPRKVVVNDRMQRNYTYYRTEPEGRNFHPEFVPQLTPRQMLAA
                     GVFGGKYMTDCRKEFPASWFAGAKLSPAAHDASLNCFGIRASQPLSVWREKGWIHPAD
                     PRGWFQWYCRYYMGRRCADDARQIKRWKAMRRHLAQIRLGCRRGDLRCRKRQRQALLH
                     WAYDS"
     gene            complement(505..843)
                     /locus_tag="VI195_05250"
     CDS             complement(505..843)
                     /locus_tag="VI195_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY6823824.1"
                     /translation="MSAAVPGVIVTTANEVAGYRIAEYKGVVRGLVVRATSVMSGITG
                     GLKSLLGGNIEEYERVCEQARDEAFRRMVAHARQHGADAVIAMRYDATEFVQGSTEVL
                     AYGTAVKLAR"
     gene            complement(840..1433)
                     /locus_tag="VI195_05255"
     CDS             complement(840..1433)
                     /locus_tag="VI195_05255"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY6823825.1"
                     /translation="MNKARLEAFSDGVIAVIITILVLELKVPHGVEAPALLPLLPVFL
                     SYVLSFVVVGIYWNNHHHMFHAVHHVNGDILWANLHLLFWLSLFPFSTAWMGENHFAT
                     LPVAIYGLVLLGATCAYTVLTRALLRTHDKESLLARGVGSDTKGKISLVIYLSAVALA
                     FLSPWIACALYVLVAAIWLCPDPRFERLLKQAREEIR"
     gene            complement(1456..1833)
                     /locus_tag="VI195_05260"
     CDS             complement(1456..1833)
                     /locus_tag="VI195_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823826.1"
                     /translation="MAKQKIAALAVLAAIILLAPIVEGLATGRVESMSPYDIAGTVLS
                     LVPLYWWYHMDKAEHQYRAGALMNVGIGLVAIVALPIYLVRSRGWRRGATAIALGAAF
                     TGLLYLLEWLGEWIGENLAGSLG"
     gene            complement(1833..>2310)
                     /locus_tag="VI195_05265"
     CDS             complement(1833..>2310)
                     /locus_tag="VI195_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY6823827.1"
                     /translation="GSAWLLLGGAPATGQAASRETRASFIRVLVATVSVGIANFIYSL
                     GLRAGATPGTLITTQAAVVVALSSSFVALTERAIRPSTAALRHAPVAALVLAAAFMLM
                     VESMARGEASVSVPIAQMGFVVTAVLGFLVLGERFTARKGLGIVAAVGALASLAHG"
BASE COUNT          396 a          780 c          777 g          357 t
ORIGIN      
        1 gctgtcgtag gcccaatgca agagcgcctg gcgctggcgc ttgcggcagc gcaggtcgcc
       61 cctgcgacag ccgagcctta tctgcgcgag gtgcctgcgc atggccttcc agcgcttgat
      121 ctggcgtgcg tcgtcggcgc agcgccttcc catgtagtag cggcaatacc actggaacca
      181 gccgcgcggg tcggcgggat ggatccagcc cttctcgcgc catacggaga gcggctgcga
      241 cgcccggatg ccgaagcaat tcaggcttgc atcgtgcgcc gccgggctga gcttcgcgcc
      301 ggcaaaccag ctcgccggga attccttccg gcaatcggtc atgtacttgc cgccgaagac
      361 gcccgcggcg agcatctgcc tgggggtgag ctgcggcacg aactccggat ggaagtttcg
      421 cccttccggc tccgtccggt agtacgtgta gttcctttgc atgcggtcgt tgacgacgac
      481 cttccgtggc cgcacggcag ccggtcagcg cgcgagcttt acggcggtgc cgtaggcgag
      541 cacctccgtg ctgccctgga cgaactccgt ggcgtcgtag cgcatggcga tcacggcgtc
      601 cgcgccgtgc tggcgcgcat gcgccaccat gcggcggaag gcctcgtcgc gcgcctgctc
      661 gcagacgcgc tcgtattcct cgatattgcc gccgaggagc gacttcaggc ctcccgtgat
      721 gccgctcatc acgctggtcg cgcgcaccac gagcccgcgc accacgccct tgtattcggc
      781 gatcctgtac ccggcgacct cgttggccgt cgtcacgatc actcctggaa ccgcagcgct
      841 catcggattt cctccctcgc ttgtttgagc agacgttcga atcttggatc cgggcacagc
      901 cagatcgccg cgacgagcac gtaaagcgcg cacgcaatcc acgggctgag aaacgccagc
      961 gccacggccg aaaggtagat cacgagcgag atcttgcctt tggtatcgct gcccactccg
     1021 cgcgcgagca gcgactcctt gtcgtgggtg cgcagcagcg cgcgcgtgag caccgtgtag
     1081 gcgcacgtcg cgccgagcag cacgaggccg tagatcgcca ccggcagcgt ggcgaaatgg
     1141 ttctcgccca tccacgcggt gctgaaggga aagagcgaaa gccagaacaa gaggtgcagg
     1201 ttcgcccaga ggatgtcgcc gttcacgtgg tgcaccgcat ggaacatgtg gtggtggttg
     1261 ttccaataga tgccgactac cacgaagctc agcacgtagc tcaggaacac cggcaggagg
     1321 ggcagcagcg ccggcgcttc gacgccgtgc ggcactttca gctccagcac gaggatggtg
     1381 atgatcacgg cgatgacgcc gtcgctgaac gcctcgaggc gtgccttgtt catgcggctt
     1441 cgagccgggc cttgttcatc cgaggctccc cgcgaggttt tcgccgatcc actcgcccag
     1501 ccactccagc aggtagagca ggccggtaaa ggccgcgccg agcgcgatcg ccgttgcgcc
     1561 gcggcgccag ccgcgcgagc gaacgagata gatcggcagc gcgacgatcg ccacgagccc
     1621 gatgcccacg ttcatcagcg cgcccgcccg gtattgatgc tcggccttgt ccatgtggta
     1681 ccaccaatat agcggcacga gcgagagcac cgttccggcg atgtcgtacg ggctcatgga
     1741 ttccacccgg ccggtggcga ggccctccac gatcggcgcc agcaggatga tggcggcgag
     1801 gaccgcgagc gcggcgattt tctgcttcgc catcagccgt gcgccaggct cgcgagcgcg
     1861 ccaaccgccg cgacgatgcc gaggcccttg cgcgcggtga accgctcgcc aaggacgagg
     1921 aagccgagca cggcggtgac gacgaagccc atctgcgcga tgggaaccga cacgctcgcc
     1981 tcgccgcgcg ccatgctttc caccatgagc atgaacgcag ccgccagcac gagcgcggcg
     2041 acaggcgcat ggcgcaacgc ggccgtcgaa ggacggatgg cgcgctcggt gagcgccacg
     2101 aacgagctgg aaagcgcgac gacgacggcg gcctgggtgg tgatgagcgt gccgggcgta
     2161 gcgccggcgc gcaagccgag gctgtagatg aaattggcga ttccaaccga cacggtcgcg
     2221 accagcacgc ggatgaacga agcgcgagtc tcgcggctcg cggcctgtcc cgtggccggc
     2281 gcgccgccga ggagcagcca cgcggagccg
//
LOCUS       DATDMP010000304         2105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1824742, whole genome shotgun sequence.
ACCESSION   DATDMP010000304 DATDMP010000000
VERSION     DATDMP010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824742"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VI195_05270"
     CDS             <1..956
                     /locus_tag="VI195_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823828.1"
                     /translation="LRLHARLNEALALRLTVEAELGVARGALEEAESALRALDEQRLA
                     AEQRVQAAREAMEQARLAAQETHVRRAALAEQFAQTGCELATVQAHLPAEAEVGSWEE
                     QLADTRADIERLGSVNLAAIDELREHSQRKEYLDSQFADLSSALDTLAEAMRRIDRET
                     RTRFEDTFERINTGLREKFPRLFGGGHAYLELVGDDKLTAGVAVMARPPGKKNSTIHL
                     LSGGEKALTAVSLVFSIFDLNPAPFCLLDEVDAPLDEHNVGRFCDIVREMSSRVQFVF
                     ITHNKGTMELASQLIGVTMNEPGVSRLVTVDVDEAVRLAAV"
     gene            972..1868
                     /locus_tag="VI195_05275"
     CDS             972..1868
                     /locus_tag="VI195_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEY6823829.1"
                     /translation="MMSELRWALLILGVLFIVALTLWERRRPRQASGVFERMPLRNAG
                     AEAAPRVLREPPLVLPEVRVRDPVATAELPVLETGENAALPQLESTAEPPSPLEAQTA
                     AENEDTVDDLPTLVLDGEPVGEDSVAPPWPDEPASADEPDLREELPQLPAAEPLLVDW
                     PAAHERRILALRLVAPERERFAGRSLRQALAAEGFLLGQFAIFHKPDDERRAVLSAAS
                     LTRPGTFDMDTMDSQHYGGLSLFAVIPGPKPAGQAFDELVFAARNLNERLHGVLQDEQ
                     GTPLTPARIEQLRERLRVGAGS"
     gene            1865..>2105
                     /locus_tag="VI195_05280"
     CDS             1865..>2105
                     /locus_tag="VI195_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823830.1"
                     /translation="MTDAAAARAAQLRAEIAQHDYRYYVLDDPLVSDAEYDRLMQELR
                     ALEAAHPELLTPDSPTQRVSGTPRAGFGAVVHQVPM"
BASE COUNT          321 a          698 c          753 g          333 t
ORIGIN      
        1 tcctgcggct gcacgcgcgg ctcaacgagg cgctcgcgct gcgcctcacg gtggaggccg
       61 agctcggtgt tgcgcgcgga gcgttggagg aagcggagag cgccctgcgc gcgctcgatg
      121 agcagcgcct cgccgccgag caacgcgtgc aggcggcgcg cgaagccatg gagcaagcgc
      181 ggcttgcggc gcaggagacg cacgtgcggc gggcagcgct cgccgagcag ttcgcgcaga
      241 ccggctgcga gttggccacg gtgcaggcgc accttcccgc ggaggccgag gtcggatctt
      301 gggaggagca gctcgccgac acgcgcgccg atatcgagcg gctcggctcg gtgaatctcg
      361 ccgccatcga tgagctgcgc gagcacagcc agcgcaagga atatctcgac agccagttcg
      421 ccgatctctc gtccgcgctc gacactctgg ccgaagccat gcgccgcatc gaccgcgaga
      481 cgcgcacgcg cttcgaggac acgttcgagc gtatcaacac cggcctgcgc gagaagttcc
      541 cgcgactctt cggcggcggc cacgcctatc tcgagctcgt gggcgacgat aagctcaccg
      601 caggggtcgc ggtcatggcg cgtcctccgg gcaagaagaa cagcaccatt catctgctct
      661 ccggcggcga gaaggctctc accgcggtgt cgctggtgtt ttccatcttc gatctgaacc
      721 cggcgccttt ctgcctgctc gatgaggtcg atgcgccgct cgatgagcac aatgtcgggc
      781 gtttctgcga tatcgtgcgc gagatgtcga gccgcgtgca gttcgtgttc atcacccaca
      841 acaaagggac catggagctc gcctcgcagc tgatcggcgt gaccatgaac gagccgggag
      901 tctcacgcct cgtcaccgtc gatgtggatg aggcggtgcg gctggcggcg gtgtgagcgg
      961 atgatcggcg catgatgtcg gagctgcgct gggcgctgct gattctcggc gtcctgttca
     1021 tcgtggcact caccctctgg gagcggcggc ggccgcggca ggcgagcggt gtcttcgagc
     1081 ggatgccgtt gcgcaatgcg ggggcggagg ccgcgccgcg ggtgttgcgc gagccgccgt
     1141 tggtgcttcc ggaagtacgc gtgcgcgatc ccgtggcgac agcggagctg ccggtgctcg
     1201 agacgggcga gaacgcggcg ttgccgcagc tcgaatccac ggctgagccg ccgtcgccgc
     1261 tcgaggcaca aaccgcagcg gagaatgagg acacggtgga tgatctgccg acgctcgtcc
     1321 tcgacgggga acccgtcggt gaggactcag tcgcgccgcc atggcctgac gagccggcgt
     1381 cggcggacga gccggatctg cgcgaggagc tgccgcagct gccggcggca gagccgctgc
     1441 tggtcgactg gccggcggca cacgagcggc gcatcctcgc gctgcgcttg gtcgcaccgg
     1501 agcgtgagcg tttcgccggg cgatcgttgc gccaggcact cgccgcagag gggttcctcc
     1561 tcggccagtt cgcgatcttt cacaagccgg acgatgagcg gcgcgcggtg ctctccgcgg
     1621 ccagcctgac gcgcccggga accttcgaca tggatacgat ggactcgcag cactatggcg
     1681 ggctgtcgct gttcgccgtg atcccgggcc cgaaacctgc gggacaggcc tttgatgagc
     1741 tcgtgttcgc ggcgcgcaat ctcaacgagc gcctgcacgg tgttttgcag gatgagcagg
     1801 gaaccccgct cacgccggcg cgcatcgagc agttgcgcga acggctgcgg gtcggagccg
     1861 gctcgtgacc gatgcggcgg ctgcgcgcgc tgcgcagctg cgggcagaaa tcgcgcagca
     1921 cgactaccgc tactacgtgc tcgacgatcc gctcgtctcc gacgccgagt acgatcgtct
     1981 gatgcaggag ctgcgcgcgc tcgaagccgc gcatcctgag ctcctcaccc cggactcacc
     2041 gacgcaacgc gtttccggca cgccgcgcgc cggcttcggc gcggtcgtgc accaggtgcc
     2101 gatgc
//
LOCUS       DATDMP010000305         2514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_270157, whole genome shotgun sequence.
ACCESSION   DATDMP010000305 DATDMP010000000
VERSION     DATDMP010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270157"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1861)
                     /locus_tag="VI195_05285"
     CDS             complement(158..1861)
                     /locus_tag="VI195_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6823831.1"
                     /translation="MPVLALLLVLGAWLKRRALQKLADPTLVPRLTDSRSARWSAVKV
                     ACVLLGLVFMIVAAARPQWGEKLQVVKGRGIDVVIALDASRSMNATDIPPSRLERAKV
                     QIASLLDNLSGNRVGIVAFAGTAQVMCPLTTDVEAAKLYLDIIDPSNMPVPGTNIQHA
                     VEAAASLFGPVEDASKALILITDGDNLDGDPAQATRLAQADHIRLFAVGVGTPEGSTV
                     PEAGSSGTSYQKDENGKIVMSRLGERLLLVMAKATDGRYFRSESINLDALVGALDQVQ
                     KKAITGGEYVEYEERYQSFLILAFLLLFAGTLISDRRGAWFPDAVRSLRSLVGLLPRG
                     RAARPPSKLALLLALAGLLSTSHAHADVGSSMRRGLELEKQGKLADAAKAYQEALVLE
                     PDNVRIRYDIGRVLYQQQQFADAADHFQLGLLSKQKTVRAHALYNLANSQFKQGRLDE
                     AIATYSLALIQDPSDLEAKQNLELCWKIKQQMQQHPDSSKKQQQQQQQQQQQQQQQQQ
                     QQQQRQQQQQQAQAQPAPKGSIPKDQADRMLQALQSKERENLKKQPKPPPSGAPGGRD
                     W"
     gene            complement(1903..>2514)
                     /locus_tag="VI195_05290"
     CDS             complement(1903..>2514)
                     /locus_tag="VI195_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6823832.1"
                     /translation="DRIGVVVFANGAMTLCPLTLDRDVLKSVIDRLKIGTLDGSRTAI
                     GNGLADAVARLKNSTAKEKVVILLTDGVNNAGEVEPLTAARLAQTYGIKVYCIGVGSQ
                     EPVTVMVNDPFWGQRPQTVQADFDLKTLEDISALTGGHAYTAGDAEALKRIYDEISRM
                     EPTHFKSTHHTVYSEKAGLFLLPAALLFLAGALVPALLWRRLP"
BASE COUNT          364 a          847 c          867 g          436 t
ORIGIN      
        1 atgtcctcgc cgcgcacgct caagacgagc tggaactgct cgcccaggcc gacggtcgtc
       61 tggtccacgg acgccgtgaa ctcgatttcg ccggcgcgcg agacgacagc aaccgacgcg
      121 agcaggaaca cggcgagtgc cggcgcggta acgcgcctca ccagtccctc cctcccggcg
      181 cccccgacgg tggcggcttc ggctgcttct tcaggttctc gcgctccttg ctctggaggg
      241 cctgcagcat gcggtccgcc tggtccttgg ggatcgagcc cttgggcgcc ggttgcgcct
      301 gcgcctgctg ttgttgctgt tgtcgctgct gttgctgttg ttgttgttgc tgctgttgct
      361 gctgctgctg ctgctgttgt tgctgctgtt tcttcgacga gtccgggtgc tgctgcatct
      421 gctgcttgat cttccagcag agttcgaggt tctgcttcgc ctcgaggtcg ctcgggtcct
      481 ggatcagcgc cagcgagtag gtggcgatcg cctcgtcgag cctgccctgc ttgaactggg
      541 agttggcgag attgtagagc gcatgggcgc gcaccgtctt ctgcttggag aggagcccga
      601 gctggaaatg gtccgcggcg tcggcgaact gctgctgctg gtagagcacg cgaccgatat
      661 cgtagcggat gcgcacgttg tcgggctcga ggacgagcgc ttcctggtag gccttggcgg
      721 cgtcggcgag cttgccctgt ttctcgagct ccaggccgcg ccgcatgctg gagcccacgt
      781 cggcgtgggc gtgcgacgtc gagagcaggc ccgcgagtgc cagcagcagc gcgagcttcg
      841 agggcgggcg ggcggcgcgg ccgcgcggca gcaggcccac gagcgagcgc agcgaacgaa
      901 ccgcgtccgg aaaccaggcg ccgcgacgat cgctgatcag cgtgcccgcg aagagcagga
      961 ggaacgccag gatcaggaac gactggtagc gctcctcgta ttccacgtac tcgccgccgg
     1021 tgatcgcctt cttctgcacc tggtccagcg ccccgaccag cgcgtccagg ttgatgctct
     1081 ccgagcggaa gtagcgcccg tcggtggcct tggccatcac cagcagcagc cgctcgccca
     1141 gccgcgacat cacgatcttg ccgttctcgt ccttctggta gctcgtcccc gagctccccg
     1201 cctcgggaac cgtcgagccc tcgggcgtgc ccacgccgac cgcgaacaga cggatgtggt
     1261 cggcctgagc gaggcgcgtc gcctgcgccg gatcgccgtc caggttgtcg ccatcggtga
     1321 tgaggatgag cgccttggag gcgtcctcga cgggcccgaa cagcgacgcg gccgcctcca
     1381 ccgcgtgctg gatgttcgtg ccgggcaccg gcatgttgct ggggtcgatg atgtccaggt
     1441 agagcttcgc cgcctcgacg tcggtcgtga gcgggcacat cacctgcgcg gtgcccgcaa
     1501 acgcaacgat gcccacgcgg ttccccgaga gattgtccag gagcgaggcg atctgcacct
     1561 tcgccctctc gagccggctc ggcgggatgt cggtggcgtt catgctgcgc gaagcgtcga
     1621 gcgcgatcac cacgtcgatt ccgcggccct tcacgacctg cagcttctcc ccccactgcg
     1681 ggcgcgccgc ggccacgatc atgaacacga ggccgagcag gacgcaggcg accttcaccg
     1741 ccgaccagcg cgcgctgcgg ctgtcggtga ggcgcggcac cagtgtcggg tcggcgagtt
     1801 tctggagcgc gcggcgcttg agccaggcgc cgaggacgag caacagcgcc agcaccggca
     1861 ccagcgtgag cagccacagc agctgacgcg ccccccagtg gatcacggaa gcctccgcca
     1921 cagcagcgcg ggcaccagcg cgccggccag gaacaggagc gccgcaggga gcaggaacag
     1981 ccccgccttc tccgagtaca cggtgtggtg cgtggacttg aagtgcgtgg gctccatgcg
     2041 gctgatctcg tcgtagatgc gcttcagcgc ctcggcgtcg ccggccgtgt aggcgtggcc
     2101 accggtcagc gccgagatgt cctcgagcgt cttcaagtcg aagtccgcct gcacggtctg
     2161 cggccgctga ccccagaacg ggtcgttcac catcaccgtg accggctcct gcgagccgac
     2221 gccgatgcag tagaccttga tgccgtaggt ctgggcgagc cgcgccgcgg tgagcggctc
     2281 cacctccccg gcgttgttga cgccatcggt gagcaggatg accaccttct ccttggcggt
     2341 cgagttcttg agcctggcaa cggcatcggc gaggccgttg ccgatcgcgg tgcgcgagcc
     2401 atcgagcgtg ccgatcttga gccggtcgat gaccgacttg aggacgtccc gatcgagcgt
     2461 cagcgggcag agcgtcatcg cgccgttggc gaacacgacg acgccgatgc ggtc
//
LOCUS       DATDMP010000306         2105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1270954, whole genome shotgun sequence.
ACCESSION   DATDMP010000306 DATDMP010000000
VERSION     DATDMP010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270954"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VI195_05295"
     CDS             complement(<1..890)
                     /locus_tag="VI195_05295"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY6823833.1"
                     /translation="MRAVTLEPRKTPTLGLADIAEPPVTDGPILVQSVALGVCGTDHE
                     LIEGHYGEAPPGHARLVIGHESLGRVIEAPDRSGFRSGELVVGIVRHPDPVPCANCAV
                     GEWDLCRNGRYTERGIKQADGFSAERWRSDPGFTVRVSAKLGLAAVLLEPASVLAKAW
                     EHVDHIVRRSRAAPKRALVTGAGPVGLMAALMGMQRALEVHVLDHNDSGPKPDLVRAL
                     GAAYHTRFPAVHFDVVVECTGVPSVIVEAVAAGEPGSVVCLTGLGAGQGAQSFDVATL
                     NQRMVLENRVVFGSVNANRR"
     gene            961..>2105
                     /locus_tag="VI195_05300"
     CDS             961..>2105
                     /locus_tag="VI195_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY6823834.1"
                     /translation="MSYLTETVNVAFAAATLAAALIASAHAVLHKRESRAAALWVAAV
                     WLLPAVGVALYFFLGINRVERRATRMRGRMVRHRSTAQFPASEPGTHFLPLARLMGRV
                     VGRPLLAGNAIEPLVAGVQAFPAMLEAIRGARTSIAMASYIFDGTGIGADFVDALGGA
                     ARRGVDVRVLIDDVDARWSRSTAVKPLRQHGVNVAIFNPPLVPARLHAANLRNHRKIL
                     VIDGALGFTGGLNIDCRYWKPEAPEQAFRDLHFRLRGPVVAHLAEVFADDWQFTTDEP
                     LRGQKWFPQLAPAGEVLARGIEAGPDEGMERMRWAILGGINAGQRSVRVLTPYFLPDQ
                     TLINALNAAAMRGVEVDIVIPAKSDLPHVQWAVFGQIWQVLDHGCRV"
BASE COUNT          312 a          752 c          709 g          332 t
ORIGIN      
        1 tggcgacggt tggcattcac cgagccgaag accacgcggt tttcgagcac catccgctgg
       61 ttcagcgtgg cgacgtcgaa cgactgcgcg ccctgccctg cgccgagtcc cgtgaggcag
      121 accacgcttc ccggctcgcc cgcggccacc gcttcgacga tcacgctggg aacgccggtg
      181 cactccacca cgacatcgaa atgaaccgcc ggaaagcgcg tgtgataagc ggcaccgagc
      241 gcgcgcacga gatccggctt cggtcccgaa tcgttgtggt ccagcacgtg gacttcgagg
      301 gcgcgctgca tgcccatgag cgcggccatc aatccgacag ggcccgcgcc cgttacgagc
      361 gcccgcttgg gcgccgcgcg cgagcgccgg acgatgtgat cgacgtgctc ccacgccttc
      421 gcaaggacgc tggccggctc gagcagcacc gcggcgagcc ccagcttggc gctgacgcgc
      481 acggtgaagc ccgggtcgct gcgccagcgc tcggccgaga agccgtcggc ctgcttgatg
      541 ccgcgctccg tgtagcggcc gttcctgcac aggtcccatt cgcccaccgc gcagttggcg
      601 cacggcaccg gatccggatg gcgcacgatg ccgaccacga gctcgccgct gcggaagccg
      661 ctccgatcgg gcgcctcgat cacccgcccc agcgattcat gcccgatgac cagccgggca
      721 tgccctggcg gcgcctcgcc gtaatggcct tcgatgagct cgtgatcggt gccgcagacc
      781 ccgagggcaa ccgactgcac gaggatcggg ccgtcggtga ctggcggctc ggcgatgtcc
      841 gcaagtccca gcgtaggggt cttgcggggc tcgagcgtaa cggcgcgcat attaagctga
      901 cctgcggcag ggtcgcttag ttcatccggc gcacccgccg tagggattac ccttaggctg
      961 gtgtcgtacc tgacagaaac ggtcaacgtc gctttcgccg ccgcgaccct cgcggcggcc
     1021 ctcatcgctt cggcgcacgc ggtgcttcac aagcgcgaat cgcgcgcggc ggcgctttgg
     1081 gtggcggcgg tgtggctgct gccggcggta ggggtcgcgc tctacttctt cctgggcatc
     1141 aatcgcgtcg aacgccgcgc cacgcgcatg cgcggccgca tggttcgcca ccgcagcacg
     1201 gcgcagtttc ccgccagcga gcccggcacg cacttcttgc cgctcgcgcg cctcatggga
     1261 cgggtcgtcg ggcgcccttt gcttgcagga aatgcgatcg agcctctcgt cgcgggcgtc
     1321 caggcgtttc ccgccatgct cgaggcgatt cggggcgccc gcacctcgat cgccatggcg
     1381 tcctatatct tcgacggtac gggcatcggc gcggacttcg tcgatgcgct aggcggcgcc
     1441 gcgcgccgcg gagtggacgt gcgggtgctc atcgacgacg tggacgcgcg ctggtcgcgc
     1501 tccacggcgg tgaagcctct ccgccagcac ggcgtgaacg tcgcgatctt caacccgccg
     1561 ctggttcccg ccaggctcca tgccgccaac ctgcgcaatc accgcaagat cctggtcatc
     1621 gacggcgcgc tgggcttcac cggcgggctg aacatcgact gccggtactg gaagcccgag
     1681 gcgcccgagc aggcattccg cgacctgcac tttcgcctgc gcggcccggt cgtggcgcac
     1741 ctcgccgaag tgttcgccga cgactggcag ttcacaaccg acgagccgct gcgcgggcag
     1801 aaatggtttc cgcagctcgc gcccgcagga gaagtgctcg cgcgcgggat tgaagccggc
     1861 cccgacgaag gcatggagcg catgcgctgg gccatcctcg gcggcatcaa tgccgggcag
     1921 cgatcggtgc gcgtgctcac gccgtacttc ctcccggacc agacgctcat caacgccctc
     1981 aacgccgccg cgatgcgcgg ggtggaagtg gacatcgtca tcccggccaa gagcgacctg
     2041 ccgcacgtgc aatgggccgt gttcgggcag atctggcagg tgctcgacca tggctgccgc
     2101 gtgtg
//
LOCUS       DATDMP010000307         4049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2159407, whole genome shotgun sequence.
ACCESSION   DATDMP010000307 DATDMP010000000
VERSION     DATDMP010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159407"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VI195_05305"
     CDS             <1..363
                     /locus_tag="VI195_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823835.1"
                     /translation="FIIVVVLGFAAFTWVTLHFSYSDGERAGLLQKFSRRGWICKTYE
                     GEMVLATVATVNAEKFMFTVRDADIAKQLIADIGQHVKVHYEQRKWIPTSCFGDTEYF
                     VTSVRPENPERPQPPAPQ"
     gene            complement(375..1868)
                     /locus_tag="VI195_05310"
     CDS             complement(375..1868)
                     /locus_tag="VI195_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEY6823836.1"
                     /translation="MNELAEPSPAAQLAARKTIFGQPFGLATLFLTEMWERFTYYGIQ
                     AVLILFMVAAASRGGLGLDDKTSSSIFGLFLGSTYLLGLLGGWIADRLLGAQRAVISG
                     GILIAAGSALLALGKDGIFFVGLVVNAMGVGLLKPNVSAIVASLYPEGGARRDAGFSI
                     FYMGINIGAALGSFFVPIAAAAFGWRVGFGLPALFMALGVVQFLWTRSYLGTAGLQPA
                     HEQRGSWTPVIGFLVAVAVLVILSLSGRVRLDAAVLATLATWGYAVLAVAYFVYLLFF
                     AGLSREERRRALVMVALFVASVTFWAGYYQQGGSFNLFAERYTDRHVLGWHMPAGVLQ
                     AVNPSFVIIFAGLFAALWIALGKRGRDLTAAAKFGIALILLGLGFLVMYVAAQRVLAG
                     AMVLPTWLICTYFLHTCGELCISPVGLSTMSKLAPPRFVGQAMGVWFLSLALGGNLAG
                     QLTGQYDASHLDSLPALFLRIFAYGVIAGAVMLLLTPLLKRMMSGVR"
     gene            complement(1865..2629)
                     /gene="tatC"
                     /locus_tag="VI195_05315"
     CDS             complement(1865..2629)
                     /gene="tatC"
                     /locus_tag="VI195_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY6823837.1"
                     /translation="MSEEPEKLAEGTLISHLLELRDRLLRALVAVGLAFLPCVYYSND
                     LFSFMAQPLLAKLPQGSNLIATGVMSPFTTPFKLSFFAALLLAMPYVIYQLWAFVAPG
                     LYRHERRFAVPLLVSSIILFYVGIAFAYYFVFPVMFEFFAHTTPKGVAMMTDINNYLD
                     FVLTMFFCFGLAFEVPIAVVLLVLMGIVRIESLKKNRGYVLIGIFILAALLTPPDAIS
                     QCSLAIPMYLLYEGGILMARVLSRSHSPEAQTKTEQ"
     gene            complement(2613..3065)
                     /gene="tatB"
                     /locus_tag="VI195_05320"
     CDS             complement(2613..3065)
                     /gene="tatB"
                     /locus_tag="VI195_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY6823838.1"
                     /translation="MKVRTRNSPKSRRRATPRPNGARDLFEGRFSEILIIFVLALIVL
                     GPEKLPRVVSEVGRWVGRARAMARQFREQLEEEVQLEQARKAQRPTPASAPEPPTAAA
                     PSVAPRGNGHSPPVTPDTTHRDPPADTTAADATATRPAEAGGTDERRT"
     gene            complement(2995..3225)
                     /gene="tatA"
                     /locus_tag="VI195_05325"
     CDS             complement(2995..3225)
                     /gene="tatA"
                     /locus_tag="VI195_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY6823839.1"
                     /translation="MRELVIILLVVLVVFGAKKLRTVGSDLGHAVRGFKKAMSEGEAE
                     NNAPPKQIRSEGTDAEFPEVAKKSDTKTERST"
     gene            complement(3359..3799)
                     /gene="dtd"
                     /locus_tag="VI195_05330"
     CDS             complement(3359..3799)
                     /gene="dtd"
                     /locus_tag="VI195_05330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY6823840.1"
                     /translation="MIALIQRVSAAQVRVGAELVGEIERGVLALIGVQRGDTASSAER
                     LLERILSYRVFPDAEDRMNLSLREVGGGLLLVPQFTLAADTHKGTRAGFSSAAEPGEA
                     QRLFAGLVGCARGLYAPVSAGVFGAHMQVSLVNDGPVTFWLEAR"
BASE COUNT          809 a         1305 c         1313 g          622 t
ORIGIN      
        1 ttcatcattg tcgtcgttct cggcttcgcg gccttcacct gggtgacgct gcacttttcg
       61 tattccgacg gcgagcgtgc cgggttattg cagaagttct cccgccgggg atggatctgc
      121 aagacgtacg aaggcgagat ggttctggca accgttgcca cagtgaatgc cgaaaagttc
      181 atgttcaccg tgcgcgatgc ggacattgcg aagcagctca tcgccgacat cggccagcac
      241 gtgaaggtgc attacgagca gcgcaagtgg atcccgacct catgctttgg cgataccgag
      301 tacttcgtga cgagcgtgcg gccggaaaac cccgagcgac cgcaaccgcc ggcacctcag
      361 tagcgccggt gatctcagcg cacgccgctc atcatccgtt tcagcaacgg agtgagcagc
      421 agcatcaccg cgccggcgat cacgccgtac gcgaagatcc gcagaaacag cgccgggagt
      481 gagtcgaggt ggctggcgtc gtactgcccg gtgagctgac cggcgaggtt gccaccgagc
      541 gcgagcgaca ggaaccacac acccatcgcc tggccgacga agcgcggagg cgcgagcttg
      601 ctcatggtgg ataacccgac gggtgagatg cacagctccc cgcaggtgtg cagaaaataa
      661 gtgcatatga gccaggtggg cagcaccatc gcaccggcga gcacgcgctg cgccgccaca
      721 tacatgacga ggaaaccgag cccgaggagt atcagcgcaa tgccgaactt ggccgctgcc
      781 gtgagatcgc ggccgcgttt gccgagtgcg atccacaacg ccgcaaacaa gcccgcgaag
      841 atgatcacga aggagggatt taccgcctgc agcactccgg ccggcatgtg ccagccgagg
      901 acgtggcggt cggtgtagcg ctcggcgaac aggttgaagg agccaccctg ctggtagtag
      961 cccgcccaga aggtcaccga tgcgacgaac agcgccacca tgacgagcgc gcgcctgcgc
     1021 tcctctctcg agagtcccgc gaagaacagc aggtacacga agtacgccac cgcgagcacc
     1081 gcgtagcccc aggtggccag cgtggccagc accgcggcat cgagtctcac ccgcccgctg
     1141 aggctgagga tcacgagcac ggcaaccgcg acgagaaacc cgatcaccgg tgtccacgag
     1201 ccgcgctgct cgtgcgcggg ctgcaatccg gccgtgccga ggtaagagcg cgtccacagg
     1261 aactgcacca cgccgagtgc catgaagagc gcgggcagcc cgaatcccac gcgccagccg
     1321 aaagccgccg cggcgatggg aacaaagaac gatccgagcg ccgcgccgat gttgatgccc
     1381 atgtagaaaa tagagaagcc cgcatcgcgc cgcgcgccgc cttccggata gagcgacgcg
     1441 acgattgcgc tcacattggg cttgagcagc ccgacgccca tggcgttcac gacgaggccg
     1501 acgaagaaga ttccgtcctt accgagcgcg agcagcgcgc tgccggcggc gatgagtatt
     1561 cctccgctga tcaccgcccg ctgcgcgccg agcagacgat cggcgatcca gcccccgagc
     1621 agtcccagca gataagtgct gccgaggaag aggccaaata tcgagctcga ggtcttgtcg
     1681 tcgagtccca ggccgccgcg cgatgccgcc gccaccatga acaggatgag taccgcctgg
     1741 atgccgtagt aggtgaagcg ctcccacatc tcggtgagaa acagcgtcgc cagtccgaac
     1801 ggctggccga aaatcgtctt gcgcgctgcc agctgcgctg caggggacgg ctcggcgagc
     1861 tcgttcattg ttcggtcttg gtttgtgctt ccgggctgtg actgcgcgac agcacgcgcg
     1921 ccatgagaat gccaccctcg tacaggaggt acatcgggat ggcgagggag cactgcgaga
     1981 tggcatcggg cggagtgagg agcgcggcga ggatgaagat gccgatcagc acgtagccgc
     2041 gattcttctt gaggctctcg atgcgcacga tgcccatcaa cacgagcaac accaccgcaa
     2101 tgggcacttc gaacgcgagt ccgaagcaga agaacatggt gagtacgaaa tcgagatagt
     2161 tgttgatgtc ggtcatcatc gcgacaccct tcggcgtcgt atgagcgaag aactcgaaca
     2221 tcaccggaaa gacgaagtaa taggcgaagg cgatgccgac atagaacagg atgatggatg
     2281 agacgagcag cggtaccgcg aagcgccgct cgtgacggta caggcccgga gcgacgaacg
     2341 cccacagctg atagatgacg tacggcatcg ccagcagcaa cgccgcgaag aacgacagct
     2401 tgaagggcgt ggtgaagggc gacatcacgc cggtggcgat gagattgctc ccctgcggaa
     2461 gcttggcgag cagcggctgc gccatgaagc tgaacagatc gttcgagtaa tagacgcacg
     2521 gcaagaaggc gagccccacc gcgacgagcg cccgcagcag gcgatcccgc agctccagca
     2581 ggtgtgagat cagtgtgcct tcggcgagct tttcaggttc ttcgctcatc ggtgccgccc
     2641 gcctcggcgg gtcgcgtagc ggtggcgtcg gcagcggtgg tgtcagcggg cggatcgcgg
     2701 tgagtggtgt cgggagtcac cgggggcgag tggccgttgc cgcgcggcgc cacggagggc
     2761 gcggcggcgg taggcggctc gggggcagag gccggcgtgg ggcgctgagc cttgcgggcc
     2821 tgctcgagct gcacttcctc ctcgagctgc tcgcggaact gacgcgccat ggcacgggcc
     2881 cgtcccaccc agcggcccac ttctgacacc acgcgcggca gcttctccgg tcccagcacg
     2941 atcagtgcca ggacgaagat gatcaggatc tccgagaacc ggccttcgaa cagatcacgt
     3001 gctccgttcg gtcttggtgt cgctcttctt cgcgacttcg gggaattccg cgtccgtacc
     3061 ttcagagcgg atctgtttcg gcggggcgtt gttctccgcc tcgccctcgc tcatcgcctt
     3121 tttgaacccg cgcaccgcgt gtccgaggtc cgagccgacg gtccgcaact tctttgcacc
     3181 aaacacgacg agcaccacga gcagaatgat caccagctcg cgcattccta aaccgcccat
     3241 acttcgcgac tccacaagct tccgcctgcg cggcggaaaa gaaccttagg ataagtcaaa
     3301 ggagtgcccg gagccccata agttcgcaca tcggcgcgac gcacttcacg cttgccggtc
     3361 agcgggcctc caaccagaag gtcaccggcc cgtcattgac cagcgagacc tgcatgtgtg
     3421 ccccaaatac acctgcggaa acaggggcat agagcccacg agcgcacccc accagcccgg
     3481 cgaacaaccg ctgcgcctcg cccggttccg cggcgctgct gaaccccgcg cgcgtgcctt
     3541 tgtgggtatc cgccgcgagc gtgaactgcg gcacgagcag cagtcccccg cccacctctc
     3601 gcagggagag gttcatgcgg tcttcggcgt ccggaaagac ccggtaggag aggatgcgct
     3661 cgagcaaccg ctcggcggat gaggcggtgt caccgcgctg cacaccgatg agcgcgagca
     3721 ctccgcgctc gatctcgccg acgagctcgg ctccgacgcg cacttgcgcc gcgctcacac
     3781 gctggatgag ggcaatcatg cttcaagctt acgacagccg ctcctgcgca aggactaaca
     3841 ggggcgcgct cagtggcttt tgcggggcgg agcccgtaac attcggggcc gttttttcca
     3901 ctcgccggag aacaggatgg ggcgtcggtg cggccctgcc gggcagatcg ttccggtcgc
     3961 ggcactcgta gcgtgtgagc tgacgggcgt cggcttcgca cagactccga caactgcgcc
     4021 cgcgcccgcg cagccggcgg ctgcacccg
//
LOCUS       DATDMP010000308         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1492071, whole genome shotgun sequence.
ACCESSION   DATDMP010000308 DATDMP010000000
VERSION     DATDMP010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492071"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VI195_05335"
     CDS             complement(<1..712)
                     /locus_tag="VI195_05335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY6823841.1"
                     /translation="MPWKRRPFRRGPLSRALNIADLRELARRRVPGFAFEYVEGGAED
                     ECTLRGNREALARLRLVPQTLIDTSGRHLRTTIIGRAAEAPLVIAPTGLNNLLHPRGD
                     LALARAAARRGIPFTLSSLSTTRLEDVATRAGGRLWMQLYVLKDRAIARDIMQRAGAA
                     GYEALVFTTDANVFGSREWDQRSYRAPGRPTLRTVLDALRHPHWMTEVLLRNGIPRFR
                     NVESFLPPGAGSAVGGSTV"
     gene            complement(738..>2097)
                     /locus_tag="VI195_05340"
     CDS             complement(738..>2097)
                     /locus_tag="VI195_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823842.1"
                     /translation="NTVNLSASPLSVTVQPFMTATPAPVDSTLLRARGLLVTTQQPTA
                     SAFIMNVRPFTDLVSALGALTVNTSASTYFNLNGVAYTGAAGLAAMGSLQVSSPVAAF
                     GTLADLSGITPTFNATAVYAGISQESLLTDSIRGIVAARSGNTLTIHGASFVPRPSAG
                     LFTSLQYLNNATVNIGSGTVVSEDGANVAGLTTQSVSIGQEVTVFGAALYNSSGVFAS
                     LDATGGPVPAQLRLAQTPIWGTLNTATTGSVSLNLLSLGDFEPGALSFAGTGISSAND
                     ALPASYQVDTGTIDESATPAGTLLEASGVVSAFGAAPPDFTATTVTPGSAVTQQLVVE
                     WINGGAATPFSSLSGSGLVVDLSNAHLGTTHYIATGPTQVDITTLPASPQIAFGSGFL
                     TLAYGGKGSTSVFNGASAFATGLAGALTGTNAVYRLVCVGQYSAATNTFTATRVSVAF
                     QG"
BASE COUNT          303 a          660 c          825 g          309 t
ORIGIN      
        1 tgacggtgct gccgcccacg gcgctgccgg cgccgggcgg caggaacgac tccacgttgc
       61 ggaagcgggg gatgccgttg cgcagcagca cttcggtcat ccagtgcgga tggcggagcg
      121 catcgagtac ggtgcgcagc gtgggccgtc cgggcgcgcg gtagctgcgc tgatcccact
      181 cgcggctgcc gaacacgttg gcgtcggtag tgaacaccag cgcctcatag ccggcggcgc
      241 cggcgcgctg catgatgtcg cgcgcgatgg cgcggtcctt gagcacgtag agctgcatcc
      301 acagccgtcc gccggcccgc gtcgccacgt cctcgagccg cgtggtggag agcgagctca
      361 gtgtgaacgg aatgccgcgc cgcgccgcgg cgcgcgccag cgcaaggtcg ccgcgcgggt
      421 gcagcaggtt gttgaggccc gtgggtgcga tgacgagcgg cgcctcggcg gcgcggccga
      481 tgatggtggt gcgcaggtgg cggccggagg tgtcgatgag cgtttgcggc acgaggcgca
      541 gccgcgccag cgcctcgcgg ttgccccgca gcgtgcactc gtcctcggcg ccgccctcga
      601 cgtactcgaa ggcgaagccc ggcacgcggc ggcgcgcgag ctcgcgcaga tccgcgatgt
      661 tgagggcgcg ggacagcggg ccgcggcgga atgggcgccg tttccagggc atgtgcccgg
      721 ccccgcggcg ctgccgccta gccctggaac gccacgctca cccgcgtggc ggtgaaggtg
      781 ttggtggcgg ccgaatactg gccgacgcac accaggcggt acacggcatt ggtgccggtc
      841 agtgcgccgg cgagcccggt ggcaaaggcg ctggcgccgt tgaagaccga ggtgctgccc
      901 ttgccgccgt acgccagcgt gaggaaaccg gagccgaagg cgatctgggg gctcgccggg
      961 agcgtggtga tatccacctg tgtcggcccg gtcgcgatgt agtgcgtcgt gccgagatgc
     1021 gcattgctga ggtcgaccac cagccccgag ccgctcaggc tcgagaaggg agtcgccgca
     1081 ccgccgttga tccactcgac caccagctgc tgcgtgacgg cgctgccggg ggtgaccgtc
     1141 gtggcggtaa agtccggcgg cgcggcgccg aaggcgctga cgacgccgct cgcctcgagc
     1201 agcgtgccgg cgggcgtcgc gctctcgtcg atcgtgccgg tgtcgacctg gtagctggcc
     1261 ggcagcgcat cgttggcgga gctgatgccg gtgccggcga agctgagcgc gcccggctcg
     1321 aagtcaccga gcgagaggag gttgagcgac acgctgccgg tggtggcggt gttgagcgtg
     1381 ccccagatcg gggtctgggc gagccgcagc tgcgcgggca ccgggccgcc ggtggcatcg
     1441 agcgacgcga agacgccgga ggaattgtac agggctgcac cgaaaacggt gacttcctgt
     1501 ccgatagaca ccgactgcgt cgtcagtccg gcgacattcg ctccgtcctc gctcacgacc
     1561 gtgcccgagc cgatgttgac ggtggcgttg ttgaggtact ggagcgaggt gaacaggccc
     1621 gcgctcggcc gcggcacgaa gctcgcgcca tgaatggtga gagtgttgcc gctgcgcgcg
     1681 gcgacgatgc cccggatcga atcggtgagc aggctctcct ggctgatgcc ggcgtagacg
     1741 gcggtggcgt tgaaggtcgg cgtgatgccg gagaggtcag cgagcgtccc gaacgccgcc
     1801 accggggagc tcacctgcag tgatcccatg gccgcgagcc cggcggctcc ggtgtaggcg
     1861 acaccgttga ggttgaagta tgtggaggcg ctggtgttca cggtgagcgc gccgagcgcc
     1921 gagaccaggt cggtgaacgg ccgtacattc atgatgaagg cgctggcggt gggctgctgg
     1981 gtggtcacca gcaggccgcg ggcgcggagc agcgtgctgt cgaccggcgc cggggttgcg
     2041 gtcatgaaag gctgcacggt gacgctgagc ggcgaggcgc tcaaattaac ggtgttg
//
LOCUS       DATDMP010000309         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_604045, whole genome shotgun sequence.
ACCESSION   DATDMP010000309 DATDMP010000000
VERSION     DATDMP010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604045"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2171
                     /locus_tag="VI195_05345"
     CDS             <1..>2171
                     /locus_tag="VI195_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6823843.1"
                     /translation="TPPLVIRYGATDVPIIQLALSSTSLPDSKLNDLGQNLIRPALAV
                     VHGAQVPYPYGGKPRVIMADLNSSALQARGLTPADVSNALETQNVILPAGDVKIGNFD
                     YLVSMNNSPDVIDAINSFPIKWVDGREVFMRDVAHVHDGFQVQTNSVSVDGVPGALMT
                     IRNTGGVSTLAVINGIRAVLPEIRHMLPHDVRIHAIFDQSVFVKAALNSVLMGGAMAA
                     ALTALMILLFLGNWRLTVMILAAIPLSIMSAVLLMGAAGETLNTMTLGGFALAVGILV
                     DNGTVVIENIERNITLRAHLADAIVHGCGEVGTPTFLSTLCICIVFVPVFLLQGTAKY
                     LFSPLSVSVIVSLLASLALSFTLVPVLFFYLMRAHGRAGQGGARSSHRTAAAAAAAAG
                     AHGADVHPFMRLHLAFEHGFRRFREAYREGLAWALAEPRLIVLAFLGIMLVSLPLFPL
                     LGRDFFPDVDAGQMRLHVRAPAGTRIETTQQYFAQVEASIRRLIGNDEIGVILDNIGL
                     PYSGINIALSDSATVGPMDGEILVSLQKKHAPTAQLTAMLRRELPQRFPALQFFFQPS
                     DIVDQVLNFGQPAPIDIRVSAADTESAYKLAARLSRLIERTPGVVDAHVFQVPNAPAL
                     SVDVDRALATQVGLTQREAASNVLVTTNSSAQTSPNFWVDPSNGVSYPLVVQQPPYDI
                     ASAQDLKIMPVSSAAGASQSQLLMNMSSIGRQTAPLVTSQL"
BASE COUNT          342 a          736 c          691 g          402 t
ORIGIN      
        1 caccccgccg ctggtaattc gctacggcgc gaccgacgtg ccgatcatcc agctggcgct
       61 ctcgagcacc tcgctgcccg acagcaagct caatgatctc ggccagaacc tcatccgtcc
      121 ggcactcgcg gtagtgcacg gcgcgcaggt gccgtatccc tacggcggca agccccgcgt
      181 catcatggcc gatctcaaca gctccgccct gcaggcgcgc gggctcaccc cggcggacgt
      241 gagcaacgcg ctcgagacgc agaacgtgat cctgcccgcc ggggacgtga agatcggcaa
      301 tttcgactac ttggtgtcga tgaacaacag ccccgatgtc atcgacgcca tcaactcctt
      361 cccgatcaag tgggtggacg ggcgcgaagt gttcatgcgc gacgtggcgc acgtgcacga
      421 tggcttccag gtgcagacca actcggtatc ggtggatggc gttccgggag cgctcatgac
      481 catccgcaat accggcggag tctcgacgtt ggccgtgatc aacggcatcc gcgcggtgct
      541 gcccgagatc cgccatatgc taccccacga cgtgcgtatc cacgcgatct tcgatcagtc
      601 ggtgttcgtc aaggcggcac tcaacagcgt gctgatgggc ggcgccatgg ccgccgcgct
      661 gaccgcgctc atgatcctgc tgttcctcgg caactggcgc ctgacggtga tgatcctggc
      721 ggcgatcccg ctgtcaatca tgagcgccgt gctgttgatg ggggcggcgg gagagacgct
      781 caacaccatg acgctcggcg gcttcgcgct ggcggtcggg atcctggtgg acaacggcac
      841 cgtggtgatc gagaacatcg agcgcaacat cacgttgcgc gcgcatctcg ctgatgccat
      901 cgtgcacggc tgcggcgagg tgggcacgcc gaccttcctg tcgaccctgt gcatctgcat
      961 cgtgttcgtg ccggtgttcc tgctgcaggg cacggcgaag tacctgttct cgccgctctc
     1021 cgtgtcggtg atcgtctcgc tgctggcgag tctggcgctc tcattcacgc tcgttccggt
     1081 gctgttcttt tatttgatgc gtgcgcacgg gcgtgcgggg cagggtggcg cccgctccag
     1141 ccaccgcacg gccgcggcgg ccgccgccgc cgcgggtgcg catggcgcag atgtgcatcc
     1201 gttcatgcgt ttgcacctgg cattcgagca cggcttccgg cgctttcgcg aggcgtaccg
     1261 cgaggggctg gcctgggcgc tcgccgagcc gcggctcatc gtgctggcgt ttctcggcat
     1321 catgctggtc tcgctgccgc tgtttccgct gctcgggcgc gatttcttcc ccgacgtcga
     1381 tgccgggcag atgcgcctgc atgtgcgcgc gccggcgggt acgcgcatcg agactaccca
     1441 acaatacttc gctcaggtcg aggcgagcat ccgccggctg atcggcaacg atgagatcgg
     1501 cgtgatcctc gacaacatcg gcctgcccta cagcggcatc aacatcgcgc tctcggactc
     1561 cgcaaccgtc ggcccgatgg atggggaaat cctggtctct ctgcagaaaa agcatgcgcc
     1621 gaccgcgcag ctcaccgcca tgctgcgccg cgagttgccg cagaggtttc cggcgctgca
     1681 attcttcttc cagccctccg acatcgtcga ccaggtgctg aacttcgggc agccggcgcc
     1741 gatcgacatc cgtgtcagcg ccgccgacac cgagagcgcc tacaagctgg ccgcgcggtt
     1801 gtcacggctg atcgagcgca caccgggcgt ggtggatgcg cacgtgttcc aggttcccaa
     1861 tgcgccggcg ctgtcggtcg atgtcgatcg cgcgctcgct acccaggtgg gactcaccca
     1921 gcgcgaggcc gcgagcaacg tgctggtgac caccaattcg agtgcacaga cttcgccgaa
     1981 cttctgggtc gaccccagca acggtgtgag ctatccgctg gtggtgcagc agccgcccta
     2041 cgacatcgcc agcgcgcagg atctgaagat catgccggtg agcagcgccg ccggcgccag
     2101 ccagtcgcag ctgttgatga atatgtcctc tatcggccgt cagaccgccc cgctggtcac
     2161 ctcgcagctg a
//
LOCUS       DATDMP010000310         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1492498, whole genome shotgun sequence.
ACCESSION   DATDMP010000310 DATDMP010000000
VERSION     DATDMP010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492498"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1027
                     /locus_tag="VI195_05350"
     CDS             185..1027
                     /locus_tag="VI195_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY6823844.1"
                     /translation="MHDLQIGWGDVLHHLHPDTPIGALVYLALFVLVALLLSRLLRVT
                     VRGALVRRAHLDRTTMVFLQQIGIVAIWVCMLILYAHLIPVLRSLGTALLTGASIASV
                     VIGLAAQSTLGNLVAGVAITLYRPFHLGDRLQVTAPTGTEVGSVEMLSLGYTTLRTDD
                     GRLVVLPNSVAASQVVINLSASQPRAAAPLSISIHVSPATDVGAARELALRTAGATLG
                     EAALIGCFLTRMDDSGATLELRLRAPAAAERDALRSKLLEQLSRGFASSGLDPKDGKR
                     ASFS"
     gene            1089..1667
                     /locus_tag="VI195_05355"
     CDS             1089..1667
                     /locus_tag="VI195_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY6823845.1"
                     /translation="MNRVVSFRHMEDGTREDYLLLDESERRYAQGLPERVLECMRKLD
                     HSLYGYPVTRLQHSLQAATRALRDGADDELTAAALLHDIGDELAPYNHSEMAAALLRP
                     YVRAEVAWIVEHHGLFQNYYYVHHLGGDRHARDRLREHPWYAGCVHFCAAWDQCSFDP
                     DYASESLEFFVPLVQRIFTRAPHDPRYQSALA"
     gene            complement(1649..>2877)
                     /locus_tag="VI195_05360"
     CDS             complement(1649..>2877)
                     /locus_tag="VI195_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY6823846.1"
                     /translation="AVTAAPRWQRLAHRFDRQRLLVPLAAGRDELEGLHANTTIPQII
                     GAARGFEQSGIPELRAAAETFWQTVAQRRAYCTGGTSNGESWNTPPGILAHELSGYTE
                     ESCVTYNMQKLTRLLFGWSADARLADYYERALYNGILGVQHPADGDKLYYLPLESGYW
                     KLFGTPLQDFWCCTGSMAESFAKLGDSIYFQDEAGVYVNLYVASELVWAERQLRLVLD
                     TQFPHEDTVRLIAHTARPQRLRLRVRVPYWTAAGAAQLNGEPLPTPATPGSYFQLERR
                     WRDGDALSVRLPMHLHLAHMPDDASLQAVMYGPLVLAGRMGTVGLDAARLRAEPTAPR
                     RVPEYRAPPLAAPHIVASAADPASWLEPLPSRPLAFRTTGQAQPMTLEPLNGIFDERY
                     AVYWRVLAGAAQASAL"
BASE COUNT          431 a          974 c          992 g          480 t
ORIGIN      
        1 cggcgcgccg cgtggcggcc ggcccggcca gcagcagcgc cgcagcgccc aacagatcgc
       61 gacgattgag actcatggcg tcctcccccg gtgcgggaag atacaccgtg gacgtgggcg
      121 cgactacgtg gcgcgcatcg ccggcagcgg gccgggagca ccggccgtgc tatgttggga
      181 aatcatgcac gatctgcaaa tcggctgggg cgacgtcctg caccacctgc accccgatac
      241 gcccatcggt gcgctggtgt atctggcgct tttcgtgctg gtggcactgc tgctgtcgcg
      301 tctgctgcgc gtcaccgtgc gcggcgcgct ggtgcgccgc gctcacctcg atcgcaccac
      361 catggttttc ctgcagcaga tcggcatcgt cgccatctgg gtgtgtatgc tcatcctcta
      421 tgcgcacctc ataccggtgc tgcggtcgct cggcacggcg ctgctcaccg gtgcgagcat
      481 cgcttccgtc gtcatcgggc tcgcggcgca gagcacactc ggcaatctcg tcgccggagt
      541 cgccattacg ctctaccggc cgtttcacct gggcgatcgg ctgcaggtca cggcgccgac
      601 cggtaccgaa gtcggcagcg tcgagatgct ctccctcggc tacaccacgc tgcgcaccga
      661 cgatggccgt ctggtggtgt tgcccaacag cgtcgccgcc agccaggtgg tcatcaacct
      721 gagtgcctcg cagccgcgtg ccgcggcgcc gctctcgatc agcattcacg tgagccccgc
      781 gaccgatgtc ggggcagcgc gtgagctcgc gctgcgcact gcgggtgcca cgctcgggga
      841 agcggcgctc atcggttgct tcctgacccg catggatgac agcggcgcca cactcgagct
      901 gcgcctgcgc gctccggccg cggccgaacg tgacgcgctg cgctcgaagc tgctcgagca
      961 gctctcgcgc ggcttcgcat cgagcgggct ggatcccaag gacggcaagc gcgcgagctt
     1021 ctcatgaggc gcgctcgaca gcgccctgcg tggacagggc tttggtggcg aagctagact
     1081 cgcagcgcat gaacagagtg gtcagcttcc ggcacatgga ggacggcacg cgcgaggatt
     1141 acctgctgct cgatgagtcc gagcgccgct atgcgcaggg tctgccggag cgcgtgctcg
     1201 agtgcatgcg caagctcgat cattcgctgt acggctaccc ggtaacgcgt ctgcagcatt
     1261 ccctgcaggc cgccacgcgc gcactgcgcg atggagcgga cgatgagctc acagctgccg
     1321 cgctgctgca cgacatcggc gatgagctcg cgccctacaa tcattccgaa atggccgcgg
     1381 cgctcctgcg gccgtatgtg cgcgcggagg tggcctggat cgtcgagcat cacgggctct
     1441 tccagaacta ctactacgtg catcacctcg gcggcgaccg ccacgcgcgt gaccggctgc
     1501 gcgagcatcc gtggtatgcc ggctgtgtgc acttctgtgc cgcctgggat caatgctcct
     1561 tcgatcccga ctatgccagc gagagcctgg agttcttcgt gccgctggta cagcgcatct
     1621 tcacgcgcgc gccgcacgat ccgcgctatc agagcgcgct cgcctgagcg gcgcccgcca
     1681 gtaccctcca gtagaccgcg tagcgctcat cgaagatgcc gttgagcggc tcgagcgtca
     1741 tcggctgcgc ctggccggtg gtgcggaagg cgagtggacg gctcggcagt ggctcgagcc
     1801 aactcgcggg atccgcagcg gaggcgacga tgtgtggcgc tgccagcggg ggcgcgcggt
     1861 actcgggaac ccggcgtgga gcggtgggtt cggcgcgcag gcgcgccgca tcgagcccga
     1921 cagtacccat gcgtccggcg agcaccagcg ggccatacat caccgcctgc agcgaggcat
     1981 cgtcgggcat gtgtgccagg tgcaggtgca tcggcaggcg cacgctgagc gcatcgccgt
     2041 cgcgccagcg gcgctcgagt tggaaatagc ttccgggagt ggcgggagtg ggcagcggct
     2101 cgccgttgag ctgcgcggcg ccggcggcgg tccaatacgg aacccgcacg cgcagcctga
     2161 ggcgctgcgg gcgcgccgta tgcgcgatga gccgcaccgt gtcctcgtgc gggaactgcg
     2221 tgtccagcac taaccgcagc tgccgctcgg cccacaccag ctcggaggcg acgtacaggt
     2281 tcacgtacac gcccgcctcg tcctggaagt agatgctgtc gccgagcttg gcgaaggatt
     2341 ccgccatgct gccggtgcag caccagaagt cctgcagcgg cgtgccgaag agcttccagt
     2401 aaccgctctc gagcggcagg tagtagagct tgtccccgtc ggccggatgt tgtacgccga
     2461 ggatgccgtt gtagagcgcg cgctcataat aatcggccag gcgcgcgtcc gcgctccagc
     2521 cgaacagcag ccgcgtcagc ttctgcatgt tgtaggtgac gcacgactcc tcggtgtagc
     2581 cggagagctc gtgcgccagt atcccgggcg gggtgttcca gctctcgccg ttgctggttc
     2641 ccccggtgca gtaggcgcgc cgttgcgcga ccgtttgcca gaaggtctcg gccgccgcgc
     2701 gcagctcggg tatgccgctc tgctcgaagc cacgcgcagc gccgatgatc tgtggaatgg
     2761 tggtgttggc gtgcagacct tccagctcat cgcggccggc ggcaagcggc acaagcagac
     2821 gctgccggtc gaagcgatgc gcgaggcgct gccagcgggg agcggccgtc actgccg
//
LOCUS       DATDMP010000311         3350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_492822, whole genome shotgun sequence.
ACCESSION   DATDMP010000311 DATDMP010000000
VERSION     DATDMP010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492822"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VI195_05365"
     CDS             complement(<1..408)
                     /locus_tag="VI195_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY6823847.1"
                     /translation="MNAAVSAMSYLGAGYSLRSWLTTRDHKRIAILYAVSITAFFFIG
                     GAAATLMRLELLSPHGTLVTAETYNKLFTAHGVIMVWFFLIPSIPATLGNFLVPLMIG
                     AHDLAFPRLNLASWYIYMAGGIIVLASLALGGVD"
     gene            complement(405..1373)
                     /gene="coxB"
                     /locus_tag="VI195_05370"
     CDS             complement(405..1373)
                     /gene="coxB"
                     /locus_tag="VI195_05370"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY6823848.1"
                     /translation="MSAVLAMPPAASELATHVDALLWSVTAITATVAATIMVLLVIFC
                     IRYRRGAAVDRTMRDAAGEHRRWLEVTWITVPFLVFLGFFFWGARLYFSYESPPRSPL
                     QIYVVARQWMWKIEHPTGAREINELHVPRGRPVKLVMTSQDVIHSFYVPAFRVKRDVV
                     PGRFAVLWFTATRSGVYHLLCAEYCGTDHSRMGGRIVVLEPDAYARWLESGSHESSLA
                     SRGAAKFQNFGCSGCHVGGAAVHAPDLSHLYGRTIPLADGSFVRVDERYLRDSILLPG
                     KEIAAGYADQMPSFAGRATDEDLLELIEYIQSLAPQATVPGAEDSP"
     gene            complement(1370..2173)
                     /locus_tag="VI195_05375"
     CDS             complement(1370..2173)
                     /locus_tag="VI195_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY6823849.1"
                     /translation="MTARLAALIGLAASCCLVLPAQATTVTDIQQRVGLDQHLGRELP
                     LDVTLRDERGTPVKLGELLGARPAVIAPVYFECPNLCTLTLNGLLLSLRRLELTAGRD
                     FEVIAVSFDPHEGADLARAKRDAYLERYGRTYSPCPSCTRGWHFLTGDAPQITALLGA
                     LGFRYFWDQDLRQYAHAAAIVVVTPRGRIAQYFNGVSYPPAQLRATLLEAAAEHSGSL
                     AERLWLLCFHYESLAGRYSALIASLLRLLGLAMVLALIVLITRLIRNSP"
     gene            complement(2170..2535)
                     /locus_tag="VI195_05380"
     CDS             complement(2170..2535)
                     /locus_tag="VI195_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823850.1"
                     /translation="MSGKRKRAQQWDVNVHRVIAAAGVVVAAIVLALAVSRALLAGLG
                     VGIDDPSRAPRELPPAPREQVHPIADYQHYRAAEQAKLEGYAWVDRKSAIVQIPIERA
                     MALVARDKTAARPADRSHR"
     gene            complement(2532..>3350)
                     /locus_tag="VI195_05385"
     CDS             complement(2532..>3350)
                     /locus_tag="VI195_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823851.1"
                     /translation="AMDKTWYLSRGFFELRAALILVAWIVLALLLRRGQQLTRVSALG
                     LIVYVLSMTVAAVDWIGSLEPRWSSTALGLVVVTGQGLAAFAFATACATLSAIRAARS
                     HADERPPLLSAERCGDLANLLLTFVMTWAYLAFVQFLITWAEDLPRETVWYLPRLAGG
                     GGVLAIALILLQFALPFTLLLFRALKRDPRVLAGIAALLLLSSWLNVTWLIAPSLARS
                     QPILRWTDVIATVGVGGLWAYAVLRDLAFRPAAIDTLPPSQAHRSAEDGAAGHT"
BASE COUNT          629 a         1074 c         1168 g          479 t
ORIGIN      
        1 gtcgacgccg ccgagcgcga gcgacgccag cacgatgatc ccgcccgcca tgtagatgta
       61 ccagctcgcg aggttcaggc gcgggaaggc gagatcgtgc gcgccgatca tcagcggcac
      121 cagaaaattt ccgagcgtcg cgggaatgga cggaatgaga aaaaaccaca ccatgatcac
      181 accgtgggcg gtgaacagct tgttgtaggt ttcggccgtc accagcgtgc cgtgcggcga
      241 tagcagctcg aggcgcatga gcgtcgccgc cgcgccgccg atgaagaaga aggcggtgat
      301 ggacacggcg tacaggatcg caatgcgctt gtgatcgcgc gtcgtgagcc aggagcgcag
      361 actgtagccg gcgccgagat agctcatcgc ggaaacagcg gcattcatgg cgaatcttcc
      421 gcgcccggca ccgtggcctg cggggccagg gactggatgt actcgatcag ctcgagcagg
      481 tcctcgtcgg tggcccgccc ggcgaacgag ggcatctggt cggcgtagcc ggcggcgatc
      541 tctttgcccg gcaacaggat cgagtcgcgc agatagcgct cgtcgacgcg cacgaagctg
      601 ccgtcggcaa gcggaatggt gcggccgtac aggtggctca ggtccggtgc atgcacggcc
      661 gcgcccccga cgtggcagcc gctgcagccg aagttctgaa acttcgccgc accgcggctc
      721 gcgaggctcg attcgtgtga gccgctctcc agccagcgcg cataggcgtc cggctcgagg
      781 accacgatgc ggccgcccat gcgcgaatga tcggtgccgc agtactccgc gcacagcagg
      841 tggtacacac cgctgcgcgt ggcggtgaac cacagcaccg cgaagcggcc ggggaccaca
      901 tcgcgcttca ctcggaaggc gggcacatag aagctgtgaa tgacatcctg cgaagtcatg
      961 accagcttga ccggacggcc gcgcggcacg tgcagctcgt tgatctcgcg cgcaccggtg
     1021 ggatgctcga tcttccacat ccactgcctc gccaccacgt agatctgcaa cgggctgcgc
     1081 ggtgggcttt cgtagctgaa atacagccgc gccccccaaa agaaaaatcc gaggaagacg
     1141 aggaacggaa cggtgatcca cgttacctcg agccagcgcc ggtgctcgcc ggctgcgtcg
     1201 cgcatggtgc ggtctaccgc ggcgccgcgc cggtaacgga tgcagaaaat cacgagcagc
     1261 accatgatgg tggcagccac ggtcgcggtg attgccgtca cgctccacag cagcgcatcg
     1321 acgtgggtgg cgagctcgga ggcggctggc ggcatggcaa gcacggcgct cacggggaat
     1381 tacgtatcag ccgggtgatg agcacgatga gcgcgagcac catggcgagc ccgagcagcc
     1441 gcagcagcga tgcgatcagt gcgctatagc gtccggcgag cgactcgtag tgaaagcaca
     1501 gcagccacag ccgctcggcg agggagccgc tgtgctcggc ggcggcttcg agcagcgtcg
     1561 cgcgcagctg cgccggcgga taggagacac cgttgaaata ctgcgcgatg cgaccccgtg
     1621 gggtgaccac gacgatggcg gcggcgtgtg cgtattgcct gaggtcctga tcccagaagt
     1681 aacgaaagcc gagcgcgccg agcagcgccg tgatctgcgg cgcatcgccg gtgagaaagt
     1741 gccagccgcg cgtgcaggag gggcacggtg aatacgtgcg gccatagcgc tcgaggtagg
     1801 catcgcgctt ggcgcgggcg aggtcggctc cctcgtgcgg atcgaagctt acggcaatca
     1861 cctcgaagtc gcggcccgca gtcagctcga ggcggcgcag gctgagcagc aggccgttga
     1921 gggtcagcgt gcagaggttc ggacactcga agtacacggg cgcgatcacc gccggccgcg
     1981 cgccgaggag ctcaccgagc ttgaccggag tgccacgctc atccctcagc gtgacatcga
     2041 gcgggagctc gcgcccgagg tgctgatcga gccccacgcg ctgctggata tcggtcactg
     2101 tggtggcctg cgctggcaag accaggcagc agctcgcggc gaggccgata agcgcagcca
     2161 gccgcgccgt catcgatggc tccgatccgc agggcgcgca gcggtcttgt cacgtgccac
     2221 gagcgccatg gcgcgctcga tggggatctg cacgatggcg cttttccggt caacccacgc
     2281 gtagccctcg agcttggctt gttccgcggc ccgatagtgt tggtagtccg cgatcgggtg
     2341 cacctgctcg cgcggagcgg gtggcagctc gcgcggtgcc cgtgacggat cgtcgatgcc
     2401 gaccccgagt cccgccagca gcgcgcggct gacagcgagc gcgaggacga tggccgcgac
     2461 gacgacgccg gccgccgcga tcacacgatg aacgttgacg tcccattgct gcgcgcgctt
     2521 gcgcttgccg ctcatgtgtg gcctgccgcg ccgtcctcgg cgctgcggtg agcctggctg
     2581 ggcgggagag tgtcgatcgc tgcaggccga aatgccaggt cacgcagaac cgcgtatgcc
     2641 cacaacccgc cgacgcccac cgtcgcgatg acatccgtcc agcgaaggat cggttgcgag
     2701 cgggcgagcg agggtgcgat gagccatgtg acattcagcc agctcgacaa cagcaacagt
     2761 gctgcgatgc cggccagcac gcgcggatcg cgcttcagtg cgcggaacag cagcaatgtg
     2821 aacggcagag caaactgcag caggatgagt gcgatcgcga ggacaccccc gccgcccgcg
     2881 agtcgcggca gataccacac cgtctcgcgc ggtagatctt cggcccaggt gatcaggaac
     2941 tgtacgaaag ccaggtaggc ccaggtcatc acgaacgtga gcaatagatt ggcaagatcg
     3001 ccgcagcgct cggcgctgag tagcggaggg cgctcgtctg cgtgactgcg ggcggcccga
     3061 atcgcgctga gcgtcgcgca cgcggttgcg aaggcaaagg ccgcgagtcc ctgacccgtc
     3121 acaacgacca gaccgagtgc ggtcgatgac cagcgcggct cgagcgagcc gatccaatcc
     3181 actgcggcga ccgtcatgct gagtacatag acgatcaggc cgagcgcgct cacccgggtg
     3241 agctgctgcc cgcgccgcag caacagcgcg agcacgatcc aggccacgag gatgagcgcg
     3301 gcgcgcagct cgaagaatcc gcggctcagg taccaggtct tgtccatcgc
//
LOCUS       DATDMP010000312         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1271587, whole genome shotgun sequence.
ACCESSION   DATDMP010000312 DATDMP010000000
VERSION     DATDMP010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271587"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1014)
                     /locus_tag="VI195_05390"
     CDS             complement(112..1014)
                     /locus_tag="VI195_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6823852.1"
                     /translation="MTRRALMLREGKAPVWERLFTMLFLAGLVHGLIILGLTFNAAAS
                     DKDSTPGLEVLLVSDEVPEADQNPTATYLAQRTQMGSGNRKKPVKPHNRASALPAIHH
                     PGSTEGKSLAASADQSGNAEERVLTTTGWSTRVHYLADLGTTGGQLDRPLLLDAPQTA
                     QPGPEDDPGPAELRGPNRDELWITPDTRMAVLAPYLDAWRRKVERIGTLNFPTVARTT
                     GATASPLVAVAIDADGKIESAVIRRSSGDPELDQAALATLKLASPFDPFPPDLSARYH
                     VLHFAYEWQFSGGRSGGGALSTLP"
     gene            complement(1028..2047)
                     /gene="gshB"
                     /locus_tag="VI195_05395"
     CDS             complement(1028..2047)
                     /gene="gshB"
                     /locus_tag="VI195_05395"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY6823853.1"
                     /translation="MTLSKRLGVVMDPIGAISYAKDSTLAMLLAAQARGFELAYLEQR
                     DLLLRDGVALGRRRALTVRPDAGSWFTLGEPALAPLAELDCVLMRKDPPFDTEYIYTT
                     YILERAEAAGVLVVNRPQGLRDMNEKVYTATFPQCCAPTLVTRDMGEMAAFLREHGKV
                     VVKPLHTMGGRSIFVLSLGDPNARVVFETLTDYGERFAIAQRYLPDIVHTGDARVLLV
                     DGEPVPYALARMPSAEDHRGNLAAGARGVARPLNERDRWLAGQIGPTLAAKGMLFVGL
                     DVIGGFVTEINVTSPTGIRELDKQCGTSIGDQLMAVIERRLAARPHAPRAAAATPAAS
                     AGSAG"
     gene            2393..>2724
                     /locus_tag="VI195_05400"
     CDS             2393..>2724
                     /locus_tag="VI195_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6823854.1"
                     /translation="MKVLVIDDSKTIRRTAETLLAKEGCEVFTAIDGFDALAKIADHQ
                     PDIVFVDIMMPRLDGYQTCSLIKHNKVFRSIPVIMLSSKDGLFDRARGRIVGSEHYLT
                     KPFTKDELL"
BASE COUNT          441 a          907 c          925 g          451 t
ORIGIN      
        1 agactgccgc cctgggcatc ggcgaatcgt ggcggggccg aggtcgccga gccgctcacg
       61 gaagtgtgct tgccggccat ggctccagta tgggcatgcg agccttcact ctcaaggaag
      121 tgttgacaat gcgccgccgc ctgaacggcc gccgctgaac tgccattcgt aggcaaagtg
      181 caacacgtgg tagcgggccg agagatcggg cgggaacggg tcgaaggggc ttgccagctt
      241 cagcgtggcg agtgccgcct gatcgagctc cggatcgccg ctgctgcgcc ggatcactgc
      301 cgactcgatc ttgccgtcgg cgtcaatggc caccgccacg agcgggctgg cggtcgctcc
      361 ggtggtgcgc gccaccgtcg gaaagttgag cgtcccgatg cgctcgactt tgcgccgcca
      421 ggcatcgagg tagggggcca gcacggccat acgcgtatcg ggagtgatcc acagctcgtc
      481 gcggttcgga ccccgcagct ctgcaggccc cggatcatcc tctggaccgg gctgcgccgt
      541 ctgcggggca tcgagaagca gtggccggtc gagctggccg ccggtggtgc cgagatctgc
      601 aaggtagtgc acgcgggtgc tccagccggt agtggtgagc acgcgctcct cggcattgcc
      661 cgactgatcg gcgctcgctg ccagggattt gccctcggtg ctgcctggat ggtggatcgc
      721 cggcaatgcg gaggcacgat tgtgcggttt gacgggcttt ttccggttgc cggagcccat
      781 ctgggtacgc tgcgccaggt aggtcgcggt cggattctga tccgcctcgg ggacctcatc
      841 ggataccagc aacacctcga gtcccggcgt gctgtccttg tcgctggcgg cggcgttgaa
      901 ggtgagcccg aggatgatga gtccatggac cagccccgcg aggaacagca tggtgaagag
      961 ccgctcccat acgggcgcct tgccctcccg cagcatgagc gcccggcgag tcatccgccc
     1021 gctccagtca gcccgctgag ccggcggagg cggccggggt ggcggcggcg gcgcgcggcg
     1081 cgtgcggcct cgccgcgagg cgccgttcga tgacggccat cagctgatcc ccgatcgagg
     1141 tgccgcactg cttgtcgagc tcgcggatgc cggtcgggct cgtcacgttg atctcggtga
     1201 cgaagccacc gatcacatcg agcccgacga acagcatccc cttggccgcg agcgtggggc
     1261 cgatctggcc cgcgagccag cggtcgcgct cattcagcgg ccgggcgacg ccacgcgcac
     1321 cggcggcgag gttgccgcgg tgatcctcgg ccgagggcat gcgtgcgagt gcgtacggca
     1381 ccggctcgcc gtccaccagc agcacgcgcg catcgcccgt gtgcacgata tcgggcaggt
     1441 agcgctgcgc gatggcgaag cgctcgccgt agtcggtgag cgtctcgaac accacccgcg
     1501 cattcggatc gccgaggctc agcacgaaga tcgagcgccc gcccatggtg tgcaggggct
     1561 tcaccacgac cttgccgtgc tcgcgcagga aggccgccat ctcccccatg tcgcgcgtca
     1621 ccagggtcgg agcgcagcac tgcgggaagg tggccgtgta caccttctcg ttcatgtcgc
     1681 gcagcccctg cggacgattc accaccagca cacctgccgc ttccgcacgc tcgaggatat
     1741 aggtggtgta gatgtattcg gtatcgaagg gcgggtcctt gcgcatcagc acacaatcga
     1801 gctcggcgag cggggcgagc gcaggctcgc ccagcgtgaa ccagctgccg gcatcgggcc
     1861 gcaccgtgag cgcgcgccgc ctgccgagcg ctacaccatc gcgcagcagc agatcgcgct
     1921 gctcgaggta ggcgagctcg aagccgcgcg cctgcgccgc gagcagcatc gccagtgtcg
     1981 aatccttcgc gtagctgatg gcgccgatcg gatccatcac cacgcccagc cgtttcgaga
     2041 gggtcatcgg gggcgattat gctcgtcggc ggcacgaata tgtctgccgc cggcacgggc
     2101 gtcgcgcgcc gctgcggtga ccttcgaaaa acgtgacgtg catggcatag cagggggtgt
     2161 accgatatgt taaaaccgcc ctgcgtttgc cggtggggca gggggttcgt gagactacgc
     2221 agaccgtgtg gaccgcgcgc cgcccttggt gcgctttggc gttgttccgc gaagcccatc
     2281 cgatccgccg cgcacacgcg tggcatgccg cccggtatgg ctgcatacgc cgctgaggct
     2341 tgaaggaagg agtcccctga ccgtgaacgg cagcaacgcg aagctccagg gcctgaaggt
     2401 actggtcatc gacgacagca agaccatccg ccgcaccgcc gagacgctgc tcgccaagga
     2461 gggctgtgaa gtcttcaccg cgatcgatgg cttcgacgca ctcgccaaga tcgccgacca
     2521 ccagcccgat atcgtattcg tcgacatcat gatgccgcgc ctcgatggct accagacctg
     2581 ctcactcatc aagcacaaca aggtgttccg ctccattccg gtgatcatgc tctcttccaa
     2641 ggacggactg ttcgaccggg cgcgtggccg catcgtcggc tccgagcact atctcaccaa
     2701 gcccttcacc aaggacgagc tgct
//
LOCUS       DATDMP010000313         3861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1492667, whole genome shotgun sequence.
ACCESSION   DATDMP010000313 DATDMP010000000
VERSION     DATDMP010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492667"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..964
                     /gene="cysQ"
                     /locus_tag="VI195_05405"
     CDS             161..964
                     /gene="cysQ"
                     /locus_tag="VI195_05405"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY6823855.1"
                     /translation="MSADPGRLLERSTSIARAAGREILEVYGAGALASTAKADDSPLT
                     AADLRSHRLIVEQLGELTPELPVLSEESAHVPFALRSQWRRYWLVDPLDGTREFLSRN
                     GEFTVNIALIEDHLPVLGVVHVPVTDTTYCGVPAAGAWRQQAAEPAVAIRVAAHSATP
                     LRIVGSRSHRGDSLDAFLGRVGPHALLAIGSSLKICLVAEGAADVYPRLGPTSEWDIA
                     AAHAVLLAAGGTLRALDGAPLAYNTREALLNPFFVAFGPDDRDWLALLR"
     gene            1020..2222
                     /locus_tag="VI195_05410"
     CDS             1020..2222
                     /locus_tag="VI195_05410"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY6823856.1"
                     /translation="MREAVIVSTARTPIGKAHRGAFNDTDPAVLSGHAVREAMRRAGV
                     DGAEVEDLVLGCAIQQGAQGYNIGRLTVASAGLPESVAGMTIDRKCASGLMAIATAAR
                     QVVEDGMDVVIAGGVESISLTQNKHFNSFRGESEAVLARSPHMYMPMIETAEVVARRY
                     RISRESQDRYALQSQQRVAAAYQAGRFDAEIVPLASRKLILDKATGAAQPQEVLLQRD
                     EGYRPDSTLEGLAKLEPVWSRGKVIAHGEFITAGNASQLSDGASAVVIMEAGLAERRG
                     LEPLGACRGIAVAGCAPDEMGIGPVFAVPKLLARHQLQVDDIGLWELNEAFAVQTVYC
                     RDRLGIDNDKLNVDGGAIAIGHPYGMSGARLVGHALLEGRRRRVRHAVVTMCVAGGMG
                     AAGLFEVY"
     gene            2271..2807
                     /locus_tag="VI195_05415"
     CDS             2271..2807
                     /locus_tag="VI195_05415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY6823857.1"
                     /translation="MSTAFSQRELRDAFGLFATGVTVVTAVRPDGTPVGVTANSFSSV
                     SLEPPLLLWCLAGASSAAPAFTQGAPFAVHILAHHQLDLALNFARRTHEKFEVDRHWR
                     NRSQPPHIDDALCRFDSRVHAVHAGGDHLIIVGEVLGIACGPGTPLAFHAGRFGNFSA
                     DRGIGKVDIWEGLEDHWY"
     gene            2841..>3861
                     /locus_tag="VI195_05420"
     CDS             2841..>3861
                     /locus_tag="VI195_05420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6823858.1"
                     /translation="MQFAHSEEQQQIRASARTLLSARAGPAQLRATLRAPGGYDAELW
                     RTLGELGWIGLAIPQQYGGAGLGWVELCLLQEEQGRRLVASPFFATSALAAPLILSCG
                     HETQRRALLPRIAAGELRIACALAGSEGRPPPDGIGVSLASRGQGFTLSGVSDFVIHG
                     DAADLLLVLARTAPSPGTDGISVAAIPAATAGITIHPHVMLDLTRPMSRVEFHEVRVQ
                     GEQLLGEPGAADSAVAEALDRARIALASEALGGAEQVLEMTTAYLKERTQFGRPIGSF
                     QALKHRLADMMIEVEAAKSAAWYAACVADERREELAEAAAIAKSYCCDAFFDCAANAI
                     QLHGGI"
BASE COUNT          573 a         1319 c         1350 g          619 t
ORIGIN      
        1 aggcgcgccg cggcgagctg aagaatttca ccggcatcga ttccccctac gagcggccag
       61 agaaccccga gattcgcatc gacaccaccg cgctcaatgc cgatgaggct gcggcgcgcg
      121 tgctcgccca tctcgagtcg cgcaaggtga tcggcagcgc atgagtgccg accccggccg
      181 cctgctcgag cgcagcacat cgattgcgcg cgccgcgggc cgcgagatac tcgaggtgta
      241 tggcgccggt gcgctcgcat cgacggccaa ggcggatgac tcaccgctca ccgctgccga
      301 tctgcgttcg caccggctca tcgtcgagca gctcggggag ctcacgccgg agctgccggt
      361 gctgtcggag gagtctgcgc acgtcccctt cgcgctgcgc agccagtggc ggcgttactg
      421 gctggtcgat ccgctcgatg gcacacggga gttcctctcg cgcaacggcg agttcaccgt
      481 caacatcgcg ctcatcgaag accacctgcc ggtgctcggt gtcgtgcacg tgccggtgac
      541 cgataccacc tattgcgggg tgccggctgc gggcgcctgg cggcagcagg ccgccgagcc
      601 ggcggtggcg atccgcgtgg cggcgcactc ggcgactccg ctgcgcatcg tcggcagtcg
      661 ctcgcatcgc ggcgattcgc tcgatgcctt tctcggccgg gtcggtcccc atgcgctgct
      721 cgcgatcggc agctcgctga aaatctgcct ggtagcggaa ggtgcggccg atgtgtatcc
      781 gcgcctcggc ccaacgagcg aatgggacat cgcggccgcg catgcggtgc tgctggccgc
      841 cggcggcacg ctgcgcgcgc tggatggtgc gcccctcgcc tataacacgc gcgaggcact
      901 cctcaacccc ttcttcgtgg ccttcggccc cgacgaccgc gactggctcg cgctgctgcg
      961 ctgagcgcgc ggccgtgcca tgctcagcgg ccgcagcgtc actccgccgg gagcccacca
     1021 tgcgtgaagc cgtgatcgtc tccactgcgc gcaccccgat cggcaaggcg catcgcggcg
     1081 ccttcaacga caccgatccc gcggtgctct cgggccacgc ggtgcgcgag gccatgcgtc
     1141 gcgcgggcgt cgatggcgcc gaggtcgagg atttggtgct cggctgcgcc attcagcagg
     1201 gcgcacaggg ctacaacatc ggccggctca cggtcgcgag cgcaggcctt ccggagagcg
     1261 ttgccggcat gaccatcgat cgcaagtgcg cctcgggtct catggcgatc gccaccgcgg
     1321 cacgccaggt ggtcgaggac ggcatggatg tggtgatcgc cggcggtgtg gagtcgatca
     1381 gtctcacgca gaacaagcat ttcaacagct tccggggcga gtccgaagcg gtgctggcgc
     1441 gctcgccgca catgtacatg cccatgatcg agaccgccga ggtggtagcg cgacgttatc
     1501 gcatcagccg cgaaagccag gaccgttatg cgctgcagag tcagcagcgc gtcgccgccg
     1561 cttaccaggc gggccgcttc gatgcggaaa tcgtgccgct cgcgagccgc aagctgatcc
     1621 tcgacaaggc gaccggcgcg gcacagccgc aggaggtgct gctgcagcgg gacgagggtt
     1681 atcgccctga cagcacgctc gagggtctcg cgaaactcga gccggtgtgg tcgcgaggca
     1741 aggtgatcgc acacggcgag ttcatcaccg cgggcaacgc ctcacagctc tccgatggcg
     1801 ccagcgcggt ggtgatcatg gaggcgggcc tcgccgagcg gcgcgggctc gagccgctcg
     1861 gtgcctgtcg cggcatcgcg gtcgccggct gcgcgcccga tgagatgggc atcggcccgg
     1921 tgttcgcggt gccgaagctg ctcgcccgcc atcagctgca ggtcgatgac atcggcctgt
     1981 gggagctgaa cgaggctttt gcggtgcaga ccgtgtactg ccgcgaccgc ctcgggatcg
     2041 acaacgacaa gctcaatgtc gatggcggtg cgattgccat cggccacccg tacgggatga
     2101 gtggggcgcg cctcgtcggc catgcgctcc tcgaaggccg gcggcgccgc gtgcgtcatg
     2161 cggtggtcac catgtgcgtc gccggcggca tgggcgcggc gggcctgttc gaggtgtact
     2221 gagccgggcg gcggcttgaa aatatcaagc cagcccgtag gatcgtcagc ttgagcactg
     2281 ctttttcaca gcgcgagctg cgcgatgcct tcggcctgtt tgctacgggc gtgaccgtgg
     2341 taacggcggt gcgaccggat ggaacgccgg tcggtgtaac ggccaattcc ttcagctcag
     2401 tgtcgctcga gccgccgctg ctgctgtggt gtctcgccgg cgccagcagc gcggcaccgg
     2461 cctttacgca gggtgcgccc tttgcggtac acatccttgc gcatcaccag ctcgatctcg
     2521 cgctcaactt cgcgcggcgc acgcacgaga aattcgaagt cgaccgtcac tggcgcaacc
     2581 ggtcgcagcc gccgcacatc gacgatgccc tgtgccgctt cgacagcagg gtccacgcgg
     2641 tgcacgcggg cggcgatcat ctcatcatcg tcggcgaggt gctcggtatc gcctgcggcc
     2701 ccgggacgcc gctcgcgttt cacgccggcc gcttcggcaa cttcagtgcc gatcgtggca
     2761 tcggcaaggt cgacatctgg gagggcctgg aggatcactg gtactgagca cggtgccgcg
     2821 gtgcctcggg gagtacgggc gtgcagtttg cccatagcga ggaacagcaa cagatccgcg
     2881 cgagcgcgcg cactctcctg agtgcacgcg caggtcccgc tcagctgcgt gcgacgctgc
     2941 gcgcgccggg cggctacgac gcggagctct ggcgcaccct cggggagctc ggctggatcg
     3001 gactggcgat cccacagcag tacggtggtg cgggtctcgg gtgggtggag ctctgtctgc
     3061 tgcaggaaga gcaggggcgg cgcctcgtcg cctcgccgtt cttcgccacg agtgcgctcg
     3121 ccgcgccgct catcctctcg tgtggccacg aaacgcagcg ccgggcactg ctgccacgca
     3181 tcgcagccgg cgagctgcgc atcgcctgcg cgcttgccgg gagtgagggg cgtccgccgc
     3241 ccgatggcat cggcgtctcg ctcgcaagcc gcgggcaggg attcacgctg agcggtgtca
     3301 gcgatttcgt catccatggc gacgcggcgg atctgctgct ggtgctcgcg cgcaccgcgc
     3361 cctcgccggg aaccgacggc atcagcgtcg cagcgatccc cgcggcaacc gccggtatca
     3421 ccatccatcc gcacgtgatg ctcgatctga cgcgaccgat gtcgcgggtc gagtttcacg
     3481 aggtccgcgt gcagggcgag cagctgttgg gcgagcccgg cgcggcagac agcgccgtcg
     3541 cggaggcgct cgatcgcgcg cgcatcgcgc tcgcctccga ggcgctcggc ggagccgagc
     3601 aggtgctcga gatgacgacc gcgtatctca aggagcgcac gcagttcggc cggccgatcg
     3661 gcagcttcca ggcgctcaag caccggctcg cggacatgat gatcgaggtc gaagccgcca
     3721 agtccgccgc ctggtacgcc gcctgtgtcg cggacgagcg gcgcgaggag ctcgcggaag
     3781 ccgccgccat agccaagagc tactgctgcg acgccttctt cgactgcgct gccaacgcca
     3841 ttcaactgca cggcggcatc g
//
LOCUS       DATDMP010000314         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1048599, whole genome shotgun sequence.
ACCESSION   DATDMP010000314 DATDMP010000000
VERSION     DATDMP010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048599"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..2578
                     /gene="dnaE"
                     /locus_tag="VI195_05425"
     CDS             32..2578
                     /gene="dnaE"
                     /locus_tag="VI195_05425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY6823859.1"
                     /translation="MPPRISLPEGQTPSSYLRALCDAAVPTLYPAEERSAASRQLDHE
                     LAVIGQLGLEEFFLTVWDIMREAHARRIRCSGRGSAANSITAYLLGITGVDPLRHHLL
                     FERFLNVERATMPDIDVDVQSGRRQELIEYVETRYSGHEAMVANVVQYRLRLAVRDVA
                     KALGFPLSVVNRLTRILPSYGSCSEIGRYGAELAQVLARKPDTGQDATAPTAPATRAQ
                     WTHRLHLLLELVPHLEHVPRHLSLHNGGLLLTRWPLDECLPLRRSANGVVAIEIDKDD
                     VEVIGMIKFDLLGLRTFDAIEHCLDLVEVTDGVRPDVDALPLDPPDEPTMALIRAGRT
                     LAVFQLESPAQWNLLSKTRPERFDDLVIQTSICRPGPIQGGMVHPYIARRAGRAPVRY
                     LHPTLEPILRDTLGVILFQEQILQIAHVVAGLTYGEADGFRKAISHYRTTSEMEGMRT
                     VFLQGAVRKGIPLPIATRIFAEIACFSGYGFCRSHAASFAKAIYQTAYLKAHFPAHYL
                     AGFLSAQPAGFFPPATVLHEARLLGIPLLPVDIATSREVYTVERLDGEVEEEGGRPLA
                     IRVALTQVNGVGEELARAIVAERDRGGPFRSLASFCQRMQSGQTHVPLRHDAVAALIR
                     AGAFDGLSIPRRRLLWLLAERWQNWQVPQPRARVRQGRSQRRVATASASPAAIQVPLP
                     LIWPDEQPADAPRVLPLTLREEVEWDLAAQGLSVRPHPLTFRRKELSGVGVVPIAALA
                     GRCAGERALVAGLVISAQRPPTAKGTAFVTLEDETGRVEAVVQSHLAESIRRLLAESR
                     LLAISGRIERSGDHVTLLARALRVVPAAIQPRLSPIQASVPSDRVRGVIA"
BASE COUNT          447 a          960 c          954 g          493 t
ORIGIN      
        1 ctgcgacgtc acgctgctcg ccgagcgctg cattccgccg cgcatctctc tgcccgaagg
       61 gcagacgcct tcatcgtact tgcgcgcgct ctgtgacgca gccgtcccca cgctctaccc
      121 cgccgaggaa cgctccgccg cgtcgcggca actcgatcac gagctggcgg tcatcgggca
      181 acttggactg gaggaattct tcctcacggt gtgggacatc atgcgtgagg cccacgcccg
      241 gcgcattcgc tgctctggcc ggggcagcgc cgccaacagc atcaccgcct acctcttggg
      301 catcacgggg gtggatccgc tgcggcatca cctgctattt gagcgctttc tgaacgtcga
      361 gcgcgcgacc atgccggata tcgatgtgga tgtgcaatcg ggtcggcggc aggagctcat
      421 cgagtatgtg gaaacgcggt actccgggca cgaggcgatg gtcgccaacg tcgtgcagta
      481 ccgactgcgc ctcgcggtgc gcgatgtggc gaaggccctc ggcttcccgc tgtcggtcgt
      541 gaaccgtctg acgcgcatcc tgccgtctta cggttcgtgc agtgagatcg gccgctatgg
      601 cgccgagctg gcccaggtac tggcgcgcaa gccggacact ggccaggacg ctactgcacc
      661 tacggcaccg gcgacgcggg cgcagtggac gcaccgcctg cacctgctgc tcgagctcgt
      721 cccgcacctg gagcacgtcc cccggcacct gagtctgcat aacggcgggc tgctgctgac
      781 gcgctggccc ttagacgaat gcctgccgct gcgccgctcg gcgaacgggg tggtcgccat
      841 cgagatcgat aaagatgacg tcgaagtcat cgggatgatc aaattcgacc tgctcggcct
      901 gcggaccttt gacgccatcg agcactgcct cgacctggta gaagtcactg acggcgtgcg
      961 gcccgacgtc gatgccctcc cgctcgaccc gcccgatgag cccacgatgg cgctcattcg
     1021 tgcggggcgg acgctggccg tctttcagtt ggagagtccg gcgcaatgga atctgctctc
     1081 caaaacacgg cccgagcgct tcgacgacct tgtcattcag acctcgatct gccggcctgg
     1141 gccgatccag ggcggcatgg tccatccgta tatcgcccgc cgcgcggggc gtgcccccgt
     1201 gcgctatctg cacccgaccc ttgagccgat cctgcgcgat accctcggcg tcatcctgtt
     1261 tcaagaacag atcctccaga tcgcacacgt ggttgccggc ctcacctatg gcgaggccga
     1321 tgggttccgc aaggccatct cgcactaccg caccacaagc gagatggagg ggatgcgtac
     1381 ggtcttctta cagggtgccg tccgcaaagg catcccgctg cctatcgcca cgcggatttt
     1441 cgccgagatc gcctgcttct ccggctacgg gttctgccgc agccatgcgg caagtttcgc
     1501 caaggccatc taccagaccg cctacctcaa ggcgcatttc ccggcccact atctggcagg
     1561 cttcctgtcc gctcagcccg ccgggttctt cccgccggcc accgtcctcc acgaggcgcg
     1621 actgctcggc atcccgctgc tgccggtcga catcgcgacc agccgcgagg tgtacacagt
     1681 cgagcggctc gatggggaag tggaggagga aggagggcga ccgctcgcga tccgcgtcgc
     1741 cttgacgcaa gtgaacggcg tcggggagga gctggcgcgg gcgatcgtcg ccgagcgtga
     1801 ccgcggcggc ccgttccggt cactcgccag cttctgtcag cggatgcaat ccgggcaaac
     1861 gcacgtaccg ctgcgacacg acgccgtcgc cgctctcatc cgcgccgggg cttttgatgg
     1921 cctgtccatc ccgcgccggc ggctgctctg gctcctcgcc gagcgctggc agaattggca
     1981 ggtgccgcag ccacgggcgc gagtgaggca gggtcggtcg caacgccgag tggcgacagc
     2041 atcggcatcc ccagccgcca tccaggtgcc cctgccgctg atctggcctg atgagcagcc
     2101 ggctgacgcg ccgcgtgtcc tgccgctgac attgcgtgaa gaggtcgagt gggatctggc
     2161 ggcgcagggg ctctccgtca ggccgcatcc gctcaccttc cgtcgcaagg agctgagcgg
     2221 ggtgggcgtc gtgcccatcg ccgcgctggc aggacgatgt gccggagagc gggcgctggt
     2281 cgcagggctg gtgatctccg cccagcgccc cccgacagcg aaaggaacgg cgtttgtcac
     2341 actggaggat gagaccgggc gcgtggaggc cgtggtgcag tcgcacctgg ccgagtccat
     2401 tcgccgttta ctggccgaat cgcggctgct cgccatttcc ggccggatcg agcggtccgg
     2461 cgaccacgtg acgctgctgg cgcgggcgct acgggtggtg ccggcggcga ttcaaccgcg
     2521 cctctctccc atccaggcga gcgttccatc cgacagggtg cgcggtgtga ttgcttagtc
     2581 ggcgggctgg tgagtcttta ctggtgcctt gtcacgggtg atttgacaga acgaggcatg
     2641 gcgactatcc tgcgccagac gggaggggag gaggtcgcga tgcgtaaggc gtatgtggta
     2701 cggctgacgg acgacgagcg acgtgggctt gagcgggtgg tgacggcggg acaggccacg
     2761 gcccgcacac tgacccacgc atacatccta ctcaaagcgg acgcgggagc agcagaacca
     2821 gggtggcggg atgcgcagat caccgcggcg ttcg
//
LOCUS       DATDMP010000315         2529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_160279, whole genome shotgun sequence.
ACCESSION   DATDMP010000315 DATDMP010000000
VERSION     DATDMP010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160279"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VI195_05430"
     CDS             complement(<1..1098)
                     /locus_tag="VI195_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6823860.1"
                     /translation="MTVSAGNEAAPRLNPSEPFIRRPVATTLLSLGVALAGAMTYTLL
                     PVAPLPQVDSPTISVSASMAGASPDVMAATVATPLERHLGQIADVTEMTSSSSTGASH
                     ITLQFGLDRDIDGAARDVEAAINAARADLPTALRSNPTYHKVNPADAPVLILALTSHT
                     LTPGQLYDAATTILQQKLSQVRGVGQVNIGGSSLPAVRVELNPTALFKYGIGLEDVRA
                     ALAAANANTPKGSMDVGAEHYQIYTNDQATHAAEYRNLVIAYRNGAAVRLADVAEVLD
                     SVENVRNLGLANGSPAVLVILYKAPDANIIETVDAVKRLLPALQASIPTDIAIKVAMD
                     RTTTIRSSLRAVESTLAMAVALVILVVFLFLR"
     gene            complement(1095..>2529)
                     /locus_tag="VI195_05435"
     CDS             complement(1095..>2529)
                     /locus_tag="VI195_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6823861.1"
                     /translation="SYADMARRQATLAAAILKDADVVSLSSFIGVDGSNVTLNSGRML
                     INLKPVDERSASASDVIRRLQRETAEVSGISLYMQPVQDLTIDTSVSRTQYQFVLEDA
                     NPEEFNTWVPRLVTRLQQLPQIEDVASDQQNQGLAAYLTIDRDTAGRFGITPATVDNA
                     LYDAFGQRIVSTIFTQSNQYRVILQADSAGLASLDALGTLYLPSSTAVGGQVPLSALV
                     KVSTRLSPLAIDHLGQFPATTVSFNLAPGAALGDAVDAIRKVEADIHMPPSVITTFQG
                     AALAFQATLANEVFLILAAIITVYIVLGVLYESFIHPVTILSTLPSAGVGALLALLLA
                     RMDLGVIAIIGIILLIGIVKKNAIMMIDFALVAEREHGMGPRQAIHHASCLRFRPILM
                     TTMAALLGALPLMLASGTGAELRRPLGVTIVGGLILSQLLTLFTTPVIYLYFDRLERR
                     VARWGSPGSGSAGAVSPAGIRRPEDTL"
BASE COUNT          444 a          767 c          911 g          407 t
ORIGIN      
        1 gcgcaggaac aggaacacca ccagaatcac cagcgccacc gccatggcga gtgtgctctc
       61 gaccgcgcgc agcgaagagc gaatggtggt ggtgcggtcc atcgccacct tgatggcgat
      121 gtcggtggga atcgaggcct gcagcgcggg cagcaaccgc ttgaccgcat cgaccgtctc
      181 gatgatgttg gcatcgggtg ccttgtagag aatgaccagc accgcggggc tgccgttggc
      241 gaggccgagg ttgcgcacgt tctcgaccga atcgagcacc tcggcgacgt ctgcgagccg
      301 caccgccgcg ccgttacggt aggcgatcac gagattgcgg tactccgcgg cgtgcgtcgc
      361 ctggtcgttg gtgtagatct gatagtgctc cgcgccgacg tccatcgagc ccttcggggt
      421 gttggcattg gcggcggcga gtgccgcgcg cacgtcctcg agcccgatgc cgtacttgaa
      481 gagcgccgtg gggttgagct ccacgcgcac agcggggagc gaggagccgc cgatatttac
      541 ctggccgacc ccacgcacct gagagagctt ctgctgcagt atggtggtgg cggcatcgta
      601 gagctggcct ggcgtgagcg tatgcgaggt cagggcaagg atgaggaccg gtgcgtccgc
      661 cggattcacc ttgtgatagg tcggattgct gcgcagcgcc gtaggcagat ccgcacgcgc
      721 ggcgttgatc gctgcctcga catcgcgcgc ggcgccgtcg atgtcgcggt cgagaccgaa
      781 ctgcagcgta atgtgcgaag cgccggtgga gctcgatgag gtcatctcgg tgacatcggc
      841 aatctgcccg agatgacgct cgagcggcgt cgccaccgtg gctgccatca catcggggct
      901 cgccccggcc atcgaggcgc tcaccgagat cgtaggcgag tccacctggg gtagcggggc
      961 aaccggcagc aaggtgtagg tcatcgcccc ggcgagtgca acgccgaggc tgaggagcgt
     1021 ggtagccacc gggcgccgaa tgaagggctc ggaggggttc agccgcgggg ccgcctcatt
     1081 gcccgcgctg acggtcacag cgtgtcctcc ggtcgccgga tgccggccgg actcaccgcc
     1141 ccggcgctcc cgctcccggg actgccccag cgggcgactc ggcgctcgag gcggtcgaag
     1201 tagagataga tcaccggtgt ggtgaacagc gtcagcagct gcgagaggat cagtccgccg
     1261 acgatggtga caccgagcgg acggcgcaac tcagcgccgg taccggaggc gagcatgagc
     1321 ggcagagcgc caagcagcgc cgccatggtg gtcatgagga tcggccggaa gcgcaggcac
     1381 gaggcgtgat gtatcgcctg gcgtggcccc atgccgtgtt cgcgctcggc gaccagcgcg
     1441 aagtcgatca tcatgatggc gttctttttc acgatgccga tcaacaggat gatgccgatg
     1501 atggcgatga cgccgagatc catgcgcgcg agcagcagcg ccagcagcgc gccgacaccc
     1561 gccgagggca gcgtggagag aatggtgacc gggtggatga agctctcgta gagcacgccg
     1621 agcacgatgt agacggtgat tatcgcggcc aggataagga acacctcatt ggcgagggtc
     1681 gcctgaaagg cgagcgcggc gccctggaag gtggtgatca cgctcggcgg catgtggatg
     1741 tcggcttcca ccttgcggat cgcatccacg gcatcaccga gcgcagcgcc cggcgccaga
     1801 ttgaacgaca cggtggtcgc gggaaactgc ccgaggtggt cgatggcaag cggcgagagc
     1861 cgcgtcgaca ccttcaccag cgccgagagc ggcacctgcc caccgaccgc ggtcgaggat
     1921 ggcaggtaga gggtgccaag cgcgtccagc gaggcgagac ctgcactgtc ggcctgcagg
     1981 atcacgcggt actggttcga ttgcgtgaag atagtcgaga cgatgcgctg gccgaaggcg
     2041 tcgtagagcg cgttgtcgac cgtggcgggc gtgatgccga agcggccggc cgtgtcgcgg
     2101 tcgatggtga gataggccgc aagcccctgg ttctgctgat cgctcgccac atcctcgatc
     2161 tgcggcagct gctgcagccg tgttaccagg cgcggtaccc aggtgttgaa ctcctccggg
     2221 ttcgcgtctt cgagcacgaa ctgatactgc gtgcggctca cgctggtatc gatggtgaga
     2281 tcctgcaccg gctgcatgta gagcgagatg ccgctgacct ccgcagtctc gcgctgcagc
     2341 cggcggatca catcgctcgc cgaggcggag cgctcatcga ccggcttgag gttgatcaac
     2401 atgcggccgc tgttgagggt gacattcgag ccgtcgacgc cgatgaacga ggacaggctg
     2461 acgacatcgg catccttgag gatcgcggcc gcgagggtcg cctggcggcg tgccatgtcg
     2521 gcgtaggaa
//
LOCUS       DATDMP010000316         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_938353, whole genome shotgun sequence.
ACCESSION   DATDMP010000316 DATDMP010000000
VERSION     DATDMP010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938353"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /gene="parC"
                     /locus_tag="VI195_05440"
     CDS             <1..1668
                     /gene="parC"
                     /locus_tag="VI195_05440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY6823862.1"
                     /translation="HLLEDPEATTAALMKHVKGPDLPTGAEIVSPRTDLKEFYDKGVG
                     TFKARATWEIEEGNVVITAFPYQVSPTRVQEQIAEQMRAKKLPMVDDLRDESDHENPI
                     RLVIVPRSNRVDLTEVMNHLFATTDLERNYRVNLNIIALDGRPRVMSLREILGEWLEF
                     RTTTVTRRLQHRLEKVTKRLHILDGLMVVYLNLDEVIRIIRREDEPKPVLMKRFKLSD
                     EQAEEILNTRLRHLAKLEEMRIREEQKALAAEREELEALLKSKAKLRKLIGSEIRADA
                     DKYGDERRTKIIEREAAQAIDETSLIPNEPVTVVLSTGGWVRSAKGHDIEPGALSYKS
                     GDAFQALARGRSLQPAVFIDSTGRTYTLPAHSLPSARGYGEPLSGRLDPPDGAKFAGV
                     MIGDPEDLWLLASDAGYGFTARLKDLITDRRAGKVVLNVPAHAQVLAPAPVPSADSLV
                     AVVSSEGKLLAFAVGEVPEMPRGKGNKLYDIPGKKAAARQELMTALAVVPPNATLFLW
                     SGEQQKTIDWRDLQDYRGQRAQRGAVLKRGWPRKITRMEARVPVTGS"
     gene            1927..>2530
                     /locus_tag="VI195_05445"
     CDS             1927..>2530
                     /locus_tag="VI195_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823863.1"
                     /translation="MNRPYSLRAPRPLARAAAFFVAAALAAASAARAAEPGLADLQRQ
                     LAQMQATLQQLADENRTLREHQAEVDRKLAQLSGDTPAAGAQAAPAAMAPAAAAGQPD
                     ASALPPTATAAPGTTGAGLFGTGLRVWGYGEVYYTHPIDEPAQTKMDLARAVFGIGYK
                     FSDRTEFNSEYELEHAVSSADDVGEFEVEQFYVDHRVSDAV"
BASE COUNT          461 a          857 c          834 g          378 t
ORIGIN      
        1 catctgctgg aggacccaga agcgaccacc gccgcgctca tgaagcatgt caagggcccg
       61 gacctgccga cgggtgccga gatcgtctcg ccgcgcacgg acctcaagga gttctacgac
      121 aagggtgtcg gcaccttcaa agcgcgcgcc acctgggaga tcgaggaggg caatgtcgtc
      181 atcacggcct ttccctatca ggtgtcgccg acgcgcgtgc aggagcagat cgccgagcag
      241 atgcgcgcca agaagctgcc aatggtcgat gacctgcgcg acgaatccga tcatgagaac
      301 ccgatccggc tcgtcatcgt gccgcgctcc aaccgcgtcg acctcaccga ggtgatgaat
      361 cacctgtttg cgaccactga cctggaacgc aactaccgcg tcaacctcaa catcatcgca
      421 ctcgatggcc gcccgcgcgt gatgagtctc agggagatcc tcggggagtg gctcgagttc
      481 cgcaccacga cggtcacgcg gcgcctgcag caccgtctcg agaaggtaac gaagcggctg
      541 cacatcctcg acggcctgat ggtcgtctac ctcaacctcg atgaggtgat ccgtatcatc
      601 cgccgggagg atgagccgaa gcccgtgctg atgaagcgct tcaagctctc ggacgagcag
      661 gccgaggaga tcctcaacac ccgactgcgc catctcgcca agctcgagga gatgaggatc
      721 cgcgaggagc agaaggccct cgccgccgag cgcgaggagc tcgaggcgct gctgaagagc
      781 aaggcaaagc tgaggaagct catcggcagc gagatccgcg ccgatgcgga taagtacggc
      841 gatgaacggc gcaccaagat catcgagcgc gaggcggcgc aggcgatcga cgagaccagc
      901 ctcatcccca acgagccggt gacggtggtg ctctcgaccg gcggctgggt gcgctcggcc
      961 aagggccacg acatcgagcc gggtgcgctg tcgtacaaga gcggcgatgc cttccaggcg
     1021 ctcgcccgcg gccgcagcct ccagccggca gtgttcatcg acagcaccgg ccgcacgtac
     1081 acgctgcccg ctcactcgct gccctccgcg cgcggctatg gcgagccgct ctccgggcgc
     1141 ctcgacccgc ccgatggcgc caagttcgcc ggtgtgatga tcggcgaccc cgaggatctg
     1201 tggctgctgg cgagcgatgc gggctatgga tttacggcgc gactgaagga tctcatcacc
     1261 gaccggcgtg ccggcaaggt ggtactcaac gtgccggcac atgcgcaggt gctcgcgccg
     1321 gcgccggtcc cctcggcgga ctcactcgtg gcagtggtct cgagcgaggg caagctcctc
     1381 gccttcgcgg taggggaggt ccccgagatg ccgcgcggca aaggcaacaa gctctacgac
     1441 atccccggca agaaggcggc ggctcgacag gagctcatga ccgcccttgc ggtcgttccg
     1501 ccgaacgcga ccctgtttct ctggtcgggt gaacagcaga agaccatcga ctggcgcgac
     1561 ctgcaggact atcgcggcca gcgtgcccag cgcggcgcgg tcctcaaacg tggctggccc
     1621 cgcaagatca cccgcatgga agccagggtc cccgttacgg gtagctgatc cggtccggct
     1681 tcgagggcca caggcttgcc tccatttgct aatgagaatg cttctcatta gtattcccct
     1741 ttacgatctt ggccgtttcg agggcgagtc atgttgaggg aggcacgcct ggcggccacg
     1801 gcgctcgccg gtgagtgtgc acgctgaccg accgcttcga tccccaacaa ccccacgacc
     1861 taaaacgtcc cgggcgcaat gcgcgccggg gccgagtcat tgctcgaacc gaatggaaga
     1921 ccccgtatga accgcccata ttccctgcga gccccgcgcc cactcgcacg cgccgccgcc
     1981 ttcttcgttg ccgcagcgct cgcggccgcc agcgcagcgc gcgcggccga gcccggcctc
     2041 gcggacctgc agcgccagct cgcgcagatg caggcgacgc tgcagcagct cgccgacgag
     2101 aaccgtacgc tgcgcgagca ccaggcggag gtggaccgta agctcgcgca gctgagcggc
     2161 gacacgccag cggcaggggc ccaggcggcg ccggccgcca tggcgcctgc agccgccgcg
     2221 ggccagccgg atgcctcggc gctgccgccc accgcgactg cggcgccggg caccacgggg
     2281 gcgggcctgt tcggcacggg cttgagagtg tggggctacg gggaggtgta ttacacgcat
     2341 ccgatcgatg agccggcgca gaccaagatg gatcttgccc gcgccgtgtt cggcatcggc
     2401 tacaagttca gtgaccgcac cgagttcaac tccgagtacg agctcgagca tgcggtgtcc
     2461 tcggccgatg acgtcggtga gttcgaagtg gagcagttct acgtcgatca ccgcgtgagc
     2521 gacgcggtga
//
LOCUS       DATDMP010000317        10491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2049311, whole genome shotgun sequence.
ACCESSION   DATDMP010000317 DATDMP010000000
VERSION     DATDMP010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049311"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VI195_05450"
     CDS             <1..368
                     /locus_tag="VI195_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY6823864.1"
                     /translation="MHIVSGVKGQLAPGRDAFDLFAATFPAGTLVGAPKVRAMEIIDE
                     LEPVSRGLYGGTVGYFGARGDMDHAITIRTLVFAGDEYSYQAGAGIVADSVPESEHAE
                     VLAKSAAMVRALELAEEGL"
     gene            365..979
                     /locus_tag="VI195_05455"
     CDS             365..979
                     /locus_tag="VI195_05455"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY6823865.1"
                     /translation="MKARLLLIDNYDSFTYNLVQAFLVLGAEVEVHRNDALTPAAARA
                     LAPSHLCISPGPGTPRDAGVSMDMIREFAGRIPVLGVCLGHQSIVAVFGGEIVRAGRL
                     MHGKTSLVRHDGGTLFAGLPQPCEVGRYHSLIAAPASLPAVLEVSARTDEGEIMAVRH
                     RSLVVEGVQFHPESILTPEGPKMLESFLRYRAGVRDGAAHVGAA"
     gene            963..2030
                     /gene="trpD"
                     /locus_tag="VI195_05460"
     CDS             963..2030
                     /gene="trpD"
                     /locus_tag="VI195_05460"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY6823866.1"
                     /translation="MSAPRELLQQLIERRDLAQAQAEALLAHLTDPQLPPAMAGALLA
                     ALSAKGLAATEVRGLAQAMRRLARRPDLPPDLRSIDIVGTGGDASGSFNISTGTALLT
                     AACGVPVVKHGNRSVSSRCGSADVLEALGLTVPLDAATARACFAATRFTYLFAPYYHP
                     AIGRVAAIRAALGVRTVFNILGPLVNPAEPPLHLVGAFSLEVAQLMAEAFQGLPIERA
                     FVVHGAAGWDEPTPMGPFTIFDVRPGSVAVSERSAADYGLPPCAAADLLGGDAAHNAQ
                     ALKAVLYGEDRGAHRDCLLLGTALALEVAGIVREAREGVARAAAAIDEGAARRLVELL
                     PRQSASVGSARGGLGSEGPAA"
     gene            2027..2857
                     /locus_tag="VI195_05465"
     CDS             2027..2857
                     /locus_tag="VI195_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823867.1"
                     /translation="MSSFLDEMAAASTARLRAARTHLSEAALLERASHAAAAPPLRLS
                     AEGFDLIAELKLRSPAAGALGAAAGSIGERAVAYAQAGAAAVSVLTEPSRFDGALSHL
                     SEAARALAPFGVPAMRKDFLIDPYQVLEARAAGAGGVLLILRMLSGETLGTLIECARA
                     QGLFVLLEAFDESDLERMRQVIAADAGRSTLLAGVNCRDLGTLEVVPQRLFELAPLMP
                     PQVPHVAESGIEVAADAARLAAAGYDLTLVGSALMRGADPGALARSLLAAGRSARGGS
                     "
     gene            2858..3445
                     /locus_tag="VI195_05470"
     CDS             2858..3445
                     /locus_tag="VI195_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY6823868.1"
                     /translation="MWIKICGMTTAAAVDAALAAEVDAIGFVFAPSVRELTPVAAAAL
                     ARPARGRVRCVAVTHHPTQTAVDEIVREFVPDLLQSDAEDFARLRLPAALETLAVFRE
                     GAAISVPPPARILYEGPASGRGQLIDWSAARALARLSELVLAGGLNEGNVAAAIAQVD
                     PFGVDVSSGVEERPGVKSPAAIVDFVAAVRAAGSI"
     gene            3462..4709
                     /gene="trpB"
                     /locus_tag="VI195_05475"
     CDS             3462..4709
                     /gene="trpB"
                     /locus_tag="VI195_05475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY6823869.1"
                     /translation="MSATVALPDAAQLLAAAVAGQFPDERGRFGPFGGRYVPETLIPA
                     LERLERGVRAHLHSQEFQAELSAQLTGWVGRPTALTCAPRLSASWGAQVWLKREDLAH
                     TGAHKINNAIGQALLAHRLGAKRIVAETGAGQHGAASAAACARLGLPCTVYMGSIDME
                     RQAPNVGRMRLLGARVVPVESGDRTLRAAIDEALRDWVSDPLDTYYLLGSVVGPHPYP
                     YLVRELQAVIGREARAQILAATGRLPDALIACVGGGSNSIGAFHAFVADTAVEIIGVE
                     AGGRGAALGDNAATVAHGRPGVLHGSYSLLLQNADGQIQETHSVSAGLDYPGVGPEHA
                     LLAASGRVRYEIASDAEALEGVRQLCEREGILPALESAHALAGAARWARRHPGGTVLV
                     CLSGRGDKDMSTLQQTLLAAERA"
     gene            4706..5476
                     /gene="trpA"
                     /locus_tag="VI195_05480"
     CDS             4706..5476
                     /gene="trpA"
                     /locus_tag="VI195_05480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY6823870.1"
                     /translation="MSPRERISSAIRAAAARGEPALVAYLTAGFPSRARFAEHLRTLA
                     DAADVIEVGVPFTDPMADGVTIQRASLAALAQGVSLAWILEEIAALGGLAAPLVLMSY
                     LNPLLAFGIARLAESAARAGVSGFIVPDLPLDESSALRHALDERELALVQMVTPVTGP
                     QRLAQLCAGSEGFVYAVTMTGTTGRNVAVPEQVLEYLDRVRAVAQLPVCAGFGIRSRE
                     QVERLRGHVDGVVVGSALVEVLERGEDAGAFLRGLRPA"
     gene            5816..7582
                     /locus_tag="VI195_05485"
     CDS             5816..7582
                     /locus_tag="VI195_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823871.1"
                     /translation="MRTQTWSCRAFLSAGLLLLSSSLAWGAPPHAEFLQCNEPGGILC
                     AEQRENPGGKEYYVGHDEPAVLFYSNTPGAGFNNVYRIRLPKDPPALPKKNGSGGTWN
                     FQLHPAFWFGMAMCDDQGNPNPGGSPSFANIPCTPDSDRNIFDSPDPTAPDYIGRHPG
                     TAFMEMQFYPPGWITSNSGAVDAESATKWTAALNIDSFQSNDNVVCGTEGPNCLGGVP
                     PGPSAIQANNAACSAITFDEYVNFSFIQTDGVPSASPSPYDLALNTTNAKTLRMDGGD
                     ELIVTIKDTHSGLMVTIQDLTSGNSGFMVASGANGFASVKWDPSGDNCLRATHELRHN
                     FHPAYATSSEHTRVPGAAHSYNIAFSDEIGHFEYCNGVAKEGGACTSASGSDKKADVD
                     DGAFGGCFDAGFAASLGLIQIGGCLDSDTDFDGVPYRNNTWPGSYSDPVQDALFHAQP
                     VIFSSPLLTDSGGHLRNFSRVAFETDMPRIEFATNPPCQRHLSNPADPNPGLGCVNPP
                     VGAAFYPFYSTRSDDGVCLWQEGGPFIPGTVNNFGASSQAQFGSIPLALFYPAVNIMP
                     QYIYEDFRNILTTNPCPAPTGD"
     gene            7727..8449
                     /locus_tag="VI195_05490"
     CDS             7727..8449
                     /locus_tag="VI195_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823872.1"
                     /translation="MQSLTIATRFRGPPESANGGYFAGLVATLAPRTVSVRLLKPPPL
                     NIGLAVSALEDGGLRVLRGEEPIGEAHPAVLTLTVPPPPDYLEAVEASRHYAGFRYHR
                     FPSCFVCGTQRARGDGLRIFAGPIAERDVVAAPWVPDASLDAGDGKVRAEFMSAALDC
                     PGYYAVAPDDRMMLLAEFTAHVDRRVHIGESCTLIGWCIGSDGRKHEAGTALFDGKGE
                     LCGRARALWIEPRATVSELSVK"
     gene            complement(8566..9939)
                     /locus_tag="VI195_05495"
     CDS             complement(8566..9939)
                     /locus_tag="VI195_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6823873.1"
                     /translation="MQRSLTEGSIPVGLFRFALPILFANILQSLNGSVNSIWVGRFLG
                     EAALTATSNANTVMFLLIGAAFGMALAATILIGQCIGANNLHETKRVVGTSATFFAGI
                     SVTMAIAGLLLCRPLLIAMSTPADSLPLAVAYMRVIFLALPWLYMYAFVMAVLRGAGD
                     SKTPFYFMLLSVGIDIVLNPVFIFGLGPIPRLGIAGSALATFVAQVVSLIALIRHLYR
                     RHHILCLHRQELHLLKPNGSLIGTLVRKGIPMSAQMLVLSLSGVLMITLVNRFGVDTT
                     AAFGAALQIWNYIQMPAFAIGMAVSAMTAQNVGAHKWHRVTTIARVGTLYSVLLTGSV
                     VLAIELLNSWVFGLFLPAASDALAIASHMNRIVTPSFIFFGISLTLFGVVRATGAVIA
                     PLIILTIALLLVRFPFAEAFLGRYQVDAVWWSFPVSSLLAALLAVLYYRYGDWRHAHM
                     ALPARSS"
     gene            complement(9940..>10491)
                     /locus_tag="VI195_05500"
     CDS             complement(9940..>10491)
                     /locus_tag="VI195_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823874.1"
                     /translation="VCEPIFDRPLKDISFGRILLRLFEISRRFNMQIQPQLMLLQKTL
                     LNVEGLGRDLYPDLDVWATATPILREWMRERTSVRALARELRARTPGLMEAARGLPLL
                     LGRLLERELAGVRAPAPAVDSLELRSALARAARRRDAVTLGAALLLGGLVWLAVAREP
                     AWVGWCLVVAGAAGVVYGLWRTN"
BASE COUNT         1703 a         3458 c         3554 g         1776 t
ORIGIN      
        1 tgatgcacat cgtcagcggc gtgaaaggcc agcttgcgcc tggccgcgac gccttcgatc
       61 tgttcgccgc cacctttccc gccggcacgc tggtgggcgc gccgaaggtg cgcgccatgg
      121 aaatcatcga cgagctcgag ccggtgagcc gcggcctgta cggtggcacc gtgggctact
      181 tcggcgcgcg cggcgacatg gatcacgcca tcaccatccg caccctggtg ttcgccggcg
      241 atgaatacag ctaccaggcg ggcgccggca tcgtcgcgga cagcgtcccc gagagcgagc
      301 acgcagaggt gctcgccaag agcgctgcca tggtgcgcgc gctcgagctg gcggaggaag
      361 gcctgtgaag gcgcgtctgc tgctcatcga caactacgac tccttcacct acaacctggt
      421 gcaggcattc ctcgtgctcg gcgccgaggt ggaagtgcac cgcaacgacg cactgactcc
      481 cgctgcggcg cgtgcgctcg cgccctcgca tctgtgcatc tcacccggac cgggtacgcc
      541 gcgcgatgcc ggggtatcga tggacatgat ccgcgagttc gccgggcgta ttccggtgct
      601 cggcgtctgt ctcggtcatc agtcgatcgt cgcggtgttc ggcggcgaga tcgtgcgtgc
      661 cggccggctc atgcacggca agacctcgct cgtgcggcat gacggcggca cgcttttcgc
      721 cggactgccg cagccgtgcg aggtagggcg ctaccattcg ctcatcgccg cgcccgcgag
      781 cctgcccgcc gttctcgagg tgagtgcgcg cacggacgag ggcgagatca tggcggtgcg
      841 gcaccgcagc ctagtggtcg agggcgtgca gtttcatccc gagagcatcc tcacacccga
      901 gggcccgaag atgctggaat ctttcctgcg ctaccgcgcc ggcgtgcgcg acggagccgc
      961 tcatgtcggc gccgcgtgag ctgctgcagc agctgatcga gcggcgcgat ctcgcgcagg
     1021 cgcaggcgga agcactgctc gcgcatctca ccgatccgca gctgccgccc gccatggccg
     1081 gagcgctgct cgctgcgcta tcggccaagg ggcttgctgc taccgaagtg cgtgggctcg
     1141 cccaggcgat gcgccgcctg gcgcgccgtc cggatctgcc gcccgatctg cgctcgatcg
     1201 atatcgtggg caccggcggc gatgcttccg gcagtttcaa catctccacc ggcacggcgc
     1261 tgctcaccgc cgcgtgcggc gtgccagtgg tgaagcatgg caaccgctcg gtgtcgagcc
     1321 gttgcgggag tgccgatgtg ctcgaggccc tcggactcac agtgccgctc gatgccgcaa
     1381 ccgcgcgtgc ctgcttcgct gcgacgcgct tcacctatct gttcgcgccg tactaccacc
     1441 cggcgatcgg ccgtgtcgcc gccatccgtg ccgccctcgg tgtgcgcacg gtcttcaata
     1501 ttctcggtcc cctggtgaat ccggccgagc cgcctctgca tctggtgggc gccttcagcc
     1561 tggaagtcgc gcagctcatg gccgaggctt tccaggggct tcccatcgag cgcgccttcg
     1621 tggtgcacgg tgcagccgga tgggatgaac caacgcccat gggtcctttc accatcttcg
     1681 atgtgcgccc cggctcggtc gcggtcagcg agcgctccgc cgccgactat gggctgccgc
     1741 cgtgcgccgc ggcggacctc ctcggaggtg atgccgccca caatgcgcaa gcgctcaagg
     1801 cggtgctcta tggcgaggat cgcggcgcgc accgcgattg cctgctgctc ggcacggcgc
     1861 tcgcgctcga ggtcgccggt atcgtgcgcg aggcgcgtga aggcgtggct cgcgccgccg
     1921 cagccatcga tgaaggtgcg gcgcggcggc tggtggaatt actgccgcgg cagagcgcca
     1981 gcgtcggcag cgcgcgcggc gggctcggat ccgagggacc ggctgcgtga gctcgtttct
     2041 cgatgagatg gcggcagcga gcacggcgcg cctgcgggct gcgaggacac atctctccga
     2101 ggcggcactg ctcgagcgcg cctcgcacgc cgctgcggca ccgccgctgc gtctctcagc
     2161 ggagggcttc gatctcatcg cggagctcaa gctgcgctcg cccgccgccg gtgcgctagg
     2221 tgctgcggct ggctcgatcg gagagcgcgc cgtggcttac gcgcaagcgg gcgcggctgc
     2281 agtatcggtg ctcaccgagc cgagccggtt cgacggcgct ctctcgcatc tgagtgaggc
     2341 ggcgcgcgcg ctcgcaccct tcggcgtccc cgcgatgcgc aaggactttc tcatcgatcc
     2401 ctaccaggta ctcgaggcgc gggcggctgg cgcgggcggt gtgctgttga tcctgcgtat
     2461 gctctccggc gagacgctcg ggacgctcat cgagtgcgcg cgagcacagg ggctcttcgt
     2521 gctcctcgaa gccttcgatg agagcgatct cgagcgcatg cggcaggtca tcgccgcgga
     2581 cgccggccgc agcacgctgc ttgccggagt gaattgccgc gacctcggca ctctcgaggt
     2641 cgtacctcaa cgcctgttcg agctcgcccc gctcatgccg ccgcaggtgc cgcacgttgc
     2701 cgagagcggc atcgaggttg cggccgatgc cgcgcggctt gccgcggcgg gatacgatct
     2761 cacactggtc ggcagcgcgc tcatgcgcgg cgcggatccc ggagcgctgg cacgctcgtt
     2821 gctcgcggcc gggcgcagcg cacgcggggg atcgtaaatg tggatcaaga tctgcggcat
     2881 gaccacggcg gccgcggtgg acgcggcgct cgcggcggag gtcgacgcca tcggcttcgt
     2941 gttcgcaccc tcggtgcgcg agctgacccc ggtcgccgcg gccgcgctgg cgcgcccggc
     3001 gcgcgggcgg gtgcggtgtg tagcggtcac gcaccatccg acgcagacgg cggtggatga
     3061 gatcgtgcgc gagtttgtcc cggatctcct gcagagcgat gccgaagact tcgcgcggct
     3121 gcgcctgccc gcagcgctcg agaccctcgc tgtcttccgc gagggcgccg caatatccgt
     3181 gcctcctccg gcgcggattc tctacgaggg gccggcgagc ggccgtggcc agctgatcga
     3241 ttggagcgcc gcgcgcgcac tcgcgcgcct cagcgagctg gtgctggccg gtggtctcaa
     3301 cgaggggaac gtcgccgccg cgatcgcgca ggtggacccc ttcggagtcg atgtatcgag
     3361 tggcgtcgag gagcgcccgg gcgtaaagag ccctgccgcc atcgtggatt tcgtggccgc
     3421 cgttcgcgcc gccggttcca tttagccaga ggagaaccaa gatgtccgca accgttgcac
     3481 tgcccgatgc cgcacagctg ctcgcggcgg cggtggccgg tcagttcccc gacgagcgtg
     3541 gccgtttcgg ccccttcggg ggtcgctatg tgcccgagac actcatcccg gctctcgagc
     3601 ggctcgagcg cggcgtgcgc gcacacctgc actctcagga atttcaggcg gagctcagcg
     3661 cgcagctcac cggttgggtc ggccgcccta cggcgctcac ttgcgcgccg cgcctctcgg
     3721 caagctgggg tgcgcaagtg tggctgaagc gcgaggatct cgcgcacacg ggcgcgcaca
     3781 agatcaacaa tgctatcggc caggcgctgc tcgcccaccg cctgggagca aagcgcatcg
     3841 tcgccgagac aggagcgggg cagcatgggg cggcgagcgc cgcggcgtgc gcacgtctgg
     3901 ggctgccgtg caccgtatac atgggttcca tcgacatgga gcggcaggcg cccaatgtcg
     3961 ggcgcatgcg gctgctcggc gcccgcgtcg tgcccgtcga aagtggtgat cgcacactgc
     4021 gcgcggcgat cgatgaagcg ctacgcgatt gggtgtccga cccactcgat acctattatc
     4081 tgctcggctc ggtcgtgggc ccgcacccgt acccctattt ggtgcgcgag ctccaggcgg
     4141 tgatcgggcg cgaggcgcgc gcgcagatcc tcgccgccac cggccggctg cccgatgcgc
     4201 tcatcgcttg cgtcggcgga ggctcgaact ccatcggtgc ctttcacgcg ttcgttgccg
     4261 acaccgcggt cgagatcatc ggtgtggaag ccggtggtcg cggcgcagcg ctcggtgata
     4321 atgccgctac ggtggcgcac gggcggccgg gagtgctgca cgggagctat tccctgctgc
     4381 tgcagaacgc ggacggtcag atccaggaaa cgcattccgt gtcggcggga ctcgactacc
     4441 ccggtgtcgg tcccgagcac gcactgctcg cggcttcggg acgcgtgcgc tatgagatcg
     4501 cgagcgatgc ggaagcgctc gagggcgtgc ggcagctgtg cgagcgtgaa ggcatcctgc
     4561 ccgcgctcga gagtgcccac gcgctcgcgg gcgctgcgcg ctgggcacgc cggcaccccg
     4621 ggggcacggt gctcgtctgt ctctccgggc ggggcgacaa ggatatgtcg acgctgcagc
     4681 agacgctgct ggcagcggag cgcgcgtgag cccgcgcgag cgtatcagca gcgccatccg
     4741 cgcagccgct gcacgcggcg agccggcact cgtggcttac ctcaccgccg gcttcccgag
     4801 ccgcgcgcgc tttgccgagc acttgcggac actcgccgat gccgccgacg tgatcgaggt
     4861 cggtgtgcct ttcaccgacc cgatggcaga tggagtaacc atccagaggg cgagcctcgc
     4921 ggccctggct cagggcgtga gcctcgcctg gatcctggag gagattgccg cactcggtgg
     4981 actcgcggct ccgctcgtgc tcatgagtta cctgaatccc ctgctcgcct tcggcatcgc
     5041 gcggctcgct gagagcgccg cgcgcgcagg agtgagcggc ttcatcgtgc cggatctgcc
     5101 cctcgatgaa agcagcgcgc tgcgccacgc gctcgatgag cgcgagctcg cactcgtgca
     5161 gatggtgact ccggtcaccg ggccgcagcg gctcgcgcag ctctgtgctg gtagtgaggg
     5221 tttcgtgtat gcggtcacca tgaccggcac caccggccgc aacgtcgccg tgcccgagca
     5281 ggtgctcgag tacctcgatc gcgtgcgtgc tgtagcgcag ctgccggtgt gcgccggatt
     5341 cggtatccgc agccgcgaac aggtcgagcg gctgcgcggt cacgtcgatg gagtggtggt
     5401 cggatcggcg ctggtagaag tgctggagcg tggcgaggat gcgggtgcct tcctgcgagg
     5461 attgcggcct gcctgagctc tttgcaccct cctggcgtat acgcccgacg attcttgaac
     5521 cgttccccga ttcgcggaag ccgctcggtc gagcgcggta ccgcgagctc tgcttcgatt
     5581 gcggtcccgc tgatgttaca taaccgctct gaaattacat aagcgcccga ccgacgtcgc
     5641 aatcttgcga cggtctcgag tactgacgct ccgacccgcg ttcccccctt actgcgcagc
     5701 ttgcaaccac acgtcacgcg agcagaatcc atacgctgcg tgctgtcgaa acatgcaccc
     5761 aagatggcgg caccggacct gaactccaaa caagatttac ggggagattt cgcttatgcg
     5821 tacgcagact tggtcttgcc gcgcttttct cagcgccgga cttctgttgt tgagcagctc
     5881 tctggcctgg ggcgcgcccc ctcacgctga gttcctgcaa tgtaacgagc cgggcggaat
     5941 tctgtgcgcg gagcagcgtg agaatcctgg gggcaaagag tattacgtcg gtcacgacga
     6001 gcctgcagtg ctcttttact cgaacacacc gggcgccggt ttcaacaatg tctaccgcat
     6061 aaggctgccg aaggatccac cggcacttcc caagaagaat ggcagcggag gaacctggaa
     6121 ttttcagttg cacccggctt tctggttcgg catggccatg tgtgacgatc agggtaatcc
     6181 caacccagga gggtcgccca gttttgccaa catcccgtgc actcctgaca gtgaccgcaa
     6241 catcttcgac agcccggacc cgaccgcgcc cgactacatc gggcggcacc cgggcacggc
     6301 attcatggag atgcagttct acccgccggg ttggattacc agcaacagcg gtgcggtcga
     6361 tgccgagagc gcgacgaaat ggaccgcagc gctcaacatc gacagctttc agtcaaatga
     6421 caacgtggtc tgcggcacgg aaggcccaaa ctgcttaggc ggagtcccac ctggcccgag
     6481 cgcgattcag gccaataacg ccgcttgttc agcgatcact tttgacgaat acgtcaactt
     6541 ttcctttatc cagactgatg gcgttccaag cgcctcaccg agcccatacg acctggccct
     6601 gaacacgacc aacgccaaaa cgctccgcat ggatggaggc gatgagctca ttgtgacaat
     6661 caaggacacc cacagcgggc tcatggtcac gatccaggat ctcaccagcg gtaatagcgg
     6721 tttcatggtg gcgagcggtg cgaatggatt tgcctcggtg aagtgggacc ccagcggaga
     6781 caattgtctc cgagctactc acgagctcag gcacaacttt catccggcct acgccacctc
     6841 aagcgagcac acacgcgtgc cgggtgcggc gcattcgtac aacatcgctt tctcggatga
     6901 gatcgggcac ttcgagtatt gcaacggggt tgctaaggag ggcggtgcct gcacgtctgc
     6961 ttccgggagc gacaagaagg ccgacgtcga tgacggagcg ttcggcgggt gcttcgacgc
     7021 cgggtttgcc gcgtcgctcg gtctgattca aatcggcggc tgcctcgata gcgatacgga
     7081 cttcgacggt gttccctacc ggaataacac ctggccgggc tcatacagcg atcccgtcca
     7141 ggatgcgctc ttccatgcgc agccggtgat cttctccagc ccgttgctca ctgacagcgg
     7201 cggtcacctg aggaatttca gtcgagtcgc cttcgagacc gacatgccgc gcatcgagtt
     7261 cgctaccaac ccgccatgtc agcggcacct gtcgaacccc gcggatccga acccgggact
     7321 gggctgcgtc aatcctcccg tgggcgcggc tttctatccg ttttacagca cgcgctcgga
     7381 tgacggagtt tgcctatggc aggagggtgg ccccttcatt ccaggaacgg tgaataattt
     7441 tggcgcaagc tcacaggcgc agttcgggtc cattcctctg gcgctcttct accccgcggt
     7501 gaatatcatg ccccaataca tctacgagga ctttcgcaac attctgacga cgaatccttg
     7561 tccggcccct acgggcgact aggcaatccc agcctggact gtcggaagcg ccagaaaggg
     7621 cgggtaacac cgccctttct gtttatgcgg caagagttgt ccgttggggc gggtcgcaag
     7681 cccgcatttg cctccggcct ccagaacgcc cattctcacg cggctcatgc aatccctgac
     7741 tatcgccacg cgcttccgcg gcccccccga gtctgccaac gggggctatt tcgccggact
     7801 cgtggcgacg ctcgccccac gcaccgtgag tgtgcggctg ctgaagccgc cgccgctgaa
     7861 tatcgggctg gcggtgagcg cgctcgagga tgggggtctg cgagtgctgc gcggtgagga
     7921 gcccatcggc gaggcgcatc cggcggtcct cacactcacc gtgccaccgc cacccgatta
     7981 cctcgaggcc gtggaagcct cgcgccacta cgcgggcttc cgctaccatc gcttcccgag
     8041 ctgcttcgtg tgtggcacac agcgggcgcg cggcgatggc ctgcggatct tcgccgggcc
     8101 gatcgcggag cgcgacgtgg tggccgcgcc ctgggtaccc gacgcctcgc tcgatgccgg
     8161 cgatggcaag gtgcgggccg aattcatgtc ggccgctctc gattgcccgg ggtactacgc
     8221 ggtcgccccc gacgatcgca tgatgctgct cgcggaattc accgcccacg tcgatcggcg
     8281 ggtgcacatc ggcgaatcct gcacgctcat cggctggtgc atcggcagcg acggtcgcaa
     8341 gcacgaggcc ggcacggcgc tgttcgatgg caagggtgag ctgtgcgggc gtgcgcgggc
     8401 actgtggatc gagccgcgcg cgacggtgag cgagctgtcg gtgaagtgag gcgctggaca
     8461 cgcgctcatg catgtctcgc gcgcaatagt tcaccatttg gttaactatt ggcacggaca
     8521 cttcatgcag actgcctctg tgagcccggc tgcggcggtg atgccttacg aggagcgcgc
     8581 cggcagcgcc atatgtgcgt gccgccagtc cccatagcgg tagtagagca ccgccagcag
     8641 cgccgccagg agcgaggaca cggggaagct ccaccacacc gcatcgacct ggtagcgccc
     8701 gaggaacgcc tcggcgaacg ggaaacgcac cagcagcagt gcgatggtca ggatgatcag
     8761 cggggcgatg acggcgccgg tcgcacgcac gacgccgaag agcgtgagcg agatgccgaa
     8821 gaagatgaag gagggggtca cgatacggtt catgtgactt gcgatcgcga gtgcatctga
     8881 agccgccggg aggaacaggc cgaacaccca gctgttgagt agctcgatcg cgagcacgac
     8941 tgagccggtg agcagcacgc tgtacagcgt gccgacgcgc gcgatggtag tgacgcgatg
     9001 ccacttgtgc gcgccgacgt tctgagcggt catggccgac actgccatgc cgatcgcgaa
     9061 ggcgggcatc tggatgtaat tccagatctg cagcgctgcg ccgaatgcgg ccgtggtatc
     9121 gacgccgaag cggttgacga gggtgatcat gagtacgccg ctcagcgaga gcaccagcat
     9181 ctgcgcgctc atgggaattc ccttgcgcac cagcgtgccg atgagactcc cattgggctt
     9241 gagcaggtgc agctcctggc ggtgcaggca gagaatgtgg tggcggcggt atagatggcg
     9301 gatcaatgcg atgaggctca ccacctgcgc cacgaaagtg gcgagcgcgg agcccgctat
     9361 gccgaggcgc ggaattggcc ccagcccgaa gatgaacacc gggttgagca cgatgtcgat
     9421 gcccaccgac agcaacatga agtagaaggg agtctttgag tcgccggcgc cgcgcagcac
     9481 ggccatcacg aaggcataca tgtacagcca cggcagtgcg aggaagatga cgcgcatgta
     9541 ggcaaccgcg agcggcagcg agtccgcggg cgtgctcatg gcgatgagca gcgggcggca
     9601 caacagcagg ccggcgatgg ccatagttac cgagatgccg gcgaagaaag tggcgctggt
     9661 gccgacgacg cgcttggtct cgtgcaggtt gttggcgccg atgcattgtc cgatgaggat
     9721 agtggcggcg agcgccatgc cgaatgcggc gccgatcagc aggaacatca cggtgttggc
     9781 gttggaggtc gcggtcagcg ccgcctcgcc gaggaagcgc cccacccaga tcgagttcac
     9841 cgagccgttc agcgactgca ggatgttggc aaacaggatg gggagcgcga agcggaacag
     9901 gccgaccgga atcgagcctt cggtgagtga gcgttgcact taattagtgc gccacagccc
     9961 gtaaacgact ccggcagcgc cggccacgac caggcaccac ccgacccagg ccggctcgcg
    10021 cgccacggcg agccacacca gcccgccgag cagcagcgcc gcaccgagtg tcaccgcatc
    10081 gcgccgacgt gcggcgcgcg cgagcgcgct gcgcagctcg aggctgtcca cggcaggggc
    10141 tggcgcgcgt accccggcga gctcgcgctc caacagcctg ccgagcagca acggcaggcc
    10201 gcgcgcggcc tccatgagtc cgggtgtgcg ggcgcgcagc tcgcgcgcga gcgcacgcac
    10261 gctggtgcgc tcgcgcatcc attcgcgcag gatcggagtg gcggtggccc acacgtccag
    10321 atccgggtac aggtcgcgcc cgagtccctc cacgttgagc agggtcttct gcagcagcat
    10381 gagctgcggc tggatctgca tgttgaagcg ccgtgagatc tcgaacaacc gcagcagtat
    10441 gcgtccgaag gagatgtcct tcagcggccg gtcgaagatc ggctcgcaca c
//
LOCUS       DATDMP010000318         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_938747, whole genome shotgun sequence.
ACCESSION   DATDMP010000318 DATDMP010000000
VERSION     DATDMP010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938747"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VI195_05505"
     CDS             <1..485
                     /locus_tag="VI195_05505"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823875.1"
                     /translation="AEDSVYMGQTPPYRTANRYITSASELLALPGFGRDRYLALAPYV
                     TALPYKTQLNVCTAKDKVLDAFLGAGQTDFSSTAGQLDKNRAQGSTGCFPTIANFQAA
                     FNDPKQWAAVSGKFSQTSTYFRLTSFITVGSAEFNLYSLLYRDATGAVRPIQRSFTPD
                     "
     gene            557..1792
                     /gene="gspL"
                     /locus_tag="VI195_05510"
     CDS             557..1792
                     /gene="gspL"
                     /locus_tag="VI195_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEY6823876.1"
                     /translation="MADWLLLRLPRADTALATWLVVDSRGAPSSPPQSGPLTLAAARA
                     PGRRVCVLVSGAEVLLAEPEVPVKAGAKLQQLVPYALEEQLAEDIEDLHFALGKRASE
                     TARVPVAVVARALLDQWLTELRGAGIEPDAVYAESELLPQNPGQAVALLEADTVSVRP
                     PGGTPVTLPGDALGEALEIARTGADASATGGRGLILYTGAPEWHQHSAQVEAARPHFD
                     GIKVQLLAGGPLALYAQQLPSTAAINLLQGPYAPVSSRGVGLKAWRVAAILLAALIGL
                     HIIGKTAELQVLKRHERQLDASIRDTFRSSMHSEASATEARRLMEKRLAGARGAGEGL
                     LPALQALAQARGNAPGTSVQSLNFHTGTLEMKLSAPDATSLDRLSQSLRSNGWHADLT
                     GGSNSGSAYEGRIQMHSGA"
     gene            1802..>2210
                     /gene="gspM"
                     /locus_tag="VI195_05515"
     CDS             1802..>2210
                     /gene="gspM"
                     /locus_tag="VI195_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY6823877.1"
                     /translation="MKLSLHSFSLGSLSLRSLSPEALAALSPRERRLLLVGAIAAVAI
                     LIFGILIPLDRGVAHAQERLAKKRADLSWMQSVAPQIALLPPPSSNGESLLVVIDRSA
                     RESGLASALSGSEPGSSGNLSVRLEKAPFDVLVG"
BASE COUNT          345 a          775 c          727 g          363 t
ORIGIN      
        1 gcgcggagga cagcgtttac atgggccaga cgccgcccta ccgcacggcg aatcgctaca
       61 tcacgagcgc gagcgagttg ctggcgctac cgggcttcgg ccgcgaccgc tacctcgcgc
      121 tcgctccgta cgtgaccgca ctcccctaca aaacgcagct caacgtgtgc acggcaaagg
      181 acaaggtgct cgatgcgttc ctcggcgcgg gtcagaccga tttcagctcg acggccgggc
      241 agctcgacaa gaaccgtgcg cagggctcga cgggttgctt cccgaccatc gccaatttcc
      301 aggcggcttt caacgacccc aagcagtggg ccgccgtgtc gggcaagttc agccagacct
      361 cgacctactt ccgcctgacg agcttcatca ccgttggcag tgcggagttc aatttgtaca
      421 gtctgctgta ccgggatgcg actggcgccg tgcgtccgat ccagcgcagc ttcacccccg
      481 actgaggccg cgcgagctct ccccatagtg acgcaatcga caagcgtgaa tgcccgaatg
      541 atgacactat ccgctcatgg ctgactggct gctgcttcgg ctgccgcgtg cggacaccgc
      601 acttgcgacc tggctcgtgg tcgattcccg cggggcaccc tcgagcccac cccagagcgg
      661 gccgctaacg ctcgcggcag cccgcgcacc cggccgccgc gtctgcgtgc tggtctcggg
      721 cgcggaggtg ctgctggcgg agcccgaagt gccggtaaag gccggagcca aactgcagca
      781 gcttgtcccc tacgccctcg aggagcagct cgccgaggac atcgaggatc tgcactttgc
      841 cctcggcaag cgcgcgagtg agacggcgcg tgtacccgtg gcggtggtcg cgagggcgct
      901 gctcgatcaa tggctcaccg agctgcgcgg cgcgggcatc gagcccgatg cggtgtatgc
      961 cgagagcgag ctgctgccgc agaatcccgg acaggcggtc gcgctgctcg aggccgacac
     1021 ggtgtcggta cgcccgcccg ggggaactcc ggtcaccttg cccggcgatg cactcggtga
     1081 ggcgctcgag attgcgcgca ccggcgcgga tgccagcgca accggcggac gcggcctgat
     1141 cctttacacc ggagcgcccg agtggcatca gcactccgct caggtcgaag cggcgcgacc
     1201 ccatttcgat ggcatcaagg tgcagctgct cgccggcggt ccgctcgcgc tctacgccca
     1261 gcagctgcct tcgacggcgg ccatcaatct gttacagggc ccgtacgcgc cggtctcttc
     1321 gcgcggggtc gggctcaagg cgtggcgcgt ggcggccatt ctcctcgcgg cgctcatcgg
     1381 actgcacatc atcggcaaga ccgcggagct gcaggtgttg aagcgccacg agcggcaact
     1441 cgatgcctcc atccgcgaca ccttccgcag ctccatgcac agcgaggcga gtgcgaccga
     1501 ggcgcggcgc ctgatggaga agcgcctcgc cggggcgcgc ggtgccggcg agggcctgct
     1561 gccggcgctg caggcgctcg cgcaggcgcg cggcaatgcg ccgggcacga gcgtgcagtc
     1621 actgaatttc cacaccggaa cgctggagat gaagctttcc gctcccgacg ccacgagtct
     1681 cgatcgtctc agccaatccc tgcgcagcaa cggctggcac gccgatctca ccggcggatc
     1741 caactccggc tccgcctacg aagggcgcat ccagatgcac tcgggggcct gaggcagcgc
     1801 catgaagttg tcgctccact cattctcgct cggatcgctc tcgctgcgat cgctctcgcc
     1861 ggaagcgctc gcggcgctct caccgcgcga gcggcggctg ctgctcgtcg gggcgattgc
     1921 agcggtcgcg atcctgattt tcggcatact gatcccgctc gatcgcggcg tcgcgcatgc
     1981 gcaggagcgg cttgccaaaa agcgtgccga cctcagctgg atgcagtcgg tcgcgccgca
     2041 gatcgctctg ctgccaccgc cctcctccaa cggcgagtcg ctgctggtgg tcatcgatcg
     2101 ctcggcgcgc gagtcgggtc ttgcgagcgc cctctccggc agcgagcccg gcagcagcgg
     2161 taatctctcg gtgcgtctcg agaaggctcc cttcgatgtg ctcgttggtt
//
LOCUS       DATDMP010000319         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_272401, whole genome shotgun sequence.
ACCESSION   DATDMP010000319 DATDMP010000000
VERSION     DATDMP010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272401"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VI195_05520"
     CDS             complement(<1..805)
                     /locus_tag="VI195_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY6823878.1"
                     /translation="MNRQPTAPPAAPVRALLRHFQAQRPLRGGSLLITIFGDAIAPRG
                     GAVTLGSLIRLAAPFGLAERAVRTAVARLARDGWLSSRRAGRRSEYRLTAGGAARFAE
                     ATRRIYGENPRSWSGRWTLVLLPAGAGPRREVHAELRWLGFGQLNAALFAHPSCTVAE
                     ARAWLRRIDGVRGALLLSSSGSGLAADREIVARGWDLAELARRYRRFVKRFCALEPVF
                     AADGVAPETAFLVRTLLIHEYRKIHLQDPMLPPALLPRDWVGGAAYEVCR"
     gene            893..1900
                     /gene="paaA"
                     /locus_tag="VI195_05525"
     CDS             893..1900
                     /gene="paaA"
                     /locus_tag="VI195_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY6823879.1"
                     /translation="MYTQALDTHGKEPAGSTEAEPQAARFQGRVDADEKIEPKDWMPE
                     AYRQTLIRQISQHAHSEIVGMLPEGNWISRAPTLKRKAVLIAKVQDEGGHGMYLYSAA
                     ETLGISRAQMLEQLLTGAAKYSSIFNYPTLTWADIGAIGWLVDGAAIMNQIPICRCSY
                     GPYARAMVRICKEESFHQRQGFEIMLTLTQGSQAQRAMAQDALNRWWWPSLMMFGPSD
                     ADSKHTAQSMRWKIKRFSNDELRQKFIDITVPQAHFLGLTIPDDELHWDEATQHYHVG
                     PIDWSEFYAVLKGNGPCNRERLESRQRAHEAGRWGREAAAAHAERERSRSAAQAAGEA
                     A"
     gene            1897..>2099
                     /gene="paaB"
                     /locus_tag="VI195_05530"
     CDS             1897..>2099
                     /gene="paaB"
                     /locus_tag="VI195_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEY6823880.1"
                     /translation="MSELDWPLFEVFIRSRSGLEHKHVGSLHAADARMAIEHARDVYT
                     RRQEGVSLWVVRSADIVASDPGEA"
BASE COUNT          381 a          705 c          730 g          283 t
ORIGIN      
        1 gccggcaaac ctcgtaggct gcgccaccga cccagtcccg cggcagcaat gccggcggaa
       61 gcatcgggtc ctgcagatgg atcttgcggt actcgtggat cagcagcgtg cgcacgagaa
      121 atgcggtctc gggggcaacg ccatcggctg cgaagacggg ctcgagcgcg cagaagcgct
      181 tgacgaagcg ccggtagcgg cgggcgagct cggcgaggtc ccagccacgt gcgacgattt
      241 cacgatcggc agcgaggcct gagcccgagc tgctcagcag cagcgcaccg cgcacaccat
      301 cgatgcggcg cagccatgcg cgtgcctcgg ccacggtgca ggacggatgc gcgaaaagtg
      361 cagcgttcaa ctgaccgaag ccgagccagc gcagctccgc gtgcacttcc cgccgcggtc
      421 cagcgccggc aggcagcagc acgagcgtcc agcgaccgct ccaggagcgg gggttctccc
      481 cgtatatgcg ccgcgtcgcc tcggcaaagc gcgccgcgcc accagccgtg aggcggtact
      541 cgctgcgccg tccggcgcgc ctcgaggaga gccagccatc gcgcgccagg cgcgccaccg
      601 cggtgcgcac cgcgcgctcg gcgaggccaa agggggcggc gaggcgaatg aggctgccga
      661 gtgtgaccgc gccgccccgc ggagcgatgg catcgccgaa aatcgtgatg aggagcgagc
      721 cgccgcgcag cggccgctgt gcctggaagt gccgcagcag cgcccgcacg ggagccgcgg
      781 gcggggcggt cggctggcgg ttcatgcgcc aaaattgtga cacgaaaatc gatcgctctg
      841 tgctaatctt gtgtcatatc tgcggcccag gtccgcagcg ggcggggcac gcatgtacac
      901 ccaggcactc gatacccatg gcaaggaacc cgccggcagc accgaggcgg agccgcaggc
      961 cgcgcgcttt caggggcgcg tcgatgcgga cgagaagatc gagcccaagg actggatgcc
     1021 ggaggcctat cgccagaccc tgatccgcca gatctcgcag cacgcgcact cggagatcgt
     1081 cggcatgctt cccgagggca actggatcag ccgcgcaccg acgctcaagc gcaaggccgt
     1141 gctgatcgcc aaggtgcagg acgagggcgg ccacggcatg tatctctaca gcgccgccga
     1201 gacgctcggt atctcgcgcg cccagatgct cgagcagctg ctgaccggcg ccgccaagta
     1261 ctcgagcatc ttcaactatc cgaccctcac ctgggcggac atcggcgcca tcggctggct
     1321 ggtggacggt gcggcgatca tgaatcagat cccgatctgc cgctgctcct acggtcctta
     1381 cgcacgtgcc atggtgcgca tctgcaagga ggagagcttc caccagcgcc aggggttcga
     1441 gatcatgctg accctcacgc agggcagcca ggcgcagcgc gccatggcgc aggatgcact
     1501 caatcgctgg tggtggccct cactcatgat gttcggtccg agcgacgccg actcgaagca
     1561 caccgctcag tccatgcgct ggaagatcaa gcgcttcagc aacgatgagc tgcggcagaa
     1621 gttcatcgac atcaccgtac cgcaagcgca tttcctcggc ctgaccattc ccgacgacga
     1681 gctgcactgg gatgaggcca cgcagcacta ccacgtgggc ccgatcgact ggagtgagtt
     1741 ctacgcggtg ctgaagggta acggaccgtg caatcgggag cggctcgagt cccgtcagcg
     1801 cgcccatgag gccgggcgtt gggggcgcga ggcggccgcg gcgcacgccg agcgcgagcg
     1861 cagccgcagc gcggcgcagg ccgcaggaga ggccgcatga gcgagctcga ctggccactg
     1921 ttcgaggtat tcatccgctc acgctcggga ctcgagcaca agcacgtcgg cagcctgcat
     1981 gccgcggatg cgcgcatggc aatcgagcac gcgcgcgatg tctacacgcg ccgccaggaa
     2041 ggtgtgagcc tgtgggtggt ccgctcggcg gacatcgtgg cgtcggatcc cggggaggc
//
LOCUS       DATDMP010000320         4915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1273375, whole genome shotgun sequence.
ACCESSION   DATDMP010000320 DATDMP010000000
VERSION     DATDMP010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273375"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /gene="rpsA"
                     /locus_tag="VI195_05535"
     CDS             complement(<1..1322)
                     /gene="rpsA"
                     /locus_tag="VI195_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY6823881.1"
                     /translation="MTESFAQLFEQSLANQRIRPGMILTGLVVEVTSDVVIVNVGLKS
                     EAVIPLEQFKNERGEVEVKAGEEVEVALDSVEDGTGETRLSREKAKRARTWTRLEEAF
                     NKSEIVTGVITGRVKGGFTVEIENVRAFLPGSLVDVRPVRDTSYLEGKPLEFKVIKLD
                     QKRNNVVVSRRAVVEQEFSAERSALMDNLQEGAVVRGTVKNLTDYGAFVDLGGIDGLL
                     HITDMAWKRVKHPSEVVKVGDEIEVRILKFDRERSRVSLGLKQLGADPWQNIARRYPP
                     GTRVFGKVTNIADYGAFVEIEDGVEGLVHVSEMDWTNKNVNPAKVVHTGQEVEVMVLD
                     VDEERRRISLGLKQCQANPWKEFAENYNRGDKVAGQVKSITDFGIFIGLPGNIDGLVH
                     LSDISWDQPGEEAVRNYQKGQQLEAMVLSIDPERERISLGIKQLAKDP"
     gene            complement(1467..2159)
                     /gene="cmk"
                     /locus_tag="VI195_05540"
     CDS             complement(1467..2159)
                     /gene="cmk"
                     /locus_tag="VI195_05540"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY6823882.1"
                     /translation="MSKSPACPIVTIDGPSGSGKGTISRAVARTLGWHLLDSGALYRL
                     VALAGTAAGLAPEDARAHAALARRMDVAFTVASDGSERISLDGRDVTQSIRSEEAGQG
                     ASRVAAWPGVRQGLLARQRAFAVPPGLVADGRDMGTVVFPRAELKIFLTATPEERALR
                     RHKQLKDKGSDVSLPALSREIAERDLRDQTREVAPLKAAPDACVIDSTGLSVEAVVQR
                     VLELGAARRLWS"
     gene            complement(2174..3499)
                     /gene="aroA"
                     /locus_tag="VI195_05545"
     CDS             complement(2174..3499)
                     /gene="aroA"
                     /locus_tag="VI195_05545"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY6823883.1"
                     /translation="MSAAPLRGWRVAPGGAVTGVFSIPGDKSISHRALMLGALAEGQT
                     RITGFLAGEDCLATARALAALGVRIERPSETEVRVHGRGRGGLAPSSSPLDLGNAGTA
                     MRLMMGVLAPQSFDSTLIGDASLMRRPMERVAEPLRRMGARIDTQQGRPPVRIHGTPQ
                     LKGISYRLPVASAQVKSAVLLAGLSASGHIELTEPAPSRDHTERMLRAFGVRVAQERG
                     VISLEGGQSLRGTQLAVPADFSSAAFFLVAGVLAAPGGLRLTNVGVNPTRTGLLELLR
                     LMGADIRVVAHSGTTAHSVEPVADIEVRAGALRGITVPDALVPLAIDELPVFFIAAAA
                     ASGETLVRGAGELRVKESDRLAAMAAGLGVLGVEHELLPDGLRIRGRAEFSGGAIDSL
                     GDHRIAMAFAIAALRARAPIEIADVANVATSFPGFEACARRAGLGITTL"
     gene            complement(3496..4629)
                     /gene="pheA"
                     /locus_tag="VI195_05550"
     CDS             complement(3496..4629)
                     /gene="pheA"
                     /locus_tag="VI195_05550"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY6823884.1"
                     /translation="MARHPPAHRARRPAPSRPRGLEDVRGRIDAVDEQLHRLLNERAR
                     LAQQVGISKAHDGHTVDFYRPEREAQVLRQARARNAGPLRDEEVLRLFREIMSACLAQ
                     QEPLKVAFLGPEGTFTQTAVLNHFGHSVRALPLASIDEVFHEVEAANADFGVVPIENS
                     TEGTVNHTLDRFLSSPLKICGEVELRVRHHLMGAMSALSRIVRVCSHPQSLAQCRQWL
                     EEHLPGVEQVPVSSNAEGARRARDEKGSAAIAGETAAEVYGLKVLAAEIEDRADNTTR
                     FLILGRKLFAPSGEDRTTLLVSVGHTDAPGALYRLLEPLARHRVSLTRIESRPSRRRK
                     WDYVFFMDLEGHADEPHVARALTALKKRASLFRVLGSYPRAVQ"
     gene            complement(4731..>4915)
                     /locus_tag="VI195_05555"
     CDS             complement(4731..>4915)
                     /locus_tag="VI195_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HEY6823885.1"
                     /translation="ENGNIKNSVNYPDTILPRVPNTTRLGVANRNVPNMVGQISTCLA
                     TQGINIADLLNKSRGE"
BASE COUNT          821 a         1680 c         1603 g          811 t
ORIGIN      
        1 aacgggtcct tggcgagctg tttgatcccg agggagatgc gctcccgctc cggatcgatg
       61 gacagcacca tggcttccag ctgctggccc ttctggtagt tgcgcacggc ctcctcaccc
      121 ggctgatccc aggagatgtc cgagaggtgc accaggccgt cgatgttgcc cggcagcccg
      181 atgaagatgc cgaaatcggt aatggacttg acctgcccgg cgaccttgtc gccgcggttg
      241 tagttctcgg cgaactcctt ccacggattc gcctggcact gcttgagacc gagcgagatg
      301 cgccggcgct cctcgtcgac atcgagcacc atgacctcga cttcctggcc ggtgtgcacc
      361 accttcgccg ggttgacgtt cttgttggtc cagtccatct cggagacgtg caccaggccc
      421 tcgacaccgt cctcgatctc gacgaaggcg ccgtagtcgg cgatgttggt gaccttgccg
      481 aacacgcgtg tgcccggtgg gtagcgccgg gcgatgttct gccacgggtc ggcgccgagc
      541 tgcttgagac ccaggctcac ccgcgagcgc tcgcggtcga acttgagaat gcgcacctcg
      601 atctcatcgc ccaccttgac gacttcggag ggatgcttga cgcgcttcca tgccatatcc
      661 gtgatatgca gcaggccgtc gatgccgcca agatcgacga acgcgccgta gtcggtgaga
      721 ttcttgaccg tgccgcgcac cacggcgcct tcctgcaggt tgtccatgag cgccgagcgc
      781 tccgcggaga actcctgctc caccaccgcg cggcgcgaga ccacgacgtt attgcgcttc
      841 tgatcgagct tgatgacctt gaactcgagc ggcttgccct cgaggtagga ggtgtcgcgc
      901 accgggcgca catcgacgag cgagcccggc aggaatgcac gtacgttctc gatctcgacc
      961 gtgaagccgc ccttgacgcg tccggtaatg actccggtca cgatctcgga cttgttgaat
     1021 gcctcctcga ggcgcgtcca ggtgcgtgcg cgcttggcct tctcgcgcga gaggcgcgtc
     1081 tcaccggtgc cgtcttcgac cgagtcgagc gcgacctcga cctcctcgcc cgccttgacc
     1141 tcgacttccc cgcgctcgtt cttgaactgc tcgaggggaa tgacggcttc ggacttgaga
     1201 cctacgttga cgatgacgac gtcgctggtg acctcgacga cgagcccggt gaggatcatg
     1261 cccgggcgga tgcgctggtt ggcgagactc tgctcgaata gctgggcgaa actttctgtc
     1321 ataccttcac tgatgccatg cacaaaggcg tgtgtgtact ggaaccgcct tccgctgcgg
     1381 tccaggaaaa cataacgggc acccgcttcc tgcgcgtgcc ttccttcgga cttcctgctc
     1441 ccgtgcgtat cgcgacgagc ggccccctaa gaccacaagc gacgagcggc tcccagttcc
     1501 agcacccgct gtaccaccgc ttcgaccgaa agaccggtcg aatcgatgac acaggcatcg
     1561 ggcgccgcct tcaggggggc gacctcacgg gtctgatccc ttaaatcccg ctcggctatc
     1621 tcgcgcgaaa gggcgggaag gctaacatcg gaacccttgt ctttcaactg cttatgccgc
     1681 cgaagggccc gttcctcggg cgtggcggtc aggaagattt taagctccgc ccgcggaaag
     1741 acgacggtac ccatgtcgcg accgtccgcc accagacccg ggggtactgc aaaggcgcgc
     1801 tgccgggcca gcaacccctg acggaccccc ggccaggcgg ctacccggga ggcaccctgc
     1861 ccggcctcct cggagcggat cgactgggtg acatcgcgcc catcgagcga gatgcgctcg
     1921 ctgccatcgg aggcgaccgt gaaggccacg tccatgcgcc gcgcgagggc cgcatgagcc
     1981 cgcgcatcct ccggggcgag cccggcagcg gtgccggcga gggcgaccag ccgatagagc
     2041 gcgccgctat cgagcagatg ccagcccagt gtcctggcga cggcccgact gatggtgccc
     2101 ttgcccgagc cgcttggacc gtcgatggtg acgatgggac aggcgggtga cttcgacatc
     2161 gggacagtgt ccctcagagt gtcgtaatgc ccaagccagc ccggcgagca catgcctcga
     2221 aaccaggaaa actggtggcg acgttcgcca catcggcgat ctcgatcggt gcgcgtgccc
     2281 gcaacgccgc gatggcgaat gccatggcga tgcggtgatc tcccaggctg tcgatggccc
     2341 ccccggagaa ctccgctcta ccgcggatcc gtaagccatc aggcaggagc tcatgctcga
     2401 caccgagtac tccaaggccc gcggccatgg cggcgaggcg atcgctctcc ttcacccgca
     2461 gctccccggc accgcgcacc agtgtctccc cgctggcagc ggcagcggca atgaagaaca
     2521 ccggcagctc atcgatcgcc agcggcacga gcgcgtccgg caccgtaatg ccccgcagcg
     2581 cgccggcacg cacctcgata tccgcgaccg gctcgacgct atgtgcggtc gtgccggaat
     2641 gcgcaacgac gcggatgtcg gcccccatga gccgcagcag ctcgagaagc ccggtgcgtg
     2701 tcggattgac gccgacgttg gtaagccgca gccccccggg agcggccagc actccggcga
     2761 cgaggaagaa ggcggccgag gagaaatccg ccggaaccgc aagttgggtg ccccgcagcg
     2821 actgtccgcc ctcgaggctg atcaccccgc gctcctgggc cacgcgcacg ccgaaagcac
     2881 gcaacatccg ttcggtgtga tccctcgagg gcgcgggctc ggtgagctcg atgtgcccgc
     2941 tggcggagag cccggcgaga agcaccgcgg acttcacctg cgcactcgcc acgggcagcc
     3001 tgtaggagat gcctttgagc tgcggggtac cgtggatgcg gaccggcggc cgtccctgct
     3061 gggtgtcgat gcgtgcgccc atgcggcgca gcggctcggc cacgcgctcc atcggccgcc
     3121 gcatgagcga ggcatcgccg atgagcgtcg aatcgaagct ctggggtgcg aggacgccca
     3181 tcatgaggcg catggccgtt ccggcattgc ccagatcgag cggtgaggag gatggcgcaa
     3241 ggccacctct gccgcggccg tgcacgcgca cctccgtctc ggaggggcgc tcgatgcgta
     3301 cgccgagcgc cgcgagcgca cgggccgtcg cgagacaatc ctcgccggcg aggaaacccg
     3361 tgatgcgggt ctgtccctcg gcgagagcgc ccagcatgag cgcgcggtgc gagatcgact
     3421 tgtcacccgg aatgctgaag acaccggtga ccgcgccgcc gggtgcgacg cgccagccgc
     3481 gcagcggcgc cgcgctcact gcacggcccg tggatacgag ccgagcacgc gaaacagtga
     3541 cgcccgtttc ttcagcgcgg tgagtgcgcg tgccacatgc ggctcatccg cgtgcccctc
     3601 gaggtccatg aagaaaacat agtcccattt acggcggcgc gagggccgcg attcgatgcg
     3661 cgtcaagctc acccgatggc gcgctagcgg ctcgagcaac cgatagagcg ccccgggtgc
     3721 atcggtgtgc ccgaccgaga ccagcagcgt cgtgcgatcc tcaccgctcg gggcgaacag
     3781 cttgcggccg agtatgagga agcgcgtggt gttatccgcc cggtcctcga tctccgccgc
     3841 cagcaccttg agcccataga cctctgcggc cgtctcaccg gcgatcgccg cgctgccttt
     3901 ctcatcgcgc gcgcggcgcg ccccctcggc gttactggag accggcacct gctcgactcc
     3961 cggcagatgc tcctcgagcc actgccggca ctgtgccagc gactgcgggt gcgagcagac
     4021 gcgcacgatg cgcgacagcg cgctcatcgc gcccatcaga tgatggcgta cgcgcagctc
     4081 cacctcgccg cagatcttca gcggagagct gaggaagcgg tcgagggtgt gattgaccgt
     4141 tccctcggtg gagttctcga tcggcaccac accgaaatcg gcattggcgg cctcgacctc
     4201 gtggaacacc tcatcgatcg aggcgagcgg cagagcgcgc accgagtggc cgaagtgatt
     4261 gagcactgcc gtctgcgtaa aggtgccttc cggtccgaga aatgccacct tgagcggctc
     4321 ctgctgcgcc aggcatgccg acatgatctc gcgaaacagc cgcagcacct cctcatcgcg
     4381 cagcgggccg gcgttgcgcg cgcgcgcctg acgcagcacc tgcgcctcgc gctccggccg
     4441 atagaagtcc acggtgtgtc catcgtgcgc cttggagatg cccacctgct gcgcgaggcg
     4501 cgcgcgctcg ttcagcagcc ggtgaagctg ctcatcgacc gcgtcgatgc ggccgcgcac
     4561 atcctcgagc ccgcgtggcc gcgacggcgc tgggcggcgg gctcggtgag cgggtggatg
     4621 ccgggccatg cgcctcatag aatgcgcgcc gagagcacgc catcgatcgc gcgaatgtgc
     4681 gcgagcaact cctcaccgaa cacgccgtcg gcatcgatga gcgtgtacgc ttactcgccg
     4741 cgggacttgt tgagcaggtc ggcgatgttg atgccctgcg tggcgagaca agtggagatc
     4801 tgtcccacca tgttgggaac gttgcgattg gccacgccga gacgggtggt gttcggcacc
     4861 cgcggcagta tcgtgtccgg atagttcacg ctgttcttga tgttgccgtt ctcga
//
LOCUS       DATDMP010000321         7384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_828515, whole genome shotgun sequence.
ACCESSION   DATDMP010000321 DATDMP010000000
VERSION     DATDMP010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828515"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VI195_05560"
     CDS             <1..1073
                     /locus_tag="VI195_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6823886.1"
                     /translation="NALQLLRMQPSAGQSQWAQEVIQRQLAYLTRLVDDLLDVSRITR
                     GKIVLTREPLDVATLITRALETIDPLLRERGHELTLQLPPEPLRVDGDPTRLTQALGN
                     VLGNAAKYTERGGHIHLICTRQGTDVEIRVRDNGIGIPAELLPRIFDLFTQLDRRSDQ
                     SASGLGIGLALVRRLVEMHGGSISAVSAGAGAGSEFVISLPLLSDELAREHDAVREQA
                     REVPALPRRILLADDNPDALESLATVLRLRGHEVYSARNGAAALETAARHVPEVALLD
                     IGMPLVDGYEVARRIRAQEWGKGITLVALTGWGQESDRRRSQEAGFDAHLVKPLDLDK
                     LTAVLAQLPEHVPHVGDLLPAV"
     gene            complement(1350..2447)
                     /locus_tag="VI195_05565"
     CDS             complement(1350..2447)
                     /locus_tag="VI195_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY6823887.1"
                     /translation="MGTNTPRQAPAPRREMPDPALGEREFKRRFLAQFNDPAFDRLAS
                     ELAQIATVAWQAYAQGRKSPRTRKAGEGFADPNYELSIDWLEARAAIERAWHAHHDAH
                     GPARFLLINGASRSEHTCPGESSKSWRLTSLARNVIEESDKTSVEVLDLSRLASEYGR
                     RIFPCKTCFSTSPALCHWPCSCYPNYSLGQTQDWMNEIYPMWIAAHGILIVTPVNWFH
                     ASSPLKLMIDRLVCADGGNPDPTSTHGKDARVAKEIELDGWSYPRHLRGRLFACVVHG
                     DAEGAQAARRALCDWLSAMKLLPAGPESALDRYIGYWQPYATSHQQLDTDEAIQEEVR
                     NVARVLREAVEIDRAGKGIRAGTDLPVPRDK"
     gene            complement(2587..3042)
                     /locus_tag="VI195_05570"
     CDS             complement(2587..3042)
                     /locus_tag="VI195_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823888.1"
                     /translation="MDRATDSIEQHIYRERAVLRSNLEELEDRVRAVVDWRRHFRSSP
                     ALWLGCAFAGGLLIALGARPEEASRPLAYRREGEPFTRYPDHRQREISRAWRTIESAL
                     IGLAAGKLKEALARVIPGFHEQLARREGADYVHDFPGNNRSRHERHSQH"
     gene            complement(3048..3464)
                     /locus_tag="VI195_05575"
     CDS             complement(3048..3464)
                     /locus_tag="VI195_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6823889.1"
                     /translation="MHSRVQSLRAERPAPADDRSVAQVLADVIRNLQDILSAEIRLAQ
                     ARARMELRGYRPAAVLILIGVLGGLVSVFFLLLAVAAALALVIPWWAATLIVAVGTAV
                     LSTIMVRAGTLRVRSRAARTAKHLEEEMREGIGIKE"
     gene            complement(3470..3841)
                     /locus_tag="VI195_05580"
     CDS             complement(3470..3841)
                     /locus_tag="VI195_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823890.1"
                     /translation="MAQEYSPSGQDLGSTIDDGRIGALKAKAQDVGARAAQRADQARV
                     GAAAGLDNVASTLHEKGERVASAAHSAADAVSYGAEYLRENDVQTMLSDLMEVIRRNP
                     GPSLIGAAALGFMLGRALSRD"
     gene            complement(4341..5246)
                     /locus_tag="VI195_05585"
     CDS             complement(4341..5246)
                     /locus_tag="VI195_05585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY6823891.1"
                     /translation="MRAVLFTGYGGVDKLELREVPEPSAGPGEIKVRVAASSVNPIDW
                     KLRSGSYRQRSPLEFPAILGRDASGEVVATGPGAGAFRVGDKVLGLVNKAYAEYVVDK
                     ENAWAPLPAGLDLIDAAALPLVVLTGTQLIEEAVRPRAAEVVLVTGAVGSVGRAAVFA
                     ALARGAEVAAGVRAKQKDEARKLGVDVVDLDDAAELERLPRLDAIADTVNGDTLQKLI
                     GKVKPGGTIGSVLGEPAGAKERGLAVRAHLTHPDSTRLAALARSVAEGKLTIPIAKRI
                     PLADIREAHALAERGAGGKIVLRIR"
     gene            complement(5328..6047)
                     /locus_tag="VI195_05590"
     CDS             complement(5328..6047)
                     /locus_tag="VI195_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823892.1"
                     /translation="MAMDPAAKWQGQLILVTGGTGALGGAVARAFLEQGAHVAVTYRT
                     RQEFEDLRSAATAANARLSGHQVDVTNEAQMLGLTKALEAEFHRLDALVNAVGGYAGG
                     SKLWESDSALLERMLAMNLRSVFVSSRAVLPIMLRQGSGCIVNVAARSAVEQPGGAGA
                     YVASKAAALALMHSLAMDLKGTAVRVNSVLPNLIDTAANRRDMPRANFASWTKPEEIA
                     RVILFLCSADAQAVNGAAVPV"
     gene            complement(6067..>7384)
                     /locus_tag="VI195_05595"
     CDS             complement(6067..>7384)
                     /locus_tag="VI195_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823893.1"
                     /translation="EPSRHDANTAYVAVDRHKLDDIKPYVFETRDAGKSWKRIDAGLP
                     EGAFVHAVREDTVKRELLYAATETGVFVSFDTGGHWQSLQLNLPRSPIHDLVVKDDDL
                     VVATHGRSFWILTNVTPLREVASAAAASGVHLYKPAVAYRLYYPDQVDARPPSGQNPP
                     AGVMIDYYLTAAPKGPLTIEIVDASGGVVRHLTSVKPEQPEQPPEWPDQVRPLNTLPS
                     AAGMNRFIWNLRYDDPAQIPGAFYAGLPPRGPIALPGEYTVRLTYEGQTQTAPLVLKL
                     DPRVKGPAEGLQQKFALSLEVYHDQDALHRAVNDIRTFKAEMAAASKPSGGKSGSAAL
                     TAEGDSLVQRAAQIEAVLMQVNIKGSEANLNYAGMLNEQIYSFAELLDDADTAPNRPQ
                     VDTYAGLHSRLETELTAWADLKKTRIPAFCTRLRAAGQSSPMSSCQ"
BASE COUNT         1218 a         2453 c         2415 g         1298 t
ORIGIN      
        1 acaatgcatt gcagctgctg cgcatgcagc cctcggcggg gcagtcgcaa tgggctcagg
       61 aggtcataca gcgacagctc gcgtacctga cgcgcctggt ggacgatctg ctcgatgtgt
      121 cgcgcatcac gcgcggcaag atcgtgctca cccgcgagcc gctcgacgtc gcgaccctca
      181 tcacccgcgc gctcgagacg atagacccgc tgctacgcga gcgcgggcat gagctcacgc
      241 tgcagcttcc gcccgagccg ctgcgggtcg atggcgaccc gacgcgcctc acgcaagcgc
      301 tcggcaatgt gctcggtaac gcagcgaagt acaccgaacg tggcgggcac atccatttga
      361 tttgcacgcg gcaggggact gacgtcgaga tacgcgtgcg cgacaacggc atcggcatcc
      421 ccgcggagct gctgccgcga attttcgatc tgttcaccca actcgatcgc cgatcggatc
      481 aatccgccag cggcctcggc atcggactcg cactcgtacg ccggctcgtg gaaatgcacg
      541 ggggcagcat cagcgcagtg agcgctggag ctggtgcggg cagcgagttc gtcatttctc
      601 tgccgctgct ctccgatgag ctcgcgcgtg agcacgatgc ggtcagggag caggcgcgcg
      661 aggtgccggc gctgccccgg cgcatcctgc tggcggacga caaccccgac gcactcgaga
      721 gccttgcgac ggtgctgcgg ctgcggggtc acgaggtgta cagcgcccgt aacggtgccg
      781 ctgcactgga gacggccgcc cggcacgtgc cggaggtggc gctgctcgat atcggcatgc
      841 cgctcgtcga cggctacgag gtcgcgcggc gcattcgcgc acaggagtgg ggtaagggaa
      901 tcacgttggt cgcgctgacc ggctggggac aggagtccga tcgccgccgc tcacaggagg
      961 cgggtttcga cgcgcatctg gtaaagccac tcgacctcga caagctcacc gctgtcctgg
     1021 cgcagctgcc ggaacacgtc ccgcatgtag gagatctcct acccgcggtg tagtcacgcg
     1081 ctgcgagaca gattcagccg tgcgagccga gcatgaggcc accgcagcgc gtcaggttca
     1141 ggtggtcgaa tgaacgaaga aatggaaccc gaagtcctca gctaccgaga attcaggaat
     1201 cgcgaagcgc tactcgcctg ggagcgacgt cgtcgggcct acgaattgca aatcggcgag
     1261 cgcagtgagc ggcgcgggag ggtgttcatg cccttgcctc gtcgtgtggc gggtgccgag
     1321 ccgcggcccc agcgctgagc ggatcgcggt tacttgtcgc gtggcaccgg cagatcggtg
     1381 cccgccctga tgcccttgcc ggcgcgatcg atctcgaccg cctcgcgcag cactcgcgcg
     1441 acgttacgca cctcctcctg gatggcttca tcggtgtcga gctgctggtg gctcgtggcg
     1501 tagggctgcc agtagccgat ataacggtcg agcgcgctct cgggtccggc gggaagcagt
     1561 ttcatcgcgg acagccagtc acacagcgcg cgtcgtgccg cctgggcgcc ctccgcgtcg
     1621 ccatggacga cgcaggcgaa caggcgcccg cgcaggtggc ggggatagct ccacccatcg
     1681 agctcgattt ccttggcaac gcgcgcatcc ttgccatgcg tgctggtggg atccggatta
     1741 ccgccatcgg cacagacgag ccggtcgatc atcagcttga gcggtgacga tgcgtgaaac
     1801 cagttcaccg gagttacgat caagatgccg tgcgcggcga tccacatcgg gtagatctcg
     1861 ttcatccagt cctgcgtctg tccgaggcta tagttgggat agcaggagca gggccagtga
     1921 cacaatgccg gtgaggtgga aaagcaggtc ttgcagggga agatgcggcg accgtattcc
     1981 gacgcgagac ggctgagatc gagcacctcg acactggtct tatcgctctc ctcgatcacg
     2041 ttccgggcga gactcgtgag ccgccaggat ttcgagctct ccccgggaca ggtgtgctca
     2101 ctgcggctcg cgccgttgat cagcaggaag cgcgccggcc cgtgcgcatc atggtgcgcg
     2161 tgccaggcac gctcgatcgc cgctcgcgcc tcgagccagt cgatcgacag ctcatagttc
     2221 ggatcggcga agccctctcc tgccttgcgc gtccgcggac tcttgcgccc ctgagcgtag
     2281 gcctgccacg cgactgtcgc gatctgcgca agctcgctgg caagcctgtc gaaggccggg
     2341 tcgttgaatt gggccaggaa tcgccgcttg aactcccgtt ctccgagggc tgggtcgggc
     2401 atctcgcggc gcggcgcagg tgcctgccgt ggggtgttgg tgcccatgaa tcgcgcagcg
     2461 ccgcccgccc gcgcggcagc cgccgctccc tcctagtggt gggagcacat atacagcagc
     2521 aggatgatgg gtatcggcac tcccagaagc cacagaagaa ttccacgtcc catgtgattg
     2581 cctcgcttag tgttgactat gtctctcgtg tcgactgcga ttgttcccgg ggaaatcgtg
     2641 cacgtaatca gcgccttcgc gccgcgcaag ttgttcatgg aacccgggta ttacccgggc
     2701 gagcgcttcc ttgagtttgc ccgctgcgag tccgatcagc gcgctctcga tggtgcgcca
     2761 ggcgcgtgag atctcccgct gccggtgatc ggggtagcgg gtgaacggct cgccctcgcg
     2821 ccggtacgcg agcggtcgtg aggcttcctc cggcctggcc ccgagcgcga tcagcagccc
     2881 gccggcgaac gcgcagccca gccacagggc cggactgctg cggaaatgcc gccgccagtc
     2941 gaccaccgcc ctgacgcggt cctcgagctc ctcgaggttc gagcgcagca ccgcccgctc
     3001 gcgataaatg tgctgctcta tgctgtcggt cgcccggtcc atggcgccta ctcctttata
     3061 ccgatacctt cccgcatctc ttcttctagg tgtttggcgg tcctggctgc acggctgcgc
     3121 acgcggagag tgccggcgcg caccatgatc gtggacaaca ccgccgtgcc gaccgccacg
     3181 atcagtgtgg ccgcccacca agggatgacg agagctaatg ctgccgccac cgccagcagc
     3241 agaaagaaga cactcacgag tccgccgagt accccgatca ggatcagtac ggctgcgggg
     3301 cgatagccgc gcagctccat gcgcgcccgt gcctgggcga ggcggatttc ggcgctgaga
     3361 atgtcctgga ggttcctgat gacatccgcc agcacctgcg caacggagcg atcgtccgcg
     3421 ggcgcaggcc gctccgcgcg caaggactgc actcgtgagt gcatgcgctt taatcgcggc
     3481 tgagcgcgcg gcccagcatg aatcccaaag ccgcagcgcc gatcagcgag ggcccgggat
     3541 tgcgccggat cacctccatg agatcactca acatggtctg cacgtcgttt tcgcgcagat
     3601 actcggcgcc ataagacacc gcatccgccg cactgtgcgc ggcgctcgct acgcgttcgc
     3661 ctttctcgtg caacgtgctg gccacgttgt ccagaccagc ggccgccccg acacgcgcct
     3721 gatcggctcg ctgtgcggcc ctggcaccga cgtcctgagc cttggccttg agcgctccga
     3781 tcctgccgtc gtcgatggtg gatccgaggt cctgacctga aggcgaatat tcctgtgcca
     3841 tggcggtact cctttcttgg gcggtgagat gtaacatgca tgcgccgctg ctttccgtaa
     3901 gaagaacgct tgagctgcag tcagacgcta gcggtcttac ccgcatccgc ccaactgtcc
     3961 gacgctcctc gcgggccgga tgctacgcgt aggtacaagc tgacgctgct tcagcggtgg
     4021 acccgaccga cacggcgagc gctccgtagc accctgatcc cgatggccaa ggcaaacaaa
     4081 gaaggcacgg cacagatcgg caccgaaatg cgcgagtcat tcgatgccac tctggctctc
     4141 aagcccgcgg agatcgaggc actcattgcg cggctgtgcg cgtgcggcac ccgccgcgtc
     4201 aagtcaatcg gacccagccg aatggctacg gagtgcgggc gcagaatacg cgcagagtgg
     4261 tgccgtttgg gcgatcgggg agccagcact ctgcggctcg tcgataccaa atttggttga
     4321 gcccctcgcg ggaacgccgc tcaccggatt cgcagcacga tcttcccgcc cgcgccccgc
     4381 tcggctaggg cgtgggcctc gcggatatct gccagcggta tgcgtttggc gatgggtatc
     4441 gtgagttttc cttccgccac ggaccgtgcc agcgccgcga gccgcgtgga gtcggggtgt
     4501 gtgaggtgcg cacgcaccgc cagtccccgc tccttcgcac ccgctggctc cccgagcaca
     4561 ctgccgatcg tgcctccggg tttgaccttg ccgatcagtt tctgcagcgt atcgccgttc
     4621 accgtgtcgg cgatcgcgtc gagccgggga agacgctcga gctccgctgc atcgtccagg
     4681 tccacgacat cgacaccgag cttgcgagcc tcgtccttct gcttggcgcg aactcccgcg
     4741 gcgacctcgg ctccgcgcgc tagcgcagca aacaccgccg cccggccaac actgcccacc
     4801 gcgccagtca cgagtacgac ctccgccgca cgcggtctca cggcctcctc gatcagctga
     4861 gtgccggtga gcaccacgag tggcagcgcc gccgcatcga tgaggtcgag tcccgcaggc
     4921 agcggcgccc aggcattctc cttatccacg acatattcgg cgtaggcctt gttgacgagg
     4981 ccgaggacct tgtcgccaac tcggaatgcg cccgctccgg gtcccgtggc gacgacctcg
     5041 ccgctggcat cccggccgag tatcgccggg aactcgagcg gcgagcgttg ccggtaagag
     5101 ccgctgcgca gcttccaatc gatcggattt acgctcgagg cggcgacgcg caccttgatc
     5161 tcgccaggac ccgcactggg ctcgggcacc tcgcgcagct cgagcttatc gactccgccg
     5221 taaccggtga atagcacggc acgcatgacc gccggaatgt gcacgcctgc cggctgcgga
     5281 gtcgatcggc ttttcgcagc aactcctgta agggatggcc tactccgtca gacgggtacc
     5341 gcggcgccgt tgactgcctg cgcatccgct gaacacagaa acagaatgac gcgcgcgatt
     5401 tcctccggct tcgtccagct ggcgaagttg gcgcgcggca tgtcgcgccg gttggctgca
     5461 gtgtcgatga ggttcggcag caccgaattg acgcgaacgg cggtgccctt caggtccatc
     5521 gccaaggagt gcatcagagc gagtgccgcc gctttggagg cgacatacgc tcctgcgccg
     5581 cccggctgct ccacggccga gcgggccgcg acattcacga tgcagccgct gccctgcctg
     5641 agcatgatcg gcagcacggc acgtgagctc acgaagaccg agcggagatt catcgccagc
     5701 atgcgctcga gcaacgcgct gtcgctctcc cacagcttcg atccaccggc gtacccgccg
     5761 acggcattca cgagcgcatc gagccgatga aactccgcct cgagcgcctt ggtgaggccg
     5821 agcatctgcg cctcgttggt tacatcgacc tggtggccgc tcagccgtgc gttggcggca
     5881 gtcgcggcgc tgcgcagatc ctcgaattcc tgccgcgtgc ggtaggtcac cgccacgtgt
     5941 gcgccctgct cgaggaaggc gcgcgcgacc gcgccgccga gcgcgccggt gccgccggtt
     6001 accaggatga gctgtccctg ccatttcgcc gccggatcca tggccatacg atgccacctc
     6061 tactgtctac tgacagctgc tcatcgggct ggactgaccg gcagcgcgca gccgggtgca
     6121 gaaggcgggg atgcgcgttt tcttcaggtc cgcccaggcg gtgagctcgg tctcgagccg
     6181 cgagtgcagg ccggcatagg tgtctacctg cggcctgttc ggcgcggtat cggcatcatc
     6241 cagcagctcc gcaaaggaat agatctgctc gttcagcatg ccggcatagt tgaggttggc
     6301 ctcggatccc ttgatgttga cctgcatcag aaccgcttcg atctgtgcag ccctctgcac
     6361 gagcgagtcg ccctcggccg tgagcgcggc actaccggac ttgccgccgg acggcttcga
     6421 tgccgccgcc atctcggctt tgaaggtgcg gatgtcgttc acggcgcgat gcagcgcatc
     6481 ctggtcgtgg tagacctcca gcgacagcgc gaatttctgc tgcagacctt cggccggacc
     6541 tttcacgcgt ggatcgagct tgaggacaag cggcgccgtc tgcgtctgcc cttcgtaggt
     6601 cagcctgact gtgtattcac ccggcagcgc tatcggaccg cgcggcggca ggcctgcgta
     6661 gaaggctccc gggatctgcg ccggatcgtc gtagcgcagg ttccagatga agcggttcat
     6721 gccggccgcc gagggtagcg tgttgagagg acgtacctgg tcgggccact cgggcggctg
     6781 ctcgggctgc tccggcttga cgctcgtcag gtggcgcacg actcccccgc tcgcatcgac
     6841 gatctcgatg gtgagcggtc ccttaggggc ggctgtcagg tagtagtcga tcatcacgcc
     6901 ggccggaggg ttctgaccag aggggggccg ggcgtcgacc tgatcggggt agtagagacg
     6961 ataggcgacc gcgggcttgt agagatgcac gccggaagcc gccgccgcgg aggcgacctc
     7021 acgaagcggt gtcacattgg tgagaatcca gaacgagcgc ccgtgtgtgg cgaccaccag
     7081 atcatcgtcc ttgaccacga gatcgtgaat cggactgcgt ggcaggttga gctgcagact
     7141 ctgccagtgc ccgccggtgt cgaaggacac gaacaccccg gtctcggtgg cggcgtacag
     7201 cagctcacgc ttgaccgtat cctcgcgcac cgcgtgcacg aaggcgccct cgggcagacc
     7261 cgcgtcgatg cgcttccagc tcttgccggc atcgcgagtc tcgaagacat agggcttgat
     7321 gtcatcgagc ttgtgacggt ccacggctac gtaggcggtg ttggcatcgt ggcgcgaggg
     7381 ctcg
//
LOCUS       DATDMP010000322         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1717774, whole genome shotgun sequence.
ACCESSION   DATDMP010000322 DATDMP010000000
VERSION     DATDMP010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717774"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VI195_05600"
     CDS             complement(<1..545)
                     /locus_tag="VI195_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823894.1"
                     /translation="MALRDAQARQAAKGYTLAIALGLLPVRGIAADTPDIPGTANTPN
                     TLSLGVDVGLGATDNIFEVQSPKTSEVMPTAGLDLALHRQGTRLDADVTGVFAYYDYL
                     RNSYSNTVFGRLDGSAKLALVPERFIWVLQESFGQAQLNILAPVTPANQENVNVVSTG
                     PEFALHLGEQGFIHLGARYADV"
     gene            complement(508..1404)
                     /locus_tag="VI195_05605"
     CDS             complement(508..1404)
                     /locus_tag="VI195_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6823895.1"
                     /translation="MSLIESAIEKMRRSGYAADLRRVRAAPRADAPAGAGASEHPPLQ
                     PLAPPTTRISLDQESLRSAGYLPEATRSALFADQYRHIKRPLIEKVRSDPGAPQLRLI
                     LVTSALAGDGKTFTSINLALSMARERDTSVLLIDADMRNPSASRILGVAGERGLLDVL
                     SDESLDVGSLIQGTSVPGLDILPAGAPADHASEHLASARMAHIAAQICANPRYLVIID
                     CAPVLLSSEAGALAQLVGQIVLVARAGKTPRQALLDAIARFDGSKLSGIVLNDSKADP
                     GGYHYYGYGNYGTQGRASAAGS"
     gene            complement(1401..>2358)
                     /locus_tag="VI195_05610"
     CDS             complement(1401..>2358)
                     /locus_tag="VI195_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY6823896.1"
                     /translation="RRAELSRQLHGDVAIAGAAPMSGGPGGLRPSDTVSRIAETQARL
                     DELLLKYTDKHPDVIAARETLEELKARRAAEIAGLRRGDPNAAATSGASSNPVYQGAA
                     LALTQADVEIAALRAELQQHESKVAELRRLLNTAPQVEAQYAQLNRDYDVNKTQYTAL
                     LQSYQKARIGEKADTAGSVRFEVVQPPTADFVPVWPPRLRLLAVIFVAAIGCGAALAY
                     ALHWLWPVVGSARSLEQLTQLPVLGVVSSGFPGADRRAARRDLLGLSTAFACLVLAFA
                     VVLVLNQVGVRLSIGTLAHEAHAMISPVEIRLGSALTHGAQT"
BASE COUNT          375 a          795 c          788 g          400 t
ORIGIN      
        1 acatcggcat accgggcgcc gagatggatg aatccctgct cgccgagatg caacgcaaat
       61 tcggggccgg tcgagacgac gttgacgttc tcctggttgg cgggtgtcac gggcgcgagt
      121 atgttgagct gcgcctggcc gaagctctcc tgcagaaccc agatgaatct ctcaggcacg
      181 agcgccagct tggccgagcc atcgaggcgg ccgaagaccg tattcgagta gctgttccgc
      241 aggtagtcgt aataggcgaa cacgccggtc acatctgcgt cgagtctcgt tccctgccgg
      301 tgcagcgcga gatcgagccc ggccgtcggc atgacctcgg aggtcttcgg gctttgcacc
      361 tcgaagatgt tgtcggtggc gcccaggccg acatcgactc cgaggctgag tgtgttcggc
      421 gtgttcgcag tgcccggaat gtccggtgtg tccgcggcga tgccgcgcac gggcaagagc
      481 ccgagagcga tggccagcgt gtacccttta gctgcctgcc gcgcttgcgc gtccctgagt
      541 gccatagttt ccgtacccgt aatagtgata ccccccggga tctgccttgc tgtcattgag
      601 aacgatgccc gacagcttgc tgccatcgaa ccgtgcaatt gcatcgagga gcgcctgccg
      661 gggcgtcttg cctgcccggg cgacgaggac tatttgtccg acgagctgcg ccagcgcacc
      721 tgcctcgctc gacagcagca cgggcgcgca atcgattatg accaggtagc gcggattggc
      781 gcagatctgc gcggcgatgt gcgccatgcg ggcactcgcc aggtgctccg acgcatgatc
      841 cgccggcgcg cccgccggca gaatatcgag ccccggcacg ctcgtcccct gtatcagcga
      901 gccgacgtcc agcgactcgt cgctcaagac atccagcaac ccgcgctcgc cggcgacgcc
      961 gaggattcga ctggcactcg ggttgcgcat gtctgcgtcg atgaggagca ccgaggtgtc
     1021 ccgctcccgc gccatgctga gcgcgagatt gatgctggtg aaggtcttgc cgtctccggc
     1081 aagagcgctg gtgaccagaa tgagccgcag ctgcggtgcg ccgggatcgg accgtacttt
     1141 ctcgatcagg ggccgcttta tgtggcggta ctgatccgcg aacagcgcgc tgcgcgtcgc
     1201 ctcggggagg tatccggcgc tccgcaggga ctcctggtcg agcgaaatgc gcgtcgtcgg
     1261 cggggccagc ggctggagcg gcgggtgttc cgaagctccg gctcctgccg gcgcgtcggc
     1321 ccggggagcc gccctgactc gcctgagatc ggcggcgtaa ccgctccgcc gcatcttctc
     1381 gatggccgat tcgatcaggc tcacgtttgt gccccatgcg tgagtgcgct gcccagtcgg
     1441 atctcgaccg gacttatcat cgcgtgcgcc tcatgggcga gtgtgccgat gctgaggcga
     1501 acgccgacct gattgagcac cagcaccacg gcaaaagcca ggacgagaca ggcaaaagct
     1561 gtcgagagcc ccagcagatc gcgacgggcg gcacgtcggt ccgccccggg gaagcccgag
     1621 ctcacgacgc cgagcaccgg cagctgcgtc agctgctcga gggagcgggc cgaacccacc
     1681 accggccaca accagtgcag ggcgtacgcg agcgcagcac cgcagccgat ggcagccacg
     1741 aatatcaccg ctagtaaccg caggcgcggc ggccataccg gaacaaaatc ggctgtcggc
     1801 ggctgcacga cctcgaagcg cacggagccc gcggtgtcgg ccttctcccc gatgcgcgcc
     1861 ttctggtagc tctgcagcag cgccgtgtac tgcgtcttgt tgacatcgta gtcgcggttg
     1921 agctgggcgt actgcgcttc gacctgcggc gcggtattca gcagccggcg cagctcggcg
     1981 accttgctct catgctgctg cagctcggcg cgcagcgcgg cgatctccac gtccgcctga
     2041 gtgagcgcca gagccgcccc ctggtagacc gggttgctgc tcgcgccgct ggtggctgcc
     2101 gcattcggat caccccgcct gagacccgcg atctcggcgg cgcgccgcgc cttgagctcc
     2161 tcgagtgtct cccgcgcagc gatcacgtcc gggtgcttgt cggtgtactt gagcagcagc
     2221 tcatcgagac gcgcctgcgt ctcggcaatg cgcgacacgg tgtcggacgg ccgcaatccc
     2281 cccggcccgc cggacatcgg ggcggcgccc gcgatcgcca cgtcgccgtg caactgccgg
     2341 ctcaactcgg cacggcgt
//
LOCUS       DATDMP010000323         4583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_495119, whole genome shotgun sequence.
ACCESSION   DATDMP010000323 DATDMP010000000
VERSION     DATDMP010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495119"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VI195_05615"
     CDS             <1..584
                     /locus_tag="VI195_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823897.1"
                     /translation="RFATPAVAPELLAALAQVEGAGNPLVHTYWRWSWAVKPFELYRP
                     ASSAVGMYQITDGTFAEARHYCIHHHALAQEGAWNDWRSCWFNDLYLRVLPRDAIELT
                     AAYLDLKVNAILAQSPRRTPSALQRQHLAAVVHLCGAGAAASYALRGFRFAAGERCGT
                     HDPREYLLRVDEMRAVFTRLAQTDAQASALSAQ"
     gene            complement(578..1060)
                     /gene="slyD"
                     /locus_tag="VI195_05620"
     CDS             complement(578..1060)
                     /gene="slyD"
                     /locus_tag="VI195_05620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6823898.1"
                     /translation="MSISQDQVVSIHYTLRDDAGEVIDRSAAGEPLAYLHGHGQLIPG
                     LERELTGRDAGDRLQVKILPAEGYGEYDPELVQRVPRRALKGIGEVRVGMRLQAQTAE
                     GLRAVTVTQLTGDMVTLDGNHPLAGKNLHFEVEVAAVRAATEQELAHGHVHGAGGHHH
                     "
     gene            1194..3392
                     /locus_tag="VI195_05625"
     CDS             1194..3392
                     /locus_tag="VI195_05625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY6823899.1"
                     /translation="MRIVCLGALVAVFATTVLAAGDARAGGHPRTPAMADNPFAAPST
                     LPFQLPPFDHIHDSNFRPAFDAGMGEQLRQVEAIAHNSAAPTFANTIVALERSGRTLE
                     QVETTFSNLNACNTDPEMQRIDTDMAPRLTAQRDAIHLNAALWARVDALYNERESLHL
                     DPESLQLLTRYHTIFVRAGARLSADQQARLKDLNKQISTLTTRFKQNVLKATADGAVV
                     VDDAAQLDGLTAEQVGAAAAAAAGRGLSGKWLLALQNTTNQPVLARLTDRALRERVYK
                     ASTARGLEGATDNTAVISELVRLRAERAALLGYPDHAAYELADESAGTPEAVRGMIGQ
                     VAPAALARARAEAADMQKVIDAEAAAAGRASFALQPWDWSYYAEKVRKARYDFDQAAV
                     APYFELDHVLKDGVFYAAHQLYGLTFRERHDLPVYQKDVRVFEVFDADGKPLALFLAD
                     YFARDNKQGGAWMTAYVNQSRLLQQKPVVANHLNISKPQPGQPVLLTFDEVTTMFHEF
                     GHAVHGLLSDVHYPLLSGTAVPRDFVEYPSQYNEMWAREPQVVAHYARHYQSGAPMPP
                     ELLAKVLAAQNFNQGYATLEYLEAALLDQSWHQITVAQAPSAAQVPAFERQALEASGL
                     DYAPVPPRYHSTYFAHIFEGGYSAGYYAYLWSEVLARDTGQWFHSHGGLTRANGEYLR
                     THVLARGRSEDPQQLFREFYGRPPEIGPLLEYRGLTGAGGGTAVGAANGS"
     gene            complement(3415..4395)
                     /locus_tag="VI195_05630"
     CDS             complement(3415..4395)
                     /locus_tag="VI195_05630"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6823900.1"
                     /translation="MPEFDFAILGAGAIGSILGAHLARAGHSVAMLARGARAAHLERH
                     GLAIRGLVEFTTPVRTLRDPQALRSAGALIVATKTPGTAAALEPLRAAQFEVTLSIQN
                     GPLKNELLSAAFGAERVLGALADTSGELLPGGEVLFTRNVGIFLGELSGGESARARRL
                     AQTLDSAGVRAMATPDILSLEWSKFCGWVGLMALSVATRAPTWMYLSDPDSALLITRL
                     VREMCTLARALGIPLSDRAVLPAARLCAAPESEAVAFVLQLGEEYRRSSPGHRMSSLQ
                     DVEASRPLEVHETLGYASAKARELGLDLPLLAAFTRLLAAIDRTRRSALI"
BASE COUNT          734 a         1605 c         1548 g          696 t
ORIGIN      
        1 gccgcttcgc cacgcccgcc gtcgcgccgg agctgctggc ggcgctcgcc caggtcgagg
       61 gcgcaggcaa cccgctggta cacacctact ggcgctggtc gtgggcggtg aagcccttcg
      121 agctctaccg cccggcctcg agcgcggtcg gcatgtacca gatcaccgac ggcactttcg
      181 ccgaggcgcg gcattactgc attcaccacc atgcgcttgc ccaggagggt gcgtggaacg
      241 actggcgctc gtgctggttc aacgacctgt acctgcgcgt gctgccgcgc gatgcgatcg
      301 agctcactgc ggcctatctc gatctgaagg tgaacgccat actcgcgcag agtccgcgcc
      361 gcaccccgag cgcactgcag agacagcacc tggcggcggt ggtgcacctg tgtggcgccg
      421 gtgcggcggc gagctacgcg ctgcgcggct tccgcttcgc ggcgggtgag cgctgcggca
      481 cccacgaccc ccgggagtat ctgctgcgcg tcgatgagat gcgcgcagtg ttcacacgct
      541 tggcacaaac ggatgcgcaa gcgagcgcgc tgagcgctca atgatgatgg ccgcccgctc
      601 cgtgcacgtg cccgtgcgcg agctcctgct cggtggcggc acgcacggcc gcgacctcga
      661 cctcgaagtg caggttcttg ccggcgagcg gatggttgcc atcgagcgtc accatgtcgc
      721 cagtgagctg ggtgaccgtg acggcgcgca ggccctccgc cgtctgggcc tgcaggcgca
      781 tgcctacccg cacctcgcca atgcccttga gcgcgcgccg cggcacacgc tgcacgagct
      841 ccgggtcgta ctccccgtag ccctcggccg ggaggatctt cacctgcaat cgatcgccgg
      901 catcgcgtcc cgtcagctca cgctcgagtc ccgggatcag ctgtccgtgc ccatgcaggt
      961 aggcgagcgg ctcgcccgcg gcagagcggt cgatcacctc acccgcatcg tcgcgcaggg
     1021 tgtagtggat gctgacgacc tggtcctggc tgatgctcat gtgggctccg atcggcgggg
     1081 gaacgcgcgc ctgccgcacc gggcgggtac agtgcacgcg ccgagcgggc gtggaggata
     1141 ccattgcgct cgcccgcagc cctcgggcaa ctggtcaacg cagagggatc cgcatgagaa
     1201 tcgtttgcct gggcgcatta gtcgccgtgt tcgccacgac cgtgctcgcc gccggggatg
     1261 cccgcgcggg cgggcaccca aggactcctg caatggccga caacccgttc gctgcgccaa
     1321 gtacgctccc gttccagctg ccgcccttcg atcacattca cgacagcaac ttccgcccgg
     1381 cgttcgatgc gggtatgggc gagcagctgc gccaggtcga ggccatcgcg cacaactccg
     1441 ccgcgccaac cttcgcgaac acgatcgtgg cactcgagcg ttccggcagg acgctcgagc
     1501 aggtcgagac caccttctcc aatctcaatg cctgcaacac cgacccggag atgcagcgca
     1561 tcgataccga catggcaccg cgcctgacgg cgcagcgcga tgcgatccac ctgaatgcgg
     1621 cactgtgggc acgtgtcgat gcgctctaca acgagcgcga gagtctgcat ctcgatcccg
     1681 aatcgctgca gctgctgacg cgctaccaca ccatcttcgt gcgtgccggg gcacgtctca
     1741 gcgcagatca gcaggcgcgt ctgaaggatc tcaacaagca gatctctacc ctcaccacac
     1801 gcttcaagca gaacgtgctc aaggcgaccg ccgatggcgc cgtggtcgtc gatgatgcgg
     1861 cgcagctcga cggactgacg gccgagcagg tgggcgcggc cgccgccgct gcggcgggac
     1921 gcggactcag cggcaaatgg ttgctcgcgc tgcagaacac aaccaaccag ccggtgctcg
     1981 cgcgactcac cgaccgggcg ctgcgcgagc gcgtctataa agcatcgacc gcccgcggtc
     2041 tcgagggtgc gaccgacaac accgcggtga tctccgagct cgtgcggctg cgtgccgagc
     2101 gcgccgcgct gctcggttat cccgatcatg ctgcctatga gctcgcggac gagtccgccg
     2161 gcacaccgga ggcggtgcgc ggcatgatcg gtcaggtggc gccagcggcg ctcgcgcgcg
     2221 cccgtgcgga ggcggccgat atgcagaagg tgatcgatgc cgaggcggcc gccgcgggca
     2281 gggcttcctt tgctctgcag ccttgggact ggtcctacta tgccgaaaag gtgcgcaagg
     2341 cgcgctacga cttcgatcag gccgccgtcg ccccgtactt cgagctcgac cacgtactga
     2401 aagacggcgt cttctacgcg gcccaccagc tctacggact caccttccgc gagcggcacg
     2461 acctgcccgt gtaccagaag gacgtgcgcg tcttcgaagt gttcgatgcc gatggcaagc
     2521 cgctcgcgct gttccttgcc gactatttcg cgcgcgacaa caagcagggc ggggcgtgga
     2581 tgaccgcgta cgtgaaccag tcgcgtctgc tgcagcagaa accggtggtg gccaaccacc
     2641 tcaacatttc caagccccag cccgggcagc cggtgttgct caccttcgat gaggtgacca
     2701 ccatgttcca tgagttcggc cacgcagtgc acggactgct gtcggacgtg cactacccgt
     2761 tgctctccgg caccgcggtg ccgcgcgact tcgtcgagta cccttcccag tacaacgaga
     2821 tgtgggcgcg tgaaccgcag gtggtggcgc actacgcgcg tcactaccag agcggcgcgc
     2881 cgatgcctcc ggagctgctc gccaaggtgc tggcggcgca gaatttcaac cagggctacg
     2941 ccaccctcga gtacctcgag gcggcgctgc tcgatcagtc ctggcatcag atcacggtcg
     3001 ctcaggcacc gagcgcggcg caggtgccgg cattcgagcg gcaggcgctc gaggcgagcg
     3061 gactcgacta cgcgccggtg cctccgcgct atcactccac ttactttgcg cacatcttcg
     3121 aaggcggcta ttcggcgggc tactacgcct acctctggag cgaggtgctg gcacgcgaca
     3181 ccgggcagtg gttccacagt cacggcggac tgacgcgcgc caacggcgag tatctgcgca
     3241 cgcacgtgct cgcgcgcggc cggagcgagg atccgcagca gctgttccgc gagttctatg
     3301 ggcgcccgcc cgaaatcggt ccgctgctcg agtaccgcgg cctcaccggc gcgggcggcg
     3361 gcacggccgt cggggccgcc aacggcagct gagcgcccca ggccgccggc gcggctagat
     3421 gagggcgctg cggcgcgtgc ggtcgatcgc tgcgagcagg cgggtgaaag cggcgagcaa
     3481 cggcagatcg agcccgagct cacgcgcctt ggccgaggca tagccgagcg tttcgtgcac
     3541 ttcgagcgga cggctcgcct ccacatcctg cagcgaggac atgcggtggc cgggagagct
     3601 gcggcggtac tcctccccga gctgcaggac gaacgccacg gcttcgctct ccggagccgc
     3661 gcacaagcgc gcggccggca gcactgcgcg atcggagagc gggataccga gcgcgcgtgc
     3721 cagcgtgcac atctcgcgca ccagccgcgt gatgagcagc gcggagtcgg ggtcggacag
     3781 gtacatccag gtgggcgccc gagtcgcaac cgagagtgcc atgagtccca cccagccgca
     3841 gaacttggac cactccaggc tcaggatgtc cggggtcgcc atggcgcgca cgcccgcgga
     3901 gtcgagcgtc tgggcgaggc gccgtgcgcg cgcgctttcg ccaccgctca gctcgccgag
     3961 gaaaatgccc acgttacggg tgaacagcac ctcaccgccc ggcagcagct cgccgctcgt
     4021 gtccgccagc gccccgagca cgcgctcggc tccgaaggcg gcgctgagca gctcgttctt
     4081 cagcggcccg ttctgaatcg acagggtcac ctcgaactgc gccgcgcgca gcggctcgag
     4141 agccgcagcc gtgcccgggg tcttggtggc gacgatgagc gcgccggcac tgcgcaacgc
     4201 ctgtgggtcg cgcagcgtgc gcaccggggt cgtgaattcc accaggccgc ggatcgcgag
     4261 gccgtgccgc tcgaggtgcg cggcgcgcgc gccgcgcgcc agcatggcga cactgtgtcc
     4321 ggcgcgcgcg aggtgggcgc cgaggatcga gccgatggcg cccgctccca gtatcgcgaa
     4381 atcaaactcg ggcacggcgc tgccccgggg cggcgtcaga gcgcctcggc gatgtcctgc
     4441 atcagcccgt tatcgcgcgg cttggtttcc ggcggatagc gctgcagcac ccggccatcg
     4501 cggccgatga ggaacttctc gaagttccag ctgatctcgc ccggaatacc cccggcagcg
     4561 gcagtgagga attggtacac ggc
//
LOCUS       DATDMP010000324         5197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_495514, whole genome shotgun sequence.
ACCESSION   DATDMP010000324 DATDMP010000000
VERSION     DATDMP010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495514"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..2026
                     /locus_tag="VI195_05635"
     CDS             164..2026
                     /locus_tag="VI195_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6823901.1"
                     /translation="MTEKPSLTTAGRLSHKVKPADRGKLKIGDDWNAIRIIALSQSNP
                     LKAIAEFVENSIDAHSKTITITRGREHREHYLLIKDDGDGVPRDEGGRPDFKYVATHI
                     CDSIKRRLKAEGEGKSVQGEFGIGLLSFWTVGNSLTMISTGADQRSYEMTMSKGDSGY
                     AVRPRRVLIAERGTELRISPLLDGIRNLSGEKIQWYLASELRDRIRKAKVRVTVIDRL
                     ARKQYEVEPRVYEGRLLHQLPAVTTTYGEAYAELYLTEPDENCRVALSRAGTRVIEDL
                     TTLPGLDHSPWTSRYLQGLIDAPFLNLTPSTRSGILHDERYAALAQALPPLEQQLNAL
                     IEAQQRAEEEEASRESLRAIQRAFREALLALPPEEYDWFEIQARARSDSGPETSGVGN
                     GTFTAAEAQSAELGVAEPELHPGEQRQFFEYAGPLFSVVISPASSTTPLNERKKFRAL
                     PRDRSRRRVVEDLQFSWEIVEGGGSLSDTRNQEIEYLAPANPGLARLKVSVTQREITC
                     VAEALITITATLETSVGSTVVTARGLPGYTFERAAGELWRSRFDAARNLIIVNNGHRD
                     FVFARRNRALQLRYLVRLYVKELVLRNFAGAASEQLLERMIELTLYAEEKLKSG"
     gene            complement(2099..2183)
                     /locus_tag="VI195_05640"
     tRNA            complement(2099..2183)
                     /locus_tag="VI195_05640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2147..2149),aa:Leu,seq:cag)
     gene            2363..4324
                     /gene="rnr"
                     /locus_tag="VI195_05645"
     CDS             2363..4324
                     /gene="rnr"
                     /locus_tag="VI195_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY6823902.1"
                     /translation="MASSRARHTASSLRPGTIVEGTVSANRAGYGFLRVEGAGESVFL
                     PPPQMRGVMHGDRLRVRVSRDGSARWSGTVEQVISRGVNSFLGTLEIQGRSAWVNAAD
                     RRLQLRCAVAPPDLHGARHGDWAIARITRHAGSASAAQARIEKVLDPDRPVELATESA
                     IARFDLPHEFPAETLREARVFGEHVDPREAAARVDLRELPLVTIDGEDARDFDDAVYA
                     EPHAGGFRLIVAIADVSHYVRPGSALDAQAQQRGTSVYFPTRVLPMLPTALSDHLCSL
                     APHVERLCFAADMIVSRSGALKSARFYPAVMRSAARLTYTLANQALFEGRPAARVKLG
                     ALVERLMVLVDVYRALYRARARRGALDFDAAEAEFVIDAGEHVRAIELRVRNDAHRLI
                     EECMILANVAVARELETSHTPTLYRVHGVPEDEKLERLASTLASLGIDARIPKTVTTR
                     DLQAIARRVSDASERTFIESLVVRAMPQAIYQPTNIGHFGLALTHYAHFTSPIRRYPD
                     LIVHRTLKARIKASGGAGRRYAVGELGPLGESTSRLEKRADEADRYVSGFLKCTYLKE
                     RIGQTFQGLITTVVEFGCFVQILDVAVDGLLHINNLHDDLYVMEEDGHAWRGRRSGRR
                     LRTGAHVRVIVTAVNPIEGLIDLALAEDG"
     gene            4356..5099
                     /gene="rlmB"
                     /locus_tag="VI195_05650"
     CDS             4356..5099
                     /gene="rlmB"
                     /locus_tag="VI195_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY6823903.1"
                     /translation="MKQSETIYGMHAVRVMLERHAQRVLNVRLAAQRDDPRAREIEQL
                     AREAERPVQRIDMHALRQQLGAVAHQGVAAEITPLPPWSEDELLEALQSARAPLLLAL
                     DGVQDPHNLGACLRSADACGALAVIVPRDRAAQLTATVRKVAAGAAETTPVVAVTNLV
                     RTLKLLKDAGLWIVGADAAAAVPAREVDLKGPVVLVLGAEGTGLRHSTRQNCDFLVSL
                     PQRGAVESLNVSVAAAMLLYEALRQRDLS"
BASE COUNT          952 a         1634 c         1696 g          915 t
ORIGIN      
        1 catgtccatg gttcgcaaac ttcgcgcgcg cttcgacgtc tcagcggatc tcttgattgc
       61 accaaggcga tcgcgcggcc ttgccgccta gtgccacttg cgtcggtggc agccacgccc
      121 gcgcctcata caacagactg atccccccta cgagatcacg gacgtgactg agaaaccgtc
      181 gctcacgacc gccggccgac tgtcccacaa ggtcaagcca gctgatcgcg gcaagctcaa
      241 gatcggcgac gactggaacg cgatccgtat catcgcgctt tcgcagagca accccttaaa
      301 ggcaatagcc gagttcgtcg agaacagcat cgatgcgcat tcgaagacga ttacaatcac
      361 gcggggccgc gagcacaggg aacactacct cttgatcaag gacgatggtg acggtgtgcc
      421 gcgcgacgaa ggtggccggc cagatttcaa atacgtcgcc acgcacatct gcgattcgat
      481 caagcggcgg ttgaaagcgg aaggcgaagg caagagcgta caaggggagt ttggcatcgg
      541 tctgctgtct ttctggacgg tgggcaattc gctcacgatg atttcgacgg gggccgacca
      601 gcgctcgtac gagatgacca tgagcaaggg cgactcgggt tacgccgtgc ggccgcgccg
      661 cgttctcatc gccgagcgcg gcaccgagct gcgcatcagc ccattgctcg acggaatccg
      721 caacctgtct ggagagaaga tccagtggta tctggcatcc gaactccgtg accgaattcg
      781 taaggccaaa gtccgcgtta ccgtcattga cagactggcg cgcaagcaat atgaagtcga
      841 gcctcgcgtc tacgaaggac ggctgttgca tcagctacct gccgtgacga caacgtatgg
      901 tgaagcatac gccgagctgt atctcaccga gccggacgag aactgtcgcg tggcactgtc
      961 gcgggctggg acccgtgtga tcgaggatct caccacacta cccggtctcg accattcccc
     1021 gtggacctcc cgatacttgc agggactcat cgacgcgccc tttctgaacc tgacgccgag
     1081 cacccgcagc ggcatccttc acgatgagcg ttacgctgcg ctcgcacaag cgctgccgcc
     1141 gctggagcag cagctgaatg cgctgattga ggcgcagcaa cgggccgaag aggaggaggc
     1201 gagccgcgaa tccctgcgcg ctatccagcg agcgttccgc gaagcgctct tggcgcttcc
     1261 gcccgaggag tacgactggt tcgaaattca ggcgcgagct cgaagcgata gcggacctga
     1321 aacctcaggg gttgggaacg gaacatttac ggccgcggag gcgcagtccg ctgaacttgg
     1381 agtagcggag ccggaactgc atccaggcga acagcgtcag ttctttgaat acgcaggccc
     1441 actcttcagt gtcgttatct cgcccgcttc cagcacgaca ccgttgaacg aacgtaaaaa
     1501 gttccgcgcc ttgccgcgcg atcgctcgcg ccgccgcgtg gtggaggatc tccagttctc
     1561 atgggagatt gtcgaaggcg gcggatcgct ttctgacacg cggaatcagg aaatcgagta
     1621 tctagcgccg gccaacccgg gacttgcacg actcaaggtg tcggtgacgc agcgcgagat
     1681 cacttgcgtc gcagaggctc tcatcacaat aactgcgacg ctggagacct ccgtaggctc
     1741 aacggttgta actgcgcggg gacttcccgg ctataccttt gagcgcgcgg cgggggagtt
     1801 atggcgctcg cgcttcgatg cagctcgcaa cctcataatc gtcaacaacg ggcatcgtga
     1861 ctttgtattc gcccgtagga accgagcact tcagctgcgc tatcttgtcc gcctctacgt
     1921 gaaggagcta gtgctgagaa acttcgctgg cgctgcctcc gagcagctcc ttgagcgcat
     1981 gatcgagctg acactctatg cggaagagaa gttgaaatcc ggctagcgcc atggctggct
     2041 tgtgaggcca gaaccacatc tataaatggt tataattact tgataactag agaaattctg
     2101 gtgcccggga gaggactcga acctccacgg agttacccgc tagtacctga aactagtgcg
     2161 tctaccaatt ccgccacccg ggcaggtgag gaaggccgcg caatgtacgc acgggttccg
     2221 gggctgtcaa ttgaagggac gtaaggcaca gttcggacgt cgcggccgca gcggcgctgc
     2281 agcggcaaag ccccgaagag gcgagacgca gcgcgagaaa ccagcgtcgt cttccccggc
     2341 gcgcacgcgc gcgagcaccg gtgtggcgag ctcgcgagca cgacacactg cttcctccct
     2401 gcggccgggg acgattgtcg aaggcacagt gagtgccaac cgtgccggct acggctttct
     2461 gcgcgtggaa ggcgcgggcg agagcgtgtt cctgccgccg ccgcaaatgc gcggtgtgat
     2521 gcacggcgac cgcttgcgag tcagggtgtc gcgcgatggc agcgcacgtt ggtcgggcac
     2581 cgtcgagcag gtcatctcgc gcggagtcaa ctcctttctt ggaacgctcg agatccaggg
     2641 acgcagcgcc tgggtgaatg ccgccgatcg gcggctgcag ctgcgctgcg cggtggcgcc
     2701 gccggacctg cacggtgcgc gtcacggtga ctgggcgatt gcacgcatca cgcgccacgc
     2761 cggctccgct tccgccgccc aggcgcgcat cgagaaggtg ctcgatcccg accgtccagt
     2821 ggagctcgcc accgaatccg ccatcgcccg cttcgatctg ccgcacgagt ttccagccga
     2881 gacgctgcgc gaggcgcggg ttttcggcga gcatgtcgat ccacgcgagg ccgcagcacg
     2941 cgtcgatctg cgtgagctgc cgctcgtcac catcgatggt gaggacgcgc gtgacttcga
     3001 tgatgcggtt tatgcggagc cgcatgcagg gggttttcgt ctcatcgtgg cgattgccga
     3061 tgtcagccac tacgtgcgcc cgggaagtgc tctggatgcg caagcgcagc agcgcggcac
     3121 ctcggtgtac ttcccgacgc gggtgctgcc gatgttgccg acggcactct cggatcatct
     3181 gtgctcgctc gcgccgcacg tcgagcggtt gtgctttgcg gccgacatga tcgtgagccg
     3241 gagtggggcg ctcaagagcg cgcgtttcta tccggccgtg atgcgctcgg cggctcgtct
     3301 cacctacacg ctcgcgaacc aggcgctgtt cgaggggcgc ccggcagcgc gcgtgaagtt
     3361 gggcgcgctg gtcgaacgcc tcatggtgct ggtggatgtt taccgtgctc tgtaccgggc
     3421 gcgggcgcgt cgcggtgcgc tcgatttcga tgcagcggaa gccgaattcg tgatcgatgc
     3481 cggtgagcac gtgcgcgcga ttgagctgcg cgtgcgcaac gacgctcacc gcctgatcga
     3541 ggagtgcatg atcctcgcca acgtcgcggt agcgcgcgag ttggagactt cgcacacacc
     3601 cacgctgtat cgcgtgcacg gcgtgcctga ggatgagaag ctcgagcgcc tcgcatcgac
     3661 tctcgcttcc ctcggcatcg acgcgcgtat ccccaagacc gtgaccactc gcgacctgca
     3721 ggcgatagcg cgccgcgtga gcgatgcctc cgagcgcacc ttcatcgaat cgctggtcgt
     3781 ccgcgccatg ccccaggcca tttatcaacc caccaacatc gggcacttcg ggctggcgct
     3841 cactcattat gcccacttta cttccccgat ccgccgctat cccgacctga tcgtgcatcg
     3901 caccctcaag gcacgcatca aagcttccgg cggcgcgggc cggcgctacg cggtgggcga
     3961 gctcgggcca ctcggtgaga gcacctcgcg gctcgagaag cgcgccgatg aagccgatcg
     4021 ctacgtatca gggttcctga aatgcaccta tttaaaggag cgcatcggcc agacgttcca
     4081 gggtctcatc accacggtcg tggagttcgg ctgcttcgtg cagatcctgg acgtcgcggt
     4141 agacgggctg ctgcacatca acaacttgca cgatgacctc tacgtgatgg aagaggacgg
     4201 tcacgcgtgg cgcggccgtc gcagcgggcg gcggctgcgc accggcgcgc acgtacgcgt
     4261 catcgtcacc gcggtgaatc cgatcgaggg cctcatcgac ctggcgctcg ccgaggacgg
     4321 ctgaggttgc cctcgctcgg cgatgcggca tcgccatgaa acaaagcgaa accatctacg
     4381 gaatgcacgc ggtgcgggta atgctcgagc gccacgcgca acgcgtgctc aatgtgcgcc
     4441 tggcggcgca gcgcgatgac ccgcgcgccc gggagatcga gcagttggca cgcgaggccg
     4501 agcggccggt gcagcgcatc gatatgcatg cgctccgtca gcagctcggc gctgtggcgc
     4561 accagggggt tgccgccgag atcactccgc ttcccccatg gagcgaagat gagctgctcg
     4621 aggcactaca gtcggcgcgc gcgcccttgc tgcttgcgct cgatggtgtt caggatccgc
     4681 acaatctcgg cgcctgcctg cgcagcgccg atgcctgcgg cgcgctcgcg gtgatcgtgc
     4741 cacgcgaccg cgcggcgcaa ttgacagcga ccgtgcgcaa ggtggccgcg ggggccgcgg
     4801 agacgacgcc ggtggtcgcg gttacgaatc tcgtgcggac gctgaagctg ctgaaggacg
     4861 cggggctatg gattgtcggt gccgacgctg cggctgcagt gccggcgcgc gaagtggacc
     4921 tgaagggacc ggtggtactc gtgctcggcg cggaaggcac ggggctgcga cactcgacgc
     4981 ggcagaactg cgattttctc gtgagcttgc cgcagcgggg cgcggtcgag agcctgaatg
     5041 tctcggttgc cgcggcgatg ttgctctacg aggcgctgcg gcagagggac ctctcatgaa
     5101 ggccgtgctg gcgaggactt tcggaccccc cgaagagctg cgactcgagg aggttccgcc
     5161 gcgacacacg ggcgccggcg aagtcctcat ccgggtg
//
LOCUS       DATDMP010000325         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_495703, whole genome shotgun sequence.
ACCESSION   DATDMP010000325 DATDMP010000000
VERSION     DATDMP010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495703"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..837
                     /locus_tag="VI195_05655"
     CDS             127..837
                     /locus_tag="VI195_05655"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6823904.1"
                     /translation="MNSRRTSPAAGKAAASPWRTSRERLRDRELKRDAVIRAAAREFN
                     RKGYHNTSLDDIAAALEVTKPTVYYYVTSKEQLLFECFVAGVEQIRAAFREVRQLPVP
                     ARERLNAVLRHYGEAVASEFGWCMVRAEDQDLAPAMSRHIKSLKSEIDQGIRRLVREG
                     IQDGSIEPCDPKMTAFALAGALNWIAHWYREDQSLTGAQIAAAFVTVFEGGLRPRRSA
                     LRIDARARRRRTQGTLAR"
     gene            861..2045
                     /locus_tag="VI195_05660"
     CDS             861..2045
                     /locus_tag="VI195_05660"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY6823905.1"
                     /translation="MSPAEVVIVSAKRTPIGAFLGALSPLSAVQLGSAAIKAALEAAT
                     LGAADAQEIIMGCVLPANLGQAPARQAALGAGVPIATPATTINKMCGSGLKAVMMAAD
                     QIRAGSAGIVVAGGLESMSNAPYLLPKARTGYRMGHGEIIDHMMKDGLQSPFDGKAMG
                     CFADATASRYNFSRAQQDAFATESVRRAMQALQAGEFDVEVTPVTVKGRKGETVIARD
                     ETPGTCEVSKITSMRPAFSKDGTVTAASSSSISDGAAALIVMSAQTAAERGLKPLARI
                     LSYASHAQEPEWFTTAPVGAIRKVLTQLNWKPHDADLYEINEAFAVVTMAAMRDIDID
                     HARVNVNGGACALGHPIGATGARILTTLIHTLRRRGGRRGIASLCIGGGEAVALAVET
                     IN"
     gene            2077..>2397
                     /locus_tag="VI195_05665"
     CDS             2077..>2397
                     /locus_tag="VI195_05665"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6823906.1"
                     /translation="MKLSDTRAVITGGASGLGLAVARHLSAAGGKVALLDVQEAPGRA
                     AAAGLGSGAAFVRCDVTSEPEVNDSMETAQARLGGLNLLVNCAGVVGAGRLLGKNGPM
                     SGEFF"
BASE COUNT          392 a          834 c          805 g          366 t
ORIGIN      
        1 ctttactcac gggtaggaat gctatactga cgagtatgat gccgcgctcg ggcccggtca
       61 acgcactgcc cggagcgtat cgccacctga cgcacccggc cgcgccgcta taatactctc
      121 accgccatga acagccgccg taccagtccc gcagcgggca aggccgccgc ctcgccgtgg
      181 cgcacgagcc gcgagcggct gcgcgaccgc gagctcaagc gcgatgccgt catccgcgcc
      241 gcggcgcgcg agttcaaccg caagggctac cacaacacct cgctcgatga catcgccgcc
      301 gccctcgagg tcaccaagcc gacggtgtac tactacgtca ccagcaagga gcagctgctg
      361 ttcgagtgtt tcgtggccgg cgtcgagcag atccgcgctg cgttccgcga ggtgcggcag
      421 ctgccggtgc cggcgcggga gcggctgaat gcggtgctgc gtcattacgg cgaggcggtc
      481 gcctcggagt tcggctggtg catggtgcgc gcggaggacc aggatctcgc gcctgccatg
      541 agccgccaca tcaagtccct caagtccgag atcgaccagg gcatccgccg gctggtgcgc
      601 gagggcatcc aggacggctc catcgagccc tgtgacccga agatgaccgc cttcgcgctc
      661 gccggcgccc tcaactggat cgcgcactgg tatcgtgagg accagtcgct caccggcgcg
      721 cagatcgccg ccgcgtttgt caccgttttc gagggggggc tgcgtccgcg ccgcagcgcc
      781 ctccgcatcg atgcccgcgc ccgccgccgg cggacgcagg gcacactggc gcgctgacac
      841 gaagtcaaag cgaggctgcc atgtcccctg cagaagtcgt catcgtatcc gccaagcgta
      901 cgcccatcgg cgctttcctc ggcgccctct cccccctcag cgccgtccag ctcggcagcg
      961 ccgccatcaa ggcagccctg gaagctgcca cgctgggcgc cgcggacgcg caggagatca
     1021 tcatggggtg cgtgctgccg gcgaacctcg gccaggcgcc ggcgcggcag gcggcgctcg
     1081 gcgcgggcgt gccgatcgcc acccccgcca ccaccatcaa caagatgtgt ggctcgggcc
     1141 tgaaggcggt gatgatggcg gccgatcaga ttcgcgccgg cagcgctggc atcgtggttg
     1201 ccggcggtct ggagtcgatg agcaacgccc cctatctgtt gcccaaggcc cgtaccggct
     1261 accgcatggg gcacggcgag atcatcgatc acatgatgaa ggacggtctg cagagcccct
     1321 tcgatggcaa ggcgatgggc tgcttcgcgg acgccaccgc gagccgttac aacttcagcc
     1381 gtgcgcagca ggacgccttt gcgaccgagt cggtgcgccg cgccatgcag gcgctgcagg
     1441 caggcgaatt cgacgtggag gtgacgcccg tgacggtcaa gggccgcaag ggtgagaccg
     1501 tcatcgcacg cgatgagacg cccggcacct gtgaggtgag caagatcacc tccatgagac
     1561 ccgccttcag caaggatggc accgtgactg cggcgagctc ctcctcgatt tccgatggag
     1621 ccgcggcgct gatcgtgatg agcgcgcaga ccgccgccga gcgcggcctc aagccgctcg
     1681 cgcgcatcct gtcctacgca agccatgcgc aggagccgga gtggttcacg acagccccag
     1741 tcggcgccat ccgcaaggtt ctcacgcagc tcaactggaa accgcacgat gcggacctct
     1801 acgagatcaa cgaggctttc gccgtggtca ccatggcggc aatgcgcgat atcgacatcg
     1861 atcatgcgcg cgtcaacgtg aacggtggcg cctgcgctct cggtcacccg attggcgcga
     1921 ccggcgcgcg cattctcacg accctcatcc acacattgcg ccggcgcggc gggcggcgcg
     1981 gcatcgcctc gctgtgcatc ggcggcgggg aagcggtggc gctggccgtg gagacaatca
     2041 actgagggat cgcacactgc gcgtacgcgg acctccatga aactaagcga cacacgcgcg
     2101 gtaatcaccg gcggtgcctc gggcctcggt cttgcggtcg cgcgccacct cagtgcagcg
     2161 ggcggtaagg tggcgctgct cgatgtgcag gaggcccccg gccgcgccgc cgcggccggc
     2221 ctcggatcag gtgccgcctt tgtgcgctgc gatgtgacct ccgagcccga ggtcaacgac
     2281 tccatggaga ccgcgcaggc ccgcctcggg ggtttgaatc tcctcgtgaa ctgtgccggc
     2341 gtggtcgggg ccggacggtt gctcggcaag aatggcccca tgtcggggga gttcttt
//
LOCUS       DATDMP010000326         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1718627, whole genome shotgun sequence.
ACCESSION   DATDMP010000326 DATDMP010000000
VERSION     DATDMP010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718627"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VI195_05670"
     CDS             <1..652
                     /locus_tag="VI195_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6823907.1"
                     /translation="EVAAGESLAIVGRSGSGKSTLVNLLPRFYDVRSGSVAVDGRDVR
                     EYELRSLREQIAVVSQDVVLFDDTIRNNIAFGRQVSDAAVERAAEAAHILEFVRNQPG
                     GLDALVGDRGMLLSGGQRQRIAIARALLKDAPILILDEATSALDTESERHIQAALAQL
                     LRNRTTFVIAHRLSTVEQADRILVLNAGSVAENGTHAQLLAHGGLYAQLHRLQFSD"
     gene            667..1695
                     /gene="lpxK"
                     /locus_tag="VI195_05675"
     CDS             667..1695
                     /gene="lpxK"
                     /locus_tag="VI195_05675"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY6823908.1"
                     /translation="MERQLTQLWYREHPRASLLQPLAWLYRGITSLRARLYRSGWARS
                     MSAGRPVVVVGNLTVGGTGKTPLTLWLAGALSAQGLRVGIVARGYGGRSGSGPRRVQA
                     DSIWQDVGDEPLILARRSGCPTVVGGDRLAAARALVALGVDVILADDGLQHLRLARDC
                     EIVLIDGARGFGNGRLLPAGPLREPTARLLRVDAIVVNGDATHRSLAALPAGRALTMQ
                     LQVGEAVSVDERRRQPLAAFRGARVHAVAGIGNPQRFFAQLRAHGLEVIEHAFPDHHP
                     LSAAELAFADSLAVLMTEKDAVKCAPFADQRLWYVPVTAQLAAPDAQQLLSHILPKVR
                     GAAGAGGP"
     gene            1698..1886
                     /locus_tag="VI195_05680"
     CDS             1698..1886
                     /locus_tag="VI195_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY6823909.1"
                     /translation="MDAHLLEIIACPVCKGPLQFRRDAQVLVCRMDRLAYPIRDAVPV
                     MLEEEARQLAADDPLLDR"
     gene            1929..>2177
                     /locus_tag="VI195_05685"
     CDS             1929..>2177
                     /locus_tag="VI195_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY6823910.1"
                     /translation="MVIPARFASTRLPGKALLPIAGKPMLQWVYERARAAGAQEVLIA
                     TDDERIASAARGFGAQPVMTASTHASGTDRIAEVARARR"
BASE COUNT          324 a          740 c          767 g          346 t
ORIGIN      
        1 cgaggtcgcc gccggcgaga gcctggcgat cgtcgggcgc tcgggcagcg gcaagtcgac
       61 gctggtgaat ctgctaccgc gcttctacga cgtgcgtagc ggcagcgtcg ccgtcgacgg
      121 ccgcgacgtg cgcgagtacg agctgcgaag cttgcgcgag caaatcgcgg tggtcagcca
      181 ggacgtggtg ctgttcgatg acaccatccg caacaacatc gccttcgggc gccaggtgtc
      241 ggacgcagcc gtggagcgcg ccgccgaggc ggcccacatc ctcgagttcg tgcgcaacca
      301 gccgggcggg ctcgatgcgc tggtgggcga tcgcggcatg ctgctctcgg gcggacagcg
      361 ccagcgcatc gccatcgcac gcgcactgct gaaggacgca ccgatcctca tactcgatga
      421 agcgacttcg gccctcgaca ccgagtccga gcggcacatc caggccgccc ttgcccagtt
      481 gctgcgcaat cgcaccacct tcgtcatcgc gcaccgcctg tccacggtgg agcaggccga
      541 ccgcatcctg gtgctcaacg ccggcagcgt cgccgagaac ggcacgcacg cgcagctgct
      601 ggcgcacggt ggcctgtacg cgcagttgca tcggctgcag ttctccgact gaggagctgg
      661 gcgacgttgg agcgacagct gacgcagctg tggtatcgcg agcacccgcg ggcgtcgctg
      721 ttgcaaccgc tcgcctggct gtaccggggg atcacctcgc tgcgcgctcg cctgtatcgc
      781 tcgggctggg cacgcagcat gagcgccggt cgtccggtcg tggtcgtggg caatctcacc
      841 gtcggcggca ccggcaagac accactcact ctctggctgg ccggcgcgct cagcgcacag
      901 ggactgcgcg tcggcatcgt cgcgcgcggc tatggcggcc gcagcggcag tggaccccgg
      961 cgcgtgcagg cagactcaat ctggcaggat gtgggagatg agccattgat tctggcgcgc
     1021 cgcagcggtt gcccgaccgt ggtcggcggc gatcggctcg ccgcggcgcg cgccctggtt
     1081 gctctcggtg tggacgtcat cctcgccgat gacggactgc agcatctgcg gctcgcgcgc
     1141 gactgcgaga tcgtgctgat cgatggcgcg cgcggtttcg gcaatggccg cctgctgccg
     1201 gccgggccgc tgcgcgagcc aaccgcgcgg ctgctgcgcg tggatgcgat cgtcgtgaac
     1261 ggcgacgcaa cccaccgctc actggctgcg ctgcctgcgg ggcgcgcgct cacgatgcaa
     1321 ctgcaggtgg gcgaggcggt cagcgtggat gagcgccgtc ggcaaccgct cgcagcattt
     1381 agaggtgcgc gcgtacacgc cgtggccggc atcggcaatc cgcagcgctt cttcgcgcag
     1441 ttgcgggcgc acggactgga agtcatcgag cacgcgtttc ccgatcatca tcccctcagc
     1501 gcggcagagt tggccttcgc cgactcattg gccgtgctca tgaccgagaa agatgccgta
     1561 aaatgcgcac cgttcgccga tcagcgactg tggtacgtac cggtcactgc gcagctcgcc
     1621 gcgccggacg cgcagcagct gctcagccac atactgccga aggtgcgcgg cgccgcgggc
     1681 gctggaggtc cttagctgtg gatgcacact tgctggaaat catcgcctgc ccggtttgca
     1741 agggaccgct gcagttccgg cgcgacgcgc aggtgttggt ttgccgcatg gaccggctgg
     1801 cctatccgat ccgcgacgcc gtaccggtca tgctcgagga agaggcacgg cagctggcgg
     1861 ccgatgatcc gctgctcgat cgctgagcag tgccgccgca gcgtccgcaa gttccgcaat
     1921 cattccgcgt ggtgataccg gcgcggtttg cctccacgcg gctgcccggc aaggcattgc
     1981 tgcccatcgc cggcaagccg atgctgcagt gggtgtacga gcgcgcccgc gccgccggtg
     2041 cacaggaggt gctgatcgcg accgacgatg agcgcatcgc cagtgcggcg cgcggcttcg
     2101 gggcgcagcc ggtgatgacg gcgagtaccc acgcctccgg caccgaccgg atcgcggaag
     2161 tggcgcgtgc acggcgc
//
LOCUS       DATDMP010000327         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1829777, whole genome shotgun sequence.
ACCESSION   DATDMP010000327 DATDMP010000000
VERSION     DATDMP010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829777"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VI195_05690"
     CDS             complement(<1..656)
                     /locus_tag="VI195_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY6823911.1"
                     /translation="MNRVSWFGMTTAVCLSLAASLTQAATPTATAQRAHLRYSNRPAL
                     NDPALRSSAALVLDLSNSAVLYSRRSEVALPIASITKLMTALVVMDADQSLDEMLSVT
                     EDDRSSGRGAFSRLAPGTQLTRGDLMHLALMASENRAAHALGRTYPGGIAACVTAMNA
                     KARELGMTNAHFVEPTGLSDENVASPEDLSKLVLAAAKVAAIREYSTDSDYTVHVGRH
                     L"
     gene            complement(847..1410)
                     /locus_tag="VI195_05695"
     CDS             complement(847..1410)
                     /locus_tag="VI195_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823912.1"
                     /translation="MITIASLWLPILVSAVIVFVASSVIHMGPLWHRSDYPQMPREAE
                     VLDALRPLAIPPGDYFIPRAGGMQEMRSPEFKHKLNQGPVAVMTVMPNGMFSMQRNLL
                     QWFVFLIVVSVFCAYIAGRTLPAGTPYLRVFQIVGATAFIAYALALCELSIWYRRAWS
                     LTLKGALDGLIYGALTAGTFGWLWPHS"
     gene            1747..>2568
                     /gene="rpoH"
                     /locus_tag="VI195_05700"
     CDS             1747..>2568
                     /gene="rpoH"
                     /locus_tag="VI195_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY6823913.1"
                     /translation="MTAGALVARPGDLALAGPLGSLDAYLERVGRIPVLSREDERALA
                     ERFRSQEDLQAARELVLSHLRFVVHIARGYSGYGLPMGDLIQEGNVGLMKAVKRFDPR
                     LNVRLVSFAVHWIRAEIHEYVLRNWRLVKIATTKAQRKLFFNLRRLKKNLSWLSAEET
                     LAVARDLGVSVGEVTEMEKRLAARDLSFDPAPEADDDELYSPAAYLPAPEADPAEQVE
                     EAESADDSSQRLRGALGRLDARSRDIVQRRWMSEDKATLHELADKYGVSAERIRQI"
BASE COUNT          454 a          826 c          845 g          443 t
ORIGIN      
        1 aggtggcggc cgacgtgcac ggtgtaatcg ctatcggtgg agtattcgcg aatggcggcc
       61 actttcgccg cggccagcac cagcttggac aggtcctccg ggctcgccac gttctcatcg
      121 gagagcccgg tcggctcgac gaagtgcgcg tttgtcatgc cgagctcgcg cgccttggcg
      181 ttcattgcgg tcacgcaggc cgcgatccca cccggatacg tgcgcccgag tgcatgcgcg
      241 gcgcgattct cggaggccat cagcgcgagg tgcatgaggt ccccgcgcgt gagctgcgtg
      301 ccgggcgcca ggcgcgagaa cgcgcccctg ccggaggagc ggtcgtcctc ggtgacgctc
      361 agcatctcat cgagcgactg gtcggcgtcc atcaccacca gggccgtcat gagcttggtg
      421 atcgaggcga tcggcagcgc gacttccgag cggcgtgaat agagcaccgc agagttgctg
      481 agatcgagca ccagtgcggc gctcgagcgc agtgccgggt cgttgagcgc ggggcggttg
      541 ctgtacctga gatgcgcccg ttgcgcagtg gcagtaggag tagctgcctg cgtgagtgag
      601 gccgcgagcg acaggcatac cgctgtcgtc ataccgaacc agctgactcg attcatcgac
      661 ccctgcgcct cactccccga gttagttgag tgtatcagag catgctttat gtcatgcatt
      721 cgtgacgcac tcttgagttt tcacgcgctt tatatcacag cccttgcagc tcgttcgggc
      781 gtgcggtcat aacacggacg gcgcgtgacg cagcgctcgc cggcacctgc cggcagcgtg
      841 cgtccatcac gagtgcggcc agagccagcc gaaggtgccc gcggtgagcg ctccataaat
      901 aagtccgtcg agcgcgccct tcagcgtcag gctccaggcg cgccgatacc atatggagag
      961 ctcgcacagc gcgagcgcgt aggcgatgaa cgcagtggcg ccgacgatct gaaatacccg
     1021 caggtacggc gtgcccgcgg gcagcgtgcg tccggcgatg taagcgcaga agacgctcac
     1081 cacgatgagg aacacgaacc actgcagcag gttgcgctgc atcgagaaca tgccgttggg
     1141 catcaccgtc atcaccgcga ccggaccctg attcaatttg tgcttgaact ccggcgagcg
     1201 catctcctgc atgccaccgg cgcgtggaat gaagtaatcc cctgggggga tggcgagcgg
     1261 ccgcagcgca tcgagcacct cggcctcccg cggcatctgc gggtagtcgc tccgatgcca
     1321 gagcggtccc atgtgtatga ccgagctcgc gacgaacacg atcaccgccg acaccaggat
     1381 cggcagccac agcgatgcaa tggtgatcat cgggcctccc ccctcggtta tgtccgggcc
     1441 ccgtggaacc cagcggcttt cgcccgactc taccgagaaa cccttcgggc gttaattcgg
     1501 ctgcggccga gtcgaagcgg aggcgccacg gcgcccatgg ccgcgtccag gctcaggatt
     1561 gatctgcgcg tccggtctct tccgaggtca ccggaccacc ccggaagggg gattaccccg
     1621 ggggctgccg gcatacaagc tattgttcag caagggtgtt gagccacccg gcttgggaac
     1681 ttacggagcg tttagcactc taatgaggcg actgctaaaa tgcccatccg aaccacggga
     1741 gatctaatga ctgcaggtgc actggttgcc cgtccgggcg atctggcctt ggccggtccg
     1801 cttggaagtc tcgacgccta cctcgagcgc gtgggccgta ttccggtcct cagccgggag
     1861 gatgaacgcg cgctcgccga gcgctttcgc tcgcaggaag acctgcaggc cgctcgggag
     1921 ctcgtgcttt ctcacctgcg cttcgtggtg cacatcgcac gaggctacag cggctatggc
     1981 ctccccatgg gagacctgat ccaggaaggc aacgtcgggc tgatgaaggc ggtgaaacgc
     2041 ttcgatccgc gcctcaacgt gcggctggtt tccttcgcgg tgcactggat ccgcgccgag
     2101 atccacgagt acgtactgcg caactggcgt ctggtgaaga tcgccacgac caaggcgcag
     2161 cgcaagctgt tcttcaacct gcgccgtctg aagaagaacc tcagctggct gtccgccgag
     2221 gagacgctcg ccgtcgcgcg cgatctcggc gtatccgtgg gcgaggtcac ggaaatggag
     2281 aagcgcctgg cggcgcgtga tctctccttc gatccggccc ccgaagcgga cgacgatgag
     2341 ctctacagtc cggctgccta tctgccggca cccgaggcag accccgccga gcaggtggag
     2401 gaggccgaga gcgccgatga ttcctcgcag cggctgcgcg gcgcgctggg ccgcctcgac
     2461 gcacgcagcc gcgatatcgt gcagcgccgt tggatgtcgg aagacaaagc gaccctgcac
     2521 gagctcgccg acaagtacgg cgtgtcggcg gagcgcattc gtcagatc
//
LOCUS       DATDMP010000328         4017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_830084, whole genome shotgun sequence.
ACCESSION   DATDMP010000328 DATDMP010000000
VERSION     DATDMP010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830084"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VI195_05705"
     CDS             <1..363
                     /locus_tag="VI195_05705"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEY6823914.1"
                     /translation="EDITGGVQVCSSFNGATCTASPWEQGWIVLPSVNPFNPAAAPKP
                     LQVVGALPAGTTLTELNGNLSVTFLSNGVANTAVAFKMCDSRGASYARYLQVTLMGRV
                     VAAPIAGQDLTGAPLACP"
     gene            377..898
                     /gene="pilV"
                     /locus_tag="VI195_05710"
     CDS             377..898
                     /gene="pilV"
                     /locus_tag="VI195_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY6823915.1"
                     /translation="MSPTDIRSAGGRAEKGFTLLEVLIAMLVLSIGLLGIGKLMMLSA
                     RANDSAYMRTQATTLAYTILDAMRANRQAALAQGYDTAMGAFPGPVACTAAAPCNSGQ
                     QAQNDLNLWGARLAAALPQGQGSVATATAAGATGANNVTATVTVQWSDKVAEQSFGAP
                     TGNLLSITLETLL"
     gene            895..1965
                     /locus_tag="VI195_05715"
     CDS             895..1965
                     /locus_tag="VI195_05715"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY6823916.1"
                     /translation="MKRQAGFSMVELMVAIVLAMILTGAVIAVFVGSRTAYQATAGVG
                     EMSDSGRFALNLIGESVRSAGNLACNSAMSATSQAVLPVGTTFTNNYGQGIAGFEANG
                     TTPPAALSLPAVPAAGAANNWTPNLDPAVTGAAAAGPFGFPVKGSDVLVVRSSVPRVT
                     PVYTTADAAPGDSNVFVTPIPLAMAPLQYAAIADCTKSVYFQIAAVGAGLPANVSLNA
                     GVLPVGFSAGAVVAPLSTTVYYIGTGSDGDSSLWRIEQVNGPNFSAEELVPDIENMQV
                     LYGIDPSGSQTAAAYVTADQVGALNVVSIEVALLVASPPATKPVVAPLPYNVLGTQVT
                     APADNRVRRVFNATINLRNAVN"
     gene            1989..2561
                     /locus_tag="VI195_05720"
     CDS             1989..2561
                     /locus_tag="VI195_05720"
                     /inference="COORDINATES: protein motif:HMM:NF025061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEY6823917.1"
                     /translation="MRNTSVRFARSRQRGAVLYTALILLIVLTIIGVAAARLQTGEAI
                     MARNDHNHQLAMQAAEAALRDAEINLANGNWNVAQFAQNGAGLYVLQTEVQNPVNPGS
                     IVDGFNWNQNYPAPNPRAMTYTGPVLGNAPASPVLTQVIIENLPPVARAGDPLCTPSS
                     QTQSCSVYRVTAHAVGGDASASATLQSIIH"
     gene            2595..3041
                     /locus_tag="VI195_05725"
     CDS             2595..3041
                     /locus_tag="VI195_05725"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY6823918.1"
                     /translation="MGAQRHREAGVTLMELMVVVAIVGILAAIAYPAYTQFVRQTNRT
                     DATKTMQLFAQSLERCYSVNFKYAPCTVQGTVVNDGSTMRTPNLYYNVTFAIPDPQDY
                     TLTAVAIAAPQTADTQCAQFTLASTGQVAAQDAGANNTTQTCWGSN"
     gene            3074..3571
                     /locus_tag="VI195_05730"
     CDS             3074..3571
                     /locus_tag="VI195_05730"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY6823919.1"
                     /translation="MTGSNTWQQRDGGFTLIELILVVAIVALLASIAFPAYTSFTRQA
                     NRTDASNTMQLAARSLERCYARRLSYANCNVDGTIMNNGSTMQTPNLFYTITFTIPDA
                     RDYTLTAVPIQAPQTTDTQCAQFTISSSGAQAAPNNHGSDNTTTCWGANGTAGAGRSA
                     GKPGD"
     gene            3637..>4017
                     /locus_tag="VI195_05735"
     CDS             3637..>4017
                     /locus_tag="VI195_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY6823920.1"
                     /translation="MAAAQEPRLTAAIPVIEATIHDSGAEVAVAFRTLDGRAQWLRQA
                     DATFHAASTMKVAVLIELYRQARQGRVRLDEPLTIRNEFRSLVDGSPFALDPQDDSET
                     DLYKAVGETRTLRQLNELMITVSSN"
BASE COUNT          730 a         1393 c         1257 g          637 t
ORIGIN      
        1 gaagacatca ccggcggggt ccaggtgtgc agcagcttca acggtgcgac ctgtaccgcc
       61 tccccatggg agcagggctg gatcgtgctg ccgagcgtca atccgttcaa tccggcggcg
      121 gcccccaaac cgctccaggt ggtcggcgcg ctgcctgccg gcaccacgct cacggagctc
      181 aacgggaatc tgtcggtgac ctttctttca aacggcgtgg cgaatacggc ggtcgcattc
      241 aagatgtgcg actcacgcgg cgccagctat gcccgatacc tgcaggtgac cctgatgggg
      301 cgagtcgttg cagccccgat cgcggggcag gacctgacgg gtgccccgct cgcctgcccg
      361 tgacgaggtt aatcgcatga gccccaccga catacgcagt gcaggcggac gcgctgaaaa
      421 aggtttcacg cttctcgagg tgctgatcgc gatgctggtg ttgagcatcg ggctgctcgg
      481 catcggcaag ctgatgatgc tctcggcgcg cgccaacgac agcgcctata tgcgcacgca
      541 ggcgactacg cttgcgtaca cgatcctcga cgccatgcgc gccaatcgac aggccgccct
      601 cgctcagggg tatgacaccg ccatgggagc tttcccgggt cccgtggcct gcaccgcggc
      661 ggctccgtgc aattcgggcc agcaggcgca gaacgatctg aacttgtggg gtgcgcggct
      721 cgcggcggcg ctcccccagg gacagggcag tgttgcgacc gccacggctg ctggggcgac
      781 cggcgccaac aacgttaccg ccacggtgac cgtgcaatgg tccgacaaag tggccgagca
      841 gtctttcggt gcgcccacgg gcaacctgct atcgatcacg ctggagacgc tgctgtgaaa
      901 cgccaggcag gcttttccat ggtcgagctg atggtggcca tcgtgctcgc gatgattctc
      961 accggtgccg tcattgcggt gttcgtcggc tcgcgtaccg cctatcaggc gaccgccgga
     1021 gtcggcgaga tgtccgacag cggacgcttc gcgctcaacc tgatcgggga gtccgtgcgc
     1081 agcgccggaa acctcgcgtg caactcggca atgtccgcga ccagtcaggc ggtgctcccc
     1141 gtcggcacca cgttcaccaa caattacggt cagggcatcg cgggcttcga ggccaacggc
     1201 accaccccac ccgcggcgct ctccctgcct gcggtgccgg ccgccggggc tgcgaacaac
     1261 tggacgccga atctcgaccc ggccgtcacc ggggctgcgg ctgccgggcc ctttgggttc
     1321 ccggtgaagg gcagcgatgt gctcgtggtg cgctcgagcg tcccgcgcgt cacgccggtg
     1381 tacaccactg cggacgcggc gccgggcgac agcaacgtgt tcgtcacccc gatacccctt
     1441 gccatggcgc cgttgcagta tgccgccatc gccgactgca cgaagtcagt gtacttccag
     1501 atcgccgcgg tgggcgcggg cttacccgcc aacgtgagcc tcaacgcggg ggtgctgccg
     1561 gtcggcttct ccgcgggcgc ggtcgtggct ccgctcagca ccaccgtcta ctacatcggc
     1621 acgggctcgg acggtgactc ctccctgtgg cggatcgagc aggtcaatgg accgaatttt
     1681 tccgccgaag agctggttcc cgatatcgaa aacatgcagg tgctttacgg catcgatccc
     1741 agcggatccc agactgcggc cgcctacgtg accgcagacc aggtcggcgc gctgaacgtc
     1801 gtcagcatcg aggtggcact gctggtagcg agcccgcccg cgaccaagcc ggtggtcgcg
     1861 ccgctaccct acaacgtgct cggaacgcag gtcaccgcac cggccgacaa ccgcgtgcgc
     1921 cgggtgttca acgccaccat caacctgcgc aacgcggtca actgaagcgc gcctcacggg
     1981 agcccacagt gcgcaacacc tcagtacgct tcgcacggtc gcgccagcgg ggcgcggtac
     2041 tgtacaccgc cctcatcctg ctgatcgtcc tcaccatcat cggtgtcgcc gccgcgcgcc
     2101 tgcagaccgg tgaggcgatc atggcgcgca acgaccacaa tcatcagctg gcaatgcagg
     2161 ccgcggaagc ggcgctgcgc gatgccgaga tcaacctggc caatggcaac tggaacgttg
     2221 cgcagttcgc gcagaacggc gctggcctgt acgtgctgca gaccgaggtg caaaaccccg
     2281 tgaaccccgg cagcatcgtc gacggcttca actggaacca gaactacccg gcgccgaacc
     2341 cgcgcgccat gacctatacg gggccggtac tcggcaatgc gccggcatcg cccgtgctga
     2401 cgcaggtgat catcgagaac ctgcctccgg tagcgcgcgc cggcgatccg ctgtgcacgc
     2461 cctcgagtca gacccagtca tgttcggtat atcgcgttac cgctcacgcc gtcggcggcg
     2521 atgcgtccgc gagcgcaacc ctgcaaagca tcattcatta gacgctcacg tcaccatgca
     2581 tcggagagac tgagatggga gcgcagcggc accgcgaggc cggggtgaca ctcatggagc
     2641 tcatggtggt ggtcgccatc gtgggcatac tggccgcgat tgcctatccg gcgtacacgc
     2701 agttcgtcag gcagaccaat cgcacggacg ccaccaagac catgcagctc ttcgcgcaat
     2761 cgctcgagcg ctgctattcc gtcaacttca agtatgcgcc ctgcacggtg cagggcaccg
     2821 tggtgaacga tggctcgacg atgcggacgc ccaatctgta ctacaacgtc actttcgcca
     2881 tacccgatcc ccaggactac accctcacgg ccgtcgccat cgccgctccg cagaccgctg
     2941 acacccagtg tgcgcagttc acgctcgcca gcaccggtca ggtggcggcg caggacgccg
     3001 gcgcgaataa caccacccag acctgctggg gctcgaactg accccggact accgccggca
     3061 acgacagccg cgcataacag gatccaacac gtggcaacag cgtgatggcg gcttcacgtt
     3121 gatcgagctg atactggtag tcgcgatcgt ggcgctgctg gcttcgatcg ccttcccggc
     3181 gtacacgagc ttcacccggc aggccaatcg caccgacgcc agcaacacga tgcagctcgc
     3241 cgcgcgctcg ctcgaacgct gctatgcgcg ccgccttagc tatgcgaact gcaacgtgga
     3301 cggcaccatc atgaacaacg gctcgaccat gcagacgccg aacctgttct acaccatcac
     3361 gttcacaata cccgacgccc gggactacac tctgaccgca gtaccgatcc aggcgccaca
     3421 gacgacagat acccagtgcg ctcagttcac gatctcgagc agcggcgcgc aagccgcgcc
     3481 gaacaatcac ggcagcgaca acaccacgac ctgttggggc gcgaacggca cggctggggc
     3541 gggacggtcc gccggcaaac caggagactg acccagcaac tcgcgcgcgg cgcacagcgg
     3601 tgcctgctgg cctgcggcgc gctgctctat gccggcgtgg cggctgcgca ggaaccacgg
     3661 ctcaccgcgg ccattccagt gatcgaagcc accatccatg acagcggggc ggaggtggcc
     3721 gtcgcttttc gtaccctcga cggccgtgcg caatggctgc gccaggctga tgccaccttc
     3781 cacgcggcga gcaccatgaa ggtggcggtg ctgatcgagc tgtaccgcca ggcccggcag
     3841 gggcgggtgc gcctcgatga gccgctcacc atccgcaacg agttccgcag tctcgtggat
     3901 ggctcgccgt ttgcgctcga tccacaggat gactcggaga ccgacctgta taaggccgtc
     3961 ggcgagaccc gcacgctgcg gcagctcaac gagctgatga tcaccgtgag cagcaac
//
LOCUS       DATDMP010000329         3950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1830222, whole genome shotgun sequence.
ACCESSION   DATDMP010000329 DATDMP010000000
VERSION     DATDMP010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830222"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VI195_05740"
     CDS             complement(<1..544)
                     /locus_tag="VI195_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6823921.1"
                     /translation="MRGSGASFDDIIERERGLRRSLTARRMSMIAIGGAIGTGLFLGS
                     GFAISLAGPAVLLSYLLGALIALLLMGCLAEMTVAHPTSGSFGAYAEYYLGPLAGFLV
                     RYAYWTSIVLAVGTEVTAIALYMHYWFPHSPGWPWIVGFSALLVLVNARSVKVFGAIE
                     YWFSAIKVAAILMFILLAAYI"
     gene            618..1841
                     /locus_tag="VI195_05745"
     CDS             618..1841
                     /locus_tag="VI195_05745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6823922.1"
                     /translation="MLGYFRLLTRPQLHAYLAALGGWALDALDFFIFVFCLKAISGEF
                     HTDVKAVSEGIFLTLACRPLGALAFGWLAEKYGRRPVLMLNVVSYSLVQLASAFAPNL
                     ATLLALRALFGFAMGGEWGVGAALALETLPARGRGFFSGLLQEGYVIGYLLASVVFAT
                     LFERVGWRGLFIVSSVSALLVLYIRFGVQESPAWVAGGAPQRASAVLLWGAARDYFPT
                     LAYLVLLMACFNAYSHGSQDLYPTFLQVQHGFSAATTGKIAIVMNLGALAGGICFGSL
                     SERLGRRRAIALATLLSVPMIPLWAYSHTAVMLALGAFLMQFMVQGAWGIVPAHLNEL
                     SPPSVRAVLPGFAYQLGNLAMAKMAPIQSGLAEARGGDYAYILASTMVVVAAALIIVT
                     LLGPERRSAELRVVS"
     gene            1858..2355
                     /locus_tag="VI195_05750"
     CDS             1858..2355
                     /locus_tag="VI195_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823923.1"
                     /translation="MRIPALVLAAALAAAAHCRSAPAQAPGPYEALAALAGEWEATLQ
                     GFGKLTNSIRLVSKGTAIEETIGTPADNETSVYTRDGASVLLTHFCALTPAGHIARLA
                     LRARQGTQPLEFVFVGASNLPELDAPHMRRVRITFSDHDHFREQWTKSENGKDTIFDL
                     HFVRR"
     gene            complement(2430..3125)
                     /locus_tag="VI195_05755"
     CDS             complement(2430..3125)
                     /locus_tag="VI195_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY6823924.1"
                     /translation="MKRHLLVLVSATILAASQGAVAQMMGHGNNSGAMGDWEFRIGPV
                     FLQSKNVDFKGGSNADIDSTTGIRVGTGYYVTDQLIVGMNFGWAESDFNGTVQGNLAT
                     SHIENGHVDFSTLMFDATYLLPLQGAIKPFATAGLGWNWINTNIASGPPQTGCWWDPW
                     WGYICSGYQPTHGSNSFAYQAGAGVQINFTRSFTINVDYRYTWIDINNANGTPGFGAV
                     GLMFVWRFPSYHY"
     gene            3306..3833
                     /locus_tag="VI195_05760"
     CDS             3306..3833
                     /locus_tag="VI195_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823925.1"
                     /translation="MSELSEWDSFYVIVGSAAGALVGLQFVVMTLIAEKPPVRAPELG
                     AAFATPTVIHFSTVLLLSAVVRAPWKAIAPLAALWGLVGLAGVAYSALVAWRVRRQTY
                     YRPVLEDWWFHVLLPLIAYAVLAVSALAAPLHERGALFGVGAAALLLLFIGIHNAWDT
                     VAYYVFVQMKGKDKD"
BASE COUNT          638 a         1253 c         1286 g          773 t
ORIGIN      
        1 ggatgtaggc cgccagcagg atgaacatga ggatggcggc aactttgatg gccgaaaacc
       61 aatactcgat ggcgccgaag accttcacgc tgcgtgcgtt cacgagcacc agcagcgcgg
      121 aaaatcccac gatccacggc caccccggcg agtgcggaaa ccaatagtgc atgtaaagcg
      181 cgatcgccgt aacctcggtg cccaccgcga gcacgatgct ggtccagtac gcgtagcgca
      241 cgagaaagcc ggcgagcggc ccgaggtaat actcggcata cgctccgaat gagcccgagg
      301 tcggatgcgc gacggtcatc tccgcgaggc agcccatgag cagcagcgca atcagtgcgc
      361 cgagcaggta gctcaacagc acggccggtc ccgccaggct gatcgcgaag ccgctgccga
      421 ggaacaaccc cgtgccgatg gcgccgccga tggcgatcat gctcatgcgc cgtgcggtga
      481 ggctgcggcg caagccgcgc tcgcgctcga tgatgtcgtc gaaactcgcg ccgcttccac
      541 gcatgtgtac ggtagggtaa ggctgcgccc atgtccgtgc taggctcgcg gctcggcggg
      601 tgcgtgggag gctgaccgtg ctcggctact ttcggctgct cacacgtccg cagctgcacg
      661 cgtatctggc ggcgctcggc ggctgggcgc tcgatgcgct cgacttcttc atcttcgtct
      721 tctgcctcaa ggcgatcagc ggcgagtttc acaccgatgt caaggcagtg agcgagggga
      781 tttttctcac cctcgcctgc cggccgctcg gagccctcgc cttcggttgg ctggcggaaa
      841 aatacggccg ccgtcccgtg ctgatgctca acgtggtcag ctactcgttg gtgcagctcg
      901 ccagcgcctt tgcgccaaac ctcgcgaccc tactggcgct gcgggcactg ttcggcttcg
      961 ccatgggtgg cgaatggggt gtcggcgcgg cgctggcgct cgagacgctg ccggcgaggg
     1021 gacgcggttt tttctccggg ctgctgcagg agggctacgt catcggctat ctgctggcct
     1081 cggtggtgtt cgcgacgctg ttcgagcggg ttggttggcg cgggctgttc atcgtcagct
     1141 cggtgtctgc gctgctcgtg ctatacatac gcttcggggt gcaggaatcg cccgcctggg
     1201 tcgcaggcgg tgcgccgcag cgagcctccg ccgtactgct gtggggcgcg gcgcgtgact
     1261 acttcccgac gctcgcatac ctggtgctgc tgatggcctg cttcaatgcc tactcgcacg
     1321 gctcgcagga cctgtatccc accttcctgc aggtgcaaca cggcttctcc gccgcgacga
     1381 cgggaaagat tgccatcgtc atgaacctcg gtgcactcgc cggcgggatc tgcttcgggt
     1441 cactgtccga gcggctcggc cgccgccggg cgatcgcgct ggcgacgctg ctgtcggtgc
     1501 cgatgattcc gctctgggcc tactcccata cggccgtcat gcttgcgctg ggcgcgttcc
     1561 tcatgcaatt catggtgcag ggtgcctggg ggatcgttcc ggctcatctc aacgagctgt
     1621 cacccccctc ggtgcgcgcc gtgctgccgg gtttcgccta tcagctgggc aacctcgcca
     1681 tggcgaagat ggcaccgatc cagtctggcc tcgcggaagc ccgtggcggg gactacgcct
     1741 acattctcgc ttccaccatg gtggtggtcg cggcggcact gatcatcgtg acattgttgg
     1801 gaccggagcg gcgttccgcg gagctgcggg tcgtcagctg aatgatacga tcgccgcgtg
     1861 cgtatccctg cactcgtatt agccgccgcg ctcgccgctg ccgcgcactg ccgcagcgcg
     1921 ccggcgcagg cgcccggccc ttatgaggcg ctggccgcgc tcgccggtga gtgggaggcg
     1981 actctgcagg gcttcggcaa gctgacgaat tccatccgcc tggtatcgaa gggaacggcg
     2041 atcgaggaga ccattggtac gcccgcggac aacgagacct cggtctacac tcgtgatggt
     2101 gcaagcgtgc tgctcacgca tttctgcgcc ctgacacccg cgggccacat cgcgcggctc
     2161 gcgctgcggg cgcggcaggg tacccaaccg ctcgagttcg tgttcgtcgg agcgagcaac
     2221 ctgccagagc tggatgcccc gcacatgcgg cgggtgcgca tcaccttctc cgaccacgat
     2281 catttccgcg agcagtggac caagagcgag aacggcaagg acacgatctt cgacctgcac
     2341 ttcgtgcgcc gctagcgcct ggaggcaaga gccgcgcacc ccgcgaaggg tgcgcgttgc
     2401 ctcagtgagt cggcccctga gtcggcgggt tagtagtgat aggaggggaa tctccacacg
     2461 aacatcagac cgacggcgcc gaagccgggg gtgccgttgg cgttgttgat gtcgatccag
     2521 gtatagcggt agtcgacatt gatggtgaat gagcgcgtga agttgatctg tacaccggca
     2581 ccggcctgat aggcgaagct gttcgagccg tgggttggct ggtaaccgct gcagatgtag
     2641 ccccaccatg gatcccacca gcagccggtc tgcggtggtc ccgaggcgat gttggtgttg
     2701 atccagttcc atcccagtcc ggcggtcgcg aagggtttga tggcaccctg cagcggcagc
     2761 aggtaggtgg catcgaacat cagcgtgctg aagtcgacgt gtccgttctc gatgtggctc
     2821 gtggcgaggt tgccttgcac cgtgccgttg aagtctgact cagcccagcc gaaattcatg
     2881 ccgacgatga gctggtcggt cacgtagtag ccagtgccga ccctgatgcc ggtggtactg
     2941 tcgatgtcag cattgctgcc gcccttgaag tcgacgttct tcgattgcag gaaaaccggc
     3001 ccgatgcgga actcccagtc gcccatggcg ccactgttgt tgccgtggcc catcatttgc
     3061 gcgaccgccc cttgcgaagc cgcgaggatg gtcgctgaaa ccaataccag gagatgacgc
     3121 ttcatatgca ctccaatcgc gctcagggcc tctgccccgc tacttcacat ggctttgcgc
     3181 tgcacagcaa tgcgactttg gggccatgcc gtccccgacc ggctccactg ttgcgtcgca
     3241 gcaacaaaca actgatccac tgctggtgac aatatcatcg tttgaaagtc acgcgaggcg
     3301 ctgtggtgtc cgagctctct gaatgggaca gcttctatgt catcgtcggt tccgcggccg
     3361 gagcactggt gggactgcag ttcgtggtca tgaccctcat cgccgagaag ccaccggtgc
     3421 gcgctcccga gctcggcgcc gcctttgcta ccccgacggt gattcatttc agcaccgttc
     3481 tgctgctgtc agcggtagta cgcgctccct ggaaagccat cgcaccgctc gcggccctgt
     3541 ggggactcgt tggcctcgca ggcgtggctt actccgccct ggtggcatgg cgggttcgcc
     3601 ggcaaaccta ctatcgtccg gtgctcgagg actggtggtt tcacgtgctg ttgccgctca
     3661 tcgcctatgc ggtgctcgct gtcagcgctc tggcagcgcc gctccatgag cgcggagctt
     3721 tgtttggcgt cggcgctgcg gcactgttgc tgctgttcat cggcatacac aacgcctggg
     3781 atacagtcgc ttactatgtc ttcgtccaaa tgaagggcaa agacaaggac tgagaggcgt
     3841 caaaggccca gatcgctcaa gccgggatgc tcatcgggcc ggcgaccgag cggccagtgg
     3901 aacttgcgct ccgccgcccg gatcggcaga tcgttgatgc tcgcgtagcg
//
LOCUS       DATDMP010000330         5989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_386128, whole genome shotgun sequence.
ACCESSION   DATDMP010000330 DATDMP010000000
VERSION     DATDMP010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386128"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..667)
                     /locus_tag="VI195_05765"
     CDS             complement(122..667)
                     /locus_tag="VI195_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY6823926.1"
                     /translation="MIDPAVGALLAGAFALLFASAALHKFLDAERFAGAFRAYEVVPP
                     PLAWVWRLVPILELVIALALLPAGSRRGAAAAGAALLLAYAGAIAINLGRGRRDLDCG
                     CGGPSERRPIGMWMVWRNVLLAALLAALRLPWADRPLSAVDALTIGAGTAMAALLYMS
                     LDTLFARPATQIARLRGGSGG"
     gene            735..2144
                     /locus_tag="VI195_05770"
     CDS             735..2144
                     /locus_tag="VI195_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6823927.1"
                     /translation="MSALPLPVSDFRALAARMTALTAEYLAGLDERRAFPHISGEQSH
                     ELFGETLPEEGLGPAALTALHQVLEFSRPPSPRFFGYVLGSGEPVAALADLLASVLNQ
                     NVTAWRSAPAAVALERGLIGALAAALGCPGYHGSFCGGGSMANLMALAIAREARLPAN
                     EDGARSGVVYASSEVHMSIPKAMALLGLGRRSLRVIPTDECWRMSLPHLRQAIATDRA
                     AGLEPLAVVATSGTVNTGSIDPLEQIAALCGENGLWMHIDGAYGALAALAAPERFAGL
                     NAADSISLDPHKWLYQPLDCGVLLFKDAAAARKAFAYSGDYAKPLQDDSLEGFAFFEE
                     SLELSRRFRALKLWLSLRYHGMRAFRHAIRADLRHAELLAKQIAASPRLELMAPVSLS
                     AVCFRYRTDTAGADEDRLNAALLKRVIERGSVYLSNATLGGHFALRACFVNHRTTEAD
                     VARIVPEVLAAAEEVLATR"
     gene            complement(2141..3826)
                     /locus_tag="VI195_05775"
     CDS             complement(2141..3826)
                     /locus_tag="VI195_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6823928.1"
                     /translation="MNNGERFIALRDELLHARTDWQGARHRFRWPVFEGFNWVGDYFD
                     VIAAGNDAAALRMVDDAGGDESLSFAAMAARAAQVAAFFAAQGLGRGDRLLVMLPNCI
                     ALWEVMLAAIRLGAVIIPATTLLEREELRDRLERGRVHAIVTEAALTARFAGLSGAPV
                     RIATGGSVKDWIPFAQSLQAPPSAPPAATRADELLLLYFTSGTTTVPKLVAHTHVSYP
                     VGHLSTMYWLGLKPGDVHLNLSSPGWAKHAWSSLFAPWNAQATILAYHYQRFDARALL
                     DKLVQCRVSTFCAPPTVWRMLIQSDLGNWPVTLREAASAGEPLNPEVIAQVREAWGIT
                     IRDGFGQTETTAQVGNSPAQPIKPGSMGLPLPGYPVVLLDTEGKPGEEGEICLDLSQR
                     PLGLMQSYLDDPQRTAIVMGGGHYHTGDVATRDADGYLTYVGRTDDIFKSSDYRISPF
                     ELESVLIEHPAVAEAAVVPSPDPLRLSVPKAFIVLRVGATADAATAVSILRHVRERVS
                     PFKRIRRVEFAALPKTISGKIRRVELRQLEQMRTADDARRALEFWEEDLRAHF"
     gene            3910..4485
                     /locus_tag="VI195_05780"
     CDS             3910..4485
                     /locus_tag="VI195_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY6823929.1"
                     /translation="MEPARLEAFTDGVVAIIITIMVLEIRVPHGTDLASLQAVLPVLL
                     AYVLSYVNVGIFWNNHHHMLHVTERVDGKVLWANLGLLFWLSLIPFVIRWIDESGFTS
                     LPTAAYGIVLTCAAVSYIILQRQIIAVNGPDSRLALALGGDLKGKLSLALYLAAIPLA
                     FARPWVAIMLYVAVALLWLVPDTRIEALVKK"
     gene            complement(4510..5097)
                     /locus_tag="VI195_05785"
     CDS             complement(4510..5097)
                     /locus_tag="VI195_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEY6823930.1"
                     /translation="MAVILRAADRRAVPWKDGGGVTREVAVHPPASDLGSFDWRVSLA
                     EVHQGGPFSTFPEIDRHLAVIAGRLELAIAGREVLTLSPQSAPVEFPGDLGASAQPVI
                     SPVSDLNVMTRRGHFVAHLRRRSAASPMMLRLEADTTLVLALAPLSLRTPSVAVQLAA
                     LDAVRFSVHPDDSCTASIEAAELDFWLIEIRAGAA"
     gene            complement(5088..5510)
                     /locus_tag="VI195_05790"
     CDS             complement(5088..5510)
                     /locus_tag="VI195_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6823931.1"
                     /translation="MSERLSRRTGGCLCGAVRYEVAGPLRPVVMCHCSQCRRLTGHIM
                     AATAARHTDFQLLAEESLGWYASSAEARRGFCKRCGSTLFWQGVGRDYLSIAAGTLDD
                     SSGLKIACHIFTADKGGYYEISDAAPQLRDGNFSLTWP"
     gene            complement(5507..>5989)
                     /locus_tag="VI195_05795"
     CDS             complement(5507..>5989)
                     /locus_tag="VI195_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6823932.1"
                     /translation="TTAAQLGIMGGSNGGLLVSASMVERPELFAAVVCQVPLIDMIRY
                     TQIGAGASWEAEYGDPGRPADRGWILKYSPYQNVSPGRKYPPVFFVTATSDDRVTPVH
                     ARKMAARMEAQGHEVLFYENTDGGHAAAADHKQAAEMWALSFVYLKQKLMRAAGATRP
                     "
BASE COUNT          993 a         2032 c         1989 g          975 t
ORIGIN      
        1 agcgcgccgg ctggggcgat gcgctcgtgc agaacgccaa cctggcgcgc aagcgcgagc
       61 acgacggcag cgagcaccag caccaacacc cacagcacca cgttggaaat cgccagcgcg
      121 ctcatccgcc gctcccaccg cgcaggcgtg cgatctgcgt cgctggacgt gcgaacaatg
      181 tatccaggct catataaagg agtgccgcca tcgccgtgcc cgcaccgatg gtgagtgcat
      241 ccacggcact cagcgggcga tccgcccatg gcaagcgcag cgcggcgagc agcgcggcga
      301 gcagcacatt gcgccacacc atccacatgc cgatcggacg gcgctcactt gggccgccgc
      361 acccgcagtc gaggtcgcgg cggccgcggc cgaggttgat ggcgatggcg ccggcgtagg
      421 caagcagcag cgccgccccg gcagcggcgg cgccgcgacg cgagcctgcc gggagcagcg
      481 cgagcgcgat gacgagctcg agtatcggca ccaggcgcca cacccaggcg agcggcggcg
      541 gcaccacctc gtacgcgcgg aacgcgccgg cgaagcgctc cgcatcgagg aatttgtgaa
      601 gcgcggcgct cgcgaacagc agggcaaacg cgccggcgag cagcgcacca acggccggat
      661 cgatcatgat gcgacatcat aaaccacccg ggcgtgtacc atcggcgcat cacccctgct
      721 gcaggactca ctgcatgtcc gcgctgccac tccccgtcag cgacttccgc gcgctcgccg
      781 cacgcatgac cgcgttgacc gccgagtacc tggccgggct cgatgagcgc cgcgcctttc
      841 cccacatctc cggtgagcag tcccacgagc tcttcggcga aaccctgccc gaagagggcc
      901 ttggacctgc cgccctgacg gctctgcatc aggtgctcga gttcagtcgc ccgccctccc
      961 cgcgcttttt cggctacgtg ctcgggtccg gtgagccggt cgccgcactg gcggacctgc
     1021 tcgccagcgt cctcaaccag aacgttacgg cgtggcgctc cgctccggcg gccgtggcac
     1081 tcgagcgcgg gttgatcggc gcgctggctg cggcgctcgg ctgcccgggc taccacggca
     1141 gcttctgcgg tggcggctcg atggcgaatc tcatggcgct cgcgatcgcg cgtgaggcgc
     1201 gcttgccggc gaacgaggac ggtgcccgtt ctggtgtggt gtacgcctcg agcgaagtgc
     1261 acatgtcgat accgaaggcg atggccttgc tcgggctcgg gcgcaggagc ctgagggtga
     1321 ttcccaccga tgagtgctgg cgcatgagtc tcccgcatct gcggcaggcc attgccaccg
     1381 accgcgccgc gggtctcgag ccccttgccg tcgtggcgac gagcggcacg gtcaacaccg
     1441 gcagcatcga tccgctcgag cagatcgccg cgctctgcgg cgagaacggg ttgtggatgc
     1501 acatcgatgg tgcctacggc gccctcgccg cgctcgcggc tccggagcgc ttcgcaggcc
     1561 tcaacgcagc cgactccatc tcgctcgatc ctcacaagtg gttgtaccag ccgctcgact
     1621 gcggagtgct gctgttcaag gatgccgcgg cggcgcgcaa ggccttcgcc tacagcggcg
     1681 actatgcgaa gccgctgcag gacgattcgc tcgagggctt cgcatttttc gaggaatcgc
     1741 tcgagctctc gcgacgtttc cgtgcgctga agttgtggct gtcgctgcgc tatcacggca
     1801 tgcgggcttt tcgccacgcg atccgcgcgg atctacggca tgcggagttg ctggcgaagc
     1861 agatcgcggc gagccctcgg ctcgagctca tggctccggt gagcttgagc gccgtgtgtt
     1921 tccgctaccg caccgacact gccggggcgg atgaggatcg gctgaacgct gcgctcctga
     1981 agcgcgtgat cgagcgcggc agcgtgtacc tctccaacgc gacacttggc gggcacttcg
     2041 cgctgcgcgc ctgtttcgtc aatcaccgca ccacggaggc ggatgtggcg cggatcgtgc
     2101 ccgaggtgct ggcggcagca gaggaggtgc tggcaacgcg ctaaaagtgc gcgcgcaggt
     2161 cctcctccca gaactccagc gcccgccgcg cgtcgtccgc cgtacgcatc tgctcgagct
     2221 gccgcagctc gacacgccgg atcttcccgg aaatcgtttt cggcagagcg gcgaactcca
     2281 cccgccggat gcgcttgaac ggggagacgc gctcgcgtac gtgacgcagg atcgagacgg
     2341 cggtggccgc atcggcggtc gcgcccaccc gaagcacgat gaaggccttg ggtaccgaga
     2401 gccgcagggg atcgggactt ggcaccacgg ccgcttcggc caccgcgggg tgctcgatga
     2461 gcacgctttc gagctcgaat gggctgatgc ggtagtcgga ggacttgaat atatcgtccg
     2521 tcctgccgac gtaggtgagg tagccatccg catcgcgcgt cgcgacatca ccggtgtgat
     2581 agtggccacc gcccatgacg atcgccgtgc gctgcgggtc gtcgagatag ctctgcatga
     2641 gaccaagtgg ccgctggctc agatcgagac agatctcgcc ctcctcaccc ggcttgccct
     2701 cggtgtcgag cagcacgact ggatagccgg gcaacggcag acccatcgag ccgggcttga
     2761 tgggctgcgc tggggaattc ccgacctggg cagtcgtctc ggtctgtccg aacccatcac
     2821 ggatggtgat gccccacgcc tcccgcacct gcgcgatcac ttcggggttg agaggctcgc
     2881 cggcactcgc ggcctcgcgc agtgttacgg gccagttgcc gagatcgctc tggatgagca
     2941 tgcgccacac cgtcggtggc gcgcagaagg tgctgacgcg gcactgcacg agcttatcga
     3001 gcagtgcgcg ggcatcgaag cgctgatagt gataggcgag aatcgtcgcc tgggcattcc
     3061 agggcgcgaa cagtgatgac caggcgtgtt tcgcccagcc gggcgagctg aggttcagat
     3121 gcacatctcc gggcttgagc ccgagccaat acatggtcga gaggtgcccg accgggtagc
     3181 tgacatgggt gtgcgcgacg agcttcggaa ccgtcgtcgt gccagaggtg aaatacagca
     3241 gcaacagctc atcggcgcgc gtcgccgccg gcggcgcgct cgggggcgcc tgcagcgact
     3301 gcgcaaaggg tatccagtcc ttgaccgaac cacccgtggc gatgcgcacc ggagccccgc
     3361 tgaggccggc gaagcgtgcg gtgagtgccg cctcggtgac gatagcgtgg acacggccgc
     3421 gctcgagtcg gtcgcgcagc tcctcgcgct cgagcaacgt ggtcgctggg atgatcaccg
     3481 caccgaggcg gatcgccgcg agcattactt cccacagggc gatgcagttg ggtagcatga
     3541 cgagcaatct gtcgccgcgc cctagtccct gtgccgcaaa aaacgcggcg acctgcgccg
     3601 cgcgcgcggc catggccgcg aatgatagcg actcatcgcc gccggcatcg tccaccattc
     3661 gcagcgccgc ggcgtcgttg ccggccgcga tcacatcgaa gtaatcgccg acccagttga
     3721 atccctcgaa caccggccag cggaaccggt ggcgcgctcc ttgccagtcg gtgcgtgcat
     3781 gcagcagctc atcgcgcagc gcaatgaacc gctcgccgtt gttcatggcc tcacctccgg
     3841 ccggcaccag gcaccgagct tagcatcgcc ctgatggctt gcgcgctcaa aggaacgtag
     3901 gatcctgcca tggagcctgc gcgcctcgaa gcgttcaccg acggggtcgt cgccatcatc
     3961 atcaccatca tggtgctcga gatccgcgtg ccgcatggca ccgatctcgc atcgctgcag
     4021 gcagttttac cggtgctgct ggcctacgtg ctgagctacg tcaacgtcgg cattttctgg
     4081 aacaaccatc accacatgct gcacgtcacc gagcgcgtcg acggcaaggt gctgtgggcg
     4141 aatctcggac tgttgttctg gctgtcgctc attccgttcg tcattcgttg gatcgatgaa
     4201 agcggattta cctcactgcc gactgcggcc tacggcatcg tgctgacctg cgcggcggtg
     4261 agttacatca tcctgcagcg ccagatcatt gccgtgaacg ggcccgactc gcgcctggcg
     4321 ctcgcgctcg gtggcgatct caagggcaag ctttccctcg cgctctatct cgccgcgatt
     4381 ccgctggctt tcgcccggcc gtgggtcgcc atcatgctat atgtggccgt ggcgctcctg
     4441 tggctcgtgc ccgacacccg catcgaagcg ctggtgaaga agtgagtcgc agcgattggg
     4501 aagttcgcat catgccgcgc cagcacggat ctcgatcagc cagaaatcga gttcggcagc
     4561 ctcgatcgaa gcggtgcagg aatcgtcggg gtgcaccgag aaccgcaccg catcgagcgc
     4621 tgccagttga accgcaacgg agggagtgcg caggctgagc ggtgcgagtg ccaggaccag
     4681 cgtcgtgtcc gcttccaggc ggagcatcat gggactggcc gcgctgcggc ggcgcaggtg
     4741 cgccacgaaa tggccgcgcc gtgtcatgac gttgaggtcg gacaccggcg atatcaccgg
     4801 ctgggcggac gcccccaggt cgccgggaaa ctcgactggc gcactctgcg gcgagagagt
     4861 caacacctcc cgaccggcga tcgcgagctc cagccgtccg gcgatcaccg cgaggtggcg
     4921 gtcgatctcc gggaaggtgg aaaaggggcc gccctgatgg acctcggcga gactcacccg
     4981 ccagtcgaag ctgccgaggt cgctcgccgg cggatgcaca gccacttcgc gagtcacgcc
     5041 gccgccatcc ttccagggca cggcgcgtcg atcggcggcg cgcagtatca cggccacgtg
     5101 agcgagaagt taccatcgcg cagctggggt gccgcgtcgc tgatctcgta gtagccaccc
     5161 ttgtccgcgg tgaaaatatg gcaggcgatt ttcagcccgc tcgaatcatc gagggtgccc
     5221 gcggcgatgc tcaggtagtc gcgccccaca ccctgccaga agagtgtcga gccgcagcgc
     5281 ttgcagaagc cgcgccgcgc ctcggcggaa gaggcatacc agccgaggct ctcttcggcg
     5341 agcagttgga agtcggtgtg acgtgccgcg gtcgcggcca tgatgtggcc ggtgagacgg
     5401 cggcactgcg agcagtgaca catgacgacc ggccgcagcg gacccgccac ttcatagcgc
     5461 actgccccgc acaggcagcc gccggtcctc ctcgacaagc gctcgctcac ggccgcgtgg
     5521 cgcccgcggc cctcatgagc ttctgcttga gatacacgaa gctcaaagcc cacatttccg
     5581 ccgcctgctt gtggtcggcg gccgccgcat ggccgccgtc ggtgttctcg tagaacagca
     5641 cctcgtgacc ctgcgcctcc atgcgcgccg ccatcttgcg tgcatgcacg ggcgtgacgc
     5701 gatcgtcgct ggttgcggtg acgaaaaaca ccggcggata tttcctgccg gggctgacgt
     5761 tctggtaggg cgaatacttg agaatccagc cccggtcggc cgggcgcccc ggatcaccgt
     5821 actcggcttc ccaggatgct ccggcgccga tctgcgtata gcggatcatg tcgatgagcg
     5881 gcacctggca gacgaccgca gcgaacagct ccggccgctc caccatgctc gcgctcacca
     5941 gcaggccgcc gttcgagccg cccatgatgc cgagctgcgc cgccgtggt
//
LOCUS       DATDMP010000331         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1607649, whole genome shotgun sequence.
ACCESSION   DATDMP010000331 DATDMP010000000
VERSION     DATDMP010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607649"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /locus_tag="VI195_05800"
     CDS             <1..1742
                     /locus_tag="VI195_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY6823933.1"
                     /translation="AQSATQPSDLLLLYANRQTADNVVRLAFQIARANAELLSSEASS
                     TQPSATTDHSPQSFAQLEQGMSQRRDALEKEMDTVRRQLARAPARTRSDLDAKLLELQ
                     GELAMVKAQKNLFDTMSQFVYENDVKRASVSALKARIDAIAATIPAATTAPATTAAAA
                     TSGSAAQPNAAGSAVPSSAAAGTAPSALGIWDLLAEALRLSQKLSTIDTVDQRTKALQ
                     ETFRKIRAAPSEQLQKLSARSDELAKIADSANGAALKQLRDQFDTLAWLFTQTSAIAL
                     PLSQEAVLLTQYRDNLDKWRASVKRQYHDVLGVLWTRIAVLAGALIAVFAGSELWRRT
                     VLRYVFEPRRRNQLLLIRRIVTWLLVLLILGLTFITHLSSFATFAGLLTAGVAVAMQS
                     VLVSVVGYFILIGKYGLRVGDRVQVGGVTGEVIDLGLIRMHLLELTSQEPLGPTGRVV
                     AFANSIVFQSAGGLFRQIPGVNFTWHELALSLPAGSDYPALKDRLLAAVTSVVEEYRG
                     EILRQAETIKQTTSLPATTDVAPQVHLQFSPSGVQAKVRYPVPLNRRAQVDERVSQEL
                     HQLLQSLETSRSA"
     gene            complement(1859..>2178)
                     /locus_tag="VI195_05805"
     CDS             complement(1859..>2178)
                     /locus_tag="VI195_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823934.1"
                     /translation="DAIANQGLHTVGYMLSLCDGYIRRFDPAQDDRDDEEVDFHSGRV
                     IYLTIGHIVGWVPSDATRLWRLAIERGEAQESRVRSGARASTHTNGKWSRYRTRVRHD
                     PTR"
BASE COUNT          378 a          730 c          687 g          383 t
ORIGIN      
        1 aggcgcagag cgccacgcaa ccgagcgatc tgctgctgct ttatgccaat cgccagaccg
       61 ccgataacgt ggtgcggctc gctttccaga tcgcgcgcgc caacgccgag ctgctgagca
      121 gcgaggcgag ctccacccag ccgagtgcca ccacggacca ttccccgcaa tcattcgcgc
      181 agctcgagca gggcatgagc caacggcgcg acgcgctcga gaaggaaatg gatacagtcc
      241 gccgccagct cgcgcgcgcg ccggcacgca cccggtccga tctcgatgcc aagctcctgg
      301 agttgcaggg tgagcttgcc atggtcaaag cgcagaagaa tctcttcgac accatgtcgc
      361 agttcgtcta cgagaacgac gtcaagcggg ccagcgtcag cgcactcaag gcgcgtatcg
      421 acgcgatcgc cgcgaccata ccggccgcaa cgacggcgcc cgccaccacc gccgctgcgg
      481 ccaccagcgg atctgcggcg cagccgaacg ccgctggcag cgcggtgccg agtagcgccg
      541 ctgccggcac ggcgccgagt gcgctcggta tctgggatct cctcgccgag gcgctcaggc
      601 tgtcacagaa gctgtccacc atcgatacgg ttgatcagcg caccaaggcg ttgcaggaga
      661 ctttccgcaa gatccgtgcc gcaccgagtg agcagctgca gaagctgtct gcgcgtagcg
      721 atgagctcgc caagatcgcg gacagcgcca acggcgcggc gctgaagcag ctgcgcgacc
      781 agttcgatac gctcgcctgg ctgttcacgc agacctcggc gatcgccctg ccgctcagcc
      841 aggaggccgt gctgcttacg cagtaccgcg acaatctcga caagtggcgc gcgtccgtca
      901 aacggcaata ccacgatgtg ctgggggtgt tgtggacacg catcgcggtg cttgccggcg
      961 cgctcattgc cgtgttcgcg gggtcggagc tctggcgccg taccgtgctc aggtacgtgt
     1021 tcgagccgcg gcgcaggaat cagctgctgt tgatacgcag gatcgtcacc tggctgctgg
     1081 tgctgctgat tctgggcctc accttcatca cccacctgag ctccttcgcc acttttgccg
     1141 ggctgctgac tgcgggcgtt gcggtggcga tgcagagcgt actggtgtcg gtggtgggct
     1201 atttcattct catcggcaaa tacgggctgc gggtcggcga tcgcgtgcag gtcggtggcg
     1261 tgacgggcga ggttatcgac ctgggactca ttcgcatgca tctgctcgag ctcacctcgc
     1321 aggagcccct cggaccgacc ggccgcgtcg ttgccttcgc caactccatt gtcttccagt
     1381 ctgccggtgg actcttcaga cagattcccg gagtgaactt cacctggcac gaactggcct
     1441 tgagcttgcc tgccggcagc gattaccccg ccttgaagga tcgcctgctc gcggcggtga
     1501 cctcagtcgt cgaggagtac cgcggcgaga tcctccgcca ggcggagacc atcaagcaaa
     1561 ccacctcgct ccctgcgacg accgacgtag caccgcaagt acacctgcag ttttccccgt
     1621 ccggggtaca agccaaggtg cgctatccgg tcccgctcaa ccgccgcgcg caggtcgatg
     1681 agcgcgtctc gcaggaactg catcagctgc tgcaaagtct cgagaccagc aggtcggcat
     1741 gaagcgcagt tcgtccgctg cgcaagctga gacgcggcat ctgcagcgcg ttgacagcac
     1801 tgcgggattc tattccgccg cggccccgaa ctgacgctcg agaaccgcgg cctcgggcct
     1861 acctggtcgg gtcgtggcgc acgcgagtgc ggtagcgcga ccatttgccg ttggtgtgag
     1921 ttgacgcgcg cgctccgctg cggacgcgcg attcctgcgc ttcgccgcgc tcgatcgcca
     1981 gccgccagag ccgcgtcgca tcgcttggca cccagccgac tatgtgaccg atggtcagat
     2041 agatcacacg accgctgtgg aaatcgacct cttcatcgtc gcgatcgtcc tgcgccggat
     2101 cgaagcggcg gatgtagcca tcgcataggc ttagcatgta gccgacggta tgcagtccct
     2161 ggttggcaat cgcatccg
//
LOCUS       DATDMP010000332         2283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1941441, whole genome shotgun sequence.
ACCESSION   DATDMP010000332 DATDMP010000000
VERSION     DATDMP010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941441"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VI195_05810"
     CDS             complement(<1..620)
                     /locus_tag="VI195_05810"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002341.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY6823935.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPERMARSQVATPSHLINTE
                     LYGELVKAGFRRSGIFTYRPHCDSCRACVPVRIPVREFIASRSQRRARALHAELTSQA
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLVEFRDNGQLVM
                     VSIVDYLPDGLSSVYTFYEPDRRGASFGTYNVLWQIDQCRRLGLPYL"
     gene            complement(607..1296)
                     /gene="aat"
                     /locus_tag="VI195_05815"
     CDS             complement(607..1296)
                     /gene="aat"
                     /locus_tag="VI195_05815"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY6823936.1"
                     /translation="MLAWLDRHDPFPPVEAALKDPNGLLCAGADLSVERLLAAYGSGI
                     FPWYSGDEPVLWWSPDPRMVLFCEELKVPRSLAKSIRNKGFEVRLDTCFEKVLVNCAR
                     ARKDGEGTWLGPAMRRSYLALHRAGYAHSFETWLGKELVGGLYGVSIGRMFYGESMFS
                     RASDASKVALVALVEELRARGFPLIDCQQRTPLLASLGAREIPRRQFLRRVAALVNYP
                     EQPGIWTRVSA"
     gene            complement(1304..1996)
                     /locus_tag="VI195_05820"
     CDS             complement(1304..1996)
                     /locus_tag="VI195_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY6823937.1"
                     /translation="MQRRDFIKFCAASTAAAGTPGLAADAAPNFYGRARLVDGGGAPL
                     KASRLPVDRNLIFHYPFSSTPCVLLNLGRPAKPSAQLATADKRSYEWSGGVGPRHSIV
                     AYSAICAHQLAYPTKDISFISYRTEKSARNRFGNVIHCCAEHSQYDPAEGARVLAGPA
                     PQPLAAILLEYDAASDGLTAVGTLGGEMFNQFFAKYEMKLTLEGARTRVPAESTCAVV
                     ELENYCRQQVKC"
     gene            complement(1999..>2283)
                     /locus_tag="VI195_05825"
     CDS             complement(1999..>2283)
                     /locus_tag="VI195_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY6823938.1"
                     /translation="DVTPVHGVGGVIGTLLTGVFASASLGGVGYPDNGSMGGQVMTQL
                     AGIVAVGAWSGIVTWILLKLTGAVTGMRVNPDQETEGLDTVEHNEKGYNL"
BASE COUNT          364 a          737 c          820 g          362 t
ORIGIN      
        1 aggtacggca gtccgaggcg ccggcactgg tcgatttgcc agagcacgtt gtaggtgccg
       61 aagctggcgc cgcggcggtc gggctcgtag aaggtataga ccgaggacag gccgtcgggc
      121 aggtagtcga cgatggacac catcaccagc tggccgttgt cgcggaactc gacgaggcgc
      181 gtgtccacgc ggctctgcag caggaagtgc gagtactggt cgcggctgtc gttgtccatg
      241 ccgccgccgg aatggcgctt ggactggtag cggagataaa gcgcgtagtg ctcgagccgg
      301 aacttgagcg gctgcgcctg cgaggtgagc tcggcatgca gcgcgcgcgc gcggcgctgc
      361 gagcggctgg cgatgaactc gcgcaccggg atgcgcaccg gcacgcaggc gcggcagctg
      421 tcgcagtgcg ggcggtaggt gaagatgccg ctgcggcgga agccggcctt gaccagctcg
      481 ccgtagagct cggtgttgat caggtggctc ggcgtggcga cctgcgagcg cgccatgcgc
      541 tcgggcaggt agctgcacgg gtagggcgcc gtgacgtaga actgcagtac cgcgtgcggt
      601 agatcgttaa gccgagacac gtgtccagat ccccggttgt tcgggatagt ttaccaatgc
      661 ggcgacacgg cgcaaaaact gccgccgcgg gatttcccgc gcccccagcg aggcgagcag
      721 cggggtgcgc tgctggcagt cgatgagcgg gaagccgcgc gcgcgcagct cctcgacgag
      781 cgcgaccagc gcgaccttcg aggcgtcgct ggcgcgcgag aacatcgact cgccgtagaa
      841 catgcggccg atcgacacgc cgtagagccc gcccaccagc tccttgccga gccaggtttc
      901 gaacgaatgg gcgtagcccg cgcggtgcag ggcgagatag gaccttcgca tggcggggcc
      961 gagccaggtt ccttccccgt ccttccttgc tctcgcgcag ttcaccagaa ccttctcgaa
     1021 acaagtgtcc aaccggacct cgaacccctt gttgcggatg ctcttggcga gagagcgcgg
     1081 gaccttcagc tcctcgcaga acagcaccat gcgcgggtcg ggcgaccacc acagcacggg
     1141 ctcgtcgccc gagtaccagg ggaagatgcc gctgccgtac gcggccagca ggcgctcgac
     1201 cgacaggtcg gcgcccgcgc agagcaggcc gttggggtcc ttgagggcgg cttcgacggg
     1261 cggaaaggga tcgtgccggt ccagccaggc tagcatcgcg ccgtcagcat ttgacctgtt
     1321 gccggcagta gttttccagc tccaccaccg cgcacgtgga ctcggccggc acgcgcgtgc
     1381 gcgcgccttc cagcgtgagc ttcatctcgt acttggcgaa gaactggttg aacatctcgc
     1441 cgccgagcgt gccgaccgcc gtgaggccgt cgctcgccgc gtcgtactcc agcaggatcg
     1501 ccgccagcgg ctgcggcgcc gggccggcca gcacgcgcgc gccttcggcg ggatcgtact
     1561 ggctgtgctc ggcgcagcag tggatcacgt tgccgaagcg gttgcgcgcg ctcttttcgg
     1621 tgcggtagct gatgaagctg atgtccttgg tcggataggc gagctggtgc gcgcagattg
     1681 ccgaataggc cacgatcgag tgccgcgggc ccacgccgcc ggaccactcg tagctgcgct
     1741 tgtccgcggt cgcgagctgc gccgaaggct tggctggccg gccgaggttc agcaggacgc
     1801 agggcgtgct ggagaacggg tagtggaaga tcaggttgcg gtcgaccggc aggcgggacg
     1861 ccttgagcgg ggccccgccg ccgtccacga gccgcgcgcg gccgtagaag ttcggcgctg
     1921 cgtcggctgc gaggcccggc gtgccggcgg cggcagtcga cgccgcgcag aacttgatga
     1981 aatcccggcg ctgcattcct acaaattgta gcctttctcg ttgtgctcga cggtgtccag
     2041 accctcggtt tcctggtcgg gattgacgcg catgccagtc accgcgccgg tgagcttgag
     2101 caggatccag gtgactatgc cggaccaggc gcctaccgcg acgatgcctg cgagttgcgt
     2161 catcacctgg ccgcccatgc tgccgttgtc cggatagccg acgccgccga gcgaggcaga
     2221 ggcgaacacg cccgtcagca gcgtgccgat cacgccgccc acgccgtgca ccggcgtgac
     2281 gtc
//
LOCUS       DATDMP010000333         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_942215, whole genome shotgun sequence.
ACCESSION   DATDMP010000333 DATDMP010000000
VERSION     DATDMP010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942215"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..>2179
                     /locus_tag="VI195_05830"
     CDS             126..>2179
                     /locus_tag="VI195_05830"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY6823939.1"
                     /translation="MGASPLRASAPDSAPHGAEGLAVLRGTEQVPGLHHVVRVQRDRW
                     GIAHIYARDQHDLFFAQGFVTAQDRLFQMELWKRAGEGRLAEILGPGAVQRDINARRL
                     KYRGDMTEEYRSYAADTLEILQAFTAGINAYITGLQRPGGPGLPLEFRVAGFRPEPWR
                     AEDCLNRLAAYAMMANASDELLHAQLVELVGPRKAAELVRFDPAAQLAPAPGIDLSGL
                     SPALLDNIVGSDRRIPFPATSLQESNNWTISGALTASGKPLLANDPHRVIAQPSLRYI
                     VHLVAPGWNVIGAVEPALPGVAAGHNQRIAWGFTIFGLDQQDLYIETLSPTDPKRYRT
                     EHGWALLHEEREVIHVRGAADVEAVLRFSAHGPIVWEDGKRALALRWAGAEPGTAGYL
                     GALSLDRARDWREFEAAMPRWKVPPENIVYADRAGNIGEHSTGLAPLRRNFNGLLPLP
                     ADGQFEWSGFIPNSALPHSYNPAAGFIATANQKTIPDDYPYAVGYEWSPPTRFARIQE
                     VLEQAKRAKHKLTVADMEALQLDVVSLLARDLQALLRRAVTPGSDPMSEAAELLLNWD
                     CALRADSPSAALYELWTLQLRRGVTRRALPQGAPNAMSTWSLYQVVLELSEPRDAVFG
                     AAAVSSRDALLRETLQAAYSELSTRQGSDPRRWSWGALHKAYFRHSLDDAPGLAGVLD
                     RGP"
BASE COUNT          358 a          732 c          719 g          370 t
ORIGIN      
        1 tgacggccat cactgcagct ctgccgcgcg ccgcacgcgc agcgcgacgg cagattttcc
       61 gatagtcttc atggatgttc agcccccgcg gcgcgcagct gctggcgatg atcgccgcca
      121 cggtcatggg cgcatcccct ctcagggctt ccgccccgga ttccgcgccg cacggcgccg
      181 agggcctcgc cgtcctccga gggaccgagc aggtcccagg cctgcaccat gtggtgcggg
      241 tgcagcgcga ccgctgggga atcgctcaca tctacgcgcg cgatcagcac gatctgttct
      301 tcgctcaggg tttcgtcact gcgcaggatc gcctctttca gatggagctg tggaagcgcg
      361 cgggagaggg acggctagcc gagatcctcg gtcccggtgc ggtccagcgc gacatcaacg
      421 ccagacgcct caagtaccgc ggtgacatga ccgaagagta ccgcagctac gctgccgata
      481 cgctggaaat cctgcaggca ttcacggctg ggatcaacgc ttacatcacc ggcctacaac
      541 gaccgggcgg tccggggctg ccccttgagt tccgcgtggc tggcttcagg cccgaaccct
      601 ggagagcgga agattgcctg aatcgcctgg cggcctacgc catgatggcg aacgcctccg
      661 atgagctgct gcatgcgcag ctcgtggagt tggtggggcc gcgcaaggcc gcggagttgg
      721 ttcgcttcga tccggctgcg cagctcgctc cggcgccggg gatcgacctc tccggcctgt
      781 cgcccgcgct gctcgataac atcgtgggct ccgatcggcg cattcctttt ccggcgacat
      841 cgctgcagga gagcaacaat tggaccatct cgggcgcact caccgcgagc ggcaagccgc
      901 tgctcgccaa cgatccccac cgggtgatcg cgcagccatc cctccgctac atcgtccacc
      961 tggtggcacc cggttggaac gtcatcggag cggtggagcc ggcgttgccc ggcgtggccg
     1021 ccggccacaa tcaacgcatc gcctggggtt ttacgatctt cggattggac caacaggacc
     1081 tgtacatcga gactctgagc ccgaccgatc cgaagcggta ccggaccgag cacggttggg
     1141 cgctattgca cgaggaacgc gaggtcatcc acgtgcgtgg cgcggcggat gtcgaggcgg
     1201 tgctgcgctt cagcgcacac ggcccgatcg tatgggagga cggcaagcgg gcgcttgcgc
     1261 tgcgctgggc cggagccgaa ccgggtacgg ccggatacct cggtgcactg agcctcgatc
     1321 gcgcccgcga ctggcgggaa ttcgaggccg cgatgccgcg ctggaaagtg ccgcccgaga
     1381 acatcgtgta cgccgatcgt gccggcaaca tcggcgagca ttcgaccggg ctggcgccgc
     1441 tgcgccggaa cttcaacggt ctgctgcccc tcccggcgga tgggcaattc gagtggtcgg
     1501 ggttcatccc gaactccgca ttgccgcaca gttacaaccc cgcggccggc ttcatcgcga
     1561 cagccaatca aaagaccatt ccggacgact acccttatgc ggtggggtac gagtggtctc
     1621 cgccgacccg cttcgcccgc atccaggaag tgctcgagca ggcaaagcgc gcgaaacaca
     1681 aactcaccgt cgccgacatg gaggccttgc aactggatgt ggtgtcactg ctcgcgcggg
     1741 atctgcaagc gctgctacgc cgcgcggtta cgcccgggag tgacccgatg agtgaggcgg
     1801 cggagctgct gctgaactgg gattgcgcct tgcgcgcaga ttcaccgagc gccgcgctgt
     1861 atgagctgtg gaccctgcaa ctgcgccggg gcgttacccg ccgcgcactc cctcaaggcg
     1921 cacccaacgc catgtcgaca tggtcgctgt atcaggtggt gctcgagctg tcggagccgc
     1981 gcgatgcggt gttcggtgct gcggccgttt cgagccgcga tgccctgctg cgcgagaccc
     2041 tacaggccgc ctatagcgag ttgagcacgc ggcaaggctc ggatccgcgc cgctggtcat
     2101 ggggcgcgct gcataaagcc tattttcgcc actcactcga tgatgctccg ggcctggccg
     2161 gtgtgctcga ccgcggacc
//
LOCUS       DATDMP010000334         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_497317, whole genome shotgun sequence.
ACCESSION   DATDMP010000334 DATDMP010000000
VERSION     DATDMP010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497317"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VI195_05835"
     CDS             <1..624
                     /locus_tag="VI195_05835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6823940.1"
                     /translation="QLALTLPNSREGESEADAVGTQLAAHAGYDPKAAAALWDKMAKL
                     GGAPPEFLSTHPSPEHRKERLEQLGQQVEPLYVAAREGRPTEAEVMTFRSVPFNQFKT
                     GTASFTCRLECAFSYNNKKAGWKKLHEQQNWRELALSVMSVGYLGDLSYFMLAESAKG
                     LGLADTAKVYYQRALQAGKEYGCAGGLSNTCEGFEVQKLASAALSRR"
     gene            complement(635..1216)
                     /locus_tag="VI195_05840"
     CDS             complement(635..1216)
                     /locus_tag="VI195_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY6823941.1"
                     /translation="MHVATHLLMGWTLAEHTTKTPRDRALVAWASVVPDLDGLGLLVD
                     AAAPFTGGTMQWYDRYHHMLLHGLPGAVLFTAILACFASDRLRTALLVLASYHLHLLG
                     DLLGSRGGAENALWPIHYLAPFSDAITLSWQGQWPLTSWQNTALTMALMAYAIALAIA
                     RGRSPVGLFSRRADEIFVQTLRHRFAKGPAASK"
     gene            complement(1230..>2179)
                     /locus_tag="VI195_05845"
     CDS             complement(1230..>2179)
                     /locus_tag="VI195_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY6823942.1"
                     /translation="PGGWLRYEARYSDRDGWYAPPAPEDLSVPASERKVLEQVARTLA
                     LEAAAPAQAVERLRQHFDRFTYSTWRDQDVPAGMTAMEDFLLHSKTGHCEYFAAATTL
                     LLRAAGIPARYATGYAVIEYSPLEEAWIVRARHAHAWSRAYINGRWTDVDLTPASWPE
                     AEAGLRPSWERLEDIVRWLVFRLTEGGENERSGAIALVALMLAALVGWRFLRKRKASR
                     PRTAAAEPQRPGEDSEFYQVERSLAARFPARSPHESLEQWCSGIEARLSLDMRSALRQ
                     LLELHYRYRFDPLGLDREARQALRARASVFQAELQRVSP"
BASE COUNT          390 a          712 c          744 g          333 t
ORIGIN      
        1 cagctcgcgc ttacgctgcc gaacagccgc gagggcgagt cggaagctga tgcggtcggc
       61 acgcagctcg ccgcgcatgc cggctatgac ccgaaggcgg ccgcggcgct gtgggacaag
      121 atggcgaagc tcggcggcgc gccgccggag tttctttcca ctcatccgtc ccccgagcac
      181 cgcaaggagc ggctcgagca gctcggccag caggtcgagc cgctctatgt cgccgcacgg
      241 gagggccggc caaccgaggc cgaggtcatg accttccgct cggtgccgtt caaccagttc
      301 aagaccggca cggcgagctt tacctgccgc ctggagtgcg ccttcagcta caacaacaag
      361 aaggccggct ggaagaagct ccacgagcag cagaactggc gcgagctcgc gctatcggtg
      421 atgagcgtcg gatatctcgg cgacctttcc tacttcatgc tcgccgagtc cgccaagggc
      481 ctcggcctcg ccgacaccgc gaaggtctac taccagcgcg ccctgcaggc cggcaaggag
      541 tacggctgcg ccggcgggct cagcaacacg tgcgaaggat tcgaagtcca gaagctcgct
      601 tcggctgcgc tcagtcgtcg ttgaagcacc cgctctactt gcttgctgcc ggtcctttgg
      661 cgaagcgatg cctcagggtc tggacgaaga tctcatcggc gcggcggctg aagagcccca
      721 cgggcgagcg cccgcgtgcg atggccaaag cgatcgcata cgccatcaac gccatcgtaa
      781 gggcggtgtt ttgccagctc gtcagcggcc attgtccctg ccatgaaagg gtgatcgcat
      841 cggagaaggg cgcaaggtag tggatgggcc acaaagcgtt ctctgccccg ccgcgcgagc
      901 cgagcaggtc gccgagcaag tgcaggtgat acgacgccag caccaacaat gcggtgcgca
      961 ggcgatcgct tgcgaagcag gcgaggatcg cggtgaagag cacggcgccc ggcagtccgt
     1021 gcagaagcat gtggtgatac cggtcgtacc actgcatcgt cccgccggta aaaggagctg
     1081 ccgcgtcgac gagcagcccc aggccatcga ggtcgggaac gacgctcgcc caggcgacca
     1141 atgcgcggtc ccggggcgtc ttcgtcgtgt gctccgcgag cgtccagccc atcaagagat
     1201 gcgttgccac gtgcatttgg agggctagct caaggagaaa cgcgttgcag ctcggcctgg
     1261 aacacggacg cgcgcgccct gagggcctgg cgggcttcgc gatccaggcc gagcggatcg
     1321 aaacggtacc gatagtggag ctcaaggagc tgcctcaacg ccgagcgcat gtcgagcgat
     1381 aaccgagctt cgatgcccga gcaccattgc tccagcgact catgcggcga gcgggcaggg
     1441 aagcgcgctg cgagcgagcg ctcgacctgg tagaactcgg aatcctcgcc gggccgctgc
     1501 ggctcggctg cggcggtccg cggcctcgaa gccttgcgct tgcgcaggaa gcgccagccg
     1561 acgagcgccg cgagcatgag cgcgaccagc gctatggcgc cggagcgctc gttttctccg
     1621 ccttcggtca ggcggaagac cagccagcgc acgatatcct cgagccgttc ccatgaaggc
     1681 ctgagacccg cttccgcttc gggccacgag gcaggcgtca ggtccacgtc ggtccagcgg
     1741 ccgttgatat acgcgcggct ccacgcatgg gcgtggcgcg cgcgcactat ccaggcttcc
     1801 tccagggggc tgtactcgat gacggcatag ccggtggcat agcgcgccgg gattcctgcc
     1861 gcgcgcagca ggagcgtggt ggcggcggcg aagtattcgc aatgcccggt tttcgaatgc
     1921 agcaggaagt cttccatcgc cgtcatgccg gcgggcacat cctggtcgcg ccaggtggaa
     1981 taagtgaagc ggtcgaaatg ctggcgcagt ctttcgaccg cttgcgccgg cgcggcggcc
     2041 tcgagcgcga gggtcctcgc gacctgctcg agcaccttgc gctccgaagc ggggaccgaa
     2101 agatcctcgg gagcgggagg cgcataccag ccatcccgat cgctgtagcg cgcctcgtag
     2161 cgaagccagc ctccgggtg
//
LOCUS       DATDMP010000335         4593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_608996, whole genome shotgun sequence.
ACCESSION   DATDMP010000335 DATDMP010000000
VERSION     DATDMP010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608996"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VI195_05850"
     CDS             <1..375
                     /locus_tag="VI195_05850"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY6823943.1"
                     /translation="AVSRAPLQKLQAQARRFGWTFKWVSSLGNDFNYDYNVSFPAESL
                     ERASACYNYSAQKLDSTEMPGISAFFRDGDQIYHSYSTYARGLDMLNAAYHYLDIAPK
                     GRDEDGLASPMAWVRHRIAYET"
     gene            402..992
                     /locus_tag="VI195_05855"
     CDS             402..992
                     /locus_tag="VI195_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY6823944.1"
                     /translation="MRYAEVHGKLADYRRQIAAIREKMRETLAGVEPQEVQDYQFASP
                     EGTVHLSQLFGQHEDLILIHNMGVSCSYCTLWADGYNGIHPHVITRAGFAVSSPDRPT
                     VQRKFAESRGWKFPLVSHAGTSFAADMGYVSAKGGWMPGVSVFRREGASVLRVSDTGF
                     SPGDDFCTLWHFFDLLPGGAGEWPSAVKKPVCSSSS"
     gene            complement(1143..2036)
                     /gene="rhtA"
                     /locus_tag="VI195_05860"
     CDS             complement(1143..2036)
                     /gene="rhtA"
                     /locus_tag="VI195_05860"
                     /inference="COORDINATES: protein motif:HMM:NF007823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HEY6823945.1"
                     /translation="MISVARSSRGSYLISIVLLLVAMGSIQIGASFAKTLFQSVGAAG
                     AVALRTALATFMLVAVLRPWRAKLGEGSWAALGAYGVSLGVMNLLYYLALRTLPLGIT
                     VSIEFAGPLAVAVLWSRRAIDFLWIGLAIAGLALLLPIGQGHGNVDPAGALFALGAGT
                     CWALYIVFGQKAGADYGPQTVALGSVIASIIVVPIGVAQAGARLLSPAVLPYGLAIAL
                     LSTALPYTLEMIALTSLPARTFGILMSIEPVFGALVGWALLHEQLSHVQWVAIAMIIL
                     ASVGTTWTAPAHPASPVPMPE"
     gene            complement(2033..3703)
                     /locus_tag="VI195_05865"
     CDS             complement(2033..3703)
                     /locus_tag="VI195_05865"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY6823946.1"
                     /translation="MAALLWLAVSVRPASAADYDLTIRNGHVIDGSGSPWYGADIGIK
                     DGRIAAIGRLGSESATRSIDAHGLVVAPGFIDMLGQSELTLLVDPRAPSKIFQGITTE
                     ITGEGESVAPLNELMLKENASTYARYGIKVDWHTLAQYFARLEKQGIGINFATYIGAT
                     SVREMVIGYANRAASADELREMQALVEQGVRQGAMGVSSALEYPPAPYASTEELIALA
                     RAAARFGGVYATHMRDEGNAEAAALQETLRIGREAQIPIEIFHLKVAAKPNWGTMPQL
                     VARIESARAQGIDVAADTYAYTAWSNSLTAFTPPWANDGGKEQLLRRLKEPAARARMR
                     KDMTTASGDWDNEWLEIEGPKDVLICAVNHEQLRRYQGKRLSEVAAEWHEDPIDTIFD
                     LLIKDDAATSVAVFGMSDPDVTLALQQPWVSIGSDDSATSPEGILGADHPHPRAYATF
                     PRILTKYVREEHRLSLPDAIRKFTALPAQREHLSDRGVIKLDMWADLVVFDPEAIRAV
                     ATYDNPNRLSQGMWYVLVNGVPVIEQGKMTGARPGKVLRGPGFTSHAP"
     gene            complement(3753..4046)
                     /locus_tag="VI195_05870"
     CDS             complement(3753..4046)
                     /locus_tag="VI195_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6823947.1"
                     /translation="MKATSRVQMENHWVKVTEWCFAPGAETGHHRHAYDYVVVPLTSG
                     VLRLEERAGRREAQLTSGVAYAREKGVEHNVVNVNDFEFRFLEVELLGPRTDR"
     gene            complement(4102..>4593)
                     /locus_tag="VI195_05875"
     CDS             complement(4102..>4593)
                     /locus_tag="VI195_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823948.1"
                     /translation="RVAVEGCVPKDRTIVVTITAPIRLASKTAAGMASSIRRRLARRA
                     AGGSIHRIHGNTIQIWILPRASAMTSKLVGFVHNPDANPRILIEATRALLAATGPQQR
                     AAARRSGPRWLVIADQDERLPIDTYRNICAQLRLQSVFAQIFVGVCGGRVERVAPPPA
                     ASG"
BASE COUNT          846 a         1502 c         1445 g          800 t
ORIGIN      
        1 gccgtctcac gagcgccgct gcagaagctg caggcccagg cgcggcgctt cggctggacc
       61 ttcaagtggg tctcctcgct cggcaacgat ttcaactacg actacaacgt ctcgtttcca
      121 gcggaatcgc tcgagcgcgc ctcggcctgc tacaactaca gcgcgcaaaa gctcgacagc
      181 accgagatgc cgggaatcag cgccttcttc cgcgacggcg accagatcta ccactcctat
      241 tcgacttatg cgcgtgggct cgacatgctc aacgcggcct accactacct cgacatcgca
      301 ccgaagggac gggatgagga cggactcgcc tcgccgatgg cgtgggtgag gcaccgcatc
      361 gcctacgaga cctgactgtg cccaagctgg agaggaccgt catgagatac gcagaggtgc
      421 atggcaagct cgccgattac cgccggcaga tcgccgcaat ccgggagaag atgcgcgaga
      481 cgctagccgg ggtcgagcct caggaggtgc aggactacca gttcgcgagc ccggagggca
      541 cggtgcacct ctcgcagctc ttcggccaac atgaggatct tatcctcatt cacaacatgg
      601 gcgtctcctg ctcctattgc acgctgtggg ccgatggcta caacggcatc catcctcacg
      661 tgatcacgcg tgcaggcttc gccgtgtcga gcccggatcg tcccacggtg cagaggaagt
      721 ttgccgagag tcggggctgg aaatttccgt tggtgagcca tgcgggcacc agcttcgccg
      781 cggacatggg ttacgtgtcc gcaaagggtg gctggatgcc gggggtctcg gtgttcaggc
      841 gcgaaggtgc gagcgtactg cgcgtttccg acacgggctt cagtcccggc gatgacttct
      901 gcacgctatg gcacttcttc gacctgctgc cgggcggcgc gggcgagtgg ccgtctgccg
      961 ttaagaagcc agtctgctcc agcagtagct gaacacggca gcagcgcgca tgtgtccgct
     1021 ttgcataggc tcggccctgt tggcagcgac cggagcgagc tcggcgggag gactcgcact
     1081 catcgccgcg cgcctgttgg ctgccggccg tccgcagccc cctgggcaag cgcagcgccc
     1141 gctcactcgg gcatcggcac cggtgatgcc ggatgggcgg gagccgtcca ggtagtgccg
     1201 acggaggcga gaatgatcat ggcgatcgcc acccactgaa cgtgggagag ctgctcgtgc
     1261 agcagtgccc agccgacgag tgcaccgaac accggctcga tgctcatgag gatgccgaag
     1321 gttcgtgcag gcaggctcgt aagtgcgatc atctccaggg tgtacggcag cgccgtcgac
     1381 aacagtgcga tcgcgagtcc gtacggcaac acggcgggcg atagcagccg cgcaccagcc
     1441 tgcgcgacgc caatgggcac gacaatgatc gaggcaatga cactgccgag ggcgacggtc
     1501 tgcggaccgt aatcggctcc ggccttctga ccgaacacga tatagagggc ccaacacgtg
     1561 cctgcgccga gagcgaacag tgcccccgcg ggatcgacgt tcccgtggcc ctggccgatc
     1621 ggcaacagca gcgccaggcc ggcaatcgcc agcccaatcc acaggaagtc gatcgcccgg
     1681 cgagaccaca gcacggcaac agccagcggg ccggcgaact cgatcgatac cgtaatgccc
     1741 agcggcagag tacgcagcgc caggtaataa agaaggttca tgacaccgag cgagacgcca
     1801 taggcgccca gtgcagccca cgatccctcg ccgagcttcg cgcgccaggg cctcagcacc
     1861 gcaacgagca tgaaggtggc gagcgcagtg cgcaatgcca ccgcgccggc agctccgaca
     1921 ctctgaaaca gcgttttcgc gaaggaagca cctatctgta tcgagcccat cgcgacgagc
     1981 aacaggacga tggagatgag ataagagccg cgcgagctac gcgcaacgga gatcatggag
     2041 cgtgggaggt gaacccgggc ccgcgtaaca ccttccccgg acgcgcgccg gtcatcttgc
     2101 cttgttcgat cacgggcaca ccgttgacga gcacgtacca catcccttgc gagagtcgat
     2161 tgggattgtc ataggtcgcc acggcccgaa tcgcctcggg atcgaacacc accagatcgg
     2221 cccacatgtc gagcttgatg acgccgcggt cggacagatg ctcgcgctgc gccggcagcg
     2281 ccgtaaactt gcgaatggcg tccggcagac tgagtctgtg ctcctcgcgc acatacttgg
     2341 tgaggatgcg cgggaaggtc gcataagcgc gcggatgcgg atggtcggca ccgagaatgc
     2401 cttccggaga agtggcgcta tcgtcgctgc cgatggatac ccagggctgt tgcagcgcca
     2461 acgtgacatc agggtcggac ataccgaata cagcgacgct ggtggccgcg tcatccttta
     2521 tcagcaagtc gaagatcgtg tcgatcggat cctcatgcca ctccgcagcg acttcactca
     2581 accgcttgcc ctggtagcgg cgcagttgct cgtggttgac ggcgcagatc agcacatcct
     2641 tgggaccctc gatttcgagc cattcgttgt cccagtcgcc tgaggcggtg gtcatgtcct
     2701 tgcgcattct cgcgcgcgcc gccggctcct tcaggcgtcg caggagttgc tcctttccgc
     2761 cgtcattcgc ccaaggcggc gtgaatgccg tcagcgagtt actccaggcc gtataggcgt
     2821 aagtgtcggc ggccacatcg atgccctggg cacgagcgct ctcgatccgc gcaaccagct
     2881 gcggcatggt gccccaattc ggtttggcgg cgaccttcag gtgaaagatc tcgatgggaa
     2941 tctgagcttc acgcccaatg cgcaacgtct cctgcagcgc tgccgcttcg gcattgcctt
     3001 catcacgcat gtgcgtggca taaacgccgc cgaaacgcgc cgccgcccgt gccagtgcga
     3061 tgagctcctc ggtgcttgca tacggtgcgg gcgggtactc cagtgcactg gagacgccca
     3121 tggctccctg acgcacgccc tgttccacca gggcctgcat ctcgcgcagc tcgtccgcgc
     3181 tcgcagcgcg gttggcgtag ccgatgacca tctcgcgcac cgaggtggct ccgatgtagg
     3241 tcgcgaaatt gatgccgatg ccctgctttt ccaggcgcgc gaaatactgc gccagggtat
     3301 gccagtcgac cttgatgccg tagcgtgcat aagtcgaggc gttctccttc agcatgagct
     3361 cgttgagcgg cgccaccgac tcaccttcgc cggtgatctc ggtggtgatg ccctgaaata
     3421 tcttggaagg tgcacgcggg tcgaccagca gcgtcagctc cgattggccg agcatgtcga
     3481 tgaaaccggg tgccaccacc agaccatgtg catcgatact gcgtgtcgcg ctctcgctcc
     3541 ccaacctgcc aatggcggca atgcggccat ccttgatgcc gatatccgcg ccataccagg
     3601 gcgaaccgct gccatcgatg acgtgcccgt tacggatggt gaggtcatag tccgccgcgc
     3661 ttgccggccg gactgacacc gcgagccaca gcagcgccgc catgccccac cgcaggcagc
     3721 cccacgcacc gctcatcggc gtcaggaaca tatcagcggt cggttcgggg cccgagcagc
     3781 tcgacttcga gaaagcgaaa ctcgaagtcg ttgacattca ccacgttgtg ctccacgccc
     3841 ttttcccgcg catacgccac gccgctggtg agctgtgcct cgcgccgccc cgcgcgctcc
     3901 tccaggcgca acacgccgct cgtcaacggc acgacgacat agtcgtacgc atgccggtgg
     3961 tgtccggttt ccgcgcccgg cgcgaagcac cactcggtta ccttgaccca gtgattttcc
     4021 atctgcacgc gcgatgttgc tttcatctgc tggcggctcc ggacgtccac gctggtggct
     4081 gctgcgctgc gcagggtaac attagccgct tgcggcgggc ggtggcgcaa ccctttcgac
     4141 ccgtccgccg caaacgccca cgaagatctg agcgaaaacg ctttgcaggc gcagttgtgc
     4201 gcagatattg cgataggtgt cgatcggcag acgctcatcc tgatctgcga tgaccagcca
     4261 gcgcggcccg gaccggcgag ctgcggcgcg ctgctgcggg ccggtggctg cgagcaaggc
     4321 acgagtcgcc tcgatgagaa tcctggggtt cgcatccggg ttatggacga acccgaccag
     4381 cttcgatgtc atggcgctag cgcgcggcaa gatccaaatc tgaatggtgt tgccatggat
     4441 gcgatggatg ctgccccccg ccgccctgcg cgcgagccgg cggcggatgc tgcttgccat
     4501 gccggctgcc gtcttggacg ccagccgtat cggagcagtg atggtcacga cgatcgtgcg
     4561 gtctttcggc acacagcctt caactgccac gcg
//
LOCUS       DATDMP010000336         2023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1053357, whole genome shotgun sequence.
ACCESSION   DATDMP010000336 DATDMP010000000
VERSION     DATDMP010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053357"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1032
                     /locus_tag="VI195_05880"
     CDS             106..1032
                     /locus_tag="VI195_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY6823949.1"
                     /translation="MSETNTVSPLRQRMIEDMAARKLNPHTQRSHISSCKRFAAWLKR
                     SPNTATPDEVRRFQLYLIESGASICNRNRIMTGVRFLFRVTLRRHDLAAEVWHIKEPQ
                     KLPPVLSPEEVKRVLTMATSLKARAMLTLAYGCGLRAGEVVRLRAGDIDSEQMIIRIV
                     QSKGRKDRHVMLPAEILELLRQWWKARPTEYNAGVAPEQRWLFPGRSEHRPLTTRQFS
                     RLFKEAAKAAGLRKTLSLHTLRHSFATHLLERGTDIRVIQALLGHDKLETTARYTRVA
                     IGMIAKIESPLDGLSAPRRRRARLDTEEPPAP"
     gene            1044..>2023
                     /locus_tag="VI195_05885"
     CDS             1044..>2023
                     /locus_tag="VI195_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEY6823950.1"
                     /translation="MARPALEVADILRDHGPAWREANRGRVSLDQLKVMSAIERCRTV
                     ALGGHVARCENEACGHTLVAYNSCRNRHCPKCQGAASRRWLADREAELLPVQYFHVVY
                     TLPSELRDIAYQNKRVIYDLLMKAAAETTLAIATDPKRLGARIGITAVLHTWGSALTH
                     HPHVHMIVPGGGLSADGARWVGSRSNFLVHVNVLARLFRGKMLAMLMDAHDAGQLTFF
                     NTHAGLADKRTFKRFIAALRHIKWVVYCKAPFAGPGQVLRYLSRYTHRVAISNRHLVA
                     ADDAGVSFRWKDYRIEGPGRWKTMTLTPHEFIRRFLMHVLPKGFHRIRHYG"
BASE COUNT          365 a          647 c          672 g          339 t
ORIGIN      
        1 aggagccgag aggccggttg ccagtcgaga ggacaccaat agcaggtgga gagccccgtc
       61 ctgagaatcg aggtgtcgtc aacctcactc aggatggagc tctccatgag cgagactaac
      121 actgtcagtc cgctgcgtca gcgtatgatc gaagacatgg ctgcgcgcaa gctcaacccg
      181 cacacgcagc gcagccacat ctcgagctgc aagcggttcg ccgcttggct caagcgctcg
      241 cccaatactg caacgcccga cgaggtgcgc cggtttcagc tgtacctcat cgagagcggg
      301 gcgagcatct gtaaccgcaa tcggatcatg accggggtgc ggtttttgtt ccgcgtcacg
      361 ctgcgtcgcc acgatctggc ggccgaggtc tggcacatca aggagccgca gaagctgccg
      421 ccggtgctga gccctgagga ggtcaagcgt gtcctgacga tggcgacgag cctgaaggcg
      481 cgagcgatgc tgacactcgc ctatggttgc gggctgcggg ccggcgaggt ggtgcggctg
      541 cgggcgggcg acatcgacag tgagcagatg atcatccgca tcgtgcagtc gaagggccgc
      601 aaggaccggc acgtgatgct gccggcggag atcctcgagc tgttgcggca atggtggaag
      661 gcgcggccga ccgagtacaa cgccggtgtc gcgccggaac aacgctggct gtttcccggt
      721 cgcagtgagc atcggccgct gaccacgcgc cagttcagcc ggctgttcaa ggaagcggcg
      781 aaagccgcgg ggctgcgcaa gacactctct ctgcatacgc ttcgccatag tttcgcgacc
      841 catctgctcg agcgcggcac ggacattcgg gtgattcagg cgttgctcgg acacgacaag
      901 ctcgagacga cggcccgcta cacccgcgtg gcgatcggaa tgatcgccaa gatcgagagc
      961 ccactcgatg gcctgagcgc gccgcgccgc aggcgcgcga gactagacac cgaggagccg
     1021 ccggcaccgt agcggcgccg gccatggctc gaccagctct ggaggtcgcg gacatcctcc
     1081 gcgatcacgg ccctgcttgg cgtgaggcca accgcgggcg tgtaagcctc gaccaattga
     1141 aggtgatgag tgcgatcgag cgctgtcgca cggtggcgct cggcgggcac gtcgcacgct
     1201 gcgagaacga ggcgtgcggc catacgctgg tcgcgtacaa ctcctgccgc aaccggcatt
     1261 gcccgaagtg ccagggcgcg gcgtcacgcc gatggctggc cgaccgcgaa gcggagctct
     1321 tgccggtgca gtactttcac gtcgtgtaca cgctgccgag cgagctgcgt gacatcgcgt
     1381 accagaacaa gcgcgtgatc tacgacctgc tgatgaaggc ggcggccgag acgacgctcg
     1441 ctatcgcaac cgatcccaag cgcctcggcg cgcggatcgg tatcacggcc gtgctgcaca
     1501 cgtggggctc ggcgctcacc catcatccgc acgtgcatat gattgtgccg ggtggagggc
     1561 tctcggccga tggcgcgcga tgggtcggct cacgctccaa cttcctcgtg catgtcaatg
     1621 tgctggcgcg cctgttccgc ggcaagatgc tggcgatgct catggatgcg cacgacgccg
     1681 gccagttgac gttcttcaac acgcatgccg gactcgccga caagaggacg ttcaagcgct
     1741 tcatcgccgc gctgcggcac atcaagtggg tggtgtattg caaggcaccg ttcgcagggc
     1801 ccgggcaggt gctgcgctat ctctcccgtt atacgcatcg tgtcgccatc tccaaccgcc
     1861 acctcgtcgc cgccgatgat gccggcgtgt cgttccgctg gaaggactat cgcatcgaag
     1921 gtcctggccg gtggaagacg atgacgctga cgccgcacga gttcattcgc cgcttcctga
     1981 tgcacgtgct gcccaagggc ttccatcgca tccgccacta cgg
//
LOCUS       DATDMP010000337         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_831410, whole genome shotgun sequence.
ACCESSION   DATDMP010000337 DATDMP010000000
VERSION     DATDMP010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831410"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VI195_05890"
     CDS             complement(<1..305)
                     /locus_tag="VI195_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY6823951.1"
                     /translation="MLLAPARLGYALWLLLTFVAVGVSALLLLIVLPGVERRRVAARA
                     AARSFLRLAGLPLVVRFPERLPPGQCVVVCNHASYLDGIVLTAALPPRFAFVIKREMA
                     "
     gene            567..1472
                     /gene="xerD"
                     /locus_tag="VI195_05895"
     CDS             567..1472
                     /gene="xerD"
                     /locus_tag="VI195_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY6823952.1"
                     /translation="MTANHDSADPAVSRFLDAVWMERGLSPNTLAAYRADLTALARWL
                     AERNVPIMRTTRADLQDFIAWRVRAGARPRSTARQLSSFRRFFRYFMREGAIREDPTA
                     QIAMPKIGRSLPRSLTEEEVEALLSAPVVSDPLGYRDRTMLEVLYATGLRVSELVNLR
                     QGQINLNQGVIRILGKGDRERLIPLGEEAMRALKEFSRGARGEILLERQTDYLFPTRR
                     GDRMTRQAFWHIIKRYARKAGINKELSPHTLRHAFATHLLNHGADLRVVQMLLGHSDL
                     STTQIYTHVARERLKELHAQHHPRG"
     gene            1604..2329
                     /locus_tag="VI195_05900"
     CDS             1604..2329
                     /locus_tag="VI195_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY6823953.1"
                     /translation="MIAHRLLVLLALCCAAGTVPAQQAPNKADPRAEIAARIPGTRPE
                     ELRATAVAGIYELTRGADIVYVTADGKYAFTGDLVELASNSNLTEQHRRELRAKAVAT
                     FPEDAMLIFGPKDPKYTVTVFTDVDCPYCRKLHSQIGDYNRLGIRVRYLLYPRTGPNT
                     QSWTKAEQVWCSPDRNAALTLAKLGQELKSKPCANNPVARSYALGQDFALEGTPAIIM
                     PNGELLPGYVPPDVLAQHLKDAK"
     gene            complement(2358..2681)
                     /gene="fdxA"
                     /locus_tag="VI195_05905"
     CDS             complement(2358..2681)
                     /gene="fdxA"
                     /locus_tag="VI195_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY6823954.1"
                     /translation="MTFVVTETCIKCKYMDCVEVCPVDCFHEGPNFLAIDPEECIDCT
                     LCEPECPAEAIFSEDEVPKGQEQFLKLNAELAKQWPVITEKKDAPPDAKEWEGKPDKL
                     KLLER"
BASE COUNT          471 a          954 c          877 g          438 t
ORIGIN      
        1 gccatctcgc gcttgatcac gaaggcgaaa cgcggcggca gcgcggccgt cagcacgatg
       61 ccatcgaggt agctggcgtg attgcacacc accacgcact gacccggcgg cagtcgctcc
      121 gggaacctga cgactagcgg cagcccggcg agccgcagga agctgcgtgc cgcggcacgc
      181 gccgccaccc gccgccgctc gactccgggc agcacgatga gcagcagcag cgcgcttacc
      241 ccgaccgcca caaacgtgag cagtagccag agcgcgtagc cgaggcgcgc cggggccagg
      301 agcaacgaac ggctgagcga tggagcgttc aaatcggcat caggtggcag gcggcgggtc
      361 gctggcggct gtggagtgtg aacaggttct ctttatgtta atgctggagc gcagatgtga
      421 aggtggtcac gtcgatggca gggtcgcgct caccatactg gggtaccaca ttatccggaa
      481 ggccgggccc ataggcggct gaagatagcg cgaccgttga gctcagctct ccagcgagac
      541 caggtttgtc gaataccgcc attgccgtga ctgccaacca cgattccgcc gatcccgccg
      601 tatctcgctt ccttgatgcg gtgtggatgg aacggggcct gtcgcccaac accctggcgg
      661 cctaccgcgc cgacctgacg gcgctcgcgc gttggctcgc cgagcgcaac gtgccgatca
      721 tgcgcacgac gcgcgcggac ctgcaggatt tcatcgcctg gcgtgtgcgc gccggcgccc
      781 ggccgcgctc caccgcacgg cagctctcca gcttccggcg cttcttccgt tacttcatgc
      841 gcgagggtgc catccgcgag gatcccaccg cacagatcgc gatgccgaag atcggccgct
      901 cgctgccgcg ctcgctcacc gaggaggagg tcgaggcgct gctgtcggcg ccggtggtga
      961 gcgacccgct cggctaccgc gaccgcacca tgctcgaggt gctgtatgcg accgggctgc
     1021 gcgtctcgga gctggtcaac ctgcgccagg ggcagatcaa cctcaaccag ggcgtgatcc
     1081 gcattctcgg caagggcgat cgcgagcggc tcattccgct cggcgaggag gcgatgcgcg
     1141 cgctcaagga gttcagccgc ggcgcgcgtg gcgagatcct gctcgagcgt cagaccgact
     1201 atctgttccc gacgcgccgc ggggatcgca tgacgcgtca ggccttctgg cacatcatca
     1261 agcgttacgc gcgcaaggcc ggtatcaaca aggagctctc gccgcacacc ctgcggcacg
     1321 ccttcgcgac gcacctgctc aaccacggcg ccgacctgcg cgtggtgcag atgctgctcg
     1381 ggcacagcga tctctcgaca acgcagatct acacgcacgt ggcacgcgag cggctcaagg
     1441 agctgcacgc ccagcatcac ccgcgcggct gaaaccgcag gaacgccgcc gccgacggtg
     1501 cttgcgccgc gtgctaaact cgcttcgccg acctgcgctg tgccgcagcc gaacctcgtg
     1561 cggccgtggc ggtccaaact cccttgagcg gcggaagtca ttcatgattg cccacaggtt
     1621 gctagttttg ctcgcgctgt gctgcgcggc tggtacggtg ccggcgcagc aggcgcccaa
     1681 caaggcggat ccgcgcgccg agatcgccgc ccgcatcccc gggacacgtc ccgaggagct
     1741 gcgcgccacc gcggtggcgg ggatctacga gctcacccgc ggtgccgata tcgtgtacgt
     1801 gaccgccgac ggcaagtatg ccttcacggg cgatctcgtc gagcttgcga gtaacagcaa
     1861 cctcaccgag cagcaccggc gcgagctgcg tgccaaggcg gtcgccacct ttcccgagga
     1921 cgcgatgctg atcttcggtc ccaaggaccc caagtacacc gttacggtat tcaccgacgt
     1981 ggactgcccc tactgccgca agctgcacag tcagatcggc gactacaacc gcctcggcat
     2041 ccgcgtgcgt tacctgctgt acccgcgtac cggccccaac acccagtcgt ggaccaaggc
     2101 ggagcaggtg tggtgctcgc ccgaccgcaa cgcggcgctc accctggcca agcttggcca
     2161 ggagctcaag tccaagccct gcgccaacaa ccccgtggca cgcagctatg cgctcggtca
     2221 ggatttcgct ctggaaggga cccccgccat catcatgccg aacggcgagc tgctgcccgg
     2281 gtacgtaccg cccgatgtgc tggcgcagca cctgaaggac gccaagtagc gggacttcgc
     2341 ggctgcttcg gcgcggctca gcgctcgagc agtttcagct tgtcgggctt gccttcccac
     2401 tccttggcgt cgggcggcgc atccttcttt tcggtgatca ccggccactg cttggcgagc
     2461 tcggcgttca gcttcaggaa ctgctcctgg cccttcggca cctcgtcctc ggagaagatc
     2521 gcctcggccg ggcactccgg ctcgcacagc gtgcagtcga tgcactcctc cgggtcaatg
     2581 gcgagaaagt tcggcccctc gtggaagcag tccaccgggc acacctcgac acaatccatg
     2641 tacttgcact tgatgcaggt ttcggtaacg acaaaggtca ttcgcaatcc tcgggaatcc
     2701 gcggggccgc aaagacgccg cgtgcgcggc aaggcggacc
//
LOCUS       DATDMP010000338         3059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_53844, whole genome shotgun sequence.
ACCESSION   DATDMP010000338 DATDMP010000000
VERSION     DATDMP010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53844"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VI195_05910"
     CDS             complement(<1..661)
                     /locus_tag="VI195_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823955.1"
                     /translation="MSGESLESLLKRSGNTVELLRNSQVGAYVYPIVPSEFSNWRSEQ
                     VGWQKTAVLYDQSHHMSEVTVSGRDALELVTHLTINSFANFAPNRAKQMVPCSYDGYV
                     IGDGILFYLDKDQLLFVGRTPTVNWIEFHAHTAGYKVETIRDERSPSNPRGKAVLRRH
                     YRYQIQGPKAEQVLEKLNGGPLPQVKFFHMDAINIKGRKVRCLRHGMAGAPGLEIWGP
                     YA"
     gene            complement(684..1955)
                     /locus_tag="VI195_05915"
     CDS             complement(684..1955)
                     /locus_tag="VI195_05915"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: protein motif:HMM:NF009904.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HEY6823956.1"
                     /translation="MASIVGGIGSSHTPTIGFALDAHKQSDPVWAPIFAGYEPVQKWL
                     REKRPDVLFFIYNDHVTSFFFDHYSHFALGVGAEYAVADEGGGPRQLPPVKGHPALAR
                     HIAMALTAEEFDLAYFQGKPLDHGCFSPLSVMWPHADGWPGAIVPLQVGVLEFPTPTA
                     RRCFKLGQSLRRAIQSYPEDLKVAIVATGGLAHQVHGERAGFNNTPWDMEFLELLERE
                     PARLAELTIAQYAERGGLEGAEVIMWLIMRGALSPRVRKLHSTYYLPSMTPIVTVIYE
                     DDTPVAATESDADFRARIARELAGIEKLPGTYPFTLDRSVKAYRLNSFLHRLIEPEFR
                     QRFLADPEPLFAAAELTAQERDLVRRRDWRALTHYGVIFFLLEKLAAVLGITNLHVYA
                     AMRGETLEEFQKTRNVKMLYSVAGQGAQLKQ"
     gene            complement(1995..2546)
                     /locus_tag="VI195_05920"
     CDS             complement(1995..2546)
                     /locus_tag="VI195_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEY6823957.1"
                     /translation="MSAPSAPQGIKAPPSTAYKIVDIPETYRDVIQRYAVPGTFVSAE
                     EKDSPWVPFGTNAAIRHLAFDVRHNIFSNILWIKSKGVVGTHKHRGTVVMICLEGSVR
                     YLEYDWVATPGAFIYETPGLTHTLVTDDPKGVKLFGWLQGPLEWYDDKGNFVETSDVW
                     WFMNHYESYCREHGIPLNKQLYL"
     gene            complement(2670..>3059)
                     /locus_tag="VI195_05925"
     CDS             complement(2670..>3059)
                     /locus_tag="VI195_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823958.1"
                     /translation="CAFQPPGVTALDARLPKTPKWKGSIWPSYDYILPSQSTLRLLAA
                     FTYTGEIWNDALNTPELRRPASRQLDASIHYISPGAKYDFAFGGTNLTNDRYCTAGSP
                     NAGAGEVGCYYNAPLMWYVSLRAQFGR"
BASE COUNT          528 a          983 c          980 g          568 t
ORIGIN      
        1 cggcgtacgg accccagatc tccaggcccg gcgccccggc catgccgtgc cgcagacagc
       61 gcaccttgcg acccttgatg ttgatcgcat ccatgtggaa gaacttcacc tgcggcagcg
      121 gcccgccgtt gagcttctcg agcacctgct cggccttcgg cccctggatc tgatagcggt
      181 agtgccggcg cagcaccgcc ttgccacggg ggttggaggg cgagcgctcg tcgcgaatgg
      241 tctcgacctt gtagccggcg gtgtgcgcgt gaaactcgat ccagttgacg gtgggcgtgc
      301 gcccgacgaa cagcaactgg tccttgtcga gatagaagag gataccgtcg ccaatcacgt
      361 agccgtcgta gctgcaaggc accatctgct tcgccctgtt cggcgcgaag ttggcaaagc
      421 tgttgatcgt gagatgtgtt accagctcga gggcgtcgcg cccgctgacc gtcacctccg
      481 acatatggtg cgattgatcg tagagcacgg cggttttctg ccagccgacc tgctcgctgc
      541 gccagttgct gaactcgctc ggcacgatcg gatagacgta cgcccccacc tgcgagttgc
      601 gcagcagctc gaccgtgttg ccggaacgtt tcagcagact ctccagactc tcgccactca
      661 tcgctgcaat tccccaccgg cgttcattgt ttgagctgcg caccctggcc ggcgaccgag
      721 tagagcatct tcacgttgcg ggtcttctgg aactcctcca gcgtctcgcc gcgcatcgcg
      781 gcatagacgt gcagattggt gatgccgagc accgcagcca gcttctccag cagaaagaag
      841 atcacgccgt agtgggtgag cgcgcgccag tcgcgccgcc gcaccaggtc gcgctcctgc
      901 gcggtcagct ccgctgccgc gaacaacggc tcgggatcgg cgaggaagcg ctgccggaac
      961 tccggctcga tcaggcggtg cagaaagctg ttgaggcggt acgccttcac gctgcggtcg
     1021 agcgtaaatg gataagtgcc gggcagcttt tcgattccgg cgagctcccg ggcgatgcgc
     1081 gcgcggaaat ccgcatcgga ctccgtcgcg gccacgggcg tgtcgtcttc atagatcacc
     1141 gtgacgatcg gagtcatcga tggcaggtag taggtgctgt ggagcttgcg cacccggggc
     1201 gacagcgcgc cgcgcatgat cagccacatg atgacttcag cgccttcgag cccgccgcgc
     1261 tccgcatatt gcgcgatcgt cagctcggcg agtcgcgcgg gctcgcgctc gagcagctcg
     1321 aggaactcca tgtcccaggg ggtgttgttg aagccggcgc gctcgccatg tacctgatgc
     1381 gcgagtccgc cagtggcgac gatggccacc ttgagatcct ccgggtagct ctggatcgcg
     1441 cggcgcaggg actggccgag tttgaaacag cgccgcgccg tcggcgtggg aaattccagc
     1501 acgcctacct gcagcgggac gatggcaccc ggccagccgt ccgcgtgcgg ccacatcact
     1561 gacagcggcg agaagcagcc gtgatcgaga ggcttcccct ggaagtaggc gaggtcgaac
     1621 tcctctgcgg tcagcgccat ggcgatgtgg cgcgcgagtg ccgggtgccc cttgaccggc
     1681 ggcagttgcc gcggcccgcc accttcgtcg gcgacggcgt actccgcacc gaccccgagc
     1741 gcgaagtgcg agtagtgatc gaagaagaac gaggtgacgt ggtcgttgta gatgaagaac
     1801 aggacgtcag gccgcttttc ccgcagccac ttctgcaccg gctcgtagcc cgcgaagatc
     1861 ggcgcccaca ccggatcgct ctgcttgtgc gcatccagcg caaacccgat ggtcggggtg
     1921 tgcgaggaac cgatcccgcc cacgatgctg gccatgttca accactctcg aaggccccgg
     1981 ggcggggcct gcgctcacag atacagctgc ttgttgagcg gaatgccgtg ctcgcgacag
     2041 tagctctcgt agtggttcat gaaccaccac acatccgagg tctcgacgaa attgcccttg
     2101 tcgtcatacc actccagcgg tccctgcagc cagccgaaga gcttcacgcc cttcgggtca
     2161 tccgtcacga gcgtgtgcgt gagcccgggt gtctcgtaga tgaacgctcc aggggtcgcc
     2221 acccagtcgt attcgaggta gcgcacgctt ccctcgaggc agatcatcac cacggtgccg
     2281 cgatgcttgt gcgtgcccac cacgcctttg gatttgatcc acaggatgtt gctgaagatg
     2341 ttgtgacgca cgtcgaacgc aaggtggcgg atcgccgcgt tggtgccgaa cggcacccag
     2401 gggctgtcct tctcctcggc actgacgaag gttccgggca cggcatagcg ctgaatgaca
     2461 tcccggtagg tttcggggat gtcgacgatc ttgtaggccg tggacggcgg tgccttgatg
     2521 ccctggggag cagacggcgc agacattttg gcagtcgatg cggacttctg tgccgcagcc
     2581 ttccgggcag cggacgtggc cttactcgca gtagtagcca tgttcatccc ctcctaattg
     2641 cgcaccggcg caggcaccgg cattgctcct tagcgtccaa actgggctcg tagcgacacg
     2701 taccacatca acggcgcgtt gtagtaacag ccaacctcgc ccgctcccgc gttcggcgag
     2761 cctgccgtgc agtagcggtc attggtcagg ttggtgccgc cgaaggcgaa gtcgtacttg
     2821 gcaccgggtg agatatagtg aatcgatgca tccagctgcc tcgaggccgg ccggcgcagc
     2881 tccggggtgt tcaacgcgtc attccagatc tccccggtgt aagtgaacgc cgcgagcagg
     2941 cgcagcgtcg actggctcgg gagtatgtag tcgtaactcg gccagatcga gcccttccac
     3001 ttcggagtct tcggcagcct cgcatccagc gccgtcacgc ccggtggctg aaacgcgca
//
LOCUS       DATDMP010000339         4610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_165068, whole genome shotgun sequence.
ACCESSION   DATDMP010000339 DATDMP010000000
VERSION     DATDMP010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165068"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /gene="shc"
                     /locus_tag="VI195_05930"
     CDS             <1..1499
                     /gene="shc"
                     /locus_tag="VI195_05930"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY6823959.1"
                     /translation="WRGVPYVPVEIVLLPRWFPFNLHKVSYWSRTVIVPLTILCTLKP
                     RARNPRNVRLRELFTTPPEQERHYFRRPGGRPTALARIFLAIDRLARSVDRLIPARLR
                     ERALERAESWMLQRLNGEDGLGAIFPAMVNALEALAARGYRSDDPRRLAAKLALQRLL
                     IVNNSSAYCQPCISPVWDTALASLAMQEVGCPDALAAASRGLQWLQPRQLLEAPGDWR
                     TKRPTLAGGGWAFQRANSYYPDLDDTAVVAWAMHRSAERERYVPAVDLALDWLVGMQS
                     SNGGFAAFDVDNTSYYLNEIPFADHGALLDPPTSDVTARVVTVLALVGRPHDAAALER
                     ANAYLRAEQEADGSWFGRWGTNYLYGTWSVLTALAQAGIGPDDAAVRRAVSWLEARQN
                     ADGGWGESNDSYARECIDGRAASTPHQSAWAVLALLAAGGVGSESVRRGIHYLLRTQS
                     ADGLWSDPNFTAPGFPRVFYLRYHGYCAYFPLWALAAYRARARLGPFH"
     gene            1514..2233
                     /locus_tag="VI195_05935"
     CDS             1514..2233
                     /locus_tag="VI195_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823960.1"
                     /translation="MAALVAEARTLGPAMRHGDALASLADGTLLVVSGMGPVAARQGA
                     RRLIEAGAGALMSWGMAGGLDPALATGTLLLPSEVVSPEGGVFPTARAWRERLCTALA
                     ASRPVCVGRLLTCREPIGTAAGKASAFHRTAAAAVDMESFAVAEVAARGGLPFIAVRV
                     IVDAAGDALPRALLAAAAGTGAVHAGRLLGGLARAPADLPALIRLVRKFRAARRTLSI
                     AARSGALGPPGIPCPCRSGSS"
     gene            2230..3219
                     /gene="hpnA"
                     /locus_tag="VI195_05940"
     CDS             2230..3219
                     /gene="hpnA"
                     /locus_tag="VI195_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEY6823961.1"
                     /translation="MRALVTGATGFIGSAVARALCRAGWQVRVLARAGSDRRNLLQLP
                     VEIATGDLTDCPSLDQALRDCAAVFHAAADYRLGVADAETLYRTNVEGTRNVLNAAQR
                     AGAARIVYTSSVATMGLPADGSPGTEDTPVTLADMIGHYKRSKFLAEELVRDFAKAGV
                     PVIIVNPSTPIGPGDVKPTPTGRVVVDAALGRMPAYVDTGLNIVHVDDVAAGHLQALD
                     RGRVGERYILGGENLSLREILAHISGLARRKPPRIRLPHTALLPLALASETIARITGG
                     SARVTLEGVRMARKHMYFSSDKAVRELGYRWRPSAAAFEDAVAWFREQGVLRP"
     gene            3426..>4610
                     /locus_tag="VI195_05945"
     CDS             3426..>4610
                     /locus_tag="VI195_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY6823962.1"
                     /translation="MERAFELQHSAVKRRWAVVLPERLRILLAALLVGALAAMNFLVG
                     LGQPSHPIWDETYYLTAAQRYEDGTAQFAAHPPLGLMLIAAGDALLHPNRGTDTRAIG
                     WDKQVAGGRLPEHYSFAGMRLMSGVFAVIGAVAFFAVMLALTRAVPAALLLSNLYVFE
                     NAFIAHFRAAHLDAFQLAFALAALLCFVVSVRRGQRSSPWLEFGLGAACGLAIMVKLN
                     AVLWTLLGLMLILRRIGKDWHSAPRARLLLTGLRDGTVMASGCLVAIVAVWTIHVAVT
                     PHLPPPGSPAGARDEAFMSHTYRAYLEGTTEFSPMVVLAAAQDYARFMLADLKGVPKN
                     DQNGSQPVEWLLHIGTINYRWDADGAHTSYVQLVGNLWGWLMADICLVATVGLLILQW
                     WRP"
BASE COUNT          686 a         1493 c         1574 g          857 t
ORIGIN      
        1 cctggcgtgg cgtgccttat gtgccggtcg agatcgtgct gctgccgcgc tggtttcctt
       61 tcaatcttca caaagtctcc tattggtcac ggacggtgat agtgcccctc accatcctgt
      121 gtaccctgaa gccccgcgcc cgcaatccgc gcaacgtgcg cctgcgcgag ttgttcacta
      181 cgcctcccga gcaggaacgc cactactttc gccgtcccgg cggacgcccc acggcgctgg
      241 cgcggatctt cctcgcgatc gatcggctcg cccgcagcgt cgatcggttg atccccgcga
      301 ggctgcgcga gcgcgcgctc gagcgagcgg agtcttggat gctacagcgg ctgaacggcg
      361 aggatggtct gggcgccatc tttcccgcca tggtgaacgc gctcgaggca ctcgcggctc
      421 gcggctaccg cagcgatgat ccgcgccggc tcgctgcgaa actggccttg cagcgactct
      481 taatcgtcaa caactcgagc gcttactgcc agccatgcat ctcgccggtg tgggatacgg
      541 cgctggcaag tctcgcgatg caggaggtgg gctgtcccga cgcgctcgca gcagcaagtc
      601 gcggactgca gtggctgcag ccgcgacagc tcctcgaagc cccgggggac tggcgtacga
      661 aacgtcctac gctcgcgggc ggcggctggg cattccagcg cgccaacagt tactatcccg
      721 atctcgacga cacggcggtg gtcgcctggg ccatgcaccg ctcggcagag cgcgaacgct
      781 acgtgcccgc cgtagacctc gctctggatt ggttggtcgg catgcagagc agcaacggcg
      841 gctttgccgc gttcgacgtc gacaacacca gctattacct caacgagatt ccattcgccg
      901 atcacggcgc attgctggat cctccgacga gcgacgtcac ggcccgtgtc gtgacggttc
      961 tagcgctggt gggccgtccg cacgatgccg cggcactcga gcgcgccaat gcctacctgc
     1021 gcgccgaaca ggaggcggac ggctcatggt tcggccgctg gggcaccaac tacctctacg
     1081 gcacgtggtc ggtgttgacc gccctggctc aggccggcat aggccctgat gacgcagcgg
     1141 tgcggcgtgc ggtgagctgg ctcgaggcgc ggcagaatgc ggacggcggc tggggcgaga
     1201 gcaatgatag ctatgctcgc gagtgcatcg atgggcgcgc ggcgagcaca cctcaccaaa
     1261 gcgcctgggc ggtactcgct ctgctggcgg cgggcggagt cggatcggag agtgtgcggc
     1321 gcggaattca ctacctgttg cgtacgcaga gtgcggatgg gctgtggagc gacccgaact
     1381 tcacggctcc cggattcccg cgggtgtttt atctgcgtta tcacggctac tgtgcctact
     1441 tcccgctgtg ggcgctggcc gcctatcgcg cgcgcgcacg gctcggaccc tttcattgaa
     1501 tggactggga attgtggcgg cgctcgttgc cgaggcgcgc acgctcggcc cggcgatgcg
     1561 gcacggtgat gcgctcgcct cccttgccga cggcacgctg ctggtcgtca gcgggatggg
     1621 acccgtcgca gccaggcagg gcgcgcgccg gctgatagaa gccggggcgg gggcgctcat
     1681 gagctggggc atggccggcg ggctcgatcc ggccctggcg accggaaccc tgttgctgcc
     1741 gagcgaagtc gtttcaccgg aaggcggggt attccccacg gcgcgtgctt ggcgcgagcg
     1801 gctctgcacc gcgctggcag cctcgcgacc ggtctgcgtg ggcagactgc tgacctgccg
     1861 cgagccgatc ggcacggccg ccggcaaagc aagcgccttt caccggacgg cggcggccgc
     1921 agtggacatg gagagtttcg ccgtcgcgga ggttgcggcc cgaggcggtc tgccttttat
     1981 cgccgtgcga gtgatcgtcg acgccgctgg ggatgccctg ccccgggcgc tgcttgccgc
     2041 tgccgcaggc acgggggcgg tgcacgccgg gcgactgctc ggcgggctcg cccgcgcgcc
     2101 ggcggatctt cccgcgttga ttcgcctggt gcgcaagttc cgggccgccc gccggacgct
     2161 gtcgatcgct gcgcgcagcg gcgctcttgg accgcccggg attccctgcc cttgccggtc
     2221 gggcagctca tgagggcgct ggtcacagga gcgaccggct tcattggctc agcggttgcc
     2281 cgagcgcttt gcagggcggg ttggcaagtc cgcgttctgg cgcgcgccgg ctccgaccgg
     2341 cgcaatctcc tgcagctgcc ggtcgagatc gcgaccggcg acctcaccga ctgtccctcc
     2401 ctcgatcagg cgctgcgcga ttgcgcggcg gtgtttcatg ccgccgccga ctatcgtctc
     2461 ggggtagcgg acgcggagac gctgtatcgg accaatgtgg aggggacccg caatgtgctc
     2521 aacgctgcgc agcgcgccgg agcggcgcgc attgtctaca cgagcagcgt ggccaccatg
     2581 gggttaccgg ccgacggctc gcccggcacg gaggacacgc cggtcacgct tgctgacatg
     2641 atcgggcact acaagcgttc gaaattcctc gcggaagagc tggtgcgcga tttcgccaag
     2701 gcgggcgtgc ccgtaatcat cgtcaacccg tctactccga taggaccggg cgatgtcaag
     2761 cccacgccga cgggacgggt ggtggtggac gcggcgctcg gacggatgcc ggcgtacgtc
     2821 gacaccggac tcaacatcgt gcacgtggac gatgtcgccg ccggtcatct gcaggccctg
     2881 gaccgcgggc gcgtcggtga gcgctatatt ctgggcggcg agaacctgag cctgcgggag
     2941 attttggcgc acatcagcgg cttggcacgc cgcaagccac cgcgcattcg cctgccacac
     3001 accgcgctcc tgccgctcgc gctggcatcc gaaaccatag cgcggataac cggcggtagc
     3061 gcgcgagtga cgctcgaggg cgtgcgcatg gcacgcaagc acatgtattt ctccagcgat
     3121 aaggccgttc gggaattggg atatcggtgg cgaccgtccg ccgccgcatt cgaggatgcg
     3181 gttgcctggt ttcgcgagca gggcgtcctg cggccctgac gccgcacgtc aaacccaaac
     3241 atcccgcgat tttcctcgcg tttctcgcgc tgatgctcac ttggtgccag ctcatgccac
     3301 cgccccagga aaggggcgtc ggcggggcaa catctgctgg tgcgcgacct tatcggtgcg
     3361 ggcgctgatt accgcgttct ggatcacgga tccccgggac tggcaggggc agactgccgg
     3421 ttcacatgga acgcgctttc gagctacagc actccgcggt caaacggcga tgggccgtgg
     3481 tcctgcctga acggctccgc atcctgctcg ccgccctgct ggtcggcgcg ctggccgcga
     3541 tgaatttcct ggtcggactc ggccagccct ctcacccgat atgggatgag acgtactacc
     3601 tgaccgcggc gcagcgctac gaggacggaa ccgcacaatt cgccgcccat ccccccctgg
     3661 ggttgatgct gatcgccgcc ggcgacgcgc tccttcatcc caatcgcgga actgacacgc
     3721 gcgcaatcgg ctgggacaag caggttgcag gcgggcgtct gccggagcac tactcattcg
     3781 cgggcatgcg gctgatgtcc ggagtgtttg cggtgatcgg agcggtagct ttttttgccg
     3841 taatgttggc tttgacgcgt gccgtgcccg ctgcgctgtt gctcagtaat ctatacgtgt
     3901 ttgaaaatgc gttcatcgcg cactttcgag ccgcccatct cgatgcgttc caacttgcgt
     3961 tcgcgctggc tgcgctgctg tgtttcgttg taagtgtgcg ccgtggccag cgcagctcgc
     4021 cttggctgga gtttggtctg ggcgcagcct gcgggctggc cattatggtc aagctgaatg
     4081 cggtgctgtg gaccctgctt ggcctgatgc tgattctccg ccgtatcgga aaggactggc
     4141 attccgctcc gcgagcacgc ctgctcctga cgggtctgcg cgatggcacg gtgatggcgt
     4201 cgggctgcct ggtggccatc gtggccgtgt ggacgattca cgttgcggtc acgcctcatc
     4261 ttccgccccc gggcagtccc gcgggtgcaa gagatgaagc cttcatgtcg catacctacc
     4321 gggcatacct cgagggcaca acagagttct ctccgatggt ggtgttggcc gcggcgcagg
     4381 actacgcacg cttcatgttg gctgacctaa agggtgtgcc gaagaacgat cagaatgggt
     4441 cgcaaccggt ggaatggctg ctgcacatcg gcaccatcaa ttaccggtgg gacgcggatg
     4501 gtgctcatac gtcgtacgtg caactggtcg gaaacctctg gggatggctg atggcagaca
     4561 tttgcctcgt tgccacggtc gggttgctga tcttgcagtg gtggcgtccg
//
LOCUS       DATDMP010000340         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1831262, whole genome shotgun sequence.
ACCESSION   DATDMP010000340 DATDMP010000000
VERSION     DATDMP010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831262"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VI195_05950"
     CDS             complement(<1..522)
                     /locus_tag="VI195_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY6823963.1"
                     /translation="MRAILLAAGQGLRLQYPPDRQLPKCLLRIGGTSLLERHRQLLTS
                     GGVEEIVLAVGFRRDLIEAELDRLDWRPQLIVNERYDRGSMLTLHTVADPLTRGGDVL
                     LMDADVLYHERIMALLVEGAQPLNRLLIDRKFEDGDEPVKVCIRSGKPIELRKRLAPG
                     LQHDTIGESVGFFR"
     gene            complement(602..1591)
                     /locus_tag="VI195_05955"
     CDS             complement(602..1591)
                     /locus_tag="VI195_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY6823964.1"
                     /translation="MKRSTAWLLSAGVLLFVSEIGLHSLPAVYATLAIAGWGLLLVTL
                     FHLLPLAVDAAAIRVLLPARSSWRDTVLARWAGESASSLLPFGQLAGPVVMMRQLWRR
                     GVLMQNAVAAVTVSTTLQVLAQIAFALLGLALIAGQLSRHAENALRPALLVASSVLAV
                     LAITFYALQRRGLFTWSMRAISRFCGKRDWSSLHGRAREIDEAVHTTYRHGRRAPASF
                     LLNLAGWIAGTGEVYLLLQLFGTPVTWGVALMLESVGQAIRGAAFAIPGALGVQEGGY
                     LLLAPLAGLHPDTALALSLAKRAREVLLGVPGLLHLHASESLWRPRPPASTGE"
     gene            complement(1588..2334)
                     /locus_tag="VI195_05960"
     CDS             complement(1588..2334)
                     /locus_tag="VI195_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823965.1"
                     /translation="MAARLRELDIAPLQRTFAQQGAFLYLPEFLPAAITARLVAAVQA
                     VGSAVNRNYLPRHKQGGSVSRHVLDRCAPLVAELYGSPALIGWLECLAGERLQLSPPK
                     DPHAYALYYYTQPGDHIGWHYDTSYYAGRRYTVLLGVIDESSCRLEYQLHTRAKATHT
                     VSGSVQIPPGGLVFFNGDAVRHRITPLGAGEKRIALTFEYVTDPNMRAWRRFVSNMKD
                     AVAYFGFRQVFRHRVRTVPERRPDADSGRT"
     gene            complement(2418..3098)
                     /locus_tag="VI195_05965"
     CDS             complement(2418..3098)
                     /locus_tag="VI195_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY6823966.1"
                     /translation="MSELPQVSPKLARPSQWDARLARRLVLPLKDSPVTPNDLTTLRL
                     ATGLAAALAFIPGSYGWSNIAALLLVVSNFIDHADGELARISDKASRLGHLYDLASDT
                     LVTVLLFVAMGVGVRAKGGLPLDIPPVVLGAIAGSAIALIFFLRMRIEEMAGKAASRQ
                     RAFGGFETEDVLYLLPLATLCDGVAPLLLAASVGAPLFAVRVVLDYQRLLRRPKSAAQ
                     STSDAGLA"
     gene            3296..>3997
                     /locus_tag="VI195_05970"
     CDS             3296..>3997
                     /locus_tag="VI195_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6823967.1"
                     /translation="MGRLLPLLALLVGSYAARAADAPELVVTNARIHTQDAAHRVVTA
                     LAVRGNAILATGSDTDMRALAGPRTRMVDLGGRIVLPGLIDAHIHPAQSAQDLDKCNL
                     HDRELDAAGVKAAVAACMKARPPARQEWFEVAQVDASDLVLSRAELDGMRSDGPLVLE
                     SADGHSGWGNSAALAAAHIDRNSRDPLGGRIERDPQGEPTGALRDAAEEKLLAAIPAP
                     AVAVRVERLERAFALM"
BASE COUNT          739 a         1317 c         1321 g          620 t
ORIGIN      
        1 ccgaaagaag ccgaccgact cgccgatggt gtcgtgttgc aacccgggag caagccgttt
       61 gcgcagctcg atcggtttgc cgctccgtat gcacaccttt accggctcat ctccatcctc
      121 gaacttccgg tcgatgagca accggttgag cggctgcgct ccctcgacga gcagcgccat
      181 gatgcgctcg tgatacagga catcagcgtc catgagcaaa acgtcgcccc cccgcgtcaa
      241 ggggtcggcg accgtatgaa gcgtaagcat gctgccgcga tcgtagcgct cgttgacgat
      301 gagctgcggt cgccaatcga ggcggtcgag ttcggcctcg atcagatcgc ggcggaagcc
      361 aacggcgaga acgatctcct cgaccccgcc gctcgtgagc agttgtcggt gcctctcgag
      421 caggctcgtt ccgccgattc gcaacaggca cttgggcagt tggcggtccg gggggtattg
      481 cagacgcaga ccttggccgg cggcgagcag gatggcgcgc aaatcactct ccttaatcta
      541 agcaggcagc acaaatatca cgaggctagc cgccagagta cggtcgcggg tgcggatcgg
      601 cttattcgcc cgttgaagcg ggtggtcgag gacgccaaag gctctccgat gcgtgaagat
      661 gcaacaaacc gggcacgccg agcaggactt cgcgtgcccg cttggcgagc gacagggcca
      721 gtgccgtgtc ggggtgcagc cctgcgaggg gcgcgagcag cagataccca ccttcctgca
      781 ctcccagtgc cccggggatg gcgaaggccg cgccgcgaat tgcctgtccc acactctcga
      841 gcatcagggc cacgccccag gtgaccggcg tgccaaacag ctgcagcagc agatacacct
      901 ctccggtacc cgcgatccag ccggcgagat tcagcagaaa gctggccggc gcccgcctgc
      961 catgccgata ggtcgtatgc acggcttcgt cgatttctcg cgctcgaccg tggagcgagg
     1021 accagtcacg ttttccgcag aagcgggaga tggcgcgcat cgaccacgtg aataagcccc
     1081 gccgctgcag ggcatagaac gtgatcgcca gtacggcgag cacgctactg gccaccagca
     1141 aggcggggcg taacgcattc tctgcatgcc ggctcagttg gccggcgatc agcgcgagtc
     1201 cgagcagggc aaaggctatt tgcgcgagca cctgtagtgt tgtgctgacg gtgactgcgg
     1261 cgacggcatt ttgcatcaaa acaccgcggc gccaaagctg ccgcatcatc acgaccgggc
     1321 ctgccagctg gccgaaaggc agcaggctgc ttgcggactc tcccgcccag cgggccagca
     1381 cggtatcgcg ccaggagctg cgcgccggca ggagtacccg gatcgctgcc gcatcgaccg
     1441 cgagcggcag caggtgaaac aaggtgacca gcagcaatcc ccaaccggca atcgcgagcg
     1501 tggcgtacac cgcgggaagg ctgtgcaagc cgatctcgct gacaaacagc agcacgccgg
     1561 ccgagagcag ccaggccgtc gaccgtttca tgtacgtcca gaatccgcgt ccgggcgacg
     1621 ctccggcacc gtccgcactc gatggcggaa gacttgccgg aacccgaagt aggcgaccgc
     1681 gtccttcatg ttcgagacga agcgtcgcca ggcgcgcatg ttcgggtcgg tcacgtactc
     1741 gaaggtcagc gcgatgcgtt tttcgcccgc gcccaacggg gtgatgcgat ggcgcaccgc
     1801 atcgccattg aaaaagacca agccaccggg tggaatctgc acggaccccg acaccgtgtg
     1861 cgtcgccttc gcgcgcgtgt gcagctggta ctcgagccgg cacgaggatt catcgatgac
     1921 tccgagcagc actgtgtagc gccggcccgc atagtaagag gtgtcgtaat gccagccgat
     1981 gtggtctccc ggctgcgtgt agtaataaag agcgtatgcg tgcggatctt tcgggggtga
     2041 cagctgcagg cgctcccccg ctaggcattc cagccagcca atgagcgccg gcgagccata
     2101 gagctccgcc accaagggcg cgcaccggtc caacacatga cggctgacgc tgccgccctg
     2161 cttgtgccgt ggcagataat tgcggttgac ggcgctcccg actgcttgca cggcggccac
     2221 cagccgcgca gtgatcgcgg cgggcaaaaa ctcaggcagg taaagaaacg ccccctgctg
     2281 cgcgaacgtt cgctgcagcg gcgctatgtc cagctcgcgc aggcgcgcag ccaccgtccg
     2341 atcggtgtcc gactgagggt gcattcccgc cgcacaggca gccaagctcg cctgctgtga
     2401 atcgcgggcc gcggccgtca ggccagcccc gcatcggaag tcgactgagc cgccgatttc
     2461 ggccggcgca gcaagcgctg gtagtcgagc accacccgca cggcgaacag cggggccccg
     2521 accgaggcgg ctagcagcag cggagcgacg ccatcgcata gggtcgccaa gggtagcagg
     2581 tacagtacgt cctccgtctc gaatccgccg aatgcacgtt gccggctcgc cgctttgccg
     2641 gccatttcct cgatgcgcat acgcagaaaa aagatcaacg cgatcgcgct accggcaatt
     2701 gcgcccaaga cgaccggcgg aatgtccagt ggcaggccgc ccttggcgcg gacgccgacg
     2761 cccatcgcga caaaaagcag caccgtgacc agcgtgtcgg atgccaggtc atagagatgg
     2821 ccaaggcggc tggccttgtc actgatgcgg gccagttcgc cgtcggcgtg atcgatgaaa
     2881 ttcgacacca ccagcaatag cgccgcgatg ttggaccagc catagctgcc cggaatgaat
     2941 gccagcgcgg cagccagccc cgttgccagg cgcagcgtgg tcaaatcatt gggcgtgacc
     3001 gggctgtcct tgagcggcag gacgaggcgg cgcgcgagtc tcgcgtccca ctgtgatggc
     3061 ctagcgagtt tcggcgagac ctgtggtagc tcggacatga cgctccatgc aaagggccgc
     3121 tcggcgcgat cgccttgccg ctatgttacc ctacgatatt cggcgaatgc gcgccggcgc
     3181 caggtttcgt agatgcattc gatgcgagca ccccggaaga ggtgagcacc tgagcgcgcc
     3241 ggcagcatcg atcaccctgg gctcggcgga ctcgccacgg ctgaatccgg cggccgtggg
     3301 gaggctgctc ccgttactcg cgctgctcgt gggttcgtat gccgctcggg ccgccgacgc
     3361 gccggaactc gtggtcacga acgcgcgcat tcacacgcag gacgcggcgc atcgtgtcgt
     3421 gaccgccctg gcggtgcgcg gcaatgccat cctggccacg ggctccgaca ccgatatgcg
     3481 cgcactggca gggccgagga cccgcatggt cgatctcggc gggcgcatag tgctgccggg
     3541 tctcatcgac gcgcacatac acccggcgca gagtgcgcag gacctcgaca agtgcaacct
     3601 ccacgaccgc gagctggatg ctgccggagt gaaggctgcg gtagccgcat gcatgaaggc
     3661 gcgtccgccg gccaggcagg agtggttcga ggtcgcgcag gtcgacgcct cggacctggt
     3721 gctctcgcgt gccgagctcg atggcatgcg ttcggatggc ccgctggtgc tcgagagcgc
     3781 cgacggccac agcggctggg gcaacagcgc ggcgctcgcc gccgcacaca tcgaccgcaa
     3841 cagtcgcgac ccgcttggcg gccgcatcga acgcgatcca cagggcgagc cgaccggtgc
     3901 gttgcgcgat gcggccgagg agaaactgtt ggccgctatc ccggctccag cggtcgccgt
     3961 gcgggtggag cgccttgaac gcgcctttgc cctcatg
//
LOCUS       DATDMP010000341         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_942857, whole genome shotgun sequence.
ACCESSION   DATDMP010000341 DATDMP010000000
VERSION     DATDMP010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942857"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /gene="ppx"
                     /locus_tag="VI195_05975"
     CDS             complement(<1..1487)
                     /gene="ppx"
                     /locus_tag="VI195_05975"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY6823968.1"
                     /translation="MARHDLLAAVDLGSNSFHLQVGRVSGGQIYPLDSLREVVRLGAG
                     LTSEKRIDRATQAQALEALGRIGERLRGLPRSAVRAVGTNALRVAKNAPQFLREARSA
                     LGFPIEVIAGREEARLIYLGVAHSTPPSAANRLVVDIGGGSTEFIIGKGLEPLLTESL
                     YMGCVSYSLEYFEGGKVEKSRMKKAELAARQELAGMVRGYRELGWETAVGSSGTARAI
                     EAILRENGFSEEGITRDGLDRLRSLLIKHERADPDRIAGLRKDRAPVLPGGVAIMGAA
                     FDELSIERMAVSDGALRHGVLYDLLGRTQHRDMREATIAEFVRRYRVDAAQAERVRLL
                     ALRIFDSINREKEDQETDRLLLDWAARVAEIGLSVAHAQYQKHSAYILSNADMPGFSR
                     MEQQRLARVVLAHRGKLGKMQDAGLEGGDWTLVFALRIAALVLRNRTDARFPMLRVEG
                     DKSGYSIDLPEAWLEENPLAAAALDTERKHWEAVDLRFSVGSLSE"
     gene            1607..>2049
                     /locus_tag="VI195_05980"
     CDS             1607..>2049
                     /locus_tag="VI195_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY6823969.1"
                     /translation="MVKKNLPASHFLNRELGLLEFNRRVLAQAADDSVPLLERLRFLT
                     IVSSNLDEYFEIRVAGLQEQIKAGIKESGPDGRSPAEVYELVSAQAHALVGEQYRLLN
                     NVLLPALAKQGVIFPRREDWTEPQRAWIRDYFMRELLPVLTPIGL"
BASE COUNT          305 a          720 c          683 g          341 t
ORIGIN      
        1 ttctccgaca ggctgccgac cgagaagcgc aggtcgaccg cttcccagtg cttgcgctcg
       61 gtgtcgagcg cggccgcggc cagcgggttt tcctcgagcc aggcttccgg caggtcgatg
      121 ctgtagcccg acttgtcgcc ctcgacgcgc agcatcggga agcgcgcgtc ggtgcggttg
      181 cgcagcacga gcgcggcgat gcgcagcgcg aacaccagcg tccagtcgcc gccctccagc
      241 cccgcgtcct gcatcttgcc gagcttgccg cggtgcgcga gcacgacgcg cgcgaggcgc
      301 tgctgctcca tgcgcgagaa acccggcatg tcggcgttgg acaggatgta ggccgagtgc
      361 ttctggtact gcgcgtgcgc caccgacaag ccgatctccg cgacgcgcgc cgcccagtcg
      421 agcagcaggc ggtccgtctc ttgatcctct ttttccctgt tgatggaatc gaaaatcctc
      481 agggccagaa gccgcaccct ttcggcctgc gccgcgtcca cgcggtagcg gcgcacgaat
      541 tcggcgatcg tggcctcgcg catgtcgcgg tgctgcgtgc ggccgaggag atcgtagagc
      601 acgccgtggc gaagcgcacc gtcggacacc gccatccgct cgatgctcag ctcgtcgaac
      661 gccgcgccca tgatggcgac gccgcccggc agcaccggcg cgcgatcctt gcgcagcccg
      721 gcaatgcggt ccgggtccgc gcgctcgtgt ttgatgagca ggctgcgcag ccggtctagg
      781 ccgtcgcgcg tgatgccctc ctcggagaag ccgttctcgc gcaggatggc ttcgatcgcg
      841 cgcgccgtgc ccgaggagcc caccgccgtc tcccagccca gctcgcggta gccgcgcacc
      901 atgccggcaa gctcctggcg cgccgcgagc tcggccttct tcatgcgcga cttctccact
      961 ttgccgccct cgaaatactc caggctgtag ctcacgcagc ccatgtagag gctctcggtg
     1021 agcagcggct cgaggccctt gccgatgatg aactcggtag agccgccgcc gatgtcaacc
     1081 accaggcggt tcgccgctga gggcggcgtc gaatgcgcga cgccgaggta gatgaggcgc
     1141 gcttcctcgc ggccggcgat cacttctatg ggaaaaccca gggcgctcct ggcctcgcgc
     1201 aggaactgcg gcgcgttctt cgccacgcgc agcgcgttgg tgccgaccgc gcgcactgcg
     1261 ctcctgggca gcccgcgcag ccgctcgccg atgcgcccga gggcttccag cgcctgcgcc
     1321 tgggtggcgc gatcgatgcg cttctcggaa gtgaggccgg cgcccaggcg cacgacctcg
     1381 cgcagcgagt ccagcggata gatctggccg ccgctgacgc gcccgacctg caggtggaaa
     1441 ctgttcgatc ccaggtcgac ggcggcgagc agatcgtgac gggccatggc agtcgctgag
     1501 cttacaccct gtggataagt cggcaagtga cggttttgcg gcgtttcatg gagctgcaat
     1561 atttgtcctg taagctgttc ggaggagaag gtccccgcgt ttcccgatgg tcaagaagaa
     1621 cctgcccgcg tcgcatttcc tcaatcgaga gctcggcctg ctcgagttca accggcgcgt
     1681 gctcgcgcag gccgccgacg attcggtgcc gctgctcgag cggctgcgct tcctcaccat
     1741 cgtctcctcg aacctggacg agtacttcga gatccgcgtg gccggcctgc aggagcagat
     1801 caaggcgggc atcaaggaat ccggccccga cggccgcagc ccggccgagg tctacgagct
     1861 ggtctcggcg caggcgcacg cgctggtggg cgagcagtac cgcctgctca acaacgtgct
     1921 gctgcccgcg ctcgccaagc agggcgtcat cttccccagg cgcgaggact ggaccgagcc
     1981 gcagcgcgcc tggatccgcg actatttcat gcgcgagctg ttgccggtgc tcacgccgat
     2041 cggcctcga
//
LOCUS       DATDMP010000342         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_942867, whole genome shotgun sequence.
ACCESSION   DATDMP010000342 DATDMP010000000
VERSION     DATDMP010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942867"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VI195_05985"
     CDS             <1..890
                     /locus_tag="VI195_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY6823970.1"
                     /translation="RSAAGASATGAVRGVVFDVDGTLLLSNRSLGGYEVLPGAIELLS
                     ALKERRVPFALLTNGSAYPPAEQAAKLRGVGLPVDDEQMLTPSSIAADLMRRRGIRRA
                     LVLGMHGVGHALRAAGIETVYTGEPGATEVQCVFVGWHPECGMKDIETACQAIWGGAR
                     LYVASDVPFFATKQGRTIGYSFAITAAIRRLTRAPMTLTGKPAVRALRFAARRLGVPV
                     ESVAVVGDDPLVEILMAHRAGATALAVTTGTTAREEWLRQTGTRRPHDILNDLREVLN
                     WVAEVGEPAARGGIRRVVK"
     gene            905..1270
                     /gene="ligA"
                     /locus_tag="VI195_05990"
     CDS             905..1270
                     /gene="ligA"
                     /locus_tag="VI195_05990"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: protein motif:HMM:NF009917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HEY6823971.1"
                     /translation="MPLSKPYADIPGTTIFDTDQARRGYHLNMFCMSLMKPENRARFK
                     ADERSYLDTWPMTEEQKLAVLDRDYNRMISLGGNIYFLAKLFATDGKSFQYVAAQMAG
                     MSQAEYAKMMLEGGRAPQG"
     gene            1296..>2066
                     /locus_tag="VI195_05995"
     CDS             1296..>2066
                     /locus_tag="VI195_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY6823972.1"
                     /translation="MRAALYYGRQDLRLGSVPEPEPGPGEVKLRVHYNGICGSDLLEY
                     YDGPVTTRSAPHPLTGVQNPVIMGHELCGEVVRLGDGVGDLGIGDLVAVEPVETCGQC
                     LYCGLGQYNHCPSLAIQGYNRAGGLADYTVVKRLRAHKLPRGVSALQGALIEPLAIAW
                     HTVNRCAVSAGQMVAIHGAGPIGAGAFLTLRRRGVEAIVVDPSPARRAALAALGARHL
                     LDPSACDVVAAIRDLTGGRGAHASIDAAGVPQAFAAMLH"
BASE COUNT          328 a          670 c          735 g          333 t
ORIGIN      
        1 tgcgcagtgc ggcgggcgcg agcgccaccg gcgcggtgcg cggcgtggtg ttcgatgtcg
       61 atggcacgct gctgctcagc aatcgctcgc tcgggggtta cgaggtgttg ccgggcgcga
      121 tcgagttgct cagcgcgctc aaggagcggc gggtgccttt cgcactcctc accaacggca
      181 gcgcctatcc gccggcagag caggccgcga agctgcgcgg cgtcgggctg ccggtcgatg
      241 acgagcagat gttgactcct tcgagcatcg ccgcggatct catgcgtcgc cgcggcatcc
      301 ggcgggcgct ggtgctcggg atgcatggcg tcgggcatgc actgcgcgcc gccggcatcg
      361 agacggtgta taccggtgag cccggcgcga ccgaggtcca atgcgttttc gtcggatggc
      421 atccggagtg cggcatgaag gacatcgaga ccgcatgcca ggcaatctgg ggcggcgcac
      481 gactctatgt cgcctcggat gtgccgttct tcgcgaccaa gcagggccgc acgatcggct
      541 actcgttcgc catcaccgct gccatccgcc ggctgacgcg cgcacccatg acactgaccg
      601 gaaagccggc ggtgcgcgcg ttgcgcttcg ctgcgcgcag gctcggcgtg ccggtcgagt
      661 ccgtggcggt ggtgggcgat gacccgctgg tcgagatcct catggcgcac cgcgccggcg
      721 ccacggccct ggcagtgacc acggggacga cagcgcgcga ggagtggctc cggcaaaccg
      781 gcacgcgccg accgcacgat atattgaatg acctgcggga ggtgctgaat tgggtcgccg
      841 aagtcggcga gcccgctgcg cgcggtggga tccgccgcgt ggtgaagtga gaggaagccc
      901 tgcgatgcca ctcagcaaac cctacgccga tattcccgga acgacgatct tcgacaccga
      961 ccaggcgcga cgcggctatc acctcaacat gttctgcatg tcgctgatga agccggagaa
     1021 tcgtgcgcgc ttcaaggccg acgagcgcag ctacctggat acctggccca tgaccgagga
     1081 gcagaagctt gcggtgctcg accgcgacta caaccgcatg atctcactcg gcggcaacat
     1141 ctactttctt gccaagctgt ttgcgaccga cggcaagagc ttccagtacg tcgccgcaca
     1201 gatggccggt atgtcgcagg cggagtacgc gaagatgatg ctggagggcg ggcgcgcgcc
     1261 gcagggctga gacgcgcggg cggagacgat cagcgatgcg cgcagcgctc tattacggtc
     1321 gacaggatct gcggctcggc tccgtgccgg agccagagcc cgggcccggc gaggtcaagc
     1381 tgcgcgtgca ctacaacggc atctgcggca gcgacctgct cgagtactac gacggacccg
     1441 taaccacgcg cagcgcccca cacccgctta ccggagtaca gaacccggtg atcatggggc
     1501 acgagctgtg tggagaagtc gtgaggttgg gtgatggcgt cggcgatctc ggcatcgggg
     1561 atctggtcgc cgtcgagccg gtggagacct gcgggcagtg cctgtactgc ggtctcggtc
     1621 agtacaacca ttgtccctcg ctcgctattc agggttacaa ccgcgcgggc ggcctggccg
     1681 actacacggt ggtcaagcgg ctgcgtgcgc acaagcttcc gcgcggcgtg agcgcgctgc
     1741 agggcgcgct gatcgagccg ctcgccatcg cgtggcacac ggtgaatcgt tgcgcggtga
     1801 gcgcaggcca gatggtcgcc atccacggcg ccgggccgat cggtgccggt gccttcctga
     1861 cgctgcgccg ccgcggcgtc gaggcgatcg tcgtggatcc atccccagcg cggcgcgcgg
     1921 cgctggccgc actcggtgcc cggcatctgc tcgatccgag cgcttgcgat gtggtcgcgg
     1981 cgatccgtga ccttaccggt ggccgcggtg cgcacgcgtc gatagacgcc gcaggagttc
     2041 cgcaggcctt cgcggcgatg ctgcac
//
LOCUS       DATDMP010000343         5498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_831941, whole genome shotgun sequence.
ACCESSION   DATDMP010000343 DATDMP010000000
VERSION     DATDMP010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831941"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VI195_06000"
     CDS             complement(<1..553)
                     /locus_tag="VI195_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY6823973.1"
                     /translation="MSTADLRSSFLEFFRGQSHIIVPSSSLVPANDPTLLFTNAGMVQ
                     FKEVFLGKEQRDYLRAASAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIHLAWDFLIYTLRLDPARLWCTVYRDDDEAADVWLKDIGISPERFARLGESSNFW
                     AMGDTGPCGPCSEIFFDHGPAIPG"
     gene            complement(568..999)
                     /locus_tag="VI195_06005"
     CDS             complement(568..999)
                     /locus_tag="VI195_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY6823974.1"
                     /translation="MALLARRDFLCRELTERLTRKGFAESAAASAVAGLVHEGVLDEA
                     RCAQNYVAYHAGRGQGPIRIAAELRRRGLGNELIEAALAGGPDWSALARSVSRAKFGP
                     QPPASWAQRARQARFLQYRGFSADHIRAATGADVDTGFTDL"
     gene            1142..1600
                     /locus_tag="VI195_06010"
     CDS             1142..1600
                     /locus_tag="VI195_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823975.1"
                     /translation="MRIVCNRSIAAMLTLLGAGLLPVALADPPADPGVTAPAAATAAA
                     AAPAATSTSSTAATAASSNAAASSTAAHTAAASTAETDAALEKRLTNSGYKPRMRNGT
                     KVWCKQQGELGSRLGGQEVCATPDEWRRAVRENQDVVEHIQRIDSHASGK"
     gene            complement(1640..2671)
                     /gene="recA"
                     /locus_tag="VI195_06015"
     CDS             complement(1640..2671)
                     /gene="recA"
                     /locus_tag="VI195_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY6823976.1"
                     /translation="MEENRKKALAAALGQIEKQFGKGSVMRLGDAAASYDIETVSTGS
                     LGLDIALGVGGLPRGRVVEVFGPEASGKTTLTLQVIAEVQRSGGTAAFVDAEHALDPT
                     YAEKLGVNINDLLVSQPDTGEQALEITDMLVRSNAVDIVVVDSVAALTPKAEIEGEMG
                     EMQLGLQARLMSQALRKLTGNIKRSNTIVVFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRIGAIKSGEEVTGNMTRVKVVKNKVAPPFREAEFEIMYGQGISREGEIIE
                     LASSLGIIEKSGAWYAYKGSRIGQGKDNARNYLQTNKEIAREIEEQVRARLLPPRQQR
                     NGDQAVNSA"
     gene            complement(2777..3346)
                     /gene="thpR"
                     /locus_tag="VI195_06020"
     CDS             complement(2777..3346)
                     /gene="thpR"
                     /locus_tag="VI195_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY6823977.1"
                     /translation="MRERAGATRRLFFALWPDEAQRGELEHAAAKAVRRCGGRPVPPA
                     NLHVTLAFLGSVAIARIPELQRIARERTVSLAMHAPISLSFAQLVHWKEAQILCALSA
                     PESTDARALAVALQEAAVAIGLSPDRKPFRAHVTLARKVLHSGTLPPLRPVLWRFAAF
                     ALIDSRTEPSGPVYSVIDSYPLVDDNPRD"
     gene            complement(3346..3885)
                     /locus_tag="VI195_06025"
     CDS             complement(3346..3885)
                     /locus_tag="VI195_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY6823978.1"
                     /translation="MSETELYELAARVGHKLRVAGRRIVTAESCTGGWVSKALTDVPG
                     SSQWFECGFITYSNAAKVRDLGVSAGTLELFGAVSEQTVREMAEGALRVSHANVALAV
                     TGIAGPDGGAPGKPVGTVWFCAAAREDSAIDIIAEQQFFDGDRAQVRSGSVQHALRLI
                     LRLDLPVRPEAGSRTQAPV"
     gene            4100..4933
                     /locus_tag="VI195_06030"
     CDS             4100..4933
                     /locus_tag="VI195_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823979.1"
                     /translation="METVTVEGRRERKDLEREVNQFVFAVAVRYLHDSLSRWNTPVCP
                     LVAGLPHEQGEFVLGRLSQIARSAKAPLAGEHCRGNFYVIVTPEPEALIRQWQRHDPR
                     TFNNRDGMGYLRNFRDVPRAVRVWYNTGFVSSEGKPILSSDELAGALVGSSLGMTLQM
                     AQVPINKISTGSRVTYSAVRPLSSVIVIVDAKRVQTLSFGQLADYIGMVGLAEVNQDA
                     EVGTAPTILRLFRSTDALPPRLSTWDEAFLASLYGVDQGSVVEQPMMKRQMVSSLAPV
                     H"
     gene            5005..>5498
                     /locus_tag="VI195_06035"
     CDS             5005..>5498
                     /locus_tag="VI195_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823980.1"
                     /translation="MTASILHRAMINPLSVAAALAVSGCWTAPVADVQPKGEPRLIQG
                     GIAVESVKDPAIVHSVDPGARTLVLSTRPGVASTYKAGAQVENLGRIKAGDTVRATVA
                     EELTVYVLRDGQLPGPGGMHQTIASDARVLWVDPSYRLLTLRYPDGRDETFKAPLGVK
                     LGEM"
BASE COUNT          936 a         1845 c         1772 g          945 t
ORIGIN      
        1 cgcccggaat ggcgggtccg tgatcgaaaa agatctcgct gcaggggcca cacgggccgg
       61 tatcgcccat ggcccagaag tttgagctct cgccgagacg cgcgaagcgt tcggggctga
      121 tgccgatgtc cttcagccag acatccgccg cttcgtcatc gtcgcggtac accgtgcacc
      181 acagccgcgc cgggtcgagc ctgagggtgt agatgaggaa gtcccacgcc aggtgaatcg
      241 cctcccgctt gaaatagtct ccgaaggaga aatttcccag catttcgaaa aaggtgtggt
      301 ggcgcgccgt gtagccgacg ttctcgagat cgttgtgctt gccgcccgct cgcacgcagc
      361 gctgcgcgga ggcggcgcgc aggtagtcgc gctgctcctt gccgagaaac acctccttga
      421 actgcaccat gcccgcgttg gtgaacaaga gcgtcggatc gttcgccggt accagcgagc
      481 tcgagggcac gatgatgtga ctctgcccgc ggaaaaattc caggaaagaa ctgcgcaagt
      541 ccgcagtact catgtacgaa ggcttgttca caggtccgta aatcctgtat cgacgtcagc
      601 accggtagcc gcgcggatat gatccgctga aaagccacga tactgcaaaa agcgtgcttg
      661 tcgtgcccgc tgcgcccagc tggccggcgg ctgcggtccg aatttcgccc gcgagacgct
      721 gcgggcgagt gcgctccagt ccgggccgcc cgcgagcgct gcctcgatga gctcgttccc
      781 gaggccgcgc cggcgcagct cggccgcgat gcgtatcgga ccctgtcccc ggcccgcgtg
      841 gtaggcgaca tagttctgcg cgcagcgggc ctcgtccagc accccctcgt ggaccagccc
      901 ggccacggca ctcgctgctg cagactcggc gaagcccttt cgcgtgaggc gttccgtcaa
      961 ctcgcggcag agaaaatcac gtcgcgcgag gagcgccatg gcggccatgc gcgcggcgtg
     1021 ttcatcccgc ggcgctgtcg cagcggcagc ccttgggaat ctgcgtgaag gcatcgatta
     1081 cagtatagtg agcgcacagc agcccggatt gagcgggtgc tgcttctagc gaggaaggct
     1141 catgcgcatc gtttgcaacc gatcgatcgc cgcgatgttg acgctgctcg gcgccggcct
     1201 gctcccggtg gctctcgccg atccccccgc ggacccgggc gtcacggccc ccgcggcagc
     1261 caccgcagcc gccgctgcgc cggccgcgac cagtaccagc agcacggcgg ccaccgccgc
     1321 cagttcgaat gccgctgcct catcgacggc ggcccacact gcagcagcgt ctaccgcgga
     1381 aaccgatgcg gccctggaaa agcggctgac gaactctggc tacaagccac gaatgcgcaa
     1441 cggaacgaag gtctggtgca agcaacaagg agagctcggc tctcgcctcg gaggtcagga
     1501 agtctgcgcc actcccgatg agtggagacg ggccgtgcgc gaaaaccagg acgtcgtcga
     1561 gcacatacag aggatcgaca gccacgcctc tggcaaatga gccggcagct ctgctgacgc
     1621 cgacggaaat ggtcggtgtt caggcgctgt tgaccgcctg atccccgttg cgctgctgcc
     1681 gcggcggcag cagccgcgcc cggacctgct cttcgatctc gcgcgcgatt tccttgttcg
     1741 tctgcagata gttgcgggcg ttgtccttgc cctggccgat gcggctgccc ttgtacgcgt
     1801 accaggcgcc ggatttctcg atgatgccga gcgagctggc gagctcgatg atctcgccct
     1861 cgcgcgagat accctggccg tacatgatct cgaattccgc ctcgcggaag ggcggagcga
     1921 ccttgttctt cacgaccttt acccgcgtca tgttgccggt gacttcctcg ccgctcttga
     1981 tggcgccgat gcggcggatg tcgagccgca cggaagcata gaacttcagc gcgttgccgc
     2041 cggtggtggt ctcgggattt ccgaacatca ccccgatctt catgcggatc tggttgataa
     2101 acaccacgat ggtattcgag cgcttgatgt tgccggtgag cttgcgcagc gcctgcgaca
     2161 tgagccgcgc ctgcagcccc agctgcatct cacccatctc gccttcgatc tcggccttcg
     2221 gcgtgagcgc cgccaccgaa tcgaccacca cgatgtcgac cgcattggag cgcaccagca
     2281 tgtcggtgat ctccagcgcc tgctcaccgg tatccggctg cgaaaccagc agatcgttga
     2341 tgttcacgcc gagcttctcg gcataggtgg gatcgagcgc atgctcggcg tcgacgaatg
     2401 cggcggtgcc accgctgcgc tgcacctcgg cgatcacctg cagcgtgagg gtggtcttgc
     2461 ccgaagcctc cggtccgaag acctccacca cacggccgcg cggcagacca ccgacgccga
     2521 gcgcgatgtc gagcccgagc gaacccgtgg acaccgtttc gatgtcgtag cttgccgccg
     2581 cgtcgcccag gcgcatcacc gagcccttgc cgaactgctt ctcgatctgt cccagagcgg
     2641 cggcgagcgc cttcttgcgg ttttcttcca tctgcgaatt tcccgaaggt cttggtgaag
     2701 cgagaggaaa cattgcgctg ccgattatca cacaaagcat cgcgagcggg gcgcatcacg
     2761 ccgccgcgtt tctcacctaa tcgcgtggat tgtcgtcaac cagtggatag gaatcgatga
     2821 cactgtatac cggcccgctc ggctcggtgc ggctgtcgat gagagcgaaa gcagcgaagc
     2881 gccacagcac cgggcgcaac ggcggcagcg tcccggaatg caggaccttg cgcgccagag
     2941 tgacatgcgc ccggaagggt ttgcgatcgg ggctgaggcc aatggccacc gcggcctctt
     3001 gaagcgcgac ggccagggcg cgggcatccg tcgattcagg cgcgctaagc gcgcacagga
     3061 tctgtgcctc cttccagtgc accagctgcg cgaagctgag cgagatcgga gcatgcatcg
     3121 cgagcgacac ggtgcgctcg cgtgcgatcc gttgcagctc gggaatgcgc gcgatcgcca
     3181 cgctcccgag gaacgcgagc gtcacatgca gattcgcagg cggcaccggg cggcccccgc
     3241 accgccgcac cgccttggcg gccgcgtgct cgagctcgcc acgctgcgcc tcatccggcc
     3301 agagtgcgaa gaacagccgg cgggtggccc ctgcccgctc gcgcatcaca ccggagcttg
     3361 ggtgcgcgag ccggcttccg gtctcaccgg cagatccagc cgcaggatga gccgcagcgc
     3421 gtgctgcacc gagccgctac gcacctgcgc gcgatccccg tcgaaaaact gctgctcggc
     3481 aatgatgtcg atggcgctgt cctcacgcgc cgccgcgcag aaccataccg tgccgacggg
     3541 cttgcccggc gcgccgccat cgggcccggc gatgcccgtc accgcaagcg cgacgttggc
     3601 gtgcgagact ctgagcgccc cctcggccat ctcgcgcacc gtctgctcgc tcactgcacc
     3661 gaagagctcg agcgtcccgg ccgacacccc gagatcgcgc accttggcgg cgttcgagta
     3721 cgtgatgaag ccgcattcga accactggga gctgcccggc acatcggtga gcgctttgga
     3781 gacccagccc ccggtgcagg actccgcggt gacgatgcgc cggccggcca ctcgcagctt
     3841 gtgcccgacg cgcgccgcga gctcatacag ctctgtctcg ctcaccgtga caggctaccc
     3901 caccggcaac gtctcctggt agccgctcag cgcccctttt ttggggtagc cttcgcggca
     3961 aatggcgaga atcgcagtac ccctcttgtg cattgcgggc gccctgatgt cgctgccgcc
     4021 tggctcgcat gccaaagcgc tcgccggaca atcccagtcg tctgcggccg caactgcttc
     4081 gtcctctggc aaggccccgg tggagacggt gacggtggaa gggaggcgcg agcgcaaaga
     4141 cctcgagcgc gaggtaaacc agttcgtgtt cgcggtggcg gtgcgctacc tgcacgattc
     4201 gctttcgcgc tggaacacgc cggtgtgtcc attggtggca gggctcccgc acgagcaggg
     4261 cgagttcgtg ctggggcgcc tgtcgcagat cgccaggtcg gcaaaggcac cgctcgccgg
     4321 tgagcactgc agaggcaact tctacgtcat cgtgacgccg gagcccgagg ccttgatcag
     4381 gcagtggcag cggcacgatc cgcgcacctt caataaccgc gacggcatgg gatatttgcg
     4441 gaattttcgc gatgtccccc gcgcggtgcg cgtctggtac aacacgggct tcgtgtcctc
     4501 cgagggcaag ccgatcctct cctccgatga gctggccgga gcgctggtgg gcagtagtct
     4561 cggtatgact ctgcagatgg cgcaggtgcc gatcaacaaa atcagcacgg gctcgcgggt
     4621 tacatactct gctgtgcgac cgctgtcctc ggtcatcgtg atcgtcgacg ccaagcgcgt
     4681 gcagacgctc agtttcggtc agctggccga ttacatcggc atggtcgggc tcgccgaggt
     4741 caaccaggat gcggaggtcg gcacggcgcc cactattctg cggctgtttc gctccaccga
     4801 cgctctgccg ccacgcctga gcacctggga cgaagctttt ctcgcctcgc tctacggggt
     4861 tgatcagggg agcgtcgtgg aacaacccat gatgaagagg cagatggtga gcagcctcgc
     4921 acctgtgcac tgacgcgcac cgtgcgctcg ccaggcactt tcctttagac cgcttgcgtc
     4981 atcacctatc catcggcact gaacatgacc gcctcaatcc tgcaccgagc aatgatcaat
     5041 ccgctgtcgg tggccgcggc gctggcggtc tcgggttgtt ggacggcacc ggtggccgat
     5101 gtacagccaa agggcgagcc gcgcctgatt cagggcggca tcgcagtcga gtcggtcaag
     5161 gacccggcaa tagtgcactc ggtggacccc ggcgcgcgca ccctggtgct gagcacgcgg
     5221 ccgggcgtgg cctccaccta caaggccggc gcgcaggtgg agaacctcgg tcggatcaag
     5281 gcgggcgaca cggtgcgggc cacggtcgcg gaagagctca ccgtttatgt attgcgagac
     5341 ggccagctgc ctgggcccgg tggcatgcac cagaccatcg catccgacgc ccgggtgttg
     5401 tgggtcgatc cgagctatcg cctgctgacg ttgcggtatc cggacggccg cgacgagacc
     5461 ttcaaggccc ccctgggagt caagctcggc gagatgga
//
LOCUS       DATDMP010000344         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_165849, whole genome shotgun sequence.
ACCESSION   DATDMP010000344 DATDMP010000000
VERSION     DATDMP010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165849"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VI195_06040"
     CDS             <1..186
                     /locus_tag="VI195_06040"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY6823981.1"
                     /translation="VGTSSDSWEKAAAAAVTQASKSLRDLRIVEVVELDMQVQNGKVR
                     AYRARVKLSFKTESGKK"
     gene            complement(390..1091)
                     /locus_tag="VI195_06045"
     CDS             complement(390..1091)
                     /locus_tag="VI195_06045"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEY6823982.1"
                     /translation="MPQHIGIVACSAEGAALGYRTVCLEGAQLLGPFDHPEVSLHNHP
                     LAAYMRCIDRDDWPGVAELMLSSAHKLARAGADFLICPDNTIHQALPWVLRRSPLPWL
                     HIAEVVAAEATARGFRTLGITGTRYLVDSEVYPEKLAAQGLGFQRPNAAERAEINRII
                     FEELVCGVLRPEAAAYLQGVFARMRAEGCDAVILGCTELPLIMDDANSPLPTLDSTRL
                     LSRAALRRATQRTGS"
     gene            1228..>2537
                     /gene="pgtP"
                     /locus_tag="VI195_06050"
     CDS             1228..>2537
                     /gene="pgtP"
                     /locus_tag="VI195_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter protein PgtP"
                     /protein_id="HEY6823983.1"
                     /translation="MIGLLRPAPFAPPLPAAQVDQVYRRLRRRVFAGIYIGYAGYYLV
                     RNNLALAIPDILREHPQYTKAALGSALTGLSIAYGLSKFLMGSVSDRSNPKYFLPLGL
                     LLSSALMAAFGLVRAAGASLLLIALIQALNGWVQGMGWPPCGKSMVHWFSTRERGLLV
                     SVWNTAHNIGGALVANLALAGMLLFHDWRAKFYFNALIAAALAIGVHFLLQDTPQSRG
                     LPPVEQYRNDYPPGYSAAHERTLGFREILFGSVLSNRYLWAIAIANAFCYFVRYGVVN
                     WIPTYLETAKGFSFRQSSLGWSLYEYAAIPGTIACGWLSDRVFRGKRAPATMLFMALT
                     LVAVVVYWLNMHGPLWLDYAALIAIGFLVYGPIMIIGLHSLDLVPKKAAGTAAGFTGF
                     FGYVFGSATAGTGVGWIADRFGWGGVFTAMVACCLLTIFFSALTL"
BASE COUNT          365 a          875 c          888 g          409 t
ORIGIN      
        1 gtcggtacca gcagcgattc gtgggagaag gcggccgccg ccgcggtgac ccaggcgtcg
       61 aagagtctgc gggacctgcg catcgtcgag gtggtcgagc tcgacatgca ggtgcagaac
      121 ggcaaggtcc gcgcctaccg cgcccgcgtc aagctgtcat tcaagaccga gagcggcaag
      181 aagtagccgg ccgaacgcgg caggcggcgg ggtcgcccgg cgacagttaa gcggttgctt
      241 aactatcgtg ccgcggacgc cgcacgccac acgccacggg ccgcacgcca cgggccacgc
      301 accacgcacc acgcaccacg cgccacgcac cacgcgccac gcgccacgcg ccacgcgccc
      361 cgggtggcgc ggcgcgcggt gctcgtccct caggaccccg tgcgctgcgt cgcgcggcgc
      421 agcgccgcgc gcgagagcag ccgggtggaa tcgagcgtcg gcagcggcga attggcgtcg
      481 tccatgatca gcgggagctc ggtgcagccg aggatcaccg cgtcgcagcc ctcggcgcgc
      541 atgcgcgcaa acacgccctg caggtaggcg gcggcttcgg gccggagcac cccgcacacc
      601 agctcctcga aaatgatgcg attgatctcg gcgcgctcgg cagcgttcgg tcgctggaac
      661 ccgaggcctt gcgcagcgag cttttcgggg tagacctcgc tgtcgaccag gtaacgcgtc
      721 ccggtgatgc cgagagtgcg aaagccccgg gcggtcgcct cggccgccac tacctcggcg
      781 atgtgcagcc acggcagcgg cgagcgccgc agcacccagg gcagcgcctg gtgaatggta
      841 ttgtccgggc agatgaggaa gtccgcgccc gcccgggcga gcttgtgcgc cgaggacagc
      901 atgagctcgg cgaccccggg ccagtcgtcg cggtcgatgc agcgcatgta ggcggccagc
      961 gggtggttgt ggaggctgac ctccgggtga tcgaagggtc cgaggagctg tgcgccctcg
     1021 aggcacaccg tgcggtagcc gagtgccgcg ccttcggcgg agcaggcgac gatcccgatg
     1081 tgctggggca tgcgtcgatc ttaccgggcg ggccgcggca gcgggaggcg gcgcccattg
     1141 ctcaatcgag ccggcgcggg gcgataatga gccccgaatg agcgtcgagc gtgtgcgcct
     1201 tggcttgcgg cccgccgggg cgcgccggtg atcggactcc tcaggccggc accgttcgcc
     1261 ccgccgctgc ctgccgccca ggtggatcag gtgtaccggc ggctgcgccg cagggtattc
     1321 gccggcatct acatcggcta cgccggctac tacctggtgc gcaacaacct cgcgctcgcg
     1381 attcccgaca tcctgcgcga gcatccgcaa tacaccaaag ccgcgctcgg cagcgcgctc
     1441 accggattgt cgatcgccta cggactgtcc aagttcctga tgggctcggt gtccgaccgc
     1501 agcaacccga agtacttcct gccgctgggg ctgctgctct cgagcgccct gatggcggcg
     1561 ttcggcctgg tgcgggcggc cggcgcatcg ctgctgctga tcgccctcat ccaggcgctc
     1621 aacggctggg tgcagggcat gggctggccg ccgtgcggca agagcatggt gcactggttc
     1681 agcacccgcg agcgcgggct gctggtctcg gtgtggaaca cggcgcacaa catcggcggg
     1741 gccctggtcg cgaacctggc gctcgccggg atgctgctgt ttcacgactg gcgggcgaag
     1801 ttctatttca acgcgctgat cgccgcggcg ctggcaatcg gggtgcattt cctgctgcag
     1861 gacacgccgc agtcgcgcgg cctgccgccg gtcgagcaat atcgcaacga ctatccgccc
     1921 ggctactctg cggcgcatga gcgcacgctc ggtttccgcg agatcctgtt cggcagcgtg
     1981 ctgtcgaacc gctacctgtg ggcgatcgcg atcgccaacg cgttctgcta cttcgtgcgc
     2041 tacggcgtcg tgaactggat tccgacctac ctcgagaccg ccaagggctt ctcgttccgg
     2101 cagtcgagcc tcggctggtc gctgtacgag tacgcggcca ttccgggaac gattgcctgc
     2161 gggtggctgt cggaccgggt gttccgcggc aagcgcgccc ccgccaccat gctgttcatg
     2221 gcgctcacgc tcgtcgccgt ggtcgtctac tggctcaaca tgcacggccc gctgtggctc
     2281 gactacgcgg cgctcatcgc catcggcttc ctcgtctacg gcccgatcat gatcatcggc
     2341 ctgcattcgc tcgatctggt gccgaagaag gccgcgggca ccgccgccgg cttcaccggt
     2401 ttcttcggct acgtgttcgg ttcggcgacc gcgggcaccg gggtgggctg gatcgccgac
     2461 cgcttcgggt ggggcggggt gttcacggcc atggtcgcgt gctgcctgct cacgatcttc
     2521 ttcagcgccc tcacgct
//
LOCUS       DATDMP010000345         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2054420, whole genome shotgun sequence.
ACCESSION   DATDMP010000345 DATDMP010000000
VERSION     DATDMP010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054420"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VI195_06055"
     CDS             <1..608
                     /locus_tag="VI195_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY6823984.1"
                     /translation="PLEHKRHLLRHLIERGSWRQVLVFTRTKHGANRLTQQLQAAGIP
                     AAAIHGNKSQAARTRALSDFKEQRIAALIATEVAARGLDIRELPHVVNFELPFVPEDY
                     VHRIGRTARAGRSGVAISLVAPDEDPLLRDIERLLGEKLPTASLPQYPVPAGAAPLSS
                     HRNAGRPPPRAPHRGARARGRAQHERAPRGPQATARPHGRR"
     gene            complement(615..1535)
                     /locus_tag="VI195_06060"
     CDS             complement(615..1535)
                     /locus_tag="VI195_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823985.1"
                     /translation="MSGAYAAARSARAALLASLLLLVACPAGAEELQVGGALLELDFE
                     RGAFGSGAPQILEWVRRSADIVARYYGRFPAARVTVRLVPMDDGGVHGGKTFANPRAY
                     IRVQLGREVTAQQLLGDWVLVHEMTHLALPDTGETHAWLSEGLATYVEGIARVQAGNR
                     SEADVWAEEMRSMPRGLPAPGDRGLDHTHTWGRTYWGGAMFCLLADVEIRRRTALRFG
                     LQQALRAILRESGGLATDWPIERVLRTGDAAVGTKTLEDLYAQMKDTPVTPDLMTLWR
                     ELGVVPEGNSVRLSDDAPLAAVRRAIMSAP"
     gene            complement(1543..>2072)
                     /locus_tag="VI195_06065"
     CDS             complement(1543..>2072)
                     /locus_tag="VI195_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY6823986.1"
                     /translation="MPAILDHDAPCGPTSVFESGAILLYLAQKSGKLLPRDVRGQVEV
                     TQWLFWQMAGLGPMAGQNHHFARYAPEKIPYAIERYVKETNRLYGVLNHRLNDRRYLA
                     GDYSIADIACYPWIVPYEAQGQRLEQFPHLRRWFESIRERPATVRAYARAASVNTQDT
                     VTEQSRRVLFGQSAR"
BASE COUNT          331 a          741 c          679 g          321 t
ORIGIN      
        1 tgccgctgga gcataagcgc catctgctgc gacatctcat cgagcgcggc agctggcggc
       61 aggtattggt gttcacgcga accaagcacg gcgccaaccg tctcacgcag cagttgcagg
      121 ccgccggcat ccccgccgcg gcgatccacg gcaacaagag ccaggccgca cgcacccgcg
      181 cgctgagcga tttcaaggag cagcgcattg cggcactcat cgccacggag gtggccgcgc
      241 gtgggctcga cattcgcgag ttgccccacg tggtcaactt cgagctgcct ttcgtgcccg
      301 aggactacgt gcatcgcatc ggccgcacgg cgcgcgcggg tcgttccggg gtcgccatct
      361 cgctggtggc gccggatgag gacccgctgc tgcgcgacat cgagcgcttg ctgggagaga
      421 agttgcctac cgcctcgctg cctcagtacc cggtgccagc gggcgcagcg ccgctttcct
      481 cgcaccgcaa tgcgggccgc ccgccgccgc gcgcgccgca ccgcggagcg cgcgctcgcg
      541 gcagggcgca acacgagcgc gctccgcgtg ggccgcaagc cactgcccgc ccgcacggcc
      601 ggcgctagcg cgcctcaggg cgcgctcatg atggcgcgcc gcacggctgc cagcggcgca
      661 tcgtcgctca acctcacgga gtttccctcg ggcacgacgc cgagctcgcg ccacagcgtc
      721 atgagatcgg gtgtcaccgg cgtgtccttc atctgcgcgt agagatcctc gagcgtcttc
      781 gtgccgactg cggcatcgcc ggtgcgcagc acgcgctcga tcggccagtc ggtggcgagc
      841 ccgccgctct cgcgcagaat ggcgcgcagt gcctgctgca ggccgaagcg cagcgccgtg
      901 cgccggcgga tctcgacgtc ggcgagcagg cagaacatgg cgccccccca gtaggtgcgc
      961 ccccaggtgt gcgtgtgatc gagaccgcga tcaccaggcg cgggcagccc gcgcggcatg
     1021 gagcgcattt cctccgccca cacgtcggct tcactgcggt tgcccgcctg tacgcgcgcg
     1081 atgccttcca cgtaagtcgc caggccctcc gacagccagg catgcgtctc gccggtgtca
     1141 ggaagtgcaa ggtgcgtcat ctcgtgcacc agcacccagt cgccgagcag ttgctgtgcc
     1201 gtgacctcgc gtccgagctg cacgcggata taggcgcgag ggttagcgaa agtcttaccg
     1261 ccatgcaccc cgccatcgtc catcggcacg agccggaccg tgacacgcgc cgccggaaag
     1321 cgcccgtaat agcgcgcgac gatgtccgcc gaccgtcgca cccactccag aatctgcggt
     1381 gcgccgctgc cgaatgcgcc gcgctcgaag tcgagctcca gcagcgcgcc accgacctgc
     1441 aactcctcgg cgcctgccgg gcacgccacg agcagcagga gcgatgccag cagcgccgcc
     1501 cgcgcactgc gcgcagccgc gtacgcgccg ctcaccgtgc gctcagcgcg cgctctgtcc
     1561 gaagagaacg cgcctcgatt gctcggtgac ggtgtcctgg gtgttgacac ttgcagccct
     1621 ggcataggcg cgcaccgttg ccggacgctc acgaatgctc tcgaaccagc gtcggagatg
     1681 cgggaattgc tcgaggcgct gaccctgcgc ctcgtacggc acgatccagg gatagcaggc
     1741 gatatcggcg atcgagtagt ccccggcaag gtagcggcga tcgttgagac ggtgattgag
     1801 cacaccgtag aggcggttgg tctcttttac gtaacgctcg atggcgtacg gaatcttctc
     1861 cggcgcgtag cgcgcgaagt gatggttctg tccggccatc ggacctaagc ccgccatctg
     1921 ccagaacagc cattgcgtca cctcgacctg accgcgcacg tctcgcggca gtagcttgcc
     1981 gcttttctgt gcgaggtaaa gaagaatcgc gcccgattcg aagacgctcg ttgggccgca
     2041 cggtgcatca tgatccagga tcgccggcat ct
//
LOCUS       DATDMP010000346         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1165353, whole genome shotgun sequence.
ACCESSION   DATDMP010000346 DATDMP010000000
VERSION     DATDMP010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165353"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VI195_06070"
     CDS             complement(<1..359)
                     /locus_tag="VI195_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823987.1"
                     /translation="MTLPFAEPPRSVCILRLSALGDACHVVPIVRTLQQAWPATQLTW
                     IIGRNEVRLMKLLEGVEFITVDKRATLGALAALRTALAGRRFEVLLHMQLALRASLVA
                     ACVAARVKLGFDRARAR"
     gene            372..1136
                     /locus_tag="VI195_06075"
     CDS             372..1136
                     /locus_tag="VI195_06075"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEY6823988.1"
                     /translation="MNGRFALGPEVKRSAITGGAMLYDTSRVGNLSADWFEPRYWESL
                     GALDGSARGRAAARFVTNSDGRSYVLRHYRRGGLMARLSSDRFAWRGEESTRPFAEWQ
                     LTYRLHRAGLPVPAPVAARYSHRGSSYTGDIITERLPVVGSLAECLRTGALATLTWIA
                     IGRCIRRFHDLGVCHADLNAHNVLLSEESIYLIDFDRCQLRAPGLWRDGNLVRLRRSL
                     EKVTWALPAERFGESDWHALLDGYRQSAARSEAPPT"
     gene            1157..2446
                     /gene="waaA"
                     /locus_tag="VI195_06080"
     CDS             1157..2446
                     /gene="waaA"
                     /locus_tag="VI195_06080"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEY6823989.1"
                     /translation="MRFLYLLAIYLAVPVVSAVYLWRGLRDRSYWRHFPERFGFGARL
                     VPQGVWLHAVSVGEVQACAPLVSALNRRHPHLPLTVTTFTPTGSARARALFGNLAEVR
                     YVPYDLPGAVRRFFRRVQPRLAVIFETELWPNLYRECSRRRIPLVLASARISARSVSR
                     YRRLGALFRETLARTALVAAQGAGDAERFRALGADPATTHVTGNIKFDFELPEGLAAR
                     GARLRAQYAPDRPLWVAGSTHGGGEEQALLEAQRQVRATHPGALLVLAPRHPPRFAEV
                     AQALETSGLAFVRRSEAASGASDASCAVLLLDSLGELLDFYAAADLAFVGGSLVPIGG
                     HNLLEPAALGVPILTGPNNSNSEEIARLLIARGAAEVVHDAAELGARVTALLADGAAR
                     ERMGAAGRASVDSNRGALVKLLALIEPLLEQPEELGR"
BASE COUNT          346 a          881 c          871 g          392 t
ORIGIN      
        1 tcgcgggcgc gggcgcgatc gaagccgagc ttcacgcgcg ccgccacgca ggctgccacc
       61 aggctggcgc gcagcgcgag ctgcatgtgc agcagcacct cgaagcggcg gccggcgagc
      121 gcggtgcgca gcgcggcgag cgcgccgagc gtggcgcgct tgtcgacggt gataaactcc
      181 acgccctcga ggagcttcat gagacgcacc tcgttcctgc cgatgatcca ggtcagctgc
      241 gtggccggcc atgcctgttg taaggtgcgc acgatcggca cgacgtggca ggcatcgccg
      301 agggccgaga gccggaggat gcagacactg cgcggcggct cggcgaaagg caacgtcatg
      361 gagtcagctt tgtgaatggg cgcttcgcgc tcggaccgga ggtgaaacgc agcgccatca
      421 cggggggcgc catgctatat gacacctccc gtgtgggcaa tttgagtgcc gactggttcg
      481 agccgcgcta ctgggagtcg ctcggagcgc tcgatggcag cgcgcgcggc cgcgcggcag
      541 cccgcttcgt caccaactcc gatggacgga gctacgtgct gcgtcactac cgccgcggcg
      601 gcctgatggc gcgcctgtcc tcggatcgct tcgcctggcg cggggaggag agtacgcgcc
      661 ctttcgccga atggcagctc acctatcgac tgcaccgtgc cggtctaccg gtgcccgcgc
      721 cggtggcggc acgctacagc catagaggtt cgagttacac cggcgacatc atcaccgagc
      781 ggctaccggt cgtcggctcg ctggccgagt gcctgcgcac cggcgccctc gcaaccctca
      841 cctggatcgc catcggccgc tgcatccgcc gcttccacga cctcggcgtc tgccacgccg
      901 atctgaatgc gcacaatgtg ctgctcagcg aggagagcat ctacctgatc gatttcgatc
      961 gctgtcaact gcgcgccccg ggcctgtggc gcgacggcaa cctcgtgcgg ctgcgccgct
     1021 cgctcgagaa ggtcacctgg gcattgcccg ccgagcgctt cggcgaatcc gactggcacg
     1081 cgcttctcga tggctaccgc cagtcagccg cgcggagcga ggcgccgccg acctgaggca
     1141 cggggcggac gccgctttgc gatttctgta cctgctcgcc atctatctgg ccgtcccagt
     1201 ggtctccgcc gtttacctgt ggcgcggtct gcgcgatcgc agctactggc gccatttccc
     1261 cgagcgcttc ggcttcggcg cgcgcctcgt gccacaaggc gtgtggctgc acgccgtctc
     1321 ggtgggcgaa gtgcaggcct gcgccccgct cgtgagcgcc ctcaaccgcc gccatccgca
     1381 tctgccactg acggtaacca ctttcactcc caccggctcg gcccgggcgc gggcgctgtt
     1441 cggcaatctg gccgaggtgc gctatgtgcc atacgacctg cccggtgcgg tgcgacgctt
     1501 cttccgccgt gtgcagccac ggctggcggt gatcttcgag accgagctct ggccaaatct
     1561 ctatcgcgag tgctcccggc gtcgcatacc gctcgtgctg gcgagcgcac gcatctcggc
     1621 acgctcggtg agccgttacc ggcgcctcgg cgcgttgttc cgcgagacgc tggcgcgcac
     1681 cgcgctggtc gccgcacagg gggccggcga tgccgagcgt ttccgtgcgc tcggagcgga
     1741 ccctgcaacc acccacgtga cgggcaacat caagttcgac ttcgagctgc cggagggact
     1801 cgcggcgcgc ggcgcacggc tgcgcgcgca gtacgcaccg gaccggccgc tgtgggtcgc
     1861 aggcagcacg cacggcggcg gcgaggagca ggcgttgctc gaggcacaac ggcaagtgcg
     1921 cgccacgcac ccgggcgcgc tgctcgtgct cgcaccgcgc cacccgccgc gcttcgctga
     1981 ggtggcgcag gcgctcgaga cgagcggcct cgcgttcgtg cgccgctccg aagcggcaag
     2041 cggtgctagc gacgcgagct gcgcggtgct gctgctcgat tcactcggtg agctgctgga
     2101 tttctacgcg gccgcggatc tcgccttcgt cggcggcagt ctcgtcccca tcggcggcca
     2161 caacctgctc gagcccgcgg cgctcggtgt gccgattctg actggtccga acaactccaa
     2221 cagcgaggag atcgcacggc tgctgatcgc gcgcggtgcc gcggaagtgg tgcacgatgc
     2281 ggcggagctc ggcgcgcgcg tcactgcctt gctcgcagac ggcgcagcgc gcgagcgcat
     2341 gggcgcggcc gggcgcgcct cggtggacag caaccgcggc gcgctcgtga agctactcgc
     2401 cctgatcgag ccgctgctgg agcaacccga ggaactcggg cgctaacgcc tgccctgcgg
     2461 gcgcggcggc ggcggagtct tcgggggcgg
//
LOCUS       DATDMP010000347         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1388920, whole genome shotgun sequence.
ACCESSION   DATDMP010000347 DATDMP010000000
VERSION     DATDMP010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388920"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1926
                     /gene="glmS"
                     /locus_tag="VI195_06085"
     CDS             91..1926
                     /gene="glmS"
                     /locus_tag="VI195_06085"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY6823990.1"
                     /translation="MCGIVGAVAERNVVPILMEGLRRLEYRGYDSAGIAVLNGSKHLT
                     RLRTVGKVRMLDEALEQTPTDGKIGIAHTRWATHGVPSERNAHPHISHDGLAIVHNGI
                     IENHEELRADLLRRGYQFASETDTEVIAHRVHLHLQTLPDLFKAVRATVAELEGAYAL
                     VVVSEHDPQRLILARQGCPVVIGLGVDENFVASDVAALLPVTRRFIFLEEGDVAEVRR
                     TAVTIIDRDGALVTRPVRESELSANAAERGPYQHFMLKEIHEQPRAIADTLQERVANG
                     RLLEAAFGPAASGVLARTRNVHIVACGTSYHAACVARYFIEQISKVPCAVDIASEYRY
                     RKPLVPPNSLFVTISQSGETADTLAALRLARHSGYLSSLAICNVPESSLVRESELVML
                     TRAGPEIGVASTKAFTTQLTALGMLVVALARYQGSDAERERALVRGLIELPAMVERAL
                     ELDPVVHRLAERFADKRHALFLGRGALYPIALEGALKLKEISYIHAEGYPAGELKHGP
                     LALVDADMPVVAVAPNNDLLEKLKSNLMEVRARGGELFVFADPEARISASDGVTVIEM
                     PRQVTYVQSPVVYSIPLQLLAYHAAILKGTDVDQPRNLAKSVTVE"
     gene            1994..>3128
                     /locus_tag="VI195_06090"
     CDS             1994..>3128
                     /locus_tag="VI195_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY6823991.1"
                     /translation="MTRTTVPDILAGLAIAGLLLPEAVAYSGLAGLPPQAGVIALFAG
                     LICYGAIGQSRAAIVSATSSSAAVLASALVALGASSVAERVALASILVASSGVWFALC
                     AVLRLGAMSNLIARPVLRGYTFGLALVIAVRQWPHLANVPTHSSGFFALILELLRSYR
                     AWQLPSLACGLGALAGLFLLERVPRVPGALVVIVASILAAPLLAAHAVALTGPIELHL
                     TLPALSVPPGIHTLALLEYSLALMFILYAESYGSIRTFALKHDEPVQPNRDLLALGVA
                     NLLSGLLRATPVGAGYSATSANDAAGARSRLAGLSAAAVVLLLVLAFLPWIERIPQPV
                     LAAIVIHAVSKSLRPSVFRNYFEWRRDRLVAVAAVLAVMLLGVL"
BASE COUNT          444 a         1074 c         1058 g          552 t
ORIGIN      
        1 cacaagggaa atatgcaccc tctgccggtt cgggcaaact gggtggtctg gaatcggcac
       61 acagccgggc cgggaatcga ggacggagcc atgtgcggaa tcgttggagc agtcgccgag
      121 cgtaacgtcg tgcctatcct gatggagggg ctgcggcgac tggagtatcg cggctacgac
      181 tcggccggca tcgcggtgct caatggctcg aagcacctca cgcggctgcg cacggtcggc
      241 aaggtgcgca tgctcgatga ggcgctcgag cagactccca ccgacggcaa gatcggcatt
      301 gcgcacacgc gctgggcgac ccacggcgtg ccgagcgagc gcaacgccca tccgcacatc
      361 tcgcacgatg ggctcgccat cgtgcacaac ggcatcatcg agaaccatga ggagctgcgc
      421 gccgatctgc tgcgccgcgg ctatcagttc gcctccgaga ccgacaccga agtcatcgcg
      481 caccgcgtgc acctgcacct gcagacgctg cccgacctgt tcaaggcggt gcgcgcgacg
      541 gtggccgagc tcgaaggcgc gtatgcgctc gtggtggtga gtgagcacga cccgcagcgg
      601 ctcattctcg cgcgtcaggg ctgcccggtg gtcatcggcc tcggcgtcga cgagaacttt
      661 gtcgcttcgg acgtcgcggc gctgctgccc gtgacgcggc gcttcatctt cctcgaggag
      721 ggtgatgtcg cggaggtgcg ccgtaccgcg gtcaccatca tcgatcgcga cggtgcgctc
      781 gtcacgcgtc cggtgcgcga gagcgagctg tccgccaacg ccgccgagcg cggaccctac
      841 cagcacttca tgctcaagga gatccacgag cagccgcgcg ccatcgccga cacgttgcag
      901 gagcgcgtcg ccaacggccg gctgctcgag gcggccttcg gtccggccgc cagcggggtc
      961 ctcgcccgta cccgcaacgt gcacatcgtc gcctgcggca ccagctatca cgcggcttgc
     1021 gtcgcgcgct acttcatcga gcagatctcc aaggtgccgt gcgccgtgga tatcgcgagc
     1081 gagtaccgct accgcaagcc gctcgtgccg ccgaattccc tgttcgtgac catttcccag
     1141 tcgggcgaga ccgccgatac gctggcggcg ctgcggctcg cgcgccattc gggctacctc
     1201 tcctcgctcg ccatttgcaa cgttcccgaa agctcgctgg tgcgtgaatc ggagctcgtg
     1261 atgctgacgc gcgccggtcc cgagatcggc gtcgcctcca ccaaggcctt caccacccag
     1321 ctcacggcgc tcggcatgct ggtggtggcg ctcgcgcgct atcagggctc cgatgccgag
     1381 cgcgagcgtg ccctcgtcag ggggctgatc gagctgccgg cgatggtgga gcgggctctg
     1441 gagctcgatc cggtggtcca ccgcctggcg gagcgcttcg ccgacaaacg gcacgcgctc
     1501 ttcctcggcc gtggcgcgct gtatcccatc gcactcgagg gcgcactcaa gctgaaggaa
     1561 atttcctata tccacgccga gggctatccg gcgggcgagc tcaagcacgg gccgctggcg
     1621 ctggtcgatg ccgacatgcc ggtggtagcc gtcgcgccga acaacgatct gctcgagaag
     1681 ctcaagtcca acctcatgga ggtacgggcg cgcggcggcg agctgttcgt gtttgccgat
     1741 cccgaggcgc gaatctccgc gagcgatggg gtcacggtca tcgagatgcc gcggcaggtc
     1801 acctacgtgc agtcgccggt ggtgtacagc attccgctgc agctgctcgc ctaccatgcg
     1861 gcgattctca agggcaccga cgtcgaccag ccgcgcaatc tcgccaagag cgtgaccgtc
     1921 gaataacggg caccgcgctg ctgccggcgg gcgccgccgg cggttggttt ctttgcggta
     1981 ggatgccgct gggatgacaa gaacaaccgt ccccgacatc ctggcgggtc tcgcgatcgc
     2041 aggactactg ctaccggagg ccgtggcgta ttcgggtctt gccggcctgc cgccacaggc
     2101 gggagtgatc gcgcttttcg ccgggctcat ctgctacggt gccatcggcc agagccgcgc
     2161 cgccatcgtg agtgcgacat cctcctccgc ggccgtgctg gcttcggcgc tggtggcgct
     2221 cggcgccagc agcgtggccg agcgtgtggc gctcgcgtcc atcctggtag ccagcagcgg
     2281 cgtttggttt gcgttgtgcg cggtgctgcg actcggagcg atgtccaacc tcatcgcccg
     2341 ccccgtgtta cgcggctaca ccttcgggct ggcgctggtc atcgccgtgc ggcaatggcc
     2401 gcacctcgct aatgtgccca cgcacagcag cggcttcttc gccctcatcc tggagctgct
     2461 gcgtagctac cgcgcctggc agctgccgag cctggcgtgc ggtctcggcg cgctcgcggg
     2521 cctgttcctg ctcgagcggg tgccgcgcgt accgggcgcg ctggtggtga tcgtggcgag
     2581 cattctggcg gcgccgctgc tcgccgcgca cgcggtcgcg ctcaccgggc ccatcgagct
     2641 gcatctcacg ctgccggcgc tgagcgtgcc gccgggaata cacacgctgg cgctgctcga
     2701 gtattcgctc gcgctcatgt tcattctgta cgccgagtcc tatggctcga tccgcacctt
     2761 tgcgctgaag cacgatgagc cggtgcagcc caaccgcgat ctcctcgcgc tcggggtggc
     2821 gaacctgctg tcgggactgc tgcgcgctac gccggttggc gccggctact ccgcgacttc
     2881 cgccaacgat gccgccggcg cgcgctcgcg cctggccggg ctcagcgcgg ccgcagtcgt
     2941 gctgctgttg gtgctggcgt ttcttccctg gatcgagcgc attccccagc cggtgctggc
     3001 ggccatcgtc attcacgcgg tcagcaagtc gttgcgcccg agcgtgttca ggaactactt
     3061 cgagtggcgc cgcgatcgcc tggtagcggt cgcggcggtg ttggcggtga tgctgctcgg
     3121 ggtcctga
//
LOCUS       DATDMP010000348         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_166447, whole genome shotgun sequence.
ACCESSION   DATDMP010000348 DATDMP010000000
VERSION     DATDMP010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166447"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..516)
                     /locus_tag="VI195_06095"
     CDS             complement(235..516)
                     /locus_tag="VI195_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823992.1"
                     /translation="MLKTSLLAACLIVLAGACASNPTSATRHATANAASNSGSPAGCV
                     NKTATRLPTSPDECAGFGNSHSSDAIKSTGKPQAGDALRLLDPSVTVHP"
     gene            667..936
                     /gene="rpsO"
                     /locus_tag="VI195_06100"
     CDS             667..936
                     /gene="rpsO"
                     /locus_tag="VI195_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY6823993.1"
                     /translation="MALTSEKKGGILAQFRRGPADTGSPEVQVALLSERINGLGEHFT
                     AHKRDHSSRRGLVKLVNQRRKLLDYLKSTRPEKYQELVERLGLRR"
     gene            1011..>2317
                     /gene="pnp"
                     /locus_tag="VI195_06105"
     CDS             1011..>2317
                     /gene="pnp"
                     /locus_tag="VI195_06105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY6823994.1"
                     /translation="MSVVSKSFPYGPHSVTIEAGQLARQADGSVRVSMGETVVLVTAV
                     AKQTPTPGRDFFPLTVNYVEKTYAAGRIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PDGFYNDVQVVATVLSMDPEIDADVPSLIGASAALALSGIPFNGPVGAARVGWKDGRY
                     QLNPTAKELSQSQLHLVVAGTEQAVLMVESEAKGLSEEVMLGAVVFGHEQMQVAIRAI
                     RELANEAGKPRWKWEPPADNAELNSAVSLHAQAALAQAYSITEKQQRHARLGEIKAQT
                     VTTLTGGEQPKFTAEHVESALFKLESNIVRQRILNGEPRIDGRDTRTVRPISIKVGVL
                     PRTHGSALFTRGETQAMVVTTLGTTRDAQIIDALEGERREPFMLHYNFPPFSVGETGM
                     LGSPKRREIGHGNLARRGVNAVMPDMSAFPYVIRVVSEILESNG"
BASE COUNT          428 a          721 c          778 g          390 t
ORIGIN      
        1 gaggaacaaa ggttcctggg tatcggtgag accgacatga ccggcgcact gcacccgcga
       61 cggctcctca acctcgagtc ggccgtagcc ggcgagggtg gcggtattta gccgggcgag
      121 ggtggcagtg tgtagcgggg cgagcgtggc agtgtgtggg gtgttcaccc caatagttca
      181 ccggatggtt aactattgct ccgaacacct atgaagcgcg cgcctgaagc gcgctcaagg
      241 atggacggtg accgaaggat cgagcagccg cagtgcgtcg cccgcctggg gcttgccggt
      301 gctcttgatg gcatctgaag agtggctgtt gccaaagccg gcgcattcat cgggactcgt
      361 gggcagccgc gtagcggtct tgttgacgca gccggcgggt gagccggagt tgctggcggc
      421 gttcgcggtc gcgtggcgtg ttgcgctcgt ggggttactg gcacaggccc cggcaagaac
      481 gatcagacaa gcagcaagga gcgatgtctt caacatggcc gtgtcacctc gggaccatcg
      541 taggcctcaa cgtgcgggtc gtgcaacggt gcacaagctt ttcggcttgt ggcgaggcca
      601 cgggcgcagg tagaatgccc ggctcacaaa agggctctta agacaagtag ttaggacaat
      661 cgcgcaatgg ctttaaccag tgagaaaaag ggcggaatcc tcgcgcagtt ccgccgcggt
      721 ccggccgaca ccggatctcc cgaagtgcag gtcgcgctgc tctccgagcg catcaacgga
      781 ctcggcgagc acttcaccgc acacaagcgt gaccactcct cgcgccgcgg gctggtgaag
      841 ctcgtcaacc agcgacgcaa gctcctcgat tacctgaagt ccacacgacc ggagaagtac
      901 caggagctcg tcgagcgcct cggacttcgc cgctaagact tgagggccgc agttaagcgg
      961 cctttgcgtt ttcggccccc gggattttca cttttttcga ggattcagct gtgagcgtcg
     1021 tcagcaagtc gtttccgtac ggcccgcatt cggtaaccat cgaggccggg caactcgccc
     1081 gtcaggccga tggctcggtg cgcgtatcga tgggcgaaac cgtggtgctc gtcaccgcgg
     1141 tcgccaagca gaccccaacc ccgggccggg atttcttccc gctcacggtg aactacgtcg
     1201 aaaagaccta tgccgccggg cgcatccctg gcggcttctt caagcgcgaa gggcgcccga
     1261 gcgagaagga gacactcact tcgaggctga tcgaccgccc gatccgaccg ctgttcccgg
     1321 atggttttta taacgatgtg caggtggtcg ccaccgtgct gtcgatggac ccggagatcg
     1381 acgcggatgt cccttcgctc atcggcgcct cggcggcgct ggcgctctcg ggaattccat
     1441 tcaacgggcc ggtgggtgcg gcgcgcgtcg gctggaagga cggccgctat cagctcaacc
     1501 cgaccgccaa ggaactcagt cagtcacagc tgcacctcgt cgtggcgggc accgagcagg
     1561 cggtgctgat ggtggagtcc gaagccaagg gactctcgga agaggtcatg ctgggtgcgg
     1621 tggtgttcgg ccacgagcag atgcaggtcg ccatccgcgc aatccgtgag cttgcgaacg
     1681 aggcgggcaa gccgcgttgg aagtgggagc cgccggcgga caacgccgag ctcaacagcg
     1741 cggtgtcgtt gcacgcgcag gcggcgctcg cccaggccta cagcatcacc gagaagcagc
     1801 agcggcatgc gcgcctcggc gagatcaagg cgcagaccgt gaccacgctc accggcggtg
     1861 agcaacccaa gttcaccgcg gagcatgtcg agagcgcgct cttcaagctc gagagcaaca
     1921 tcgtgcggca gcgcatcctg aacggcgagc cgcgcatcga cgggcgcgac acccggaccg
     1981 tgcggccgat cagcataaag gtcggcgtgc tgccgcgcac ccatggctcg gcgctgttca
     2041 cccgcggcga gacgcaggca atggtggtga ccacactcgg taccacgcgc gatgcgcaga
     2101 tcatcgatgc cctggagggc gagcgccgcg agcccttcat gctgcactac aacttcccgc
     2161 cgttctcggt gggtgagacc gggatgctcg gctcgcccaa gcgccgcgag atcggtcacg
     2221 gcaacctcgc gcgtcgcggc gtcaatgcag tgatgcccga catgagcgcg ttcccctacg
     2281 tgatccgcgt ggtgtcggag atcctcgaat ccaacgg
//
LOCUS       DATDMP010000349         4407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_944129, whole genome shotgun sequence.
ACCESSION   DATDMP010000349 DATDMP010000000
VERSION     DATDMP010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944129"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..364)
                     /locus_tag="VI195_06110"
     CDS             complement(95..364)
                     /locus_tag="VI195_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823995.1"
                     /translation="MKRAAFALGTLAAATIAMAQTSPPPEPPTSTAPQEETAPPRDAS
                     PRMSETDKQTLMQDCVRQVQAANPSVPEKDVKDYCDKQVKSYSPH"
     gene            603..812
                     /locus_tag="VI195_06115"
     CDS             603..812
                     /locus_tag="VI195_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823996.1"
                     /translation="MLRRLSNSPAQFSGSMRKLGAYAAVALLVPGGSVIAFVLWTSQR
                     GWLTPRTWRALFALAALGTGLIVPG"
     gene            1025..3016
                     /locus_tag="VI195_06120"
     CDS             1025..3016
                     /locus_tag="VI195_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6823997.1"
                     /translation="MTLQTHSYRQVGSCCALILLTGVVSLARAADPQPYHVDFSSTGI
                     GEVDSTLKATSQLATLRTSAPVDPFGLIARARSDIGRLTTVLESFGYYQSSMSITING
                     LALDDPQLGDTLTALPQGKDAACRVSPTLGPLFHLGTITIDGSVPDSARSSLELASGA
                     PAVANDVLAGGARLLTALENQGYAFAKVDPPVAREDPQQHVLNLTFHVVTGPHVQIGE
                     IRFEGLKRVNERLVRRRLLLHTGEPYSALAVEHARKDLLVLGVFASVSVHLADSADNE
                     GRVPITFQLEERRRHAVSLSGGYSSDLGGSAGVTWTDRNVRGNAEQLTLAATVTNLGG
                     TASTGIGYDTSAKYLIPEFAHRDQSLQFAVGAIRQSLQAYDQKALTSGVTLSRKLSTV
                     WTATIGVSAIHETITQEGLTATYTLFGLPMGILYDSTDLPSPLSDATHGVRAALTVTP
                     TRSQGELDKTFYITQFSIADYLDLHRLFRTDRGRSVLALRAILASAPGATTTSNTITD
                     SSCPPTNPPPQCTVSVPDIPPDQRFYAGGSGTVRGYRYQSVGPEFPDGNPIGGTALTA
                     LNVEFRQRFGRNFGAAVFVDAGVVGEKLSPLSGLFHGGRCSASTAAQGTTDCWAVGVG
                     AGPRYYTPIGVLRLDIAVPTYRRPNDDRFELYIGLGQAF"
     gene            3016..>4407
                     /locus_tag="VI195_06125"
     CDS             3016..>4407
                     /locus_tag="VI195_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6823998.1"
                     /translation="MRRALRIGAWTLGSLLGLCLALVAAVLVAGNTAGGRALIERTIT
                     RFSDGHLRLAGLAGSFPAAIDLAQLQLADEHGVWLTAQGISLRWSPLALLARHLKVER
                     LHFARVDIERRPVSHASQSSGTSVPHTDIGELSIDALELGPQLAGLRASLSVRGDAHL
                     RSLADADASLIAHRTDAPGDYQLKLRSDAARLDGSLVLKEHAGGALANLLQVPALGEV
                     SVEAKLNGPRNAALLHLAASAGELTALAQGSLDLTREAADLTYRLDAAAMAPAAELSW
                     HRIALEGHWQGTLDAPRADAHLEIDRLQVPGNSALKNLTANLSAEGGELALHALAEGL
                     VVPGPQPRLLTDDPLRIDARVRLKEPSRPLELTADHRLFSLQAQATTAAPMRTTFALR
                     LPDLTPLAVLAGEKLRGKADLKGNVEASSARTHLELEASTEVANGAGLVSSMLAGTSH
                     LQLAAAMTDRTLEV"
BASE COUNT          737 a         1500 c         1350 g          820 t
ORIGIN      
        1 aaccaaatgg tgaactatca ccggaggcac ctgctacagc gagctctcct tcgtgggcgt
       61 gcccaccgct ctcaattgag gccgtcagtc agcgttaatg cggtgagtaa ctcttcacct
      121 gcttatcgca gtaatccttg acgtccttct ccggcacgct cgggttggcg gcttgcacct
      181 gccgcacgca atcctgcatc agagtctgct tgtcagtctc actcatgcgg gggctcgcgt
      241 ccctcggggg agcggtctcc tcctgcggag cggtgctcgt gggcggctcc ggcggcgggc
      301 tcgtctgcgc catggcaatg gttgcggcgg ccagcgtgcc taatgcaaat gcggctcgtt
      361 tcacggtgca cctcctttcg ttacagaaag cagcctagac acacgctccg gatgacacta
      421 tcggagaggg cctacatgtc tttcctgaca acgcgtatcg cgctgtatcc gcagccaagg
      481 cagtatgttg tcgccaacca aagacacgcc tcggctaatg aacgttccgt gcacatggag
      541 ttcacgtgca gttcagccgc ggtgatgtat ctggatagag aggcgacatt cgaaaggaag
      601 cgatgctgcg tcgactcagc aattctccgg ctcaattctc cggctcgatg cgcaagctgg
      661 gagcgtacgc ggccgttgcg ctgcttgttc ccggcggcag cgtgattgca ttcgttctgt
      721 ggacttctca gcgcggatgg ctgacaccgc gcacctggcg tgccctgttt gccctggcag
      781 cccttggcac cggcctcatc gttccaggct aaacgtagca gcgacgcctt tcggcgacac
      841 gctgccattt aatcaaacac cgtcttcgct cccggcgacc gcagcgccgg ctgaaattgc
      901 cacgcttcag ccacgagtca aatcgcctac cccggctgac attaagttcc tcaaagacca
      961 gccacaatcc gcaagcatgg gtgtagtcga gcaagcataa ggcgcgtcgg taccgacctt
     1021 tggaatgacg ctgcaaacac acagctatcg ccaggttggt tcgtgctgcg ccctcatctt
     1081 gctgacgggt gtcgtttccc tcgcgcgggc cgctgacccg cagccgtacc acgtggactt
     1141 ctcatccacc ggaattggcg aggtcgattc gacgctcaag gcgacctcgc agttggcgac
     1201 gctgcgcact tcggctcccg tcgatccgtt tggcctgatc gcccgcgcca ggagtgatat
     1261 cggtcgcctc accacagtgc tcgagagttt cggttactac cagagctcca tgtcgataac
     1321 tatcaacggt cttgcactcg atgacccgca actcggcgac accctgactg cgctgccgca
     1381 aggaaaggat gcggcgtgcc gggtctcacc cactctcggt cccctcttcc acctcggcac
     1441 catcacgatc gatggctcgg tcccggactc cgcgcgcagc agcctcgagc tcgcctcggg
     1501 cgcaccagcg gtagccaacg atgtgttggc gggtggcgcg cgcttgctca cggcgctcga
     1561 gaaccagggc tatgctttcg ccaaggtcga tccgccggtc gcccgcgagg atccgcagca
     1621 gcatgtactc aatttgacct ttcacgtcgt gactggccct cacgttcaga tcggcgagat
     1681 acggttcgag ggactgaagc gcgtgaacga gcggctggtg cgccggcgtc tgctgctgca
     1741 taccggcgag ccctacagcg cgctcgccgt cgagcatgcc cgcaaggatc tgctcgtact
     1801 gggagtgttc gcctcagtca gcgtgcacct cgccgactcc gcggataacg aaggccgcgt
     1861 gcccatcacg ttccagctgg aggagcggcg gcggcatgcg gtgagtttga gtggcgggta
     1921 ctcgagcgac ctcggtggca gtgccggcgt cacctggacc gaccgcaacg tacgcggcaa
     1981 cgccgagcag ctcactctcg cggccacggt gaccaatctc ggcggcaccg cctcgacggg
     2041 catcggctat gacacgagcg ccaaatacct catccccgag ttcgcgcatc gcgaccaatc
     2101 gttgcagttt gcggtgggcg ccatccggca gtcgctgcag gcgtacgatc agaaagcgct
     2161 cacctccgga gtcaccctca gccgcaagct gtccacggtc tggaccgcaa ccatcggggt
     2221 ctcggccatc cacgagacga ttactcagga agggctcacc gcgacgtaca cgctgttcgg
     2281 tctgccgatg ggtattctct atgactcgac ggatcttcca tcgccgttga gcgatgcgac
     2341 ccatggcgtg cgcgccgcac tcacggtcac tccgacccgc tcgcaggggg agctcgacaa
     2401 gaccttctac atcacgcaat tcagcattgc cgactatctg gatctgcacc ggctcttccg
     2461 caccgaccgc gggcgcagtg tgctggcgct gcgcgctatt ctcgccagtg cacccggagc
     2521 caccacgacc tctaacacca tcaccgactc atcctgtccg cccaccaacc cacctccgca
     2581 atgcacggtc agtgtccccg atattccgcc cgatcagcgc ttttacgctg gcggaagcgg
     2641 aacggtgcgc ggctatcgct atcagtccgt cggccccgag tttcccgatg gcaatcccat
     2701 aggcggcact gcactcaccg cgctcaacgt cgagttccgc cagcgcttcg gcaggaactt
     2761 tggcgcggcg gtgtttgtcg acgctggtgt ggtgggtgag aaactgagcc ccctgtcggg
     2821 tctcttccat ggcggtcgct gcagcgcgag cacggccgcg cagggcacga cagactgctg
     2881 ggcggtaggc gtcggcgcgg gcccacgcta ttacactccg attggtgtgt tgcgactcga
     2941 tatagccgtg ccgacctacc gccgtccgaa tgatgatcgg ttcgagctct acatcggctt
     3001 ggggcaggcg ttctgatgcg tcgcgccctg cgcatcggtg cctggacgct cggctcgctg
     3061 ttgggcctgt gcctcgcgct cgttgcagcg gtgctggtgg cgggcaacac cgcgggcggc
     3121 cgcgccttga tcgaacggac gatcacccgc ttctcggacg gtcacctgcg cctcgcgggt
     3181 ctagccggct cctttccagc ggcgatcgac ctcgcgcagc tccagctcgc cgacgagcac
     3241 ggcgtctggc tcaccgcgca gggcatctcg ttgcggtggt cgccgcttgc gctgctggca
     3301 cggcatctga aagtcgagcg cctgcatttc gcgcgagtcg acatcgagcg tcgacccgtg
     3361 agccacgcgt cgcagtcgag cggaacgagt gtgcctcaca ccgatatcgg tgagctgtcg
     3421 atcgatgccc tcgagctcgg acctcaactt gcgggtctgc gcgcgagtct ttccgtgcgg
     3481 ggggacgcgc acctgcgatc gctcgccgat gccgacgcga gcctcatcgc gcatcgcacc
     3541 gacgccccgg gcgattatca gctgaagctc cgctccgatg ctgcgcgatt ggatggcagt
     3601 ctcgttctca aggagcatgc cggcggggcg ctcgcgaatc tgcttcaggt gccggcgctc
     3661 ggcgaggtgt cggtcgaggc gaaactcaat gggccgcgta acgctgcact cctccacctc
     3721 gcggcgagcg ccggagaact cactgcactc gcgcagggta gcctcgatct gacccgagag
     3781 gccgcggacc tcacctatcg gctcgatgcc gcggcgatgg caccggccgc ggagctctcc
     3841 tggcaccgca tcgccctcga gggacactgg cagggaacgc ttgatgctcc gcgcgccgat
     3901 gcgcatctcg agatcgatcg gctgcaggta cccggtaaca gcgcgctgaa aaacctcacc
     3961 gccaacctga gcgcggaagg aggtgagctc gcgctgcacg ccctggccga gggcctggtg
     4021 gttcccgggc cgcaaccgcg cttgctcacc gatgatccgc tgcggatcga tgcgcgtgtg
     4081 cgcctcaagg aaccctcgcg cccgctcgag ctgacggcgg accatcggct cttctcgctg
     4141 caggcgcagg ccaccacggc cgctccgatg cgcacgacct tcgcgctgcg cctgcctgat
     4201 ctcaccccgc tcgccgtgct cgccggtgag aagctccgcg gcaaagcgga tctcaaaggg
     4261 aatgtcgagg caagctccgc acgcacgcat ctcgagctcg aagcgagcac cgaggtggcg
     4321 aacggagcgg gcctcgtgag ctcgatgctc gcggggacct cgcacctgca gctcgcggcc
     4381 gccatgaccg atcgcacact cgaggtc
//
LOCUS       DATDMP010000350         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_56105, whole genome shotgun sequence.
ACCESSION   DATDMP010000350 DATDMP010000000
VERSION     DATDMP010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56105"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /gene="hslU"
                     /locus_tag="VI195_06130"
     CDS             <1..443
                     /gene="hslU"
                     /locus_tag="VI195_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEY6823999.1"
                     /translation="HVLFIASGAFSLSKPSDLIPELQGRLPIRVELDSLKVGDFVRIL
                     TEPDASLTTQYKALMGTEGVQVEFATDGVRRIAEIACQVNERTENIGARRLHTVMERL
                     LESVSYEAAEYAGNGKGTLTIDARYVDAHLGKLVQDEDLSRYIL"
     gene            complement(477..1247)
                     /locus_tag="VI195_06135"
     CDS             complement(477..1247)
                     /locus_tag="VI195_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6824000.1"
                     /translation="MNTAHLIISRRTIITGLASSAGLALAGCSETQPPTYGNVLRMAD
                     NFTYRAQRLLLPRQSLAREYERRDITSIPAIGTTDPADPHQGNYNEKSGVIYQQLRNG
                     QFADWRLRIEGSVSRPGSFSLAELQRFPARTQITKHTCEEGWSAIAAWTGVPLRRVLE
                     AAGIRPSARFVNLYAFDDAAESIDMLDALHPQTILAYGMNGRELPIAHGAPLRLRVER
                     QLGYKSLKFLHRIVVTDRFEDLGKISEPHNGWAWYVGI"
     gene            complement(1244..2017)
                     /locus_tag="VI195_06140"
     CDS             complement(1244..2017)
                     /locus_tag="VI195_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY6824001.1"
                     /translation="MSTNNFDPPSARAGAGHTRWVRVTHWIVAASVLTLAVSGFTILM
                     AHPRLYWGAVGNDLTPALIELPVSRNYRHGGWEVATTNFPDGGRAVSAIRTYDILNQN
                     GWARSLHFLAAWFLVVTGTIYLLGGILSGHLRRDLLPRRVELTARRLWDDLTAHLRWR
                     GQSVPGGLPYGLLQKSSYCAVVFVGLPLMALTGWAMSPAMNAAYPFLAGLFGGTQSAR
                     TIHFCVFAALLLFVIVHLVMVGLSGFKRQMRAMTWGRPT"
BASE COUNT          375 a          690 c          699 g          351 t
ORIGIN      
        1 atcacgtgct gttcattgcc tcgggtgcct tcagcctgtc gaaaccctcc gacctcatcc
       61 ccgagctgca gggacgcctg ccgatccgcg tggagctcga ctcgctcaag gtcggtgact
      121 tcgtgcgcat cctcaccgaa cccgatgcct cgctcaccac acagtacaag gccctcatgg
      181 gcaccgaggg cgtgcaggtg gagttcgcca ccgatggcgt gcggcgtatc gccgagattg
      241 cctgccaggt gaatgagcgt accgagaaca tcggcgcgcg ccgcctgcac accgtgatgg
      301 agcggctgct cgagagcgtc tcttacgagg cggccgaata cgccggcaac ggcaaaggca
      361 cgctcaccat cgatgcccgc tacgtcgacg cccacctcgg caagctggtg caggatgagg
      421 acctgagccg ctacatcctc tagcccttac cctctagccc tgcgagcctc gcgggttcag
      481 atgccgacgt accaggccca gccgttatgc ggctcgctga tcttccccag gtcttcgaac
      541 cggtcggtga ccacgatgcg gtgcagaaac ttcaggctct tgtagccgag ctgcctctcc
      601 acgcgcaggc gcagcggtgc accgtgcgcg atgggaagct cgcggccgtt catgccatac
      661 gcgaggatcg tctgtggatg cagagcgtcg agcatgtcga tgctctcagc cgcgtcatcg
      721 aacgcataga gattcacgaa ccgcgcgctc ggccggatgc cggccgcctc cagcacccgc
      781 cgcagcggca cacccgtcca ggcggcaatc gccgaccatc cctcctcgca ggtgtgcttg
      841 gtgatctgcg tgcgcgcggg gaagcgctgc agctcagcca gcgagaagct ccccggccgg
      901 ctcacactgc cttcgatccg cagccgccag tcggcgaact gaccgttgcg caactgctgg
      961 tagatcactc cgctcttttc gttgtagttc ccctggtgcg ggtccgccgg atcggtcgtg
     1021 ccaatggcgg gaatcgaggt gatgtcgcgc cgctcgtact cgcgggcgag cgactgacgc
     1081 ggcagcaaaa ggcgctgcgc gcggtaggtg aagttgtcgg ccatccgcag cacgttgccg
     1141 taggtcggcg gctgcgtctc cgagcagccg gcgagcgcga ggcccgcgct cgatgcgagt
     1201 ccggtgatga tggtgcggcg gctgatgatc aggtgtgcgg tgttcatgtg ggccttcccc
     1261 aggtcatggc gcgcatctgc cgcttgaatc cggacaggcc gaccatcacc aggtggacga
     1321 ttacaaagag aagcagcgcg gcgaacacac agaagtgaat ggtgcgggcc gactgcgttc
     1381 cgccgaagag cccggcgagg aacgggtagg cggcattcat ggccggagac atcgcccagc
     1441 cggtgagcgc catgagcggc aggccgacaa acaccacggc acagtagctg gatttctgca
     1501 gcagtccgta cggcaggccg cccggcacgg actgcccgcg ccagcgcagg tgagcggtga
     1561 ggtcatccca gaggcgtcgc gccgtcagct cgacgcggcg aggcaggaga tcacgccgca
     1621 ggtgaccgga gaggatgccg ccgaggagat agatcgtccc ggtcacgacc aggaaccatg
     1681 cggcgaggaa gtgcagactc cgcgcccagc cgttctggtt cagtatgtcg tacgtgcgga
     1741 tggcgctcac ggctctcccg ccgtcgggaa agttcgtcgt ggccacttcc catccgccgt
     1801 ggcggtagtt gcggctgacg ggcaattcga tcagcgcggg agtcagatcg ttgccgaccg
     1861 caccccagta gagccgcggg tgagccatga ggatcgtgaa gccgctgacg gcgagcgtca
     1921 gtacgctcgc ggcgacgatc cagtgcgtga ccctgaccca gcgggtgtgg cctgcgccag
     1981 cgcgggcgga tggtggatca aaattattcg ttgacatcga gatctccgcg cagcgcggat
     2041 ccccctgcga tgcacgggaa agtctaactc cgattcgagt gcgcctgtcg actcacacgc
     2101 gaacgtagcg ctcta
//
LOCUS       DATDMP010000351         3507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_500839, whole genome shotgun sequence.
ACCESSION   DATDMP010000351 DATDMP010000000
VERSION     DATDMP010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500839"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VI195_06145"
     CDS             complement(<1..228)
                     /locus_tag="VI195_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY6824002.1"
                     /translation="MRRAGGGRPPAAERLQKVLAQAGLASRREAEEWIRAGRITINGA
                     VAVLGARVGAGDELRLDGRPLRVHAAGHARAF"
     gene            complement(229..927)
                     /gene="scpB"
                     /locus_tag="VI195_06150"
     CDS             complement(229..927)
                     /gene="scpB"
                     /locus_tag="VI195_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY6824003.1"
                     /translation="MNESYIRNVIEAALLAAGGPLPVAELLRLFDDAVRPSAQAVRAA
                     LHSLGAEYAGRGIELKETASGFRIQVRRELALEISRLWPERAARYSRALLETLALIAY
                     RQPITRGEIEAVRGVTVNPNIIRTMIERNWVRVVGHRDVAGHPELLGTTREFLDYFGL
                     KTLDELPPLTELKAMGDLTLQLALADEPVAAAPPAPGDIESGAVEDPQEDDELSADGS
                     GSAELVAGPRALDD"
     gene            complement(924..1874)
                     /locus_tag="VI195_06155"
     CDS             complement(924..1874)
                     /locus_tag="VI195_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY6824004.1"
                     /translation="MKQRPELRLVPRAPVTPPAPELPPEQVEMPFAVVNGEPISQLPR
                     DLYIPPQALEVFLEAFEGPLDLLLYLIRRQNLDILDIPLAEITRQYMQYIELMQELQL
                     ELAGEYLVMAATLAEIKSRMLLPRPKLAEDSEEDPRAELVRRLQEYERYKRAAEQIDS
                     LPRLERDVWSVSAELKERRVVRSLPQITLQELLIAFKEVATRAEMFAHHHIQREQLSV
                     GERMSDILAALGVGGFVPFMQLFRPQEGRMGVTVTFVAILELMREGLIDIVQAQPYAP
                     LHVRATGPVRALHAVADNPPAGAPEHAADAGALPQDEDVS"
     gene            complement(1887..3146)
                     /locus_tag="VI195_06160"
     CDS             complement(1887..3146)
                     /locus_tag="VI195_06160"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY6824005.1"
                     /translation="MRYNSRRESFCHCTHVSSPAPARRVLSGMRPTGPLHLGNWHGAL
                     RNWVELQYQYECFFFVADWHMLTTGYEDTSHLQENIREVLIDWLAAGLNPGVATLFIQ
                     SHVPEHAELHLLLSMIMPLGWLERVPSYKDQQEQLKEKDLATYGFLGYPLLQSADILV
                     YRAAYVPVGEDQVAHVELTRETARRFNHLYGREPDFEAKAERAVKSLGGRNSGNYRQL
                     RRRFQESGDTEALQRAQALVQSNNRITLADRERLLGFLEGTGVAILPEPQALLTATPK
                     VPGLDGRKMSKSYGNTIGLREDPEAVAHKLRAMTTDPARVRRNDPGDPEKCPVWDLHK
                     IYSDEATRSWVNEGCRSAGIGCLDCKQPLIERVVEEVSGMRRRAQEFEDNPELVRNII
                     GEGADKAREAARATLEDVRRAMHLRPG"
     gene            complement(3136..>3507)
                     /locus_tag="VI195_06165"
     CDS             complement(3136..>3507)
                     /locus_tag="VI195_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEY6824006.1"
                     /translation="MLRACLPGPYTFLLRATRATPRRLQHERRRTIGVRIPDHPVPRL
                     LLAELGEPLMSSTLLLPADERPLTAGREIEARLGHDIDAVLDGGDCGIEPTTVIDLSV
                     TPPLIVRKGKGALGPITGRAL"
BASE COUNT          546 a         1200 c         1185 g          576 t
ORIGIN      
        1 gaatgcgcgc gcgtgccccg ctgcatgcac acgcagcggg cgtccatcga gccgcagctc
       61 gtctcccgcg ccgacgcgcg ccccgagtac cgctacggcg ccgttgatgg tgatgcggcc
      121 ggctcggatc cactcctccg cttcccgcct cgatgcgagg ccggcctgcg caagcacttt
      181 ctgcagccgc tcggcagccg ggggccgacc gccgcctgcg cggcgcattc agtcgtccag
      241 cgcgcgcgga cctgcgacga gctcggccga gccgctgccg tccgccgaga gctcatcatc
      301 ctcctgcggg tcttcaactg cgcccgattc gatgtcgccg ggcgcggggg gtgccgcggc
      361 taccggctcg tcggcgagcg caagctgcag ggtgagatct cccatggcct tgagctccgt
      421 gagcggtggc agctcatcga gggtcttcaa gccgaagtag tcgaggaact cgcgcgtggt
      481 gccgagcagc tccggatggc cggcaacgtc acggtggccg acgacgcgca cccagttgcg
      541 ctcgatcatg gtgcgaatga tgttgggatt gacggtgacg ccgcgcaccg cttcgatctc
      601 gccgcgcgtg atcggctggc ggtacgcgat cagcgcgagc gtctcgagca gcgcacgcga
      661 gtagcgcgcc gcccgctcgg gccacagccg cgagatctcc agcgccagct cgcggcgcac
      721 ctggatgcga aaaccgcttg cggtctcctt gagctcgatg ccgcgcccgg catattcggc
      781 gccgagcgag tggagcgccg cgcgcaccgc ctgggcgctc ggacgcacgg cgtcatcgaa
      841 caggcgcaac agctcggcca ccggcagcgg gccgcccgcg gcgagcagcg cggcctcgat
      901 gacgttacga atgtacgact cgttcatgaa acgtcctcat cctgcgggag agcgccggca
      961 tcggcggcat gctcgggcgc gccggctggc ggattgtcgg cgacggcgtg cagagcccgt
     1021 accgggccgg tggcgcgaac gtgcagcggg gcgtacggct gcgcctgcac gatgtcgatc
     1081 agtccctcgc gcatgagctc gaggatggcg acgaaggtga ccgtgacacc catgcgcccc
     1141 tcctgcggac ggaacagctg catgaacggc acgaagccgc ccacaccgag cgcggcgagg
     1201 atgtcgctca tgcgctcacc gaccgagagc tgctcgcgct ggatgtggtg gtgggcgaac
     1261 atctccgcgc gggtcgccac ctccttgaag gcgatgagca gctcctgcag cgtgatctgc
     1321 ggcagcgagc gcaccacgcg ccgctccttg agctccgccg acacgctcca gacatcgcgc
     1381 tcgagacgtg gcagtgagtc gatctgctcg gcggcgcgct tgtaacgctc gtactcctgc
     1441 agacgccgca ccagctcggc gcgcggatcc tcctcgctgt cctccgccag cttcggccgc
     1501 ggcagcagca tgcgcgattt gatctccgcg agcgtggcgg ccatcaccag gtattcgccc
     1561 gcgagctcga gctgcaactc ctgcatgagc tcgatgtact gcatgtattg gcgggtgatc
     1621 tccgcgagcg gaatgtcgag tatgtcgaga ttctgccggc ggatcaggta cagcagcaga
     1681 tcgagcggtc cctcgaaggc ctcgagaaat acctcgaggg cctgcggcgg gatgtacagg
     1741 tcgcggggca gctgcgagat cggctcgccg ttcaccaccg cgaagggcat ctccacctgc
     1801 tcgggcggca attccggcgc cggcggcgtc acgggcgcgc gcggtaccag gcgtaattcc
     1861 ggccgctgct tcatgggtcg cgtgcctcag cctgggcgca gatgcatggc gcggcgcaca
     1921 tcctcgagtg tggcgcgcgc cgcctcgcgc gccttgtcgg cgccttcgcc aatgatgttg
     1981 cgcaccagct ccgggttgtc ttcgaattcc tgggcacgac ggcgcatgcc gctcacctcc
     2041 tccaccacgc gctcgatgag cggctgcttg caatcgaggc agccgatccc cgcgctgcgg
     2101 caaccctcgt tcacccagct gcgcgtcgcc tcatcggaat agatcttgtg aaggtcccat
     2161 accgggcact tctccggatc gcccggatcg ttgcggcgca cgcgcgccgg atcggtggtc
     2221 atggcacgca acttgtgtgc caccgcctcc ggatcctcgc gcagcccgat ggtgttgccg
     2281 tacgacttcg acatcttgcg tccatcgagc cctggcacct tgggcgtcgc ggtgagcagc
     2341 gcctgcggct cgggcaggat ggcgaccccg gtgccttcga gaaacccgag caaccgctcg
     2401 cggtcggcga gcgtgatgcg gttgttactt tgcaccagcg cctgggcgcg ctgcagcgcc
     2461 tcggtgtcgc cgctttcctg gaagcgccgg cgcaactggc ggtagttgcc gctgttgcgg
     2521 ccaccgaggc tcttcaccgc gcgctcggcc ttggcctcga agtccggctc gcgtccatag
     2581 agatgattga agcgccgcgc ggtctcgcgc gtcagctcga catgcgccac ctgatcctcg
     2641 ccgaccggca cgtaggcggc gcggtacacg aggatgtcgg cgctctgcag caacggatag
     2701 ccgagaaagc cataggtggc gagatctttt tccttgagct gctcctgctg atccttgtag
     2761 ctcggtacgc gctcgagcca gccaagcggc atgatcatcg acagcagcag atgcagctcc
     2821 gcatgctcgg gcacatgcga ttggatgaac agcgtggcaa cgcccgggtt cagccccgcg
     2881 gccagccagt cgatcagcac ctcgcgaatg ttctcctgca gatgcgaggt gtcctcgtaa
     2941 ccggtcgtga gcatgtgcca gtcggcgacg aagaagaagc actcgtattg atactgcagc
     3001 tcgacccagt tgcgcagcgc gccgtgccag ttgccgaggt gcagcggacc agtgggccgc
     3061 atgccggaaa ggacgcgccg cgccggcgcg ggggaactca cgtgcgtgca atgacagaat
     3121 gactcacgac gcgaattata gcgcacgccc cgtgatcgga ccgagtgcgc ccttgccttt
     3181 gcgcacgatc agcggtggcg tgaccgacag gtcgatcacg gtcgtgggtt cgatgccaca
     3241 gtcaccgccg tcgagcaccg catcgatgtc gtgaccgagg cgcgcctcga tctcgcgtcc
     3301 cgcggtgagt ggccgctcgt ccgccggcaa cagcagcgtc gagctcatga gtggctcacc
     3361 gagctccgcc agcagcagcc gcggcacggg gtgatccgga atacgcacgc cgatggtgcg
     3421 ccggcgctcg tgttgtagac gacgtggcgt cgcgcgcgtc gcgcgcagca ggaaagtata
     3481 tggacccggc aggcatgcgc gcagcat
//
LOCUS       DATDMP010000352         5286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_945868, whole genome shotgun sequence.
ACCESSION   DATDMP010000352 DATDMP010000000
VERSION     DATDMP010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945868"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VI195_06170"
     CDS             <1..386
                     /locus_tag="VI195_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6824007.1"
                     /translation="TAELVKLLEMEEFADRPARGFSQGERLRTALARALIHGPRNLVL
                     DEPTNGLDVMAVRALRRLLQQLRDAGHCVLFSSHVMQEVSALCDEVVVIAHGVVLEQG
                     APEEIRVRTGAASLEEAFVHLIGPR"
     gene            391..1569
                     /locus_tag="VI195_06175"
     CDS             391..1569
                     /locus_tag="VI195_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6824008.1"
                     /translation="MRAVLVVFAKEFRENLRERRTLFSALILGPLLGPLLFAGGLTLR
                     LERGIGESDRPLELAVSHGERAPNLLDYLRARGVTVTPVDYDQVAARGTVRAGREQLV
                     LAVDADFASRLAAGLPAPLELYADASDLAGSGDSARVHALIAQYSALLGRMRLAARGI
                     DPLLVTTIAVEDIDVSTPTTRSVLILGTLSYLVLLTMLMGGMYLAIDATAGERERGSL
                     EPLLTVPVPREQLIYGKILATCAYMVLSLTLTVTAFAVVLRFTGLERFGMSVNFAPGV
                     ALQLILLTLPLVPLGAALMTLVAAYTRSYREAQTYLALLLLVPTLPLAFAGLLGLRPH
                     LALMAIPSLSQHFLITSLLRAEALPASWIALSVGVTLAVAGLLIAVAGGLYRREALLG
                     "
     gene            complement(1542..1976)
                     /locus_tag="VI195_06180"
     CDS             complement(1542..1976)
                     /locus_tag="VI195_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824009.1"
                     /translation="MRAGREVAVPEDVSGALERLLGQRIAHVKVIEHSWFARLHGACA
                     TTRVERIYLRGSAADFFSNPWLMLHEYCHVLRQWQTGSLTLRRYLLECVRRGYWNNRF
                     EVEARAFADQNAPQMHALLAGVAWSWSARPGSETQPSSASRR"
     gene            complement(1983..2435)
                     /locus_tag="VI195_06185"
     CDS             complement(1983..2435)
                     /locus_tag="VI195_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824010.1"
                     /translation="MESREIHVAADHFEVSSGEAHLVTELSSSIAVCLYDAAEEAGAL
                     LHLRCVVKSSKPADVTDTTLATELLLLDRCLQSLRDLAPAARDLQGRILAHLAETPQA
                     RSVYDSVISLVKRFLEDQQVSLLPDDVATGKPRRLRFRPSMGWVHTQE"
     gene            2571..3266
                     /locus_tag="VI195_06190"
     CDS             2571..3266
                     /locus_tag="VI195_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY6824011.1"
                     /translation="MLSCGAAAAALAQTQQPPLSSIRVTGDARVTAKPDRVQIDIGVT
                     TRAALSQDAASQNARAVDGVLAAVRKVAGPTAVLKTISYTLNPNYRYHPKEGEPTIDG
                     YTALNVVEVTLDDLGRIGAVIDAATQAGANRIQGIQFTLRDQDAVRAQALREAATRAR
                     AEVDVLAAALGLKVLRVLTVEESSPRIMPLRVYNGAARAAAAEAAPTPVEAGTLDITA
                     DVTLSVEVGPASR"
     gene            3293..3721
                     /locus_tag="VI195_06195"
     CDS             3293..3721
                     /locus_tag="VI195_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6824012.1"
                     /translation="MISVRQLLDRKGREVFSVEPGAAVLEAIRLMAERHVGALLVMQG
                     DNLAGIISERDYARKVILMGRSSADTPVRDIMTAPVLTVQPETTVEKCMQIMTERRVR
                     HLPVAEGGRVVGMVSIGDLVKAVIAEQQQQIEQLESYIHS"
     gene            3864..>5286
                     /locus_tag="VI195_06200"
     CDS             3864..>5286
                     /locus_tag="VI195_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEY6824013.1"
                     /translation="MAAAPPNPELLQPSSHATRTHGEESGFRLYSLGIDGLLLRLELI
                     DAAHSSLDLQYYVFHGDESGKLVTEALLRAAARGVRVRILVDDGETIRGDEQLYALAG
                     EKNVEIRVFNPWRYRGHNSLLRDIEYLFSHRRLDFRMHNKLFIADRALALLGGRNIGD
                     EYFQVDPSWQFADDDVLTTGPAVQQLAVSFEEYWQSELAIPVQALLPAKATDAAAAAA
                     LAQRVTTPQKAASAAADYEQKLSAQEPLASVLAGRVPLDWAGAEVAADSPYKGKEHAQ
                     NVHVSSLLFAPVAQAIHDTRSELVMVSPYLVPSPHEMRLLEDLRARQRTVRILTSSLE
                     AESSHAAQAGYMHYRKPLLDAGVQLYEIRARPENPRGTGQSEKLSRHGHYSLHAKLLV
                     FDRSVLYIGSMNYDQRSRRLNTEDGLIIHSEDLAGQAARRFDLLTQPQNAYAVHLQEK
                     VPGDAPQITWSTIESGRPVTLYDE"
BASE COUNT          824 a         1829 c         1764 g          869 t
ORIGIN      
        1 gcaccgcgga gctcgtgaag ctgctcgaga tggaggagtt cgccgaccgc ccggcgcgcg
       61 gcttctccca gggcgagcgg ctgcgcaccg cgctcgcacg cgcactcatc catgggccgc
      121 gcaacctggt gctcgatgag ccgaccaacg ggctcgatgt catggcggtg cgcgcgctgc
      181 ggcggctgct gcagcagctg cgcgatgcgg ggcactgtgt gcttttctcc agccacgtga
      241 tgcaggaggt gagcgcgctg tgcgatgaag tcgtggtgat cgcccacggg gtggtgctcg
      301 agcagggcgc gccggaggag atccgcgtgc gcaccggcgc cgccagcctg gaggaagcgt
      361 tcgtgcacct catcggtccc cgctagcctc atgcgcgcgg tactcgtcgt tttcgccaag
      421 gagtttcgcg agaacctgcg cgagcggcgc acgctgttct cggcgctgat cctcgggccg
      481 ctgctcgggc cactgctgtt cgccggcggc ctcacgctgc ggctcgagcg cgggatcggt
      541 gagagcgacc ggccgctcga gctcgccgtg tctcatggag agcgcgcacc gaacctgctc
      601 gactacctgc gcgcgcgcgg cgtgacggtg acgccggtcg attacgatca ggtggcggct
      661 cgcgggacgg tgcgcgccgg acgcgagcag ctggtgctcg cggtcgatgc ggactttgcc
      721 agccgccttg ccgcaggact gccggcgccg ctcgagctct acgccgacgc ctcggatctt
      781 gccggctcgg gcgacagtgc acgtgtgcac gccctgatcg ctcagtactc ggccctgctc
      841 ggacggatgc gcctcgccgc gcgcggcatc gatccgctgc tggtgacgac gatcgcggta
      901 gaggacatcg acgtctcgac tcccacgacc cgttccgtgc tcatcctcgg cacgctcagt
      961 tacctggtac tgctcaccat gctgatgggc ggcatgtatc tcgccatcga tgcgaccgcc
     1021 ggggagcgcg agcgcggctc gctcgagccg ctgctcaccg tgccggtgcc gcgcgagcag
     1081 ctgatctacg gcaagatcct cgccacctgc gcctacatgg tgctgtcact caccctcacc
     1141 gtgaccgcgt tcgcggtcgt gctgcgcttc accggcctcg agcgtttcgg catgagtgtg
     1201 aatttcgcac cgggggtggc gctgcagctg atcctgctca ccctgccgct ggtgccgctc
     1261 ggcgcggcgc tgatgacact ggtcgccgcc tacacccgca gctaccgcga ggcgcagacc
     1321 tatctggcgc tgctgctgct cgtgccgact ctgccgctgg ccttcgccgg gctcctgggt
     1381 ctgcggccgc acctcgccct catggcgatc ccttccctca gccagcactt cctcatcacg
     1441 agtctgctgc gcgcggaagc gttgcccgcg agctggatcg cactttccgt gggggtgacg
     1501 ctcgcggtcg ccgggctgct catcgccgtg gccggcggtc tctaccgccg cgaggcgcta
     1561 ctcggctgag tctcgctgcc cggccgcgcc gaccaggacc aggcgacgcc cgcgagcagc
     1621 gcgtgcattt gcggcgcgtt ctgatccgcg aacgcgcgcg cctcgacctc gaagcgattg
     1681 ttccagtagc cgcgcctgac acactcgagc agatagcggc gcagggtgag cgatccggtc
     1741 tgccactggc gcagcacgtg gcagtactcg tgcagcatga gccaggggtt gctgaagaag
     1801 tcggcggcgc taccgcgcag gtagatgcgc tcgactcgtg tggtcgcgca cgctccgtgc
     1861 agccgcgcga accaggagtg ctcgatcacc ttcacatgtg cgatgcgctg cccgagcagc
     1921 cgctcgagcg cgccgctgac gtcctccggg accgcgactt cgcgtccggc gcgcatatgc
     1981 actcactcct gcgtgtgcac ccaccccatg ctcggacgga agcgcagacg gcgtggtttt
     2041 ccggtggcga catcgtccgg cagcagcgac acctgctgat cctctagaaa gcgcttcacc
     2101 agcgagatca cgctgtcata gaccgagcgt gcctgcggcg tctcggcgag gtgcgccagg
     2161 atgcgcccct gcagatcgcg cgcggcggga gcgaggtcgc gcagcgactg caggcagcga
     2221 tcgagcagca gcagctcggt ggcgagggtc gtatcggtca cgtcggcggg cttggagctt
     2281 ttcacgacgc agcgcaggtg cagcagcgca cccgcctcct cggccgcatc gtacaggcac
     2341 acggcgatgg atgaggacaa ttccgtgacg aggtgcgcct cgcccgagga gacctcgaag
     2401 tggtcggcgg cgacgtgaat ttcccgactc tccatgcacg actgtatacc tgtcccccag
     2461 cgccgtcggc acggcccgct gatcaaacta tgaactgccg cacgccgcat gcgtagaata
     2521 tgccaatgcg ttccggcgga ccgcggcact ggcgtcgatt gttgctgagt gtgctcagct
     2581 gcggcgcagc ggcggccgca ctggcgcaga cgcagcagcc cccgctgtcg tcgatccgcg
     2641 tcaccggcga tgcccgcgtg accgccaagc ccgaccgggt gcagatcgac atcggtgtca
     2701 ccacccgcgc agcgttgtcg caggatgcgg cgtcgcagaa tgcgcgcgcg gtggacggcg
     2761 tgctcgctgc ggtgcgcaag gtggccgggc ccaccgcggt cctcaagacc atcagctaca
     2821 cgctcaatcc caactatcgt taccacccga aggaaggcga gccgaccatc gacggctaca
     2881 ccgcgctcaa cgtggtggag gtgacgctcg atgacctcgg ccggatcggc gcggtcatcg
     2941 atgcggcgac tcaggccgga gccaaccgca tacagggaat ccagttcacg ctgcgcgacc
     3001 aggacgccgt gcgcgcccag gccctgcgcg aggcggccac gcgcgcgcgc gccgaggtcg
     3061 acgtgctcgc tgccgcgctt ggcttgaagg tgctgcgggt gctcaccgtc gaggaaagca
     3121 gtccacgcat catgccgctg cgcgtctaca acggtgccgc gcgcgccgcg gcggcggagg
     3181 ccgcgccgac accggtggaa gccggcacgc tcgatattac tgccgacgtc acgctcagcg
     3241 tggaagtggg acccgcgtcg cgttaggcta agctggtgcg agggcgttag tgatgatcag
     3301 cgtgcgccag ttgctcgacc gcaaagggcg ggaggtattc tccgtcgagc ccggtgcggc
     3361 agtgctcgag gcgatccgcc tgatggcgga gcgtcacgtg ggtgcgctgc tcgtgatgca
     3421 gggtgataac ctcgccggca tcatttccga gcgcgactac gcccgcaagg tcatcctgat
     3481 gggccgctcc tccgccgaca cccccgtgcg cgacatcatg acggcgccgg tactcaccgt
     3541 gcagcccgag accacggtgg aaaagtgcat gcagatcatg accgaacgcc gcgtgcggca
     3601 cctgcctgtc gccgaaggcg gacgggtggt cggcatggtg tcgatcggcg acctggtgaa
     3661 ggcggtgatc gcggagcagc aacagcagat cgagcagctc gagagttata ttcatagctg
     3721 attcatagaa caagagctac agtccagtca cccaacgctc gactctggca cggatgccac
     3781 gccttatcgc tctctgctgc gccctgctgt tggctgggtg cacgacactc cccggtcgcg
     3841 actatccgcg gccaccttcg ccgttggccg ccgcgccgcc gaatcccgag ttgctgcaac
     3901 cctcctctca cgccacgcgc acccacggcg aagagtcggg tttccgcctc tactctctcg
     3961 gtatcgacgg cctgctgctg cggctcgagc tcatcgacgc cgcgcacagc tcgctcgatc
     4021 tgcagtacta cgttttccac ggcgacgagt cgggcaagct cgtcaccgag gcgctgctgc
     4081 gcgcggccgc ccgcggcgtg cgcgtgcgca tcctggtcga tgacggcgag acgatccgcg
     4141 gcgatgagca gctgtacgcg ctggccggcg agaagaacgt ggagatccgc gttttcaatc
     4201 cctggcgcta tcgcggtcac aacagcttgc tgcgcgacat cgagtatctc ttcagccacc
     4261 gccgcctcga cttccgcatg cacaacaagc tcttcatcgc ggaccgcgcg ctcgcgctcc
     4321 tgggtggccg caacatcggc gatgagtact tccaggtcga tccgagctgg cagttcgccg
     4381 acgacgacgt gctcaccacc gggcctgccg tgcagcagct ggcggtctcg ttcgaggagt
     4441 actggcagag cgagctcgct atcccggtgc aggcgctgct gccggcgaag gctaccgatg
     4501 ccgccgcggc cgcggccctg gcgcagcgcg taaccacgcc gcagaaagcg gccagcgccg
     4561 ccgctgacta cgagcagaag ctctccgcgc aggagccgct cgccagcgtg ctcgccgggc
     4621 gcgtaccgct cgattgggcc ggcgccgagg tcgccgccga cagcccctac aagggcaagg
     4681 aacacgcgca gaacgtgcac gtcagcagcc tgctgttcgc gccggtagcg caggcgatcc
     4741 acgacacgcg ttccgagctc gtgatggtca gtccgtatct ggtcccttca ccgcacgaga
     4801 tgaggctgct cgaggacctg cgtgcgcggc agcgcaccgt gcgcatcctc accagctcgc
     4861 tcgaggccga gagcagccac gcggcgcagg caggttacat gcactatcgc aaaccgctgc
     4921 tggatgccgg cgtgcagctg tacgagatcc gcgcgcgtcc ggagaacccg cgtggtaccg
     4981 gtcagtcgga gaagctctcc cgtcacgggc attacagcct gcacgccaag ctgttggtgt
     5041 tcgaccgcag cgttctgtac atcggctcga tgaactacga tcagcgctcg cgccgcctca
     5101 acaccgagga tgggctgatc attcatagcg aggatctcgc cgggcaggcc gcgcggcgct
     5161 tcgacctgtt gacgcagccg cagaacgcct atgcggtcca cttgcaggag aaagtgcccg
     5221 gcgacgctcc gcagattacc tggagcacga tcgagtccgg acggccggtg actttgtacg
     5281 atgagc
//
LOCUS       DATDMP010000353         2850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_946163, whole genome shotgun sequence.
ACCESSION   DATDMP010000353 DATDMP010000000
VERSION     DATDMP010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946163"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VI195_06205"
     CDS             complement(<1..398)
                     /locus_tag="VI195_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY6824014.1"
                     /translation="MSAIGIGALGECSPATAPYEVVERKGLGHPDTLCDALAENLSVG
                     LSRFYLERFGTILHHNVDKALLVAGSARPAFGGGELVTPIEIILAGRATRKYRGVSVP
                     VDEMAVELSRDWLRANLRHLDAERDVRIVPR"
     gene            complement(402..740)
                     /locus_tag="VI195_06210"
     CDS             complement(402..740)
                     /locus_tag="VI195_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824015.1"
                     /translation="MDSATSRARSATSRASSATRRSFSACCTALLTGVLLSGALAMMA
                     AYSGPTFACASPMLFDLRQHTAPLARAKCRGLFPKERHVNRRYVLEAHLSRRWRRIGG
                     IVIAVGIRDH"
     gene            792..1094
                     /locus_tag="VI195_06215"
     CDS             792..1094
                     /locus_tag="VI195_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824016.1"
                     /translation="MEEMRQTAEQLRQVAEEFGSTVMGSIRGEQDTWEVAERARASAE
                     EARRASEAARAAAESIRGAMAEQWKVTEEVSRAAAEMLKYAQELMHQVQRERTRTG"
     gene            complement(1125..1301)
                     /locus_tag="VI195_06220"
     CDS             complement(1125..1301)
                     /locus_tag="VI195_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824017.1"
                     /translation="MEGLPPAVRRKAIEVANALLRGGHPEGQSIRIGIASARKWAAHH
                     RALAWRPGWPDSSA"
     gene            1369..1698
                     /locus_tag="VI195_06225"
     CDS             1369..1698
                     /locus_tag="VI195_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824018.1"
                     /translation="MTHLKWLRLRDAYLGGMSQVIPQTPPDYELARLVERPDGFYWRP
                     KGTPRDFGPFATLLEAVQDMQMMDDGTPEPGETLAEAEAELGIGDWIDPETGAPAEEQ
                     RPHLEEH"
     gene            1709..2125
                     /locus_tag="VI195_06230"
     CDS             1709..2125
                     /locus_tag="VI195_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824019.1"
                     /translation="MNTRVICSMALCLAPTMASAQALVAQEQGAVIWRCGGVGVEERD
                     ELAKMEAGSNLKLVFAAGARGEYLANVQVAISERQSKVAAIKFVAEGPICLVKAPAGS
                     YRVDASVGDRKSSVSATIAEDASRPAMMVLRFPAGD"
     gene            complement(2122..2454)
                     /locus_tag="VI195_06235"
     CDS             complement(2122..2454)
                     /locus_tag="VI195_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY6824020.1"
                     /translation="MQLPLQIAWRNVAPSAPIEELIRVRAEKLQHFHPHLVSARVAVE
                     LAGRHQHKGKLFVVRLDVKVPGAEIAIDHQMHEDPHVAVRQAFDAAQRRLEDELRLQR
                     GDVKLHSR"
     gene            2560..>2850
                     /locus_tag="VI195_06240"
     CDS             2560..>2850
                     /locus_tag="VI195_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6824021.1"
                     /translation="MQIADVCNRNVVFVLARDSVADAARVMRERHVGSLVVVEGAEVR
                     RPVGMLTDRDIAVGVVALGLDPETTLVSSVMRPGLVSAHAFEPLERAVALMRD"
BASE COUNT          487 a          910 c         1014 g          439 t
ORIGIN      
        1 cgcggcacga tgcgcacgtc gcgctcggcg tcgagatgcc gcaggttggc gcgcagccag
       61 tcacggctga gctcgaccgc catctcgtcg accggcacgc tgacgccgcg gtatttccgc
      121 gtggcgcgtc cggcgaggat gatctcgatc ggcgtcacga gctcgccgcc gccgaaggcg
      181 gggcgtgcgc tgcccgccac cagcaacgcc ttgtcgacgt tgtggtgcag gatggtgccg
      241 aagcgctcga gatagaagcg cgacaggccg acgctcaggt tctccgcgag cgcgtcgcac
      301 agcgtgtcag gatggccgag gcccttcctc tcgaccacct catacggtgc agtggcgggc
      361 gagcactcgc cgagcgcgcc gatgccgatc gcgctcatgc gctagtggtc gcggatgccg
      421 accgcgatga cgatgccgcc gatgcgacgc caacgcctac tgaggtgcgc ctctagcaca
      481 tatcggcgat tcacatggcg ctccttggga aagagacccc tgcatttggc gcgcgcgagc
      541 ggtgcggtgt gttgacgcag gtcaaacaac atcgggctcg cacacgcgaa cgtcggccca
      601 ctgtaggctg ccatcatggc caacgcaccc gaaagcaaca cgcccgtcaa caacgccgtg
      661 cagcacgcgg aaaaggagcg gcgcgtcgcc gaagaagcgc gcgaggtggc cgagcgcgca
      721 cgcgaggtgg cggagtccaa tcgcgtcgag atgttgaagt cctatgaggc ggcagagggg
      781 cagcgcagat catggaagag atgcgccaga cggcggagca gcttcgccag gtggccgagg
      841 agtttggcag caccgtaatg ggcagcatcc gcggcgagca ggacacttgg gaggtcgccg
      901 aacgcgcccg cgcaagtgca gaggaagcac ggcgcgcttc cgaggcggca cgcgcggcgg
      961 ccgagtcgat acgcggggcg atggccgagc aatggaaggt gaccgaagaa gtgtcgcgcg
     1021 ccgctgccga aatgctgaag tacgcgcagg agctcatgca ccaggtccag cgcgaaagaa
     1081 cgcggaccgg ctgagcgcag ctgtggcgcg tcgctagcgc acgatcaagc gctgctgtcc
     1141 ggccagcccg gccgccaagc cagcgcgcga tggtgcgcgg cccacttcct tgcgctggcg
     1201 atgccgatgc gaatcgactg gccctcggga tggccgccac gcagaagcgc attggcgacc
     1261 tcgatcgcct tgcggcgcac ggctggcggc aggccttcca tcgaagtcgg gtagtgggct
     1321 tcgttccagg gcatggcgtc gagattgccc ctcgcccgcg atgcgccttt gacgcatctc
     1381 aaatggctgc gcttgcgcga cgcttacctt ggcggcatga gccaagtcat tccgcaaacg
     1441 ccaccggact acgagctcgc gcgcctggtc gagcgcccgg atggcttcta ctggcgtccg
     1501 aaggggacgc cacgcgattt cgggcctttc gcgacgctgc tcgaggcggt gcaggatatg
     1561 cagatgatgg acgacggcac accggagccc ggcgagacgc tggcggaggc ggaagcggag
     1621 ctcggcatcg gcgactggat cgatccggaa acgggcgcgc cggccgagga acagcggccg
     1681 cacctcgaag agcactgaag gagccccgat gaatacacgt gttatatgca gcatggcgct
     1741 ctgtctggca cccacgatgg cgagcgctca ggcactcgtc gcgcaagagc agggtgctgt
     1801 catctggcgc tgcggtggcg ttggcgttga ggagcgcgac gagctcgcca agatggaggc
     1861 cggctccaac ctgaagcttg tcttcgccgc cggggcacgc ggtgaatacc tcgccaatgt
     1921 ccaggtcgcg atctcggaac ggcaaagcaa agtcgccgcg atcaagttcg tcgcggaagg
     1981 cccgatctgc ctggtgaagg caccggcggg cagctaccgc gtggatgctt cagtcggcga
     2041 tcgcaagagc agcgtctcgg ccaccatagc cgaagacgcg agccgtccgg caatgatggt
     2101 gctgcgcttt cctgcaggcg actagcgcga gtgcagcttg acgtcgccgc gctgcaggcg
     2161 cagctcgtcc tcgagtcttc gctgcgcggc gtcgaacgcc tgacgcacgg cgacgtgcgg
     2221 atcctcgtgc atctggtggt cgatggcgat ctcggcgccg ggcactttca cgtcgagccg
     2281 gaccacgaac agcttgcctt tgtgctgatg gcgcccggcg agctccacgg ccacgcgtgc
     2341 gctgaccagg tgtggatgga agtgctgcag cttctccgcc cgaacgcgga tcagctcctc
     2401 gatgggcgcg gacggcgcca cattgcgcca ggcgatttgc agggggagtt gcataggctc
     2461 acattagcgg cgccggccgg gcgggctttg atccacgtca acggtgccca ctggcgttgc
     2521 cgcaccatgc attgactgga tccactgccg aggctcgcca tgcaaatcgc cgacgtctgc
     2581 aatcgaaatg tggtcttcgt cctcgcgcgc gactcggtcg ccgacgccgc gcgcgtcatg
     2641 cgcgagcgcc atgtcggctc gctggtggtg gtggagggcg ccgaggtgag gcggccggtg
     2701 ggcatgctca ccgatcgcga catcgccgtc ggggtggtgg cgctcgggct cgacccggag
     2761 acgacgctcg tttcgagcgt catgcggccg ggcctggtca gcgcgcatgc gtttgagccg
     2821 ctcgagcgcg cggtggcgct gatgcgcgac
//
LOCUS       DATDMP010000354         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1500971, whole genome shotgun sequence.
ACCESSION   DATDMP010000354 DATDMP010000000
VERSION     DATDMP010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500971"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1757
                     /gene="betA"
                     /locus_tag="VI195_06245"
     CDS             87..1757
                     /gene="betA"
                     /locus_tag="VI195_06245"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016977775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY6824022.1"
                     /translation="MTAETFDYVIVGAGSAGCVLADRLTESGRDSVLLLEYGGSDRSI
                     WIQMPTALNIPLNMERFDWRYYTEPEPNLNNRRLHTPRGKVLGGSSSINGLVYVRGNP
                     LDFERWESEGAAGWGYQDVLPYFRRAEAYAGGADEYRGGEGKLATRKGLLTNPLHEVW
                     LAAGREAGYPLSADMNGYQQEGFARMDMTVGGGRRCSTANAYLRPAMSRANLSVRTHA
                     LATAILFGGRRATGVRYQRNGEAQEARVRREVIVCGGSINSPQLLKLSGIGPGAELSA
                     LGIPVVQDLPGVGENLQDHLEFYFQVASREPVTLYTYMSVWRRALIGARWLLTHDGLG
                     ASNHFETGGFIRSRPGIRYPDIQFHFLPLAVSYDGSSLAREHGFQAHVGPMRSRSAGS
                     VRLRSASATDQPMIRFNYMSRPEDWLEMRACVRLTREIFGQRAFDRYRAREIQPGEHV
                     QSDEQIDDFIRAKVESAFHPSCTCKMGSRADPMAVVDSETRVIGVEGLRVVDSSIMPS
                     ITTGNLNAPTIMLAEKSADHIRGRALLPRAAAPYYVAPEWEVRQRHTA"
     gene            1754..>2074
                     /locus_tag="VI195_06250"
     CDS             1754..>2074
                     /locus_tag="VI195_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824023.1"
                     /translation="MIGWLKPAPFAAPLSTEQVDPLYRRLRRQVFVGIFAGYAAYYLV
                     RNNLALAIPDILREYPQYSKTALGSALTGLSIAYGVSKFLMGSVSDRSNPRYFLPLGL
                     LLSSA"
BASE COUNT          359 a          692 c          678 g          345 t
ORIGIN      
        1 agcactacac gcagctgaaa agcgtctacg ttgccatggc cgatatcgaa gccccgtatt
       61 gagtgcgcca tgacacacgc gccatcgtga ccgccgagac cttcgactac gtcatcgtcg
      121 gtgccgggtc tgcgggctgc gtgctcgccg accgcctcac cgaaagcgga cgggacagcg
      181 tactgctgct cgagtatggc ggctcggatc gctcgatctg gatccagatg ccgaccgcgc
      241 tcaacatccc gctcaacatg gagcgcttcg actggcgcta ttacaccgag cccgagccga
      301 acctcaacaa tcgccgcctg catactcccc gcggcaaggt gctcgggggc tcctcatcca
      361 tcaacggcct ggtatacgtg cgcggcaacc cgctcgattt cgagcgctgg gagtccgagg
      421 gtgcggccgg ctggggttat caggacgtat tgccctattt ccgccgcgcg gaagcctacg
      481 ccggcggcgc cgatgaatat cgcggcggcg aaggcaagct ggccacgcgc aagggcctgt
      541 tgaccaaccc actacacgag gtctggctcg cggccgggcg cgaagccggc taccccctca
      601 gcgcggacat gaacggctat cagcaggagg gtttcgcgcg catggacatg acggtcgggg
      661 gaggccggcg ctgcagcacc gccaacgcgt acctgcgacc cgcgatgagc cgggcgaacc
      721 ttagcgtgcg cacccacgcg ctcgcgaccg cgatactgtt cggcgggcgg cgtgcgacag
      781 gagtccgtta ccaacgcaac ggggaagccc aggaagcgcg cgtgcggcgc gaggtgatcg
      841 tctgtggagg ttcgatcaac tctccgcagc tgttgaagct atcgggaatc ggcccaggcg
      901 cggagctcag cgctctcggg atcccggtgg tgcaggacct gcccggggtg ggcgagaacc
      961 tccaggacca cctggagttc tacttccagg tggccagtcg cgagccggtc acgctctaca
     1021 cctacatgtc ggtgtggcgt cgggcactga tcggcgcgcg ctggctactc acgcacgatg
     1081 ggctcggggc gagcaaccac ttcgagaccg gcggcttcat ccgcagccgc cccggcatac
     1141 gctacccgga catccagttc cacttcctgc cgcttgcggt gagctacgac ggatcgtccc
     1201 tggcgcgcga acacggcttc caggcacatg tggggccgat gcgctcgcgc agcgcgggat
     1261 cggtgcgcct gagatccgcc tcggccaccg accagccgat gatccgcttc aactacatgt
     1321 cgcggccgga ggactggctg gagatgcgcg cctgcgtgcg tctgacgcgc gagatcttcg
     1381 ggcagcgcgc gttcgaccgc tatcgggcgc gcgagatcca gccgggcgag cacgtgcaga
     1441 gcgatgagca gatcgatgac ttcattcgcg cgaaggtcga gagcgctttt catccgagct
     1501 gcacctgcaa gatgggcagc cgcgcggacc cgatggctgt cgtcgacagc gagacgcgcg
     1561 tgatcggcgt ggagggactg cgggtagtcg actcctcgat catgccgtcc atcacgaccg
     1621 ggaatctcaa cgccccgacc atcatgctgg ccgagaagtc ggcggatcac atccgcggca
     1681 gagcactgct gccgcgcgcc gcagcacctt attacgtggc ccccgagtgg gaagtgcggc
     1741 agcggcacac ggcatgatcg gctggctcaa accggcgccc tttgcggcac cgttgtcgac
     1801 ggagcaggtg gatccgctct atcggcgtct gcgccgccag gtgttcgtcg gcatcttcgc
     1861 cggctatgcc gcctattacc tggtgcgcaa taacctcgcg ctcgcgattc ctgacatcct
     1921 cagggaatac ccgcagtaca gcaagaccgc gctcggcagc gcactgactg gactgtcgat
     1981 cgcctacggg gtgtcgaagt tcctcatggg ctcggtttcg gaccgcagca accccagata
     2041 cttcctgccg ctgggactgc tgctgtcgag tgcc
//
LOCUS       DATDMP010000355         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_391149, whole genome shotgun sequence.
ACCESSION   DATDMP010000355 DATDMP010000000
VERSION     DATDMP010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391149"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VI195_06255"
     CDS             complement(<1..433)
                     /locus_tag="VI195_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY6824024.1"
                     /translation="MSVLDRYIVRAILGTVLMVMMVLLVLGALFVFIDQQDDIGVGHY
                     TALGALWYSLLNLPQQAYELLPITALIGSLLGLGSLARGSELTVIRATGVSIARLASI
                     ALLAGLALVLFEVLLGEFLAPPLQATARAQKAFSKLSNVSFG"
     gene            complement(430..1530)
                     /gene="lptF"
                     /locus_tag="VI195_06260"
     CDS             complement(430..1530)
                     /gene="lptF"
                     /locus_tag="VI195_06260"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY6824025.1"
                     /translation="MRLGSTLGRYVLREVVVAWLAVTGVLLIILLANEVVGVMERAAA
                     NQYPQSMVLELIGLSSLQYLSIVVPVGLLLGVVLAFGRLYHDSEMAAALACGAGPRAL
                     YAPVALLAVLVAAALGWLSLVLAPEATAQALSLRNAALRAGQLAPIASGKFRSFGAGT
                     KAVLYAQNVARDGTLGEVFLERNDGPQVEVALADRATHSVTADGMTHILTLYDGERLE
                     GVPGSPEFRIVRFAEHVVTVQLPAMADAVRDLEARPTATLRHSSDRAERAELHWRVSM
                     PIMCLVLALLAVPLAKLRPRQGRYARVWVAVLIFFVYYNLATTGRTWLARGTVPEAVG
                     LWWTHAVVALLALAVILGPGIATRLRYRWRGL"
     gene            1707..>2828
                     /locus_tag="VI195_06265"
     CDS             1707..>2828
                     /locus_tag="VI195_06265"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY6824026.1"
                     /translation="MEFGVWTKGLATLSVDCLVLGVFEEARLGAQGGSVDAASGGRLR
                     KLLARGDFSGRAGETLLVAELPGITASHVLLTGMGPAKQLQRRSWRKAWSAAVSALLR
                     TRIASCAVALDRPDAKELDDYYLGRAVAELTGAALYRINDLKTARKPPPAALTKVLVG
                     PVRRAAAAERGLAHGDAVAAAQSVQRDLANLPGNICTPTYLAEQARALAKRHASLKVR
                     VLDEAAIRREKMGCLLAVAQGSHQPPRFIVLEHQGAKKLPPPIVLVGKGVTFDSGGIS
                     LKDPPGMDEMKFDMSGAAAVIAALTLAAQLRLPLHLIGLIAAVENMPGGRAIKPGDIV
                     TSASGQTVEILNTDAEGRLILSDALHYARRFQPAAVIDIA"
BASE COUNT          506 a          981 c          951 g          390 t
ORIGIN      
        1 caccgaagct gacgttgctc agcttgctga aagccttctg cgcgcgcgcg gtcgcctgca
       61 gcggcggggc gagaaactcc ccaagcagca cctcgaacag caccagcgcg agacctgcga
      121 gcagtgcgat gctcgcaagg cgcgcgatcg agacaccggt ggcccgaatt accgtgagct
      181 cgctgccgcg cgcgagcgag ccgaggccca ggagtgagcc gatgagcgcg gtgatcggca
      241 gcagctcata ggcctgctgc ggcagattga gcagcgagta ccacagcgcg ccgagcgcgg
      301 tgtaatggcc gacgccgatg tcgtcctgct ggtcgatgaa cacgaacagc gccccgagca
      361 ccagcagcac catcatcacc atcagcaccg tgccgaggat ggcgcgaacg atgtagcggt
      421 cgaggacgct cataagccgc gccaccggta acgcaggcgc gtagcgatgc ccggacccag
      481 gatcaccgcg agcgcgagca gcgcgaccac cgcatgcgtc caccacaggc ccaccgcttc
      541 cggcaccgtg ccgcgggcca gccaggtcct gccggtcgtg gcgaggttgt agtagacgaa
      601 gaagatgagc accgcgaccc acacccgcgc ataacgcccc tgccgcggcc gcagctttgc
      661 gagcggtacc gcgagcagtg ccagcaccag gcacatgatc ggcatgctga cacgccaatg
      721 cagctccgcc cgctcggccc ggtcgcttga gtggcgcagg gtcgcggtcg gccgggcctc
      781 gaggtcgcgc accgcatcgg ccatcgccgg caactgcacg gtcaccacgt gctcggcgaa
      841 gcgcacgatg cggaactcgg ggctccccgg caccccctcc aggcgctcgc cgtcatagag
      901 tgtaagaatg tgtgtcatgc catcggcggt gaccgagtgc gtggcacggt cggcgagcgc
      961 cacctccacc tgcggcccgt cattgcgctc gagaaacacc tccccgagcg tcccatcgcg
     1021 cgctacgttc tgcgcgtata gcacggcttt ggtgccggca ccgaagctgc ggaacttccc
     1081 cgaggcgatg ggcgccagct ggcccgcgcg cagcgcggca ttgcgcaggc tgagggcctg
     1141 cgcggtggcc tccggtgcca gcaccagcga aagccacccc agcgccgccg ccaccaatac
     1201 cgcgagcagc gccaccggtg cgtagagcgc tcggggccct gcgccgcagg cgagcgccgc
     1261 cgccatctcg ctgtcgtggt agagacggcc aaaggccagc accaccccga gcagcagtcc
     1321 taccggtacg acgatcgaga gatactggag ggaagagagg ccgatcagct ccagcaccat
     1381 gctctgcggg tactgattgg cggccgcgcg ctccatcacc ccgaccacct cgttggcgag
     1441 cagaatgatg agcagcaccc cggtcacggc cagccaggca accaccacct cgcgcaggac
     1501 atagcggccg agggtacttc ccagtctcat gaatgatgtg cgctgaaacc cgctgcgggc
     1561 tcttgagtta cacttgccac cacttgcacc cgtaacggcg cagtacgcgc tcaataaaac
     1621 gacacgagcg cgagacgctg taatcgccgc gaataaggta aatcagacgg ccgcgggcct
     1681 caacggcaga acttagagga caaggcatgg aattcggggt ctggacgaag ggcttggcca
     1741 cgctatcggt cgactgcctg gtgctcgggg tgttcgagga ggcgcggctc ggcgcgcagg
     1801 gcggcagcgt ggacgctgcg agcggcgggc ggctgcgcaa gctgcttgcg cgcggcgact
     1861 tctcggggcg cgccggcgaa accctgttgg ttgccgaact gcccggcatc acggcctcgc
     1921 acgtgctgct caccggcatg ggcccggcca agcagctgca gcgccggagc tggcgcaaag
     1981 cctggtcggc cgctgtgagt gcgctcctgc gcacgcgcat cgccagctgt gccgtcgcgc
     2041 tcgaccgtcc cgatgccaag gagctcgatg attactatct cggacgcgcc gtcgccgagc
     2101 tcacgggcgc cgcgctctat cgcatcaacg atctgaaaac cgcccgcaag ccgccgccag
     2161 ctgcgctgac gaaggtgctc gtaggacccg tgcgccgcgc ggcggccgcc gagcgtggtc
     2221 tggctcacgg ggatgcggtg gccgccgcgc agagcgtgca gcgcgatctc gccaatctcc
     2281 cgggcaatat ctgcaccccc acttacctcg ccgagcaggc gcgtgcgctc gccaagcgcc
     2341 acgcgagcct caaggtgcgg gtgctcgatg aggcggcgat ccggcgcgag aagatgggat
     2401 gtctgctggc agtcgcgcag ggtagccatc agccgccgcg cttcatcgtg ctcgagcatc
     2461 agggagcgaa gaagctgccg ccgccgatcg tgttggtcgg gaagggcgtc accttcgaca
     2521 gcggcggcat ctcgctcaaa gatccgccgg ggatggatga gatgaaattc gacatgagcg
     2581 gcgccgccgc ggtgatcgcg gcgctgacgc ttgccgcgca gctgcgcctg ccgctgcacc
     2641 tcatcggcct catcgccgcg gtggagaaca tgcctggcgg ccgtgcgata aagcccggcg
     2701 atatcgtcac cagcgcctcg gggcagacgg tggagatcct caacaccgac gcggagggac
     2761 ggctgatcct gagcgatgcc ctgcactacg cccgccgctt ccagccggcg gcggtgatcg
     2821 acatcgcg
//
LOCUS       DATDMP010000356         2659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_57951, whole genome shotgun sequence.
ACCESSION   DATDMP010000356 DATDMP010000000
VERSION     DATDMP010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57951"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1155)
                     /gene="purM"
                     /locus_tag="VI195_06270"
     CDS             complement(124..1155)
                     /gene="purM"
                     /locus_tag="VI195_06270"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY6824027.1"
                     /translation="MTEGPVITYRDAGVDIDAGDELVELIKPLVRRAQRREVLAGIGG
                     FGALIELPSGYRQPVLVAGTDGVGTKLRLAIDTGRHDTIGIDLVAMCANDVVVQGAEP
                     LFFLDYYATGKLQVAIAEAVVRGIVEGCVQAGAALVGGETAEMPGIYHDADYDLAGFC
                     VGVVEKAAIIDGSAVAAGDTIIGLDSSGPHSNGYSLIRKLVAVSGATATTMLAGRALF
                     ERLLEPTRIYVKPLLALLRALPVHGLAHITGGGLSDNIPRALPPGLEAVLERRRWGSD
                     PVFEWLQRTSRIDSAQMYRTFNCGIGMAVIVPAVHAAAAVALLTAQGESAQIIGEVRS
                     GDRGAVIQE"
     gene            1250..2227
                     /locus_tag="VI195_06275"
     CDS             1250..2227
                     /locus_tag="VI195_06275"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEY6824028.1"
                     /translation="MSSPASTSNPATAYLRGVLALACLLCCALPCEAGRPVRVYEVDI
                     EGQTPAALQDAMRQVLVRATGRRESADDPALASLISDAPRYVKSYSTGPRAEPQVVFD
                     AAAVEHAIGAAGRSVWERERPFTLVVLDPPRTRASEDAARTELERVAAERGLPISLIP
                     LPLTDGAGNALGADALLQSAQRYGGDQILVGRGADAGPEAPLQWTLYTRASTTSWSGP
                     LAAGVEHTVDVLVPQPATSLAEADAPARVQIEGVNTLLGYATVERLLQSTPGVRRANI
                     AAADAASVTFDVIARGGAAGLGQALAGSTRLVRVGGGGDPVVYRYQPQG"
     gene            2251..>2659
                     /locus_tag="VI195_06280"
     CDS             2251..>2659
                     /locus_tag="VI195_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY6824029.1"
                     /translation="MRHLPNLICLVRLALIWPILSALQAGGYRTALALFVLAAASDGL
                     DGYLAKRFNWTSELGKFLDPLADKLLIVLVFIEAAWLELVPWWLTAAVVARDVMIGLG
                     ALIFRLWFGPLHGRPTRFSKVNTGAQLLYVTFAM"
BASE COUNT          393 a          935 c          909 g          422 t
ORIGIN      
        1 gatcggagat caccatgacg ggctgcgccg cgatctcgcc cgcgagcgcc gcgtgcagga
       61 tggcgctcat gttggagccc cggccggaga tgaggagcgc gagcttcagc ggcgtgcgtg
      121 cgctcattcc tggatgactg cgccgcggtc gccgctgcgg acctcgccga tgatctgcgc
      181 gctctcgccc tgtgcggtca gcagcgctac tgcagccgcg gcatgcaccg ccggcacgat
      241 cacggccatg ccgatgccgc aattgaaggt gcggtacatc tgcgccgagt cgatgcgcga
      301 ggtcctctgc aaccactcga acacagggtc gctgccccag cggcgccgtt cgagtaccgc
      361 ctcgagcccg ggaggcagcg cgcgtggaat gttgtcgctc aggccgccgc cggtaatgtg
      421 ggcgagtccg tgcacgggca atgcacgcag cagcgcgagc agcggcttca cgtaaatgcg
      481 ggtcggctcg agcagccgct cgaatagcgc gcgccctgcc agcatggtgg tggcggtcgc
      541 gccgctcacc gcaaccagct tgcggatcag cgagtagccg ttcgaatgcg gcccggaaga
      601 gtcgagcccg atgatggtgt ctccggcggc gactgcgctg ccgtcgatga tggccgcttt
      661 ctccaccacc ccgacgcaaa agcctgccag gtcgtagtca gcgtcgtgat agatccccgg
      721 catctcggcc gtctcgccac cgaccagtgc ggcacctgcc tgcacacagc cctcgacgat
      781 gccgcgcacc accgcctcgg cgatcgccac ctgcaacttg ccggtggcgt agtagtcgag
      841 aaagaacaac ggctcggcac cctgcaccac cacatcgttg gcgcacatgg cgaccaggtc
      901 gatgccgatg gtgtcgtgac ggccggtgtc gatcgcgagg cgcagcttcg tgcccacgcc
      961 atcggtgccc gcgaccagca ccggctggcg atagcccgag ggcaactcga tcagtgcgcc
     1021 gaaaccgcca atgcccgcga gcacctcgcg ccgctgcgca cggcgtacca gcggtttgat
     1081 gagctcaacg agctcatcgc ccgcgtcgat gtcaacgccg gcatcgcgat aggtgatgac
     1141 ggggccttct gtcatggggc aaagcgtgct gcaggaccgg tggccgggga cgcgggcgcg
     1201 tatgctattg tacccgccgc ttcgcgccct tgcgcgtgcg cggagaagca tgtcctcacc
     1261 ggcctcaaca tccaatcccg cgacggcata cctgcgcgga gtgcttgcgc tggcgtgcct
     1321 gctgtgctgc gcactgccgt gcgaggccgg tcggccggtg cgtgtctacg aggtcgatat
     1381 cgaaggacag acgcccgcgg cgctgcagga cgccatgcgc caggtattgg tgcgtgccac
     1441 cggccggcgc gaatccgccg acgatccggc gctcgcgagc ctgatcagtg atgcgccgcg
     1501 ctacgtgaag agctacagca cggggccgcg cgctgagcca caggtggtgt tcgatgccgc
     1561 agcggtcgag cacgccatcg gtgccgcggg ccggagcgtg tgggagcgcg agcgtccatt
     1621 cacgctcgta gtactcgacc cgccgcgcac gcgcgccagc gaggatgccg cacgtaccga
     1681 gctcgagcgt gtggccgccg agcgcgggct gccgatcagc ctcattccgt tgccgctcac
     1741 cgatggggcc ggcaacgcgc tcggcgcgga tgcgctgctg cagtccgcgc agcgctatgg
     1801 cggcgatcag attctcgtgg ggcgcggtgc ggatgcgggt cccgaggcgc cgctgcagtg
     1861 gacgctctac acccgtgcca gcaccaccag ctggagcggg ccgctcgcgg ccggcgtcga
     1921 gcacaccgtc gatgtgctcg tgccgcagcc ggcaacttcg ctcgcggagg cggacgcgcc
     1981 ggcgcgtgtg cagatcgagg gcgtcaacac gctcctcggc tatgcgacgg tcgagcgcct
     2041 gctgcagagc acccccggcg tacgccgcgc caacatcgcc gccgccgatg ccgccagcgt
     2101 caccttcgat gtcatcgcac gcggcggcgc ggccgggctc ggccaggcac tcgcgggctc
     2161 cacgcggctc gtgcgcgtgg gcggcggggg cgacccggtg gtgtaccgct accaacccca
     2221 gggctgaaac gcctgcggct ccgcaaggtc gtgcgccatc tgcccaacct catctgcctc
     2281 gtgcgcctcg cgctgatctg gccgatcctc agcgcgctgc aggcgggcgg gtaccgcacg
     2341 gcgcttgcgc tcttcgtcct ggctgccgct tccgacgggt tggatggcta tctcgccaag
     2401 cgcttcaact ggacctccga gctcggcaag ttcctcgacc cgctcgccga caagctgctc
     2461 attgtcttgg tgttcatcga ggcggcatgg ctcgaactgg tgccgtggtg gctgaccgcc
     2521 gcggtcgtcg cgcgcgatgt gatgatcggc ctcggtgcgc tcattttccg cctctggttc
     2581 ggcccgctgc acggccggcc gacacgcttc agcaaagtca acaccggggc acagctgctc
     2641 tacgtgacct tcgcgatgc
//
LOCUS       DATDMP010000357         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_169130, whole genome shotgun sequence.
ACCESSION   DATDMP010000357 DATDMP010000000
VERSION     DATDMP010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169130"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VI195_06285"
     CDS             <1..368
                     /locus_tag="VI195_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEY6824030.1"
                     /translation="GSGTLQLRALAANAERVFLPGLFARVRIARGAPAARLMVPDRVV
                     GNDQVGSYVMLVAADHKVQQRRVEVGPLQDGLRAIVSGLEPDSKVVVDGLQNAVPGNT
                     VTPNERPLTAPAVAAAATR"
     gene            384..>2690
                     /locus_tag="VI195_06290"
     CDS             384..>2690
                     /locus_tag="VI195_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6824031.1"
                     /translation="MLAKFFIERPVLANVIALLIMLLGFVAIFALPVAQYPPITPPTV
                     QVTTRYPGANARTLINTVALPIEQQVNGVQNMLYMQSTSTNDGNYTLTVTFKVGTDLD
                     FAQVLVQNRVAAAIASLPDAVQSNGVVTKKKSTAILQIITLTSSEPRYDSLFLSNFAA
                     IRMRDVLARLPGVGDVNIFGIGQYAMRVWLNPQQLLARSLTPGDVINSIRQQSQEVAA
                     GQIGTPPVSAGQDFQLTVNVPGAFSKVEEFEDIIVKTESAAGGSRAITRLRDIGRVEL
                     GAQTYSQFFTMDGKPAAGIAIFQLPEANALDVAQLVKAKLSEMSRDFPKGLEYSIPFD
                     TTRFVSASIHEVYRTLFEAGILVLIVIVVFLQNWRATLVPATTVPVTIIGAFAAMAAM
                     GFSINTLTLLSLVLAIGIVVDDAIVVVEGVSRHIEQGESPHDGAIAAMRELMGPIMGI
                     TLVLMAVFLPAAFMPGITGQMYRQFALVIAATAVISAINAVTLKPTQCALWLRQVDPR
                     ATKNWFFRGFDAVYAPVERAYTRLIARMVARSGLMVVIATALIVCAGFALSRIPTSFI
                     PLEDQGYLLLAAQLPDAASLERTQEVLKRMSATALQLPGVEHAISVGGVSVLDNSASL
                     ANAGVVYLTLRDWDERYKAKGQDLRSLYRKLTAAMAQIEDAHVLVVPPPPIQGIGNSG
                     GFQLQVELTDGSFDYRKLQRVSDLLVSHANTQSGLEHVMTTFRAEVPQLAADINRAKA
                     QTLNVSVGNVFSTLQSYLGSTYTSQITKF"
BASE COUNT          450 a          915 c          865 g          460 t
ORIGIN      
        1 ccggcagcgg cacgctgcag ctgcgcgcgc tggccgccaa tgccgagcgg gtattcctgc
       61 cggggctgtt cgcgcgcgta cgcatcgcgc gcggtgcacc ggccgcgcgg ctcatggtcc
      121 ccgaccgcgt ggtgggcaac gatcaggtcg gcagctacgt gatgctggtc gcagccgatc
      181 acaaggtgca gcagcgccgc gtcgaggtcg ggccgctgca ggatggtctg cgcgccatcg
      241 tctccgggct cgagcccgac agcaaggtgg tcgtcgatgg gctgcagaat gccgtgcccg
      301 gcaacaccgt gacccctaac gagcgcccgc tcacggctcc ggcggtcgcc gcggcggcca
      361 ctcgctgagg cgcagctcgc accatgctcg cgaaattctt catcgagcgg ccggttctcg
      421 ccaacgtcat cgcgctgctc atcatgctgc tcgggttcgt ggcgatcttc gcgctgccgg
      481 tggcgcaata tccgccgatc accccgccca ccgtgcaggt gaccacgcgt tatccgggcg
      541 ccaatgcccg cacgctcatc aacaccgtgg cgctgcccat cgagcagcag gtgaacggcg
      601 tgcagaacat gctgtacatg cagtcgacca gcaccaacga cggcaattac acgctgacgg
      661 tgaccttcaa ggtgggcacc gatctcgatt ttgcgcaggt gctggtgcag aaccgcgtcg
      721 ccgcggcgat cgcctcgctc ccggatgcgg tgcagagcaa cggtgtggtc accaagaaga
      781 aatccaccgc catcctgcag atcatcaccc tcaccagcag cgagccgcgc tacgacagcc
      841 tgttcctcag caatttcgcg gccatccgca tgcgcgatgt gctcgcgcgg ctgccgggcg
      901 tcggagacgt caacatcttc ggcatcggcc agtacgccat gcgggtgtgg ctgaatccgc
      961 agcagctgct ggcacgctcg ctcacgcccg gggatgtcat caactcgatc cggcagcaga
     1021 gccaggaagt ggccgccggg cagatcggca ctccgccggt gagcgcgggc caggactttc
     1081 agctcaccgt caacgtcccc ggggcattca gcaaggtcga agagttcgag gacatcatcg
     1141 tcaagaccga gtccgccgcc ggtggcagcc gtgcgatcac ccgcttgcgc gatatcggac
     1201 gcgtcgagct cggcgcccag acctacagcc agttcttcac catggacggc aagccggcgg
     1261 cgggcattgc catcttccag ctgccggagg cgaacgcgct cgatgtcgcg cagctggtga
     1321 aggcgaagct cagcgagatg tcgcgcgatt tccccaaggg gctcgagtac agcattccct
     1381 tcgacaccac gcgtttcgtc tccgcctcga tacacgaggt gtaccgcacg ctgttcgagg
     1441 ccggcatcct ggtgctgatc gtgatcgtgg tgttcctgca gaactggcgt gcaacgctgg
     1501 tgccggccac taccgtgccg gtcacgatca tcggcgcctt cgccgcgatg gccgcgatgg
     1561 gcttctcgat caacacgctg acgctgctgt cactcgtgct ggcgatcggc atcgtggtgg
     1621 acgacgccat cgtggtggtc gaaggggtga gccgtcacat cgagcagggc gagtcgccgc
     1681 acgatggcgc catcgcggcg atgcgcgagc tcatgggtcc gatcatgggc atcacgctgg
     1741 tgctcatggc ggtgttcctg ccggcggcgt tcatgcccgg aatcaccggc cagatgtacc
     1801 ggcagttcgc actggtgatc gccgctaccg cggtaatcag cgccatcaac gccgtcacac
     1861 tcaaaccgac gcagtgcgca ttgtggctgc gccaggtcga tccgcgcgcg accaagaact
     1921 ggttcttccg cggtttcgat gcggtgtatg cgccggtcga gcgggcctat acccgactca
     1981 tcgcgcgcat ggtggcgcgc agcggcctca tggtcgtcat cgcgaccgcg ctcatcgtct
     2041 gtgcgggctt cgccctgtcg cgtattccga cgagcttcat cccgctcgag gatcaggggt
     2101 atctgttgct cgccgcgcag ctgcccgatg cggcctcgct cgagcgcacc caggaggtgc
     2161 tgaagcgcat gagcgcgacc gccctgcagc tgccgggcgt ggagcacgcc atctcggtcg
     2221 gcggagtctc ggtactcgac aacagtgcct cacttgccaa cgccggcgtg gtctatctca
     2281 cgctgcgcga ctgggacgag cgctacaagg cgaagggcca ggacctgcgc tcgctgtacc
     2341 gcaagctgac tgcggcgatg gcgcagatcg aggacgcgca cgtgctggtg gtgccgccac
     2401 cgcccattca gggcatcggc aactctggcg gctttcagct gcaggtcgag ctcaccgacg
     2461 gcagcttcga ttaccgcaag ctgcagcgcg tgtcggatct gctggtctcc catgccaaca
     2521 cgcagagcgg gctggagcac gtgatgacga ccttccgcgc ggaggtgccg cagctcgccg
     2581 ccgacatcaa ccgcgccaag gcgcaaacgc tcaacgtatc cgtgggcaac gtcttcagca
     2641 cgctgcagag ctatctgggc tccacctaca ccagccagat caccaagttc
//
LOCUS       DATDMP010000358         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1946496, whole genome shotgun sequence.
ACCESSION   DATDMP010000358 DATDMP010000000
VERSION     DATDMP010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946496"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /gene="aroE"
                     /locus_tag="VI195_06295"
     CDS             <1..683
                     /gene="aroE"
                     /locus_tag="VI195_06295"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY6824032.1"
                     /translation="RFFEKGGRGLNITMPHKLAAVEVVQELTARAAHAAAVNTLVRHD
                     DGILGDNTDGVGLVHDLCDNLGLIITQRRLLILGAGGATRGVLAPLLGLEPAIVMIAN
                     RTPERAEALAAAFADLGVTQGVGFSYVGDTPFDLIINATSASFSGEIPPIPAGAIGPQ
                     TVCYDLAYGRSAMAFVRWAQQSGCAMAAQGLGMLVEQAAESFRLWRGVRPQTKEVLAA
                     LKERVGSV"
     gene            complement(709..2088)
                     /gene="gor"
                     /locus_tag="VI195_06300"
     CDS             complement(709..2088)
                     /gene="gor"
                     /locus_tag="VI195_06300"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF004776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY6824033.1"
                     /translation="MSAGVSEGAMAEKYDLVVIGGGSGGLAAAQRAAEYGAKVVLAES
                     GRLGGTCVNVGCVPKKIMWNAAELGSALQDARDYGFGVESAGVDWPGLKAKRDAYIER
                     LNEMYAANLAKRDIELVRARASFHDAHTVSAGQRLLRADHIYIATGGRPRLPLIPGAQ
                     LGITSDDFFDLPKRPERVAVVGSSYIAIELAGIFAGLGTQTALVLRGDTALKSFDAML
                     GESALIMLRDDGVEIVLNAIPVELRRTTHAALELRCLDGRILGPFDCVLWAIGRGAEV
                     ETLALERAGVGLDVQGFVANDRYQVTNVPGIYAIGDVSGRAQLTPVAIAAGRRLSDRL
                     FGGQAGRRLDYENIPTVIFGRPPLGTVGLSEKAAAERYGADKLTVYRSSFVPLYHALT
                     SAKPRSQLKLITAGSEQRIVGLHVAGQGADEMLQGFAVAVRMGATKQDFDDTVAVHPT
                     SAEEFVTLR"
BASE COUNT          349 a          788 c          738 g          355 t
ORIGIN      
        1 agcgcttttt cgaaaagggc ggccgcgggc tgaacatcac catgccccac aagctcgcgg
       61 ccgtggaggt ggtgcaggag ctgactgcgc gcgccgcgca cgccgcggcg gtcaacacgc
      121 tcgtacggca tgacgatggc atcctcggcg acaacaccga cggggtgggt ctggtgcacg
      181 atctgtgcga caacctcggg ctcatcatca cgcagcgccg gctgctgatc ctcggcgcag
      241 gcggcgcgac tcgcggcgtg ctcgccccgc tgctcggtct cgagcctgcg atcgtaatga
      301 tcgccaaccg cacgcccgag cgggccgagg cactcgccgc cgcctttgcc gacctcggtg
      361 tgacgcaggg agtcggcttt tcctacgtcg gcgacacgcc cttcgacctc atcatcaatg
      421 cgacctcggc aagcttctcg ggggagatac cgcccattcc tgcgggcgcc atcggcccgc
      481 agacggtgtg ctacgacctc gcctacggac gctcggcgat ggccttcgtg cgctgggcgc
      541 agcagtccgg ctgcgccatg gcggcgcagg gcctcggaat gctggtcgag caggcggcgg
      601 agtccttccg gctgtggcgc ggcgtgcggc cgcagaccaa ggaagtgctc gcggcgctga
      661 aggagcgtgt cggcagcgtc taggcacccc accctggggc cggcggagtc agcgcagggt
      721 gacgaattcc tccgccgagg tcggatggac cgccaccgtg tcatcgaagt cctgcttggt
      781 cgcacccata cgcaccgcga ccgcgaagcc ctgcagcatc tcatcggctc cctggccggc
      841 gacgtgcagg ccgacgatgc gctgttcgga tcccgcggtg atgagcttca gctgcgagcg
      901 cggcttggcg ctggtcaggg cgtggtacag cggcacgaag ctcgagcggt acacggtcag
      961 cttgtccgcg ccgtagcgtt cggcggccgc cttctccgat agccccacgg tgccgagcgg
     1021 cgggcggccg aagatcaccg tggggatgtt ctcgtagtcg agacgccggc cggcctgacc
     1081 gccgaacagc cggtccgaga gacgccggcc cgcggcgatc gcgaccgggg tcagctgcgc
     1141 gcgcccggag acatcgccga tggcatagat gcccggcacg ttggtcacct ggtacctgtc
     1201 gttggcgacg aatccctgga cgtcgagccc cacccccgca cgctcgagcg caagagtctc
     1261 cacctccgca ccgcgcccga tcgcccagag cacgcaatcg aaggggccga ggatgcgacc
     1321 atcgagacag cgcagctcga gcgccgcgtg cgtggtccgg cgcagctcca ccggtatcgc
     1381 attgaggacg atctccacgc catcatcccg cagcatgatg agtgccgact ccccgagcat
     1441 ggcatcgaag gatttgagcg cggtgtcgcc gcgcagcacc agcgccgtct gcgtgccgag
     1501 accggcgaag atcccggcaa gctctatggc gatgtagctg ctgccgacca ccgcgacccg
     1561 ttccgggcgc ttgggcaggt cgaagaagtc gtccgaggtg atgccgagct gtgcgcccgg
     1621 tatcagcggc agccgcggcc ggcctccggt cgcgatgtag atgtgatcgg cgcgcagcag
     1681 acgctggccg gcgcttaccg tatgcgcatc gtggaagctg gcgcgggcgc gcaccagctc
     1741 gatgtcgcgc ttggcgaggt tggccgcgta catctcgttc agccgctcga tgtaggcatc
     1801 acgtttggct ttgagcccgg gccagtcgac acccgcgctc tcgaccccga aaccgtaatc
     1861 gcgcgcatcc tgcagtgcgc tgccgagctc ggcggcgttc cacatgatct tcttcggcac
     1921 gcagccgacg ttcacgcacg tgccaccgag cctgcctgac tcggcaagca ccaccttggc
     1981 cccgtactcc gcggcgcgct gtgcggcggc gagtccgccg ctccccccgc cgatcaccac
     2041 caggtcgtac ttttccgcca tggcaccctc gcttaccccg gcgctcatca tgttatccaa
     2101 gacgcgcagc ggatatgagg gccgctacaa tggccgccgg cgatgggacg agacgcgtgc
     2161 atggacaatc ccttacttca ttcggactct ctgccccaat tcatgcgcat ccgccccgag
     2221 cacgtcgagc
//
LOCUS       DATDMP010000359         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1724934, whole genome shotgun sequence.
ACCESSION   DATDMP010000359 DATDMP010000000
VERSION     DATDMP010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724934"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VI195_06305"
     CDS             <1..333
                     /locus_tag="VI195_06305"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY6824034.1"
                     /translation="WAEILKPLEFSDDDLAVDAIKDTAAGGHFFGAAHTLARYQSAFY
                     RPLLSDWSSFESWTEAGARNATERATAIWKKLLAEYVPPPLDPALREAIADYVTRRAR
                     ELGQAEPI"
     gene            343..1158
                     /locus_tag="VI195_06310"
     CDS             343..1158
                     /locus_tag="VI195_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY6824035.1"
                     /translation="MSPWVMNARMYAVTPGVETTWRTLLEHIARDAGVTLTYLPYPAP
                     QPLEALWSRPDLGAVFMCGYPIALQLAPVVPLAAPIPRATWAAGRAVYRSDLIVREDA
                     PYRSLEDTFGGRAGWTVSHSQSGFNAFRHHLLAYRTAQRPALYREMVGNLVTARNLLD
                     SVRAGSIDVGPLDAYWHLLIARHAPELTHGVRVLTSTELAPMPAFVAAAGAPSELIGQ
                     LRGAFLGARGRGWFGPLAERLLLEGFAEVSVESFEPLLRLERAAKAAGFDRPA"
     gene            complement(1163..>2186)
                     /locus_tag="VI195_06315"
     CDS             complement(1163..>2186)
                     /locus_tag="VI195_06315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6824036.1"
                     /translation="NHSGMSIGTPPERDLPGAPVIRDSPRVSVEIPTLLLILADYLSW
                     LAITFAYTRWPLAVVAPITVVVITLHSSLQHESVHGHPTRWERLNRLFAMVPLSLWLP
                     YERYHHTHRAHHINPRLTDPLDDPESYYWRPEDLARLNPVSRFLHEVQQTLAGRIVLG
                     SFWRIAMFLRDEAHALLHIEKRALVRNRHNVRRVWLEHLVWCVPVILWLKLVCGMPLW
                     IYFLAMVVPANGLLLIRAFAEHRWSPGVRERIGLVEDSWILGPLFLFNNLHSLHHQEP
                     SIPWYQYNARYRVLRERLILENGGLVYSTYFEVARRYLFRRHDVLPHPVGRVPTAAAR
                     EIPDPA"
BASE COUNT          374 a          707 c          743 g          362 t
ORIGIN      
        1 tgggcggaga tcctcaagcc gctcgagttc agcgacgatg acctcgcggt cgatgctatc
       61 aaggacaccg ccgcgggcgg ccatttcttc ggcgccgcac acacgctcgc gcgctatcag
      121 tccgcgttct atcgcccgtt gctctcggac tggtcgagtt tcgagagctg gaccgaggcg
      181 ggggcgcgca acgccaccga gcgcgcaacg gcgatctgga agaagctgct ggcggagtac
      241 gtgcctccgc cactcgatcc ggcgctgcgc gaggcgattg ccgattacgt gacgcgccgg
      301 gcgcgcgagc tgggccaggc ggaaccgatt tgaggtgagc aggtgagccc ctgggtgatg
      361 aatgcccgca tgtacgcggt cactccgggc gtcgagacaa cctggcgcac gctcctcgag
      421 cacatcgccc gcgatgcggg cgtgactctc acctacctcc cctatccggc gccgcaaccg
      481 ctcgaggcgt tgtggtcgcg gccggatctc ggagcggtct tcatgtgcgg ctacccgatc
      541 gccctgcagc tcgcgccggt cgtcccgctc gccgcgccga ttccgcgcgc tacctgggcg
      601 gcaggccgcg cggtgtatcg cagcgacctg atcgtgcgcg aggacgcgcc ctaccgcagc
      661 ctcgaggaca ctttcggcgg gcgcgccgga tggaccgtca gccactcgca gtcgggtttc
      721 aacgccttcc gtcaccacct gctcgcctac cgcactgcgc aacgcccggc gctctaccgg
      781 gagatggtcg gcaacctcgt gaccgcacgc aacctgctcg acagcgttcg tgccggaagc
      841 atcgatgtcg ggccgctcga tgcctactgg cacctgctga tcgcccgaca cgcgccggag
      901 ctcacccacg gcgtacgcgt gctgacctcg accgagttag cgccgatgcc ggcattcgtc
      961 gccgcggccg gcgccccatc cgagctgatc ggccagctgc gcggggcctt cctcggcgcc
     1021 cgcgggcgcg gctggttcgg tccgctggcc gagcggctgt tgctcgaggg ctttgccgag
     1081 gtgagcgtgg agtcgttcga gccgctgcta cgcttggagc gcgcagcaaa ggctgccggc
     1141 ttcgatcggc cggcctgacc gcctacgcgg ggtccgggat ttcgcgtgcg gccgccgtcg
     1201 gtacgcggcc cacaggatgc ggcaatacgt cgtggcggcg aaacagatat cgacgcgcca
     1261 cctcgaagta ggtggagtag accagcccgc cgttctccag gatcagccgc tcgcgcagca
     1321 cgcggtagcg ggcgttgtac tggtaccagg gaatgctggg ctcctggtgg tgcagcgaat
     1381 gcagattgtt gaacaggaac aagggtccca ggatccatga atcctcgacc aggccgatgc
     1441 gctcgcgcac gcccgggctc cagcgatgct cggcaaatgc ccggatcagc aggagaccgt
     1501 tggcggggac caccatagct aggaagtaga tccatagtgg catgccacac acgagcttca
     1561 accacaggat caccggtacg caccatacga ggtgctcgag ccacacccgg cgcacgttat
     1621 gtcggttacg gaccagcgcg cgcttttcga tgtgcagcag tgcgtgtgct tcatcgcgca
     1681 gaaacatggc gatgcgccag aaggaaccga gcacgatacg gccggcgagt gtctgttgga
     1741 cctcatgcag aaagcggctt acgggattga gccgcgccag atcctccggc ctccagtagt
     1801 aagactcggg gtcgtcgagg gggtcggtga ggcgcgggtt gatatggtgt gcccggtgag
     1861 tatggtggta ccgctcgtaa ggcaaccata acgacaaggg caccatggcg aagagccggt
     1921 tgagacgttc ccaacgcgtc gggtggccgt gcacgctctc atgctgcagg gagctgtgca
     1981 gcgtgatgac aacaacggtg atgggcgcca cgaccgccag tggccagcgt gtataggcaa
     2041 aggtgattgc cagccagctg aggtagtcgg ccagtatgag aagtagagtc gggatctcca
     2101 ccgagacgcg cggactgtcg cgaatcacgg gcgccccggg aaggtccctc tcgggcggag
     2161 ttccgataga cattccggag tgattc
//
LOCUS       DATDMP010000360         3605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1280841, whole genome shotgun sequence.
ACCESSION   DATDMP010000360 DATDMP010000000
VERSION     DATDMP010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280841"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2800
                     /locus_tag="VI195_06320"
     CDS             <1..2800
                     /locus_tag="VI195_06320"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824037.1"
                     /translation="DFAVESLQGDIFQLGNTAYRIMRVERGTVRVEDAQGQPPTIPFW
                     LGEAPSRSEELSHSVSRFRTELERRFEDRSNDALEWMSSDLGLDRAASEQIVDYLGAA
                     YRALGALPTCETLIVERFFDEAGGMQLVIHSPYGSRINKAWGLALRKRFCRTFNFELQ
                     AAATEDNIVLSLTTAHSFDLAEVARYLNSKTVREILIQALLDAPMFGTRWRWVAGVAL
                     ALPRFRGGKKVPPQLMRMMAEDLVGTIFPDQVACAENLVGEREVPDHPLVAQTISDCL
                     NEAMDIDGLERLLGQIESGAIRIVARDLTEPSPLAREVLTARPYAYLDDAPLEERRTQ
                     AVMSRRWLDPDSADGIGRLDAAAVTRVREEAWPDAGNEDELHDALVWLGFVTEAEADA
                     RENWKDWLLALAQSRRVALLTAQEGVFWVPAERLRQFEAVYRCAVVEPPIAPPASADR
                     AWEPDAALAELVRGRLEGQGPLTLAELAATFGLQPERISTALTSLEVEGFALAGSFTP
                     GGSEREWCERRLLARVHRYTVKRLRAEIEPVSARDYLRFLFDWQGVSPDGRREGAQAL
                     DAVVEQLAGFEAPAVAWEGSILPARLSHYEPSLLDDACLAGRIAWARIGAPAPKSKSN
                     GRRSVPLKSTPISLFPRKAAGVWPPADEAPQVSARAAKVAAFIAENGASFFDEIVDGT
                     HLLRTQIEEALAELVAAGQVISDSFGGLRALLTPARHHKRRPNGARLAGAGRWALAKR
                     KAANGAAKTDDVERVEEIALALLRRYGVVFMRMLEREASRLPKWRVLLRVYRKLEARG
                     DIRGGRFVAGFSGEQFALPEAVAALRSIRRRAPDDSLISVSGADPLNFAGILTPGAKL
                     AGLAGNRVLYRDGIPIAFLEGDATRLLEPVEPEIEAMARAALHGHAKPAAHLPRLRRL
                     AELDQARRHRGRAGPFAA"
     gene            3100..>3605
                     /locus_tag="VI195_06325"
     CDS             3100..>3605
                     /locus_tag="VI195_06325"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY6824038.1"
                     /translation="MSKADHPQRPDPVEIRSCGGDARQFSPSAARNCGPIREVLIRVL
                     PKKGIALEIGSGTGEHVIAFAKALPGLVWLPSDPDAASRASIKAWRATEGLTNVQAPV
                     AIDVRERVWGVEDAAPFDAMISLNMVHIAPWESALGLLAGAGRLLRPDGVLVFYGPFM
                     LGGIHTAAS"
BASE COUNT          566 a         1156 c         1218 g          665 t
ORIGIN      
        1 ggacttcgcc gtcgagagcc tccagggcga tatcttccag ctcggcaaca ccgcctatcg
       61 catcatgcgc gtagagcgcg gcacggtgcg tgtcgaggac gcgcaaggcc agccgccgac
      121 gatcccgttc tggctcggcg aggcgccgtc ccgcagcgag gagttgtcgc actcggtatc
      181 tcgttttcga accgagctcg aacgccgctt tgaggaccgc agcaatgatg cgctggagtg
      241 gatgagcagc gatctcggtc tcgaccgcgc ggcatccgag cagatcgtcg attacctcgg
      301 cgcggcctac cgcgcgctcg gcgctcttcc cacttgcgaa acgctcatcg tcgagcgttt
      361 cttcgacgag gcgggtggca tgcagctggt gatccattct ccttatggca gccgcatcaa
      421 caaggcctgg gggctcgcgc tccgcaagcg cttctgccgc acgttcaatt tcgagttgca
      481 ggcggcggcg acggaagaca acatcgttct atcgctgacc accgcgcaca gtttcgacct
      541 cgccgaggtg gcgcgctatc tcaactccaa gacggtgcgt gaaattctca tccaggcgct
      601 gctcgatgcg ccgatgttcg gcacgcgctg gcgctgggtc gcgggcgtcg cgctcgcgct
      661 gccgcgcttc cgcggcggca agaaagtgcc gccgcagctc atgcgcatga tggccgagga
      721 cctggtcggc acgatcttcc ccgaccaagt ggcgtgtgcc gagaatttgg tcggcgagcg
      781 cgaagtgcct gaccatccgc tcgtggcaca aacgatttca gactgcctca acgaggcgat
      841 ggatattgat gggctggagc gtctacttgg ccagatcgag tccggtgcga tccgcatcgt
      901 cgcgcgcgac ctcaccgagc cctcgccgct cgcgcgcgag gtgctgactg cgcggcctta
      961 cgcctacctc gacgatgcgc cgctcgagga gcggcgcacg caagcagtga tgagccgacg
     1021 ctggctcgat cccgactccg ccgacgggat cggccgtctc gacgcggccg cggtgacgcg
     1081 agtgcgcgag gaggcttggc ccgacgcggg caatgaagac gagctgcatg atgcgctggt
     1141 gtggctcggg ttcgtgaccg aggcggaggc cgatgcgcgc gagaattgga aggactggct
     1201 ccttgcgctg gcgcagtcgc gccgtgtcgc gctactcacg gcgcaagaag gcgtgttctg
     1261 ggtgccggcc gagcggctac gtcagttcga ggcggtttat cgctgtgccg ttgtcgagcc
     1321 ccccatcgcc cctccggcga gcgcggatcg ggcttgggag cctgatgcgg ccctggcgga
     1381 gctcgtgcgc gggcggctcg aaggtcaagg tccgctgacc ctcgctgagc ttgccgccac
     1441 gttcggcctt cagcccgagc ggatttccac cgcgcttacc tccctcgaag tggaaggctt
     1501 tgcgctcgct ggctccttca cgccgggcgg gagcgagcgc gaatggtgcg agcggcggct
     1561 gcttgcgcgc gtccatcgct atacggtgaa gcggctgcgc gccgagatcg agcccgtctc
     1621 cgcgcgggac tatctccgct tcctgttcga ttggcagggc gtctctccag atggccgccg
     1681 cgaaggcgcg caggctctcg atgcggtggt cgagcagctc gcaggcttcg aagcgccggc
     1741 agttgcatgg gagggttcga ttttgccggc gcggctttcg cactatgagc caagtctcct
     1801 cgacgatgcc tgccttgcgg ggcgtatcgc ctgggcgcgg atcggggcac ctgcgccgaa
     1861 gtcgaaatcg aacggcaggc gctccgtccc gctgaaatcc acgccgatta gtctgttccc
     1921 gcgcaaggcc gccggcgtat ggccgcctgc tgatgaggct ccccaggtga gcgccagagc
     1981 cgcgaaggtc gcggcgttca tcgccgagaa cggcgcctcc ttcttcgacg agatcgtcga
     2041 cggcacgcat ctcctacgca cgcagataga ggaagcgctg gccgaacttg ttgctgctgg
     2101 tcaggtcatc tccgacagct tcggcggatt gcgcgcgctg ctcacccctg cccgccacca
     2161 caagcggcgg cccaacggcg cgcgtctggc gggcgcggga agatgggcgc tcgccaagcg
     2221 taaggccgcg aacggcgcgg ccaaaacgga tgacgtggag cgcgtggaag agatcgcgct
     2281 cgctctactg cgccgctacg gcgtcgtgtt catgcgaatg ctggagcgcg aggcgtcaag
     2341 gctgccgaaa tggcgcgttc tgttgcgcgt ctatcgcaag ctcgaggcgc gcggtgatat
     2401 ccggggcgga cgcttcgtcg cgggcttttc cggcgagcag ttcgcgctgc ccgaagccgt
     2461 cgcagcattg cgttcgatca ggcgacgcgc gccggatgac agtcttatct cggtttctgg
     2521 cgctgatccg cttaactttg ccggcattct tacgccgggg gcgaaactcg cggggctcgc
     2581 cgggaaccgg gtgctatatc gagacggcat cccgatcgcc ttcctcgaag gggacgcgac
     2641 ccgcctactc gagccggtcg agccggagat tgaggctatg gcgcgggcag cgctgcacgg
     2701 ccacgccaag ccggccgcgc atctcccacg cttgcgccga cttgcggaac tcgaccaggc
     2761 ccggaggcat cgcggtcgtg ctggaccgtt cgcggcctga gcgtcatcgg tgtacggagc
     2821 tcatcgtaaa cggagtcact cgcagcgtcc ccgagcaagc gtcaagacgg cccggaaggg
     2881 cactgcgagc gtaaagtcga ctgcgcgttc cgcctttcat ctcacgaccc gaaggaaggt
     2941 attgaagcgc tcccttgttg taagccggtg ctatccgtgc agcttttctg gtatctcggc
     3001 gccttcaaat gtccgctttg agtcagaagc tgatgcgttc agcggatttg aatgtctgct
     3061 tggtgccatt tgcggacgtc acatttacag gcattcgata tgtcgaaagc cgatcatccc
     3121 cagcgccctg atccggtcga gattcgcagc tgcggcggcg acgctcgaca gttctccccg
     3181 tcagctgcgc gcaattgtgg gccaatccga gaggtgctta ttcgggttct gccgaagaag
     3241 ggcatcgcgc tcgaaattgg atccggtacc ggagagcatg tcatcgcttt cgcgaaggcg
     3301 ctgcctgggc tcgtctggct gccgagcgat cccgacgcgg cctcgcgcgc cagcatcaag
     3361 gcgtggaggg ccactgaggg gctcaccaat gtgcaggcgc cggtcgccat cgacgtgcgc
     3421 gaacgggtat ggggcgtcga ggacgccgca ccatttgacg cgatgatctc cttgaatatg
     3481 gtccacatcg cgccttggga gtcagcgctc gggctcctcg ccggtgcggg gcgcctcttg
     3541 aggccggacg gggtcctcgt cttctatggg cccttcatgc tgggcgggat acacacggcg
     3601 gcgtc
//
LOCUS       DATDMP010000361         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1058296, whole genome shotgun sequence.
ACCESSION   DATDMP010000361 DATDMP010000000
VERSION     DATDMP010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058296"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..366
                     /locus_tag="VI195_06330"
     CDS             115..366
                     /locus_tag="VI195_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY6824039.1"
                     /translation="MSDRHFFDDLSERINEAIRNSPAADIEKNLRALLADWFGRMDLV
                     MRQDFDVQRKLLEQALARLATLEGRVAELERRANNVDPR"
     gene            414..1913
                     /locus_tag="VI195_06335"
     CDS             414..1913
                     /locus_tag="VI195_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY6824040.1"
                     /translation="MTVATVSSRALSGVQAPEVTVEVHLGPGLPAFHIVGLPDTEVRE
                     ARDRVRAALNHAHFEFPARRITVNLAPAELPKDSSRFDLPIALGILAASGQLEPQALD
                     GQEFAGELSLTGELRPVRGALAMALSARSAGRAFVLPEGNAPQAALAQGAQILPARSL
                     LEVVGHLAQEARLAPYCGETPVMRAGYADFNDVKGQQQAKRALEVAAAGGHSVLMIGP
                     PGTGKSMLALRFPGILPPMSEVEALEVAAVHSVSPHGFDATRWGERPFRAPHHSASAA
                     ALVGGGHLPRPGEISLAHQGVLFLDELPEFDRDVLEALREPIETGRVAISRAARQVEF
                     PARFQLIAAMNPCPCGHCGDRSGRCRCTPERIARYRGRISGPLADRIDIKLEVPAPRE
                     AELTATVPGESSGDIAKRVARARQAQLARQAKPNALMGTREIDRHCATGREGGELLRH
                     ALARLLLSPRAYHRVLRVARTIADLAGASAVGPEHVAEAIQYRRIDASF"
     gene            1920..>2107
                     /locus_tag="VI195_06340"
     CDS             1920..>2107
                     /locus_tag="VI195_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY6824041.1"
                     /translation="MATYAIGDVQGCFDELRALLDKIGLGAGDRLWFVGDLVNRGPKS
                     LEVLRFVRSLGEQAIVVLG"
BASE COUNT          303 a          699 c          732 g          373 t
ORIGIN      
        1 cgtgcggctt caatggcttc ccggtttgcg acgggttcat tgcgccatat tggtgcggcc
       61 ggcgggtggc tccgcacgca cgcggtgcgc agcccgcggt ggtaccttgc cgctatgagc
      121 gatcggcatt ttttcgacga cttgagcgag cgcatcaacg aagccatccg taactcgccg
      181 gcagcggaca tcgagaagaa tttgcgtgcg ctgctggccg actggttcgg gcggatggac
      241 ctagtgatgc gccaggactt cgacgtgcag aggaagcttc tcgagcaggc actcgctcgt
      301 ctggcgacgc tggagggacg cgtcgccgag ctcgagcgcc gcgccaataa cgtcgatcct
      361 cggtaaacgc ttcgcacgcg tgggcgttgc tcgcctcggg gagatggcga gccatgactg
      421 tcgcgaccgt atcgagccgt gcgctttcgg gtgttcaagc gcccgaggtg accgtcgagg
      481 tgcatctcgg gcctggtttg cccgccttcc acattgtcgg actaccggat acggaggtgc
      541 gtgaagcgcg cgatcgcgtg cgcgcggcgc tcaatcacgc ccacttcgag tttccggcac
      601 ggcgcatcac cgtcaatctg gcgcccgccg agctgcccaa ggactcgagc cgtttcgact
      661 tgccgatagc cctcggcatt ctggccgcga gcgggcagct cgagccgcag gccctcgatg
      721 ggcaggaatt cgccggcgag ctctcgctca ccggcgagct gcgcccggtg cgtggcgcgt
      781 tggcgatggc gctgtcagcg cgcagtgcag gtcgcgcgtt tgtattgccg gagggcaacg
      841 cgccgcaggc cgcgctcgcg cagggtgcgc agatcctccc cgccaggtcg ctgctcgagg
      901 tcgtgggtca tctcgcgcag gaggcgcgtc ttgcgccgta ctgtggcgag acgccggtga
      961 tgcgcgccgg ctatgccgac ttcaacgatg tgaagggcca gcagcaggcc aagcgcgcgc
     1021 tcgaagttgc ggcggccggc ggccatagcg tcctgatgat cggaccgccg ggcaccggca
     1081 aatccatgct ggccttgcgc ttccccggca tcctgccgcc catgtccgag gttgaggcgc
     1141 tggaagttgc ggccgtgcac tcggtgtccc cgcacggctt cgatgcgacg cgctggggcg
     1201 agcgtccttt ccgcgcgccc catcacagtg cttcggcggc ggcactggtg ggcggcggcc
     1261 atctgccgcg gcctggggaa atttcgctcg cacatcaggg cgtgctgttt ctggacgagc
     1321 tgccggagtt cgaccgcgac gtgctggaag cactacgcga gccgatcgag accgggcgcg
     1381 tggccatctc tcgcgcggcg cgccaggtgg aattcccggc gcgcttccag ctgattgccg
     1441 ccatgaatcc ctgtccctgc ggccactgcg gcgatcgcag cggccgctgc cgctgcacgc
     1501 cggagcgtat cgcgcgctac cgcggacgca tctcgggccc actcgccgat cgcatcgaca
     1561 tcaagctcga agtgccggca ccgcgggaag ccgagctgac agctactgtc cccggcgaat
     1621 cgtcaggtga cattgcaaag cgcgtcgccc gcgcgcgtca ggcgcagctc gcgcgccagg
     1681 cgaaacccaa tgcgctcatg ggcactcggg agattgaccg ccattgcgcc accggccgcg
     1741 agggcggcga gctcctgcgg cacgcgctcg cgcggttatt gctgtcgccg cgcgcctacc
     1801 atcgcgtgct gcgcgttgcg cgcactatcg ccgatctcgc gggcgcgagc gctgtcggtc
     1861 cggagcacgt cgccgaagcg atccaatatc ggcggatcga tgctagcttc tagcaggcga
     1921 tggcgaccta tgcgatcggc gacgtgcaag gctgcttcga tgagctccgc gcgctgctcg
     1981 acaagattgg tttgggcgct ggcgatcggc tctggtttgt tggcgacctc gtaaaccgcg
     2041 gcccgaaatc gctcgaggtc ctgcgattcg tgcgctcgct tggcgagcag gcgatcgtcg
     2101 tcctggg
//
LOCUS       DATDMP010000362         3376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_725401, whole genome shotgun sequence.
ACCESSION   DATDMP010000362 DATDMP010000000
VERSION     DATDMP010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725401"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1775)
                     /locus_tag="VI195_06345"
     CDS             complement(111..1775)
                     /locus_tag="VI195_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6824042.1"
                     /translation="MTNCSTLRIAIASATLLPLYALTGAYAADAAAAADAGAAPGNKD
                     ELQEVVVYGMTANLEKSLEIKRTAPVVMDSINSVELGRFPDADVADSLAHLPGITLER
                     TTGGEGIRISIRGLGVGYNIVTLDNRILASDDDSRDLAFDVLPAELITGADVLKSPQA
                     SAVEGSIGGTVNLRRASAFDNPGLHAGAHAEGNYNDMSELYGQKYSLFVENTFAEQTL
                     GFVLGGVHSNDHVRTDSLNAYNQNIYGPTIYPFTANPGDPGSVPLTATPCCITFGSIF
                     DNKKRDALSGSLEWRPNSAFTLKVDGLWTHLDDPQIGYNQSYYFAAPDSTGFYGDGTS
                     WADNVVVKNGVITSLSVNEFQPEMVNNTQNRKVDTYLYGLNASWKPTETLSFAFDGYR
                     STASRPEGGQDTFVTAGLVNAQPTAEDILNFTDVAHSLPNINVVVPPSQLGLTACPSG
                     SASTTNPGFCSYTTLMNSGFLNNNKYWSTHYVGLNGYSVHDEVVGLNLDGAWNVDYGF
                     WQRLLFGVGYTDRKKDRDDSSNDWTNGSGQYGTLYQTAGCPVQCNP"
     gene            complement(1772..3085)
                     /locus_tag="VI195_06350"
     CDS             complement(1772..3085)
                     /locus_tag="VI195_06350"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-tagatose-bisphosphate aldolase, class II,
                     non-catalytic subunit"
                     /protein_id="HEY6824043.1"
                     /translation="MKALLELLRRHRAGEPVGIYSVCSAHPLVLRAALQHAHALGGHA
                     LIEATSNQVNQEGGYTGLRPAEFRDVVGRMADEVGLPRERVLLGGDHLGPNCWQVQPA
                     QAALQRAGVMVAEYVRAGFRKIHLDCSMSCGDDPPALSAELIAERAALLCAQAENAWQ
                     SAGGEAPLYVIGSEVPVPGGAQEALHELAVTEPQAALASIATHRAAFERCGLTAAWER
                     VIALVVQPGVEFDQQHVVEYTPSRAQPLSACLESVPRLVFEAHSTDYQTREALAALVR
                     DHFAILKVGPALTFALREAVWALDQIEREWLGDEKSSRVRETLLAAMRSDPQHWRKYY
                     QGSGRTLELQLAYSLSDRIRYSWPAASVTRALAELEASFDAGTPPPALLRQYLPLAYG
                     AVRRAELRPSAQNLIVHHVRQVLSEYSWACGQRADAGAQNGGITQ"
     gene            complement(3082..>3376)
                     /locus_tag="VI195_06355"
     CDS             complement(3082..>3376)
                     /locus_tag="VI195_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824044.1"
                     /translation="AVWMTDFSQPINYGLGGPWLAAITQILNSIGALTIVYAFRYGKA
                     MVVSPLTNAGAPLITALIALALLGIVPQPLKLTGIGLAFLAAVLLALEPEDGK"
BASE COUNT          565 a         1068 c         1149 g          594 t
ORIGIN      
        1 tggctggcgc tgagctgcgg cagcacgacc gggtaggagc cgccagcgcc ctgcatgaag
       61 tgcggaatgc tcacgttcga gatgacattg aatccctgcg aagcgaacga ctaggggttg
      121 cactgcaccg gacagcccgc ggtctgatag agcgtgccgt actgcccgga gccgttggtc
      181 cagtcgttgc tgctgtcgtc gcggtccttc ttgcgatcgg tgtagccgac gccgaacagc
      241 agccgctgcc agaacccgta gtcgacattc caggcaccgt caaggttcaa gcccacgacc
      301 tcgtcgtgca ccgaataacc gttgagaccc acgtagtgcg tggaccagta cttgttgttg
      361 ttgaggaacc ccgagttcat gagcgtcgtg taggagcaga agcctgggtt ggtcgtgctc
      421 gcgctgccgg atggacaggc cgtgagaccc agctgactcg gcggcactac gacgttgatg
      481 ttcggcaggc tgtgagcgac gtcggtgaaa ttcaggatgt cctcggcggt gggttgggca
      541 ttaaccaggc ccgccgtgac gaacgtgtcc tgacccccct cgggtcggct ggcggtggag
      601 cggtagccgt cgaaggcgaa gctcagcgtc tccgtcggct tccagctggc gttgagtccg
      661 tagagatagg tatcgacctt gcgattttgg gtgttgttca ccatctccgg ctggaactcg
      721 ttgaccgaca gcgaggtgat gacgccgttc ttcacgacca cgttgtccgc ccaggaagtg
      781 ccgtcgccgt agaaacccgt gctatcgggc gccgcgaagt aatacgactg gttgtagccg
      841 atctgtggat cgtcgaggtg cgtccacaac ccgtccacct tgagagtgaa cgccgaatta
      901 gggcgccatt cgagactccc ggagagcgcg tcgcgcttct tgttatcgaa tatggagccg
      961 aaggtgatgc agcacggcgt tgccgtgagt ggcaccgacc ccgggtcccc cggattcgca
     1021 gtgaacggat aaatcgtggg cccgtagatg ttctggttat aggcattgag cgagtcggtg
     1081 cggacgtggt cgttcgaatg caccccgccg agcacgaatc cgagcgtctg ctctgcgaag
     1141 gtgttttcga cgaacagcga gtacttctgc ccgtaaagct ccgacatgtc gttgtagttg
     1201 ccctccgcat gcgcgccggc gtgaagccca gggttgtcga aggcgctcgc ccgacgcagg
     1261 ttgaccgtgc cgccgatgct gccctccacc gcggaagcct gcggcgactt cagcacgtcg
     1321 gcgccggtga tcagctccgc cggcagcacg tcgaaggcga ggtcgcgact gtcatcgtcg
     1381 cttgccagga tgcgattatc gagtgtgacg atgttgtatc cgacgccgag gccccggatg
     1441 ctgatgcgga tgccctcgcc gccggtggta cgctcgagcg tgattccggg gaggtgagcc
     1501 agtgagtcgg cgacgtccgc gtccggaaaa cgccccagct cgacggagtt gatggagtcc
     1561 atcaccacgg gcgcggtccg cttgatttcg agcgatttct cgaggttggc cgtcatgccg
     1621 tagacgacga cttcctgaag ttcatccttg ttgccgggtg ctgcgccggc gtctgcggcc
     1681 gcagccgcat cggcggcgta agcgccggtg agtgcgtaca gtggcagcag cgtcgcgctc
     1741 gcgattgcga tccgtaaagt tgagcagttc gtcattgtgt gattccccca ttttgtgcgc
     1801 cggcatccgc ccgctgaccg caggcccatg agtactcgct cagcacctgg cgcacgtggt
     1861 gcacgatgag attctgcgca ctcggccgga gctcggcgcg tcgcacggcg ccgtaggcca
     1921 agggcaggta ctgacgcagc agcgcaggcg gcggcgtgcc ggcgtcgaaa gaagcctcga
     1981 gctcagccag cgcgcgcgtc accgaggcgg ccggccagct gtagcggata cggtcgctca
     2041 ggctgtaggc gagttgcagc tcgagcgtgc gtccgctgcc ctggtaatac ttgcgccagt
     2101 gctgcgggtc gctgcgcatg gctgcgagca gcgtctcgcg cacgcgtgag ctcttctcat
     2161 ccccgagcca ctcgcgctcg atctgatcga gtgcccacac cgcctcgcgc aacgcaaaag
     2221 tgagcgccgg gccgaccttg agtatcgcga aatggtcgcg caccagtgcg gcgagggcct
     2281 cccgtgtctg gtaatcggtc gaatgcgcct cgaacaccag tcgcggcacc gattcgaggc
     2341 aggcgctgag cggctgtgcc ctggaaggcg tgtactcgac cacatgctgc tggtcgaatt
     2401 cgacgcccgg ctgcaccacc agcgcgatca ctcgctccca ggcggccgtg aggccgcagc
     2461 gctcgaaagc cgcgcgatgc gtggcaatgc tggccaatgc ggcctgcggc tcggtgacgg
     2521 cgagctcgtg cagtgcctcc tgcgcgccgc ccggcaccgg cacctcggag ccgatcacgt
     2581 agagcggcgc ctcgccgccc gcgctctgcc aggcgttctc ggcctgcgcg cagagtagcg
     2641 ccgcacgctc ggcgatcagc tcggcggaca atgcgggtgg gtcgtcgccg caggacatgg
     2701 agcagtcgag gtggatcttg cggaaaccgg cacgcacgta ctcagcgacc atcacgccgg
     2761 cgcgctgcaa ggcggcttgc gctggctgca cctgccagca gttgggcccc agatggtcgc
     2821 cgccgagcag cacgcgctcg cgcggcagcc cgacctcatc cgccatccgg cccacgacgt
     2881 cgcgaaactc cgcggggcgc agaccggtgt agccgccctc ctggttcacc tggttggagg
     2941 tcgcctcgat cagtgcatgg ccgccgaggg cgtgggcgtg ttgcaacgcc gcccgcagca
     3001 cgagcgggtg cgccgagcag accgagtaga taccgaccgg ctcaccggca cggtggcgcc
     3061 gcagcagctc gagcagcgct ttcacttgcc gtcctccggc tcgagggcga gcagcaccgc
     3121 ggcgaggaag gcgagcccga tcccggtcaa cttgagcggc tgcggcacga tgccgagcag
     3181 cgcgagcgcg atgagcgcag tgatgagcgg agcgcccgca ttggtgagtg gcgaaaccac
     3241 catcgccttg ccatagcgga aggcgtacac gatggtgagc gcaccgatgg agttgaggat
     3301 ctgtgtgatc gcggcgagcc acgggccgcc gaggccgtaa ttgatcggct gtgagaagtc
     3361 cgtcatccac acggcc
//
LOCUS       DATDMP010000363         5569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_392236, whole genome shotgun sequence.
ACCESSION   DATDMP010000363 DATDMP010000000
VERSION     DATDMP010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392236"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VI195_06360"
     CDS             <1..769
                     /locus_tag="VI195_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824045.1"
                     /translation="ARRVLQRARDALEVAAQQPEREGQREAGVGEDQDPPGVEQVRAA
                     ARARAELLTPIIKGWCTETGNEVAAIGVQVHGGMGFIEETGAAQYMRDVRITTIYEGT
                     TGIQSNDLIGRKLARDRGAAMSALLADMERELGATAGGDAKVEAVRRAALEGVSFLGR
                     ATRSLLEALASRPERAMAVSVPYLKLCGYVTGGWLLAKSAAIAAARAQGAERDFYTAK
                     VHTAAFYAAQVLPAAAALVRVVESGGASVIGTDATLL"
     gene            845..1342
                     /locus_tag="VI195_06365"
     CDS             845..1342
                     /locus_tag="VI195_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6824046.1"
                     /translation="MPDTPLRFDHLALPVFDAASTLHFYSEVLQLPLVDAISGDDWGG
                     KPWLMMLFGTGDGQLLAFCALHGAQPPRPDGLPADAWHYAFSVSSAAEQEKWRSRLRE
                     HGIAFREEDHGTQHSIYFSDPNGIVLEVTTPASRADLQPDAHAAQRVRRWIAAQSGSG
                     AAGPE"
     gene            1339..2244
                     /locus_tag="VI195_06370"
     CDS             1339..2244
                     /locus_tag="VI195_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6824047.1"
                     /translation="MNRRELLGRAVTLLSAAAVSSPGARASSTDLIVRAIPASGEELP
                     VIGLGTSGPFEVGESRGERAPLEKVLQAFFAAGARLIDTSPMYSSAERVLGELLTDDL
                     RRHAFLATKVWTRGERAGVEQMTRSAQLLRREHLDLIQVHNLLDLDTQLKTLYAWKQA
                     GRVRYLGITHYTVSAHADLARALEHHRLDFVQLNYSPLTRAAEQRLLPLAAERGVAVL
                     VNRPFEDGALFAAVRGRPLPAWAADIDAGSWGQLALKFIAAHPAVTCIIPATGSLAHL
                     QDNLAGGRGRLPDARERAAIAQAFA"
     gene            complement(2281..3615)
                     /locus_tag="VI195_06375"
     CDS             complement(2281..3615)
                     /locus_tag="VI195_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6824048.1"
                     /translation="MLANDSSLDHITPYDRFAWSDVQIEQWLASGTRRRELTDYFGLR
                     EYRVLAGLARRALAAPAAAGAAQVLIVPGIMGSQLGIARAAPLPSDILWLDPIDIQRG
                     RLAALRMAAAAPIVPLGVVLFSYLRLKLQLRARGFAVEFHDYDWRLPVARAGQQLAEK
                     LAALRPARVAIVAHSMGGLVCRAALALYTASHVERVVLLGTPNCGSFAAVQALRGTYA
                     VVRKVARLALQTSAESLAAEVFGSFPSLYELLPSGSCDLAGLDLLDAQAWPAAAPLPD
                     PALLEAARTLPQRLAPADERFAVIAGVGEETVTALRRRGEEFLYTVTRGGDGTVPLAS
                     AQLSGARCFYARVAHSDLTRDAQVAAAVVDLLRSGNTRRLPTRRTGGGRARARVSDGE
                     LRRTHCEKVDWAALTPRERRLFLENLNEPPQLRLRAPRSASPRRRTRRGRHP"
     gene            complement(3632..4078)
                     /locus_tag="VI195_06380"
     CDS             complement(3632..4078)
                     /locus_tag="VI195_06380"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY6824049.1"
                     /translation="MRVSALPAACLVVLAGLLPATGFAQPPGLSAQDAWIRATPGIDV
                     AAAYLTLHNGGTQPVVVSGVRCSDAAAAMIHQTTIVNGQSAMRPREPLRIAPGETVRF
                     APDGLHIMLQGLKRPLAAGEEVPLVLLLEGAGNLTVMARVRALADD"
     gene            complement(4247..4690)
                     /locus_tag="VI195_06385"
     CDS             complement(4247..4690)
                     /locus_tag="VI195_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824050.1"
                     /translation="MKKSALLLLLAAILTISTSVTSAQEEHAYTQGHVVVVSFVRTEP
                     GMFEEYLRYLDKTYKPLMEGYKKQGIIVDYAIYQSLPRDAHDADVILTVTYKNMAALD
                     DLQSRTDPGVKQTFGSLAKAASASADREKLRKQLGSQLVRQLVLK"
     gene            complement(4805..>5569)
                     /locus_tag="VI195_06390"
     CDS             complement(4805..>5569)
                     /locus_tag="VI195_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6824051.1"
                     /translation="NGATGFRREFYVNFAAYCAARGYHTLVYDYRGMGVSARAPLGGE
                     SARMSDWGRFDMPGALESLAQRYPHLPLFTLGHSVGGQLLGCMANQARARAHVMIATA
                     TGYWRRQRVPFRYQALLFWKVYGPLMLQRRGYVPRGVLWRGEPLPPGVFLQWRRWCLS
                     PAGFGSALDEDMSDAGFAAVHAPLLSWGFTDDPIATPTAVAALLASYPNACIERRWTT
                     PAEAGVRVIGHHGFFAERHRDSLWRAALDWLDARRG"
BASE COUNT          884 a         1912 c         1926 g          847 t
ORIGIN      
        1 cgcgcgccgc gttctccagc gagcgcggga tgctctcgaa gtagccgcgc agcagccaga
       61 gcgtgaaggg cagcgcgaag ctggtgtagg cgaagatcag gatccaccag gtgttgagca
      121 ggtgcgcgcc gcggcccgcg cgcgcgctga actactgacg ccgatcatca aaggttggtg
      181 caccgaaacc ggcaatgagg tggccgctat cggcgtgcag gtgcacggcg ggatgggctt
      241 tatcgaggag accggcgccg cgcagtacat gcgcgatgta cgcatcacca cgatctatga
      301 ggggaccacc ggcatacagt cgaacgatct catcggccgc aagctcgctc gcgaccgcgg
      361 tgcagcgatg agcgcgctgc tcgctgacat ggaacgcgag ctcggcgcca ctgccggcgg
      421 cgatgcgaag gtcgaggcgg tacgccgcgc ggccctcgag ggcgtgagct ttctcggtcg
      481 cgccacgcgc tcgcttctcg aagcgctcgc gagtcggccc gagcgcgcca tggcggtgtc
      541 ggtgccgtac ctgaagctct gcggatacgt caccggcggc tggctgctgg caaagtccgc
      601 ggccatcgcg gccgcccgcg cccaaggcgc ggagcgcgat ttctacaccg ctaaggtcca
      661 cacggcggcg ttctacgccg cgcaggtgct gccggccgca gctgcactcg tgcgcgtggt
      721 cgagtcgggg ggcgcgagcg tcatcggaac cgacgcgacc ttgctgtagg gtggcgtgcg
      781 gcgcaagaca gtacgcagcg gcgcgtgcga agatctttcg atctcagctc gcggacgcgc
      841 ccccatgccc gacacgccgc tgcgcttcga tcatcttgcc ctgccggttt tcgacgcggc
      901 gagcacgctg cacttctaca gcgaggtgtt gcaactgccg ctggtggacg cgatctcggg
      961 agatgactgg ggtggtaagc cctggttgat gatgctgttc ggcaccggcg acgggcagct
     1021 cctggcattc tgcgcactgc acggcgcgca gccgccacgg cccgatggcc tgcccgcgga
     1081 cgcgtggcac tatgcgttct cggtgtcctc cgccgccgag caggagaagt ggaggagtcg
     1141 gctgcgtgag cacggcatcg ccttccgcga agaggaccac ggcacgcagc attcgatcta
     1201 cttctccgat ccgaacggca ttgtgctgga agtcaccacg ccggcgagca gggccgatct
     1261 gcagcccgat gcgcacgcgg cgcagcgcgt gcggcgctgg atcgcggcgc agtccggcag
     1321 cggcgctgcc ggaccggaat gaatcgccgt gagctcctcg gtcgcgcggt caccctgctg
     1381 agcgccgccg ccgtgtcgtc gcccggcgcc cgtgcgagca gtaccgacct catcgtgcgc
     1441 gccatccccg cgagtggcga ggagctcccc gtgatcgggc tcggcacctc cggacccttc
     1501 gaggtgggcg aatcgcgtgg cgagcgggcg ccgctcgaga aggtgctgca ggccttcttt
     1561 gcagcgggcg cgcgtctcat cgacacatcg cccatgtact cgagcgccga acgcgtgctc
     1621 ggcgagctgc tgacggacga tctgcgccgg cacgcattcc ttgccaccaa ggtgtggacg
     1681 cggggcgagc gcgccggggt cgagcagatg acgcgatcgg cgcagctact gaggcgcgag
     1741 cacctcgatc tcatacaggt gcacaacctg ctcgatctcg acacgcagct gaagacgctc
     1801 tacgcctgga agcaggccgg gcgggtgcgc tacctcggca ttacgcatta cacggtgagc
     1861 gcgcacgccg atctggcgcg agccctcgag caccatcgtc tcgatttcgt gcagctcaac
     1921 tattcaccgc tgacgcgtgc ggcggagcag cgcctgctgc cactcgcggc cgagcgcgga
     1981 gtggcagtgc tcgtgaatcg acccttcgag gatggcgcac tgtttgccgc cgtgcgtggc
     2041 aggccactgc cagcctgggc ggcggacatc gatgccggca gctggggtca gctcgcactg
     2101 aagttcatcg ctgcacaccc ggccgtcacc tgcatcattc ccgccaccgg cagcctcgcg
     2161 catctgcagg acaacctcgc cggtggccgt ggacggctgc ccgatgcgcg cgagcgtgcg
     2221 gccatcgccc aggctttcgc ctgaagcgca ctctgccagg ggcgtgcgag cttggtgctc
     2281 tcaggggtga cgcccgcgcc gcgtgcgccg ccggggagag gcgctgcgtg gggcgcgcag
     2341 ccgcagctgc ggaggctcgt tgaggttctc gaggaacagt cgccgctccc tcggagtcag
     2401 cgcagcccaa tcgacctttt cgcagtgcgt gcggcgcagc tcgccatcgc tcacccgggc
     2461 acgcgcgcgc ccgccaccgg tccgccgcgt cggcagccgc ctcgtgttac cgcttctgag
     2521 cagatccacc accgccgccg cgacctgcgc atctcgtgtc aggtcgctgt gcgcgacacg
     2581 ggcgtagaag caacgcgcgc cggaaagctg cgcgctcgcg agcggcacgg tgccatcgcc
     2641 gccgcgcgtg acggtgtaga ggaactcctc cccgcgccgg cgcagcgcgg tgaccgtttc
     2701 ctcgcccacg ccggcaatga ccgcgaagcg ttcatccgcc ggcgcgagcc gctgcggcag
     2761 tgtgcgcgcc gcctcgagca gcgccgggtc cggcaacggg gcggctgctg gccaggcctg
     2821 cgcatccaac aggtcgagac cggcgaggtc gcaggatccc gagggcagca gctcgtaaag
     2881 gctcgggaag ctgccgaaga cctcagcggc cagtgactca gcggaagtct gcagcgcgag
     2941 gcgcgccacc ttgcgcacca cggcgtaggt gccgcgcagc gcctgcacgg cggcgaacga
     3001 gccgcagttc ggtgtcccca gcagcaccac gcgctccacg tgggaggccg tgtacagggc
     3061 gagcgcggcg cggcacacga gcccgcccat gctgtgcgcc acgatcgcca cccgtgccgg
     3121 tcgcagcgcc gcgagcttct cggccagctg ctgtcccgcg cgcgccaccg gcagccgcca
     3181 atcgtaatcg tgaaactcga ctgcgaagcc tcgcgcgcgc agctgcagct tcagccgcag
     3241 atacgagaac agcactacac cgagcggcac gatgggcgca gcggctgcca tgcgcagcgc
     3301 cgcgagccgc ccacgctgaa tgtcgattgg atcgagccag aggatatcgc tcggcagcgg
     3361 cgccgcacgc gcgataccga gctgcgagcc catgatgccg ggcacgatga gcacctgcgc
     3421 tgcgccggcc gcagcgggcg ctgccagcgc gcggcgtgcg agcccggcga ggacacggta
     3481 ctcgcggaga ccgaagtagt cggtgagctc gcgccggcgc gtgccgctcg cgagccactg
     3541 ctcgatctgc acgtcactcc atgcaaagcg gtcgtaggga gtgatgtggt cgagtgatga
     3601 atcgttcgca agcacgcgca gcgccgtcga ctcagtcgtc cgcgagcgcg cgcacgcgcg
     3661 ccatcaccgt gaggttgccg gccccttcga gcagcagcac cagcggcacc tcctcgccgg
     3721 ccgcgagcgg ccgtttgagc ccctgcagca tgatgtgcag gccatcaggc gcgaagcgca
     3781 ccgtctcacc gggcgcgatc cgcagcggct cacgtgggcg catggccgat tgcccgttga
     3841 cgatcgttgt ctggtgaatc atcgcggcgg cggcgtcaga gcagcgtacg ccgctcacca
     3901 ctaccggttg cgtgccgccg ttgtggagcg tcaggtaggc ggccgccaca tcgatccccg
     3961 gagttgcacg gatccatgca tcctgcgcgc tgagccccgg cggctgcgcg aagcctgtcg
     4021 ccggcagcag gccggcgagc acgacgaggc aggcggcagg gagcgcggaa actcgcaacg
     4081 gtcgcctcct gcttggcacg cgtaagcgct gaggatgcga gggcgattat gccacttgag
     4141 tacgcggcta ccgcagccgc gcctgttccg cagcggcgcg ctggcgctac cgctgcgggg
     4201 cgagatcacc cccgtgctgc cgccgggggt gcgctcgccg cctgagctat ttcagtacca
     4261 actgccggac caactgactg ccgagttgct tgcgcagctt ctcgcgatcg gccgaggccg
     4321 aggccgcctt cgccagggat ccgaaagtct gcttcacccc gggatcggtg cgcgactgca
     4381 agtcatcgag cgccgccatg ttcttgtagg tcacggtcag gatcacgtcc gcgtcgtgcg
     4441 catcgcgcgg cagcgattga tagatggcat agtcgacgat gatgccctgt ttcttatatc
     4501 cctccatgag cggcttgtag gttttgtcga gatagcgcag gtattcctcg aacatgcccg
     4561 gctcggtgcg cacgaatgac acgacgacga catgcccctg cgtgtaggca tgctcctcct
     4621 gggcactcgt caccgatgtc gagatcgtca ggattgcggc taacagcagc agcagtgcgg
     4681 acttcttcat gtggctatcc cctttttgct ggccgatgaa cggcagagcg gttgttgttg
     4741 gcgcggcaga ctatactcgt ggtgcgcgcg tgccgctcgg tgtttcacga tttgcggaca
     4801 catctcaacc ccggcgtgca tcgagccagt cgagcgctgc gcgccagagc gaatcgcggt
     4861 gtcgctcggc aaagaacccg tgatgaccga tgacgcgcac tcccgcctcg gccggcgtgg
     4921 tccagcgccg ctcgatgcac gcgttcggat atgaggcgag cagcgccgcg accgccgtcg
     4981 gtgtcgcgat cggatcgtcg gtgaagcccc aggaaagaag cggcgcgtgc accgcggcga
     5041 agccggcatc gctcatgtcc tcatcgagcg ctgagccgaa gcccgccgga ctcaggcacc
     5101 agcgccgcca ctgcaggaac actccgggcg gcagcggctc gccccgccac aacacaccgc
     5161 ggggcacgta gccgcgccgt tgcagcatca gcggtccata caccttccag aacagcagcg
     5221 cctggtagcg gaacggcacc cgctggcggc gccagtagcc ggtcgcggta gcgatcatga
     5281 cgtgcgcgcg ggcgcgtgcc tggttggcca tgcagccgag tagttgaccg ccgacgctgt
     5341 ggccgagcgt gaacagcggc aggtgcgggt agcgctgcgc gagtgattcg agtgccccag
     5401 gcatgtcgaa ccggccccaa tcgctcatgc gcgcgctctc gccgccgagc ggcgcgcgtg
     5461 ccgagacacc catgccgcgg tagtcgtaca cgagcgtgtg atagccgcgc gcggcgcagt
     5521 aggccgcgaa attgacatag aactcgcgcc tgaagccggt ggcgccgtt
//
LOCUS       DATDMP010000364         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1836150, whole genome shotgun sequence.
ACCESSION   DATDMP010000364 DATDMP010000000
VERSION     DATDMP010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836150"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VI195_06395"
     CDS             complement(<1..555)
                     /locus_tag="VI195_06395"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY6824052.1"
                     /translation="MIPRIALTSGEPAGIGPELCLALATRQLPCELVCLGDRMLLEQR
                     AAQLRLAVRLCDYPGAAGPQAAAERRGELCVAHVPLAAPSIAGRPDPANARYVLALLD
                     RAIEGTLAGEFHALVTAPVHKGVINDAGIVFSGHTEYLQRRTHAQRAVMLLVAGALRV
                     ALATTHLPLRAVSDAISTDLLCEVA"
     gene            complement(565..1890)
                     /locus_tag="VI195_06400"
     CDS             complement(565..1890)
                     /locus_tag="VI195_06400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6824053.1"
                     /translation="MRQTAISSIPMWMVLGLGALAVHGGAQAQTRELATQGELLDRVV
                     AIVNDGVVLNSDLDAQIGSVSERLRQQKLELPPQNVLRQQVLERLVLQEIQLQRANHA
                     GVKVSDEMLNSALQDVAKRNGLTLTQLPEALARQGGDYAEYREEIRREMMLQMLRQRD
                     VLQHISVTPREIDQFLERQSKAPSERNEYNVSHILIAVGQEATAGQQEAAARRAQEVF
                     ERARGGEDFAKLAVAYSNSQTALEGGALGWRKGSELPTFLADIIAKLKPGEVSEPLRT
                     PTGYHIIRLNEIRGGTENAVEDQVHVRHILMKTNELADDATVRGKLEALRERILKGED
                     FAAIAQITSQDPGSAAEGGDLGWSGPGTFAPEFEQAIATLKDGEISEPFRSQFGWHIA
                     QMLGHRRFDNSDEIKRRQAMEAIRASKADEETELWLRRMRDEAYVEYKS"
     gene            complement(1908..>2725)
                     /gene="lptD"
                     /locus_tag="VI195_06405"
     CDS             complement(1908..>2725)
                     /gene="lptD"
                     /locus_tag="VI195_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY6824054.1"
                     /translation="IPDLNPVQLFRTNRYVGADRVSDANQVSVGLTSRLLDAQSGRQF
                     IAGTFGQTYYFEIPRVTLPGETPITAGHSDWVAQLSLAAFEDWSVDAGVQWDPQNQRS
                     ERSTVNVQYKPAPNALINLAYRYERFTTTTELVPQVVDGGEQLVATPVQQGYDQVEFS
                     AAWPIKRSWEVFLREVYSLRDPQQPHGQELERFIGFEYRACCWRVRLGARRYVNSHDP
                     TAGQSTGVWLQLELAGLAGVGSASDSFLSEEIRGYAPTAAANVKAQGPLKAIW"
BASE COUNT          397 a          925 c          926 g          477 t
ORIGIN      
        1 ggctacctcg cacagcaaat cggtgctgat ggcatcgctc accgcgcgca gcggcaggtg
       61 ggtggtggcg agcgcgacgc gcagcgctcc ggcaacgagc agcatcaccg cgcgctgcgc
      121 gtgcgtgcgc cgctgcaggt attcggtgtg gccgctgaag acgatgccgg cgtcgttgat
      181 cacgcctttg tgcacgggtg cggtcaccag cgcgtgaaac tccccggcga gcgtgccctc
      241 gatggcccga tcgaggagcg ccagcacgta gcgtgcgttg gcgggatctg gccgtccggc
      301 gatgcttggc gcggcgagcg gcacgtgcgc cacgcagagc tcgccgcgcc gctccgccgc
      361 ggcttgcggc ccggcggcac ccgggtagtc acacaggcgc acagcgagcc gcagctgagc
      421 cgcgcgttgc tcgagcagca tccgatcgcc gaggcacacg agctcacagg gcagctgccg
      481 cgttgccagc gcgaggcaca gctccgggcc gatccccgcc ggctccccac tggtgagcgc
      541 gatacgggga atcaccgcgc gtcgctatga cttgtattcg acgtacgctt cgtcccgcat
      601 gcgccgcagc cacaactcgg tttcctcgtc ggccttgctg gcgcggatgg cttccatggc
      661 ctggcggcgc ttgatctcgt cggagttgtc gaagcgacgg tggccgagca tctgtgcgat
      721 gtgccagccg aactgcgagc ggaagggctc gctgatctcg ccgtccttga gcgtcgcgat
      781 cgcctgctcg aattccggcg caaaggtgcc ggggccgctc cagccgagat cgccgccctc
      841 ggcggccgag cccggatcct gcgaggtgat ctgggcgatc gcggcgaaat cctcaccctt
      901 gagaatgcgc tcgcgcagcg cctcgagctt gccgcgcacg gtggcatcgt cggcgagctc
      961 gttggtcttc atgagaatgt gccgcacgtg tacctggtcc tccacggcgt tctcggtgcc
     1021 gccgcgtatc tcgttgaggc ggatgatgtg atagccggtc ggggtgcgca gcggctcact
     1081 gacctcgccc ggtttgagtt tggcgatgat gtccgccagg aaggtcggca gctcgctgcc
     1141 cttgcgccag ccgagcgcac cgccctcgag cgcggtctgg ctgttggaat aagccaccgc
     1201 gagcttggcg aagtcttcgc cgcctctggc gcgctcgaag acctcctgcg cacgcctggc
     1261 cgcggcttcc tgctgtcccg cggtcgcctc ctgaccgacg gcgatgagga tgtgcgagac
     1321 gttgtactcg ttgcgttccg agggggcctt cgactgccgc tcgaggaact gatcgatctc
     1381 gcgcggcgtg acgctgatgt gttgcagcac atcgcgctga cgcagcatct gcagcatcat
     1441 ctccctgcgg atctcctcgc ggtactccgc gtagtcgccg ccctggcgcg cgagcgcctc
     1501 gggtagctgc gtgagcgtca gaccgttgcg cttcgccaca tcctgcagcg cagagttcag
     1561 catctcatcg gacaccttga ccccggcgtg gttggcgcgc tgcagctgga tctcctgcag
     1621 caccagacgc tcgagtacct gctggcgcag cacgttctgc ggtggcagct cgagtttctg
     1681 ctggcgcagc cgctcgctga cggaaccgat ctgcgcatcg aggtcgctgt tgagcacgac
     1741 gccgtcgttg acgatggcca ccacccggtc gagcagctcc ccctgggtag cgagctcacg
     1801 ggtctgcgcc tgcgcgccgc cgtgcaccgc cagggcgccg agccccagca ccatccacat
     1861 cgggatcgaa gagatcgcag tctgccgcat ggtttttcga acgtccctca ccagatggcc
     1921 ttcagcgggc cctgggcctt gacattggct gccgccgtgg gcgcatagcc gcggatctcc
     1981 tcactgagga agctatccga cgccgatccg acaccggcca gtccggcgag ttccagctgc
     2041 agccacacac cggtgctctg gccggcggtt gggtcgtggc tattgacgta ccgccgcgcc
     2101 cccaggcgga cacgccagca gcatgcacgg tactcgaagc cgatgaaacg ctccagttcc
     2161 tgaccgtgcg gctgctgggg gtcacggagc gagtagacct ccctgaggaa cacctcccag
     2221 ctgcgcttga tgggccaggc ggcggagaac tccacctggt cgtacccttg ctgcaccggg
     2281 gttgcgacca gctgctcgcc gccgtcgacc acctgcggga ccagctcggt cgtcgtggtg
     2341 aagcgctcgt agcggtatgc caggttgatg agcgcgttgg gagcgggctt gtactgcacg
     2401 ttgaccgtgg agcgctcgct gcgctggttc tgggggtccc actgcacccc cgcatcgacg
     2461 ctccagtcct cgaaggccgc gagcgacagc tgcgccaccc agtcggagtg ccccgcggta
     2521 atcggggtct ccccgggaag cgtcacgcgc ggaatctcga aatagtaggt ctggccgaag
     2581 gtgccggcga tgaactgccg gccgctctgg gcatcgagca ggcggctcgt gagcccgacg
     2641 ctcacctggt tggcatcgct cacgcggtcg gcgccgacgt agcggttggt acgaaacagc
     2701 tgcacgggat tgaggtcggg gatcg
//
LOCUS       DATDMP010000365         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_170361, whole genome shotgun sequence.
ACCESSION   DATDMP010000365 DATDMP010000000
VERSION     DATDMP010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170361"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VI195_06410"
     CDS             complement(<1..607)
                     /locus_tag="VI195_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6824055.1"
                     /translation="MKHAGLNTLCVHAAHAPDAVTGAVAEPIHLSTTFERDADGEYPR
                     GYRYSREGTPNRTALESCLAQLEGGAGAAAFASGLAANMAVLELLSAGDLLVASHEGY
                     YGSLRQFRVFAERRGARIELVDMADPATAEAALARRGVRARLLWIETPTNPQLSVVDL
                     RALTALGHEAGALVVCDNTFATPICQRPFEFGVDLIVHSATK"
     gene            671..1618
                     /locus_tag="VI195_06415"
     CDS             671..1618
                     /locus_tag="VI195_06415"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY6824056.1"
                     /translation="MQLQHDEWPELPYEAWRDTVATLHLWTQIAGKVRLSLTPWVNHS
                     WQVPLYVSARGLTTSPIPYQGEIFELEFDFSRHRLVARSSRAEERSFALEPQTVADFY
                     AQLRGLLHALGVVVAINERPSELADPIPFPDDRIHAAYDAAAAQRFWRALIKTDTVLK
                     LFRSGFLGKVSPVHFFWGSFDLAVTRFSGRPAPPHPGGVPGLADAVTREAYSHEVSSA
                     GFWPGNDRFPHAAFYSYAYPEPAGFGAAPLSPGARYEAALGEFVLPYETVRRSADPQG
                     MLLDFLTRTYEAAAECGHWDRKALECALGAPGRVRPVAG"
     gene            1629..>2011
                     /locus_tag="VI195_06420"
     CDS             1629..>2011
                     /locus_tag="VI195_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6824057.1"
                     /translation="MKSLGRNPEGARLERLRASPRWAGDGFRNLHPILPGLRDPHTPP
                     PTLREFLCKSDRRVPPAPLPALDPLEAWHRPPGSGLRVTWLGHSTVLIEIDGLTVLTD
                     PVWGLRASPLRVAGPRRFQPVPVPLR"
BASE COUNT          290 a          678 c          707 g          336 t
ORIGIN      
        1 acttggtggc gctgtgcacg atcaggtcga cgccgaactc gaaaggtcgc tgacagatgg
       61 gggtcgcaaa ggtgttgtcg cacaccacga gcgcaccggc ctcgtgaccg agcgcggtga
      121 gtgcccgcag gtccacgaca ctgagctgcg gattggtcgg tgtctcgatc cacaggagcc
      181 gcgcgcgcac gccgcggcga gcgagtgccg cctcggcagt cgcgggatcg gccatgtcga
      241 ccaactcgat gcgggccccg cgacgctcgg caaacacgcg aaactgccgc agcgagccgt
      301 agtagccttc gtgcgaggcc acgaggagat cgccggcgct gagcagctcc agcaccgcca
      361 tgttggccgc gaggcccgag gcgaacgcgg ccgcgccggc gccgccctcg agctgcgcca
      421 ggcagctctc gagcgcggtg cggttggggg tgccttcgcg gctatagcgg tagcctcgcg
      481 ggtactcgcc gtccgcatca cgctcgaagg tcgtgctgag gtggatcggc tccgccactg
      541 cgccggttac ggcatcggga gcgtgtgcgg cgtgaacgca gagcgtattg agaccggcgt
      601 gcttcatcgg gtcgagctta cactatcggt gcggcggctc cgcgggagcg ccgttggacg
      661 aggagcgccg atgcagctgc agcacgatga gtggccggaa cttccttacg aggcgtggcg
      721 tgacacggtg gcgacactgc atctgtggac gcagatcgcc ggcaaggtga ggttgtcgct
      781 gacaccgtgg gtgaatcaca gctggcaggt gccgctgtac gtgagcgcgc gcgggctcac
      841 gacctcaccc attccgtacc agggcgagat cttcgagctc gagttcgatt tcagccgcca
      901 ccgtctggtg gcacgctcga gccgcgctga ggagcgcagc ttcgcgctcg agccgcagac
      961 ggttgcggac ttctacgcgc agctgcgcgg gctgctgcac gcgctcggag tcgtggtggc
     1021 gatcaacgag cgcccgtccg agctcgccga cccgatccct tttccggatg accggattca
     1081 cgccgcttat gacgccgctg ccgcgcagcg cttctggcgt gcgctgatca agaccgacac
     1141 cgtgctcaag ctttttcgca gcggctttct cggcaaggtg agcccggtgc attttttctg
     1201 gggcagtttc gatctggccg ttacccgctt ctccgggcgt cccgcaccac cgcatcccgg
     1261 tggtgtgccc ggcctcgcgg atgcggtgac gcgcgaagcg tactcgcacg aggtgagcag
     1321 tgccgggttc tggccgggca acgacaggtt tccgcacgct gcgttctatt cctatgcgta
     1381 tcccgagccc gcgggcttcg gtgcagcgcc gctgagcccg ggcgcccgct acgaggcggc
     1441 actcggcgag ttcgtcctgc cgtacgagac ggtgcggcga tcggcggacc cgcaagggat
     1501 gctgctggac ttcctgacac gcacctacga ggcggcggcg gagtgcggac actgggaccg
     1561 caaagcgctc gagtgtgcgc tcggcgcgcc tggacgggtt cggccggtcg cggggtgact
     1621 cacgtttcat gaaatctctg ggcagaaacc ctgagggcgc gaggctcgag cgtctgcgcg
     1681 catcgccgcg ctgggcaggc gatggctttc gcaacctgca cccgatcctt cccgggctgc
     1741 gcgatccgca cacaccaccg ccaactctgc gcgagttcct ctgcaagagc gaccggcgcg
     1801 tgccgccggc gccgctgcct gcgctcgacc cgctcgaggc atggcaccgc cctccgggca
     1861 gcggcctgcg cgtcacctgg ctcggtcact cgacggtgct catcgagatc gatggcctca
     1921 cggtgctcac cgaccccgtg tgggggctgc gtgcatcacc gctgcgcgtc gccggtccgc
     1981 ggcgcttcca gccggtgccg gtgccgctgc g
//
LOCUS       DATDMP010000366         3412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2170509, whole genome shotgun sequence.
ACCESSION   DATDMP010000366 DATDMP010000000
VERSION     DATDMP010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170509"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      2..75
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            181..1095
                     /locus_tag="VI195_06425"
     CDS             181..1095
                     /locus_tag="VI195_06425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY6824058.1"
                     /translation="MNVILLSKREGRARQLDLARPLTLTVLAALVVVFLGSAFALGLQ
                     LGRGNHERVVLSDTVQFGALLTEQKKQIAELKQQLQLRVDAMAMRLGEVNAHVIRLDA
                     LGKRLTEMADIDSREFNFDRDPPSGGPEGEGRSAQIPDLSAMLAQLEQRVDQRESQLA
                     ALENLILARELHEEIHPEGRPVANGFISSYFGERSDPFDGRDAFHKGVDFAGALGSNV
                     TAVAAGVVTWAGERSGFGKLIEINHGDGFVTRYAHNERTLVTVGETVKRGEPIALMGS
                     SGRSTGPHVHFEVLRNGRQVDPLSFIGR"
     gene            1268..>3412
                     /gene="secA"
                     /locus_tag="VI195_06430"
     CDS             1268..>3412
                     /gene="secA"
                     /locus_tag="VI195_06430"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY6824059.1"
                     /translation="MRQLLTRVFGSRNDRIDRGHGRAVRAANQLEPQITELSDEALRA
                     KTEEFRQRLKAGAALDELLPEAFAVVREAARRTLKMRHFDVQLIGGITLHKGMIAEMR
                     TGEGKTLVATLPAYLNALSGEGVHIVTVNEYLAQRDADWMGPVYRFLGLTVGVIKNAQ
                     TAQEKRAAYACDITYGTNNEFGFDYLRDNLAYRLEERVQRALSFAIVDEVDSILIDEA
                     RTPLIISGPAEESTELYLRINQLVPRLTRQKQEDGPGDFSVDEKTKQVHITEAGHENV
                     EQLMLQSELLKEGESLYDPANIRLMHHLNAALRAHALYKRDVEYIVRAGEVIIVDEFT
                     GRTMPGRRWSDGLHQAIEAKEGVRVREENQTVASITFQNYFRLYKKLSGMTGTADTEA
                     PEFLQIYGLEVVVIPTHRPMIRKDNPDFVYLTQSDKFKAIIEDIRDCVTRAQPVLVGT
                     TSIETSEFLAGLLQKEAVSHQVLNAKQHEREAHIVAQAGRPGTVTIATNMAGRGTDIV
                     LGGNLEAELAAAGELDEAARERLRGDWQARHDEVVAAGGLHIVGTERHESRRIDNQLR
                     GRSGRQGDPGSSRFYLSMEDNLMRIFGDPVRTQRLLKMAGMKEGEVIESGMLTRQIEK
                     AQRKVESHNFDIRKNLLLFDDVANDQRKVVYAQRTEIMGTEDLSNAIRGLMEDSVGTL
                     LDQHLPRQASPEDWDLEGLRTAVQRDFNTSIDP"
BASE COUNT          650 a         1127 c         1116 g          519 t
ORIGIN      
        1 gggcaaaccc gccgaaaggc ggggacgcaa agccaccggt ctaaagcgag gagccatgac
       61 agcggggttg ccagcatcgg gagggtataa gcaaaaaaag ggcgacgccg cagcggtgct
      121 tgtccatgcg cccccagagg gcgcggcctg gcggggacat aattcggtgg caagcgggat
      181 atgaacgtca ttttgctatc caagcgtgag gggagggccc ggcagctcga tctcgcgcgc
      241 ccgcttacgt taactgtcct cgccgccctg gtggtcgtgt tcctcggcag cgcttttgcg
      301 cttggcttgc agctcggccg cggtaaccac gagcgcgtgg tgctgagcga tacggtgcaa
      361 tttggcgcac tgctcaccga gcagaaaaag cagatcgccg agctcaaaca gcagctgcag
      421 ctgcgtgtcg acgcgatggc catgcgcctc ggcgaggtca acgcgcacgt gatccggctc
      481 gatgcgctcg gcaagcgcct caccgagatg gccgacatcg acagccgcga gttcaatttc
      541 gaccgcgacc cgccgagcgg cggtccggaa ggcgagggta ggagcgccca gattcccgat
      601 ctatccgcca tgctcgcgca gctcgagcag cgcgtggacc agcgtgaatc gcagctcgcg
      661 gcgctcgaga acctgatcct ggcgcgcgag ctgcacgagg agatccaccc cgagggccgc
      721 ccggtcgcca acggcttcat ctcctcctac ttcggcgagc gctccgatcc cttcgatggc
      781 cgcgatgcct tccacaaggg cgtggacttc gccggcgcgc tcgggtcgaa cgtcaccgca
      841 gtggccgccg gggtggtgac ctgggcgggc gagcgctctg gcttcggcaa gctcatcgag
      901 atcaatcacg gcgacggctt cgtcacgcgc tacgcccaca acgaacgcac cctggtgacg
      961 gtcggtgaga ccgtgaaacg cggtgagccg atcgccctca tgggatcgag cggccgctcg
     1021 accgggccgc acgtgcactt cgaagtgctg cgcaacggcc gccaggtcga ccccctctcc
     1081 ttcatcgggc gctgagcaaa gccccgctgc gcgaggcggc gccgccgcga gcgcgcaggc
     1141 tcgagcggcc cccgcgaacc aattgggcta ggcgggcgtc ttatgcttac aatacgccgc
     1201 tttcgaatgc ccgcctgtac ggtaagtgct tgccgggaaa gatgttttcc cccaagcgag
     1261 tcacaccttg cgccagcttc tcacccgcgt tttcggcagc cgcaatgacc ggatcgaccg
     1321 cggccacggc cgcgcggtgc gcgccgccaa tcagctcgag ccgcagatca ccgagctgag
     1381 cgatgaggcg ctgcgcgcga aaaccgaaga gttccgccag cggctgaagg cgggcgcggc
     1441 gctcgatgag ctgctgcccg aagccttcgc agtcgtgcgc gaggccgccc ggcggacgct
     1501 caagatgcgc catttcgatg tgcagctcat cggcgggatc acgctccaca agggcatgat
     1561 tgccgagatg cgcacgggcg agggcaagac gctggtcgcg acgctgcccg cctacctcaa
     1621 tgccctgagc ggcgagggcg tgcacatcgt gaccgtgaac gagtacctgg cgcagcgcga
     1681 tgcggactgg atgggtccgg tgtaccgctt cctcggtctc acggtcgggg tcatcaaaaa
     1741 cgcgcagacc gcgcaggaga agcgtgcggc ttacgcctgc gacatcacct acggcaccaa
     1801 caacgagttc ggcttcgatt acctgcgcga caatctcgcc taccggctcg aggagcgcgt
     1861 gcagcgcgcc ctgtcctttg ccatcgtgga cgaggtcgat tccatcctca tcgacgaagc
     1921 gcgcacgccg ctgatcattt ccggtcccgc ggaagagagc accgagctct acctgcgtat
     1981 caaccagctg gtgccgcgtc tcacgcggca gaagcaggag gacggtcccg gggacttctc
     2041 cgtggacgag aagaccaagc aggtccacat caccgaagcc ggccacgaga acgtcgagca
     2101 gctcatgctg cagagcgagc tgctcaagga gggcgagagc ctgtacgacc cggccaatat
     2161 ccgcctgatg catcacctca acgcggcgct gcgggcgcat gcgctctaca agcgcgacgt
     2221 cgagtacatc gtgcgcgccg gcgaagtgat catcgtggat gagttcaccg ggcgcaccat
     2281 gcccgggcgg cgctggtcgg atggactgca ccaggcgatc gaggccaaag agggcgtgag
     2341 ggtgcgcgag gagaaccaga cggtggcctc gatcactttc cagaactact tccgtctcta
     2401 caagaagctc tccggcatga ccggcaccgc ggacaccgag gcacccgagt tcctgcagat
     2461 ctatgggctg gaagtggtgg tcattcccac tcatcgtccg atgatccgca aggacaatcc
     2521 ggacttcgtc tatctgactc agagcgacaa gttcaaggcc atcatcgagg acatccgcga
     2581 ttgcgtgacc cgtgcgcagc cggtgctggt gggcaccacc tcgatcgaga cctcggagtt
     2641 cctcgcgggc ctgctgcaga aggaagccgt cagccaccag gtgctcaacg ccaagcagca
     2701 cgagcgcgag gcacacatcg tcgctcaggc cggccgcccc ggtaccgtca ccatcgccac
     2761 caacatggcc ggccgcggca ccgacatcgt gctcggcggc aatctcgagg ccgagctcgc
     2821 cgcagccggc gagctcgatg aggcggcgcg cgagcgactg cgcggcgact ggcaggcgcg
     2881 ccacgacgag gtggtcgcgg ccggcggcct gcacatcgtc ggcaccgagc gtcatgagtc
     2941 gcgccgcatc gacaaccagc tgcgcggccg ttccggaaga cagggtgatc cgggctcgag
     3001 ccgcttctac ctgtcgatgg aagacaacct gatgcgcatc ttcggcgacc cggtgcgcac
     3061 gcagcgcctg ctgaagatgg ccgggatgaa ggaaggggag gtcatcgaga gtggtatgct
     3121 cacccgccag atcgagaagg cgcagcgcaa ggtcgagtca cacaacttcg acatccgcaa
     3181 gaacctgctg ctgttcgatg acgtcgccaa cgatcagcgc aaggtcgtgt acgcgcagcg
     3241 caccgagatc atgggcaccg aggatctctc caacgccatt cgcggactga tggaggactc
     3301 ggtcggcacg ctgctcgatc agcacctgcc gcgccaggcc tcgccggagg actgggatct
     3361 cgaaggcctg cgtaccgcag tgcagaggga tttcaacacc agcatcgacc cc
//
LOCUS       DATDMP010000367         2667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1281813, whole genome shotgun sequence.
ACCESSION   DATDMP010000367 DATDMP010000000
VERSION     DATDMP010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281813"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VI195_06435"
     CDS             complement(<1..902)
                     /locus_tag="VI195_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY6824060.1"
                     /translation="MPEVASQTFELVGREVNATLAAARSALESYVEQPDNSSLLEQCA
                     QDLHQVQGVLRMLEIYGAALLAEEMEHVAQYLLSTHSERKSQAESLDALMRAMVQLPS
                     YLERVLAGGRDLALVLLPLLNDLRAVRGHALLSEGTLLLLNLKSDKQAQPVAATAGEP
                     PLTVEQWARRLRARFQTGLIGWIRGERIAQNLEILSAVARKLEQIATRQPVFQLWWVV
                     GAVIESLQENGLESGQSVKRLLGLADREIRRLYEQGEARYAQSPAVELLNNLLYYVGR
                     AESGGGRVNAVRTSFRLAELLPVD"
     gene            complement(924..>2667)
                     /locus_tag="VI195_06440"
     CDS             complement(924..>2667)
                     /locus_tag="VI195_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6824061.1"
                     /translation="LRARAALTDAAAAARDTTTLVPRLTAAARVLEASLPPSAAAAAA
                     GTFERFEARAQRLQLDVTALSQGSSDPAQAAQRIAESSEYLGQVIAGFAGGSTALALP
                     RVTTPDSAKHLKTLDSIYSELAGAVKRAVGAAPALPAAQTAARAIVADARDLAAGAAR
                     SAAPAASARALRWLPLVLLALGLALLLAALMAALRVHRTLERQRASAETQRRESDRNQ
                     QAILRLLDELSSLADGDLTVQATVTEDITGAIADSINYAVDALRGLVTTINASAIQLD
                     SATRQTQALSQHLAKASGAQSKQIASATESAASMAASVEEVSGNAERAADVARHSVEV
                     AHKGGDAVRRTIDGMNTIRETIQETSKRIKRLGESSQEIGNIVELINDIAEQTNILAL
                     NASIQSSMAGEAGRGFAVVADEVQRLAERAANATKQIEVLVRTIQTDTNEAVVSMERS
                     TTDVVGGALLAENAGAALGEIEQVSNQIASLVQNISGSSRQQTGAAQNIARNMKVLKE
                     ISAQTAESTQATSIAIAKLAELSAGLRKSAAGFRLPGGAAADRAGSSGRVRRLEDPML
                     NTAKVRQIATLGGS"
BASE COUNT          351 a          919 c          962 g          435 t
ORIGIN      
        1 tcatcgaccg gcagcagctc cgcgagccgg aacgaggtgc gcaccgcgtt cacgcgtccg
       61 cccccggatt cggcgcggcc gacgtaatag agcagattgt tgagcagctc caccgctgga
      121 gactgcgcgt agcgcgcctc gccctgctcg tacagccggc ggatctcgcg atcggcgagg
      181 ccgagcaggc gcttcaccga ctgtccgctc tcgagcccgt tctcctgcag cgactcgatg
      241 accgcaccca ccacccacca cagctggaac accggctgac gggtggcgat ctgctcgagc
      301 ttgcgcgcca cggcggagag gatctcgagg ttctgtgcga tgcgctcgcc gcggatccag
      361 ccgatgagtc cggtctgaaa gcgcgcgcgc agccgccgcg cccactgctc gaccgtcagc
      421 ggtggctcgc ccgctgtggc cgccacgggc tgcgcctgct tgtccgactt gaggttcagc
      481 agcagcagcg tgccttcgct gagcagggcg tggccgcgca ccgcgcgcag atcgttcagc
      541 agcggcagta gcacgagagc cagatcgcgc ccgccggcga gcacgcgctc gagataggag
      601 ggcagctgca ccatggcgcg catcagcgca tcgaggctct ccgcctggct cttgcgctcg
      661 gaatgggtcg atagcaggta ctgcgcgacg tgctccattt cctcggcgag cagcgcggcg
      721 ccgtagatct ccagcatgcg cagcacaccc tgcacctggt gcaggtcctg tgcgcactgc
      781 tcgagtaagc tggagttgtc gggctgctct acgtagctct cgagcgcgct gcgcgcagcc
      841 gcgagcgtcg cgttcacctc gcggccaacg agctcgaatg tctgggaagc gacttcaggc
      901 atgccgtgct ttcgctgcgc gcgtcaggag ccgccgaggg tggcgatctg ccgtaccttg
      961 gcggtgttga gcatggggtc ctcgagtcgc cgcactcggc cgctcgagcc ggcccggtcg
     1021 gcggccgcgc cgccgggcag gcggaagccg gcggcggatt tgcgcagccc ggccgagagt
     1081 tccgcgagct tggcgatcgc gatggaggtc gcctgggtcg actcggcggt ctgcgcgctg
     1141 atctccttga gtactttcat gttgcgggcg atgttctgcg ccgctccggt ctgctggcgc
     1201 gagctgccgg agatgttctg caccagactc gcgatctggt tggacacctg ctcgatctcc
     1261 ccgagcgcgg cgccggcatt ctcggcgagc agtgcgccgc cgaccacatc ggtggtgctg
     1321 cgctccatcg ataccaccgc ctcgttggta tcggtctgaa tggtgcgcac cagcacctcg
     1381 atctgcttgg tggcattcgc ggcacgctcg gcgaggcgct gcacctcgtc cgccaccacc
     1441 gcgaagccgc ggccggcctc gccggccatg gaggactgga tggaggcgtt gagcgcgagg
     1501 atgttggtct gctcggcgat gtcgttgatc agctcgacga tgttaccgat ctcctgcgag
     1561 ctctcgccga ggcgcttgat gcgcttgctg gtctcctgga tggtctcgcg gatggtgttc
     1621 atgccgtcga tggttcggcg caccgcatcg ccgcccttgt gcgccacctc caccgagtgt
     1681 cgcgcgacgt cggcggcacg ctcggcgtta ccggagactt cctccaccga ggcggccatg
     1741 ctggcggcgg attcggtcgc ggaagcgatc tgcttcgact gcgcgccgga ggccttggcg
     1801 agatgctgcg acagcgcctg cgtctggcgg gtggcggaat ccagttggat cgccgatgca
     1861 ttgatggtgg tgacgaggcc gcgcagggca tcgaccgcgt agttgatcga gtccgcgatg
     1921 gcgccggtga tgtcctcggt caccgtggcc tgcaccgtga gatcgccatc ggcgaggctc
     1981 gagagctcat cgagcagccg caggatcgcc tgctggttgc ggtcgctctc gcggcgctgc
     2041 gtttccgcgg aggcgcgctg ccgctcgagc gtgcgatgca cccgcagcgc ggccatgagc
     2101 gccgccagca gcagtgcgag tcccagcgcg agcagcacga gcggcagcca ccgcagcgcg
     2161 cgcgcactcg ctgccggcgc ggccgagcgc gccgcgcccg ccgccaggtc gcgcgcgtcc
     2221 gcgacgatgg cgcgcgccgc cgtctgcgcg gcaggtagcg ccggggcggc gccgacggcg
     2281 cgcttcacgg ccccggcgag ctcgctgtag atcgaatcga gggtcttcag atgcttggcg
     2341 gaatcgggcg tagtgacgcg cggcagcgcg agcgccgtgg agccgccggc gaagcccgcg
     2401 atcacctgcc cgaggtactc gctgctctcg gcaatgcgct gtgcggcctg ggcggggtcg
     2461 ctgctcccct gcgagagcgc ggtcacatcg agctgcaggc gctgcgcgcg cgcctcgaat
     2521 cgctcgaagg tgccggcggc cgcggccgct gccgaaggcg gcaggctcgc ctcgagcacg
     2581 cgcgccgccg cggtgagacg cggcacgagc gtcgtggtgt cgcgcgccgc tgcggcggcg
     2641 tcggtgagtg cggcgcgcgc gcgcagc
//
LOCUS       DATDMP010000368         3005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1948339, whole genome shotgun sequence.
ACCESSION   DATDMP010000368 DATDMP010000000
VERSION     DATDMP010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948339"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VI195_06445"
     CDS             complement(<1..771)
                     /locus_tag="VI195_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6824062.1"
                     /translation="MRRLTNEELKAENARRLLHPMAHPGTVRDALPDIIARGEGSWIW
                     DVDGHRLVDGIGGLWCTNLGFGRREIRDAIVAQLDELPFYNTFRGATHPRAIELSQRL
                     VTLMAPDGVGAVHFSNGGSDAVEGALKIARQYWKVQGQRDRTKFISLRKGYHGTHFGG
                     ASVNGNTPFRRAYEPLLPGCFHVDTPWLYRNPYTQDPEQLGRICAELLEREIVFQGAD
                     TVAAFIAEPIQGAGGVIVPPPNYWPLVRAVCDRHGVLLI"
     gene            1117..2810
                     /locus_tag="VI195_06450"
                     /pseudo
     CDS             1117..2810
                     /locus_tag="VI195_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
     gene            complement(2823..>3005)
                     /locus_tag="VI195_06455"
     CDS             complement(2823..>3005)
                     /locus_tag="VI195_06455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6824063.1"
                     /translation="LTAQRIGHALIHAATERGRRDGKLRMTLSTAKTNASAQSLYESL
                     GWERDEEFYVYNLGMV"
BASE COUNT          529 a         1023 c          947 g          506 t
ORIGIN      
        1 gatgagcagc acgccgtgcc ggtcgcagac cgcgcgcacc agcggccagt agtttggcgg
       61 cggtacgatc accccgcccg ccccctgtat cggctcggcg atgaacgccg cgaccgtgtc
      121 cgctccctgg aagacgatct cccgctcgag aagctcggcg cagatgcggc cgagctgctc
      181 cggatcctgc gtataggggt tgcggtacag ccacggcgtg tcgacgtgga agcacccggg
      241 cagcagcggc tcgtaggcgc gacggaaagg ggtgttgcca ttcacactcg cgccgccgaa
      301 gtgcgtgccg tgatagccct tgcggagcga gatgaacttg gtgcggtcac gctggccctg
      361 caccttccag tactggcgcg cgatcttgag ggcaccctcc accgcatccg agccgccgtt
      421 gctgaagtgc accgcaccga cgccatcggg cgccatgagc gtcaccaacc gctgcgagag
      481 ctcgatcgcg cgaggatgag tggcgccgcg gaaggtgttg tagaaaggaa gctcatcgag
      541 ctgcgcgacg atcgcatcgc ggatctcgcg gcggccgaag ccgaggttcg tgcaccacag
      601 gccgccgatg ccatcgacga ggcgatgccc gtcgacgtcc cagatccagc tgccctcgcc
      661 gcgcgcgatg atgtcgggga gcgcatcgcg caccgtgccc ggatgagcca tcggatgcag
      721 caggcgccgg gcgttctccg ccttgagttc ctcgttcgtt agtcttcgca tgagccgtaa
      781 atccctggaa tggcgtaacg agtgtaactc acgccgcatg gctcacgcct tgaacataac
      841 ctcgctgcgc ggctacaccg gcactccttt ataaagagtg gcggacactg gtggcgcgcg
      901 catcttaacc accagaaatc aaagccttgg gttctcaggc cctgtgtaga atccgtcaaa
      961 gccttagccg cccaggggga gcagcggatg agagcactcg ccggttcact gttgatcgtg
     1021 gtctccacgg caagctccgc ggcgctcttt cccacgagtc cttcggatct cagcgctgcg
     1081 gagagcgtgc agctttcctg ggcggtgaag atccccatgc gcgatggcgt gcggttgaat
     1141 gcgaccgtct acaccccgcg cgctcagccg gcgccggccc cctgcatttt cacactcact
     1201 ccctacatcg gacagagcta tcacgcgcgc ggcgtctact ttgcagcgca cggcctcccc
     1261 tttctcacgg tcgacgtgcg cggtcgggga aattcagagg gcgaattccg gcccttcata
     1321 caggaggcga gcgacggcta cgacgtgacc gagtggctcg cgcggcagcc ctggtgcaac
     1381 ggcaaggtgg ccatgtgggg cggctcctac gccggctatg accagtgggc cacggcgaag
     1441 ggctcccgcc tcaccttgcg acgatcgttc ccgcggcctc ggcctacgcg ggtgtcgatt
     1501 ttccgatgac cgccaacatc ttcgcgccct acgacatgca gtggctgacg ctcaccagcg
     1561 gccgcaccgg gcagcaggca ctcttcgagg acaatccttt ctgggcggcg aagtaccggc
     1621 agtggttcac ggccggcgca ccgtacgcgg gcctcgatac gctgatcggc aatccttcca
     1681 aggtcttcca ggaatggatt cagcacccgc atcccgatgc gtactgggat gcctataacc
     1741 cgacggccga gcagtattcg aagcttgcaa tcccgatcct caccattacc ggaagtcacg
     1801 atgacgacca gccgggcgcc ctcacgcact accgcgagta catgaagcat gcatcgcccg
     1861 agggacgtgc ccgccattac ctgatcatcg gcccctggga ccatgccggc acgcgcacgc
     1921 cgagcgcgca gttcggcggc ctcagcttcg gtcccgcgag cctcctcgac ctgccgaaac
     1981 tccatctcga gtggtacgcc tggagtatgc agggcggcgc gaagcccgct ttcctgcgca
     2041 agccggtcgc ctactacgtg atgtacgcgg accgctggcg ttacgcggac acgctcgagg
     2101 cggtcacggc cggcacgctg gcgctgcatc tggattctgc ggccaatgcg gatgatgtgc
     2161 tcgccgccgg cgcgctcgcg gcgggcgagg cgcgtggacg gcccgaccac taccgctacg
     2221 atccgcacga cgtgagcacc gccgacctgg agtcgaccac ggaccccgcg agcctcgtgg
     2281 atcagcgcat gacctatgcg ctgcgcggta agcagctcgt ctaccacagt gcaccgctcg
     2341 acaaggacac cgaggtgagt ggcttctgca agctgtcggc gtggatcgcc atcgatcagc
     2401 cggacacgga tttcagcgtc agcctctacg agatccgcga ggacggcagc gccattctga
     2461 tgacgagcga tgccctgcgt gcccgctacc gcgagagcgc gcgcgcgccg aaactgatcc
     2521 gcagcaaagc cccgctgcgc tacgacttcg agcgcttcac cttcgtgtcg caggaagtga
     2581 agaagggaag ccgcctgcgg ctcatcgtcg gtcccatcaa ttcgatctac cgcgagaaga
     2641 actacaattc aggcgggttg gtgcagtcgg agtccatggc ggatgcacgc ccggtgagcg
     2701 tcacgctcta tcacgaccgc gagcatccga gcacgctttc cataccgctc ggggcggcgc
     2761 gcgccgcgga cgagccacag gcgccggcct ccgccttcag cgcaccatag ccctaaccgg
     2821 cgctatacca tgcccaggtt gtagacgtag aactcctcat cgcgctccca gccgagcgac
     2881 tcgtagagcg actgcgcgct cgcattggtc tttgcggtgg agagtgtcat gcgcagcttg
     2941 ccatcgcgcc ggccgcgctc ggtcgccgcg tggatcagcg catgacctat gcgctgcgcg
     3001 gtaag
//
LOCUS       DATDMP010000369         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_837555, whole genome shotgun sequence.
ACCESSION   DATDMP010000369 DATDMP010000000
VERSION     DATDMP010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837555"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="VI195_06460"
     CDS             <1..1465
                     /locus_tag="VI195_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6824064.1"
                     /translation="TGARGSTREPWGTDLQTFRAHAVSQHPPQNGRGNISSMEAMMTD
                     NHNSTAGAFQLDVDLRRAGYAADASQTIIEALVRLRAPGKPPHAKPREPITLALVLDR
                     SGSMAGAPLAEAKRCARMIVQSLGPRDRAAVVAFDDEVERLSPVEGSDQRSGLLAAID
                     SIDSGGATDLYGGWLEGARVLGQHVAGTGVHRVILLSDGAANRGEMDLETISASCRDL
                     AKQGISTSTYGLGAAFNEELMLAMAKAGRGNAYYGQTAQDLALPFQDEFALLTSLCAR
                     GMMLKVTASKGVQVALRNDYEPAEGEGTAWKLPDLAFEAEAWAYLKFVVPNVYLTQAS
                     VSLPITVSIKAAGSGSTPLFFMASLPPVQRMSRATLMSMPPHPIVEARREELGAGESL
                     DLVRRLIARGRWDAAASAVDKAQRRFAANPWCKGILDTMQRMIARRDGFGAKEAAYAS
                     AALRRRLAASNESVSSIDAPNIPAFLRRRPEQGRRLP"
     gene            1575..1850
                     /locus_tag="VI195_06465"
     CDS             1575..1850
                     /locus_tag="VI195_06465"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEY6824065.1"
                     /translation="MQHAYTAHARARMQQRGIKPAAVDCVLEFGTPHHDGRGAVVFVL
                     DKRAQRRLLRHRVLSRPELERIRKFYVVLAGDVVVTTGHRYSRVHRS"
BASE COUNT          337 a          684 c          656 g          335 t
ORIGIN      
        1 taccggcgcc cgtggctcaa cgcgtgagcc atggggcacg gacctgcaaa ccttccgggc
       61 tcacgccgtg agccagcacc ctccgcagaa tggtcgaggc aacatctcat ccatggaggc
      121 catgatgacg gacaatcaca acagcaccgc aggcgcattc cagctcgacg tcgatcttcg
      181 ccgcgcgggc tatgccgccg acgcatccca gacgatcatt gaggcgctgg tgcgcctgcg
      241 cgctcccgga aaaccgccgc acgccaagcc gcgtgagccg atcaccctcg cgctggtgct
      301 cgatcgctcc ggttcgatgg cgggtgcacc tctggccgaa gcgaagcgct gcgcccgcat
      361 gatcgtgcaa agtctcggcc cgcgcgatcg cgccgccgtg gtggccttcg acgacgaggt
      421 cgagcgcctg tcgcccgtcg aaggcagcga tcagcgcagc ggcctcctcg ccgcgatcga
      481 ttcgatcgat agcggtggcg ccaccgatct gtatggaggc tggctcgaag gtgcccgcgt
      541 gctgggccag cacgtcgcgg gcaccggcgt ccatcgcgtc attctcctgt ccgatggcgc
      601 tgccaatcgt ggcgagatgg acctcgagac gatcagcgca tcgtgccgcg atctcgccaa
      661 gcagggcatc tccacctcga cctacggctt gggcgccgcg ttcaacgagg agctgatgct
      721 cgccatggcc aaggcgggcc gcggcaacgc gtattacggc cagacggcac aggatctcgc
      781 cctgcccttt caagacgagt tcgcgctgct cacgagcctt tgcgcgcgcg gcatgatgct
      841 caaggtcact gcgtccaagg gcgtgcaggt cgcgctgcgc aacgactacg agccggccga
      901 aggcgagggc acggcgtgga agcttcccga tctcgcattc gaagccgagg cgtgggcgta
      961 tctcaagttc gtggtgccga acgtgtatct cacgcaggcg agcgtgagcc tgccgatcac
     1021 cgtttccatc aaggccgcag gttcggggag cacgccgctg ttcttcatgg catcgctgcc
     1081 gccggtgcaa cgcatgtcgc gcgcgacgct gatgagcatg ccgccacacc cgatcgtcga
     1141 ggctcgacgc gaggagcttg gcgccggaga atcgctcgac ctcgttcggc gtctcatcgc
     1201 gcgcgggcgc tgggatgccg cggcctccgc cgtcgacaag gcgcaacgcc ggtttgcggc
     1261 gaatccctgg tgcaagggca tcctcgacac gatgcagcgc atgatcgcgc gtcgtgatgg
     1321 cttcggggcc aaggaggcgg cgtacgcgag cgccgcgctg cgccgtcgtc tcgcggcaag
     1381 caacgaaagc gtttcctcga tcgatgcgcc gaacattccg gcgttcctgc gccgcagacc
     1441 ggagcagggc aggcggctgc cgtaacgaac gcattgcgca cgcctgcaca agctgtatgt
     1501 gacacccgcg ggatgggcgc gagtgcttga cgatcgtgaa gcgaaaatgg cagtctgcct
     1561 ttgccggagg agaaatgcag cacgcctaca ccgctcacgc tcgtgccagg atgcagcagc
     1621 gcggcatcaa gcctgcggcc gtcgattgcg ttctcgagtt cggaacgccg catcacgacg
     1681 gccgcggcgc cgtggtgttc gttctcgaca agcgtgcgca gcggcgtctg ttgcgccatc
     1741 gcgtgctgtc gcgccccgag ctcgagcgca tacgcaagtt ctacgtggtg ctcgccggcg
     1801 acgtggtggt cacgacgggt caccgttact cgcgcgtgca tcggagctga ttcgcgcgct
     1861 gctcgcgacg aagcccatcg taaactgacg ggatggatcg caccgagcgc ttctatctca
     1921 tcgagcgcct gatcagccag cgcaagctcg tcactcgcca ccagctcctg cgcgcgctcg
     1981 gcgtctctct cgcaaccctc aagcgcgatc tc
//
LOCUS       DATDMP010000370         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1837438, whole genome shotgun sequence.
ACCESSION   DATDMP010000370 DATDMP010000000
VERSION     DATDMP010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837438"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..643
                     /locus_tag="VI195_06470"
     CDS             299..643
                     /locus_tag="VI195_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6824066.1"
                     /translation="MYTGSCHCGRIAFEVEGNFTEVNECNCSHCSRKGYLLWFVPREQ
                     LRLKCAENSYSTYRFHKHVIAHHFCPTCGCAPFATGKGPGGVATAAINVRCLENIDLA
                     ALKRVPIDGRSR"
     regulatory      832..916
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1128..>2385
                     /locus_tag="VI195_06475"
     CDS             1128..>2385
                     /locus_tag="VI195_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824067.1"
                     /translation="MSMTSLLTFTPWPLLSATILFILLVAALYLARDTAHQAITAAAA
                     ALSRGLRVASHSVIRAQQQLAARNRAVLLSAGREAKERIVEREFARVGDTVRKDLAGY
                     PEMHRRLSEAILRIEEDQAKAVEVPPEAPGWAQAVKVVADLDTRNAGADILADIHKSM
                     VKAHDEAMNDYRKASGERHALLRRMMPDWRLIQDTLGRVNKSVESVIGRALAIDRHME
                     EYEAIVRGEDRAVSILSSSSIVYFFISLLVLAVATAGTAVNFTLIARPMAEMVGGTNF
                     IGAYRTADIAALVIIMVEISMGLFLMESLRITRLFPVIGALSDKMRVRMIGVTFTILL
                     LMASIEAGLAYMREVLLQDELATSALLRGDTSSAGSIVYSHLWITTAAQMGMGFVLPF
                     ALVFVAIPLETFVHSLRTVVGLIGIAA"
BASE COUNT          393 a          811 c          767 g          414 t
ORIGIN      
        1 cttcacgacc acccccggtg ctgtcgcggg cgtacgtcgc atcctctccc agcagaccgt
       61 gcaccaatcg gagggtttcg ccgtccgcgc tcgaggcggc gggggagtgc cgtcttgcgc
      121 ggccgcggca agccgcgatt gtgacgacgg tccgattgtc cccgcagcga gtgagaccgg
      181 ccgcaaattg cgccagcggc tttctggggt gatctccccc caccggcgcc cggcccggcc
      241 tgccccgact tggggccggc ggcttcggac gatagactgc cggcggaggc agactctcat
      301 gtacaccggc agctgccatt gcggtcgcat cgcattcgag gtcgagggga acttcaccga
      361 ggtcaatgaa tgcaactgct cgcactgcag ccgcaagggc tatctcctct ggttcgtccc
      421 gcgcgagcag ctgcgcctca agtgcgccga gaacagttac agcacctacc gcttccacaa
      481 gcacgtcatt gcccaccact tctgcccgac gtgtggctgt gctcccttcg ctaccggtaa
      541 gggtccgggg ggcgtggcga ccgctgcgat caacgtgcgc tgcctggaga acatcgatct
      601 cgcggcgctc aagcgcgtgc cgatcgacgg tcgcagtcgc tgagcgcttc agccacggtg
      661 cgcgtgtgtc cgcgcgctac ggcctgtgtc tcagttcggg gcaggccgac tgagaagcag
      721 ctataagaaa gaatgagagg gcgtcatttg cgcatgacat aagccatgat gccaaacagc
      781 gacaggttga cgcgttgact tgggcaatcc gctaccccac cctaggatca catctgcacg
      841 aggcacacct ttcgaaagaa ggggtcgcaa agccaccggt ctaagggggt caacctatga
      901 cagcggggtt gccagcatgg aaaagcgcac ggcaatggcg cggcctcacg ttccgcccgt
      961 gaatcgagcg cacctcgagc gcggcgcgca tcccactgcg catcggaccc gaccgttcgg
     1021 ctgacgcccg ctctggcgca ctcccactgc tgaccctgcc acgggcgctg gccttgccgc
     1081 ccactgaccg tccctaccca tttttccgat cgcaagcgag cactgacatg tccatgacaa
     1141 gcctgttgac cttcacgcca tggccgctgt tgtcggcgac cattctgttc attctgctgg
     1201 tggcagcgct ctacctggcg agggacacgg cgcaccaggc gatcaccgct gccgccgcgg
     1261 ccctctcgcg cggcctgcgc gtcgcctcgc actcggtgat ccgcgcgcag cagcagctcg
     1321 ccgcgcgcaa ccgcgcggtg ttgctgtcgg ccggccgcga ggccaaggag cgtatcgtcg
     1381 agcgcgagtt cgcgcgcgtc ggcgacacgg tacgcaagga cctcgccggc taccccgaga
     1441 tgcaccggcg tctgagcgag gcgatcctgc gcatcgagga ggatcaggcg aaggcggtgg
     1501 aagtaccgcc ggaagcgccg ggctgggccc aggcggtgaa ggtcgtggcc gatctcgaca
     1561 cccgcaatgc cggtgccgac atcctcgccg acatccacaa gtcgatggtc aaggcgcacg
     1621 acgaggcgat gaacgactac cgcaaggcga gcggcgagcg ccacgccctg ctgcgccgca
     1681 tgatgcccga ctggcggctg atccaggaca cgctcgggcg cgtcaacaag agcgtcgaga
     1741 gtgtgatcgg ccgcgcgctc gcgatcgatc gtcacatgga ggagtacgag gcgatcgtgc
     1801 gcggcgagga tcgcgccgtg tcgatcctgt cctcatcgtc gatcgtgtac ttcttcattt
     1861 cgctgctggt gctcgcggtc gcgaccgccg gcaccgccgt caatttcacc ctgattgcgc
     1921 gcccgatggc ggagatggtc ggcggcacga acttcatcgg cgcctaccgc accgccgaca
     1981 tcgccgcgct ggtcatcatc atggtcgaga tctccatggg actgttcctc atggagtcgc
     2041 tgcgcatcac ccggctgttc ccggtgatcg gcgcactgtc cgacaagatg cgcgtgcgca
     2101 tgatcggcgt gacgttcacg atcctgctgc tgatggcaag catcgaggcg gggctcgcct
     2161 acatgcgcga ggtgctgctg caggatgagc tcgcgaccag cgcgctgctg cgcggcgata
     2221 cctctagcgc cggctcgatc gtctactcgc acctgtggat caccacggcc gcacagatgg
     2281 gcatgggctt cgtgctgccc ttcgcgctgg tgttcgtggc cattccgctc gaaaccttcg
     2341 tgcattcgct gcgcaccgtg gtcggcctga tcggcatcgc ggcgc
//
LOCUS       DATDMP010000371         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_949014, whole genome shotgun sequence.
ACCESSION   DATDMP010000371 DATDMP010000000
VERSION     DATDMP010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949014"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VI195_06480"
     CDS             <1..1182
                     /locus_tag="VI195_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEY6824068.1"
                     /translation="CIVDEARVPLLPADLERILIRRKGSDGFDAFLDRGRAPYTARQP
                     PSGTAEILYTSGSTGRPKGVVLSHAGQLWALSVHVAAQPTTGERSLIVAPLYHMNALF
                     NLSVCLMNGIEVVLMPRFEAAAHLKAAAEYRCTHLSGIPTMFALMARQADLIRTLDLS
                     CVKAVTIGSAPLTEALVERVQAMFPQAAISNGYGTTEAGPSIFEEHPGGVPRPLLSLG
                     YPLREVEVRLVGGTADEGVLQVRAPAMFTEYLNLPAVTHEKLHDGWYDTGDVMRRDAA
                     GFYYFTGRADDMFVCGGENVFPGEVEKMLEKHPAVLQAAVVPVPDEIKGQIPIAFVVL
                     RDRSVPLEELKRFSLASGPAYSHPRAFVVVDELPVASTHKLDKRPLMARALEAARALK
                     R"
     gene            complement(1192..1620)
                     /locus_tag="VI195_06485"
     CDS             complement(1192..1620)
                     /locus_tag="VI195_06485"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY6824069.1"
                     /translation="MNETPAAISLSELQQHLQGSPFHQLFDLACVRVDHEAGELELRM
                     RYTPSVERSPGTRQYHGGPIASLIDIAGDYVLWAVLGYGVPTINLRIDYLRPASATDL
                     RAVARIRRVGRTVGVVDIDVIDDQGRVIAIGRGSYGTKAG"
     gene            1877..>2745
                     /locus_tag="VI195_06490"
     CDS             1877..>2745
                     /locus_tag="VI195_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6824070.1"
                     /translation="MGKIQSNLPSAVAFASIAVSSLTTPSHAQTATSESTSSAALEEV
                     IVTAQKRTESVQDVPVSITAISARDLEAMGAEQFFDYGTDIPNLSFGIGASDGSLAGR
                     GIALRGIQGSNTTGFYIDETPVLETLDPHIVDIARIEVLRGPQGTLYGAESMGGTVRI
                     ITEQPDVSGKGVTGQVHLGGSWTEHGGFNNIEEGVVNIPLVSDTLAVRMSAFHQFDDG
                     WFNKDVGPYTAPPTGTIERLGSMRYYGAQLAIRWQPMTELSITPRIMYQKTEQDGDPY
                     AYNYAENLTQREV"
BASE COUNT          492 a          878 c          901 g          474 t
ORIGIN      
        1 tgcatcgtcg atgaggcgcg agtaccgctt ctgcctgcgg atctcgagcg cattctgata
       61 cgccgcaagg ggtccgatgg cttcgatgcc ttcctcgacc gcggccgcgc gccttacacc
      121 gcccggcaac cgccctcggg cacggcggag attctctata cctcggggtc aacgggccgc
      181 cccaaaggag tcgtgctgtc gcacgccggt cagctctggg cactcagcgt gcacgtcgct
      241 gcgcagccca ccaccggcga gcgctcgctg atcgtggcgc cgctctatca catgaacgcg
      301 ctcttcaacc tctccgtctg cctcatgaac gggatcgagg tggtgctaat gccgcgcttc
      361 gaggccgccg ctcatctcaa ggcggctgcc gagtatcggt gcacgcacct gagtgggatt
      421 ccgaccatgt tcgcgctgat ggcgcgccag gcggacctca tcaggacact cgacctctcc
      481 tgcgtgaagg ccgttaccat cggatcggcc ccgctcaccg aggcgctcgt cgagcgcgtg
      541 caggcgatgt ttcctcaggc ggccatctcg aacggctacg gcaccaccga agccggacct
      601 tcgatcttcg aggagcaccc tggcggggtg ccgcggcccc tgctgtcgct cgggtatccc
      661 ctgcgggagg tcgaagtacg cctcgtcggc gggaccgcgg atgagggggt cctgcaggtg
      721 cgcgcgcccg cgatgttcac cgagtacctg aatctgcccg cggtcacaca cgagaagctg
      781 cacgatggct ggtacgacac cggggatgtc atgcggcgcg acgcggcggg cttttattac
      841 ttcaccggcc gggcggacga catgttcgtc tgcggtggcg agaacgtctt tcccggcgag
      901 gtcgagaaga tgctggagaa gcacccggcg gtgctccagg ccgccgtggt tccagtaccg
      961 gatgagatca agggccagat tcccattgcg ttcgtggtgc tcagggatcg gagtgttccg
     1021 ctcgaggagc tgaaacgctt ctcgctggca tcagggccgg cgtacagcca tccgcgcgcc
     1081 ttcgtcgtgg tggatgagct gccggtggcg agcacacaca agctcgacaa gcggccactc
     1141 atggcgcggg cgctcgaggc ggcgcgggcg ctcaagcgct gaggaacagc tctagcccgc
     1201 cttggtcccg tagctgccgc ggccgatcgc gatgacccgg ccctgatcgt cgatgacgtc
     1261 gatgtcgacc accccgaccg tgcgtcctac gcggcggatg cgcgctacgg cccgcaggtc
     1321 cgtggccgag gcgggacgca ggtaatcgat gcgcaggttg atggtgggca ccccgtaacc
     1381 gagcacggcc cacaggacgt agtcgcccgc gatatcgatg aggctcgcga tcgggccgcc
     1441 atggtactgg cgcgttccgg gcgagcgctc gacggagggc gtgtagcgca tccgcagctc
     1501 gagctctccg gcctcgtgat cgacccgcac gcaggcgagg tcgaagagct gatggaaggg
     1561 cgagccctgc agatgctgct gcagctcact cagactgatc gcagcgggag tctcgttcac
     1621 tagcgtggca tcccgttcca gacgaagaaa atgtaactcc tttggcaccc actggccacc
     1681 gcggcaatat gcggtgagcg ctagggtggt gcaacaatcc cgcgggcgct tctatgtctg
     1741 gaagcatagc tttcccgtcg atttgcatgc tgtacttgcg gcggaactgc cggttaatag
     1801 cgggagggca agctgccagc ggcatgcctg cgcgagggtc gcgctgaaga gaaacagaca
     1861 acggggagac atccggatgg ggaagattca gtctaacctt ccgagcgcgg tcgcattcgc
     1921 gagcattgca gtcagctcgc tgactacacc cagccatgcg cagaccgcca ccagtgagtc
     1981 cacctcgagc gctgcgctcg aggaagtcat cgtcacggcc cagaagcgca ccgaatcggt
     2041 tcaggacgtg ccggtgagca tcacggcgat ctcggcacgg gacctcgaag ccatgggtgc
     2101 cgagcagttc ttcgactacg gcacggacat cccgaatctg tcgttcggca tcggtgcgtc
     2161 ggacggtagt ctcgccggcc gcgggatcgc gttacgtggc attcagggat caaataccac
     2221 cggcttctac atcgatgaaa cgccggtgct cgagacgctc gacccgcaca tcgtcgacat
     2281 cgcgcgcatc gaggtgctgc ggggcccgca gggtacgctc tatggagctg aatcgatggg
     2341 cggtacggtc cgcatcatca ccgagcagcc ggacgtgagt ggtaaggggg tcacgggcca
     2401 ggtgcacctc ggcggctcat ggaccgagca cggcggcttt aacaatatcg aggaaggggt
     2461 cgtcaacatc cctctcgtca gcgacacgct cgccgtgcgt atgagcgcct tccatcagtt
     2521 cgatgatggc tggttcaaca aggatgtggg gccgtacacg gcgccaccca ccggtaccat
     2581 cgagcgcctc ggcagcatga ggtactacgg cgcgcagctt gcgatccgat ggcagccgat
     2641 gaccgagctc tcgatcaccc cgcgcatcat gtatcagaag accgagcagg acggggatcc
     2701 ctacgcctac aactacgcgg agaaccttac acagcgcgaa gtgtt
//
LOCUS       DATDMP010000372         3426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1394137, whole genome shotgun sequence.
ACCESSION   DATDMP010000372 DATDMP010000000
VERSION     DATDMP010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394137"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VI195_06495"
     CDS             <1..781
                     /locus_tag="VI195_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824071.1"
                     /translation="CIGAVRLQNLEPCHEDIRRLTRALAGRNVRGFLNAASPGLITAF
                     QPNAYYPSHEAYLADLVSAMRPEYAAIAASGVQLQLDCPDLAMARHTGYQDLTDEQFL
                     KIAAANVEALNAATADIPPEKLRMHVCWGNYEGPHDHDIPLERVLGIILRARPATILF
                     EAANARHEHEWTVWAQAKIPEDKVLAPGLIDTRSNCVEHPELIAQRLQRFIAIVGVER
                     VIASTDCGFGTFAGYGKIDPAVTWKKLRALRAGADLATARL"
     gene            813..1259
                     /locus_tag="VI195_06500"
     CDS             813..1259
                     /locus_tag="VI195_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6824072.1"
                     /translation="MRSSVILLTAMLTVASALAHSQECKLKPKEVVTKFMNEFYVEKR
                     VREAFETWVEPGYIQHNPMAQTGRDAAIAFLEPFFKSHPDIHYSIARIIADGNLVAVH
                     SHGVFNPGERGLAIVDILRVQGCKIAEHWDVVQPVPEKSANTNGMF"
     gene            complement(1273..2175)
                     /locus_tag="VI195_06505"
     CDS             complement(1273..2175)
                     /locus_tag="VI195_06505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6824073.1"
                     /translation="MDFKGKTALITGGVSGIGFGIARAFAAADIDLVLTYRNAQYREQ
                     AAEWFRQQRRPQPRFIALDVTDRERWRAVAEEVGAVHILVNNAGVSVFGPTDAASYAD
                     YDWIMGVNFGGVVNGLVTFIPRIKSQGEGGHIVNVASMAAYLPGPQAGIYTASKYAVR
                     GLTECLRFNLAPYGIGVSLMCPGLTRTNAWDSALRRPAGFAASGFAPPERAELARFGQ
                     VFEAGMDPLEVGQKTLSGMQENRPVIFTHPEFAEDFDEIHRACRAALPQEPVPEERLR
                     IERLRRAAMRAAAAGQHIELDDLT"
     gene            2247..2945
                     /locus_tag="VI195_06510"
     CDS             2247..2945
                     /locus_tag="VI195_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6824074.1"
                     /translation="MSDLEARLEALEHRVGELEDINAIRRLHWAYGYYCDFNRAEDIV
                     GLFAEDGVVIFLSGEYHGHAGVRRLYGTWFQNYFTQGVDGPVDGFLLDHFQMQDIITV
                     APDRRTAKGRFRGLLLGGCHETRAYKPEGLPLQFFEAGLYENDYVREREVWKIKRLDY
                     MMQWQAEYEKGWSRTVAHLQPATRTYPADPHGPDVLLPAAAVRRTWPHRQDVPMHFAH
                     PRLGVALASKAMNP"
BASE COUNT          629 a         1142 c         1077 g          578 t
ORIGIN      
        1 ctgcatcgga gcggtacgcc tccagaatct cgagccctgt cacgaggaca tccgcaggct
       61 cacccgcgcg cttgcgggcc gtaatgtccg cgggtttttg aatgcggcgt cgccgggcct
      121 catcaccgca tttcagccca acgcctacta tccgagccac gaggcgtatc tggcggatct
      181 ggtgagcgcc atgcgaccgg agtacgccgc cattgccgcg agtggcgtgc agctgcagct
      241 cgactgtccg gacctggcga tggcgcgaca caccggttac caggacctca ccgatgagca
      301 gttcctgaag atcgccgcgg cgaatgtcga ggcgctgaac gccgccaccg cggacatccc
      361 gcccgagaag ctgcgcatgc acgtgtgctg gggtaattac gagggtcccc acgaccacga
      421 catcccgctc gagcgcgtgc tcggcatcat cctgcgcgct cgcccggcga ccatcctgtt
      481 cgaggcggcc aacgcgcgcc acgagcacga gtggacggtg tgggcgcagg cgaagatccc
      541 cgaggacaag gtgctcgcgc cggggctgat cgacacacgc tcgaactgcg tcgagcatcc
      601 cgagctcatt gcgcaacgtc tgcagcgctt catcgccatc gtcggcgtcg agcgcgtgat
      661 cgccagcacc gactgcggct tcggtacgtt cgccggctac ggcaagatcg atcccgcagt
      721 cacctggaag aaactgcgcg cactgcgcgc cggcgccgac ctcgccaccg cccgactctg
      781 atcattcata acgcaaccaa cgggagactc gcatgcgctc gtccgtcatc ctgctcaccg
      841 cgatgctgac cgtggccagt gcgctggctc attcgcagga gtgcaagctg aagccgaagg
      901 aagtagtcac caagttcatg aacgagttct atgtggagaa gcgcgtgcgc gaggcgttcg
      961 agacctgggt ggagcccggc tatatccagc acaatcccat ggcgcagacc ggccgcgacg
     1021 cggcgatcgc cttcctcgag cctttcttca agtcgcaccc tgacatccat tactcgatcg
     1081 cgcgcatcat cgccgacggc aacctggtcg ccgtgcactc acacggcgtg ttcaacccgg
     1141 gggagcgggg actcgcgatc gtcgatatcc tccgcgtcca gggctgcaag atcgccgagc
     1201 actgggacgt ggtgcagccg gtgccggaga aatccgccaa taccaacggg atgttctgag
     1261 cggccggctc gctcaggtca gatcgtcgag ctcgatgtgt tggcccgcgg ccgctgcacg
     1321 catcgccgcg cggcgcagcc gctcgatgcg cagccgctcc tccggcaccg gctcctgtgg
     1381 cagggcggcg cggcacgcac ggtggatctc gtcgaagtct tcggcgaatt ccgggtgagt
     1441 gaagatcacc ggccggttct cttgcatgcc gctgagggtt ttctgcccga cttccagcgg
     1501 atccatgccg gcctcgaaca cctggccgaa gcgcgcgagc tcggcgcgct cgggcggcgc
     1561 aaaccccgag gccgcgaagc cggcaggccg ccgcagcgcg gaatcccacg cgttggtgcg
     1621 ggtgagaccg gggcacatca gcgatacgcc gatgccatag ggtgccagat tgaagcgcag
     1681 gcactcggtc aagccgcgca ccgcgtactt gctcgcggtg tagatgccgg cctgcggacc
     1741 cggcagatac gcggccatcg aggccacatt gacgatgtgc ccaccctcac cctgagactt
     1801 gatgcgcgga atgaaggtca cgagcccatt caccaccccg ccgaagttca cgcccatgat
     1861 ccagtcgtag tcggcatagc tcgcggcgtc ggtggggccg aacacgctga cgccggcatt
     1921 gttgacgagg atgtgcaccg cacccacctc ctcagccacc gcacgccagc gttcgcggtc
     1981 ggtgacatcg agcgcgatga agcgcggctg agggcggcgc tgctgcctga accactccgc
     2041 cgcctgctcg cggtactgcg cattgcggta ggtcaacacc agatcgatgt ccgcggccgc
     2101 gaaggcgcgc gcaataccga agccgatgcc ggacacgccg ccggtgatga gtgccgtctt
     2161 gcctttgaag tccatgccgc ttcctctctc gcctcgctag gtaacatttg ttagcttttc
     2221 gcgcaaacca acgccggaga tgccccatgt ccgacctcga agcacggctc gaagctctgg
     2281 aacaccgcgt gggcgagctc gaggacatca atgccatccg ccgcctgcac tgggcctatg
     2341 gctactactg cgacttcaat cgggcggagg atatcgtggg gttgttcgcc gaggacggcg
     2401 tggtcatttt cctttccggc gaataccacg ggcatgccgg cgtgcgccgc ctctacggca
     2461 cctggtttca gaactacttc acgcagggcg tggatggacc ggtcgacggt ttcctgctcg
     2521 atcacttcca gatgcaggac atcatcaccg tggcgcccga ccggcgcacc gccaaaggcc
     2581 gctttcgcgg actgctgctc ggtggttgtc acgagacacg cgcgtacaaa ccggaaggac
     2641 tgcctctgca gttcttcgaa gcggggctgt atgagaatga ctatgtccgc gagcgcgagg
     2701 tctggaagat caagcggctc gattacatga tgcagtggca ggcggagtac gagaaaggct
     2761 ggtcgaggac cgtcgcgcac ctgcagcccg cgacgcgcac ctatccggcc gacccgcacg
     2821 gaccggacgt gctgctcccg gccgccgcgg tgcgccgcac ctggccacac cggcaagatg
     2881 tccctatgca ttttgcgcat ccgcgcttgg gcgtagcgct ggcctccaaa gcgatgaacc
     2941 cctgagcgca agcttccgcc ggaccgcggt ttcaggcctg ccggtacccc atagacggtg
     3001 tgcgttccac gcgccaccta agcgtggtgt gcacgccacg accccgaaat cgtatgtctt
     3061 gcaaacgacc aagttgtttg ttctacttac aaaaaataaa agggcattcc agtagcgcgt
     3121 cgtcaatgat cggcaactag aggggggacg tgagatggag gctactcggc gcgggttttc
     3181 ccacttgcga tggtgtgcgc taagcggctt cgcagcgctt ggcacagtgg tcagcggact
     3241 cgtcctggcg gcggatgccg cacccgcgac agcctcggga acagggctcc aggaggttgt
     3301 ggtaaccgcg caataccgtg aggagaaact gcaggacacg cccatcgcca tcaccgcgat
     3361 cacctcgcag cagatcgagg agcagggcgc gcaaaaactc tcggacatcc tgaccaacgc
     3421 gcccag
//
LOCUS       DATDMP010000373         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1837722, whole genome shotgun sequence.
ACCESSION   DATDMP010000373 DATDMP010000000
VERSION     DATDMP010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837722"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VI195_06515"
     CDS             complement(<1..611)
                     /locus_tag="VI195_06515"
                     /inference="COORDINATES: protein motif:HMM:NF036581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEY6824075.1"
                     /translation="MHVLRKPYLLYLGDAVLKSDCKTAFGLRDWCPEDVLGEWSHPQG
                     RVTLGLPRLSPAAAAAQGAGSIVIGVAPTGGQLPPHWQDDLEAALEAGLDVVSGLHTR
                     LGSLARLRAAARRCGRELIDVRHPTREFPVASGRKRTGKRVLTVGTDCALGKKYTALA
                     LTRALRALGVAATFRATGQTGILIAGSGIAVDALVADFIAGAA"
     gene            720..1700
                     /locus_tag="VI195_06520"
     CDS             720..1700
                     /locus_tag="VI195_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY6824076.1"
                     /translation="MSAPLEPVRPVTLAEIERARTTIARTVLRTPMVRLDLGAGRPDI
                     RLKLENLQPTNSYKLRGAANAIALLPDSDRRKGVWTISAGNAGQGVAYAARQADVSCA
                     VVVIETAPAAKIERMKALGAKLFPVPYEVAWQTLEARAFPGIEGTFVHPFDDHNFIAG
                     HATMGLEILEDAPDAAAVVAAIGGGGLISGIASAIKARSPHVKLFGAEPETAAPAARS
                     FALGSPQVFPGWTASFVDGAGGKSLFPRMWERMRPIVDGSIVVSLEQTKRAMRLLAEK
                     SRVVAEGAGALALAAALSGDAGAGPIVAVVSGGNIDLQKFCELIATAPAG"
     gene            complement(1710..>2328)
                     /locus_tag="VI195_06525"
     CDS             complement(1710..>2328)
                     /locus_tag="VI195_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824077.1"
                     /translation="MEHSGVWAQRYDEIDDLERARRLPSPQLVGTPEHSTLDLNVLQA
                     AGVEIVGRLAAIRDGRALFSGGLRNQFALADLKMHRLLDTFDTWARGRELDVEVSAPE
                     RFESTRAPAAPRLSLDLRSGEIRSIVWATGFHPDYRWLEVPVLDRKGELRHDGGVVVE
                     APGMYAIGLPLLRRRKSTFIIGAEDDARDLAGHLAGYLARDARSA"
BASE COUNT          388 a          801 c          782 g          357 t
ORIGIN      
        1 tcggcggcac cggcgatgaa gtccgcgacc agcgcatcca ccgcaatgcc gctgccggcg
       61 atcaggatcc cggtctgacc ggttgcccgg aacgtcgccg ccaccccgag tgcccggagc
      121 gcacgggtca gggcgagcgc ggtgtatttt tttccgagcg cacagtcggt accgacggtg
      181 agtacgcgct tgccggtgcg cttgcgtcca ctcgccaccg gaaattctcg cgtggggtgg
      241 cgcacatcga tgagctctcg cccgcagcgc cgcgcggccg cgcgcaggcg cgcgagcgag
      301 ccgagccgtg tatgtaagcc gctcaccaca tcgagccccg cctcgagtgc ggcttccaga
      361 tcatcctgcc aatgcggcgg cagttgcccg ccggtcggcg ccaccccgat gacgatcgat
      421 ccagcgccct gggcggcggc cgccgcggga gacaaacgcg gcaaaccgag cgtcacgcgc
      481 ccctgcgggt gcgaccactc gccgagcaca tcctcgggac accagtcgcg cagcccgaaa
      541 gcagtcttgc agtcggactt gagcacggca tcaccgagat acagcaggta cggtttgcga
      601 agtacatgca tgcgtgcagg actcgaacgg gctcgcggaa ctgatgatgc cacaggggcg
      661 cggcacggcc tgcctccctt tgcggtaatc tgccagtttg cacttgcagg tacccgccca
      721 tgtcggcacc tctggaaccg gttcggcccg tcacgctcgc ggagatcgag cgtgcgcgca
      781 caacgatcgc gcgcaccgtg ctgcgcaccc ccatggtccg gctcgacctg ggagccggcc
      841 ggccggacat ccggctcaag ctcgagaatc tgcagccgac caactcttac aagctgcgcg
      901 gcgcggcaaa tgccatcgcc ctgctgccgg actccgaccg tcgcaaaggg gtgtggacca
      961 tcagcgccgg taacgccgga cagggcgttg cctatgccgc tcgacaggcg gacgtgtctt
     1021 gcgcggtcgt ggtcatcgaa accgcgcccg cggcaaagat cgagcgcatg aaggccctcg
     1081 gtgcgaagct gttccccgtg ccgtacgagg tcgcctggca gacgctcgag gcgcgtgcct
     1141 tccccggtat cgaagggacg ttcgtgcatc ccttcgacga tcacaacttc atcgccggcc
     1201 acgcgaccat ggggctggaa atcctcgaag atgctcccga cgccgccgcc gtcgttgcag
     1261 cgatcggagg tggtggcctg atcagcggga ttgcgagtgc catcaaggcg agaagtccgc
     1321 acgtcaagct cttcggtgcc gagccggaaa ccgctgctcc ggcggcgcgc tcgtttgccc
     1381 tcggatcgcc gcaggtattt ccaggctgga ccgcctcgtt cgtcgatggg gcgggtggca
     1441 agagcctgtt tccgcgcatg tgggagcgga tgcgtccgat cgtcgatggc tcgatcgtcg
     1501 tcagcctcga gcaaaccaag cgcgcgatgc gcctgctggc ggagaagtcg cgtgtcgtgg
     1561 cggaaggcgc cggcgccctg gcgctggccg cggcactcag cggcgatgcg ggtgcaggcc
     1621 cgatcgtggc agtggtgtcg ggcgggaaca tcgacttgca gaagttctgc gagctcatcg
     1681 ccacggcgcc cgccgggtaa tcgccgcagc tatgcgctgc gcgcatcccg cgccagatag
     1741 cctgcgaggt gcccggctag atcgcgcgcg tcatcctcgg cgccaatgat gaaggtcgac
     1801 ttgcggcgcc gtaacagcgg caagccgatc gcgtacattc cgggagcctc cacgaccacg
     1861 ccaccgtcgt ggcgcagttc acccttgcgg tcgaggaccg gcacctcgag ccagcgatag
     1921 tccggatgga atccggtcgc ccacacgatc gagcggatct cgccgctgcg caggtcgagc
     1981 gacagccgcg gcgctgcggg cgcacgcgtg gactcgaagc gctcgggcgc ggaaacttcg
     2041 acatccaact cgcgtccgcg cgcccaggtg tcgaaggtgt cgagcagccg gtgcatcttg
     2101 aggtcggcga gcgcgaactg attgcgcagt ccacccgaga acagcgcacg cccatcgcgt
     2161 atcgcggcaa ggcgacccac gatctcgaca cccgcagcct gcagcacgtt gaggtccagg
     2221 gtcgagtgct cgggcgtacc gacgagctgc ggcgagggca gccggcgcgc acgctcgagg
     2281 tcgtcgatct catcgtaacg ctgagcccac acgcccgagt gctccatc
//
LOCUS       DATDMP010000374         2798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1283094, whole genome shotgun sequence.
ACCESSION   DATDMP010000374 DATDMP010000000
VERSION     DATDMP010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283094"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="guaA"
                     /locus_tag="VI195_06530"
     CDS             complement(<1..596)
                     /gene="guaA"
                     /locus_tag="VI195_06530"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY6824078.1"
                     /translation="MASVPRAANGTPAAAGAHPDIHAQRILILDFGAQYTQLIARRVR
                     ELGVYCEIHPWDLSDAQVRAFRPRGVILSGGPESVTGASSPHAPAAVFELGVPVLGIC
                     YGMQAMAHQLGGRVAASSEREFGYAEVTVTGPSRLLDKLEDRRNAAGRCVLDVWMSHG
                     DRVDALPAGFIASASTDTIPFAAMSDERRRFYGVQFHP"
     gene            complement(604..2070)
                     /gene="guaB"
                     /locus_tag="VI195_06535"
     CDS             complement(604..2070)
                     /gene="guaB"
                     /locus_tag="VI195_06535"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY6824079.1"
                     /translation="MRILEEALTFDDVLLVPAYSEILPREVDLSTQVTRRIRLNVPIL
                     SAAMDTVTEARLAITIAQEGGLGIIHKSMTIEAQAREVGRVKKFESGVIRDPITVSPS
                     MTIREVLDLTRSRGISGVPVVVGRKAVGIVTHRDLRFEDKLEAPVSSVMTPQERLVTV
                     PEDASKEQVLALLHRHRIEKLLVVNGSHELVGMITVKDFQKANEFPRACKDEGGRLRV
                     GAAVGTTPDTLERVAALRESGVDLVVVDTAHGHSRGVIETVKRIKAKWGDSQVVAGNI
                     ATAEAARALIEAGADAVKVGIGPGSICTTRVVAGVGVPQISAVANVAAALKDAQVPVI
                     SDGGIRFSGDIAKAVAAGAHAVMIGGLFAGTEESPGEVELYQGASYKSYRGMGSLGAM
                     AERHGSADRYFQDAATELEKLVPEGVEGRVPYKGSVVTILHQLSGGLRAAMGYTGSRN
                     ITDMRTRPVFVRITSAGVRESHVHDVSITKEAPNYRVS"
     gene            2125..>2798
                     /gene="xseA"
                     /locus_tag="VI195_06540"
     CDS             2125..>2798
                     /gene="xseA"
                     /locus_tag="VI195_06540"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY6824080.1"
                     /translation="MSAGYEIGALPAPTREVYSVSRLNREVRVLLERGLGVLWVEGEL
                     SNFSQPPSGHWYFSLKDRDAQLRCAMYRVKNALVGFAPRTGMQVLVRGRISLYEPRGE
                     FQLIVEHLEEAGVGALRREFERLKTRLAAEGLFAHERKRALPRFPRRIGVITSPAGAA
                     LRDILKILARRYPPAAVLIYPSAVQGAAAVPTLVGALTTASARAECDVLILARGGGSL
                     EDLWAF"
BASE COUNT          417 a          953 c          952 g          476 t
ORIGIN      
        1 tccggatgga attgcacgcc gtaaaagcgg cgccgctcat cgctcatggc agcgaatggg
       61 atcgtgtcgg tgctggcact ggcgatgaag cccgccggta acgcatcgac gcggtcacca
      121 tggctcatcc agacatcgag cacgcagcgg ccggcagcgt tgcgccgatc ctccagctta
      181 tcgagcaggc gcgaggggcc tgtgaccgtc acctccgcat agccgaactc gcgctcgctg
      241 gaggcggcca cccggccgcc gagctggtgc gccatggcct gcatcccgta gcagatgccg
      301 agcaccggga caccgagctc gaacaccgcg gcgggagcgt gcggggagct cgcgccggtg
      361 accgactcag gtcctcccga gaggatcacc ccgcgcggcc ggaaggcgcg cacctgcgca
      421 tcgctgaggt cccagggatg gatctcgcag tagacgccga gctcgcgcac gcgtcgcgcg
      481 atcagctgtg tgtactgcgc gccgaagtcg aggatcagga tgcgctgcgc gtggatgtcg
      541 ggatgcgcgc cggcggcggc gggtgtgccg tttgcagctc gggggacgct cgccatgggc
      601 gcttcaggac acccggtagt tgggtgcttc cttggtgatg ctgacatcgt gcacgtggct
      661 ctcgcgcact ccggcgctgg taatgcgtac gaacaccggc cgggtgcgca tgtcggtgat
      721 gttgcggctg ccggtgtagc ccatggcggc gcgcagtccg ccggagagct ggtgcaggat
      781 ggtgacgacg ctccccttgt atggcacgcg gccttccacg ccctcgggca cgagcttctc
      841 gagctcggtc gcggcgtcct ggaagtaacg atccgcggaa ccgtgccgct ccgccatggc
      901 accgagcgag cccatgccgc gataggattt gtaggaggcg ccctggtaga gctcgacttc
      961 gcccggggac tcctccgtac ccgcgaacag tcccccgatc atgaccgcgt gcgcgcccgc
     1021 agcgaccgcc ttggcgatgt cccccgagaa gcggatgccg ccatcggaga tcaccggcac
     1081 ctgcgcatcc ttgagcgccg cggcaacatt cgcgaccgcc gatatctgcg gtacacctac
     1141 cccggccacc acgcgcgtcg tgcagatgga gcccggcccg atgccgacct tgaccgcatc
     1201 cgcgcccgcc tcgatcagcg cacgcgccgc ctcggcagtc gcgatgttgc cggcgactac
     1261 ctggctgtca ccccacttcg ccttgatgcg tttcaccgtc tcgatgaccc cgcgggagtg
     1321 cccgtgcgcg gtatcgacca ccacgaggtc gacgcccgac tcgcgcagcg cggcgacccg
     1381 ctcgagagtg tccggggtcg tgcccaccgc cgcaccgacg cgcagccggc cgccctcgtc
     1441 cttgcaggcg cgggggaatt cgttggcttt ctggaagtcc ttcacggtga tcatgccgac
     1501 tagctcgtgc gagccattga ccaccagcag cttctcgatg cggtgcctgt gcagcagtgc
     1561 caacacctgc tccttcgagg catcctcggg caccgtgacc agccgctcct gcggcgtcat
     1621 gaccgaggac acgggagcct cgagcttgtc ctcgaaacgc aggtcgcggt gcgtcacgat
     1681 gccgaccgcc ttgcggccga cgaccaccgg cacgcccgag atgccgcgcg agcgggtgag
     1741 gtcgaggacc tcgcggatgg tcatgctcgg ggagaccgtg atcggatcgc ggatcacccc
     1801 gctctcgaac ttctttaccc ggccgacctc gcgcgcttgc gcctcgatgg tcatgctctt
     1861 gtgaatgatg ccgaggccgc cttcctgcgc gatggtgatc gcgaggcgcg cctcggtcac
     1921 cgtgtccatg gcggccgaca gtatcggcac gttcagccgg atgcggcgcg tgacctgggt
     1981 ggagagatcg acctcgcgcg gcaggatttc cgagtaggcc ggaaccagca gcacgtcgtc
     2041 gaaagtgaga gcttcttcga ggatgcgcat gcgcaattgt aagcgctggc ctcacaccgg
     2101 taaaccacgt gctatggtgc tcttatgtcc gctggctacg aaatcggtgc gttacccgct
     2161 cccacgcgcg aggtctacag cgtctcgcgg ctcaaccgcg aggtgcgggt gctgctcgag
     2221 cgtggcctcg gtgtgctctg ggtggagggc gagctgtcga acttctcgca gccgccctcc
     2281 ggacactggt acttctcgct gaaggaccgc gatgcgcagc tgcgctgtgc catgtatcgc
     2341 gtgaagaacg ccctggtggg ctttgccccg cgaacgggta tgcaggtgct ggtgcgcggg
     2401 cgtatcagcc tgtacgagcc gcgtggggag ttccagctga tcgtcgagca cctggaggag
     2461 gctggggtcg gcgcgctgcg gcgggaattc gagcgtctca agacccgact cgccgccgag
     2521 ggattgttcg ctcacgagcg caagcgcgcg ctgccgcgtt ttccgcggcg catcggtgtc
     2581 atcacctcgc cagccggcgc ggcgctgcgt gacatcctca agattctcgc gcggcgctat
     2641 ccgcccgcgg cggtgttgat ctacccgagc gcggtacagg gtgccgcggc ggtcccgacc
     2701 ctcgtcggcg cgctcactac ggcctcggcg cgcgccgaat gcgacgtgct gatcctcgcg
     2761 cgcggcggcg gctcgctcga ggatctgtgg gcgttcaa
//
LOCUS       DATDMP010000375         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_838281, whole genome shotgun sequence.
ACCESSION   DATDMP010000375 DATDMP010000000
VERSION     DATDMP010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838281"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VI195_06545"
     CDS             <1..949
                     /locus_tag="VI195_06545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824081.1"
                     /translation="CGKTMVHWFSTRERGLWVSAWNTAHNIGGFLISGLAFIGVWLFR
                     DWHAKFYFNALIAAAVAIGVYFLLLDTPQSRGLPPVEQYKDDYPAGYSDAAERTLTFR
                     DIVFRYVLPNRYLWAIATANVFCYFVRYGVENWIPTYFETAKSYTFKESSIGWILYEV
                     AAIPGTVACGWISDKWFKTQRAPAIILFMALTIVAVVVYWLNAKGPLWIDYAALTAIG
                     FLIYGPIMAIGLQSLDLVPKKAAGTAAGFTGLFGYVFGSAIAGTGVGWIADRWGWGGV
                     FGTMVACCLLTMLCSALTLGHRAQSTRRLADAGATPGSA"
     gene            complement(930..>2540)
                     /locus_tag="VI195_06550"
     CDS             complement(930..>2540)
                     /locus_tag="VI195_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6824082.1"
                     /translation="AAAAAHVATLKKLPAAVVTATALFHDGELAQSEQMVRNFLLEHG
                     HHVEAMRLLARIGMEHDVLDDAQLLLEAVLDLEPDYRAARFEYAQVLCRRHLYRESQQ
                     QTEKLLDVDRTNRDYRTLHAMTCVGLGQHERAIELYRALLPEAMHPAELHLSIAHSMK
                     TLGRQAPAIAEYHAAAAAREGFGDAWWSLANLKTYRFADAELEQMRAAEAAATTGAID
                     RYHLCFALGKALEDREEYAASFEYYARGNALKRAASRYRPEVIEQNTRLQIEVCSPEL
                     FARQQASGAPAADPIFIVGLPRSGSTLIEQILASHSKVEGTHELADIPRLVVELQGRD
                     WQLEAPRYPAVLAQMAADDLRRLGEKYLRDTRSYRSGKAHFIDKMPNNFRHIGLIHLI
                     LPNAKIIDARREPMACCFGNLKQLFAHGQDFAYSIEGIARYYRTYLELMRHWDAVLPG
                     RVLRVHYEDVVSDLEGNVRRLLDHCGLEFEPACVEFYRTERSVRTASSEQVRQPIYRE
                     ALDQWRHYESWLAPLRDALGDALTRYRE"
BASE COUNT          407 a          798 c          856 g          479 t
ORIGIN      
        1 gtgcggcaag acgatggtgc actggttcag cacgcgcgag cgcggcttgt gggtctcagc
       61 atggaatacg gcgcacaaca tcggcggctt cctgatctcg ggcctcgcgt tcatcggcgt
      121 gtggttgttt cgtgactggc acgcgaagtt ttatttcaac gcgctgatcg ccgccgccgt
      181 ggcaatcggc gtctatttcc tgctgctcga cacgccgcaa tcgcgcggcc ttccgccggt
      241 cgagcagtac aaggatgatt atccggccgg ttacagcgac gccgcagaac ggacacttac
      301 cttccgcgac atcgtattcc gctacgtgct gcccaaccgc tatctgtggg cgatcgccac
      361 agccaacgtg ttctgctatt tcgttcgcta cggtgtcgag aactggatcc cgacgtactt
      421 cgagactgcc aaaagctaca ccttcaagga gtcgagcatc ggctggatcc tgtacgaggt
      481 ggcggcgatc cccggcacgg tggcgtgtgg gtggatctcc gataaatggt tcaagacaca
      541 gcgcgcaccg gccatcatcc tcttcatggc gctgacgatc gttgcggtgg tggtgtactg
      601 gctgaacgcc aagggtccgc tgtggatcga ctacgccgct ctgaccgcca tcggtttcct
      661 gatctatggg ccgatcatgg ccatcggtct gcaatcgctc gacctcgtgc caaaaaaggc
      721 ggctggcacc gcggctggtt tcaccggtct ctttggttac gtgttcggct cggcaatcgc
      781 ggggaccggc gttggctgga tcgcggaccg gtggggctgg ggaggagtgt ttggcaccat
      841 ggtggcgtgt tgcctgctga cgatgctctg cagcgccctc acgctcggtc accgcgccca
      901 gagcacgcga cgcctcgcag acgcgggcgc tactcccggt agcgcgtaag cgcgtcgccg
      961 agagcgtcgc gcagcggggc gagccaggac tcgtagtggc gccactggtc gagcgcctcg
     1021 cggtagatcg gctgacgcac ctgctcggag ctcgcggtgc gcacactgcg ctcggtgcgg
     1081 tagaactcta cgcaggccgg ctcgaactcg agcccgcaat ggtcgagcaa gcgtcgcaca
     1141 ttcccttcga gatcactcac cacgtcctcg tagtgcaccc gcagcacgcg cccgggcagt
     1201 accgcatccc agtgccgcat gagctcgagg taggtgcggt agtagcgcgc gataccctcg
     1261 atgctgtagg cgaaatcctg cccgtgggcg aacagctgct tgaggttgcc gaaacaacac
     1321 gccatcggct cgcgccgcgc atcgatgatc ttcgcgttgg gcaggatcag atggatgagc
     1381 ccgatgtgcc ggaagttgtt cggcatcttg tcgatgaagt gagccttgcc gctgcggtag
     1441 ctgcgcgtgt cgcgcaggta cttctcgccg aggcggcgca aatcatctgc ggccatctgc
     1501 gccaataccg cggggtaacg cggagcctcg agctgccagt cccgaccctg cagctccacc
     1561 accaggcggg ggatgtcggc gagctcgtgc gtgccctcga ctttgctgtg tgaggcgagg
     1621 atctgctcga tgagtgtcga gccggagcgc ggcagaccga cgatgaagat cggatcggcg
     1681 gccggcgcgc cgcttgcctg ctgacgtgcg aaaagctcag ggctgcacac ctcgatctgc
     1741 aggcgcgtgt tctgctcgat gacctcgggt cgatagcggc tcgcggcgcg cttgagggcg
     1801 ttaccgcgcg cgtaatattc gaaggaggcc gcgtattcct cgcggtcctc gagtgcctta
     1861 ccaagcgcga agcacaggtg gtagcggtcg atcgcgccgg tcgtcgcggc cgcttccgcg
     1921 gcgcgcatct gctcgagctc cgcgtctgcg aagcggtagg tcttgaggtt ggcgagactc
     1981 caccacgcat cgccgaagcc ctcgcgcgcc gcggcggcgg cgtgatactc ggcgatcgcc
     2041 ggcgcctgcc ggccgagggt cttcatggag tgcgcgatcg acaggtggag ctcggcgggg
     2101 tgcatcgcct ccggcaacag cgcccggtag agctcgatgg cacgctcatg ctggccgagg
     2161 cccacgcagg tcatggcgtg cagggtgcga taatcgcggt tggtgcggtc cacgtcgagc
     2221 agcttttcgg tttgctgctg tgactcgcga tacaagtgtc gccggcacag cacctgcgca
     2281 tactcgaagc gcgccgcgcg atagtccggc tcgagatcga gcaccgcctc gagcagcagc
     2341 tgcgcgtcat cgagcacgtc gtgctccatg ccgatgcgtg ccagcaggcg catggcctcg
     2401 acgtggtggc cgtgctcgag gaggaaattg cgcaccatct gctcggattg cgcgagctca
     2461 ccgtcgtgga agagtgcggt agcggtcacc accgccgcgg gcaacttctt gagcgttgcg
     2521 acgtgcgccg cggcggcggc
//
LOCUS       DATDMP010000376         2702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1949593, whole genome shotgun sequence.
ACCESSION   DATDMP010000376 DATDMP010000000
VERSION     DATDMP010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949593"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VI195_06555"
     CDS             <1..372
                     /locus_tag="VI195_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6824083.1"
                     /translation="GVSHESLIRVAQLMEENIEKPLSLDEIASATELSRRQIERLFKR
                     HLNCVPKRYYLQLRLRRARELLLQTSMPIIDITTACGFQSPPHFSRCYRAQFGCPPSA
                     ERQSRQGKSPAPGGQTVTSVG"
     gene            517..1239
                     /locus_tag="VI195_06560"
     CDS             517..1239
                     /locus_tag="VI195_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEY6824084.1"
                     /translation="MADADAEFNLRDLNEQELIEQVHDDLYNGLKAEVEEATHIFLER
                     GWNAEKTLNDALVEGMRIVGIDFRDGILFVPEVLLAANAMKGGMEILRPLLAETGVPP
                     IGKIVIGTVKGDIHDIGKNLVSMMLEGAGFEVINLGINNPVEKYIAALEEHKPDILGM
                     SALLTTTMPYMKVVIDKMKEMGIRNDYIVLVGGAPLNEEFGKAVGADAYCRDAAIAVE
                     TAKSLLAKRRAAAGGAPAASAA"
     gene            1282..1905
                     /locus_tag="VI195_06565"
     CDS             1282..1905
                     /locus_tag="VI195_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY6824085.1"
                     /translation="MNSSPRALIIACGALAREIAALRRANGWDALEVRCLPAELHNRP
                     ERIAPAVREVIAETRGQYTQTFVAYGDCGTRGELDRVLREEGIERLPGAHCYEFFATA
                     RVFAQLADGEPGTFYLTDFLLRHFERLVVRPLGLDRHPELTQEYFRHYRKLVYLSQVP
                     RPAAIEQARRIAERFGFQFEHRFTGYGELGTRLAALVESQDTLAWPA"
     gene            1893..2201
                     /locus_tag="VI195_06570"
     CDS             1893..2201
                     /locus_tag="VI195_06570"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY6824086.1"
                     /translation="MASLSIISWRDIPAQVVVRHGRETAKVQLSARFQEAVDRAAMRA
                     GRGSSDAYLADWKRSDPRPCGADLQAEAAAEAARIEATYTDEALEALIRSKGLAAAGG
                     "
     gene            2202..2660
                     /locus_tag="VI195_06575"
     CDS             2202..2660
                     /locus_tag="VI195_06575"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824087.1"
                     /translation="MYAVRQWSVRHARGLNTLYRVLEAVLVTLHPLFRWIGYQRLEHP
                     VAAVERSVKGLLFDCRMCGQCILSSTGMSCPMNCPKNLRNGPCGGVRANGHCEVRPEM
                     KCVWVEAVAGAARIPGGAQALQEVQVAVDRRLQGKSSWLRVVRERVGEAS"
BASE COUNT          482 a          859 c          903 g          458 t
ORIGIN      
        1 ggcgtgagcc acgagagcct gatccgggtg gcgcagctca tggaggaaaa catcgagaaa
       61 ccgctctcgc tcgacgagat tgcgagcgct accgagctgt cgcgccgcca aattgaacgg
      121 ttgttcaagc ggcatctgaa ctgcgtaccg aagcgctact acctgcagtt gcggctgcgc
      181 cgggcgcggg aactgctgtt gcagacctcc atgcccatca tcgacatcac gaccgcctgc
      241 ggcttccagt cgccaccgca tttctcgcgt tgctaccgcg cacagttcgg gtgcccaccc
      301 agtgccgaac gacagtcgcg ccaggggaag tcccccgctc ccggtgggca aaccgtgacc
      361 tcggtcgggt gagcgagagg tccgaattcc ggctgtcgca agcggacatg ccgctgtcgc
      421 taagcaatca ggccgggcgg tatcgtagac cttaacattt gcctcacagc ggccgcgcga
      481 gccgctaaac ccaggtcgcg catggagatt tcgacaatgg ccgacgccga cgccgaattc
      541 aatttacgcg acctcaacga gcaggagctc atcgagcagg ttcatgacga cctgtacaac
      601 ggtctgaagg ccgaggtcga ggaggcgacc cacattttcc tcgagcgtgg ctggaacgca
      661 gagaagacgc tgaacgatgc gctggtcgag ggtatgcgca tcgtcggcat cgacttccgc
      721 gacggcatct tgttcgtacc ggaggtgctg ctcgcggcga acgccatgaa gggcgggatg
      781 gagatcctgc gcccgctgct cgcggagact ggtgtcccgc cgatcggcaa gatcgtgatc
      841 ggcaccgtca agggcgacat tcacgacatt ggcaagaacc tcgtgtcgat gatgctcgag
      901 ggcgccggat tcgaagtcat caatctcggc atcaacaacc cggtggagaa atatatcgcc
      961 gcgctggagg aacacaaacc cgacatcctc ggcatgtcgg cgctgctcac cacgacgatg
     1021 ccctacatga aggtcgtgat cgacaagatg aaggagatgg gcatccgcaa cgactacatt
     1081 gtgttggtgg gcggtgcgcc gctgaacgag gagttcggca aggcggtcgg tgctgatgct
     1141 tactgtcgtg atgccgccat cgcggtggaa actgccaaga gcctgcttgc caagcggcgc
     1201 gccgccgccg gtggtgcccc ggcagcgagc gccgcctgac agcgaccgtg ccggctgcac
     1261 gcacccacca tggaccaacc tgtgaactcg agcccccgtg cgctgatcat cgcctgcggc
     1321 gcactcgcgc gcgagatcgc ggcgctgcgc cgcgcgaacg gctgggacgc gctcgaggtg
     1381 cgctgcctgc cggcggagct gcacaaccgt cccgagcgca tcgcgccggc ggtgcgtgaa
     1441 gtcatcgcag agactcgcgg gcagtacacg cagacgtttg tggcgtatgg agactgtggc
     1501 acgcgcggag agctcgaccg cgtgctgcgc gaggaaggca tcgagcgctt gcccggcgcg
     1561 cactgctacg agttctttgc gaccgcacgc gtgttcgcgc agctcgccga cggcgagccc
     1621 ggcaccttct atctcacgga ctttctgctg cggcacttcg agcggctggt cgtgcggccc
     1681 ctcgggctcg accgccatcc ggagttgacg caggaatact tccgccacta ccgcaaactg
     1741 gtctatctct cgcaggtgcc gcggccagct gccatcgagc aggcacggcg aatcgcagag
     1801 cgcttcggct tccagttcga gcatcgcttc accggatacg gagagctcgg cacgcgcctt
     1861 gccgccctgg tcgagagcca ggacaccctg gcatggccag cctgagcatc atttcctggc
     1921 gcgacattcc cgcgcaggtg gtcgtgcggc acggacgcga gactgccaag gtgcagctat
     1981 cggcacgttt tcaggaagcg gtggaccgcg ccgccatgcg cgcgggccgc ggcagctccg
     2041 atgcgtacct cgccgactgg aagcgcagcg atccgcgacc gtgtggcgcg gaccttcagg
     2101 ccgaggccgc agccgaggcg gcgcgcatcg aggccaccta taccgatgaa gcactcgagg
     2161 cactgattcg ttcgaaggga ctcgcggcgg cgggcgggta agtgtacgca gtcaggcagt
     2221 ggtcggtgcg tcatgcccgc ggactcaaca cgctctatcg agtgttggaa gcggtactcg
     2281 tcacgctgca tccgcttttt cgctggatcg gctaccagcg actcgagcat cccgtggcgg
     2341 ccgtcgagcg ttcggtgaag ggcctgctgt tcgactgccg catgtgcggg cagtgcattc
     2401 tgagctccac cggtatgtcg tgcccgatga actgcccgaa gaacctgcgc aacgggccgt
     2461 gcggtggcgt gcgcgctaac ggtcactgcg aagtgcgccc cgagatgaag tgcgtgtggg
     2521 ttgaagcggt cgcgggagcc gcccgcattc cgggcggcgc gcaggcattg caggaggttc
     2581 aggttgccgt cgatcgccgc ctgcagggca agtcgtcctg gctgcgcgtg gtgcgtgagc
     2641 gcgtaggaga ggcgtcgtga tgttcgagga cgagcccgtt cccggttacc cgctgcccat
     2701 ca
//
LOCUS       DATDMP010000377         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_949666, whole genome shotgun sequence.
ACCESSION   DATDMP010000377 DATDMP010000000
VERSION     DATDMP010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949666"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VI195_06580"
     CDS             <1..1175
                     /locus_tag="VI195_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6824088.1"
                     /translation="AQGAGARVIAAGSDVSDAPEPYLRAGADIALKGEGLPALLELVR
                     RLDLAPRASSSALVEGVAGTATLGGAGLSAINGATLLPEVQLSERAAWDLVDMDRYRS
                     AWLSAHGYFSLNLAASRGCSFRCAWCSKPIWGNHYLQRSPTVVGQEMSYLKRTFAPDH
                     IWFADDIFGFRVDWVREFAAVCAKEGGAVPFTIQTRADLLNERMVEALREAACVEAWL
                     GAESGSQRILDAMNKGTTVAEIVIARSRLKDAGIRVGFFIQLGYLGEELADILATRDL
                     LASAQPDDVGVSVSYPLPGTRFHELVKAQLGPKTHWQDSDDLAMMFQGTYKSEFYRAV
                     RDLLHDQVSLQAPSAGARHDGSREARRSLERRWQRLLSHERQYRSPLRVQRASVAG"
     gene            1189..>2001
                     /locus_tag="VI195_06585"
     CDS             1189..>2001
                     /locus_tag="VI195_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6824089.1"
                     /translation="MKVSIIIAIYNEAGTAGALLERVWAQPLPGLTRELIIVESNSTD
                     GSRAIVADFVARRADSVHGEVHVIYQDRPHGKGHAVRAGFAAASGDILLIQDADLEYE
                     VSDYPDLLRPIVEGHTALVLGSRHLRPASWKIRQFANHGLLAGFMNAGGVVFRVLFNL
                     LFSVRLTDPTTMYKVFRASCLEGISFTCNGFDFDFELLGKLIRAGYVPLEVPISYKSR
                     GFDEGKKIRILRDPVAWLLAILRCRFSSTGRARRRLHEAAASRRSPSGVTVEA"
BASE COUNT          322 a          624 c          683 g          372 t
ORIGIN      
        1 cggctcaggg tgcgggggcg cgggtcattg cggccggctc ggacgtcagc gatgcgcccg
       61 agccctatct gcgtgcgggc gcagatattg ccctgaaagg cgagggtctg cccgccctgc
      121 tcgagctcgt gcgccgtctc gatctcgctc cacgggcctc gagctccgcg ctggtggagg
      181 gggtggcggg gaccgcaacc ctgggcggtg ccgggctgtc ggcaatcaac ggcgcgacgc
      241 tgctgcccga ggtgcagctg agcgagcgtg ctgcctggga cttggtcgac atggaccgct
      301 atcgcagcgc ctggctgtcg gcgcacggct acttcagcct caatctggcg gcctcgcgcg
      361 gctgctcgtt ccgctgcgcg tggtgctcca agcccatctg gggaaaccat tacctgcagc
      421 gcagccccac ggtggtggga caggaaatga gctacctcaa gcgcaccttc gcgcccgatc
      481 acatctggtt cgccgacgac atcttcgggt ttcgggtcga ctgggtgcgc gagttcgccg
      541 cggtctgtgc caaggagggt ggggcagtgc cgttcaccat ccagacgcgt gccgatctcc
      601 tgaatgagcg catggtggag gcgctgcgtg aggcggcatg tgtagaggca tggctcggtg
      661 ccgagagcgg cagccagcgc atcctcgacg cgatgaacaa gggcacgacg gtggcggaga
      721 ttgtcatcgc gcgctcccgc ctgaaggacg ccggaatccg cgtcgggttt ttcatacagc
      781 tcggctatct cggcgaggag ctggcagaca ttctggcaac gcgggatctg ctggcaagcg
      841 cgcagccgga cgacgtgggc gtcagcgttt cctatccact gcccggcacg cgcttccacg
      901 agctggtgaa ggcgcaactc ggacccaaaa ctcattggca ggacagcgac gatctcgcaa
      961 tgatgtttca gggaacctac aagtccgagt tctaccgcgc cgtgcgcgac ctgttgcacg
     1021 atcaagtgtc attgcaggcg ccttccgctg gggcacggca cgatggtagc cgtgaggcgc
     1081 gccggtcatt ggagcggcgc tggcagcgtt tgctgtccca cgagcgacag taccgctcgc
     1141 cgctgcgcgt gcagcgggcg tcggtggcag gctgaaatcc ggcgagccgt gaaagtctcg
     1201 atcatcatcg ccatttacaa cgaggcgggc accgcaggtg cactgctcga gcgtgtatgg
     1261 gcgcagccgc tccccggcct cacccgcgaa ctcataatcg tcgagagtaa ttcgaccgac
     1321 ggctcacgcg cgatcgtggc ggacttcgtt gcgcgccgtg cggattctgt gcacggcgag
     1381 gttcacgtca tctaccagga ccggccgcac ggcaaggggc acgcagtgcg cgcgggtttt
     1441 gcggcggcga gcggcgatat tctactcatt caggacgcgg acctcgaata cgaagtcagc
     1501 gattatccgg atctgctgcg gccgatcgtc gagggccaca ccgccttggt actcggcagc
     1561 cgtcacctgc gaccagcgag ctggaagatc cgacagttcg ccaaccatgg gctgctggca
     1621 ggcttcatga acgccggcgg cgtggtgttc cgggtgttgt tcaacctgct gttctcggtc
     1681 cgtctgacgg atccgacgac catgtacaag gtatttcgcg cgagttgcct cgagggaatc
     1741 agcttcacct gcaatgggtt tgatttcgat ttcgagctgc tgggtaaact gatccgcgcc
     1801 ggctatgtcc cgctggaggt tccaatctct tacaagtcgc gcggtttcga cgagggtaag
     1861 aagattcgta tcctgcgcga tccggtggcc tggttgctgg cgatcctgag gtgccgcttc
     1921 tcgagcaccg gccgcgcgcg ccgccgcctt cacgaggccg cggcgagtcg ccgctcgccg
     1981 agcggcgtca cggtcgaggc c
//
LOCUS       DATDMP010000378         2958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2061227, whole genome shotgun sequence.
ACCESSION   DATDMP010000378 DATDMP010000000
VERSION     DATDMP010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061227"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /gene="polA"
                     /locus_tag="VI195_06590"
     CDS             <1..1671
                     /gene="polA"
                     /locus_tag="VI195_06590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY6824090.1"
                     /translation="MGFAFGNEAYYLPLAHDYPGAPAQLGRDAALAKLRAWLESERAC
                     KVGHDLKFDSHVLANHGVRLGGCVHDTVLESYVLEVHERHDLGSLVQRHCGWQTVSYD
                     DLTGKGASRIGFSGVDLARATEYAAGEADCTLALHQILFPRVEAEPKLRFVYEKIEMP
                     VLPVLFRMERNGVLLDSAKLEAQSHELGREMLAIEQKAYEAAGQPFNLGSPKQIREIL
                     FDRQKLPVRKKTPTGEPSTDEDVLAELAQDHPLPKRILEYRAIAKLKSTYTDKLPKNV
                     NPRTGRVHTSYSQTTAVTGRLSSNEPNLQNIPIRTAEGRRIREAFVASPGWKIVSADY
                     SQVELRIMAHLSGDPNLRAAFARGDDVHRATAAEVFGVPLEQVSSDERRTAKVINFGL
                     IYGMSSFGVAQNLGIERATAQAYIESYFARYPGVKRYMDTTRELARTQGYVETVFGRQ
                     LWLPEIRSGSPARRQAAERAAINAPMQGTAADLIKLAMTQVQAFLDRERLETKLIMQV
                     HDELVLEVPDMETEHVKESVRALMQGVAQLEVPLIVDVGSGLNWDEAH"
     gene            complement(1668..2825)
                     /locus_tag="VI195_06595"
     CDS             complement(1668..2825)
                     /locus_tag="VI195_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824091.1"
                     /translation="MGLAGRSALDKVLAVRGGAELVALWAQVASVIELVTAVAAAGVG
                     TGLAVYVARTRRPERQRDFLREALRIGLGLGAPLAALVGAVSGLHADALSGGRLGAGM
                     FAFAALAAWLAIIPGLVNSYWLGQHLRERMLAFALGVAPLAVAAAWLAPDGFVVPAVI
                     VSQALPALALARVAISRAQAPRFRASSHPLRRYVLPGLSIGILGPAAMLLARGQVGEL
                     LSWHEAGVLQALWRLSDWVCAFAGGLLSLHYLPRFAAARGTGLLPAAMLHAAKELLIP
                     SAIAFAALWMLHRPLLHLLYGPGFEAGDGAAALFFAGSLVRVASWIPLFALYAMRRTL
                     AITAGEVLSLPLFAAAVMLTGGHLTLELAGWLWLGAYSAYAAFNAWAAARA"
BASE COUNT          578 a         1029 c          960 g          391 t
ORIGIN      
        1 atggggttcg ccttcggcaa cgaggcctat tacctcccgc tggcgcacga ctatccgggc
       61 gcgcctgccc agctcgggcg cgacgcggcg ctcgccaagc tgcgcgcctg gctggaaagc
      121 gagcgcgcat gcaaggtcgg ccacgacctg aagttcgact cgcacgtgct tgccaaccac
      181 ggcgtgcgcc tcggcggatg cgtccatgac acggtgctcg agtcgtacgt gctcgaagtg
      241 cacgagcggc acgatctcgg gtcgctggtg cagcgccact gcggctggca gaccgtgagc
      301 tatgacgact tgaccggcaa aggcgcttcg cgcatcgggt tttccggcgt tgacctcgcg
      361 cgggccaccg agtacgcggc gggcgaggcc gactgcacgc ttgcgctgca ccagattctc
      421 ttcccgcgcg tcgaggcgga gccgaagctc cgcttcgtct acgagaaaat cgagatgccg
      481 gtcttgccgg tgctgttccg catggagcgg aacggcgtgc tgctcgattc cgcgaagctc
      541 gaggcgcaaa gccacgagct cggccgcgag atgctcgcga tcgagcagaa ggcctacgag
      601 gcggccggcc agccgttcaa cctcggctcg cccaagcaga tccgggagat cctcttcgac
      661 cggcagaagc ttccggtgag gaagaagacg ccgaccggcg agccttccac cgacgaggac
      721 gtgctggccg agctcgcgca ggaccatccg ctgcccaagc gcatcctcga ataccgggcg
      781 atcgcgaagc tgaagtccac ctataccgac aagctgccga agaacgtgaa tccgcgcacc
      841 gggcgcgtgc atacctctta ttcgcaaacc accgcggtga ccggccggct gtcctcgaac
      901 gagccgaacc tgcagaacat ccccatccgc accgcggagg gccgccgcat ccgcgaggcc
      961 ttcgtcgcct cgcccggctg gaaaatcgtc tcggcggatt attcgcaggt cgagcttcgc
     1021 atcatggcgc acctctccgg cgatcccaac ctgcgcgctg cgttcgcacg cggcgacgac
     1081 gtgcatcgcg ccaccgcggc ggaagttttc ggcgtgccgc tcgaacaggt gagcagcgat
     1141 gagcggcgca ccgccaaggt catcaacttc ggcctgatct acggcatgtc gagcttcggc
     1201 gtcgcgcaaa acctcggcat cgagcgcgcc accgcgcagg cttatatcga atcgtatttc
     1261 gcgcggtatc ccggtgtgaa gcgctatatg gacaccacgc gcgagctcgc ccgcacgcag
     1321 ggttacgtgg agaccgtgtt cggccgccag ctatggctgc cggaaataag gagcggcagc
     1381 ccggcgcgcc gccaggcggc ggaacgcgca gccatcaatg cgcccatgca gggaaccgcg
     1441 gccgacctga tcaagctcgc gatgacgcag gtgcaggcgt tcctcgatcg agagcggctg
     1501 gaaacgaagc tcatcatgca ggtgcacgat gagctcgtcc tcgaagtccc cgacatggaa
     1561 acggagcacg tcaaggaaag cgtgcgcgcc ctcatgcagg gcgtcgccca gctcgaagtg
     1621 cccctcatcg tggacgttgg cagcggactg aactgggacg aggctcacta agcccgtgcc
     1681 gccgcccatg cattgaacgc cgcgtatgca ctgtaggctc ctagccatag ccagccggcg
     1741 agttcgagcg tcaagtgccc gccagtgagc atcaccgccg cagcgaacaa aggcagcgat
     1801 agaacttcgc cggcggtaat ggcaagcgtg cggcgcattg cgtaaagcgc gaacaacgga
     1861 atccacgacg ccacgcgtac caggctgccg gcgaaaaaga gcgcggcggc accatccccg
     1921 gcttcgaagc ccggcccgta gagaagatga agcagcgggc gatgcagcat ccacaaggca
     1981 gcaaaggcga tcgcggacgg tatcagcagc tctttggccg cgtgcagcat ggcggctggc
     2041 aaaaggccgg tgccgcgtgc cgccgcaaac cgcggcagat agtgaagcga cagcagccct
     2101 ccggcaaaag cgcataccca gtccgaaagc cgccacagcg cctgcagcac ccccgcttca
     2161 tgccaactga ggagctcacc cacttgtccc cgcgcgagga gcatcgccgc gggtcccaga
     2221 atgccgatgg agagtcccgg cagcacatag cgccgcagcg gatgcgagct cgcgcggaaa
     2281 cggggcgcct gcgcgcgcga aatcgcgacc cgcgccagag ctagcgctgg gagtgcttgg
     2341 ctcacgatga ccgccgggac gacgaagcca tccggagcaa gccaggcggc cgcgacggca
     2401 agcggcgcca cgcccagcgc aaacgcgagc atgcgttcac gcaggtgctg ccccagccaa
     2461 tagctattca cgaggccggg aatgatcgcc agccacgccg cgagtgcagc aaaagcaaac
     2521 atgccggccc caaggcgacc gcctgatagc gcatctgcat gcaggccgct cactgcaccc
     2581 acgagcgccg cgagcggcgc gcccaggccg agaccgatcc ggagagcctc gcgcaggaaa
     2641 tcgcgctggc gctccggccg gcgcgtgcgc gcaacgtaga ccgccaggcc cgtgccgacc
     2701 cccgccgcgg cgacagccgt gacgagctcg atcaccgagg cgacctgcgc ccacagcgcg
     2761 acaagctccg cgccgccgcg caccgcgagc accttgtcga gcgcggagcg acccgcgagg
     2821 cccactgcga tgatcgcgag cgtgaggccg agactcttaa ttcagcgaac cgaacacttt
     2881 gcgcaggatc gcgctgccgg cggccttacc cccgggccgc cgagaaaacc gtcggtgcgg
     2941 cccagcgacg ccaccgcg
//
LOCUS       DATDMP010000379         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1395152, whole genome shotgun sequence.
ACCESSION   DATDMP010000379 DATDMP010000000
VERSION     DATDMP010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395152"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VI195_06600"
     CDS             complement(<1..552)
                     /locus_tag="VI195_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY6824092.1"
                     /translation="MSSPDAPKPKKSVQLSGVLAGNTALCTVGRTGNDLHYRGYDILE
                     IAERCEFEEIACLLIHGRLPNRAELEAYKKQLASLRALPAAVRTALEALPAAAHPMDV
                     LRTGVSALGCILPEKDDHAAAGARDIADRLIASLGSMLTYWYHFSHSGERIHTETADD
                     STGAHFLHLLHRRPPSAPWVRAMH"
     gene            complement(557..1438)
                     /gene="prpB"
                     /locus_tag="VI195_06605"
     CDS             complement(557..1438)
                     /gene="prpB"
                     /locus_tag="VI195_06605"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY6824093.1"
                     /translation="MSERAVTSGARLRAAVEAEHPLQVVGAFNAYSALLARDAGFRAL
                     YLSGAGVANASFGLPDLGITSLNDVCEDVRRITAACELPLLVDADTGFGQAFNIARTT
                     RELIRAGAAGMHLEDQVSAKRCGHRPGKALVPPAEMVDRIKAALDARSDPDFVVMART
                     DAHAVEGQAAALERAAAYVAAGADMIFAEALGTLQEYREFAGRTGVPVLANLTEFGQT
                     PLFTLTELQAAGVRLVLYPLSAFRAAARAAATVYGAIRREGTQKSVVELMQTRAELYR
                     VLGYHEYERKLDELFGR"
     gene            complement(1448..>2215)
                     /locus_tag="VI195_06610"
     CDS             complement(1448..>2215)
                     /locus_tag="VI195_06610"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HEY6824094.1"
                     /translation="SADPRYRAGDAVLVTGCGLSETHDGGYSELARVPGDWVIPLPPG
                     LDPLSAMSLGTAGFTAALAIHRMERNGQTPARGPVVVTGASGGVGSIAIDMLAARGYP
                     VIAVSGKAAAVDYLQQLGAAQVLMRGELELGSKPLETARFAGAIDNVGGEILAWLTRS
                     VDFWGNIASIGLTGGAELKTTVMPFILRGVSLIGINSAATPRAWRLEVWQRIASDLRP
                     RHLARIVTRTISFAELPGAFPEYLAGRVTGRTVVRIA"
BASE COUNT          320 a          747 c          837 g          311 t
ORIGIN      
        1 gtgcatcgcc cgtacccagg gagcggaggg cgggcggcga tgcagcaggt gcaggaagtg
       61 cgccccggtc gaatcgtcgg cggtctcggt gtggatgcgc tcgcccgagt ggctgaagtg
      121 ataccagtag gtgagcatcg agccgaggga tgcgatcagc cggtcggcga tgtcgcgcgc
      181 gccggcggcg gcgtgatcgt ccttctccgg caggatgcac cccagggccg acaccccggt
      241 gcgcagcacg tccatcgggt gggcggcggc cggcagcgcc tcgagcgcgg tgcgcaccgc
      301 ggccggcagc gcgcgcagcg aggcgagctg cttcttgtag gcctcgagct cggcgcggtt
      361 gggcagccgg ccgtggatca gcaggcaggc gatctcctcg aactcgcagc gctcggcgat
      421 ttcgaggatg tcgtagccgc ggtagtgcag gtcgttgccg gtgcggccga cggtgcacag
      481 cgccgtgttg ccggcgagca ctccggagag ctgcaccgat ttcttcggct tcggggcgtc
      541 cggcgagctc atggactcac cttccgaaca gctcatcgag cttgcgctcg tactcgtggt
      601 aaccgaggac ccgatagagc tcggcgcgcg tctgcatgag ctccaccacg ctcttttgcg
      661 tgccctcgcg ccggatggcg ccgtacacgg tggctgccgc ccgggcggcg gcgcggaacg
      721 ccgacagcgg gtagagcacc agccgcaccc ctgcggcctg cagctcggtg agcgtgaaca
      781 gcggcgtctg gccgaactcg gtgaggttgg ctagcaccgg cacaccggta cgtcccgcga
      841 actcgcggta ctcctgcagc gtcccgagcg cctcggcgaa aatcatgtcg gcaccggcgg
      901 ccacgtaggc cgcggcgcgc tcgagcgccg ccgcctggcc ctcgaccgcg tgggcgtcgg
      961 tgcgcgccat gaccacgaaa tccgggtcgc tgcgcgcgtc gagcgccgcc ttgatgcgat
     1021 cgaccatctc ggcggggggc accagcgcct tgcccggacg gtggccgcag cgcttggcgg
     1081 acacctggtc ctcgaggtgc atgccggccg cccccgcgcg gatcagctcg cgcgtggtgc
     1141 gggcgatgtt gaaggcctgg ccgaatccgg tgtcggcatc gaccagcagc ggcagctcgc
     1201 acgccgcggt gatgcgccgc acgtcctcgc acacgtcgtt gagggaggtg atccccaggt
     1261 ccggcaggcc gaaggaggca ttggcaactc cggctccgga gaggtagagg gcgcgaaaac
     1321 cggcatcgcg cgcgagcagc gcggagtatg cgttgaacgc gcccacgacc tgcaggggat
     1381 gctccgcctc cacggcggcc cgcaggcgcg cgccggacgt gactgctcgt tccgacatgg
     1441 gcacagttta agcgatacgc accacggtgc ggccggtgac ccggcccgcg aggtactcgg
     1501 ggaaggcgcc ggggagctcg gcgaaggaga tggtgcgggt cacgatgcgg gcgaggtggc
     1561 ggggccgcag gtcggaggcg atgcgttgcc acacctcgag gcgccacgcg cgcggggttg
     1621 ccgcggagtt gatgccgatg agactcacgc cgcgcaggat gaacggcatt acggtggtct
     1681 tgagctcggc gccgccggtg aggccgatgc tggcgatgtt gccccagaaa tcgacgctgc
     1741 gcgtcagcca cgcgaggatc tccccgccca cgttgtcgat cgcgccggca aagcgcgcgg
     1801 tctcgagggg cttcgagccg agctcgagtt cgccgcgcat cagtacctgc gccgcgccga
     1861 gctgctggag gtaatccacg gcggccgcct tgccactcac cgcgatcacc gggtagccgc
     1921 gtgcggcgag catgtcgatg gcgatgctgc cgaccccgcc cgaggccccg gtgaccacca
     1981 ccggtccccg cgcgggcgtc tgcccgttgc gctccatgcg atggatggcg agcgcggcgg
     2041 tgaagcccgc ggtgccgagc gacatggcgc tgaggggatc gaggccgggg ggcagcggaa
     2101 tcacccagtc gccgggaacg cgggcgagct cggagtagcc gccgtcgtgc gtttccgaga
     2161 gcccgcagcc ggtcaccagc accgcatcgc ccgcgcggta gcgcgggtcg gcgga
//
LOCUS       DATDMP010000380         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_728663, whole genome shotgun sequence.
ACCESSION   DATDMP010000380 DATDMP010000000
VERSION     DATDMP010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728663"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VI195_06615"
     CDS             <1..603
                     /locus_tag="VI195_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY6824095.1"
                     /translation="AYGEYGGSSARAGGWGELFSDEGSAHWLAREGLRLFSRMSDGRS
                     PRGALHDILRRHFSLEQDLDLCAAIYGKSLAQRSQLAQLSRLVAAAATAGDPAARALF
                     AQAVTELADIVDAVRDQLQVPGELDLPLSYSGGLFQLRELLLEPFEAALAARARRYRL
                     SAARLPPDAGAALQAARLAGAPLAPSSIATLETQLTPGGD"
     gene            complement(637..2502)
                     /locus_tag="VI195_06620"
     CDS             complement(637..2502)
                     /locus_tag="VI195_06620"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY6824096.1"
                     /translation="MVAHSEKRPWLGLAVSVCAAALGASGLAACGDSDSSDTASPIKH
                     VIIVVGENRSFDHLFATYVPQHPEEGIHNLLSEQIVTASGAPGLLFSRAYQYRITSAP
                     NGGNYFMSADLKDKALYATLPPPDVNSAPPVSPNIALLNLPGGDPELLPQDQFLFGTG
                     GTGLSYTLGPDTRIADVFTLPPGPFQLTGPTMPFDAFTGDMSHLYFTMVQQADCAIDA
                     EHVSGDNPTGCLHDLQTAVNTTYATPAGTVPHDTGQTMAFFNMQQGDAPVSKYLADMY
                     TISDNYHQPVFGATWADSQALGFADQLYYSDGHGHPVTPDPSGNFNPDPQAGTLNLYR
                     ALGAWYDCSDEAQPGIGAIASYLRALPYAVSTNCSTGLYFSTFNVSPPFTPKGVRVAE
                     VPFVLPPTTQPSIGDVLSAHRISWKYYGGGFNEVGTNSLLATFCQICNPFQYEANYPD
                     IVPEHMRDVTDLFTDLANGTLPAVSYVKPDGYLDGHPGGGKWTLFEAFVKNLIQRAQS
                     NPRQWAGTAIFITVDESGGYYDSGFIQWVDFFGTGPRIPLIAVSPYSTGGHVSHVYNE
                     HSSFVKFIERNWKIQGTLSERSRDNLPNPIQDADNPYVPKNMPAIGDLFDMFQFP"
     gene            2568..>2945
                     /locus_tag="VI195_06625"
     CDS             2568..>2945
                     /locus_tag="VI195_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824097.1"
                     /translation="MSNRRAGYRGLVLALAILGTLSAVAQAPGGAGTHPAEHQHLDSR
                     FAHNRSYYDRGYAVHSPPDGGIANLHGPDGQRYYYHEGNWYHWRGTWGQSWRWDRWWH
                     GGWVVSAPPAGVFVPLLPPHYTVV"
BASE COUNT          527 a          884 c          981 g          553 t
ORIGIN      
        1 gcttatgggg agtatggcgg gagcagcgcg cgtgcgggcg gctggggcga gctgttcagc
       61 gacgagggct cggcgcactg gctcgcgcgc gaggggttgc gtctcttctc gcgcatgagc
      121 gacggccgct caccgcgcgg ggcactgcac gacattttgc gtcgtcactt ctctctcgag
      181 caagacctcg acctgtgcgc cgccatctac gggaaaagcc tggcgcagcg cagtcagctg
      241 gctcagctgt cgagactcgt cgccgcagcg gcaactgccg gagatcccgc ggcacgagca
      301 ctcttcgccc aggcggtcac ggagctcgcg gacatcgtcg atgcggtgcg cgatcagctg
      361 caggtgcctg gcgagctcga tctgccgttg tcgtattcgg gcggtctctt tcagttgcgt
      421 gagttgttac tggaaccctt cgaggccgca cttgcggctc gcgcacgtcg ttaccgcctc
      481 tcggccgcca ggctgccgcc agacgccggg gcagcgttgc aggcggcacg gctcgccggc
      541 gcgccactcg cgccgagcag catcgcaact ctcgagacgc aactcacccc cgggggagac
      601 tgaacacgca gtgccggaag ccggcgcacc gccctatcaa gggaactgga acatgtcgaa
      661 caagtcgccg atggccggca tattcttcgg cacatagggg ttgtccgcat cctggatggg
      721 gttcggcagg ttgtcgcgac tgcgctcgct caacgtgccc tgaattttcc agttgcgctc
      781 gatgaacttc acgaacgagg aatgctcgtt gtacacgtga ctcacgtgcc cgcccgtgga
      841 ataaggcgat accgcgatga gcggaatgcg cggtccggtg ccgaagaagt cgacccattg
      901 gatgaaccct gaatcgtagt agccgccgct ttcatccacc gtaatgaaga tggcggtccc
      961 ggcccactgg cgcggattgc tctgcgcgcg ctggatgaga ttcttcacga acgcctcgaa
     1021 cagcgtccat ttacctccgc cgggatggcc atcgaggtaa ccgtccggct tcacgtacga
     1081 cacggccggc aaggtgccgt tggcaaggtc cgtgaacagg tccgtcacgt cgcgcatgtg
     1141 ctcggggacg atgtcggggt aattcgcttc gtattggaag ggattgcaga tctgacaaaa
     1201 cgtggccagc agactgttgg ttccgacctc attgaagcca ccgccgtagt acttccagct
     1261 gatgcgatga gcgctcagca catcgccgat gctgggctgc gtagttggcg gaagaacgaa
     1321 cggtacctcg gccacgcgca ctcctttcgg ggtaaacggg ggcgagacgt tgaacgtcga
     1381 aaagtacagg ccggtgctgc aattggtgga gacggcgtag ggcaacgccc gcagataact
     1441 cgcgatcgcg ccgatccccg gctgtgcctc atccgagcag tcgtaccagg cgccgagcgc
     1501 acgatagaga ttgagggtgc cggcctgggg atcggggttg aagttcccgc ttgggtcggg
     1561 agtcaccgga tggccatggc catcgctgta atagagttga tcggcgaagc cgagcgcctg
     1621 gctgtccgcc caggtcgcgc cgaacaccgg ctggtgatag ttgtcactga tcgtgtacat
     1681 gtcggccagg tacttggaga ccggggcatc cccttgctgc atgttgaaaa atgccatcgt
     1741 ttgcccggtg tcgtggggga cggtgccggc aggcgtggca taggtggtgt tgaccgccgt
     1801 ctgcagatcg tggagacaac cggtcgggtt gtcgcccgaa acgtgctcgg catcgatggc
     1861 gcagtccgcc tgctgcacca tggtgaaata gagatggctc atatcgcccg tgaacgcatc
     1921 gaagggcatc gtgggcccgg tgagctgaaa ggggcccgga ggcaacgtga acacatcggc
     1981 gatgcgcgtg tcggggccaa gggtgtagct caaaccggtt ccgccggtgc cgaacaggaa
     2041 ttgatcctgc ggcagcagtt ctggatcgcc accagggaga ttgagcagcg cgatattagg
     2101 agagaccggt ggagcactgt tcacatcggg cgggggcagc gtggcgtaca gcgctttgtc
     2161 cttcaggtca gcgctcatga aatagtttcc gccattggga gctgaagtaa tcctgtattg
     2221 atacgccctg gaaaataaca atccgggtgc gccactggcg gtaacgatct gctccgacag
     2281 caggttgtgt atcccctctt cagggtgctg cggcacgtag gtcgcaaaca agtgatcgaa
     2341 gctgcggttc tcgcctacga cgatgatgac gtgcttgatc ggggacgcgg tgtcgctcga
     2401 gtcactatcg ccgcaagcgg ccagtccgct ggcgccgagt gccgccgcac aaaccgagac
     2461 ggcgaggcca agccaggggc gtttttcgct gtgtgcaacc acgacgtcgc ttcgatgggt
     2521 ccggacggcc tctcgtgtat attggcagtg tgcgtgatgc gcaagggatg tccaaccgga
     2581 gagccgggta ccgcggcctg gtgctcgccc tcgccattct gggaacgctg tcggccgtgg
     2641 ctcaggctcc gggcggtgcg ggcacccacc cggcggagca tcaacacctg gactcccgtt
     2701 tcgcacacaa tcgctcttac tacgaccgcg gctatgccgt gcactcaccg cccgatggcg
     2761 gcatcgccaa cctgcatggg ccggatggcc agcgctacta ctatcacgaa ggcaactggt
     2821 accactggcg gggcacctgg ggccaatcct ggcgctggga ccgctggtgg cacggcggct
     2881 gggtggtttc agctccaccg gccggggtat tcgtcccgct gctgccacct cactacacgg
     2941 tcgtc
//
LOCUS       DATDMP010000381         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1505270, whole genome shotgun sequence.
ACCESSION   DATDMP010000381 DATDMP010000000
VERSION     DATDMP010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505270"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..667)
                     /gene="secB"
                     /locus_tag="VI195_06630"
     CDS             complement(197..667)
                     /gene="secB"
                     /locus_tag="VI195_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY6824098.1"
                     /translation="MNETAGDMPLSNPADAGAVLALQNVYLKDCSYESPNGPRIDGNW
                     NPQINLDLQTSSTPLGPEVREVVLTVTVSAKHGEATIFLVEVKQAGLFVVRNLPESEL
                     KRAISTVCPGVLFPYARAAVSQLVSQGGFPQFLLPPVNFDALYAANQAQPAVTN"
     gene            complement(684..941)
                     /gene="grxC"
                     /locus_tag="VI195_06635"
     CDS             complement(684..941)
                     /gene="grxC"
                     /locus_tag="VI195_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY6824099.1"
                     /translation="MSAPKVVIYVTDWCPYCIRARSLLESRGIAIFEIDVEAVHGARE
                     EMRARSGRDTVPQIFIGERHVGGCDDLQALEADGRLDELLK"
     gene            complement(958..1380)
                     /locus_tag="VI195_06640"
     CDS             complement(958..1380)
                     /locus_tag="VI195_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6824100.1"
                     /translation="MERILDFASHHPWLATATAVLAALVVVYEMRLRSEGLRSVSPQE
                     LIRLMNQGALVLDLRPAEQYQAGHLNGARQMSSEQILSAADTLKKHKEKTVVVYDDSG
                     SLGAAAVRQLIAQGFTRVFALRGGLAAWRADNLPLSRA"
     gene            1379..>2170
                     /locus_tag="VI195_06645"
     CDS             1379..>2170
                     /locus_tag="VI195_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824101.1"
                     /translation="MGELAKPLTQKGAHYSVPVSCGTAFGLGGLSGGSAAPGARAARA
                     AMVMLILATLIAPPGTLSAAASAARPESDARKAEEQLQAVKAEIERVSREVSAEQVER
                     DRLTRELRSAELQVAQARDALSDVRHQRAEDAARRTALAAEKRAREKQLDENREALAG
                     QMRAAYLIGRQEPFKLLLNQKDPALAGRMFAYYSYFGRARAGQIHLIEDDVRRLAELE
                     KEQRAQLNDLEQARERRGHVLVSLEAQSRSRAQNLERLRAQQSGLE"
BASE COUNT          352 a          731 c          752 g          335 t
ORIGIN      
        1 aacgctcgtt gcagcgctgc tgcgccatcg ccgcaagccg cccgcgatca cgtccccaga
       61 gccgcaccgc gtgcccgccg cgggcgaact ggacggcgag cgcggtgccc caggagccgg
      121 ccccgagcac tgccactccg cccgcggacc gtgcgctcat cggcgctcca cgcgagccgc
      181 gcgccgtgcc cggtggtcag ttggtgacgg cgggctgcgc ctggttggcg gcgtacagcg
      241 cgtcgaaatt gaccggcggc agcagaaact gtgggaaccc gccctgactc acgagctgcg
      301 agaccgcagc gcgcgcgtac ggaaagagca ctccgggaca gacggtgctg atcgcacgct
      361 tgagctcgga ctcggggaga ttgcgcacga cgaaaagacc cgcctgcttc acctcgacca
      421 ggaagatggt cgcttcgccg tgcttggcgg agacggtgac ggtgagcacc acctcgcgca
      481 cctcgggtcc gagcggggtg gagctggtct gcagatcgag gttgatctgc gggttccagt
      541 tgccatcgat gcgcggtccg ttcggcgatt cgtacgagca gtccttcagg tagacgttct
      601 gcagcgcgag caccgcgccg gcatcggcag ggttggaaag cggcatgtcg ccggctgtct
      661 cattcatggt ctttccgctc cggttatttc aacagctcat ccaggcgtcc gtccgcctcg
      721 agcgcctgca ggtcatcgca tccgccgaca tggcgctcgc cgatgaagat ctgcggtacg
      781 gtatcgcggc cgctacgcgc gcgcatctcc tcgcgtgcac cgtgcaccgc ttcgacgtcg
      841 atctcgaaaa tggcgatccc tctggattcg agcagactcc gtgcgcgtat gcagtacggg
      901 caccagtccg tgacataaat gacgaccttc ggcgcactca tcgggctcgg tggcccctca
      961 ggcgcgcgag agcggcaggt tatcggcgcg ccaggccgca agccctccgc gcagcgcgaa
     1021 cacgcgcgtg aagccctggg cgatcagctg gcgcacggcc gcggcgccga gcgagccgct
     1081 gtcatcgtac acgaccaccg tcttttcctt atgcttcttc agcgtgtcgg cggcactcag
     1141 gatctgctcg ctgctcatct ggcgggcacc gttcaggtga ccggcctggt actgctcggc
     1201 ggggcgcagg tcgagcacga gcgccccctg gttcatgagg cggatgagct cctggggcga
     1261 aaccgagcgc aggccttccg agcgcaggcg catttcgtat acaacgacca gggcggccag
     1321 cacggcagtg gccgtggcga gccacgggtg atggctggcg aagtcgagga tacgttccat
     1381 gggtgagctg gctaagccgc tgacgcaaaa gggcgcgcat tatagcgtcc cggtgtcctg
     1441 cggcacggcc ttcggccttg gagggctttc gggcgggtct gcggcgccgg gcgcacgggc
     1501 cgcgcgggcc gcgatggtca tgctgatcct cgcgacgctc atcgcaccgc cgggcacgtt
     1561 gtccgctgcc gcgagcgcag cgcgtcccga gagcgatgcc cgcaaggccg aggagcaact
     1621 gcaggccgtc aaggcggaga tcgagcgagt gagccgcgaa gtgagcgccg agcaggtcga
     1681 gcgcgaccgg ctgacacgcg agctgcggtc cgcggaactc caggtcgcgc aagcgcgcga
     1741 tgcgctctcg gatgttcgcc atcagcgggc cgaggatgcg gccaggcgta cggcgctcgc
     1801 cgccgaaaag cgcgcccgtg agaagcagct ggatgagaac cgcgaggcgc tcgccggtca
     1861 gatgcgcgct gcctacctca tcgggcggca ggagcccttc aagctcctcc tcaaccagaa
     1921 ggaccccgcg ctcgccggcc gcatgttcgc ttactacagc tacttcggcc gtgcgcgtgc
     1981 cggccagatc catctcatcg aggatgacgt acggcgcctc gccgagctcg agaaggagca
     2041 gcgtgcgcag ctgaacgatc tcgagcaggc gcgcgagcgg cgtggccacg tgctcgtcag
     2101 tctcgaggct caatcgcgca gccgcgcgca gaatctcgag cgcctgcgcg cacagcagtc
     2161 ggggctcgag
//
LOCUS       DATDMP010000382         4589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1728980, whole genome shotgun sequence.
ACCESSION   DATDMP010000382 DATDMP010000000
VERSION     DATDMP010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728980"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VI195_06650"
     CDS             <1..509
                     /locus_tag="VI195_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY6824102.1"
                     /translation="MHGEHLGSAQLWAKLGCPYEQAIALSDDPGVELRKRALERFDAL
                     GARPMAMRLRRQLRAEGVRGLKRGANRSTRTNAAGLTVRELEVLTLVAQNLSNAAIAR
                     RLYLSAKTVDHHASAILAKLGIASRREAPDAARRLGIELGEHKRAAVLGVSRAVSFNQ
                     PGTEARNR"
     gene            629..1927
                     /locus_tag="VI195_06655"
     CDS             629..1927
                     /locus_tag="VI195_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824103.1"
                     /translation="MSGPAASATPDDDPRTRSPLRWLMLALAALGIFSSYYESDAIGP
                     ISDLLLRQRHLTQFQIGDLTAVISLPNILLAVFNGVLIDRYGPARVSLWAAVIGFSGA
                     VLTAIGTPYGLMWTGRLVFGVSEGAIFIALLAGVARWFPRSGVALATAIFLSLARVGS
                     YTLDKSATWARALYDAGWQPPLWLGAGITGIGLAAVLIFRILDGRWPPPPYARGARER
                     MNWGDVWGFDRSYWYILGLHCLYAAVFFPFRQTYAIEYLQHVKGLSLQQASDVNSGVF
                     AAAVLATPMFGLLADRIGHRALLLTVGTLLLPITLAVLSLTELNPWLSTVLMGVSFSM
                     VPAIIWPATTLIVEPRRLGTALGVITLLQNVALWGSNRIAGWLATQANAGPENPAGYD
                     TMIWFFGVVSLAALTSVLLLWQRESGPHGHGLENARAVAR"
     gene            1938..3038
                     /locus_tag="VI195_06660"
     CDS             1938..3038
                     /locus_tag="VI195_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY6824104.1"
                     /translation="MKISKLETFSNRYVCFVRLTGDSGAVGWGQTSTYNADITALVFH
                     RQVAPWALGANVGEIDALIERIEEREHKFPGSYRCRALAGLDTALWDLRGKLEGKPVT
                     QLLGGAPGPLRVYASSMRRDITPAQEAARFVRLRDAQGFDAFKWRVGAECGHDVDEWP
                     GRTEAVVPTVARALGDSVAKLVDANSGFSPGRAIEVGRMLEAEGISHYEEPCPYWRLE
                     ETKQVSDALGIDVTGGEQDCDLATWGRMIDMRAVDVVQPDIMYMGGIARTLKVARLAA
                     AAGLPCTPHSANLSLVTLCTMHLLGALPNAGKYLELSIEGPEYYPWQQGLFVEDPYTV
                     RDGRVTIPWAPGWGVEINPAWLEQATYQCSEG"
     gene            3208..>4589
                     /locus_tag="VI195_06665"
     CDS             3208..>4589
                     /locus_tag="VI195_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HEY6824105.1"
                     /translation="MHAAERVLQRLLPRHAAQFDLRSIEPRNGHELCRISPAGAHIRI
                     EGSTPSALLFGVNWYLKYLAHLQISTNGDQLDSAGDLPTPIDTIELQTPYAYRYALNE
                     NVDGYTTPYWDWPRWQREIDVLALSGINAMIVERGADLVLYRTFRDFGYSDAEIRAWI
                     TQPAHQNWQLMGNLCCFNGPISPELLQKRAASAQRIIARLRELGITPVMPGFYGIVPH
                     DFQRRFPAAHVIPQGEWAGFARPDWLDPRDRMFAQVASTFYRHQHELFGDSSIYDMEV
                     FQEGGDAGGVPVPEAARAVQRALWAAHPDASWMMLAWQGNPRQDLLAGVDRRHLLIID
                     IDHDAIPRDDRARDFQGAPFLFGGIWEFGGRTTLGANVGNITERLQRLGHSNANMAGT
                     ALFTEGVDTNPFAFDLFTEMAWRAAPLDISSWIASYVQRRYGVADPDAIQAWQVLAHT
                     AYDIRVDGVA"
BASE COUNT          778 a         1496 c         1507 g          808 t
ORIGIN      
        1 tcatgcatgg cgagcacctc gggtccgcgc agctgtgggc gaaactcggc tgcccatatg
       61 agcaggccat tgccttgagt gatgaccccg gtgtcgagct gcgcaagcgc gccttggaga
      121 gattcgacgc gctcggggca cggccgatgg ccatgcgact gcgccggcag ctcagggccg
      181 agggtgtgcg gggactaaag cgcggtgcca accgctcgac gcgcaccaat gcggcggggc
      241 tcacggtgcg cgagctcgaa gtgctgaccc tggtcgcgca gaatctctcg aacgccgcca
      301 ttgcgcgccg gttgtacctg tccgccaaaa cggtcgatca tcacgcgagc gcgatcctcg
      361 ccaaacttgg cattgcctcg cgccgcgagg cgcccgatgc agcacgcaga ctcggcatcg
      421 agctcggcga gcacaagcgc gccgctgtcc tcggtgtcag ccgcgcggtc tccttcaatc
      481 agccaggtac ggaagcgaga aatcgatgag cagaagggac cttgacccgc cgccgacggt
      541 gtaagtccgg atcggcagga aggaaaaact cgaactatcg cctgcgtgtc ggtcacaatc
      601 gcagcacaat gggcaacaca cacacgccgt gagcggtccg gccgcgtcgg ctacccccga
      661 cgacgatccg cgcacgcgct cgccgctgcg ctggctgatg ctggcgctcg cggcgctcgg
      721 catcttcagc agttactacg agtctgatgc gatcggaccg atctccgacc tgctgctgcg
      781 gcaacggcac ctgacccaat tccagatcgg cgatctcacc gcggtaataa gcctgccgaa
      841 catcctgctc gcggtcttca acggagtgct gatcgaccgc tatggcccgg cgcgcgtcag
      901 cctgtgggcg gcggtcattg gcttcagcgg cgcggtgctc acggcgatcg gcacgcccta
      961 cggtctcatg tggacggggc ggctcgtatt cggagtcagc gagggcgcga tcttcatcgc
     1021 gctgcttgcc ggggtagctc gctggttccc gcgcagcggc gtcgccctgg ccacggcgat
     1081 ctttctcagt cttgcacgcg tcggttccta cacgctcgac aagtcggcga cctgggcacg
     1141 cgcgctctac gacgccggct ggcagccgcc gctatggctc ggcgcgggta tcaccggcat
     1201 cggcctcgcc gccgtgctga tctttcgaat cctcgacggc cgctggcctc cacctccgta
     1261 tgcccgaggc gcgcgcgagc gaatgaactg gggggacgtc tggggcttcg atcgttccta
     1321 ctggtacatc ctcggcttgc actgcctgta cgcggcggtg ttctttccct tccgtcagac
     1381 ttatgccatc gagtacctgc agcacgtgaa gggtctgtcg ttacagcagg ccagcgatgt
     1441 gaactccggg gtcttcgcag cagcggtgct ggccacgccg atgttcggac tgctggccga
     1501 ccgcatcggg caccgcgcgc tgctgctcac cgtcggcacc ctgctgctgc cgatcacgct
     1561 tgccgtgctg agcctcaccg agctgaatcc gtggctctcg acggtgttga tgggagtgag
     1621 cttctcgatg gtcccggcca tcatctggcc ggccacgacg ttgatcgtcg agccgcgccg
     1681 gctcggcacc gcccttggag tcatcacctt gctgcagaat gtggcgctgt ggggctcgaa
     1741 ccgcatcgcc ggctggctcg cgacccaggc gaacgcaggg ccggaaaacc cggctggcta
     1801 cgatacgatg atctggttct tcggtgtggt gagcctcgcc gcactcacct ccgtcctgct
     1861 gttgtggcag cgcgagagtg gcccgcatgg acacggactg gagaatgccc gcgccgtcgc
     1921 gcgctaagaa tgccgccatg aagatcagca agctggagac cttcagcaac cgctacgtct
     1981 gtttcgtgcg cctcaccggt gacagcggcg cggtgggctg gggtcaaact tccacctaca
     2041 atgccgatat cactgcgttg gtctttcacc gccaggtcgc accctgggca ctcggcgcga
     2101 acgtcgggga aatcgatgcg ctgatcgagc gcatcgagga gcgcgagcac aaatttcccg
     2161 gcagctatcg ctgccgcgcg ctcgcaggac tcgacaccgc actgtgggat ctgcgcggca
     2221 agctcgaggg caagccggtc acacagctgc tgggcggcgc gccggggccg ctgcgtgtgt
     2281 acgcttcctc catgcgccgc gacatcaccc cggcgcagga ggccgcacgc ttcgtgcggc
     2341 tgcgagatgc gcagggcttc gatgcgttca agtggcgtgt gggcgcggag tgcggccacg
     2401 acgtcgatga gtggccaggt cgcaccgagg cggtcgtgcc cacggtggcg cgcgcactcg
     2461 gtgacagcgt cgccaaattg gtcgatgcca acagcggctt ctcgcccggt cgtgccatcg
     2521 aagtgggacg gatgctcgag gcggagggta tcagccacta cgaggagccc tgcccctatt
     2581 ggagactcga ggagaccaag caggtaagcg acgcgctcgg gatcgatgtc actggcggtg
     2641 agcaggactg cgatctggcc acgtgggggc gcatgatcga catgcgagcg gtcgatgtgg
     2701 tgcagccgga catcatgtac atggggggca tcgcgcgtac tctcaaagtc gcgcgcctgg
     2761 cggcggcggc cggactgccg tgcacgccgc acagcgccaa cctgtcgctg gttacgcttt
     2821 gcaccatgca cctgctcggc gcgctgccga acgccggcaa gtacctggag ctttcgattg
     2881 aaggaccgga gtactacccg tggcagcagg gactattcgt cgaagatccg tacacggtgc
     2941 gcgacgggcg agtgaccatt ccatgggctc caggttgggg ggtcgagatc aatcccgcct
     3001 ggctcgagca ggcaacctac cagtgcagtg aggggtagag cagaacgctc acgccgcgcg
     3061 gccgcaacgg tgcagggttt agaacggcga gagagctgcc cgatggcaaa ccgatcgcga
     3121 catcgcccgc gccgccgcgt gctgcccctg ggcgtcctgc tagggctcgg gttgctaccg
     3181 ggctcggcgt catcgacggg ctttgacgtg cacgccgccg agcgtgtgct gcagcggctg
     3241 ctgccgcggc atgcggcgca attcgatttg cgttcgatcg agcccagaaa cggtcacgag
     3301 ctgtgccgca tctcacccgc tggcgcacac atcaggatcg agggcagcac cccgagcgcg
     3361 ctgctgttcg gggtgaactg gtacctcaag tatctcgcgc acctgcagat ctcgaccaac
     3421 ggcgaccagc tcgatagcgc aggcgattta ccgacgccca ttgacaccat agagctgcag
     3481 acgccctacg cctaccgcta tgcgttgaat gagaacgtgg atggctacac cacgccgtac
     3541 tgggattggc cgcgctggca gcgtgagatc gacgtactgg cactttccgg catcaacgcc
     3601 atgatcgtcg agcgtggcgc cgacctggtc ctctatcgaa cgttccgcga cttcggctac
     3661 tcggatgccg agatacgtgc ctggatcact caaccggcac accagaactg gcaactgatg
     3721 ggcaacctgt gctgcttcaa cggcccgatc agcccggagc tgctgcaaaa gcgcgcggca
     3781 tcggcgcaga gaatcatcgc gcgcctgcgc gagctcggca tcacgccggt gatgccgggc
     3841 ttctacggca tcgtgccgca cgacttccag cgcagatttc ccgctgcaca tgtcattccg
     3901 cagggcgaat gggcgggctt cgcgcggccg gactggctgg atccgcgcga tcggatgttt
     3961 gcccaggtgg ccagtacgtt ctaccgacat caacacgagc tcttcggcga tagcagcatc
     4021 tacgacatgg aggtgttcca ggagggcgga gacgccggcg gcgtgcccgt gccggaagcc
     4081 gcccgtgccg tgcagcgggc tttgtgggca gcacatcccg atgccagctg gatgatgctc
     4141 gcctggcaag gcaatccacg ccaggatttg ctcgccggcg tcgaccggcg gcatttgctg
     4201 atcatcgaca tcgatcacga tgcgataccg cgcgatgacc gcgcacggga tttccagggt
     4261 gcgccgttcc tgtttggcgg catctgggag tttggcggcc gcactacgct cggcgcaaac
     4321 gtgggcaaca ttaccgagcg cctgcagcgc ctcggccaca gcaacgcaaa catggccgga
     4381 actgcgctgt tcaccgaagg cgtggatacc aatccattcg ccttcgacct tttcaccgag
     4441 atggcctggc gcgccgcgcc gctcgacatc tccagctgga tcgccagcta tgtgcagcgc
     4501 cgctatggcg tggccgatcc cgatgcgatt caggcatggc aggtgctggc gcacaccgct
     4561 tacgacatcc gcgtggatgg ggtagcttt
//
LOCUS       DATDMP010000383         3437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1839388, whole genome shotgun sequence.
ACCESSION   DATDMP010000383 DATDMP010000000
VERSION     DATDMP010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839388"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1291)
                     /gene="gspF"
                     /locus_tag="VI195_06670"
     CDS             complement(77..1291)
                     /gene="gspF"
                     /locus_tag="VI195_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY6824106.1"
                     /translation="MGAFEYTALDASGKERKGILEGDTPRHIRQLLREQQLLPVTVAE
                     VAQKEATRQRSFSLIRRVSAADLALFTRQLATLVRAGLPLEESLLAVSQQTEKPRVQS
                     IILGVRARVMEGHTLADGFAEFPRVFPEIYRATVAAGEQAGHLDNVLERLADYTEGRD
                     QLRQKVVAAMLYPIVLTVLCFAIVSALLVYVVPQVVSVFESSKAKLPLATQVLIATSG
                     FLRAYGIYLLIGVVLALLLFRRWLRNPDARRRFHRFQLRLPLVGKLARGFNTARFTRT
                     FSILSASAVPVLEALRIAGEVVTNLPMRDAVTEAAARVREGAPIGRSLAASRLFPPMT
                     IHLISSGESSGQLESMLERAAISQERELDGLLAALVGLLGPVLIVCMGLFVMGIVFAM
                     LLPIFEINQLIH"
     gene            complement(1431..2936)
                     /gene="gspE"
                     /locus_tag="VI195_06675"
     CDS             complement(1431..2936)
                     /gene="gspE"
                     /locus_tag="VI195_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY6824107.1"
                     /translation="MNDVAHVHAAGVDDAPGRRLSFAFAKRHGVLVHRFVDGVAECIY
                     RDTATPQALAEVRRYLRCPLRLARVPEAQFDELLRQAYEAGSDAMQAVEGLDDTTDLA
                     HLAQDLPEQADLLDSEDDAPIIRLINAVLTQAVKENASDIHIEPFENRLVIRFRVDGV
                     LREVLQSRRAVAPLVVSRVKVMSRLDIAEKRLPQDGRISLKIAGRAVDVRVSTIPSGH
                     GERVVLRILDKQAGRLDLNSLGMDPHTQGAMDELIHKPHGIILVTGPTGSGKTTTLYA
                     ALERLNDNTQNIMTVEDPIEYYIDGIGQTQVNTKVEMTFARGLRAILRQDPDVVLIGE
                     IRDLETAQIAVQASLTGHLVLSTLHTNTAAGAITRLRDMGIEPFLLSSSLIGVLAQRL
                     VRVLNPETKQPFQAGEYERRLLNLRSDEPSPTLYRPGRDAAGGYRGRSGIYELIIVDD
                     AMRTMIHDGVSEQEIERHARLSTPSIRDDGRARVLRGETTIEEVLRVTRED"
     gene            complement(2933..>3437)
                     /locus_tag="VI195_06680"
     CDS             complement(2933..>3437)
                     /locus_tag="VI195_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824108.1"
                     /translation="DDMSKGESRVPYLGSIPILGLLFKTRNQSSTKNNLMIFIRPQIM
                     RDAHQAAYETELKYNYMQDQQHPMFHTSPSDVPPLLPGVPPGKLPPLPPPPPPGKDSA
                     QGPAAPGAAGAAPPAAAKPSGSQPGEPTATPGTVPPGAPAPDNAATPAGSPPPQGDTA
                     PPPQSKP"
BASE COUNT          518 a         1099 c         1240 g          580 t
ORIGIN      
        1 cggcaggact gcaatgtcac gcgcctggtt tgtgaagtgg tccgctttcg tggctggtac
       61 ggccgagcgg ggcgtctcag tggatcaact ggttgatctc gaagatcggc agcagcatgg
      121 cgaacacgat ccccatgaca aacagtccca tgcacacgat cagcaccggg ccgagcagtc
      181 ccaccagcgc cgccagcaga ccgtcgagtt cccgttcctg gctgatggcg gcccgctcga
      241 gcatgctctc gagctgcccg ctcgattcgc cgctggagat caggtggatg gtcatgggcg
      301 gaaacaaccg gctcgcagcg agtgagcggc cgatgggcgc accctcgcgc acccgggcgg
      361 ccgcctcggt caccgcatcg cgcatcggca ggttggtgac cacttccccg gcaatgcgca
      421 gcgcctcgag caccggcacg gcgctcgccg aaaggatgct gaaggtgcgt gtgaagcgcg
      481 cggtgttgaa gccgcgcgcg agcttgccga ccagcggcag gcgcagctgg aagcggtgga
      541 agcggcggcg ggcgtcggga ttgcgcagcc agcgccggaa cagcagcagg gcgagcacga
      601 ccccgatgag caggtagatc ccgtaggcgc gcaggaagcc gctggtggcg atcagcacct
      661 gcgtcgccag cggcagcttc gccttgctcg attcgaacac cgacaccacc tgcggcacca
      721 cgtacacgag cagcgccgac acgatcgcga agcacagtac cgtgagcacg atcgggtaaa
      781 gcatcgccgc gaccaccttc tggcgcagct gatcgcgccc ttcggtgtag tccgcgagcc
      841 gctcgagcac gttgtcgagg tgtccggcct gctcgccggc ggcgaccgtg gcccggtaga
      901 tctcgggaaa gacgcggggg aattcggcga agccatcggc gagggtgtgg ccttccatca
      961 cccgggcgcg tacgccgagg atgatgctct gcacccgcgg tttctcggtc tgctgcgaca
     1021 ctgcgagcag cgactcctcg agcggcagcc ccgcgcgcac cagggtggcg agctggcggg
     1081 tgaacagcgc cagatccgcc gccgagacgc gcctgatcag gctgaaggag cgctggcgcg
     1141 tggcttcttt ctgtgcgacc tcggcgaccg tcaccggtag cagctgttgc tcgcgcagca
     1201 gctggcggat gtgccgcggc gtgtcgcctt cgaggatccc cttgcgctcc ttgccggagg
     1261 catcgagcgc ggtgtattcg aacgcgccca ttcttcttag attagctgaa agtcgcctgc
     1321 ggctctttca gcagacggcg gctgagcttc gttcagcgcc ggagcgaaag ccggggcctt
     1381 cgcccctccg tggagcgggg cacgagccct atccggtgca gtcaattccg tcagtcctcg
     1441 cgcgtaacgc gcagcacttc ctcgatggtg gtctccccgc gcagcacgcg cgcgcggccg
     1501 tcgtcgcgga tcgagggggt gctgagccgc gcgtggcgct cgatctcctg ctcggatacc
     1561 ccgtcgtgaa tcatggtgcg catggcgtca tcgacgatga tgagctcgta gatacccgag
     1621 cggccgcggt agccaccggc tgcgtcgcgc ccggggcggt agagcgtcgg cgagggctcg
     1681 tcgctgcgca ggttgagcag ccgccgctcg tactcgcccg cctggaaggg ttgcttggtc
     1741 tcgggattga gcacgcgcac gagccgctgg gccagtaccc cgatcaggct cgaggagagc
     1801 aggaagggct cgatgcccat gtcgcgcagg cgcgtgatgg cgccggcggc agtgttggta
     1861 tgcagcgtcg agagcaccag gtggccggtg agactcgcct gtacggcgat ctgcgcggtc
     1921 tccagatcgc ggatctcgcc gatcagcacc acatccgggt cctggcgcag gatggcgcgc
     1981 agcccgcgcg caaaggtcat ctcgaccttg gtgttgacct gggtctgacc gataccgtcg
     2041 atgtagtact cgatcggatc ctcgaccgtc atgatgttct gcgtgttgtc gttcaggcgc
     2101 tcgagcgcgg catagagcgt ggtggtcttg ccggagccgg tggggcctgt gaccagaatg
     2161 atgccgtgcg gcttgtggat gagctcatcc atggcaccct gggtgtgcgg atccatgccg
     2221 agggagttca ggtcgagcct gccggcctgc ttgtcgagaa tacgcagcac cacgcgctcg
     2281 ccgtgcccgg aggggatggt ggatacacgt acatcgaccg cacggccggc gatcttcagg
     2341 ctgatgcggc cgtcctgcgg caggcgtttc tcggcgatgt cgaggcgcga catgaccttc
     2401 acgcgcgaca ccacgagcgg cgcaaccgca cggcgcgact gcagcacctc acgcagcacg
     2461 ccatcgacgc ggaagcggat caccagacgg ttctcgaacg gctcgatgtg gatgtcggag
     2521 gcgttctcct tgaccgcctg agtgagcacg gcgttgatca ggcggatgat cggcgcatca
     2581 tcctcgctgt cgagcaggtc ggcctgctcg ggtagatcct gggcgaggtg cgcaaggtcg
     2641 gtcgtatcgt cgagtccctc caccgcctgc atcgcatccg agcccgcctc gtacgcctga
     2701 cgcagcagct catcgaactg cgcctccggc acgcgcgcga gccgcagggg gcagcgcagg
     2761 tagcggcgca cctcggcgag cgcctgcggt gtggcggtgt cgcggtagat gcactcggcg
     2821 acgccgtcga cgaagcggtg caccagtacg ccgtggcgct tggcgaatgc gaatgacagg
     2881 cgcctgccgg gggcgtcgtc gacgcccgcg gcgtggacat gggcgacatc gttcatggct
     2941 tgctctgggg cggcggggcg gtgtcgcctt gaggcggcgg gctgcccgcg ggggtcgcag
     3001 cgttgtcggg agccggtgca cccggtggca ccgtgccggg ggtagccgtc ggctccccgg
     3061 gctgtgagcc cgagggcttg gcggccgctg gcggtgccgc gccggcagcg ccgggcgcgg
     3121 ccggcccctg cgcgctgtcc ttgcccggcg gcggcggcgg cggcagcggc ggcagctttc
     3181 cgggcggcac gccgggcagc agcggcggga cgtcgctcgg gctcgtgtgg aacatggggt
     3241 gctgctgatc ctgcatgtag ttgtacttga gctcggtctc gtaagccgcc tggtgcgcgt
     3301 cgcgcatgat ctgcgggcga atgaagatca tcaggttgtt cttggtggag ctctgattgc
     3361 gtgtcttgaa gagcagtccg aggatcggga tgctgccgag gtagggtact ctgctctcac
     3421 ccttggacat gtcgtcc
//
LOCUS       DATDMP010000384         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1616999, whole genome shotgun sequence.
ACCESSION   DATDMP010000384 DATDMP010000000
VERSION     DATDMP010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616999"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VI195_06685"
     CDS             <1..673
                     /locus_tag="VI195_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824109.1"
                     /translation="SGARVDEAISWRGLWELMRNRDILALTASYICMNYVFYLLANWS
                     FLYLVQERHFTRLEGGWLASTPPLAAAIGAGIGGQLASVLGERYGVRTGLRIIPLLTL
                     PAAGVLQFAAVDATNAYLAVVALALCFAAVEMNEGPYWAAIMHVGRADSMAASGLLNT
                     GGNLGGLICTPIVAYLSGHHAWKLAFLIGMAFAAVSAALWLVLDPSRAARVTTAAAVP
                     QPSRA"
     gene            complement(805..>2220)
                     /locus_tag="VI195_06690"
     CDS             complement(805..>2220)
                     /locus_tag="VI195_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6824110.1"
                     /translation="GLYGDSYGTYFAQVFALRHPERLRTLVLDGAYPLSGPDYPWYPN
                     IAPAMRRKFDVACERAPGCRAIPGSSMEHILPALGELRARPFEANVRFGEGHPVKFTA
                     DASALAILMYAGSPAEASVRELDAAARAFSGGDRLPLLRLMAETLGSVDSRDPSGDPV
                     KFSAGLAAAIWCEDPPHIFDMHLAPEERVAERDRIIAERKVSAPDTYAPFTIDEYRRM
                     PLDYAYIDQCVRWPAAAPESRPLTFEASRYPPVPVLVISGEFDDQTSVADGEAAAARY
                     PHARHIVIANSFHVNALPGARSECAAGLVRRFMEDLTPGDASCAAAVPPVPLVAHFAR
                     YRHELAPAEPIARNEADEEALRAVSAALLTSADVITRAAENGAGWGLGLRGGTFTAAT
                     QGEGYRITLQQVRWTEDLTASGRIDWPGRSGVVHAELEVHSPQESGRLDLSWPQGAPG
                     ARASARGTLGGKSVAAEAPTP"
BASE COUNT          346 a          756 c          742 g          376 t
ORIGIN      
        1 aagcggagcg cgtgtcgatg aggctatttc atggcgcgga ttgtgggagc tgatgcgcaa
       61 ccgcgacatc ctcgccctca ccgcctccta catctgcatg aactacgtgt tctatctgct
      121 cgccaactgg tccttcctct atctggttca ggagcgtcac ttcacacgcc tcgagggcgg
      181 ctggcttgcg agcacaccgc cgctcgccgc ggcgatcggt gccggtatcg gcgggcagct
      241 cgccagcgtg ctcggcgagc gctacggcgt gcgcaccggg ttacggatca ttccgctgct
      301 cacgctgcca gctgccggag tactgcagtt cgccgccgtg gatgccacca acgcctacct
      361 ggcggtcgtg gcgctggcac tgtgttttgc cgcggtggaa atgaacgaag gcccgtattg
      421 ggccgccatc atgcacgtcg gacgggcgga ttcgatggcg gcatccgggc tgctgaacac
      481 cggcggcaac ctgggcggac tcatctgtac gccgatcgtc gcgtatctct ccggccatca
      541 cgcctggaag ctggctttcc tgatcggcat ggcctttgcg gctgtgagtg ccgcgctctg
      601 gttggtcctg gaccccagtc gcgccgcacg cgtgaccacg gccgcggcgg tgccccagcc
      661 ctcgcgcgct taatggatgc cgcaaggatg gttaaccaaa tggtgaactg tctaactaac
      721 gaggtttaac catccggcca acaccaagcg accgcagttg gtcagctccg ggtccgtggc
      781 cgcggtcaat ggccgccgac tcgctcaggg cgtcggggcc tccgcagcga cgcttttgcc
      841 accgagggtc cctcgagcac tggcgcgtgc gcccggcgct ccctggggcc aggagagatc
      901 cagccggccg ctctcctgcg gggagtgcac ctcgagctcc gcgtgcacca ctccgcttcg
      961 gcccggccag tcgatgcgac ccgaggcggt gaggtcctcg gtccagcgca cctgctgcag
     1021 cgttattcga taaccctcgc cctgtgtcgc ggcggtgaac gtcccgccac gcagccccag
     1081 accccatccc gcaccgtttt cggcggcgcg agtgatcaca tccgcagagg tgaggagtgc
     1141 ggcgctcacc gcccgcagcg cctcttcgtc cgcctcattt cgagcaatcg gctcggccgg
     1201 cgccagctcg tgccggtatc gggcgaagtg cgcgacgagc ggcaccggag gcaccgcggc
     1261 cgcgcagctc gcatcgccgg gcgtcaggtc ctccatgaag cgccgcacca gtcccgcggc
     1321 gcactcgctg cgggcccccg gaagtgcgtt cacatgaaag ctgttggcga tgacgatgtg
     1381 gcgcgcgtgc ggatagcgcg ccgccgcagc ttcgccgtcc gccacgctcg tctgatcatc
     1441 gaactcaccg gaaatcacca gcaccggaac cggcggatag cgtgacgctt cgaacgtgag
     1501 cggtcggctc tccggtgccg ccgccggcca gcgtacgcac tgatcgatgt aggcatagtc
     1561 gagcggcatg cggcggtact catcgatggt gaagggtgca taggtgtcgg gcgcgctcac
     1621 cttgcgctcg gcaatgatgc ggtcacgttc ggcgacccgt tcctccggcg ctaggtgcat
     1681 gtcgaagatg tgcggcgggt cctcgcacca gatcgccgct gcgagtccgg cactgaattt
     1741 cacgggatcg ccgctcggat cgcgcgaatc gacactgccg agtgtctctg ccatgaggcg
     1801 cagcaggggc agccggtcgc cgccgctgaa ggcacgcgcc gcggcatcga gctcgcgcac
     1861 gctcgcctca gccggagagc cggcgtacat gaggatcgcg agcgcactgg cgtcggccgt
     1921 gaacttcacg gggtggccct cgccgaagcg cacgttggcc tcgaacggcc gggcgcgcag
     1981 ctctccgagt gccgggagga tgtgctccat cgagcttccc ggaatggccc ggcagccggg
     2041 cgcacgctcg cacgccacat cgaacttgcg gcgcatcgcc ggtgcaatgt tggggtacca
     2101 cgggtagtcg ggtccggaga gcggatacgc cccatcgagc acgagggtcc ggagacgctc
     2161 gggatgacgt agcgcgaaaa cctgtgcgaa ataggtcccg taggagtcgc cataaaggcc
//
LOCUS       DATDMP010000385         3783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1839842, whole genome shotgun sequence.
ACCESSION   DATDMP010000385 DATDMP010000000
VERSION     DATDMP010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839842"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VI195_06695"
     CDS             complement(<1..1663)
                     /locus_tag="VI195_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6824111.1"
                     /translation="MKTIPSLLIAAVFATLTFAVWAYLNRPTREPPWPSLIQGFDFSP
                     FRAGEDPTHFEMPTDAEIDADLTLLEGKVKAVRTYSVGATLADVPELAERHGLNVALG
                     AWLDTHRDKNEEQLKTAIELANTHQNVVRVIIGNEVLLRGDLAVEELEKYLDRARDAV
                     SQPVGTAETWHTWLVHPELAQHVDFIGVHLLPYWEGVTVDKAVDYSLAQFKRLQKTFP
                     HKPIVVAEIGWPSRGRTHESAVASVANEALFLRRFLYRAQKEQTTYYVMEAFDQPWKA
                     FMEGAVGSYWGVYDVERRPKFAFTAPIVRIPEWHILAAVSIAVSLLVLGLLYLNSDTL
                     RNRGRSFLAVVVYAAATIAVWVIYDFTQQYMTVSSVVSGVLLLLGMLGVLLVLLAEAH
                     EWAEAHWVEYRRRLGAPIPTHSAAPPKVSVHVPAYNEPPAMLIETLDALARLDYPDYE
                     VLVIDNNTRDESVWRPVEAHCAKLGARFRFFHVAPLAGFKAGALNYALERTAPDAAIV
                     AVIDSDYVVDARWLHDLTPAFQDARIAIVQAPQDYRDADQSAFKAM"
     gene            complement(1682..2101)
                     /locus_tag="VI195_06700"
     CDS             complement(1682..2101)
                     /locus_tag="VI195_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6824112.1"
                     /translation="MKYLHTMVRVTDLDASLRFYCDALGLKELSRRDYPQGRFTLVFL
                     AAPGDESAQVELTHNWDPEKYTGGRNFGHLAYAVDDIYAACQRLRDRGVTILRPPRDG
                     HMAFVRSPDDISIELLQKGAALAPAEPWKSMANTGSW"
     gene            complement(2134..>3783)
                     /locus_tag="VI195_06705"
     CDS             complement(2134..>3783)
                     /locus_tag="VI195_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6824113.1"
                     /translation="VPMNAPPEMLGVLARALLKAPGHRLALLPAGEATIEAVAASPAQ
                     PGAAGVDALTQYQISGLDFSPTPIWLDREGETAAIASSWLSVLTPASRERLPQLLAAQ
                     QQANDAWSARLARAQTLVPAEPVVIRNARLFDPRDLSVTAGSSVLIRGERIVQVADDA
                     ALPVPAGARVIEAGGRFLMPGLWDNHQHFGDNVGVLDIATGVTSARDMANDTDEFLER
                     VTRFDAGTELGPRVWKAGIIDGAGPLAGPTKMRISNAAEAVQDVDWYADHGYGQIKIY
                     SSVPPAIVPVIAAEAHAHGLRVSGHVPAFMSAHQFVAGGADEIQHLNFIVLDFLFDQV
                     KETRNMNRFTAVAAHAREFTPEKPPVQELIGYLKHHHTVLDPTVNVFEAMFCGNPAAV
                     TPGLEQIAPRMPAQVRRGMLSGALVVPQGEEAAYREALPALLALLKALYDAGITIIPG
                     TDSFAGYGLHHELELYARAGIPPPEVLRLATLTSAQVIGVDGERGVVAPGKLADLILV
                     EGDPSAHIEDIERVSLVMRGGRIYDPAHIEQALGIAPRPSG"
BASE COUNT          638 a         1282 c         1263 g          600 t
ORIGIN      
        1 acatcgcctt gaaggcgctc tggtcggcat cgcgataatc ctgcggcgcc tgcacgatgg
       61 cgatgcgcgc atcctggaat gccggggtga gatcgtgcag ccagcgggca tcgaccacgt
      121 agtcgctgtc gatgacggcg acgatggcgg cgtcgggcgc ggtgcgctcc agggcgtagt
      181 tgagcgcgcc ggccttgaag ccggcgagcg gcgcgacatg gaagaagcgg aagcgcgcgc
      241 cgagcttggc gcaatgcgct tccaccggcc gccacacgct ctcatcgcgc gtgttgttgt
      301 cgatcaccag cacctcgtag tccggatagt cgagccttgc cagcgcatcg agcgtctcga
      361 tgagcatcgc gggcggctcg ttgtaagccg gcacgtggac ggagaccttg gggggcgcgg
      421 cgctgtgagt cgggatcggc gccccgaggc gccgccggta ttccacccag tgcgcctcgg
      481 cccactcgtg tgcttcagcg agcagcacca gcagcacgcc gagcatgccg agcagcagca
      541 gcacgcccga caccacgctc gagacggtca tgtactgttg cgtgaaatcg tagatgaccc
      601 acacggcgat ggtggccgcg gcatacacca ccaccgcgag gaagctgcgg ccgcgattgc
      661 gcagcgtgtc gctgttcaga tagagcagcc cgagcaccag cagcgacacc gcgatcgaga
      721 cggcggcgag aatgtgccac tcgggaatgc gcacgatcgg cgcggtgaac gcgaacttgg
      781 gccggcgctc gacgtcgtac accccccagt acgagcccac cgccccttcc atgaacgcct
      841 tccagggctg atcgaaggcc tccatcacgt agtaggtggt ctgctccttc tgtgcgcgat
      901 acaggaagcg gcgcaggaac agcgcctcgt tcgccaccga ggcaaccgcc gactcgtgcg
      961 tgcggccgcg gctcggccag ccgatctccg ccaccacgat cggcttgtgc ggaaaggtct
     1021 tctgcaggcg cttgaactgc gcgagcgaat agtcgaccgc cttgtcgacc gtgacgccct
     1081 cccagtaggg cagcagatgc acaccgatga aatcgacgtg ctgcgccagc tccgggtgta
     1141 ccagccaggt gtgccaggtc tcggcggtgc cgaccggctg cgagaccgca tcgcgagcgc
     1201 gatcgagata cttctccagc tcctctacgg ccaaatcgcc gcgcagcagc acctcgttgc
     1261 cgatgatgac gcgcacgacg ttctgatgag tgttggcgag ctcgatggcg gtcttcagct
     1321 gctcctcgtt cttgtcgcgg tgcgtgtcga gccaggcgcc gagcgcgacg ttcaggccgt
     1381 gccgctcggc cagctccggc acgtccgcca gcgtggcgcc gacgctgtag gtgcgcaccg
     1441 ccttaacctt gccttcgagc agcgtcaggt ccgcatcgat ctccgcgtcg gtgggcatct
     1501 cgaagtgcgt cggatcctcg ccggcgcgaa acggcgagaa atcgaacccc tggatcagcg
     1561 acggccaggg cggctcgcgc gtcggacggt tgaggtaagc ccacaccgca aaggtcagcg
     1621 tggcgaacac cgccgcgatc agcaggctcg gaatggtttt cacgtgcccc cgtgcgcgcg
     1681 cctaccagct gcccgtgttg gccatggact tccagggctc cgccggcgcc agcgctgcgc
     1741 ccttctgcaa cagctcgatc gagatgtcat ccggtgaacg cacgaacgcc atgtgtccat
     1801 cgcgcggtgg ccgcaggatg gtgacgcccc gatctctcaa gcgctggcag gccgcgtaga
     1861 tgtcgtccac cgcgtaagcg agatgcccga agttacgccc gcccgtgtat ttctcgggat
     1921 cccagttgtg agtgagctcg acctgcgcac tctcatcacc cggcgccgcg agaaacacga
     1981 gcgtgaagcg cccctgcggg tagtccctgc gcgagagctc cttcaggccg agggcgtcgc
     2041 agtagaacct cagcgacgca tcgaggtcgg tgacacgaac catggtgtgc aggtacttca
     2101 tagagcctct cgcatgaacg gaccgtgaac gcttcagcca ctcgggcgcg gtgcgatgcc
     2161 gagcgcctgc tcgatgtgcg ccgggtcgta gatccggccg ccgcgcatga ccagcgacac
     2221 ccgctcgatg tcctcgatgt gagcgctcgg gtcgccctcc accagaatga gatcggcgag
     2281 cttgccgggt gccaccacgc cacgctcacc atcgacaccg atcacctgtg ccgaggtcaa
     2341 tgtagcgagg cgcagcacct cgggcggcgg aatgccggcg cgcgcataca gctcgagctc
     2401 gtgatgcagc ccgtagccgg cgaaggaatc ggtgccgggg atgatggtga tgccggcatc
     2461 gtagagcgcc ttgagcagcg caagcagcgc gggcaaagcc tcgcgatacg ccgcctcctc
     2521 gccctgcggc accaccagtg cgcccgagag catgccgcgc cgcacctgag ccggcatgcg
     2581 tggggcgatc tgttcgagcc ccggagtcac cgccgccggg ttgccgcaga acatggcctc
     2641 gaacacgttg accgtcgggt cgagcaccgt gtgatggtgc ttcaggtagc cgatcagctc
     2701 ctgcaccgga ggcttctcgg gggtgaactc gcgggcgtgc gcggcaaccg ccgtgaagcg
     2761 gttcatgttg cgggtctctt tcacctgatc gaagaggaaa tcgagcacga tgaagttgag
     2821 gtgctggatc tcatcggcgc cgccggccac gaactgatgc gcgctcatga acgccggcac
     2881 gtgcccactc acgcgcaagc catgcgcgtg cgcctcggcg gcgatcaccg gcacgatcgc
     2941 cggcgggacc gaggagtaga tcttgatctg cccgtagcca tgatcggcat accagtcgac
     3001 gtcctgcacc gcctctgcgg cgttgctgat gcgcatcttg gtggggcccg ccaacggccc
     3061 ggcaccgtcg atgatgccgg ccttccacac ccgcggcccg agctcggtgc ccgcatcgaa
     3121 gcgtgtgaca cgctcgagga attcgtcggt gtcattcgcc atgtcgcgcg cgctcgtcac
     3181 gccggtggcg atgtcgagca cgccgacgtt gtcgccgaag tgctgatgat tgtcccagag
     3241 ccccggcatg aggaagcgtc cgcccgcctc gatgacccgc gcgcccgccg gcacgggcag
     3301 tgcggcatcg tcagcgacct gcacgatgcg ctcgccacgg atcagcacgc tgctgcccgc
     3361 agtgacgctc agatcgcgcg gatcgaagag ccgcgcattg cggattacga ccggctcggc
     3421 cggcaccagt gtttgtgccc tggcgaggcg cgcggaccac gcatcattcg cctgctgctg
     3481 tgccgcgagc agctgcggca ggcgctcgcg cgaggcgggc gtgagcaccg agagccagct
     3541 ggaggcgatc gcagccgtct caccctcgcg atcgagccaa atcggcgtcg gtgaaaaatc
     3601 gaggccgctg atctggtact gcgtgagcgc atcgaccccg gccgcgcccg gctgtgcggg
     3661 cgacgccgcc accgcttcga tggtcgcctc gcccgccggc agcagtgcca ggcgatgccc
     3721 gggagccttg agaagcgcgc gcgccagaac cccgagcatc tccgggggag cattcatggg
     3781 gac
//
LOCUS       DATDMP010000386         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_396094, whole genome shotgun sequence.
ACCESSION   DATDMP010000386 DATDMP010000000
VERSION     DATDMP010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396094"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VI195_06710"
     CDS             complement(<1..922)
                     /locus_tag="VI195_06710"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY6824114.1"
                     /translation="MRRQWRALGAWTVALLLGAAVIPGRAQDADPPGRAARLSDAEGA
                     VSLQPAGVQEWTAATVNRPVTTGDRLWSDQSSRAELDLGTAAVRLGSNTGFAFLNLDD
                     RSVQMQLSAGALIVSVRDILNGESYEVDTPNVALSLQQPGVYRVEVNDQGTATAVAIS
                     QGAAQVAGGGQTITIGSQQAVTFTGADSLAWQSTTLGAPDEFDDWSALREREAAQSAS
                     AGYVASDIPGTQDLDSNGQWQQTPEYGYVWEPTVVAVGWVPYRYGQWLWVTPWGWTWV
                     DDARWGYAPFHYGRWVVWNHNWCWVPAPKHG"
     gene            complement(1054..1557)
                     /locus_tag="VI195_06715"
     CDS             complement(1054..1557)
                     /locus_tag="VI195_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824115.1"
                     /translation="MVRAALRRPEVNGSGQGSIELSTAIAARSTLPWLLRAAIYAVAA
                     LLWLSGALWLGLHHAFPQQSAFGPLPNPLEAPLMRVHGLIAVGGVFLIGWMTAAHVTA
                     RWSSERNRRSGFALGVTALLLVFSGYALYYTTGSPHDSAGILHEAIGLLSPAAALAHW
                     WRTRSRA"
     gene            complement(1561..2139)
                     /locus_tag="VI195_06720"
     CDS             complement(1561..2139)
                     /locus_tag="VI195_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY6824116.1"
                     /translation="MSGINRLMPGWIGALGVLGVGAPIQVSVATEYLSVEGAQKALFP
                     QADHFTEVVLALSSTQRERVAALAGPQPPHRSLRAWRALRGDELLGFLFVDEVIGKED
                     FITYAVAVDARGRLSAPEVLAYRESHGGEIRSEAWRRQFAGRHGLDQLHVQTDIKNIA
                     GATLSCEHVTEGVRWVTALWQVALQPAPAALG"
     gene            2294..>2492
                     /locus_tag="VI195_06725"
     CDS             2294..>2492
                     /locus_tag="VI195_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824117.1"
                     /translation="MAVEAAAETQQAALAGVEAAYLAIQCIERRLHPSREGSELQRIR
                     GAPLDTPVRIAGMTWQLLHLAQ"
BASE COUNT          445 a          842 c          803 g          402 t
ORIGIN      
        1 tgccatgttt gggggcgggc acccagcacc agttgtggtt ccacacgacc cagcggccgt
       61 aatgaaaggg ggcgtaaccc cagcgggcat catcgaccca cgtccacccc cacggagtga
      121 cccacaacca ttgaccgtac cggtacggta cccaacccac cgccacgacc gtcggctccc
      181 agacataacc atactcgggg gtctgctgcc attggccgtt gctgtcgagg tcctgcgtac
      241 cgggtatatc acttgcgacg tagcctgcgg aggctgactg cgcggcctcg cgctcgcgca
      301 gcgcggacca gtcatcgaat tcatctggtg cgccaagtgt ggtgctctgc cacgccagtg
      361 agtcggcacc cgtgaaggtc accgcctgtt gcgagccgat ggtgatggtc tgaccgccgc
      421 cggccacctg cgccgcaccc tggctgatgg caaccgcggt tgcggttcct tggtcattga
      481 cctcgacgcg gtacacgccc ggctgctgca aggacaatgc aacgttgggt gtgtcgactt
      541 cgtagctctc gccattgagt atgtcgcgca cactcacgat gagcgcaccg gcgctcagct
      601 gcatctgcac gctgcgatca tcgaggttga gaaaggcaaa gccggtgttg ctgccgagcc
      661 tgaccgcggc ggtgccgaga tcgagctcgg cgcgtgagct ttgatcgctc cacagtcgat
      721 caccggtggt gactggacga ttgactgtcg cggcggtcca ttcctgcact ccagccggct
      781 gcagcgacac ggcgccctcg gcatcgctca gccgtgcggc gcgtcccggg ggatcggcgt
      841 cttgcgcacg cccaggtatg accgcggcac cgagcagcag cgccacagtc caggcaccga
      901 gtgcgcgcca ttgccgtctc atcagtcgca tccgcctcac ctgaaggcac aacgctcgag
      961 cttccggcgg ggatgacccg cgggtgcctt cacagtttag tgggtcgcag agaaagcgtc
     1021 cccccgggcc ggcgcgactg cgggaacgct gcctcaggcc ctcgagcggg tgcgccacca
     1081 gtgagcgagt gccgctgcgg gggacaacaa tccgatcgcc tcgtggagga tgccggccga
     1141 atcgtgcggt gagccggtgg tgtaataaag cgcatagccg ctgaagacca ggagcaacgc
     1201 tgtaacgccg agcgcaaagc cagagcggcg attgcgttcg ctcgaccagc gcgcggtcac
     1261 atgcgccgca gtcatccaac cgatgaggaa tacgccaccg accgcgatga gaccgtgtac
     1321 gcgcatcagc ggcgcttcca atggattggg taacggcccg aatgcgcttt gctgcgggaa
     1381 cgcatgatgc agcccgagcc agagtgcacc cgagagccac agcagcgcgg ccacggcata
     1441 gatcgccgcg cgcagcagcc agggcagcgt tgagcgtgcc gcaatcgcag tgctcagctc
     1501 tatcgagccc tgtcccgagc cattgacttc cggtcgacgc aaagccgcgc gcaccatgag
     1561 ttaccccagc gcggcaggcg caggctggag cgctacctgc cacagcgccg tgacccagcg
     1621 caccccttcg gtcacgtgct cacaggagag cgtcgcgccc gcgatgttct tgatgtcggt
     1681 ctgcacgtgc agctgatcga gcccgtggcg cccggcgaac tggcgccgcc aagcctcgct
     1741 gcgaatctct ccgccatgac tttcacggta ggcgagaact tccggcgcac tgagccggcc
     1801 gcgcgcatcg acggcgaccg cgtaggtgat gaagtcctct ttgccgatta cctcgtcgac
     1861 gaataggaat ccgagcagct catctccgcg cagggcgcgc catgcccgca ggctccggtg
     1921 gggcggctga ggcccggcaa gcgcggcgac ccgctcccgc tgcgtggaac tgagcgcgag
     1981 aaccacctcg gtgaagtgat cggcctgtgg aaagagcgcc ttctgcgcgc cttccaccga
     2041 gaggtattcc gtggccaccg acacctggat gggcgcaccc acgccgagca cgccgagcgc
     2101 gccgatccac cccggcatca agcggttgat cccggacata cccgccatcc acaaatgcga
     2161 atgattctta attgctttag aataacccta acgggtgcag aaggtccagg ccgtgggttt
     2221 tatggagttg gcagggcgga tccgcaggtc cgcggctcac gtgcagcgtc ttcggcccgg
     2281 gatgggcact tgggtggcgg tcgaggccgc cgccgagacc cagcaggcgg cgctggcggg
     2341 cgtggaagct gcctatctcg caatccaatg catcgagcgg cggctgcacc caagccgcga
     2401 aggcagcgag ctccagcgca tccgcggcgc tccgctcgac acccccgtcc gtatcgcggg
     2461 gatgacctgg caattgctgc acctggcgca at
//
LOCUS       DATDMP010000387         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_730020, whole genome shotgun sequence.
ACCESSION   DATDMP010000387 DATDMP010000000
VERSION     DATDMP010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730020"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VI195_06730"
     CDS             complement(<1..304)
                     /locus_tag="VI195_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6824118.1"
                     /translation="MKAVQKGFTLIELMIVVAIIGILAAIAIPLYGSVMTRARIAKAQ
                     ADVRTLASAVSIYGAHMGVLPAALDDLTSQVTNPQNQVGGPFMASVPTPPAGWSSYG"
     gene            complement(555..1949)
                     /locus_tag="VI195_06735"
     CDS             complement(555..1949)
                     /locus_tag="VI195_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6824119.1"
                     /translation="MSEAVSANRTAAGQPVVLVVDDEPDLLELVSLTLGRMSLRTRTA
                     ADIGSARRLLKAERFDLCLTDMRLPDGDGLELVAWIQEHRASVPVAVITAHGNVESAV
                     RALKLGAFDFVSKPLDLGVLRKLVGSAIRLASAAEDTASKLRGPRLLGSSAAMEQLRE
                     MIARVARSQAPVHICGESGTGKELVARMIHESGPRRGGPFVAVNCGAIPTELMESELF
                     GHKRGSFTGAVADKKGLVQSAEGGTLFLDEVADLPLHMQVKLLRVVQEKTVRPVGEAR
                     EETVDVRILSATHKTLADLVAQGLFREDLFYRINVIELRVPALRERSSDIREIAAAIL
                     ERLARRASGPATQITDAAMKLLEAYPFPGNVRELENVLERALTLSTGGMITPEHIRLR
                     VANRSPDPEAVTPGITAPLAVDGALGSQLESIEREAILKALEKTRYNKTAAAKLLGMS
                     FRALRYRIKKLGIE"
     gene            complement(1946..>2228)
                     /locus_tag="VI195_06740"
     CDS             complement(1946..>2228)
                     /locus_tag="VI195_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6824120.1"
                     /translation="ESADQILEIRYGRMSGSDRPFLEVADRGTGVAPEHAERIFEPFY
                     SRGRGTGLGLFLARELAQANGATLLHEPRPGGGSVFRLVFADPRRWEIR"
BASE COUNT          392 a          734 c          712 g          390 t
ORIGIN      
        1 aaccatacga ggaccagccc gcgggcggcg tcggaaccga cgccatgaac ggcccgccca
       61 cctggttttg tgggttcgtc acctggctgg tgaggtcgtc cagagccgcc ggcagcacac
      121 ccatgtgagc gccgtagatc gagacggccg acgcgagcgt acgaacatcg gcctgcgctt
      181 tcgcaatacg cgcacgcgtc atgacgctgc catacagcgg gatcgcaatg gcggccagaa
      241 tgccgatgat cgccaccacg atcatcaact cgatcagggt aaaacccttt tgcacagcct
      301 tcatttgaaa aaactcctaa aagctttggg tacaacgtca gcggcgcaaa aggcgccccg
      361 gctgggtaac agcaaggctt gtgccagagc gtcagaatag aggcaaggca ttgatttaaa
      421 atgaatctct gcccattaga cgcagcggat actttactta cttttgtcgc agactgacgc
      481 caccggttgg ccgccgcgtc aggaagtgac gtaaatcgtc atatctccgc aggggatcgg
      541 acggctgccc ttcactactc gatccccagc ttcttgatcc gatagcgcag tgcccggaag
      601 ctcattccaa gaagcttcgc tgcggcggtc ttgttgtagc gggtcttctc gagggccttg
      661 aggatggctt cgcgctcgat gctctcgagc tgactgccga gagccccgtc caccgcaaga
      721 ggagccgtaa tgccgggtgt gaccgcctcc gggtcaggcg agcgattcgc tacccgcagg
      781 cggatgtgct cgggtgtgat catcccgccg gtgctgagcg tgagagccct ctcgagcaca
      841 ttctcgagct cgcgcacgtt gccggggaag ggataggcct cgagtagctt cattgcggcg
      901 tccgtgatct gcgttgccgg gccgctggcg cgccgtgcga gtcgctcgag gatcgccgcc
      961 gcgatttcac ggatatcgct gctgcgctcg cgcagcgccg ggacgcgtaa ttcgatgacg
     1021 ttgatcctgt aaaagaggtc ctcgcggaag agtccctggg cgacgaggtc ggccagggtc
     1081 ttgtgagtcg cggacaggat gcgcacatcg acggtctctt cgcgcgcctc gccgaccgga
     1141 cgcacggtct tttcctgcac gacccgcagc agcttcacct gcatgtgcag cggcagatcc
     1201 gccacctcat cgagaaatag cgtgccgccc tcggcgctct gcaccagtcc cttcttgtcg
     1261 gccactgcgc ccgtgaagct gccgcgcttg tgaccgaaca gctcgctctc catgagctcg
     1321 gtcgggatgg cgccgcagtt cactgcgacg aacggtccgc cgcgccgcgg gccgctttcg
     1381 tggatcatgc gcgcaacgag ctccttgccg gtgccggatt cgccgcagat gtgcaccggg
     1441 gcctggctgc gggcgacgcg ggcgatcatc tcgcgcagct gttccatcgc cgccgagctg
     1501 ccgagcagcc gtgggccgcg cagtttggaa gccgtgtcct cggccgcgga ggcgaggcga
     1561 atggcgctgc cgacgagctt gcgcagcaca ccgaggtcga gcggtttcga cacgaaatcg
     1621 aacgcgccga gcttcagcgc ccgcaccgcc gactcgacat ttccgtgcgc cgtgatcacg
     1681 gcaaccggca ccgacgcccg gtgttcctgg atccaggcca ccagctcgag gccatcgcca
     1741 tcgggcagcc gcatgtcggt gagacagaga tcgaagcgct ccgctttgag taagcggcgc
     1801 gcgctgccga tatcggcggc ggtgcgagtg cgcaggctca tgcggccgag tgtgaggctg
     1861 accagctcga gcagatccgg ctcatcgtcc accaccagca ccacgggttg cccggcggca
     1921 gtgcgattgg cgctcacggc ttcgctcatc ggatctccca gcggcgcgga tcggcgaaca
     1981 ccaggcggaa tacgcttccg ccaccggggc gaggctcgtg cagcaaggtg gcgccgttgg
     2041 cctgcgcgag ctcgcgcgcg agaaacagcc cgagccccgt gccacgtccg cggctgtaga
     2101 aaggctcgaa aatccgctcc gcatgctcgg gcgccacacc tgtgccgcgg tcggccacct
     2161 ccaggaacgg ccgatcgctg ccactcatgc gtccgtagcg gatctcgaga atctgatccg
     2221 cggactcc
//
LOCUS       DATDMP010000388         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1840243, whole genome shotgun sequence.
ACCESSION   DATDMP010000388 DATDMP010000000
VERSION     DATDMP010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840243"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VI195_06745"
     CDS             complement(<1..1788)
                     /locus_tag="VI195_06745"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6824121.1"
                     /translation="MRRRSCIRTLLVAAMAALLAAAGADARRHDPEKLSEGHIRDLHF
                     GDVLFLYFQQDDAHDFEALTRVLAYQHWGRMPHHAEDAELIAGSLYLQEGMHNEAGEI
                     FQRLLTDNVSSGVRNRAWLYLAQVWYARGYYPKAEGALRKVNGRMSPDFEAQKELLLA
                     NVLMHQGKYDEAIQLLTGWRGGPVWSAYARFNLGVALVRANRLADADPFLSAVGTMVA
                     PNPELAALRDRANLALGFAYLQANQPERARAPLERVRLNGPYSNKALLGTGWAQVARG
                     DYQGALTPWMELRGRDVLDAAVQESYLAVPYAFMKMNANAQSAEYYESAVTSFGSETT
                     RLDAAIAHIRQGDMLREVLASGPAHTASDAPLLHGWFRDLRNLPRTAEAPYLYSVLAG
                     HDFQEGVKNYRDLVYLGSTLEHWGDSMDAYQDMIDTRERAYAERLPRVDKLLASGTLQ
                     ELQQRQVALENRLHTIEAQHDVAALGTETERSQWARIQRVEGSLAGVPESPDSTDERA
                     RLALVKGVMYYRLDEAFGARVWQEHRSLKDLALALHEAQSRWIRVERARHNVPQNNGE
                     FATRVASLRERIAALQVRLAATEERQSAYL"
     gene            complement(1793..>2081)
                     /locus_tag="VI195_06750"
     CDS             complement(1793..>2081)
                     /locus_tag="VI195_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824122.1"
                     /translation="VQLRLDQKEVINYLYTPRERDALLKGGVQRLYLGNLKVGSHELV
                     AFFNGSGPNSRPYKRGASLRFEKGVGAKYLELKINDRQRKLEPEFEIKDWE"
BASE COUNT          310 a          707 c          699 g          365 t
ORIGIN      
        1 caggtaggca ctctggcgct cctcggtcgc ggcgagacgc acctgcagcg ccgcgatgcg
       61 ctcgcgcagg ctggcgacgc gcgtggcaaa ttcgccgttg ttctgcggca cgttgtggcg
      121 cgcgcgctcc actcttatcc agcggctctg cgcctcgtgc agcgccagcg cgagatcctt
      181 gaggctgcgg tgctcctgcc agacgcgcgc gccaaaggcc tcgtccaggc ggtaatacat
      241 cacgcccttc accagcgcca ggcgtgcgcg ctcatccgtg ctgtccgggg actcgggaac
      301 tcctgcgaga ctgccctcca cgcgctggat gcgcgcccat tgcgagcgct ccgtttcggt
      361 gccgagcgcg gccacatcgt gctgcgcctc gatggtgtgc aatcgattct cgagcgccac
      421 ctggcgctgc tgcagctctt gcagcgtgcc ggaggcgagt aacttgtcca cccgtgggag
      481 ccgctcggcg taggcgcgct cgcgcgtgtc aatcatgtcc tggtaggcgt ccatgctgtc
      541 gccccagtgc tcgagcgtac tgccgaggta gaccaggtca cggtagttct tgacgccttc
      601 ctggaagtcg tgccccgcga gcacgctgta gaggtacggt gcctcggcgg tgcgcggcag
      661 gttgcgcagg tcgcggaacc agccgtgcag cagcggcgcg tcgctggccg tgtgtgccgg
      721 gccgctcgcg agcacctcgc gcagcatgtc accctggcgg atgtgcgcga tcgcggcatc
      781 gagccgcgtg gtctcgctgc cgaaagaggt gaccgcactc tcgtagtact ccgccgactg
      841 cgcgttggca ttcatcttca tgaacgcata cggcacggcg aggtacgact cctgcaccgc
      901 cgcatcgagc acgtcgcggc cgcgcagctc catccacggg gtgagcgcgc cctgataatc
      961 tccccgcgcc acctgcgccc agccggtgcc gagcagcgcc ttgttggagt aaggaccatt
     1021 cagccgcacg cgctcgagcg gtgcgcgcgc ccgctccggc tggttcgcct gcaggtaggc
     1081 gaagccgagc gcgaggttgg cacggtccct gagcgcggcg agctccgggt tgggtgcgac
     1141 catggtgccg accgcggaga ggaacgggtc ggcgtcggcg aggcggttgg cgcgcaccag
     1201 cgcaaccccg agattgaagc gcgcgtacgc cgaccacacc ggtccgccgc gccaacccgt
     1261 caacagctgg atcgcctcgt cgtacttgcc ctggtgcatg agcacgttgg cgagcagcag
     1321 ctccttctgc gcttcgaagt ccggcgacat gcgcccgttc accttgcgca gcgcgccctc
     1381 ggccttcggg tagtagccgc gcgcgtacca cacctgcgcc agatacagcc aggcgcggtt
     1441 gcgcactccc gaggagacgt tgtcggtcaa caggcgctgg aagatctcgc ccgcctcgtt
     1501 gtgcatgccc tcctgcagat agagtgagcc ggcaatgagc tcggcatcct cggcgtggtg
     1561 gggcatgcgt ccccagtgct ggtaggcgag cacgcgcgtc agcgcctcga agtcgtgcgc
     1621 gtcgtcctgc tggaagtaca ggaacagcac atcgccgaag tgcaggtcgc gaatgtggcc
     1681 ttcggagagc ttttccggat cgtgccggcg cgcgtcggcg cctgccgcgg cgagcaacgc
     1741 cgccatggca gccacgagca gcgtgcgtat gcaggaccgg cgcctcaagg gcctattccc
     1801 agtccttgat ctcgaactcc ggctcgagct tgcgttgccg gtcgttgatc ttgagctcga
     1861 gatacttcgc gccgacgcct ttctcgaagc gcagcgaggc gccgcgcttg taggggcgcg
     1921 agttaggtcc cgagccgttg aaaaacgcca ccagctcgtg cgagccgacc ttgagattgc
     1981 cgaggtacag gcgctgcacg ccgcctttga ggagcgcatc gcgctcacgc ggtgtgtaga
     2041 gatagttgat cacttccttc tgatcgagtc tcagctgcac c
//
LOCUS       DATDMP010000389         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_174450, whole genome shotgun sequence.
ACCESSION   DATDMP010000389 DATDMP010000000
VERSION     DATDMP010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174450"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /gene="recR"
                     /locus_tag="VI195_06755"
     CDS             complement(<1..458)
                     /gene="recR"
                     /locus_tag="VI195_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY6824123.1"
                     /translation="MAAPLDKPAALEELIQALRCLPGVGPKSAQRMAYHLLQRDQKGA
                     GRLGAALSGALERIRHCARCNTFTESEVCALCASPRRDASLLCVVETPGDLLMMEQTQ
                     SYPGLYFVLLGRLSPLDGIGPREIHLDRLARRATDGAVKEVILATNFTVEG"
     gene            complement(459..785)
                     /locus_tag="VI195_06760"
     CDS             complement(459..785)
                     /locus_tag="VI195_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY6824124.1"
                     /translation="MMKGGLGALMKQAQQMQENMRKAQEELANIDVEGQSGAGLVKVA
                     MSCKFKVKKVAIDPSLLGEDKDMLEDLVMAAMNDAVAKAEATTQAKMAGFTAGLNLPP
                     GMGLPF"
     gene            complement(790..2160)
                     /locus_tag="VI195_06765"
     CDS             complement(790..2160)
                     /locus_tag="VI195_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824125.1"
                     /translation="MRELLDTAQYMPVAGRFKVYIIDEVHMLSRHAFNSMLKTLEEPP
                     EHVKFILATTDPQRLPVTVLSRCLQFNLKPLTPPLIAGHLRHVLEAEGIPFEEAALAQ
                     LGRAAAGSVRDSLSLLDQAIAYGGGRVEAQPVAEMLGSLGGDLLWPLLEQVVAGDGKR
                     LIAEADRIAERSVSFDAALEELAAILHRIALAQAGAPPDDDEPDAARVIEMASRLDAG
                     RIQVMYQVALLGRRDLPLAPDEYAGFTMALLRMLSFGQGAPAQRTPASSDGATTRTPA
                     EVAAREGASAASATRASRPSAPAASAPRAATPSAQSADAQAASPAVEFNGDWHALVQR
                     LNGLARMVAAHAELASYSNGHFEIVLPEAQRMYADKSYQDKLRADLATHFGSGVRLSV
                     RVGSTSGNSVAAARSREMAQKQASAAEAIEEDPFVRDLVRDLGAEVVSSTIRPAEDGN
                     QTNEQR"
BASE COUNT          309 a          775 c          763 g          347 t
ORIGIN      
        1 ccctcgaccg taaagttggt ggccaggatg acctccttca ccgcgccgtc cgtggcgcgc
       61 cgggccaggc ggtcgaggtg gatctcgcgg gggccgatgc catccagggg cgaaagccgc
      121 ccgagcagca cgaaatagag gccgggatag ctctgcgtct gctccatcat caggaggtcg
      181 ccgggcgtct ccacgacgca cagcaggctc gcgtcacgcc gcggcgaggc gcagagcgcg
      241 cacacctcgc tctccgtgaa cgtgttgcag cgcgcgcagt ggcggatgcg ctccagcgcc
      301 ccggaaagcg ccgcgcccag ccgcccggcg cccttctggt cccgctgcag caggtggtag
      361 gccatgcgct gcgcggattt gggcccgacg ccgggcaggc agcgcagcgc ctggatgagc
      421 tcctcgaggg ccgccggctt gtcgagcggt gcggccacct agaacggcag gcccatgccc
      481 ggcggcaggt tgaggcccgc ggtgaacccc gccatcttcg cctgcgtcgt ggcctcggcc
      541 ttggcgacgg catcgttcat cgccgccatc accaggtcct cgagcatgtc cttgtcctcg
      601 ccgaggaggc tcggatcgat cgcgaccttc ttcaccttga acttgcagga catggccacc
      661 ttgacgaggc ccgcgcccga ctggccctcg acgtcgatgt tggccagctc ctcctgcgcc
      721 ttgcgcatgt tctcctgcat ctgctgcgcc tgcttcatca gcgcgccgag tccgcccttc
      781 atcatgggtt cacctctgct cgttggtctg gttgccgtct tccgcgggac ggatggtcga
      841 cgagaccacc tcggcgccga gatcgcgcac gaggtcccgc acgaatggat cttcctcgat
      901 ggcctcggcg gcgctcgcct gcttctgcgc catctcgcgc gagcgtgcgg cggccacact
      961 gttgcccgag gtgctgccga cgcgaaccga aaggcgcacg ccggaaccga agtgcgtcgc
     1021 gagatcggcg cgcagcttgt cctggtagct cttgtccgca tacatgcgct gcgcttcggg
     1081 cagcacgatc tcgaaatgcc cgttgctgta cgacgcgagc tcggcgtgcg ccgccaccat
     1141 gcgcgcgagg ccgttcagcc gctgcaccag cgcgtgccag tcgccgttga attcgaccgc
     1201 gggcgacgcg gcctgcgcgt ccgccgattg cgcggatggt gttgcggccc ggggcgcgga
     1261 cgccgccggc gccgaggggc gtgatgcgcg cgtagccgac gccgccgatg cgccctcacg
     1321 cgcagcgact tcggccggcg tgcgagtggt cgcgccatcg gagcttgccg gcgtgcgctg
     1381 cgcaggcgcg ccctgcccga acgacagcat gcggagcagc gccatggtga agccggcgta
     1441 ctcgtcgggc gcaagcggca ggtcgcgcct gcccagcagg gccacctggt acatgacctg
     1501 gatgcggccc gcgtcgaggc gcgaggccat ctcgatcaca cgcgccgcat cgggctcgtc
     1561 gtcatcgggc ggcgcgcccg cctgcgccag cgcgatccga tgcaggatcg cggccagctc
     1621 ctccagcgcg gcgtcgaagg acacgctgcg ctcggcaatg cgatcggcct ccgcgatgag
     1681 gcgcttgccg tcgccggcca cgacctgctc cagcagcggc cagaggagat ctccgcccag
     1741 ggaaccgagc atctcggcaa ccggctgggc ttcgaccctg ccgccgccgt aggcgatggc
     1801 ctggtcgagc aggctcaagc tatcgcggac gctgccggcc gcggcgcggc ccaactgcgc
     1861 gagcgccgct tcctcgaacg ggatgccttc ggcttcgagc acgtggcgca ggtgcccggc
     1921 aatgagcggc ggggtgagcg gcttcaggtt gaactgcagg cagcgcgaga gcacggttac
     1981 cgggagacgc tgcggatccg tggtagcgag gatgaacttc acgtgctcgg gcggctcctc
     2041 cagcgtcttc agcatggagt tgaacgcgtg ccgcgagagc atgtgcacct cgtcgatgat
     2101 gtagaccttg aagcggccgg ccacgggcat gtattgcgcg gtatcgagca gctcgcgcat
     2161 ctcgtccacg cgcgtgttgg tggcggcatc cacc
//
LOCUS       DATDMP010000390         2477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2063215, whole genome shotgun sequence.
ACCESSION   DATDMP010000390 DATDMP010000000
VERSION     DATDMP010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063215"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..884
                     /gene="map"
                     /locus_tag="VI195_06770"
     CDS             63..884
                     /gene="map"
                     /locus_tag="VI195_06770"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY6824126.1"
                     /translation="MHVTIKTPEEQQKMRVAGKLAAEVLDMIGEHVVPGVTTEELDRV
                     CHDYIVNVQRSVPANLNYRGFPKTICTSVNHVVCHGIPNDKRLKAGDIVNIDVTVIRD
                     GFHGDTSRMYFVGKPPTHAERLAETCFAAMWRGIDTVRPGAHLGDIGHAIQSFVEQND
                     YSVVREYCGHGIGRVYHEDPQVLHYGEPGTGLELRPGMTFTIEPMVNAGKRHVRLLPD
                     GWTVITKDHSLSAQWEHTVLVTDSGVDVLTLGANGGRPARGVQSARAYQPADRQH"
     gene            905..>2477
                     /gene="glnD"
                     /locus_tag="VI195_06775"
     CDS             905..>2477
                     /gene="glnD"
                     /locus_tag="VI195_06775"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY6824127.1"
                     /translation="MGAADTELLEPTLAPLSPAPPWPLLLGLPARLAADASPRAWREL
                     LREAHAELKQRFLAEEPVEELVHARAALIDAVLRAAWQMHCAAQLDWALVAVGGYGRA
                     ELHPASDIDILLLVPQPPDAGGSAALERLVTLLWDIGLEVGHSVRTINECREECIGDV
                     SVMTTLLEARLLAGDGALFAAMRGALAPEHIWPVKQFFEAKVREQSERHLKANDTAYN
                     LEPNVKTGPGGLRDIQTIAWVAKRHFGTATLESLVTHGFVTPAELRRLRLARAFLWRV
                     RFGLHALTGRREDRLLFDHQIRLALAFGYEDASYTLAVEQFMQRYYRTVMDVSLLNEL
                     LLQLFREAILTESEPPRPLNARFQVRNGSLEAVSEEVFARTPSALLELFTVLQQNPEI
                     RGVRASTMRAVARSLWLIDEEFRQNPRHHRLFLEILRSPIGVTHQLRRMNTYGVLGRY
                     IPAFGRIVGRMQYDLFHAYTVDAHTIFVVSNLRRFAIPRYDQELPEASRVMQQLPRCE
                     IAYLAALFHDIAKVRG"
BASE COUNT          396 a          859 c          821 g          401 t
ORIGIN      
        1 atgtcttacg cgctcggcgc tggctcttgg cgccgctagc caccggcccc atttcggagc
       61 ttatgcacgt caccatcaag accccggaag agcagcagaa aatgcgggtt gccgggaagc
      121 tcgccgccga agtgctcgac atgatcggcg agcacgtggt gccgggagtg acgaccgagg
      181 agctcgaccg cgtctgtcac gactacatcg tgaatgtgca gcgctcggtg ccggccaacc
      241 tgaactatcg tggctttccg aaaaccatct gcacctccgt caaccacgtg gtgtgccacg
      301 gcatcccgaa cgacaagcgt ctgaaggccg gggacatcgt caatatcgac gtgaccgtga
      361 tccgtgacgg ctttcatggc gacaccagcc gcatgtactt cgtcggcaag ccgccgacgc
      421 acgccgagcg gctggccgag acctgcttcg cagcgatgtg gcgcggcatc gacacggtgc
      481 gcccgggcgc ccacctgggc gacatcggtc atgccatcca gagcttcgtc gagcagaacg
      541 actactccgt ggtgcgcgag tactgcggcc acggtatcgg ccgcgtgtac cacgaggatc
      601 cgcaggtgct gcattacggt gagccgggta ccggcctcga gctgcgcccg ggcatgacat
      661 tcaccatcga gccgatggtg aacgccggca agcggcacgt acgcctgctg cccgatggtt
      721 ggaccgtcat caccaaggac cactcgctct cggcgcagtg ggagcacacc gtgctcgtca
      781 ccgacagcgg tgtcgatgtg ctgacacttg gtgcgaatgg cggccggcct gcgcgcggtg
      841 tgcagtccgc gcgcgcgtat cagcccgccg atcgacagca ctgacggacg cgacacacgc
      901 acccatggga gctgcggaca cagagctgct cgaaccaaca ctcgcgccgc tctcccctgc
      961 tcctccctgg ccgctgctgc tggggctgcc ggcacggctc gcggccgatg ccagcccgcg
     1021 cgcctggcgg gagctgctgc gcgaagctca cgcggaactc aagcagcgct tcctcgccga
     1081 ggaaccggtc gaggagctgg tacacgcccg cgccgcactt atcgatgcgg tgctgcgtgc
     1141 ggcgtggcag atgcactgtg cggcgcagct cgactgggcg ctcgttgccg tcggcggcta
     1201 cggccgcgcc gagctgcacc cggcctccga catcgacatc ctgctgctcg tgccgcagcc
     1261 tccggatgct ggcggcagcg ccgcgctcga gcggctggtg accttgctgt gggatatcgg
     1321 tctcgaggtg ggtcacagcg tgcgcaccat caacgagtgc cgcgaggaat gcatcggcga
     1381 tgtgagcgtc atgacgacgc tgctggaagc gcgcctgctc gccggcgacg gggcgttgtt
     1441 tgcggcgatg cgcggcgcgc tggccccgga gcacatctgg ccggtgaagc agttcttcga
     1501 ggccaaggtg cgcgagcaga gcgagcggca tctcaaggcg aacgacaccg cctacaacct
     1561 cgagcctaac gtgaagaccg gtcccggcgg cctgcgcgac atccagacca tcgcctgggt
     1621 ggcgaagcgc cacttcggca ccgccacgct cgagtccctg gtgactcacg gcttcgtcac
     1681 gccggcggag ctgcggcggc tgcgcctggc gcgcgccttc ctctggcggg tgcgcttcgg
     1741 cctgcacgcg ctcaccgggc ggcgcgagga ccggctgctg ttcgatcacc agatccgcct
     1801 ggcgctcgcc ttcggctacg aggatgcctc ctacaccctc gccgtcgagc agttcatgca
     1861 gcgctattac cgcacggtga tggacgtgag cctgctgaac gagctgctgc tgcagctgtt
     1921 ccgcgaggcc atcctcaccg agagcgagcc gccgcggccg ctcaatgcgc gcttccaggt
     1981 ccgcaacggc tcgctcgagg cggtgagcga ggaagtgttc gcgcgcaccc cgtcggcgct
     2041 gctcgagctg ttcacggtgc tgcagcagaa cccggaaatc cgcggcgtgc gcgcctcgac
     2101 catgcgcgcg gtggccagga gcctgtggct gatcgacgag gagttccgcc agaatccgcg
     2161 ccaccaccgg ctgttcctcg agatcctgcg ctcgcccatc ggcgtgaccc accagctgcg
     2221 gcgcatgaac acctacgggg tgctggggcg ctacataccg gcgttcgggc gcatcgtcgg
     2281 gcgcatgcag tacgacctct tccatgccta cacggtcgac gctcatacga tcttcgtggt
     2341 gagcaacctg cggcgcttcg cgattccgcg ctacgatcag gagctgcccg aggcctcgcg
     2401 cgtcatgcag cagctgccgc gctgcgagat cgcctacctc gcggcgctgt tccacgacat
     2461 cgccaaggtg cgcggcg
//
LOCUS       DATDMP010000391         2591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1952048, whole genome shotgun sequence.
ACCESSION   DATDMP010000391 DATDMP010000000
VERSION     DATDMP010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952048"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VI195_06780"
     CDS             <1..505
                     /locus_tag="VI195_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6824128.1"
                     /translation="VQRARGGDRRAFERLYREHVGRVYGLCLRMTRDVQLAEDCTQDT
                     FINAWRALGKFETRSSLSTWLHRIAVNVSLAKRRKSGLVEPLPEEEEGSAAEWTLETP
                     VEVQELESAIGALPEGARDALVLHALYGYSHGEAAQMLGIAEGTCKAQLHRARRLLRE
                     RLGMEVN"
     gene            507..1106
                     /locus_tag="VI195_06785"
     CDS             507..1106
                     /locus_tag="VI195_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824129.1"
                     /translation="MAGPKDTTGRTVTSLRELPQSIEPAHDLWSGIESQLTALPVPAA
                     AAENAESAYRRALRRRWLAAAAMVACVAVGVWIGRSLLPGAAVGGAPPLPASRSTVPS
                     LAPTAFDAAYVSDPRYERQRRALVRALQERLAALPPPARAKVSASLAAIEKAKSDLEQ
                     ALGQDPGNALLQELLVNTYQDEMRVLTDVHEASDSGRGI"
     gene            1109..2008
                     /locus_tag="VI195_06790"
     CDS             1109..2008
                     /locus_tag="VI195_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY6824130.1"
                     /translation="MRIALRLTLALLPLLAGTTATASSDKTFDRQVPAQPRGVVEISN
                     VSGSIEVSGWDRNEVSVHAELDAGVERVDVSSDGGRTTIKVMLPHASSHGGEARLQVK
                     IPKASELNVSAVNADMISNGVLGVQRLSAVSGNVTAELGGADVELKTVSGDLRLKGHG
                     LPARAHLSTVSGSVQLEHGAGDLEASTVNGALVLSLDGARSVRARTTAGDVRLEGKLA
                     RGATLEASTVSGDVNVRASAEGGYAYEVSSFSGDITDCFNASPERVSKYAPGSRLEGT
                     RGEGAGHVRLKTMSGDVQLCDRP"
     gene            2043..>2591
                     /locus_tag="VI195_06795"
     CDS             2043..>2591
                     /locus_tag="VI195_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824131.1"
                     /translation="MSPSPSARHSPIATALLDWHARAGRHDLPWQHPRTPYRVWVSEV
                     MLQQTQVATVIPYYQRFLERFPDVRVLAAAPVDEVLHLWSGLGYYARARNLHRAAVRV
                     SAEHGGELPESFDALAALPGIGRSTAGAILALSGGVRLPILDGNARRVLARYFAVAGE
                     RGDGETEKRLWALAERCTPQARI"
BASE COUNT          410 a          837 c          940 g          404 t
ORIGIN      
        1 cgttcagcgg gcgcgtgggg gtgaccggcg ggctttcgaa aggctttacc gcgagcacgt
       61 cgggcgcgtt tacggcctgt gcctgcgcat gacgcgcgat gtgcagctgg cggaggattg
      121 tacgcaagac acgttcatca atgcctggcg cgcgctcgga aagttcgaga cgcgcagctc
      181 gctgtcgacc tggctgcatc gtattgccgt caatgtgagt ctggccaaac ggcgcaagag
      241 cggactggtc gagccgctac ctgaggagga ggaaggcagc gcggcggagt ggacgctcga
      301 gacgccggtg gaggtgcagg agctcgagag cgccatcggc gcattgcccg aaggggcgcg
      361 tgatgcgctg gtactgcatg ccctctacgg ctactcacat ggtgaggcgg cacagatgct
      421 cggcatcgcc gagggcacct gcaaggcgca gttgcaccgg gcgcgccggc tgctgcgcga
      481 gcggctcggt atggaggtga attgacatgg ccggtccgaa ggacacgact ggacgcacag
      541 tcacgtcgct gcgcgagctg ccgcagtcca tcgagcccgc gcacgacctg tggtccggga
      601 tcgaatcgca gctcaccgct ttgccggtac ccgccgcagc ggcggagaac gcggagtctg
      661 cataccggcg cgcactgcgg cggcgctggc tcgcggcggc cgccatggtg gcatgcgtcg
      721 ccgtgggcgt ctggattggc cgcagcctgc tgcccggcgc cgccgtgggt ggcgcacccc
      781 cgctccccgc cagccgatcc accgtgccga gccttgcgcc caccgctttc gatgccgctt
      841 atgtgagcga tccgcgctac gagcgtcagc gccgcgcgct ggtgcgcgcg ctgcaggagc
      901 ggctcgcggc gctgccgcct ccggcacgcg ccaaggtgag cgcgagcctc gccgccatcg
      961 agaaggcgaa gagcgacctg gagcaggcac tcggccagga tccgggcaac gcgctgctgc
     1021 aggagctgct cgtcaacact taccaggacg aaatgcgcgt cctgaccgac gtgcacgaag
     1081 ccagtgattc tggcaggggg atctgaagat gcgcatcgct ttgcgcctca cgctcgcgtt
     1141 gctgccgctg ctcgcgggca ctacggccac cgcctcgagc gacaagactt tcgatcggca
     1201 ggtgccggcg caaccgcgtg gcgtggtgga aatctccaac gtcagcggca gcatcgaggt
     1261 gagcggttgg gaccgcaacg aggtcagcgt gcacgcggag ctcgacgctg gtgtcgagcg
     1321 ggtcgatgtg agcagcgatg gcggccgcac gaccatcaag gtgatgttgc cgcacgcttc
     1381 cagccacggg ggcgaagcgc gactgcaggt gaaaattccg aaggccagcg agctgaatgt
     1441 ctcggcggtc aacgcggata tgatcagcaa cggcgtactc ggggtgcagc gcctcagcgc
     1501 ggtgagcggt aacgtcaccg ccgagcttgg cggtgccgat gtcgagctca agacggtgag
     1561 cggggatttg cggctgaagg gccacggtct gcccgcgcga gcgcacctga gcaccgtgag
     1621 cgggagtgtg cagctcgagc acggcgcggg cgatctcgag gcgagcaccg tgaacggagc
     1681 tctggtcctc tcgctcgacg gtgcgcgttc ggtgcgcgct cgcacgaccg ctggcgatgt
     1741 gcgcttggag ggcaagctgg cccgcggcgc gacgctcgaa gcttcgacgg tgagcggcga
     1801 tgtgaatgtg cgcgccagcg ccgagggcgg ctacgcctac gaggtttcga gcttcagtgg
     1861 ggacatcacc gactgcttca acgcgagtcc cgagcgggtg agcaaatacg cccccgggag
     1921 ccggctcgag ggcacacgcg gtgagggcgc cggccacgtg cggctgaaga ccatgagcgg
     1981 cgatgtgcag ctgtgcgatc gcccctgaag gggggttacc cgcgcgaggt tagaatgagc
     2041 gcgtgagccc gagcccgtct gcgcgccatt cacccattgc gactgcgctg ctcgactggc
     2101 acgcccgcgc cggtcgccac gatctgccgt ggcagcaccc gcgcacgccc taccgcgtct
     2161 gggtctcgga agtcatgttg cagcagaccc aggtcgcgac cgtcattcct tactaccagc
     2221 ggttcctcga gcgatttccc gacgttcgcg tgctcgccgc cgcgccggtc gatgaagtgc
     2281 tgcatctatg gtccggtctg ggctactacg cgcgtgcccg caacctgcat cgtgcggccg
     2341 tgcgcgtgag cgctgagcac ggcggtgagc tgccggagag cttcgatgcg ctcgccgcgc
     2401 tccccggaat cggccgctcg accgcggggg cgatcctcgc tctgagcggc ggagtgcgct
     2461 tgccgatcct cgatggcaac gcacggcgcg tgctcgcacg ctactttgcc gtcgccggcg
     2521 agcgcggtga cggcgagacc gagaaacgat tgtgggcgct cgccgagcgc tgcactccgc
     2581 aggcgcgcat t
//
LOCUS       DATDMP010000392         3432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_64183, whole genome shotgun sequence.
ACCESSION   DATDMP010000392 DATDMP010000000
VERSION     DATDMP010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64183"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VI195_06800"
     CDS             <1..449
                     /locus_tag="VI195_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824132.1"
                     /translation="SVAHGILRAEAKAVSSEALQQSFRAAMSGYLRAELAGRNACEFA
                     ASLLHDLLQAFGEPQPDGNSNESLIPDSQRFDGSTSLHFNENGKHASGWEIQVLEGIL
                     DTTDLITYRRLHCLHAFPERGTISQNAVQQSVTGTFVEHLSRSLTL"
     gene            825..1088
                     /locus_tag="VI195_06805"
     CDS             825..1088
                     /locus_tag="VI195_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824133.1"
                     /translation="MPTESQLRDHELRVRIRRLIGQGVLPVMVPKQIAGGYGSGQLCV
                     GCDQPIASAQVVYEVTDERNGRRLSFHLGCHVVWQLECAAHLR"
     gene            complement(1162..1773)
                     /locus_tag="VI195_06810"
     CDS             complement(1162..1773)
                     /locus_tag="VI195_06810"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY6824134.1"
                     /translation="MFTGIVQDIGRVLAREARGGDVRLTIGCGRLELSSARVGDSLCV
                     QGCCLTAVELDRNSFAVDVSRETLALTTLGELEPGALVNLEPALRAGDPLGGHLLSGH
                     IDGVALITEAHSDARSLRLSIETNVELARYLAPKGSVALDGVSLTINTVQGASFGVNI
                     IPHTQSNTTLGRLTAGARVNLEVDQLARYVARLLEPLSGLPRG"
     gene            complement(1787..2947)
                     /gene="ribD"
                     /locus_tag="VI195_06815"
     CDS             complement(1787..2947)
                     /gene="ribD"
                     /locus_tag="VI195_06815"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY6824135.1"
                     /translation="MTTPPQRSGSGRFSDFDRFAMQRALSLAARGLETTDPNPRVGCV
                     VAQRGRVLAEAWHERAGDAHAELVALRAAGGEAAGATVYVPLEPCSHHGRTPPCVEAL
                     VAARVARVVMAVGDPNPLVDGKGAAALRAAGIGVESGLMEEEATELNAGFFRRMRTGR
                     PLLRVKLAMSLDGRTALASGESRWITGEAARADVQRWRARSSAVLTGIGTVLADDPRL
                     DVRLASETGAARRQPLRVVLDSQLRTPPSARLLTTPGEALIFTTLTSPDDPRALSLSA
                     RGARLESLPLDGERLALQSVLDRLGELELNEVLVEAGATLAGELLRQGLADELLLYVG
                     LRLLGPAARALVALPPLARLADAPAFALVEVQQVGDDLRLRLRPGSAPPGPR"
     gene            complement(3103..3432)
                     /gene="nrdR"
                     /locus_tag="VI195_06820"
                     /pseudo
     CDS             complement(3103..3432)
                     /gene="nrdR"
                     /locus_tag="VI195_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
BASE COUNT          586 a         1175 c         1121 g          550 t
ORIGIN      
        1 tgtcggttgc acacggcatt ctgcgtgcgg aagcgaaggc tgtaagcagt gaagcgcttc
       61 aacagtcgtt ccgtgcggcg atgtccgggt atctccgcgc agaactggcg ggccggaatg
      121 cgtgcgagtt tgccgcatcc ctgctgcacg atctgctcca ggctttcggt gagccccagc
      181 cagatggaaa ttccaatgaa tccctcattc ccgacagtca acgcttcgac ggttccacct
      241 ccctccactt caacgagaac ggaaagcacg ccagtggttg ggaaatacag gtacttgagg
      301 ggatcctgga caccacagat ctcatcacct acaggaggct gcactgtctg cacgctttcc
      361 cggagcgcgg gacaatcagc caaaatgctg tccagcaatc ggttacgggt acttttgtgg
      421 agcatctgag ccgttccttg actctctgaa cgatatgtcc gccagcaggc cgacagtagc
      481 caatggcggc agtaaggttc aagggaggcc tagacctttg gctcaaccag cacatgcgtg
      541 accaactatg gcgcacagca gcagcggaag atcccattgg cgagatcgac cgcctcgcgt
      601 cggcactcgc tacctacggc tgcactccct atctcgacga cggatcggca gcccgtcagg
      661 taatgcgcaa acacacgacc tcactctggc gcgccctggc ttgggaagta gccagtctgg
      721 tacgaccgaa ccccgccact gccacgtggt gatgaatcgc agtgacggcc tgcagtcgta
      781 actaacggtg tctcggttcg cctatagctt tagggcacct cgccatgccc actgaatcac
      841 agttgcggga ccacgaactg cgggtgcgca ttcgccggct gatagggcaa ggcgtgctgc
      901 cggtcatggt gcccaaacag attgccggcg gctacggatc cggacagctt tgcgtagggt
      961 gcgatcagcc gatcgcgagc gctcaagtcg tgtacgaggt cacggacgag cgcaatggcc
     1021 gccggttgag ctttcatttg ggctgtcacg tggtgtggca gctggagtgc gcagcacacc
     1081 tcaggtaagc ggtgtgccta cgattcaccg gctggcggta attgccgcac cggccgcttt
     1141 cgtcgtgcgc tgcaagtgat ctcaaccgcg cggcaagccg ctgagaggct ccagcagccg
     1201 agcgacgtac cgtgcgagct ggtcgacttc cagattcacg cgcgcgccgg ccgtcaaccg
     1261 ccccagagtc gtgttgctct gagtatgcgg gataatgttc acgccgaagc tcgcgccttg
     1321 caccgtgttg atggtgaggc ttaccccatc gagcgcgacc gagcccttgg gcgcgaggta
     1381 gcgcgcgagt tcaacattcg tctcgatgct gagccgcagt gagcgcgcat cgctgtgcgc
     1441 ctcggtgatg agcgcgaccc catcgatgtg gcccgagagc agatgcccgc cgagtgggtc
     1501 cccggcccgc agcgccggct cgaggttcac gagcgccccg ggctcgagct ccccgagcgt
     1561 ggtgagcgcg agcgtctcgc gcgagacatc gacggcgaag ctgttgcggt cgagctcgac
     1621 cgcggtgagg cagcaacctt gcacacacag gctgtcgccc acgcgagcgc tggacagctc
     1681 gaggcgcccg cagccgatgg tgagccgcac atcgccacca cgcgcctcgc gtgcgagcac
     1741 ccggccgata tcctgcacga ttccggtaaa caagcgttcc gctccctcag cgcggcccgg
     1801 gcggcgcact gcctggccgc agccgcagac gcaggtcatc tccgacctgt tgcacctcga
     1861 ccagggcgaa ggctggagca tcggcgaggc gtgccagcgg cggcagcgcg acgagcgcac
     1921 gcgcggcagg accaagcagc cgcagcccta cgtacagcag caactcgtcc gcgagaccct
     1981 gacgcagcag ctcgcccgcc agagtcgcgc cagcctccac cagcacctcg ttgagctcga
     2041 gctcaccgag ccggtcgaga accgactgca gcgccagccg ctcgccatcg agcggcagcg
     2101 actcgaggcg cgcgccacgc gcactgagac tcagcgcgcg cggatcgtcc ggggaggtga
     2161 gtgtggtgaa gatcagcgcc tctccgggcg tcgtcaacag ccgtgcgctt ggtggggtgc
     2221 gcagctgact gtcgagcacg acgcgcagcg gctggcgtcg ggccgcgccg gtctcgctcg
     2281 ccagccgtac atcgagacgc ggatcatcgg caagcaccgt gccgatgccg gtgagcaccg
     2341 cggagctgcg cgcgcgccag cgctgcacat cggcccgtgc cgcctcgccg gtgatccagc
     2401 ggctctcgcc gctggcgagc gcagtgcggc catcgagact catggcgagc ttcactcgca
     2461 gcaatggccg tccagtgcgc atgcggcgga aaaagccggc attcagctcg gtcgcctcct
     2521 cttccatgag accgctctcg acgccgatac cggcggcgcg cagcgcagcg gctcccttgc
     2581 cgtcgaccag cggattcgga tcgccgacgg ccatcaccac gcgcgccacg cgcgctgcaa
     2641 ccagcgcctc gacgcacggc ggggtgcgcc cgtgatgact gcagggctcg agcggcacat
     2701 acacggtcgc tccggcggcc tcaccgccgg ccgcgcgcag cgccacgagc tcggcgtggg
     2761 cgtcaccggc gcgctcgtgc caggcttcgg cgagtacccg gccgcgctga gcgaccacgc
     2821 agccgacgcg cggattggga tcggtggtct ccagtccgcg cgcagcgagc gagagcgcac
     2881 gctgcatggc gaagcggtcg aaatcagaga acctaccgct accggagcgc tgcggcggcg
     2941 tagtcatttc ttgtcgttcg gtcccggccc ggcgtcgttc gcggccgggt caggaggcag
     3001 cagcggcagc tggtcgcgac tcgcggtgcg cacgccggcg cgcttgtgcc gcgcgccgtg
     3061 cgtaccgtgc agcgggcccg gggcctgagc gcgcgcgttg cgtaaccgct cgatctcttc
     3121 gtggaaggcc tcgacgtcct cgaagtgccg atagacggag gcgaagcgca cgtacgcgac
     3181 ctcgtcgagg tgccgcagct cctccatgac gagctcgccg accgcgcgcg cggagacctc
     3241 gcggtcgccg aggctgcgca cgcggtgaac gatccggctc accgcctcat cgatctgctc
     3301 gcgtggcacc ggccgcttct ccagcgcctt ttccatgccc gccctgagct tgccctcatc
     3361 gaatgcctcg cgcccccgat cacccttgat caccagcggc atgacgagct cggccgtttc
     3421 gaaggtggtg aa
//
LOCUS       DATDMP010000393         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_397338, whole genome shotgun sequence.
ACCESSION   DATDMP010000393 DATDMP010000000
VERSION     DATDMP010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397338"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VI195_06825"
     CDS             complement(<1..359)
                     /locus_tag="VI195_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6824136.1"
                     /translation="MAQILVVDDEVGIRELLSEILADEGHQVLLAESAGDARRLRERH
                     RPDLVLLDIWMPDTDGISLLKEWAASGQLGMPVVMMSGHGTIDTAVEATRIGAFGFLE
                     KPIALQKLLTTVGQALRT"
     gene            complement(363..>2061)
                     /locus_tag="VI195_06830"
     CDS             complement(363..>2061)
                     /locus_tag="VI195_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824137.1"
                     /translation="ELQVKARAIAAELADRPASQQGGVLGRLRDQAGVQEAVVVNASG
                     RVLASSSEDVTRLVPELPTPQALRQARATRTYAAVDAAAGKPLALRVVVPVATLALAD
                     EPRYLQLRQTVPEQFGRSAEAVEAAYRDYRELALSRQGLKSIYIVTLTLTLSMALLVA
                     IALAVLLANRLSLPLANLAQATQAVARGDFSRQTPVISRDELGVLTESFNSMTRQLEE
                     ARRVVEANRMALESAKARLENILANLSAGVLVFDGALALSICNHGARAILGADLESFA
                     AEMREQFRAHGAQAWQQQTELKGTGKTLLLRGTALADDPGGGHVLVFDDITQLMHAQR
                     ATAWAEVARRLAHEIKNPLTPIQLSAERLEMKLAGKLGGDDADALARGTRTIVNQVAA
                     LKNMVDDFRDYARLPAPVFAALDLNALVQEVLALYEASNAPIVRKLAPGLPQVRADSA
                     QIRQVLHNLVQNAQDALEHRKHAAEQPVIEVCTAAAGERVRLTVADNGGGFPEEMMAR
                     VFEPYVTTKPRGTGLGLAIVKKIIDEHRGELTIENRAARGASVTILLPASESSAKAA"
BASE COUNT          282 a          751 c          711 g          317 t
ORIGIN      
        1 gtgcgcagcg cctggccgac tgtggtcagc agcttttgca gagcgatcgg tttttccagg
       61 aacccgaacg ccccgatgcg cgtcgcctcc accgcggtat cgatggtgcc gtgcccggac
      121 atcatcacca ccggcatgcc gagctggccg ctcgcggccc actccttgag cagagaaatg
      181 ccgtcggtat ccggcatcca gatgtcgagc agcaccaggt cgggccggtg gcgctcgcgc
      241 aggcgccgcg cgtcacccgc gctctcggcg agcagcacct ggtgaccctc gtcggcgagg
      301 atttccgaga gcagctcgcg gatgccgact tcgtcgtcaa cgaccaggat ctgcgccatg
      361 cgtcaggccg ccttcgccga ggactcggac gcgggcagca ggatcgtcac cgacgcgccc
      421 ctggcggcgc ggttctcgat cgtcagctcg ccgcggtgct cgtcgatgat cttcttcacg
      481 atcgccagtc ccagcccggt gccgcgcggc ttggtggtca catacggctc gaagacccgc
      541 gccatcatct cctccgggaa gccaccgcca ttgtcggcga ccgtgagccg cacgcgctcg
      601 ccggccgccg cagtgcagac ttcgatcacc ggctgctccg cggcatgctt gcggtgctcg
      661 agcgcgtcct gggcgttctg caccagattg tggagcacct gccggatctg cgccgaatcg
      721 gcgcgcacct ggggcaggcc tggcgcgagc ttcctgacga tgggtgcatt cgaggcctcg
      781 tagagcgcaa gcacttcctg taccagcgcg ttcaggtcga gcgccgcgaa caccggggcg
      841 ggcaggcgcg catagtcgcg gaaatcgtcc accatgttct tcagcgccgc gacctggttg
      901 acgatggtgc gggtgccacg cgcgagcgcg tcggcatcgt cgccgccgag cttgccggcg
      961 agcttcatct ccaggcgctc ggccgagagc tggatcggcg tgagcgggtt cttgatctcg
     1021 tgcgcgagcc gccgcgccac ctcggcccag gcggtggcgc gctgcgcatg catcagctgc
     1081 gtgatgtcgt cgaacaccag cacgtgaccg ccgccgggat cgtcggcgag cgcggtgccg
     1141 cgcagcagca gcgtcttgcc cgtgcccttg agctcggtct gctgctgcca ggcctgcgcg
     1201 ccgtgcgcac ggaactgctc gcgcatctcc gcggcaaatg actcgagatc cgcgccgagg
     1261 atcgcgcgcg cgccgtggtt gcagatcgac agcgccagcg cgccgtcgaa caccagcacg
     1321 ccggccgaga gattggcgag gatgttctcc agccgcgcct tggcgctctc cagcgccatg
     1381 cggttcgcct cgaccacgcg ccgcgcctct tccagctgcc gcgtcatcga gttgaaggat
     1441 tcggtgagca cgccgagctc gtcgcggctg atcaccggcg tctggcggct gaagtcgccg
     1501 cgcgccaccg cctgggtcgc ctgcgcgagg ttggcgagcg gcagcgacag gcggttcgcg
     1561 agcagcaccg cgagcgcgat cgccaccagc agcgccatcg acagcgtgag cgtgagcgtg
     1621 acgatgtaaa tgctcttcag gccctggcgc gagagcgcga gctcgcggta gtcgcggtag
     1681 gcggcctcga cggcctcggc gctgcgaccg aactgctccg gcacggtctg ccgcagctgc
     1741 aggtagcgcg gctcgtcggc gagcgcgagc gtcgccaccg gcaccaccac gcgcagcgcg
     1801 agcggcttgc cggccgccgc atcgacggcg gcgtaggtgc gggtggcgcg cgcctgccgc
     1861 agcgcctgcg gcgtcggcag ctccggcacc agccgcgtca cgtcctcgct cgagctcgcc
     1921 agcacgcgcc cgctcgcgtt gacgacaacc gcctcctgca cgcccgcctg gtcgcgcagg
     1981 cggccgagca cgccgccctg ctggctcgcc ggccggtcgg cgagctctgc agcgatggcg
     2041 cgcgccttca cctgcagctc g
//
LOCUS       DATDMP010000394         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1618978, whole genome shotgun sequence.
ACCESSION   DATDMP010000394 DATDMP010000000
VERSION     DATDMP010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618978"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VI195_06835"
     CDS             complement(<1..648)
                     /locus_tag="VI195_06835"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY6824138.1"
                     /translation="MLQLYQAQLGSRLMLGTARYPSPAVLADAVRAGRAEVVTVSLRR
                     ESAGERAGQAFWSLIRELGVRVLPNTAGCHSAKEAVTTAHMAREVFGTPWIKLEVIGE
                     DDTLQPDVFGLVDAACTLCAEGFEVFPYTTEDLVVAERLLAAGCRVLMPWGAPIGSGR
                     GLNNLFGLKALRAHFPDVPLIIDAGLGVPSHAAQALELGFDAVLINTAVAQARDPV"
     gene            complement(648..845)
                     /gene="thiS"
                     /locus_tag="VI195_06840"
     CDS             complement(648..845)
                     /gene="thiS"
                     /locus_tag="VI195_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY6824139.1"
                     /translation="MMILVNGAWRETRCAELARALEELGFGTSVVSTAVNGEFIGAGE
                     RARTLLSDGDRLEVLAPMQGG"
     gene            complement(814..1818)
                     /gene="thiO"
                     /locus_tag="VI195_06845"
     CDS             complement(814..1818)
                     /gene="thiO"
                     /locus_tag="VI195_06845"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEY6824140.1"
                     /translation="MHVTIIGAGIAGLSCALELSERGASVELCERAPQLAAAGCSWFA
                     GGMLAPWCERENSAPLIAELGAESLRWWRAHFAGTVVNGTLVVAHPRDTAELLQFARR
                     TAHCESIRAQAISALEPDLSGRFSHGLYFPEEGHLDPRAALSALAARLGERGVAIRFG
                     VDAAPRAGRVVLDCSGLAARSRLVDLRGVKGEMLLVRLPELNLTRPVRLLHPRLPLYV
                     VPRESGLYMVGATMIESDDATRISARSMLELLSAAYALHPAFGEAQIVEIGTGVRAAF
                     PDNLPRLRRAGGALHVNGLYRHGFLLAPALARRAADVLLDGRHYPEVMDDDPGERRLA
                     "
     gene            complement(1824..>2956)
                     /gene="thiC"
                     /locus_tag="VI195_06850"
     CDS             complement(1824..>2956)
                     /gene="thiC"
                     /locus_tag="VI195_06850"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY6824141.1"
                     /translation="ATLWGADTVMDLSTGKKIHETREWIVRNSAVPIGTVPIYQALEK
                     VGGVAEELTWEIYRDTLIEQAEQGVDYFTIHAGVRLPYVPLTVNRVTGIVSRGGSIMA
                     KWCLAHHRDSFLYENFAEICEICRAYDVSFSLGDGLRPGCIADANDAAQFAELETLGE
                     LTRIAWAHDCQVMIEGPGHVPMHKIKENMDKQLASCGEAPFYTLGPLTTDIAPGYDHI
                     TSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRNDVKTGVITYKIAAHAADLAKGHPAAR
                     ARDDALSRARFDFRWQDQFNLSLDPDTACAFHDATLPKEGHKLAHFCSMCGPKFCSMR
                     ISHEVRAEARSAGMAEMSARFRAGGGELYVPLPQRAAHGRES"
BASE COUNT          501 a         1033 c          984 g          438 t
ORIGIN      
        1 cactggatcg cgcgcctgtg ctacggcggt attgatgagc acggcatcga agccgagctc
       61 gagtgcctgc gccgcgtgcg aggggacgcc gagtccggca tcgatgatga gcggcacgtc
      121 cgggaagtgt gcccgcagcg ccttcagtcc gaacagattg ttgagtccac ggccggaacc
      181 gatgggcgca ccccagggca tgagcacccg gcagcccgcg gcgagcagac gctccgcgac
      241 caccaggtcc tccgtggtgt aggggaatac ctcgaaccct tccgcacaca gcgtgcatgc
      301 cgcatccacc agcccgaaca catccggctg cagcgtatcg tcctcgccga tcacttccag
      361 cttgatccag ggcgtgccga acacctcgcg cgccatgtgt gcggtagtga ccgcttcctt
      421 cgccgaatga cagccggccg tgttgggcag cacccgcacc ccgagctcac ggatgagcga
      481 ccagaacgcc tgcccggcgc gctcgccggc agattcgcgg cgcagcgaga ccgtgacaac
      541 ctcggcgcgc ccggcgcgca ccgcatccgc cagcaccgca ggtgagggat agcgcgcggt
      601 gcccagcatg agccgcgagc cgagctgcgc ctgatagagc tgcagcatca accgccctgc
      661 atcggcgcga gcacctcgag ccggtcgcca tcgctcaaca gggtgcgtgc acgctcaccc
      721 gcgccgatga attcgccatt caccgcggta ctcaccacac ttgtgccgaa gccgagctcc
      781 tccagggccc gggccagctc ggcgcagcgt gtctcacgcc aggcgccgtt caccaggatc
      841 atcatccatc acctccgggt agtggcggcc gtcgagcagc acatccgcag cacgacgcgc
      901 cagcgccggc gccagcagga agccgtgccg gtacaagccg ttgacgtgca gcgcgccacc
      961 ggcgcggcgc agccgcggca ggttgtcggg gaatgcagcg cgcacaccgg tgccgatctc
     1021 gacgatctgc gcttcgccga aggccgggtg cagcgcatac gccgccgaca agagctccag
     1081 catggagcgc gcgctgatgc gcgtcgcgtc atcgctctcg atcatggtcg cgcccaccat
     1141 gtagagcccc gactcgcgcg gcaccacgta tagcggcagc cgcggatgca gcaagcgcac
     1201 cggccgggtg agattcagct cgggcagccg cacgagcagc atctcgccct tcacaccgcg
     1261 caggtccacc aaccgcgagc gcgcggcgag cccactgcag tcgagcacca cgcggccggc
     1321 gcgcggcgcg gcatcgacac cgaagcgaat ggcgacgccg cgctcgccga ggcgcgcggc
     1381 gagcgcgctg agcgcggcgc gcggatcgag atgcccctcc tcggggaagt agagcccgtg
     1441 gctgaagcgg ccgctcaaat cgggctcgag tgcgctgatg gcttgcgcac ggatgctttc
     1501 acagtgggcg gtacggcggg cgaactgcag cagctcagcg gtgtcacgcg ggtgtgccac
     1561 gacgagcgtc ccgttcacga ccgtgccggc aaagtgcgcg cgccaccagc gcagactttc
     1621 cgcgccgagc tccgcgatga gcggcgcgga attctcacgc tcgcaccagg gtgccagcat
     1681 ccccccggcg aaccaggagc agcctgcggc ggcaagctgt ggagcgcgtt cgcagagctc
     1741 gacgctcgcc ccgcgctcgc tcagctcgag cgcgcagctg agtccagcaa tgccggcacc
     1801 gatgatggtg acgtgcatgc ccttcagctc tcgcggccgt gcgccgcgcg ctgtggcagc
     1861 ggcacgtaca gctcgccgcc cccggcgcgg aaacgcgcgg acatctccgc catgcccgca
     1921 ctgcgggcct cggcgcgcac ctcgtgcgag atgcgcatcg agcagaattt tggcccgcac
     1981 atcgagcaga aatgcgccag cttgtgccct tccttgggca gcgtcgcatc atggaaggca
     2041 caggcggtat cggggtcgag cgacagattg aactggtcct gccagcggaa atcgaagcgc
     2101 gcacgcgaga gcgcatcgtc gcgggcgcgc gccgccgggt gtcccttggc gagatccgcg
     2161 gcgtgcgccg cgatcttgta ggtgatgacg ccggtcttca cgtcgttgcg gtccggcagc
     2221 ccgaggtgct ccttgggcgt gacatagcag agcatagcgg tgccgaacca gccgatcatg
     2281 gcggcgccga tcgccgaggt gatgtgatcg tagccgggcg caatgtcggt agtgagcgga
     2341 ccgagggtat agaacggcgc ctccccgcag ctcgcgagct gcttgtccat gttctccttg
     2401 atcttgtgca tgggaacgtg gccaggcccc tcgatcatga cctgacagtc gtgcgcccag
     2461 gcgatgcggg tgagctcccc gagggtctcg agctcggcaa actgggccgc gtcgttggca
     2521 tcggcgatgc agccggggcg cagcccatcg ccgagcgaga agctcacgtc gtaggcgcgg
     2581 cagatctcgc agatctcggc gaaattctca tacaggaagc tgtcgcgatg atgtgccagg
     2641 caccatttcg ccatgatcga gccgccgcgc gagacgatgc cggtgacgcg gtttacggtg
     2701 agcggcacgt acggcagccg cacgccggcg tgaatggtga aatagtcgac gccctgctcg
     2761 gcctgctcga tgagggtgtc gcggtagatc tcccaggtga gctcctccgc cactccaccc
     2821 accttctcga gcgcctgata gatcggtacc gtgccgatcg gcaccgccga attccgtact
     2881 atccactcac gcgtttcgtg gatcttcttt ccggtcgaga gatccatcac cgtgtcggcg
     2941 ccccacagcg tggccc
//
LOCUS       DATDMP010000395         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1841408, whole genome shotgun sequence.
ACCESSION   DATDMP010000395 DATDMP010000000
VERSION     DATDMP010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841408"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..285
                     /locus_tag="VI195_06855"
     CDS             139..285
                     /locus_tag="VI195_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824142.1"
                     /translation="MHVLLRASDRNLIRVSVVLGQTAALVARDILRVRRKPARLLERV
                     TWQW"
     gene            complement(269..601)
                     /locus_tag="VI195_06860"
     CDS             complement(269..601)
                     /locus_tag="VI195_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824143.1"
                     /translation="MSDARTVEAITHAIEAAAKSNLALVDELTQSRVIQQRMANTLIE
                     IHSGQQQLIEQMGKLVERQNDHGKRQSDSESRIRVLESKHDRHVEQTEKRLERLERAA
                     PKHLTTAK"
     gene            complement(598..1233)
                     /locus_tag="VI195_06865"
     CDS             complement(598..1233)
                     /locus_tag="VI195_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824144.1"
                     /translation="MTAELYLWADAAVALCEYACGWDKGRGKDDPVYLEVVEHRDVPP
                     MRAKYSSCGDLAWWLLYRLGLRKPWMNRAANHTYNIGMNVADLSGCPAHIVPGADFVP
                     EPGDILEIWNRPGGEDAHVCVVVAVPFFEKITLANYGAGGMSPSAWPGANIHDADWRH
                     SVAGHYVGTRRLQRVVRLASIVPLLTEQVDLTGARVTGELIDALGAKWSEK"
     gene            complement(1230..1415)
                     /locus_tag="VI195_06870"
     CDS             complement(1230..1415)
                     /locus_tag="VI195_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824145.1"
                     /translation="MTAASLLAAVSAIVETVREAPQLVSAVEEVFASLRAKEDPTPAM
                     RRLEAAAAAHELRIPWP"
     gene            complement(1412..1639)
                     /locus_tag="VI195_06875"
     CDS             complement(1412..1639)
                     /locus_tag="VI195_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824146.1"
                     /translation="MRPLGYAALACLLGANALCLADCGGAQPKAQAQLAAIVAGCKVS
                     LDLERDAGEAGAIGETEQGCRAALHTWERAK"
     gene            complement(1636..1863)
                     /locus_tag="VI195_06880"
     CDS             complement(1636..1863)
                     /locus_tag="VI195_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824147.1"
                     /translation="MTPQTKQLLSALGVAILGAIITTLVPYVAHLPVAWQGIVNAALV
                     GVAHYLPTLGTKDVTRAEVTKQVNEIVQAEK"
     gene            complement(1876..2196)
                     /locus_tag="VI195_06885"
     CDS             complement(1876..2196)
                     /locus_tag="VI195_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824148.1"
                     /translation="MPKTIQTGAVQADADLCNAIDPPTQGCAAGGGIHVAIPGDWLTR
                     IQAAQPVPGCTYHALLDDGHGVLSLVVSDAAIRSLGIPAIVNALPGPLKAQAALLNAK
                     LASA"
BASE COUNT          452 a          858 c          835 g          495 t
ORIGIN      
        1 tctcgaagcc aaagccgtga cgccagcgac caccagcccc acgccgacca acgcccactt
       61 cggcagtccg cgcagcttca ctgggccgag ctcgactgta ggctgcgaat cggcatcagg
      121 ctgcgagtgc atatggacat gcacgtgctg ctgcgcgcca gtgaccgtaa tctcatccgt
      181 gtcagcgtcg tactcgggca gacggcggcg ctcgtcgcgc gcgatatcct tcgcgtccga
      241 cgcaaaccgg cgcgcctgct cgagcgggtc acttggcagt ggtgagatgc ttcggcgcgg
      301 cacgctcaag ccgctcaagg cgtttttcgg tctgctcgac atggcgatcg tgtttcgact
      361 ctagcacgcg gatacggctc tcggagtcgc tctggcgctt gccgtgatca ttctggcgct
      421 cgaccagctt gcccatctgc tcgattagct gctgctgccc tgagtgaatc tcgatgagcg
      481 tgttcgccat gcgctgctgg atgactcggc tctgagtgag ctcgtcgacc agcgcgagat
      541 tcgatttggc tgccgcctcg atggcgtggg tgatggcctc tacagtccgc gcgtcgctca
      601 tttttcgctc catttcgcgc caagcgcgtc tatcagctcc cccgtcactc gggcgccggt
      661 gaggtcgacc tgctcggtca gcagcggaac aatgctcgcg aggcgcacga cgcgctgcaa
      721 tcgacgcgtg ccgacgtagt ggcctgccac ggagtggcgc caatccgcat cgtggatgtt
      781 ggccccaggc caagcactcg ggctcatgcc gccagcgccg tagtttgcca gcgtgatttt
      841 ctcgaagaac ggcacggcaa ccacgacgca cacgtgcgcg tcctcaccgc caggcctgtt
      901 ccaaatctcg agaatgtcgc cgggctcggg aacgaagtca gcccccggca cgatgtgcgc
      961 agggcacccg ctcaggtcgg ccacgttcat gccaatgttg taggtgtggt tcgcggcgcg
     1021 gttcatccac ggcttgcgca gtccgagccg gtagagcagc caccaggcga ggtcgccgca
     1081 gctcgagtac ttcgcgcgca tgggcggcac gtcccggtgc tcgacgacct ccaggtacac
     1141 agggtcgtcc ttgccgcggc ctttgtccca gccgcacgcg tactcgcaca gcgcgaccgc
     1201 cgcgtccgcc cacaggtaga gctcggcggt cacggccagg gaatccgaag ctcgtgcgcg
     1261 gcagccgcag cttcgaggcg ccgcatggcc ggcgtcgggt cttccttggc tcgcagcgag
     1321 gcgaacacct cttccacggc gctcacgagc tgcggagcct cgcgcacggt ctcgacgatg
     1381 gcgctgacag cggcgagcag gctagcggcg gtcatttggc gcgctcccag gtgtgcaggg
     1441 ctgcccgaca tccttgctcg gtctcgccga ttgcaccggc ctcgccagcg tcgcgctcga
     1501 ggtcgagcga caccttgcag ccggccacga tcgccgcgag ctgcgcttgc gcctttggct
     1561 gcgcgccgcc gcaatcagcc aggcacaaag cattagctcc cagcagacag gccagcgccg
     1621 catagccgag cggcctcatt tctccgcctg cacaatctcg ttgacctgct tggtcacctc
     1681 agcgcgggtt acgtccttgg tgccgagcgt cggcaggtag tgggccacgc cgactagcgc
     1741 agcgttcacg atgccctgcc aggcaaccgg caggtgcgcg acgtatggga ccagcgtggt
     1801 gatgatggcg ccgaggattg caacgcctag tgcggagagt agctgcttgg tttgaggagt
     1861 catggtgttg ttctttcagg ccgaagccag tttcgcgttg agtaatgcgg cctgcgcttt
     1921 cagcgggccg gggagcgcgt tcacgatcgc ggggatgccc agcgatcgga tcgccgcgtc
     1981 cgagacgacc agcgaaagca cgccatgtcc gtcatcgagc aacgcgtggt aggtgcatcc
     2041 cgggacaggc tgagcagcct gaatgcgcgt gagccaatcg ccaggaatcg ctacatggat
     2101 accgcctcct gctgcgcacc cctgagtcgg cggatctatg gcgttgcaca gatcggcgtc
     2161 tgcttgcact gcaccggttt ggattgtctt cggcatcaca ccacgacgct ccctggccca
     2221 tatccaccta gtgaattgag cgccgagtcg actgctgcca gctgagcggc tgacataggg
     2281 gggcagatta cgaacgcaaa aaactggggc atcgtggctg ggaatgcccc tgcgagcgag
     2341 ccagccccga tcgacatgcc atttactaac gcattggagg cgctcgcccc cgtgacatcg
     2401 gcagccgcgc caaccttcag caggtcgctc gttgagccgg tatacgtggc tctaatacgc
     2461 gtaggcgtag ccgtcagtac ggtggaaaca ttgccgttta tgccgcaata ttgaattgtg
     2521 cgattcagag cacctgtgtc gctatatagc gtcgcggaac tgttggatcc gacccagatc
     2581 tgctggccac ccttcgcgag cggagacatc accaagtaga aagaaaatgg tgtggtagca
//
LOCUS       DATDMP010000396         3697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_731493, whole genome shotgun sequence.
ACCESSION   DATDMP010000396 DATDMP010000000
VERSION     DATDMP010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731493"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="betI"
                     /locus_tag="VI195_06890"
     CDS             complement(<1..903)
                     /gene="betI"
                     /locus_tag="VI195_06890"
                     /inference="COORDINATES: protein motif:HMM:NF001978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HEY6824149.1"
                     /translation="MPLNTQSTIRAPAPRTGITRDEGEDARRVQLIEVTIDSLAEVGY
                     VGTTLAEIARRAGVSPGLVAHYFGEKDGLLEAAFRTLARVVAMNARERLALARTPRAR
                     VQALIDANLGPENFDKRTGIAWLAFWGQVLHVEGLKRVQTAYQRRMLSNLRSDLRRMI
                     PGEAARSLAAMIAAMIDGVWLRAALSEWQEADSESAQALLTAFVDGRLRELSRPQGSD
                     GPDPAAGHAGGRNTPRAQESIRVINPATGEELAVLAVDGPQQVDAAVARARVAQKAWA
                     AQTAAQRGRVLQRAARLLRERNDEL"
     gene            1262..2806
                     /locus_tag="VI195_06895"
     CDS             1262..2806
                     /locus_tag="VI195_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6824150.1"
                     /translation="MVKNEAVARTDSGMEHFGYKESLARSIGAFGSFAAGVSYISILT
                     GTFQLFYFGFGTAGPAYLWSWPLVLLGQLAVALCFMELAAKYPIAGSVYNWSKLLGSP
                     IVGWSSGWLMLTASIVTLSAVVLALQLNLPRLWSVFQIIGDGAGTYDFATNAVILGTV
                     LIVFTTVVNALGVRLMATINSAGVFVELIAACLIALFLAVNIKRGPEVFFSTNGYGAG
                     KSWGFLGDFLVASLASGYVMYGFDTASSLGEETLEPRRTAPRAILRAILASFVIGGAI
                     LVFAIMAAPNLHDPRIGSSSGGLQYIVEQVMWGPLGKIFLVCIVVAVTVCSLAVHTAA
                     IRLTFAMARDNALPFGEWLARVNSKTRTPIVPAVVIGVIAELILVLNIGQPKIFTVLT
                     SIAVIMIYLAYLMVTGPLLKKRLQGQWPPADLAAGGYFSMGRWGMFVNIVAVLWGAGM
                     ALNLAWPREAVYGAPWYNTWGAFVYIAVILGAGLWWYAAKGCHHIGTLASHTLTPNAD
                     SLQAEL"
     gene            3047..3547
                     /locus_tag="VI195_06900"
     CDS             3047..3547
                     /locus_tag="VI195_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824151.1"
                     /translation="MAQSTPAGTSAAPAASSGSSGTSKQNLAEDIKLLRKDVRTEKAK
                     IMGAAMGLDADQAKKFWPIYKDYDKQLSKLNDVRLGNIIEYAKTYDSMTENKADELVN
                     GAIAYRKKRIDLLANTYDKVRGALGAPLAARFLQVEDTLLNLIDLQIQSNLPLMWGPE
                     KGASKP"
BASE COUNT          628 a         1195 c         1147 g          727 t
ORIGIN      
        1 gagctcgtcg ttgcgctcgc gcagtagccg cgccgcgcgc tgcagtaccc ggccgcgctg
       61 cgcggcggtc tgcgccgccc aggctttctg cgccacccgt gcgcgcgcca ccgcggcatc
      121 cacctgttga ggaccatcga cggcgagcac ggccagctcc tcgcccgtcg ccgggttgat
      181 caccctgatc gactcttgtg cgcgcggggt gtttcgtccg cccgcatggc ctgcggcggg
      241 gtccggcccg tcgctgccct gcgggcgact gagctcgcgt agccggccgt cgacgaatgc
      301 agtcagcagt gcctgcgcgc tctcgctgtc ggcctcctgc cactccgaca acgccgctcg
      361 cagccacacc ccatcgatca tggcggcgat catggccgcg aggctgcggg cagcctctcc
      421 cggtatcatc cgccgcagat cactgcgcag gttagacaac atgcgccgct ggtacgcggt
      481 ctgcactctt ttcagtccct cgacatgcag cacctggccc cagaaggcaa gccaggcgat
      541 cccggtacgc ttgtcgaaat tctccggtcc gaggttggca tcgatgagtg cctgcacgcg
      601 tgcacggggc gtgcgtgcga gcgcgagccg ctcgcgagcg ttcatcgcga ccacgcgcgc
      661 gagtgtgcga aatgccgctt cgagcagccc atccttttcg ccgaagtaat gcgcgacgag
      721 ccctggcgag acgccggccc tgcgggcgat ttccgcaagc gtggtgccga cgtacccaac
      781 ttcggcgaga ctgtcgatcg tgacctcgat cagctgcaca cggcgtgcgt cctcgccctc
      841 atcgcgcgtg atgcccgttc ggggtgccgg cgctcgaata gttgattgcg tattcaatgg
      901 catctccgga cccattctag gatacctgtc ttcaagcgct cctgaggcag ctgcgttatg
      961 acaattttta ttctattatc aaaaagttgc cagatactcc gagcaccgaa gatcgagccg
     1021 ttaaacgcgt attcaaagta tcaggcgaga tcatgaattg cgtcacttcg ggggtcaagg
     1081 ggacgggcca ccgccatcga gatgcttttt catgctcgcc tcagtgctgt gtagggtggc
     1141 gctcgccgat cccacgacag ccctttaagc actctgctgc cgccccttcg ctaccgcacc
     1201 cggatgaggg cgcaggcagt gctgctcacg agtgatgagc cactccagag aggaccaacg
     1261 cgtggttaag aacgaggctg tagcacgaac cgacagcggc atggagcact tcggctacaa
     1321 agaatcgctc gcccgcagca tcggggcgtt cggcagcttc gcggccggcg tcagctatat
     1381 ctcgatactc accggcacct ttcagctgtt ctacttcggc ttcggcactg ccggtccggc
     1441 ctatctgtgg tcctggccgc tggtgctgct cggtcagctg gcggtcgcgc tgtgcttcat
     1501 ggaattggcc gccaaatacc cgatcgccgg atcagtctat aactggtcca agctattggg
     1561 cagcccgatc gtgggttggt cttcgggctg gctgatgctg actgcctcca ttgtcaccct
     1621 gtctgcggta gtgctagcgc tgcaactcaa tctgccgcgg ctgtggagcg tcttccagat
     1681 catcggcgat ggcgcgggca cctacgattt cgccaccaac gcggtgatcc tcggcacggt
     1741 gctgatcgtg ttcaccacag tcgtcaatgc gctcggcgtg cggttgatgg cgacgatcaa
     1801 cagcgcgggc gtcttcgtcg agctgatcgc tgcgtgcctc atagcgctct tcctggccgt
     1861 caacatcaag cgcggtcccg aagtcttctt ttccaccaac ggctacggtg ccggcaaaag
     1921 ctggggcttt ctcggtgatt ttctcgtggc atcgctcgcc tccggctacg tgatgtacgg
     1981 cttcgacacc gccagctcgc ttggggaaga aaccctggag ccgcggcgca ccgctccgcg
     2041 ggcgattctg cgcgccatcc tcgcctcgtt cgtcatcggc ggtgccatcc tggtgtttgc
     2101 gatcatggcg gcgcccaatt tgcacgaccc gcgcatcggt agcagcagcg gcggcttgca
     2161 atacatcgtc gagcaggtga tgtggggtcc gctcggcaag atctttctcg tctgcatcgt
     2221 ggtcgcggtg acggtgtgct cgctggccgt gcacaccgcg gccatccgcc tgaccttcgc
     2281 gatggcacgc gacaatgcat tgcctttcgg agaatggctc gcgcgggtga attccaagac
     2341 gcgcacgccc atcgtgcctg ccgtcgtgat cggcgtgatt gccgagctga tcctggtgct
     2401 caacatcggt cagccgaaga ttttcacggt gctgacctcc attgcggtca tcatgatcta
     2461 cctggcgtac ctgatggtga cgggcccact gctgaagaag cgcctgcaag ggcaatggcc
     2521 gccggcagat ctcgcggccg ggggctactt cagcatgggc aggtggggaa tgttcgtgaa
     2581 catcgtcgcg gtgctgtggg gcgcggggat ggcgctcaac ctggcctggc cgcgtgaagc
     2641 cgtctatggc gcaccctggt acaacacctg gggtgccttc gtctacattg ccgtaattct
     2701 cggcgcgggt ctgtggtggt acgccgccaa aggctgtcat catatcggca cgctggcctc
     2761 gcacacgctg accccgaatg ccgactcact gcaggcggaa ctgtgacgcg gcgcggcgtg
     2821 cgcgcatcag gtgccgcccg caccttgcat tcgtgcgacc tcgggccgat ggcggccgcg
     2881 cgtgctttgg cgttagcatc gcgccgcgat tacacacagg gccttgctgc gcggtcgcag
     2941 cattcgttcc gtaacgggat ataaaaacgg aggatccata atgaagcgtc cattcactgg
     3001 agtcggactt gccacaatcg tccttttggt ctgcagctcc gcagccatgg ctcaatcgac
     3061 tccggcgggc acgtccgctg cgcccgcggc gagctccggc tcctcgggga cgtccaagca
     3121 aaacctggcc gaagacatca agctgctgcg caaggacgtg cgcaccgaga aggcgaagat
     3181 catgggcgct gccatggggc tcgatgccga ccaggcaaag aagttctggc cgatttacaa
     3241 ggattacgac aagcagctct ccaagctcaa tgacgtgcgc ctgggcaaca tcatcgaata
     3301 cgcaaaaacc tacgacagca tgaccgagaa caaggcggat gagctggtga acggcgcgat
     3361 cgcttaccgg aagaagcgca tcgacctgct ggccaacact tatgacaagg tgcggggcgc
     3421 cctcggtgca ccgctggccg ctcgcttcct gcaggtggag gacacgctgc tgaatctcat
     3481 tgacctgcag attcagtcga acctgccgct gatgtggggg ccggagaagg gcgccagcaa
     3541 gccctaacgg ggcaaacgct caccgacgcg ctccaccggg caaccgcgca ttgcctggtg
     3601 gggcgcgttc ggcgcgcccg gcccgctatt ccgtgatgat gcggtcggcc cactcgacca
     3661 gcgatacgaa gatcgtcggg tttccgaatt tcgctcc
//
LOCUS       DATDMP010000397         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_731612, whole genome shotgun sequence.
ACCESSION   DATDMP010000397 DATDMP010000000
VERSION     DATDMP010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731612"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VI195_06905"
     CDS             <1..711
                     /locus_tag="VI195_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6824152.1"
                     /translation="WASPNNPSGVVLNEDELAAIAELARRHELWLIVDEVYAGLAPGG
                     RVPGLAARLPGRVVTLGSLSKSHAMTGWRAGWLVGPRELVTHAENVAMCMLFGLPGFI
                     QEAVITALEHADEAEARIRAYCSARQARFAAGIAGVPSLKPLPPEAGMFMLIDVSGTG
                     LAGAGFVEALYAAQGVSVMDGAAFGGSTANCVRVCFATDEDTLDRACGRLREFCEHDL
                     PKKMRAASAARTDARNLR"
     gene            complement(787..1242)
                     /locus_tag="VI195_06910"
     CDS             complement(787..1242)
                     /locus_tag="VI195_06910"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEY6824153.1"
                     /translation="MHPTARTLRQRTCAALEPRPRFAGEYARVGTRAQEAGGIMGLLD
                     GLLGGAVGAEMATVVNSFIQQHGGVQGIVAQLEKQGMSATVRSWVGTGANQPISPDQV
                     HEVFGSGTIAQVAAKFGMNPQELAQKLSQVLPTAIDKLTPEGKVPPKTA"
     gene            1348..>2496
                     /locus_tag="VI195_06915"
     CDS             1348..>2496
                     /locus_tag="VI195_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY6824154.1"
                     /translation="MRQVRTLGLLGTGVIGGGWAARALHFGIDVIAADAKAHMEEWLR
                     GAVANAEGALARLTFAPLPPKGRLTFTTDLHAMARQADLIQENIPEQLELKQRVLADV
                     SHHAPADVVIASSTSGLMPSDLQRDMLAPERLLVAHPFNPVYLLPLVELVGGRATSEA
                     ALEAAARFYRFIGMHPLRVRREVPGHLTDRLQEALWREILHLVNEDVATTGELDESIV
                     YGPGLRWAGMGTNLIYHLAGGEPGMRHMLRQFGPCLKWPWTKLEAPELTEGLIDKLVA
                     GTQAQAAGRSIRELERLRDDYLVAIQQVLREYNIGAGATLRALEERLYENAAARARAR
                     IAAVSASAAAGNRSNPLRLLETQVRPEWIDYNGHMTDSRYLQVFGDASD"
BASE COUNT          392 a          829 c          865 g          410 t
ORIGIN      
        1 tgggcgagcc cgaacaaccc gagcggtgtg gtgctgaacg aggatgagct cgcggctatc
       61 gcggagctcg cgcggcgtca cgagctgtgg ttgatcgtcg atgaggtgta cgcgggtctc
      121 gcacctggcg ggcgcgtgcc aggcctcgct gcgcggctgc cggggcgagt cgtgacgctg
      181 ggcagcctgt cgaagtcgca tgccatgacc ggctggcgcg ccggttggct cgtcggcccg
      241 cgcgagctcg tcacgcacgc cgagaatgtc gccatgtgca tgctgtttgg actgccgggc
      301 ttcatccagg aagcggtcat cactgcgctc gagcacgcgg acgaggccga ggcacgcatc
      361 cgcgcctact gcagcgcgcg gcaggcgcgt ttcgcggcgg gcatcgccgg cgtgccgtcc
      421 ctcaagccgc tgccgccgga agcgggcatg ttcatgctga tcgatgtcag cggcactggc
      481 ctcgcggggg ccgggttcgt cgaggcgctc tatgcggcgc aaggcgtctc cgtgatggat
      541 ggggcggcct tcggcgggtc aacggccaac tgcgtgcgtg tgtgcttcgc caccgacgaa
      601 gatacgctcg atcgagcctg cggccgtttg cgggagttct gcgaacacga cctgccgaaa
      661 aaaatgcggg cggcaagcgc cgcccgcact gatgcccgca atctgcgctg aagtcgcagc
      721 agcgcgccgg cgccgccgcg cgcgctggcg cgcaggagtc tgctggagca ggccgcccga
      781 gccgggttaa gcggtcttcg gcggaacctt gccctcggga gtgagcttgt cgatcgcagt
      841 cggcagaacc tgcgagagct tctgcgccag ctcctgcgga ttcatgccga atttggcggc
      901 gacctgcgcg atcgtgccgc tgccgaacac ctcgtgcacc tgatccggtg agatcggctg
      961 gttcgctccc gtgccgaccc acgaacgtac ggttgcgctc ataccttgtt tctcgagctg
     1021 tgcgacgatc ccctggacgc cgccgtgctg ttgaatgaat gaattgacca ccgtggccat
     1081 ctcggccccg acggcgccac ccaacaatcc atctaacagg cccatgatgc ctcccgcttc
     1141 ttgcgctcgc gttccgacgc gcgcgtattc tccggcaaac cgcggccggg gctcaagggc
     1201 agcgcatgtg cgctgccgca gcgtccgcgc cgtggggtgc agacgctcat gacctggcgt
     1261 ggcgctctgt tatattatta tgacgacatt aaaaagaacc ggcacgggag cggcaacgac
     1321 gcagcaggtg gagcgacgga catcatcatg aggcaggtgc gaacgctggg tcttctgggc
     1381 acgggtgtca tcggcggcgg ctgggcagcc cgggcgctgc atttcggcat cgacgtcatc
     1441 gcggccgatg ctaaagcgca catggaggag tggctgcgcg gcgccgtcgc caatgccgaa
     1501 ggcgcgctgg ctcggctcac cttcgcaccc ctgccgccca agggtcggct caccttcacc
     1561 accgatctgc acgccatggc gcgccaggcg gacctgatcc aggagaacat ccccgagcag
     1621 ctcgagctga aacagcgggt gctggcggat gtcagccatc acgcgcccgc ggacgtcgtg
     1681 atcgcctcga gtacctcggg cctgatgccc tcagacctgc agcgcgacat gctggcgccg
     1741 gagcggctgc tggtcgctca tcccttcaac ccggtgtacc tgctgccgct ggtcgaactg
     1801 gtcggcggcc gcgccacttc ggaggcggcg ctggaggccg cggcgcgctt ctaccgcttc
     1861 atcggcatgc atcccctgcg ggtgcgccgc gaggtgccgg gtcacctcac cgaccgcctg
     1921 caggaggcgc tgtggcgcga gatcctgcac ctggtgaacg aggatgtcgc cacgaccggc
     1981 gagctcgatg aatccatagt gtacggtccc gggttgcgct gggcgggcat gggcaccaac
     2041 ctcatctacc atctcgccgg cggcgagccc ggcatgcggc acatgctgcg gcagttcgga
     2101 ccgtgtctga aatggccatg gacgaagctc gaagcgcccg agctcactga gggactgatc
     2161 gacaagctgg tggcgggcac ccaggcgcag gccgcagggc gctcgatccg cgagctcgag
     2221 cggctccgcg atgactacct cgtggcgatt cagcaggtgc tgcgcgaata caacatcggg
     2281 gccggcgcga cgctgcgcgc cctcgaagag cggctgtacg agaacgccgc ggcgcgcgca
     2341 cgggcacgca tcgccgcggt gtccgcctcc gccgcggccg gcaatcgcag caacccgctg
     2401 cggctgctcg agacacaggt gcggcccgag tggatcgatt acaacggtca tatgaccgat
     2461 tcgcgctatc tgcaggtgtt cggcgatgcg agcgat
//
LOCUS       DATDMP010000398         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1398133, whole genome shotgun sequence.
ACCESSION   DATDMP010000398 DATDMP010000000
VERSION     DATDMP010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398133"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..903
                     /locus_tag="VI195_06920"
     CDS             79..903
                     /locus_tag="VI195_06920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY6824155.1"
                     /translation="MQARLVIDGRELAVDLARPVGLALELDFAAPQPRHFGAPRASSR
                     PYETPGFDFRGSVERGSSCNCEMITLVPHCNGTHTECSGHLTRERLDAWRVTPPGLVP
                     AVLLSVAAEAPGAEGSDPAPQPGDRLITRRALERAWPAALPFEPRAVVIRTLPNGADK
                     RARDYTGQTPPYLSQEAAALLVSRGVLHLVVDVPSIDRAHDEGRLTAHRLFFGLPRGS
                     VRLAEATRPAATITELAFVPDVLADGAYLLELQVPALGGDAVPSRPLLYAVTQRAA"
     gene            900..>2017
                     /gene="kynU"
                     /locus_tag="VI195_06925"
     CDS             900..>2017
                     /gene="kynU"
                     /locus_tag="VI195_06925"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEY6824156.1"
                     /translation="MSSAHTLAAARARDARDPLAGYRERFALPHDASGVPLVYLCGHS
                     LGLLPLSARALVNEELEQWAHLAVQAHELGRRPWIPYHENLTAGLAALAGAQPDEVVA
                     MNSLTVNLHLMLASFYRPQGKRTRILMEAGAFSSDRHAVTSQIAWHGLDPRDALLELP
                     PPRGEATLPEELIEAYLARHARDIALVLWPGVQFRTGQAFDLERIARAAHAAGAIVGF
                     DLAHSIGNTPLALHAAGADFAVWCSYKYLNGGPGAIGGCFVHQRHSEAQPRTAHGGTL
                     PGTRLAGWWGHEEPTRFLMEPQFRAATGAAAWQISNPPILAAAPLIASLSIFLQAGME
                     ALRAKSVALTGFLEELLQPLAPEVQILTPRDPGRRGCQL"
BASE COUNT          268 a          754 c          686 g          309 t
ORIGIN      
        1 cggtgttcga gcgcttcttc gccagcgcgg cgcggcgcac ctgcgcccgc tgtcacacgg
       61 tcatggagcg ctcctgaggt gcaggcacgc ctggtgatcg acggccgcga gctcgccgtc
      121 gacctcgcgc gccccgtggg cctcgccctc gagctggatt tcgcggcgcc ccagccgcgg
      181 catttcggcg ctccgcgcgc gagctcgcgg ccgtacgaga cgcccggatt cgactttcgc
      241 ggctcggtcg agcgcggctc gagctgcaac tgcgagatga tcacgctggt cccgcactgc
      301 aacggcacgc ataccgaatg cagcggccac ctcacgcgcg agcgcctgga cgcatggcgc
      361 gtgacgccac caggcctcgt gccggcagtg ctgctgtcgg tagccgcgga agcccccggc
      421 gctgagggaa gcgacccggc gccgcagccc ggtgaccgct tgatcacacg ccgcgcgctc
      481 gagcgtgcct ggccggcggc cttgccgttc gagccgcgcg cagtcgtgat ccgcacactg
      541 cccaacggtg cggacaagcg tgcgcgcgac tacaccggcc agaccccgcc gtacctgtcg
      601 caggaggctg cggcgctgct ggtgagccgc ggcgtgctgc acctggtggt cgatgtgccc
      661 tcgatcgacc gcgcgcacga tgagggccgg ctcaccgccc accggctgtt cttcggtctg
      721 ccgcgtggca gcgtgcgcct ggccgaggcc acccgcccgg ctgcgaccat caccgagctc
      781 gctttcgtcc ccgacgtact cgccgacggc gcctatctcc tcgagctgca ggtgccggcg
      841 ctcggcggcg atgccgtgcc gagccggccg ctgctgtacg ccgtgacgca gcgcgcggca
      901 tgagctccgc gcacactctc gcggccgcac gcgcgcgcga tgcgcgcgat ccgctcgccg
      961 gctatcgcga gcgcttcgcg ctgccgcacg atgccagcgg ggtgccgctc gtgtacctct
     1021 gcggtcattc actcgggctg ttgccgctct cggcccgcgc gctggtgaac gaggagctcg
     1081 agcagtgggc gcaccttgcg gtgcaggcgc acgagctcgg tcgccgcccg tggatcccgt
     1141 atcacgagaa cctgaccgcc ggacttgcgg cactcgctgg tgcgcagccc gatgaggtgg
     1201 tcgccatgaa ctccctcacg gtgaacctgc acctgatgct ggcgagcttt tatcggccgc
     1261 agggcaagcg cacccgcatc ctcatggagg cgggcgcgtt ttcctccgat cggcacgcgg
     1321 tgacctcgca gatcgcctgg cacgggctcg atccgcgcga cgcgctgctc gagctgccac
     1381 cgccgcgcgg cgaagccacg ctgccggagg agctcatcga agcgtatctc gcccgccatg
     1441 cccgcgatat cgcgctggta ctgtggcctg gagtgcagtt tcgcaccggc caggccttcg
     1501 acctcgagcg catcgcgcgt gccgcgcacg ccgcgggggc catcgtgggc ttcgacctcg
     1561 cacactccat cggcaacacc ccgctcgcgc tgcacgcggc gggcgctgac ttcgccgtct
     1621 ggtgcagcta caagtaccta aatggcggac ccggcgcgat cggcggctgt ttcgtgcacc
     1681 agcgtcacag cgaggcccaa ccacgcacgg ctcatggcgg caccctgcca ggaacgcgcc
     1741 tggccggctg gtggggacac gaggagccga cgcgttttct catggagccg caattccgcg
     1801 cagcgaccgg ggcggcggcc tggcagatca gcaacccgcc gattctcgca gccgccccgc
     1861 tgatcgcctc gctgtcgata ttcctgcagg ccggcatgga ggcgttgcgt gcgaagtccg
     1921 tcgcgctcac cggtttcctg gaggagctgc tgcaaccgct cgcgccggag gtgcagatac
     1981 tgaccccacg cgaccccgga cggcgcggct gtcagct
//
LOCUS       DATDMP010000399         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1841848, whole genome shotgun sequence.
ACCESSION   DATDMP010000399 DATDMP010000000
VERSION     DATDMP010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841848"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VI195_06930"
     CDS             complement(<1..194)
                     /locus_tag="VI195_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824157.1"
                     /translation="MRTLFLLLVAVNLALFAWFQYYSPVESATDPEPARRQLNPDKIR
                     LLEGKELKSLASTRPPPASAA"
     gene            complement(199..933)
                     /locus_tag="VI195_06935"
     CDS             complement(199..933)
                     /locus_tag="VI195_06935"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY6824158.1"
                     /translation="MILAIDAGNSRVKWGWYEPDSPGVWSSIANVSLIEFAASSDHIN
                     PFSATHENPERILISNVAGEGAHQLIVNWTSIFEAAPEWIHGEREQCGVRNLYQRPEQ
                     LGPDRWASLIAARALEPGRACLVVNAGTCTTVDMLSAAGEFTGGLILPGVDLMRFVLP
                     EHTGRLPLQEGRFVAEPRSTLDAIESGCRHAQAGAVERMFRGMGRGATCVVSGGGGPS
                     LIERVDLPCRYVENLVLEGLARIGTA"
     gene            complement(930..1550)
                     /locus_tag="VI195_06940"
     CDS             complement(930..1550)
                     /locus_tag="VI195_06940"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY6824159.1"
                     /translation="MDRLDASQISVPGLQARVVDRCTSTNTLLREERSAGPALLAAEL
                     QTAGRGRHGRRWRSARGAGATFSSRRPMRCEQRRLVGLSVAVGLSSARALRRLGARGV
                     ALKWPNDLMVNKRKLGGILIETRPLPRPDDRPAAPGSVVIVGIGINCRTQPRFGARLG
                     RGVAALDEVLRRPLSRNAVIGAVAREVLGALAVWERAWERAGGHAP"
     gene            complement(1561..>2136)
                     /locus_tag="VI195_06945"
     CDS             complement(1561..>2136)
                     /locus_tag="VI195_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6824160.1"
                     /translation="EDIARMRAGALRPLRRKVQIVFQDPYRSLNPRRTVGEAITEGPR
                     NYGLPHAEALTRARKLLDLVRMDASALERYPHQFSGGQRQRICIARALAMEPELLIAD
                     EAVSALDVSVQAQVLALLEEIRERLNLAMLFITHDLRVAAQVCDHVAVMSRGRVVEYG
                     AAEEVFLNPRHEYTRALFNAAPGRNFAFGST"
BASE COUNT          311 a          769 c          762 g          294 t
ORIGIN      
        1 gcggcggagg ccggcggcgg tctggtcgag gcgaggctct tcagctcctt gccctcgagc
       61 aggcggatct tgtccgggtt gagctggcgg cgcgccggct cggggtcggt ggcgctctcg
      121 accggcgagt agtactggaa ccatgcgaac agcgcgagat tcaccgccac cagcaggaga
      181 aaaagggttc tcatccgctc acgccgtgcc gatccgcgcc agtccttcga gcaccagatt
      241 ctccacgtag cggcagggca ggtcgacgcg ctcgatgaga gagggcccgc cgccgcccga
      301 cacgacgcag gtcgcgcccc gtcccatgcc gcggaacatg cgctccaccg cgcccgcctg
      361 ggcgtgccgg cagccgctct cgatcgcatc cagggtgctg cgcggttcgg cgacgaagcg
      421 tccttcctgc agcggcaggc ggcccgtatg ctcgggcagc acgaagcgca tgaggtcgac
      481 ccccggcagg atcagcccgc ccgtgaattc gcctgcggcc gagagcatgt ccaccgtggt
      541 gcaggtgccg gcgttcacca ccaggcaggc gcggcccggc tccagcgcgc gcgccgcgat
      601 gagcgaagcc cagcggtccg gcccgagctg ctcgggccgc tggtagaggt tgcgcacgcc
      661 gcactgctct cgctcgccgt ggatccactc gggcgcggcc tcgaagatgc tggtccagtt
      721 gacgatgagc tggtgcgcgc cctcgccggc gacgttggag atgaggatgc gctccgggtt
      781 ctcgtgcgtc gccgagaacg ggttgatgtg gtcgctcgag gcggcgaact cgatgagcga
      841 cacgttggcg atgctcgacc acacgcccgg cgagtccggc tcgtaccagc cccatttcac
      901 gcgcgagttg cccgcgtcga tggcgaggat catggcgcat gccccccggc ccgttcccag
      961 gcgcgctccc agacggcaag cgcgcccagc acttcgcggg cgaccgcgcc gatcacggcg
     1021 ttgcgcgaga gcgggcgccg cagcacctcg tccagcgcgg cgacgccacg gcccaggcgc
     1081 gcgccgaatc gcggctgcgt tcggcaattg atgccgatgc cgacgatcac cacgctgccc
     1141 ggcgccgcag gccggtcgtc agggcgcggc aggggccgcg tttcgatcag gatgccgccg
     1201 agcttgcgct tgttcaccat caggtcgttg ggccatttca gcgcgacgcc ccgcgcgccg
     1261 agcctgcgca gcgcgcgcgc cgaggagagg cccaccgcga ccgagagccc gaccagccgg
     1321 cgctgctcgc agcgcatggg ccggcggctc gaaaaggtcg cgccggcgcc gcgcgccgag
     1381 cgccagcgcc gcccatgccg gccgcggccg gcggtctgca gctcggcggc gagcagcgcg
     1441 ggtccggcgg accgctcctc ccgcagcagc gtgttggtcg aggtgcagcg gtccacgacc
     1501 ctggcttgga ggcccggaac ggaaatctgc gacgcgtcga gccggtccat gatgcggatt
     1561 ctacgttgaa ccgaaagcga aattccgtcc cggcgccgca ttgaacagcg cgcgcgtgta
     1621 ttcatggcgc ggattgagga acacctcctc ggcggcgccg tactcgacca cgcgcccccg
     1681 cgacatcacc gcaacgtggt cgcagacctg cgcggccacg cgcaggtcgt gcgtgatgaa
     1741 cagcatcgcg agattcaggc gctcgcggat ctcctccagc agcgcgagca cctgcgcctg
     1801 cacggacacg tcgagcgccg acaccgcctc gtcggcgatg agcagctcgg gctccatcgc
     1861 cagcgcgcgc gcgatgcaga tgcgctggcg ctggccgccg gaaaactggt gcgggtagcg
     1921 ctcgagcgcg gacgcgtcca tgcgcaccag atcgagcagc ttgcgcgcgc gtgtgagggc
     1981 ctctgcgtgc ggcagcccgt agttgcgcgg cccctccgtg atggcctcgc cgaccgtgcg
     2041 gcgcggattg agcgagcgat aggggtcctg gaacacgatc tgcaccttgc ggcgcagcgg
     2101 ccgcagcgcg cccgcgcgca tgcgcgcgat gtcctc
//
LOCUS       DATDMP010000400         3841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1287332, whole genome shotgun sequence.
ACCESSION   DATDMP010000400 DATDMP010000000
VERSION     DATDMP010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287332"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VI195_06950"
     CDS             <1..660
                     /locus_tag="VI195_06950"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824161.1"
                     /translation="PRFARKPPQVRRQALIDATIECLKRYGHEGLSIRTISAQAGVSV
                     GLINHHFPNKDELVAAAYRHINSGLVLGMQAAVARAGNSPRARMRAFLEAWFSAPSLD
                     ADALAVWVVFWGLYRHSRLIQRAHRETYQGYVTLLRGLLAELLPQRRASRHARTGRRR
                     RGAGTAVVDLRLAAIGFTALLDGLWLEWCLEPGTFRPAEAVELCEAWIASLSRPSAAH
                     R"
     gene            complement(666..1574)
                     /locus_tag="VI195_06955"
     CDS             complement(666..1574)
                     /locus_tag="VI195_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY6824162.1"
                     /translation="MAGTDTTRAAPPAQRRLPSVLGWMPVQLLLLFISLAAIDVACQL
                     LGARLVHAAPAPLRVVARLSTALVLSLAMITAYRSLVGVLERRTAHELQQAGAARALA
                     VGASGGAALFALVYLILWAAHAATYGPFNGFGGLDHALAVAIASAVGEEIVFRGVVFR
                     RLEGSLGTSIALIFSAAVFGLVHAGNAGASWISTLAIALESGVLLGVAYAATRTLWLP
                     IGVHFGWNFTEGGIFGAAVSGGQYQGLIATPLSGPAWLTGGAFGPEASIPALIVSLCA
                     SAALAWYALQRGTWRPAPWRLRAQLQ"
     gene            1809..3659
                     /locus_tag="VI195_06960"
     CDS             1809..3659
                     /locus_tag="VI195_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6824163.1"
                     /translation="MATAVHPPRASDRAASAEGLTLRGFFGVFRYSRRALGLVWSTNR
                     ALTVALALLTLLAGILPASVAYVGALIVDAVLAAIRLGGSAGHVLRLVVLEGVLVAAI
                     AAAQRGLSLCQSLLRAQLGQRVNVMILEKALTLELQHFEDSEFYDKLTRARREASTRP
                     LSLVTRTFGLVQNGVSLASYGVLLAHFSPWAVAVLLLAGLPAFVAEAKFSGDAFRLFR
                     WRSPETRMQIYLETVIAREDHAKEVMLYHLGPRLLERYRDIFRRLYAEDRALTVRRDL
                     WGFALGSIATAALYGAYAWIAITTVRRIITLGQMTMYVALFRQGQAAVSAMLAAVGGM
                     YEDNLYLSTLYEYLETHVPRPKGTVLHGPHPEDGVRFEDVSFSYPGAEEPALEHISLH
                     LTPGSSLALVGENGSGKPTLIKLLTRLYAPSSGKILLDGQDLAAWEEGALRERIGVIF
                     QDFARYQMLVGENVGAGDERFFEDEARWRVAAHKGQASEFIDTLPAGYQTQLGKWFRD
                     GRELSGGQWQKIALSRAFMRTRADILVLDEPTAAMDAEAEAEVFEHFRQLAHDRITIL
                     ISHRFSTVRMADQIVVLNRGRIVERGSHDELMRLNGRYAHLFSLQARGYR"
BASE COUNT          587 a         1317 c         1309 g          628 t
ORIGIN      
        1 ccgcgctttg cccgcaagcc tccgcaggtg cgccgtcagg cgttgatcga cgccaccatc
       61 gagtgcctga agcgctacgg ccatgagggc ctctcgatcc gcaccatcag cgcgcaggcg
      121 ggcgtgtcgg tcggcctcat caaccatcac ttccccaaca aggacgagct ggtggcggcg
      181 gcttatcgcc acatcaactc cgggctcgtc ctcggcatgc aggcagccgt cgcgcgcgcc
      241 ggcaattcgc cccgcgcgcg catgcgcgcc ttcctggaag cctggttctc cgcgccgagc
      301 ctcgatgccg atgcgctcgc ggtgtgggtg gtgttctggg gcctctaccg ccactcgcgc
      361 ctcatccaac gcgcgcaccg cgagacctat cagggttacg tgactctctt gcgtggcctg
      421 ctggcggagc tgctgccgca acggcgtgcc tcacgccatg cccgcaccgg gcgccgccgc
      481 cgtggcgccg gcacggcggt ggtcgatctg cgcctcgccg ccatcggttt cactgcgctg
      541 ctcgatggcc tgtggctcga gtggtgcctc gagcccggga cgttccgccc ggcggaggcc
      601 gtggaattgt gcgaggcgtg gatcgcaagc ttatcgcgcc cgtccgcagc gcatcgctag
      661 caccactatt gcaactgcgc cctcagccgc cacggcgcag gccgccaggt gccgcgctgt
      721 agtgcgtacc aggcgagcgc cgccgaggcg cacagcgaga cgatgagcgc cgggattgaa
      781 gcttccgggc cgaacgcgcc gccggtcagc caggcgggcc cggacaaggg agtggcgatc
      841 agaccctggt actgtccgcc tgagaccgcg gcaccgaaga tgccgccttc ggtgaaattc
      901 cagccgaaat gcacgccgat cggcagccac agcgtgcgcg tcgcggcgta ggcgacgccg
      961 agcagtacgc cgctttcgag cgcaatcgcg agcgtcgaga tccagctcgc accggcgttg
     1021 ccggcgtgca ccagcccgaa gacagccgcg gagaagatga gcgcaatgct ggttccgagc
     1081 gagccttcca gccggcggaa caccactccg cggaacacga tctcttcccc gactgccgag
     1141 gcgatcgcca cggcgagcgc atgatcgaga ccgccgaatc cgttgaaggg gccgtaagtg
     1201 gcggcgtgcg ctgcccacag gatcaggtag acgagcgcaa acagcgccgc accgcccgat
     1261 gcacccacgg cgagtgcgcg cgccgcaccc gcctgctgca gctcatgggc agtgcgccgc
     1321 tcgagcacgc cgacgagcga gcggtaggcg gtgatcatcg ccagcgacag gacgagcgcc
     1381 gtcgagagcc gggccacgac gcgtagcggc gcgggcgccg cgtgcacgag ccgcgcgccg
     1441 agcagctgac aggcaacatc gatggccgcg aggctgatga acagcagtag cagctgcacc
     1501 ggcatccagc cgagcacgct cggcaggcgg cgctgtgccg gcggggcggc acgggtggta
     1561 tcggtgcccg ccatgttgtt gtttctccct gggagacgcc ccgggtgagc attatgcgcg
     1621 ctcgcgtgcc gccagtggcc gcgcggactt gcggccaccg ataatggccc cacattttcg
     1681 atctgtgcag gcgaggacat gttgagccag agggagctgc gttaccgggc gggcatcatc
     1741 gatgcggagc tgcctggcga tcgtagcgcg cgcctcatta cccgcgtcgg ttccctgggg
     1801 gagcgctgat ggcaaccgcc gtacatccgc cgcgtgcgag cgaccgggcg gcgtctgcgg
     1861 agggcctcac gctgcgcggg ttcttcggcg tattccgtta cagccgccgg gcgctcgggc
     1921 tcgtctggag caccaaccgc gcgctcaccg tggcgctcgc cctgctcacg ctgctcgcgg
     1981 gcattctgcc ggcatcggtc gcgtacgtgg gcgcactgat cgtcgatgcg gtgctcgctg
     2041 cgattcgact cggcggcagc gccggccacg tgctgcgcct cgtggtgctc gagggcgtac
     2101 tggtcgcagc gatcgccgcc gcgcaacgtg gcctgtcgct gtgccagtcg ctgctgcggg
     2161 cacagctcgg gcagcgcgtc aatgtcatga tcctcgagaa ggcgttgacc ctcgagctgc
     2221 agcacttcga ggactcggag ttctacgaca agctcacgcg cgcgcgccgc gaagcctcga
     2281 cgcggccgct gagcctggtc acgcgcacct tcggactcgt gcagaacggc gtttcgctcg
     2341 ccagctacgg ggtgctgctc gcgcacttct cgccctgggc ggtcgccgtg ttgctgctcg
     2401 ccggcctgcc ggctttcgtg gcggaggcga aattctccgg tgatgcgttc cggctgttcc
     2461 gctggcgctc gcccgagacg cgcatgcaga tctacctgga gacggtgatc gcccgcgagg
     2521 atcacgccaa ggaggtcatg ctctatcacc tcggcccccg cctgctcgag cgctaccgcg
     2581 atatcttccg ccgcctgtac gcggaagatc gtgccctgac cgtgcgccgc gatctgtggg
     2641 gcttcgcgct cggctcgatc gccaccgccg cgctctatgg agcgtatgcc tggatcgcga
     2701 tcaccactgt gcggcggatc atcacgctcg gccagatgac catgtacgtg gcgctgttcc
     2761 ggcaggggca ggcggcggtg tcggcgatgc tggccgcggt gggtggcatg tacgaggaca
     2821 acttgtatct ctcgaccctg tacgagtacc tcgagacgca cgtgccgcgc cccaagggca
     2881 ctgtgctgca cggtccgcat cccgaggatg gcgtgcgctt cgaggacgtc agcttcagct
     2941 atcccggggc cgaggagccg gcgctcgagc acatcagcct gcacctcacc cccggcagca
     3001 gtctcgccct cgtcggtgag aacggctccg gcaagcccac gctgatcaag ctgttgacac
     3061 gactgtacgc gccgagctcg ggcaagatcc tgctcgatgg ccaggacctc gccgcgtggg
     3121 aggaaggtgc gctgcgcgag cgtatcggcg tcatcttcca ggatttcgcg cgttatcaga
     3181 tgctggtcgg agagaatgtc ggcgcaggcg atgagcgctt cttcgaggac gaggcgcgct
     3241 ggcgcgtcgc cgcgcacaag gggcaggcga gcgaattcat cgataccctg ccggccggct
     3301 atcagacgca gctcggcaaa tggttccgcg acgggcgcga gctttccgga ggccaatggc
     3361 agaagatcgc gctgtcgcgc gccttcatgc gcacccgtgc cgatattctc gtgctcgatg
     3421 agccgactgc ggcgatggat gccgaggcgg aggcggaggt attcgagcac ttccgccagc
     3481 tcgcgcacga ccgcatcacc atcctgatct cgcaccgctt ctcgaccgtg cgcatggcgg
     3541 atcagatcgt ggtgttgaat cgcggccgca tcgtcgaacg cggcagccac gatgaactga
     3601 tgcgcctcaa cgggcgctac gcgcacctct tcagcctgca ggcgcgcggc tatcgttgac
     3661 tccgtaggga ccttggcgaa cgggttatcg ataacggctg cgaacgggct agcgctgagc
     3721 agacggctgc tggccctgca ggcgcgcctg cgcccgcatg cggtgcacat cgagcgggtg
     3781 gatctcatcg atgatgcatt gccaggtgcc gccgacgccg cttctcacct cgatcgagtc
     3841 g
//
LOCUS       DATDMP010000401         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_65226, whole genome shotgun sequence.
ACCESSION   DATDMP010000401 DATDMP010000000
VERSION     DATDMP010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65226"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VI195_06965"
     CDS             <1..525
                     /locus_tag="VI195_06965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6824164.1"
                     /translation="EPTEALRIFRELMFALGEIHEKGILHRDLKPQNLMFRHDGTLAI
                     LDFGIAKHIDAIDKTRHGEVLGTPRYMSPEQVQGRALDLRTDIYSAGVLLYQMLTGRH
                     LFEGDTAVEVALHHLNTPPPALPEHLQQYQRLLDKLLEKDREARFRNADEVLGFLTRK
                     FQQNPALERTVRLH"
     gene            complement(527..916)
                     /locus_tag="VI195_06970"
     CDS             complement(527..916)
                     /locus_tag="VI195_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6824165.1"
                     /translation="MPDRPLNRICYVEDDEDIQRIVRMSLERVGKMTVEVVSDPNQAI
                     GVMARFKPDLVMLDWMMPGMDGPTLFRQMKLDPQTASLPVVFITAKATQRDMEELISL
                     GAAATISKPFSPKDLPDQLRAIWAKLP"
     gene            complement(909..>2036)
                     /locus_tag="VI195_06975"
     CDS             complement(909..>2036)
                     /locus_tag="VI195_06975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY6824166.1"
                     /translation="SGYRLRHAYQRSLVELDEARRQIEASGRKLALFAERSPIAVFEL
                     DGEGVLREVNQAAEILFGFAADELVGRSVKRLVLARHHADFDREWQRLIASRQPATGV
                     KVRNLRRDGLTVVCEWTVTPLINAEGALISVIAQGHDVTAQLEAERMKKEFTSTLSHE
                     LRTPLTSIIGSLQLINAGVLGEVTKDVMDLTEVAERNGQRLLDLINDILDIEKIDSGK
                     LTLNPEVLSVDDLVREAIVLNKGFADRFKVRFESHGELVRRQVNADRKRLLQVMTNLL
                     SNAAKFSPEGAAVQITTEDRGAWLRIAVNDRGPGIPDGFRARIFGRFTQADSTTSRHK
                     GGTGLGLAICKRLIEMMQGRIGFEDRPEGGTTFWFELPRHA"
BASE COUNT          346 a          680 c          658 g          352 t
ORIGIN      
        1 gagccgaccg aggcgttgcg catcttccgc gagctgatgt ttgcgctcgg cgagatccac
       61 gagaagggca tcctgcatcg cgacctgaaa ccgcagaacc tgatgttccg ccacgatggg
      121 acactcgcga tcctcgactt cggcatcgcc aagcacatcg acgccatcga taagacgcgt
      181 cacggcgagg tgctcggcac gccgcgctac atgagcccgg agcaggtgca gggccgtgcg
      241 ctcgacctgc gcaccgacat ctacagcgcc ggcgtgctgc tctaccagat gctcacgggg
      301 cggcacctct tcgagggcga cacggcggtc gaggtggcgc tgcaccacct gaacacgccg
      361 ccgcccgcgc taccggagca cctgcagcag tatcagcggc tgctcgacaa gctgctggaa
      421 aaggaccgcg aggcgcgctt ccgcaacgcc gacgaggtgc tcggcttcct gacgcgcaag
      481 ttccagcaga accctgctct cgagcgtact gtaaggctgc actgagctag ggtagcttcg
      541 cccagatcgc gcgcagctgg tccggcaggt ccttgggcga aaacggcttg gagatagtgg
      601 cggccgcgcc gagcgagatc agctcctcca tgtcgcgctg cgtcgccttg gcggtgatga
      661 acaccaccgg cagggatgcg gtctgcgggt ccaacttcat ctggcgaaaa agcgtcggac
      721 catccatgcc cggcatcatc cagtcgagca tcaccaggtc cggcttgaag cgcgccatga
      781 cgccgatcgc ctggttcgga tcgctcacca cctcgaccgt catcttgccg acccgttcga
      841 gcgacatgcg cacgatgcgc tggatgtcct cgtcgtcctc gacgtagcag attcggttta
      901 gcgggcggtc aggcatggcg gggtagctcg aaccagaaag tggtgccccc ttcggggcgg
      961 tcctcgaagc cgatgcggcc ctgcatcatc tcgatcagcc gcttgcaaat agcgagcccg
     1021 agcccggtgc cgcccttgtg gcgcgaggtg gtggaatcgg cctgcgtgaa gcgcccgaag
     1081 atgcgtgcgc ggaacccgtc gggaatgccc ggcccgcggt cgttcaccgc gatccgcagc
     1141 cacgcgccgc gatcctcggt ggtgatctgc accgccgcgc cctcgggcga gaacttggcg
     1201 gcgttggaaa ggaggttggt catcacctgc aggagccgct tgcggtcggc attgacctgg
     1261 cgacggacga gctcgccgtg cgattcgaag cgcaccttga agcgatcagc gaagcctttg
     1321 ttcagtacga tcgcctcgcg caccaggtcg tccacgctga gcacctccgg attgagcgtc
     1381 agcttgcctg aatcgatctt ctcgatgtcg aggatgtcat tgatcaggtc gagcaggcgc
     1441 tggccgtttc gctctgcgac ctcggtgagg tccatgacat cctttgtcac ttcgccgagc
     1501 acaccggcat tgatgagctg cagcgagccg atgatcgagg tgagcggcgt gcgcagctcg
     1561 tgcgacaggg tcgaagtgaa ctccttcttc atgcgctcgg cctcgagctg cgccgtgacg
     1621 tcgtgtccct gtgcgatcac cgaaatgagc gcgccctcgg cgttgataag cggcgtcacg
     1681 gtccattcgc acacgacggt gaggccgtcg cgccgcaggt ttcgtacctt gacgccggtg
     1741 gcgggctgcc gcgaggcgat caggcgctgc cattcgcgat cgaagtccgc gtggtgcctc
     1801 gcgagcacca ggcgcttcac cgagcggccc accagctcgt cggcggcaaa gccgaacagg
     1861 atctccgcgg cctggttcac ctcgcgcagc acgccctcgc cgtcgagctc gaacaccgct
     1921 atcggcgagc gctcggcaaa gagcgcgagc ttgcggccgc tcgcttcgat ctgccgccgc
     1981 gcctcgtcca gctccaccaa gctcctctgg taagcgtggc gcagccggta gccgga
//
LOCUS       DATDMP010000402         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_620693, whole genome shotgun sequence.
ACCESSION   DATDMP010000402 DATDMP010000000
VERSION     DATDMP010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620693"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VI195_06980"
     CDS             complement(<1..573)
                     /locus_tag="VI195_06980"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY6824167.1"
                     /translation="MSIEILVNVAPSETRAAILESGVLQELYIERTSRRGLVSNLYKG
                     RVSRVLPGMQAAFVEIGLERTAFLHAADIAHSSATDTLANSAPTSLPQVEDIRRLVNS
                     GDDILVQVIKDPIGTKGARLTTFVALPSRYLVYMPRGEGVGVSARIEDEPERTRLKSM
                     LAELSGADPAGGGYIVRTAAQGATAENLRED"
     gene            complement(570..1190)
                     /locus_tag="VI195_06985"
     CDS             complement(570..1190)
                     /locus_tag="VI195_06985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY6824168.1"
                     /translation="MPPSAFHAPLLCLASASPRRRELLEQLGVPYVSAIPDIDETVLP
                     GESALDYVVRMARAKARIVHPHGAGLPVLAADTTVLIEQRICGKPADAEEGIAMLLRL
                     SGRSHEVLTAVALAAAGELNHRVSRSEVLFRHLTLAEAMAYWGTGEPRDKAGGYAVQG
                     HGAVFVEHLSGSYSGVMGLPLYETAELLAGAGVSYWLDRSVSGATP"
     gene            complement(1190..1657)
                     /gene="rlmH"
                     /locus_tag="VI195_06990"
     CDS             complement(1190..1657)
                     /gene="rlmH"
                     /locus_tag="VI195_06990"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY6824169.1"
                     /translation="MRVRVIAVGTRMPAWVRSACAEYLQRLRAGLPLELEEIDTASRR
                     AGGTPRALAREGERLLAALEPHEYVIALDERGRELTTQELARWLAARMQQGEDLAFVI
                     GGPDGLAPAVLARCNLRLALSRLTLPHALARVLLTEQLYRAHCILANHPYHRD"
     gene            complement(1667..>2039)
                     /gene="rsfS"
                     /locus_tag="VI195_06995"
     CDS             complement(1667..>2039)
                     /gene="rsfS"
                     /locus_tag="VI195_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY6824170.1"
                     /translation="HAVTTALEDMKAVNVRVLDVRGLTDIADTMVIASGNSDRHVRSI
                     AERVVERAKAAGFRPLGTEGERDGEWVLVDLQDVVVHVMLPRVREFYGLERLWDSGPA
                     LPAVAVPAAPRIAGRRRQPPR"
BASE COUNT          310 a          730 c          706 g          293 t
ORIGIN      
        1 gtcctcgcgc agattctcgg ctgtggcccc ctgcgccgcc gtgcgcacga tgtagccccc
       61 gccggcgggg tccgcgccgc tgagctcggc gagcatgctt ttcagccgcg tgcgctccgg
      121 ctcgtcctcg atgcgcgccg acacgcccac accctccccg cgcggcatgt acaccaggta
      181 gcgcgagggc agcgcgacga aagtcgtgag ccgggcgccc ttggtgccga tcgggtcctt
      241 gatcacctgc accaggatgt catcgccgct gttgaccagc cggcggatgt cctcgacctg
      301 gggcaggctc gtcggggcgg agttggccag cgtatcggtc gccgagctgt gcgcgatgtc
      361 ggccgcatgc aggaaagcgg tgcgctcgag gccgatctcc acgaacgctg cctgcatgcc
      421 cggcagcacg cgcgacacac gccccttgta gagattgctc accagcccgc ggcggctggt
      481 gcgctcgatg tacagctcct gcagcacgcc gctctcgaga atcgccgcgc gcgtctcgct
      541 cggcgccacg ttcaccagga tctcgatgct cacggcgtgg ctccgctcac cgagcggtcg
      601 agccagtacg acacgccggc gcccgccagg agttccgcag tctcgtagag cggcaacccc
      661 atgacacccg aatagctgcc cgagaggtgc tcgacgaaca cggcgccgtg gccctgcacg
      721 gcgtagccgc cggccttgtc gcgcggttcg ccggtacccc agtacgccat ggcctcggca
      781 agcgtcaggt gccggaaaag cacctcgctg cggctcacgc ggtggttcag ctcgccggct
      841 gccgccagcg cgacggccgt gagtacctcg tggctgcgcc cggagagccg caacagcatg
      901 gcgatgccct cctcggcgtc ggccggcttg ccgcagatac gctgctcgat gagcaccgtg
      961 gtgtcggcgg ccagcaccgg aaggcccgcg ccatggggat ggacgatgcg cgccttggcg
     1021 cgcgccatgc gtacgacata atccagcgcc gactccccgg gcagcacggt ttcatcgata
     1081 tcgggaatgg cggacacgta cggcacgccg agctgctcga gcagctcgcg ccgccgcggt
     1141 gaggccgagg cgaggcacag aagcggcgca tggaacgcgg atggcggcat cagtcgcgat
     1201 gatacgggtg attggcgagg atgcagtggg cgcggtacag ctgctcggtc aagaggaccc
     1261 gggcgagggc atgcggcagg gtgaggcggg acagcgcgag tctgagattg cagcgcgcca
     1321 gcactgccgg agcgagccca tcggggccgc cgatgacaaa agccaggtcc tcgccctgct
     1381 gcatgcgggc agcgagccag cgcgccagtt cctgcgtagt gagctcgcgg ccacgctcat
     1441 cgagcgcgat cacgtactcg tgcggctcga gcgctgccag cagacgctcg ccttcgcgcg
     1501 ccagcgcgcg cggtgtgccg ccggcgcgcc gcgaagccgt gtcgatttcc tcgagctcga
     1561 gcggcagacc cgcgcgtagc cgctgcagat actcagcgca ggcgctgcgc acccaggcag
     1621 gcatgcgcgt gccgaccgca atcacgcgca cgcgcatccg gcgagctcac cgcggtggct
     1681 gccgccgccg tccggctatg cgcggtgccg ccgggacggc gaccgccggc agcgccgggc
     1741 cgctgtccca gagccgctcg aggccataga actcccgcac ccgaggcaac atcacgtgca
     1801 ctaccacatc ctgcaggtcg acgagcaccc attccccgtc gcgctcgcct tcggtgccga
     1861 gtggccggaa gcctgccgcc ttggcgcgct cgaccacgcg ctcggcgatc gagcgcacgt
     1921 ggcgatcgga gttgccggag gcgatgacca tggtgtcggc gatgtcggtg aggccgcgca
     1981 catcgagcac cctcacgttt acggctttca tgtcctcaag cgcggtcgtc acggcgtgc
//
LOCUS       DATDMP010000403         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1732221, whole genome shotgun sequence.
ACCESSION   DATDMP010000403 DATDMP010000000
VERSION     DATDMP010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732221"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..179
                     /locus_tag="VI195_07000"
     CDS             15..179
                     /locus_tag="VI195_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY6824171.1"
                     /translation="MHEFSPGEVLIADMTDPDWEPVMKRAAAIVTNRGGRTCHAAIIA
                     RELGIPAVVG"
     gene            216..1259
                     /locus_tag="VI195_07005"
     CDS             216..1259
                     /locus_tag="VI195_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY6824172.1"
                     /translation="MTVSCAEGDNGFVYEGLLEFERKQVELDSLPKIPVKIMMNVGNP
                     DRAFDFAAIPNHGVGLARLEFIINRMIGVHPKALLEFERLDADLKEQIRTQMAGYSDP
                     VGFYVEKLAEGIAQIAAAFAPEPVIVRLSDFKSNEYANLIGGARYEPHEENPMLGFRG
                     ASRYLDESFRPCFELECRAIHRVRADMGLSNVQVMVPFVRTLTEARAVTELLAANGLT
                     RGGELRIIMMCELPTNALLAEQYLQYFDGMSIGSNDMTQLTLGLDRDSAIVAQGFDER
                     DEAVRRLLAMSIAACRKLGKYVGICGQGPSDHPDLARWLLDQGIESMSLNPDTVVETW
                     LFLAQPGAAGKTN"
     gene            complement(1302..1700)
                     /locus_tag="VI195_07010"
     CDS             complement(1302..1700)
                     /locus_tag="VI195_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6824173.1"
                     /translation="MSEELIRHRGGCHCGAVAFEVEAPARVTVSECNCSICRMSGFLH
                     LIVPRARLHLLRGADALTEYRFNTGSARHLFCRRCGVKSFYVPRSNPDGYSVNLRCLE
                     RSTLMEVTIESFDDNDRAAAEARVRDRSRA"
     gene            complement(1693..2256)
                     /gene="orn"
                     /locus_tag="VI195_07015"
     CDS             complement(1693..2256)
                     /gene="orn"
                     /locus_tag="VI195_07015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY6824174.1"
                     /translation="MPSGDYLVWIDLEMTGLKPDSDVIIEIATVVTDKDLAIFAEGPV
                     LAIHHNDEVLARMDEWNKRQHGSSGLLARVRASRVSVAEAQQRTLEFLAPLVSAGSSP
                     MCGSSICQDRRFLARYMPDLERFFHYRNLDVSTLKELARRWAPPVAEGFVKQGTHLAL
                     ADIHESIRELRHYRARLLAPAWSGGIV"
     gene            2336..>2672
                     /locus_tag="VI195_07020"
     CDS             2336..>2672
                     /locus_tag="VI195_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824175.1"
                     /translation="MSQLVLGSVIATFGRHMVVRTADGAELRARPFGRTLEVVCGDEV
                     RCRVDPHHDEVHVIEVRPRRNGLWRANSRGGAEPVVANLTRLLVVLAPVPLPELFVID
                     RYLAAATAAG"
BASE COUNT          433 a          884 c          924 g          431 t
ORIGIN      
        1 cctcggtcga ccagatgcac gagttcagcc ccggcgaggt gctcatcgcc gacatgaccg
       61 accccgactg ggagccggtc atgaagcgcg ctgcggcgat cgtcaccaac cgtggcggcc
      121 gcacctgtca cgcggcaatc atcgcgcgcg agctcggcat ccctgcggta gtcggctgag
      181 gcgatgccac gcagcgcatc cgcgaggggc aggaggtgac tgtgtcctgc gccgagggtg
      241 acaacggctt cgtctacgag gggctgctgg agttcgagcg caagcaggtc gagctcgact
      301 cgctgccgaa gatcccggtg aagatcatga tgaacgtcgg caaccccgac cgcgccttcg
      361 acttcgccgc catccccaat cacggcgtgg ggctggcgcg gctcgagttc atcatcaacc
      421 gcatgatcgg ggtgcacccg aaggcgctgc tcgagttcga gcggctggat gcggacctca
      481 aggagcagat ccgcacgcag atggccggct actcggaccc ggtcggcttc tacgtggaga
      541 agctcgcgga aggcatcgcc cagatcgcgg cggcgttcgc tcccgagccg gtcatcgtgc
      601 gtctgtcgga cttcaagtcg aacgagtacg ccaatctcat cggcggtgcg cgctacgagc
      661 cgcacgagga gaacccgatg ctcgggttcc gcggcgcttc gcgctacctc gatgagagct
      721 tccgcccgtg cttcgagctc gagtgccgtg ccatccaccg cgtgcgcgcc gatatgggac
      781 tcagcaacgt gcaggtgatg gtgccattcg tgcgtaccct cacggaagcg cgcgcggtca
      841 ccgagctgct cgcagcgaat ggcctcacgc gcggcggcga gctgcgcatc atcatgatgt
      901 gcgagctgcc gaccaacgcg ctgctcgcgg agcagtacct gcagtatttc gatggcatgt
      961 cgatcggctc gaacgacatg acgcagctca ccctcggcct cgaccgcgac tccgccatcg
     1021 tggcgcaggg cttcgatgag cgcgatgaag cggtgcggcg tctgctggcg atgtccattg
     1081 ccgcctgccg caagctcggc aagtacgtcg gtatctgcgg ccagggtcct tcggaccatc
     1141 cggacctcgc gcgctggctg ctcgatcagg gcatcgagag catgtcgctc aatccggata
     1201 cggtggtgga gacctggctg ttcctcgcgc aacctggcgc tgccggcaaa accaactgag
     1261 agcggcgcgc ggcacccggg cgctcagccc ggcagtggcg ctcacgcgcg cgagcggtcg
     1321 cggacgcggg cttcggcggc cgcgcgatcg ttatcgtcga aggactcgat ggtcacttcc
     1381 atgagggtcg aacgctcgag gcagcgcagg ttgacactgt agccgtcggg gttggagcgc
     1441 gggacgtaga aggatttcac cccgcagcgg cggcagaaca ggtggcgcgc gctgccggta
     1501 ttgaagcgat actcggtgag cgcatccgcg ccgcgcagca gatgcaagcg ggcgcgcggc
     1561 acgatgaggt gcagaaagcc gctcatgcgg cagatcgagc agttgcactc gctcacggtg
     1621 acgcgcgcgg gcgcctcgac ctcgaaggcg accgcgccgc agtgacagcc gccgcggtgc
     1681 ctgatgagct cctcagacaa tgccgccgct ccaggcgggc gccaacagcc gtgcgcggta
     1741 atgccgcagc tcgcgaatcg actcgtggat gtcggcgagc gcgagatggg tgccctgttt
     1801 cacgaagccc tccgccaccg gtggcgccca gcggcgcgcc agctccttga gcgtactcac
     1861 atcgagattg cgatagtgga agaagcgctc gaggtccggc atgtagcggg cgaggaagcg
     1921 ccggtcctgg cagatgctgc tgccgcacat cggtgagctg cccgcgctca ccagtggcgc
     1981 gagaaactcg agtgtacgct gctgcgcctc ggcgacgctg acccggctcg cgcgcacccg
     2041 cgcgagcagc cccgaggagc cgtgctgccg cttgttccac tcgtccatgc gcgcgagcac
     2101 ctcgtcgttg tggtgtatcg cgagcaccgg cccctcggcg aagatcgcca ggtccttatc
     2161 ggtcaccacg gtggcgatct cgatgatgac atcggagtcg ggcttgaggc ccgtcatctc
     2221 cagatcgatc cataccaggt agtcgccgct cggcatgtct atgatgtgcg tcgggtgagc
     2281 atcgtgcgga cgaaagcgtg gcaagtgtag cagaggcggc ggacggacct gagcgatgag
     2341 ccagctcgtg ctcggctccg tcatcgcgac cttcggccgt cacatggtcg tgcgcaccgc
     2401 cgacggcgcg gagttgcggg cgcgtccctt cggacgcacg ctcgaggtgg tgtgcggcga
     2461 tgaggtgcgc tgccgtgtcg atccgcacca cgacgaggtg cacgtcatcg aggtacgccc
     2521 gcggcgcaac gggctgtggc gcgccaactc ccgcggcggc gcggagccgg tggtcgcgaa
     2581 cctcacgcgg ctgctggtag tgctggcgcc cgtgccgctg cccgagctgt tcgtcatcga
     2641 tcgttacctc gccgccgcca ccgccgccgg cg
//
LOCUS       DATDMP010000404         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1732455, whole genome shotgun sequence.
ACCESSION   DATDMP010000404 DATDMP010000000
VERSION     DATDMP010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732455"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VI195_07025"
     CDS             complement(<1..1072)
                     /locus_tag="VI195_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY6824176.1"
                     /translation="MADENADRPVSGNAPRGAPAAPPPLSGATLALGTVALSLATFMN
                     VLDTSIANVSLPAIAGDLGVSPDQGTWVITSFAVANGISVPLTGWLTRRFGQVKLFTS
                     AVVLFVMASILCALSPTLPLLILARVFQGAVAGPMIPLSQALLLASYPKNRAGMALAM
                     WSITTLVAPVVGPVLGGWITDNISWPWIFYINVPVGVAAALATWAIYHKRETPTARVP
                     VDAIGLSLLILWVGAMQIMLDKGKDLDWFNSPMIVTLACVAVVGFAIFIIWELTEEHP
                     VVDLSLFRSRNFTVSTIAMLFAYALFFGNVVLLPLWLQQYMGYTATLAGWVLAPVGFL
                     AIVLSPVVGRINDRVDPRILVTL"
     gene            complement(1100..>2132)
                     /locus_tag="VI195_07030"
     CDS             complement(1100..>2132)
                     /locus_tag="VI195_07030"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY6824177.1"
                     /translation="RLDPADARVALDQAEAQLARTVRDVRNLYATSSQLAAAVQMRQT
                     ELGAAQSDLARRQRLGATGAVSGEELQHSADAVKTAQAELIAAQQQLVANRARVDGTT
                     LQDHPQVRDAAAAVRNAYLTLERTELAAPVSGFVARRNVQLGQRVSPGTALMAVVPLD
                     QVWVDANFKEPQLAHMRIGQHVLLTADLYGGHVSYHGTVAGFGAGTGAAFSLLPAQNA
                     TGNWIKIVQRVPVRIALDPREIAAHPLQIGLSMKADVEVRGAAAGARLPQVAGNQPAW
                     TTAVTRESDTQADARVQAIIAANQSAALPAPAAHALPAGEALPAAGARPASHLVVNVP
                     LPGAARHLH"
BASE COUNT          379 a          708 c          736 g          309 t
ORIGIN      
        1 agagtgttac gaggatgcgg gggtcgacac ggtcgttgat gcggcccacc accgggctca
       61 gcacgatggc gaggaatccg accggggcca gcacccagcc ggcaagcgtc gccgtgtaac
      121 ccatgtactg ctgcagccac agcggcagca ataccacgtt gccgaagaac agggcatagg
      181 cgaacagcat cgcgatcgta ctgaccgtga agttgcggct gcggaacagc gacagatcga
      241 cgacggggtg ctcctcggtg agctcccata tgatgaaaat cgcaaagccc accaccgcca
      301 cgcatgcgag ggtcacgatc atcggcgagt tgaaccagtc gagatccttg cccttgtcca
      361 gcatgatctg catggcgcca acccagagga tcagcagcga gagcccgatg gcatcgaccg
      421 gcacccgagc ggtcggggtc tcgcgcttgt ggtagatggc ccaggtggcg agggcagcgg
      481 ccacaccgac cggcacgttg atgtagaaaa tccagggcca ggagatgttg tcggtgatcc
      541 agcccccgag caccggccca acgaccggcg ccaccagcgt ggtgatcgac cacatggcga
      601 gcgccatgcc ggcgcgattc tttgggtagc tcgcaagcag caatgcctgc gagagcggga
      661 tcatcggccc tgcgaccgca ccctggaaca cccgcgcgag gatcagcagc ggcagggtcg
      721 gcgaaagcgc acacaggatg gaggccatga cgaacagcac gaccgccgag gtgaacagct
      781 tcacctggcc aaagcgccgc gtgagccagc cggtgagcgg aacggagatg ccgttcgcca
      841 ctgcaaagga ggtgatgacc caggtgccct ggtcgggcga gacgccgagg tctccagcaa
      901 tcgcgggcag cgagacgttg gcgatcgacg tgtcgagcac gttcatgaag gtcgccagtg
      961 acagcgcgac ggtaccgagc gcgagcgtcg caccgctcag cggcggtggt gcggcgggcg
     1021 ctccccgcgg agcgttgccg ctcaccggtc tgtcagcatt ttcgtcggcc atgaccttgg
     1081 cgccctcggg ccggaagtct cagtgcagat gtcgagctgc acctggcagc ggcacattga
     1141 caaccaggtg gcttgccggg cgtgctcccg ccgcgggcag cgcttccccg gccggcagcg
     1201 cgtgcgccgc cggtgccgga agcgccgccg actggttggc ggcgatgatc gcctgcacgc
     1261 gggcatccgc ctgggtatcg ctctcacggg tcaccgcggt cgtccaggca ggctgattac
     1321 cggcgacctg cggcagccgc gcaccggcgg cagcgcctct cacctccacg tcagccttca
     1381 tcgacagccc gatctgcagc ggatgagcgg cgatctcacg cgggtcgagc gcaatgcgca
     1441 ccggcacgcg ctgcacgatc ttgatccagt tgccggtggc attctgcgcc ggcagcagcg
     1501 aaaaggcggc gccggtgccc gcaccgaagc ccgcgaccgt gccgtgatag ctcacatggc
     1561 cgccgtagag atctgcggtc agcagcacgt gctgtccgat gcgcatgtgc gcgagctgcg
     1621 gctccttgaa attggcatcc acccagacct gatcgagcgg caccacggcc atgagggcgg
     1681 taccggggct cacgcgctgg ccgagctgca cattgcgccg cgcgacgaag ccggagaccg
     1741 gcgccgcaag ctcggtgcgc tcgagcgtaa ggtaggcatt gcgcaccgcg gccgcggcat
     1801 cgcgcacttg cgggtgatcc tggagcgtcg tgccatcgac ccgcgcgcgg ttggccacga
     1861 gctgctgctg cgccgcgatg agctccgcct gagctgtctt caccgcatcc gcggagtgct
     1921 gcagctcttc tccggaaacg gcaccggtcg cgccaaggcg ttggcgtcga gcgagatcac
     1981 tctgagcggc gccgagctcg gtctgccgca tctgcactgc ggcagcgagc tgcgagctgg
     2041 tggcgtagag gttgcgcaca tcgcgcaccg tgcgggcgag ctgcgcctcg gcctgatcga
     2101 gcgccacacg tgcatcggcc gggtcgagcc gc
//
LOCUS       DATDMP010000405         4995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1065766, whole genome shotgun sequence.
ACCESSION   DATDMP010000405 DATDMP010000000
VERSION     DATDMP010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065766"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VI195_07035"
     CDS             complement(<1..364)
                     /locus_tag="VI195_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6824178.1"
                     /translation="MGYTTSGIRNIALVGQAGAGKTSLVETLLLQAGATRSRGTLARG
                     TTVSDFDPQEKRLQHSLDSTVCSFDFNSTHINLIDTPGYPDFLGKTLSVLEAVEAVAL
                     VVSAASGVDTFTQRMMEFA"
     gene            500..694
                     /locus_tag="VI195_07040"
     CDS             500..694
                     /locus_tag="VI195_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824179.1"
                     /translation="MSDPAQDEARKRHQKIQRQLDEALAATFPASDPVSIVTSHEEED
                     WGEDKTPPEPNPPASPRKGS"
     gene            complement(763..2124)
                     /locus_tag="VI195_07045"
     CDS             complement(763..2124)
                     /locus_tag="VI195_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY6824180.1"
                     /translation="MSDDWRNYVRRIEELAAASGLKFHPVDFEAVPDSFMMEIAVYGL
                     PVRMPHWSFGVRYIYQLIQRHMGHSRLFEVVFPGNPGHAYLADSNGLAENILVTAHVL
                     GHADFSRNNLLFRRCQEQVSERIVEHAANHARQISQAIETHGAQRVEATLDAALALEQ
                     HVDIDQPLRRERYPEYLEDTRTLVDDQFRKRFAALEPAAASSGIEVKKRAPVPPHPER
                     DLLWFVANYAPEMESWERDIFLAVREESFYFYPVFATQIMNEGWASYWHARLMREADF
                     IPQQAYLDAIKCHSDVVRPVAAEQQISLSINPYHLGFSMWEKIVEREGLEAARRIMEQ
                     DDDFGFVRNHLTRELAEELDLFRYSARSDGQIKVLEPELTSLHEAVLAPKYNFGAPIV
                     SAARVRNDGTLELVHDSAVDGRGLDLERSRKVLDYLQRVWRRPIVLTTVNQAGSALEL
                     TAP"
     gene            complement(2121..3329)
                     /locus_tag="VI195_07050"
     CDS             complement(2121..3329)
                     /locus_tag="VI195_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEY6824181.1"
                     /translation="MSTEDPKRADKAGAAKWYELFSRGARDWLRHDEKVREAVRANLP
                     EIIAGGDVINDGARTVRVPVRMLEHYHFRLRRPEEQQGAGQGKAKPGDVFSDPTRSRG
                     PGEKGAGGRDEGEVQLLLEFKIDDIVDWLWEEMQLPNLKARAGPSDETDWTREGWDRR
                     GARSRLDRRRSFKELVKRRGAPGATPTFTDEDLRFRQLARRRQPAVHAVVFFMLDVSG
                     SMSDRDRKLAKTFFFWVVQGLRREYRSLETVFVAHTTEAWEFNEPDFFQVSGTGGTVA
                     SSGLMKVREIIDARYNPGRCNIYLFYASDGDNSVSDAQDARTALGSLVADACFTGYVE
                     VSSGLSRQLATETGKLFAELTTAGCAAGSYALNDFDDVWGAVRHFFTAESHAPEGTRT
                     SEGPAPGGSP"
     gene            complement(3363..>4995)
                     /locus_tag="VI195_07055"
     CDS             complement(3363..>4995)
                     /locus_tag="VI195_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824182.1"
                     /translation="DVGRRLLLLLGPPSGGKSTLAILLKRGLEEYSRTDEGALYAIRG
                     SPLRENPLNLIPTSLRAEFRERYGVNIQGELSPWARDFVEREFEGDFVRVPVERVFLA
                     EASRVGIGTYAPHDPTTADIADLVGSVDLAKVAQIGDEGDPRAWSWSGAVYAASRGVL
                     EMIEILKVKREFLYLLLTLTQEKNVKVSRFPLIHLDETILAHTNLAEFHKFLQEKENE
                     ALLDRMVIIKIPYALSYRDESRIYQKLVSGAPAFRNVHLDPHVLNLAAVFGILTRLTK
                     PEREGLDVPKKLRLYAGEAVEGVPETEAVRLHAESADEGMTGVSPRFVINAISNAITR
                     GNTHSLTSMELLLALKDGIESDARMDVKQKKAWIDHLVTARKEFYNRWVKEDVHRAMF
                     ASFEEEAQQLLEKYLDEVEASLDHRQVSDPITGESRAADERFLRSVEEKIKVSDSGKL
                     SFRQEVVRKAMVAFKAGEKFKLASHARMREAIEQYLFEERRDVLRLVTSTARPDEEAR
                     QKISVVQQRLVNEYGYDEHSAKEALSYVTTLLAQE"
BASE COUNT          842 a         1633 c         1643 g          877 t
ORIGIN      
        1 gtgcgaactc catcatgcgc tgcgtgaagg tatctacgcc gctcgccgcg ctcaccacca
       61 gcgcgaccgc ctccaccgct tccagcaccg acagagtctt accgaggaag tccggataac
      121 ccggggtgtc gatgaggttg atgtgggtgc tgttgaaatc gaagctgcag acggtgctgt
      181 cgagcgagtg ctgcaggcgc ttctcctgcg gatcgaagtc cgagaccgtc gtaccccgtg
      241 ccagggtgcc gcggctgcgc gtggcgccgg cctgcagcaa caatgtttcc acgagcgagg
      301 tcttacccgc gccggcctga ccgaccagcg cgatgttgcg gatgcccgaa gtcgtatacc
      361 ccatggtggc tggtcccccg gcaaaagacc ggcggcgcgc ggggcatcac cggctctgaa
      421 agcctactcc gcctgacctg ccgtaggcta cttgagggcg taccatgtgt ccgaggccca
      481 gagcttgcag gagtgcgtaa tgagtgaccc ggcccaggat gaggcgcgca agcggcacca
      541 gaagatccag cgtcagctcg atgaggcgct ggcagccact ttcccggcga gcgacccggt
      601 atcgatagtg acctcgcacg aggaggagga ctggggcgag gacaagacgc cgcctgagcc
      661 gaacccgccc gcttctccgc gcaagggaag ctgagcggac ccgccgcccg cagcccaaag
      721 gggaggttaa cccgggggct gcccgacgcc ccgtgccggg tgctacggcg ccgtgagctc
      781 cagggcggat cccgcctgat tgaccgtggt cagcacgatc gggcggcgcc agacgcgttg
      841 caggtaatcg agcaccttgc ggctgcgttc gaggtcgaga ccccgcccgt cgacggcgct
      901 gtcgtgcacg agctccagcg taccatcgtt acgcacgcgc gccgccgaga cgataggcgc
      961 gccaaagttg tatttcggtg ccagcaccgc ctcgtggagc gaggtgagct cgggctcgag
     1021 caccttgatc tggccgtcgc tacgcgcgct gtagcggaac agatcgagct cctcggcgag
     1081 ctcgcgcgtc aggtgattgc gcacgaagcc gaagtcatcg tcttgctcca tgatgcggcg
     1141 cgcggcctcg agcccctcgc gttccacgat cttctcccac atcgagaacc cgagatgata
     1201 cgggttgatg ctgagtgaga tctgctgctc cgcggccacc gggcgcacga cgtccgagtg
     1261 acacttgatg gcgtcgaggt atgcctgttg gggtatgaaa tccgcctcac gcatcagccg
     1321 ggcgtgccag tacgaggccc agccctcgtt catgatctgc gtggcgaaca ccggatagaa
     1381 atagaacgac tcctcgcgca ctgcgagaaa gatgtcgcgc tcccagctct ccatctccgg
     1441 cgcgtagttc gcgacgaacc acagcaggtc gcgctccggg tgcggcggga ccggcgcgcg
     1501 cttcttgact tcgatgccgc tgctggctgc ggcgggctcg agcgcggcga agcgcttgcg
     1561 gaactgatcg tccaccagcg tgcgggtgtc ctcgagatat tccgggtagc gctcgcgccg
     1621 cagcggctga tcgatgtcga cgtgctgctc cagggcgagc gcggcatcga gggtggcttc
     1681 gacccgctgc gcaccgtgcg tctcgatcgc ctgcgagatc tggcgggcat gattggcggc
     1741 atgctcgacg atgcgctcgc tcacctgctc ctggcagcgg cggaacagca gattgttgcg
     1801 cgaaaaatcc gcgtgcccga ggacgtgcgc cgtcaccagg atgttctcgg cgagcccatt
     1861 gctgtcggcg aggtaggcgt gcccgggatt gccgggaaac accacttcga acaagcgcga
     1921 gtggcccatg tgacgctgga tgagctggta gatgtagcgc acgccgaagg accagtgcgg
     1981 catgcgcacc ggcagcccgt acaccgcgat ctccatcatg aagctgtcgg gcaccgcctc
     2041 gaaatcgacc ggatggaact tcagtccgga agcggccgcc agctcctcga tgcggcgcac
     2101 gtagttgcgc cagtcatcgc tcatgggctg ccgccgggcg cgggaccctc gctggtccgc
     2161 gtgccctccg gcgcgtggct ctcggcggta aagaagtgcc gcaccgcgcc ccagacgtca
     2221 tcgaagtcat tcagtgcata gctgccggcg gcacacccgg cggtggtgag ctcggcgaag
     2281 agcttgccgg tctcggtggc gagctggcgc gacagtcccg aggaaacctc gacatagccg
     2341 gtgaagcagg cgtcggcaac gagcgagccg agcgcggtgc gtgcgtcctg cgcgtcactc
     2401 acggaattgt cgccgtcgga ggcgtagaag agatagatgt tgcagcggcc ggggttgtag
     2461 cgcgcatcga tgatttcacg caccttcatc agccccgatg aggccaccgt accgccggtg
     2521 ccgctcacct ggaagaagtc cggctcgttg aattcccatg cctcggtggt gtgcgcgacg
     2581 aacaccgtct cgagcgagcg atactcgcgc cgcaggccct gcaccaccca gaagaaaaaa
     2641 gtcttggcga gtttgcggtc gcggtcggac atgctgccgg agacatcgag catgaagaac
     2701 accacggcgt gcacggccgg ctgccgccgc cgcgcgagtt gccggaagcg caggtcctcg
     2761 tccgtgaagg tgggtgtggc gccgggagcg ccgcgccgct tcaccagttc cttgaaggag
     2821 cggcggcggt cgagccgcga gcgcgcgccg cggcggtccc agccttcgcg tgtccaatcg
     2881 gtttcatcgg agggcccggc gcgggccttg agattcggca gctgcatttc ctcccacagc
     2941 cagtcgacga tgtcgtcgat cttgaactcg agcagcagct gcacctcgcc ctcgtcgcga
     3001 ccgccggcac ctttctcacc agggccgcga ctgcgggtgg gatcggagaa tacgtcgccc
     3061 ggcttcgcct tgccctggcc cgcgccctgt tgctcctcgg gccgccgcag gcggaagtgg
     3121 taatgctcga gcatgcgcac cggcacgcgc accgtgcggg cgccatcgtt gatcacatct
     3181 ccgccggcaa tgatctccgg cagattcgca cgcaccgctt cgcgcacctt ctcatcgtgg
     3241 cgcagccaat cgcgcgcgcc gcgcgagaac aactcgtacc atttggccgc accggccttg
     3301 tccgcgcgct ttgggtcctc ggtgctcacg gcactagtgg tacacctttt ccgggcgcgc
     3361 cgctactcct gagcgagcag tgtcgtgaca tagctgaggg cttccttggc gctgtgctca
     3421 tcgtagccat actcgttcac caagcgctgc tgcacgaccg agatcttctg ccgtgcctcc
     3481 tcgtctgggc gtgcggtcga ggttaccaga cgcagcacat cgcgccgctc ctcgaacagg
     3541 tactgctcga tcgcctcgcg catgcgtgcg tggctcgcca gcttgaattt ctccccggcc
     3601 ttgaaggcca ccatagcctt gcgcacaact tcctggcgga acgagagctt gccggaatcc
     3661 gacaccttga tcttctcctc gaccgagcgc aggaagcgct catccgcggc acgactctcg
     3721 ccggtgatcg ggtcgctgac ctggcggtga tcgagcgacg cctcgacctc gtccagatac
     3781 ttctccagca actgctgcgc ttcctcttcg aaggaagcaa acatggcgcg gtgcacgtcc
     3841 tcctttaccc agcggttgta gaactccttg cgcgcggtca ccagatgatc gatccaggct
     3901 ttcttctgct tgacgtccat gcgggcatcg ctctcgatac cgtccttgag cgcgagcagc
     3961 agctccatgc tggtgagact gtgggtattg ccgcgggtga tggcgttgga aatggcattg
     4021 atgacgaagc gcgggctcac accggtcatg ccttcatcgg ctgactcggc atgcagtcgc
     4081 accgcttcgg tctccggcac gccttcgacc gcttcgccgg cgtacaggcg cagctttttc
     4141 ggcacgtcga gcccctcgcg ctcgggcttg gtgaggcgcg taaggatgcc gaacactgcc
     4201 gcgaggttga gcacgtgcgg gtcgaggtgc acgttgcgga acgcgggcgc accggacacg
     4261 agcttctggt agatgcgcga ctcatcgcga taggagagcg cataggggat cttgatgatc
     4321 accatgcggt cgagcagcgc ctcgttctcc ttctcctgca ggaacttgtg gaactcggcg
     4381 agattggtgt gcgcgagtat ggtctcgtcc aggtggatga gcgggaagcg cgataccttg
     4441 acgttcttct cctgcgtcag tgtcaacagc aggtagagga actcgcgctt gaccttgagg
     4501 atctcgatca tctcgagcac gccgcggctc gcggcataca ctgcaccgga ccacgaccag
     4561 gcgcgcggat cgccctcatc gccgatctgc gcgactttgg cgagatcgac cgagccgacc
     4621 aggtcggcga tatcggcggt ggtggggtcg tgcggcgcgt aggtgccgat gccgacgcgc
     4681 gaagcctcgg cgaggaagac acgctcgacc ggcacgcgca cgaaatcacc ctcgaactcg
     4741 cgctcgacga aatcgcgcgc ccagggcgag agctcaccct ggatgttgac gccataacgc
     4801 tcgcgaaact cggcgcgcag gctggtcgga atcaggttga gcgggttctc gcgcagcggc
     4861 gagccgcgga tggcgtacaa cgcaccttcg tcggtgcggc tgtattcctc caggccgcgc
     4921 ttgagcagaa tggccagggt cgatttgccg cccgagggcg gtcccagcag cagcagcagg
     4981 cggcggccga catcc
//
LOCUS       DATDMP010000406         5159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1732720, whole genome shotgun sequence.
ACCESSION   DATDMP010000406 DATDMP010000000
VERSION     DATDMP010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732720"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VI195_07060"
     CDS             complement(<1..714)
                     /locus_tag="VI195_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824183.1"
                     /translation="MNFSRQFLALLRMSLAATAQRLGSVVTIIIGDTCAVAVLVSMLA
                     MGSGARRQVLAGVHEDRVVVNSHGASGIQSSIPRDEADTVRDLPGIKKGSDGKPLVEF
                     QSIVPIEARRRVTGRRIFFPLVGITGNPVASDPELHLTEGRVFRPGLHELIASRPCAR
                     QFTGFGIGDKRPIGGADWTIVGHFDQGDSQQCVVLADAEILMSTFKSNAYTQATATLQ
                     SPADYPMLRSALESNPALHL"
     gene            complement(711..1865)
                     /locus_tag="VI195_07065"
     CDS             complement(711..1865)
                     /locus_tag="VI195_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824184.1"
                     /translation="MKYLPLIWSGLWRKPGRTILVFLQVSVAFALFGVLQGLKTGVAH
                     AVAAARADLLLVHSRLSLIMQPLPYGLLEQIRTVPGVKLAIPVELTSGTYQRPDQGVG
                     VVAVGPEPDWPSAFTYTIAPEYLAAFRKSRTAMLVKDLTAQKYGWKVGARIPLKLPVA
                     QQNGSTDWAFDMVGTFSDSDIGGGRYIVIVSYPYLDEARLTGKGTLNHFNVAVTDPRL
                     AAQVADAIDARFANSSHETKTESLREMAQASMQAIGDFDFLIRAVVGAVLVALLFATT
                     TMMVQSIHERIPELAVIKTLGFSDRAVFLLVLAEALVAFLTGAACGLVLATLALPIAA
                     RFVVGLSMPGVVVVTGLALGALVALISAMVPAALAARLRVATALAGHRAA"
     gene            1973..2902
                     /locus_tag="VI195_07070"
     CDS             1973..2902
                     /locus_tag="VI195_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY6824185.1"
                     /translation="MTKRTAFALLAAMLPALYDTAQAATLGVSMASFDDNFLTAVRAS
                     MKARAQQLNAGIQFEDAQSDIGRQLNQIENFIAQKVDAIIVNPVDADATPRMSRLATR
                     AAIPLVYVNRLPGDKQLPPRVAFVGSDETQSGTLQMQEVCRLLQGKGDIVIMMGELTN
                     QSTRQRTQDVYDVIKRPQCLGIRVLAKQAANWKRTEAADLMTNWLSAGLRPQAVVAND
                     DEMAIGAIQSLKQARLLSATVVAGIDATADGLAAMKAGDLKVTVFQNAAGQGSGAVDT
                     ALKLAHGEKVASFVWVPFELVTPATLATYLTRH"
     gene            2908..4419
                     /locus_tag="VI195_07075"
     CDS             2908..4419
                     /locus_tag="VI195_07075"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY6824186.1"
                     /translation="MHAAAAHAPDRCLLEVRHISKSFPGVAALDDVSLRVDRGTVHAL
                     IGENGAGKSTLMKIIAGIVPANAGEVRLAGRTLRLDTPRQALESGIAMIHQELNLMPT
                     MSVAENIWIGREPVNAFGMVNHRELHRRTRALLERLQIDVDPQAEVRALPIASRQLVE
                     IAKALSYESQLLIMDEPTSALSEHDTVQLFTLIRELKPQGKAVLYITHRMDELREIAD
                     EVTVLRDGRYIGSERPAKLTREQIIRMMVGRELAQTSPRAPAMPGAVRLAVRALTLQP
                     HFHDVSFELHAGEILGVAGLIGAGRSKLAEALFGVTPASSGQIRIDGAEVRVDSPCTA
                     LARGMAFLTEDRKETGCLLTLDVLENLEVAVLNRDFVRFGFLRRAAVMQACADMSQRL
                     RVKTPSLREPIAHLSGGNQQKVLIGRWLLTAPRILILDEPTRGIDVGAKAEIHQLIAR
                     LAAQGAAVLMISSELPEILGMSDRIMVMHAGGVAGVLERAQADQEKIMALAAH"
     gene            4439..>5159
                     /locus_tag="VI195_07080"
     CDS             4439..>5159
                     /locus_tag="VI195_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824187.1"
                     /translation="MPEALMQQSIPSPAARRTRHWPPEASILLVLVGLALLFELLGWV
                     FVRQSFLLNEQRLVVMILQVSVIGIIAIGVTQVIITGGIDLSSGSVVALTAMVAASLA
                     QSADAVHPVYPSLIELPVIVPVAAGLAVGAAAGLLNGSLIARTSIPPFIATLGVMVSA
                     RGLAKWYTRGQPVSLLTDAYANVGNGAVPVVIFLATALIFHIALRYTRYGKFTYAIGA
                     NVQAARVSGINIGRHLIKVYAI"
BASE COUNT          918 a         1726 c         1671 g          844 t
ORIGIN      
        1 gaggtgcagc gccggattgc tctcgagtgc gctgcgcagc atcgggtagt ccgccggcga
       61 ctgcagcgtc gccgtggcct gagtgtaggc attgcttttg aacgtcgaca tgaggatctc
      121 ggcgtccgcg agcaccacgc attgctgtga gtctccctga tcgaaatgcc cgacgatcgt
      181 ccagtcggca ccgccgatcg gacgtttatc gccaatcccg aatccggtga actggcgcgc
      241 gcatgggcga ctggcaatga gctcgtgcag accgggccgg aatacacgtc cttcggtaag
      301 gtgcagttcg gggtccgacg caaccgggtt gccggttatt ccaaccaagg ggaagaagat
      361 ccgcctgccc gtcactcgac gacgagcttc gatcggcacg atcgactgaa actcgaccaa
      421 cggtttaccg tcgctgccct tcttgattcc cggcaggtca cgcacggtgt cggcctcatc
      481 gcgggggata ctgctctgga tgccgctcgc gccgtggctg ttcaccacca cccggtcctc
      541 gtggaccccg gccagcactt gccggcgggc gccgcttccc atggccagca tcgagaccag
      601 taccgccacc gcacaggtgt cgccgatgat gatcgtgacg accgagccca gacgctgcgc
      661 cgtcgctgcc aggctcatgc gcagcagcgc cagaaattgc cggctgaagt tcatgcggcc
      721 cggtggccag ctagtgcggt ggcgacccgc agccgtgccg cgagggccgc tgggaccatc
      781 gcactgatga gcgccaccag tgcgcccaat gccaggcccg taaccaccac cacacccggc
      841 atcgacagcc cgactacgaa acgcgccgca atgggtagcg cgagcgtcgc cagcacgagc
      901 ccgcatgcgg caccggtgag aaacgcgacc agcgcctcgg cgagcacgag cagaaatacg
      961 gcacgatccg aaaatcccaa ggtcttgatc acggcgagct ccgggatacg ctcgtgtatc
     1021 gactggacca tcatggtcgt ggtcgcaaac aacagcgcga ccagcaccgc acctaccacc
     1081 gcacggatca ggaagtcgaa gtcgccgatc gcctgcatcg aggcctgcgc catttcccgc
     1141 agggattcgg ttttggtctc gtgggaggaa ttggcgaaac gcgcatcgat cgcatcggcc
     1201 acctgcgccg caagcctcgg atcggtgacc gcgacgttga agtgattgag ggtgcccttg
     1261 ccggtgagcc gcgcctcatc cagatatgga tagctcacga tcacgatgta gcgtccgcca
     1321 ccgatgtcac tgtcggagaa ggtgccgacc atatcgaacg cccagtcggt cgagccgttc
     1381 tgttgcgcaa cgggcagctt cagaggaatg cgagcaccga ccttccagcc atacttctgc
     1441 gcggtcagat ccttcaccag catcgccgta cgtgatttgc ggaaagcggc caggtactca
     1501 ggcgcgattg tgtaggtgaa ggcgctcggc cagtccggct ccgggccgac ggccacgacg
     1561 ccgacgccct ggtcgggtct ctgataggta ccggaagtga gctccacggg aatggccagc
     1621 ttcactcccg gaacggtacg gatctgctcg agcagaccgt acggaagcgg ttgcatgatg
     1681 aggctcaggc ggctgtgcac gaggagcaga tcggcgcgtg cggcggcgac ggcgtgtgcc
     1741 acgccggttt tcagaccctg cagcacgccg aacagcgcaa aggccacgct gacctggagg
     1801 aagacaagga tggtgcgacc cggcttgcgc cacaggcccg accagatgag cggcaggtac
     1861 ttcaccgcac gctacgctcc tgttgttgcg cctacggcgt acgaacacta acaaagctca
     1921 tcgcaggcgt catccagtcg catctgcgtg gcccggcgct tagactgcag cgatgacgaa
     1981 gcgcaccgcc ttcgcactcc ttgccgcgat gctgccggcg ctgtacgaca cagcgcaggc
     2041 ggcgactctg ggcgtgtcga tggcgtcgtt cgatgataat tttctcaccg cggtgcgcgc
     2101 cagcatgaaa gcgcgggcgc agcagctcaa tgccggcatt cagttcgagg acgcccagag
     2161 cgacatcggg cgtcaactga atcagatcga gaatttcatc gcgcaaaagg tggacgcaat
     2221 catcgtcaac cccgtggacg ccgatgcgac gccgcgcatg agtcggctcg ccacgcgcgc
     2281 cgccatcccg ctcgtgtacg tgaaccgcct gcccggggat aaacaactgc cgccccgcgt
     2341 ggccttcgtc ggctccgatg agacccagtc gggcaccttg cagatgcagg aggtgtgccg
     2401 gctgctgcag ggcaaaggcg acatcgtgat catgatgggc gagttgacca accaatcgac
     2461 ccgccagcgt actcaggacg tctatgacgt gatcaagcgg ccgcagtgcc tgggcatccg
     2521 cgtgctcgcc aagcaggcgg ccaactggaa gcgcaccgag gccgccgacc tcatgaccaa
     2581 ctggctgagc gccggcctgc gcccgcaggc ggtggtcgcc aacgatgacg agatggccat
     2641 cggtgcgatt cagtcgctga aacaggcgcg cctgctgtcc gccaccgtag tggcgggaat
     2701 cgatgccacc gcggatgggc tcgcggcgat gaaagcgggc gatctcaagg tgacggtgtt
     2761 tcagaatgcc gccggccagg gcagcggcgc cgtcgacacg gcgctgaagc ttgcccacgg
     2821 cgagaaggtc gcgagcttcg tatgggtacc gttcgagctc gtgacaccgg cgacgctcgc
     2881 gacctatctg acccggcact gaactgcatg cacgccgcgg ccgcgcacgc gcccgatcgc
     2941 tgtttgctcg aggtgcgcca catcagcaag agcttcccgg gagtggcggc gctcgatgat
     3001 gtatccctgc gggtcgaccg gggaacggtg cacgcgctca tcggtgagaa cggcgccggc
     3061 aaatccaccc tcatgaaaat catcgccggg atcgtcccgg cgaatgcggg cgaggtacgt
     3121 ctcgctgggc gcacgctgcg actcgacact ccgcgccagg cgctcgagag cggcatcgcc
     3181 atgatccatc aggagctcaa cctgatgccc accatgagcg tcgcggagaa catctggatc
     3241 ggccgggagc ccgtcaatgc tttcggcatg gtcaaccacc gcgagctcca tcgccgcacg
     3301 cgcgcgctgc tcgagcgcct gcagatcgat gtggacccgc aggccgaagt gcgcgcgctg
     3361 ccgatcgcga gccgccagct ggtggagatc gccaaggccc tctcgtacga atcgcagctc
     3421 ctcatcatgg atgagcccac ttccgcgctc agcgagcacg acacggtgca actgttcacc
     3481 ctcatccggg agctgaagcc gcagggcaag gcggtgctgt acatcacgca caggatggat
     3541 gagctgcgcg agatcgcgga tgaggttacg gtgctgcgcg acggccgcta catcggcagc
     3601 gagcgccccg ccaagctgac gcgcgagcag atcattcgca tgatggtcgg acgcgagctc
     3661 gcacagacat ccccgcgcgc gccggccatg ccgggcgccg tgcgcctcgc cgtgcgggcg
     3721 ctcacgctcc agcctcactt ccacgacgtc agcttcgagc tgcacgcagg cgagatcctc
     3781 ggtgtcgcgg gtctcatcgg cgccggccgc agcaagctcg ccgaggctct cttcggcgtg
     3841 acgcctgcga gcagcggcca gatccggatc gatggcgccg aagtcagggt cgattcgcct
     3901 tgcaccgcac tggcgcgcgg catggcgttc ctcaccgagg accgcaagga gaccggctgc
     3961 ctgctcacgc tcgatgtgct cgagaacctc gaggtcgccg tgctcaatcg cgacttcgtg
     4021 cgtttcggct tcttgcgccg cgccgcagtc atgcaggcct gcgccgacat gagccagcgg
     4081 ctgcgcgtga agacaccgag cctgcgggag ccgatcgcgc atctctcggg cggcaatcag
     4141 cagaaggtgc tgatcggccg ctggctgctc accgctccga ggattctcat cctggacgag
     4201 ccgacccgcg gcatcgatgt gggggccaag gccgagatac accagctcat cgcgcggctc
     4261 gccgcgcagg gagctgcagt gctcatgatt tcctcggagc tgcccgagat cctcggcatg
     4321 agcgaccgca tcatggtgat gcacgcaggc ggcgtcgccg gcgtgctcga gcgcgcacag
     4381 gcggatcagg agaagatcat ggcgctcgcg gcccactagc gtgccaagcg gagagcccat
     4441 gccggaggcc ctcatgcagc agagcattcc ctcgcccgcc gcacggcgca cgcggcactg
     4501 gccgcccgag gccagcatcc tgctggtgct ggtcggcctc gcgctgctct tcgagctgct
     4561 cggctgggtg ttcgtgcggc agagcttcct gctgaatgag cagcgcctgg tggtcatgat
     4621 cctgcaggta tcggtgatcg gcatcatcgc catcggagtg acgcaggtga tcatcaccgg
     4681 cggcatcgac ctttcttcgg gctcggtggt ggcgctcacg gcgatggtgg cggcgagcct
     4741 cgcgcagtcc gcggacgccg tgcatcccgt gtacccctcg ctcatcgagc tgccggtgat
     4801 cgtgccggtc gctgccggtc tcgccgtggg cgccgcggcc gggctgctga acggctccct
     4861 catcgcccgc acttccatcc cacccttcat cgccacgctc ggggtgatgg tgagcgcgcg
     4921 cgggctcgcc aagtggtaca cgcgcggaca gccggtgagc ctgctcaccg acgcctatgc
     4981 caacgtcggc aacggtgcgg taccggtcgt catatttctc gcgaccgcgc tgatctttca
     5041 catcgcgctg cgctacacgc gctatggcaa gttcacctat gcgatcggcg ccaatgtgca
     5101 ggcggcgcgg gtctccggca tcaacatcgg ccggcacctg atcaaggtct atgccatcg
//
LOCUS       DATDMP010000407         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_733276, whole genome shotgun sequence.
ACCESSION   DATDMP010000407 DATDMP010000000
VERSION     DATDMP010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733276"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VI195_07085"
     CDS             complement(<1..230)
                     /locus_tag="VI195_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824188.1"
                     /translation="MQRPVSIYRELLLAAWMVWFAYWVVSALRVKQTERRESAGSRLA
                     YLLPLIVGAWLIALPHVRLGPLSVQLWPDEAAR"
     gene            318..1208
                     /locus_tag="VI195_07090"
     CDS             318..1208
                     /locus_tag="VI195_07090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY6824189.1"
                     /translation="MSQSPLSAQLDAERSGILNRVAASVLAVETQRTRQSAFLWRGGL
                     LVTAAGPLEGADSARSLRDGSASDAEVLACDLRTDIAVLRVEQPLGPALAAAATLPRP
                     GQLIVVAGRARAELSASFGSVQLSAGPWTTRRGATLSRRLLLDVRLAPDAEGAPVLDL
                     EGGVLAMAVRGPVNQVIGIPADALEAAVSDVATHGRIAQGYLGVRVVPLALGLQARHR
                     LDAGRAVLIVADVAQPSPAFDAGLEIGDLLLAVDGEVVQAPDRLLGRIQGKRPGEALL
                     LRRRRGAAIDELTVTLGSRS"
     gene            1236..1847
                     /locus_tag="VI195_07095"
     CDS             1236..1847
                     /locus_tag="VI195_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6824190.1"
                     /translation="MVQIIVAAYQVEVRAALQSRLEAGGLTAASEIDAPAAFGRALAE
                     LTEPLLVAGVRWPETAPFLERASAHDIPIALLLEEESLDAGHPAFRRGAAALLPWPTE
                     VSQLSSALEAIGYGLRVLPGALPGARSARRAALAPRTMLSPRERALLELVAAGFGNKA
                     IARRLGVSINTVKYHLASIFAKLAARTRAEAVSAAARSGELML"
     gene            complement(1976..>2267)
                     /locus_tag="VI195_07100"
     CDS             complement(1976..>2267)
                     /locus_tag="VI195_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6824191.1"
                     /translation="EVRIDFAWGGHVDITLNRAPHFGRLAPNIYFLQGFSGHGIALTG
                     IAGKLVAQAIGGTASRFDVFARIPHGSFPGGAALRRPALVLAMLWYRLRDLL"
BASE COUNT          349 a          770 c          792 g          356 t
ORIGIN      
        1 cgcgccgcct catccggcca cagctgcacg ctcagcgggc cgaggcgcac gtgcggcagc
       61 gcgatgagcc aggcgccgac gatgagtggc agcaggtagg cgagacgcga gcctgccgat
      121 tcacggcgct cggtctgctt cacacgcagc gccgagacta cccagtaggc gaaccacacc
      181 atccaggccg cgagaagcag ttcccgatag atggagactg gtcgctgcat gcgttctttc
      241 ctcgctaccc atccgggcgg tagcccgccc cggcccgcta cggaacactt cgctcgtacc
      301 tcgggcggag tcataccatg agtcagtcac ctctttctgc acagctcgac gccgagcgca
      361 gcggcatact caatcgcgtc gccgccagcg tgctcgccgt cgaaacgcag cgcacacggc
      421 agtcggcgtt cctgtggcgc ggcgggctgc tcgtcaccgc agccggaccg ctcgaaggtg
      481 ccgactcggc acgcagtctc agggacggct ccgcgagtga cgccgaagtg ctcgcctgcg
      541 atctgcgcac cgacatcgcg gtgctgcgcg tcgagcagcc gctcggcccg gcacttgctg
      601 cggcggcaac cctgccgcgg ccgggtcagc tcatcgtggt ggccgggcgt gctcgagcgg
      661 agctcagcgc gagctttgga tcggtgcagc tgagtgcggg accatggact acgcgacgcg
      721 gcgcgacgct ctcgcgtcgg ctattgctcg atgtgcgcct ggcgcccgac gcagaggggg
      781 ctccggttct cgacctcgag ggtggcgtgc tcgcgatggc ggtgcgcggc ccggtcaacc
      841 aggtgatcgg catacccgcc gacgcgctcg aagctgcggt cagcgatgtc gccacgcacg
      901 ggcgcatcgc tcagggctat ctcggagtac gcgtcgtgcc gctcgcgctc ggcttgcagg
      961 cacggcaccg actcgatgcc ggccgtgcgg tgctgatcgt cgcggatgtc gcgcagccct
     1021 cgcccgcctt cgatgcgggg ctcgagatcg gcgacctgtt gctggcggtc gatggcgagg
     1081 tcgtgcaggc gcccgatcga ttgctgggac gcatccaggg caagcggccc ggtgaggccc
     1141 tgctgctcag gcgcaggcgc ggtgcggcca tcgatgagct cacggtgact ttgggttccc
     1201 gatcttgatt tgcttgcggc gcaccctaca cacggatggt gcagatcatc gtggcggcct
     1261 atcaggtgga ggtgcgcgcg gcgctgcaga gtcgactgga ggcgggcggg ctcaccgcgg
     1321 cgagcgagat cgacgcaccg gctgcattcg gccgggcgct ggcggagctg acagagccgt
     1381 tgctggtcgc gggggtgcgc tggccggaaa ccgcgccatt cctcgagcgc gccagcgcgc
     1441 acgacatccc gatagcgctg ctgctcgaag aggaatcgct cgatgccggg caccccgcgt
     1501 ttcggcgcgg agccgcggcg ctgctgccct ggccaactga ggtctcgcag ctgagctctg
     1561 cgctcgaggc gattggctac ggcctgcgtg tgctgcccgg ggcgctgccc ggggcgcgat
     1621 cggcgcggcg cgcggccctc gctccgcgta ccatgctctc gccgcgcgag cgtgcgctgc
     1681 tggagctggt cgcggccggc ttcggcaaca aggccatcgc ccgaaggctc ggggtctcga
     1741 tcaacacggt caaataccac ctcgcctcga tctttgcgaa gctcgctgcc cgcacgcgcg
     1801 ccgaagcggt gtccgctgcc gcgcgatcgg gcgagctcat gctgtaacac caggcccgca
     1861 ggccgcgctc attcgttcat tgagaggacg cgcggttgag acacgtcttg tgaatggtta
     1921 accaaatggt taaccatcgt cggtcacagc cacctgcaga gcctggcgcg ctccactaca
     1981 gcagatcccg cagccggtac cagagcatcg ccagcacgag cgcagggcgt cgcaatgccg
     2041 cgcctcccgg gaaactgccg tgggggatgc gcgcaaacac atcgaaacgc gaggcggtgc
     2101 cgccgatcgc ttgcgccacc agcttcccgg caatgccggt cagggcaatg ccgtgcccgg
     2161 agaaaccctg gaggaaatag atgttgggag cgagacggcc gaaatgcggt gcccggttga
     2221 gggtaatgtc gacatgacct ccccaggcga agtcgatgcg cacctcg
//
LOCUS       DATDMP010000408         4288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2177831, whole genome shotgun sequence.
ACCESSION   DATDMP010000408 DATDMP010000000
VERSION     DATDMP010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177831"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VI195_07105"
     CDS             <1..289
                     /locus_tag="VI195_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY6824192.1"
                     /translation="ESWILVGTMFTFHYASMFYRSVDDPPLRFPETARPIFWDFIYFS
                     FTIAAACQTSDVTTTRVSIRRVVTAQTLISFLFNVSILGFAINVSAGLLGG"
     gene            347..1759
                     /locus_tag="VI195_07110"
     CDS             347..1759
                     /locus_tag="VI195_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6824193.1"
                     /translation="MNDLQAAVPADQNALSRSLRPRHVAMITIGGIIGAGLFVGSSVA
                     IAAAGPAIIISYVLTGLLILLVMRMLGEMAVEMPQVRSFTEFTRAALGHWAGFSIGWL
                     YWYFWVIVIPVEAIAGAGIIQRWLPLPAWQIGAVLMALMTAVNLMSARAYGEFEFWFS
                     SIKVAAIITFIIAVGAYACGYHSPTGPTFSNLTAYGGFAPRGALAVLASVTTVIWSMM
                     GAEVVTIAAAESPEPARAVARMTSTIISRIVLFYVGSIFVITCVVPWTKVVPGQSPFT
                     LALDQIHFPYASTIMAAVILTAVLSCLNSAFYVASRVLFVLASRGDAPHWLVRTNRRH
                     VPARAVLLASVLGFAGVIAAIVSPSGVFRFLVNSSGAIIAVIYLAIAVSQVRTRSARE
                     AAGAPPPVLPMWLFPWLSYLAIAGVVAVLIAMAVTPSHRAEFWTSVISMVVALLAFVV
                     FRLTAVRPWRGAPQRQPATD"
     gene            1972..3009
                     /locus_tag="VI195_07115"
     CDS             1972..3009
                     /locus_tag="VI195_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY6824194.1"
                     /translation="MRATLMYRAGDVRIENVPDPVLVEPTDALITIHRACICGSDLWP
                     YKELQPVERGRRMGHEAVGVVEAIGTEVRRVKRGDVVVMPFAYSDGTCTFCEAGLQTS
                     CLRGGFFGVGAEAAGAQAEALRVPQADGTLFALAVGRDERLMPSLLSLSDVMGTGHHA
                     AVSAKVRRGATVAVIGDGAVGLCAVIAAKRLGAEQIILMGRHPQRIALAREFGATDIA
                     RERGSEVLERVRQLTQGLGAHSVLECVGSAQAMETALAIVRPGGAIGRVGVPHYSSIT
                     AGGPMFYNNVSVSGGPAPVRAYIEELLPDILEGRIEPGRVFDRVIGLDEVPAGYRAMD
                     ERQALKIMINP"
     gene            3036..3731
                     /locus_tag="VI195_07120"
     CDS             3036..3731
                     /locus_tag="VI195_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6824195.1"
                     /translation="MSRILITGANRGLGLEFAQQYLADGWEVIAACRNPGAAQALRRL
                     ADSATGSLTVIAMDVADRGSVQRAATSRQVAAIDFVINSAGVFGGNGQRLGSLDYRDW
                     MQVLEANLLGPARVCEAFLERIGRSERRLIVTITSGMGSLSDNTSGGSVLYRTSKAAV
                     NMLMRTAAIDLQPQGISCVVLNPGWVKTDMGGPNAKISAAESVGAMRRVISRLGRKDS
                     GRFYNYDGREYPW"
     gene            complement(3757..>4288)
                     /locus_tag="VI195_07125"
     CDS             complement(3757..>4288)
                     /locus_tag="VI195_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6824196.1"
                     /translation="NVKDFGYGDLRDILSGLDAVERAAPIDAARVGLCGWSYGGYMTM
                     WALTQTDRFKAAVSGAGISNWLSYYGTNNIDTWMIPYFGASVYDDPKVYERSSPLTFI
                     KNVRTPTLLVGGDRDAEVPITQSYEYWNALRRLGVKTEFVVYPDEGHHFFKRSDQIDL
                     MERIVGWFNSYLKSTP"
BASE COUNT          701 a         1358 c         1406 g          823 t
ORIGIN      
        1 ggagtcctgg atactcgtcg ggaccatgtt cactttccat tacgcgagca tgttctatcg
       61 ctcggtggat gatccaccgc tgcgttttcc ggagaccgcg cgtcccatat tctgggattt
      121 catctacttc tcgttcacca tcgccgcagc ctgtcagacc tccgatgtca ccactacccg
      181 ggtatcaatc cgccgcgtag tcaccgcgca gacgctgatc tccttcctct tcaacgtgtc
      241 catcctgggc ttcgccatca atgtgtcggc cgggctgctc ggcggttaaa tccgatcggg
      301 tgcacggcgc gcacccaggc ggagcgatgg gtatactcgg caggaaatga acgacctcca
      361 agccgcagtg ccggccgatc agaacgcgct cagccgctct ttgcggccac ggcatgtcgc
      421 gatgattacc atcggcggca tcatcggcgc cgggctgttc gtaggcagca gcgtggccat
      481 cgccgctgcc gggcctgcga tcatcatcag ctacgtgctc accgggctgc tgatactgct
      541 ggtgatgcgc atgctggggg aaatggcggt cgagatgccg caggtccgct cgttcaccga
      601 attcacccgc gcggcgctcg gccactgggc cggtttctcg atcgggtggc tctactggta
      661 cttctgggtg atcgtcatcc cggtcgaagc catcgccgga gccggcatca tccagcgctg
      721 gctgccgctg cccgcatggc agatcggtgc ggtgctgatg gcactgatga cggccgtcaa
      781 cctcatgtcc gcccgcgcct acggggaatt cgaattctgg ttctcatcga tcaaggtagc
      841 ggcgatcatc actttcatca tcgccgtcgg cgcttatgcg tgcggctatc actcgcccac
      901 cgggccgacc ttctcgaacc tcaccgccta cggcggcttt gcgccgcggg gggcgttagc
      961 ggtgctggca tccgtgacga ccgtgatctg gtcgatgatg ggtgccgagg tggtgacgat
     1021 tgccgccgcc gagtccccgg aacccgcgcg ggccgtggcc agaatgacct ccacgatcat
     1081 cagccgcatc gtgctctttt atgtgggctc gatcttcgtc attacctgcg tggtgccgtg
     1141 gaccaaggtc gtgccggggc agtcgccgtt cacgctggca ctcgaccaga tccattttcc
     1201 gtatgccagc acgatcatgg cggcagtgat cctcaccgcc gtgctgtcgt gcctgaattc
     1261 agcgttctat gttgcctcgc gcgtactgtt cgtgctcgcg agtcgtggcg acgcaccgca
     1321 ctggctggtg cgcaccaaca ggcgccatgt gccggctcga gccgtgctgc tcgcgagtgt
     1381 gctgggcttc gccggggtga tcgccgcgat cgtctcgccg agcggagtgt tccgcttcct
     1441 ggtcaactca tcaggtgcca tcatcgccgt catctacctc gccatcgccg tgtcacaggt
     1501 gcgcacccga agtgcacgcg aagcggcggg tgcgccaccg ccggtgctgc cgatgtggct
     1561 ctttccctgg ctcagttacc ttgccattgc gggagtggtc gccgtgctca tcgccatggc
     1621 cgtgacgcct tctcaccggg cggagttctg gaccagcgtc atctcaatgg tggtggcgct
     1681 actcgcgttc gtcgtgtttc gcctgacggc cgtcagacca tggcgaggtg cgccgcagcg
     1741 gcagccggcc acggattgag ccgcgactca gccatgatcc tgagtcgggc cgcgctgcaa
     1801 tcacgggcgc aagcctcgcg tttatcgaca ggccgcagct gaggcggcgc gcaaatgcgt
     1861 gcggctgcgg ctacgtgcag agctggggcg ggccggcgcg agcgctgaat gatctgcgtg
     1921 tgggcaactc ggtattcggt gaatgactct cattgagcgg aggtaactcg catgcgcgcc
     1981 accctcatgt accgtgctgg cgacgttcgc atcgagaacg ttcccgatcc agttctggtg
     2041 gagccaacgg acgcactcat caccatccac cgggcgtgta tttgtggaag tgacctgtgg
     2101 ccctacaagg agctccagcc cgtggagcgc ggccgccgca tgggacacga ggcggtcggg
     2161 gtggtcgagg caatcggtac ggaggtacgc cgggtcaagc gcggggacgt cgtggtgatg
     2221 ccctttgcct actccgatgg cacctgcacc ttctgtgagg cgggtctcca aacctcctgc
     2281 ctgcgcggcg gtttcttcgg agtcggcgct gaggccgctg gggcgcaagc cgaggcgctg
     2341 cgcgttccac aggcggacgg gacgctgttc gcactagccg tcggacgaga tgagcgcttg
     2401 atgccctcgt tgctcagtct ctcggacgtg atgggcaccg ggcatcatgc ggcggtgagt
     2461 gccaaagtgc gccgcggcgc aacggttgcc gtcattggcg atggcgcagt cggcttatgc
     2521 gcggtcatcg ccgcgaaacg tctgggcgca gagcaaatca tactcatggg ccgtcatccc
     2581 cagcgcatcg cactggcgcg agaattcggg gcgacggaca tcgcgcgcga gcgcggcagc
     2641 gaggtgctcg aacgagtccg gcagctgacg cagggattag gtgcgcactc ggtgctcgaa
     2701 tgtgtcggct ccgcgcaggc catggagacg gcgctggcaa tcgttcgtcc aggcggcgcc
     2761 atcggccgag tcggcgtgcc ccattattcg agcatcacgg ccggtggacc gatgttttac
     2821 aacaacgtca gcgtgagcgg cggccccgca ccggtccgcg cctacatcga ggagttgctg
     2881 ccggacatcc tcgagggccg catcgagccc ggccgcgtct tcgatcgcgt catcgggctg
     2941 gacgaggtgc cggcaggcta ccgagccatg gatgagcgtc aagcgctcaa gatcatgatc
     3001 aacccctgag tgcggcgcgg ccaacgagag gcatcatgtc gaggatactc atcaccggcg
     3061 cgaaccgcgg cctgggattg gaattcgcgc agcagtatct ggctgatggc tgggaagtca
     3121 tcgccgcgtg ccgcaatccc ggcgcggcac aagcgctgcg acggctcgcg gacagcgcca
     3181 cgggctcgct caccgtgata gcgatggacg tcgcggaccg cggcagcgtg cagcgcgccg
     3241 cgacgagccg gcaggtcgcg gcgatcgact tcgtgatcaa cagcgccggc gtcttcggcg
     3301 gcaacggaca gaggctcggt tcgttggatt accgcgactg gatgcaggtt ctcgaggcga
     3361 acctgctggg gcccgcgcgc gtgtgcgagg cttttctcga gcgcatcggc cgcagcgagc
     3421 gccggctcat cgtaaccatc accagcggca tgggctcgtt gtccgacaac acctcaggcg
     3481 gctccgtgct ctaccggacc tcgaaggcgg cggtcaacat gctgatgcgc actgccgcga
     3541 tcgatctgca gccgcagggg atcagctgcg tcgtgctcaa cccgggctgg gtgaagaccg
     3601 acatgggcgg tccgaatgcg aagatatcgg ctgcggagag tgtcggcgcc atgcgccgcg
     3661 tgatctcgag actgggccgc aaggattccg gccgctttta caactacgac ggccgcgaat
     3721 atccctggtg acgcgcgggc gcgcgctcag cgacgcctat ggagtggact tgaggtaact
     3781 gttgaaccaa ccgacgatcc gttccatcag gtcgatttgg tcgctgcgct tgaagaagtg
     3841 atgcccctca tcgggataaa cgacgaattc ggtcttgacc ccgaggcgac gcaacgcatt
     3901 ccaatattcg taggactgcg tgatgggtac ctcggcgtcc cggtcgccgc ccaccagtaa
     3961 ggtgggtgtg cggacgttct tgatgaaggt gagcggcgaa ctgcgctcgt acaccttggg
     4021 atcgtcgtac accgaagcac cgaagtacgg aatcatccag gtgtcgatgt tgttggtgcc
     4081 gtagtaactc agccagttgg aaattccggc gccggagacc gctgccttga agcgatcggt
     4141 ctgggtgagc gcccacatgg tcatgtaacc gccatagctc cagccgcaca gcccgacgcg
     4201 tgccgcatcg atcggcgcgg cgcgctcaac cgcatccaga ccgctcaata tgtcgcgcag
     4261 gtcgccgtag ccgaagtcct tgacgttc
//
LOCUS       DATDMP010000409         4625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_954856, whole genome shotgun sequence.
ACCESSION   DATDMP010000409 DATDMP010000000
VERSION     DATDMP010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954856"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VI195_07130"
     CDS             complement(<1..597)
                     /locus_tag="VI195_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6824197.1"
                     /translation="MSDAIDINKSGGVPAARRALLRQLMAVRPIDAVPEGKSRLKQVL
                     SARALIAIGLGATIGSGIFVLTGTVAANHSGPAITLSLLIAAFGGGLAALCYSEFAAF
                     LPVPGSAYSYTYATLGEGLAWFIGWNLLLEYAISASAVAVSWSAYVVSLAADAHLHLP
                     SALVNAPLGVDAHDHLIVTGAIINVPAVLIIAAMTALLY"
     gene            759..2066
                     /gene="icd"
                     /locus_tag="VI195_07135"
     CDS             759..2066
                     /gene="icd"
                     /locus_tag="VI195_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY6824198.1"
                     /translation="MSTVANLKITPPPQGAKITIQDGKLRVPENPILPFIEGDGTGPD
                     IWRASVRVMDAAVAKAYGGRRRIHWLEVFAGEKSNKLFNTWLPEETVAACREYLVSIK
                     GPLTTPIGGGIRSLNVALRQMLDLYVCLRPLRWFKGVPSPVRHPEKVDMVIFRENTED
                     IYAGIEFEYGTPENQKFLELFRQAFPKAYARIRFPETTAIGIKPVSKEGSERLIRAAL
                     AYAISHQRRSVTLVHKGNIQKFTEGAFRNWGYQLAEREFAAGTYTWEQWERTKAARGE
                     QQANAEMDAARKAGRVIVKDAIADITLQQVLTRPEEFDVIATTNLNGDYLSDALAAQV
                     GGIGIAPGGNINYLTGHAVFEATHGTAPKYANLDKVNPGSVILSGEMMLRYLGWVEAA
                     DAIIAAMDRTIGQKIVTYDFARLMEGAQEVSTSAFGDALIRNL"
     gene            2101..2730
                     /locus_tag="VI195_07140"
     CDS             2101..2730
                     /locus_tag="VI195_07140"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6824199.1"
                     /translation="MSSPPPRDLLEWARQVQAIAQNGLAFARDPFDRERYTQLAELVA
                     LLLARELQMPIDAARALWADEQGYATPKVDVRGGVFRDARVLLVRERSDGRWTLPGGW
                     VDVNDAPSEAVMREIFEESGYRARPVKLAALLDKNRHPHPAGVYHIYKLFFVCELTGG
                     AAAASAETDAVEFFPVQALPPLSTGRVLAAQIERLYQHHLDRSLPTDFD"
     gene            2755..4482
                     /gene="aceK"
                     /locus_tag="VI195_07145"
     CDS             2755..4482
                     /gene="aceK"
                     /locus_tag="VI195_07145"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEY6824200.1"
                     /translation="MSALPAPRECARHIVEAFARYNAEFRAITRRAPQRFDARDLKGS
                     QRDAVERIELYDHSVNQTIAALRLMVGAQAADRHLWRQIRAEFAAQISALPDTEFTKT
                     FFSSISRRLFGTVGVSPDIEFVATDLDPLADIHSAIGTNSYLNHGSLALLIEDLLGDV
                     RFRSPWRDLDASIAHVASEVRGYLHSRGEQRAVERVEVIRAVFYQMSRAYIVGRIQGH
                     GFLVPLVIALKNTDSGVLVDAVMLAEADVSIVFSFTRSYFHADLERVAEAVVFLRSLM
                     PRKPVSELFTVLGRARQGKTERYRELMRNLEQTQDLFEHAAGERGLVMVCFTLPSFDV
                     VFKVIRDRFPYPKTVLREEVMEKYRLVFIHDRAGRLVDAQEFRRLRFPRARFAPALLA
                     ELRRETAGTVHEDGSDLVFDHLYIERRMTPLNLYVRSAAAADAERVVLDYGQCIRDLA
                     YTNIFPGDLLLKNFGVTRHGRVIFYDYDELCSVTDCNFRDVPPATCEEDEMRGEAWFY
                     VADNDVFPETFLNFLGFNDEQRTALLRVHGEILTAGFWRGVQQRLRDGEVMEVLPYHP
                     HRVRVAGSL"
BASE COUNT          762 a         1563 c         1529 g          771 t
ORIGIN      
        1 gtacagcagc gccgtcatgg cggcgatgat cagcaccgcg ggcacgttga tgatggctcc
       61 ggtgacgatg aggtgatcgt gcgcatccac tccgagcggc gcgttcacca gagcgctcgg
      121 caggtgtagg tgcgcatccg ccgccaggct caccacgtac gccgaccagc tcaccgccac
      181 cgccgatgcc gagatcgcgt attcgagcag cagattccag ccgatgaacc aggcgagccc
      241 ctccccgaga gtcgcgtagg tgtagctgta ggcactgccg ggcacgggca ggaaggccgc
      301 gaactccgag taacacagcg ccgcgagccc gccgccgaac gcggcgatca gcagcgacag
      361 ggtgatcgca ggtcccgagt ggttggcagc caccgtgccg gtgagcacga agatgcccga
      421 gccgatggtg gcgccgagcc cgatggcgat gagcgcgcgt gcgctcagta cctgcttgag
      481 gcgcgacttg ccttccggga cggcatcgat cggccgcact gccatgagct gacgcagcag
      541 cgcacgccgc gcagcgggca cacctccgct cttattgatg tcgatggcgt cggacatgca
      601 ctgctcgttt catgctcgcc cccggggtgt cgagcgcgtg ccagtctctc atacgtggct
      661 gcactccggt acggcccggc cgcgccgcca tgccctgtgc tagcattgcg cgcccggcgc
      721 gccctgcgcc tggatttccc ctcaggagtg catccttgat gtccacggtc gccaacttga
      781 agatcacgcc gccgccgcag ggcgcgaaga tcaccatcca ggacggcaag ctgcgcgttc
      841 cggaaaatcc gatcctcccc ttcatcgagg gcgatggcac cggtccggac atctggcgtg
      901 cgagcgtgcg tgtcatggac gcggcggtcg ccaaggccta cggcggcagg cgccgcatcc
      961 actggctgga ggtgttcgca ggcgagaaat ccaacaagct gttcaacacc tggctgcccg
     1021 aagagacggt cgcggcgtgc cgcgagtacc tggtgtcgat caaggggccg ctcaccaccc
     1081 cgatcggcgg cggcatccgc tcgctcaatg tggcgctgcg gcagatgctc gatctgtacg
     1141 tgtgcctgcg accgctgcgc tggttcaaag gcgtgccctc acccgtacgg catccggaaa
     1201 aggtcgacat ggtgatcttc cgcgagaaca ccgaggacat ctacgctggc atcgaattcg
     1261 agtacggcac tcccgagaac cagaaatttc tcgagttgtt ccggcaggcc ttccccaagg
     1321 cctatgccag gatccgcttt cccgagacca ccgcaatcgg catcaagccg gtgtcgaagg
     1381 agggctcgga gcggctgatc cgcgcggcgc tcgcctacgc gatcagccac cagcgcagga
     1441 gcgtgacgct cgttcacaag ggcaacatcc agaagttcac cgagggagcc tttcgcaact
     1501 ggggctacca gctcgccgag cgcgagttcg ccgctggtac ctatacctgg gagcagtggg
     1561 agcgcaccaa ggccgcgcgc ggcgagcagc aggcgaatgc cgagatggac gcggcgcgca
     1621 aggccggccg cgtcatcgtc aaggatgcga tcgcggatat cacactgcag caggtgctca
     1681 cccgtcccga ggagttcgat gtcatcgcga caaccaacct gaacggcgac tatctctccg
     1741 atgcgctggc cgcgcaggtc gggggcatcg gtatagctcc gggcggcaac atcaactacc
     1801 tcaccggaca tgcggtgttc gaggcaaccc acggcaccgc accgaagtac gccaacctcg
     1861 acaaggtcaa tcccggctcg gtgatcctct cgggcgagat gatgttgcgc tacctcggct
     1921 gggtggaggc cgccgatgcc atcatcgccg ccatggaccg caccatcgga cagaagatcg
     1981 tcacctacga ttttgcccgt ctcatggaag gtgcacagga agtgtcgacc agcgccttcg
     2041 gcgatgcgct gatccgcaac ctgtagcgcg cggcgcggct gcggcttggg ccggcacccg
     2101 gtgagctcgc cccctccccg cgacctgctc gaatgggcgc gccaggtgca ggcgatcgcg
     2161 cagaacgggc tggctttcgc gcgcgatccg ttcgaccgcg aacgctacac gcagctcgca
     2221 gaactggtgg cgctgctgct ggcgcgtgag ctgcagatgc ccatcgatgc cgcgcgcgcc
     2281 ctgtgggcgg atgagcaggg ttatgccacc ccgaaggtag acgtgcgcgg cggggtgttc
     2341 cgcgatgcgc gcgtgctgct ggtgcgcgag cgctccgatg gccgctggac actgccgggc
     2401 ggctgggtgg acgtgaacga tgcgccctcg gaggcggtga tgcgcgagat attcgaggag
     2461 tccggctacc gggcgcggcc ggtgaagctc gcggcgctgc tcgacaagaa ccgccacccg
     2521 catcccgcgg gcgtgtatca catctacaag ctgtttttcg tgtgcgagct caccgggggt
     2581 gcggcggcgg cgagcgccga gaccgacgcg gtggaattct ttccggtgca ggcgctgccg
     2641 ccactgtcaa ccggccgggt gctggccgcg cagatcgagc ggctctacca gcatcacctc
     2701 gatcgctcgc tgccgaccga cttcgactga agcgcgcgcg cctccgccat ggacatgtca
     2761 gccctccccg ccccgcgcga gtgtgcccga cacatcgtcg aggcgtttgc gcgctacaac
     2821 gcagagttcc gggccatcac gcgccgcgca ccgcagcgct tcgacgcgcg cgacttgaag
     2881 ggcagccagc gcgatgcggt cgagcgcatc gagctgtacg accactccgt gaatcagacc
     2941 atcgccgcgc tgcgcctgat ggtcggcgcg caggccgccg accgccatct ctggcggcag
     3001 atccgcgccg agttcgccgc acagatcagc gccttgcccg atacggagtt caccaagacc
     3061 ttcttcagct ccatcagccg caggctgttc ggcaccgtgg gcgtgtcgcc ggacatcgag
     3121 ttcgtcgcga ccgatctcga tccgctcgcc gacatccact cggcgatcgg caccaacagc
     3181 tatctcaatc acggctcgct cgcgctgctc atcgaggacc tgctcggcga tgtgcgtttc
     3241 cgctcaccct ggcgcgatct cgatgccagc atcgcccacg tcgccagcga ggtgcgcggc
     3301 tacctgcact cgcgcggcga gcagcgcgcg gttgagcgcg tcgaggtgat acgcgcggtg
     3361 ttctaccaga tgtcgcgcgc ctacatcgtc gggcgcatcc aggggcacgg cttcctcgtg
     3421 ccgctggtga ttgcactcaa gaacaccgac agcggcgtgc tggtggatgc ggtcatgctg
     3481 gccgaggcgg acgtcagcat cgtgttcagc ttcacgcgct cgtactttca cgccgatctc
     3541 gagcgggtgg ccgaggcggt ggtgttcctg cgttcgctca tgccgcgcaa gccggtgagc
     3601 gagctgttca ccgtgctcgg gcgcgcccgt cagggcaaga ccgagcgcta ccgcgagctg
     3661 atgcgcaacc tcgagcagac gcaggacctg ttcgagcatg ccgccggcga gcgcgggctg
     3721 gtgatggtgt gcttcacgct gccctccttt gatgtggtct tcaaggtgat ccgcgatcgc
     3781 tttccctatc ccaaaaccgt gctgcgcgag gaggtcatgg agaagtaccg tctggtgttc
     3841 atccatgatc gcgccgggcg gctggtggac gcgcaggagt tccgccgtct gcgcttcccg
     3901 cgggcgcgct ttgcgccggc gctgctggcg gagttgcggc gcgagacggc cggtaccgtg
     3961 cacgaggacg gtagcgacct ggtgttcgat catctgtaca tcgagcggcg catgaccccg
     4021 ctcaatctgt acgtgcgcag cgctgccgcc gcggacgccg agcgcgtggt gctcgattac
     4081 ggccagtgca tccgcgatct cgcctacacc aacattttcc ccggtgacct gctgctgaag
     4141 aatttcgggg tcacgcgtca cgggcgcgtg atcttctacg actacgacga gctgtgcagc
     4201 gtcactgact gcaacttccg tgacgtgccg cccgcaacct gtgaggagga tgagatgcgc
     4261 ggcgaagcgt ggttctacgt cgccgacaac gatgtgtttc ccgagacctt cctcaacttc
     4321 ctcggtttca acgacgagca gcgcacggcg ctgctacgcg tacacggaga gatcctgacc
     4381 gcgggcttct ggcgcggcgt gcagcagcgg ctcagggacg gcgaagtgat ggaagtgctg
     4441 ccctaccacc cgcaccgcgt gcgtgtcgcc ggcagcctgt agggcgcggt agccgctgcg
     4501 ctcgagcgct cagggcctcc cggcaccacc cacccgcgcc attggctcga cggccacacg
     4561 gctgcgcgcc gccacgcgcg gctcgctgtc cgcgtcgagc tcggccaggg tgcgactcgc
     4621 ggtgt
//
LOCUS       DATDMP010000410         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_622081, whole genome shotgun sequence.
ACCESSION   DATDMP010000410 DATDMP010000000
VERSION     DATDMP010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622081"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..>2488
                     /gene="aceE"
                     /locus_tag="VI195_07150"
     CDS             177..>2488
                     /gene="aceE"
                     /locus_tag="VI195_07150"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY6824201.1"
                     /translation="MTEPPKLEDLDPVETREWLESIDSVLKIHGPERAHYLLERLIDY
                     TRRHGAYLPFKPNTAYVNSIPVGREPEYPGNRALERRIEAYIRWNALMMVLLANKKST
                     ELGGHLSSYASSATLYEVGFNHIWRAPSERHPGDMLFIQGHSAPGIYGRAFLEGRLTQ
                     EQLRHFRQETGGPGVGLSSYPHPWLMPEFWQFPTVSMGLGPMMAIFQARFIRYLEHRG
                     LVKPSDRKVWAFLGDGEMDEPESMGALTMPVREGLDNLIFVINCNLQRLDGPVRGNGK
                     IIQELEAAFLGAGWNVIKVLWGSRWDPLLARDHLGLLRKAMEETVDGEYQNFKAKGGA
                     YTREHFFGKYPELKAMVANMSDDEIWRLNRGGHDAQKVYAAYAAAMHHKGQPTVILAK
                     TVKGYGLGKGGEGQMVAHQQKKLSEEDLRAFRDRFNIPVSDTELASLSFRTFGQDSEE
                     ARYLSSKRAELGGSMPMRRHSAPALPVPPLQAFAALLEGTGEREISTTMAFVRILTVL
                     LKDKNIGRNVVPIVPDEARTFGMEGLFRQIGIYSSVGQLYTPQDAETLMSYREDKKGQ
                     MLEEGINEAGSLCSWMAAGTAYSNHGINMVPFFIFYSMFGFQRVGDFMWAAGDMQARG
                     FLLGATSGRTTLAGEGLQHQDGHSQLLATTVPNCVAYDPTFAYELAVIIQDGLRRMYA
                     EQQNIFYYITVMNENYAQPPLPSGAEAGILKGGYLLHTGGRGKVRATLLGSGTILREC
                     LAAAKILENDYGVPADVLSITSFSELRREAL"
BASE COUNT          443 a          835 c          794 g          416 t
ORIGIN      
        1 cgctcgtgag cgcgaccgcc ggcgcgcacg cggacgcttg ccgacctcac ttgctttacc
       61 tgtcgccgct cgccatcatt ggtcattcta acgcgacagg gcacatcacg cgcgcgaccg
      121 cggtcgcgac cgtgccggca ggccgcccgg atcgcgccgc tcgcgagcac tccgtaatga
      181 ccgaaccgcc gaagcttgag gatctcgatc ccgtcgaaac gcgggagtgg ctggagtcga
      241 tcgactcggt gctgaagatc cacggcccgg aacgcgcgca ctacctgctc gagcggctca
      301 tcgactacac gcggcgccat ggcgcttacc tgccgttcaa gcccaacacc gcctacgtca
      361 acagcattcc cgtcgggcgc gagccggagt acccgggcaa ccgtgcgctc gagcggcgca
      421 tcgaggcgta catccgctgg aacgcgctga tgatggtgct gctcgccaac aagaaatcca
      481 ctgagttggg cggtcacctc tcgagctacg cctcctcggc gacgctctac gaggtgggct
      541 tcaatcacat ctggcgtgcg ccctccgagc gccatccggg cgacatgctg ttcatccagg
      601 gacactccgc accgggcatc tacgggcgtg ccttcctcga gggacgcctg acgcaggagc
      661 agctgcggca cttccgccag gagaccggcg gcccgggtgt tggcctgtca tcgtatccgc
      721 acccctggct gatgccggaa ttctggcagt ttcccacggt gtcgatgggg ctgggtccga
      781 tgatggcgat cttccaggct cgcttcatcc gctatctcga gcaccgcgga ctcgtgaagc
      841 cctccgaccg caaggtatgg gcgttcctcg gcgatggtga gatggacgag cccgagtcga
      901 tgggcgccct caccatgccg gtgcgcgagg gactcgacaa cctgatcttc gtcatcaact
      961 gcaacctgca gcgtctcgat ggcccggtgc gcggcaacgg caagatcatc caggagctgg
     1021 aagccgcgtt tctcggcgcc ggctggaatg tcatcaaggt gctgtggggc tcgcgttggg
     1081 atccgctgct ggcacgtgac catctcggcc tgctgcgcaa ggcgatggag gagaccgtcg
     1141 acggcgagta ccagaacttc aaggcaaaag gtggcgccta cacccgcgag catttcttcg
     1201 gcaagtaccc cgagctcaaa gcgatggtcg ccaacatgtc ggacgatgag atctggcggc
     1261 tcaaccgcgg cgggcacgat gcgcagaaag tgtatgccgc ctatgcagcc gccatgcatc
     1321 acaaggggca gccgaccgtc atcctcgcca agaccgtcaa aggctatggt cttggcaagg
     1381 gcggcgaagg ccagatggtg gcgcaccagc agaagaagct ctcggaagag gacctgcgcg
     1441 ccttccgcga ccgcttcaac atcccggtga gcgacaccga gctcgcgagc ctttccttcc
     1501 gcacgttcgg gcaagactcc gaggaggcgc gttacctctc cagcaagcgc gccgagctcg
     1561 gcggctcgat gccgatgcgg cggcacagcg cgccggcact tccggtgccg ccgctgcagg
     1621 cgttcgcagc gctgctcgag ggcaccggtg agcgcgagat ctccaccacc atggcgttcg
     1681 tgcgcatcct caccgttctc ctcaaggaca agaacatcgg ccgcaacgtc gtgccgatcg
     1741 tgccggacga ggcgcgcacc ttcggcatgg agggcctgtt ccgccagatc ggcatctact
     1801 catcggtggg gcagctctac accccgcagg acgccgagac cctcatgtcc taccgcgagg
     1861 acaagaaggg gcagatgctg gaggagggca tcaacgaagc ggggtcgctg tgctcatgga
     1921 tggcagcggg caccgcttac agcaatcacg gcatcaacat ggtgccgttc ttcattttct
     1981 actcgatgtt cggcttccag cgcgtcggcg atttcatgtg ggcggccggc gacatgcagg
     2041 cgcgcggctt cctgctcggc gccacctccg gccgcaccac gcttgccggc gaggggctgc
     2101 agcaccagga cggccacagt caactgctcg cgaccacggt gcccaactgc gtcgcctacg
     2161 atccgacctt cgcttacgag ctcgcggtga tcatccagga tggcctgcgt cgcatgtatg
     2221 ccgagcagca gaacatcttc tactacatca ccgtgatgaa cgagaactac gcgcagccgc
     2281 cgctgccgag cggcgccgag gcgggaattc tcaagggcgg ctatctgctg cacacgggcg
     2341 ggcgcggcaa ggtgcgcgcg acgctgctcg gctcgggcac catcctgcgc gagtgtctcg
     2401 ccgcggcgaa gattctcgag aacgactacg gcgtgccggc ggatgtgctc tccatcacga
     2461 gcttcagcga gctgcgccgt gaggcgct
//
LOCUS       DATDMP010000411         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_733800, whole genome shotgun sequence.
ACCESSION   DATDMP010000411 DATDMP010000000
VERSION     DATDMP010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733800"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..559)
                     /locus_tag="VI195_07155"
     CDS             complement(269..559)
                     /locus_tag="VI195_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824202.1"
                     /translation="MDTESPPLDVVAKVLSRPLLCHTHFLLDLFQRAEQQHPRCGARG
                     GLDAWLCPVQVITVALDAFYRARRHADGEQLELVAGLREVLRKPVIEFRVSL"
     gene            complement(818..1579)
                     /gene="istB"
                     /locus_tag="VI195_07160"
     CDS             complement(818..1579)
                     /gene="istB"
                     /locus_tag="VI195_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY6824203.1"
                     /translation="MVQARVLENLARLRLRHLVDRLDALLAEAARGEPTYLDFLDSIL
                     RGEVDSKQRKRVAMGLTIAHFPSVKTLDDFDFKSQPSIDQKLVRELAAGRFISQAENV
                     LLFGPPGVGKTHLAIALGRAVVEAGHSVLFTSATALLGALSRAEAEGQLADKLAFYAK
                     PKLLIIDELGYLPFEKRSAHLFFQLVARRYEKASLLITTNQLVGQWGQVFGDDVLAAA
                     ILDRLLHHSHALVIQGESYRLRMKRKAGILGGERR"
     gene            complement(1585..2817)
                     /gene="istA"
                     /locus_tag="VI195_07165"
     CDS             complement(1585..2817)
                     /gene="istA"
                     /locus_tag="VI195_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY6824204.1"
                     /translation="MVAAEQVKQMGQLAELGWGAKRIARELGLARNTVRRYLRGARAE
                     VQEHLAARRLDEAARAEALRLFDGEAEGNAVVVARLLQDRGIVASVRTVQRAVEDRRR
                     SRRASQVATVRFETAPGEQMQVDVGQRLVRIAGALVRVFFVCAVLGFSRRLSVRAFLS
                     ARQDDWREGIAGAFQRFGGVPRTLLIDNDGALVIGRDEATQTARLHPAFAAFCRDFGV
                     EARVCRPYRARTKGKTESGVKYVKRNAIAGRDFGSFAEFEGHLDGWMREADERVHGTT
                     HERPIERFLRAEAAALRPLPANPLPLRERRLRRRVANDALVDVDTVRYSVPHRLVRTD
                     VEVLVAESEVRIYSGAELVATHVRTREPHTSVVDFAHYRGLWRAPPQLPPAEPPRALE
                     ALGRSLAEYEAALLGGSR"
BASE COUNT          551 a         1038 c         1030 g          512 t
ORIGIN      
        1 atgcgtcgcg tgtcctttag aaacgccagg aagcgcttga tggcctcaat ttcgtagtca
       61 gccttcatca gaagtcatcg tacttgcgcg gacgtggaag cgcactccgt ttcctgccac
      121 gcccagacga agctgttttc gccctcgacc gagtccgaac atgaatggca tcaagtcagc
      181 atcttgacga cttgaacgat gtagttggtt acgagcagct tactgagcaa gactctggtg
      241 tcggagcatc gcccgtacga atcgagcctc aaagcgagac tctaaactcg atcaccggct
      301 tgcgaagtac ctcccgaaga ccagcgacga gttccagctg ttcaccgtcc gcgtgccggc
      361 gggcacggta gaacgcatcc agcgctaccg tgatcacctg aaccggacac agccaggcat
      421 ccaggccacc gcgagcaccg cagcgcggat gctgctgttc cgcgcgctgg aacaggtcga
      481 ggaggaaatg ggtgtggcaa agaagcggtc gagagagcac cttcgcgaca acgtcaagcg
      541 gaggtgactc cgtatccacg agttcatcaa tccgagagtc tcacgtggat gaactcgtta
      601 ctgccctttg gcatgtagta gacgccatcg gcactctctt cgtagaagag atcgatagca
      661 tcccgctgag tcactagcac ctcaggctcc tgcggctcgc actcgaagta ccccttcccg
      721 catgcagtgc gatagcgacc tggcggcact tgtccagcga cattgctttc tgacccccaa
      781 acgacagaac ttctgacccc ctgtttgagt cggactgcta ccgcctctcg cctccgagaa
      841 tgccggcctt gcgcttcatc ctgagccggt aactctcgcc ctggatgacg agggcgtggc
      901 tgtggtggag gagccggtcg aggatggcgg cagcgagcac atcgtcaccg aagacctggc
      961 cccattgccc aacgagctgg ttggtggtga tgagaagact ggccttctcg tagcggcgag
     1021 cgacgagctg aaagaagaga tgtgcggacc gcttctcgaa gggcagatac ccgagctcgt
     1081 cgatgatgag cagcttgggc ttggcgtaga aagcgagctt gtcggcgagc tggccttcag
     1141 cttctgcgcg ggagagcgca ccgagcagcg cggtggcgct ggtgaagagc accgagtggc
     1201 ccgcctcgac gacagcgcgc ccgagcgcaa ttgcgaggtg cgttttgccg acgcctgggg
     1261 gcccgaagag cagcacattt tccgcctgcg agatgaaacg cccggcggcg agctcccgca
     1321 cgagcttctg gtcgatggag ggctgcgact tgaagtcgaa gtcatcgagc gtcttcaccg
     1381 agggaaagtg agcgatggtc aggcccatcg cgacccgctt gcgctgcttg gagtcgacct
     1441 cgccgcgcag gatggagtcg agaaaatcga ggtaggtcgg ctcgccgcgg gcggcctccg
     1501 cgagcagcgc atcgagccga tcgacgagat ggcggaggcg caggcgcgcg agattctcca
     1561 acacgcgcgc ctggacgatg gcgctcatcg cgagcctccg aggagcgcag cctcgtactc
     1621 ggcaaggctg cggccgagcg cctcaagggc gcggggtggc tctgccggcg ggagttgcgg
     1681 cggcgcgcgc cagagtccgc ggtagtgggc aaagtcgacg acgctggtgt gaggctcgcg
     1741 tgtccgtacg tgcgtcgcga ccagctccgc accgctgtag atgcggacct cagactcggc
     1801 gacgagcacc tcgacgtcgg tgcgcaccag tcggtgcggg acgctgtagc ggaccgtatc
     1861 gacatcgacg agcgcgtcgt ttgcgaccct gcggcgcagg cgccgctcgc gaagcggcag
     1921 cgggtttgcc ggcagcggcc gcagggcagc ggcttcggca cgcaggaaac gctcgatggg
     1981 ccgctcgtgc gtggtgccgt ggacgcgctc gtccgcctca cgcatccaac cgtcgaggtg
     2041 gccctcgaac tcggcgaagg agccgaagtc gcggccagcg atggcgtttc gtttgacgta
     2101 cttcacgccc gactcggtct tgcctttggt gcgtgcgcgg tacggccggc agacgcgcgc
     2161 ctcgacgccg aagtcgcggc agaaggcggc gaaggcggga tgcagccggg ccgtctgcgt
     2221 cgcctcatcg cggccgatca ccagcgcgcc gtcattgtcg atgagcagcg tccgcggaac
     2281 gccgccgaag cgctggaagg cgccggcgat gccctcgcgc cagtcgtcct gcctggcgct
     2341 caggaacgcg cgcaccgaca gccgccgcga aaacccgagc acggcgcaga cgaagaacac
     2401 gcgcaccagc gcgcctgcga tgcgaaccag gcgctgcccg acgtccacct gcatctgctc
     2461 gcccggggcg gtctcgaacc gcaccgtcgc gacctgggaa gcgcgccggc tccggcgccg
     2521 gtcctcgacc gcccgctgca ccgtgcgcac gctggcgaca atgccgcggt cctggagcag
     2581 ccgcgccacc accaccgcat tgccctcggc ctcgccgtcg aagagccgca gcgcctctgc
     2641 tcgagcagcc tcgtccagcc gtcgcgccgc caggtgctcc tgcacctcgg cccgcgcccc
     2701 gcgaaggtag cgtctcaccg tgttgcgcgc gaggcccagc tcccgagcga tccgcttggc
     2761 accccagccc agctccgcca gctgtcccat ctgcttgacc tgctccgccg cgaccatcgg
     2821 cacctccacc ggcagaggtg caccgctcct gacctgtcga tccatttacc gccctccgtt
     2881 tggagggggt cagaagtact gtcgcgaggg ggtcaatagc tgtgtcgccc tacagcatcg
     2941 ccacgcactt ttcgtacttc gcgcgcacgg tctgcttctt ggcccgcagc catagcgatg
     3001 ggttgaggcg cgcccgcccg ctgcgccgga gggtgcgcag cgtcggtctg gagcagcgga
     3061 ttcgccggct gcgggcggcg gggaatgagc ggatgagcca tacagacagt ccaccgatgt
     3121 gcgctcaatc a
//
LOCUS       DATDMP010000412         3824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_289495, whole genome shotgun sequence.
ACCESSION   DATDMP010000412 DATDMP010000000
VERSION     DATDMP010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289495"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VI195_07170"
     CDS             <1..242
                     /locus_tag="VI195_07170"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6824205.1"
                     /translation="VMQSGAQPVTIDYDMEKRPGGWKVWDVRVAGVSLVANYRTEFDT
                     QVRDNGIDGLIRTLQAKNRSLEQPAATASSGGVKK"
     gene            239..535
                     /locus_tag="VI195_07175"
     CDS             239..535
                     /locus_tag="VI195_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6824206.1"
                     /translation="MIRREGDVMRVSGALTLASVTADFAQGKSAIAEGARSADLAGVG
                     ELDSSALALLLAWLREAKRLGFELTFANLPQGLTTIARLYGVAELLPTAAASHH"
     gene            601..1539
                     /locus_tag="VI195_07180"
     CDS             601..1539
                     /locus_tag="VI195_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6824207.1"
                     /translation="MTIAIEALDVEKRYGALHALAGVSLAVAEGEFFGLLGPNGAGKT
                     TLINVIAGLARASAGTVRVMGADVIADYRRARRMLGVVPQELVFDPFFTVRETLRLQS
                     GYYGLRQNDAWIDEVMHHLDLTAKADANMRALSGGMKRRVLVAQALVHRPPVIVLDEP
                     TAGVDVGLRQGLWQFIRRLNRDGHTIVLTTHYLEEAEVLCDRIAMLKAGRIVALDSTR
                     NLLENFSGLLLRLRLDCDALPAGIDGELVASGEGRFALRLRDYTALEQVLHRVREAGA
                     TIRELELEPPDLEEVFLRVMGTGETSASPAPPSEKP"
     gene            1539..2294
                     /locus_tag="VI195_07185"
     CDS             1539..2294
                     /locus_tag="VI195_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824208.1"
                     /translation="MGGFPTLLYKELLRFWKVSFQTVAAPVLTALLYLVVFAYALGDR
                     VRVFEHTSYAQFLVPGLVMMSVLQNAFANSSSSLIQSKISGNLVFMMLPPISYLEFFG
                     AYVLASMVRGLVVGGGVLAVTVWFVDLHVASPAWVLAFALLGAAVLGALGLIAGIWAD
                     KFDQIAAFQNFVILPFTFLSGVFYSIHSLPAAWQSASHLNPFFYMIDGFRYGFFGASD
                     FPPLASLAVVAVSFALLGGAALALLRSGYKLRQ"
     gene            2297..2545
                     /locus_tag="VI195_07190"
     CDS             2297..2545
                     /locus_tag="VI195_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY6824209.1"
                     /translation="MVTPESVKSGIEAGLECDSVEVIGDGQHFQARIVSRAFAGKSRV
                     QRHQLVYRALGERMREEIHALSMQTLTPEEAAGEARDG"
     gene            2538..3788
                     /gene="murA"
                     /locus_tag="VI195_07195"
     CDS             2538..3788
                     /gene="murA"
                     /locus_tag="VI195_07195"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY6824210.1"
                     /translation="MDKLRITGGRPLRGAVPISGAKNAALPVMAATLLVAGVHRLRNV
                     PRLRDTRTFARVLELLGAKVAFDGNLCTIDSTGVNSVEAPYELVKTMRASIYVLAPLL
                     ARFGEARVSLPGGCAWGPRPVNLHLSGLQAMGAGLDIEHGYIVGRKVKLKGATFAFDV
                     VSVGATAQLMMAAVLADGTTVLENVAIEPDIIALGEVLVACGARIEGLGTRQLSIRGV
                     RSLQPIEATIIPDRIEAATFAAAAAMTGGQLKLEGVVPAHFDAVLLKLEEAGSTVTRA
                     ADSVTVTGPARPKAVNVVTQPFPGFPTDMQAQMIAVSAIAVGTSVIEDTVYLDRFTHV
                     PELARMGASIELKGNVAVVKGVEGLSGAPVMATDLRASASLVLAGLVAQGETVIDRIY
                     HLDRGYEALETKLAALGARIQRVE"
BASE COUNT          564 a         1297 c         1351 g          612 t
ORIGIN      
        1 gcgtgatgca gtccggcgcg cagcccgtga ccatcgacta cgacatggaa aagcggcctg
       61 gcggctggaa ggtctgggac gtgcgcgtcg ccggggtcag cctggtcgcg aactaccgca
      121 ccgagttcga cacccaggtg cgcgacaacg gcatcgacgg gttgatcagg actctgcagg
      181 cgaagaaccg ctcgctcgag cagcccgccg ccaccgccag cagcggcgga gtgaagaaat
      241 gatccggcgc gagggcgacg tgatgcgggt gagcggggcg ctgacgctcg ccagcgtcac
      301 cgccgatttt gcgcagggca agtcggccat tgccgagggc gcgcgcagcg ccgacctcgc
      361 cggcgtcggc gagctggatt cgtccgcgct cgcgctgctg ctcgcctggc tgcgcgaagc
      421 gaagcggctc ggcttcgaac tgactttcgc gaacctgccg cagggcctga cgacgatcgc
      481 gcgcctgtac ggcgtggccg agctgctgcc caccgcggcg gcgtcccacc actgagctga
      541 acgcccgcgc gaatttgctg cggcagctgg ccgcggcacg tcgtagcggg caagcccgcc
      601 atgaccattg cgattgaagc cctggatgtc gaaaagcgct acggcgcgct gcacgcgctc
      661 gccggggtca gcctcgccgt cgccgagggc gaattcttcg gcctgctcgg tcccaatggc
      721 gccggcaaga ccacgctgat caacgtgatt gcgggcctgg cgcgcgccag cgcgggcacg
      781 gtgcgcgtga tgggcgccga cgtgatcgcc gactaccggc gcgcgcggcg catgctcggc
      841 gtggtgccgc aggagctggt gttcgatccc ttcttcaccg tgcgggaaac gctgcgcctg
      901 caatccggct actacggcct gaggcagaac gacgcctgga tcgacgaggt gatgcaccac
      961 ctggatctca ccgccaaggc cgacgccaac atgcgcgccc tgtcgggtgg catgaagcgg
     1021 cgcgtgctgg tcgcgcaggc gctcgtgcac cggccgccgg tgatcgtgct cgacgagccg
     1081 accgcgggcg tggacgtcgg attgcgccag ggcctctggc agttcatccg gcgcctgaac
     1141 cgcgatggcc ataccatcgt gctgacgacg cattacctcg aggaagccga ggtgctgtgc
     1201 gaccgcatcg cgatgctcaa ggcgggccgc atcgtcgcgc tcgattcgac gcgcaacctg
     1261 ctcgagaact tttcgggcct gctgctgcgg ctgcgcctgg actgcgacgc gctgccggcc
     1321 ggcatcgacg gcgagctcgt ggcgagcggg gagggccgct tcgcgctgcg cctgcgcgac
     1381 tacaccgcgc tcgagcaggt gctgcaccgc gtgcgcgagg cgggcgccac gatccgcgag
     1441 ctggagctcg agccgcccga cctcgaagag gtctttctgc gcgtcatggg aacaggggaa
     1501 acaagtgctt cccccgcacc cccttcggag aagccctgat gggcggcttc ccgacgctgc
     1561 tctacaagga gctgctgcgc ttctggaaag tgagcttcca gaccgtggcc gcgcccgtgc
     1621 tcaccgcgct gctgtacctg gtggtgttcg cctacgcgct cggcgaccgc gtgcgcgtgt
     1681 tcgagcacac gagctacgcg caattcctgg tgccgggcct ggtgatgatg agcgtgctgc
     1741 agaacgcgtt cgccaacagc tcctcaagcc tcatccagtc gaagatttcc ggcaacctgg
     1801 tgttcatgat gctgccgccg atctcgtacc tggagttctt cggcgcctac gtgctcgcct
     1861 cgatggtgcg cggcctggtg gtgggcggcg gcgtgcttgc ggtgacggtc tggttcgtcg
     1921 acctgcacgt cgcgtcgccc gcctgggtgc tcgccttcgc gctgctcggc gcggcggtgc
     1981 tcggcgcgct cggcctgatc gccggcatct gggccgacaa gttcgaccag atcgccgcgt
     2041 tccagaactt cgtcatcctg ccgttcacct tcctgtccgg ggtgttctac tcgatccatt
     2101 cgctgccggc ggcctggcag tcggcctcgc acctgaaccc gttcttctac atgatcgacg
     2161 gcttccgcta cggcttcttc ggcgcgtccg acttcccgcc gctcgcgagc ctggcggtgg
     2221 tcgcggtaag cttcgcgctg ctcggcgggg cggcgctcgc gctgctgcgc tcgggctaca
     2281 aactcaggca ataaccatgg tgactcccga gagcgtgaaa agcggcatcg aagccggcct
     2341 cgaatgcgac agcgtcgagg tgatcggcga cggccagcac ttccaggcgc gcatcgtgtc
     2401 gcgcgccttc gcgggcaaga gccgcgtgca gcgccatcag ctggtctacc gcgcgctcgg
     2461 cgagcgcatg cgcgaagaga tccatgcgct gtcgatgcag acgttgacgc cggaagaagc
     2521 ggccggggag gcgcgggatg gataagctgc gcatcaccgg cgggcggccg ctgcgcggcg
     2581 cggtgccgat ctcgggcgcc aagaacgcgg cgctgccggt gatggccgcg acgctgctgg
     2641 tggcgggcgt ccaccgcctg cgcaacgtgc cccgcctgcg ggacacgcgc accttcgcgc
     2701 gggtgctgga gctgctgggc gccaaggtcg ccttcgacgg caatctctgc accatcgact
     2761 ccaccggcgt gaactcggtg gaggcgccct acgagctcgt caagacgatg cgcgcgagca
     2821 tctacgtgct ggcgccgctg ctggcgcgct tcggcgaggc gcgcgtatcg ttgcccggcg
     2881 gctgcgcctg gggaccgcgg ccggtgaacc tgcatctgtc gggactgcag gcgatgggcg
     2941 ccgggctcga catcgagcat ggctacatcg tcggacgcaa ggtgaagctg aagggcgcca
     3001 cgttcgcctt cgacgtggtt tcggtcggcg cgaccgcgca gctcatgatg gcggccgtgc
     3061 tcgcggacgg cacgacggtg ctcgagaacg tcgccatcga gcccgacatc atcgcgctgg
     3121 gcgaagtgct cgtggcctgc ggcgcgcgca tcgagggcct gggaacgcgc cagctcagca
     3181 ttcgcggcgt gcgatcgctg cagccgatcg aagcaaccat cattcccgac cgcatcgagg
     3241 cggcgacctt cgccgccgcc gccgcgatga ccggcgggca actgaagctc gagggcgtcg
     3301 tgcctgcgca tttcgatgcg gtgctgctca agctggagga ggccggcagc acggtcacgc
     3361 gcgccgcgga ttcggtgacg gtcaccggtc cggcgcggcc gaaggcggtg aacgtcgtga
     3421 cgcagccgtt tcctggcttc ccgaccgaca tgcaggcgca gatgatcgcg gtcagcgcga
     3481 tcgccgtcgg caccagcgtg atcgaggaca ccgtgtatct cgaccgcttc acgcacgtgc
     3541 ccgaactcgc gcgcatgggc gccagcatcg agctcaaggg caatgtcgcg gtggtgaagg
     3601 gcgtcgaagg gctttccggc gcgcccgtga tggcgaccga cctgcgcgcc tcagcgagcc
     3661 tcgtgctcgc gggcctggtt gcgcagggcg agaccgtgat cgaccgcatc taccacctcg
     3721 accgcggcta cgaggcgctc gagacgaagc tggccgcgct cggcgcgcgc atccagcggg
     3781 tcgaatgagc gccgcaccgg aaggtacact gcatcgcatg ggca
//
LOCUS       DATDMP010000413         4190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2066885, whole genome shotgun sequence.
ACCESSION   DATDMP010000413 DATDMP010000000
VERSION     DATDMP010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066885"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..454)
                     /locus_tag="VI195_07200"
     CDS             complement(170..454)
                     /locus_tag="VI195_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6824211.1"
                     /translation="MTRIYVGNLPFSATDAEIRELFAQHGTVESVSIITDRDTGRPRG
                     FGFVEMSRADASRAIQNLNGKELGGRPLRVNEAQERSAGGNRGGGAPKRW"
     gene            complement(620..817)
                     /locus_tag="VI195_07205"
     CDS             complement(620..817)
                     /locus_tag="VI195_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824212.1"
                     /translation="MFIFSRPFRAVAGGLSVALLTVCLDRACEAPGTGSAALPAAAQT
                     AKAPSPACAAPVACGIVRAGS"
     gene            941..1870
                     /locus_tag="VI195_07210"
     CDS             941..1870
                     /locus_tag="VI195_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6824213.1"
                     /translation="MDQLACMRSFVRVADLHSFTKAAEALGLSRAVISTHVAELESHL
                     RCQLLHRTTRRVGLTADGAEYLTRCQRILAELEAADESVRRTRLSPEGRLRVDVPVEF
                     GRALLIPALPQFTARYPDLQLEVQFNDRVVDLIREEIDLVVRVGAVREPHLIARRVVS
                     TRLLTCASPEYLRSHGAPATPEDLRRHKLVGYLPTGTRRPHRWVFERGTQRMRLGLPF
                     NIAFNSAEAEIRAAIRGAGLVQAMDLVVAEALALGRLQVVLPDWSAPGKPISVVCRAA
                     LRDSPKIRVFAEFAAELLRRYRQRVDALLDLPG"
     gene            complement(1859..>4190)
                     /locus_tag="VI195_07215"
     CDS             complement(1859..>4190)
                     /locus_tag="VI195_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEY6824214.1"
                     /translation="VIVTQTVTLAAVLLSTRRTVEARAAEELRAGGTLAEHLIGFRAG
                     QLANGVGVLAGDFGFREAVADGHTPTILDAAANQAQRIGADMVLVLDTHGRLLASTAA
                     AAEDAEGSLAGLLEDAGDLRDQPNFRVFGAHSYQFFLAPVRAPQTIAWVAMGFVADKA
                     LAARIRDLVGSEVAIVTHGAAGVTRIASTLPPAAAALYPAASELPHITRLGAADYLSF
                     ARRLDARGDSVEVILLKPLEDVLAPYRQQRDAMLLIDGVALLLAALIGAVLGRSATRP
                     IGELVRAAQRIERGQYGIAVDVAGGEEFRSLASTFNAMQSNIAAREADITYHAYHDPL
                     TALPNRAFAKRTLESLIGAGASEPGVALLLIELRNLPDINASLGLQVGDEVLREAARR
                     LQQNIAASETVARLGEMQFLVVAPGCTAERAPLYAGQLAAVIRGGFHLAGVSLDVRLA
                     CGVCLFPLHGASADELLQRVQVALEDADEARTRVALYRPGQDQEHRRRLALVTDLRRA
                     IDQDQLSLVYQPKVAMASRSVRSLEALARWTHPELGAVSPAEFVPLAESTGGSRQLTN
                     WVLGAAIRQMGEWRRQGLELDLAVNLSAPDVLDPDLSDEILRLLRRHQVDSTSLLLEI
                     TESAVMRDPQVAVRNMQWLRIAGIRFAIDDFGTGHSSLSQLSVLPVDELKIDRSFMSP
                     ADPAAVTIVTSTIELGHSMGLKVVAEGVENAEAWNLLRRLGCDFAQGYLISPPLAPAQ
                     VPGFVSQANQVLPDSDSTVRQIRALDQLAGRTHSSR"
BASE COUNT          682 a         1430 c         1438 g          640 t
ORIGIN      
        1 gggtccttgc ggcgcgcttt gccgaaggag ccgcgataga tcttgccgcg gcgtgttctc
       61 tgatcgcctt tacccatttg cagcctctcc cgatgtccca cgaaaaaggc cggcgaccgc
      121 tgcccgcggg ggcagcggca cacgccggcc tcgagcagct ctgcgaagct taccagcgct
      181 tcggtgcgcc gccgccgcgg ttgccgccgg cgctgcgctc ctgagcctcg ttgacccgca
      241 gcgggcggcc gccgagctcc ttgccattga gattctggat ggcgcgcgag gcgtcggcgc
      301 gcgacatctc gacgaagccg aagccgcgcg ggcggccggt gtcgcgatcg gtaatgatgc
      361 tcaccgactc gaccgtacca tgctgtgcga acagctcacg gatctcggca tcggtagcgg
      421 aaaaaggcaa gttgcccaca tagattctcg tcatgcaatc aaactcctga agcgaacggt
      481 atcagcagga gcgcatgccg aaaatcgcgg actcgtcggc tcacaccctg ccacctggtc
      541 gggaacgatt cggcttttca gactcggaag ccaactcgcg gtctaaagtg cgggcggcag
      601 gcggcaggaa agggaaaggt cacgaaccgg cgcggacgat accgcaagcg accggcgcgg
      661 cacaagcggg cgagggcgct tttgcggtct gcgccgcggc gggaagcgca gcgctaccgg
      721 tgccgggtgc ctcgcaggca cgatcgaggc acaccgtcag caacgccacg gaaagcccgc
      781 cggcgactgc gcggaacggc cggctgaaga tgaacatgac tcactcctca cgctgtggac
      841 tgcggcgaca gtccgtgcct gcaaacggta gccccgtgtg ttatctgcag aaactgtcca
      901 atcgcgaaat tattgttcgt tattgccata caatagggga atggatcagc tcgcctgcat
      961 gcgcagcttc gtgcgcgtgg ccgacctgca ctccttcacc aaggcggcgg aggcgctcgg
     1021 cctgtcgcgc gcggtcatca gcacacacgt cgccgagctc gagagtcacc tgcgctgtca
     1081 gctcctgcac cgcacgaccc ggcgcgtcgg actgaccgcc gacggcgccg agtacctcac
     1141 gcgctgccag cgcattctgg ccgagctcga ggcggcggat gagtcggtgc gccgcacccg
     1201 cctctcgccc gagggccgcc tgcgggtcga tgtgccggtg gagttcggcc gcgcgctgct
     1261 gatcccggca ctgccgcaat tcaccgcgcg ctacccggat ctgcagctcg aggtgcagtt
     1321 caacgatcgc gtggtcgacc tgatccgcga ggagatcgac ctggtggtgc gtgtgggggc
     1381 ggtgcgcgag ccgcatctca tcgcacgccg cgtggtgagc acgcggctcc tcacctgtgc
     1441 ctcgccggag tatctgcgca gccatggcgc ccccgcgacg cccgaggacc tgcgccgtca
     1501 caagctcgtc ggttatcttc cgaccggcac ccgccggccg caccgctggg tgttcgagcg
     1561 gggtacgcag cgcatgcggc tcggactgcc gttcaacatc gccttcaatt ccgccgaggc
     1621 ggagatccgc gccgcgatcc gtggcgccgg cctggtgcag gcgatggatc tcgtcgttgc
     1681 ggaagctctc gcactcggcc gcctgcaggt ggtgctgccg gactggtcgg cgcccggcaa
     1741 gcccatctcg gtggtgtgcc gcgcggcgct gcgcgattcg ccgaagatcc gtgtgttcgc
     1801 cgagtttgcc gcggagctgc tgcggcgtta ccggcagcgc gtcgacgcgc tgctcgactt
     1861 accgggatga gtgggtgcgc ccggcgagct gatcgagggc gcggatctga cgcacggtcg
     1921 aatcggagtc gggcaacacc tggttggcct gactgacgaa cccgggaacc tgcgccggcg
     1981 cgagcggcgg gctgatgagg tagccctgcg cgaagtcgca accgaggcgg cgcagcagat
     2041 tccaggcttc ggcgttctcc accccctccg ctaccacctt caaccccatg ctgtgaccga
     2101 gctcgatggt cgaggtgacg atggtgaccg cggcggggtc ggcgggcgac atgaaggagc
     2161 gatcgatctt cagctcatcc accggcaata ccgacagctg cgacagcgac gaatgcccgg
     2221 taccgaagtc atcgatggcg aagcgtatgc cggcgatgcg cagccactgc atgttgcgca
     2281 ccgcgacctg aggatcgcgc atcaccgcgc tctcggtgat ctcgagcagc agtgaggtgg
     2341 aatcgacctg atggcggcgc agcagccgca ggatctcatc gctcaggtcc ggatcgagta
     2401 catcgggtgc cgacaggttc accgcgagat cgagctcgag gccctgccgg cgccactcgc
     2461 ccatttggcg gatcgcggcg ccgagcaccc agttggtgag ctgacgcgag cctccggtgc
     2521 tctcggcgag cggcacgaac tccgctggtg agaccgcgcc gagctcggga tgcgtccagc
     2581 gcgccagtgc ctcgaggctg cgcaccgagc ggctcgccat ggcgaccttc ggctggtaca
     2641 cgaggctcag ctgatcctga tcgatggcgc gacgcagatc ggtaacgagt gccaggcggc
     2701 ggcgatgttc ctgatcctgg ccgggacggt acagggcaac gcgcgtgcgc gcctcatccg
     2761 catcttccaa cgcgacctgc acacgctgca gcagctcatc ggcgctcgcg ccgtgcagcg
     2821 gaaacagaca cacgccgcac gccaggcgca catcgagact cacgccggcg agatggaaac
     2881 cgccgcgaat cactgcggcc agctgcccgg cgtacaacgg ggcacgctcg gcggtgcagc
     2941 ccggtgcaac caccaggaac tgcatctctc cgagccgggc gacggtctcg ctggcggcga
     3001 tgttctgctg cagccgccgc gccgcctcgc gcagcacctc atctccgacc tgcaagccga
     3061 gcgaggcgtt gatgtcgggc aggttgcgca gctcgatgag cagcagcgcc acgcccggct
     3121 cactcgcgcc ggcgccgatg agactctcga gcgtgcgctt ggcgaacgcc cggttcggca
     3181 gcgcagtgag cggatcgtgg taagcgtgat aggtgatgtc ggcctcgcgc gccgcgatgt
     3241 tgctctgcat ggcgttgaag gtcgacgcga ggctgcggaa ctcctcgccg cccgcgacgt
     3301 cgaccgcgat cccgtactgc ccgcgctcga tgcgctgtgc ggcgcgcacc agctcgccga
     3361 tgggacgcgt ggcgctgcga ccgagcaccg cgccgatcag cgccgcgagc agcagcgcta
     3421 cgccgtcgat gagcagcatg gcgtcgcgct gctgacggta cggggcgagc acatcctcca
     3481 gaggtttcag caggatgacc tcgaccgagt cgccgcgtgc gtccaggcgg cgcgcaaagc
     3541 tcaggtagtc cgccgctccg agccgggtga tatgcggtag ctcgctggcc gccggataga
     3601 gcgccgccgc ggcgggcggc agcgtcgagg cgatgcgcgt cacgcccgcg gcgccgtggg
     3661 tgacgatggc gacctccgag ccgaccaggt cgcggatgcg cgcggcgagg gctttatcgg
     3721 cgacaaagcc catggccacc caggcgatgg tctggggagc gcgcaccgga gccaggaaga
     3781 actgatagga gtgcgcaccg aacacgcgga agtttggctg gtcgcgcaag tcgccggcat
     3841 cctcgagcag cccggcgagc gagccctcgg catcctccgc cgcagccgcc gtcgaggcga
     3901 gcaggcggcc gtgagtatcg agcaccagca ccatgtcggc gccgatgcgt tgcgcctgat
     3961 tggctgccgc gtcgaggatg gtgggcgtgt gtccgtcggc caccgcctcc cgaaaaccga
     4021 aatcgcccgc caggactccc acaccgttcg ccagctgacc cgcgcgaaag ccgatcaggt
     4081 gctcggcgag cgttcccccc gcgcgcagct cctccgcggc ccgtgcctcg actgtccgcc
     4141 gggtgctgag cagcactgcg gcaagcgtca cagtttgggt gacgatcacc
//
LOCUS       DATDMP010000414         5126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1400615, whole genome shotgun sequence.
ACCESSION   DATDMP010000414 DATDMP010000000
VERSION     DATDMP010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400615"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VI195_07220"
     CDS             complement(<1..578)
                     /locus_tag="VI195_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6824215.1"
                     /translation="MRRFLARDYGFEARRQIIASPEGWSAPVWETFAGMGLLGLPLSA
                     DYGGFGGGAVDLMAVMEAFGEALVVEPYLSTLMGALAISRGGSRELKSTLLPAIAQGK
                     MRIAFAASDRQVIGAPSAQKLAVVKENGGDVALYVLDAGAVTLRSYRTLDGMRAADVT
                     FSKQPGERLEGGGALLEEVLDFGTALICAEAVG"
     gene            complement(633..1793)
                     /locus_tag="VI195_07225"
     CDS             complement(633..1793)
                     /locus_tag="VI195_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6824216.1"
                     /translation="MDLSYSPDELAFREEARAWLAQNLPAELRDKVAGYEELSREDLL
                     RWHRILARKGWIAPAWPKEWGGTGWNVVQRYIFEEEQGYAGAPTLPPFGISMCAPVLL
                     RFGSEAQKKRFLPRIYNGDDFWCQGYSEPGSGSDLASLKTRALRQGEHYVVSGQKIWT
                     TLAHYADWIFCLVRTDSASAKRQEGISFLLMDMKSPGISVRPLVLMDGGHEVNEVFFD
                     EVKVPVENLVHEEGKGWTVAKFLLGHERLNTGRIGASKRELAKLKEFAASRSRNGRPL
                     MEDVRFRDRLSRVEVELMALEITNLRFIDRMRRSGQPPGAEVSMLKIKGTEIQQALTE
                     LMMQAAGAEAAGSADRFAAAIGLRYCNFRKTTIYAGSNEIQRNIIAKATLGL"
     gene            complement(1793..2593)
                     /locus_tag="VI195_07230"
     CDS             complement(1793..2593)
                     /locus_tag="VI195_07230"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6824217.1"
                     /translation="MERERAPGERMSGASLFDLKGKVALVTGATRGIGKSIAEELARC
                     GAKVALCSRKSEACDTVRAEFERRGFECLAQACNVSRKDDLRALVEATLKRWGRIDIV
                     VANAAANPYFGPLAEIPDDAFDKIFANNVKSVLWLAGLTLPGMAQRGGGSFIVVGSIG
                     GLIANTVIGAYGMSKAAVHHLARNLAAEWGPKNIRVNAIAPGLIKTEFARALWEDEKR
                     RREREAATPLRRLGEPRDIGGIAVFLASDAAAFVTGQCIVADGGVTIL"
     gene            2726..3553
                     /locus_tag="VI195_07235"
     CDS             2726..3553
                     /locus_tag="VI195_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY6824218.1"
                     /translation="MGAPEPAAPLSQPAASFSYEHEGDRQFATTLARGLEVLRCFTPL
                     EPLLGNKEISVRTGLPKPTVSRLTYTLTKLGYLRHNMRLGKYQLGSAVLSIGYPLLAS
                     MNVRQVARPLMKELADYCNGSVSMGVRDRLSMVYVESCRSGNGITTLPDIGTSVPISQ
                     SVIGRAFLAACTPPEREAVLNHLKVKDPEAHRKYRASIDKSLEDIRGRGFCASMGELR
                     REVYGVGAPMRRTVDGEIVAFNCTVPAFLVKKGQLEDDLGPRLVAMVRNIEASLGLH"
     gene            3676..4488
                     /locus_tag="VI195_07240"
     CDS             3676..4488
                     /locus_tag="VI195_07240"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY6824219.1"
                     /translation="MHAGRIALVLLLAWLFEALAARLMRLFRLYMERRIAAPDEVPRI
                     ETLARVFRYAAAVLIALVAGSLVLDELGISVAPILATAGVAGVAIGFGAQSLVKDYFT
                     GFFLLLEDQIRQGDVVEVGGKSGLVEEVTLRYVRLRDFDGHVHFVPNGEIKVVTNRTR
                     GFAHAVIEIGVDYDTDVDRALAIMKDVGKELRADPVWGPKIAADPEIIGVEKLADSAV
                     VLRGRVQVVPPIEQWNVRREYLKRLKEAYGARGIGIPFPHLTLHRKPRPKAG"
     gene            complement(4451..>5126)
                     /locus_tag="VI195_07245"
     CDS             complement(4451..>5126)
                     /locus_tag="VI195_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6824220.1"
                     /translation="AFLSAQQEKILQLKPDLVIGFSDLQAEIARDLAKAGLNVLLLNQ
                     RTVQEILDMIVLLASIVGAPEKGAALVASLEKSLSDVKGKARQLKRRPRVYFEEWDEP
                     MISAIRWVSELVELAGGEDIFKEKSFSQAAGGRTVADPAEVVKRKPDVIVGSWCGRKF
                     RPEKVAARPGWERIPAVRDAQLFEVKSCEILQPGPAALTDGVRRLHAIFAKWASRPSA
                     WASDAA"
BASE COUNT          855 a         1774 c         1603 g          894 t
ORIGIN      
        1 cccaccgcct cggcgcagat cagcgcggtg ccgaagtcga gcacctcctc gagcagcgcg
       61 ccgccgcctt ccagccgctc cccgggctgc ttggaaaacg tcacgtccgc tgcccgcatt
      121 ccatccagcg tgcggtagga cctcagggtg accgcccccg catccaggac gtaaagcgca
      181 acgtcaccgc cgttttcttt caccaccgcc agcttttgcg cgctcggcgc gccgatcacc
      241 tggcgatcgg aagctgcgaa ggcaatccgc atttttccct gcgcgatcgc cggcaacagc
      301 gtgctcttca gttcgcgcga gccgccgcgc gagatggcaa gcgcgcccat gagcgtcgag
      361 agatatggct cgaccacgag cgcctcgcca aaggcctcca tgaccgccat caggtccacg
      421 gcgccgccgc cgaacccgcc gtaatccgcg gagagcggca atccgagaag ccccatgccg
      481 gcgaacgttt cccacaccgg cgcgctccag ccttccggcg aggcgatgat ctgcctgcgc
      541 gcctcgaagc cgtagtccct ggcgaggaac cggcgcacgg aatccgcgag gagctgctgc
      601 tcttcgttat agtcgaagtt catgagcccg cttcacagcc cgagggtggc cttcgcgatg
      661 atattgcgct ggatctcgtt cgagccggca taaatggtcg tcttgcggaa gttgcaatag
      721 cgcaggccga tcgccgcggc gaagcggtcg gccgagcccg ccgcttcggc accggccgcc
      781 tgcatcatga gctcggtcag cgcctgctgg atttccgtcc ctttgatctt gagcatcgac
      841 acttccgcgc cgggcggctg cccgctgcgg cgcatgcggt cgatgaagcg caggttcgtg
      901 atttcgagcg ccatcagctc aacctccacg cgcgagagcc tgtcgcggaa gcgcacatcc
      961 tccatcagcg gccggccgtt cctcgaccgg gaagcggcga actccttcag cttggcgagc
     1021 tcgcgcttgg aggcgccgat gcgcccggtg ttgaggcgct catggcccag caaaaacttt
     1081 gcgaccgtcc agcccttgcc ctcctcgtgc acgaggttct ccaccgggac cttcacctcg
     1141 tcgaagaaca cctcgttgac ctcgtgaccg ccgtccatca gcacgagcgg ccgcacgctg
     1201 atcccggggg atttcatgtc catgaggagg aacgaaatgc cttcctggcg cttcgcgctc
     1261 gcgctatccg tgcgcacgag acagaagatc cagtcggcgt aatgggcgag cgtggtccag
     1321 atcttctgtc cgctgaccac gtaatgctcg ccttgcctga gcgccctcgt cttcagcgag
     1381 gcgaggtcgc tgcccgagcc gggttccgag tagccctggc accagaaatc gtcgccgtta
     1441 tagatgcgcg gcaggaacct cttcttttgc gcctcgcttc cgaagcgcag aagcaccggg
     1501 gcgcacatcg agattccgaa cggcggcagg gtcggtgcgc ccgcgtagcc ctgctcttcc
     1561 tcgaagatgt agcgctgcac cacgttccag ccagtgccgc cccattcctt cggccatgcc
     1621 ggcgcgatcc agcctttcct ggcgaggatg cgatgccacc gaaggaggtc ctcgcgcgaa
     1681 agctcctcat agccggccac cttgtcgcgc agctccgcgg gcaggttctg cgcgagccag
     1741 gccctcgcct cttcgcggaa cgcaagctcg tcgggcgaat agctgaggtc catcagagaa
     1801 tggtaacccc gccgtcggcc acgatgcatt ggccggtgac gaaagcggcc gcgtccgacg
     1861 cgaggaatac ggcaatgccg ccgatgtcgc gcggctcgcc caggcgccgc aagggggtgg
     1921 ccgcctcgcg ctcacgcctg cgcttttcgt cctcccacaa ggcgcgagcg aattcggtct
     1981 tgatcagacc cggcgcgatg gcgttcacgc ggatgttctt cggtccccat tcggcggcga
     2041 gattgcgcgc gaggtggtgc acggcagcct tcgacatgcc gtaggcgccg atcaccgtgt
     2101 ttgcaatcag cccgccgatg gagccgacga cgatgaagct cccgccgccg cgctgcgcca
     2161 tccccggaag ggtgaggccc gcgagccaca gcacgctctt gacgttgttc gcgaagatct
     2221 tgtcgaacgc gtcgtccgga atttcggcga gcgggccgaa atagggattg gccgccgcat
     2281 tcgccaccac gatatcgatc cggccccagc gcttgagcgt cgcctcgacc agcgcgcgca
     2341 gatcgtcctt gcgtgaaacg ttgcacgcct gcgcaaggca ttcgaatccg cggcgctcga
     2401 attccgcgcg caccgtgtcg caggcttcgc tcttgcggct gcatagcgcc actttcgcgc
     2461 cgcagcgcgc gagttcttcg gcgatggact tgccgatgcc ccgcgtcgcg ccggtgacca
     2521 gcgccacttt ccccttgagg tcgaacaacg aggccccgct catccgctca cccggcgcac
     2581 gctcacgctc cattccccgc tgcggatttc actatgtgaa atatcgtacg actaagttga
     2641 catgcagacg gcgggcgaca gaatatcgaa acgcgcaaca cttcgctgca cctcaacacg
     2701 aaataagcca tcagaggagg ccaacttggg agctccagaa cccgcggcgc cgctttcgca
     2761 gcccgcagcc agcttcagct acgagcacga gggtgaccgc cagttcgcca cgacgctcgc
     2821 ccgcggactc gaggtgctgc gctgcttcac gcccctcgag ccgctgctgg gcaacaagga
     2881 aatctcggtg cgcacgggcc tgccgaaacc gacggtctcc cgcctcacct ataccctcac
     2941 caagctcggc tacctgcggc acaacatgcg cctgggcaaa tatcagctcg gctcggcggt
     3001 gctttccatc ggttatccgc tgctcgcctc catgaacgtg cgccaggtgg cgcgcccgct
     3061 gatgaaggag ctggcggact actgcaacgg ctccgtctcc atgggggtac gcgatcgcct
     3121 gagcatggtc tacgtggagt cgtgccgcag cggaaacggc atcacgacgc tgcccgacat
     3181 cggcacgtcg gttccgatct cgcaatcggt cattggccgc gcgtttctcg ccgcgtgcac
     3241 gccccccgag cgcgaggcgg tgctgaatca cctgaaggtc aaggatcccg aggcgcaccg
     3301 caagtaccgc gcttcgatcg acaagtcgct ggaggacatc cgcggccgcg gcttctgcgc
     3361 ttccatgggc gagctgcgcc gcgaagtcta cggcgtgggt gcccccatgc gccgcacggt
     3421 ggacggcgaa atcgtcgctt tcaactgcac cgtgccggcg ttcctggtga agaaaggcca
     3481 gctcgaggac gatctcgggc cgcgcctggt cgccatggtg cgcaacatcg aagcttcgct
     3541 cggattgcac tagagcggta atggcgcctc ttccctgaag taggatcggc agcttccgca
     3601 gcaccgaggg ccgggccgat gaactcgctc ttcgcctact tcaacgacct cgacgcgggc
     3661 gcgaaagtct ttgccgtgca cgccgggcgc atcgccctcg tgctgctgct cgcgtggctt
     3721 ttcgaagccc tggccgcccg gctgatgcgc ctcttccggc tgtacatgga gcgacgcatc
     3781 gccgcgcccg acgaggtgcc gcgcatcgag acgctcgccc gcgtgtttcg ctacgcggcc
     3841 gccgtgctga tcgcgctcgt cgcgggctcg ctcgtcctgg acgagctcgg catctccgtc
     3901 gcgccgatcc tcgccaccgc cggggtggcc ggcgtcgcca tcggctttgg cgcgcaaagc
     3961 ctggtgaagg actacttcac cggcttcttc ctgcttctcg aggaccagat ccggcagggc
     4021 gatgtcgtcg aggtcggggg caagagcggg ctcgtggagg aagtgacgct gcgttacgtg
     4081 aggctgcgcg acttcgacgg gcacgtccat ttcgtgccga acggcgagat caaggtcgtc
     4141 accaaccgca cgcgcgggtt cgcgcatgcg gtgatcgaga tcggggtgga ctacgacacc
     4201 gacgtggatc gcgcgctcgc gatcatgaag gacgtcggca aggagctgcg cgccgatccg
     4261 gtatggggtc ccaagatcgc cgccgaccct gaaatcatcg gcgtggagaa gctggccgat
     4321 tcagcggtgg tgctgcgcgg ccgggtgcag gtggttccgc ctatcgagca gtggaacgtg
     4381 cgccgcgaat acctcaagcg gctgaaggaa gcctacggcg cccggggaat cggaattccg
     4441 ttcccgcatc tcacgctgca tcggaagccc aggccgaagg ccggctagcc cacttcgcga
     4501 agatggcgtg cagccggcgc acgccgtcgg tgagcgcagc cgggccgggc tgcaggattt
     4561 cgcaggactt cacttcgaaa agctgcgcat cgcgcaccgc cgggatgcgc tcccagccgg
     4621 gccgcgcagc gaccttctcg ggccggaatt tcctcccgca ccaggagccg acgatcacgt
     4681 cgggctttcg ctttaccact tccgcggggt cggccacggt gcggccgccg gccgcctgcg
     4741 agaacgactt ttccttgaaa atgtcctcgc cgcccgcgag ctcgaccagc tcgctcaccc
     4801 agcggatggc gctgatcatg ggctcgtccc attcctcgaa atacacgcgc ggccggcgct
     4861 tcagctggcg cgcctttcct tttacgtcgg aaaggctttt ctccagggaa gcaacgagcg
     4921 cagcaccctt ctcgggcgct ccgacgatgg aagcgagcag gacaatcatg tcgaggattt
     4981 cctgcaccgt gcgctgattc agcagcagca cattcaggcc cgccttcgcc aggtcgcgcg
     5041 cgatctcggc ctgcagatcg gagaacccga tcacaaggtc gggctttagc tgcagaattt
     5101 tttcctgctg ggcgctgagg aaggcc
//
LOCUS       DATDMP010000415         3872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_289810, whole genome shotgun sequence.
ACCESSION   DATDMP010000415 DATDMP010000000
VERSION     DATDMP010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289810"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VI195_07250"
     CDS             <1..873
                     /locus_tag="VI195_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824221.1"
                     /translation="AHLHLGKTADWVAVTDSAVWVGTTGPEGVAQIDPRTNTVISAVA
                     LPGNPCAGLAIGFAALWVPLCAKPNALARVDLRTRLVTLVPGVGPAQREGGIATSRDS
                     VWLVIDGKATLARIDPGTLRITQRIPVPSGSLNPLYSDGQIWVTRATGAELTIINADS
                     GALIGTAHTGPLPHFLTAGAGAVWTLNQGDGTVTRIDVQSRRATLTTALRTPGHGGDI
                     AFGHGIVWTTMMKVPLSATEAETGRLLCQWTGPGGDALGLGENAIWLTSYDAGDLYRF
                     DLEDVVRRCSGSDR"
     gene            complement(878..2362)
                     /locus_tag="VI195_07255"
     CDS             complement(878..2362)
                     /locus_tag="VI195_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6824222.1"
                     /translation="MKLAPITAVLGCLVSALQAEPMKLTAEESAATRTLVGDVYAGGR
                     AFEYLEVLSDEFGPRLTGSEQYQRSVQWAAGQLREMGMTQVRLEPVVIKHGWQRGSAQ
                     ARLLGKTPRTLHVASYGWSPPTPHPALRGPIVFLEDTTDAAIAAARVQGALVLIDRAS
                     LTGPVAFRHTTPADWERERLYETLDRRLKNAGATAALVYTKTFNQVLRTSAPTEGGEA
                     LALPVASIGREDALLIRRRLAKGPVEIELSLDNLLTGPVTLENVIAELPGRERPAEWV
                     MLGAHLDSWDFATGAQDNGSGVAQVMEAARALAHLPSRPRRSLRFVLWASEEQGENGS
                     KAFVRKHAATMGQVVAYLNTDTGAGRPLGWNLAGRSDLARALAPLASLLERLGGSATS
                     DDLDFDTDVAAFIVAGVPTLNLDVVDDQYDAVVHHKPADTLDKVDIHNLTAGAAMLAV
                     TAYALAETAEPAVPHLSVSQREQLLERTGALEYVRSSSMKDLLQ"
     gene            2625..3002
                     /locus_tag="VI195_07260"
     CDS             2625..3002
                     /locus_tag="VI195_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824223.1"
                     /translation="MSDPPAIVGTWVVQAPSAPFPLHLFVFHSDGTVQQSNPDAGDPH
                     TSDSNAMGVWVGDGDRIRGKLVEITADRATHQFVSRGEIAFSLEVTGNEFRGTASANF
                     YDANGQLLRGPVQTPLAGQRILP"
     gene            complement(3018..>3872)
                     /locus_tag="VI195_07265"
     CDS             complement(3018..>3872)
                     /locus_tag="VI195_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY6824224.1"
                     /translation="AVRAMTADSGWAGDALGFPRIQGADACGHIAAVGAGVDARRLGE
                     RVLVDPVLRRASGRPLYFGSDTPGAFAEFAAVPAQNAFAVRSTLSDIELASFPCAYLA
                     AENMLTRAQVTAGETLLVAGASGGVGSAAVQLARRRNATVIALSEPAKAAALPELGAA
                     RVLPRDSDLLAALGRESIDVVIDVVGGEGFAQRLEVLVRTGRYAVAGAIAGPFVSLDL
                     RTLYLKDLCLLGCTIPGAEVFPNLIGYIERGELRPVIAATYPLSEIVAAQAEFMRKAH
                     VGKIVLTI"
BASE COUNT          680 a         1320 c         1248 g          624 t
ORIGIN      
        1 gcgcaccttc acctgggaaa gactgcggac tgggttgcag tcaccgacag cgccgtctgg
       61 gtcggtacca cggggcctga gggtgtggcg cagatcgatc cgcgcacgaa tacagtgatt
      121 tctgccgtcg ccctgcccgg caatccctgc gcgggacttg cgattggatt cgccgcgctg
      181 tgggtaccgc tgtgcgccaa gcccaacgcc ttggcgcgcg tcgacttgcg cacgcgcctg
      241 gtcaccttgg tccctggcgt gggtccagcg cagcgcgagg gtggaatcgc cacgagccgc
      301 gacagcgtgt ggctcgtgat cgacggcaaa gccacacttg cccggatcga tcccggcaca
      361 ctgcgcatca ctcagcgcat cccagttccc agcggctcgc tcaatccgct ctacagcgac
      421 gggcagatct gggtgacgcg agcgaccggt gcggagctca ccatcatcaa cgccgactcg
      481 ggcgcgctca tcggaacggc ccataccggc ccgctaccgc acttcctcac cgcgggtgca
      541 ggggcggttt ggacgctcaa tcagggcgat ggaacggtga cgcgcatcga tgtgcagtcg
      601 aggcgcgcca cgctgaccac cgcgctgcgc acgccaggcc atggcgggga catcgccttc
      661 ggccacggca tcgtatggac caccatgatg aaggtgccgc tttccgccac cgaggcagag
      721 accgggaggc tcctctgtca gtggaccggg cccggtggcg acgcactcgg cctcggtgag
      781 aacgcgatct ggcttacctc ctatgacgcc ggagacctct accgcttcga tctcgaggac
      841 gttgtgcgcc gctgcagcgg atcagaccgc tgagcgctca ctgcagcagg tctttcatcg
      901 aggagctgcg cacatattcg agcgcgccgg tgcgctcgag cagctgctcg cgttgcgaga
      961 cgctcagatg cgggaccgcc ggctccgccg tctcggccag ggcgtatgcc gtaacggcaa
     1021 gcatcgctgc gcccgcagtg agattatgga tgtctacctt gtcgagcgtg tcggccggct
     1081 tgtgatgcac gaccgcatcg tactgatcgt ccacgacgtc aagattcagc gtaggtactc
     1141 cggccacgat gaaggccgcc acatcggtat cgaagtcgag atcatccgag gtggcactgc
     1201 cgccgaggcg ctcgagcagc gatgcgagcg gcgccagcgc gcgcgccagg tcgctgcggc
     1261 ccgccaggtt ccagccgagt ggtcgtccgg ctccggtatc ggtgttgaga taggcaacca
     1321 cctggcccat ggtggcggcg tgcttgcgca cgaaagcctt cgagccgttc tcaccctgct
     1381 cttccgaggc ccacaacacg aaacgcagtg agcgacgcgg gcgcgacggc aggtgggcca
     1441 gcgcgcgagc ggcctccatg acctgtgcga caccgctgcc gttgtcctgt gcgccggtgg
     1501 cgaagtccca ggagtcgagg tgcgctccca gcatcaccca ttccgccggg cgctcgcgcc
     1561 cggggagctc ggcgatgaca ttctcgagag tgaccggacc tgtgagcaga ttatcgagcg
     1621 acagctcgat ttccacgggc ccctttgcca gccggcgccg gatgagcagc gcgtcttccc
     1681 ggccaatcga ggcgaccggc aatgcgagtg cttcgccccc ttccgtgggc gcgctggtcc
     1741 gcagcacctg attgaacgtc ttggtgtata ccagcgccgc cgttgccccg gcgtttttca
     1801 gacgccgatc cagagtctcg tagagtcgct cgcgttccca gtccgcggga gtggtgtggc
     1861 gaaacgcgac cggcccagtg aggctcgcac ggtcgatcaa caccagggcg ccctggactc
     1921 gcgcggcggc gatcgccgcg tcagtcgtat cctcgagaaa gacgatcggc ccgcgcagcg
     1981 ccggatgcgg cgtcggcggt gaccagccgt aggaggccac gtgcagcgtg cgcggcgtct
     2041 tgccgagcag ccttgcctgc gcggacccgc gctgccaccc atgcttgatg acgaccggct
     2101 cgagacggac ctgtgtcatt cccatctcgc gcaactgccc cgcggcccat tgcaccgagc
     2161 gctgatattg ctcggaaccc gtgagtcggg gcccgaactc atccgacagc acctcgaggt
     2221 actcgaaagc gcgtccgccg gcataaacgt cgccgacgag cgtgcgcgtc gcggcggatt
     2281 cctccgccgt gagcttcatc ggctcggcct ggagagctga caccagacac ccgagcacag
     2341 cggtaatcgg agcgagcttc atcgatgtta cttccggcag cgtgcaacac gagatcgcga
     2401 gatggccatt atccgagatg catgaatcgg ccgtccacgc ccctgccagc gtgtagcccg
     2461 aagagttgca atacggtaga tttcgccaac ccgagagctc gagccactga cgatgggcgg
     2521 cgcggcttgc ggagcagcaa ctggtgcaga agaaatctct tgcagctctc tacttgctcg
     2581 ccgtgtggct cggcactcca ggttgccatc aggcttcgag aaatatgagc gacccgcccg
     2641 cgatcgtggg tacttgggtc gtgcaggcgc cgagcgcccc gtttccgctc cacctgttcg
     2701 tgttccactc cgacgggacc gtgcagcaat ccaatcccga cgccggagat ccgcatacca
     2761 gtgacagcaa tgccatgggc gtgtgggtgg gcgacggcga ccgaatcagg ggaaagctcg
     2821 ttgagatcac cgccgaccgg gcaacgcacc agttcgtcag ccgcggagag attgcctttt
     2881 cgctcgaggt aacgggcaac gagtttcgcg gcacggcgag tgcgaacttc tacgacgcga
     2941 acgggcaact gttgcgcggg cccgtgcaaa ccccattggc cggtcagcgc atcctgccat
     3001 gaataccgcc gcgcgggtca gatcgtcagc acgatcttgc cgacgtgggc tttcctcatg
     3061 aattcggcct gcgccgcgac gatctcgctc aacggataag tggcagcgat caccggacgg
     3121 agctcgccac gctcgatgta gccgatgaga ttcggaaaga cctccgcgcc cggtatcgtg
     3181 cagccaagca aacatagatc cttcaggtaa agcgtgcgca gatcgaggct cacgaacggc
     3241 ccggctatcg cgcccgccac cgcgtagcgg cccgtacgca ccaaaacctc caggcgctgc
     3301 gcaaagccct cgccaccgac gacgtcgatc accacatcga tcgactcgcg gccgagcgcc
     3361 gccaacagat cggaatcgcg aggcagcacg cgcgcggcac cgagctccgg gagcgccgct
     3421 gccttcgctg gctcagacag cgcgatgacg gttgcgttgc gccgcctggc gagctgcacc
     3481 gcagccgacc cgacgccgcc cgatgcgccg gccaccaaaa gcgtctcccc ggcggtcacc
     3541 tgtgctcggg tgagcatgtt ttcggcggcg aggtacgcgc agggaaaaga ggccagctcg
     3601 atgtcggaaa gcgtgctgcg caccgcaaag gcgttctgtg ccggaacggc agcaaattcc
     3661 gcgaacgcac ctggcgtatc cgagccgaaa taaagcggcc gcccggatgc ccgccgtaac
     3721 accgggtcga ccagcactcg ttcgccgagt cggcgcgcgt ccacgccggc gcccaccgct
     3781 gcgatgtggc cacacgcgtc ggctccttgt atgcgcggga agccgagcgc atcacccgcc
     3841 cagccggagt ccgcggtcat cgcccgcacc gc
//
LOCUS       DATDMP010000416         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_734514, whole genome shotgun sequence.
ACCESSION   DATDMP010000416 DATDMP010000000
VERSION     DATDMP010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734514"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2162)
                     /locus_tag="VI195_07270"
     CDS             complement(<1..2162)
                     /locus_tag="VI195_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6824225.1"
                     /translation="MRRRSSGLLSWLTLVAVSAQAQSVGETPLGSAVERQLSVEQPAA
                     WARDPEQIKTEAGDRLEPRQVLTESFETVKLKNVIPPIHFESGVAKIPDNYVETLRKA
                     LYALRERRNVRLHLVGHADTQRLSPALARTFGDNAGLSRERAGEVAEFLKRALQLKPE
                     AIAYEWAGDTHPIASNDTAEGRALNRRVEVEVWYDQPKAREADDELLVRQEIKQVKVC
                     RVQTLCKLRFNEGAARRSRLSNVVPPLHYDAENAQVTPEFIEHIRKALNNLHDKPNVV
                     IKFIGYTDQVALSERDERIYGDLVALSKARAHRVALAVQEALKLPGTAVASDGRGAAA
                     PLGANDTPQGRALNRRIEVQFWYDDPLQELPDEPQMCPNPGTEVVTKVYDPPWGSIAP
                     LQLINGRAIIPPGYTESLRRAMTDVHDKSNVRLRFIGYTGNERLDRRTAAVYGDDVGL
                     SAARARRAMEVVSEQMHLSASQAEHEGRGFVQSSDVVNVGFTQGETSYVVVQVVYDEP
                     AVLDDYEGVKITPLTHELTPQNPLGVNTMHITVDGVPIDDPGRSSADVQRCTDVALDK
                     ADIRFQFDDLKARRRLSVAVAPSVVVLQKTPDEGFIGTPVRFQMYTNYAAFIARAEVR
                     VFEAGRSTQSTPLAVVPIDPNGSAEWQPRARQLSAAGQKLQYLVRAYGKDGQFDDTKP
                     LSLWMIHDGSRAPEEPNTAPPSGGDYQVTDDSGATTAAP"
     gene            complement(2168..>3333)
                     /locus_tag="VI195_07275"
     CDS             complement(2168..>3333)
                     /locus_tag="VI195_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824226.1"
                     /translation="TWDNPAQNASASVSIQLDTPSGALTVSKTTPLLDVSRGQLVPYT
                     ITVTNVLTGTVSGVALVDSFPAGFRYIQGSARIDDVPAEPTVSGFQLTWSNLSFAGGA
                     HHTILVLLAVGAGVGEGEFVNRAQAFAEVTGAAVSRAKAPAALTRNSVKSVQAPAAVL
                     GNAVSAEATAKVRVVPDTTFDCTDVIGKVFDDANGNGQQDPGEKGLPGVRVLTTRGLA
                     AITDEFGRFHITCAITPLEGRGSNFALKLDDRTLPSGYRPTTPPVLVERATRGKTLRF
                     EFGASIHRVVSLDLTDAVFEPGTTDMRLQWRPRLDLLLTELRKSRSVLHLSYLGDVED
                     RQLVDRRLEAIAKSISDAWRAMNCCYELRIEREVYWLRGSPPREPVERLRGAQ"
BASE COUNT          517 a         1106 c         1095 g          615 t
ORIGIN      
        1 ggcgccgccg tcgtggcgcc gctgtcatcg gttacctggt agtcgccacc gctcggcggc
       61 gcggtgttcg gctcctcggg cgcgcgggat ccatcgtgaa tcatccacag ggagagcggc
      121 ttcgtgtcgt cgaactggcc atccttgcca taggcgcgga ccaggtactg taacttctgc
      181 cccgcagcgg acagctgccg cgcgcgcggc tgccactcag ccgagccgtt cggatcgatc
      241 gggacgacag ccagtggcgt gctctgcgta gaacgccccg cttcgaatac ccgcacctcc
      301 gcccgcgcga tgaacgccgc gtagttggtg tacatctgaa aacgcacggg cgtcccaatg
      361 aacccctcgt ccggagtttt ctgcaacacg accacgctcg gggcgactgc cacactcagc
      421 cggcgtcgcg ccttgagatc atcgaactga aaccgaatgt ctgccttatc cagagccacg
      481 tccgtgcagc gctgcacatc ggctgagctc ctgccggggt cgtcgatggg cacgccatcc
      541 acggtgatgt gcatcgtgtt gacgcccagc gggttctgtg gtgtcagctc gtgcgtcagg
      601 ggcgtgatct tcacgccctc gtagtcatcg agtacggcgg gctcgtcgta gacgacctgg
      661 accacgacgt acgaggtctc tccctgcgtg aagcccacat tgaccacatc gctcgactgc
      721 acgaagcccc ggccttcgtg ctcggcctgc gaggcggaaa gatgcatctg ctcggaaacg
      781 acttccatcg cccgacgcgc ccgcgcggcg gaaagtccga cgtcgtcgcc atacaccgcg
      841 gcggtgcggc gatcgagccg ctcgttgccc gtatacccga tgaagcgcaa gcgtacgttg
      901 gacttgtcgt gaacgtcggt catggcgcgc cgcaggctct cggtgtagcc gggcggaatg
      961 atcgcgcgcc cgttgatgag ctgcagaggt gcaatgctgc cccagggtgg gtcgtacacc
     1021 ttcgtgacca cttcggtgcc cggattcggg cacatctggg gctcatcggg cagttcctgc
     1081 agcgggtcgt cgtaccagaa ctgcacttcg atgcggcggt tcagcgcgcg cccctgcggg
     1141 gtgtcattcg cgccgagcgg cgccgcggca ccccggccgt cgctcgcgac tgcggtgccc
     1201 gggagcttca gcgcttcctg caccgcgagc gccacgcggt gtgcgcgtgc cttcgagagc
     1261 gcgacgagat cgccgtaaat gcgctcatca cgctccgaca gcgcaacctg gtccgtgtag
     1321 ccgatgaact tgatcaccac gttcggcttg tcgtgcaggt tgttcagtgc ctttctgatg
     1381 tgctcgatga actccggggt gacctgtgcg ttctccgcgt cgtagtgcaa tggcggaacg
     1441 acattgctca aacgtgaccg ccgcgccgcg ccttcgttga agcgcagctt gcagagcgtc
     1501 tgcacccggc agaccttgac ctgcttgatc tcctgtcgca cgagcaattc atcgtccgct
     1561 tcgcgcgcct tcggctggtc gtaccagact tccacctcca cgcggcgatt gagtgcccgg
     1621 ccctcagcgg tgtcgttcga ggcaatgggg tgtgtgtcgc cggcccactc gtacgcaatg
     1681 gcttccggct tcaattgcag tgcccgcttg aggaattccg ccacttcgcc cgcgcgctcg
     1741 cgtgagagtc cggcgttatc cccgaaagtg cgggcgagcg ccggagagag ccgctgagtg
     1801 tcggcgtgac cgaccaggtg cagccgcacg ttgcgtcgct cgcgcagcgc atagagcgcc
     1861 ttgcgcagcg tctccacgta attgtcggga atcttcgcca cgccggattc gaagtgaatg
     1921 ggcggaatga cgttcttgag cttcacggtc tcgaagctct cggtgagcac ctgccgcggc
     1981 tcgagccggt cgcccgcctc ggtcttgatc tgttcggggt cccgcgccca tgccgccggc
     2041 tgctccaccg acaattgccg ctcgaccgcg ctgccgagcg gcgtctcgcc aacgctctgc
     2101 gcctgtgcgc tcacggcgac gagcgtgagc caactgagga gcccggagct gcgcctgcgc
     2161 atgccgctca ctgggcacct cgcagtcgct cgacgggttc ccggggtggg ctgccgcgca
     2221 gccagtagac ctcgcgctcg atcctgagct cgtagcagca gttcatcgcc cgccaggcgt
     2281 cactgatcga cttagcgatc gcttcgagac gccggtcgac cagttgcctg tcctcgacgt
     2341 cgccgagata ggacagatgc aggaccgatc gggacttgcg cagctctgtc agcagcaggt
     2401 ccaatctcgg gcgccattgc aggcgcatgt cggtggttcc cggctcgaac accgcatccg
     2461 tgagatcgag actcacgacg cgatggatcg aggcaccgaa ctcgaagcgc agggtcttgc
     2521 cgcgtgtcgc gcgttccacg agcacgggcg gtgtcgtggg tcgatagccg ctcggcagcg
     2581 tccggtcatc gagcttgagc gcgaagttgc tgccccgtcc ctcgagcggc gtgatcgcgc
     2641 aggtgatgtg gaatcgcccg aactcgtccg tgatggcggc gagaccgcgg gtggtgagga
     2701 cccgcacccc gggcagaccc ttctcccctg gatcctgctg gccgttaccg ttggcgtcgt
     2761 cgaagacctt gccgatgacg tccgtgcaat cgaaggtcgt atccggcacc acgcggacct
     2821 tggcggtcgc ctcagcggac accgcattgc cgaggaccgc ggcgggtgcc tggacgctct
     2881 tcacggagtt gcgcgtgagc gcggcgggag ccttggcgcg gctcacggca gcgccggtga
     2941 cctcggcgaa agcctgggcg cggttcacga actcgccttc gcctacgcct gcacccacag
     3001 ccagcagcac caggatggtg tgatgcgcac cgcctgcgaa gctgagattg ctccaggtga
     3061 gctggaaccc ggacacggta ggttccgccg gcacgtcgtc gatgcgcgct gagccctgga
     3121 tgtagcggaa tccggcgggg aagctatcta cgagcgctac gccggagaca gtcccggtga
     3181 gcacgttggt gacggtgatg gtgtacggca ccaattgccc gcgactcaca tcgaggagcg
     3241 gggtcgtctt actgacggtc agtgccccgg acggagtgtc cagctggatc gagacgctcg
     3301 ccgaggcgtt ctgtgccggg ttgtcccagg tga
//
LOCUS       DATDMP010000417         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1290095, whole genome shotgun sequence.
ACCESSION   DATDMP010000417 DATDMP010000000
VERSION     DATDMP010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290095"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..602)
                     /gene="yjgA"
                     /locus_tag="VI195_07280"
     CDS             complement(69..602)
                     /gene="yjgA"
                     /locus_tag="VI195_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY6824227.1"
                     /translation="MTARGDADQEDDGGAERPSKSARKRAAHAAQDLGEALLELPDAE
                     LAALALPEALLEAIRAGRRIASRAAGARQRQYIGKLMREVDPQPIRAALAARSARAAR
                     ATELFRRIENWRERLITEGDTALAELAQWHPAIDARAWRQRIDAARAERQRTAAGNAA
                     ARELFRALRALFDTMER"
     gene            702..2045
                     /gene="pmbA"
                     /locus_tag="VI195_07285"
     CDS             702..2045
                     /gene="pmbA"
                     /locus_tag="VI195_07285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEY6824228.1"
                     /translation="MPASDDSAIRSLGDVATLALEEARRLGASQCETDASVSQGLSVS
                     VRLGEVDTIEYQRDRGLGVSVYFGRRKGGASTADLTPAAVRETVAKACAFARYTAEDP
                     YAGLVEPEALAREIPDLDLDHPWSLTPEAAIELARACEGEGLALDARLSNSEGGAVSS
                     QRHTGVYGNSLGFLAGFSSTSHSVSCTLIAQQGDDMQRDYWWSITRDPAELEAAASIG
                     RTAGERALARLGARRLTTRRAPVAYSPEMARGLFRHFIGAVSGPSQYRKASFLLNAAG
                     EQIFPDFIGMHERPHIPKALASSPFDQEGAATHDRELVRAGVLAGYVLGSYSARRLGL
                     KTTGNAGGIHNLLVSAAPGGLERPEFLRRLGTGLLVTELMGQGVNGVTGDYSRGASGF
                     WVENGVIAYPVHEITIAGNLRSMYRDIAALGSDVDARGGIRTGSVLIGEMTIAGE"
BASE COUNT          329 a          777 c          729 g          340 t
ORIGIN      
        1 catctccgcc gcggcacgca tcgtctccca gtcggatgcg gagcccatga tgatgcctac
       61 cagcggtttc acctctccat tgtatcgaac aatgcgcgca gggcgcgaaa cagctcgcgc
      121 gctgcagcat tgcccgccgc ggtgcgttga cgctccgcgc gggcggcgtc gatgcgttgg
      181 cgccacgcgc gcgcatcgat tgccggatgc cactgcgcca gctccgcgag tgcggtgtcg
      241 ccctcggtga tgagccgctc gcgccagttc tcgatgcgac ggaacaactc ggtggcgcgc
      301 gccgcgcgcg cactgcgcgc cgcgagtgct gcgcggatcg gctgcgggtc gacctcacgc
      361 atcagcttgc cgatgtattg ccgctgacgg gcgccggccg cgcggctcgc gatacggcgc
      421 ccggcacgga tcgcctcgag cagcgcctcc ggcagcgcca gtgcggccag ctcggcatcg
      481 ggaagctcca gcagcgcttc gccgagatcc tgggcggcgt gcgcggcgcg cttgcgcgcg
      541 ctcttgctcg gacgctctgc gcccccatca tcttcctgat cagcatcccc tcgggcggtc
      601 acctgcactg cctccgcgcc gcctgcgggc aatgcggcgg cggctgctag aatttaagcg
      661 caataatgat gagctcaagc cctacacgag aaccaacgcc aatgccagcc agcgacgact
      721 ctgcgatacg ctccctcggc gatgtggcga cgttggcact cgaggaagcc cggcgcctcg
      781 gcgcatcgca gtgcgagacc gacgcctccg tgagccaggg gctgtcggtg agcgtgcgac
      841 tcggcgaggt cgacaccatc gaataccagc gcgaccgcgg cctcggggta agcgtctact
      901 tcggccgccg caagggcggg gccagcaccg ccgacctcac tcctgccgcc gtgcgcgaga
      961 ccgtcgccaa ggcctgtgcc ttcgcgcgct acaccgccga agatccctac gccggcctgg
     1021 tcgagcccga agcgctggcg cgcgagatcc cggatctcga tctcgatcac ccctggtccc
     1081 tcacccccga agcggccatc gagctggcgc gcgcctgcga aggggagggg ctcgccctcg
     1141 atgcgcgcct cagcaactcc gaaggcggcg cagtcagcag tcagcgtcac accggcgtct
     1201 atggcaactc cctgggattt ctcgccggct tctcctccac cagtcattcg gtgagctgca
     1261 cgctcattgc ccagcagggc gatgacatgc agcgcgatta ctggtggagc atcacgcgcg
     1321 atcccgccga gctcgaggcc gccgcctcga tcggccgcac cgcgggcgag cgcgctctcg
     1381 cacggcttgg cgcgcggcgg ctcacgacgc gccgcgcgcc ggtcgcgtat tcgccggaga
     1441 tggcacgtgg gctgttccgg cacttcatcg gtgccgtcag cgggccgagt caataccgca
     1501 aagcctcgtt cctgctgaac gccgcgggtg agcagatatt tccggatttc atcggcatgc
     1561 acgagcgccc gcacattccc aaggcgctcg ccagcagccc gttcgatcag gagggggcgg
     1621 ccacgcacga tcgtgagctc gtgcgcgcgg gcgtactcgc aggttacgtg ctcggcagct
     1681 actccgcgcg ccggctcggg ctgaagacca ccggcaacgc cggaggaatt cacaacctgc
     1741 tggtgagtgc cgcgccgggc ggcctcgagc ggccggagtt cctgcggcgc ctcggcaccg
     1801 ggctgctcgt gaccgagctc atggggcagg gtgtcaacgg cgtgaccgga gattactcgc
     1861 gtggcgcgag tggattctgg gtggagaacg gcgtcatcgc ctatcccgtg cacgagatca
     1921 ccatcgcggg gaatctcaga tcgatgtatc gcgacatcgc agctctcggc agcgatgtcg
     1981 atgcgcgcgg cggcatccgc accggctcgg tgctcatcgg cgagatgacc atcgcagggg
     2041 agtagcgctg cggttgctcc tcaacgcgag gaggactgcc gcgggctgat gagcgccgga
     2101 cgcggcacct cgtcgaccag atcacgcagc gtctgcgccc cgcagcggct gcggatcgct
     2161 tcctcgacgc tcgcc
//
LOCUS       DATDMP010000418         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_178835, whole genome shotgun sequence.
ACCESSION   DATDMP010000418 DATDMP010000000
VERSION     DATDMP010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178835"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..898
                     /locus_tag="VI195_07290"
     CDS             209..898
                     /locus_tag="VI195_07290"
                     /inference="COORDINATES: protein motif:HMM:NF004836.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824229.1"
                     /translation="MRIGVMGERDGSFLPHYALDAAVEHSAAHLSLPVELCWLATPRI
                     AADPESALQGCSGLWAAPGTLTSIDGALAGIRFARERGVAFFGTCGGFQHAVLEFARH
                     VLRYADAAHEAYQPSAARLFVTALACSIKGQAMPVHILPGSRAHTAYGSKDAIEEYYC
                     GHGINPPYQRLLQQHGLKITGRDASGEPRILEIAAHPFYVATLFVPQTRSTENAPHPL
                     ITEFLRAAARR"
     gene            1006..2286
                     /locus_tag="VI195_07295"
     CDS             1006..2286
                     /locus_tag="VI195_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6824230.1"
                     /translation="MPARSIVAALLSLLTGLLSQASAEEQPIAIRAGQLIDTVAGRTA
                     GAQVILVRAGIIEAVGPNVAVPPGAPVIDLAAYTVLPGLIDAHTHLTIDQVNQDPLAE
                     LEHTAAERAFGSIPNARAVLLAGFTTVRDLGSYRALVDVALRDAINRGDVIGPRMYVA
                     GAYITIAGGAGAVTGFAPDVTLPWDLRYGVANSPSEVRERVRLLAGQRVDVIKMFATG
                     AVLTHNSSPAGREATPEELAAGADEARNFGLKLAVHAHGAEGIKAAIRAGAASIEHGT
                     LMDDEGRALMKQHGTYLIPTLEVRECVGKNYPPEFVSKAQQIMTAQLGNFRKAVNAGV
                     KIGFGTDIGVCRFGTNAREFTLMVENGMTAMQAIQAATTADAELLGVSQKLGSISRGK
                     LADIIAVRGDPLQNVRLLEDVRFVMKEGRIYKQD"
     gene            2365..>2713
                     /locus_tag="VI195_07300"
     CDS             2365..>2713
                     /locus_tag="VI195_07300"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6824231.1"
                     /translation="MAMRPPVPPFTPETATQKVRLAEDAWNTRDPERVVQVYTEDTQW
                     RNRAEFPVGREQVRAFLQRKWARELDYRLIKELWTCAGNRIAVRFAYEWHDDSGSWFR
                     SYGNENWEFNAEGL"
BASE COUNT          482 a          889 c          902 g          440 t
ORIGIN      
        1 agttcctgga tcgaatgctg cgttgctttc gggtcagcgc cggtgagcgc gagcgcgccg
       61 gcgagcgcca atgcaacagc catcagcggc agccggcgca acgatgggca gcgtccatcc
      121 ttgggattgt acacaggcgc tgcggcgcgg gtttctgcgg gagtggcagt gcttgtacca
      181 tgcaggcgag cgcgcgagag gtatcctgat gagaattgga gtcatgggag agcgtgacgg
      241 cagcttcctg ccgcactacg cgctcgatgc cgccgttgag cattctgcag cgcacctgtc
      301 gctgccggtc gagctctgct ggttggcgac acctcggatc gcagcggacc ccgagagcgc
      361 actgcagggc tgctcgggct tgtgggcggc tcccggcaca ctgacgagca tcgacggagc
      421 gctcgctggc atccggttcg cgcgcgagag gggcgttgcg ttcttcggca cctgcggcgg
      481 ctttcagcac gcggtgctcg aattcgcgcg gcatgtgctg aggtatgccg atgcggcaca
      541 tgaggcctac cagccatccg ctgcgcggct attcgtcacc gcgctcgcat gctcgatcaa
      601 agggcaggca atgcccgtgc acatacttcc cggatcacgg gcgcacacgg cttacggctc
      661 gaaggacgcc atcgaggagt actactgcgg ccacggcatc aatccgccgt accagaggct
      721 gctgcagcag catggcttga agattaccgg ccgcgatgcg agcggtgagc cgcgcatact
      781 cgagatcgcc gcgcaccctt tctacgtggc gaccttgttc gtaccccaga cacgctcgac
      841 cgagaacgct ccgcacccgc tgatcactga attcctcagg gctgccgcgc ggcgctgact
      901 tcgcaccggc gcatgcgcat gcgcatgcgg cagccgcagc ccatcggtat actcggcggc
      961 cccacagcgc acatccgaaa acaacagccg ggaggggcgc cgcgaatgcc agcaaggtcc
     1021 atcgtagctg cgctgctgag tcttctcaca gggctcctga gtcaggcaag cgcggaggag
     1081 cagccgatcg ccatccgcgc cggacagctc atcgataccg tcgccggacg cacggcgggc
     1141 gcacaagtga tcctggtgcg cgccggaatc atcgaggcgg tcggacccaa cgtggcggtg
     1201 ccccccggcg cgccggtcat cgacctcgcg gcctacacgg tgttgccggg cctgatcgat
     1261 gctcatacgc atctcaccat cgaccaggta aaccaggacc cactagcgga gctcgagcac
     1321 accgcggccg agcgcgcctt tggctccata ccgaacgcgc gcgcagtgct gctcgccgga
     1381 ttcaccacgg tgcgcgatct gggctcctac cgggcgctgg tcgatgtcgc gctgcgcgat
     1441 gcgatcaatc gtggcgacgt tatcggcccg cggatgtacg tcgcgggtgc ctacatcact
     1501 atcgccgggg gcgcgggcgc ggtgaccggc ttcgctcccg atgtcacgct gccctgggat
     1561 ctgcgctatg gcgtcgccaa ctcaccgagc gaggtacgcg agcgggtgcg gctgctcgcc
     1621 ggtcagcgcg tcgatgtgat caagatgttc gcgaccggcg cggtgctcac gcacaacagt
     1681 agtccggccg gccgcgaggc gacgccggag gaactcgccg ccggcgcgga tgaggcgcgc
     1741 aacttcgggc tcaagctcgc cgtgcacgcg cacggggccg aaggcatcaa ggccgccatt
     1801 cgcgccggcg ccgcctcgat cgagcacggc acactcatgg atgacgaggg ccgcgcgctc
     1861 atgaagcagc acggcaccta tctcatcccc accctggagg tgcgcgaatg cgtcggcaag
     1921 aactacccgc cggagttcgt gagcaaggcg cagcagatca tgaccgcgca gctcggcaac
     1981 ttccgcaagg ccgtcaatgc cggcgtaaag atcggcttcg gtaccgacat cggcgtgtgt
     2041 cgcttcggca ccaatgcgcg cgagttcact ctcatggtgg agaatggcat gactgccatg
     2101 caggcgatcc aggccgcgac cacggccgat gcggagctgc tcggcgtgtc gcagaagctg
     2161 ggctcgatca gccgcggtaa gctggccgac atcattgccg tgcgcggcga tccgctgcag
     2221 aacgtgcggc tcctggaaga cgtgcgcttc gtgatgaagg aagggcgcat ctacaagcag
     2281 gactgatgat gtccgcgccg cgggtggaaa cttctccaga cccggctgtc tacatcggcg
     2341 atacattcat ccaaggagac ggcgatggcg atgcgtccac cggtacctcc cttcacgccc
     2401 gaaaccgcca cgcagaaggt tcgcctggcg gaggatgcct ggaacacacg cgaccccgag
     2461 cgcgtggtgc aggtctatac cgaagacacg cagtggcgca atcgcgccga gttcccggtg
     2521 ggtcgcgagc aggtgcgcgc ctttttgcag cgcaaatggg cgcgcgagct cgactaccgc
     2581 ctcatcaagg agctctggac ctgcgccggc aatcgcattg cggtgcgctt cgcctacgag
     2641 tggcacgatg attcaggcag ctggttccgc agttacggca acgagaactg ggagttcaac
     2701 gcagagggtc tga
//
LOCUS       DATDMP010000419         3427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_511770, whole genome shotgun sequence.
ACCESSION   DATDMP010000419 DATDMP010000000
VERSION     DATDMP010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511770"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VI195_07305"
     CDS             <1..583
                     /locus_tag="VI195_07305"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY6824232.1"
                     /translation="DITALHLWLQAYGRISIHGPVLTQLSRLAPATCTRLFELLESPR
                     PAAPLSGDATYVGGSAQGPLLGGNLSVVTRLLGTPFMPPLDGAILLLEDQGERPYRLD
                     RMWTHLRLAGVFERVRGIVLGSFTGCEEPGASYTSAEVLRELAQSEGLPCAAGFPVGH
                     GDVNEPVPLGVHVRLEADSARLIFLEPAARAAG"
     gene            complement(588..2360)
                     /locus_tag="VI195_07310"
     CDS             complement(588..2360)
                     /locus_tag="VI195_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY6824233.1"
                     /translation="MYQGEPGLLVRAFAALSLALSACGSPSAPAPKPVAGVAPAAPSR
                     DRWSEFADRFIEGYFRANPFFAVQAGRHEFDGRMSDWSGAAIAAEVARLHALRTEAQA
                     FDAGTLSESAQLEREIVLTVIDGNLFWLERAQSPFTNPAWYLEQLDPDVYLNRDYAPL
                     ATRMRGYIGYLRALPKLAAEIRANLRTPLPPSFAQRGIDDFGGYVEFFRKDGPKVFAA
                     VQDRAAQQDLAEASAAAADAMAALRQWFQRERARATGRFALGEPLFLEMLRATERVEV
                     PVAQLLEVGRADLERNTQALTAACAEYLPGGTLRACVERMRADKPSGGAVEGARIQLA
                     QLREFIVARKIVTIPSDERPQVAEAPAYNRSNVAYINVPGPYEKARGYTYEIAPPDPA
                     WSAKERAEYIPGRASLLYTSVHEVWPGHFLQFLYSNRNPSKIAALWVGYAYAEGWAHY
                     CEEMMWEEGLGNGDAEQHVGQLTDALLRNVRYLSAVGLHTQGMTLAESDRMFRERAYT
                     DPGNARQQAARGTFDPEYLKYTLGKLMIRKLRADWVARQAGTAANTDPRQYWQAFHDR
                     FLSYGGPPIPLLRKAMVGEGGSLF"
     gene            complement(2380..2697)
                     /locus_tag="VI195_07315"
     CDS             complement(2380..2697)
                     /locus_tag="VI195_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEY6824234.1"
                     /translation="MRRRRRSEVERLRRRISRLDGRSIDELYGLEPVWEPGSAPPGLP
                     PEEFVAVRCPYCGERLHTRVDLTADEPGFVEDCEVCCRPIEFDIERDAAGGLVALQVR
                     RLD"
     gene            2771..>3427
                     /locus_tag="VI195_07320"
     CDS             2771..>3427
                     /locus_tag="VI195_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824235.1"
                     /translation="MARGTLVLVCVSLLAGCAGSSLLEDPPPGVNLAGAWRLDHGASD
                     DPQKVLEQMRAQAMKIIGRQSQQQVVMPARGGRQGQAQSPESGPDPALAQPPPPGTHF
                     DPLRRSPMAHVILNMVARGDFLTVRQGPGEFVLDYGSSRRTFTPGQHSVVSAEGGVGD
                     QTSGWKGRAYVIEVKPQNGPVMTEQYELSPDGKQLVTKLHIGSSELPAVNLTRVYRPA
                     D"
BASE COUNT          546 a         1180 c         1151 g          550 t
ORIGIN      
        1 cgacatcacc gcgctgcact tatggctgca ggcctacggt cgcatcagca tccatgggcc
       61 ggtgctcacg cagctttcac gcctcgcgcc ggccacctgc acacggctgt tcgagctgct
      121 ggagtcgccg cgcccggcag caccgctctc gggcgatgcc acgtatgtcg gcggcagcgc
      181 gcagggcccg ctgctcgggg gcaacctttc ggtggtgacg cggctgctcg gcacgccgtt
      241 catgccgcca ctcgatggcg cgattctgct gctcgaggat cagggcgagc ggccgtaccg
      301 gctcgatcgc atgtggacgc acctgcgact cgcgggggtg ttcgagcgcg tgcgcggtat
      361 cgtgctgggc tccttcaccg gctgcgagga gccgggggcg agctatacca gtgccgaggt
      421 gctgcgcgag ctggcgcaga gcgaggggct gccgtgcgct gccggtttcc cggtcggcca
      481 cggcgatgtc aacgagccgg tgccgctcgg agtgcatgtg cggctcgagg ccgacagcgc
      541 cagactgatc tttctcgagc cggcggcgcg cgccgccggc tgagacctca gaagagcgaa
      601 cccccctcgc ccaccatcgc cttgcgcagc agcggaatcg gcggcccgcc gtatgacagg
      661 aacctgtcat ggaaggcctg ccagtactgg cgcggatcgg tgttggcggc agttcctgcc
      721 tggcgtgcca cccagtccgc tcgcagcttg cggatcatca gtttgccgag agtgtacttg
      781 aggtactccg ggtcgaacgt gccacgcgcg gcctgctggc gcgcattgcc ggggtcggtg
      841 taggcgcgct cgcggaacat ccgatcggat tcggcgagcg tcatgccctg ggtatgcagg
      901 ccgacggcgg agagatagcg aacgttgcgc agcagagcgt cggtcagctg accgacgtgt
      961 tgctccgcat cgccgttgcc aagtccttcc tcccacatca tctcctcgca gtaatgggcc
     1021 cagccttccg cataggcata acccacccac agcgcggcaa ttttcgaagg attgcggttc
     1081 gagtacagaa actgcaggaa atgccccggc cacacctcgt gcacgctggt gtagagcaga
     1141 ctcgccctgc cgggaatgta ttccgcccgt tccttggcgc tccacgccgg atcgggcggg
     1201 gcgatctcgt aggtgtaccc cctcgccttc tcgtaggggc cgggtacgtt gatgtaggcg
     1261 acgttggaac ggttgtaagc cggcgcctcg gcgacctggg gtcgctcatc gctcgggatg
     1321 gtgacgatct tgcgcgcgac gatgaactcg cgcagctgcg caagctggat gcgtgcgccc
     1381 tcgacggcgc cgccgctcgg cttgtcggca cgcattcgct cgacgcacgc gcgtagcgtg
     1441 ccgccaggca ggtactctgc gcaggccgcc gtgagcgcct gggtattgcg ctcgaggtcg
     1501 gcacggccca cctcgagcag ttgcgcgacc gggacctcga cgcgctcggt ggcacgcagc
     1561 atctcgagaa acagcggctc accgagcgcg aaacggccgg tcgcacgcgc ccgctcgcgc
     1621 tgaaaccact ggcgcagcgc cgccatggca tcggccgccg ccgcactcgc ctcggcgagg
     1681 tcctgctgtg ccgcgcggtc ctgcaccgct gcgaacacct tgggtccatc cttgcggaag
     1741 aattccacat atcccccgaa gtcatcgata ccacgctgcg cgaagctcgg cggcagcggc
     1801 gtgcgcaggt tggcgcggat ctccgcggcc agcttcggca gggcgcgcag gtagccgatg
     1861 tagccgcgca tgcgggtcgc gagcggcgca tagtcgcgat tcaggtacac atcgggatcg
     1921 agctgctcga gataccaggc ggggttggtg aagggagact gcgcgcgctc gagccagaac
     1981 aggttgccgt cgatgaccgt gagcacgatc tcgcgctcga gctgcgcgct ctcgctcagg
     2041 gtgccggcat cgaacgcctg cgcctcggtg cgcagcgcgt gcagccgcgc cacctccgcg
     2101 gcgattgcgg cgccgctcca gtcggacatc cgtccatcga actcatgccg cccggcctgc
     2161 accgcaaaga aggggttcgc gcgaaagtag ccctcgatga agcggtccgc aaactccgac
     2221 cagcgatccc gtgacggcgc ggccggcgcg acccctgcga ccggtttcgg cgccggggcg
     2281 gagggcgaac cacaggccga cagtgcgagg ctcagcgccg cgaatgcgcg aacgagaagt
     2341 ccgggttctc cctgatacat tgtttttctc ccgacaccct cagtccagac gccgcacctg
     2401 cagcgccacc aatccaccgg cggcgtcgcg ctcgatgtcg aactcgatgg ggcggcagca
     2461 cacctcgcag tcttcaacga agccgggctc atcggcggtc agatcgacgc gcgtatgcaa
     2521 ccgctcgccg caatacgggc agcgcacggc gacaaattcc tccggcggca gcccgggcgg
     2581 cgcgctgccc ggctcccaca ccggctcgag tccatagagc tcgtcgatgc tccgcccatc
     2641 gagacggctt atgcgccgcc gtaggcgttc aacctcactg cgtctgcgtc gtctcacatt
     2701 tgcgcccggg cgttacgaac tgcgatgctg cacgcatcgc gacaaccggc gaggatacga
     2761 caatatgccg atggctcgag gcaccctcgt gctggtgtgc gtgtccctgc tcgcgggatg
     2821 cgcgggctcg tcactcctcg aggaccctcc gcccggggtg aacctggccg gcgcctggag
     2881 gctcgatcac ggcgcgagcg atgatccaca gaaggtcctc gagcagatgc gcgcgcaggc
     2941 gatgaagatc atcggccgtc aatcgcagca acaagtcgtc atgcccgccc gcggaggtcg
     3001 gcaggggcag gcgcagagcc ccgagagcgg ccctgatccc gcgctggcac agccgccgcc
     3061 tcccggcacg cacttcgatc cgctgcggcg ctcgccgatg gcacacgtca tccttaacat
     3121 ggtggcgcgc ggggacttcc ttaccgtgcg gcaggggccc ggcgaattcg tgctcgacta
     3181 tgggtcctcc cgccgaactt tcacccccgg gcagcacagc gtggtgtctg cggagggggg
     3241 cgtgggcgac cagacctccg gctggaaggg ccgcgcgtac gtgatcgagg tgaaaccgca
     3301 gaacggcccg gtgatgaccg agcaatacga gctgtcaccc gatggcaaac agctcgtcac
     3361 gaagctccac atcgggtcgt ccgagctgcc ggcagtcaac ctgactcgcg tctaccgtcc
     3421 ggccgat
//
LOCUS       DATDMP010000420         5394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1956579, whole genome shotgun sequence.
ACCESSION   DATDMP010000420 DATDMP010000000
VERSION     DATDMP010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956579"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VI195_07325"
     CDS             <1..1767
                     /locus_tag="VI195_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY6824236.1"
                     /translation="VTHDDGTDTIGYSRLTDGNAGTFWKSNPYLTQGFTGEDDAAHPQ
                     WVILDLASKQKLNAIRIDWAAPYARDYVVQFWTGDDPIKKPAAGSWQQFAHGAIAAGH
                     GGSETLRLADLPVEAQFVRILMFHSSNTCVAGTRGDRRDCVGFAIGEISLGTLNAAGK
                     LHDLVRHTPDQDQTATYCSSIDPWHERGDLDEKAGEQVGFDRFFSSGITRGLATMVPI
                     AMLYSTPEDAVAELGYLARRGYRISYVEMGEEPDGHYTVPEDYAALYLQFAAALHRLD
                     PALKLGGPVFTGQNEDIQSWPDAHGNSSWTGRFIDYLRAHAALKELAFFSFEHYPIDP
                     GKIAWSSLYDEAHLVTHIMQVWREDGVPQEVPLFITESNISSQYSEAYMDLWGGLWLA
                     DYVGAFLTGGGNAIYYFHYLPQPFGPGHNGSPGTFNFFSADQDLHIRQPLAQYFASRL
                     INLEWLQPGNGQHRLFAATADSVDGAGHLLISAYPVLRPDGSWALLVINKDQANAHAV
                     AIRFEDAARHRMGAFTGRVSATVFGKSEYQWHPERDGGRADPDGPPTSTVIEARPATT
                     FNLPAASITVLRGSVAMTEAAR"
     gene            complement(1770..2744)
                     /locus_tag="VI195_07330"
     CDS             complement(1770..2744)
                     /locus_tag="VI195_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY6824237.1"
                     /translation="MRAPDPGLCAMLALCSLAALGAARRTSAAEPPRFTVASQGEAPL
                     VLVVYGDTRFTQRPDVVNAAARRALVERIATEKPAAILIGGDLVFEGRDPGDYQTFRT
                     ETRAWSEAHIPVYPALGNHEFRGCAADKQPCLENWWSAFDDLPLRPHRWYSVAIGSNL
                     LALVLDSGAALKPGSEQRSWFEAQMAGADAGVKFILIVLHYPPVSDPFFPRERDEKEI
                     ARYLAHHAGSLLAKVLVVGSHVHNYERYFRDEVMYVVSGGGGAKPVPAPRMFGEQSHL
                     RTGVNFHYLRLTLGKDSLAVSMVRYDATKVEGPQAWSEPDRFEIRARN"
     gene            complement(2810..3403)
                     /locus_tag="VI195_07335"
     CDS             complement(2810..3403)
                     /locus_tag="VI195_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824238.1"
                     /translation="MTALAAASMGAVSLGAQAQSAPTTQAGVASAPGKVAGAATTEVT
                     AKVVAVDPSMRTVTLQNSSGKTRTIEVGDQVRNFDQIKVGDTVHAKYTQALALELKKG
                     GGALPAPTAEGAITPAPAPGAKPGGTIAHKVTVTAEVVAVDQAKQMVTLRGPAGNEVD
                     VKAEDPSQLQNIKKGDHVQATYVEALAIKVAEASTKK"
     gene            3600..4313
                     /locus_tag="VI195_07340"
     CDS             3600..4313
                     /locus_tag="VI195_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824239.1"
                     /translation="MLQIAVINESIAIADADVQSMIAAFTSQWNKDLNSVWGVGAATF
                     TFNPKQQAPPAGAWWVVFLDNSDQANALAYHDLTNEGLPISKVFVKTIQADNASLSVG
                     ASHEICEMAVDPWLNSAYQDFQSIFWAGEICDPVEDDQYGYDIGGVLVSDFVTPDWFG
                     HKNAAGPIDLKRHATSAFEVLTGGYAQKFDPKKGWVQVTGAKAALTLRGSRAASGSRR
                     ERRTRGWKKWALSQHTFRR"
     gene            4434..>5394
                     /locus_tag="VI195_07345"
     CDS             4434..>5394
                     /locus_tag="VI195_07345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6824240.1"
                     /translation="MESTAGWTGRRSPDSPPASIAPNGQGASARLVADVEGIRALTAE
                     YEHLYRVTGNTLPFARQEWHLAWCEHLLSQTRRASQQPLFCIVRNADGTCIAIVPLIL
                     TLRRLGPLKVAALGLVGADPALTEIRNPLVAPGYERATVCAVHEQLAKVRGWDWIQWS
                     GISAALAEALKLEATPQWYQTIDDYVLDLPAAWPEMRQKFTRNLREALRHCYNSLRRA
                     GHNFEFVVARDRVEVRWALYRFLQLHTLRANMPWGPIHADRFTGRSVRAFLYDVCDRL
                     AQRDVVRVFQLRIADEVVASRIGFVVGDSLYLYYSGFDPAWGRF"
BASE COUNT          933 a         1787 c         1724 g          950 t
ORIGIN      
        1 gttacacacg atgacggcac ggacacgatc ggctactcgc gcctcaccga tggtaacgcc
       61 ggaacgttct ggaaaagcaa tccgtacctg acgcagggct tcaccggcga ggacgacgcc
      121 gcacacccgc agtgggtcat cctcgatctc gccagtaagc agaagctgaa cgccatacgc
      181 atcgactggg ccgcgcccta cgcccgcgac tacgtggtgc agttctggac cggtgacgac
      241 ccgatcaaaa agcccgccgc gggcagctgg caacaatttg cccatggagc catcgccgcc
      301 ggtcatgggg gcagcgaaac cctgcgcctc gcggatctgc cggtcgaagc gcagttcgtg
      361 cgcatcttga tgttccactc atccaacacc tgcgtcgcgg gaacgcgcgg cgaccggcgc
      421 gactgcgtgg gattcgccat cggcgagatc tcgctcggca ccctgaacgc cgccgggaaa
      481 ctgcacgatc tcgtgcgcca cacacccgac caggaccaga ccgccaccta ctgctcatcc
      541 atcgatccct ggcacgagcg cggggatctc gatgagaagg ccggcgagca ggtcggcttc
      601 gaccgctttt tctcgagcgg catcactcgc ggtcttgcca cgatggtgcc gattgcgatg
      661 ctgtactcga ctcccgagga tgccgtggcg gagctcggtt acctcgcgcg ccgcggttat
      721 cgcatctcct acgtggagat gggcgaggag cccgacgggc actacacggt gcccgaggat
      781 tatgcggcac tctatctgca gttcgccgcc gcgctgcacc ggctcgatcc cgcgctcaag
      841 ctcggcggcc ccgtgttcac cggacagaac gaggacattc agagctggcc ggatgcgcac
      901 ggcaatagct cgtggacggg acgcttcatc gactatctgc gagcgcacgc ggctctcaag
      961 gagcttgcct tcttctcctt cgagcactat ccgatcgatc cgggcaagat cgcctggagc
     1021 agtctctacg acgaggcgca cctggtgact cacatcatgc aggtgtggcg cgaggacggg
     1081 gtgccgcagg aggtgccgct gttcatcacc gaatccaaca tctcctcgca gtactcggag
     1141 gcctatatgg atctctgggg cggactgtgg ctcgccgact atgtcggtgc gttcctgacg
     1201 ggcggtggca acgcgatcta ttactttcac tacctgcccc agcccttcgg cccggggcac
     1261 aacggctcac cggggacgtt caacttcttt tccgccgacc aggacctgca catccggcaa
     1321 ccgctcgcac agtactttgc cagccggctg atcaatctcg agtggctgca gccgggcaat
     1381 ggacagcacc ggctgttcgc cgcgacggcg gatagcgtcg atggcgccgg acacttgctg
     1441 atcagcgcct atccggtgct gcgacccgat ggcagctggg cgctgctcgt catcaacaag
     1501 gaccaggcga acgcgcacgc agtggccatc cgtttcgagg atgctgcgcg ccatcgcatg
     1561 ggtgccttta ccggccgggt gagcgccacg gtgttcggca aatcggagta tcagtggcac
     1621 ccggagcgcg acggcggccg ggccgatccc gacggaccgc cgacgagcac ggtcatcgag
     1681 gctcgaccgg ctacgacctt caacctgccg gctgcctcga tcacggtgct gcgtggcagc
     1741 gtcgccatga cggaagccgc tcgctgagat cagttcctcg cgcggatctc gaatcgatcc
     1801 ggctccgacc aggcctgcgg gccctccacc ttggtggcat cgtagcggac catggacacc
     1861 gcgaggctgt ccttgcccag cgtgagccgc aggtaatgga agttgacccc ggtgcgcagg
     1921 tgcgactgct cgccgaacat gcgcggcgcc ggcaccggct tggcgccgcc gccgccgctc
     1981 accacgtaca tcacctcatc cctgaagtag cgctcgtaat tgtgcacgtg actgcccacc
     2041 acgagcacct ttgccagtag cgagccggca tgatgtgcca ggtagcgggc gatctccttc
     2101 tcatcgcgct cgcgtggaaa gaacggatcg ctaaccgggg gatagtgcag cacgatgagg
     2161 atgaacttca ccccagcgtc cgcgccggcc atctgcgcct cgaaccatga gcgctgctcg
     2221 ctccccggtt tcagcgcagc accggaatcg agcaccagcg cgagcaggtt ggagccgatg
     2281 gccaccgagt accagcgatg aggccgcagc ggcagatcat cgaacgcgct ccaccagttc
     2341 tcgaggcatg gctgcttatc cgcggcgcac cccctgaatt cgtgattgcc cagggccgga
     2401 tacactggga tgtgcgcctc cgaccaggcg cgggtttcgg tgcggaacgt ctggtagtcg
     2461 cccgggtcgc gcccctcgaa caccaggtcc ccaccgatca ggatcgctgc gggcttctcc
     2521 gtggcaattc tctccaccag cgcccgccgc gcggccgcat tgacgacatc cgggcgctgc
     2581 gtaaagcgcg tatcgccgta gaccacgagc acgagggggg cttcaccctg cgaagccaca
     2641 gtgaaacgcg gcggctccgc ggcgctggtg cgccgcgccg cgccgagcgc cgccaaggag
     2701 cacagcgcca gcatcgcgca cagtcccggg tccggcgcac gcatcggctt gctccgcgag
     2761 cgcgcgccgc ggcgattcag gcgccggaaa gcctacttgc tgccggggat cactttttgg
     2821 tcgatgcttc tgccaccttg atggccagtg cctcgacgta ggtggcctgg acgtgatcgc
     2881 ccttcttgat gttctgcagc tggctcgggt cttccgcctt gacatccacc tcgttgcccg
     2941 cagggccgcg cagggtgacc atctgcttgg cttgatcgac ggcgacgact tcagccgtca
     3001 cggtcacctt gtgcgcgatg gtgccgccgg gttttgcgcc gggtgccggc gcaggagtga
     3061 tcgctccctc cgcggtgggc gctggcaggg cgccgccgcc cttcttgagc tcgagcgcga
     3121 gagcttgggt gtacttggca tgaaccgtgt cgccgacctt gatctggtcg aagttgcgca
     3181 cttggtcacc cacctcgatg gtgcgcgtct tgccgctcga attctgcagc gtaacggtcc
     3241 gcatcgaggg gtcgacggcg actaccttcg cagtgacctc ggtcgtcgcc gctccggcca
     3301 cctttcccgg tgcgctggcg acccccgcct gagtggtcgg cgcagattgt gcctgcgcgc
     3361 cgagtgagac cgcccccatg ctcgccgccg cgagtgcagt cacgatcttg atgagtttca
     3421 ttcccggtct cccatgtggt gtgaaaagca gcgcaggccc cgcgccccgc taagatagac
     3481 ggtgtggggg acgggcgtgc cagtccccag ggcgggcatc ttaagcgaaa tgatgcgccg
     3541 aggcggccgc agtttgctac ccccgccgca cccagctgcc gcactgcaag gagagctcga
     3601 tgttgcagat agccgtgatc aacgagtcca ttgccatcgc cgatgcagat gtgcagagca
     3661 tgattgccgc tttcacgagc cagtggaaca aggacctgaa ctcggtgtgg ggcgtgggag
     3721 ccgctacgtt caccttcaac ccgaagcagc aggctccgcc cgcgggcgcc tggtgggtgg
     3781 tatttctcga caacagcgac caggccaacg cgctggccta tcacgatctc actaacgagg
     3841 ggctacccat ctcaaaagtg ttcgtcaaga cgatccaggc cgataacgcg agcctcagtg
     3901 tcggggcgag ccacgagatc tgcgagatgg cggtcgatcc gtggctgaac agcgcttacc
     3961 aggattttca gagtatcttt tgggcaggcg agatctgcga tccggtagag gacgaccaat
     4021 acggatacga catcggcggc gtacttgtaa gcgactttgt cacccccgac tggtttggac
     4081 ataagaacgc ggcgggcccc atcgatctga aacgccatgc cacgagcgct ttcgaagtgc
     4141 tgacgggcgg ctatgcacag aagttcgacc ccaagaaagg ctgggttcag gtgaccggcg
     4201 ccaaggcggc cctgacgctg cgcggatcgc gggcggcctc aggctcgcgc cgggagcgcc
     4261 gtacaagggg ctggaagaag tgggcgctca gccagcacac attcaggcgc tgacccggaa
     4321 accgttcccc cggcaattcc gcgcgaaatt ctgtcagagc gctcccgccg cgagcgccgg
     4381 tcatgctaag ttgacggcaa acgacggggg attgattggc ccagtccgaa gcaatggaat
     4441 ccaccgctgg gtggaccggg cggcgttcgc ccgatagccc acccgcaagc atcgcgccca
     4501 acggccaggg cgcgagcgct aggctggttg ccgacgtcga gggcattcgc gccctcaccg
     4561 ccgagtacga gcatctctat cgggtcaccg gcaacacgct gccctttgcg aggcaggagt
     4621 ggcatctcgc gtggtgcgag catctgctga gccaaacgcg gcgcgcctcg cagcagccgt
     4681 tgttctgcat cgtgcgcaac gccgatggca cctgcattgc gatcgtgccg ctcatcctga
     4741 cgctgcggcg actcggaccg ttgaaggttg cggcgctcgg actggtgggc gcagaccctg
     4801 cgctcaccga gatccgtaat ccgctcgttg cgcccggcta tgagcgcgcg acggtgtgcg
     4861 cggtgcacga gcagctggca aaggtgcgcg gctgggactg gattcagtgg agcgggatca
     4921 gtgcggcgct tgccgaagct ctgaaactgg aagccacacc gcagtggtac cagaccatcg
     4981 atgactacgt gctcgatctt cccgccgcct ggccagaaat gcgacagaaa ttcacacgca
     5041 acctgcgcga ggcgctgcgg cactgctaca actccctgcg ccgcgcgggg cacaacttcg
     5101 agttcgtagt ggcacgcgat cgcgtggagg tgcgctgggc gctctatcgc ttcctgcagc
     5161 tgcacacgtt gcgcgccaac atgccgtggg gcccgataca tgcggaccgt ttcaccgggc
     5221 gttcggtgcg ggcctttctc tacgatgtct gtgaccgcct ggcgcagcgc gatgtggtca
     5281 gggtctttca gctgcgcatc gccgatgaag tcgtcgcctc gcgtatcggt ttcgtggtgg
     5341 gcgacagcct ctacctgtac tactccggct tcgatcccgc ctgggggcgc ttca
//
LOCUS       DATDMP010000421         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2068062, whole genome shotgun sequence.
ACCESSION   DATDMP010000421 DATDMP010000000
VERSION     DATDMP010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068062"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VI195_07350"
     CDS             complement(<1..263)
                     /locus_tag="VI195_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6824241.1"
                     /translation="MMGFLLRAVISAIGLWLATQWVTGVHVDSVGTLILAGILLGVVN
                     AIVRPIAFILTLPITLLTLGLFLFVLNAAMVWLVSAILPGFHAP"
     gene            complement(260..709)
                     /locus_tag="VI195_07355"
     CDS             complement(260..709)
                     /locus_tag="VI195_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY6824242.1"
                     /translation="MGDRERGYAHRVDILAEASEVWRALTDPIQLARWCSPQAVIQAR
                     QGGLFRASVDRVTEFEAHIDVFEPERRLRLLYLPAASLPRADTVMVDDFILDRAPGGT
                     IVRLLGSGVPGAPQWDTQYWRLRTSWQQALTRLKVYVEMPARPGAPS"
     gene            753..1145
                     /locus_tag="VI195_07360"
     CDS             753..1145
                     /locus_tag="VI195_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY6824243.1"
                     /translation="MVYVQLVILLALLEFLLFGYAVGHARNRYKVSAPATGGHEIFER
                     YFRAQMNTLEQLIVFLPSIWLFARYLNAWAAAALGVLFIIGRALYFRGYVQAPESRHA
                     GFVLSAIPNVTLLVGALIGVMRSIWIAG"
     gene            complement(1186..1827)
                     /locus_tag="VI195_07365"
     CDS             complement(1186..1827)
                     /locus_tag="VI195_07365"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY6824244.1"
                     /translation="MLAWLIDLVLHLDRHLVELLARYDLWIYPILFAVIFAETGLVVT
                     PFLPGDSLLFAVGALAAVDTSGTLRAPVASLALGVAAVLGNTVNYGIGRAIGPPAFSG
                     RYRWLKVEYLRRTEEFFLRYGGMAVMVSRFVPIVRTCAPFVAGIGRMPFARFLAYNLV
                     GGFAWVLLFIWGGYLFGNIPFVKQHFGLVTLAIVTASLIPLYLALRRTRAPSG"
     gene            complement(1900..>2361)
                     /locus_tag="VI195_07370"
     CDS             complement(1900..>2361)
                     /locus_tag="VI195_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY6824245.1"
                     /translation="DGRTPEARFRMEASHGDLIEGAPLVVLVNGGSASASEIVAGALK
                     DHGRALLVGRRTYGKGSVQTVLPLSRGGAVKLTTSRYFTPSGASIHGKGIVPDVLEDG
                     PGDPPAELGAGKSADGPLTARDADVRLALAVLKERAGAPRLVSAVSRAFVP"
BASE COUNT          417 a          796 c          804 g          344 t
ORIGIN      
        1 ggtgcgtgga aacccggcag gatggctgac acgagccaca ccatggccgc attcagtacg
       61 aagagaaaca gcccgagtgt caggagggtg atcggcagcg tgaggatgaa ggcgatcggc
      121 cgcacgatgg cgttcaccac gccgagcaga attcccgcga ggatcagggt accgacacta
      181 tcgacgtgca cgccggtaac ccactgggtc gcgagccaca gtccgatggc gctgatcacc
      241 gcgcgcagca gaaatcccat catgaaggtg ctcccggtcg ggcgggcatt tcgacgtaga
      301 ccttgaggcg ggtcagcgcc tgctgccagc tcgtgcgcag ccgccagtac tgagtatccc
      361 attgcggggc acccggcaca cccgagccga gcagtcgcac gatggtgccg ccgggggcgc
      421 gatcgagaat gaaatcatcc accatcaccg tgtcggcccg cgggagcgag gctgccggca
      481 ggtacagcag ccgcaggcgc cgctcgggct cgaatacatc gatgtgcgcc tcgaactcgg
      541 tgacgcggtc cacgctcgcg cggaacaatc cgccctgacg ggcttggatc accgcctgcg
      601 gcgagcacca gcgggcgagt tgtatcggat cagtgagcgc ccgccacacc tcgctcgcct
      661 cggcgagtat gtcgacgcga tgcgcgtagc cccgctccct gtcacccatc tcggcattgt
      721 gccattctcg gtggcacaac gccggaggtc gcatggtata cgtgcaactg gtgatcctgc
      781 tcgcgctgct cgagttcctg ctgtttggct acgcggtcgg gcacgcccgc aaccgctaca
      841 aggtgtccgc ccctgccacc ggcggccacg agatattcga gcgttacttc cgcgcgcaga
      901 tgaacacgct cgagcagctc atcgtattcc tgccttccat ttggctgttt gcccgttacc
      961 tcaacgcctg ggccgcggcc gcgctcggcg tgctcttcat catcgggcgc gccctgtatt
     1021 ttcgcggcta cgtgcaggcg ccggagtcgc ggcacgcagg cttcgtgctc tcggccattc
     1081 cgaacgtcac gctgctcgtc ggtgcgctga tcggggtgat gcgctccatc tggatcgccg
     1141 gttgagaccc gcgcgccgcg cacacgctgt gggcgcgccg cggattcagc ccgagggcgc
     1201 gcgtgtccgg cgcaacgcca ggtagagagg aatgagcgag gccgtgacga tggcgagtgt
     1261 cacgagcccg aagtgctgct tgacgaaggg gatgttgccg aagagataac ccccccagat
     1321 gaacagcagc acccaggcga atccgcccac caggttataa gccaggaagc gggcaaaagg
     1381 catgcggccg attcccgcga cgaagggcgc gcaggtgcgc acgatcggca cgaagcgcga
     1441 caccatcacc gccatgccgc cgtagcgcag gaagaactcc tccgtgcggc gcaggtattc
     1501 gaccttcagc cagcggtagc ggcccgagaa ggcgggcggt ccgatggcgc ggccgatgcc
     1561 gtagttcacg gtattgccga gcaccgcggc cacgccgagc gcgaggctcg cgaccggtgc
     1621 gcgcagcgtg ccgctcgtat cgaccgccgc gagcgcgccc acggcaaaca gcagcgagtc
     1681 cccggggagg aagggcgtca ccacgagccc ggtctcggcg aagatcacgg caaagaggat
     1741 cgggtagatc cagaggtcat agcgtgcgag cagctcgacc agatgccgat cgagatgcag
     1801 cacgagatcg atgagccacg cgagcatggc gggggaggat atcggtaacg ctgccggcgt
     1861 gctaagcgtg ccgccggccc cccctgcgcc gttacggctt cacggcacga acgcccggct
     1921 cacggcggag acgaggcgcg gcgcgccggc ccgctccttg agcaccgcga gcgcgaggcg
     1981 cacatcggcg tcgcgcgcgg tcagcggccc gtcggcactc ttgccggcac cgagctccgc
     2041 cggcggatcg cccgggccgt cctcgaggac atcagggacg atccccttgc cgtggatcga
     2101 ggcacccgaa ggggtgaagt aacgcgaggt ggtgagcttc acggctcccc cgcgcgagag
     2161 cggcagcacc gtctgcacgg agcccttgcc gtaggtcctg cgcccgacga ggagcgcgcg
     2221 cccgtggtcc ttgagagcgc cggccacgat ctccgaagcg gaggccgagc ctccgttcac
     2281 gagcacgacg agcggtgctc cctcgatcag atccccgtgc gatgcctcca tgcggaagcg
     2341 ggcctcaggg gtgcggccgt c
//
LOCUS       DATDMP010000422         4322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1068179, whole genome shotgun sequence.
ACCESSION   DATDMP010000422 DATDMP010000000
VERSION     DATDMP010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068179"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1133
                     /gene="secF"
                     /locus_tag="VI195_07375"
     CDS             198..1133
                     /gene="secF"
                     /locus_tag="VI195_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY6824246.1"
                     /translation="MEFFSHQTSFPFMATRKVWYALSGILMVVSLGSFFGRGLNLGID
                     FTGGVSAEVSFPQAANVDAVRGALSAGGFREPQVQSFGSSRDIDIRLPPDPQQSPGEV
                     RARLETALHTVDAGAQVTQLDVVGPQVGNELKISAMWALFFTLLGIFLYIAFRFHTWR
                     LSLGAIFAVLHDPILVIGFFALTQMSFDLAVVAAILAVIGYSLNDTVVVFDRIRERFE
                     GNRRSPSNVILDQSINQTLSRTIMTKVVTSIVVVALLFLGGPVLKGFSAALLIGIIAG
                     TYSSIYISSAIALDFGLTAEHLFPSVKKAALDHLP"
     gene            1194..1850
                     /locus_tag="VI195_07380"
     CDS             1194..1850
                     /locus_tag="VI195_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY6824247.1"
                     /translation="MLEIVLGIDNIIFISILVERLPAAQRSAARISGLALAMLTRIAL
                     LFSVVGLTHLTRPWLEIAGRSFSGRDLVLLAGGLFLLGKSVLEIHQTLEGHAAGSERQ
                     RARGSFGAIVTQIAVIDIVFSLDSVFTAIGLVRELPVMVAAIVAAILVMMWVSRSIGA
                     FIARHPTIKMLALSFLLLIGVALVGEGLHFEIPKGYLYFAMAFAFGVELINMRLRPPR
                     "
     gene            complement(1884..3371)
                     /locus_tag="VI195_07385"
     CDS             complement(1884..3371)
                     /locus_tag="VI195_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6824248.1"
                     /translation="MASQLLATKSIAELHQQESSGNQLRRALNATHLTLLGIGGIIGT
                     GIFVLTGTAAANHAGPALPLSFIVAGLGCTFAGLCYAEFSAMIPVSGSAYSYSYATLG
                     EGVAWFIGWNLVLEYLFAVATVSVGWSGYAVSLLEQLHIHLPAALTNAPFGTPLGRES
                     FDIVRTGAIINVPAMLIVAVIGTICYIGIKQSAAFNSVIVAIKVTVIVLFILFGVSHI
                     NTANWHPFIPPNTGQFGVFGLSGIMGAAGVIFFAYIGFDAVSTAAQETRNPQRDMPIG
                     ILASLVICTILYVIVSAVLTGMVHYSELDVAAPVALALDKYPALHWLGIPVKLGAVCG
                     MTSVMLVMTIAQARIFFAMARDGLLPGWFGHVHPRFRTPSTGTVVTAVAAAVVGGLFP
                     VKILGELVSIGTLAAFVTVCVGVLILRRTRPDLPRPFRTPWPWFVCIAGALVCGAMMV
                     SLGPATWARLVVWTAVGVVIYAFYGYQHSALRKAAHGAAASARTA"
     gene            complement(3468..4268)
                     /locus_tag="VI195_07390"
     CDS             complement(3468..4268)
                     /locus_tag="VI195_07390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY6824249.1"
                     /translation="MQPLLNIAVRAARRAGEIIVRSMNHLDSIAITAKGRNDFVTEVD
                     RAAEQQIMASIRRHYPQHAFLAEESGRTGEHDTLWIVDPLDGTTNFLHGFPVFAVSIA
                     CQVRGRLEHAVIYDPLRQELFTASRGAGAHLDNHRLRVSKARTLEGALIATGFPYRAN
                     TRYLDAYLEMLRAVTLQAAGVRRPGAAALDLAYVAAGRVDAFWEIGLSAWDTAAGTLL
                     IQEAGGRIGTLSGAEYQQGGHVVAGTPKVYAALIELLAPHVPAELRAS"
BASE COUNT          717 a         1405 c         1442 g          758 t
ORIGIN      
        1 gccggcgtgg tgctgtgggt gctcggcacc ggcccgatcc gcggtttcgc catcgtgctg
       61 accctcggca tcctgacctc catgttcacc tcgctcatgg gcagccgggc gctgctcacc
      121 ctgatgtacg gcgggcagcg caaggtggcg cgtctgtcga tctgactgcc ggccgcgact
      181 gcgacaaagg acctcgagtg gaattcttca gccatcagac cagcttcccg ttcatggcca
      241 cacgcaaggt gtggtacgcg ctctcgggca tcttgatggt ggtatcgctc ggctcgttct
      301 tcgggcgcgg actgaatctc ggtatcgact tcaccggcgg cgtgagcgcc gaggtgagct
      361 ttccgcaggc cgccaacgtg gatgcggtgc gcggcgcgct ctccgccggc ggtttccgcg
      421 aaccccaggt gcagagcttc ggttcctcgc gcgatatcga catccgcctg cctccggacc
      481 cgcagcagag tcccggcgag gtgcgcgcgc gcctggaaac ggcgttgcat acggtcgacg
      541 ccggcgcgca ggttacgcag ctcgatgttg tcggtccgca ggtcggcaac gagctcaaga
      601 ttagcgccat gtgggcgctg ttcttcacgc tgctgggtat atttctgtat atcgccttcc
      661 gcttccacac ctggcggctg tcgctcggtg cgatcttcgc cgtgctgcac gacccgatcc
      721 tggtgatcgg cttcttcgcg ctcacccaga tgtcgttcga cctcgccgtg gtggcggcca
      781 tcctcgcggt catcggctac tcgctcaatg acacggtggt ggtgttcgac cgaattcgcg
      841 agcgcttcga gggcaaccgc cgttccccct cgaacgtgat actcgaccag tcgatcaacc
      901 agacgctctc gcgcaccatc atgaccaagg tggtgacctc gatcgtggtg gtggcgctct
      961 tgttcctcgg ggggccggtg ctcaagggct tctcggcggc gctgctcatc ggcatcatcg
     1021 ccggcaccta ctcctcgatc tacatctcca gcgccatcgc gctcgacttt ggactcaccg
     1081 ccgaacacct gtttccctcg gtgaagaagg cggcgctcga ccacctgcca taaccgagag
     1141 cccgtgctct gatcgccgac ccgcatctct ggctggcctt cctcgcgctg tcgctgctcg
     1201 agatcgttct cggcatcgac aacatcatct tcatctcgat cctggtggag cgcttacccg
     1261 cggcacagcg ctccgcggct cgcataagcg ggctcgcgct cgcgatgctg acgcgcattg
     1321 cactgctttt ctcggtggtg gggctgacgc acctcacgcg tccgtggctg gagatcgccg
     1381 ggcgcagctt ctccggccgt gacctggtac tgctcgccgg tggactgttc ctgctcggca
     1441 agagcgtgct cgagatccac cagacgctcg agggacacgc ggcggggagc gagcgtcagc
     1501 gagcacgcgg cagcttcggc gcgatcgtca cccagattgc ggtgatcgac atcgtgttct
     1561 cactcgactc ggtgttcacc gccatcgggc tggtacgcga gttgccggtc atggtggccg
     1621 ccatcgtggc agcgatcctc gtcatgatgt gggtgtcgcg ttccatcggc gccttcatcg
     1681 cccgccaccc gaccatcaag atgctggcgc tgtcgttcct gctgctgatc ggcgtcgcgc
     1741 tggtgggcga aggtctgcac ttcgagattc cgaagggcta cctgtatttc gccatggcgt
     1801 tcgcattcgg agtggagctg atcaacatgc ggctgcggcc gccacggtga gtcagtcgaa
     1861 cggcaggccg gcggtagcgc tgctcaggcg gtgcgggcac tcgccgcggc gccgtgcgcc
     1921 gccttgcgca gcgcgctgtg ctggtagccg tagaaggcat agatgaccac gccgaccgcg
     1981 gtccagacca ccagccgcgc ccaggttgcg ggcccgagcg ataccatcat ggcgccgcac
     2041 acgagcgccc cggcgatgca aacgaaccag ggccaggggg tgcggaacgg gcgtggcagg
     2101 tcggggcgtg tgcggcgcag gatcagtacc ccgacgcaca cggtgacgaa ggcggcgagc
     2161 gtaccgatcg acaccagctc cccgaggatc ttcaccggaa agagtccgcc caccaccgcc
     2221 gccgcgactg cggtgaccac cgtgccggtc gagggcgtgc ggaagcgcgg atgcacgtgg
     2281 ccgaaccagc ccggcagcag cccatcgcgt gccatggcga agaaaatgcg cgcctgagcg
     2341 atagtcatga ccagcatcac cgaggtcatg ccgcataccg cgccgagctt taccggaatc
     2401 ccgagccagt ggagcgcggg atacttgtcg agcgccaagg ccaccggcgc ggcgacatcc
     2461 agctcgctgt agtgcaccat gccggtgagc actgccgaga cgatgacata caggatggtg
     2521 cagatcacca ggctggcgag gatgccgatc ggcatgtcgc gctgcgggtt gcgggtctcc
     2581 tgtgcggcgg tcgacacggc atcgaagccg atgtaggcga agaagatcac gccggcggcg
     2641 cccatgatgc cgctcaggcc gaatacgccg aactgccccg tattcggcgg aatgaacggg
     2701 tgccagttgg cggtgttgat gtggctcacc ccgaacagaa tgaacagcac gatcaccgtc
     2761 accttgatgg cgacgatgac cgagttgaat gctgccgact gcttgatgcc gatgtaacag
     2821 atggtgccga tgaccgccac gatcagcatc gccggcacgt tgatgatcgc gccggtgcgt
     2881 acgatgtcga acgactccct gccgagcggt gtgccgaagg gtgcgttggt gagtgccgcg
     2941 ggcaggtgta tgtgcagctg ctcgagcagg ctcaccgcgt agccggacca cccgaccgag
     3001 acggtcgcca ccgcgaacag gtactcgagc accagattcc agccaatgaa ccaggccacg
     3061 ccctcgccga gcgtcgcata ggagtaggag taggcgctgc cggacaccgg gatcatcgcg
     3121 ctgaattccg cgtagcacag cccggcgaag gtgcagccga gtccggcaac gatgaacgac
     3181 agtggcagtg ccgggccggc gtgattggcg gccgcggtgc cggtgagcac gaagatgccg
     3241 gtaccgatga tgccgccgat accgagcagt gtcaggtgtg tggcgttcag cgcgcgccgc
     3301 agctggttgc cggaggattc ctgctggtgc agctcggcaa tcgacttggt tgcaagaagc
     3361 tggctcgcca cgacaccccc ggtgcgaatg ccagggactc tacacaagca ctctgtggaa
     3421 agcgagcaac cgaccgcagc cgcaacgtcg ctgcagaacg catgcgctca ggacgcgcgc
     3481 agctccgccg gaacgtgtgg cgcgagcagc tcgatgagcg ccgcgtagac cttcggcgtg
     3541 ccggcgacga cgtgtccgcc ctgctggtat tccgcgccgc tcagggtgcc gatgcgtccg
     3601 cccgcttcct ggatgagcag ggttccggcg gcggtatccc aggcactgag tcctatctcc
     3661 cagaacgcat cgacgcgtcc ggccgcgacg taggcgaggt cgagcgccgc ggccccgggg
     3721 cggcgcacgc cggccgcctg cagcgtgacc gcgcgcagca tctcgagata ggcatcgaga
     3781 tagcgcgtgt tggcgcggta gggaaagccg gtggcgatca gcgcaccctc cagcgtgcgc
     3841 gccttgctga cacgcaggcg gtgattgtcg agatgtgcgc cggcgccgcg gctggcagtg
     3901 aatagctcct ggcgcagcgg gtcgtagatc acggcgtgct cgagacgtcc gcgcacctga
     3961 caggcgatcg acacggcgaa caccggaaag ccgtgcagga agttggtggt gccatcgagc
     4021 ggatcgacga tccacaatgt gtcgtgctcg ccggtgcggc cgctctcctc ggcaagaaag
     4081 gcatgctgcg ggtagtggcg acggatgctc gccatgatct gctgctcggc ggcgcgatcc
     4141 acctcggtga cgaagtcgtt gcgacccttg gcggtaatgg cgatcgagtc gaggtggttc
     4201 atgctgcgca cgatgatctc gccggcacgg cgcgcggcac gtacggcaat gttgagcagc
     4261 ggctgcatga gcagcgttca tcatagcaac gctcactgcg ctctgccgac cgccgtcaat
     4321 ct
//
LOCUS       DATDMP010000423         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1511722, whole genome shotgun sequence.
ACCESSION   DATDMP010000423 DATDMP010000000
VERSION     DATDMP010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511722"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VI195_07395"
     CDS             complement(<1..669)
                     /locus_tag="VI195_07395"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY6824250.1"
                     /translation="MRALPLSPSVLSDARSVAAYGIFFASYFVFALGKFPGLKIDRPG
                     AAIIGAVLMVAFRIMSAQQALSAIDFSTIVLLFSMTLIVAHLHLGGFFEWIAELTVLR
                     LNERQLLAVVVFTSGILSAFLVNDIVCLVMTPFIIGVARRLGVEPIPYLLALATASNI
                     GSVATITGNPQNMLIGSVSGIGYLDFLAHLGPVAVGGLILDWALLHWLYLRRKPAAQV
                     PAVAR"
     gene            complement(671..1282)
                     /locus_tag="VI195_07400"
     CDS             complement(671..1282)
                     /locus_tag="VI195_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY6824251.1"
                     /translation="MPAPPTIRNIVFDIGWVLVRLNYRPLLDFLRRHGAQLADRDSVM
                     RHIPLEDHETGRLPGHGLLERLHGLTRSERASREELRAKWLDMFELEGRMIELAHRLS
                     ERYRVFLLSNIGDLHWAHLAREYRLHAIGQGALPSYVAGVMKPHPRIYAEAERRFALE
                     PPSTVFIDDRADNVLTARSRGWQGIVHQGYPDTLARLQGLGVG"
     gene            complement(1313..>2009)
                     /locus_tag="VI195_07405"
     CDS             complement(1313..>2009)
                     /locus_tag="VI195_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824252.1"
                     /translation="LAAGLGVRVRGLSFHVGSQVPDAARHVEAVVACVKLMAGARRER
                     LGSFDTLDIGGGFPIDYVQPVQDIGRFCAPLRAALARLPKRVRVIAEPGRYIVGPAAI
                     GVATVIGRARREGHWWYYLDDGLYGAYSGQLFDQGRYPIEPLRANGERLPSVLAGPTC
                     DSIDVIAENLMLPPLKVGDLIAGRAMGAYTWACATEFNFFPKPAVVAVNRHPGDAGGV
                     ARLASAAPSHTAL"
BASE COUNT          364 a          674 c          692 g          279 t
ORIGIN      
        1 gcgcgcaacc gccggcacct gcgccgcagg tttgcgccgc aggtatagcc aatgcagcaa
       61 agcccagtcg agaatcagcc cgccgacggc caccgggccg aggtgcgcga ggaaatcgag
      121 gtagccgatc ccggacaccg agccgatgag catgttctgc ggattgccgg ttatcgtggc
      181 cacgctgccg atgttcgagg ccgtggcgag cgcgagcaga tagggaatcg gctcgacgcc
      241 caggcgtcgg gcgacaccga tgatgaatgg cgtcatgacc agacagacga tgtcgttcac
      301 caggaaggcg gacaggatgc cgctggtgaa gaccaccacc gccagcagct gccgctcgtt
      361 cagccgcagc accgtcagct ccgcgatcca ttcgaagaag ccgccgagat gcaggtgggc
      421 aacgatcagc gtcatggaga acagcagcac gatcgtggag aaatcgatcg cgctcagcgc
      481 ctgctgcgcg ctcatgatgc gaaatgccac catcagcacc gcgccgatga tggccgcgcc
      541 cgggcggtcg atcttcaggc cgggaaactt gcccagcgca aagacgaagt agctcgcaaa
      601 gaagatgccg taagcggcga cgctgcgtgc atcggagaga acgctcgggc tcagcggcag
      661 cgcgcgcacg tcagccgacc ccgagaccct gcaggcgcgc gagcgtgtcg gggtagcctt
      721 ggtgcacgat gccctgccag ccgcggctgc gggcagtcag cacattgtcg gcgcggtcat
      781 cgatgaacac ggtggacggg ggctcgagcg cgaagcgtcg ctcggcttcg gcgtagatgc
      841 gcggatgcgg cttcatgacg ccggccacgt aagagggcag cgctccctgg ccgatggcgt
      901 gcaggcgata ctcgcgcgcc aggtgcgccc agtgcagatc gccgatgttc gacagcagga
      961 acacgcggta gcgctcgctc aggcgatgcg cgagctcgat catgcggccc tcgagctcga
     1021 acatgtcgag ccacttcgcc cgcaactcct cgcgcgaggc ccgctcgctg cgggtgaggc
     1081 cgtgcagccg ctcgagcagc ccgtggcccg gcagccgtcc ggtctcgtga tcctcgagcg
     1141 ggatgtgccg catcacactg tcgcggtccg cgagctgtgc accgtgtctc cgcagaaaat
     1201 cgagcagcgg gcgatagttg agccgcacca acacccaacc gatgtcgaac acgatgttgc
     1261 ggatggtcgg cggcgccggc atgagctgaa cccgaggcga gtgcggcggg gattataggg
     1321 cggtgtggct cggcgctgcg gacgcgagcc gcgccacgcc cccggcatcg ccggggtgcc
     1381 ggttcaccgc caccacggcc ggcttgggaa agaagttgaa ttccgttgcg cacgcccagg
     1441 tgtatgcgcc catggcacgc cccgcgatga gatcgcccac cttcaacggc ggcagcatga
     1501 ggttttcggc gatcacatcg atgctgtcgc aggttgggcc ggcgagcacc gaaggcaacc
     1561 gctcaccgtt cgcgcgcagc ggctcgatcg ggtaacgtcc ctgatcgaac agctgcccgc
     1621 tgtatgcgcc gtacagtcca tcatcgaggt agtaccacca gtgcccctcg cggcgcgcgc
     1681 gtccaatcac ggtagcgacg ccgattgccg cgggcccgac gatgtagcgg cccggctcgg
     1741 cgatcacgcg tacccgcttc ggcagccgcg cgagcgctgc gcgcagcggc gcgcagaagc
     1801 ggccgatatc ctgcaccggc tgcacataat cgatggggaa gcccccgccg atgtcgagcg
     1861 tgtcgaagct gccgaggcgc tcgcgccgcg cgccggccat gagcttcacg cacgccacga
     1921 ccgcctcgac gtgcctggcg gcatcgggca cctgcgagcc gacgtggaag gagaggccgc
     1981 gcacgcgcac cccgagtccg gcggcgagc
//
LOCUS       DATDMP010000424         3651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1290816, whole genome shotgun sequence.
ACCESSION   DATDMP010000424 DATDMP010000000
VERSION     DATDMP010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290816"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VI195_07410"
     CDS             <1..260
                     /locus_tag="VI195_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6824253.1"
                     /translation="QREFTAATGLAPHRWLVAERIERAKELLETTRLSAEDIAARVGL
                     GSAESLRHHFRRRLGTTPQQYRRRFSRRAEAAGNRGRARTQ"
     gene            complement(254..1579)
                     /locus_tag="VI195_07415"
     CDS             complement(254..1579)
                     /locus_tag="VI195_07415"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEY6824254.1"
                     /translation="MLTSLLLLAGCGGSGTTTNSGGGGGGGGGGGGGGSQVIANPGPP
                     NVESLTVDGGPTQLTTTAVNTAYITVTVCMPDGKFDNCQNIDHIEVDTGSIGLRLIAG
                     PSTGPSAGELTLALPAVADPMNPSNVLAECLEFADGFSWGSVNVADVALPVSGESATN
                     VTVHVIGAASAGDPGNANPTCVPPPPLVTENTVPSFGANGILGVGPFVNDCNSTGDCD
                     PLAQVCSGGHCYPGNSATYFTCTAAASCTQVAKGTVNAQQQLPNPAVLFATDNNGVIV
                     ELPTVGDSGAAGPVNGSLVFGIGTQANNSLGSATQLLADPLSGVISASLSGTTYPDSY
                     LDSGSNANFFPTAIASCPQPNQGFLCPPATTSESATLQGTNGNVLTADFSVANANTLF
                     MNNYAAFSNLAGNNTASASSVDLGLSFFYGRNIFTGFENPNVSAPYFAY"
     gene            complement(1624..2076)
                     /locus_tag="VI195_07420"
     CDS             complement(1624..2076)
                     /locus_tag="VI195_07420"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEY6824255.1"
                     /translation="MMKLPCTLLGMLAAVYVSAPALAALGGDAASVETDRASMKGVTR
                     VTSAVEYSVHEIQSPAGTVVHEYVSAQGKVFAVSWRGPGLPDLAQLLGGYSQRLQQAA
                     SHPHYNHHRLNIAAPEVLMQSGGHLRSRYGRAWVPGLLPQNFSLKDLG"
     gene            2366..2442
                     /locus_tag="VI195_07425"
     tRNA            2366..2442
                     /locus_tag="VI195_07425"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2400..2402,aa:Asp,seq:gtc)
     gene            2470..>3651
                     /locus_tag="VI195_07430"
     CDS             2470..>3651
                     /locus_tag="VI195_07430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6824256.1"
                     /translation="MLQRIRDSLQGQKWLAYLLLGALALVFAAWGAYGIVNLNFGGAN
                     YAAEANGSKISLEDARNVWLRQQSMWQQRLGGAELPQPLRARLQDQVLESLIRRALIT
                     ERSHDLGYRVSNQALLEAVQAEPAFQIDGKYDPQAAKAALAQAGVSLDTYTQQLRTDL
                     QQLQLEGGIRASDFVTPSELTRLTDLEDQQREVRYLVLPADRFRASAAIDDAAVQAYY
                     KEHLAQYMRPESVDLQYGELRLEALAAQQEVSDADLRAAYEKEKSRLEVPEKRHARHI
                     LISGKDDAAALALAQQVLSQAKSGKDFGELAKQYSQDPGSAQNGGDLGWAERSTFVKP
                     FADALFGMSVGEITGPVKTQYGYHIIRLEEIQRGKSKSFEEARPELEAQQRRARATDR
                     VG"
BASE COUNT          634 a         1158 c         1239 g          620 t
ORIGIN      
        1 tgcagcgtga attcaccgca gccaccgggc tcgctcccca tcgctggctg gtggcggaac
       61 gcatcgagcg cgccaaggag ttgctggaaa cgacgcgcct ctccgccgag gacatcgccg
      121 cgcgcgtcgg tctcggcagt gccgagtcgc tgcgccatca tttccgccgc aggctcggaa
      181 cgacgccgca gcagtaccgg cggcgtttct cgcgccgcgc ggaggccgcc gggaaccgcg
      241 gccgcgcccg gactcagtag gcgaagtacg gtgccgagac gttggggttc tcgaaccccg
      301 tgaagatgtt gcggccgtag aagaacgaca gcccgagatc gaccgagctc gcactggcgg
      361 tattgttgcc ggcgaggttg gagaacgcgg cgtagttgtt catgaacagc gtattcgcgt
      421 tcgcgacact gaaatccgcc gtcaatacgt taccgttggt gccctgcagc gtcgcgctct
      481 cgctcgtggt cgcgggaggg cacagaaagc cctggttggg ttgtgggcag ctcgcaatcg
      541 cagtcgggaa gaaattggca ttcgagccgc tgtcgaggta gctgtcggga tacgtcgtgc
      601 cactcagcga agcgctgatg acgccgctca agggatcggc caggagctgg gtggcgctgc
      661 cgagcgagtt attcgcttgg gtcccgatgc caaataccag cgacccgttg acaggtcccg
      721 cggcaccgct gtcgcccact gtcggcagct cgacgatcac cccgttgttg tcagtggcga
      781 acaacaccgc cgggttcggc agttgctgct gcgcattcac ggttcctttg gcgacctggg
      841 tgcagctggc tgctgccgtg caggtgaagt aggtcgccga gttccccggg taacagtggc
      901 cgccactgca gacctgtgcc agcgggtcgc agtcgcccgt gctgttgcag tcattgacga
      961 agggtccgac gccgaggatg ccgttcgcgc caaaggaggg aacggtgttc tccgtgacga
     1021 ggggcggggg cgggacgcag gtcggattgg cgttgccggg gtcaccggcc gaggccgctc
     1081 cgatcacgtg cacggtgacg ttggtcgcac tctcgccgga caccggcagc gcgacatcgg
     1141 ccacgttcac cgagccccag ctgaagccgt cggcaaactc aaggcactcg gcgagcacgt
     1201 tgctcggatt catcggatcc gccaccgccg gcagggccag ggtgagctct ccggcgctcg
     1261 ggccggtgga gggacccgcg atcagccgca gacctatgga accggtatcc acctcgatgt
     1321 gatcgatgtt ctgacagttg tcgaacttgc catcgggcat gcacacggtc acggtgatgt
     1381 aggcggtgtt caccgccgtg gtggtcaact gtgtcgggcc gccatcgacc gtgaggcttt
     1441 cgacgttggg cgggccaggg ttcgcgatga cctggctgcc acccccgcca ccgccgccgc
     1501 caccaccacc tccgccgcca ctgttggtcg tcgtgccgct gccgccgcag ccagccagca
     1561 gcaggaggga agtcagcatc agagtcagga gcttacgcac aggcaatcct cttccttgtg
     1621 ccatcagcca aggtccttca gcgagaaatt ctgtggaagc agccctggca cccaggcgcg
     1681 tccgtagcgg ctgcgcagat gtccccccga ctgcatgagg acctccggag ctgcgatgtt
     1741 cagccggtga tggttgtagt gaggatgact ggcggcctgc tgcaggcgtt gcgagtagcc
     1801 gcccagcagc tgcgcgagat cggggagccc gggtccgcgc cagctcacgg cgaagacctt
     1861 gccctgcgcc gacacgtact cgtggaccac cgttccggcg ggactctgga tctcatggac
     1921 cgagtactcg actgcggagg tgacgcgcgt gacacccttc atgctcgcgc ggtccgtctc
     1981 gaccgaggcc gcatcgccgc cgagcgccgc gagcgccggc gcggagacat ataccgcggc
     2041 gagcataccg agcagagtgc agggcaactt catcattggc cggatcctca caagccttcg
     2101 cggcattgtg cctgacggaa cgctgtcgtg cgcagcgtgt cttaacttgg cgacggactg
     2161 gcacgcaatt aagttatcgg gtgttagcgc ctggcagtac gaagcgcttc aatggaggat
     2221 cggttgaccg ccgaggtcgg cggactctgc tgcctttggc caggacgccg ccattgccgg
     2281 gcgccacggg cccggctgcg gcggggccaa gcgaaatcgc ttgtagcctt attgttcaat
     2341 ccgataggat tcgccccccc aaagcggagc ggtagttcag ctggttagaa taccggcctg
     2401 tcacgccggg ggtcgcgggt tcgagtcccg tccgctccgc cacgttctcc gacatcagcc
     2461 aaccgacaca tgcttcagag gatccgagac agtctgcagg gccagaaatg gctcgcatat
     2521 ctgttgctgg gcgcgctcgc tctcgtgttt gccgcctggg gggcttacgg catcgtcaac
     2581 ctgaatttcg gcggcgcgaa ctatgccgcc gaggcgaacg gcagcaagat ctcgctcgag
     2641 gatgcacgta atgtctggct gcgtcagcag agcatgtggc agcagcgcct cggtggcgcg
     2701 gagctgccgc agccgctgcg cgcgcggctg caggaccagg tgttggaaag cctgattcgg
     2761 cgcgcgctca ttaccgagcg ctcccacgat ctgggctacc gcgtgagcaa ccaggcgctg
     2821 ctggaagcgg tgcaggccga gcccgcattc cagatcgatg gcaagtacga cccgcaggcg
     2881 gcgaaggccg cactcgcgca ggcgggcgtc tcgctcgaca cctacacgca gcagctgcgc
     2941 accgatctgc agcagctgca gctcgaaggt ggcatccgcg cctcggactt tgtcacaccg
     3001 tccgagctca cgcggctgac cgacctcgaa gaccagcagc gcgaggtgcg ctacctggtg
     3061 ttgcccgcgg accgttttcg cgcctcggcg gccatcgacg atgccgccgt acaggcgtac
     3121 tacaaagagc acctggcgca gtacatgcgg cccgagtcgg tcgatctgca gtacggtgag
     3181 ctgcgcctcg aggcgctcgc tgcgcagcag gaagtgagcg atgcagacct gcgtgccgcg
     3241 tacgagaagg agaagagccg gctggaagtt cccgagaagc gccacgcacg gcacatcctg
     3301 atcagcggta aggacgatgc cgcggccctc gcacttgcgc agcaggtgtt gtcacaggcg
     3361 aagtccggca aggattttgg cgagctggcg aagcagtatt cgcaggatcc gggctcggcg
     3421 cagaacggcg gtgatctcgg ctgggcagag cgcagcacat tcgtgaagcc ctttgcggat
     3481 gcgctgttcg gcatgtccgt gggtgagatc accggacccg tgaagacgca gtacgggtat
     3541 cacatcatcc gcctcgaaga gatccagcgc ggcaagtcga agagtttcga ggaggcgcgc
     3601 cccgagctcg aggcgcagca acggcgcgcg cgcgcgacgg atcgcgtcgg t
//
LOCUS       DATDMP010000425         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1290953, whole genome shotgun sequence.
ACCESSION   DATDMP010000425 DATDMP010000000
VERSION     DATDMP010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290953"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VI195_07435"
     CDS             complement(<1..314)
                     /locus_tag="VI195_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824257.1"
                     /translation="MGRSDRSYRSSLGKFAGITAGAALASACWLPQAHADEAAVLEEV
                     TVTAQRRTERLQDVPVAVTALSESELIDRGVRQAGDIVDAVPNLLLNLPYGPEAQPTF
                     TLR"
     gene            551..1627
                     /gene="obgE"
                     /locus_tag="VI195_07440"
     CDS             551..1627
                     /gene="obgE"
                     /locus_tag="VI195_07440"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY6824258.1"
                     /translation="MKFVDEARARVQAGNGGRGSTSFRREKFVPFGGPDGGDGGHGGS
                     VYLRAASGINTLADFRIERTFKAAHGEPGRSNDCTGRSGADLYIPVPIGTVVRDAGTL
                     EVLGDLTRDGEQLLVARGGKGGWGNQRFKSSTNRSPRQFGPGLPGEKRALEFEMQVIA
                     DVGLLGLPNAGKSTLIRAVSAARPKVADYPFTTLHPNLGVVAVSEHRSFVMADIPGLI
                     EGAAEGAGLGVRFLRHLQRTRLLLHLIDIAPPDPQVDPVREARAIVAELRKFSRELAA
                     RPRWLVLNKRDLLPGADAEQRARSIVRRLRYRGPSFLISGATGEGTRSLCEAAMSFLE
                     EHDRAARGRASCPADAAGAQPDAT"
     gene            complement(1712..1975)
                     /gene="rpsT"
                     /locus_tag="VI195_07445"
     CDS             complement(1712..1975)
                     /gene="rpsT"
                     /locus_tag="VI195_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY6824259.1"
                     /translation="MANTKSAEKAAHQAEKHRARNVALRSRMRTAVRAVTAAIAGGDK
                     EAARVSYQKAVPEIDSLVGKQIIHRNKAARHKSRLAARIRAMQ"
BASE COUNT          310 a          716 c          735 g          358 t
ORIGIN      
        1 cgcagcgtga aggtcggctg cgcctccggt ccgtagggga gattcagcag caggttgggg
       61 accgcatcga cgatgtcgcc cgcctggcgt acgccgcgat cgatgagctc gctctcggag
      121 agcgcggtga ccgcgaccgg cacatcctgc agccgctcgg tgcggcgctg ggcggtgacg
      181 gtcacctcct cgagcacggc ggcttcatct gcatgcgcct gcggcagcca gcaggcgctc
      241 gcgagcgcgg cgcccgcggt gatgcctgcg aacttaccga gggatgaccg atagctgcga
      301 tcactgcggc ccatggcagc gcctccccta acagcgtgcc tgctggccgc ccggcggcat
      361 ccctgccacg cggcagccgt ggcacatcga ttatcgatag tttcttctcg ccaacgtaac
      421 gaactgtagg acgagtgcct gcgagcgtca accgcatgac cgcagcgccc gagggtgttt
      481 ccggtcggcg cgaggccgcc ccccgcctga ctgggcgccg cgtcggccgc gatgcgctag
      541 agtcatccgc atgaagttcg tcgatgaggc acgggcgcgc gtacaagcgg gcaacggcgg
      601 ccgcggcagc accagtttcc gccgtgagaa gttcgtgccc ttcggtgggc ccgatggtgg
      661 cgatggcggc cacggcggca gcgtctacct gcgcgctgct tccgggatca atacgctcgc
      721 ggatttccgc atcgagcgca ccttcaaggc cgcgcacggc gagcccggcc gcagcaacga
      781 ctgcaccggg cgcagcggcg ccgatctcta cattccggtg ccgatcggca ctgtggtgcg
      841 cgatgccggg acgctcgagg tgctcgggga tctcacgcgc gatggcgagc aactgctggt
      901 ggcgcgtggc ggcaagggcg ggtggggaaa ccagcgcttc aagtcgagca ccaaccgctc
      961 accgcgccag ttcggcccgg gattgcccgg cgagaagcgc gcgctggaat tcgagatgca
     1021 ggtgatcgcg gatgtgggcc tgctcggcct gccgaatgcc ggcaagtcga cgctcattcg
     1081 tgcggtgtcc gcggcgcggc ccaaggtcgc cgactacccg ttcacgaccc tgcacccgaa
     1141 tctcggggtg gtcgcggtaa gcgaacaccg cagtttcgtg atggccgaca tccccgggct
     1201 catcgagggc gcggcggagg gtgcgggcct cggtgtacgc ttcctgcggc atttgcagcg
     1261 cacgcggctg ctgctgcacc tgatcgacat cgcgccgccc gatccgcagg tcgatccggt
     1321 gcgcgaggca cgtgcgatcg tcgccgagct gcgcaagttc agccgcgagc tcgcggccag
     1381 gccccgctgg ctggtgctca acaagcgcga tctgctcccc ggcgccgatg ccgaacagcg
     1441 cgcgcgcagc atcgtgcgac ggctgcgcta tcgcgggccg tctttcctca tctcaggggc
     1501 gacgggcgaa gggactcgtt cgctatgcga ggccgctatg agttttttgg aggagcacga
     1561 tcgagcggca cgcgggcgcg catcatgtcc agcagatgct gccggcgcgc agccggacgc
     1621 aacttgagcg gtgtcggaat gacggggacg ggaatgggtg ccttttcctt cattgccaga
     1681 tccttcgctg caacatcctt cattgcaagc ctcactgcat ggcgcggatg cgagccgcaa
     1741 gccggctctt gtgccgcgca gccttgttac ggtggatgat ctgcttgccc accagtgagt
     1801 cgatctcggg gaccgccttc tggtagctga ccctggccgc ctccttgtcg ccgcccgcga
     1861 tcgctgcggt caccgcgcgc acggccgtgc gcatgcgcga gcgcagcgcg acgttgcggg
     1921 cgcgatgctt ctcggcctgg tgggcagctt tctcggccga cttggtattg gccacgaaat
     1981 ctccggtttc ccgctacgtt ccgggtgcgg ggggccggta gtctgcgcat cggtccgggg
     2041 cttgtcaacg cgcttggccg ccccggcgcg cacccaccgg cgcattcaca taaactggcc
     2101 agcctcacgg tcacgccat
//
LOCUS       DATDMP010000426         3635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1512014, whole genome shotgun sequence.
ACCESSION   DATDMP010000426 DATDMP010000000
VERSION     DATDMP010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512014"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VI195_07450"
     CDS             complement(<1..383)
                     /locus_tag="VI195_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY6824260.1"
                     /translation="MPREPEHFRQTARPVTVAAIQMACDWDSDENIARAERLVRSAAA
                     RGAQIILLPELFETPYFCIEQDVRHLALAHELAHDPAVQRFARVARELGVVLPISFFE
                     RAGQARFNSIAILDADGRTLGVYRKS"
     gene            complement(471..1433)
                     /locus_tag="VI195_07455"
     CDS             complement(471..1433)
                     /locus_tag="VI195_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6824261.1"
                     /translation="MQKIRTAVVGVGYLGRFHAQKYAQAAGCELVGVVDSRGEAREPV
                     AAELGTRPLAEHRSLLGAVDAVSIVTPTPAHFSIAREFLEAGAHVLVEKPITETPEEA
                     RELIAIAARAQRILQVGHLERFNSAVLAAEPYLRSPRFVECHRLAPYRERGTDVNVVL
                     DLMIHDIDIVQTIVGAPIETIDAIGTPVFSEGIDIANARIRFANGCIANATASRVSVK
                     TERKLRIFEDDAYLSLDLQQKILTVIRKRTPVDGPGPLPVTIEEQSLEPGDALKAEID
                     SFLECIRSGRAPVVPGEAGLVALETAMRITDQVERSLAQRNSQA"
     gene            1458..1865
                     /locus_tag="VI195_07460"
     CDS             1458..1865
                     /locus_tag="VI195_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824262.1"
                     /translation="MSSPPPIKAPARPFERPMTPPQDDGETPRITPRMRRELIVYGGA
                     LLFGLIGVPLVIWLGGNRVLGPYTHGQNLHAGPLALLQDYYLGLLHGSAVFWWVALGP
                     AALLLLLRLFIRLVRRMPARREEPEPTATRRAR"
     gene            complement(1876..2130)
                     /locus_tag="VI195_07465"
     CDS             complement(1876..2130)
                     /locus_tag="VI195_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824263.1"
                     /translation="MGSTVALAAGLLLTWIVILFLPEEQARFAPEHAALVRAVLVFTV
                     FAAVTAGSFYGEQRERRWRLAAHAATLAMFAVTVWLYWPK"
     gene            2248..>3635
                     /locus_tag="VI195_07470"
     CDS             2248..>3635
                     /locus_tag="VI195_07470"
                     /inference="COORDINATES: protein motif:HMM:NF037004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY6824264.1"
                     /translation="MPLLLRRSSGWITAAALAVMALALAGSVQVPASTGLLPPGSIIP
                     TDSQRSTARKIGRILEDQHYSRAAIDDKLSDVVYERYLEFLDGQRSYFLASDISDFSI
                     YRYRFGDMIRTGNIDPAYLIFARFQQRNRERMQQALTLLQSEPDWTLNETFEFDRSHS
                     PWPADGQAMQELWRKRVKNDALSLMLTGKTWPEAADILRKRYERVLRRVDQVTSEDVF
                     ENLMNAYARAFDPHSSYFSPRSSEEYRIQMSLNYEGIGASLQLVDDYVTIMNVLEGGP
                     AAVAGTLKTNDRIVGVGQGHDGPFTDVIGWRLDDVVQLIRGKANTMVRLQVLPAGAVP
                     GSGEKVLEFVRNKVTLEAQAAHKEVKSVVRNGRTLKIGVITVPGFYQDIAAQNAGDQN
                     YRSTTRDVLKLINQLKAEKIDGLVLDLRNDGGGYLPEATALTGLFIDHGPVVQLRDTS
                     GRLEVLDDPQTGP"
BASE COUNT          632 a         1217 c         1194 g          592 t
ORIGIN      
        1 gacttgcggt acaccccgag ggtgcggcca tccgcgtcga ggatggcgat ggagttgaag
       61 cgcgcctggc cggcacgctc gaagaagctg atcggcaaca cgacgccgag ctcacgcgcc
      121 acacgcgcga agcgctgcac cgccgggtcg tgggcgagct catgcgcgag cgcgagatgg
      181 cgcacgtcct gctcgatgca gaaatagggg gtctcgaaga gctccgggag caggatgatc
      241 tgcgcgccgc gggctgccgc gctgcgcacc aggcgctccg cccgggcgat attctcgtcg
      301 ctgtcccaat cgcaggccat ctggatggcc gcgacggtca ccggccgcgc agtctgccgg
      361 aaatgctcgg gctcgcgggg catggaagtc ctgcttctcg cctgcgggcg atccccgcgc
      421 ggtgattcta cgcgcgaaag ccgcgacagc ggctcgcgct cgcgtggcgg tcaggcctgg
      481 ctgttgcgct gcgccagtga gcgctcgacc tgatcggtga tacgcattgc ggtctcgagc
      541 gccaccagcc ccgcttcccc cggcacgacc ggcgcgcgcc cgctgcggat acattcgaga
      601 aaggaatcga tctccgcctt caacgcatcg ccgggctcga gactctgttc ctcgatggtt
      661 accggcagcg gcccgggccc gtcgacgggt gtgcgcttgc ggatgaccgt gaggatcttc
      721 tgctgcaggt cgagtgacag gtaagcgtcg tcctcgaaga tccgcagctt gcgttcggtc
      781 ttgacgctga ctcgcgaggc cgtggcgttg gcgatgcagc cgttcgcaaa acgaatgcgc
      841 gcgttggcga tgtcgatgcc ctcggagaac accggggtgc cgatggcgtc gatggtctcg
      901 atcggcgcac cgacgatcgt ctgcacgata tcgatatcgt ggatcatcag atcgagcacc
      961 acgttcacat ccgtgccgcg ctcgcgataa ggggcgagcc ggtggcactc gacgaagcgc
     1021 ggcgagcgca ggtagggctc ggccgcgagc actgcggaat tgaaacgctc caggtggccc
     1081 acctgcagga tgcgctgcgc gcgggcggcg atcgcgatca gctcgcgcgc ctcctcggga
     1141 gtctcggtga tcggtttctc caccaggacg tgcgcgccgg cttcgagaaa ctcgcgcgcg
     1201 atggaaaagt gcgccggcgt cggcgtgacg atgctcaccg catccaccgc cccgagcaga
     1261 ctacgatgct cggcgagggg gcgggtgccg agctctgcgg cgaccggctc gcgcgcctcg
     1321 ccacgggagt cgacgacgcc gaccagctcg caacctgcgg cctgggcata tttctgcgca
     1381 tgaaagcgcc cgagatagcc caccccgacc accgcggttc ttattttctg catcgcaacg
     1441 gtattatgcc cgcacccatg tcgagtccgc ccccgatcaa ggcccctgcc cgtcccttcg
     1501 agcgcccgat gaccccgccc caggacgatg gggaaacgcc gcggattacg ccccggatgc
     1561 ggcgcgagct gatcgtctat ggaggtgctc tgcttttcgg tctcatcggg gtgccgctag
     1621 tgatctggct gggcggtaat cgcgtgctcg gaccctacac ccatgggcag aacctgcacg
     1681 cgggcccgct cgcgctgctg caggactact acctcggact gctgcatggc tcggcagtat
     1741 tctggtgggt ggcgctcgga cccgcagcgc tgctgctgct gctgaggctc ttcatccggc
     1801 tggttcgtag gatgccggcc cggcgggagg agcccgagcc gacggcgacc cgcagggccc
     1861 gctagcgcac ttgtcttatt tcggccaata gagccatacc gtcacggcga acatcgcgag
     1921 cgtggccgcg tgtgcggcca gccgccagcg gcgctcccgc tgttccccat agaagcttcc
     1981 ggcggttacg gccgcgaaaa cggtgaacac gagcaccgcg cgcaccagcg cggcgtgctc
     2041 gggggcgaag cgtgcctgct cttccggcag gaacaggatc acaatccagg tgagcaggag
     2101 ccctgcggcc agggcgaccg tcgatcccat gagaatggcc agaagcgccg ccaggggccg
     2161 catggatgct gtgagtgccg tacttgctga gacgaaccgc tcgctctagc cttactctaa
     2221 gaatctaccg aagctaagag ccttacaatg cctttactcc tgcgccgatc ctcgggatgg
     2281 ataactgccg ccgctctggc ggtcatggcg ctcgcactcg ccgggtcagt gcaggttccg
     2341 gccagtaccg gactgctgcc cccagggtcg atcatcccga ccgacagcca gcgctccacc
     2401 gcccgcaaaa tcggccgcat tctcgaggac cagcactaca gccgtgcggc catcgacgac
     2461 aagctgtcgg atgtggtgta cgagcgctac ctcgaattcc tcgatggcca gcgcagctac
     2521 ttcctcgcga gcgacatcag cgacttcagc atctatcgct atcgcttcgg cgacatgatc
     2581 cgcaccggca acatcgatcc ggcctacctg atcttcgcgc gcttccaaca gcgcaatcgc
     2641 gagcgtatgc agcaggcgct cacgctgctg cagagcgagc ccgactggac actgaacgag
     2701 accttcgagt tcgaccgctc gcactctccc tggcccgccg acgggcaggc gatgcaggag
     2761 ctgtggagga agcgcgtcaa gaacgatgcg ctgtcgctga tgctcaccgg caagacctgg
     2821 ccggaggccg ccgacatcct gcgcaagcgc tacgagcgcg tgctgcgccg cgtggaccag
     2881 gtgacgagcg aggacgtgtt cgagaatctg atgaacgcct acgcgcgcgc cttcgatccg
     2941 cactcgagct acttttctcc gcgcagctcg gaggagtacc gcattcagat gagcctcaac
     3001 tacgagggca tcggcgcctc gctgcagctc gtggacgact acgtcaccat catgaatgtg
     3061 ctcgagggcg gacccgcggc agtcgccggg acgctcaaga ccaacgaccg catcgtcggc
     3121 gtcggccagg gccacgatgg gccgttcacg gacgtgatcg gctggcggct cgatgacgtg
     3181 gtgcagctca ttcgcggcaa ggccaacacc atggtgcgcc tgcaggtgct gccggcgggc
     3241 gccgttcccg gcagcgggga aaaggtgctc gagttcgtgc gcaacaaagt gacgctcgaa
     3301 gcgcaggcgg cacacaagga agtgaagagc gtggtgcgca acggccgcac gctcaagatc
     3361 ggcgtcatca ccgtgcccgg cttttatcag gacatcgccg cgcagaatgc cggtgaccag
     3421 aactaccgca gcaccacgcg cgacgtgctg aagctcatca accagctcaa ggccgagaag
     3481 atcgacggcc tggtgctcga cctgcgcaac gatggcggcg gctacctgcc cgaggcgacc
     3541 gcgctcacgg gactgttcat cgatcacggc ccggtggtgc agctgcgcga tacctccggc
     3601 aggctcgagg tgctcgatga tccgcagacc ggccc
//
LOCUS       DATDMP010000427         4079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1068589, whole genome shotgun sequence.
ACCESSION   DATDMP010000427 DATDMP010000000
VERSION     DATDMP010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068589"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /gene="grpE"
                     /locus_tag="VI195_07475"
     CDS             <1..495
                     /gene="grpE"
                     /locus_tag="VI195_07475"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY6824265.1"
                     /translation="ESPPSAVELERLQQALLETDERAKNHYEQYLRAAAELDNVRKRA
                     QRDIEAANRYGLEKFAAELLPVKDSLELAVQNSGRADAKSLREGQEATLQLLSRALEK
                     LGVTMIDPEGEPFDPARHEAMMTQESATAEPNSVLKVVQPGYELNGRLLRPARVIVAR
                     APAE"
     gene            606..2570
                     /gene="dnaK"
                     /locus_tag="VI195_07480"
     CDS             606..2570
                     /gene="dnaK"
                     /locus_tag="VI195_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY6824266.1"
                     /translation="MAKTIGIDLGTTNSCVAIMEGGKPRVIENSEGDRTTPSIVAFSK
                     DGEVLVGQPAKRQAVTNPQNTLFAIKRLIGRKYDDEVVQRDIKMVPYKIVKADNGDAW
                     VEVQGKKMAPPEISARVLMKMKKTAEDYLGEPVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKQGGDRKIAVYDLGGGTFDISIIEIAEVDGERQFEV
                     LSTNGDTFLGGEDFDLRIINFLGEQFLKESGVDVRNDPLAMQRLKEAAEKAKIELSST
                     QQTDINLPYITADASGPKHLNIKLTRAKLESLVEDLVQKTVPPCRTALKDAGVSVGDI
                     AEVILVGGQTRMPLVQKYVKDLFGKEPRKDVNPDEAVAVGAAIQGGVLAGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIEKNTTIPTKASNTFSTADDNQTAVTIHVLQGERERAAD
                     NKSLGKFDLADIPPAPRGMPQIEVSFDIDANGILNVSAKDKATGKEQKIVIKASSGLN
                     EDEIKRMVRDAEAHAEEDKRFRELVDSRNKADALIHAVEKSLKDLGEKVDAAERAKVE
                     SSVSDLRTALKGDDRGVIDKKAEALAQASASIAQRAYAGSQAGPGGPGEPAADASAGG
                     EGGGAGGGSGREDVVDAEFEEVKDKGRKAS"
     gene            2580..3725
                     /gene="dnaJ"
                     /locus_tag="VI195_07485"
     CDS             2580..3725
                     /gene="dnaJ"
                     /locus_tag="VI195_07485"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY6824267.1"
                     /translation="MAKRDYYKVLDVPRGATEAEIKKSYRRLAMKYHPDRNPNDREAE
                     EAFKEAKEACEVLTDPQKRAAYDQYGHAGVEAASRGAGGRGGFGPDTFSDIFGDVFGD
                     IFGGARRSSRSQVYRGADLRYELELELPQAVFGHQVEISLERLAECEVCRGTGAAKGS
                     TPTTCETCGGAGQVRIAQGFFQLQQTCPRCRGAGTVVRNPCDACLGQGRVRRSRTLAV
                     KVPPGVDTGDRVRLAGEGEAGRNGGPPGDLYVEIHVREHPIFERDGVHLSCEVPVSFA
                     TAALGGSVSVPTLEGDVALKVPAETQSGRVFRLRDKGVKPVRGGSRGDLFCRVVVETP
                     VHLSAEQRELVRRLDESLQAQAARHAPREKGFLEGVRRFFASGSGKG"
     gene            3732..>4079
                     /locus_tag="VI195_07490"
     CDS             3732..>4079
                     /locus_tag="VI195_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824268.1"
                     /translation="MSTAAAHEPLIRAVIIGASGRMGQALLRAAPAFPRLIITGAITW
                     SGSLALGRDAGEVAGIGHLNLPITSDLSAALAAADVALDFSHPAATASTLDACRAARK
                     PLLLGTTGFGAQLD"
BASE COUNT          779 a         1322 c         1389 g          589 t
ORIGIN      
        1 gagagccccc ctagcgcggt cgagctggag cggctgcagc aggccctcct cgagaccgac
       61 gagcgcgcca agaaccacta cgagcaatac ctgcgcgcgg cggcggagct cgataacgtc
      121 cgcaagcgcg cccagcggga catcgaggcg gcgaaccgct atgggctcga gaagttcgcc
      181 gcggagctgc tcccggtgaa ggatagcctc gagcttgcgg tgcagaactc gggccgcgcc
      241 gatgcgaaga gcctgcgcga aggacaggag gcgacactgc agctgctatc ccgggcgctc
      301 gagaagctcg gcgttaccat gatcgacccc gaaggtgagc ccttcgaccc cgcgcggcac
      361 gaggcgatga tgacccagga atcggccacg gcagagccga actcggtcct gaaggtggtc
      421 cagcccgggt acgagctcaa cggccgcctg ctgcgcccgg cgcgggtgat cgtggcccgg
      481 gcaccggccg aatgaggact gaggggcgcg gaggcggttt cgggcttgaa agcgctcatg
      541 gctaccccta agaagtgacc cggacatccc caattagacc tacagaaact gcggcggagt
      601 gacccatggc taagacaatc ggcattgacc tcggcacgac caattcgtgc gtggccatca
      661 tggagggcgg caagcctcgc gtgatcgaga acagtgaggg cgaccgcacc accccctcga
      721 tcgtcgcctt ctccaaggac ggcgaggttc tggtgggcca gcccgccaag cgccaggcgg
      781 tcaccaaccc gcagaacacg ctcttcgcca tcaagcgcct catcggccgc aagtacgacg
      841 acgaggtggt gcagcgggac atcaagatgg tcccttacaa gatcgtcaag gccgataacg
      901 gcgacgcctg ggtagaggta cagggcaaga agatggcacc cccggagatc tccgcgcggg
      961 tgctcatgaa gatgaagaag actgccgaag actacctcgg cgagccggtg aaagaggccg
     1021 tcatcacggt cccggcatat ttcaacgatt cccagcgcca ggcgaccaag gacgccgggc
     1081 gtattgccgg cctggaggtc aagcgcatca tcaacgagcc gaccgcggcg gcgctcgcct
     1141 acgggctcga caagcagggt ggcgatcgca agatcgccgt atacgacctc ggcggcggca
     1201 ccttcgacat ctccatcatc gagatcgcag aggtcgatgg cgagcggcag ttcgaggtgc
     1261 tctcgaccaa tggcgacacc ttcctcgggg gcgaggactt cgacctgcgc atcatcaatt
     1321 ttctgggcga gcagttcctg aaggagtccg gcgtcgatgt gcgcaacgac ccgctcgcca
     1381 tgcagcgtct gaaggaggcc gccgagaagg cgaagatcga gctgtcctcg acgcagcaga
     1441 ccgacatcaa tctgccctac atcaccgcgg acgcctccgg tcccaagcat ctcaacatca
     1501 agttgacgcg agccaagctc gaatccctgg tggaggacct ggttcagaag accgtcccgc
     1561 cgtgccggac cgcgctcaag gacgccgggg tgagcgtcgg cgacatcgcc gaggtcattc
     1621 tcgtaggcgg ccagacgcgc atgccgctcg tccagaagta cgtgaaggat ctctttggca
     1681 aggagccacg caaggacgtc aatccggatg aggccgtcgc agtgggcgcc gccatccagg
     1741 gcggcgtgct cgccggtgaa gtgaaggatg tgctgctgct cgatgtgacg ccgctttcgc
     1801 tcggcatcga gaccctcggt ggggtgatga ccaagctcat cgagaagaac accacaattc
     1861 cgaccaaggc atcgaatacg ttctcgaccg cggacgacaa ccagacggcc gtcaccatcc
     1921 acgtgctgca gggcgagcgc gagcgtgccg ccgacaacaa gtcgctcggc aagttcgacc
     1981 tcgccgacat accgcccgca ccgcgcggca tgccccagat cgaagtgtcc ttcgacatcg
     2041 acgccaacgg cattctgaac gtatcggcga aggacaaagc gaccggcaag gagcagaaga
     2101 tcgtcatcaa ggcctcgagc gggctcaacg aggacgagat caaacgcatg gtgcgcgatg
     2161 ccgaggcaca cgcggaggag gacaagcgct tccgcgagct ggtcgactcg cgtaacaagg
     2221 cggacgccct gattcacgcc gtcgagaagt ccctcaagga tctcggcgag aaggtggacg
     2281 cggccgagcg cgccaaggtc gaaagctccg tttccgacct gcgcaccgcg ctgaagggcg
     2341 atgaccgcgg ggtcatcgac aagaaagccg aggctctcgc ccaggcttct gccagcatcg
     2401 cgcagcgggc ttatgcgggc tcccaggcgg ggcctggcgg acccggtgag ccggcggccg
     2461 acgccagcgc aggcggggag ggcggcggcg caggcggggg gtcgggccgc gaggacgtgg
     2521 tcgacgcgga attcgaggag gtcaaggaca aaggacgcaa ggcctcttaa gcttggtcca
     2581 tggcgaagcg cgactactac aaggtactcg acgtaccgag gggggcgact gaggcggaga
     2641 tcaaaaagtc ctaccgccgt ctcgccatga aatatcaccc cgatcgcaac cccaacgatc
     2701 gggaggccga agaagcattc aaggaggcca aggaggcctg cgaggtgctc accgacccgc
     2761 agaagcgggc ggcctacgac cagtacggtc acgcgggtgt cgaggcggct tcgcgtggcg
     2821 ccggcggccg gggcggattc ggccccgaca ccttcagcga catctttgga gatgtgttcg
     2881 gcgacatctt cggtggtgcg cgccgcagca gccgctccca ggtgtatcgc ggcgccgatc
     2941 tgcgctacga gctcgagctc gaactcccgc aggccgtctt cggccaccaa gtggagatca
     3001 gtctcgagcg gctcgccgag tgcgaggtgt gccggggcac gggcgccgcg aaaggcagca
     3061 ctcccacgac ctgtgagacc tgtggcgggg caggtcaggt acgcatcgct cagggcttct
     3121 tccagttaca gcagacctgc ccgcgctgcc gcggcgccgg cacggtcgtg cgcaatcctt
     3181 gtgacgcctg cctcggccag ggccgtgtgc ggcgcagccg cactcttgcg gtcaaagtgc
     3241 cgccgggtgt cgacaccggt gaccgggtgc ggctcgcggg agaaggcgag gcggggcgca
     3301 acggcggacc gcccggtgac ctgtacgtcg agattcacgt ccgcgagcat ccgatcttcg
     3361 agcgcgacgg cgtgcacctc agctgcgagg tgccggtgag cttcgccacc gccgcactcg
     3421 ggggcagcgt gagcgtgccg accctcgaag gggatgtcgc gctcaaggta cccgcagaaa
     3481 ctcagtcggg ccgcgtgttc cgcctgcgtg acaagggtgt gaagccggtg cgcggcggct
     3541 cacgcggcga tctgttctgt cgcgtggtgg tggagactcc ggtgcacctg tccgccgagc
     3601 agcgcgagct cgtacgccgg ctcgatgagt cgctgcaggc ccaggccgcg cgccatgcgc
     3661 cgcgcgagaa aggattcctg gaaggagtgc gccgcttctt tgcctccgga tccggcaagg
     3721 gctgattccg cgtgagcacc gccgctgcgc atgagccgct catccgtgcg gtcatcattg
     3781 gagcgagcgg gcgcatggga caggcgctgc tacgcgctgc ccccgccttc ccccgcctca
     3841 tcatcacggg tgccatcact tggagcggaa gccttgcgct cgggcgtgac gcgggcgagg
     3901 tggccgggat cgggcacctg aaccttccca tcacgagcga cctctcagcc gcgctcgccg
     3961 ctgcagacgt ggcgctcgat ttcagtcatc cggccgcaac ggcgtccacc ctggacgcgt
     4021 gccgcgcggc gcgcaagccg ctgctgctcg gcacgaccgg ttttggcgcg cagctcgat
//
LOCUS       DATDMP010000428         3789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_291740, whole genome shotgun sequence.
ACCESSION   DATDMP010000428 DATDMP010000000
VERSION     DATDMP010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291740"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VI195_07495"
     CDS             complement(<1..232)
                     /locus_tag="VI195_07495"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY6824269.1"
                     /translation="MSETVTPARRGRDSRRARAQSRAVKYPYIRRRLQPVELLSSEAV
                     EIIEANAETILEEVGIEFRRDPESLRLWREAGA"
     gene            395..1543
                     /locus_tag="VI195_07500"
     CDS             395..1543
                     /locus_tag="VI195_07500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6824270.1"
                     /translation="MHLVDATLFYSPTSGGVKRYLSAKHAWLAAHTSWEHTIVVPGRD
                     THFERGGVCTLSGYPVPGTFNYRLPLNPRRWTRLLDTLEPTLIEAGDVFHPAWAGWLV
                     ARRRGAPLVGFYHSNLPQLAGRRACGWWTEPVLRRYVRLLYDRCDLVFAPSRLMCEYL
                     QSLGVAQVVHQPLGVDAEVFNPARRSDGLRECLGLPAHARVLVYAGRFAEEKNLPVLL
                     QAFAHLGTPYHLLLIGGARRARPAANVTMLPYRRDSHELARWIASADALVHAGTKETF
                     GLVILEAMACGRPVVAARAGAFPEFVDDTVGVLAAPHSAAAMADAIAALYERDIAALG
                     AAARARVLRHFTWSRAFHAQLAAYASLLGTQRLPLPDTPILELRSGSS"
     gene            complement(1580..2008)
                     /locus_tag="VI195_07505"
     CDS             complement(1580..2008)
                     /locus_tag="VI195_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824271.1"
                     /translation="MRTEVNIIEPVSMPERLASFRGEVHCERAQRVPGLTLVGNTHEA
                     PAELSALAFSGSAPAELPATLADALVEHLGGTQYRISADSRSWLISASAVHLHREIAA
                     QFYAAIPPRRAPWAKRLFWRAVLALAASRMGLTLLKALRR"
     gene            2034..3470
                     /locus_tag="VI195_07510"
     CDS             2034..3470
                     /locus_tag="VI195_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6824272.1"
                     /translation="MSTPDLQPRRDLTLTHATSLVVGIIIGTGIFLKSATVAQAVGTP
                     ALVLAAWSAAGLVAMLGALCFAELGALLPHAGGEYVYLSRAYGEIPGFLFACNAFVLG
                     GAAVAAYGAAVAIFVSDIHPFGEDWMVRSVHLFGGDYRFTFGARQLLAVAVIGVFAGI
                     NCAGVMLGGRVQALLTAAKVLAIVAVALGVFLFSRTGAASNLIAPAGSGNGGLTGFGA
                     ALFSALWAYSGWQFLPMAASEVRAPQRNLPRAIVGGTLLVLVLYLMINTAYLYALPFW
                     QVTSANSTAYPEAPSVAARAAQTFLGSHAAAVAALIFLVSTIGSMNGVILSAARVPYA
                     AARDGLFFERFGRLSRGSRVPVTALVLLALWGALLALSGTFDQLTNMAVMSYALFWIP
                     VALAIPVLRRKLPHEPRPYRVPGYPLVPLLFAAIMAWIVVSALRSAPRESLATLVLIL
                     LGLPLYPLFRRRRARELAAVTSTPAPRA"
     gene            complement(3442..>3789)
                     /locus_tag="VI195_07515"
     CDS             complement(3442..>3789)
                     /locus_tag="VI195_07515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6824273.1"
                     /translation="LCRRYGWLRGRIEEYRTRAPFDLVICYDVLQYLTGAAAQRALAN
                     FGRLCRGALYFSALTRHDYQQNCDQARTDRQVHLRSARWYRTRLRRQFDEAGLGFWLR
                     RGAPLTLWELESK"
BASE COUNT          507 a         1356 c         1283 g          643 t
ORIGIN      
        1 ccgcacctgc ctcgcgccac aaccgcaacg actcggggtc gcgccggaac tcgatcccca
       61 cttcctcgag aatggtctcg gcgttggcct cgatgatctc gaccgcttca gagctcaaca
      121 actccaccgg ctgcaggcgc cggcggatgt aagggtattt gacggccctc gactgcgcgc
      181 gtgcgcggcg gctgtcgcgt ccgcgccgcg cgggggtcac ggtctcgctc atatgtcagg
      241 ccttcctgcg gcgtgcatgc gccgagtcat cttacgctca ccgcattccc aacatcacgc
      301 tgacacgagg ccgagcggtc ctcgcccacg cttgacactc cattaccata aaggtgcaat
      361 gcgctctttg gcgcgtgctg gcgaaacaat aaccgtgcat ctcgtcgacg caaccctgtt
      421 ttattctccg acctcaggcg gcgtgaaacg ctacctgtcg gcgaagcatg cctggctcgc
      481 ggcgcacacc tcctgggaac acaccatcgt cgtaccgggc cgggacacgc acttcgagcg
      541 cggtggggtg tgcacgctgt cgggctaccc ggtaccggga accttcaatt accgcctgcc
      601 gctcaatcca cggcgctgga cgcgcctgct cgatacgctc gagccgacgc tcatcgaggc
      661 gggagatgtg ttccatcccg cctgggccgg ctggctggtg gcgcggcgcc gcggcgcccc
      721 gctggtcggc ttctatcact cgaatctgcc gcagctcgcc gggcggcgcg cgtgcggctg
      781 gtggaccgag ccggtattgc gccgctacgt gcggctgctg tacgaccgct gcgatctggt
      841 gttcgcgccg agccggctga tgtgcgagta cctgcagagt cttggagtcg cgcaggtggt
      901 gcaccagcca ctcggggtcg atgcggaggt cttcaacccc gcgcgccgca gcgacggcct
      961 gcgcgaatgc ctgggtctgc cggcgcacgc acgggtgctg gtctatgcgg gccgcttcgc
     1021 cgaagagaag aatctgccgg tgctgctgca ggcgttcgcc catctcggga caccctatca
     1081 cctgctgctc atcggcggtg cgcgccgcgc gcgcccggcg gcgaatgtca ccatgctgcc
     1141 gtaccggcgc gacagccacg agcttgcgcg gtggattgcc tccgccgatg cgctggtgca
     1201 cgcgggtacc aaggagacgt tcggcctggt gatcctcgag gccatggcgt gtggccgccc
     1261 cgtagtggcg gcgcgtgccg gcgcctttcc ggaattcgtc gacgacaccg tgggagtgct
     1321 ggccgcgccg cacagcgccg ccgccatggc cgacgccatc gccgcgctgt atgagcgcga
     1381 tatcgcggcg ctcggtgctg cggcacgcgc gcgcgtgctg cggcacttca cctggagccg
     1441 cgcgttccac gcgcagctag cggcctacgc atcgctcctt ggcacgcagc gcctgccgct
     1501 ccccgatacg ccgatcctcg agctgcgctc gggaagctcc tgagcgagcg cggcgcacgc
     1561 cgcgccgcaa ccgctgcggt cagcgccgca gcgccttgag cagcgtgagt cccatgcggc
     1621 tcgcggcgag cgccagcact gcacgccaga acaagcgctt cgcccacggc gcgcgccgcg
     1681 gcgggatggc cgcgtagaac tgcgcggcga tctcccggtg cagatgcacc gcggaagcgc
     1741 tgatcagcca gctgcggctg tctgcgctga tgcggtactg cgtacccccg agatgctcga
     1801 ccagggcgtc cgccagcgtc gcgggcagct cggccggggc ggagccgctg aaggcgagcg
     1861 cgctcagttc cgcaggcgcc tcgtgggtgt tgcccacgag cgtgagcccg gggacccgct
     1921 gcgcgcgttc gcaatgcacc tcgccacgaa agctcgcaag ccgctcaggc atcgacacgg
     1981 gctcgatgat gtttacctcg gtgcgcactg caggcgattg tacgatagcg cgcatgagca
     2041 ccccggatct gcagccgcgc cgcgacctga cgctcacgca cgccacttcg ctcgtggtcg
     2101 gaatcatcat cggcaccggc atcttcctca agtccgccac cgtggcccag gcggtcggaa
     2161 ccccggcgct ggtgttggcg gcctggagcg cggcgggcct ggtggcgatg ctcggcgcgc
     2221 tgtgctttgc cgagctcggc gcgttgctgc cgcacgcggg cggagaatac gtctatctca
     2281 gccgcgccta tggcgagatc cccgggttcc tgttcgcctg caacgccttc gtgctgggcg
     2341 gcgccgccgt cgcagcctac ggtgcggcgg tcgccatctt cgtctccgac atacacccct
     2401 tcggtgagga ctggatggtg cgcagcgtgc acctcttcgg cggcgactac cgcttcacct
     2461 tcggcgcgcg gcagctgctc gccgtcgcgg tcatcggcgt attcgccggc atcaactgcg
     2521 ccggggtcat gctcggcgga cgcgtgcagg cgctgctcac cgcggccaag gtgctggcga
     2581 tcgtcgcggt cgcgctcggt gtgtttcttt tcagccgcac cggcgccgcc agcaatctca
     2641 tcgcacccgc cggcagcggc aacggcggcc tcaccggctt cggcgcggcg ctgttttccg
     2701 cgctgtgggc ttacagtggc tggcagttcc tgccgatggc ggcgagcgag gtgcgcgcgc
     2761 cgcagcgcaa cctgccgcgc gccatcgtgg gaggaacact gctcgtgctg gtgctctacc
     2821 tcatgatcaa cacggcctat ctctacgcgc tgcccttctg gcaggtgacg agcgcgaact
     2881 ccaccgccta ccccgaggcg ccctcggtcg ccgcgcgcgc cgcgcagacc tttctcggca
     2941 gccacgccgc agccgtcgcg gcgctgatct tcctggtctc gaccatcggc tcgatgaacg
     3001 gtgtcatcct gagcgcggcg cgcgtgccct acgccgcggc gcgcgatggg ctgttcttcg
     3061 agcgcttcgg gcgcctgagt cgcggcagcc gcgtaccggt caccgcgctc gtgttgctgg
     3121 cgctgtgggg agcgctgctc gcgctctcgg gcaccttcga tcagctcacc aacatggcgg
     3181 tgatgtcgta cgcgctgttc tggattccgg tggcgctagc catcccggtg ctgcgccgca
     3241 agctgccgca cgagccgcga ccgtaccgcg taccgggcta cccgctggtg ccgctgctgt
     3301 ttgcggcaat catggcttgg atcgtggtga gcgctctgcg cagcgcgccg cgcgaatcgc
     3361 tggccacgct ggtgctgata ctgctgggac tgccgctata tccgctcttc cgccggcggc
     3421 gtgcgcgcga gctcgccgcg gtcacttcga ctccagctcc cagagcgtga gcggcgcccc
     3481 gcgtcgcagc cagaagccga gacccgcctc gtcgaactgc cgccgcagcc gcgtgcggta
     3541 ccagcgggcg ctgcgcagat gcacctgccg gtcggtgcgc gcctgatcac agttttgctg
     3601 gtagtcgtgg cgggtgagcg cgctgaaata cagcgcgccg cggcacaggc ggccgaagtt
     3661 ggcgagcgca cgctgcgcgg ccgcgcccgt gaggtactgc agcacgtcgt agcagatcac
     3721 cagatcgaac ggcgcgcggg tgcggtactc ctcgatccgg cccctgagcc agccgtagcg
     3781 ccggcagag
//
LOCUS       DATDMP010000429         2493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1735898, whole genome shotgun sequence.
ACCESSION   DATDMP010000429 DATDMP010000000
VERSION     DATDMP010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735898"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VI195_07520"
     CDS             complement(<1..962)
                     /locus_tag="VI195_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY6824274.1"
                     /translation="MRQGDTVEFHLKNHPDSKMPHNIDLHGVTGPGGGAASSFTAPGH
                     ESQFTFKALNQGIYVYHCATAPVGMHIANGMYGLMLVEPPGGMKPVDHEYYVMQGDFY
                     TVGKYRERGHQDFDMQRAIDERPTYVLFNGSEGSLVGERALKAKVGDRVRLFVGNGGP
                     NLVSSFHVIGEIFDRVRYEGGVHEQENVQTTLIPAGGAAIVEFHLQVPGSYVLVDHSI
                     LRAFNKGALAILKADGTENKAIYSGKEVDAVYLGDRAANLAAVSEARASEATGTLTKE
                     QQIKAGESRFTGTCSVCHQANGEGLPQVFPPLARSDFLMADKPRS"
     gene            complement(1430..1828)
                     /locus_tag="VI195_07525"
     CDS             complement(1430..1828)
                     /locus_tag="VI195_07525"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEY6824275.1"
                     /translation="MASAIVALLATYYTDIRHLHIACVALSGTLFTSRALLRIKGSLL
                     ANHRALRMSSYCIDTTLLVAGILLSLILDQYPFVNGWLTAKLLLLPCYIGLGLVTLRQ
                     ARSGFWCSVAMMSALLVYAAIITVAVTHRT"
     gene            complement(1813..2373)
                     /locus_tag="VI195_07530"
     CDS             complement(1813..2373)
                     /locus_tag="VI195_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824276.1"
                     /translation="MKPPPTALRTASAAPSMVRARPLACTALVLATLAVAMSVAAQQH
                     PPSGDELRSMYCIEVVRAEIDVQHHLISAADAAAASATTPALRQQWIDTSAELLQGLA
                     RLEIVRYRLQTYMLPRIPALDAAALGIAVRQGNADFQESRAAADRCALECTPQPAMNP
                     ACSAACGNESPLGRLSACYKPTWLPQ"
BASE COUNT          448 a          759 c          824 g          462 t
ORIGIN      
        1 gagcgcggct tgtcggccat cagaaaatcc gagcgcgcca ggggcggaaa cacctgtggc
       61 agcccctcgc cattggcctg atggcagacc gagcaggtgc cggtgaaacg cgactccccg
      121 gccttgatct gttgctcctt ggtcaaagtc ccggtggcct cggaggcgcg cgcttcgctg
      181 accgccgcga ggtttgcggc ccggtcaccg aggtacacgg catcgacctc cttgcccgaa
      241 tagatcgctt tgttttccgt cccgtccgcc ttgaggatgg cgagcgcgcc cttgttgaag
      301 gcacgcagga tcgaatggtc gaccagcaca tagctgccgg gaacctgcag gtggaattcc
      361 acgatcgccg cgccacccgc cgggatcagc gtcgtctgca cgttctcctg ctcgtgcacg
      421 cccccttcgt agcgcacccg atcaaaaatc tcgccgatga cgtggaagct gctcaccaga
      481 ttgggtccac cgttaccgac gaatagccgc acccggtcgc cgaccttggc cttgagtgca
      541 cgctcgccga cgagcgagcc ctcggagccg ttgaacagca cataagtggg acgctcatcg
      601 atcgcccgct gcatgtcgaa atcctggtga ccgcgctcgc gatacttacc gaccgtgtag
      661 aaatcgccct gcatcacgta gtactcgtga tcgacgggct tcataccgcc gggaggctcg
      721 accagcatca acccgtacat gccgttggcg atgtgcatgc cgaccggagc cgtagcacag
      781 tgatagacgt aaattccctg gttgagagcc ttgaaggtga attgcgactc atggcccggc
      841 gcggtgaagc tcgaagccgc gccgccaccc ggaccggtga ccccgtgcag gtcgatgttg
      901 tggggcatct tgctgtcggg atgattcttg agatggaatt ccaccgtatc gccctggcgc
      961 acgcggatgt gtagctgacc ccttccgaga tttccttctc cacttcgcgt acctccagct
     1021 ccacgatcac cttcgccggg tgattgcgat ggatgggcgg cggcacgttg ggagggctag
     1081 tgagcgtggc gtggatgggc tcgccttgtg gcggaccgag gtcgcccggg gcggcggcgg
     1141 gcggcttctt gcctgcggcc atgaccatcg ggcacagcgc cagcgtgcac aatgcgctcg
     1201 ccgctatggc tatgaagcgg ttcatattcg ctccttgggt tctcattatc tttgggttgc
     1261 agaatggtgc cggtaggtac gaggcggaat aagagtaagt gcgtagcatg cgtgcgctgc
     1321 cttcggttgg tacggattga cccctgcctg gcggcaggtt gctgcatgcc gctagccggt
     1381 cgtcttggac caaagggcct tggcaggcgc gccgcgcgtg tggaaattat taggtgcgat
     1441 gcgtaacggc aacggtgatg atcgcggcgt agacgagtag cgcgctcatc atcgctacac
     1501 tgcaccaaaa cccgctgcgc gcctgccgca aggtgacgag gccgaggccg atgtagcagg
     1561 gcagcagcag cagcttcgcg gtcagccagc cattgacgaa ggggtactgg tcgaggatca
     1621 ggctcagcag gatcccggca acgagcaacg tcgtgtcgat gcagtatgag ctcatccgaa
     1681 gggctctatg attggcgagc agcgagccct tgatgcgcag cagcgcacga gaggtgaaga
     1741 gcgtgcccga caaagcgacg cacgctatgt gcagatggcg aatgtccgtg tagtaggtcg
     1801 cgaggagcgc cactattgcg gaagccacgt cggcttgtag catgcgctca gtcggccgag
     1861 agggctctcg ttaccacatg ctgcgctgca ggcagggttc atcgctggct ggggagtgca
     1921 ctcgagcgcg caccgatcgg cagcagctcg cgattcctga aaatccgcat ttccctggcg
     1981 cacggcgatt ccaagagctg cggcatcgag cgcgggtatc ctgggcagca tgtaggtctg
     2041 cagccggtaa cgcacgattt cgagccgcgc cagcccctgg aggagctcag cgctggtatc
     2101 gatccattgc tggcgcagtg cgggggtggt tgcgctggcg gccgctgcgt ccgccgcgga
     2161 gatcaggtga tgctgcacat ctatctccgc ccgtacgact tcaatgcaat acatggaacg
     2221 cagctcgtca ccactcgggg gatgctgttg ggcggccaca gacatggcga ccgcaagggt
     2281 cgccaacacg agcgccgtgc atgccagcgg cctggcgcgc accatcgagg gcgcggcgga
     2341 ggcagtacgg agagcggtcg gtggtggctt cacgggaccc gaagtgtgcc accgtagtgc
     2401 ccaacggcgc gcgcgtttat gctcaagcca gcgtcgccgc gcctgaatgc ccccagtgcc
     2461 cgggcagggt agcgtggtat cccatgacgg cga
//
LOCUS       DATDMP010000430         2372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2180913, whole genome shotgun sequence.
ACCESSION   DATDMP010000430 DATDMP010000000
VERSION     DATDMP010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180913"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VI195_07535"
     CDS             complement(<1..385)
                     /locus_tag="VI195_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6824277.1"
                     /translation="MQATLAGIRVVEQGTFITGPCAGMMLADLGADVIKVESPEGDPY
                     RAYQGGQYSPHFQAYNRNKRSIALDMKYADDCELFRSLIREADVYIQNFRPGTAARLN
                     AGVAELQRLNPRLVYCSISGFGSTGP"
     gene            complement(400..1212)
                     /locus_tag="VI195_07540"
     CDS             complement(400..1212)
                     /locus_tag="VI195_07540"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY6824278.1"
                     /translation="MRIGRQQQRSSAISSSNAETILVRGRDLVRDLVGAIGFTEHFWL
                     LVTGTMPSPAQRRVLDATLVAIAEHGLVPSVQAARMTLAAAPEALQGAVAAGILGCGS
                     VVLGSAEAAGRFFEEIQTRAAKEAALEAVAAAVVREYRAAQRPIPGYGHPLHRGGDPR
                     ATRLLQVAGEAGIAGRYVEIATLVERLVPTLLNRPLTLNVSGAIPAVLLDAGYPLLGL
                     KGVPLLARTAGLIAHLVEEQQRGIGFVMSHAAAQAIDYDGEAPPGFKPSDEA"
     gene            1375..2163
                     /locus_tag="VI195_07545"
     CDS             1375..2163
                     /locus_tag="VI195_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824279.1"
                     /translation="MPRREVMGGLAKGLAVIRAFARDHAALSLSEVARSARIPAATAR
                     RCLLTLEELGYVTHSGRAFLLRPKVLELGAAYLESINIEQITKTHLEELARKTADSAA
                     LCVLDGVDIVYVARTSVRTLLRLEAHVGSRFPAHATSMGRVLLAGLGPERLNRYFDNA
                     QFEALTERTVTDPAKLRTVIEDCRRLGYAAVEDELAYGVIALAVPVFDHQHRVVAALN
                     SSSHSRRITKARLVRERLAMLQEASREISADLASVPWLSLSAQL"
BASE COUNT          405 a          804 c          760 g          403 t
ORIGIN      
        1 aggggccggt cgagccgaaa ccgctgatgg agcaatacac cagtcgcgga ttgagcctct
       61 gcaattccgc gacaccggcg ttgagtcgcg cggcggtgcc gggacggaaa ttctgaatgt
      121 agacatcggc ttcacggata aggctgcgga acagctcgca gtcatccgca tacttcatat
      181 cgagcgcgat gctgcgcttg ttgcggttgt aggcctggaa atgcggtgaa tactgcccgc
      241 cctgatacgc ccggtaggga tcgccctcgg ggctctcgac cttgataacg tctgcgccca
      301 gatcggccag catcattccc gcgcacggac cggtgatgaa ggtgccctgc tcgaccacgc
      361 gaatgccggc cagtgtcgct tgcatgagcg agctacctgc taggcctcat cgctcggctt
      421 gaaaccgggc ggtgcctcgc cgtcgtagtc gatcgcctgc gctgctgcgt gcgacatcac
      481 gaacccgatg ccacgctgtt gttcctcgac cagatgcgca atgagcccgg cggtgcgcgc
      541 gagcagcggc acacctttca ggccgagaag cggatagccg gcgtcgagga gcaccgcggg
      601 aattgccccg gagacgttga gcgtcagtgg acggttgagg agtgtcggca cgagccgctc
      661 gacgagcgtg gcgatctcga cgtaacggcc ggcgatgcca gcctcgccgg ccacctgcag
      721 cagccgcgtg gcacgcggat cgccgccgcg gtgcaaggga tggccgtatc ccggaatcgg
      781 acgctgcgct gcgcggtatt cgcgcaccac ggccgctgcg acggcctcga gagccgcctc
      841 tttggcggcg cgcgtctgaa tctcctcgaa aaatcggccc gcagcttccg ccgatccgag
      901 aaccaccgag ccgcacccca gaatcccggc cgccaccgct ccctgcagag cttccggagc
      961 cgcggcgagc gtcatgcgtg ccgcctgcac gctcggcacg agtccgtgct cggcgatcgc
     1021 cacgagcgtt gcatcgagca cgcgccgctg tgcgggcgag ggcatcgttc cggtaacgag
     1081 cagccagaag tgctcggtga agccgatcgc gcccaccagg tcgcgcacca ggtcgcgccc
     1141 gcgcaccagg atagtctcgg cattcgagct cgagatcgcc gagctgcgct gctgctgccg
     1201 accgatcctc aagttggcga acccctgcgc cgtgttcttc gcatagcgaa atagccgtcg
     1261 ataatcgaaa cgatactgaa cccgcgtccg aagaggagtc aagggtgagc gcccggcctt
     1321 ccgggttttc ggatatcgaa atagactgcc ggcgtgccac gacgcaggac aacgttgccc
     1381 cgccgggagg tcatgggggg acttgccaag ggactcgccg tgatccgcgc attcgcgcgt
     1441 gaccatgcgg ccctgagtct cagcgaagtg gcacgcagtg cgcgcatccc tgccgccacc
     1501 gcacgccgtt gcctgctcac cctcgaggag ctcgggtatg tgacgcacag cggccgcgcg
     1561 tttctgctgc gcccgaaggt gctcgagctc ggcgccgcct acctcgagtc gatcaatatc
     1621 gagcagatca ccaagacgca cctcgaggaa ttggcgcgca agacggccga ttccgcagcc
     1681 ctgtgcgtgc tcgatggcgt cgatatcgtc tatgtcgcgc gcacctcggt gcgcacgctg
     1741 ctgcggctgg aggcgcacgt cggcagccgt tttcccgccc acgccacctc catgggtcgg
     1801 gtgctgctgg ccggtctcgg gcccgagcgg ctgaaccgct atttcgacaa cgcgcaattc
     1861 gaagccctta ccgaacgcac cgtaaccgat cctgccaagt tgcggaccgt gatcgaggac
     1921 tgtcggcgcc tcggctatgc agcggtggag gacgagctcg cctacggagt cattgcgctc
     1981 gccgtgccgg ttttcgatca ccaacatcgc gtggtggcgg cgctcaactc ctcgagccat
     2041 tcgcgccgca tcaccaaggc tcgcctggtg cgtgagcgtc tcgccatgct gcaggaggcg
     2101 agccgcgaga tctcggccga cctcgcctcc gttccctggc tgtcactcag cgcgcagctg
     2161 tgaagttgcc gcccggcttc acccttgacg cctccgggcg caggccgtag cctgcaagcg
     2221 aaaactcttt cgctattcga atatccacag cagcatgacc gcgtgcgcct cagcgtcggc
     2281 acgaccaaca cacgctgcgg atctgtttgt cgagctcaac ggcacacggc tgcgctatcg
     2341 cgacgaggga cgcggtccgg cagtgctgct gg
//
LOCUS       DATDMP010000431         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1069682, whole genome shotgun sequence.
ACCESSION   DATDMP010000431 DATDMP010000000
VERSION     DATDMP010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069682"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1306
                     /locus_tag="VI195_07550"
     CDS             65..1306
                     /locus_tag="VI195_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6824280.1"
                     /translation="MGYELLVGLRYLRARRRNRFISINSLVSMIGIAVGVWALIVVLS
                     VMNGFQKEVRTRILGVAAHVQISGLGNRLADWPSIAKIAAQNKHVLATAPFVQAQGML
                     AAGQAVRGAIVRGILPDQEEKVADLGNVMRMGSLEALKPGEFGIVLGIDLARALQLLP
                     GDKVALVAPQGLVTPAGVIPRLKQFTVVGIFEAGIADADSALALVHLKDAQTLYQLGA
                     DVSGVRLKLDDLYAAPAVAQDLRETLPAGVQAVDWTRTHYNFFRAVAIEKRMMFIILA
                     LIVLVAAINIISTLVVAVKDKQSDIAILRTLGAHPRSVMGIFIVQGMLMGVIGTVIGV
                     ALGIVTALNIDVIVPAIEAVLGFKFLDKGVYLIPELPSDLQMADVISISLMALVLSFV
                     ATLYPSWSAARLNPAEALRYE"
     gene            1299..2000
                     /locus_tag="VI195_07555"
     CDS             1299..2000
                     /locus_tag="VI195_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6824281.1"
                     /translation="MNERQAVVACHALTKTYQGPQPVPVLLGIDLEINRGEHIAIMGR
                     SGSGKSTLLQLLGGLDAPTSGQVFVQGRPFSEMREAERAQTRNATLGFVYQFHHLLPE
                     FSAVENVAMPLFIRRLERADALERAKRVLGEVGLGARLDHLPGELSGGERQRCAFARA
                     LVTEPACVLADEPTGNLDSHTAEEVFEAMLRLSASHGTAFVIVTHDPGLAGRAERVLQ
                     LRDGLLQARNLLPAA"
BASE COUNT          302 a          691 c          678 g          354 t
ORIGIN      
        1 cgcgtatcag gcgcgcgagc agcgcacggg tttcaacgtc catggtgtat tctcgcgccg
       61 atccatggga tatgagctcc tcgtcggcct gcgctatctg cgcgcccggc gccgtaaccg
      121 tttcatcagc atcaattccc tcgtgtcgat gatcggcatc gcggtcggcg tctgggcgct
      181 aatcgtggtg ctgtcggtga tgaacggctt ccagaaggag gtgcgcacgc gcatcctcgg
      241 cgtcgccgcg cacgtgcaga tcagcggcct cggcaatcgc ctcgccgact ggccgtcgat
      301 cgcgaagatc gccgcacaga acaagcatgt gctggcgacc gcgccgttcg tgcaggcgca
      361 gggcatgctg gccgcggggc aggcggtgcg cggtgccatc gtgcgcggca tcctccccga
      421 tcaggaggaa aaggtcgccg acctcggcaa cgtgatgcgc atgggctccc tcgaggcgct
      481 caagccgggt gagttcggca tcgtgctcgg catcgatctt gcgcgggcgc tgcagctcct
      541 gccgggcgac aaggtggcgc tggtcgcgcc gcagggcctg gtgacccccg ccggcgtgat
      601 accgcgtctc aagcagttta cggtggtcgg gatcttcgag gcgggcatcg ccgatgccga
      661 ctcggcactc gcgctcgtgc atctcaagga cgcgcagacg ctgtaccagc tcggcgccga
      721 tgtgagcggt gtgcgcctga agctcgacga tctctatgcc gcgcccgcgg tcgcgcagga
      781 cctgcgcgag acgctgccgg cgggcgtcca ggccgtcgac tggacgcgta cccactacaa
      841 cttcttccgc gccgtggcga tcgagaagcg catgatgttc atcatcctcg cgctgatcgt
      901 gctggtagcg gcgatcaaca tcatctcgac cctggtcgtg gcggtgaagg acaagcagtc
      961 ggatatcgcc atcctgcgca cgctcggcgc gcacccgcgc tcggtcatgg ggatcttcat
     1021 cgtgcaaggc atgctgatgg gcgtgatcgg caccgtgatc ggggtggcgc tcggcattgt
     1081 cactgcgctc aacatcgacg tcatcgtccc ggcgatcgaa gccgtgctcg gcttcaagtt
     1141 cctggacaag ggcgtgtacc tgatcccgga gctgccctcc gacctgcaga tggcggacgt
     1201 gatctcgatc agcctgatgg cgctcgtgct gtcattcgtc gccacgctct atccgagctg
     1261 gagcgccgcg cgcctcaatc cggccgaggc actgcgctat gaatgagcgc caggcggtcg
     1321 tcgcctgcca tgccctgacc aagacctacc agggaccgca gccggtgccg gtcctgctcg
     1381 gcatcgacct cgagatcaat cgcggcgagc acatcgccat catgggtcgc tcgggatcgg
     1441 gcaagtcgac gctcctgcag ctgctcggcg gtctggacgc gccaacctcg gggcaggtgt
     1501 tcgtgcaggg ccgccccttc agcgagatgc gcgaagccga gcgcgcgcag acgcgcaacg
     1561 ccacgctcgg cttcgtctat cagttccatc acctgctgcc ggagttcagc gccgtcgaga
     1621 acgtcgccat gccgctcttc atccggcggc tggagcgcgc ggacgcgctc gagcgcgcga
     1681 agcgcgtgct gggcgaggtc ggtctcggcg cgcgcctcga tcacctgccg ggcgagctct
     1741 ccggcggcga gcgccagcgc tgcgccttcg cgcgcgcgct ggtcaccgag ccggcgtgcg
     1801 tgctcgccga cgagccgacc ggcaacctcg attcgcatac cgccgaggaa gtgttcgagg
     1861 cgatgctgcg tctctccgcc tcccacggca ccgccttcgt catcgtcacg cacgacccgg
     1921 gtttggcggg gcgtgccgag cgcgtgctgc agctgcgcga cggtctcctc caggcgcgca
     1981 atctgctgcc agcggcgtaa cctcgaaaca tttccggaat ggacc
//
LOCUS       DATDMP010000432         4103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2181254, whole genome shotgun sequence.
ACCESSION   DATDMP010000432 DATDMP010000000
VERSION     DATDMP010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181254"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /gene="priA"
                     /locus_tag="VI195_07560"
     CDS             complement(<1..1509)
                     /gene="priA"
                     /locus_tag="VI195_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY6824282.1"
                     /translation="MSHIFRVALDTPVRRLFDYLPPVQATVQPAPGVRVRVPFGRQRR
                     VGVITEVAGDSEVPPARLKPILEVLDATPVLDAALQELLRWAADYYHHPLGEVLASAL
                     PRALRLGAPGRAREERWLSTDTGSAALAAGEPRRAPKQRALLAFLLEQRVAGAATLDE
                     RFGAWRAAARALIARGWIASVEVDVEPPPAPATAPCAAAPTLLPEQSEAVATIVAARE
                     RFGTFVLHGITGSGKTEVYLRVIEQVLRAGRRALVLVPEIGLTPQLLERFRARLQAPL
                     ALLHSGLTDQERLSAWRDAFSGRARIVLGTRSAVFAPLTELGVIVVDEEHDASFKQHE
                     GGFRYSARDLAVMRARHAAVPVVLGSATPALETLHNVLTGRYTRLTLARRAAQAQPPT
                     LRLIDLRSHAVRGGLATPAVLAIERHLAEDGQVLVFLNRRGYAPTLLCTGCGWLAPCR
                     ECDARLTVHLAAGRLRCHHCGADLPLPPRCPQCGFPVKPVGQGTERVEETLGT"
     gene            1628..2215
                     /locus_tag="VI195_07565"
     CDS             1628..2215
                     /locus_tag="VI195_07565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6824283.1"
                     /translation="MLAALHGDVDLTDEAKTRSRALNQFLAGVELKAFKIAQTALRHE
                     DDALDAVQDAMLQLARAYADRPAQEWKPLFYRILENRIRDMQRRRTVRGRVIAWLPFR
                     GEEDEDEVDPIAQAPSPEPQPVRRLELDEAVGALEKALGELPRRQQQAFLLRTLEGLD
                     VAATAAAMGCSEGSVKTHYFRAVQALRAQLGDLYL"
     gene            2221..2610
                     /locus_tag="VI195_07570"
     CDS             2221..2610
                     /locus_tag="VI195_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824284.1"
                     /translation="MTSPTTEFERNARAVLEASIARIDARTRSRLNQARQSALDTVGR
                     RNRPWWRSFALMPAGAVAAALLLAVMLWHRTPGVHEAPLLEGHVSAVEDADLLADAEG
                     LDLIEGWDGPFYEWAADQSDANVQSES"
     gene            2610..2903
                     /locus_tag="VI195_07575"
     CDS             2610..2903
                     /locus_tag="VI195_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824285.1"
                     /translation="MWLARPTVAVLAVLAVLALGGAAARADDVHQSAEEPDIGFLEFL
                     GSVDRLSDVMPDYLSQAESRAKTASDTAPAPPAAPRPVPPPNTPNGPGGHNNG"
     gene            2896..3483
                     /locus_tag="VI195_07580"
     CDS             2896..3483
                     /locus_tag="VI195_07580"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY6824286.1"
                     /translation="MDKHRRPLAMLIAVAALTAAPCLAQQPPPSAPAEPAASAAPQPG
                     MGWSRLSPEQQRLLSGYGAQWNTLSPERQQALARGSERWLGMSEDQRNQAQQRFSRWR
                     SLPPDERQSLRSRWQRFQSLPPGEQATVRENFRRFQQLPPERRQMLRERWHNASPAQR
                     QQMIEHAREMRQAHPSAHSPAAHGGAVHSGPPPHH"
     gene            complement(3488..>4103)
                     /locus_tag="VI195_07585"
     CDS             complement(3488..>4103)
                     /locus_tag="VI195_07585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6824287.1"
                     /translation="VQWGDPDGKKSVGAAHRTLPAEFERPLRGLSVTRLPDPDTYAAE
                     VGFVDDFPVAAEPANGRAWLVHCYGMVGAGRDTDVDSGGGTELYVVIGQAPRHLDRNV
                     TLLGRVVSGMERLAVMPRGTGPLGRYEHVEQYVPITSMRVAADVPPAQRTNLEVLRTD
                     TPTFLAYLEARRNRHEEWFKVPAGHVDVCNMPVPVRAAPGGGTK"
BASE COUNT          626 a         1414 c         1464 g          599 t
ORIGIN      
        1 cgtgccgagc gtctcctcga cgcgctcggt gccctggccg accggtttca ctgggaagcc
       61 gcactgcggg cagcgcggcg gcagcggcag gtcggcgccg cagtgatggc agcgcagcct
      121 gccggcagca aggtgcaccg tgagccgtgc gtcgcactcg cggcacggtg cgagccagcc
      181 gcacccggtg cacagcagcg tcggtgcgta gccgcggcgg ttgaggaaca cgagcacctg
      241 cccgtcctcg gcgagatgac gctcgatggc gagcaccgcc ggcgtggcga gcccgccgcg
      301 caccgcgtgg ctgcgcaggt cgatgagtct cagggtcggc ggctgggcct gcgccgcgcg
      361 ccgtgcgagc gtgaggcgcg tgtagcgtcc ggtgagcaca ttgtgcagcg tctcgagcgc
      421 cggtgttgcc gagcccagca ccaccggtac ggcggcgtgc cgggcacgca tcaccgcgag
      481 atcgcgcgcc gagtagcgga agccaccctc atgctgcttg aaggaggcat cgtgctcctc
      541 atccaccacg atcactccga gctcggtaag cggagcgaat accgccgagc gcgtgccgag
      601 cacgatgcgc gcgcgcccgc tgaaggcatc gcgccacgcg ctgaggcgct cctgatcggt
      661 caatcccgag tgcagcagcg ccagcggcgc ctgcaagcgt gcccgaaacc gctcgagcag
      721 ctgcggcgtc aagccgatct cggggaccag caccagcgcg cgccggccgg cgcgcagcac
      781 ctgctcgatg acccgcaggt acacctcggt cttgccactg ccggtaatgc cgtggagcac
      841 gaatgtgccg aagcgctcgc gcgcagccac gatggtggct accgcctcgc tctgctccgg
      901 caacagtgtt ggtgcggcag cgcacggcgc cgtggcgggc gccggcggtg gctcgacatc
      961 cacctcgacg ctcgcgatcc agccgcgtgc gatgagtgcc cgtgccgccg cgcgccaggc
     1021 accgaagcgc tcatcgagcg tcgccgctcc tgccacacgc tgctcgagaa ggaaagcgag
     1081 cagtgcccgc tgcttcgggg cgcgccgcgg ctcgcccgcg gcgagcgccg cgctgccggt
     1141 gtcggtcgag agccaacgct cctcccgcgc gcgtcccggg gcgccgaggc gcagcgcccg
     1201 cgggagggcg ctcgccagga cttctccaag ggggtggtga tagtagtcgg cggcccagcg
     1261 cagcagttcc tgcagtgcgg catcgagcac gggcgtggca tcgagcactt cgaggatggg
     1321 cttgagccgt gccggcggaa cctccgagtc gccggcgacc tccgttatga caccgacccg
     1381 gcgctgccgg ccgaatggga cccgcacgcg taccccgggc gcgggctgca cggtcgcctg
     1441 caccggggga agatagtcga acaaccggcg cacgggggtg tccagggcca cccggaagat
     1501 gtgggacaca gtttgttaga ttagctagat cagtggctta tgcggctcaa cagcggtcaa
     1561 ccccggcacg ccagcgcgcg ttatacaggc catgtccgag tttgctacgc tcgcgtcgcc
     1621 ttatagcatg ctggccgctt tacacggcga cgtggacctg acggacgaag ccaagacacg
     1681 ctcccgtgcg ctgaaccaat ttctggccgg cgtggagctc aaggcattca agatcgccca
     1741 gacggcgctg cggcacgagg acgacgcgct tgatgcggta caggatgcca tgctgcagct
     1801 cgcgcgggcc tatgcggacc gtccggcgca ggagtggaag cccctcttct atcgcattct
     1861 cgagaaccgc atccgcgaca tgcagcgccg gcgcacggtg cgcggccgtg tgatcgcgtg
     1921 gctgccattc cggggcgagg aggatgagga cgaggtggac ccgatcgcgc aggctccgag
     1981 ccctgagccg cagccggtga ggcggctgga gctcgatgag gcggtcggtg ccctggagaa
     2041 agcgctcggt gagctgccgc gccggcagca gcaggctttt ctgctgcgca cgctcgaggg
     2101 gctcgacgtg gccgccacgg cggcggcgat gggctgctcc gaaggcagcg tcaagacaca
     2161 ttattttcgc gcggtgcagg cactgcgcgc gcagcttggg gacctttact tatgaggccg
     2221 gtgacttcgc cgacgaccga atttgagcgc aacgcgcgtg cggtgctcga ggcgagcatc
     2281 gcgcgcatcg atgcgcgcac gcgctcacgg ctgaaccagg cgaggcagtc ggcgctcgat
     2341 accgtaggcc ggcgcaatcg tccgtggtgg cgatcgtttg cgctcatgcc ggcgggtgcc
     2401 gtggcagcgg cgctgctgct cgcggtgatg ctctggcacc gcacgccagg cgtgcacgag
     2461 gcaccgctgc tcgagggtca tgtcagcgcc gtcgaggacg cggatctgct tgcggacgcc
     2521 gaaggactgg acctcatcga gggatgggat ggtccgttct acgagtgggc ggccgatcag
     2581 agcgatgcca acgtgcagag cgaaagctga tgtggcttgc acggccgact gtcgcggtcc
     2641 tggcggtact ggcagtgctc gcgctcggcg gcgccgctgc ccgtgccgac gacgtgcacc
     2701 agtcggcgga ggagcccgat atcggcttcc tcgagtttct cggcagcgtc gaccggttgt
     2761 cggatgtgat gccggactat ctctcgcagg cggagtcgcg cgccaagacc gcgtccgaca
     2821 ccgcgcccgc gccgcccgcg gcgccgcggc cggtaccgcc gccgaatacg ccgaacggcc
     2881 ccggaggaca taacaatgga taagcaccgg cggccgctgg ccatgctcat cgcggtggct
     2941 gcgctgaccg ctgccccgtg tcttgcgcag caacccccgc catcggcgcc cgccgagccg
     3001 gcggcgtccg cggctccgca gccgggaatg ggctggtccc gcctgtcacc ggagcagcag
     3061 cggctcctgt cgggttacgg cgcacagtgg aacacgttgt cgccggagcg ccagcaggcg
     3121 ctcgcacgcg ggagcgagcg ctggctcggc atgtcggagg atcagcgtaa ccaggcgcag
     3181 cagcgcttct cgcgctggcg gtcgctgccg ccggacgagc gccagtcgtt gcgctcgcgc
     3241 tggcagagat ttcagtcgct gccgcccggg gagcaggcga cggtgcgcga gaatttccgc
     3301 cgttttcagc agctgccacc cgagcgccgc cagatgctgc gtgagcgctg gcacaacgca
     3361 agtcccgctc agcgtcagca gatgatcgag cacgcgcgcg agatgcgcca ggcgcacccg
     3421 agcgcgcatt cccccgcggc ccacggcggc gccgtgcaca gcgggccacc tccgcaccac
     3481 tgagacgcta tttcgtgccg ccacccgggg ccgcgcgcac cggcacgggc atgttgcaga
     3541 catcgacgtg tcccgcgggc accttgaacc actcctcgtg tcgattgcgg cgtgcctcga
     3601 ggtaggcgag aaaggtaggt gtatcggtgc gcagcacttc gaggttggtg cgctgtgccg
     3661 gcggcacgtc cgcggccacg cgcatggagg tgatcggcac gtactgctcc acgtgctcgt
     3721 agcggcctag cggtccggtg cctcggggca tcacggcgag gcgctccatg ccagacacca
     3781 cgcgccccag gagcgtcacg ttacgatcga ggtggcgtgg cgcctgcccg atcaccacat
     3841 acagctcggt gccgccgccg ctgtcgacat cggtgtcgcg tccggcacct accatgccgt
     3901 agcagtgtac gagccaggca cggccgttcg ccggctcggc cgctaccggg aagtcatcca
     3961 cgaagccgac ctcggcggca taagtgtcgg ggtccggcag ccgggtcact gacagcccgc
     4021 gcagcggacg ctcgaactcc gcaggcagtg tccggtgcgc cgcgccgacg gacttctttc
     4081 cgtcggggtc tccccattgc act
//
LOCUS       DATDMP010000433         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1069887, whole genome shotgun sequence.
ACCESSION   DATDMP010000433 DATDMP010000000
VERSION     DATDMP010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069887"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VI195_07590"
     CDS             <1..1295
                     /locus_tag="VI195_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY6824288.1"
                     /translation="WAALPCFAEDLLQVYRDAQRYDAQYSAARYALEAGREKLAQGRA
                     LVLPTLGLTGNLTRQRIDSVTHDPALAPSFLRYPRAFGYQLTFTQPIFRLQNWLQYDE
                     GGVQVQQAEATFGQAAQDLMLRVAQAYFDVLAAEDTLEVVRTQKTAISEQLAQAKRNF
                     EVGTATITDTHEAQARADLIHAQEIAAQNDLDSRRRSLQLITGKEYGVLKHLRPDVKL
                     SQPNPNDMQSWVDLAEKQSYPVLVQRATTEIASLEARRNRAAHLPTLDLVGTHGTTNQ
                     TGTLTSGVGNDLTVTSVGVQLAFPLYQGGALSSRDREAAALALKAKDDLENARRTSAL
                     TTRQSYLSVINGIAQVGALEQALVSSQSALESNKLGYEVGVRINIDVLNAQQQLFSTQ
                     RDLAVARYNTITNHLRLKAAAGSLIDQDLEEVNRALQP"
     gene            complement(1302..>2316)
                     /locus_tag="VI195_07595"
     CDS             complement(1302..>2316)
                     /locus_tag="VI195_07595"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY6824289.1"
                     /translation="LANYTVLLTCTTASGRAMAKQVYGESVLCAYLPYDYPESVQRFL
                     ERFRPRLGILMETEIWPNLIAECKALAVPLVLANARMSEKSAAGYERWHALFWPAFAS
                     LAGVCAQSEADAIRLRGLGASAVEVCGNLKFDVQVDEAQLERGRLWRRQLERPVLLLA
                     STREGEEDLLLGALPEWDGKLLVIVVPRHPQRFDALARIADARRSRDDFPGPKQRIYL
                     GDSMGEMGFYYGVCDVAVIGGSFAPLGGQNLIEALAAGAAVVTGPSMYNFAEATRLAV
                     EAGAVVQGEDAGTAVRLALELLGNSQKRQAMAAAGKALCEANRGATARHLEVCLNLVK
                     TGA"
BASE COUNT          417 a          842 c          737 g          320 t
ORIGIN      
        1 cgtgggccgc gctgccgtgc ttcgcggaag acctccttca ggtctatcgc gatgcgcagc
       61 gctacgacgc ccagtactcg gctgcgcgct acgcgctgga ggccggccgc gaaaagctgg
      121 cgcagggacg cgcgctcgtc ctgccgaccc tcgggctcac cggcaacctc acgcgccagc
      181 gcatcgattc ggtcacgcac gacccggcac tcgcgccgag cttcctgcgc tatccgcgcg
      241 cgttcggcta ccagctcact ttcacgcagc ccatcttccg cttgcagaac tggctgcagt
      301 acgacgaagg cggggtccag gtccagcaag ccgaggcgac attcggccag gccgcgcaag
      361 acctgatgct gcgcgtcgcg caggcgtact tcgacgtcct cgctgcggag gacacgctgg
      421 aagtggtacg cacgcagaag accgcgatct ccgagcagct cgcccaggcg aagcgcaact
      481 tcgaggtggg caccgccacc atcaccgaca cgcacgaggc gcaggctcgg gccgacctga
      541 ttcacgcgca ggaaatcgcc gcgcagaacg atctcgacag ccgccgccgc agcctgcagc
      601 tcatcaccgg caaggaatac ggggtgttga agcatctgcg ccccgacgtg aagctctcgc
      661 agcccaaccc gaacgacatg caaagctggg tggacctcgc cgagaagcag agctatcccg
      721 tgctcgtgca gcgcgccacg accgagattg caagcctgga ggccaggcga aaccgcgcgg
      781 cgcacctgcc gaccctcgac ctcgtcggca cgcacggcac gacgaaccag accggcacgc
      841 tcacttccgg ggtcggcaac gatctcaccg tcacgagcgt gggcgtgcag ctcgcgtttc
      901 cgctctacca gggcggcgcg ctcagctcgc gcgaccgcga agcggcggcg ctggcgctca
      961 aggcgaagga tgacttggag aacgcgcgca ggacctcggc gctgaccacg cggcagagct
     1021 acctctcggt catcaacggc atcgcgcagg tgggcgcgct cgagcaggcg ctggtgtcct
     1081 cgcagagcgc gctggaatcg aacaagctcg gctacgaagt cggcgtgcgc atcaacatcg
     1141 atgtgctgaa cgcccagcag cagctcttct ccacgcagcg cgacctcgcc gttgcacgct
     1201 acaacaccat caccaaccac cttcgcctca aggcggccgc gggcagcctg atcgaccagg
     1261 acctcgagga agtgaaccgc gcgctgcagc cctaatcggg gtcaggcacc ggttttcacc
     1321 aagttcaggc agacctccag atggcgcgcc gtggcgccgc gattcgcctc gcaaagcgct
     1381 ttcccggcgg cagccatcgc ctggcgcttc tgcgagttgc cgagcagttc gagagcgagg
     1441 cggaccgccg tgcccgcatc ttcgccctga accacggccc cggcttcgac cgcaaggcgc
     1501 gtcgcctcgg caaagttgta catgctgggg ccggtcacga ccgcggcgcc cgcggccagc
     1561 gcctcgatga ggttctgccc cccgagcggc gcgaacgagc cgccgatcac ggcgacgtcg
     1621 cagacgccgt aatagaaccc catctccccc atgctgtcgc cgagatagat ccgctgcttc
     1681 gggcccggaa aatcatcccg gctccggcgc gcatccgcga tccgggcgag cgcgtcgaag
     1741 cgctggggat ggcgcggaac cacgatgacg agaagcttgc catcccattc cggcagcgcg
     1801 ccgagcaaga ggtcctcctc gccctcgcgc gtgctcgcga ggagcagcac cgggcgctcg
     1861 agctgccgcc gccacaggcg cccgcgctcg agctgcgcct cgtccacctg cacatcgaac
     1921 ttcaggttgc cgcacacttc aacggcgctt gcgcccaggc cgcgaagccg gatcgcgtcg
     1981 gcctcgcttt gcgcgcacac gcccgcgagg ctcgcgaaag ccggccagaa gagcgcatgc
     2041 cagcgctcgt acccggccgc ggacttctcc gacatgcgcg cattcgcgag gacaagcgga
     2101 accgcgagcg ccttgcactc ggcaatgagg ttcggccaga tctcggtttc catcaggatg
     2161 ccgaggcgcg gccggaagcg ctcgaggaag cgctgcacag actccggata gtcgtagggc
     2221 agataagcgc acagcaccga ctcgccgtac acctgcttgg ccatggcgcg gccgctcgcc
     2281 gtcgtgcagg tcaggagcac cgtgtaattg gccagc
//
LOCUS       DATDMP010000434         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_292515, whole genome shotgun sequence.
ACCESSION   DATDMP010000434 DATDMP010000000
VERSION     DATDMP010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292515"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /gene="rpsG"
                     /locus_tag="VI195_07600"
     CDS             complement(<1..415)
                     /gene="rpsG"
                     /locus_tag="VI195_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY6824290.1"
                     /translation="MSRRAQAPVRTILPDPKYNNEMLAKFMNVVMKNGNKSAAERIIY
                     GALDRIAEKTGKQGGEAIEVLSTALDNVKPVVEVKSRRVGGATSQVPVEVRATRRQTL
                     AMRWMIEAARDRSEKSMAFRLAHELMDASENRGAAV"
     gene            complement(430..804)
                     /gene="rpsL"
                     /locus_tag="VI195_07605"
     CDS             complement(430..804)
                     /gene="rpsL"
                     /locus_tag="VI195_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY6824291.1"
                     /translation="MATTGQLIRQPRRIGAEKTKVPALGASPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGYEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGTL
                     DCAGVGERKQGRSKYGAKRPKK"
     gene            1132..>2178
                     /locus_tag="VI195_07610"
     CDS             1132..>2178
                     /locus_tag="VI195_07610"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEY6824292.1"
                     /translation="MAASLPLAATAVPTLVPDLPSVAQLIPGNGSPLEVRVLEPSQGD
                     PPAGSVTAGVLDGHFVPFVSRGSHLLTAPLWFRLPPLASSPSRDASRIPVLLARAGMD
                     QTVQLFVRHQGESVALVAATVVPQFGGAQDMVFALPPGLDAGQPLYARVTRQGRATTD
                     LHFTASTLERTLQVAATHQRIIALAFGALMAMALSTLLVRFVLTDRLYPLYGTLFSLQ
                     ALYLAYFSGQGFHWPILSLARPISSYAWNLPVAISGAAAALFVREFANLRLFSPKVYT
                     AFGWLAAAFVALALCNVLRVVPGLSPLIAAIGNLMFLGSALFTLIVSYLAWRRGNRAA
                     GWFLIAWALLSTFQI"
BASE COUNT          295 a          736 c          687 g          460 t
ORIGIN      
        1 gcactgcggc gccgcggttc tcggaggcgt ccataagctc gtgcgccagg cgaaaggcca
       61 tggacttctc gctgcggtcg cgagcggcct cgatcatcca gcgcatggcc agtgtctggc
      121 gacgcgtggc ccgaacctca accggtacct gggaggttgc accgccgacg cggcgcgatt
      181 tcacttcgac gacgggtttc acgttatcga gggctgtcga cagcacctca atcgcttcgc
      241 cgccctgctt gccggtcttt tcggcaatgc gatcgagcgc accatagatg atgcgttcgg
      301 cggcggactt gttgccattc ttcatgacga cgttcatgaa cttggcgagc atctcgttgt
      361 tgtatttcgg gtccggaaga atggtgcgca ccggagcctg ggcacgacgg gacatagctt
      421 gctactcctt tatttcttcg ggcgcttcgc gccgtacttg gagcggccct gcttgcgctc
      481 gccgacgcct gcacaatcga gcgtgccgcg taccgtgtga taacgcacgc ccgggaggtc
      541 cttgacgcgg ccgccgcgga tcagcaccac cgagtgctcc tgcaggttgt ggccttcgcc
      601 gccgatgtag ctggtgacct cataaccgtt ggtgagccgg acgcggcaga ccttgcgcag
      661 cgcggaattc ggcttcttcg gcgtcgtggt gtagacgcgc gtgcagacgc cgcgcttctg
      721 cggggacgca cccagcgccg gcaccttggt tttttccgct ccgatccggc gtggctgccg
      781 aatgagctga cctgtggtcg ccatcgtttc ctcgtctcct cgcgcgataa aacgacctgc
      841 ggagaacacc gggccgcctt catgaggcgg cccggttctc gtaagtcgct gtttccgggc
      901 cggcatgtat gggggccgcc cgacaaaggc cggcgattct aggtacggac ccctggagtg
      961 tcaaccgccg ggcaggaccc ctccgccccc cacacatatt ggcttgtcgg cgggtcgctc
     1021 attaggatag tcgccctgaa gcccggttac ggatgatata gaacagggcc tctcgtggtg
     1081 ggctacaggg attcggtgca caagttggcg ttgaggagat gggtggcggc agtggcggct
     1141 tctctgccgc tggccgcgac ggcggtaccc acgctggtcc ctgacctgcc ttcggtcgcg
     1201 cagctcattc cgggaaatgg ctcgccgctg gaagtccgcg tgctcgaacc ttcccagggg
     1261 gatccgccgg ctggcagcgt caccgccggc gtcctggacg gacacttcgt cccctttgtc
     1321 tcgcgcggct cgcacctgct cacggccccg ctatggtttc ggctcccgcc gctcgcctcc
     1381 tcgccctcgc gcgatgcgag ccgtatcccg gtgctgctcg cgcgtgcggg catggaccag
     1441 actgtgcagc tcttcgtgcg gcatcagggt gagagcgtcg cgctcgttgc cgcgacagtc
     1501 gtgccgcagt tcggcggcgc gcaggacatg gtcttcgcgc tgccaccggg tctcgatgcc
     1561 ggccagccgc tctatgcgcg tgtcacccgt cagggccgtg ccaccaccga cctgcacttc
     1621 accgcttcga ccctcgagcg cacgctgcaa gtcgcggcaa cgcatcagcg catcatcgcg
     1681 ctcgcctttg gcgcgctcat ggcgatggcg ctgtcgacgc tgctggtgcg cttcgtgctc
     1741 accgatcgtc tctatccact ctacggcacc ctgttctcgc ttcaagcgct ctacctggcg
     1801 tacttctccg gacagggatt tcactggccg attctgtcac tggcgcgtcc catttcctct
     1861 tacgcctgga atctaccggt ggcgatctca ggagcggcgg cggcgctgtt cgtgcgcgag
     1921 ttcgcgaacc tgcggctgtt ctcaccgaag gtgtacacgg ccttcggctg gctcgccgcc
     1981 gccttcgttg cgctggcgct ctgcaacgtg ctgcgggtgg taccgggact ttcgcccctg
     2041 attgcggcca tcggcaacct catgtttctc ggcagcgcct tgttcacgct catcgtctcc
     2101 tacctcgcct ggcgtagagg caatcgcgcc gcggggtggt tcctcatcgc ctgggcactg
     2161 ctgtccacgt tccagatc
//
LOCUS       DATDMP010000435         5179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_292685, whole genome shotgun sequence.
ACCESSION   DATDMP010000435 DATDMP010000000
VERSION     DATDMP010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292685"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VI195_07615"
     CDS             complement(<1..314)
                     /locus_tag="VI195_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824293.1"
                     /translation="MSISGKTVRNTALLLGLSLAACSTEPTRYENPYSPQSGHQYRYG
                     VMPTRECADKMSTWESAHPFGAPTSDTLAYGGGVDQVGVTSGAPRVYLVFFGSQWAQG
                     GGG"
     gene            complement(361..1713)
                     /gene="mnmE"
                     /locus_tag="VI195_07620"
     CDS             complement(361..1713)
                     /gene="mnmE"
                     /locus_tag="VI195_07620"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY6824294.1"
                     /translation="MTRTQTIVAAATPPGRGGVGIVRVSGPKTPELAAILLGEVPAAR
                     YATFARFLDAQQCPIDAGLALFFPAPHSYTGEHVLELHGHGGPLVVEALVERVLELGA
                     RRALPGEFTQRAFLNDKLDLAQAEAIADLIDAGSRAAARAAMRSLRGEFSAMVRGLTE
                     AVIELRTYVEAAIDFPEEEIDFLADRELGERFQAVREHFAGVLESARIGRLLREGMTV
                     VIAGPPNAGKSSLLNRLAGYDAAIVTSIPGTTRDVLRERIHLDGMPLHILDTAGLREG
                     GDLVEEEGMRRAQAEMQHADRVLFVIDAATDPDGGALRRERARLPADVPLTLLFNKCD
                     LAPGFAVANDPDGPPRFALSALTGAGLAELRSHLKTSMGYQSVDGGAISARRRHLESL
                     ERARTHTEEAERLLTERRAGELVAEELRLAQQALNEITGEFTSDDLLGRIFAGFCIGK
                     "
     gene            complement(1781..3505)
                     /gene="yidC"
                     /locus_tag="VI195_07625"
     CDS             complement(1781..3505)
                     /gene="yidC"
                     /locus_tag="VI195_07625"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY6824295.1"
                     /translation="MNTNRLGNLRVYLWAALAMVLLYDYQLWVHDYAPPPVPASAPAA
                     AAPSSAPGSGALGNRVPEAVSPSTSAPSSAPVPQSAAGATGVAGAAATPEPAAAAPVV
                     HVRTDVLDVDISTRGGTLARVDLLAYPRVKGEAAPVRLQNQDDPQTLYELQSGLTGPE
                     GAAYPTHLAAYSSAQSSYTLDGGNELRVPLTWSEGGVNVTKTFVFRRGQYRIDLDYLV
                     DNGSRAAWPAREYAQILRNDPPTKRSYFNVESYAFHGPALWDGTKFRRLDTSDTQDSH
                     LSLDVHDGWVAALQHHFVSAIVPPRGAPWHFGLSAQGPEYLLSATGPEQTIAPGATGE
                     FDVALFAGPKLQGQLDATSPELGRVTDYGRLWFLARPLFVALSWVHKLTGNWGVAIIA
                     VTFLLKLLFYPLSEASGRSMAKMKTLQPRIKNLQETYADDREKLGRAMMELYKREKIN
                     PVAGCLPIIIQIPVFLAFYWVLLESVEMRQAPFAFWIHDLSSRDPYFILPLIMAGAMF
                     VQYKLNPAPPDPVQARVFMIMPLVMSATFAFFPAGLVLYWVTNTVLSIAQQWNINRRI
                     GAQPAKKN"
     gene            complement(3495..3737)
                     /gene="yidD"
                     /locus_tag="VI195_07630"
     CDS             complement(3495..3737)
                     /gene="yidD"
                     /locus_tag="VI195_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY6824296.1"
                     /translation="MRRFLAILIRLYQWTVSPLIGPACRYYPSCSQYALEAVLRFGVL
                     RGSGLARARRARCHPWHAGGFDPVPAVGRTHHTHEH"
     gene            complement(3722..4099)
                     /gene="rnpA"
                     /locus_tag="VI195_07635"
     CDS             complement(3722..4099)
                     /gene="rnpA"
                     /locus_tag="VI195_07635"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY6824297.1"
                     /translation="MIASAKSGRLTLPARLRLRRKGDFDDAYARGRRLSDGFFSVTVK
                     VNDAGAPRLGLAVAVKVAGSAVARNRLRRIIRESFRLHQRELPTVDLVVSARPAARTA
                     TAAALRTSLAMLWKRIGEQCAAS"
     gene            complement(4155..4289)
                     /gene="rpmH"
                     /locus_tag="VI195_07640"
     CDS             complement(4155..4289)
                     /gene="rpmH"
                     /locus_tag="VI195_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY6824298.1"
                     /translation="MKRTYQPSKVRRKRTHGFRARMATKNGRKVLARRRAKGRRKLIP
                     "
     gene            4497..>5179
                     /locus_tag="VI195_07645"
     CDS             4497..>5179
                     /locus_tag="VI195_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY6824299.1"
                     /translation="MGALEAELPEQQFNTWVRPLQAMEGNGALKLLAPNRFVVDWVNA
                     NLLPRIGELLRDESTGDAPIVTVEVGSRSVPLRGAPPVVIGAVPRRAEGLVVGARLNA
                     DFTFASFVEGKSNQLAKAAALQVAENPGRAYNPLFLYGGVGLGKTHLMHSIAHLIKDN
                     DAGARIAYVHSERFVGDMVRALQHNSMNEFKTAYRSLDALLIDDIQFFAGKERSQEEF
                     FHTFNELLEG"
BASE COUNT          874 a         1705 c         1754 g          846 t
ORIGIN      
        1 ccgccgccgc cctgggccca ctggctgccg aagaacacga ggtacacccg cggcgcgccg
       61 ctcgtgacgc caacctgatc gacgccaccg ccataggcca aggtgtcgct cgtgggagcg
      121 ccaaaaggat gtgcgctctc ccaggtactc atcttgtctg cgcattcgcg cgtaggcatg
      181 acgccgtagc ggtactggtg cccggactgg ggcgagtacg gattctcgta gcgtgtcggc
      241 tcggtactgc acgccgccag agacaggccg agaagcagag ccgtgttgcg caccgttttc
      301 cctgagatgg acatggcgtt ctccttggtc atcgagctgt gttgtctgta acgctcaacc
      361 tcactttccg atacagaatc cggcgaaaat ccgcccgagc aggtcgtcgc tggtgaactc
      421 gcccgtgatc tcgttgagag cctgctgcgc caggcgcagt tcctcggcaa ccagctcgcc
      481 ggcccgccgc tcggtcagca gccgctcggc ctcctcggtg tgcgtgcgcg cgcgctcgag
      541 cgactcgaga tgacggcggc gcgcgctgat cgctccgcca tctacgctct gatagcccat
      601 ggaagtcttc aagtgcgacc gcagttcggc gagccccgcg ccggtgagcg ccgagagggc
      661 gaagcgtggc ggtccatcgg ggtcgttggc aaccgcaaac ccgggcgcga gatcgcactt
      721 gttgaagagg agcgtgagcg ggacgtctgc gggtaaccgc gcgcgttccc gccgtagcgc
      781 cccgccgtcg ggatctgtgg cggcatcgat cacgaagagc acccgatcgg cgtgctgcat
      841 ttccgcctgc gcgcgtcgca tgccctcctc ctcgacgagg tcgcctccct cacgcagacc
      901 cgcggtgtcg aggatgtgca gcggcatgcc atcgaggtga atgcgctcgc gcagcacatc
      961 gcgagtcgtg cccggaatgg atgtgacgat cgcggcatcg tagccggcga ggcgattgag
     1021 caggctcgac ttgccggcat tcgggggtcc ggcgatgacc accgtcatcc cctcgcgcag
     1081 cagcctgccg atccgcgcgc tctcgagcac accggcgaag tgctcgcgca ccgcctggaa
     1141 acgctcgccg agctcacgat cggccaggaa gtcgatctct tcctcgggaa agtcgatcgc
     1201 cgcctcgaca taggtgcgca gctcgatcac cgcctcggtg aggccgcgca ccatcgcgga
     1261 aaattctccg cggagcgagc gcatggcggc gcgcgcggcg gcgcgcgagc cggcatcgat
     1321 gagatcggcg atggcttcag cttgagccag atcgagcttg tcgttgagga acgcgcgctg
     1381 cgtgaactca ccgggcagcg cgcggcgcgc cccgagctcg agcacgcgct cgaccagcgc
     1441 ctccaccacg agcgggccgc cgtgaccgtg cagctcgagc acatgctcgc ccgtatacga
     1501 gtgcggcgcc gggaaaaaga gcgcgagacc cgcatcgatc gggcactgct gcgcatcgag
     1561 gaagcgcgcg aaggtcgcgt agcgcgccgc aggcacctcc cccagcagga tggcggcaag
     1621 ctcgggggtc ttcggtcccg agacccgcac gatgccgacg ccaccgcgtc ccggtggcgt
     1681 tgccgcggcc acgatggtct gcgtgcgcgt catctgcggc tgcgcatcgt cttgggcccg
     1741 gaagcaaggc taccgcgggc gagcgctgca aaccaagcgt tcagttcttc ttcgccggct
     1801 gcgcaccgat gcgccggttg atgttccact gctgtgcgat cgagaggacc gtgttggtca
     1861 cccagtagag caccagcccc gcaggaaaga acgcgaaggt ggccgacatc accagcggca
     1921 tgatcatgaa cacccgtgcc tgcaccggat cgggcggggc aggattgagc ttgtactgca
     1981 cgaacatcgc accggccatg atgagcggca ggatgaaata gggatcgcgc gaggacagat
     2041 cgtggatcca gaaggcgaac ggcgcctggc gcatctccac gctctcgagc agcacccagt
     2101 agaaggcgag gaacaccgga atctggatga tgatgggcag gcagcctgcg accggattga
     2161 tcttctcgcg cttgtagagc tccatcatgg cgcggccgag cttctcgcga tcgtcggcgt
     2221 aggtctcctg caggttcttg atgcgcggct gcagcgtctt catcttcgcc atcgagcggc
     2281 cgctcgcctc cgagagcggg tagaacagca gcttcaggag gaaggtcacc gcgatgatcg
     2341 cgacgcccca gttgccggtc agcttgtgca cccagctgag cgcaacgaaa agcggccgcg
     2401 ccaggaacca cagccggccg tagtcggtga cgcgcccgag ctccggtgag gtcgcatcga
     2461 gctggccctg gagcttcggt cccgcgaaga gcgctacatc gaattccccg gtggccccgg
     2521 gagcaatggt ctgctcgggg ccggtggcgc tcagcaggta ctccggcccc tgcgcactga
     2581 gcccgaagtg ccacggtgca ccgcgcggcg gcacgatggc gctcacgaag tggtgctgca
     2641 gcgccgccac ccagccatcg tgcacgtcga gcgagaggtg gctgtcctgt gtgtcgctcg
     2701 tgtcgagccg gcggaacttg gtgccgtccc agagcgccgg gccgtggaag gcgtagctct
     2761 cgacgttgaa gtaggagcgc ttggtcggcg gatcgttgcg cagaatctgc gcgtactcgc
     2821 gcgccggcca ggcggcgcgg ctgccgttgt ccaccaggta atcgagatcg atgcggtact
     2881 gcccgcgccg gaacacgaac gtcttggtga cgttgacgcc gccctcgctc caggtgagcg
     2941 gcacgcgcag ctcattgccg ccatcgagcg tgtagctcga ttgcgcgctg ctgtaggctg
     3001 cgagatgcgt cgggtatgca gcgccctccg gcccggtgag gcccgactgc agctcataga
     3061 gtgtttgcgg atcgtcctga ttctgcagcc gcaccggcgc cgcctcgcct ttcactctcg
     3121 gataggcgag cagatccacg cgcgcgagcg taccgccgcg cgtgctgata tcgacatcga
     3181 ggacatccgt gcgcacatgc acgaccggcg cggccgccgc gggctcgggg gtggccgccg
     3241 cgcccgccac acccgtcgca cccgccgcac tttgcggcac gggcgcggag ctcggtgcgc
     3301 tcgtgctagg agagaccgcc tcggggacgc ggttgccaag agctccgctt cctggcgcgc
     3361 tcgatggcgc ggcggctgca ggagccgatg ccgggacggg tggtggcgcg taatcgtgca
     3421 cccacagctg gtagtcatag agcagcacca tggcgagcgc tgcccagaga tagacgcgca
     3481 gattcccgag ccggttagtg ttcatgggtg tggtgcgtgc ggcctaccgc cggaacggga
     3541 tcgaagccac ccgcgtgcca cggatggcag cgtgcgcgcc gcgcgcgcgc gagcccactc
     3601 ccgcggagca cgccaaaacg cagcaccgcc tcgagcgcat actgcgagca gctcgggtag
     3661 tagcggcatg cgggcccgat aagtggactc acggtccact ggtagagacg gatcaggatc
     3721 gctaggaagc ggcgcactgc tcacctattc tcttccacag catggcgagg ctcgtgcgca
     3781 gcgccgcggc ggtggcggtc ctcgcggcgg gccgcgcact caccacgaga tcgacagtcg
     3841 gcagctcgcg ctgatggaga cggaaggatt cgcggatgat gcgccgcaga cgattgcgcg
     3901 ccaccgcgct gcccgcgacc ttgacagcca cggccaatcc caggcgcggt gcgccggcat
     3961 cattcacttt cacggtcact gagaagaatc catcactcag gcgccgcccc cgcgcgtacg
     4021 cgtcatcgaa atcacccttg cgccgcaggc gaagccgcgc cggcagagtc agccgcccgg
     4081 acttcgcact ggcaatcaag atcggaagga ggggcagcgg ttccgctgct gcagcgcgtg
     4141 ccaccccgaa gctttcaggg tatcagcttg cgccgtccct tggcgcggcg acgcgcgagc
     4201 accttgcggc cgttcttggt cgccatgcgt gcacggaacc catgggtgcg cttgcggcgc
     4261 actttgctcg gctggtaggt ccgcttcatg gcgtcggctc cgtggaacga tggcggctgt
     4321 caaaaaaggg cggcaaggct aagccgttga gcgccaaggt gtcaatacgg gcgcgctgtg
     4381 gataactctt cagcgcggag tgcgatcggt atagacttcg cgaccttttc ctcaacgttc
     4441 acaagaaaga ctcgcggagg ccacgcgcgg tggaagcgtc gctttggacc cgctgcatgg
     4501 gcgcgttgga agcggagctt ccggagcagc aattcaatac ctgggtgcgg ccgctgcagg
     4561 ccatggaagg caatggggcg ctgaagctcc ttgcgccgaa ccgtttcgtc gtcgattggg
     4621 tgaatgccaa tctgctgccg cgcatcggtg agctgctgcg cgatgagagc accggcgatg
     4681 cgcccatcgt gacggtggaa gtcggctcgc gcagcgtgcc gctgcgtggc gcaccaccgg
     4741 tggtgatcgg cgccgtcccg cggcgtgcgg aggggctggt cgtcggcgcg cggctcaatg
     4801 ccgatttcac cttcgcgagc ttcgtcgagg gcaagagtaa ccagctcgcc aaggctgcgg
     4861 cgctccaggt ggccgaaaat cccggccgtg cgtataaccc gttgttcctc tacggtggcg
     4921 tcggcctcgg caagacgcat ctcatgcact cgatcgcgca cctgatcaag gacaacgatg
     4981 caggcgcgcg catcgcctac gtgcactccg agcgcttcgt cggcgacatg gtgcgtgcgc
     5041 tccagcacaa ctcgatgaac gagttcaaga cggcctaccg ctctctcgat gcactgctga
     5101 tcgatgacat tcagttcttc gcgggcaagg agcgctcgca ggaggaattc ttccacacct
     5161 tcaacgagct gctggaggg
//
LOCUS       DATDMP010000436         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1403833, whole genome shotgun sequence.
ACCESSION   DATDMP010000436 DATDMP010000000
VERSION     DATDMP010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403833"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VI195_07650"
     CDS             complement(<1..1259)
                     /locus_tag="VI195_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824300.1"
                     /translation="MPTVGAPGTAAPRLRFALLALAVALTLLIALLAAYELAAARVPQ
                     HRAALEELIRRQTGLEVRFAGLLVRWGWYGPEALFEDVELGEPQGRGLLLHARRLIVS
                     LDAWRMVRSGHLEARRITLEDPIIDLAGDAQARSGGAVTRSPAQARGMGARILARWRG
                     GQINISGGTLRTVLPGGTDAVTLGISRAELRRVDADWSAEAQILLPHVLGKSMHLALQ
                     MRARPDLEDISGATVRVDGRQLELAAWAGLAGTDAWSELPRSGSADLDLQAAFVHGRL
                     RAASGHIAAESLEWRASSDSDPLTLERVRAKWQLRRRGAEWHVTVDALELESPATNSS
                     AAPASVVVDFAPDGSHARGRALHAPLAPLLSFVRWYVPRLPPSGLVFRGEARELSFDW
                     NAHRPPGTRLAASAQLESLALADDSGAV"
     gene            complement(1306..>2207)
                     /locus_tag="VI195_07655"
     CDS             complement(1306..>2207)
                     /locus_tag="VI195_07655"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY6824301.1"
                     /translation="KLWEHVRTRVAQVPPGSVVHEDLPLTLRVLRDELARGVARVLVD
                     SPREYERMREFAAAFMPQGAPAIELYCAPRPIFDLSGIEEEIAKALDRKVSLKSGGHL
                     VIDQTEAMTTIDVNTGAYVGHRNLEETIFRTNLEAAVSIARQLRLRNLGGIIIIDFID
                     MRDEPHRRAVLSALERALAGDRAQTHIVSLSPLGLVEMTRKRTRESLEHLLCKPCPSC
                     EGRGFVRTPETVCNEIFREIVRQSRQFASRELLILAHQDVVDRLLDEESATLAELEAQ
                     VARPIRLQVEALYGVDQYDVVLV"
BASE COUNT          364 a          760 c          749 g          334 t
ORIGIN      
        1 accgcaccgg aatcatctgc aagtgccagg ctctcgagct gtgccgatgc ggcgagccgc
       61 gtccccgggg ggcgatgcgc attccaatcg aagctcagct cgcgcgcctc gccccgaaac
      121 acgagcccgc tgggcggcaa ccggggaaca taccaacgca cgaacgagag cagcggagcg
      181 agcggtgcat gcagcgcgcg gccgcgagcg tgactaccgt ccggcgcgaa gtccacgacg
      241 accgaagccg gcgcggccga ggagttggtt gcagggcttt cgagctcgag cgcatcgacg
      301 gtgacatgcc actccgcccc acgccgcctg agctgccatt tggcgcgcac gcgctcgagc
      361 gtgagaggat ccgagtcgct gctggctcgc cattcgagcg actccgcggc gatatgtccg
      421 gaggcagcgc gcaggcgtcc atgcacgaaa gcggcctgaa ggtccaggtc ggcacttccc
      481 gagcggggca gttcgctcca tgcgtcagta ccggcgagtc cagcccacgc ggcgagctcg
      541 agctgacggc catcgaccct cacggtggcg cccgagatat cctcgagatc gggtctggcg
      601 cgcatctgca gcgccagatg catgctcttg ccgagcacgt gcggcagcag gatttgcgct
      661 tcggcactcc agtccgcatc gacccgccgc agctctgcgc gcgagattcc aagagttacc
      721 gcatcggtgc cgcccggcag gaccgtgcgc agcgtgccgc cggagatgtt gatctgtcct
      781 ccacgccagc gcgcgaggat gcgggccccc atgccgcgcg cttgtgcggg gctgcgcgtc
      841 accgcgccgc cgctgcgggc ctgcgcgtcg ccggcgagat cgatgatggg atcctcgagc
      901 gtaatgcgcc gggcttcgag gtggccgctg cgcaccatgc gccaggcgtc cagactgacg
      961 atcaacctgc gcgcatgcag cagcaagccc ctgccctgtg gctcgccgag ctcgacgtcc
     1021 tcgaatagcg cttccggacc gtaccatccc caacgtacca gcaggccggc gaagcgcacc
     1081 tcgaggccgg tctggcgacg aatgagctcc tcgagcgctg cgcggtgctg cggcacacgt
     1141 gccgcagcca gctcgtacgc cgccagcagc gcgatgagca gcgtgagtgc cacggccagt
     1201 gcgagcagag cgaagcgcag ccgcggggcg gccgtgcccg gggcgccgac ggtcggcacg
     1261 gcgactgcag gctcagctga cgtaccagcg aacacttgga agacctcaga cgagtacgac
     1321 gtcgtattga tcaacgccgt agagcgcctc gacctgcagg cggatcggcc gcgctacctg
     1381 ggcttccagc tctgcgagcg tggctgattc ctcatcgagc aggcgatcca cgacgtcctg
     1441 atgcgccagt atcagcagct cgcggctggc aaactgccgg ctctgtcgca caatctcgcg
     1501 aaagatctcg ttgcacacgg tttccggagt gcgcacgaag ccgcgcccct cgcagcttgg
     1561 acagggcttg cacagcagat gttccaggct ctcgcgcgtg cgcttgcggg tcatctcgac
     1621 caggccgagc ggcgagagac tgacgatgtg cgtctgtgcc cggtcgccgg cgagcgcgcg
     1681 ctcgagcgcg ctcagcacgg cgcgccgatg cggctcgtcg cgcatgtcga tgaagtcgat
     1741 gatgatgatg ccaccgaggt tgcgcaaccg cagttgccgc gcaatgctca ccgccgcctc
     1801 gagattggtg cgaaagatgg tctcctcgag gttgcggtgg ccgacgtagg ctccggtgtt
     1861 gacatcgatg gtggtcatcg cctcggtctg atcgatcacc agatgtccgc cggatttcag
     1921 cgacaccttg cgatcgagtg ccttggcgat ctcctcctcg atgccgctca gatcgaagat
     1981 cggccgcggg gcgcagtaca gctcgatcgc cggcgcaccc tgcggcatga aggcggcggc
     2041 gaactcgcgc atgcgctcgt actcgcgcgg cgaatcgacg agtacgcgcg cgacgccgcg
     2101 cgcgagctca tcgcgcagca cgcgcagcgt gagcggcagg tcctcgtgca ccacgctgcc
     2161 gggcggcacc tgggcgacgc gcgtgcgcac gtgctcccac agcttgg
//
LOCUS       DATDMP010000437         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1403871, whole genome shotgun sequence.
ACCESSION   DATDMP010000437 DATDMP010000000
VERSION     DATDMP010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403871"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VI195_07660"
     CDS             complement(<1..1678)
                     /locus_tag="VI195_07660"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY6824302.1"
                     /translation="MPQTKFRFFFIALLLVLAAAGAISLIALRSLPRVSGEVIVRGIE
                     RPVEVLRDTDGIPHIFAQSEADAHFALGFVHAQDRLWQMEMNRRIAAGRLAEVLGPAA
                     LDTDRFLRTLGIRRVAEANIGALDADSRRLLAAYTAGVNAFLATKPVVPPEFWLLGVT
                     PEPWSEVDSAAWVKMLAWDLGGNWRSELLRMQLAHSLPTAAIQQFLPPYPGDKPVELP
                     DLAALYGKPAGPLVPGVSDAPAGGSNSWVVSGAHTKSGKPLLANDPHLGLTAPSIWYF
                     AHLHAPGLNAIGATLPGVPGIAIGRNERIAWAATNTGPDVQDLYLERLDSAARYLAPN
                     GWHEFETRREIIHVKGAADVELMVRLSRHGPVISDVLASAAAAAPRGHVLALAWTAFS
                     DDDTSLAAILGMPRARNWPEFLAATRALLAPQQNLSYADIDGNIGFIAPGRIPVRKPE
                     NVLRGLAPAPGWDARYDWQGYLPFEDLPRAFNPSSGRIVTANQKIVPPGYRHQITSEW
                     QPPYRAQRIAELLDLSEQHDRASFARIQMDVVSLPAGELLPRMLRTEPKSA"
     gene            1730..>2288
                     /locus_tag="VI195_07665"
     CDS             1730..>2288
                     /locus_tag="VI195_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY6824303.1"
                     /translation="MLYSLYEAQHLALEPWRYLAEWSRGWFGHPFSPFAHLPISRRVA
                     ASSDLFLRVTERYEKPKWNLPIEPEVVHAKPFGHLLHFPQSTPKRHKVLIVAPLSGHH
                     ATLLRDTVRALLAEHDVWITDWVNARLVPLSQGPFHLADYVDYVREWIGFLSPGVHVV
                     SVCQPTVPVLAAVSLMAVSREAQPLT"
BASE COUNT          388 a          754 c          766 g          380 t
ORIGIN      
        1 cggcgctctt tggctcggtg cgcagcatgc gcggcagcag ctcgcccgcc ggcagcgaga
       61 ctacgtccat ctgaatgcgg gcgaacgaag cgcgatcatg ctgttccgac aggtcaagga
      121 gctcggcgat ccgctgcgcg cgataaggcg gctgccattc gctggtgatc tggtggcgat
      181 agcccggcgg cacgatcttc tggttggcgg tgacgatgcg gccgctcgat gggttaaagg
      241 cgcgcggcag atcctcgaac ggcagatagc cctgccagtc gtagcgcgca tcccaacccg
      301 gcgcgggcgc gagcccgcgc agcacgttct ccggcttgcg caccgggatg cggccgggcg
      361 caatgaagcc aatattgccg tcgatgtccg catagctgag gttttgctga ggggcgagca
      421 gcgcgcgcgt cgctgccaga aactcaggcc agttgcgtgc gcgtggcatg ccaaggatgg
      481 cggcgaggga tgtgtcgtcg tcggagaaag ccgtccaggc gagcgcaagc acgtggccgc
      541 gcggggccgc cgcggctgcg ctcgccaaga catccgagat caccgggccg tgccgggaca
      601 aacgcaccat cagctcgaca tcggcagcgc ctttcacgtg gatgatctcg cgacgggtct
      661 cgaactcgtg ccagccgttc ggtgccaggt agcgcgccgc tgaatcgagg cgctccaggt
      721 acaggtcctg aacgtccggt ccggtgttcg ttgccgccca ggcgatgcgc tcgttgcgcc
      781 cgatcgcgat gccgggcacc ccgggcagcg tggcgccgat cgcgttcagt ccgggcgcat
      841 gcaggtgcgc gaagtaccag atgctggggg ccgtgaggcc aaggtgcggg tcgttggcca
      901 ggagcggttt gccgctcttg gtgtgcgcgc cggacaccac ccagctattg gagccccccg
      961 ccggtgcgtc gctcacgcct ggaacgagcg ggcccgcggg tttgccgtac agggcggcaa
     1021 gatccggcag ttcgaccggc ttgtcgcccg gataaggcgg caggaattgc tggatggcgg
     1081 ccgtcggcag gctgtgcgcg agttgcatcc gcaggagctc gcttcgccag ttaccgccca
     1141 agtcccaggc cagcatcttt acccacgcgg ccgaatcgac ttccgaccac ggctcgggcg
     1201 taactcccag cagccagaat tccggcggca ccaccggctt tgtggcgagg aaggcattga
     1261 ctccggcggt gtacgcagcg agcaagcggc ggctgtcggc atcaagagcg ccaatgttcg
     1321 cctcggcgac tcggcggatg ccgagggtac gcaggaagcg atcggtatcc agcgcggccg
     1381 gtccaagcac ctcggcgagc cggccggcgg cgatccggcg gttcatttcc atctgccaga
     1441 gccggtcctg cgcatggacg aaaccaagcg cgaagtgagc gtctgcttcg gattgagcaa
     1501 agatgtgcgg gataccgtcg gtatcccgca acacctcgac cggtctttcg atgcctctaa
     1561 caatcacctc gccgctaacc cgcggcagcg agcgcagtgc aatcaacgag atggcgcccg
     1621 cggccgccag gaccagcaat aacgctatga aaaagaaccg gaacttggtc tgcggcaccg
     1681 cgtcaactct gttataagcc gatagttacg acaacgttac cgcgactgga tgctctacag
     1741 cctgtacgaa gcacagcacc tcgccctgga gccttggcgc tacctggcgg agtggtcgcg
     1801 cggatggttc ggccatccct tcagtccatt cgcccacctg ccgatctccc ggcgtgtcgc
     1861 ggcttcgagc gacctcttcc tgcgggtcac ggagcgctac gagaagccga aatggaacct
     1921 gccgatcgaa cccgaggtcg tgcacgcgaa gccgttcggc cacctgctgc atttcccgca
     1981 gtcgacgccg aagcgccaca aggtcctgat cgtcgccccg ctctccgggc accacgcgac
     2041 gctgctgcgc gatacggtgc gcgcgctcct cgccgagcac gacgtctgga tcaccgactg
     2101 ggtgaacgcg cggctggtac cgctttcgca ggggccgttc catctggccg actacgtcga
     2161 ctacgtgcgc gaatggatcg ggttcctgtc ccccggagtg cacgtcgtct cggtatgcca
     2221 gccgaccgtg ccggtgctcg ccgcagtttc gctcatggcg gtaagtcggg aagcgcagcc
     2281 actcacca
//
LOCUS       DATDMP010000438         2454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1292932, whole genome shotgun sequence.
ACCESSION   DATDMP010000438 DATDMP010000000
VERSION     DATDMP010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292932"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VI195_07670"
     CDS             complement(<1..1038)
                     /locus_tag="VI195_07670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824304.1"
                     /translation="MSGRAVAVLALGGFASAAAQRAADPLLPLLAQAFGTTAGGASAV
                     ITAFAVAYGGLQLVNGPIGDRIGKYRMVFWVTAISAAGNLACALAPTLPLLVAARFAS
                     GATVGAIVPLAIAWIGDAVPYERRQTVLARFLIGSMLGGAVTTTLSGVLGEQFGWQAI
                     FYVLAGLYVIAAGLLYAELRSNPATQSRAAAREPMTLAFARMLALTRDRWVRVVLSTV
                     FIEGALSQGGLAFAAFHGHQALGLSVAASGMLVASFALGGLLYSVVAGRLVPRLGERG
                     LVIGGGLLLCVGLPGFAAAPGPFWAIPCLVAQGMGLFMLHNTLQVHATQMAPNSRGAA
                     LALFAFSLFTGQ"
     gene            complement(1046..>2454)
                     /locus_tag="VI195_07675"
     CDS             complement(1046..>2454)
                     /locus_tag="VI195_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6824305.1"
                     /translation="VTVSGRFSYKDLFEKAKRAAGAMQALGIGKGDRVAILLPNGEDW
                     LACFYGAALIGAVTVPVNTRFKSAEIAFCLKQSSAMALFYVGRFLNNDYAAMVEEIGF
                     DLAVDLSSRELKGEFRPVPVEPEDLLLIQFTSGTTAYPKGAMLTHDSMLRNAWAAGTR
                     IGVRADDRYFNCRPFFHVGGSTLSALMSLVSGCCLVTLPSFEAGAALAMMERERCTLT
                     SGNDTIFQMLMGHEDFPKRKLDLRGGWAAAGPQTMRQIIDRMGMKAVCAAYGLSEASP
                     NVVMSDARDAEALRVDGLALPLPGLEIRISGEKEIQVRGWSLMRGYVNDPEATAKAFT
                     QDGWLRTGDLGELTQDGRLRMSGRMKDVFRVGGENVAPAEVEEVLLAHPAVETAQVVG
                     VPDARLGEVPCAYVTLKSGFTISEEELKNWAREKAANFRVPRYLRIVSDFESIGMTAS
                     GKVQKQKLRAHALKEFKL"
BASE COUNT          423 a          865 c          817 g          349 t
ORIGIN      
        1 ttgcccggta aacaggctga acgcgaaaag cgcgagcgcc gccccgcgcg agttcggcgc
       61 catctgcgtc gcatgcacct gcagcgtgtt gtgcaacatg aacaacccca tgccctgcgc
      121 gaccaggcag ggaatcgccc agaacggccc aggggcggcc gcgaagccgg gcagtcccac
      181 gcacagcagc agcccgccgc cgattaccag gccgcgctcg cccagccgcg gcaccagtcg
      241 cccggcgacg acagagtaga gcagcccgcc gagcgcgaag ctggcgacca gcatgccgct
      301 cgccgccacc gagagcccga gcgcctggtg gccgtggaac gcggcgaacg ccaggccgcc
      361 ctgcgaaagc gcgccttcga tgaacacagt ggagagcacg acgcgcaccc agcggtcgcg
      421 cgtgagcgcc agcatgcgcg cgaaggcgag cgtcatcggc tcgcgcgctg cggcgcggga
      481 ctgcgtcgcc gggttcgagc gcagctcggc gtacagcagc cccgcagcga tgacgtagag
      541 accggcgagc acgtagaaga tcgcctgcca gccaaattgc tcgccgagca cgcccgacag
      601 cgtcgtggtc acggcgccgc ccagcatgct gccgatgagg aagcgcgcga gcacggtctg
      661 gcggcgctcg tagggcaccg cgtcgccaat ccaggcgatg gcgagcggca cgatcgcgcc
      721 caccgtggcg ccgctggcga agcgcgcggc gacgagcagc ggcagcgtgg gcgcgagcgc
      781 gcaggcgagg tttcccgccg cggagatcgc cgtcacccag aacaccatgc ggtacttgcc
      841 gatgcggtcg ccgatcgggc cgttgaccag ctgcaggccg ccgtaggcga cggcaaatgc
      901 cgtgatcacc gccgaagcgc cgcccgccgt ggtgccgaac gcctgggcga gcagcggcag
      961 cagcgggtcc gcggcgcgct gcgcggcggc gctggcgaat ccgccgagcg cgaggacggc
     1021 aacggcgcgg ccgctcatct tcccgtcaaa gcttgaattc cttcagcgcg tgcgcgcgca
     1081 gcttctgctt ctgcaccttg ccgctcgcgg tcatgccgat ggactcgaag tcggaaacga
     1141 tgcgcaggta gcgcggcacg cgaaagttgg ctgctttttc tcttgcccag tttttcagtt
     1201 cttcttccga aatggtgaaa ccggatttca aggtcacgta ggcgcaaggc acttcgccaa
     1261 gacgcgcatc cggcacgccg accacctggg ccgtctccac agccggatgc gcgagcagca
     1321 cctcctccac ttccgcgggc gcgacgttct cgccgccgac gcggaacacg tccttcatgc
     1381 ggcccgacat gcgcagacgg ccgtcttgcg tgagctcgcc gaggtcgccc gtgcgcagcc
     1441 agccgtcctg cgtgaacgcc ttcgcggtcg cctccggatc gttgacgtag ccccgcatca
     1501 ggctccagcc gcgaacctgg atctcctttt ccccggagat ccgtatctcg agcccgggca
     1561 ggggcagcgc aaggccgtcc acgcgaagcg cctccgcatc gcgcgcgtcg ctcatcacca
     1621 cgttgggcga agcctcggac aagccgtagg cggcgcacac cgccttcatg cccatgcggt
     1681 cgatgatctg gcgcatggtt tgcggcccag ccgcggccca gccgccgcgc aggtcgagct
     1741 tgcgcttcgg gaagtcttcg tggcccatga gcatctggaa gatcgtgtcg ttgcccgagg
     1801 tgagcgtgca gcgctcgcgc tccatcatcg ccagcgccgc gcccgcctcg aagctgggca
     1861 gcgtgaccag gcagcagccg gacacgagcg acatcagggc cgaaagcgtc gagccgccga
     1921 catggaagaa cggccggcag ttgaagtagc gatcgtcggc gcgcacgccg atgcgcgtgc
     1981 ccgcggccca ggcgttgcgc agcatgctgt cgtgcgtgag catcgcgccc ttcgggtacg
     2041 ccgtcgtgcc ggacgtgaac tggatgagca ggaggtcctc gggttcgacc ggcacgggcc
     2101 tgaattcgcc cttcagctcc ctgctcgaaa ggtcgacggc aagatcgaaa ccgatttcct
     2161 cgaccatcgc ggcatagtcg ttgttcagga agcggcccac gtagaacaac gccatcgcgc
     2221 tcgattgctt caggcagaag gcgatctcgg ccgacttgaa gcgcgtgttc accggcaccg
     2281 tcaccgcgcc gatgagcgcc gcgccgtaga agcaggccag ccagtcctcg ccgttgggca
     2341 gcaggatcgc cacgcggtcg cccttgccga tgcccagggc ctgcatcgca ccggcggccc
     2401 gcttcgcttt ttcaaataga tctttgtagg aaaaacggcc tgaaacagtg acca
//
LOCUS       DATDMP010000439         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1514037, whole genome shotgun sequence.
ACCESSION   DATDMP010000439 DATDMP010000000
VERSION     DATDMP010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514037"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VI195_07680"
     CDS             complement(<1..1117)
                     /locus_tag="VI195_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY6824306.1"
                     /translation="MYRSQRLALRYFTAAIVLFGLMTAAGLLAATYYIQPSLLYNVFD
                     FHMAKILHINTLVIWLLMGFIGAVYWFLPNELQREVVGVRMAEILFYVFCAAVGVVAV
                     VFLFVQYGGSNQFSMWFINQGRKYVEAPRWAALGIVVVVLTFVYNVVATAIRARRMTG
                     ILWVLMLDLVPLSVLYLDAFPQITNMSHDLYWWWWLVHMWVEATWEVLIGCLMAWSLM
                     ELLGTSRRIVETWLYIEVMLVLGTGILGLGHHYFWIGTPQYWLSIGGFFSALEPLPLL
                     GMVVHAVYDAGTHRMHTTNRPAFYWVLAEAFGNFIGAGVFGFMMTLPQINLFSHGTQW
                     TVSHGHFAFWGAYGCGVIAILYLVLQRTRGAQILESRA"
     gene            complement(1203..1742)
                     /locus_tag="VI195_07685"
     CDS             complement(1203..1742)
                     /locus_tag="VI195_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6824307.1"
                     /translation="MLVVLAFLTIDSLDAIHAGGRQVPNYDVINQRIGYAYDWSRGFD
                     VPVIGPAEPLFGKPYTLAQATAQIRLGKLVIQSRACMDCHTFFGNGAYYGPDLTKAWL
                     DPAWTQIWMPMTGASTRADAMVLFLMHPERYPTWTRRMPNLGLSEEEARSLVAYLKWM
                     ASVDANGFPENFGLIHSRD"
     gene            complement(1924..>2634)
                     /locus_tag="VI195_07690"
     CDS             complement(1924..>2634)
                     /locus_tag="VI195_07690"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEY6824308.1"
                     /translation="GGLLERTHTDIGVVEGIVGIHWWEATVTGIANHAGTTPMDQRHD
                     ALLAASELVLAVNAAATELPGRQVATVGRIRAEPGAPNVIPGKVVLSVEVRDLDAARI
                     DAVFERIRTRAAAIAEARGTSIEFRDPEANAVPALTDPRLQRIIDGAAHALGLKTMLM
                     PSGAGHDAQDIAAIAPIGMIFVPSAGGISHSPREYTAPADIAHGVNVLMRAVLAIDAG
                     ALDGNPVPAIRSGSTSKQ"
BASE COUNT          545 a          820 c          814 g          455 t
ORIGIN      
        1 aggcgcgact ctcgagaatc tgcgcgccgc gggtgcgctg cagcaccaga tagagaatcg
       61 caatcacgcc gcagccatat gcgccccaga aggcaaaatg cccatgcgag accgtccact
      121 gcgtgccgtg tgagaagaga ttgatctgcg gcagtgtcat catgaagcca aagacgcccg
      181 ccccgatgaa gttgccgaaa gcctccgcta gcacccagta gaaggccggg cggttggtgg
      241 tatgcatgcg gtgcgtgccg gcgtcatata cggcatgcac gaccatcccg aggagcggca
      301 agggctcgag ggccgaaaag aacccgccga tgctcaacca atactgcggc gtaccgatcc
      361 agaagtaatg gtggcccaag ccgagtattc ccgtacccag caccagcatc acctctatgt
      421 acagccacgt ttcgacgatg cggcgcgaag tgccgagcag ctccatgagg gaccaggcca
      481 tcaggcagcc gatcagcacc tcccacgtgg cttcgaccca catgtgcacc agccaccacc
      541 accagtaaag atcgtgcgac atgttggtga tctgcggaaa cgcatcgaga tagagcacac
      601 tcagcggtac gagatcgagc atcagcaccc agagaattcc ggtcatccgc cgggcgcgta
      661 tcgcagtagc gactacgttg tagacgaagg tcagcaccac gacaacaatg ccgagcgcgg
      721 cccagcgggg cgcttcgacg tacttgcgtc cctgattgat gaaccacatg ctgaattgat
      781 tgctgccgcc gtactgcacg aagagaaaca cgaccgcgac gaccccgacc gcggcgcaaa
      841 agacataaaa gaggatctcg gccatacgga cgcccacgac ctcgcgttgg agctcgttcg
      901 gcaggaacca gtacaccgca ccgataaagc ccatcagcag ccagatgacc agggtgttga
      961 tgtgcaggat cttcgccatg tggaagtcga acacgttgta aagcagcgat ggctgtatgt
     1021 aatacgtggc ggcgagcaga cccgcggccg tcatcaggcc gaacagtacg atcgcagcgg
     1081 tgaagtagcg cagggcaagc cgttgggagc gatacatggc gaactcctaa tgatgcgtcc
     1141 agcggtcgct gcttgccgaa agctggttgt tgcgcgctgt tggcgccatt tccttgcgaa
     1201 cctcagtcgc gactgtgaat gagaccgaaa ttctccggaa aaccgttggc gtcgaccgac
     1261 gccatccact tgaggtacgc aaccagtgac cgagcctcct cttcggaaag cccgagattc
     1321 ggcatgcgcc gcgtccaggt cggatagcgc tcgggatgca tcaagaacag aaccatggcg
     1381 tcggcgcgcg tgctggcgcc cgtcatcggc atccagatct gagtccacgc cggatcgagc
     1441 caggccttgg tgaggtcagg gccgtagtag gcgccgttgc cgaagaacgt atggcagtcc
     1501 atgcacgccc ggctctggat tacgagcttc cccaaacgta tctgcgcggt ggcttgcgcg
     1561 agagtatagg gcttaccgaa cagcggctct gccggtccga tcaccggtac atcgaatccg
     1621 cgggaccaat cgtaggcata gccgatgcgc tgattgatca cgtcgtagtt cggcacctgc
     1681 cgcccgcccg catggatggc atcgaggctg tcgatagtga gaaaggcgag gacgacgagc
     1741 atgacggccg tgctgcttat tgcgccggcg cgccagaatt tcgggtcttg aatatccacg
     1801 gttcacccgt tggtgagcac tgccgtcctt gaaccgaggt tcctcgtcaa gccgcgctgt
     1861 gcacgctagg tgaaaaccga agcgccggga atacgtacat taacgcatga ggtttttggt
     1921 gcattactgc ttcgaggtcg agccggaccg gatcgctggc accgggttgc catcgagcgc
     1981 gcctgcatcg atcgccagta ccgcccgcat cagcacgttc acaccgtgcg cgatgtctgc
     2041 gggcgcggtg tattcacggg gcgaatggct gatgccgccc gcgctcggta caaaaatcat
     2101 gccaatggga gcgatagcgg caatgtcctg cgcgtcgtgt cccgcgccgc ttggcatcag
     2161 catggtcttc agcccgaggg cgtgcgctgc gccgtcgatg atgcgttgca gacgcggatc
     2221 ggtcagtgcg ggcaccgcgt tcgcctcggg atcccgaaac tcgatgctgg tgccgcgcgc
     2281 ctccgcgatt gccgcggcgc gcgtccgtat gcgctcgaac acagcgtcaa tccgtgcggc
     2341 atcgagatcc cgcacctcga cgctgagtac caccttgccc ggaatcacgt tcggggcccc
     2401 cggctccgcg cggatccgac cgacggtcgc gacctggcgg ccgggaagct cggtcgccgc
     2461 ggcatttacc gccagcacca gctcggatgc cgcaaggagt gcatcgtgac gctggtccat
     2521 cggagtggtg ccggcgtggt tcgcgatgcc ggtcacggtc gcctcccacc agtggatgcc
     2581 gacgatgccc tcgacgactc ctatatcggt atgcgtacgc tcgagcagac cgcc
//
LOCUS       DATDMP010000440         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1404509, whole genome shotgun sequence.
ACCESSION   DATDMP010000440 DATDMP010000000
VERSION     DATDMP010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404509"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..787
                     /gene="rpiA"
                     /locus_tag="VI195_07695"
     CDS             77..787
                     /gene="rpiA"
                     /locus_tag="VI195_07695"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY6824309.1"
                     /translation="MAGTVVSGTRKAAGGRTMTADTDAKKRRAALAALELIPPDTIIG
                     IGTGSTVNFLIEALATRPGLVRTAVSSSTESSTRLRAVGVALADLNELTELALYIDGA
                     DEATPARHLTKGGGGALTREKIIAAAARRFVCIVDDGKMVAALGRFPLPVEVIPMART
                     LVARILSARGARPVWREGFITDNGNHILDVHGLVIKDAPALERELNQIAGVVTVGLFA
                     VRPADVLLIGTAQGVDTG"
     gene            960..1553
                     /locus_tag="VI195_07700"
     CDS             960..1553
                     /locus_tag="VI195_07700"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY6824310.1"
                     /translation="MATTQSTIVLPPLPYALDALEPYMSRETLEYHYGKHHKGYVEKL
                     NGLIRGSDFSDASLEELVAESSGDIFNNAAQVWNHTFFWNCLSPRRQSPSKAVTQALD
                     KLGGFEAFQKQFDKAASGLFGSGWTWLVKDARDGALKIMTTADANTPVRDGHVALLTC
                     DVWEHAYYIDHRNDRAAYLRNFWPLVNWEFVVSNLAT"
     gene            complement(1731..1804)
                     /locus_tag="VI195_07705"
     tRNA            complement(1731..1804)
                     /locus_tag="VI195_07705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1770..1772),aa:Gln,seq:ctg)
     gene            complement(1841..2239)
                     /gene="rpsI"
                     /locus_tag="VI195_07710"
     CDS             complement(1841..2239)
                     /gene="rpsI"
                     /locus_tag="VI195_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY6824311.1"
                     /translation="MAATQQNHYGTGRRKTATARVYLKPGSGRITVNERSLDEFFGRE
                     TGRMIVRQPLEAVKLVYKFDIAVTVGGGGITGQAGAIRHGITRALIEYDESLRKPLRD
                     AGFVTRDAREVERKKVGRRKARRGTQYSKR"
     gene            complement(2251..>2532)
                     /gene="rplM"
                     /locus_tag="VI195_07715"
     CDS             complement(2251..>2532)
                     /gene="rplM"
                     /locus_tag="VI195_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY6824312.1"
                     /translation="GDHIVVVNAQKIRVTGRKLSDKIYYHHTGHIGGIKSIALEKLLN
                     EHPERAIEFAVKGMLPKNPLGRRMFRKLHVFAGSEHPHQAQQPKPLKLQ"
BASE COUNT          460 a          805 c          807 g          460 t
ORIGIN      
        1 aagaagaaat aggccttaac tcggcggcca cattgtggcc gccgagtcga attcgaagag
       61 accgtgcccg ctcaccatgg cgggcacagt cgtttctggg actcgcaaag cggccggtgg
      121 gcgcacgatg acggcagata cggatgcaaa gaagcggcgc gcggcactcg cagcgctcga
      181 attgatcccg ccggacacga tcatcggcat cggcaccggt tccaccgtca acttcctcat
      241 tgaagcactc gccacgcggc cgggcctggt gcgcacggcg gtatcgagct cgacagagag
      301 cagcacacgg ctacgcgctg tgggggtggc gctcgcagat ctgaacgagc tcacggagct
      361 cgccctctac atcgatggcg ccgacgaagc gaccccggcg cggcatctca ccaagggcgg
      421 cggtggcgca ctcacgcgcg aaaagatcat cgccgcagcg gcgcgacgct tcgtatgcat
      481 cgtcgacgac ggcaagatgg tcgccgcact cgggcgtttt ccgctgccgg tcgaggtgat
      541 tcccatggcc cgtacgctgg tggcccgcat cctcagcgcg cgcggcgcgc ggcccgtgtg
      601 gcgcgagggc ttcatcaccg ataacggcaa ccacatcctc gatgtccatg ggctggtcat
      661 aaaagacgcg cccgctctcg agcgtgagct caaccagatc gccggagtag tcactgtggg
      721 cttgttcgcg gtgcgcccgg cggatgtgct gctgatcggt accgcgcagg gagtcgatac
      781 gggctgagtg ccccattttg gcgctcggac ctgcggaggc ctccactggg ccgcccgccg
      841 tgaaggaccg gctgctcgct gcggcacttc ggcgctcgct cgtggtccga taggtgacgc
      901 tgcgcgtcgc agatacggag cctgcggcgt taccacacca cccatggagg acccgaagca
      961 tggcgaccac ccaatcaacg atcgtgctgc caccgctacc ttacgcgctg gacgcgctcg
     1021 agccgtacat gtcgcgtgag acactcgaat atcactatgg caagcaccac aaggggtatg
     1081 tggagaagct caacggtctc atccgcggca gcgatttctc ggacgcttcg ctcgaggagc
     1141 tggtcgcgga gtcctccggc gatattttca acaacgccgc gcaggtgtgg aatcacactt
     1201 tcttctggaa ctgtctgtca ccgcggcggc agagcccgag caaggccgtc acccaggcgc
     1261 tcgacaaact cggtggcttt gaagcctttc agaagcagtt cgacaaggcg gcgagcggcc
     1321 tgttcggctc cggctggaca tggctggtga aggatgcgcg cgacggcgcc ctgaagatca
     1381 tgacgactgc ggatgccaac acaccggtac gcgacgggca cgtggcactg ctcacttgcg
     1441 atgtgtggga gcatgcctac tacatcgatc accgcaacga ccgcgcggcg tacctgagga
     1501 acttctggcc gctggtcaac tgggagttcg tggtgtcgaa cctggctacc tgaaaaaagc
     1561 gctgaatcag gcgggccagc cgcgtgccgc gcgcagttca tgcaggtttt ggcaatagtt
     1621 caccgaacgg ttaactattc ctcaagacac tatcaggcgg gcggctcgcc caaaacggcc
     1681 ggaagatgaa gagaagaagg cccgtgaaac gccacgggcc ttcttgctat tggctggggg
     1741 actaggattc gaacctaggt aaacggagtc agagtccgtt gtcctaccgc tagacgatcc
     1801 cccaaagaac cacccacggc ctcggcaccc gacccctatt ttagcgcttc gagtactgcg
     1861 taccgcggcg cgctttgcgg cggccgactt tcttgcgctc gacctcgcgt gcatcgcggg
     1921 tcacgaagcc ggcatcgcgc agcggcttgc gcagactctc gtcgtactcg atcagcgcgc
     1981 gggtgatgcc gtgacggatc gcgcccgcct ggccggtgat tccgccgccg cccacggtga
     2041 cggcgatgtc gaacttgtac acgagcttca ccgcctcgag cggttggcgc acgatcatgc
     2101 gcccggtctc gcgcccgaag aactcatcga gggagcgctc gttcaccgtg atgcgaccgc
     2161 tgcccggttt cagatagacg cgcgccgtgg cggtcttgcg ccgtccggtg ccgtaatgat
     2221 tctgctgagt cgctgccatc gcgtgcggtc ctattgaagc ttgagcggct tgggctgctg
     2281 tgcctggtga gggtgctcgc taccggcaaa tacgtgcagc ttcctgaaca tgcgccggcc
     2341 gagggggttc ttcggcaaca tccccttcac cgcgaactcg atcgcccgct cggggtgctc
     2401 gttgagcagc ttctcgagcg cgatcgactt gatgccgccg atatgacccg tgtggtgata
     2461 gtagatcttg tcgctgagct tgcgcccggt gacgcggatc ttctgcgcgt tcacgacgac
     2521 gatgtgatca cc
//
LOCUS       DATDMP010000441         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1070895, whole genome shotgun sequence.
ACCESSION   DATDMP010000441 DATDMP010000000
VERSION     DATDMP010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070895"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VI195_07720"
     CDS             complement(<1..522)
                     /locus_tag="VI195_07720"
                     /inference="COORDINATES: protein motif:HMM:NF023236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HEY6824313.1"
                     /translation="MEPATQAWWFAAGRLLGTVVIGCGVGWLLGNVWGGLACALALHL
                     AWVLANLFRLEWWLRHRSYADPPDVGGVWGEIIAQIVRLHRRKRFHKQRFVQLMRQLQ
                     RSTAALPNGVVILNAQREIVWFNRMAARLLNLRRTADLGMRIEHLMREPEFVRYLGGG
                     DYSNPVVIRTTTGE"
     gene            complement(580..1275)
                     /gene="phoB"
                     /locus_tag="VI195_07725"
     CDS             complement(580..1275)
                     /gene="phoB"
                     /locus_tag="VI195_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY6824314.1"
                     /translation="MPAKQVLIVEDERPIREMIAFGLKRAGFEVREAEDCRSARAALA
                     DTRPDLVLIDWMLPDMSGLELTRALKRDRETRELPVIMLTARAEEGDKVAGLEGGADD
                     YVTKPFSPRELLARINAVLRRGGGNGVDERIEFEGLVLDQGSHRVMVGERTVALGPTE
                     YRMLEFFMTHPERVYTRDQLLDRIWGGNVYVEERTIDVHIRRLRKALEDYGYDRFVQT
                     VRGSGYRFSARTI"
     gene            complement(1365..>2037)
                     /gene="tsaB"
                     /locus_tag="VI195_07730"
     CDS             complement(1365..>2037)
                     /gene="tsaB"
                     /locus_tag="VI195_07730"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY6824315.1"
                     /translation="DGQLTQREQWLERGHAEAILPMVDALLGEAGIRLSSLDAIAFGR
                     GPGAFTGVRLAASVTQGLAFAARLPVVPVSDLQALAQRCLDEDRAVGRVLACADARMH
                     EVYWACFERASEGMARAHGDEQVGRPSDVHLPGGWESRGPPDARPASTSGQLAGVGSG
                     FAAYPELRTALVLDALRADLLPRAAEIARLALPAVVAGRFVEPEQALPVYLRDDVIRA
                     PPKSS"
BASE COUNT          368 a          702 c          650 g          317 t
ORIGIN      
        1 ctcgccggtg gtggtgcgga tgacgaccgg gttcgaataa tccccgccgc cgaggtagcg
       61 cacgaactcg ggctcgcgca tcagatgctc gatgcgcatg ccgaggtcgg cggtgcgccg
      121 caggttgagc agccgcgccg ccatgcggtt gaaccagacg atctcgcgct gcgcgttgag
      181 gatgacgaca ccattcggca gtgccgcggt cgagcgttgc agctggcgca tgagctgcac
      241 gaagcgctgt ttgtggaagc gcttgcgccg atgcagccgc acgatctgcg cgatgatctc
      301 cccccacaca ccgccgacgt ccggaggatc ggcgtagctg cgatgccgca gccaccactc
      361 caggcggaag agattggcga gcacccaggc aaggtgcaat gcgagcgcgc aggccagacc
      421 gccccagacg ttaccgagca gccagcccac gccgcagccg atcaccacgg tgccgagcaa
      481 gcgccccgcc gcgaaccacc atgcctgtgt cgcgggttcc attgatcctg gcgtctcttt
      541 tatatggccg cgatcgtacc ggtgcggatg gtacccgtgc taaatggtcc gcgccgagaa
      601 acgataaccc gagccgcgca ccgtctgcac gaagcgatcg tagccataat cctccagagc
      661 cttgcgcaag cggcgaatgt gcacgtcgat ggtacgctcc tcgacgtaga cgttgccgcc
      721 ccagatacga tcgagcagct gatcgcgggt gtagacacgc tcggggtgcg tcatgaaaaa
      781 ctcgagcatg cggtactcgg tgggaccgag tgccacggtg cgctcgccca ccatgacgcg
      841 atgactgccc tggtcgagga ccaggccttc gaactcgatg cgctcatcga caccgttgcc
      901 gccgccgcga cgcaggacgg cgttgatgcg tgcgagcagc tcgcgcggtg agaagggctt
      961 ggtgacgtag tcgtccgccc cgccttcgag accggccacc ttgtcgcctt cctcggctcg
     1021 cgcggtcagc atgatcaccg gcaactcgcg ggtctcgcgg tcgcgcttca gtgctcgcgt
     1081 cagttcgagg ccactcatat cgggcagcat ccaatcgatc agtacgaggt cgggccgcgt
     1141 gtcggcaagc gctgcacgtg cgctgcggca atcctcggcc tcacgcacct cgaaaccggc
     1201 gcgcttgagg ccgaaagcga tcatctcgcg gatcggccgc tcgtcttcga cgatcagaac
     1261 ctgtttggcg ggcatcgtgc ctggacctgc acccggcagg agcgcggcta ttacagagcc
     1321 actgtgttgc agttaaatga caaaatcccc gaacggtgtc agtttcatga cgacttcgga
     1381 ggagcgcgta tgacatcatc gcgcaaatac accggaagtg cttgttcggg ctcaacaaat
     1441 cgtcccgcaa cgactgccgg cagggcgagc cgcgcgatct ctgcagctct cggtagcagg
     1501 tccgcacgca aagcgtcgag caccagcgcg gtgcgcagct ccgggtaggc ggcgaacccc
     1561 gagccgacac ccgcgagctg ccccgaggtt gacgcggggc gcgcgtccgg cggtccgcgc
     1621 gattcccagc cgccgggcag gtgcacatcc gacggccggc cgacctgctc atcgccgtgt
     1681 gctcgagcca tgccttcgct cgcgcgctca aagcaggccc agtacacctc gtgcatgcgg
     1741 gcatccgcgc aggccagcac ccggccaacc gcgcgatcct catccagaca ccgctgcgcg
     1801 agcgcctgca gatccgacac cggcacgacc ggcagccgtg cagcgaaagc gagaccctga
     1861 gtcacactcg ctgcgagccg caccccggtg aatgcacccg ggccgcgccc gaaggcgatg
     1921 gcatcgaggg agctcagacg gatacccgct tcgccgagca gcgcatccac catgggcaat
     1981 atcgcctccg cgtggccgcg ctcgagccac tgctcgcgtt gcgtgagctg cccatcg
//
LOCUS       DATDMP010000442         3121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_293541, whole genome shotgun sequence.
ACCESSION   DATDMP010000442 DATDMP010000000
VERSION     DATDMP010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293541"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VI195_07735"
     CDS             complement(<1..1259)
                     /locus_tag="VI195_07735"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824316.1"
                     /translation="MSEHPASGGSRIRQRQRLRLIDACISALHMYGPSRTTVEKVVAL
                     ADLSPGIVRFYFDSKAAMLVASLEHLAAEFGERVLVPAAHLKDNPVQALRLLVSLYLD
                     ADIASPRKVSVWYAFWGEASARQEYQDICGKKDEEFAALVRELMERMIAVSGASHLDA
                     DAVALGLIGVLEVLWQGIAFQSEANIDRAGAVARSLRYLSSVFPGQFSDPAAMPVRAA
                     GTSASADRLPPRAYRDEVLLAAERAQLLHPAWQVLGHEAELRAAGDYLSGDAAGVRAL
                     VVRAERGRLHAFRNTCRQRPHALVSARRGHLKSAIHCAAHSLTYSFDGRLVSGATPGD
                     LAPLQLLRAGRVLLVRAAANAAQGMTGAEESPWAAFAGLTPLQMAEQEVAADWKLLVE
                     QWLETPLPQHSFIAPNQLLEVRTDGGC"
     gene            1392..2282
                     /locus_tag="VI195_07740"
     CDS             1392..2282
                     /locus_tag="VI195_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6824317.1"
                     /translation="MRRSWAPGRWLIILPPLLFLLVFFLIPFAFAFKISFAEAAPRVP
                     PFTDIISFTPDHHLRFAISLGNYRYLLTDEVYGVAYLYSLRTAFFSTLICLALGYPMA
                     YAIARAPRSTQGLLLLIIMLPFWTSFLLRVYALEGIIRDTGFLNTALLHLGLISRPLH
                     IMRTSVAVYLGIIYSYLPFMVLPLFATLEKLDPTLLEAAADLGSPPWRAFLDITLPLS
                     IPGVIAGSMLVFIPAVGEFVIPSLLGGPNQLMIGRVLWDEFFGNHDWPVASAVAIAFL
                     LLLVGPIAIYQYYNVHQLEA"
     gene            2309..3121
                     /locus_tag="VI195_07745"
     CDS             2309..3121
                     /locus_tag="VI195_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6824318.1"
                     /translation="MALLSVLFLGIALLYVPILVLIAYSFNASPLVNVWGGFSTAWYG
                     QLLHNRQLLEGALLSLEVALAASSGAIVLGTLAAIALVRFARFPGRLLLTGMVNAPLV
                     MPEIITGITQLLLFVSMVQLLGWPHRGFTTIVLSHVTFCTAYVTITVQSRLQAADRSL
                     EEAAQDLGATPARAFVEITLPIIAPAIISSWLLCFTLSLDDLVISSFVAGPGASTLPM
                     VIYSKVKLGVSPDINALASLIVCAVGACIVAARWLMIRGERRAALEARLAAQ"
BASE COUNT          452 a         1120 c          972 g          577 t
ORIGIN      
        1 atgcaaccgc cgtcggtgcg gacctcgagc agctggttcg gtgcgatgaa gctgtgctgc
       61 ggaagcggtg tctcgagcca ctgctccacg aggagcttcc agtcggccgc gacctcctgc
      121 tcggccatct ggagcggtgt gagcccggcg aatgccgccc acggggactc ctcggcaccc
      181 gtcatcccct gagctgcgtt agcggcggca cgcaccagca gcacccggcc tgcacgcaac
      241 agctgaagcg gtgcgagatc gcccggggtc gcgcccgaga ccagccggcc atcgaagctg
      301 taggtgaggg aatgcgccgc gcagtggatg gcgctcttca gatgcccgcg gcgcgcgctc
      361 accagtgcat gcgggcgctg ccggcaggtg ttgcggaacg cgtgcaggcg cccgcgctcg
      421 gcccgcacta cgagcgcgcg cactccggcc gcatcgccgc tcaggtaatc gcctgctgca
      481 cgcagctccg cctcgtgacc gagcacctgc caggccgggt gcagcagctg cgcgcgctcg
      541 gcggcgagca gcacctcatc ccgataggca cgcggcggca gccgatcggc ggacgcggac
      601 gtgccggcgg cgcgcaccgg catagcggcg ggatcgctga actgccccgg gaacaccgag
      661 ctcaggtagc gcagcgagcg cgccacggcg ccggcgcggt cgatgttcgc ctcgctctgg
      721 aaggcgatgc cctgccacaa cacctcgagc acgccgatga gcccgagcgc caccgcgtcg
      781 gcatcgaggt ggctggcgcc gctcaccgcg atcatgcgct ccatgagctc gcgcaccagc
      841 gccgcgaact cctcgtcctt cttgccgcag atgtcctggt attcctggcg cgcgctcgct
      901 tcgccccaga aggcgtacca caccgatacc ttacgcgggc tcgcgatgtc ggcgtccagg
      961 tagagactca ccagcaggcg cagcgcctgc accgggttgt ccttcaggtg cgccgccggc
     1021 acgagcaccc gctcgccgaa ttccgccgcc aggtgctcga gcgaggccac cagcatcgcg
     1081 gccttggagt caaagtagaa acgcacgata ccgggcgaga gatcagcgag cgccaccacc
     1141 ttctccaccg tggtgcgcga gggaccgtac atgtgcagcg ccgaaatgca ggcatcgatc
     1201 agtcgcagcc gctggcgctg gcggatacgc gagccgccgc tcgcagggtg ttcgctcacc
     1261 gtggctcgct gaccggtcat ctgagtcact gctgctcctc gcactgcgcg cgtgttggcg
     1321 gcgatcggcc gctgtacggc gcgcaagttt atcccgcaca atactccgcg ggtactttca
     1381 gcacataacc tatgagacga tcctgggctc cgggccgctg gctcatcatc ctgccgccgc
     1441 tactgttcct gctcgtcttc tttctcatcc cgttcgcttt tgccttcaag atcagcttcg
     1501 ccgaggccgc cccacgggtg ccgcctttca cggacatcat ctccttcacc ccggaccatc
     1561 acctgcgctt cgccatcagc ctcggcaact accgctacct gctgaccgat gaggtctatg
     1621 gtgtcgccta tctctattcg ctgcgcaccg ccttcttctc cacgctgatc tgcctcgccc
     1681 tcggctaccc gatggcctac gccatcgccc gcgcaccccg gtccacccag gggctgctgc
     1741 tgctcatcat catgctgccg ttctggacgt ccttcctgct gcgcgtgtac gcgctcgaag
     1801 gcatcatccg cgataccggt ttcctcaaca ccgcgctgct gcacctgggc ctcatcagtc
     1861 ggccgctgca catcatgcgg acgagtgtcg cggtatacct cggcatcatc tattcctacc
     1921 tgcccttcat ggtgctgccg ctgttcgcga cgctggaaaa gctcgacccg acactgctcg
     1981 aagcggccgc ggatctcggc agcccgccgt ggcgtgcgtt cctcgacatc acgctgccgt
     2041 tatcgatccc cggggtgatc gccgggtcga tgctggtatt catcccggcg gttggagagt
     2101 tcgtcatccc gtcgctcctt ggcggcccga accagctgat gatcggccgt gtgttgtggg
     2161 atgagttctt cggcaatcac gactggccgg tggcctccgc cgtggcgatt gcgtttctgc
     2221 tgctgctggt cggcccgatc gcgatctacc agtactacaa cgtgcaccag ctcgaggcct
     2281 gacgctaccc atggagcgcc gctcatccgt ggctctgctg tcggtgctgt tcctcggcat
     2341 cgcgctgctg tacgtaccga tcctggtgct catcgcctac tcgttcaacg cctcaccgct
     2401 ggtgaacgtg tggggcggct tctcgaccgc ctggtacggg cagctgctgc acaatcgtca
     2461 gctgctggaa ggggcgctgc tgtcgctcga ggtggcgctc gccgcgtcga gcggcgcaat
     2521 cgttctcggg accctggccg cgatcgcgct ggtgcgcttc gcacgctttc cgggccgact
     2581 gctgctcacc ggcatggtga atgcgccgct ggtcatgccg gagatcatca ccggcatcac
     2641 gcagctgctg ctgttcgtct cgatggtgca gctgctcggc tggccgcacc gcggcttcac
     2701 caccatcgtg ctgtcgcacg tcaccttctg cacagcctat gtcaccatta ccgtgcagtc
     2761 gcgcctgcag gcggccgacc gctcgctcga ggaagccgca caggacctcg gcgccacacc
     2821 ggcacgcgcc ttcgtggaga tcacgctgcc gatcatcgcg ccggcgatca tctcgagctg
     2881 gctgctgtgc ttcacgctgt cgctcgatga cctggtcatc tcgagcttcg tcgcgggccc
     2941 gggtgcgagc acgctgccga tggtgatcta ctccaaggtg aagctcgggg tgagccccga
     3001 catcaacgca ctcgcgagcc tcatcgtgtg cgcggtcggg gcctgcatcg tcgccgcgcg
     3061 ctggctcatg atacgcggcg agcggcgcgc tgcattggaa gcgcggctcg ccgcgcagtg
     3121 a
//
LOCUS       DATDMP010000443         4391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_71237, whole genome shotgun sequence.
ACCESSION   DATDMP010000443 DATDMP010000000
VERSION     DATDMP010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71237"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VI195_07750"
     CDS             <1..732
                     /locus_tag="VI195_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824319.1"
                     /translation="HRFGAGFAYWCVLGGVFVTALYTFRLLFLTFHGPERFRSAHAAG
                     DEHAGHGAPPHESPAVVTVPLIALAIPSLAVGWFTAGPVLFGGYYGQSLLVLPANDVL
                     GRIAADFGGPVRFALHGLIAPPFLFALAGAVTAWASFLRRPEWAGEAARRARLLYTVL
                     THKYGFDWLYERVVAALTRGVGIGLWKGGDQGLIDGALVNGTAAAVGWFGGLVRRVQS
                     GYLYSYAFWMMIGLAVLLGWYLTHP"
     gene            745..2250
                     /locus_tag="VI195_07755"
     CDS             745..2250
                     /locus_tag="VI195_07755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY6824320.1"
                     /translation="MHHLLSMLIWLPLAAGLAVLALGERRIRAGRWLALAAALATLAL
                     TVPLWGGFDTRTAALQFVEQLPWIPRFHAYYALGVDGISLPLIVLTAFMTVPVVIAGW
                     TVIDSRPAQYYAAFLIMEGLMIGVFAASDALLFYFFWEAMLIPMFLIIGIWGGPRRVY
                     ATIKFFLYTFLGSVFMLVALIYMYVKAGNYSIAALQALPLTLGEQRGIFVTFLLAFAV
                     KVPMFPVHTWLPDAHVEAPTGGSVILAAIMLKMGGYGFLRFSLPVAPDASRELDLLVI
                     VLSLIAVIYIGFVALVQQDMKKLIAYSSIAHMGFVTLGAFVVYQIVRSTGSVQGAGMG
                     LDGAMVQMVSHGLISGALFLCVGVLYDRLHSRQIADYGGVVNTMPVFAAFMVLFALAN
                     TGLPGTSGFVGEFLVIIASFRASFWYAALAAVTLILGCCYTLWLVKRVVFGAVANPKV
                     AALADLNGREFVVLGALALAVLIVGVWPAPLLRVMEPSIQHLVTQAIATKL"
     gene            2264..3709
                     /gene="nuoN"
                     /locus_tag="VI195_07760"
     CDS             2264..3709
                     /gene="nuoN"
                     /locus_tag="VI195_07760"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY6824321.1"
                     /translation="MPATIMSFASVAPAIAEIWLVAAACAILLIDAFAGARRRGLTAT
                     LTLLALALGAALTVSFGQVAQRTVLFDGLYIADELGTVLKLAGFLLVAVALLYSRSYL
                     ENRNILRGEYYVLSLTALLGIFVLVSAGSLLTVYLGVELLALSVYALVAFDRDSGVAA
                     EAAMKYFVLGAIASGALLYGMSLIYGLSGTLDLARIAAQLHAPLSPGLILGLTFIVVA
                     VAFKLGAVPFHMWLPDVYEGAPTSVTLFIGTAPKLAYFALALRLLAFGLPGAVLEWSR
                     MLAVLAVLTLVVGNVVAIVQSNLKRMLAYSTIANVGFIVLGFVAGTPGGYGAALYYTL
                     VYVLVALGSFGVILLASSRGFEADRLDDYKGLYRRDPLLALAMMVLMFSTAGVPPFVG
                     FWAKLRIFQALWETGHLWLVVIAAAMSVVGAFYYLRVIKLMYFDPAAEGLPPTVRATG
                     VRFALGVNAVAVLALGVLPGPLLDLCARVIH"
     gene            3721..>4391
                     /locus_tag="VI195_07765"
     CDS             3721..>4391
                     /locus_tag="VI195_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824322.1"
                     /translation="MQRPLRQLVWLALLAAALGTAQAQAVPHYRIVQQLALPGDEGWD
                     YLTFEQGGHRLFVAHGSRVLVVDSDKLAVAGEIADTPGVHGIALAPDLGRGYVSAGRA
                     GTVVVFDLKTLARRGEIRVTGDNPDAILYDPATHRVFTFNGRGRNATAIDAQSDTVLG
                     TIALDAKPEFAQSDGRGRVYVNFEDRSSLALLDPRKLAIVAVWPISGCREPSGLALDA
                     ARERL"
BASE COUNT          523 a         1496 c         1555 g          817 t
ORIGIN      
        1 caccgcttcg gcgcgggctt tgcctactgg tgcgtgctcg gcggggtgtt cgtcaccgcg
       61 ctctacacct tccgcctgct gttcctcacc tttcacgggc cggagcgctt ccgcagcgcg
      121 cacgcggccg gcgacgagca cgccggccac ggcgcgccgc cgcacgagag cccggcggtg
      181 gtgaccgtgc cgctgatcgc gctcgcgatc ccgtcgctcg ccgtcggctg gttcacggcg
      241 ggcccggtgc tgttcggcgg ctactacggg cagtcgctgc tggtgctgcc ggcgaacgac
      301 gtgctcggca ggatcgccgc cgatttcggc gggccggtgc gctttgcgct tcacgggctc
      361 atcgcgccac ccttcctgtt cgcgctcgcc ggggcggtga ccgcctgggc cagcttcctg
      421 aggcgcccgg agtgggccgg cgaggcggcg cgccgcgcgc ggctgctgta cacggtgctc
      481 acgcacaaat acggcttcga ctggctgtac gagcgcgtgg tggcggcgct cacccgcggc
      541 gtcggcatcg ggctgtggaa ggggggcgat caggggctca tcgacggcgc gctggtgaac
      601 ggcactgcgg ccgcggtcgg ctggttcggc ggcctggtgc ggcgcgtgca gagcggctat
      661 ctctactcgt acgccttctg gatgatgatc gggctcgcgg tgctgctcgg ctggtacctg
      721 acgcacccct gaggagcgcg cgcggtgcac cacctgctgt cgatgctgat ctggctgccg
      781 ctcgccgcgg ggctggcggt gctcgcgctc ggcgagcgcc gcatccgtgc cgggcgctgg
      841 ctggcgctgg cggcggctct cgcgacgctc gcgctcacgg tgccgctgtg gggcggcttc
      901 gacacccgca ccgcggcgct gcagttcgtc gagcagctgc cgtggatccc gcgctttcat
      961 gcctattacg ccctcggggt cgacggcatc tcgctgccgc tcatcgtcct caccgccttc
     1021 atgaccgtgc cggtggtgat cgccggctgg acggtgatcg acagccggcc ggcgcagtac
     1081 tacgccgcgt tcctcatcat ggaaggcctc atgatcggcg tgttcgcggc gagcgacgcg
     1141 ctgctgttct acttcttctg ggaagcgatg ctgatcccga tgttcctcat catcggcatc
     1201 tggggcgggc cgcggcgcgt ctacgcgacc atcaagttct tcctctacac cttcctcggc
     1261 tcggtgttca tgctggtggc gctcatttac atgtacgtca aggcgggcaa ctactcgatc
     1321 gcggcgttac aggcgctgcc gctcaccctg ggggagcagc gcgggatctt cgtcaccttt
     1381 ctgctcgcct tcgccgtcaa ggtgccgatg ttccctgtgc acacctggct gccggacgcg
     1441 cacgtcgagg cgccgaccgg cggctcggtg atcctcgcgg ccatcatgct caagatgggc
     1501 ggctacggct tcctgcgctt cagcctgccg gtggcgccgg atgccagccg cgagctcgac
     1561 ctgctggtga tcgtgctgtc gctgatcgcg gtgatctaca tcggcttcgt ggcgctggtg
     1621 cagcaggaca tgaagaagct gatcgcctac tcctcgatcg cccacatggg cttcgtgact
     1681 ctcggggcct tcgtggtcta ccagatcgtg cgcagcaccg gcagcgtcca gggcgccggc
     1741 atggggctcg acggcgccat ggtgcagatg gtctcgcacg ggctgatctc cggggcgctg
     1801 ttcctgtgcg tcggagtgct gtatgaccgc ctgcacagcc gccagatcgc cgactacggc
     1861 ggggtggtca ataccatgcc ggtgttcgcg gccttcatgg tgctgttcgc gctcgccaac
     1921 acggggctcc cgggcacctc gggcttcgtg ggcgagttcc tggtgatcat cgcgagcttc
     1981 agggcgagct tctggtatgc ggcgctcgcc gcggtgaccc tgatcctcgg ctgctgctac
     2041 acgctgtggc tggtgaagcg cgtggtgttc ggcgcggtcg cgaacccgaa ggtggcggcg
     2101 ctcgcggacc tcaacggccg tgagttcgtc gtgctcggcg cgctggcgct cgcggtgctc
     2161 atcgtcgggg tgtggccggc gccgctgctg cgggtcatgg agccctcgat ccagcacctg
     2221 gtgacccagg cgatcgccac caagctgtga ggcgcgtgca ggcatgccgg caacgatcat
     2281 gagcttcgca agcgtcgcac cggcgatcgc cgagatctgg ctggtggccg ccgcatgcgc
     2341 catcctgctc atcgacgcgt tcgccggcgc gcggcgccgc ggcctgaccg cgaccctgac
     2401 gctgctggcg ctcgcgctcg gggcggcgct cacggtgagc ttcggccagg tggcgcagcg
     2461 caccgtgctg ttcgacggac tgtacatcgc cgacgagctc ggcacggtgc tcaagctcgc
     2521 cggcttcctg ctggtagcgg tggcgctgct ctattcgcgc agctacctgg agaaccgcaa
     2581 catcctgcgc ggcgagtact acgtgctgtc gctcaccgcg ctgctcggca tcttcgtgct
     2641 ggtgtcggcc gggagtctgc tcaccgtgta cctcggggtc gagctgctcg cgctgtcggt
     2701 gtacgcgctg gtcgccttcg accgcgactc gggcgtcgcg gcggaagctg ccatgaagta
     2761 cttcgtcctc ggggcgatcg cctcgggcgc gttgctgtac ggcatgtcgc tcatctacgg
     2821 cctcagcggg acgctcgacc tggcacggat cgccgcgcag ctgcacgcgc cgctcagtcc
     2881 ggggctgatc ctggggctca ccttcatcgt ggtggcggtc gccttcaagc tcggtgcggt
     2941 gccgtttcac atgtggctgc cggacgtgta cgagggcgcc ccgaccagcg tgacgctgtt
     3001 catcggcacc gcgccgaagc tcgcgtactt cgcgctggcg ctgcggctgc tcgccttcgg
     3061 gctgccgggc gcggtgctcg agtggtcgcg gatgctggcg gtgctcgcgg tgctgacgct
     3121 cgtggtcggc aacgtggtgg cgatcgtgca gagcaacctc aaacgcatgc tcgcctactc
     3181 gaccatcgcc aacgtcggct tcatcgtgct cggcttcgtc gccggcacgc cgggcggcta
     3241 cggcgcggcg ctctactaca cgctggtgta cgtgctggtg gcgctcggat ccttcggcgt
     3301 gatcctgctg gcgagctcgc ggggcttcga ggcggaccgg ctcgacgact acaagggact
     3361 ctaccggcgc gatccgctgc tggcactcgc catgatggtg ctgatgttct cgaccgcggg
     3421 cgtgccgccg ttcgtgggat tctgggccaa gctgcgcatc ttccaggcgc tgtgggagac
     3481 cggccacctg tggctggtgg tgatcgcggc ggcgatgtcg gtggtcggcg ccttctacta
     3541 cctgcgggtg atcaagctca tgtacttcga cccggccgcc gagggcctgc cgcccacggt
     3601 gcgcgcgacc ggagtgcgct tcgccctcgg agtcaacgcc gtcgcagtgc tggcgctcgg
     3661 cgtgctgccg ggtccgctgc tcgacctgtg tgcacgtgtc attcactgag gagaagtgag
     3721 atgcagcgcc cactccggca gctcgtgtgg ctcgcgctgc tggccgccgc actcggcacc
     3781 gcgcaggcgc aggccgtgcc gcactaccgc attgtgcagc agctggcgct tcccggggac
     3841 gagggctggg actacctcac cttcgagcag ggcggccacc ggctgttcgt ggcgcacggc
     3901 tcgcgcgtac tggtggtcga cagcgacaag ctcgccgtgg ccggcgagat tgccgatacc
     3961 ccgggggtgc acggcatcgc actcgctccc gacctggggc gcggctacgt gagcgccgga
     4021 cgcgccggta ccgtcgtggt cttcgacctg aagaccctcg cgcgccgcgg ggagatcagg
     4081 gtgaccgggg ataaccccga cgccatcctc tacgatccgg cgacgcaccg ggtattcacc
     4141 ttcaacgggc gcgggcgcaa cgccacggcc atcgatgcgc agagcgacac ggtgctcggc
     4201 accatcgcgc tggatgccaa gccggaattc gcccagtccg acggcagggg acgcgtctac
     4261 gtcaacttcg aggaccggag cagcctggcg ctgctcgatc cgcgcaagct cgcgatcgtc
     4321 gcggtgtggc cgatcagcgg ttgccgggaa ccctcggggc tcgcgctcga tgcggcgcgc
     4381 gagcggctgt t
//
LOCUS       DATDMP010000444         3007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1405364, whole genome shotgun sequence.
ACCESSION   DATDMP010000444 DATDMP010000000
VERSION     DATDMP010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405364"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VI195_07770"
     CDS             complement(<1..224)
                     /locus_tag="VI195_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824323.1"
                     /translation="MNGSGSTRKWNLRRYLVAGVLVWLPILATVWVVSFMLHLMDLTL
                     KVLPPAYRPEALVGFSLPGLGAVFALIVLLL"
     gene            complement(229..669)
                     /locus_tag="VI195_07775"
     CDS             complement(229..669)
                     /locus_tag="VI195_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY6824324.1"
                     /translation="MPFYEYECKACKYYAEVMQKISDAPLTRCPSCGKRALKKLVSVP
                     VFRLKGAGWYETDFKSDKETRRNLVGAEKEEPKAATKSETTEAKPAAAESKDSKEAKP
                     AESRTDKVASSAPVASRGRAAAPRAAARRPARTARVKSARKKRR"
     gene            complement(863..>3007)
                     /gene="clpB"
                     /locus_tag="VI195_07780"
     CDS             complement(863..>3007)
                     /gene="clpB"
                     /locus_tag="VI195_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY6824325.1"
                     /translation="EDKFQALEKYGRDLTALARQGKIDPVIGRDEEIRRAIQVLQRRT
                     KNNPVLIGEPGVGKTAIVEGLAQRIVNAEVPEGLKDKRILALDMGALIAGAKFRGEFE
                     ERLKGVLNDLAKQEGQIILFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGAT
                     TLDEYRKYLEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYEVHHKVEITDPAIVA
                     AATLSYRYITDRQLPDKAIDLIDEAAARIRMEIDSKPEALDRLERRIIQLKIEHEALK
                     KEKDEASRKRLSTLEETLRALEREYADLEEVWKAEKAAVQGTAHIREELDRARLELDA
                     ARRAGNLGRMAELQYGKIPELEKRLSSAEVAEDKAPTLVRNKVTEEEVAEVVSKWTGI
                     PVSKMLQGEKEKLLHMEDALAKRVVGQDEALRIVSDAIRRSRAGLSDPRRPNGSFLFL
                     GPTGVGKTELCKALAEFLFDTEDALIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYL
                     TEAVRRRPYSVILLDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNL
                     GSQVIQEYAGEKNYAKMKSAVMEIVQQSFRPEFINRIDDIEVFHPLGTQQIRAIVDIQ
                     LLYLRKRLQERSMDVSLDDAARDLLGQAGFDPVYGARPLKRAIQQQIENPLAQKILQG
                     EFVPGDRIRVSARDGQLSFARAEDAKRRAARG"
BASE COUNT          443 a         1006 c          969 g          589 t
ORIGIN      
        1 agcagcagca cgatgagtgc gaacacggca ccgagccccg ggagcgagaa tcccaccaga
       61 gcttcggggc ggtaggccgg tggcaggacc ttcagtgtca ggtccatgag gtgcagcatg
      121 aaactcacca cccacacggt ggcgagtatc ggcagccata cgagcacacc cgcaaccagg
      181 tagcgccgca ggttccactt cctcgtgctg ccgctgccgt tcatggtgct agcgccgctt
      241 tttgcgagcg ctcttgaccc gcgcggtgcg cgccggccgg cgggccgcgg cgcgtggcgc
      301 cgcagcacgg ccgcggcttg ccaccggggc ggagctcgcc accttgtcgg tgcgcgactc
      361 ggccggcttc gcttccttgc tgtccttgga ctccgccgcc gccggcttcg cttccgtggt
      421 ctcgctcttg gtcgccgcct tcggctcttc tttctccgcg cccaccaggt tgcgccgggt
      481 ttccttgtcc gatttgaagt cggtctcgta ccaacccgct cctttcagcc ggaataccgg
      541 caccgagacc agcttcttga gggcgcgctt gccgcaggag gggcaacggg tgagtggcgc
      601 gtcgctgatc ttctgcatga cctcggcgta gtacttgcag gctttgcact cgtattcgta
      661 gaacggcatg agctttctgg accgttggtg ttgtcacacg attataacca aggctcgccc
      721 ggcaacgacg cgcgccgccg ggcggggggc gcagcttgct ttggcgatag ttcaccgctc
      781 ggtgaaccat cggcctgacg aggtaccgac agccctcccg cgcccagagg ggcgcctggg
      841 ggcggcccct tgcacacgcg cttcagccgc gcgccgcgcg gcgcttggcg tcctcggccc
      901 tggcaaaact gagctgcccg tcgcgcgcac tgacccggat gcgatcgccg ggcacgaatt
      961 ccccctgcag gatcttctgc gccagcggat tctcgatctg ctgctggatg gcgcgcttga
     1021 gcggccgcgc gccgtacacc ggatcgaagc ccgcctggcc gagaagatcg cgcgcggcgt
     1081 catcgagcga gacatccatg ctgcgctcct gcagccgctt gcgcagatag agcagctgaa
     1141 tatcgacgat ggcgcgaatc tgctgcgtgc cgagcggatg gaacacctcg atgtcgtcga
     1201 tgcggttgat gaactcggga cggaagctct gctgcacgat ctccatgacc gcgctcttca
     1261 tcttcgcgta gttcttctcg ccggcatatt cctggatgac ctgcgagccg aggttcgagg
     1321 tcatgatgat gacggtgttg cggaagtcga ccgtgcgccc ctggccatcg gtgaggcggc
     1381 cgtcatcgag cacctgcagc agcacgttga agacatccgg atgggccttc tccacctcat
     1441 cgagcaggat cacggagtac ggtcgccgcc gcaccgcttc ggtgaggtag ccgccctcct
     1501 cgtaaccgac atagccgggc ggagccccga tgaggcgggc aaccgagtgc ttctccatga
     1561 attccgacat gtcgatgcgg atcagcgcat cctcggtgtc gaacaggaac tcggcgagcg
     1621 ccttgcaaag ctcggtcttg ccgacgccgg tgggacccag aaacaggaac gagccgttcg
     1681 ggcggcgtgg atcggataga cccgcgcgcg agcggcgaat ggcgtccgag acgatgcgca
     1741 gcgcctcgtc ctggcccacg acgcgcttcg cgagcgcatc ctccatgtgc agcagctttt
     1801 ccttctcgcc ctgcagcatc ttcgacaccg gaatgccggt ccatttggag accacttccg
     1861 cgacctcctc ctcggtcacc ttgttgcgga ccagcgtcgg tgccttgtcc tcggcgacct
     1921 cggccgagct gaggcgcttc tcgagctcag ggatcttgcc gtactggagc tccgccatgc
     1981 ggccgagatt gccggcgcgg cgcgcggcat cgagctcgag ccgggcgcga tcgagctcct
     2041 cgcggatgtg cgccgtgccc tgcaccgccg ccttctcggc cttccagact tcctcgagat
     2101 cggcgtactc ccgctcgagc gctcgcagag tctcctcgag cgtcgacagg cgtttgcgcg
     2161 aggcctcgtc cttctccttc ttgagcgcct cgtgctcgat cttgagctgg atgatgcgcc
     2221 gctcgaggcg atcgagcgcc tcgggcttgg agtcgatctc catgcggatg cgcgccgcgg
     2281 cctcatcgat gagatcgatg gccttgtcgg gcagctgacg gtcggtgatg tagcgatacg
     2341 agagcgtggc ggcggccacg atcgcgggat cggtgatttc gaccttgtgg tgcacctcgt
     2401 agcgctcctt gaggccgcgc aggatggcga tggtgtcctc caccgagggc tcatccacca
     2461 gtaccttctg gaagcgccgc tcgagtgcgg catccttctc gaggtacttg cggtactcat
     2521 cgagcgtggt agcgccgacg cagtgcagct cgccgcgggc gagcgccggt ttcagcatgt
     2581 tgccggcatc catggcacct tcggccttgc cggctcccac catggtatgt agctcgtcga
     2641 tgaacaggat gatctgacct tcctgtttgg cgagatcgtt gagcacgccc ttgaggcgtt
     2701 cctcgaactc gccgcggaac ttggcgccgg cgatgagcgc gcccatatcg agtgcgagga
     2761 tgcgcttgtc cttcaggccc tcgggcacct cggcgttgac gatgcgctgc gcaagcccct
     2821 cgacgatcgc ggtcttgccc acgccgggtt cgccgatcag caccggattg ttcttggtgc
     2881 gccgttgcag cacctggatc gcgcggcgga tttcctcgtc gcggccgatc acggggtcga
     2941 tctttccctg gcgcgccagc gcggtcagat cgcgcccgta tttctcgagc gcctggaatt
     3001 tgtcttc
//
LOCUS       DATDMP010000445         3008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_627044, whole genome shotgun sequence.
ACCESSION   DATDMP010000445 DATDMP010000000
VERSION     DATDMP010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627044"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1272
                     /locus_tag="VI195_07785"
     CDS             40..1272
                     /locus_tag="VI195_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824326.1"
                     /translation="MPSTAFASLTAVLLVVPRPGHADVTIQQQTQYDFAIIKSHGSHT
                     ELTATDKRRSDSEMHCEGLMSMFCGNAQSGEIIRLDRNVEWTLEPKKMEYRETPLPTA
                     AQLLAARQAAQAMMEKVKQCPAMAQHSAPGPDTSQCEMSPPRVDVKQPGTHATLAGHD
                     AQLTQLALTNSCTNKTTGEVCDFVLALDSWLTQDQIAGLADQKAFLQAYQAKLGIDVH
                     DPALQNQMRQFLAPYADVLKQLSAKSSELKGYPLKTVLRIAYGGEHCASAKKPPGGGG
                     ENAVADASQAAGDAAAASAAGAAGSAAGAAAGNVAKNSVGSSIFSSAASTFGSKLTSG
                     LFKKKADAPAPAANSAPDNGLPAGMIQAAQITIETTSIDASPVPPEKFEIPAGWKKVE
                     PKAKEAASKEFTCPSAGS"
     gene            1279..2061
                     /gene="xth"
                     /locus_tag="VI195_07790"
     CDS             1279..2061
                     /gene="xth"
                     /locus_tag="VI195_07790"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY6824327.1"
                     /translation="MRIATYNVNGITARLPNLLRWLEERAPDVVCLQELKAPQERFPE
                     LAIRAAGYEVIWHGQKSWNGVAILARGSAPNEIRRVLPGDPEDAHSRYIEAKVDEIVV
                     GCLYLPNGNPAPGPRFDYKLRWLERLTRHAADLIASPSPVVLAGDYNVIPTERDVYKP
                     ERWVDDALFRVETREAFARILQQGWTDAIRALHPESQIYTFWDYFRNAYQRDAGLRID
                     HLLLNPALARRLQQAGVDRDVRGWERASDHAPTWVELRASEH"
     gene            2309..>3008
                     /locus_tag="VI195_07795"
     CDS             2309..>3008
                     /locus_tag="VI195_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824328.1"
                     /translation="MATKVFWALALAPLAVSTSAISQQQPAYQPPASYQQPPAPGGYQ
                     PPPASYPQPAQPGAYQAPPPASYPPQTSGSYQQPAQPRTYQQPPPANYQTPQSAPAYQ
                     QPSVSYQAPQSAPSSPQPAANYQQPSGYQAPPPGYQTAPAGTRPPQPGGYAPQPSAAG
                     YPPSPGSYPSQPPTNYQAPTSPGGYPPAGGYPAAPSTNYPQPQGAPASYQPPQNYQQQ
                     PAGYSPAATVAPAQA"
BASE COUNT          568 a         1046 c          948 g          446 t
ORIGIN      
        1 tccaagaata ggaagaacac catgcgcgtc aggtgcacag tgccatccac cgcattcgca
       61 agcctcaccg ccgtgttgct cgtcgtgccg cggcccgggc atgcggatgt gaccatccaa
      121 cagcagacgc agtatgactt cgccatcatc aagtcacacg gcagccacac cgagctcacc
      181 gcgaccgaca agcgccgcag cgactcggag atgcactgcg aagggttgat gtcgatgttc
      241 tgcggcaacg ctcagagcgg ggaaatcatc cgcctcgacc gcaacgtcga atggacgttg
      301 gaaccgaaga agatggaata tcgcgagact ccactcccga ccgccgcgca gctgcttgcc
      361 gcccggcagg cggcgcaggc gatgatggaa aaggtcaagc agtgccccgc catggcgcaa
      421 cacagtgcgc cgggcccgga cacctcgcag tgcgagatgt cgccgccccg tgtcgatgtg
      481 aagcagcccg gcacgcacgc caccctcgcc gggcacgatg cgcagctgac gcagcttgcg
      541 ctcaccaaca gctgcaccaa caagaccacc ggggaggttt gcgactttgt cctggcgctc
      601 gacagctggc tcacgcagga ccagatcgcg ggactcgcgg atcagaaagc atttctccag
      661 gcttatcagg cgaagctcgg gatcgatgtg cacgatcccg cgctgcagaa ccagatgcgg
      721 cagtttctcg ccccgtacgc ggacgtgctg aaacaactct ccgccaagtc gagcgagctc
      781 aagggctatc cgctgaagac cgtgctgcgc attgcctacg gcggtgagca ttgcgcgagc
      841 gcaaagaagc cgccaggagg cggcggggaa aacgccgtgg ccgatgccag ccaggcggcg
      901 ggcgacgccg cggctgcttc cgctgccggc gccgcgggct cggcagcggg tgccgcagcc
      961 gggaacgtgg ccaagaactc ggtcggcagt tcgattttca gctctgcggc cagcaccttc
     1021 ggcagcaagc tcaccagcgg cctcttcaag aagaaggcgg atgcgcccgc gcccgccgcg
     1081 aacagcgcac ctgacaacgg gctgccggcg ggcatgatcc aggccgcgca gatcaccatc
     1141 gagacgacct cgatcgacgc atcgcccgtg ccgccggaga agtttgagat tcctgccggc
     1201 tggaagaagg tcgagccgaa agcgaaggag gcggcgtcga aggagttcac ctgcccgtcc
     1261 gcgggctcgt gagcgcgggt gcgcatcgcc acctataacg tcaacggcat caccgcgcgg
     1321 ctgccgaacc tgctgcgctg gctcgaggag agagctcccg atgtcgtgtg tcttcaggaa
     1381 ctgaaggcgc cgcaggagag gttcccggag ctcgccatcc gtgccgccgg atatgaagtc
     1441 atctggcacg gccagaaaag ctggaatggg gttgcgattc tcgcgcgcgg cagcgcgccg
     1501 aatgagattc gccgggtgct gccgggggac ccggaggatg cccacagccg ctacatcgag
     1561 gcgaaggtcg atgagatcgt cgtcggttgc ctgtatctgc ccaatggcaa tcccgcgcca
     1621 ggcccgagat tcgactacaa gctgcgctgg ctcgagcggc tgacgcgtca tgcggccgat
     1681 ctcatcgcga gcccctcgcc cgtggtgctc gccggcgact acaacgtcat cccgaccgaa
     1741 cgggacgtct acaagcccga gcgctgggtg gatgatgcgc tcttccgcgt cgagactcgg
     1801 gaagccttcg cgcggatctt gcagcagggc tggacagatg cgattcgagc cctgcatccc
     1861 gagtcgcaga tctacacctt ttgggactat ttccggaacg cctaccagcg cgatgccgga
     1921 ctgcgtatcg atcacctgct gctgaacccc gccctcgcgc ggcgcctgca gcaggctggg
     1981 gtcgatcgcg atgtacgtgg ctgggagcga gcgagcgatc acgcgccgac ctgggtcgag
     2041 ctgcgcgcga gcgagcattg aggctgggtc gccgctagcg agacatgacg attgaacaga
     2101 aagccgtggc ttgatctcca cggtatgcgc cgcaggaaat ttttcggccg ggcgggcttc
     2161 ggaacaggaa ctctcctaat ccggtaagca tcaatcgtcg tgctgcgtta attttcgtta
     2221 ccgcaggggg ctggcgtgcc tcggcacggc agcgctcggc gacaaagggg gtgccatggg
     2281 ggtgctccac cgagtcggga gcgacggtat ggctacaaaa gttttctggg cgctcgcgct
     2341 agcgccgctt gcagtctcga cgtccgcaat ctcgcagcag cagccggcgt atcagccgcc
     2401 cgcgagctac caacagccgc cggctcccgg cggctatcag ccgcctcccg cgagctaccc
     2461 gcagcccgcg caacctggcg cgtatcaggc gcccccgcct gcgagctacc cgccgcagac
     2521 ttccggcagc tatcagcagc cggcgcagcc gcgtacctat cagcaaccac cgccagccaa
     2581 ttaccagacc ccgcaatccg cgccggctta tcagcagccc tcggtaagct accaggcgcc
     2641 gcaatcggca ccgtcctccc cacagccggc ggccaattat caacagcctt ccggctatca
     2701 ggcgccgccg ccgggatatc agaccgcgcc ggccggtacc cggccacctc agcccggcgg
     2761 ctacgcgccg cagccctccg cggccggtta tccgccctca ccgggcagct atccgtccca
     2821 gccgccgacc aactaccagg cgcctacttc acccggcggc tacccgccag ccggcggcta
     2881 tccggcagcg ccctcgacga attacccgca accgcagggt gcgccggcct cctatcagcc
     2941 gccgcagaac taccagcagc agcccgccgg ctactcgcca gcggctaccg tcgcacccgc
     3001 acaagcgg
//
LOCUS       DATDMP010000446         2422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1071826, whole genome shotgun sequence.
ACCESSION   DATDMP010000446 DATDMP010000000
VERSION     DATDMP010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071826"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VI195_07800"
     CDS             complement(<1..260)
                     /locus_tag="VI195_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY6824329.1"
                     /translation="MTRKVQVHQVDAFTREPFTGNPTGVVLNADALSEAQMLAIAREL
                     NNAETAFILAPDADDHTVRARFFTPRSEAGFVGHATIAAQYVL"
     gene            382..642
                     /locus_tag="VI195_07805"
     CDS             382..642
                     /locus_tag="VI195_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HEY6824330.1"
                     /translation="MDIGVEISLYPLKADFVPAIHEFLERLNGAAELRVVTNSMSTQV
                     FGPYDAVMRALRAELRTTFEALANAQDKAVFVMKVLGPLPPA"
     gene            752..1066
                     /locus_tag="VI195_07810"
     CDS             752..1066
                     /locus_tag="VI195_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6824331.1"
                     /translation="MALLRVVDRDGVEHEVEAKTGLKVMENLRELDYGVAAICGGMCS
                     CATCHVYVDPDWQTRLPAMMSDERELLAELTHHQDNSRLSCQLEFTPELAGLRVRIAP
                     DE"
     gene            1070..1864
                     /locus_tag="VI195_07815"
     CDS             1070..1864
                     /locus_tag="VI195_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY6824332.1"
                     /translation="MSAPVVKVETRGAVALVTLNRPGSGNTLNLQVAMDLLAAAMTCA
                     RTAAVRAVVLTGAGRHFCFGGDLRAFGTRAPGGDDYIRELTTYLHAAISRFVRMDAPV
                     IAAVNGTAAGAGVGLVAMADLALCGRSSKFNLAYTQAGLTPDAGTTFLLPRTLGLKRT
                     MELLLLNRSLPAEEALSWGLVNEVVADEQLLPRTLEIAEQLARGAGGAFGASKRLLAH
                     SVGALESQMVLESETIARHAVGAEGREGVSAFLEKRKPRFPDIDSG"
     gene            1880..2275
                     /locus_tag="VI195_07820"
     CDS             1880..2275
                     /locus_tag="VI195_07820"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEY6824333.1"
                     /translation="MSFTVLLLAAALALIALRIYFKLRTSRKLRAETWDEQVIGRLRS
                     QGYAPFRDYRVDFFLALPNEAACAGARARLEPEFSVDVKPLESDSELAYSLHATKTMR
                     LVVPDMQEISRRLSALAQELHGRYDGWAA"
BASE COUNT          379 a          770 c          863 g          410 t
ORIGIN      
        1 agcacatatt gcgcggcgat ggtcgcatgc ccgacgaacc cggcctcgct gcgcggagtg
       61 aagaagcggg cgcgcaccgt gtgatcgtcg gcatccgggg cgaggatgaa ggcggtctcg
      121 gcattgttca gctcgcgcgc gatggcgagc atctgcgcct ccgagagcgc atcggcgttg
      181 agcaccacgc cggtcggatt gccggtgaag ggctcacggg tgaacgcatc cacctgatgg
      241 acctggacct tgcgcgtcat gtgctcatcc gccgtttcca tcccgagcgg ccaattctag
      301 ccccgggcct gcgatcatag ccgcagccgg cgtggggtgc gcgtgcaata ctgacgtcaa
      361 cgtcgagcgg aggcggcacg tatggacatc ggtgtcgaga tcagcctgta tcccctgaaa
      421 gcggacttcg ttcccgcgat tcacgagttt ctcgagcgtc tgaacggtgc cgcggagttg
      481 cgcgtggtga ccaacagcat gagtacccag gtattcgggc cgtacgatgc ggtgatgcgg
      541 gcgctgcgcg ctgagctgcg cactaccttc gaggctctgg cgaacgcaca agacaaggcc
      601 gtgtttgtga tgaaggtgct cgggccgctg ccacccgcat gacaggcgcg ccggtcctct
      661 ggcgggccgc agggcgaagc ggcaaactag cagatcccgg gcgctcgcgc cgcaacgcat
      721 tccgattttt gtttggggga taggggacag catggcgctc ttgcgagtcg tggatcgcga
      781 cggggtcgag cacgaagtcg aggccaagac cggactgaag gtgatggaga acctgcgcga
      841 gctcgattac ggcgtggcgg cgatctgtgg cggcatgtgc tcctgtgcga cctgtcacgt
      901 atacgtcgac ccggactggc aaacgcgctt gccggcgatg atgtcggatg agcgcgagct
      961 gctcgccgag ctcactcacc accaggacaa ctcgcgcctg tcctgccagt tggagttcac
     1021 gcccgagctc gccgggctgc gtgtccgcat cgctcccgat gagtaggcca tgtccgctcc
     1081 ggtagtgaag gtcgagaccc gtggcgcagt ggctctggtc acgctcaacc gacccgggag
     1141 cggcaacacg ctcaatctgc aggtggcgat ggatctactc gctgccgcca tgacctgcgc
     1201 gcgcaccgcc gcggtgcgcg cggtggtgct taccggagcg ggcaggcatt tttgcttcgg
     1261 cggggatctg cgcgccttcg ggacgcgcgc gccggggggc gatgactaca tccgcgagct
     1321 caccacctac ctgcatgccg cgatctcacg gttcgtgcgc atggacgcgc cggtgatcgc
     1381 cgccgtgaac ggcacggcgg cgggtgcagg agtgggcctc gtggcgatgg cggatcttgc
     1441 gctctgcggg cgcagctcga agttcaacct ggcctacacc caggcggggc tcacgcccga
     1501 tgccggcacc accttcttgc tgccgcgtac cctcggactc aagcgcacca tggagctgtt
     1561 gctgctcaat cgcagcctgc cggcggagga ggcgctttcc tggggtctgg tcaacgaggt
     1621 ggtggccgat gagcagctgc tgccacgcac gctggagatc gcggagcagt tagcccgcgg
     1681 cgcaggcggc gccttcggcg ccagcaagcg tctgctcgcg cattcagtcg gtgcgctcga
     1741 gtcgcagatg gtgctcgaga gtgaaaccat cgcccggcac gcggtgggtg cggagggcag
     1801 ggagggagtc agcgccttcc tcgagaagcg caagccgcgc tttcccgaca tcgactccgg
     1861 ctagcgggca gccgcgccca tgagcttcac cgtcctgctg ctcgccgcgg cgctcgcgct
     1921 gatcgcgctg cgcatctact tcaagctgcg cacctcacgc aagttgcgtg ccgagacctg
     1981 ggacgagcaa gtgatcggcc ggctgcgctc acagggctac gctcccttcc gggactatcg
     2041 ggtggacttc tttctggcgc tgccgaacga ggcggcctgc gcgggggcgc gcgcgcggct
     2101 ggagcccgag ttcagcgtcg acgtgaagcc gctcgagagt gactcggagc tcgcctacag
     2161 cctgcacgcg accaagacca tgcggctggt agtgccggac atgcaggaaa tcagccggcg
     2221 tctgagcgca ctcgcgcagg agctgcacgg gcgctatgac ggctgggcgg cgtagcgcgc
     2281 tcacgcgcgc tggtcagcgg ggcttctcgg tctggcgctt gacggcttcc gcggcggcgg
     2341 cgatggcggc gggatcaccg aggtagcggc tgccgcgggg gcgaagctcg ctgtcgagct
     2401 cgtagagcag cggcacgccg gt
//
LOCUS       DATDMP010000447         4754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_405234, whole genome shotgun sequence.
ACCESSION   DATDMP010000447 DATDMP010000000
VERSION     DATDMP010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405234"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VI195_07825"
     CDS             complement(<1..1210)
                     /locus_tag="VI195_07825"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY6824334.1"
                     /translation="MLLLLGLPVAFSFFVINLVGAQLFLGGEAGLIQIVRNSVVSVAS
                     FSLTPIPLFVLMGEVLFHTGLAVKVIDGVERLIRQVPGRLAVVAVVAGTVFSAISGST
                     IATTAMLGSLMLPVMLSRGYHPTMATGPLMAIGAVDMLIPPSALTVLLGSLSGVSISK
                     LLIGGVLPGLILSAAFIVYIVVRVKLKPSLAPTTGFTEYHGWEKLRPFFQYVVPLVSI
                     FVVVVASMSAGWATPTESAAIGALFTMVLALAYRALSPGNLLKALRGTAAISGMILFI
                     IVGATTFSQILSFSGASNGLVEIIAGLGLAPGAVIIAMMLLLVFLGLFVEQVSMMMIT
                     LPIYMPLVQKYGVDPVWFGVMFLICMQLGLLLPPHGLLLMTMRGVAPPAVTMAHIFRA
                     VVPYIALSLIL"
     gene            complement(1260..1823)
                     /locus_tag="VI195_07830"
     CDS             complement(1260..1823)
                     /locus_tag="VI195_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY6824335.1"
                     /translation="MERAASSAGTTAAGHAAVVFGRLLEALALLGCAVIFAMTLMICA
                     DVLLRNVRLVPGVYGLAWANEISEAMLYLVTLLVAPWLLRQGQHIRVDIVLRAVPPRL
                     GWYFEWVADAMGLACCLAIAWYGLVATMKSYQSGALSIKTLVTPEWWMQAPLPVAFLL
                     LSVEMGFRMRRLYLGERAPRDDAVSTG"
     gene            complement(1823..2776)
                     /gene="dctP"
                     /locus_tag="VI195_07835"
     CDS             complement(1823..2776)
                     /gene="dctP"
                     /locus_tag="VI195_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY6824336.1"
                     /translation="MKRLVCIAAAAAAPLLAQAQEVTLRLVSAFPENQFYVKRTVDWI
                     ADVNKDGKGILQINFIGGPKAIPTFEVGKAVQSGVVDIGFSTGAFYTNVMPEADILKL
                     SETSAAEQRKNGGYDLINRIWAEKGNMRYLAKVVEFTPFHLYINKKIDKPDLTGLKIR
                     ITPVYREFFQSMNAQVMTTAPGEVYTALERGVIDGYGWPIHALFDLNWQEKTKFRVDP
                     GFYNAEVGLIMNLDKYKSLTPAQREYLDRKTLAYEGQNDFWKKYNQEEAARQKQAGIE
                     TITFDAATSKAYVEKAKEIGWANAIKASPQYGPELKKVLAR"
     gene            2864..3763
                     /locus_tag="VI195_07840"
     CDS             2864..3763
                     /locus_tag="VI195_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6824337.1"
                     /translation="MNVAGLDLNLLRVFDAVLRDRSVTVAARHLGLTQPAVSNALARL
                     RAQFEDALFVRSSTGMDATPFARQLAEPVRQALALLESALAHGPGFDPESATRAFRFY
                     MSDLGQVEFLPPLVERAQRIAPGVRLEAVALEVEDIGDALATGAIDIAVGFLPGLGPP
                     ARRQPLFRDPYVCLMRADHPAARTRLTKKAFLDASHALVSYKGGHRVIEEALERAGLA
                     RKIALRVPHFTVVPMVLERSDLILTLPSRIARVYERQGRFKYLPPPVPIPPADVAAHW
                     HERFERDPGNRWLREMIRELFTE"
     gene            3780..>4754
                     /locus_tag="VI195_07845"
     CDS             3780..>4754
                     /locus_tag="VI195_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6824338.1"
                     /translation="MKMLIAGAGPAGLYLGYLVKRQLPGWEVRIVEQNARDSTFGFGV
                     VFSERALEFLRADDPQTYERITPAMEAWSDITVVHRGTPVVIDGIGFSAIGRLKFLQL
                     LRRQLEDSGVSPEYDFALREEQDLHEFDLVVAADGANSTIRKTADFGTTVTALSNKFA
                     WFGTTRAFDTLTQTFVQNEAGAFNAHHYRHSPAMSTFVFECDAATWRRAGFERMGEAE
                     TLAYCEGVFADALQGHRLVSNKSIWRNFPKVRNRRWSAGHKVLIGDAQRTAHFSIGSG
                     TRLAMEDAISLVKVLMEHKKGIPEALAAFEALRRPVVEKLVAAADASAE"
BASE COUNT          803 a         1591 c         1596 g          764 t
ORIGIN      
        1 gcaggatcag gctgagcgcg atatagggca ccacggcgcg gaagatgtgc gccatggtga
       61 cggcgggcgg cgccacgccg cgcatggtca tgagcagcag gccgtgcggc ggcagcagca
      121 gtccgagctg catgcagatg aggaacatca cgccgaacca gaccggatcg acgccgtact
      181 tctgcaccag cggcatgtag atcggcagcg tgatcatcat catgctcacc tgctccacga
      241 acaggccgag gaagaccagc agcagcatca tcgcgatgat caccgcgccc ggcgcgaggc
      301 cgaggccggc gatgatctcc accaggccgt tgctggcgcc ggagaaggac aggatctgcg
      361 agaacgtggt ggcgcccacg atgatgaaaa ggatcatccc ggaaatggcg gcggtgccgc
      421 gcagtgcctt cagcagattg cctgggctga gcgcccgata ggcgagcgcg agcaccatgg
      481 tgaagagcgc gccgatggcc gccgattcgg tcggcgtcgc ccagcccgcc gacatcgagg
      541 cgaccaccac gacgaagatc gacaccagcg gcacgacgta ctggaagaag gggcgcagct
      601 tctcccagcc gtggtattcc gtgaagccgg tggtcggcgc gagcgagggc ttcagcttca
      661 cgcgcacgac gatgtagacg atgaacgccg cgctcaggat caggcccggc agcacgccgc
      721 cgatcagcag cttggagatc gatacgcccg acaggctgcc gagcagcacc gtcagcgcgg
      781 agggcgggat cagcatgtcg acggcgccga tcgccatcag cggccccgtg gccatggtgg
      841 ggtggtagcc gcgcgacagc atcaccggca gcatcaggct gcccagcatg gccgtggtgg
      901 cgatcgtcga ccccgagatc gcggagaaca ccgtgccggc gacgacggca accaccgcga
      961 ggcggcccgg cacctggcga atgagcctct ccacgccgtc gatcaccttc accgcgagcc
     1021 ccgtgtggaa cagcacctcg cccatcagca cgaacagcgg gatcggcgtc agcgagaagc
     1081 tcgcgaccga caccacgctg ttgcgcacga tctggatgag gccggcctcg ccgcccagga
     1141 acagctgggc gccgaccaga ttgatgacga agaacgagaa cgcgaccggc aggccgagca
     1201 gcagcagcac ggtcgagccg ccgagcatga gccaggccgc ggcctcccag gacatcggct
     1261 cagcccgtcg acaccgcgtc gtcgcgcggg gcgcgctcgc cgaggtagag gcggcgcatg
     1321 cgaaatccca tctcgaccga cagcagcagg aacgccaccg gcagcggcgc ctgcatccac
     1381 cattcgggcg tcaccagcgt cttgatcgac aacgcgcccg actggtagct cttcatggtg
     1441 gcgaccaggc cgtaccaggc gatggccagg cagcaggcga gccccatcgc gtcggcgacc
     1501 cattcgaaat accagccgag ccgcggcggc acggcgcgca gcacgatatc cactcggatg
     1561 tgctggccct ggcgcagcag ccagggcgcg accagcagcg tgacgaggta gagcatcgcc
     1621 tcggagattt cgttggccca ggcgaggccg tacacgccag gcaccaggcg cacgttcctc
     1681 agcaggacgt cggcgcagat catcagcgtc atcgcgaaga tgacggcgca gccgagcagc
     1741 gccagcgcct cgagcagcct gccgaaaaca acggccgcgt gtccggcggc cgtggtccct
     1801 gcgctcgacg ccgcccgttc catcagcgcg cgagcacctt cttcagctcg gggccgtact
     1861 gcggcgaggc cttgatggcg ttcgcccagc cgatttcctt cgccttctcg acgtaggcct
     1921 tgctggtcgc ggcatcgaag gtgatggtct cgatgccggc ctgtttctgc cgcgccgcct
     1981 cttcctggtt gtacttcttc cagaaatcat tctgcccctc gtaggcgagc gtcttgcggt
     2041 cgaggtattc gcgctgcgcc ggcgtgagcg acttgtactt gtccaggttc atgatcagcc
     2101 cgacttccgc gttgtagaag cccgggtcga cgcggaattt ggtcttctcc tgccagttga
     2161 ggtcgaagag cgcgtgaatc ggccagccat agccgtcgat cacgccgcgc tcgagcgccg
     2221 tgtacacctc gccgggcgcg gtggtcatga cctgcgcgtt catcgactgg aagaactcgc
     2281 ggtacaccgg cgtgatgcgg atcttcaggc cggtgaggtc gggcttgtcg atcttcttgt
     2341 tgatgtacag gtggaagggc gtgaattcga ccaccttggc gaggtagcgc atgttgccct
     2401 tctcggccca gatcctgttg atcaggtcgt agcccccgtt cttgcgctgc tcggccgcgc
     2461 tcgtctccga aagcttgagg atgtcggcct cgggcatcac gttggtatag aaggccccgg
     2521 tgctgaagcc gatgtcgacc acgccggact gcaccgcctt gccgacctcg aaggtgggaa
     2581 tggctttcgg cccgccgatg aagttgatct gcaggatgcc cttgccgtcc ttgttcacgt
     2641 ccgcgatcca gtcgacggtg cgcttcacgt agaactggtt ttcggggaat gcgctcacca
     2701 ggcgcagcgt gacttcctgg gcctgggcga gcaggggtgc ggccgcggcc gcggcgatgc
     2761 aaacgagtct cttcatggct cctcctcctg taaggagcgg aaagggtagg gcgcgggaca
     2821 atcatttgtc aaatcaatgt cattcatgct attaatcatt tctgtgaatg tcgcgggcct
     2881 cgacctgaac ctgctgcgcg tgttcgacgc ggtgctccgc gaccgcagcg tgaccgtagc
     2941 cgcgcgccac ctgggtctca cgcagccggc ggtgtcaaac gcgctggcgc ggctgcgcgc
     3001 gcagttcgag gacgcgctgt tcgtgcgctc ctccaccggc atggatgcga cgccgttcgc
     3061 gcgccagctc gccgagccgg tacgccaggc gctcgcgctg ctcgaatcgg cgctcgcgca
     3121 cggtccgggc ttcgacccgg agagcgccac gcgcgcgttc cgcttctaca tgtcggacct
     3181 gggccaggtc gagttcctgc cgccgctggt ggagcgcgcg cagcgcatcg ctccgggcgt
     3241 gcgcctggag gcggtggcgc tggaggtcga ggacatcggc gatgcgctcg ccaccggcgc
     3301 gatcgacatc gcggtgggct tcctgcctgg gctgggcccg ccggcgcgcc gccagccgct
     3361 gttccgcgat ccctacgtct gcctgatgcg cgccgatcat ccggccgcgc gcacgcgcct
     3421 gaccaagaag gcgtttcttg acgcctcgca tgccctggtg tcctacaagg gcgggcaccg
     3481 cgtgatcgag gaggcgctcg agcgcgccgg cctcgcacgc aagatcgcgc tgcgcgtgcc
     3541 gcatttcacc gtcgtgccga tggtgctgga gcgcagcgac ctgatcctca cgctgccctc
     3601 caggatcgcg cgcgtctacg agcgccaggg ccgcttcaag tacctgccgc cgccggtgcc
     3661 gattccgccc gccgacgtcg ccgcccattg gcacgagcgc ttcgagcgcg atccgggaaa
     3721 ccgctggctg cgcgagatga tccgcgagct gttcacggaa tagcgggaga atcgcggcga
     3781 tgaagatgct gatcgccggc gccgggccgg ccgggctgta cctcggctac ctcgtgaagc
     3841 gccagctgcc gggctgggag gtgcgcatcg tcgagcagaa cgcgcgcgac tcgacgttcg
     3901 gcttcggcgt ggtgttctcc gagcgcgcgc tcgagttcct gcgcgccgac gacccgcaaa
     3961 cctacgagcg catcacgccc gcgatggaag cctggagcga catcaccgtc gtgcaccgcg
     4021 gcacgccggt ggtcatcgac ggcatcggct tctcggcgat cggccggctg aaattcctgc
     4081 agctgctgcg caggcagctg gaagactcgg gggtttcacc cgagtacgat ttcgcgcttc
     4141 gcgaggaaca agacctgcac gagttcgatc tcgtggtcgc ggcggacggc gccaattcca
     4201 cgatccgcaa gaccgccgat ttcggcacga cggtcacggc gctcagcaac aagttcgcct
     4261 ggttcggcac gacgcgcgcg ttcgacacgc tcacgcagac tttcgtgcag aacgaggcgg
     4321 gcgcgttcaa cgcgcaccac taccggcact cgcccgcgat gagcaccttc gtgttcgagt
     4381 gcgatgcggc cacctggcgg cgcgccggct tcgagcgcat gggcgaggcc gagacgctcg
     4441 cctattgcga gggcgtgttc gccgatgcgc tgcagggcca tcgcctggtg tcgaacaagt
     4501 cgatctggcg caacttcccc aaggtgcgca accggcgctg gtcggcgggc cacaaggtgc
     4561 tcatcggcga cgcccagcgc acggcgcact tctcgatcgg ctccggcacg cgccttgcca
     4621 tggaagacgc aatttccttg gtcaaggttc ttatggagca taaaaaaggc attccggaag
     4681 cgctcgccgc cttcgaagcc ttgcgccggc cggtcgtcga gaagctggtc gccgcggcgg
     4741 atgcgagcgc cgag
//
LOCUS       DATDMP010000448         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_849491, whole genome shotgun sequence.
ACCESSION   DATDMP010000448 DATDMP010000000
VERSION     DATDMP010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849491"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1447
                     /gene="istA"
                     /locus_tag="VI195_07850"
     CDS             188..1447
                     /gene="istA"
                     /locus_tag="VI195_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY6824339.1"
                     /translation="MLTAGELFMILDLHRQGLSVSAIARRLDLDRKTVRKYIARGLEP
                     PVYGPRAARPQKADAYLEFLRDRLRAFPQLTATRLLREIQPLGFSGSYSTVKSAIREL
                     RPAPALGFEHRFETAAGEQAQVDFAQFRTAFADDPQQSITLWLFTLVLGYSRYLWGEF
                     VWHQDLLTVLRSHVRAFAALGGVPGEILYDRMKTAVLDEVSEGIIYNARLQGLAKHYG
                     FTPRACAAYRAKTKGKVERPYRYIRQDFFLGRAFRDLEDLNTQFREWLDTVANRRRHG
                     TTHRPIAAAFIAEQGALQALPQLPFNTVMVLERAYSRDGMVSYNGNAYSVPDGVRGQR
                     VEIQVSLSEVQIFADGKLVAAHALREGRNERSFASGHRRWPPPGTRASLQESAAAVLR
                     LPGEQVARRSLEVYDRIGTALATGARR"
     gene            1444..>2052
                     /locus_tag="VI195_07855"
     CDS             1444..>2052
                     /locus_tag="VI195_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007396167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824340.1"
                     /translation="MNAAAPTAIVDRIRRHLVALKMPTAIERLDALVQRFEQGQINPY
                     ELLEQLLAEERHLRENRRIKMALLTARLTTLKTLESFDFSFQPSIDRNRILSLAQLQF
                     IERREVVHFLGPPGTGKSHLAVALGLEAVKIGKSVYFTTLGELVSGMAKAEREGQLKE
                     RISFYTRNALLIIDEIGYLPLQSGGANLFFQLINARYERGAMI"
BASE COUNT          366 a          668 c          637 g          381 t
ORIGIN      
        1 cggcagatcg acgggctggc gatcctggcg cgggatgtga ttgaggcgga tccactttcg
       61 ggcgcgatgt ttgccttctg tcgatgtcgg agcgatattc cccagaagtg gcagagtaat
      121 tttccccacc tatcgaggtt aagcacgata tgcccggcgt ccctgggagg gcgcgacagg
      181 aggctgcgtg ctcactgctg gggagttatt catgattctg gatctgcatc gacagggttt
      241 atcggtctcg gcgattgcgc gacggctgga tctggaccgc aagacggtgc gcaagtacat
      301 cgcacgaggt ctggaaccgc cggtctatgg cccgcgggca gctcggccgc agaaggccga
      361 tgcgtatctt gagtttttgc gggatcggct gcgcgctttt ccgcagctga cggcgacacg
      421 actgttgcgc gagatccaac cactgggctt ctcgggcagt tacagcacgg tgaagagcgc
      481 gatcagggag ttacggccgg cgccggcgct gggctttgaa catcgcttcg agaccgccgc
      541 cggcgagcaa gcgcaggtgg acttcgcgca gtttcggacc gcctttgccg atgatcccca
      601 gcagagcatc acgctgtggc tcttcactct cgtgctcggc tacagccgct atctgtgggg
      661 tgagttcgtt tggcaccagg acctgctcac ggtgctgcgc tcgcatgtgc gtgcctttgc
      721 cgccttgggc ggcgtgcccg gagagattct ttacgatcgc atgaagaccg ccgtgctcga
      781 tgaagtcagc gagggcatca tctataacgc ccgcttgcag ggactcgcca agcactacgg
      841 cttcacaccc cgggcctgcg ccgcctaccg cgccaagacc aagggcaagg tcgagcgacc
      901 gtatcgctac attcgtcagg attttttcct gggccgagcc ttccgcgatc tggaggatct
      961 caacacgcag tttcgcgagt ggctcgatac ggtggcgaac cgccgccgcc atggcaccac
     1021 tcaccgaccg atcgccgccg ccttcattgc tgaacagggt gcactgcagg ccttgcccca
     1081 gctgccgttc aacaccgtca tggtgctcga gcgcgcttac agccgcgacg gcatggtcag
     1141 ctacaacggc aacgcctact cagtccccga cggagttcgc ggccagaggg ttgagatcca
     1201 ggtctcctta agcgaggtgc agatcttcgc cgacggcaag ctcgtggccg cgcacgcgct
     1261 gcgcgaaggc cgcaacgaac gcagcttcgc cagcgggcat cggcgctggc cgccacccgg
     1321 cacgcgcgcc tctctccagg agtctgcagc cgctgtattg agattgcccg gcgagcaagt
     1381 cgcgcgccgc tcgctcgagg tctacgatcg cattggcaca gcattggcaa ccggagcgcg
     1441 gcgatgaacg ccgcggctcc gacggccatt gtcgaccgca tccgccgcca cctggtggcc
     1501 ttgaagatgc ccaccgccat cgagcggctc gatgcgctcg tgcagcgctt cgagcagggg
     1561 cagatcaacc cctatgagct gctcgagcaa ctgctggccg aggaacggca cttgcgtgag
     1621 aaccggcgca tcaagatggc gctgctgacc gcgcgcctca ccacactcaa gaccctcgag
     1681 agctttgatt tcagcttcca gccctcgatc gatcgcaacc gcatcctctc cctcgcccag
     1741 ctgcagttca tcgaacggcg cgaagtggtg catttcctcg gcccacccgg taccggcaag
     1801 tcgcacctgg cggtcgccct ggggctcgaa gccgtcaaga tcggcaagag cgtgtacttc
     1861 acgaccctcg gggaacttgt cagcggcatg gccaaagccg agcgcgaggg acagctcaag
     1921 gagcgcatca gcttctacac ccgcaacgcg ctgctgatca tcgatgagat cggttactta
     1981 ccgctgcagt caggcggcgc gaacctgttc ttccagctca tcaacgcccg ctacgagcgc
     2041 ggcgccatga tc
//
LOCUS       DATDMP010000449         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1961050, whole genome shotgun sequence.
ACCESSION   DATDMP010000449 DATDMP010000000
VERSION     DATDMP010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961050"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VI195_07860"
     CDS             <1..309
                     /locus_tag="VI195_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824341.1"
                     /translation="AERDAQGLDYERLSRSISEHLSQACEPDGVPMRALRRLQLRRHA
                     RTAPRGFRASRSADLPGRENEAFDMCTALIKAARRWYARSACHDPTVQANLARLAVLR
                     "
     gene            575..1609
                     /locus_tag="VI195_07865"
     CDS             575..1609
                     /locus_tag="VI195_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY6824342.1"
                     /translation="MQRIPVLERGSRPPLRVGIVLAHHFTLSAFAVFVDHLRLAADEG
                     DRSRPLHVQWSIMASRAEPVPASCGVMLQPTHGLLAPETLDYVVVIGGILHAGPQVDE
                     TTTRYLREVGTTSVPLIGICTGSFILCRAGLMKGRRSCVSWYHYQDFLEAFPNQEVIA
                     DRLFLADGPRITCAGGAGAAALATYLIEQHLGRAVAQKASQVLLFDRTRSGSDAQPHP
                     PLSESVAEPRVRRALLLMEQNLARPIAVAAVAAELGLSARQLERLCRQHVGMGPASLY
                     RQLRMRYANWLIENTDRSVTDIANEAGFADCAHFSRQFKDAYGLSPTSRRLQPQRVAS
                     GDHARARVFE"
     gene            1798..>3509
                     /locus_tag="VI195_07870"
     CDS             1798..>3509
                     /locus_tag="VI195_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6824343.1"
                     /translation="MKSKISYAVAAILGGTSVAASAQEQAAGTTEATSEGIAEVVVTA
                     QRRSEKMQDVPISMQALTGQALQQLNITTFDDYLKYLPNVTSASNGPGQNEVFMRGLS
                     AGSQASQGSGSTGLWPNVAIYLDNQSGQLPNRNLDVYAADIDRIEVLAGPQGTLFGAG
                     AEAGVIRYITNAPKLDRTEGSVTASYGVTAGGDPNTAVQAVLNLPLIEGKMAARAVIY
                     NDRRGGYIDNVPATFTRKNTDIGIGFAYLPATGGVCPDGLPNNGFCVPPGSPVLNNYL
                     IARNNINPVSYQGIRAELLYKFSDDWDVLITQSYQDMHSQGVFYQQPFASDGAPLQPL
                     QVTLFNTADTKDKFESTAWTVNGKFGDIRAVYTGGYLVRNIDQTGDYTNYARGVYADY
                     YQCYGPGAYSPNLPPKCFSPSAGWHSVEQNKHFQNELRFSTPDEWRLRGILGAFWEDN
                     QLFDQTAWMYKELPPCTSSGTAGVDPGNTGCLSNVGSIPGTTVVNPGVQNDRTSFYQD
                     QVREYKQLAFFASLDFDLIPKTLTVTAGTRYFRFDNSMKGGVLSSFGCFQQGLNGFIA
                     TGAT"
BASE COUNT          651 a         1151 c         1081 g          626 t
ORIGIN      
        1 gccgagcgcg atgctcaggg cctcgactac gaacgcctct cgcgcagcat cagcgagcac
       61 ctgtcgcagg cctgcgagcc ggacggcgtg ccgatgcgcg cgctccggcg cttgcagttg
      121 cgtcgtcacg cacgcacggc gccgcgcggt tttcgtgcga gccgctcggc agacctgccg
      181 gggcgggaga atgaagcctt cgacatgtgc acggcgctca tcaaggccgc gcgacgctgg
      241 tacgcgcgca gcgcctgcca cgatcccacc gtacaggcga atctcgcgcg gctcgcggtg
      301 ctgcgctgag tgcaagctcg ctggataacg cgcgcattcg cagcttcttg cgtccataac
      361 tgcacgcagt gcgcgagcat cgcgccctga acaggcgttc aaaacacctg ccacgtggcg
      421 atgttcgcac ggatttatcc gtaggtcact gttatttata agtttttcta gagcacagcg
      481 tcatttcttg cacgccgcgc aggctttgtg gaatataccg gcgcagggga tatccacaat
      541 attgagtgcg cgttcaatgc cacactcaag cgccatgcaa agaattcctg tgctcgagcg
      601 cggctcgcga ccgccgctac gtgtcggcat cgtcctcgct caccacttca ccttgtccgc
      661 ctttgcggtg ttcgtcgatc atctgcggct ggctgcggac gagggagacc gcagccgacc
      721 cctgcacgtg caatggtcga tcatggcaag ccgtgcagag cccgtgccgg cgagctgcgg
      781 cgtcatgctg cagccgaccc atgggctgct cgctccggag actctcgact acgtggtcgt
      841 gatcggcggg atcctgcatg ccggtccgca ggtcgatgag accaccacgc gctatctgcg
      901 cgaggtgggt actacctcgg taccgctgat cggcatctgc acgggcagtt tcatcctgtg
      961 ccgcgccgga ctcatgaagg gacggcgctc gtgcgtaagc tggtaccact atcaggactt
     1021 cctcgaggcc tttccgaacc aggaggtcat tgccgaccgg ttatttctcg cggatggtcc
     1081 gcgcatcacc tgcgccggcg gcgcgggggc tgcggcgctc gcgacctatc tcatcgagca
     1141 gcatctcggc cgtgcggtcg cacagaaggc gagtcaggtg ctgctgttcg atcgcacgcg
     1201 ctcgggcagc gacgctcagc cgcacccccc gctgtcggag agcgtggccg agccgcgcgt
     1261 gcgccgcgcg ctgctgctga tggagcagaa tctggcacgc ccgatcgcgg ttgcggccgt
     1321 ggccgccgaa ctcgggctct cggcacggca gctcgagcgg ctgtgccgac agcatgttgg
     1381 catgggtccc gcatcgcttt accgtcagct gcgtatgcgt tacgcgaact ggctgatcga
     1441 gaataccgac cgctcggtaa ccgacatcgc caacgaggcg ggcttcgccg actgcgcgca
     1501 tttctcccga cagttcaagg acgcctacgg tctctcaccc accagccgaa gactgcagcc
     1561 ccagcgcgtc gccagcggtg atcatgcgcg tgcacgggtt ttcgagtagt catcacctac
     1621 gtcgcacgca gcaccgcagg caagcggcgg tcgcgggaaa acaacaacag gcaacgggcg
     1681 cgcgcgtacc gccgagctgg cgtgtatatt caatcgcggg cagcgggtgt catattcaat
     1741 cggtaaacca gccacgctgg tgaagctgcg acgcccgggt tttggggagg cacacacatg
     1801 aaatcgaaga tctcatatgc cgttgcggcc attctgggag ggacctccgt cgcagcctcg
     1861 gcgcaggagc aggcagccgg taccaccgag gctaccagcg agggcattgc cgaggtcgta
     1921 gtcaccgcgc agcggcgcag cgagaagatg caggacgtgc ccatctccat gcaggcgctc
     1981 accggtcagg ccctgcagca actcaacatc acgaccttcg acgactatct caagtatctg
     2041 cccaatgtga cctcggccag taacggcccg gggcagaacg aagtcttcat gcggggtctg
     2101 agcgccggct cgcaggcgag ccagggaagc ggctcgacgg gcttgtggcc gaatgtcgcc
     2161 atttacctcg acaaccagtc cggccagctg cccaaccgca acctcgatgt ctacgcggca
     2221 gatatcgacc gcatcgaggt gctcgcgggt ccgcaaggca ccttgttcgg tgccggcgcc
     2281 gaagccggcg tgatccgcta catcaccaac gcacccaagc tcgaccgcac cgaagggagc
     2341 gtgacggcca gctacggcgt gactgccggc ggcgacccga ataccgccgt gcaggcggtg
     2401 ctgaacctgc cgctgatcga gggcaagatg gcggcgcgtg cggtgatcta caacgaccgc
     2461 cgcggcggct acatcgataa tgtgccagcg accttcaccc gcaagaacac cgatatcggc
     2521 atcggttttg cttaccttcc ggctacgggg ggcgtctgcc cggatgggct gccgaacaat
     2581 ggcttctgcg tgccgcccgg aagtccggtg ctcaacaact atctcatcgc gcggaacaac
     2641 atcaacccgg tcagctacca gggtatccgc gcggagctcc tctacaagtt cagcgacgac
     2701 tgggatgtgc tgatcaccca gtcttaccag gacatgcatt cgcagggcgt gttctaccag
     2761 cagcctttcg cctcggacgg cgcgccgctg cagccgctgc aggtcaccct gttcaacacc
     2821 gccgacacca aagacaagtt cgagagcacc gcctggacag tgaacggcaa gttcggcgac
     2881 atacgggccg tgtacaccgg cggctacttg gtgcggaaca tcgatcagac cggtgactac
     2941 acgaactatg cgcgcggcgt gtacgccgac tactatcagt gctacggtcc cggcgcttac
     3001 agcccaaacc tgccgccgaa gtgcttctcc ccgagcgccg gctggcactc ggtcgagcaa
     3061 aacaagcact tccagaacga gctgcgcttc agtacgccgg atgagtggcg gctgcgcggt
     3121 atcctcggcg cgttctggga ggacaaccag ctcttcgacc agaccgcatg gatgtacaag
     3181 gaactcccgc cttgtacctc cagtggcacg gcgggcgtag atccgggcaa tacgggttgc
     3241 ctgtcgaacg tcggctcgat tcccgggacg actgtcgtga accccggtgt gcagaacgat
     3301 cgcacctcct tttaccagga ccaggtacgc gaatataagc agctcgcctt cttcgcctcg
     3361 ctggacttcg atctgatccc gaagaccctg acggtcaccg ccggaacacg ttatttccgc
     3421 ttcgacaact ccatgaaggg cggcgtgctc tcgagtttcg gttgcttcca gcagggattg
     3481 aacggtttca ttgctacggg tgctaccaa
//
LOCUS       DATDMP010000450         3312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1294987, whole genome shotgun sequence.
ACCESSION   DATDMP010000450 DATDMP010000000
VERSION     DATDMP010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294987"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VI195_07875"
     CDS             complement(<1..594)
                     /locus_tag="VI195_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY6824344.1"
                     /translation="MSSHKVVALAYDRLCTFEFGCVVELFALPRPELSVPWYEFAVCC
                     AERAPLRAAGGVEVTVRYALKLLDSADTIVIPGWRDPAETPAAALLARLRRAFERGAR
                     LCSICSGVFVLAAAGVLDGRRATTHWRYAAQLAARYPRILVESNALYVDSGQILTSAG
                     SAAGLDMLLHLVRRDHGARIANQVAQRLVLAPHREGDQ"
     gene            704..1114
                     /locus_tag="VI195_07880"
     CDS             704..1114
                     /locus_tag="VI195_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6824345.1"
                     /translation="MPSVVTQVPAADSASAVQHFERAFSFETDCSDVHEAMRRAPDFV
                     LIDVRSAELFARGHVPRAVNIPHAKIIGSRMAAYAPGTLFVVYCAGPHCNGAERGALR
                     LARLGLPVKIMAGGMTGWMEEGFSLESEAALAAG"
     gene            1189..3027
                     /locus_tag="VI195_07885"
     CDS             1189..3027
                     /locus_tag="VI195_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY6824346.1"
                     /translation="MSKLQKIAAALLVVLLIVAGYGLWATNPAPVRAVKHTRAADAAA
                     AAESALPVIDQNTLLTAQRLARLANTPEEHALAQSAVEIADHELDLAFTGALVHLEAH
                     PPPLSPEAAQIQERLRDAQRTLASDADTVKQLSAQLAQAADADKPAIQDRLDLAQSRM
                     ELAQDEVQEANEDLLQAGGNIHQRIQRMQQEHESAQHNETRSAAAAPASALGSLRGMV
                     GAMRRWLALREKQRSLIEAQRQVMASASELAAERQQMAADLAASKADVPELASRTPHR
                     TSGSAPAAAAPAEHAMTGGSALLGVTRQIAADQHRLTLRDQRLAARRRLADLYGQWQS
                     LVGAEERAVLHGCLQSAAIVLVALLLLLFADRWLEGLLGRTRVDRRQLATLRSVVGVA
                     LQIVAVAVILLMLVGVPGQLGAMLGLVGAGLTVALKDFIVAFVGWFVLMGRNGMRLGD
                     WVEINGVSGEVVELNMFHTVLLETGNWTDAGHPTGRRVTFTNSFAISGHYFNFSTSGQ
                     WLWDELSVMVPYDRDPHAIADAIQKEVTAATAKDASQAEQEWQRASRGQRGVGFTAQP
                     GIAVRPAAGGVEIAVRYVTRASERLALRARLYQSAVQLLSASPVRA"
     gene            complement(2979..>3312)
                     /locus_tag="VI195_07890"
     CDS             complement(2979..>3312)
                     /locus_tag="VI195_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824347.1"
                     /translation="PREWSQRVQSVNYGDDGRVLRSEELRLTNLLDHTPAWLIVPQQL
                     LQFYLQAEFGRGDLAQLKLRKVASFDKPGGRVQLAIYEVTRPSLWAALGAPQARTGEA
                     ESSCTADW"
BASE COUNT          538 a         1142 c         1128 g          504 t
ORIGIN      
        1 ctggtctccc tctcgatgcg gggcgagcac caggcgctgc gcgacctggt tggcgatcct
       61 cgcgccgtgg tcgcgccgca ccaggtgcag gagcatgtcg aggccggccg ccgaacccgc
      121 cgaggtgagt atctgcccgc tatcgacgta cagcgcattg ctctccacga ggatgcgcgg
      181 ataacgcgca gcgagctgcg cagcgtacct ccagtgagtc gtggcgcggc ggccatcgag
      241 cacgccggcg gccgccagta cgaacacccc ggagcagatg gagcacagcc gggcgccgcg
      301 ctcgaatgcg cgccgcagcc tcgccagcag cgctgcggcg ggtgtctccg cggggtcgcg
      361 ccaaccgggg atgacgatgg tgtcggcgct atcgagaagc ttcagcgcgt agcgcaccgt
      421 cacctcgacg ccgcccgcgg cccgcagcgg tgcccgctcc gcgcagcaca ccgcgaactc
      481 ataccacggc acgctcagct ctggccgcgg cagagcgaac aattccacca cgcagccgaa
      541 ctcgaaagtg cacagccggt cgtacgccag cgccactacc ttgtgactgc tcatggcgtg
      601 atattaacgt actatgtcat ttgcgccact gggcgagcca cgcggtccgg ccgcacactc
      661 cactccgcag cactgccgct gcctgcctga aacggagatc agtatgccca gcgtggttac
      721 gcaggtgccg gccgccgaca gcgcgagcgc cgtgcagcat ttcgaacgcg cattcagctt
      781 cgagaccgat tgctcggatg tgcacgaagc catgaggcgc gcccccgatt tcgtgctgat
      841 cgatgtgcgc tctgcagagc tcttcgcccg cggtcatgtc ccccgagcgg tcaacatccc
      901 gcatgcgaag atcatcggct cgcgcatggc cgcctatgcg cccggaacgc tcttcgtcgt
      961 ctactgcgcc ggcccgcact gcaacggcgc cgagcgcggt gccctgcgcc tcgcgcgcct
     1021 gggtctgccc gtgaaaatca tggccggtgg catgaccgga tggatggagg aaggcttcag
     1081 cctcgagtcg gaagcggcgc tagccgccgg ctgagcgcgc gaggagcgcg gtcgcggaag
     1141 cgatggccgt cacggccgcg ctgcagacgg cgtggcatca taggcggcat gagcaagctg
     1201 cagaagatcg ccgcggcgct gctggtggtg ctgctcattg tcgcaggcta cggactgtgg
     1261 gcgaccaatc ccgcgccggt ccgggcggtg aagcacacgc gcgcggccga cgccgcggcg
     1321 gcggccgaga gtgcgctgcc agtcatcgac cagaacacac ttctcactgc gcagcggctc
     1381 gcccggctcg cgaatacgcc ggaagagcac gcgctcgcac agtccgcggt ggagatcgcc
     1441 gatcatgagc tcgatctcgc cttcactggc gcactcgtgc acctcgaggc gcaccctccg
     1501 ccactcagcc ccgaagcggc gcagatccag gagcgactgc gcgacgcgca gcggacactg
     1561 gcgagtgatg cggataccgt caagcagctc agcgctcagc tcgcgcaggc cgcggatgcc
     1621 gacaagcccg ccattcagga ccggctcgat ctcgcgcagt cgcgcatgga gctcgcgcag
     1681 gatgaagtac aggaggcgaa cgaggacctg ctgcaggcgg gaggcaacat ccaccagcgc
     1741 attcagcgca tgcagcagga acacgagagt gctcagcaca acgagacgcg ctccgcggcc
     1801 gcggcgcctg ccagcgcgct cggctccctg cgggggatgg ttggggccat gcgccgctgg
     1861 cttgcgctgc gcgagaagca gcgctcgctc atcgaggcgc aacggcaggt catggcgagc
     1921 gcaagcgagc tggccgccga gcggcagcag atggccgcgg acctggcggc gagcaaggcg
     1981 gatgttcccg agctggccag ccgcacaccg caccggacca gcggcagtgc accggccgca
     2041 gcggcgccgg ccgagcatgc gatgacgggc ggatccgcac ttcttggcgt gactcggcag
     2101 atcgcggccg accagcaccg actgacgctg cgtgatcagc ggctcgctgc gcggcggcgg
     2161 ctcgccgacc tctacggcca atggcagtcg ctggtcggcg ccgaggagcg cgccgtgctg
     2221 cacggctgcc tgcagagtgc ggcgatcgtg ctcgtggcgc tgctgctgct gctgtttgcc
     2281 gaccgctggc tggagggact tctcggacga acgcgtgtcg atcgccgcca gcttgctacg
     2341 ctgcgcagcg tcgtcggcgt tgcgttgcag atcgtcgcgg tggcggtcat cctactgatg
     2401 ctggtgggcg tgcccggcca gctcggtgcg atgctcggcc tggtcggtgc cggcctcacc
     2461 gtggcgctga aggatttcat cgtcgccttc gtcggctggt tcgtgctcat gggacgtaac
     2521 ggcatgcgtc tcggcgattg ggtggagatc aacggcgtga gcggcgaggt cgtcgagctc
     2581 aacatgttcc acaccgtgct gctcgagacc ggcaactgga ccgacgccgg tcacccgacc
     2641 gggcggcgcg tgaccttcac taacagcttc gcgatctccg gacactactt caacttctcg
     2701 acctcgggcc agtggctgtg ggacgagtta tccgtcatgg tgccgtacga tcgcgacccg
     2761 catgccattg ccgatgcgat ccagaaagag gtcaccgcag cgaccgccaa ggatgcgagc
     2821 caggccgagc aggagtggca acgcgcttcg cgcggccagc gcggggtagg cttcaccgcg
     2881 cagccgggca ttgccgtgcg gccggccgcc ggtggagtcg agatcgcggt gcgctacgtc
     2941 acccgcgcaa gcgagcgcct cgcgctgcgc gcgcgcctct accaatcggc ggtacaactg
     3001 ctctccgcct cacccgtgcg cgcctgaggt gcccccaggg ctgcccagag cgagggccgt
     3061 gttacttcgt agatggcgag ttgtacacgc ccccccggct tgtcgaagct ggcgactttc
     3121 cgcagcttca gctgcgcgag gtcgccgcgg ccaaactctg cctgcagata gaactgcagc
     3181 agctgctgcg gcacgatgag ccacgcaggc gtatggtcga ggaggttggt cagacgtagc
     3241 tcctcgctgc gcaatactcg gccatcatcc ccatagttca ccgactgcac gcgctggctc
     3301 cactcccgtg gc
//
LOCUS       DATDMP010000451         4873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_960782, whole genome shotgun sequence.
ACCESSION   DATDMP010000451 DATDMP010000000
VERSION     DATDMP010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960782"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VI195_07895"
     CDS             <1..726
                     /locus_tag="VI195_07895"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY6824348.1"
                     /translation="FAQVLSGWSIGGANARFGAGEPGRFLFRPELHEPGAQVVLGRRY
                     AEDGFGQGAAVLRDLAHQRATARFIASKLARHFISDEPPPQAVARLADVFARSGGDLP
                     SVYAALIDAREAWAEPLAKYKTPADYVVSSFRGLSVPIEADHAALVPFELLGQRTWQP
                     GSPAGWPDRGADWDGASALMKRIQWADAVGARLGNRRDALQLAPQLLGANLSAATRQA
                     IAHAASAAQALTLLLAAPEFMRR"
     gene            741..1958
                     /locus_tag="VI195_07900"
     CDS             741..1958
                     /locus_tag="VI195_07900"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY6824349.1"
                     /translation="MQRREFLRSAAVIAGGTLLCARVSFARPDTRKSRFVLVILRGAL
                     DGLAAVPPLGDRDYAQLRREFALHPPGESGGALPLDGFFGLHPALGFLHECYAARELT
                     VLHACASPYRERSHFDGQDVLENGTPKPHALQSGWLNRALAAVAAPAGREAGVSLGQN
                     VPLVMRGPAAFTSWSPSRLVPPDDDTLSRITDLYARDPLLAARLADALAADAIAAGDD
                     GADTTSAHAPAAARGKARYGEVVRAAGGFLRREEGPKVAVFDTSGWDTHANEGGAQGQ
                     LAARLAELDAGLAALREELGPAWQDTAVLLLTEFGRTAAINGTRGTDHGTATAAFLLG
                     GAVAGGRVIADWPGLSPRALYQGRDLAPTLDVRSVLKGVLAEQLAVPAQVLEQEVFPD
                     SFAARPVRGLLRA"
     gene            complement(2257..2670)
                     /locus_tag="VI195_07905"
     CDS             complement(2257..2670)
                     /locus_tag="VI195_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824350.1"
                     /translation="MSTPTPPISAEAPHVDESLVTYTHVIYALHTLAVLIGITTFHTI
                     VGSFVGGLPSIVAVIMNYARRSATRGTYLESHFRWQIRTFWYALLWAVVCVLVAVTII
                     GIPFALIGFAALAVWIVYRVARGWLALKDGRPMYA"
     gene            complement(2699..3139)
                     /locus_tag="VI195_07910"
     CDS             complement(2699..3139)
                     /locus_tag="VI195_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEY6824351.1"
                     /translation="MSIWRVSPTPEELTARGSRSMPGLLGIHFTEVGPDYLRAAMPVD
                     ERTHQPFGLLHGGASVTLAETVGSVASMLCVDPERYLCVGQEINANHLRGMSSGSVIA
                     TARPFHLGARSHVWHIEIRDERERLVCVSRLTMAIVERRAQQER"
     gene            3204..3905
                     /locus_tag="VI195_07915"
     CDS             3204..3905
                     /locus_tag="VI195_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY6824352.1"
                     /translation="MSAFVFPPPAQPSVAVAGSDARFPVHRIYCIGLNYADHVREFGR
                     DPERAPPVFFMKPADAVVANGAAVPYPPGTANFHYEIELVVALARGGRDVAAQDARGL
                     VFGYAAGNDLTRRDLQSAAKERGEPWDTSKGFDGSAPLAAIRPVSAGHVTAGRIWLSV
                     NGSVRQESDISRMLWDVPQIIAALSRLYELRAGDLIFTGTPAGVGAIRPGDTLEGGIE
                     GLEVLRNSIVAGARV"
     gene            3909..4559
                     /gene="maiA"
                     /locus_tag="VI195_07920"
     CDS             3909..4559
                     /gene="maiA"
                     /locus_tag="VI195_07920"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026437509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY6824353.1"
                     /translation="MKLYGYFRSSATYRVRIALGLKGIAWETVVVDLRAPARAQHRPE
                     FRALNPHGLIPVLLDGEHTLTQSLAIIEYLDETHPHPALLPRAPSERAEVRALALAVA
                     CDIHPLNNSRVLEYLRRQLAQDEAAVNAWYAHWVAQGFAALERDAQRLSGDGRHMYGT
                     SVSLADVCIVPQMANARRFNCDLAPYPTLRAISAHLESLPAFAQAAPGAQPDASPA"
     gene            4569..>4873
                     /locus_tag="VI195_07925"
     CDS             4569..>4873
                     /locus_tag="VI195_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY6824354.1"
                     /translation="MSRRSFLRSSAALLTAAGADWLLTGAAPAPRGPHEEEPAPFDFA
                     RLKGLARTRGAQAYVAPAKRLPPAIAALDWDHWQAIRFRDERSLWADEGLRFQVRFA"
BASE COUNT          715 a         1669 c         1719 g          770 t
ORIGIN      
        1 ttcgcgcaag tgctcagcgg ctggtcgatc ggtggcgcga acgcgcgctt tggagctgga
       61 gagcccggcc gcttcctgtt ccgtccggag ctgcacgagc cgggcgcgca agtcgtgctc
      121 gggcggcgtt acgccgagga cggcttcggg cagggtgcgg cggtactgcg cgatctcgcg
      181 caccagcgcg ccacggcgcg cttcatcgcc agcaagctcg cccgccactt catcagcgat
      241 gagccgccgc cgcaggcggt ggcgcggctc gccgatgttt ttgcgcgcag cggcggagac
      301 ctgccgagtg tctacgccgc gttgatcgat gcgcgcgaag cctgggccga gccgctcgcc
      361 aagtacaaga ctcccgcgga ctacgtggtg tcgagcttcc gcggcctttc ggtacccatc
      421 gaagcggatc atgcagcgct cgtgccgttc gagcttctcg ggcagcgcac ctggcagccg
      481 ggatcaccgg cggggtggcc ggaccgcggc gccgactggg atggagcgtc ggcattgatg
      541 aagcgtattc aatgggccga cgcggtgggc gcgcggctcg gcaaccgccg cgatgcgctg
      601 cagctcgccc cgcagttgct cggcgcgaat ctcagcgcag cgacgcgcca ggccatcgca
      661 catgccgcaa gcgcagcgca ggcgctcacc ctgctgctcg ccgcgcccga gttcatgcgg
      721 cgctgaacgg agagaccagg atgcaacggc gtgaattcct gaggagcgca gcagtcatcg
      781 cgggcggcac gctgctgtgc gcgcgggtgt catttgctcg acccgacacg cgcaaatcgc
      841 gcttcgtgct ggtgatcctg cgcggcgcgc tcgatggact cgcagccgtg ccaccgctcg
      901 gtgaccgtga ctacgcgcag ctgcggcgcg agttcgcgct gcatccaccg ggggagagcg
      961 gcggcgcgct gccgctcgat ggtttcttcg gcttgcatcc cgcgctgggt ttcctgcacg
     1021 agtgctacgc ggcgcgcgag ctcacggtgc tgcatgcgtg tgccagcccc taccgcgaga
     1081 gatcacactt cgatggacag gacgtgctcg agaatggcac cccgaagccg cacgcgctgc
     1141 agagcggttg gctcaaccgc gcgctcgccg cggttgccgc cccggccggg cgcgaggcgg
     1201 gcgtatcgct cggacagaac gtgccgctgg tgatgcgtgg ccccgcggcg ttcacttcct
     1261 ggtcgccctc gaggctcgtc ccgcccgacg atgacacctt gtcgcgcatc accgatctct
     1321 acgcgcgcga tccgctgctt gcggcgcgcc tggcggacgc actggccgcc gatgcgatcg
     1381 cggcaggcga tgatggagcg gacaccacga gcgcgcacgc ccccgcggct gcgcgcggca
     1441 aggcccgcta cggcgaggtg gtgcgcgcgg ccgggggatt cctgcgccgt gaggagggcc
     1501 cgaaggtcgc cgtgttcgat accagtggct gggatacgca cgccaacgag ggtggggcgc
     1561 aggggcagct ggccgcgcgt ctcgctgagc tcgatgccgg gctcgcggca ctgcgggagg
     1621 agctcggccc cgcgtggcag gacaccgcgg tgctgctgct caccgagttt ggccggacgg
     1681 cggccatcaa cggcacgcgc ggcaccgacc acggcaccgc aaccgcggct ttcctgctcg
     1741 gcggtgcggt cgccggcggc cgcgtgatcg ccgactggcc cgggctgtcg ccgcgagcgc
     1801 tctatcaggg gcgcgacctt gcgcccacgc tcgatgtgcg ctcggtgctc aagggagtgc
     1861 tggcggagca gcttgccgta cccgcgcagg tgctggagca ggaagtgttt cccgacagct
     1921 tcgccgcaag accagtgcgt ggattgctgc gcgcctgaga cccgagaccg ctgcgcaact
     1981 acttcgggtt ctgatcgtcg tccgggccgg atttcttcgg cggcgcaggg gtacccgatg
     2041 gcggcggcgg ttccgggcta ctctgcggct gcggagcagg cgatgtcgca ggcggctgcc
     2101 cggagggcac cggcgcgggc ggaggcgcct cggccggtgc cgtaaccacg gctccgcctt
     2161 tctcgggcag gtatagagga cctttctggc cgcagcccgc gagcagcaac gcggcagagg
     2221 ccgcgagcca agccccgcga gcgccggctg ggagcctcat gcgtacatcg ggcgcccgtc
     2281 cttgagcgcg agccacccac gagccacgcg gtagacgatc cacacggcga gcgcagcgaa
     2341 gccaatgagc gcgaacggaa tgccgatgat tgtgaccgcc accagcacgc agaccaccgc
     2401 ccacagcaac gcgtaccaga aggtgcggat ctgccagcgg aagtgggatt cgaggtaggt
     2461 gccgcgggtc gccgagcggc gcgcgtagtt catgatcacc gcgacgatcg atggcagtcc
     2521 gccgacgaag ctgccgacga tggtgtgaaa ggtggtaatg ccgatcagaa ccgcgagcgt
     2581 atgcagcgcg tagatgacat gcgtgtaggt caccagtgac tcatcgacat ggggcgcttc
     2641 ggcgctgatc gggggagtcg gggtcgacat gttgtacctc cgcttactgc gctcctggct
     2701 acctttcctg ctgtgcccgg cgctcgacga tcgccatggt gagccgcgag acacacacca
     2761 gccgctcacg ctcgtcgcgg atctcgatgt gccacacgtg actgcgggcg ccgagatgaa
     2821 aggggcgcgc ggtcgcaatg acgctccccg aggacatccc gcgcagatgg ttggcgttga
     2881 tctcctggcc gacgcaaagg tagcgctcgg gatcgacgca tagcatggaa gcgacgctgc
     2941 cgaccgtctc ggcgagggtc accgaagcgc cgccatgcag gagtccgaag ggctgatggg
     3001 tgcgctcgtc gaccggcatg gccgcgcgca ggtagtcggg ccctacttcc gtgaagtgga
     3061 tgccgagcaa gcccggcatg ctgcggctgc cccgggcggt gagctcctcg ggtgttgggc
     3121 tgacgcgcca gatgctcatc gcggctagtc tagcggtttg cgcaatggca atctgcctta
     3181 cccaggatcc gcaggcgccc atcatgtccg cattcgtctt tcctccaccg gctcagccaa
     3241 gcgttgccgt agcgggtagc gatgcccgtt tcccggtgca ccgcatttat tgcatcggcc
     3301 tcaactatgc cgaccacgtg cgcgagttcg gtcgcgaccc cgagcgtgct ccgccggtct
     3361 ttttcatgaa gccggccgat gcggtggtgg cgaatggtgc cgcggtgccc tatcccccgg
     3421 gtaccgccaa cttccactac gagatcgagc tggtggtggc gctcgcgcgc ggcggccgcg
     3481 acgtcgctgc gcaggatgcg cgcggcctgg tgtttggcta cgccgcgggc aacgacctta
     3541 cacgccgcga cctgcagagc gccgccaagg agcgcggcga gccctgggat acctccaaag
     3601 gcttcgacgg ctcggcgccg cttgccgcca tccgtccggt gagcgccgga cacgtgaccg
     3661 ccgggcgcat ctggctcagc gtgaacggca gcgtgcgcca ggagtcggac atcagccgca
     3721 tgttatggga cgtaccgcag atcatcgcgg cgctctcccg gctctacgag ttgcgcgccg
     3781 gcgatctcat ttttaccggc accccggcgg gcgtcggtgc gatcaggccc ggcgacacgc
     3841 tcgaaggtgg gatcgagggg ctcgaagtcc tgcgtaacag catcgtggcc ggggcgcgcg
     3901 tttaagccgt gaagctctac ggctacttcc gcagctcggc gacctaccgc gttcgcatcg
     3961 cgctcgggct gaaaggcatc gcctgggaaa cggtggtcgt cgacctgcgc gcgcccgcgc
     4021 gcgcgcagca caggcccgaa ttccgcgccc tcaatccgca cgggctcatc ccggtgctgc
     4081 tcgatggcga gcatacgctc acgcaatcgc tcgcgatcat cgagtacctg gacgagacgc
     4141 atccgcatcc cgcgctgctg ccgcgcgcgc cgtcagagcg cgccgaggtg cgcgcgctcg
     4201 ccctggcagt ggcctgcgac atccacccgc tcaacaattc gcgcgtgctc gagtatctgc
     4261 gccggcagct cgctcaggat gaggcggcgg tgaacgcgtg gtacgcgcac tgggtcgccc
     4321 aggggtttgc agcgctcgag cgcgatgcgc agcgcctgag cggtgacggg cggcacatgt
     4381 acggcacgag tgtttcgctg gccgatgtct gtatcgtgcc gcagatggcc aacgcccgcc
     4441 gcttcaactg cgatctcgcg ccctatccca cgctgcgcgc gatcagcgcg cacctcgagt
     4501 ccctgccggc attcgcgcag gctgcccccg gggcgcagcc ggacgcttca ccggcgtagc
     4561 agggacgcat gtcgcgcaga agctttctcc gctcgagtgc cgcgttgctc accgctgccg
     4621 gcgccgattg gctgctgacg ggcgccgccc ctgcgccgcg cggtccgcac gaggaagagc
     4681 cggcgccctt cgactttgcg cggctcaaag gcctcgcgcg gacgcgcggc gcgcaagcct
     4741 acgtcgcgcc ggccaagcgc ctgccgcccg cgattgcggc gctcgattgg gaccactggc
     4801 aggcgatccg ctttcgcgac gagcgctcac tgtgggcgga tgagggcttg cgcttccagg
     4861 tgcgcttcgc cca
//
LOCUS       DATDMP010000452         2603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_850370, whole genome shotgun sequence.
ACCESSION   DATDMP010000452 DATDMP010000000
VERSION     DATDMP010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850370"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VI195_07930"
     CDS             <1..1750
                     /locus_tag="VI195_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY6824355.1"
                     /translation="LSFSDSHAAAVLQALGRPAPPKPAFDVDLAQRWTFSKDPQNLGL
                     SQHWYAGGHQPASWRPIRTDLPWEQQGYAEYHGIGWYRASVQLPAKVPTPLYLYLGEV
                     SSRYRVFVNGREVTHSYDHWPRRLTYADIAPYVSAGGPNEIVLRVEGDTYGSGILRGP
                     VALRDIAPPERITFDLADEKQADIFMRYLHGPLMDEGVDLWWVDGGSGATGMAGLNAQ
                     LWTNKVFYDFSQRHTGKRTFILARYGEWGSERYPGFFTGDAYSEWPVLAYEVAFSARG
                     GNVLVPYISHDIGGFHGGKIDFELYARWIEFGTFSAILRMHSAHENPRDANPRMPWVY
                     GERGVELMRKYFTLRTQLIPYLYTYSWLAHRDSLPLLRPLYLEYPELEEAYRHPYEYF
                     LGAQLLVAPVVTPGNEQQLYLPPGEWRDFFTGKRYRGGAMLTAHYPVDATPLFVREGA
                     VVPEQRASEDSNAKPLDHLVLTVYGSGQGSFELYEDDGISLDYEARHAHTAITHSSGN
                     DGANRLLIEPAQGEYPGQPVARSYELRLYTDARPTMIAIDGKDAGPWHWDGKRSLAVV
                     GLPDRSVHQRVRVEWH"
     gene            1754..>2603
                     /locus_tag="VI195_07935"
     CDS             1754..>2603
                     /locus_tag="VI195_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY6824356.1"
                     /translation="MSERSARGQGSYTRRSFLRAAGASGAAVIAPQLFPPARAQPPAR
                     SKPNLVFFLGEGARWDESSLAGNTLLKTPNIDRIGREGAEFTEAFCINSLCLPARATI
                     LSGLYSHTTGAVDNQHSKVPERFPIVTDLVRAQGYEVAFIGKSHVQGALMDHYWDYYF
                     GFAGQADYQCPVVIEGTRGQFGPPRSYEEYVDDLLTRKAVEWLRQKRSMPFCLFLWFY
                     APHAPFDRPLRMVNDFNGVAIPKPAGFDEYLRGYAGKPRGVIEARNKIGSQFLQRDDP
                     RSLEELV"
BASE COUNT          455 a          849 c          849 g          450 t
ORIGIN      
        1 cctgtcattc agcgatagtc atgccgcggc ggtgctgcag gcactcggcc gaccagcacc
       61 cccgaaaccg gcattcgatg tggacctcgc gcagcggtgg acgtttagca aggatccgca
      121 gaatctcggc ctcagccagc actggtatgc gggtggtcac cagccggcga gctggcgccc
      181 gatccgcacg gacctgccat gggagcagca gggatacgcc gagtatcacg gcatcggctg
      241 gtatcgcgca tcggtccagc tgcccgcaaa ggtgccgaca ccgttgtacc tgtacctcgg
      301 cgaagtcagc agccgctacc gggttttcgt caacggccgc gaggtgacgc acagctatga
      361 tcattggccg cggcgtctta cctacgccga catcgctcct tatgtctccg ccggaggccc
      421 gaacgagatc gtgctgcggg tcgagggcga tacctatggc agcggcatcc tgcgcggacc
      481 ggtggcgctg cgcgacatag cgccgcccga gcggatcact ttcgacctcg cagatgagaa
      541 gcaggccgac atcttcatgc gctatctcca cgggccgctc atggacgagg gtgtggatct
      601 ctggtgggtc gatggcggga gcggcgccac cggcatggcg ggtctcaacg cccagctgtg
      661 gaccaacaag gtcttctacg acttcagtca gcggcatacc ggcaagcgca ccttcatact
      721 cgcgcgctac ggagagtggg gaagcgagcg ctatccgggt ttcttcaccg gggatgccta
      781 ctcggagtgg ccggtgctcg cctatgaggt ggccttctcg gcacgtggtg gcaacgtgct
      841 ggtgccgtac atcagccacg acatcggcgg cttccatggg ggcaagatcg atttcgagct
      901 ctacgcgcgc tggatcgagt tcggcacgtt cagcgccatt ctgcgcatgc atagcgctca
      961 tgagaacccg cgcgacgcca acccgcgcat gccctgggtc tacggcgagc gcggtgtcga
     1021 gctgatgagg aaatacttca ctctgcgcac gcagctcatc ccctacctct acacctacag
     1081 ctggctggcg caccgcgact ccctgccgct gctgcggccg ctgtatctcg agtatccgga
     1141 gctcgaggaa gcctaccgcc atccctacga atacttcctc ggcgcgcaac tgctggtggc
     1201 gccggtggtg acaccgggca atgagcagca gctctatctg ccccccggcg agtggcgcga
     1261 cttctttacc ggcaagcgct accgtggtgg tgcgatgctg acggcgcact acccggtcga
     1321 tgcgacaccg ctgttcgtgc gcgaaggcgc ggtcgttccc gaacagcgcg ccagcgagga
     1381 ctcgaatgcc aagccgctcg atcatctggt gctgaccgtc tacggctcgg gccagggaag
     1441 cttcgagctc tatgaggacg acggcatctc gctcgactac gaagcgcggc acgcgcacac
     1501 cgcgatcacc cacagcagcg gcaatgatgg ggcgaatcgt ctcctcatcg agccggcgca
     1561 gggcgagtat ccggggcagc cagtcgcgcg ctcgtacgag ctgcggctgt acaccgatgc
     1621 gaggcccacg atgattgcga tcgacggcaa ggatgcaggg ccctggcact gggatggcaa
     1681 gcggtccttg gcggtggtgg ggctcccgga tcgttcggtc caccagcgcg tgcgcgtaga
     1741 gtggcactag cacgtgagcg agcggtcagc cagaggccag ggcagctaca cgcgccgcag
     1801 ctttctgcga gctgccggcg caagtggcgc ggccgtcatc gctccgcagc tctttccgcc
     1861 cgcccgcgcc cagccgccag cgcgcagcaa gcccaacctg gtgttctttc tgggagaggg
     1921 ggcgcgctgg gatgagtcct cgctcgccgg caacacactg ctgaagactc ccaacatcga
     1981 tcgcatcgga cgcgaaggcg ccgagttcac cgaggccttc tgcatcaact cgctgtgcct
     2041 gccggcgcgc gccacgattt tgagcggact ctattcgcac accaccggcg cggtcgacaa
     2101 ccagcacagc aaggtgccgg agcggtttcc gatcgtcacc gatctggtcc gcgcccaggg
     2161 ctatgaggtg gcattcatcg gcaagtccca cgtgcagggc gcgctgatgg atcactactg
     2221 ggactactac ttcggattcg cggggcaggc cgactaccag tgcccggtgg tcatcgaggg
     2281 gacccgcgga cagttcgggc cgccgcgcag ctacgaggag tacgtcgatg acctgctcac
     2341 gcgcaaggcg gtcgagtggc taaggcagaa gcgctcgatg ccgttttgtc tgttcttgtg
     2401 gttctacgcc ccgcacgcgc cgttcgatcg gccgctgaga atggtgaacg acttcaacgg
     2461 cgtcgccatt ccgaagccgg cgggcttcga tgagtacctg cgcggttatg cgggcaaacc
     2521 ccgcggcgtg atcgaggcac gcaacaagat cggcagccag ttcctgcagc gcgacgaccc
     2581 gcgctcgctc gaggagttgg tga
//
LOCUS       DATDMP010000453         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_628411, whole genome shotgun sequence.
ACCESSION   DATDMP010000453 DATDMP010000000
VERSION     DATDMP010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628411"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1154)
                     /locus_tag="VI195_07940"
     CDS             complement(183..1154)
                     /locus_tag="VI195_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6824357.1"
                     /translation="MLDSEPAVPHGAPAEPLDAYSAAVVGALEQVAPAVVSVYVGGAL
                     EAERARGGAGSGVVVTPDGYLLTNEHVVQQVNEARVTFVDGRTAPAVVAGRDPSTDLA
                     VLRAQAAALPYARLVPEQRLRPGQLVIAVGNPLGFESTVSAGVVSALGRALRSRHGRL
                     IESVVQHTAALNPGNSGGPLVDSLGRVAGINTAIIAMAQGIGFAIPASTAQWVLTEIL
                     TQGRVRRAWLGIAARDRPLDLRLVRALGLDARRAVDVLSRESAGPAATSDLRPGDLIV
                     AVNREAVDGIDALHRLLSRVPPGSELTLQVVRRTQLLEVRLTVREPP"
     gene            1180..>2321
                     /locus_tag="VI195_07945"
     CDS             1180..>2321
                     /locus_tag="VI195_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY6824358.1"
                     /translation="MKHKGPGRADSCENHATIRMISLTQTAILLAAAVIAVSLFRFAR
                     LSSILGYLAAGLVIGPWGLNLIGGIGHITQVAEFGIVLLLFIIGLELQPTRLWVMRRT
                     VFGLGTAQVVVCTLLLGTIAWALGQALATAAVIGFGLSLSSTPLVLQVLAERRELKSQ
                     HGRAAFGILLFQDLAVLPALAILPLLEPLPPSHPSAGPWWLSLLKLLAVGAAVLIGGG
                     RIALRPALRLVARVQVSEVFTAAALLTVILTALFANRLGLSSALGAFLAGVLLADSEF
                     RHELEADLEPFKGLLLGLFFISVGMAANLGLLRSELLTLLAVTVGFLTVKVLAITALG
                     KLAGLRGDSPLRLGFALPTGGEFAFVLFTLAARGKLIDPATVDLLVL"
BASE COUNT          338 a          826 c          771 g          386 t
ORIGIN      
        1 gggcttgcga gctcgcttcg tggaccgact gtcttacacc atcactatgc gtccgataat
       61 accggcccgc gaagctcttg caagcaccgc tccgcatgag accccggggc cgatacttca
      121 ccgatcggtt aaccattgat gagagcactc tccagagcgc tcctaccgcg ggcgggcgag
      181 actcagggcg gctcgcgcac ggtgagccgc acctcaagaa gctgcgtgcg ccgtaccact
      241 tgcagcgtca gctcgctccc cgggggcacg cggctcagca agcggtgcaa cgcatcgatc
      301 ccgtccacgg cctcgcggtt caccgccacg atgagatccc ccggccgcag gtctgaggtc
      361 gcggcgggtc ccgcgctctc ccgcgagagc acgtcgaccg cacggcgcgc gtccagcccg
      421 agcgcacgca ccaggcgcag atcgagcgga cggtcgcgcg cagcgatgcc gagccaggcg
      481 cggcgcaccc ggccctgcgt caggatctcc gtcaacaccc actgtgcggt cgacgccgga
      541 atggcaaagc cgattccctg cgccatggca atgatcgcgg tgttgatgcc ggctacgcgt
      601 ccgagcgaat cgaccagcgg gccgccggag ttaccggggt tgagcgcggc ggtgtgctgc
      661 accacgctct caatgagccg tccgtggcgg ctgcgcagcg cgcgtccgag tgcgctcacg
      721 actcctgccg acaccgtgga ttcgaaaccg aggggattgc cgaccgcgat gacgagttga
      781 ccgggccgca gacgctgctc tggcacgagc cgtgcgtaag gcagcgccgc cgcctgggca
      841 cgcagtaccg ccagatcggt cgagggatca cggccggcga ccacggccgg agccgtgcgg
      901 ccatcgacga aggtgacgcg cgcctcattc acctgctgga cgacgtgctc gttggtgagc
      961 aggtagccat cgggcgtcac cacgacaccc gagccggccc cgccgcgagc gcgctcggcc
     1021 tcgagcgcac ccccgacata gacgctgacc accgccggcg ccacctgctc gagcgctccg
     1081 accacagccg ccgagtaggc atcgagcggc tccgcgggtg cgccgtgcgg gacggccggt
     1141 tcactgtcga gcatgcgtgc tgggatgggg accgccggca tgaaacacaa gggccccggc
     1201 cgcgccgatt cctgcgagaa tcatgcgaca atccgcatga tcagcctcac gcaaaccgcc
     1261 attctgctgg cagcggccgt catcgccgtg tcgctgtttc gcttcgcgag actgtcctcg
     1321 atcctcggct atctcgccgc cgggctcgtg atcgggccct gggggctgaa cctcatcggt
     1381 ggcatcgggc acatcacgca ggtcgccgag ttcgggatcg tgctgctgct cttcatcatc
     1441 gggctcgagc tgcagccgac gcggctatgg gtgatgcgcc ggaccgtctt cggcctcggc
     1501 accgcgcagg tggtggtgtg cacgctgctg ctcgggacga tcgcctgggc gctcgggcaa
     1561 gcgctggcga ccgcggccgt catcggcttc ggtctgtcgc tctcctcgac gccgctcgtg
     1621 cttcaggtac tcgccgagcg gcgcgagctg aagagtcagc acggacgtgc cgccttcggc
     1681 atcctgctgt tccaggacct cgcggtactg cccgcgctcg cgatcctgcc actgctcgag
     1741 ccactcccac cgagtcatcc aagtgcgggg ccctggtggc tgagcctgct caagctcctc
     1801 gcggtcggcg cagccgtgct gatcggcggc ggccgcatcg cgctgcgtcc cgccctgcgg
     1861 ctcgttgcgc gcgtgcaggt gagcgaggta ttcacggctg cggcgctgct caccgtcatt
     1921 ctgacggcac tgttcgccaa ccgcctggga ctctcgagcg cactcggcgc cttcctcgcc
     1981 ggggtgctgc tcgcggacag cgagttccgg cacgagctcg aggcagatct cgagccgttc
     2041 aagggactgt tgctcggcct gttcttcata tccgtcggca tggcggcgaa cctgggattg
     2101 ctgcgctcgg agctcctgac gctgctcgcc gtcacggtgg gctttctcac ggtaaaagtc
     2161 ctcgccatca ccgcgctagg caagctcgca gggctgcgcg gggactcgcc gctgcggttg
     2221 ggattcgcgc tgccgaccgg aggggagttt gccttcgtgc tatttaccct cgccgcccgt
     2281 gggaagctca tcgacccggc caccgtggac ctgctcgttc t
//
LOCUS       DATDMP010000454         2059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_516841, whole genome shotgun sequence.
ACCESSION   DATDMP010000454 DATDMP010000000
VERSION     DATDMP010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516841"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VI195_07950"
     CDS             <1..496
                     /locus_tag="VI195_07950"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY6824359.1"
                     /translation="VAMAQNGVIGRDNTLPWRLPEDLKRFKSFTLGKPILMGRKTFES
                     IGRPLPERTNFVLTRDRGWSAAGVTVAHSVEEALARAAGSEELVAIGGAEVYRLVLPF
                     ARRIYLTHVHADVPGDTYFPEFDATQWADVECRMHPADERHAYPVTFVTLERRNAPEV
                     PRQH"
     gene            complement(515..1876)
                     /locus_tag="VI195_07955"
     CDS             complement(515..1876)
                     /locus_tag="VI195_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6824360.1"
                     /translation="MRLPAPARVNIIGAGLAGALLALLLARRGIAVALYERRPDPRQA
                     RPERGRSINLALAARGMRALERAAVLGRVEPLLIPMRGRMIHDRGGAPVLQPYGQREH
                     EVIFSVGRADLNRVLIETAARHANVSVHFNQQCVGADVRRHHLLLRDTAEGTEYQLPL
                     APTVATDGAGSAVRASLATAGLLEVREDWLDHDYKELTIPAGAAGALERHALHIWPRG
                     GFMLIALPNTDGSFTATLFLARSGSSSFAALDSAEAVQQFFAREFADALPLLPDLAAQ
                     FAAHPQGQLGTVHAAPWHLAGEVLLLGDAAHAIVPFHGQGMNAAFEDCAELDALLEAA
                     GSWSELFARFEQSRRENSTAIAQMALENYLEMRDTVLDAGFVRRKALAMALERRFPER
                     FIPRYSMVMFHPEISYAEARRRGALQEQLLQELDAAAVGAESALAERLVHARLAALRA
                     ASL"
     gene            complement(1873..>2059)
                     /locus_tag="VI195_07960"
     CDS             complement(1873..>2059)
                     /locus_tag="VI195_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824361.1"
                     /translation="LRLAAGEHRGKQVFDALTARGVVCDWRSPDIIRVAPVPLYNRFE
                     DLWRFARALGDILQAAA"
BASE COUNT          325 a          715 c          729 g          290 t
ORIGIN      
        1 cgtcgccatg gcgcagaacg gtgtcatcgg acgcgacaac acgctcccct ggcgcctgcc
       61 ggaggatctc aagcgcttca agtccttcac gctgggcaaa ccgatcctca tggggcgcaa
      121 gaccttcgag tccatcgggc gcccgctgcc ggagcgcacc aacttcgtgc tgacccgcga
      181 ccgaggctgg tccgccgcgg gcgtgacggt cgcacattcg gtggaggagg cactggcccg
      241 cgccgccggc agtgaggaac tggtggcgat cggcggcgcg gaggtttacc gcctggtgct
      301 gccgttcgcg cgccgcatct atctgacgca cgtgcacgcg gacgtcccgg gcgacacgta
      361 ctttccggag ttcgacgcca cgcagtgggc ggatgtcgaa tgccgtatgc atccggcgga
      421 tgagcgccat gcctacccgg tcacctttgt gacgcttgag cggcgcaacg cccccgaggt
      481 gccgcgacag cattgacgcg gccggcgagc ctcactacag cgacgccgcc cggagcgcgg
      541 caagccgtgc atggaccagc cgctcggcga gggccgactc cgctcccacg gccgcggcgt
      601 ccagctcctg cagcagctgc tcctgcagcg cgccgcgccg ccgggcctcg gcataggaaa
      661 tttccgggtg gaacatgacc atcgaatagc gcggtatgaa acgctcgggg aagcgccgct
      721 cgagcgccat ggcgagcgcc ttgcggcgca cgaagccggc atcgagcacg gtgtcgcgca
      781 tctccaggta gttctccagc gccatctgtg cgatggccgt gctgttctcg cggcgcgact
      841 gctcgaagcg tgcgaacagc tccgaccagc ttccggcagc ctcgagcagg gcatcgagct
      901 cggcgcagtc ctcgaatgcc gcattcatcc cctgcccgtg gaagggcacg atggcatgcg
      961 cggcatcgcc gagcagcagc acctcgcccg cgaggtgcca gggtgcggcg tgcaccgtgc
     1021 cgagctgccc ctgcgggtga gcggcgaact gggccgcgag atccggcagc agcggcagag
     1081 catcggcgaa ctcccgcgcg aagaactgct gcaccgcctc ggccgagtcg agcgcggcga
     1141 agctcgatga gccgctgcgc gcgaggaaca gcgtcgcggt gaagctgccg tcggtattcg
     1201 gcagcgcgat cagcatgaag ccgccgcgtg gccagatatg cagcgcgtgg cgctcgagtg
     1261 cgccggccgc gcccgccggt atggtgagct ccttgtagtc gtgatcgagc cagtcctcgc
     1321 gcacctcgag cagccctgcg gtggcgaggc tggcgcgtac ggccgagccg gcaccatcgg
     1381 ttgcgacggt gggggcgagc ggcagctggt actcggtgcc ctccgcggta tcgcgcagca
     1441 gcagatgatg gcgacgcacg tccgcaccga cgcactgttg gttgaagtgc acactgacgt
     1501 tcgcgtgccg agcggccgtc tcgatcagca cacggttgag atccgcgcgg cccaccgaaa
     1561 atatgacctc gtgctcgcgc tggccgtagg gctgcagtac gggcgcgccg ccgcgatcgt
     1621 ggatcatgcg tccgcgcatc gggatcagca gcggctcgac acgcccgagc acggcggcgc
     1681 gctcgagcgc ccgcatgccg cgggcggcga gcgcgagatt gatggagcgg ccacgctcgg
     1741 gccgtgcctg ccgcgggtca gggcgccgct cgtagagtgc gactgcgatt ccgcgccgcg
     1801 ccagcagcag cgcgagcagc gcgcccgcaa gtccggcgcc gatgatattg acccgagccg
     1861 gcgcaggcag cctcatgccg ctgcctgcag tatgtcgccg agcgcgcgtg cgaagcgcca
     1921 gaggtcctcg aagcggttat agagcggcac cggcgccacc cgaatgatgt cgggcgagcg
     1981 ccagtcgcac accacgcccc gggcagtgag cgcatcgaac acctgcttgc cgcggtgctc
     2041 gccggctgca aggcgcagc
//
LOCUS       DATDMP010000455         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_961502, whole genome shotgun sequence.
ACCESSION   DATDMP010000455 DATDMP010000000
VERSION     DATDMP010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961502"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VI195_07965"
     CDS             complement(<1..540)
                     /locus_tag="VI195_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY6824362.1"
                     /translation="MRKNAQELFQLARAALEKAGAHSKMAEAAAHHLVQAEAQGLPTH
                     GMSRVPFYCGMLRNGRADGAAHPALVAERGGACLIDNRDGLPYGSVQWALEELIQRTR
                     RNGIGYAGIRNSAHVGVLGIHLAPLAEAGLVGFAFTNSPAAIPPWGGKKALFGTNPVA
                     GIFPRAGKEPIVVDLALTTV"
     gene            580..1269
                     /locus_tag="VI195_07970"
     CDS             580..1269
                     /locus_tag="VI195_07970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6824363.1"
                     /translation="MGKMIELTAADGHKLAAYRADPAGTGATAGKPRGGIVVIQEIFG
                     VNSHIKSVADGFAKDGYVAVAPAMFDRTQRGYDTGYSQPEIQAGVAIMQKLDWKQTLL
                     DVDAAVAEAKKAGKVGIVGYCWGGTVAWLAAAKTAGLACAVPYYGGGMPNFINEKPKI
                     PVLCHFGEQDQSPTLEQAKAIAKAHPGITAHFYPGAGHGFNCDQRPSFNAAASKQARE
                     RTLEFFRKHVG"
     gene            complement(1270..1860)
                     /locus_tag="VI195_07975"
     CDS             complement(1270..1860)
                     /locus_tag="VI195_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6824364.1"
                     /translation="MKPALHRRDVVKSLLSGRKNLLVVAGLGSTAWDITAAGDHELNF
                     PLWGAMGQAAMMGLGLALAQPKRRVLVITGDGEMLMGLGSLATIGVQQPKNLSIVVID
                     NGRYGETGMQATHTASGVDLAAVARACRFASCSLVTEKAALAGLRQLIHGKPGPHFAA
                     IKVVAEKIPLVLPPREGALLKARFRRALLGPKADLV"
     gene            complement(1857..>2228)
                     /locus_tag="VI195_07980"
     CDS             complement(1857..>2228)
                     /locus_tag="VI195_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824365.1"
                     /translation="GIGLAIGAWLGGEKSVLLMQSSGVGNVINVLGTVKECRFPLVSL
                     VTMRGEQGEFNPWQVPMGQATRQVLEAMGTIVHYPAGAQDIGPTVNAALRLAYNSYAS
                     VAVLIPQRVIGIKSFQEQASR"
BASE COUNT          368 a          763 c          748 g          349 t
ORIGIN      
        1 tacggtcgtg agcgcaaggt ccaccacgat cggctccttg cccgcgcgcg ggaagatgcc
       61 ggcgacggga ttcgtgccga agagcgcctt cttgccgccc caaggcggaa tggccgccgg
      121 cgaattggta aaggcgaagc ccacgagccc ggcttcggcg agcggcgcca ggtggatgcc
      181 gagcaccccg acgtgcgcgc tgttgcggat gccggcatag ccgatgccgt tgcggcgcgt
      241 gcgctggatg agctcttcga gcgcccattg cacggagccg tagggcaggc catcgcggtt
      301 gtcgatgagg caggcgcccc cgcgctccgc gaccagggcc gggtgcgccg ccccgtccgc
      361 gcgcccgttg cgcagcatgc cgcaatagaa cggcacgcgc gacatcccat gggtgggcaa
      421 gccctgcgcc tccgcctgca ccaggtggtg cgccgcagcc tcggccatct tagaatgcgc
      481 ccctgccttc tcgagggccg cacgcgccag ttgaaatagc tcttgcgcgt tcttccgcat
      541 cgaggggatt ctaattaatt ctggcgagga gattgccgca tgggcaagat gatcgaactc
      601 actgcagccg acggacacaa gcttgcggcc tatcgcgccg atccggctgg gacgggtgcc
      661 acggcaggca agccgcgcgg cggcatcgtc gtgatccagg agatcttcgg ggtgaacagc
      721 cacatcaagt cggtcgccga cggctttgcc aaggacggct acgtcgccgt cgcgccggcg
      781 atgttcgacc gcacccagcg gggctacgac accgggtact cgcagcccga gatccaggcc
      841 ggagtcgcga tcatgcagaa gctcgactgg aagcagaccc tgctcgacgt ggatgccgcc
      901 gtggccgagg cgaagaaagc cggcaaggtc ggcatcgtcg gctactgctg gggcggaacg
      961 gttgcctggc tcgcggctgc caagaccgcg ggcctggcgt gcgccgtgcc ctactacggc
     1021 ggcggcatgc cgaatttcat caacgagaaa ccgaaaatcc cggtgctgtg ccattttggc
     1081 gagcaggacc agtccccgac gctcgagcag gccaaggcca tcgccaaggc gcacccgggg
     1141 atcaccgcgc acttctatcc gggcgccggg cacggcttca actgcgatca acgcccctcg
     1201 ttcaatgccg cggcctccaa gcaggcgcgc gagcgcacgc tcgagttctt ccgcaagcac
     1261 gtcggctgat tagactagat cggccttcgg gccgagcagc gcgcgccgga agcgcgcctt
     1321 gagcagtgcg ccttcgcgcg gcggcagcac gagcgggatc ttctccgcca cgaccttgat
     1381 cgcggcgaag tgcggaccgg gcttgccgtg gatgagctgc ctcagtccgg cgagcgcggc
     1441 tttctcggtg accaggctgc aggaagcgaa ccggcaggcc cgggcgacgg cagcgaggtc
     1501 gacgccgctc gcggtatggg tcgcctgcat gccggtctcg ccgtagcggc cattgtcgat
     1561 cacgacgatg ctcaggttct tcggctgctg cacgccgatg gtagcgaggg agcccaggcc
     1621 catcagcatc tcgccgtcgc cggtgatgac gaggacgcgg cgcttcggct gcgcgagcgc
     1681 gaggcccagc cccatcatcg cggcctgtcc catggcgccc catagcggaa agttgagctc
     1741 atggtcgccc gccgccgtga tgtcccacgc ggtcgagccc aatccggcca ccaccaacag
     1801 attctttctt cctgaaagaa gagacttcac cacatcgcgg cggtgcaggg cgggcttcat
     1861 cggctggcct gctcctggaa gctcttgatg ccgatcacgc gctggggaat cagcaccgcc
     1921 acggacgcgt agctgttgta ggcgaggcgc agcgccgcgt tcaccgtcgg gccgatgtcc
     1981 tgcgcgccgg ccgggtagtg cacgatcgtg cccatggctt cgagcacctg gcgcgtcgcc
     2041 tgccccatcg gcacctgcca gggattgaac tcgccctgct cgccgcgcat cgtcacgagg
     2101 ctcaccagcg gaaagcggca ttccttcacc gtcccgagga cgttgatgac gttgcccacg
     2161 ccgctcgact gcatgagaag cacgcttttc tcgccgccga gccacgcgcc gatggcgagg
     2221 ccgatgcc
//
LOCUS       DATDMP010000456         2778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_184725, whole genome shotgun sequence.
ACCESSION   DATDMP010000456 DATDMP010000000
VERSION     DATDMP010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184725"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2305
                     /locus_tag="VI195_07985"
     CDS             <1..2305
                     /locus_tag="VI195_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6824366.1"
                     /translation="VGGRKGELEFSAPREAFRTTGDALIPWLALAAGLAITVLLAGLA
                     GSLSTSSERARRIAREITQDLRRSQAELEEAQRKTEQLIETLPNPVFFKDRNGTYLGV
                     NKAWEQFFMRPRASIIGLTVHDLYPHAPELAGRMDAADQRLWAEPGTQVYEATLSLPD
                     GTKRDTLYYKATFTGPEGRVAGLIGTIIDITERKQAEKRRGMEHAITRFLASADSPAH
                     AMPQVLDNLCEAMGWTGATLWRREGRELTLLAGAGKGSRAMDGAALLEPQWHAEAGGT
                     LSVPLLLGAEALGVMAFSGVTHAPDPELAAMLQSIGSQIAQYLQRTQAEEALRFVATH
                     DSLTGLPNRVMFSQRVEHAIHQAKRYGRRLAVLFIDLDRFKVINDTLGHEVGDTLLRD
                     VSQRLLQNLRASDTVARLGGDECVVLLEEVNAPVFIAGVARKLIDALTQGFALAGREY
                     HVSASIGVSTYPDDARSAAELLKNADIAMYRAKEQGRNTFEFHAAQHNVHSVERLSLE
                     SGLRRALERGELLLHYQPQVELASGRIVGTEALVRWEHPKHGLLTPASFIDIAEETGL
                     IVPIGQWVLHAACSTQRAWREAGLGRLRMSINLSPRQFMHEGLMSDIGAIVRLSGGDP
                     ALIELEITEGMVMQDPERAVTLLRDMRQIGVHVAIDDFGTGHSSLAYLKRFPVDNLKI
                     DRSFIADIPADRGDAAITQAIIAMAHSLGLKVIAEGVETQEQFDFLAAQGCDEYQGYF
                     FARPMSEEQARAMLEAGAATPRAATAR"
     gene            complement(2292..>2778)
                     /locus_tag="VI195_07990"
     CDS             complement(2292..>2778)
                     /locus_tag="VI195_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6824367.1"
                     /translation="NHLAAELFGLATGTRFNHIPYKGSAPALADLLGGQLTFMFDTVI
                     TSVPQIRSGKLRAFAVSSAKRASSLPEVPTMQEAGFPGFDMSQWQGVLAPAGTPRAVV
                     QRLNAEIVKAMHAPDVHERIAVQGGNEIVTGTPEEFGALIRAELERYAKLIREAKISA
                     Q"
BASE COUNT          449 a          972 c          941 g          416 t
ORIGIN      
        1 ggtcggcggc cgcaaggggg agctcgagtt cagcgcgccg cgcgaggcgt tccgcaccac
       61 cggcgatgcg ctcatcccct ggctcgcgct cgcggccggg ctcgccatca ccgtgctgct
      121 cgcgggtctc gccggctctc tttccacctc gagcgagcgc gcgcggcgca tcgcgcgcga
      181 gatcacgcag gacctgcgcc gcagccaggc ggagctcgag gaagcgcagc gcaagaccga
      241 gcagctgatc gagacgctgc ccaacccggt gttcttcaag gatcgcaatg gcacttacct
      301 cggcgtgaac aaggcgtggg agcagttctt catgcgcccg cgcgcctcga tcatcggcct
      361 cacggtccac gacctctacc cgcatgcgcc ggagctcgcc ggccgcatgg acgccgccga
      421 ccagcggctg tgggccgagc ccggcacgca ggtttacgaa gcgacgctct cgctccccga
      481 cggcacgaag cgcgacacgc tttactacaa ggcgaccttc accggtccgg aaggccgcgt
      541 cgccggactg atcggcacga tcatcgacat caccgagcgc aagcaggcgg aaaagcgccg
      601 cggcatggag catgcgatca cgcgctttct cgcgagcgcc gacagcccgg cgcatgccat
      661 gccgcaagtg ctcgacaatc tgtgcgaggc gatgggctgg accggcgcga cgctgtggcg
      721 gcgcgagggc cgggagctca cgctgctcgc cggcgcgggc aaaggctcgc gcgccatgga
      781 cggcgcagcg ctgctcgagc cgcaatggca cgccgaggcg ggcggcacgc tctccgtgcc
      841 gctgctcctt ggcgcggaag cgctgggcgt catggccttc agcggggtga cgcatgcgcc
      901 cgatccggag ctcgcggcga tgctccagtc gatcggcagc cagatcgcgc agtacctgca
      961 gcgcacgcag gccgaagagg ccctgcgctt cgtcgccacc catgattcgc tgaccgggct
     1021 gccgaaccgc gtcatgttca gccagcgcgt cgagcacgcc atccaccagg ctaagcgcta
     1081 cggccgccgt ctcgcggtgc tgttcatcga tctcgaccgc ttcaaggtga tcaacgacac
     1141 gctcggccac gaggtcggcg atacgctgct gcgcgacgtc tcgcagcgcc tgctgcagaa
     1201 cctgcgggcg agcgacacgg tggcgcgcct gggcggcgac gaatgcgtgg tgctgctcga
     1261 ggaggtgaac gcgccggtat tcatcgccgg cgtggcgcgc aagctgatcg atgcgctcac
     1321 gcagggcttc gcgctcgccg gccgcgagta tcacgtctct gcgagcatcg gcgtcagcac
     1381 ctatccggac gacgcgcgca gcgccgcgga gctgctgaag aacgccgaca tcgccatgta
     1441 tcgcgccaag gagcaggggc gcaacacctt cgagttccac gccgcccagc acaacgtgca
     1501 ctcggtggag cggctgagcc tggagtcagg gctgcggcgg gcgctggagc gaggcgagct
     1561 gctgctgcac taccagccgc aggtggaact tgctagcggg cgcatcgtcg gcacggaagc
     1621 gctggtgcgc tgggaacatc cgaagcatgg actgctgacg cccgcttctt tcatcgacat
     1681 cgccgaagag accggcctca tcgtgccgat cggccagtgg gtgctgcacg ccgcctgctc
     1741 cacgcagcgc gcttggcgcg aagccgggct aggtcggctc cgcatgtcca tcaacctgtc
     1801 gccgcggcag ttcatgcacg agggcctgat gagcgacatc ggcgcgatcg tgcgcctgag
     1861 cggcggcgac ccggcgctca tcgagctcga gatcaccgag ggcatggtga tgcaggaccc
     1921 cgagcgcgcc gtgacgctcc tgcgcgacat gcgccagatc ggcgtgcacg tcgcgatcga
     1981 cgacttcggc accggccact cgtcgctcgc gtatctcaag cgcttccccg tggacaacct
     2041 gaagatcgat cgctcgttca tcgccgacat cccggccgac cgcggcgatg ccgcgatcac
     2101 gcaggcgatc atcgccatgg cgcacagcct cggcctgaag gtgatcgccg aaggcgtcga
     2161 gacgcaggag cagttcgact tcctcgcggc gcagggctgc gacgagtacc agggctattt
     2221 cttcgcgcgg ccgatgtccg aagagcaggc gcgcgccatg ctcgaggctg gcgccgccac
     2281 gccgcgcgcc gctactgcgc gctgatcttc gcctcccgga tcagcttggc gtagcgctcg
     2341 agctcggcgc ggatgagcgc gccaaactcc tcgggcgtgc cggttacgat ctcgttgccg
     2401 ccctgcacgg caatgcgctc gtgaacgtca ggcgcgtgca tcgccttcac gatctcggca
     2461 ttcagccgct gcaccaccgc gcgcggtgtg cccgccggtg ccagcacacc ctgccactgc
     2521 gacatatcga agcccggaaa cccggcttcc tgcatggtgg gcacctccgg cagcgagctc
     2581 gcgcgctttg cgctcgatac ggcaaaggcg cgcagcttgc cggaccgtat ctgcggcacc
     2641 gaggtgatca cggtgtcgaa catgaaggtg agctggccgc ccagcagatc tgccagcgcc
     2701 ggcgcgctgc ctttgtatgg aatgtgattg aagcgggtgc cggtcgcgag cccaaaaagc
     2761 tccgccgcga ggtggttg
//
LOCUS       DATDMP010000457         3074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1407482, whole genome shotgun sequence.
ACCESSION   DATDMP010000457 DATDMP010000000
VERSION     DATDMP010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407482"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VI195_07995"
     CDS             <1..505
                     /locus_tag="VI195_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY6824368.1"
                     /translation="YMDMAKACLRDGTPFGVCLIVQGQEVGEPAIPAPVGTLARIVQW
                     DMPQLGMLHVVAEGTQRFRIQSRRVQPNGLALATVQVLAEEQDAPIAREGERCVRLLE
                     RLIGEQPELFPAPHRLDSSAWVSARLLELLPVPLAAKQELLELDEAAARLARISRLLR
                     ANAQSDS"
     gene            complement(465..1442)
                     /gene="mutY"
                     /locus_tag="VI195_08000"
     CDS             complement(465..1442)
                     /gene="mutY"
                     /locus_tag="VI195_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY6824369.1"
                     /translation="MSLRECYPMDFAQSVVAWQRAHGRHALPWQGSRDPYRVWLSEVM
                     LQQTQVATVIPYYQRFLARFPDVQALARASQDEVLRLWSGLGYYARGRNLHQAAQRIA
                     AQGFPSTARDIEALPGVGRSTAAAIAVFAFGERAAILDGNVRRVLARCFAHSGDLWPL
                     AERLLPSRGIEAYTQGLMDLGATVCSRTRPACERCPVAKLCEANRRGWQERLPAKQAR
                     TALPRKGVTWIVLRHRNRVLVERRPASGIWGALWCFPQGERRAGDRPLQPIEHGFTHF
                     RLRIRPLLREAPANRRQQGRRWMTLGELERAAVPTPVRNLIARLRAAGG"
     gene            1441..2412
                     /locus_tag="VI195_08005"
     CDS             1441..2412
                     /locus_tag="VI195_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824370.1"
                     /translation="MAAFVLRRLAQAVLVMASVAFIAFSLFRFVGDPVLFMLGQDATP
                     EDRVRMSHQLGLDRPFYVQYAAFIERAVHGDFGLSLRQVQPVSTLLKDRLPATLELSL
                     VAALFALAAGIPMGVITALRPRSLLAQALLAVSLIGVSLPTFLIGILLIVLFSVQLGW
                     LPSFGRGDTVQLGWWSTGFLTVSGLKALVLPSITLGLFQMTLIQRLVRSEMLEVMRTD
                     YIRFARARGLTDRAVHFRHALKNTLVPVITITGLQLGSIIAFAIITETVFQWPGMGLL
                     FIQAVTFADVPVMAAYLCLIGLFFVVINLAVDLLYYAVDPRLRVQTG"
     gene            2421..>3074
                     /locus_tag="VI195_08010"
     CDS             2421..>3074
                     /locus_tag="VI195_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY6824371.1"
                     /translation="MTTPVERIRSYHAELKALRRDLHAHPELAFEENRTSALVAERLA
                     SWGIEVHRGLAKTGVVGVVKGRKSNSEKAVGLRADMDCLPVQEANGFAHKSQHAGRMH
                     ACGHDGHTTMLLGAARYLAETRSFDGTAYLIFQPAEEGGGGGHEMVKEGLMERFAISR
                     VFGMHNMPGLPEGHFATTPGAMLASSDNVKITVTGKGGHAGAGPHKAVDSVLIGSQIV
                     "
BASE COUNT          451 a         1096 c         1024 g          503 t
ORIGIN      
        1 ctacatggac atggcgaagg cctgcctgcg cgacggcacg ccgttcggcg tctgcctcat
       61 cgtgcagggt caggaagtcg gtgagccggc gattcccgcg ccggtcggca cgctcgcgcg
      121 gatcgtgcaa tgggacatgc cgcagctcgg gatgctgcac gtcgtggccg aaggcacgca
      181 gcgcttccgc atccagagcc gccgggtgca acccaatggc ctcgcgctcg cgacggtgca
      241 ggtcctggcc gaagagcagg atgctccgat cgcgcgcgag ggcgagcgct gcgttcgcct
      301 gctcgagcgg ctgatcggcg agcagcccga gctcttcccg gcgccgcatc ggctcgactc
      361 gagcgcatgg gtgagcgccc gtctgctcga gctgctgccc gtgccgctcg ccgcgaagca
      421 ggagctgctc gagctggacg aagcggcggc gcgcctcgcg cggatcagcc gcctgctgcg
      481 cgcaaacgcg caatcagatt cctgaccggc gtcggcaccg ccgcgcgctc gagctcgccg
      541 agcgtcatcc agcgccggcc ttgctgccgc ctgttggccg gcgcttcgcg cagcagcggg
      601 cgaatgcgca gccggaaatg cgtgaagccg tgctcgatcg gctgaagtgg ccggtcgccc
      661 gccctcctct cgccttgcgg gaagcaccac agcgcgcccc agataccgct cgccggccgg
      721 cgctcgacga gcacccggtt gcgatggcgc agcacgatcc aggtcacgcc tttccgcggc
      781 agcgcggtac gcgcctgctt cgccggcagt cgctcctgcc agccgcggcg gttcgcctcg
      841 caaagcttcg cgaccggaca gcgctcgcac gcgggccttg tgcgcgagca gacggtcgcg
      901 ccgagatcca tgagcccttg cgtgtacgct tcgatgccgc gcgagggcag cagccgctcg
      961 gcgagcggcc acagatcgcc ggaatgcgcg aagcagcgcg cgagaactcg ccgcacgttg
     1021 ccgtccagga tggcggcccg ctcgccgaaa gcgaacaccg cgatcgcagc cgcggtcgag
     1081 cgcccgacgc cgggcagcgc ctcgatgtcc cgggcggtgc tcgggaaccc ttgcgcggcg
     1141 attcgctgcg cggcctggtg aaggttgcgg ccgcgcgcgt aatagccgag cccgctccag
     1201 aggcgcagca cctcgtcctg cgatgcgcgt gcgagcgcct gcacatcggg aaagcgcgcg
     1261 aggaagcgct gatagtaagg aatgaccgtc gcgacctgcg tctgctgcag catgacctcg
     1321 gatagccaga cgcgataggg gtcgcggctg ccctgccacg gcagggcgtg gcgaccgtgc
     1381 gcccgctgcc acgcgaccac gctttgcgcg aaatccatcg ggtaacattc tcgcagactc
     1441 atggctgcct tcgtcctgcg gcgtctcgca caggccgtgc tggtcatggc ctcggtcgcc
     1501 ttcatcgcct tctcgctgtt ccgcttcgtc ggcgatccgg tgctgttcat gctcggccag
     1561 gacgcgaccc cggaggaccg ggtgcgcatg tcgcaccagc tcggcctcga ccggcccttc
     1621 tacgtgcagt acgccgcctt catcgagcgc gccgtgcacg gcgacttcgg cctgagcctg
     1681 cgccaggtgc agccggtttc gacgctgctc aaggatcgcc tgccggcgac gctcgaactg
     1741 tcgctggtcg ccgcgctgtt cgcgctcgca gccgggatcc cgatgggcgt gatcaccgcc
     1801 ttgcgcccca gatcgctgct tgcccaggcg ctgctcgccg tatcgctgat cggcgtgtcg
     1861 ctgccgacct ttctcatcgg catcctgctc atcgtgctct tctcggtgca gctcggctgg
     1921 ctgccctcct tcgggcgcgg cgacaccgtg cagctcggct ggtggagcac cgggtttctc
     1981 accgtctccg gccttaaggc gctggtgctg ccctcgatca ccctcggtct cttccagatg
     2041 acgctcatcc agcgcctggt tcgctcggag atgctggaag tgatgcgcac cgactacatc
     2101 cgcttcgcgc gcgcccgcgg gctcaccgac cgcgcggtgc atttccgcca cgcgctgaag
     2161 aacacgctgg tgccggtcat caccatcacc gggctgcagc tcggctcgat catcgccttc
     2221 gcgatcatca ccgagaccgt gttccagtgg ccgggcatgg gcctcttgtt catccaggcg
     2281 gtcaccttcg ccgacgtgcc ggtcatggcc gcgtacctct gcctgatcgg ccttttcttc
     2341 gtcgtcatca acctcgcggt cgatctgctc tactatgcgg tggacccgcg ccttcgtgtg
     2401 caaacgggtt agacgaggat atgactactc ccgtagaacg cattcgcagt tatcacgccg
     2461 agctgaaggc gctgcggcgt gacctgcacg cccatcccga gctcgccttc gaggagaacc
     2521 gcaccagcgc gctggtcgcc gagaggctgg cgagctgggg catcgaggtg caccgcggcc
     2581 tggccaagac cggcgtagtc ggtgttgtga aaggacggaa aagcaacagc gaaaaagcgg
     2641 tgggcctgcg cgccgacatg gactgcctgc cggtccagga agcgaacggg ttcgcccaca
     2701 agtcgcagca cgccgggcgc atgcacgcct gcgggcacga cggccacacg acgatgctgc
     2761 tcggcgccgc ccgctatctc gccgagacgc gcagcttcga cggcacggcc tatctcatct
     2821 tccagccggc cgaggaaggc ggcggcggcg gccacgagat ggtcaaggaa ggcctgatgg
     2881 agcgcttcgc catttcccgc gtgttcggca tgcacaacat gccgggcctg cccgaggggc
     2941 atttcgccac cacgccgggc gcgatgctcg cctcctccga caacgtgaag atcaccgtca
     3001 ccggcaaggg cggccatgcc ggcgccggtc cgcacaaggc ggtcgacagc gtgctgatcg
     3061 gctcgcagat cgtg
//
LOCUS       DATDMP010000458         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1296364, whole genome shotgun sequence.
ACCESSION   DATDMP010000458 DATDMP010000000
VERSION     DATDMP010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296364"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..942
                     /locus_tag="VI195_08015"
     CDS             448..942
                     /locus_tag="VI195_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY6824372.1"
                     /translation="MHVTLRVALRVWNLRSRRCFRVIEMSLEEARGRFGLRIIEFSVL
                     GNHLHLVVEADNNDALSRGMQGLNIRIARALNGVMRTNGSVFADQYHPRLLRTPTELV
                     NAIAYVLGNAEHHYGSRGPDRYSSTACNRRRLLSHPRTWLLRMGWRRARRVPQWLTSI
                     GRPD"
     gene            complement(1505..2000)
                     /locus_tag="VI195_08020"
                     /pseudo
     CDS             complement(1505..2000)
                     /locus_tag="VI195_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
BASE COUNT          319 a          723 c          637 g          321 t
ORIGIN      
        1 gtcgtcgacc cgaagccgct gcaactgcgc atcgcgcgcc tggcccggca gatggccagg
       61 gtgcacggcg ggcaggcggg cctgaagccg cgcccatcac cgcggcacgg cggatgaagt
      121 cgctgcgccc cgaatcccgg attcggaggc tcccatgccc gatgcagcat cgaccgccag
      181 tacgccgaga ccgcgctgga ccaggaattc gagaactctc tccgaccagc gcgtcggcgg
      241 cgggtttacg gtgccatcgg tcgattgacc accgatgccg cagtatcgct cgctgcgata
      301 tatccccaat caatgagccg aacgacacag cttgccttgc cgctgccgtc acgcggcggt
      361 cggcggcgcg gggctggacg gaaacccaag ggcgagaggc cgctcgtgtc gcacaaagcc
      421 aggccgcggt tcgaaacggc tgctgccgtg cacgtcacgc ttcgcgtcgc gctgcgcgtc
      481 tggaatctcc gcagccgccg ttgcttccgg gtcatcgaga tgtcgctcga agaggcacgc
      541 ggccggttcg ggctccgcat catcgagttc tccgtgctcg gcaaccacct gcacctggtc
      601 gtcgaggccg acaacaacga tgcgctctcg cgcggcatgc aggggctcaa catccgcatc
      661 gcgagggcgc tgaacggcgt gatgcgcacc aacgggagcg tcttcgcgga ccagtaccat
      721 ccgcgcctgc ttcgcactcc cacggaactg gtcaatgcca tcgcctacgt gctgggaaat
      781 gccgagcacc actatggctc acgggggccg gaccgatatt cgtccacggc gtgcaatcgc
      841 agaaggctcc tttcccatcc gcgcacctgg ctcctgcgta tgggctggcg ccgcgcccga
      901 agagtgccgc agtggctcac gtcgatcggc cggcctgatt gacactctga tgcgtggcct
      961 ttgcagcacg atggaggatc caaccaagcg cttgtgcggg acgtcaggac acgttcgcga
     1021 cggaggcaaa ggcttgcagg gcctttgcgg agagtgcaag agagcggccc gcagcagctc
     1081 caccaaggct gctgcaatct tctggtccga gcggcttcct gcgattcggg ctcgagcccg
     1141 gctgcacgga tttcttcgag caggcgaagg gctccctgat attcgcggtc ggtgctgctt
     1201 gcccaagcca gctgtatcca gccggactcg gttgagcttg gacgccatgc cgcaaggaat
     1261 tcgccgatgc gacccaatag gtacgcaatc tcgtcgactg cttcaacgga aggcgcgcct
     1321 gccagcaact cgagagcgcg gtccgccgcc cctgatcggc gcgaggtact ggggttccgc
     1381 cgcaactatc gcggcgcgtt gactccgtcc gcgcgcgatc gcaaggaacg gctccgaact
     1441 gaccgccgtt ccttgcgtca cactcgtctt acgccgcgac ccgcagctgc gccgccggcc
     1501 gggcgagcgc ggcgtggagc acttcgtcca tccgcttgac caggacgatc tccaactcgt
     1561 cgcggagctg ctgcgggatc tccttgagat cccccgcgca cgcctccggc aggatcacgc
     1621 gcttcagccc cagccggtgc gccgccagca ccttctcctt gatgccgccg acgggcagca
     1681 cgcgcccgcg cagcgtggcc tctccggtca tcgccgtatc gccgcgcacc ggcactccgg
     1741 tcaggagcga gaccagcgcc gtgaacagcg tcacgccggc gctcggcccg tccttgggcg
     1801 tggcccccgc gggcacgtgg acgtgcacgt cccagccctc catcggccgc tccggaaccc
     1861 ccagctccgc cgcgttggcc tgcacgtagc tgagcgccgc ccgcgccgac tccttcatca
     1921 cctcgccgag ctggccgctg aggatgagct gccccttgcc gggcatgcgc agcgcctcga
     1981 cgtagagcac ttcgccgccc
//
LOCUS       DATDMP010000459         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1517367, whole genome shotgun sequence.
ACCESSION   DATDMP010000459 DATDMP010000000
VERSION     DATDMP010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517367"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VI195_08025"
     CDS             <1..974
                     /locus_tag="VI195_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824373.1"
                     /translation="YFYLPKLESHLEARLWNEVFVAAQDALGMARGTIKATVLIETIL
                     AAFEMDEILYELREHCAGLNCGRWDYIFSFIKKFRNRTDFLLPDRAAVTMERHFLKSY
                     VDLLIRTCHRRGAHAMGGMAAQIPIRDDAQANALAMARVRADKLREARAGHDGTWIAH
                     PGLAAIAREAFDEVMSGPNQLAVRREDVRVSARDLLTVPEGAITEEGVRGCIRVGVQY
                     LESWLRGNGCVPLYHLMEDAATAEICRAQLWQWLHHGARTSDGEPVSVARFDRLLTEE
                     LSRIHEEVGAERLTGGVFPTAARLFEQMTKSESFDEFLTLPAYELLS"
     gene            1016..>2291
                     /gene="aceA"
                     /locus_tag="VI195_08030"
     CDS             1016..>2291
                     /gene="aceA"
                     /locus_tag="VI195_08030"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY6824374.1"
                     /translation="MNPNKLLPAADELARNWRDSPRWRGISRVYQPGEVARLRGTIPV
                     EHSIARLTAEKLWRYLNDMPFVNALGALTGNQAMQQVKAGLDAIYLSGWQVAADANLA
                     GEMYPDQSLYPADSVPAVVRRINNALRRADEICHAEGDDSVDWLKPIVADAEAGFGGV
                     LNAFELMKQLIDAGAAGVHFEDQLSSAKKCGHMGGKVLVPTQEAIAKLTAARLAADVC
                     NVPTVLIARTDAEGATLLTSDIDERDRPFIDNSRGRTSEGFFTVKAGMAQAIARARAY
                     APYADLLWCETAKPDLEEAREFAEGVHAQYPGKLLAYNCSPSFNWKRKLDDATIAKFQ
                     RELAAMGYRFQFITLAGFHALNYSMFELARGYKSQQMSAYVKLQQAEFAAEPLGYSAT
                     KHQREVGAGYFDAVTQAVSGGSSSITALAGSTE"
BASE COUNT          405 a          749 c          775 g          362 t
ORIGIN      
        1 cgtacttcta cctgccgaag ctcgagagtc atctcgaggc acggctgtgg aacgaggtgt
       61 tcgtcgcggc gcaggatgcg ctcggcatgg cacgcggcac catcaaggcg accgtgctca
      121 ttgaaaccat actcgcggca ttcgagatgg acgagattct ctacgagttg cgcgagcact
      181 gcgccggact caactgcgga cgctgggatt acatattcag tttcataaag aagttccgca
      241 accgcacgga cttcctgctg cccgaccgcg ccgccgtcac catggagcgg catttcctca
      301 agtcgtacgt ggacctgctc atccgtacct gccaccgccg tggcgcgcac gccatgggcg
      361 gcatggccgc gcagatcccg atccgcgatg acgcgcaggc gaacgcgctg gcgatggcgc
      421 gggtacgcgc cgacaagctg cgcgaggcgc gcgccgggca cgatggcacc tggatcgcgc
      481 atccggggct cgccgccatc gcgcgcgagg ctttcgatga ggtcatgagc gggccgaacc
      541 agctggcggt gcggcgcgag gacgtgagag tgagcgcgcg cgatctgctc acggtgccgg
      601 aaggggcgat caccgaggag ggcgtgcgcg gttgcattcg cgtcggagtg cagtacctcg
      661 aatcctggct gcgcggcaac ggctgcgtgc cgctctatca cctgatggag gatgcggcca
      721 ccgcggagat ctgccgcgcg caactgtggc agtggctgca ccatggcgca cgcacgagcg
      781 atggcgagcc ggtgagcgtg gcacgcttcg atcgcctcct caccgaggag ctcagccgca
      841 ttcacgagga ggtcggcgcc gagcgcctca ccgggggagt gtttccgacc gccgcgcgac
      901 tcttcgagca gatgaccaag agcgagagct tcgatgagtt cctgacactc cccgcttacg
      961 agctgctcag ctgagcgcaa tcaatcgcca atctttccca cggtgcagag gatccatgaa
     1021 cccgaacaag ctacttcccg cggcggatga gctggcgcgc aactggcgcg atagcccgcg
     1081 ctggcgcggc atctcgcgcg tttaccagcc cggcgaggtc gcacggctgc gcggcacgat
     1141 tccggtcgag cactcgattg cgcgcctgac cgcggagaag ttgtggcgtt acctgaatga
     1201 catgccgttc gtgaacgccc tcggcgccct cacgggcaac caggcgatgc agcaggtgaa
     1261 agccgggctc gatgcgatct acctctcggg ctggcaggtg gccgccgatg ccaacctggc
     1321 cggcgagatg tacccggacc agtcgctgta cccggccgac tcggtgcccg cggtggtgcg
     1381 gcggatcaac aacgcgctgc gccgcgcgga cgagatctgc catgccgagg gcgatgacag
     1441 cgtcgactgg ctcaagccca tcgtggccga tgcggaagcc ggcttcggcg gcgtattgaa
     1501 tgccttcgag ctgatgaagc agctgatcga tgcgggtgcg gcgggagtgc atttcgagga
     1561 ccagctctcc tcggccaaga agtgcggcca catgggcggc aaggtgctgg tgcccactca
     1621 ggaggcgatc gcgaagctca ccgcggcgcg gctcgccgcg gacgtctgca acgtgccgac
     1681 ggtgctgatc gcacgcaccg atgcggaggg ggcgacgctg ctcacttcgg acatcgatga
     1741 gcgcgaccga cccttcatcg acaactcgcg cgggcgcacc agcgagggtt tcttcaccgt
     1801 gaaggccggc atggcgcagg cgatcgcgcg cgcccgcgcc tatgcgccgt atgcggacct
     1861 gctgtggtgc gagaccgcca agcccgatct cgaggaagcc cgcgagttcg ccgaaggcgt
     1921 tcacgcgcag tacccgggga agctgctggc ctacaactgc tcaccgtcct tcaactggaa
     1981 gagaaagctc gacgatgcga ccatcgcgaa gttccagcgc gagctggcgg cgatgggtta
     2041 ccgcttccag ttcatcacgc tcgccggctt ccacgcgctc aactactcga tgttcgagct
     2101 cgcgcgcggc tacaagtcgc agcagatgag cgcgtacgtc aagctgcagc aggcggagtt
     2161 cgccgccgag cctctcggct acagcgccac gaagcatcag cgcgaagtcg gcgccggtta
     2221 cttcgatgcc gtcacccagg cggtgagcgg cggcagctcc tcgatcaccg cgctcgcagg
     2281 cagcacggag g
//
LOCUS       DATDMP010000460         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1962743, whole genome shotgun sequence.
ACCESSION   DATDMP010000460 DATDMP010000000
VERSION     DATDMP010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962743"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /gene="ptsP"
                     /locus_tag="VI195_08035"
     CDS             <1..1933
                     /gene="ptsP"
                     /locus_tag="VI195_08035"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY6824375.1"
                     /translation="GLGVRHGEEIEIRASGPDAHNALNALSGVFNAHASPSVPARPLP
                     ASSDVAPAGHATGAAARADAPAAQRARVAGVLRGVAASPGLAVGPALQLHRPEIRVRE
                     AGAGVAAEHAALDGARAEVRARLERAALAAPAGGAAYGTAREIAAAHLELLDDPELIA
                     AARAAIDAGRSAAFAWRAAVRDSADTLRALADTRLRERVDDLLDLETQVLLALEGEAV
                     EPAAALPEQAIVIARELLPSQLIALDGAGLAGIAMAAGGATSHAAILAAAMGIPALVA
                     LGAEALAVPDGTELILDAAGGVLEVAPAAPRVLAARARLAANHAQRAALAAAAQRECR
                     TADGVRIEVLANIGSAAEAQAAVRNGAEGCGLLRTEFLFLDRATAPSETEQTAEYQKI
                     AELLGERPLTVRTLDAGGDKPIAYLPMPPEENPALGLRGIRTSLAWPQLLRTQLRAVL
                     AVHGPARCRVLLPMISDLEELEAVRALLEELRAELGRSEPMPLGVMIETPASALLAER
                     IAAVADFLSIGTNDLTQYTLAMDRGNPQLAVRLDALHPAVLRLIARTAEAARANGRHA
                     AVCGGLASEPLAAPLLVGLGVNELSAVPSVIPQLKARIGSVSLSDCRALAAEALKQPS
                     AAAVRALAAALPTAAEAQA"
     gene            1930..>3017
                     /locus_tag="VI195_08040"
     CDS             1930..>3017
                     /locus_tag="VI195_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEY6824376.1"
                     /translation="MKGALAGLQKVGGALMLPIAVLPIAGLLLRLGQPDLFDSASMTA
                     AGQAIFGNLGLLFAIGVAVGLARENHGAAGLASVVAYLVATRGAEVLISVPPEAVADL
                     SGRARELASAAYKAHELARLTVPVGILSGLMAGALYNRFYNIALPSYLSFFGGRRFVP
                     ILSGLGGLGLAVAFGTQWQHLEQGMDALSRSVLHAGAFGLFAYGVLNRLLIITGLHHI
                     INNIAWYLLGEYHGVTGDQTRFFAGDPSAGIFMSGFFPVMMFGLPAACLAMYHCALPE
                     RRRAVGGLLGSIAFTSILTGVTEPIEFTFIFLAPALYAVHALLTGVAFIVTNALHVRL
                     GFGFSAGLIDYVLNFRLATRPLWLLPLGA"
BASE COUNT          386 a         1089 c         1056 g          486 t
ORIGIN      
        1 gggtcttggc gtgcgccatg gcgaggagat cgagatccgc gccagcgggc cggacgcaca
       61 caacgctctc aacgcactca gcggagtttt caacgcgcac gcgagtccat cggtgcccgc
      121 ccggccattg cccgcatcga gcgatgttgc gcctgccggg cacgccaccg gagccgccgc
      181 gcgcgccgac gcgcccgctg cgcagcgggc gcgcgtggcg ggagtgctgc ggggcgtcgc
      241 ggcgagtccg ggccttgcgg ttggacctgc gctgcagctg caccgacccg agatccgggt
      301 gcgcgaggcc ggagccgggg ttgcggcaga gcacgccgcg ctcgatggcg cacgcgccga
      361 ggttcgcgcc cggctcgagc gtgcggcact cgcggcgcct gcgggcggcg cggcgtacgg
      421 cacggcgcgg gagatcgcgg cggcgcacct cgagctgctc gatgatcccg agctgatcgc
      481 tgccgcccgc gcggcgatcg atgcgggacg cagtgccgcc ttcgcctggc gcgccgcagt
      541 gcgggacagc gcggacaccc tgcgggcgct cgccgacacg cgtctgcgcg agcgagtcga
      601 tgaccttctc gatctcgaga cgcaggtgct gctcgcactc gaaggcgagg cggttgaacc
      661 tgccgcggca cttcccgagc aggcgatcgt catcgcacgc gagctgctgc cctcgcagct
      721 catcgcgctc gatggcgcag gcctcgccgg catcgccatg gcagcgggag gcgcgacctc
      781 gcacgctgcc atcctggctg ccgccatggg catcccggcc ctcgtcgcac tcggtgcgga
      841 ggcgctcgcg gtgcccgatg gcaccgagct gattctcgat gccgccggcg gtgtgctcga
      901 ggtcgcgccc gccgcgcccc gggtgctggc cgcacgtgcg cggctcgccg ccaatcacgc
      961 gcagcgtgcc gcgctcgcag ccgcggcaca gcgcgagtgt cgcactgccg acggggtgcg
     1021 catcgaggtg ctcgccaaca tcggctccgc tgccgaggcg caggccgccg tacgtaacgg
     1081 cgccgagggc tgcggactgc tgcgcaccga gtttctgttc ctcgaccgtg cgacggcccc
     1141 gagcgagacc gagcagaccg cggagtacca gaaaatcgcc gagctgctcg gcgaacgtcc
     1201 gctcaccgta cgcacactcg atgccggcgg cgacaaaccg atcgcttacc tgcccatgcc
     1261 gcccgaagag aatccggcgc tcggcttgcg cggtattcgc acgagtcttg cgtggccgca
     1321 gttgctgcgc acgcagctgc gcgcggtgct cgccgtgcac ggcccggcgc gctgccgcgt
     1381 gctcctgccg atgatcagcg acctggagga gctcgaggcg gtgcgggcgt tgctcgagga
     1441 gttacgggcc gagctcggac gaagcgagcc gatgccgctc ggtgtgatga tcgagacgcc
     1501 agcctcggcg ctgctcgccg agcgcatcgc ggcggtggcg gactttctct cgatcggcac
     1561 caacgacctc acgcagtaca cactcgccat ggaccgcggt aatccgcagc ttgcggtgcg
     1621 gctcgatgcg ctgcaccccg ccgtgctgcg cctcatcgcg cgcaccgccg aggcagcgcg
     1681 cgccaatggc cgccatgccg cggtgtgtgg tggccttgcc tccgagccgc tcgcggcgcc
     1741 gctactcgtg gggctcggag tgaatgagct ctcggcggtg ccttcggtca tcccgcagct
     1801 caaggcgcgt atcggcagtg tgtcgctgag tgactgccgc gccctcgccg cggaggcgct
     1861 caagcaaccg agcgccgccg cggtgcgtgc actcgccgca gcgctgccga ccgccgccga
     1921 ggcccaggca tgaaaggggc gctcgctggt ctgcagaagg tcggcggcgc gctcatgctg
     1981 ccgatcgcgg tgctgcccat cgcggggctg cttctgcggc tcgggcagcc ggacctgttc
     2041 gattccgcct cgatgaccgc cgccggccag gcgatcttcg gcaaccttgg cctgctgttc
     2101 gcgatcggcg tcgccgttgg gcttgcgcgc gagaaccacg gcgccgcggg actcgcgagc
     2161 gtggtggcct acctcgtggc cacgcgcgga gcggaagtgc tgatcagcgt gccgcccgag
     2221 gcggtcgccg acctgagcgg tcgggcgcgt gagctggcga gcgcggccta caaggcgcac
     2281 gagctcgcgc ggctcaccgt gccggtcggc atcctctccg ggctgatggc cggggcgctt
     2341 tacaaccgtt tttataacat cgcgctgccg agttacctga gtttcttcgg cggccggcgc
     2401 ttcgtgccga tcctgagcgg tctgggtggc ctcgggctcg cagtcgcctt cggcacccag
     2461 tggcaacacc tcgagcaggg catggacgcg ctcagccgct cggtgcttca cgcgggggcg
     2521 ttcgggctgt tcgcctatgg tgtgctcaac cgcctgctga tcatcaccgg gctgcaccac
     2581 atcatcaaca acatcgcctg gtacctgctc ggcgagtatc acggtgtcac cggtgaccag
     2641 acacgcttct tcgccggcga cccgagcgcc gggatcttca tgtcgggatt cttcccagtc
     2701 atgatgttcg ggctgccggc cgcgtgtctg gcgatgtacc actgcgcgtt gcccgagcgg
     2761 cgccgcgcgg tcggcggcct gctcggctcg atcgcattca cctccatcct caccggcgtc
     2821 accgagccaa tcgaattcac cttcatcttt ctcgccccgg cgctctatgc cgtgcacgcg
     2881 ctgctcaccg gtgtcgcctt catcgtcacg aatgcgctgc acgtgcggct cggcttcggc
     2941 ttctccgccg ggctcatcga ctacgtgttg aatttccgcc tggccacgcg cccgctgtgg
     3001 ctgctgccgc tcggagc
//
LOCUS       DATDMP010000461         3545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2185783, whole genome shotgun sequence.
ACCESSION   DATDMP010000461 DATDMP010000000
VERSION     DATDMP010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185783"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VI195_08045"
     CDS             <1..253
                     /locus_tag="VI195_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824377.1"
                     /translation="DPGRRLKLIYLPNPTLPPADTVLTDDLILDAVRGGTIVRLLGAG
                     VPAGSEWDTQYWRLRTSWLQALNRLKILAEKRPAPGTGS"
     gene            250..621
                     /locus_tag="VI195_08050"
     CDS             250..621
                     /locus_tag="VI195_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6824378.1"
                     /translation="MTGFVLRAVISAIGLWLATRWVRGVYIDSATTLLLAGLLLGVVN
                     AVVRPVAFVLTLPLTIVTLGLFLLVLNAAMVALVAWILPGFHLGGFRAALLTWLIVWI
                     TGWVGSALIGQRGYERYERRS"
     gene            complement(629..2605)
                     /locus_tag="VI195_08055"
     CDS             complement(629..2605)
                     /locus_tag="VI195_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY6824379.1"
                     /translation="MNTESRAELTLRGLILGVLLTVVFTAANVYFGLKAGLTFSTSIP
                     AVVLSMALLRWLGNTTAQENNIVQTVASTAGTLSSIIFVLPGFVMIGWWTGFPYWQSV
                     AICATGGILGVMYSIPLRRALVTDSDLPYPEGTACAEVIKVGSGDVTDKDSVESGGKG
                     LKAVLVGSLVSALFLVMVQTRLFLGDVAHSFRLGSDPRAPLTGFDFQLSFALFGIGHL
                     VGISCGIAMFVGALIGWGWGVPHFSALHAAAGDAGDLAGATWSHYVRYVGAGAIAVSA
                     IWTLMKLVKPVIGGLKSAMASSRARKAGKGDTLARTEHDIPVDQVGLVSLLCILPICW
                     LLWNFASGAGLGEHALLLTIGGIVIVVVLGFLVSTVCGYMAGLIGASNSPLSGIGILV
                     VATTALFLVVTVKSSLPPQMGKALVAYALFVTAVVFNVAAIANNNLQDLKTGQLVEST
                     PSKQQWALVIGVIASALVIPPVLDLVNKAYGFAGAPGASAHALPAPQAGLISALGQAV
                     IQSNPEKWHLMGLGSLVGAGVIALDWLLSKTSRSMRLPPLAVGLGIYLPTASTLMVTV
                     GALVGWWFDRGADRTPRPDATKQLGVLLASGLIVGESVLAVIFTALVALTGNPFPIGV
                     VGDSFATPSEWLGGIAFVAVIYALYRWAGRLLPG"
     gene            2766..3119
                     /locus_tag="VI195_08060"
     CDS             2766..3119
                     /locus_tag="VI195_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824380.1"
                     /translation="MAKVLIGIVVLALLALAGFTWATLNWSYSDGERAGYVQKFSRRG
                     WLCKTWEGEMAMVTMPGTVAEKFYFTVPSDAIAAQLNANIGRRMTLHYEQHKWVPSSC
                     FGDTEYFVTRVRVTE"
     gene            complement(3125..>3545)
                     /locus_tag="VI195_08065"
     CDS             complement(3125..>3545)
                     /locus_tag="VI195_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824381.1"
                     /translation="GRDPPAVAKFGVALLLLGLGFLVMYVAAQRVLAGRLVLPAWLIV
                     TYFLHVCGEMCLSPVGLSNMSKLAPPRFVGQAMGMWFLSLALGGNLAGQLTGEYDASH
                     LESLPALFLKIFWYGVIGGAVMLALARPLRRLIEGGR"
BASE COUNT          609 a         1179 c         1250 g          507 t
ORIGIN      
        1 cgatcccggt cggcgtctca agctcatcta cctgccgaac ccgacactgc cgcccgccga
       61 caccgtgctc actgacgacc tgattctcga tgcggtgcgc ggcgggacca tcgtgcgcct
      121 gctcggcgcg ggggtgccgg cgggcagcga gtgggacacc cagtactggc ggctgcggac
      181 cagctggctg caggccctca accggctgaa gatcctcgcg gagaagcggc ccgcgccggg
      241 gacagggtca tgaccggctt cgtgctgcgc gcggtgatca gcgcgattgg gctgtggctc
      301 gcgacccgct gggtgcgcgg cgtgtacatc gacagcgcga cgacgctgct gctcgcgggg
      361 ctgctgctcg gggtggtgaa tgccgtggtg cggccggtgg ccttcgtgct caccctgccg
      421 ctcacgatcg tgacgctcgg cctgttcctg ctggtgctga atgccgccat ggtggcgctg
      481 gtcgcctgga tcctgccggg ctttcacctc ggcggcttcc gggccgccct cctcacctgg
      541 ctgatcgtgt ggatcaccgg ctgggtgggc tcggcgctga tcgggcagcg cggctacgag
      601 cgctacgagc gccgctcgtg aggcgcgctc agccgggcag cagccggccc gcccagcggt
      661 agagcgcgta gatcaccgca acgaaggcga tgccgccgag ccactccgag ggcgttgcaa
      721 acgaatctcc caccacgccg atgggaaacg gattaccggt gagggcgacg agcgccgtga
      781 agatcaccgc cagcacgctc tcgccgacga tgagccccga agcgagcagc accccgagtt
      841 gcttcgtggc gtcgggtctg ggggtccggt cggcgccgcg gtcgaaccac cagccgacca
      901 gcgcgcccac ggtgaccatg agcgtcgatg ccgtgggcag gtagatcccg aggccgacgg
      961 cgagtggcgg cagacgcatg gagcggctgg tcttcgagag cagccagtcg agcgcgatca
     1021 cgccagcccc tacgaggcta cccaagccca tcaagtgcca tttttccggg ttgctctgga
     1081 tgaccgcctg gccgagggcg gagatgaggc ccgcctgggg tgcgggcagg gcgtgagccg
     1141 aggcgccggg ggcgcctgca aagccgtacg ccttgttcac caggtcgagc accggcggga
     1201 tcaccagcgc gctcgcgatc acgccgatca ccagcgccca ctgctgcttg gagggcgtgg
     1261 attccaccag ctggccggtc ttcagatcct gcaggttgtt gttggcaatg gcggccacgt
     1321 tgaatacgac cgccgtgacg aacagcgcgt acgccacgag cgccttgccc atctgcgggg
     1381 ggagcgacga cttgacggtc accaccagga acagcgccgt ggtggccacc accaggatgc
     1441 cgatccccga gagcgggctg ttggaggcgc cgatcagccc cgccatgtag ccgcacacgg
     1501 tcgaaacgag gaagccgagg accaccacga tgacgatgcc gccgatggtg aggagcagcg
     1561 catgctcgcc gaggcccgca ccgctcgcga agttccacaa cagccagcag atcggcagga
     1621 tgcacagcag cgacacgagt cccacctgat ccaccgggat gtcgtgctcg gtgcgcgcca
     1681 gggtgtcccc cttgccggcc ttgcgtgccc gggaggaagc catggcgctc ttgaggccgc
     1741 cgatcaccgg tttcacgagc ttcatgagcg tccagatggc cgacacggcg atggcgccgg
     1801 cgcccacgta acgcacatag tggctccagg tggcccccgc caggtctccc gcatcgcccg
     1861 cggcggcgtg cagtgcgctg aagtgcggca ctccccagcc ccagccgatg agggcaccga
     1921 cgaacatggc gatgccgcag ctgatgccta ccaggtggcc aatcccgaac agcgcgaagg
     1981 agagctggaa atcgaaaccc gtgagcggcg cccgcgggtc agaacccagg cggaagctgt
     2041 gagcgacgtc cccgaggaag agccgcgtct gcaccatcac caggaacagg gcggagacga
     2101 gcgaaccgac cagcacggct ttgaggccct tgccgccgga ttccaccgag tccttgtccg
     2161 tcacgtcgcc ggaaccgacc ttgatcacct cggcgcaggc ggtgccctcg ggatagggca
     2221 ggtccgagtc ggtcaccagt gcgcggcgca gggggatgga atacatgact ccgaggatgc
     2281 cgccggtggc gcagatggcc accgactgcc agtagggaaa gccggtccac cagccgatca
     2341 tgacgaagcc gggcagcacg aagatgatgg aggagagagt gccggcggtg gaagccaccg
     2401 tctgcacgat gttgttctcc tgggcggtgg tgttgccgag ccagcgcaac agcgccatgg
     2461 agagcacgac cgccgggatg gaggtcgaga aagtgagccc cgccttcagc ccgaagtaga
     2521 cgttggcggc ggtaaacacc acggtgagca gcacgccgag gatcagcccc cgcagcgtga
     2581 gctcggcccg cgattccgtg ttcatggttc gacccccggg tgttggtgtt ataggcggcg
     2641 cttcgggcgg gcgcggccgg cgcgcggcag tggctccatg gcagctcatc ttagcctcgc
     2701 ttcagctgcg gcgcgcggta tccttggctg caggcgcgga cgcggtccgt ggcggcaggc
     2761 ggtccatggc gaaggtactg atcggcatcg tggtgttggc gctgctcgcg ctggcgggct
     2821 tcacctgggc gacgctcaac tggtcgtact ccgacggcga gcgcgccggc tacgtgcaga
     2881 agttctcgcg ccgcggctgg ctgtgcaaga cctgggaggg cgagatggcc atggtgacca
     2941 tgccgggcac ggtggcggag aagttctact tcacggtgcc gagcgacgcg atcgcggcgc
     3001 agctcaacgc caacatcggc aggcgcatga cgctgcacta cgagcagcac aagtgggtgc
     3061 cgagctcctg cttcggcgac accgagtact tcgtgacccg cgtgcgggtc accgagtaga
     3121 gcgcttatct cccgccctct atcagccgcc tgagcggccg cgcgagcgcc agcatcacag
     3181 ccccgccgat caccccgtac cagaagatct tcaggaacag cgccgggagc gactcgaggt
     3241 ggctcgcgtc gtactccccg gtcagctggc cggcgaggtt gccgccgaga gcgagcgaca
     3301 ggaaccacat gcccatcgcc tggccgacga aacgcggcgg agcgagcttg ctcatgttgg
     3361 acagacccac cggggacagg cacatctccc cgcagacgtg gaggaagtag gtgacgatca
     3421 gccaggccgg cagcaccagg cggccagcga gcacgcgctg cgcggcgacg tacatgacga
     3481 ggaacccgag cccgaggagc agcagcgcga cgccgaattt ggcgaccgcc ggcggatcgc
     3541 gcccg
//
LOCUS       DATDMP010000462         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_962311, whole genome shotgun sequence.
ACCESSION   DATDMP010000462 DATDMP010000000
VERSION     DATDMP010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962311"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="VI195_08070"
     CDS             complement(<1..1985)
                     /locus_tag="VI195_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY6824382.1"
                     /translation="MIAKRSSRALAVLLALPSAAFALGLGDIRLLSALNAPLDAEIDL
                     VDVTPEEMSTVQAQLASRDTFTRYGLDWPPYLSGVQVRTVRMSDGREVIKLKSNDPIS
                     DPFVTLLVEVNWARGKLVREYTMLLDPPVFTPGQPQVASAPVTAPSAGAGPREGSIAR
                     ATEPAAPAPPPVPAAAAAPVLPTPVAAPAPAAEAAPAAETAASRSEAGSARAEAAGPA
                     AEAAPSPADSGASANASPPPEPAASSSSAVGAAAPPPPSASSAESEGSHTVRHGETLS
                     QIASGVAGEKVSSAHTRSWMLAIYQANPRAFQGNMNVLHSGAVLRIPDAETAAAVSPA
                     EASSEIRRQYAAWRGTSAASSESAAATQPGRLKLVPPSESASAGSAPGASAGESGALQ
                     GRVRELEGQLAESKRLLEMKEAELARMQAQLAAKQQPAAPPPAAAQPPAPSAPAQQPP
                     AAQVAPPPAPPPPPAPEEKPPEAIAPPPPPVVARAPAPTPKPAAPPSEPSGSFLGTLV
                     GYWWVLLIAAVAAGAVAALRILRSRRASQFDASLGRLAVAGADAELDLGFQEHATPSP
                     AASAPAAGGAARNFAASDTAVRTKPSQAREPAFVVEETGTHERPRLTPSGAPAAPTAR
                     HVATDETISSETAINLDQGDPLAEADFHMAYGLYDQAA"
BASE COUNT          327 a          683 c          780 g          306 t
ORIGIN      
        1 gcggcctggt cgtagaggcc gtaggccatg tggaaatcgg cttcggccag cggatcgccc
       61 tggtcgagat tgatggcagt ctcgctgctg atcgtctcat ccgtcgcgac atgccgtgcg
      121 gtcggtgccg ccggcgcgcc gctcggagtc agccgtggac gctcgtgcgt gccggtctcc
      181 tcgacgacaa acgcgggctc acgagcctgc gacggcttcg tgcggacagc agtgtctgac
      241 gctgcgaagt tgcgggcagc gccgcccgct gccggagccg aagcggcagg gctcggagtg
      301 gcgtgctctt ggaatccgag atcgagctcg gcatcggcgc ccgcaaccgc gagccggccg
      361 agggaggcat cgaattgcga cgcacgccgc gaccgcagga tgcgcaacgc agcgacggcg
      421 cccgccgcca ccgcggcaat cagcagcacc caccagtagc cgaccagcgt tccaaggaac
      481 gatccgctcg gctcggaagg cggtgccgca ggctttggtg tcggtgcggg tgcgcgtgcc
      541 acgacgggcg gcggcggcgg agcgatcgct tcgggtggct tctcttcggg cgccggtggg
      601 ggcggcggtg cgggcggtgg cgccacctga gctgccggcg gctgctgggc gggcgccgaa
      661 ggcgcggggg gttgggcagc ggccggaggc ggcgccgcgg gctgttgctt ggcggcaagc
      721 tgcgcctgca tgcgcgcgag ctccgcttcc ttcatttcca gcagccgctt cgactcggcg
      781 agctggcctt cgagctcacg cacccggccc tgcaatgcgc cggactcgcc ggccgatgcg
      841 ccaggggctg aaccggcaga ggcgctttcg gagggcggca cgagtttcag acgacccggc
      901 tgcgtcgcag cggcggattc ggaggaagcc gcggaggtac cgcgccacgc cgcatattga
      961 cggcggatct cgctcgaggc ttccgccggc gacacggcgg ccgcggtctc agcatccggg
     1021 atgcggagca ccgcgcccga gtgcaggaca ttcatgttgc cctgaaaggc gcgtgggttc
     1081 gcctgataga tggcgagcat ccagctgcgc gtatgcgcgg aactgacctt ctcgccggcg
     1141 acgcccgagg cgatctgcga aagcgtctcg ccatggcgga ccgtgtgcga gccctcgctc
     1201 tccgccgaac tcgcggacgg tgggggcgga gctgccgcgc cgacagcgct cgatgagctg
     1261 gcagcaggct ctggcggagg cgatgcgtta gcggaagcgc cagagtcggc aggtgatggt
     1321 gcggcttcgg cggccggccc ggccgcctcc gcgcgcgccg atcccgcctc agagcgtgat
     1381 gcggcggtct cggccgcggg cgcagcttcg gctgcgggag caggcgccgc aacaggcgtc
     1441 ggaagtaccg gtgcggcggc cgccgcgggg actggaggcg gtgccggcgc agcgggctcg
     1501 gtcgcgcgcg cgatggaacc ttcgcgtgga cccgcacccg ccgagggagc cgtgaccgga
     1561 gcgctcgcga cctgcggctg gccgggagtg aataccggcg gatcgagcag catggtgtat
     1621 tcgcgcacca gcttgccgcg cgcccagttc acctcgacga gcaaagtaac gaagggatcg
     1681 gagatcgggt cgttcgactt cagcttgatg acttcgcgcc catcggacat gcgcacggtg
     1741 cgcacctgaa cgcccgagag atagggcggc caatcgaggc catagcgggt aaaggtgtcc
     1801 cgcgaggcga gttgcgcctg cacggtactc atttcttccg gcgtcacatc gaccaggtcg
     1861 atctccgcgt cgagtggcgc attgagcgcc gacagcagtc gaatgtcccc aagacccagg
     1921 gcaaacgcgg cggacggcag ggccagcagt actgccagcg cccggctgga gcgtttggcg
     1981 atcatcaggt caccccggca ggcattaatt gggagaaatg tctagtaaaa acaacaaggt
     2041 gggtcataca ctgcgacccc cctggcaccc cggcggcaat ataacttaaa tagccc
//
LOCUS       DATDMP010000463         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_630159, whole genome shotgun sequence.
ACCESSION   DATDMP010000463 DATDMP010000000
VERSION     DATDMP010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630159"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VI195_08075"
     CDS             complement(<1..329)
                     /locus_tag="VI195_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824383.1"
                     /translation="MSRAGVAGTIWALCATVAATIVVHARYSTDLSAFLPRRATATQR
                     LLVEQLNEGPAAHLIIAAISGGDAGTRAAVSRYMTERLRANASFLAIGNGDETQLQRD
                     REFLFEHR"
     gene            complement(326..931)
                     /locus_tag="VI195_08080"
     CDS             complement(326..931)
                     /locus_tag="VI195_08080"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEY6824384.1"
                     /translation="MRWLTLPCAVLGCTLALGDCPQPCAAAEPPTDPAAFEQLLQRLS
                     ARRHGHVAFTEVQQLSILDRPLHSSGELLYDAPGRLEKRTLEPRPEDLLLEDGTVTVE
                     RGHRRRIVALRDLKQAAPLIESLRATLAGDRAALERYFTVHFSGNLDRWSLELTPADP
                     LLRRSVRRIHIGGEQDRVRTVEIYQSDGDSSTLTIGADLAP"
     gene            complement(928..1938)
                     /locus_tag="VI195_08085"
     CDS             complement(928..1938)
                     /locus_tag="VI195_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824385.1"
                     /translation="MTRTPLEARCAPRGSSRQPGELKQVTDGARRADDLAQRSGAAWT
                     TEPERGSAVLLEIMMFVALRLGRPFARALLYFIAAYFFLSGPRARRHGRQYLRRALGR
                     EPTLLERYRQMFAFAATILDRLYLTRGRYELLDISIEGEELMHAALARGRGAFLLGAH
                     LGSFEMMSAVGRRQPGLRVALAMYTGYLSKVALRFGFGPPGSGTEVIPLGQLDSMLRI
                     RDSLEEGKFVGMLADRSFGDTPGQRVPFLGEPAVFPTGPMRAAAALRRPVIFMAGLYR
                     GGNRYRVVFRPLADFSGASPLTRERAVHAGIERYVALLEECCRSDPYNWFNFYDFWGV
                     DA"
BASE COUNT          354 a          677 c          707 g          291 t
ORIGIN      
        1 cgatgctcga acaggaattc gcggtcgcgc tgcagctgcg tctcatcacc gttgccgatt
       61 gcgaggaagg aagcattcgc gcgcagccgc tccgtcatgt accgcgacac tgcggcgcgc
      121 gtgccggcat cgcccccgga gatcgcggcg atgatgaggt gtgcggcggg gccttcattc
      181 agctgctcga cgagcaggcg ctgcgtcgcc gtcgcccgcc gcggcaggaa cgccgagagg
      241 tcggtgctgt agcgggcgtg tacgacgatg gtggcggcaa cggttgcaca gagtgcccag
      301 atcgtgcccg caacgccggc acgactcatg gggcgagatc agccccgatg gtgagcgtgg
      361 agctgtcgcc atcgctctgg tagatctcga ccgtgcggac gcggtcctgc tcgccgccga
      421 tgtgaatgcg tcgcacgctg cgccggagca acggatcggc gggtgtcaac tcgagactcc
      481 accgatcgag atttccgctg aagtgcaccg tgaaatagcg ctcgagggcg gcgcggtcgc
      541 ccgcgagcgt ggcgcgcagg ctctcgatga gcggcgcggc ctgcttgaga tcccgcagcg
      601 ctacgatgcg ccgccgatgg ccgcgctcca ccgtgaccgt cccgtcctcg agcagcaggt
      661 cctccggccg tggctcgagg gtgcgtttct cgagccggcc cggggcgtcg tacagcagct
      721 cgccggagga gtgcaggggc cgatcgagta tcgagagctg ttgtacctcg gtgaaggcca
      781 cgtgcccgtg gcgccgcgcg gagagacgct gcagcagctg ctcgaaggcc gccggatccg
      841 tggggggttc agccgccgca caaggctgcg ggcagtcgcc caaagccagt gtgcagccaa
      901 gcaccgcgca aggcagggtc agccacctca cgcgtccact ccccagaagt catagaagtt
      961 gaaccagttg tagggatcgc tccggcagca ctcctcgagc agcgccacgt aacgctcgat
     1021 gcccgcgtgt accgcgcgct cacgagtgag cgggctcgct cccgagaagt cggcgagcgg
     1081 ccggaacacc acccggtagc gattgccgcc gcggtaaagg cccgccatga agatcaccgg
     1141 acgacgcagt gccgccgcgg cccgcatcgg gccagtcggg aagactgccg gctcaccgag
     1201 gaaaggcacc cgttggccgg gcgtgtcgcc gaagctgcgg tcggcgagca tcccgacaaa
     1261 cttaccttcc tccagagaat cgcggatgcg cagcatgcta tcaagctggc cgagtggaat
     1321 gacttccgtg ccacttcctg gcgggccgaa gccgaagcgg agcgcaactt tgctcaggta
     1381 accggtgtac atcgcgagcg ccacgcgcag tccgggttga cgccgcccaa cggcgctcat
     1441 catctcgaag ctgccgagat gcgcgccgag cagaaacgct ccgcggccgc gcgcgagcgc
     1501 cgcgtgcatg agctcctcgc cctcgatcga gatatcgagc agctcgtagc gcccgcgtgt
     1561 cagatacagc cgatcgagaa tggtggccgc gaaggcaaac atctgccggt atcgctcgag
     1621 gagagtcggc tcacggccga gtgcgcgacg caggtattgc cgcccgtggc ggcgagcgcg
     1681 cggcccggag aggaagaagt aagcggcaat gaagtagagc aatgcccggg cgaacggccg
     1741 gccgagacgc agcgcgacga acatcattat ctcgagcaac actgcactgc cgcgttcggg
     1801 ctctgtcgtc cacgcggcgc ccgagcgctg cgcgagatca tccgcacgcc gcgcgccgtc
     1861 ggttacctgc ttcagctcgc ccggttgccg ggagctgccg cgcggcgcgc accgggcctc
     1921 aaggggcgtg cgggtcatcg ggaatcaaca ccgcgtgagc gaccggctcg ccggcccgca
     1981 gaacgctgaa gcggatcgag ccgttaggca gccgctcgtg cccaagcgt
//
LOCUS       DATDMP010000464         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1297331, whole genome shotgun sequence.
ACCESSION   DATDMP010000464 DATDMP010000000
VERSION     DATDMP010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297331"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..266)
                     /gene="rpmE"
                     /locus_tag="VI195_08090"
     CDS             complement(51..266)
                     /gene="rpmE"
                     /locus_tag="VI195_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY6824386.1"
                     /translation="MKASIHPEYKEITVSCSCGNSFKTSSTLGHDLQIEVCSNCHPFY
                     TGKQKIVDTAGRVDKFRRKYASAAGAK"
     gene            complement(624..1835)
                     /gene="hemW"
                     /locus_tag="VI195_08095"
     CDS             complement(624..1835)
                     /gene="hemW"
                     /locus_tag="VI195_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY6824387.1"
                     /translation="MASLRAPPLALYVHFPWCVRKCPYCDFNSYTLHGELPQSPYVAR
                     LARDIEAQAALVEGREVGSVFFGGGTPSLFAPEAIGRVLESARRHLTVTADAEVTLEA
                     NPGAIERGAFREYRAAGVTRVSLGAQSFEARALHALGRIHSPADTRRAAAELHAAGID
                     NFNLDLMYGLPGQEPAAAVRDIEEALALSPAHLSHYQLTLEVGTVFAARPPPLPDEDA
                     CAAMLEECRARLGVRGFAQYEVSAYARARAQCRHNLNYWTFGDYLGVGAGAHGKLSFA
                     AAGRIVRTTQLREPRRYLSAPDTELVQRTIAQRELPFEFMLNALRLNTGFEMTTFTAR
                     TGLEAEAIAGPLRAAVDAQLLLTTAAGYRPSARGLRFLNDLLLYFLPETPEKTGPSAL
                     SMATPEVAARA"
     gene            complement(1925..>2178)
                     /locus_tag="VI195_08100"
     CDS             complement(1925..>2178)
                     /locus_tag="VI195_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824388.1"
                     /translation="ASGSKTITEVVTVTTAWTFTLDCTQGTQHAHSEVTVDILTPSTG
                     KGGGGGGALDAATLLSLLSSLVIGSVFVRNPRVHSAAWS"
BASE COUNT          389 a          684 c          719 g          386 t
ORIGIN      
        1 cttctcggtc tttatcttcg cggtggcggc gcgcccgcgg agcgggcggc ttacttcgcg
       61 ccggcggcgc tcgcgtactt gcggcggaac ttgtcgacgc gaccggccgt gtcgacgatc
      121 ttctgcttgc cggtatagaa aggatggcag ttcgagcaca cctcgatctg caggtcgtgc
      181 ccaagagtcg agctcgtctt gaaggagttg ccgcagctgc agctgaccgt gatctctttg
      241 tactcggggt gaatcgacgc tttcatggca gactcgctgt tcggcgtggc gctggactct
      301 taaatggggc ggcaagcacg acagttaaag ggcgcgggag tttaacggca cccccgcgcg
      361 ggctcaagcg cgcccgccag gcgcctttcg cagcgcagtt ccttatccac agaagctgtg
      421 gacaactctg tggactggcc cttccgtagg cacgcgattt cgcagccaga ttgcataatt
      481 gttacgttga tcaaaaaatg accaggtaat tttcacctgt gacctcaatg agttatgaac
      541 agcacatgcg gcggcttagc cgatatgcca cgtaatttgc tcattctcaa acgtagcctc
      601 ggcggcctgt gaataaaggt atttcaggcg cgggcggcaa cctcgggcgt ggccattgac
      661 aacgcggaag gaccagtctt ttcaggcgtt tccggcaaga aatagagcag caaatcgttg
      721 agaaaacgca gcccgcgggc actcggccga tagcccgcgg cggtggtgag cagtaattgc
      781 gcatcgaccg cggcgcgcag cgggcccgcg atcgcctcgg cctcgagacc ggtccgagcg
      841 gtgaaggtcg tcatctcgaa acccgtgttg aggcgcagcg cattcaacat gaattcgaat
      901 ggcagttcgc gctgcgcgat cgtccgctgc acgagttccg tatcgggcgc cgacagatag
      961 cggcgcggct cgcgcagctg cgtcgtgcgc acgatgcgcc cggccgcagc gaagctcagc
     1021 ttcccgtgcg cgcccgcccc gacccccaga tagtcaccga aggtccagta gttgaggttg
     1081 tgccggcact gtgcgcgcgc acgcgcgtag gcggagacct cgtactgggc aaagccacgc
     1141 acgccgagcc gcgcgcgaca ctcttccagc atggcggcgc acgcatcctc atcaggcagc
     1201 ggcggcgggc gcgccgcgaa aacggtgccg acctcgagcg tgagctggta gtgcgagaga
     1261 tgcgctggag atagggccag cgcctcctcg atgtcccgaa ccgccgcggc gggctcctgg
     1321 ccgggcaaac cgtacatgag atcgagattg aagttatcga tgccggccgc gtgtagctcc
     1381 gcggccgcgc gccgggtgtc cgcgggcgaa tggatgcgcc cgagtgcatg cagggcgcgc
     1441 gcctcgaagc tctgcgcgcc gagtgaaacg cgcgtcacgc ccgcggcccg atattcgcga
     1501 aacgcaccgc gctcgatggc gccgggattc gcttcgagcg tcacctccgc atccgccgtc
     1561 acagtcaggt gccggcgcgc cgattccagc acccgtccga tggcctcggg cgcaaagaga
     1621 ctgggtgtgc cgccgccgaa aaagacgctc cccacctcgc gcccctcgac cagcgccgcc
     1681 tgtgcttcga tgtcccgcgc cagccgcgcg acgtagggtg actgcgggag ctcaccatgc
     1741 agtgtgtagg agttgaagtc gcagtacgga cacttgcgca cgcaccaggg aaagtgcacg
     1801 tacagggcga gcggcggtgc gcgcagggag gccatgtgct cggatccagc ctgaggggtg
     1861 gtgattttag tccgttcggc aggcgcgctc atcgaccgcg atgcgggtgt ggtccgcagg
     1921 aagctcacga ccaggctgct gagtgcactc gtgggttgcg cacgaagaca gaaccgataa
     1981 cgagcgacga cagcagactc agtagtgtcg cagcgtcgag cgctccgccg ccaccgccgc
     2041 ccttcccggt gctgggcgtg aggatgtcaa ccgtgacctc tgagtgggcg tgctgagtgc
     2101 cttgtgtgca gtccagcgta aacgtccacg ctgtggtcac ggtaaccact tcagtgatcg
     2161 ttttgcttcc gctcgccg
//
LOCUS       DATDMP010000465         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_408485, whole genome shotgun sequence.
ACCESSION   DATDMP010000465 DATDMP010000000
VERSION     DATDMP010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408485"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VI195_08105"
     CDS             <1..895
                     /locus_tag="VI195_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824389.1"
                     /translation="IDYAARDRRWTQGNLQHSRVLGFRGLHPLSRVHLLTGIVAYVSS
                     PMWFALLLLSSLLSAIEAAKKPEYFWPGFQSLFPHWPQIRSGEIAVLFGLTLAVLLLP
                     KILGAILAIGDRTVRRQFGGAAALGVGLLVEQLFSMLLAPTMMLFHSTFVAQTLLGRS
                     VSWNAQDRSERGLSFREAFRRQKWHLALGIAWGAAMLYLAPQFFWWLTPVLAGLFAGI
                     PLTMWTSRTSAGLLFRRMGLLLTPEETAPPPELVTLNRAGEDIAGLESATGLVPPPVP
                     PSVPPPVRPRPAYYPPQRKSG"
     gene            963..1532
                     /locus_tag="VI195_08110"
     CDS             963..1532
                     /locus_tag="VI195_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY6824390.1"
                     /translation="MMKRSLSFLVGALLLMGAAMSRADDFTDTIQMFKDAGASKGFFD
                     NSYGYAVFPGIGKGGFVVGGAHGNGRVYRKGAHTGEVSMTQVSVGFQIGGQAFREIIF
                     FEDERAYSEFTKGSFEFAADATAVAITAGAEASAGTTGASAGASGGQRDATTRGKYYK
                     GMAAFVIVKGGAMAGVSVGGQKFSFTPKS"
     gene            1787..1948
                     /locus_tag="VI195_08115"
     CDS             1787..1948
                     /locus_tag="VI195_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEY6824391.1"
                     /translation="MLHYAVVFFVIAIIAAIFGFGGIAAGAAGIAKILFIVFLIGAII
                     TFLVGRSRV"
     gene            complement(1967..2284)
                     /locus_tag="VI195_08120"
     CDS             complement(1967..2284)
                     /locus_tag="VI195_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824392.1"
                     /translation="MAFTPGVAWTPGLRTSGVAVDPAWVHARTATGKNEAEIRARMSI
                     PSACRPGPARLTGRGQFVRRLFLVLRRWFLVRRDIGTRRDAAGYRRTLTVSWASEGPL
                     VVE"
BASE COUNT          379 a          808 c          818 g          434 t
ORIGIN      
        1 catcgactat gcggcgcgtg accggcgctg gacacagggg aacctgcagc actcgcgcgt
       61 gctcgggttc cgcgggctgc acccccttag ccgtgtccac ctgctcaccg gtatcgtcgc
      121 ctatgtgagt tccccgatgt ggttcgcgct gctcttgctg agctcgctgc tctcggccat
      181 cgaggcggcg aagaaaccgg aatacttctg gccgggcttt cagagcctgt tcccccactg
      241 gccgcagatt cgcagcggcg agatcgcggt gcttttcggg ttgacgctcg ccgtactgtt
      301 gctgccgaaa attctcggcg ccattctcgc gatcggtgac cgcaccgtgc gccggcagtt
      361 cggtggcgcg gccgcccttg gcgtggggct gctcgtcgag cagctcttca gcatgctgct
      421 cgccccgacc atgatgctgt tccactcgac tttcgtcgcg cagacgctgc tcggccgctc
      481 ggtgtcgtgg aatgcgcagg accgcagcga gcgcggcctg agctttcgcg aggccttccg
      541 ccgccagaaa tggcacctcg cgctcggtat agcctggggt gcggcgatgc tgtacctcgc
      601 gccgcagttt ttctggtggc tgacgccggt gctggcggga ctgttcgccg gtattccgct
      661 gaccatgtgg acgagccgca cgagtgccgg cctgttgttc cggcgcatgg gtctgctgct
      721 gacgccggag gagaccgcgc cgccgccgga gctcgtgacc ttgaaccgcg ccggcgagga
      781 catcgcaggg ttggagtccg caaccggtct cgtgccacct cccgtgccgc cgtcggtgcc
      841 gccgccggtg cggccgcgcc cggcctatta tcccccgcag cgcaaatcgg gctgatgagc
      901 cgagcgagct gcatgcccgc acgtgcgggc gtcgatgact gcatgagtca accaggagag
      961 cgatgatgaa gcgaagcctt tctttccttg tcggtgccct actgctgatg ggcgcggcga
     1021 tgagccgggc ggacgatttc accgacacca ttcagatgtt caaggacgcc ggagccagca
     1081 agggcttctt cgacaactcc tacggctatg cggtcttccc gggcatcggc aagggcggtt
     1141 tcgtggtcgg cggcgcccac ggtaacggcc gcgtgtaccg caagggggcc cacacgggcg
     1201 aggtgtccat gacgcaggtg agcgtcggct tccagatcgg cggtcaggcg ttccgcgaga
     1261 tcatcttctt cgaggatgag cgcgcctaca gcgaattcac caagggcagt ttcgagttcg
     1321 ccgcggatgc gaccgcggtc gccatcactg cgggggcgga ggcgagcgcc ggcacgacgg
     1381 gtgccagtgc gggcgcgagc ggcgggcagc gggacgcgac cacgcgcggc aagtactaca
     1441 agggtatggc cgcgttcgtc atcgtgaagg gcggtgcaat ggccggggtg tcggtcggcg
     1501 gccagaagtt ctcgtttacg ccgaaatcct gaggatccac gccctcagtc gcgcgccgcg
     1561 cgctcgatga gcggcacgac cgcggaagcg gatgccgcaa tgcaataccc caacgtgaac
     1621 tcggggcctg agtttcgcgc gcatattaac ctgcacctgc gggcgtcagc cgacgcccgt
     1681 agcctttgtc ctacacgcgc ttcagtctcc gccccagtgc caaccgagcc gcgagcagcc
     1741 aaagttcgca tcaacatcaa ctcgcccggc gcaagcggag gaacccatgc tgcactacgc
     1801 cgttgtgttt ttcgtcatcg cgatcatcgc tgctatcttc ggttttggtg gtattgccgc
     1861 cggagccgcc ggcatcgcga agatcctgtt catcgtcttt ctgatcggcg cgatcattac
     1921 cttcctggtt ggccgctcaa gggtgtaggc gcgccccatg ggcacctcat tcaacaacca
     1981 atgggccctc gctcgcccac gacaccgtga gcgtgcggcg ataaccggct gcatcgcgcc
     2041 tggtgccgat gtcgcggcga acgaggaacc agcgtcgcag aacgaggaac aggcgtcgca
     2101 cgaattgccc ccgcccggtg agccgtgcgg gccccgggcg gcaggcggac gggatggaca
     2161 tccgggcacg gatttcggcc tcgtttttgc cggtcgcagt gcgcgcatga acccacgctg
     2221 gatcgaccgc gacacctgag gtgcgcaacc ccggggtcca ggccaccccc ggggtgaagg
     2281 ccaccaggcc gcaccgacat tcaacctgag ccatgcgtgg cggcgccgcc cggtggggcg
     2341 gcaccgcacc gttgcgctcg tgctgcggct caccgcacct tgtaaactcc ccgcgggggt
     2401 cggggaggca cgatgaacga cgaagtggcg acgatcggc
//
LOCUS       DATDMP010000466         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_297930, whole genome shotgun sequence.
ACCESSION   DATDMP010000466 DATDMP010000000
VERSION     DATDMP010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297930"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VI195_08125"
     CDS             complement(<1..692)
                     /locus_tag="VI195_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824393.1"
                     /translation="MPSNDLEVLLRSRVPIVVVESRDEARVLKSLASACTLLSAPPGA
                     PTALPAPRVGAARGALPLFQWTVTDGLKRVDVELGPPLRMLAQPADVLKHIRATTLAG
                     AYALLDFHPYLSDPTIVRLLKDIAQDYERCARTVVLIGYQIEIPHELEHLAARVVLAL
                     PDRNERRQLVEEVARQWMRANPRQAVRIDSRALELLVENMSGLSAADTQRLARKAIYE
                     HGALQLSDVPMV"
     gene            complement(718..1293)
                     /locus_tag="VI195_08130"
     CDS             complement(718..1293)
                     /locus_tag="VI195_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY6824394.1"
                     /translation="MESFLVSLSTVAVAEMGDRTQFLSLMLAAHYRRPWPILAGVSCA
                     TLANHLLAGLIGVRLGQFLTPAMLDAAVGISMLGMALWALRPDARPQDERGRPAGAFV
                     ATLVAFFVAEIGDKTQIATVALAAAYSNLVTVVAGTTAGMLLANVPVVFLGKAFSDRL
                     PLTALHRVASALFLILGALFIYRALGHTLHP"
     gene            complement(1330..>2193)
                     /gene="rfaE2"
                     /locus_tag="VI195_08135"
     CDS             complement(1330..>2193)
                     /gene="rfaE2"
                     /locus_tag="VI195_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY6824395.1"
                     /translation="TLTPNLHELELACGVTLSVEDAAGIATAARGLMGSAGLESMVVT
                     LGSRGMLVVPCAGPEVSVPALRHEVYDVTGAGDTAIAVLTLALATQSPLLEAAQLANA
                     AAGVAVCQVGAVAVSAASIRDALASHPDGKLLSRPELAARAATWRAEGKRIVFSNGCF
                     DLLHAGHLALLSQAAKLGDVLVLAINSDASVRRLKGCERPLVPQAERAALLAALTFVD
                     AVTIFDEDTPLAVLQTVKPHVLVKGGDYQPAEVIGRELVEEAGGRVVIVPLTPEKSTS
                     SLVARIRGARH"
BASE COUNT          391 a          752 c          750 g          300 t
ORIGIN      
        1 atcaccatcg gcacgtccga gagctgcagg gcgccgtgct cgtagatcgc cttgcgggcg
       61 aggcgctggg tatcggcggc ggacaggccg ctcatgttct ccaccagcag ctcgagcgcg
      121 cgtgaatcga tgcgcaccgc ctggcgggga ttggcgcgca tccactgccg cgccacctcc
      181 tcgaccagct ggcgccgctc gttgcggtcg ggcagcgcca gcacgacgcg ggcggcaagg
      241 tgctcgagct cgtgcgggat ctcgatctgg tagccgatca gcaccacggt acgggcgcaa
      301 cgctcgtaat cctgcgcgat atccttcagc aagcgcacga tggtgggatc gctgagatag
      361 ggatggaagt cgagcagcgc gtaggcaccg gcgagtgtgg tcgcgcggat gtgcttgagg
      421 acatcggcgg gctgagccag catgcgcagc ggtggcccga gctccacatc gacacgcttc
      481 aagccgtcgg tcaccgtcca ctggaaaagc ggcaaagcgc cgcgcgctgc gcccacgcgc
      541 ggcgccggca acgccgtggg cgcccccggg ggtgcgctga gcagcgtgca tgcgctcgca
      601 agcgacttca gcacccgtgc ttcgtcacgc gactcgacca caacgatcgg aacgcgcgag
      661 cggagcagaa cctccagatc gttgcttggc atggcggcgc ggagcttagc agactcctca
      721 cgggtgcagc gtgtgaccga gcgcacggta gatgaagagc gcgccgagaa tcagaaacag
      781 tgcactcgcc acgcggtgca gcgcagtcaa cggcaagcgg tcggagaagg ccttgccgag
      841 gaataccacc ggcacattcg cgagcagcat gccggcggtg gtgccagcga ccaccgtgac
      901 caggttggag taggccgcgg cgagcgccac ggtggcgatc tgtgtcttgt caccgatctc
      961 ggcaacgaag aaggccacga gcgtcgcaac gaacgcgccg gccggacgcc cgcgctcgtc
     1021 ctgcggccgc gcatcgggcc tgagtgccca caacgccata ccgagcatgc tgatgcccac
     1081 cgccgcatcc agcattgccg gcgtgaggaa ctggccgagc cgcacgccga tgagaccggc
     1141 gagcaggtgg ttggcgagcg ttgcgcacga cacgccggcg agaatcggcc acggcctgcg
     1201 gtagtgcgcc gccagcatga gcgacaggaa ctgtgtgcgg tcgcccatct ccgcaacggc
     1261 caccgtggag agcgaaacca gaaacgattc catcagcagg cggcaatcgc tcgatgccgg
     1321 cggctgactt cagtggcggg caccgcgaat gcgcgccacc agagagctcg tcgatttctc
     1381 gggcgtcaac ggcacgatca ccacgcgccc accggcttcc tccaccaact cgcggccgat
     1441 gacttccgca ggttggtagt ctccgccctt caccagcacg tgcggtttga cggtctgcaa
     1501 caccgcgagc ggggtgtcct catcgaagat ggtcaccgca tcgacgaagg tgagcgcggc
     1561 gagcagcgcg gcacgctctg cctggggaac gagcggacgc tcgcagccct tgagccggcg
     1621 caccgaggca tcactgttga tcgccagcac gagcacatca ccgagtttcg cggcctggct
     1681 gaggagcgcc agatgaccgg cgtgcagcag atcgaagcag ccgttgctga acacgatgcg
     1741 tttaccctcg gcgcgccagg tggcagcgcg cgcggcaagc tccgggcgcg atagcagctt
     1801 accgtccggg tgcgaagcga gcgcatcgcg gatgctcgcg gccgacaccg ccactgcgcc
     1861 gacctggcac accgccacac cggccgcggc attggcaagc tgcgccgcct cgagcagcgg
     1921 cgactgcgtc gcgagcgcga gggtcagcac cgcgatcgcg gtatcgccgg cgccggtgac
     1981 gtcgtacacc tcatggcgca aggccggcac cgacacctcc gggccagcgc acggcaccac
     2041 gagcatgccg cggctgccga gggtcaccac catggactcg agtcccgcgc tccccatgag
     2101 cccgcgcgcc gctgtggcga tgccggcggc gtcttctacc gacagcgtca ctccgcacgc
     2161 cagctcgagc tcgtgcaggt tgggcgtcag cgt
//
LOCUS       DATDMP010000467         5683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_409177, whole genome shotgun sequence.
ACCESSION   DATDMP010000467 DATDMP010000000
VERSION     DATDMP010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409177"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1369
                     /locus_tag="VI195_08140"
     CDS             173..1369
                     /locus_tag="VI195_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY6824396.1"
                     /translation="MRRIALRLPDDGVWTGGVNYVENVSRALLSHGEFGYEPVVLCSP
                     QADAQLQARFEALLGTRLTRDAQLPRGRRAGLLGAVLLGRNHAMRAVCERSGCDLILE
                     AADFFGWRFPTPCLAWVPDFQDRHLPQLFSHWARYRRSLGLSLQLAAGRTVLLSSEDA
                     RKDCERFYPEARGKTSVARFAVLPALAPGEHDSHLRSRYDLPARFFYLPNQFWAHKNH
                     AGVIEALRLLRTRGGGVVVATSGSPREPRQAGHFERLRARVAATGLREHFRFLGNVPA
                     RDVALLMRASVALLNPSFFEGWSTTVEEGKSLGVRMVLSDLAVHREQIGSGADFFDPH
                     APDAIAACLERVWHDSREPVGLPEQQAAAASARQRVREFAHDFTRACERTLAARSRRS
                     ASPGVL"
     gene            1359..2642
                     /locus_tag="VI195_08145"
     CDS             1359..2642
                     /locus_tag="VI195_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6824397.1"
                     /translation="MFSDDLHGSAPALRGRRRLRLLFVEQFYYPDGWGGAELPLDLTS
                     HLARRGFAVEVICGTDQYAPLEGEPPADPRLLGVRIRRIPRLLPGNVHRGKLLRQLWF
                     YLALAPLVLLRRPPDVFVAQTNPPLGVILVAVAAKLWRRPLIIIAMDIYPEVLIAHGV
                     AGSDAVSTRLLRGAFGWAYRRAQRIVALGPVMRERLEAKGISSRRIVEIPNWATGTQG
                     LITGADNKLRLEWGLADKFVLLYSGNLGLAHEFETFLQGVRQAAKSVPALRLVFIGRG
                     SRLQEVRRRIAELDLEAIVRFNDLLPPARLPESFGLAQLAVVTLQPAFAGLVVPSKLP
                     GYMARGMPVLYVGPDSDIERFVKGSGGGVCQRTGDVQAVRATLIELAHDRERLAALGV
                     RGRRYYEEQFAVQHGLMRYEAVIREVLDGASLPVS"
     gene            2639..3568
                     /locus_tag="VI195_08150"
     CDS             2639..3568
                     /locus_tag="VI195_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6824398.1"
                     /translation="MTVVAVSGAGGFLGRHLSQELLARGHRLIALSRAQLLSPQLSEI
                     LAGVQTVVHLAARAHVLAEASDDPQAEFRTSNVVLTQALALVAQRVGVQRFIFLSSAG
                     VLGATSPPEGVAEHAAPHPHDAYTASKLQAEEWLTSQLDARMGLVILRPPLIYGPGAK
                     GNFMRLLRLAMKGWSLPVGALRAPRSLVAVRNIVDLIGVLVADASPVRTTMLVADRET
                     TSVADLYRIGAREAGHRPWLAPVPPALIRLMLGLSGRSTDVARLTLPFVLRPQLAQAQ
                     FNWVPPYSQQAELRRTARCELDAAARADWQGAK"
     gene            3731..5563
                     /locus_tag="VI195_08155"
     CDS             3731..5563
                     /locus_tag="VI195_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824399.1"
                     /translation="MTCCGSGSGSGNGITNASNASLQETGPADKIVPVPGTATAVVAI
                     FPDGKANYSPDGFNLGGGGATISAYSGQLQVLDVVAVASGVDALFSDGSVFFSPDGKD
                     LGGGGRSVSAYSGGQAITSLVPVGAGVDAIFANGASVYYSPDGLNLGGGGRSILIYSA
                     GHQGVAHIIAVGPGDAVDTLFADGSVFYSPDNRNLADGGATVSATGGSHLTVQHLVQV
                     GGGVLAEFGNGSVYLSPDGQNLAGGGRTIQVLSWNTAIADGPFAARDSAYGAEFLGRL
                     WLSGGYADSTNSNSCFTTCSFFDLWSSTDIQGATWNPTPSFATTTTPDPRDASPVVNG
                     GVQDVAVPTDFYDPYSALVVWNGELTAIGSSVWRSSDGVSWVRNNQPDGSASPGPLSQ
                     RASENSRAAVAAASLFVIQTDTGEVYRSSDAHAAVWADLGAIPGFAPRCGAAVFAMSG
                     KIWIEGGGACDYSQVYHDIWSSSDGTTWMQNGATAAWSARMWPCVASGDGITWLAAGY
                     APTDWNNTGGTVVPRYGANHADVWYTRDGSNWKQFKADVGSSLSDDGGLEPRHAPTCY
                     VAGAAPARNLVIIAGTGGSDPNDASAEVLNSIRAVPLPQAASLP"
BASE COUNT          901 a         1804 c         1940 g         1038 t
ORIGIN      
        1 gcctacaacg gccgaagcgc atcgaatttt ccggagtccg ccgctccggc gatcctgcgg
       61 ccctggtggc tgatacggag cggcttgccg cgctcggtca cgcgtgccgc gtcccgttgc
      121 cggtcggcgt gcagcgctat gccgactggt tcaagcgtgc gcaggaccag tggtgaggcg
      181 gatcgcacta cgcctgccgg acgatggagt ctggacgggc ggtgtcaact atgttgaaaa
      241 cgtcagccgc gcgctcctca gccatggtga attcggttac gagccggtcg tgctctgcag
      301 tccgcaggcc gatgcgcaac ttcaggcacg cttcgaggcc ctgctcggga cgcgcctgac
      361 gcgcgatgcg cagctcccgc gcggccggcg cgccgggctg ctcggcgccg tgctgctcgg
      421 gcgcaatcat gcgatgcgcg ccgtgtgcga gcgcagcggc tgcgacctca tcctggaggc
      481 ggccgatttc ttcggctggc gctttcccac gccctgcctc gcctgggtac cggactttca
      541 ggaccggcat ctgccgcagc tgttctcgca ctgggcgcgt tatcgaaggt cgctgggatt
      601 gagcctgcag ctggccgccg ggcgcaccgt gttgctcagc agcgaggatg cgcgcaagga
      661 ttgcgagcgc ttctacccgg aggcacgggg caagactagc gtggcgcgct ttgccgttct
      721 gcctgcgctt gcacccggcg agcacgactc gcacctgcgc agcagatatg acctgccggc
      781 gcgctttttc tacctgccga atcaattctg ggcgcacaag aaccacgccg gcgtgatcga
      841 agcgctgcgc ttgctgcgca cgcgtggcgg gggtgtcgtc gttgcaacga gcggcagtcc
      901 acgggagcct cggcaagcgg gccacttcga acgcttgcgc gcgcgggtcg cagcgacagg
      961 tctcagggag catttccgct tcctcggcaa tgtgccggcg cgcgatgtgg cgttgctcat
     1021 gcgcgcgagc gtcgcgctgc ttaatccatc cttcttcgaa ggatggagca cgacggtcga
     1081 ggagggcaag tcgctcggcg tgcgcatggt gctctcggat ctcgccgtac atcgcgagca
     1141 gatcggcagc ggcgcggatt tcttcgatcc gcatgcgccg gacgcgatcg ccgcgtgtct
     1201 ggagagagtc tggcacgatt cccgcgagcc ggtggggctg cccgagcagc aggccgccgc
     1261 ggccagcgcc cgacagcgcg tgcgtgagtt tgcacacgac ttcacgcgag cctgcgaacg
     1321 tacgctcgcg gctcgctcac gccgcagtgc gagccccggt gttctctgac gaccttcacg
     1381 ggagcgcgcc ggcactccgc gggcgcaggc gcctgcgcct gctcttcgtc gagcagttct
     1441 attatccgga cggatggggc ggcgcggagc tgccactcga tctcacctcc cacctggcac
     1501 gtcgcggttt cgccgtcgag gtgatctgcg gcaccgatca gtacgcgcct ctcgagggtg
     1561 agccgcccgc cgatccacgc ctgctcgggg tccgtatccg gcgcattccg cggctgctgc
     1621 ccggcaacgt tcatcgcggc aagctgctgc ggcagctgtg gttctacctg gcgctggcac
     1681 cgttggtact cctgcggcgt ccgccagatg tattcgtcgc gcaaacgaat ccaccgctcg
     1741 gggtgatact cgtggccgtg gcagcgaagc tgtggcgcag gccgctgatc atcatcgcga
     1801 tggacattta tccggaggtg ctcatcgcgc acggcgtcgc gggcagcgac gccgtatcca
     1861 cgcgactgtt gcgcggcgct ttcggctggg catatcgacg tgcgcaacgc atcgtcgcgc
     1921 tcgggccggt gatgcgcgag cgcctagagg ccaagggcat cagtagccgg cgcatcgtcg
     1981 agattccaaa ctgggccacg ggaacccagg gactgatcac gggcgccgac aacaagctgc
     2041 gcctggagtg gggactcgct gacaagttcg ttctgctgta ttcgggcaat ctcgggctgg
     2101 cgcacgagtt cgagactttc ctgcagggtg tcaggcaagc cgcgaaatcg gtgccggcac
     2161 tgcgactggt gttcatcggg cgcggcagtc ggctgcagga ggtgcgccgg cgcatcgctg
     2221 agctggatct cgaggcgatc gtgcgcttca acgatctact gccgcccgcg cggctgcccg
     2281 agagcttcgg cctcgcacag ctcgcggtcg tgacgctgca accggcgttt gccggtctgg
     2341 tggttccgag caagctgccg ggttatatgg cgcgaggcat gccggttctt tatgttggac
     2401 ccgacagtga catcgagcgt ttcgtgaagg ggtcgggcgg gggcgtgtgt cagcgtaccg
     2461 gtgacgttca ggcggtccgg gcgacgctca tcgaactcgc ccacgatcgc gaacgcctgg
     2521 cagcactggg agtgaggggg cgccggtact atgaagagca atttgccgtg cagcatggcc
     2581 tgatgcgcta tgaggcggtc atccgcgagg tgctggacgg cgcgagtctg cccgtgtcat
     2641 gaccgtcgtg gcagtttccg gtgccggcgg tttcctcgga cggcacctgt cgcaggagct
     2701 gctcgcgcgc ggtcatcgcc tcatcgcgct ttcccgcgcg caactgctgt caccgcagct
     2761 cagtgagatc ctcgcgggag tacaaacggt agtacacctc gccgcgcgtg cgcacgtgct
     2821 cgcggaagcc agcgacgacc cgcaggcgga gttccgcacg agcaacgtcg tgctcacaca
     2881 ggcgctagcg ctggtggcgc aacgcgtcgg cgtgcagcgc ttcatttttc taagctccgc
     2941 aggggtgctc ggagcaacgt caccgcccga gggagttgca gagcatgcgg cgccgcatcc
     3001 tcacgatgcg tataccgcct cgaagctgca ggctgaagag tggctcacga gtcagctcga
     3061 tgcgcggatg gggctcgtca ttctgcggcc gccactcatc tacgggccag gcgcaaaggg
     3121 caacttcatg cggttgctgc gactggcaat gaaaggttgg tcgctgcccg tgggagcctt
     3181 gcgcgccccg cgctcgctgg tggcggtacg caacatcgtc gatctcatcg gcgtgctggt
     3241 ggccgacgct agtccggtcc ggacgacgat gctggttgcc gaccgcgaga cgacctcggt
     3301 tgccgacttg tatcgaatcg gagcccgtga ggccggtcat cgcccgtggc ttgcgccagt
     3361 gccaccggcg cttatacgat tgatgttggg tttgagcggg cgaagtaccg atgtggcccg
     3421 gctcacgctg ccctttgtgc tgcgcccaca gctcgcgcaa gcgcaattca actgggtccc
     3481 tccctactca cagcaagccg agctacggcg gaccgctcgt tgtgagctgg acgccgcggc
     3541 gcgcgcagat tggcaaggtg caaagtaagc cggagcgaaa cgccgggttt cgccgtgctg
     3601 aagaggacgg gtcgtggttg gagaagcaat gagtcgaagt tgcgcgcacc atcgtcgtcg
     3661 atggttactg ttaaggtcat ctgcggcagc tttgatcagg tcttgtctgc ttggggtcat
     3721 agggcttgga ttgacatgct gcggcagcgg ctcaggctcc ggcaacggca tcaccaatgc
     3781 cagcaacgcc tcgcttcagg aaaccggtcc cgccgacaag atcgtgcccg ttccgggcac
     3841 ggcgacggcc gtggtcgcga tcttccccga tggcaaggcg aactacagtc ccgacggctt
     3901 caatctcggc ggtggcggcg cgaccatttc cgcctacagc gggcagctgc aggtgctcga
     3961 cgtcgtcgcc gtggcgagcg gcgtcgatgc gctgttctcg gacggatcgg tgttcttcag
     4021 ccctgacggg aaggatctcg gcggtggtgg caggagcgtc agcgcctaca gcggagggca
     4081 ggcgattacc agcctggtgc cggtaggcgc tggcgtcgac gcgatcttcg ccaacggcgc
     4141 gagtgtctac tacagtcccg acggactgaa cctcggtggc ggtggccgca gcatcctcat
     4201 ctactccgct gggcaccagg gcgtcgcgca catcatcgca gtgggaccag gcgatgcggt
     4261 ggacaccttg ttcgccgatg gatctgtctt ctatagcccc gacaatcgca acctggccga
     4321 tggcggcgcg acggtctccg cgaccggggg aagtcatctg acggtccagc atctcgtcca
     4381 ggtcggcgga ggtgtgctgg ccgagtttgg caacggctct gtgtatctga gcccggacgg
     4441 acaaaatctc gccggtggcg gccgcacgat tcaggtgctg tcatggaata ctgcaatcgc
     4501 ggacggaccg tttgcggcgc gagacagcgc gtacggggca gagttcctcg gccgcttgtg
     4561 gctcagcggc ggatatgccg actccaccaa cagtaacagc tgtttcacaa cctgcagctt
     4621 cttcgatctg tggtcgtcca cggacataca gggtgcaacc tggaacccaa cgccttcgtt
     4681 tgccaccacc accacacccg atccgcggga tgcctcgccg gtggtgaacg gtggggtaca
     4741 ggacgtggcg gtcccgacgg acttttacga cccgtacagt gcgctggtcg tgtggaacgg
     4801 cgagctcacc gcgatcggct cgagcgtgtg gcgatccagc gacggcgtga gctgggtccg
     4861 taacaatcag ccagacggca gtgcatcgcc agggccgctc tcacagcgcg ccagcgagaa
     4921 ctcacgcgct gcggtagcgg ccgcttctct gttcgtcatc cagacggaca ccggtgaggt
     4981 ctatcgcagc agcgacgcgc acgctgcggt gtgggccgat ctgggtgcca tccctgggtt
     5041 tgcgccccgg tgcggagccg ccgtattcgc catgtccggg aagatatgga tcgagggtgg
     5101 tggcgcctgc gactattcgc aggtgtatca cgacatctgg tcctcatcgg acggcacgac
     5161 atggatgcag aatggcgcca ccgctgcatg gtcggcgcgc atgtggccgt gcgttgcaag
     5221 cggcgacggc attacctggc tcgccgccgg ctacgcacct accgactgga acaacaccgg
     5281 tggcacagtg gtcccgagat acggcgccaa tcatgcggac gtctggtaca cgcgcgacgg
     5341 aagcaactgg aagcagttca aggccgatgt cggctcgagt ctgtccgatg acgggggact
     5401 cgagccccgg cacgcgccca cctgctacgt cgcgggcgcc gcgcccgccc ggaatctcgt
     5461 gatcatcgct ggcaccggag gcagtgaccc aaacgatgca agtgccgagg tgctcaacag
     5521 cattcgtgcc gtaccgttgc cgcaggccgc ctcgctgccg taagtcagtc cgcgcgtgcc
     5581 gggtgctaaa cttgcagcac cgatgagctg aggggtgctg cttgcccaag actgcgctga
     5641 tttgcggggt tactggccag gacggagcct atctcgccga att
//
LOCUS       DATDMP010000468         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_298710, whole genome shotgun sequence.
ACCESSION   DATDMP010000468 DATDMP010000000
VERSION     DATDMP010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298710"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VI195_08160"
     CDS             <1..549
                     /locus_tag="VI195_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY6824400.1"
                     /translation="VTIEYARAGAIRSVQVAVASEEAGGRRVGRIRVLQPAGVSYPPD
                     MLLHTDLAAGAALVRAGDEAWSMTMLQARLFWRMVLGRVSLKNLSGPLSIAEFAGDSA
                     EAGIASFLSFLVLISLSLGFLNLLPIPILDGGQILFQLVEWLKGSPLSERAQALGQQV
                     GIALLIVLMGVALYNDIARQFG"
     gene            747..3368
                     /gene="bamA"
                     /locus_tag="VI195_08165"
     CDS             747..3368
                     /gene="bamA"
                     /locus_tag="VI195_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY6824401.1"
                     /translation="MATPRAGHRSAPLALLAALALAARCALVPAQTPIAPEQSTSAPE
                     PGPPAPTQAPPAPAQTPPVPATTPTAPEQTTPVPEQTPPRPAPAATVPADFTVTDIKV
                     EGLQRVSEGTVYNYLPVNIGDHLTPQRVREAIRALYATGFFRDVQLRRDGSTLIVVVL
                     ERPSIESFEITGNKDIKTEDLQKSLRGVGLATGKTFDRSVLEDVTQYLTDQYFARGKY
                     GVRINSSVEEEAGNRVKIKIDIKEGSRAKIRQINIVGVTKYPQKAILDTLELKTPNWL
                     SWYRQDDRYSRESLQGDLEKIRNFYMDRGYANFNLASTQVAITPEKEDIFITVNIEEG
                     QVYKVAEIKLAGTFVVPKRELEQLLVIHPGDTFNRKRITTSQELMQNRMGRDGYAFAK
                     VEPVPTADNANHTVALTFFVDPGNRVYVRNITFSGANRINDEVLRRELRQLEGGWLSN
                     TSLERSKQRIQRLPYVKTVDFETTPVAGAPDLVDVNYKIEEGPASQLSGGIGWSETYK
                     FMLNASYADADFLGTGQRVAINLNGGAFSKVYSISHTNPYTNIDNLQRTISVTYSDVT
                     QFVSSSSTFSSQTLSVGPTWAYPITEFMYMRMGIAFNSSHLLTNSLSSAEQAQQWVQQ
                     NGHSFSRLAHDDTTNNEYVFYGTDFTTTEAVIGWDWDTRNRTLFADRGMRQSISGAIT
                     FPFSTVKYWIGNYQFVKYVPIAHGFFFSFLEGVDYGKPLSGTTAIPPYRNFFGGGPDN
                     VRGFRESRLGPKDQFGNPYGGNMRITSQNELIFPMPSKYAQTARVSLFFDVGGVFYEG
                     NTIKFYGPDNISPVDYSFHGWPSLRRSVGVAVQWLAPLGLFRFSFGVPLNAQHGNGIQ
                     TWGDEKEGFQFSVGNAF"
     gene            3567..4151
                     /locus_tag="VI195_08170"
     CDS             3567..4151
                     /locus_tag="VI195_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY6824402.1"
                     /translation="MNLAIKSRVGVWMMAASAALAALPAAAAEPKIGVVDFQRLAIES
                     PQGKAVMESMRAEFAPRERTLQAQGQAVKAKEEKLQKDGATMSEEQRARAEKDLRDGA
                     RDFERAKGEFQDDMNARRNEELSRLQRTIVEEVRTYGKAQGFDLILAQEGVIYNASAS
                     DITPAVLTALQARGGSAAAAKPAAPPPPAKPPTQ"
BASE COUNT          766 a         1423 c         1323 g          660 t
ORIGIN      
        1 gtcaccatcg agtacgcgcg cgccggggcg atccgcagcg tgcaggtcgc ggtggcgagt
       61 gaggaggccg gggggcggcg ggtcggacgg atccgagtgc tgcagccggc gggtgtcagt
      121 tacccgccgg acatgctgct gcacaccgac ctcgcagccg gcgcggcact cgtgcgcgcg
      181 ggcgatgagg cctggagcat gaccatgctg caggcgcggc tgttctggcg catggtgctc
      241 ggacgcgtct cgctgaagaa cctgagcggc ccgctgtcga tcgccgagtt cgccggcgat
      301 tccgccgagg cgggcatcgc ctcgttcctg agctttctgg tgctcatttc tctgtcgctc
      361 ggattcctca acctgctgcc gattccgatc ctcgatggcg gacagatcct gtttcagctg
      421 gtggagtggc tgaagggcag cccgctctcg gagcgcgcgc aggctctcgg gcagcaggtg
      481 gggatcgccc tactcatcgt cctgatgggt gtcgcgctgt acaacgatat tgcgcgccag
      541 ttcggttgag cgtgtcttgg cccgccaggc atccgcaggt cctcggcccg cggttcttaa
      601 gtcgtgcaac aggtgagatg agtcgcgccg cgggcgccgg ttgaaaggca gacggtggtc
      661 agaacatgaa ggggcgcgca gccgcgagtc cacatcgcgc tgacgtggca gggggcccgg
      721 ggagcgttgc cgcgcgcggg cagggcgtgg cgacaccgcg tgccggccac cgcagcgcac
      781 cgctcgcgct gctcgccgcg ctggcactcg cagcgcgctg cgcactcgtg cccgcacaga
      841 cccccattgc gcccgagcag agcaccagcg ccccggaacc agggccccca gcgcccacgc
      901 aggcgccgcc cgctcccgcg cagacgccgc ccgtgcccgc gaccacgccc actgcacccg
      961 agcagacgac acccgtcccc gagcagacgc cgccccggcc cgcgcccgcg gccacggtgc
     1021 cggcggactt cactgtcacg gacatcaagg tcgagggcct gcagcgcgtc tccgagggca
     1081 ccgtctacaa ctacctgccg gtgaacatcg gcgaccatct cactccgcag cgcgtgcgcg
     1141 aggcgatccg cgcgctctat gccaccggtt tcttccgcga cgtgcagctg cgccgcgatg
     1201 gcagcaccct catcgtggtg gtgctcgagc gtccctccat cgagagtttc gagatcaccg
     1261 gcaacaagga catcaagacc gaggacctgc agaagtcgct gcgcggcgtg gggctggcga
     1321 ccggcaagac cttcgaccgc tcagtgctcg aggacgtgac gcaatatctc accgatcagt
     1381 acttcgcccg cggcaagtac ggcgtgcgga tcaattccag cgtcgaggag gaggccggca
     1441 accgcgtgaa gatcaagatc gacatcaagg agggctcgcg cgccaagatc cgtcagatca
     1501 acatcgtcgg tgtcaccaag tacccgcaga aggccattct cgacaccctc gagctgaaga
     1561 cccccaactg gctctcctgg tatcggcaag acgatcgcta ctcgcgcgaa tcgctgcagg
     1621 gcgatctgga gaagatccgc aacttctaca tggaccgcgg ctacgctaac ttcaatctcg
     1681 cctcgacgca ggtggcgatc acgcccgaga aagaggacat cttcatcacc gtcaacatcg
     1741 aggagggcca ggtctacaag gtggcggaga tcaagctcgc gggcaccttc gtggtcccga
     1801 agagagagct cgagcagctg ctggtgatcc acccgggcga caccttcaac cgcaagcgca
     1861 tcaccacttc ccaggagctg atgcagaatc gtatggggcg cgatggctat gccttcgcca
     1921 aggtcgagcc cgtgcccacc gcggataacg ccaaccacac cgtggcgctc actttcttcg
     1981 tcgacccggg caaccgcgtg tacgtgcgca acatcacctt ctccggcgcc aaccgcatca
     2041 acgacgaggt gctgcgccgc gagctgcggc agctcgaggg cggctggctc tccaacactt
     2101 cgctcgagcg ctccaagcag cgcatccagc ggctgccgta cgtgaagacg gtcgatttcg
     2161 agaccacgcc ggtcgccggc gcacccgacc tggtcgatgt gaactacaag atcgaggagg
     2221 gaccggcgtc gcagctttcc ggcggcatcg gctggtcgga gacctacaag ttcatgctga
     2281 acgccagcta cgcggatgca gacttcctcg gcaccgggca gcgcgtcgcc atcaacctca
     2341 acggcggcgc cttcagcaag gtgtacagca tcagtcacac gaacccgtac accaacatcg
     2401 ataacctgca gcgcaccatc tcggtcacct acagcgacgt gacccagttc gtgtcatcgt
     2461 cctcgacctt ctcctcgcag accctgagtg tcgggcccac ctgggcgtac cccatcaccg
     2521 agttcatgta catgcgcatg ggtatcgcct tcaacagctc gcacctgctc accaactcac
     2581 tgagcagcgc cgagcaggcc cagcagtggg tgcagcagaa cggccattcc ttcagccgcc
     2641 tggcacacga cgacactacc aacaacgagt acgtgttcta cggcaccgat ttcaccacca
     2701 ccgaggcggt gatcggctgg gattgggaca cgcgcaaccg cacgctgttc gccgaccgcg
     2761 gcatgcgcca gtcgatctcc ggggcgatca ccttcccctt cagcaccgtc aagtactgga
     2821 tcggcaatta ccagttcgtc aagtacgtgc ccattgcgca tgggttcttt ttctcgttcc
     2881 tcgagggcgt cgattacggc aagccgctca gtgggaccac cgcgattccg ccctaccgca
     2941 atttcttcgg cggcggcccc gacaacgtgc gcggcttccg cgagagccgg ctggggccga
     3001 aggaccagtt cggcaacccc tacggtggca atatgcgcat caccagccag aacgagctga
     3061 tcttccccat gccctccaag tacgcgcaga ccgcgcgcgt cagcctgttc ttcgacgtcg
     3121 gcggcgtctt ctacgagggc aacacgatca agttctacgg ccccgacaac atcagccccg
     3181 tcgactacag cttccacggc tggccaagcc tgcgccgctc ggtcggcgtc gccgtgcagt
     3241 ggctggcgcc gctcgggctc ttccgattca gcttcggggt gccgctcaat gcccagcacg
     3301 gcaatggcat ccagacttgg ggggatgaga aggagggctt ccagttctca gtcggcaatg
     3361 ccttctgaac caccccgcga aattgcgccc gtggtgatcg gcgagcgctc aaccctcagc
     3421 aagatacgca atttcgcggg gaccccgtgc ggagacaaag aatcgagcat cgcgcttgcg
     3481 cgcgttctac gcgatactag gcgtttgggc cggacgcgac ttacgtccgt aatgatgcgg
     3541 gcttaacacg agggtaggta gtttccgtga atcttgcgat taagagcaga gtgggggtgt
     3601 ggatgatggc ggcgagcgcg gcactcgcgg cgttgccggc cgcggcggcc gaaccgaaga
     3661 tcggggtggt cgattttcag cgactcgcca tcgagtctcc gcagggcaag gcggtgatgg
     3721 agtcgatgcg cgccgaattt gcgccgcgcg agcgcaccct gcaggcgcag gggcaggcgg
     3781 tgaaggccaa ggaggagaag ctgcagaaag acggcgcgac catgagcgag gagcagcgcg
     3841 cgcgcgccga gaaggacctg cgcgatgggg cgcgcgactt cgagcgcgcc aagggcgagt
     3901 tccaggacga catgaacgca cgccgcaacg aggagctgtc gcgtctgcag cgtaccatcg
     3961 tcgaggaagt gcgcacctac ggcaaggcgc agggcttcga cctgatcctc gcgcaggaag
     4021 gcgtgatcta caacgcctcc gcctccgaca ttactcccgc agtcctcacc gcactgcagg
     4081 cgcgcggcgg cagcgccgcg gcggccaagc cggcggcgcc gccgcccccg gccaagccgc
     4141 cgacccaata ggcccgccgc gctcaccgcg ac
//
LOCUS       DATDMP010000469         2330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1298865, whole genome shotgun sequence.
ACCESSION   DATDMP010000469 DATDMP010000000
VERSION     DATDMP010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298865"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VI195_08175"
     CDS             <1..500
                     /locus_tag="VI195_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY6824403.1"
                     /translation="GVFAHFGSSVTDNFGRLMRFERAGVHVLVDYAHNPEGLRGLLRV
                     AEHVRAGGGRLGLLLGHAGNRQDDELAALAAAAAQFHPALIVVKENEAHLRGRPPGEV
                     PRLIQAALVRAGVSPATLQLRMSELEAVHAALAWAQPGDVLALPVHSAAARAAVVELL
                     GASGT"
     gene            complement(503..1153)
                     /locus_tag="VI195_08180"
     CDS             complement(503..1153)
                     /locus_tag="VI195_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY6824404.1"
                     /translation="MNAIVSAFLLTFAGLFPIVNPLEGAPFFLGLTAGLPDAERRVLA
                     GKAAVNGFALLLGSMALGPWLLEVFGIQLPVVRIAGGLVVAALGWKLLTQEQWSDHAA
                     GVQAESARKVGSFYPLTMPLTVGPGSMSVAITIGSRKPGFTRLSELAEHASGALLGLI
                     AIALSIYLAYRFAANIARLLGETGLEVLVRLSAFILMCIGIEIVWSGYSTLTSAAR"
     gene            complement(1319..2041)
                     /locus_tag="VI195_08185"
     CDS             complement(1319..2041)
                     /locus_tag="VI195_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6824405.1"
                     /translation="MLEPTLHSGLDTSAVRLRRTASGSAGPQTLPDLLLIERARAGES
                     SAIEALIHRYSGRLFRVAHSVLLDPQRAEDAVVEALLAAFADLSRYEPTGKFAAWLTR
                     LVYNHARTLRISSRGLQRMTPIEAATGEPSPAADALRERQQLEQSIAALPEVFRIVYV
                     LRMVEGISGIETAASLGLHETTVRTRLFRAHRRLPPDTAQRLRAAPALLELSAERAER
                     ITSAALAQLPHSSMLMISAASP"
     gene            2077..>2330
                     /locus_tag="VI195_08190"
     CDS             2077..>2330
                     /locus_tag="VI195_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824406.1"
                     /translation="MRRPVRLAAYCAACALQVWLAAIPAAGADLETGPTVHPRRWPKL
                     HPPLARDALLDARVEQLLARMTPAQKVGQLIQADIGSITP"
BASE COUNT          373 a          781 c          821 g          355 t
ORIGIN      
        1 ccggcgtgtt cgcgcacttc ggctcgagcg ttaccgacaa tttcggccgg ctgatgcgtt
       61 tcgagcgcgc cggtgtgcac gtgctggtcg attacgcaca caatccggag ggtctgcgcg
      121 ggctgctgag ggtggccgag cacgtgcgcg cgggcggcgg gaggctcggg ctgctgctcg
      181 gccacgccgg caaccgccag gatgacgagc ttgcggcgct cgctgcggcg gcggcgcagt
      241 ttcaccccgc gctcatcgtc gtcaaggaaa acgaagcgca cctgcgcggg cggccgcccg
      301 gcgaggtgcc acggctcatt caggcggctc tggtgcgtgc cggcgtgtca cctgcgactc
      361 tgcagctgcg catgagcgag ctcgaggcgg tgcacgcagc gctcgcctgg gcgcagcccg
      421 gggacgtgct ggcgctgccg gtgcactcgg cggcggcgcg cgcggcagtg gtggagttgc
      481 tcggcgcctc agggacatga gctcagcgcg ccgcgctcgt cagtgtgctg tagccgctcc
      541 agacgatctc gatgccgatg cacatgagaa tgaacgccga gagccgcacg agcacctcga
      601 gcccggtctc accgagaagc cgcgcgatgt ttgcggcgaa gcggtaggcg aggtagatgc
      661 tgagcgcaat cgcgatgaga ccgagcagcg caccgctggc gtgctcggcg agctctgaca
      721 gtcgcgtgaa gcccggcttg cggctgccga tggtgatggc caccgacatc gaccccggtc
      781 cgaccgtgag cggcatggtg agcggataga agctgcccac cttgcgcgca ctctccgcct
      841 gcactcccgc cgcatggtcc gaccactgct cctgcgtgag cagcttccag ccgagcgccg
      901 cgactacgag accgcccgcg atgcgcacca ccggcagctg gataccgaac acctcgagca
      961 gccacgggcc gagcgccatc gagccgagca gcagtgcaaa accgttgacg gcggccttgc
     1021 cggcgagcac acgccgctcg gcgtccggga ggcctgcggt caagccgagg aagaagggcg
     1081 ccccctcgag cggattgacg atggggaaaa gaccggcgaa cgtcagcagg aacgcgctga
     1141 cgatcgcatt cacttcgcgc cggctcgcaa tacgctgcgc tgcgtccctt ccatatacca
     1201 tccagcgtca ggacagcgtc gagagtatgg atgtcggcca ctgcattgtc accgggatga
     1261 gccttgcatg agccgagcat ccgtgcgccc ggtcgtgaaa gggcgagagc gtcgcctttc
     1321 agggcgaggc agcggagatc atcagcatcg agctgtgagg cagctgcgcg agcgctgcgc
     1381 tcgtgatgcg ctcggcacgc tccgccgaga gttccagcag cgccggtgca gcacgcaagc
     1441 gttgcgcggt gtcgggcggt aaccgccggt gcgcgcggaa cagccgcgtg cgtaccgtag
     1501 tctcgtgcag tccgagagat gcggcggtct cgatgccgct gatgccctcc accatgcgca
     1561 gcacgtatac gatgcggaac acctccggca gagcggcgat cgactgctcg agctgctgcc
     1621 gttcgcgcag cgcatctgcg gccggcgaag gctcgccggt tgccgcttcg atcggtgtca
     1681 tgcgctgcag gccgcggctg ctgatgcgca gcgtgcgcgc gtggttatag acgaggcgcg
     1741 tcagccacgc ggcgaacttt ccggtcggct cgtagcggct gagatccgca aaggcggcga
     1801 gcaacgcctc gaccaccgcg tcctcggcac gctgcgggtc gagcagcaca ctgtgcgcga
     1861 cccggaagag tcggccgctg taacggtgta tcaatgcctc gatcgcagag ctctcgcccg
     1921 cgcgcgcgcg ctcgatgagc agcagatccg gcagtgtctg cggcccggcg ctccccgaag
     1981 cagtgcgccg caaacgtacg gcactcgtgt cgaggccgct gtgaagggtc ggttccagca
     2041 tgtgcatcac cctctgctgt gtatcctagg agccccatga ggagaccggt tcggctggcc
     2101 gcgtattgcg cggcctgcgc gctgcaggtg tggctcgccg ccattccggc ggcaggcgcg
     2161 gacctggaga cgggccccac cgttcatccg cggcgctggc cgaagctgca cccgccgctc
     2221 gcacgcgatg cgctgctcga tgcccgcgtg gagcagctgc tcgcacgcat gaccccggca
     2281 cagaaggtgg gtcagctgat ccaggccgat atcggcagca tcactccgga
//
LOCUS       DATDMP010000470         5837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2188012, whole genome shotgun sequence.
ACCESSION   DATDMP010000470 DATDMP010000000
VERSION     DATDMP010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188012"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VI195_08195"
     CDS             complement(<1..867)
                     /locus_tag="VI195_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEY6824407.1"
                     /translation="MAEPLPLCYLNGEFLPLEEARISPLDRGFLYADAVYEVMPVYGG
                     RPFRFAAHTERLARSLAGIGMEDPHTREEWRAILGSLIERNGGGDCYLYWQVTRGAQL
                     GRTHAPLPRIPRTVFAFCAPWPQPPADLSAKGVSCVTAADTRWARCDIKSTALLANVL
                     LRQLSVDATAAETILLRDGELTEASASAVHVVLGGELLMPPNSRRILPSTTRGVVEEL
                     AGRAAIACRVQVISETQLRAADEVWISAATREVQPVTSLDGHPIGSGRPGPLWRRVYE
                     ELQRYKQELAGTP"
     gene            complement(878..2074)
                     /locus_tag="VI195_08200"
     CDS             complement(878..2074)
                     /locus_tag="VI195_08200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY6824408.1"
                     /translation="MIRSLPTACALALFVSKAYATSPAPAPAAPPLAAPSAPIAAPVP
                     AAPVVTARSWILVDHFSGRVLAQQRAEERAEPASLTKLMTAYVVFAALADGRLKLTDT
                     AAISEHAWRAEGSRTFLQVGTRVPVDILLQGMIVQSGNDATIALAEQVGGTEPAFAQM
                     MNEYAHRLGLTATHYVNSDGLPSPEHYTSARDVATLANALIRDFPQYYPLFSVREFRW
                     NNIRQGNRNTLLGKDPSVDGLKTGHTDTAGYCLAASASRNGMRLVSVVMGAANEKARE
                     EATAALLGYGYTFYETVRLKSANETVLKPHIYKAANEFAAIGVPHDVYATVARGQAGA
                     VKTRALLSRDPLIAPLTAGQNVGELTVADGSGAVIERVPLVALSAVPQGGLWTRAWDS
                     VSLMFH"
     gene            complement(2201..3028)
                     /locus_tag="VI195_08205"
     CDS             complement(2201..3028)
                     /locus_tag="VI195_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY6824409.1"
                     /translation="MTRFAGRAYALAAVASALLVLSACSITAQRPPPAASHAGPPAAP
                     APPPAVDALPDAVPRAEPRSAHGNPPFYEVLGRRYYVLTTADGYLERGVASWYGPTFH
                     GGNTSSGEPYDMYAMTAAHKTLPLPCYARVTNLRNGRSVVVRINDRGPFAANRLIDLS
                     YSAAMKLDMVRDGTTLVEVRALTATVPDVLTRSSAQPPPALYVQAGAFADQQHAQRLL
                     ARLQAAGLGRAFIALPLQNGAHLYRVRLGPVESVAQFDELTARLAALGVPDARLAIE"
     gene            complement(3025..4038)
                     /gene="mltB"
                     /locus_tag="VI195_08210"
     CDS             complement(3025..4038)
                     /gene="mltB"
                     /locus_tag="VI195_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY6824410.1"
                     /translation="MPLLALLACLPALAQPASPPALATSFDLQRPEISAFIDEVAQRD
                     GLKRKDVRRLLKKARPQPKIIDIMNRPIEKVAPWWEYRDHFVNAERVSDGAEFWADHR
                     AVLERVTATYQVPPEYLVAILGVETRYGRTTGHYRVLDALATLAFDYPPRHNYFAGEL
                     AQFLVLVKENRLDPLTTTGSYAGAMGAPQFMPSAYRRYAVDASNDQRRDLWGNWDDIL
                     ASVANYLRENGWTAGGPVLAETRIDPDANFHLEPHGLELNDTVDSLGEHGVKVDLDVP
                     ADTPALLVLAEQPDGPAYRVGFHNFYVLTRYNTSTRYAMAVHDLAQAIAQRMQGATAA
                     TVP"
     gene            complement(4054..5193)
                     /gene="rodA"
                     /locus_tag="VI195_08215"
     CDS             complement(4054..5193)
                     /gene="rodA"
                     /locus_tag="VI195_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY6824411.1"
                     /translation="MIYEEITQGSRTRRTLSGAARVLLELRIDGPLVMGLSLIAVYGL
                     VVLYSASGQSVPTVLRTVARLVLGTIAMLLLARVNPNFLRRSTPWVYAAGCLMLLIVA
                     AIGHVGMGAQRWLNLGLFRFQPSELMKLAVPMMCAWYLHERPLPPSLAALAAMGALIL
                     VPVALVAMQPDLGTAALIAVAGALVIVLAGLPMRVMAGLLTLAAAAAWFGWSFMHDYQ
                     RKRVLTFLDPQNDPLGAGYHIIQSQIAIGSGGVFGKGWMNGSQAQLEFLPERSTDFIF
                     AVIGEEFGLLGLLLLLLLYAFVVGRAIYLATQTQDTFARLLAGSLALTFFVYVFINAG
                     MVTGLLPVVGVPLPLVSYGGSSVVTVLAGFGILMALYSRRKLIGS"
     gene            complement(5190..>5837)
                     /locus_tag="VI195_08220"
     CDS             complement(5190..>5837)
                     /locus_tag="VI195_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY6824412.1"
                     /translation="GAPTGIDISGEKAGILPSREWKEKTFARPVDRTWFPGETVSFGI
                     GQGYLTVTPIELAHYASIVATRGKVWRPRLVTAYRDPRSGELHRIAPVSDGEVQGVAA
                     DDWQRVVHGMIGVTQRGTAAAIGLHAPYTFAGKTGTAQVFTVARTEKYNASKINERLR
                     DHSWFIAFAPADAPRIALAVLEENGGAGASAAAPIARKVLDAYLLDADGKVKPSS"
BASE COUNT          969 a         1939 c         2057 g          872 t
ORIGIN      
        1 gggggtaccg gcgagctcct gcttgtagcg ctggagctcc tcgtacaccc gccgccacag
       61 cggcccgggg cgaccactgc cgatcggatg accatcgagc gaggtcacgg gctgaacttc
      121 gcgcgtcgcg gccgaaatcc acacctcatc ggccgcacgc agttgcgtct cactgatcac
      181 ctggacgcgg caggcaatcg ccgcgcgccc ggcgagctcc tcgaccacac cgcgcgtggt
      241 gctgggcagg atgcgccgcg agttgggtgg catcagcagc tcgccaccga gtaccacgtg
      301 cacggcggat gccgaggctt cggtcagctc gccatcgcgc agcaggatgg tttcggcggc
      361 cgtggcgtcc accgacagct gccgcaacag cacgttggcc agcagcgcgg tcgacttgat
      421 gtcgcaacgc gcccagcggg tatcggccgc ggtcacgcag ctgacaccct tcgcgctgag
      481 gtccgcaggc ggctgcggcc agggcgcgca gaaggcgaac acggtgcgcg ggatgcgcgg
      541 cagcggcgca tgggtgcggc cgagctgcgc gccacgcgtc acctgccagt agaggtaaca
      601 gtcgccgccg ccgttgcgct cgatcaggga gccgagtatc gcgcgccact cctcacgcgt
      661 gtgtggatcc tccatgccga tgccggcgag actgcgtgcc aggcgctcgg tgtgcgccgc
      721 gaagcggaac gggcggccgc cgtacaccgg catgacctcg tagaccgcat cggcgtaaag
      781 gaaaccacga tcgagcgggg agatgcgtgc ctcctcgagc ggcaggaact caccgttcag
      841 gtagcacagc ggcaggggct cggccacgct cgtgccctca gtggaacatc agcgagacac
      901 tgtcccaggc ccgcgtccac aggccgccct ggggcaccgc gctcagcgcg acgagcggga
      961 cgcgctcgat caccgcgccg ctgccatccg ccacggtgag ctcgcccacg ttctggcccg
     1021 cggtgagtgg cgcgatcagc gggtcgcggc tgagcagcgc gcgcgtcttc accgcacccg
     1081 cttggccgcg cgccaccgtc gcgtaaacat cgtgcggcac cccgatggcg gcgaattcat
     1141 tcgccgcctt gtagatgtgg ggcttcaaca ccgtctcgtt ggcgctcttc aggcgtacgg
     1201 tctcgtaaaa ggtgtaccca tagccgagca gtgccgcagt cgcttcctca cgcgccttct
     1261 cgttcgcggc gcccatcacc accgacacga gccgcatgcc gttgcggctg gcggaggcgg
     1321 ccagacaata gccggcggta tcggtgtgcc cggtcttcag gccatcgacc gagggatcct
     1381 tgccgaggag ggtgttgcgg ttgccctggc ggatgttgtt ccagcggaac tcgcgcacgc
     1441 tgaagagcgg atagtactgc gggaagtcgc ggatcaacgc gttggcgagc gtcgccacat
     1501 cccgggcgct ggtgtaatgc tcgggagagg gcaagccgtc gctgttgacg tagtgcgtcg
     1561 cggtgagtcc gagccgatgg gcatactcgt tcatcatctg cgcgaacgcc ggctcggtgc
     1621 caccgacctg ctcggcgagc gcaatggtcg cgtcgttgcc cgactgtacg atcatgccct
     1681 ggagcaggat gtccaccggc acgcgcgtac ccacctgcag gaaggtgcgt gagccctcgg
     1741 cgcgccaggc gtgctcgctg atcgcggccg tgtcggtgag cttgagcctg ccgtcggcga
     1801 gcgccgcgaa taccacgtac gcggtcatga gcttggtgag gctcgcgggc tccgcgcgct
     1861 cctccgcacg ctgctgcgcc agcacccggc cgctgaaatg atccacgagg atccaggagc
     1921 gcgccgtcac cacaggcgcc gcgggaaccg gcgccgcgat gggcgcggaa ggtgcggcca
     1981 gcgggggtgc tgcgggggcg ggtgcgggtg aggtggcgta agccttcgag acaaagagcg
     2041 ccagggcgca ggcggtgggc agtgagcgga tcacggttgt aggtttccct aggtcaggaa
     2101 aaaatcgaat cttagccggg gtccccaaaa aatcacgtgt cttgtcgcac agcgtgactc
     2161 tcgccacgcc cgtcgaacgg gattttttga ggtgggttcc tcactcgatg gcgagccgcg
     2221 catccgggac gccaagcgcg gcgagccgcg cggtgagctc atcgaactgt gcgacgcttt
     2281 cgaccggacc caggcgcacc cggtagaggt gcgcgccatt ctgcagcggc aaagcgatga
     2341 acgcgcgccc gagcccggcg gcctgcagcc gcgcgagcag ccgctgcgca tgctgctgat
     2401 cggcgaacgc gccagcctgc acatagagcg ccggcggcgg ctgcgcgctc gagcgcgtga
     2461 gcacatcggg aaccgtcgcg gtgagggccc gtacctccac cagggtcgtg ccatcgcgaa
     2521 ccatgtcgag cttcatggcc gcgctgtagg aaagatcgat gagccgattg gcggcgaagg
     2581 ggccgcggtc attgatgcgc accaccacgc tgcggccatt gcgcagattc gtcacccgcg
     2641 cgtagcaggg caacggcaga gtcttgtggg cggcggtcat ggcgtacatg tcgtagggct
     2701 caccgctcga ggtgttgccg ccgtggaagg tgggaccgta ccaggacgcc acgccgcgct
     2761 cgaggtagcc atcggccgtg gtgagcacgt aatagcggcg gccgagcacc tcgtagaagg
     2821 gcggatttcc gtgagcgctg cgcggctcgg cgcgtggcac cgcatcggga agggcatcga
     2881 cggcgggtgg cggtgcgggc gccgccggcg gccctgcgtg gctggctgcg ggcggcgggc
     2941 gctgcgcggt gatcgagcag gcgctgagca cgagcagcgc ggatgccacg gcggcgaggg
     3001 cataggcgcg gcccgcgaag cgcgtcatgg caccgtggcg gcggttgcgc cctgcatccg
     3061 ctgcgcgatc gcctgggcga gatcatgaac cgccatggcg tagcgcgtgc tcgtgttgta
     3121 gcgggtgagc acgtagaaat tgtgaaaccc gacacggtag gccgggccgt cgggttgctc
     3181 ggcgagcacc agcaatgccg gtgtgtcggc cggcacgtcc aggtcgacct tcaccccgtg
     3241 ttcgccgagc gagtcgacgg tatcgttgag ctcgagaccg tgcggctcga ggtggaaatt
     3301 cgcatccgga tcgatgcgcg tctccgccag caccggaccc cccgcagtcc agccgttctc
     3361 gcgcagatag ttggcgacgc tcgccaggat atcgtcccag ttgccccaca ggtcgcgccg
     3421 ctgatcgttg ctcgcatcga ccgcatagcg ccggtaggcg gagggcatga actgcggtgc
     3481 gcccatggcg cccgcgtagg agccggtggt ggtgagcgga tcgagccgat tctccttcac
     3541 cagcacgagg aactgcgcga gctcgcccgc aaagtagttg tggcgcgggg ggtagtcgaa
     3601 ggcgagcgtc gcgagcgcat cgagcacccg ataatgcccg gtggtgcgtc cgtagcgcgt
     3661 ctcgacgccg aggatggcga ccaggtactc cggcggcacc tggtaggtcg ccgtaacgcg
     3721 ctcgagcacc gcgcgatgat cggcccagaa ctccgcgcca tcgctcacgc gctcggcgtt
     3781 gacgaagtgg tcgcggtatt cccaccacgg cgccaccttc tcgatcggcc ggttcatgat
     3841 gtcgatgatc ttcggctgcg gccgtgcctt cttgagcagc cgccgcacgt ccttgcgctt
     3901 gagaccgtcg cgctgcgcga cctcgtcgat gaaggccgag atttccgggc gctgcaggtc
     3961 gaagctcgtc gcgagcgccg gcggactcgc cggctgggcg agcgccggca ggcaggcgag
     4021 caatgctagg agtggcaagc ggcagcggtt caactacgac ccgatgagtt tgcggcgcga
     4081 gtacagcgcc atgagaatac cgaagcccgc cagcaccgtc acgaccgagc tcccgccgta
     4141 actgacgagc ggcagcggca cgccgaccac cggcaggagt ccggtcacca tgccggcatt
     4201 gatgaacaca taaacgaaga aagtgagcgc caggctgccg gcgagcagcc gcgcgaacgt
     4261 gtcctgggtc tgcgtcgcca ggtagatggc gcgcccgacg acgaacgcat acagcagcag
     4321 cagcagcagc aggccgagca gcccgaactc ctcgccgatc accgcgaaga tgaaatcggt
     4381 cgagcgttcg ggcaggaatt cgagctgcgc ctgactgccg ttcatccagc ccttgccgaa
     4441 cacgccgccc gagccgatgg cgatctgcga ttggatgatg tgatagccgg caccgagcgg
     4501 atcgttctgc gggtcgagga aggtgagcac gcgcttgcgc tggtagtcgt gcatgaagct
     4561 ccagccgaac caggccgccg ccgccgcgag cgtcagcagt cccgccatca cccgcatcgg
     4621 cagcccggcg agcacgatca cgagcgcgcc cgcgaccgca atcagcgccg ccgtgcccag
     4681 atccggctgc atcgcgacca gcgccaccgg caccaggatg agcgcgccca tcgctgcgag
     4741 tgctgccagc gagggcggca gcggccgctc gtgcagatac caggcgcaca tcatcggcac
     4801 ggccagcttc atgagctcgg atggctggaa gcggaacaga ccgaggttca gccaacgttg
     4861 cgcacccatg ccgacgtggc cgatcgcggc aacgatgagc agcatcaggc aaccggcggc
     4921 atacacccag ggtgtggagc ggcgcaggaa attgggattg acgcgtgcga gcagcagcat
     4981 ggcgatcgtg ccgagcacca ggcgcgcgac cgtgcgcagc acggtcggca cgctctgacc
     5041 ggaggcgctg tagagcacca cgaggccgta gacggcgatc agcgacagtc ccatcaccag
     5101 cgggccgtcg atgcgcagct cgagcagcac gcgcgccgcg ccgctgaggg tgcgccgggt
     5161 acgcgagccc tgggtgattt cctcgtagat catgaggagg gcttcacctt gccgtccgca
     5221 tcgagcaggt aggcgtcgag caccttgcgc gcgatcgggg ctgcggcgct cgcacccgcg
     5281 ccgccgttct cctccagcac cgcgagcgcg atacgcggtg catccgcggg tgcaaaggca
     5341 atgaaccagg aatgatcgcg cagccgctca ttgatcttgc tggcgttgta tttctcggtg
     5401 cgcgccaccg tgaacacctg tgcggtaccg gtcttgccgg cgaaggtgta gggcgcgtgc
     5461 agaccgatcg cggccgcggt gccgcgctgg gtgacgccga tcatgccgtg aacgacacgc
     5521 tgccagtcat cggcggcgac gccctgcacc tcgccgtcgg agaccggcgc gatgcgatgc
     5581 agctcgccgc tgcgcggatc ccggtaggcg gtcaccagac gcggccgcca gaccttgccg
     5641 cgcgtcgcga cgatactggc gtagtgggcg agctcgatcg gcgtcacggt gaggtagccc
     5701 tgcccgatgc caaagctcac cgtctcgccc gggaaccacg tgcggtcgac cggccgtgcg
     5761 aaggtcttct ccttccactc acgcgagggc aggatgccgg ccttctcgcc actgatatcg
     5821 atgccggtcg gcgcccc
//
LOCUS       DATDMP010000471         4603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_964634, whole genome shotgun sequence.
ACCESSION   DATDMP010000471 DATDMP010000000
VERSION     DATDMP010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964634"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..469
                     /locus_tag="VI195_08225"
     CDS             191..469
                     /locus_tag="VI195_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824413.1"
                     /translation="MALADAALEERAPCPVSSQEQARGLAEVLFQQGDFQHSGACYQA
                     AGEYALANRAFLKAVEPQSAKTAQQLSDQGEQAKALAHKLQRAFGVEH"
     gene            complement(496..1182)
                     /locus_tag="VI195_08230"
     CDS             complement(496..1182)
                     /locus_tag="VI195_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824414.1"
                     /translation="MSLLLGRLVLTLRATCCALSRISMSTGKSTAGTRVRCTSAARIG
                     LLLGVNTFLTPAAGADFADVLAARAPLAGYFRDARGAATRVTEQARALNAQHGLVAPP
                     GPGVGAAAAPPLSDFPARLHHAFGSGLVLPSEAALQDDREALLRNSDAFVRAAVRVTL
                     GKERRGFVYVDMGAADSALKWQGLAGIHSGRDVDLLGGWRHVTYHFSPGRGFDSIDFN
                     GPFLGATLAW"
     gene            complement(1267..1680)
                     /locus_tag="VI195_08235"
     CDS             complement(1267..1680)
                     /locus_tag="VI195_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6824415.1"
                     /translation="MKTIPIGAQGQYAHRVEPAHLANRFKDSILPPVFSTPYLILIME
                     NAALNAVKQYLEPGESAVGTRVDVRHLVATPVGREVVGHAEVTGTEGRKLFFRVWATD
                     GKEEIGSGIHERTVVNVSRIVERMTAKYGPASRAD"
     gene            complement(1714..2430)
                     /locus_tag="VI195_08240"
     CDS             complement(1714..2430)
                     /locus_tag="VI195_08240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6824416.1"
                     /translation="MSFIIPAYNEARLLGNTLSAIEKAARALRLTFEVIVVDDASVDR
                     TSEVARAHGARVVPVHFRQIAATRNAGARAAAGEWLVFVDADTIASEVVLRDAIAALT
                     AGAVGGGCVVHFDGRVPRYGRMLIALLLPLYRSLGLAAGCFLFCTRQAFDAVGGFDER
                     LFAAEEVVMSRALRRLGRFVILRAAVVTSARKLRAHSAGEVLGTLLRLMLSGSRGVRR
                     RDGLELWYGQRRRDPEAPDS"
     gene            2910..4130
                     /locus_tag="VI195_08245"
     CDS             2910..4130
                     /locus_tag="VI195_08245"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY6824417.1"
                     /translation="MDAVKQPLKDRPAASAHGKWALFGYGFRPFFLASGLAGLLLIPW
                     WAASLAWGVALGTSWPPNLWHGHEMLFGFIVAAIAGFLLTAVPSWTGARGFAGWPLAT
                     LAALWVLGRVGVATSAFWPAWLVAALDLSFLPALAGCVLPALVRARNRNTPLLVVLAT
                     LWLTNVAFYWGLAHDDPQLARRALLLGIDVVLLLVTVIGGRIIPAFTTSAFKQHGVSS
                     ALRVWRGMTPLAILVMLAVAIIDLSRPEGAAAGWVALVAALIQAVRLAQWRGLRTLRM
                     PIVWVLHLAYLWLPLGLALKALARLAGLGVAAYYLHALAIGAAATMIMGVMTRASLGH
                     TGRPLTVTRSIACAYVLLTAAAAVRVFGATWIALPYDLIIVLAAALWTAAFALFLWVY
                     GPMLLQPRADGKPG"
     gene            complement(4167..4478)
                     /locus_tag="VI195_08250"
     CDS             complement(4167..4478)
                     /locus_tag="VI195_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6824418.1"
                     /translation="MRTAVSIAVTVVMFAGGLPTAAAADLQAGRQLSQASCAACHGVN
                     GIGIIALYPNLAGQKNEYLVAQLRAFREGNRTNPIMSPMATHLSDVDIENLAAYYSSL
                     R"
BASE COUNT          817 a         1485 c         1468 g          833 t
ORIGIN      
        1 aggctatgca tctcgtaatg gggcggtgac ttatggaatt tcttgtcatt gatatcggtg
       61 ccacggtcgt tgcagcactt agctacctgg cggttgtggt ttgggatgac gtcacgggtg
      121 aaccgtaatg tttgtgcact cgcagacttt gcgcgtcgtc gcagccgcgt tatttctggc
      181 ggccacaccc ttggcgctcg cggacgcggc gctcgaggag cgcgcgcctt gccccgtgtc
      241 gagccaggag caggcgcgag ggcttgccga ggtgctcttc cagcaaggcg actttcagca
      301 ctcaggtgcg tgctaccagg cggcgggaga atacgcgctg gcaaaccgag ccttcctgaa
      361 ggcggttgag ccacagagcg caaaaactgc ccaacagctc tccgatcagg gggaacaggc
      421 gaaggcgctc gcgcacaagc tgcagcgcgc attcggagtt gagcactgaa agcactacgg
      481 tgcgcgggcg cctcgctacc aggcgagggt cgcgccgagg aagggaccgt taaaatcgat
      541 cgagtcgaat cccctgccgg ggctgaaatg gtaggtgacg tggcgccagc cgcccagcag
      601 gtccacgtcg cggccgctat ggatgccggc cagcccctgc cacttcagcg ctgagtctgc
      661 ggcgcccata tccacgtata caaagccgcg ccgctccttg cccagagtca cgcggaccgc
      721 cgcgcggacg aaggcatccg agtttctcaa taaggcctcg cgatcatctt ggagcgccgc
      781 ttcgctcggg agaaccaggc cgctgccgaa ggcgtggtga agcctggcgg gaaagtccga
      841 taggggtggt gcggcagcag cgccgacacc cggcccgggc ggagcgacca ggccgtgttg
      901 agcgttcagc gcacgtgcct gctcagtgac gcgggtggct gcgccgcgag cgtcgcggaa
      961 gtagccggcg aggggggcgc gggctgcaag aacatccgcg aagtcggcac cggccgctgg
     1021 tgtgagaaaa gtattgacgc ccagcagcag gccgatgcgc gctgcggacg tgcagcgtac
     1081 tctcgtaccg gctgttgact tgcccgtcga cattgagatc cgactcagcg cgcaacatgt
     1141 ggcacgcagt gtcagcacca gccgcccgag cagcaaactc aatgtctgaa gagcacatca
     1201 ggaaaactga acttgagttg acggcacgtt ttcccgaccg cgacgccgca accacgagca
     1261 cgaggatcaa tctgctcgag aggctggccc gtacttcgcc gtcatgcgct cgactatgcg
     1321 cgagacgttg acgaccgtac gctcatgaat tccgctgccg atctcttcct tgccgtccgt
     1381 cgcccacacg cggaagaaga gcttgcggcc ttcggtgccc gtgacttccg cgtggccgac
     1441 gacctcgcgc ccgacaggag tcgccacgag atggcgtacg tcgacgcgtg tgccgactgc
     1501 gctttctccc ggctcgaggt attgcttaac ggcattcaag gcggcgttct ccatgatgag
     1561 gatcaggtac ggcgtcgaga acaccggcgg caggatactg tccttgaacc gattcgccag
     1621 atgtgcaggc tcgacgcgat gcgcatactg gccctgagcg ccgatcggaa tcgttttcat
     1681 gactgcctct cgagcggtcc cggccgtttc cgctcagcta tcgggagcct cgggatctcg
     1741 tcgccgctgg ccataccaga gctccaatcc gtcgcgccga cgcacacctc ttgaaccgct
     1801 gagcatgagc cgcaacagag tacccaggac ctcacctgca gaatgggctc gcagcttgcg
     1861 tgccgaagtc accaccgccg cgcgcaggat cacgaaccgg ccgaggcgac gcagtgcccg
     1921 gctcatcacc acctcttccg cggcgaatag ccgctcatcg aagccgccaa ctgcgtcaaa
     1981 tgcctggcgc gtacagaata gaaaacaccc ggccgccaag ccaagtgatc gatatagcgg
     2041 cagcagcagc gcgataagca tgcgaccgta gcgcggcacg cgtccgtcga agtggacgac
     2101 gcagccaccg ccgactgcac ccgcagtcaa cgcggcgata gcgtcgcgca gcactacttc
     2161 gctggcgatg gtgtcagcgt cgacgaacac gagccactcg ccggctgccg cgcgtgcgcc
     2221 cgcgttacgc gtcgccgcaa tctggcggaa gtgcaccggg acgacacgtg ccccgtgtgc
     2281 gcgggcgacc tcggaggtgc gatcgacgga agcgtcatcg accacgatga cctcgaaggt
     2341 caggcgaagc gcgcgcgcgg ccttctcgat cgcgctcagg gtatttccca acagccgagc
     2401 ttcgttatag gccgggatga tgaaagacac ggaacgcatc gagctttcga aacctgcagc
     2461 ggctacagca agactgtatc gcacctttgc gtcatccgcc gtgcgagtca tccgggcgct
     2521 gctgtccgag ggaaaaaaga aggcggatca ctccggcggg gtagccggga agtgctccgc
     2581 ccaaggggga gtcagctcag aatgaatctc gcctttgcaa gtagagcaga ggtaaccaac
     2641 agcgcgattc aaccccagtc ggccgccgcg gcgataagga aataggcacg aagaggaatc
     2701 ccgctgcgac cgccagatga gttggcgagc gacatgttcg cccgggagct cggcgcgaac
     2761 atcggggtgg ttccctaaat caagcgtggc acttccctag attgcgttgc gcatcgcgtt
     2821 ccggatcagg ccatggcact tcggttctgc tgggaacgtc atgcggatgt tccgagtcag
     2881 ctatagtgcc cgcattgcga gctgcgccga tggacgctgt caaacaaccg ctgaaagatc
     2941 ggccggcggc cagcgcacat ggcaagtggg cgctcttcgg ctatggattt cggccgttct
     3001 tcctcgcctc tggactggca gggctgctgc tcattccgtg gtgggccgca tcacttgcgt
     3061 ggggcgttgc cctgggcacc agttggccac cgaatctatg gcacggccac gagatgctgt
     3121 tcgggttcat cgtagccgct atcgcaggtt tcctgctcac ggcagttccg agctggacgg
     3181 gggcacgggg gtttgccggc tggccactgg caacgctggc ggcactctgg gtgctcgggc
     3241 gggtcggcgt cgccacctcc gctttctggc cggcatggtt ggtcgctgcg ctcgacctct
     3301 cgttcctgcc ggcgttggct ggatgcgtgc tgccggccct ggtgcgggcc cgcaatcgca
     3361 acacgccgct gctcgtggtg ctggcgactc tctggctgac aaacgtagcg ttctactggg
     3421 gtctcgccca cgacgatccg cagcttgcgc gtcgcgcact cctgctcggc atcgatgtcg
     3481 tgttgctgct cgttacggtg atcggcggcc gcatcattcc cgccttcacc acctcggcgt
     3541 tcaagcagca cggcgtatcg agtgcgctgc gcgtgtggcg cggcatgacc ccgctggcaa
     3601 tcctggtcat gctcgcagtc gccatcatcg acctgtcgag acccgagggc gcggccgcgg
     3661 gctgggtggc gctcgtcgcc gccctgatcc aggcggtgag actggctcag tggcgaggac
     3721 tgcgcaccct gcggatgccg atcgtatggg ttctgcatct cgcctatctg tggcttcccc
     3781 tggggctcgc gctcaaggcg ctcgcgcggc ttgcgggact cggagttgcg gcctattacc
     3841 tgcacgctct cgccatcggc gccgcagcca ccatgatcat gggcgtgatg actcgcgcct
     3901 cgctcggcca caccggccgg ccgctcacag tcacccgatc catcgcctgc gcttatgtcc
     3961 tcctcacagc cgccgctgcg gtgcgcgtgt tcggtgcgac gtggatcgct ttaccgtacg
     4021 acctcatcat tgtgctcgct gcagccttgt ggaccgccgc atttgcactt ttcctctggg
     4081 tctacgggcc gatgctgctc cagccgcgcg cggacgggaa acccggctga gtgcaacctg
     4141 ccctgccacc tggcgccctg gcgagctcac cgcagagacg agtaataggc cgccaggttc
     4201 tcgatgtcca catcggacag gtgtgtggcc atgggactca tgatgggatt cgtgcggttg
     4261 ccttcgcgga aggcgcgtaa ttgcgcgacg aggtattcgt tcttctgacc ggccaggttg
     4321 gggtacaacg cgatgattcc gataccgttc actccatgac atgccgcaca gcttgcctgc
     4381 gaaagctgtc tgccggcctg cagatctgca gccgcggcag tcggtaagcc cccggcaaac
     4441 atcaccacag tcacggcgat cgatacagct gttctcatgg tcgctctcca ggtgacgcca
     4501 gcgcgttcgg ccgatacaac gctgcccaca gcgcaagtac cgcgacgatc agtccgatcc
     4561 agaacacact ccagaacgaa gccagcggaa atcgctcgac tcg
//
LOCUS       DATDMP010000472         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_410150, whole genome shotgun sequence.
ACCESSION   DATDMP010000472 DATDMP010000000
VERSION     DATDMP010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410150"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1208..1342
                     /locus_tag="VI195_08255"
                     /pseudo
     CDS             1208..1342
                     /locus_tag="VI195_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
     gene            complement(1577..2644)
                     /gene="lptG"
                     /locus_tag="VI195_08260"
     CDS             complement(1577..2644)
                     /gene="lptG"
                     /locus_tag="VI195_08260"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY6824419.1"
                     /translation="MSVLDRYIVRTILGTVLMVIVVLLVLGALFVFINQQKDIGVGHY
                     SALGALWYSLLNLPQLAYELMPITALIGSLLGLGSLARGSELTVIRAAGISVVRLARI
                     ALLAGLLLVLFEVLLGESLAPPLQATARAYKAFSKLSNVSFGGDGGAWVRDRDLILNV
                     AGQTSERQFGGMQIFELSPPHRLIALGHAAHAAAEGRGRWLLSDYRESRFDADSVTTQ
                     APEQRELESNVSAGFLGLAVEDPKQLTGRALWRLIQYFQSNSLDAREYVFAFWSRIAR
                     TVGVMFAVLVAIPFVLGSMRSAGAGSRMMLGLLIGLGFFLLQRLIESGTVVFQLNPVL
                     LAWIPTALLALVTSILLARTR"
     gene            complement(2641..2952)
                     /locus_tag="VI195_08265"
     CDS             complement(2641..2952)
                     /locus_tag="VI195_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY6824420.1"
                     /translation="MRFGSILGRFVLREVVLAGLVVSGVLLIMLLANEVLGRGIEVRP
                     SARLPHSRDRAERAELHWHISMPVTCLLLALHAVPLSKLRPRQGGPGLITRLRERMRG
                     L"
     gene            complement(3376..>4172)
                     /locus_tag="VI195_08270"
     CDS             complement(3376..>4172)
                     /locus_tag="VI195_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824421.1"
                     /translation="AWDMARRGGPAADAAGYICVMAGNGAFETTLDANGFPSAQDYGN
                     SYLKISTAGQQLAVVDYFAMWNAVSESVADLDLGASGPIILPDLTDASGNVKHLAAGA
                     GKDGNIYIVDRDSMSKFNSSRNNIWQEVDGALSQLARSTGAYFNGRLYLADVAPLKAF
                     SITDARLSTSPTSVSVCLGYPGGPPVISANGNSNGIVWAIDHASSAILHAFDATNLAV
                     ELYNSTQAANKRDQFGAGVKFTVPTVVDGKVFVGSKTGVGVFGLLH"
BASE COUNT          816 a         1379 c         1274 g          703 t
ORIGIN      
        1 ctgcttcgcc gtgcccaaga attgcggtta ctcagcttct gagccacacg gaagcccgac
       61 tctgcgcgca tccagccctg agaccgagcg caagcagaag taagaatcac agcctgtgat
      121 ttgtggcggc gtgagccgaa agatactatg tctgcgacgc gctctcctaa ggcttaatta
      181 gccatttgcg cttaaattag cctcaaagaa catacttaac tggagcttga gtttgggagt
      241 tgagcgtacc cacgccttcc agcagtcgtc ggttgcggcc gcagcaccgg acttgacgcg
      301 gacagaaggg gacgtgccgc gctcgggtcg cggtctcgcg ggagcgctca ggcacctgcg
      361 cattacagcg cgcagtcgtt tcggtcgatg gatcgccgcg tgccgcaagt cgcgcagtct
      421 gtgccccgag ggggcgctgg cgtctgagcg catcacgagc gtgctggtct gccgtgtgaa
      481 tgcccgcatg ggcaatgcgc tctttctcac accgctcatc cagcagttgc acgagctgct
      541 gcctgaggca accatcgacg tcgccagctc ttacccgcac gcgcccgatc tgctgggcca
      601 cttgcccggc gtgcgaaccg tgatcctgtt cccctacaag ggcgtccaaa tcgtgtggcg
      661 ctgcctcgcc gcgctcgctc gcgtacgcag gcaccgctat gacctggtca tcgaccccgt
      721 gccgaactcc acgagctccc gcatcgtgct gtggttgtgc cgcgcacggc tgcggctggg
      781 attcgcctcc gagcaccagt gggcgccgct cacccactcc gtgccggagg cactcacgcc
      841 cgcacaccgc gcgattcagc cggtcttcct gctgcactgg gcgctcggcg tcgcacacgc
      901 gcctgcccgc ttgcagctct caaccggctg tcgcgcgcgt gaacaggcgc aggctcacgc
      961 cctcgttcgc cgcgcgctgg aactgcaggg caatgatccg agccgcgtcc agcccgtggg
     1021 cttcttcgcc tatgccaccg ggtcgaagcg tttcgggcgg gagtactggt tgaagttctg
     1081 gacgcgcttc ttgcagctcg cacccgaggc agtgccggtg gaattcctgc cgacgcccca
     1141 taccactccc acgagtccga gctttgctgc gctgcacgtg ccgaccccgc ggcgcctggc
     1201 cgcggcgatg ggccaggtgt gtatgtttat ctccgccgac accgggccga tgcacctcgc
     1261 gagctccacc gcggtgccga cggtggggct gttttgcgcc tccaacccgg cgctctacgg
     1321 accgctcaag cctagcgatc tggcactcga tgccacccat cactcgccgg ccctgctcgc
     1381 gcaactgtgc taccagcact ggcggcgctg ctgcgccctg gcgaacatga cggcacagga
     1441 cctcggcggt actgccaaca ggagcgacca gcccccgggg gcgccccccg ccaagggctc
     1501 ccccacgaaa ttttcctcgc cacgcctggc aagcgaagat caggggcaag gggcgaaatt
     1561 tcgcgccggg acctggctag cgcgtgcgcg ccagcaggat ggaggtcacg agcgcgagaa
     1621 gcgccgtcgg gatccaggcg agcagcaccg ggttcagctg gaacaccacc gtaccgctct
     1681 cgatcagccg ctgcagcagg aagaagccga ggccgatcag cagcccaagc atcatgcggg
     1741 agccggctcc cgccgagcgc atcgagccga gcacgaacgg tatcgctacc agcacggcga
     1801 acatcacccc gacggtgcgc gcgatgcgtg accagaaagc aaacacatat tcacgcgcgt
     1861 cgagcgagtt gctttgaaag tactgaatga ggcgccagag cgcgcgcccg gtgagctgct
     1921 tcgggtcctc gacggcgagc cccagaaacc ccgcggagac gttcgattcg agctcgcgct
     1981 gctccggcgc ttgggtcgtg acgctgtcgg catcgaagcg cgattcgcgg tagtcactca
     2041 gcagccacct gccgcgtccc tcggcggctg catgcgcggc gtgcccgagc gcgatcagac
     2101 ggtgcggcgg cgatagttcg aagatctgca tgccgccgaa ctgccgctcg ctcgtctgcc
     2161 ccgccacgtt gagtatgaga tccctatccc ggacccacgc accgccgtcg ccgccgaagc
     2221 tgacattgct gagcttgctg aaggccttgt atgcgcgcgc ggtcgcctgg aggggtgggg
     2281 cgagagactc gcccaacaga acctcgaaga gcaccagcag gagacccgcg aggagcgcga
     2341 tccttgcaag ccgcacgacc gagatgccgg cggcgcggat aaccgtcagc tcgctgccgc
     2401 gtgcgagcga acccaagccc aggagcgagc cgatgagcgc ggtgatcggc atcagctcat
     2461 aggccagctg cggcagattg agcagcgagt accacagcgc gccgagcgcg ctgtaatggc
     2521 ccacgccgat atccttctgc tgattgataa atacgaacag cgccccgagc acgagcagca
     2581 ccacgatcac catcagcacc gtgccgagga tggtgcgcac gatataacga tcgagtacgc
     2641 tcacaagccg cgcatccgct cgcgcaaacg ggtgatgagg ccgggacccc cctgccgcgg
     2701 tcgcagcttc gagagcggaa ccgcatggag tgccagcagc aggcatgtga ccggcatgct
     2761 gatgtgccag tgaagctcgg cccgctcggc gcggtcgcgt gagtgaggca ggcgcgccga
     2821 tgggcgcacc tcgatgcccc gccccaacac ctcgttcgcg agcagcatga tgagcagcac
     2881 gccgctcacc accaggccgg cgagcaccac ctcacgcagg acaaagcggc ccaggatact
     2941 tccaaacctc atgactaaaa cgcgccgaaa cccgcggcgg gctcccgagt tactctcgcc
     3001 accacttgca cccgtaacgg cgtaggacgc gcttaataaa acgaaccgag cgcgagacgc
     3061 tgtaatgatc gcgaataagg taaatcagac ggcggcgcgc ctcaacggca gaacttctct
     3121 agacgttgct gctcatttcc gaaccgcgcg cggcatagcc atccgcccgc ggttttaccg
     3181 cgcaggccaa gcacgaggca tccgaggtaa ggggacagaa agccaatgat tcgttggtgg
     3241 gtctgagggg atcgaaccgc cgacattcgc cttgtaaggc caggcgatca aagcaactcg
     3301 acgggttagc tcatccaaat cggccgtaaa cgggcgtcaa agaagcacgc cggagctgtg
     3361 ctcggtccac aagcctcaat gcaggagccc gaacacacca acgccagtct tgctgccgac
     3421 aaagacctta ccgtcgacga cggttggaac cgtaaacttg actccagcgc cgaattggtc
     3481 ccgtttgttg gcggcctggg tactgttgta aagctcgact gcaagattcg tggcatcgaa
     3541 tgcgtgcaga atcgccgaac tggcgtggtc tatggcccaa acgataccat tactgttgcc
     3601 gttcgcagag atgactggcg gacctcccgg atatcccaag cacacggaga cagaagtcgg
     3661 cgaggtcgac agtcttgcat cggtgatgga gaacgcctta agaggcgcaa catcggccaa
     3721 gtacagcctg ccgttgaagt aagcgcccgt cgagcgggcc aattgtgata gtgcgccgtc
     3781 cacttcctgc cagatgttat ttcgggagga gttgaatttg ctcatcgaat cccggtcgac
     3841 tatatagatg ttaccgtcct tgccggcacc agcggcgaga tgcttcacgt tgccagatgc
     3901 gtcggtgagg tcaggcagga tgatcggccc gctcgcaccc aaatcaagat cggcaacgga
     3961 ttcactcacg gcgttccaca tcgcgaagta gtccacaacg gcgagctgct gtccggcggt
     4021 tgaaattttg aggtaggagt taccgtaatc ctgagcgctc ggaaagccat tcgcatcgag
     4081 tgtggtttcg aacgcaccgt tacctgccat cacacaaatg tatcctgctg catctgctgc
     4141 gggaccaccg cgccttgcca tatcccaggc cc
//
LOCUS       DATDMP010000473         3458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_743712, whole genome shotgun sequence.
ACCESSION   DATDMP010000473 DATDMP010000000
VERSION     DATDMP010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743712"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VI195_08275"
     CDS             complement(<1..245)
                     /locus_tag="VI195_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824422.1"
                     /translation="MSAAQRVLLINPKMTGKRNARFPLAVLSLRTALEGRHQATILDG
                     NVLQDSVATAARLAGAGVDAVGVSVMGGPQLTSAIAIS"
     gene            complement(242..1711)
                     /locus_tag="VI195_08280"
     CDS             complement(242..1711)
                     /locus_tag="VI195_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6824423.1"
                     /translation="MSEPGGSCVTATPASTRGQRAKVVLYNPQAVFFTMPLALLAIGS
                     ELDPELYEVVIVDGRLEPEPERAVLAHLDGALCLGVTVLTGAPISDALRISRAAKRAH
                     PSLPVVWGGWHPSMFARDCLAEPSVDVTVRAQGEETFAEIVRRLAAGQSLAGCAGCTV
                     RLADGSICENPSRPLAPVDRFRAHDYGLIPVERYFALKGKRQLDYISSQGCAFRCAFC
                     SDPFVYGRKWVGLEPTRMALRLKELWDRYHFDDVNFQDETFFTRARRVQTLADHIIDS
                     GMKITWAGTMRADQGVRLPEEVWARCKQSGLRRLLVGVESGSNEMLRRIRKDIRIEQV
                     FTTAALMLKYGIAGHFPFIVGFPEESDSSIQATLDCAKRLRSLSPDFLTPIFYFKPYP
                     GSELVMEAVARGFRLPDTLEEWSTFDYVAGLPGPWVSAAKYRLIERFKFFQELAWKRV
                     SPATRALQQLARYRCRRDEYRWPVEMLLSRWVLPAERLS"
     gene            complement(1708..2460)
                     /locus_tag="VI195_08285"
     CDS             complement(1708..2460)
                     /locus_tag="VI195_08285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HEY6824424.1"
                     /translation="MLDLTQQPRARLPQEALRPARFPKLARLLRKTLALSDRLAGKMR
                     YDDFRIERVHGVPLLVMPTVFNPLLPRTGAFLAQQLDARRVPADAEVLDMGTGSGVCA
                     LFAARHARRVVAVDINPAAIRCASINALLNHLEQRIEFRHGDLFAPVAGERFDLIVFN
                     PPFLRGVPRSDRDRAWRSSDIAERFAAGLGNHLKPRGAALLVLSTFGDAELFLEQLRQ
                     HGFSISVLAERSFINERLAVFKLERPSPTGAQ"
     gene            complement(2453..>3458)
                     /locus_tag="VI195_08290"
     CDS             complement(2453..>3458)
                     /locus_tag="VI195_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6824425.1"
                     /translation="LERLPLVTLYLSERCNSRCITCDYWRHGVTDMSLDAVRRLLPDL
                     QRLRTQVALISGGEPLLNPEWPQIAELLRREDLKLWLLTSGLSLAKHARRVAALFEAI
                     TVSLDGTDPAPYQAIRGLDAFDTVCAGMRAAVQAGSAVSMRVTLQRANYRQLPQFIAI
                     ARVLGVQQVSFLAVDVANPHAFARRGDFTSGLALQPADLPVFAAILDRLESEHAADFQ
                     SGLIADSARKLRRIHQYFAAICGLAPYPEVRCNVFDYSAVVGASGGVSPCFFISGPDT
                     GALPDGLEATLNEDSMIALRERIRAGARAECARCVCSLWRDPNERGVAHFLPRGRADA
                     "
BASE COUNT          666 a         1148 c         1132 g          512 t
ORIGIN      
        1 gagatggcga tggccgaggt gagctgcggg ccacccataa ccgacacccc caccgcatcc
       61 acacccgcgc cggccaaacg tgcggcagtg gcgacggaat cctgcagcac gttgccatcg
      121 agaatggtcg cctgatgtcg accctccaga gcagtgcgca gactcagcac cgcgagcgga
      181 aagcgcgcgt tgcgcttgcc ggtcatcttg ggattgatga gcagcacgcg ctgcgcggca
      241 ctcacgacag gcgctccgcc ggcagcaccc agcgcgatag caacatttcc accggccagc
      301 gatattcatc gcggcggcag cgatagcgcg ccagttgctg cagggcgcgc gttgcgggcg
      361 aaactcgctt ccaggcgagc tcttggaaaa acttgaaccg ctcgatgagc cgatacttcg
      421 cggccgagac ccacgggccc ggcagacccg ccacgtagtc gaacgtcgac cactcctcga
      481 gcgtgtccgg cagccggaag ccgcgcgcca ccgcctccat gacgagctcg cttccgggat
      541 agggcttgaa gtagaagatc ggcgtcagga aatccggact gagcgagcgc aggcgcttgg
      601 cacagtcgag cgtagcctga atactggagt cgctctcctc tgggaagccg acaatgaagg
      661 ggaaatgccc ggcaataccg tatttcagca tcagcgcggc ggtggtgaac acctgctcga
      721 tgcggatgtc cttgcgaatg cgccgcagca tctcgttcga gccggactcg acgccgacca
      781 gtagtcggcg caatcccgat tgcttgcagc gcgcccacac ctcctccggc agccgcactc
      841 cctggtcggc gcgcatggtg cctgcccagg ttatcttcat gcccgaatcg atgatgtggt
      901 cggccaaggt ctgcacgcgg cgcgcccgcg tgaagaacgt ctcgtcctgg aagttgacgt
      961 catcgaaatg atatcgatcc cagagttcct tcagacgcag cgccatgcgc gtcggctcga
     1021 gccccaccca cttgcgaccg taaacaaacg gatcggagca gaaggcacag cggaaggcgc
     1081 agccttgcga agagatgtag tcgagctgcc gtttgccctt cagcgcgaag tagcgctcca
     1141 ctgggatcag tccgtagtcg tgcgcgcgga accggtcgac cggcgccagc gggcgggagg
     1201 gattctcgca aatgctgccg tccgcgagcc gcaccgtgca tccggcacag cctgccagtg
     1261 actgcccggc ggccagccgc cttacgatct cggcaaaggt ctcctcgccc tgagcgcgca
     1321 cggtgacgtc gaccgatggc tcggccagac agtcgcgcgc gaacatcgac ggatgccagc
     1381 ctccccacac caccgggaga ctcgggtgcg cgcgcttggc agcgcgcgag atgcgcagtg
     1441 catcggagat cggtgcaccg gtcagtaccg tgaccccaag gcatagcgcc ccatcgagat
     1501 gtgcgagcac cgcgcgctcg ggctccggct cgaggcgtcc atcaacgatc accacctcgt
     1561 agagctcagg atcgagctcg gagccgatcg caagtagcgc cagcggcatt gtgaagaaca
     1621 cggcctgcgg gttgtacagc accaccttgg cgcgctgtcc gcgggtcgat gccggcgtcg
     1681 cggtaacgca gctgccgcca ggctcgctca ttgtgccccc gtcgggctcg gccgctcgag
     1741 cttgaagact gccaggcgct cattgatgaa gcttcgctcg gcaagcaccg aaatcgaaaa
     1801 gccatgctgc cgcaactgct cgagaaacag ctcggcatcg ccgaaggtcg acagcacgag
     1861 cagcgctgcg ccgcgcggct tgaggtgatt gcccagccct gcggcaaagc gttcggcgat
     1921 gtcgctcgag cgccacgcgc ggtcgcgatc gctacgcggc acaccacgca gaaagggcgg
     1981 attgaaaacg atcagatcga accgctcacc ggccacgggt gcgaacaggt cgccgtggcg
     2041 aaactcgatg cgctgttcga gatgattcaa cagagcgttg atgctggcgc agcggatcgc
     2101 cgccgggttg atgtccacgg caacgacacg gcgtgcgtgc cgggcggcaa acagcgcgca
     2161 cacgcccgat ccggtcccca tatcgagcac ttccgcgtcg gcgggcaccc ggcgcgcatc
     2221 gagctgctgc gcgaggaatg ctccggtccg cggcagcagc ggattgaaca cagtcggcat
     2281 gacgagcagc ggcacgccgt gcacgcgctc gatgcggaaa tcgtcgtagc gcatcttgcc
     2341 cgcgagcctg tcagacagcg cgagtgtctt gcgaagcagc cgggcgagct tggggaagcg
     2401 cgccggccgc agcgcctcct gcggcaatcg cgcgcgcggc tgctgcgtca gatcaagcat
     2461 cggcgcgccc gcgtggaaga aagtgcgcca cgccccgctc gttcggatcg cgccacagcg
     2521 agcacacgca ccgcgcgcac tccgctcgag cgccagcacg gatgcgctca cgcagcgcga
     2581 tcatcgaatc ctcgttcagc gtcgcctcga gaccatcagg cagggcgcct gtgtcgggcc
     2641 cggaaatgaa gaagcacgga ctcacgccac cgctggcgcc gactaccgcc gagtagtcga
     2701 atacgttgca gcgcacttcc ggatagggtg ccaggccgca gatggccgca aagtactgat
     2761 gaatacggcg tagcttgcgc gcgctgtccg cgatcaggcc cgattggaag tcggcggcgt
     2821 gctcgctctc cagccgatcg aggattgcgg cgaacaccgg caggtcagct ggctgcagag
     2881 cgagaccgct ggtgaaatcg ccgcggcgcg cgaaggcgtg cgggttggcc acatcgacgg
     2941 ccagaaatga cacctgctgc actccgagca cacgcgcgat agcgatgaat tgcggcaact
     3001 gccgatagtt ggcgcgctgt aaggtcacgc gcatgctgac ggcactgcct gcctgcacgg
     3061 cggcacgcat acccgcgcac accgtgtcga aggcatcgag gccgcggatc gcctggtaag
     3121 gtgccgggtc agtgccgtcg agcgatacgg tgatcgcttc gaacagcgcg gccacgcggc
     3181 gcgcgtgctt ggcgagggaa agtccggaag tcaacagcca taacttcaga tcctcccggc
     3241 gcaacagctc ggcgatctgc ggccactcag gattgaggag cggctcaccg ccggagatca
     3301 gcgccacctg agtgcgcaag cgctgcagat ccggcagcag gcggcgcacc gcatcgaggc
     3361 tcatgtcggt aacgccatgg cgccagtagt cgcaggtgat gcaacgcgaa ttgcagcgct
     3421 cgctcaggta gagcgtcacc agcggcaggc gctcgagc
//
LOCUS       DATDMP010000474         3092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1186801, whole genome shotgun sequence.
ACCESSION   DATDMP010000474 DATDMP010000000
VERSION     DATDMP010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186801"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VI195_08295"
     CDS             complement(<1..446)
                     /locus_tag="VI195_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6824426.1"
                     /translation="MAFHIDHLFSLTRDREGARALLTRMGFDLTERGEHPGRGTSNHL
                     AFFGRCYWELLAVDTPTAENAALCERGAGLIGCALRTSDAERDAQQARTLGYEAGEPE
                     SFTRPVRVDGEWQTARFRTVVLRPPIAVDTYLFFCQHLTPQFVWPR"
     gene            complement(494..1576)
                     /locus_tag="VI195_08300"
     CDS             complement(494..1576)
                     /locus_tag="VI195_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6824427.1"
                     /translation="MLSSATAESVLRPNAADLVVSDRPIAGLSWSAYRSVLDALIGRG
                     ASEPAHQSGKPARDARAPGHRVVREYLGPEDGEGFWDYFQLSDELSVSITDATYRQDH
                     WISVEGGRFFKVRVLLSGRLLRPNGELMLQGPQAQLHVCAGERGGGYAIAHGAATRLV
                     VLHCAPELLTRRLGFAAGEAPYPLQALTGREGAGGLAVKIPLTPELLRAAQWIDASRF
                     TVLPAVRSVYLEALAMQLLCHTVTELSVHEPIAGIPPRDVKRIYEARDYLAQHYACPP
                     TIPQLARMVGMNQTKLKAGFKQIFQLTIYAYILQRRMEATAQMLIEREFSVAEIAYRV
                     GYEYPANFSCAFKRYYGCSPRQWKRS"
     gene            1760..2770
                     /locus_tag="VI195_08305"
     CDS             1760..2770
                     /locus_tag="VI195_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY6824428.1"
                     /translation="MRAVLTVGHGARDQLQLAADFPAPKAAPGEVVLKVAATAVNYHD
                     IFTRRGMPGIRIPLPVIVGSDIAGEITELGAGVTGWRIGARVLVDPVFRDGERHGMIG
                     ETAHGGRAEYVAVPAAQLVAVPDAVSLEDAASLPLAYGTAYRMLVTQGRLARGEKILV
                     LGASGGVGVACVQIAKLLGAEVIAGTSSAAKQKPLAALGADHTVNYSAESIVDAVKRL
                     YGKPRVTGGGGVDVAVNYTGGDTWRDTQRTVTSGGRILTCGATAGFEVITDARFLWTF
                     EHHYIGSNGWAVSDLEALLGLIRDGRLKPVIDRVLPIDQAAEGERLLEDREVVGKVLL
                     KP"
     gene            2767..>3092
                     /locus_tag="VI195_08310"
     CDS             2767..>3092
                     /locus_tag="VI195_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6824429.1"
                     /translation="MSEPVSAIAGGNLGKLLRPDVPAGQGALIDLLPPRRAWTCGELD
                     SAADAFADALTRAGVAADARVAILAENCAEHLIAYMGTMRAGAVAVPVNHRLPADTVR
                     FILEDCA"
BASE COUNT          524 a          990 c         1082 g          496 t
ORIGIN      
        1 tcgcgcggcc atacgaactg cggggtcagg tgctggcaga agaagagata ggtatcgacc
       61 gcgatgggcg gtcgcagcac gacggtgcga aaacgggcgg tctgccactc gccatcgact
      121 ctcaccggcc gggtgaagct ctcgggctca ccggcttcgt acccgagcgt gcgcgcctgc
      181 tgcgcgtcgc gctccgcatc gctcgtgcgc aacgcgcacc ctatgagtcc agcgccgcgc
      241 tcacacaacg cggcgttctc ggcggtgggt gtatcgaccg cgagcagctc ccagtagcag
      301 cgcccgaaga aggcgagatg attggaggtg ccacgcccgg gatgctcgcc gcgctcggtc
      361 agatcgaagc ccatacgcgt gagcagtgcc cgggcaccct cacggtcgcg ggtgaggctg
      421 aagagatgat cgatgtgaaa cgccatgaga cgaccgggca ctcagcgccg gaggcgcgca
      481 tgcgccccag ggctcagctg cgcttccact gacgtggcga gcacccgtag taacgcttga
      541 acgcgcagct gaaattggcc gggtactcgt agcccacgcg gtaggcaatc tccgcgacgc
      601 tgaattcgcg ctcgatgagc atctgcgcgg tcgcttccat gcgtcgctgc aaaatgtagg
      661 cgtagatggt gagctggaag atctgcttga agccggcttt gagcttcgtc tggttcatgc
      721 ccaccatgcg cgcgagctgc gggatcgtag gcggacaggc gtagtgctgc gcgaggtaat
      781 cgcgcgcttc ataaatgcgc ttcacatcgc gcggcgggat gccggcgatc ggctcgtgca
      841 ccgagagctc ggtcacggtg tggcacagca actgcatcgc gagtgcttcg agatacacgc
      901 tgcgaactgc cggaagcacg gtaaagcgcg aggcatcgat ccactgcgcg gcacgcagga
      961 gctccggcgt aagagggatc ttcacggcga gaccaccggc tccttcgcga ccggtcagcg
     1021 cctggagcgg atatggtgcc tcgccagcgg cgaacccgag cctgcgggtg aggagctccg
     1081 gggcacaatg cagcaccacg agccgcgttg ccgcaccgtg cgcaatggcg taaccgccgc
     1141 cacgctcgcc ggcacagaca tgcagctgtg cctgcggccc ctgcagcatc agctcgccat
     1201 tgggtcgcag cagccggccg gaaaggagca cccgcacctt gaagaagcga ccgccctcga
     1261 ccgagatcca gtggtcctgc cgataagtcg catcggtgat cgagaccgac agctcatcgc
     1321 tcagttggaa gtaatcccag aagccctcgc cgtcctcggg gccgaggtac tcgcgcacga
     1381 cacggtgccc gggcgcgcgg gcgtcgcgcg cgggcttccc ggactggtgt gccggctcgc
     1441 tcgccccgcg cccgatcagc gcgtcaagca ccgagcggta cgcgctccag gagagcccag
     1501 caatcggccg atccgagacc accaggtcgg cggcgttggg ccggaggacg ctttcggccg
     1561 tagcagagct cagcatcgtt ctcggggctc aggtagcgcg ttgcgatcga ctctatggta
     1621 agcccgtgct gaagccaagc ctcatgccgt ttgggtatat gaggcttccg ttttcgttaa
     1681 agtgtcgtct ggcaccaaat ttcccggatg gaaagttggg ctaccctccg agggacatga
     1741 gcgggcgccg gatatcccca tgagagcggt cctcaccgtc ggacacggtg cgcgtgacca
     1801 gctgcagctg gcagccgatt ttcccgcccc caaggccgcg ccgggagagg tggtgctgaa
     1861 agtagcggcc actgcggtca actaccacga catcttcacg cgccggggta tgcccggcat
     1921 tcgcattccg ttaccggtga tcgtcggctc cgatatcgcg ggtgagatca ccgagctcgg
     1981 tgcgggcgtg acgggctggc gcatcggggc tcgggtgctg gtggaccccg tgttccgtga
     2041 cggtgagcgc cacgggatga tcggcgagac cgcgcacggc gggcgcgcgg agtacgtcgc
     2101 ggttcctgcc gcgcagctcg ttgcagttcc ggatgccgtg tcgctcgagg acgcggccag
     2161 cctgccgctc gcgtacggaa cggcttaccg catgctggtc acccagggac gcctcgcgcg
     2221 cggcgagaag atcctggttc tcggcgcgag cggtggtgtt ggagtggcct gcgtccagat
     2281 cgcgaagctc cttggtgcgg aagtgatcgc gggcacgagc tccgcggcca agcagaagcc
     2341 gctcgccgca ctcggtgccg accacacggt gaactactcc gcggaaagta tcgtggatgc
     2401 ggtgaagcgc ctctacggca agccacgcgt gacaggcggc ggcggggtag atgtggcggt
     2461 gaactacacc ggcggcgaca cctggcgcga cacgcagcgc accgtgacga gcggcggacg
     2521 cattctcacc tgcggtgcga ccgcgggctt cgaggtgatc accgatgcgc gctttctgtg
     2581 gacgttcgag caccactaca tcggctcgaa cggctgggcg gtgtcagatc tcgaggcgct
     2641 ccttggcctg atccgcgacg gccggctgaa accggtgatc gaccgggtgc tgccgatcga
     2701 ccaggcggca gaaggcgagc ggctgctgga agaccgtgag gtcgtcggca aggtgctatt
     2761 gaagccgtga gcgagcccgt gagcgcaatc gccggcggca atctcggcaa actgctgcgg
     2821 ccggacgttc ccgccgggca gggcgcactc atcgacctgc tgccgccgag gcgtgcctgg
     2881 acgtgcgggg agctggatag cgccgcagac gcctttgcgg atgcgctcac gcgcgcgggc
     2941 gtggcggcgg acgcgcgcgt tgcgattctg gccgagaact gcgccgagca cctcattgcc
     3001 tacatgggca cgatgcgcgc gggtgccgtc gccgtgccgg tcaaccaccg gctgccggcg
     3061 gacacggtgc gcttcattct ggaggactgt gc
//
LOCUS       DATDMP010000475         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1743407, whole genome shotgun sequence.
ACCESSION   DATDMP010000475 DATDMP010000000
VERSION     DATDMP010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743407"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VI195_08315"
     CDS             <1..636
                     /locus_tag="VI195_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6824430.1"
                     /translation="EVTQATREGIPVEKGLRAAEVGDEKYDVGGILLARPFKIVRHGP
                     VRLFVNDMAAMLGFYRDDLGYAVTEEIDYRGHRCVFLRANTEHHSLALYPAALREELG
                     LSAHTTLMSFGLQVGSYRQLRDAVDFLAQQGVPIRHLPPELFPGIDYCAFAIDRDGYA
                     FQLYYYMEQVGWDGKPRPAQLRPKIDNSRWPESVAGVSDSFLGEPYLGPLG"
     gene            complement(619..1269)
                     /locus_tag="VI195_08320"
     CDS             complement(619..1269)
                     /locus_tag="VI195_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEY6824431.1"
                     /translation="MANPVPTLSLLETRVLGTLIEKQHTVPDTYPLTLNALVTGCNQK
                     SSRSPVIEATEAEVQAALDSLRSANLIIESSGGRVARYSHNFERVLQVPSQAAALLTV
                     LMLRGPQTVGELRINSERLHKFADISAVEAFLQELAARPAGALVVELPRVPGARENRW
                     AHLLSGMPKGETASVEERPAQEPLGEIAALKARIERLEGELAHLKALVERQLAERT"
     gene            complement(1335..1760)
                     /locus_tag="VI195_08325"
     CDS             complement(1335..1760)
                     /locus_tag="VI195_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824432.1"
                     /translation="MNTAMPRVLLAARPVAREPFRTALIGHADLIETETLHESLLHLK
                     HEPPPALVCCTVLFDESRMFELLRWVKAELPTVAFICARALAWDLTKFTIEAVEIASK
                     SLGATAFIDVPAWTKKYGDAGARERLRLTILAACAGSKS"
     gene            complement(1778..>2242)
                     /locus_tag="VI195_08330"
     CDS             complement(1778..>2242)
                     /locus_tag="VI195_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY6824433.1"
                     /translation="ELLNAMAGIEMTHVPFRGGASPLTEVLSGRVDLLLEAMTLSIGQ
                     IRSGKLRPLAVTSRERWKALPEVPTVAETVPGYEVDSFIGLGASGGTPAAIIDRLNAE
                     VRKALAEPVMHKRLVELGGEPGASSPQEMHDFIAREIAKWKDVIAARKIERL"
BASE COUNT          376 a          757 c          700 g          409 t
ORIGIN      
        1 gaggtgacgc aggcgacgcg cgaaggtatt ccggtcgaga agggcctgcg tgcagcagaa
       61 gtcggcgatg agaaatacga cgtcggcggc atcctgctcg cgcggccgtt caagatcgtg
      121 cgccatggcc cggtacgcct cttcgtcaac gacatggcgg cgatgctcgg cttctaccgc
      181 gacgacctcg gctacgcggt caccgaggag atcgactatc gcggccatcg ctgcgttttc
      241 ctgcgcgcca ataccgagca ccacagcctt gccctctacc cagccgcgtt gcgcgaggag
      301 ctcggcctga gcgcacatac gactctcatg tcgttcggcc tgcaggtggg gagctaccgc
      361 cagctgcgcg atgcagtcga tttcctcgcg caacagggcg tgccgatccg ccatctgccg
      421 cccgagctct tcccgggcat cgattactgc gccttcgcga tcgatcgcga cggctatgcc
      481 ttccagctct actactacat ggagcaggtc ggctgggacg gcaagccccg tcccgcgcag
      541 cttcggccga agatcgacaa cagccgatgg ccggaatcgg tcgccggggt cagcgactcc
      601 ttcctcggag agccgtactt aggtccgctc ggctagctgc cgttccacca acgctttcaa
      661 atgagcgagc tcgccttcga gcctctcgat cctcgccttg agggctgcga tctctccgag
      721 cggctcttgc gccggtcttt cctcgacgga ggcggtctca cctttcggca tgccgctcag
      781 cagatgcgcc cagcggttct cgcgtgcgcc cggcactcgc ggcaactcca cgaccagtgc
      841 gcccgccggc cgcgcggcga gctcctgaag gaatgcctcg accgccgaaa tgtcggcgaa
      901 tttgtgcaac cgctcgctgt tgatccgcaa ctcgcccacg gtctgcgggc cgcgaagcat
      961 cagcacagtg agaagcgccg cggcttgcga gggcacctgc agcacgcgct cgaagttgtg
     1021 cgagtagcgt gcgacccttc cgccgctcga ctcgatgatc aggttggcgc ttctcaggct
     1081 gtcgagcgca gcctgcacct cggcctccgt cgcctcgatc accggggagc ggctcgactt
     1141 ctggttgcac ccggtcacca gcgcattcag cgtaagcgga taggtgtccg ggacggtgtg
     1201 ctgcttctcg atcagcgtgc cgagcacgcg cgtttcgagc agcgatagcg tgggtacggg
     1261 attggccatg gccgcagtct agtgcggttt gtgcgagcgc cgcgcggcgt agggcggctg
     1321 ttacaacgca ggtatcagct tttactgccg gcgcacgcgg ccagaatggt cagcctcaat
     1381 cgttcgcgcg cgccggcatc gccgtacttc ttggtccacg caggcacgtc gataaacgcg
     1441 gttgcgccga ggcttttact ggcgatttct acagcctcta tcgtgaactt cgtcaggtcc
     1501 caggccagcg cacgagcgca aatgaaagcg acggtgggga gctcggcttt cacccatcta
     1561 agcaactcga acatccgcga ctcatcgaac aggaccgtac aacagacgag cgctggcggt
     1621 ggttcatgct tcagatgcag caggctctca tgcaacgtct cggtttcaat caggtctgcg
     1681 tgcccgatca gggcggtgcg gaacggctct cgcgcaacgg gccgggcggc aagaagaacg
     1741 cgaggcatgg cagtattcat ctcgcccagt ctactaccta caggcgctcg atcttccgcg
     1801 cggcgatcac gtctttccac ttggcgatct cgcgcgcgat gaagtcgtgc atctcctgcg
     1861 ggctggaagc gcccggctcg ccgcccagct cgacgagccg tttatgcata acgggctcgg
     1921 caagcgcctt gcgcacctcg gcgttcagcc gatcgatgat cgccgccggc gtaccgccgc
     1981 tagcgccgag gccgatgaac gaatccactt cgtagcccgg caccgtctcg gccaccgtag
     2041 gcacctcggg aagcgctttc cagcgctctc gcgacgtcac ggcgagcggc cgcagcttcc
     2101 ccgagcggat ctggccgatg gaaagcgtca tcgcttcgag cagcaggtca acgcgcccgc
     2161 tcagcacctc ggtgagcggg ctcgcgccgc cgcggaaagg cacgtgcgtc atctcgatgc
     2221 cggccatggc gttcaagagc tc
//
LOCUS       DATDMP010000476         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2188510, whole genome shotgun sequence.
ACCESSION   DATDMP010000476 DATDMP010000000
VERSION     DATDMP010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188510"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VI195_08335"
     CDS             complement(<1..255)
                     /locus_tag="VI195_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824434.1"
                     /translation="MGRLLRHLPMGAQRGTPIPRRPGKRSSAQKARAPSHIGVGAIEI
                     QALRHQLPGLCGIIRAGFRNESYVQSPTDLPRGTPAEPPPL"
     gene            342..860
                     /locus_tag="VI195_08340"
     CDS             342..860
                     /locus_tag="VI195_08340"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6824435.1"
                     /translation="MCNRPDGDIRQHVEAELYCCPDLDETDIAVKVTDGAVTLTGYAR
                     HLLDKYAAEDAAKRVPGVAAVANDIQVQAAAAGTSDPEIARAALAAIRRSVPRCCARI
                     RPVVRQGIVTLEGTVDAEQQRDVAVRAVRDLKGVVCVVNSIAVASEPAQSEPGGDAEQ
                     IRTDPNGRSCTA"
     gene            908..1348
                     /locus_tag="VI195_08345"
     CDS             908..1348
                     /locus_tag="VI195_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6824436.1"
                     /translation="MSLIRWEPFSAMEDMFTRFPATFGRWPRLAGNGDSDLGWAPTAD
                     ISETGEEFLIRASLPGVKKEDVKVTFEDGLLTVSGERQQKEERKDEKYHKIESFYGSF
                     SRSFALPDAINTDAISAESKDGVLTIHVPKTRAEATRPTTIKVQ"
     gene            1431..>2201
                     /locus_tag="VI195_08350"
     CDS             1431..>2201
                     /locus_tag="VI195_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6824437.1"
                     /translation="MNSVAFDAELLRRYDRPGPRYTSYPAAPQFTAVCGAAQLRDSLA
                     RGNALHGTEPLSLYAHVPFCFSPCFYCGCNRIITRDLARGIPYAARLLREIEQLAPLI
                     APRREVVQLHLGGGTPNFLAIETLERLITGIGGALSLSRDPGRDFSIELDPRTTPRDY
                     PAALARLGFNRVSLGVQDFDTEVQRAVNRMQGVEQTLDLIDACRDSGLRSVNIDLIYG
                     LPRQTVAGFRRTLRTVIAARPDRLAVYGYAHLPGLFKAQ"
BASE COUNT          378 a          741 c          742 g          340 t
ORIGIN      
        1 aagcggcggc ggctctgcag gcgtgccgcg aggcagatcg gtcggcgact ggacataaga
       61 ctcattccgg aaacccgccc ggatgatacc gcacaggccc ggcaactggt gcctgagggc
      121 ctgaatttca atcgcgccga cgccaatgtg cgacggcgct cgcgcttttt gggcgctgct
      181 gcgctttccc gggcgacgcg ggatcggcgt tccgcgctgc gcgcccatag gtaggtgccg
      241 cagcaaacgt cccacttctc cgtattggcc cgggcgcacc ggtgtcgata ctggcaggca
      301 gggcgggacg cgcccgcaag ccaggtccgg agtacgcgta aatgtgcaac agaccagacg
      361 gggacatccg gcagcacgtg gaggcggaac tctattgctg ccccgacctc gacgagacgg
      421 atatcgcggt caaggtaacc gacggtgcgg tgactctcac cggctacgcg cgccacctgc
      481 tcgacaagta cgcggccgag gatgccgcca agcgcgtgcc gggcgtcgcg gcggtcgcca
      541 acgacatcca ggtacaggcg gcggccgccg ggacctcgga tccggagatc gcgcgagccg
      601 cgctcgccgc catcaggcgc agcgtgcccc gctgctgcgc gcggatccgc ccggtggtgc
      661 gccagggcat cgtgacgctc gagggcaccg tcgatgcgga gcagcagcgc gacgtggcgg
      721 tgcgagccgt acgcgacctg aagggcgtgg tgtgcgtcgt gaactcgatt gcggttgcga
      781 gcgagcccgc gcagagcgag cccggagggg atgcggagca aatacgcact gatccgaatg
      841 gtcggtcgtg caccgcttga ctagcatttc gccccggagt caagggcaca aagtaaggag
      901 ggaagtcatg agcctcatcc gttgggaacc cttcagcgca atggaagaca tgttcacgcg
      961 tttcccggcc acgttcggcc gctggccgcg gttggcgggc aacggcgatt ccgacctcgg
     1021 ctgggcgccg acggccgata tcagcgagac cggcgaggag tttctcatcc gcgcctcgct
     1081 gcccggcgtc aagaaggagg acgtcaaggt cactttcgag gacggcttgc tgacagtgag
     1141 cggcgagcgt cagcagaagg aagagcggaa ggacgagaag taccacaaga tcgagagctt
     1201 ctacggcagc ttctcgcgca gcttcgcgct cccggatgcg atcaataccg atgcgatcag
     1261 cgccgaatcc aaggacggag tgctgacgat tcacgtccca aagaccaggg ccgaagcgac
     1321 acggccaacg acaatcaagg tccagtagac gtggagttgg cgcattcgct gcgggtggcg
     1381 cacgctaacg cgccgcccgc agcggttctt taccggcctg cggctgcacc gtgaacagcg
     1441 tcgccttcga tgcagagttg ctgcgccgtt acgatcggcc cgggccgcgc tacacctcct
     1501 atcccgccgc gccgcagttc acggccgtct gcggcgcggc gcagttgcgc gacagcctgg
     1561 cacgcggcaa tgcgctgcac ggcactgagc cgctgtcgct ctacgcgcac gttccgttct
     1621 gcttcagccc gtgtttctac tgcggctgca atcgaatcat tacccgcgac ctcgcccggg
     1681 gcatccccta cgccgcgcgc ctgctgcgcg agatcgagca gctggcgccg ctgatcgccc
     1741 cgcggcgtga ggtggtgcaa ctgcacctcg gcggcgggac gccgaacttt ctcgccatcg
     1801 agacgctcga gcggctgatc accggcatcg gcggcgcgct cagcctcagc cgcgaccccg
     1861 ggcgcgattt ttccatcgag ctcgatccac gcaccactcc gcgcgattac cccgcggcgc
     1921 tcgcgcgtct cggcttcaac cgcgtcagcc tcggcgtgca ggacttcgat accgaagtgc
     1981 agcgcgcggt gaaccggatg cagggcgtag agcagaccct cgatctcatc gacgcctgcc
     2041 gcgacagcgg cctgcggtcg gtcaacatcg atctgatcta cggcctgccg cgccagaccg
     2101 tcgcgggctt caggcgcacg ctgcgcacgg tgatcgccgc gcggcccgat cgcctggcgg
     2161 tgtacggcta cgcgcatctg ccgggactgt tcaaggccca g
//
LOCUS       DATDMP010000477         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_77301, whole genome shotgun sequence.
ACCESSION   DATDMP010000477 DATDMP010000000
VERSION     DATDMP010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77301"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1525
                     /gene="carA"
                     /locus_tag="VI195_08355"
     CDS             341..1525
                     /gene="carA"
                     /locus_tag="VI195_08355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY6824438.1"
                     /translation="MSLPAVLALADGTMFRGQSIGARGNTTGEVVFNTALSGYQEILT
                     DPSYARQIVTLTCPHIGNTGTTPEDLESAQIYPAGLVVRDLPVLPSSWRLSESLPEFL
                     ERGRLVAIAGIDTRKLTRILREKGAQAGCIMTGEQLDEAAAIKAARKFPGLKGMDLAK
                     VVSTKRIFQWNEGSIWPAAERPPIRSQQRLHVVAYDFGIKRNILRVLADFGCRMTVVP
                     AQTSAADALALEPDGVFLSNGPGDPEACEYAIAAVRAFLETDLPVFGICLGHQILGLA
                     AGARTVKMKFGHHGANHPVQDLDSGRVFITSQNHGFAVDESTLPAHVKPTHRSLFDGS
                     LQGLAFSERPVFGFQGHPEASPGPHDIKPLFERFNHLMLRRLQAQLKLAEDTLRQHVA
                     GR"
     gene            1525..>2579
                     /gene="carB"
                     /locus_tag="VI195_08360"
     CDS             1525..>2579
                     /gene="carB"
                     /locus_tag="VI195_08360"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY6824439.1"
                     /translation="MPKRTDIESILIIGAGPIVIGQACEFDYSGAQACKALREEGYHV
                     ILVNSNPATIMTDPEMADAIYIEPVNARTVARIIEKERPDALLPTMGGQTGLNTALDL
                     VREGVLERFEVELIAASRAAIDMAEDRELFRNAMREIGLETPQSQIARSLDEALAIAG
                     EIGYPLVVRPSFTLGGSGGGIGYNREELEEIVARGLDASPTGEVLLEESVIGWKEFEM
                     EVVRDHRDNCIVVCSIENLDPMGVHTGDSITVAPALTLTDKEYQRMRDAAVAVLRKIG
                     VDTGGSNVQFAVSPQDGRLLVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNDITGGATPASFEPS"
BASE COUNT          432 a          891 c          855 g          401 t
ORIGIN      
        1 ggggcccggg gaggagctgc gtctcgagca tcgggcgacg gatcgggcgg tgttcgcccg
       61 gggggccctc acggccgcgc tctggctggc gtcacggccg ccggggcgtt acgggatgcg
      121 cgatctgctc gctttaaaaa cagccactta gaacgctgac gtgcgagccg ccgcgaggct
      181 tgcggggccc gcccgggggg gaattttccg tggacacatc gcccgcctag ctcgtaaaat
      241 ttccgcctaa tccgtgtacg ggttagtcca tgcaaggcgg gaggatgttc gatgagaact
      301 cctcccgctt tgtgtatgtg cgccagggag atctgatcac gtgagcttac ctgcggtgct
      361 cgcgctggcc gacgggacga tgttccgcgg ccagtcgatc ggagcgaggg gcaacaccac
      421 gggggaagtc gttttcaata ccgcgctcag cggctaccag gaaatcctca ccgatccctc
      481 ctacgcgcgc cagatcgtca cgctcacctg cccgcacatc ggcaacaccg gcacgacgcc
      541 cgaggatctg gagtcggcgc agatctatcc ggcggggctg gtggtgcgcg atctgcccgt
      601 gctgccgagc agctggcgcc tcagcgagtc gctgcccgaa tttctcgagc gcggccggct
      661 ggtggccatc gccggcatcg acacccgcaa gctcacgcgc atcctgcgcg agaagggtgc
      721 gcaggccggc tgcatcatga ccggcgagca gctcgacgag gcggccgcaa tcaaggcggc
      781 gcgcaaattt cccggcctca agggcatgga cctggcgaag gtcgtgagca ccaagcgcat
      841 cttccagtgg aacgagggca gcatctggcc cgcagccgag cgcccgccga tccgctcgca
      901 gcagcgcctg cacgtggtcg cctacgactt cggcatcaag cgcaacatcc tgcgcgtact
      961 ggccgatttc ggctgccgca tgacggtggt gccggcccag acttcggcag ccgatgcgct
     1021 cgccctcgag cccgacggcg tgttcctctc caacgggccg ggagatcctg aggcgtgcga
     1081 gtacgcgatc gcggcggtgc gcgcctttct cgagaccgac ctgccggtgt tcggcatctg
     1141 cctcggccat cagatcctcg gcctcgccgc cggcgcccgt acggtgaaga tgaaattcgg
     1201 ccatcacggc gccaaccacc cggtgcagga cctcgattcg ggccgggtgt tcatcaccag
     1261 ccagaatcac ggcttcgccg tcgatgagtc gacgctcccg gcgcacgtga agccgaccca
     1321 ccgctcgctg ttcgacggct cgctgcaggg cctcgccttc agcgagcggc cggtgttcgg
     1381 cttccagggc cacccggagg cgagccccgg cccccacgac atcaagccgc tgttcgagcg
     1441 cttcaaccac ctgatgctgc gccgcttgca ggcgcagctg aagctggccg aggacaccct
     1501 gcgccagcac gttgccggcc gctgatgccc aagcggaccg acatcgaaag catcctgatc
     1561 atcggcgccg gtccgatcgt catcggccag gcgtgcgaat tcgattactc cggcgcccag
     1621 gcgtgcaagg cgctgcgcga agagggctac cacgtcatcc tggtcaactc caaccccgcg
     1681 accatcatga ccgacccgga gatggccgat gccatctaca tcgagccggt gaacgcccgc
     1741 accgtggcac gcataatcga gaaggaacgt ccggatgcgc tgctgcccac catgggcggc
     1801 cagaccggcc tcaacaccgc gctcgacctg gtgcgcgagg gggtgctgga gagattcgag
     1861 gtcgagctga tcgccgcctc gcgcgctgcc atcgacatgg ccgaggaccg cgagctgttc
     1921 cgcaatgcga tgcgcgagat cggcctcgag acgccacagt cgcagatcgc ccgcagtctc
     1981 gatgaggcgc tggcgatcgc cggcgagatc ggctatccgc tggtggtgcg cccctcgttc
     2041 acgctcggcg gcagcggcgg cggcatcggc tacaaccgcg aggaactcga agagatcgtc
     2101 gcgcgcggcc tcgacgcctc gccgaccggc gaggtgctgc tggaggaatc ggtgatcggc
     2161 tggaaggaat tcgagatgga ggtggtgcgc gaccaccggg acaactgcat cgtcgtgtgc
     2221 tccatcgaga atctcgatcc catgggcgtg cacaccggcg actcgatcac ggtcgccccg
     2281 gcgctcaccc tcaccgacaa ggagtaccag cgcatgcgcg acgccgcggt ggcggtgctg
     2341 cgcaagatcg gcgtcgacac gggcggctcc aacgtgcagt tcgccgtcag cccgcaggac
     2401 gggcggctgc tggtcatcga gatgaacccg cgcgtgtcgc gctcctccgc gctcgcctcc
     2461 aaggcgaccg gcttcccgat cgccaaggtc gccgccaagc tcgccgtcgg ctatacgctc
     2521 gatgagctga agaacgacat caccgggggc gcgaccccgg cgtccttcga gccgtcgat
//
LOCUS       DATDMP010000478         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_77416, whole genome shotgun sequence.
ACCESSION   DATDMP010000478 DATDMP010000000
VERSION     DATDMP010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77416"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1077
                     /gene="prpB"
                     /locus_tag="VI195_08365"
     CDS             187..1077
                     /gene="prpB"
                     /locus_tag="VI195_08365"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY6824440.1"
                     /translation="MPETSLSAGARLRAALEIERPLQIVGASNAYAALLAERAGFRAV
                     YLSGAGVANASFGLPDLGITSLNDVCEDVRRITAACPLPLLVDADTGFGSAFNIARTT
                     RELIRAGAAGMHLEDQVSAKRCGHRPGKALVHAGEMVDRIHAAVDARNDPAFVLMART
                     DAHAVEGQAAALERAADYVAAGADMIFAEALKTLDEYRQFTSALPVPVLANITEFGQT
                     PLFTATELAAAGVRLVLYPLSAFRAAARAQLQVYAAIRSGGTQRGVLDLMQTRAELYD
                     VLGYHEYEKKLDELFGKEKP"
     gene            1074..2219
                     /gene="prpC"
                     /locus_tag="VI195_08370"
     CDS             1074..2219
                     /gene="prpC"
                     /locus_tag="VI195_08370"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY6824441.1"
                     /translation="MSEMPRFTPKKSVALSGVMAGNTALCTVGRSGNDLHYRGYDILE
                     IATVCEFEEIAYLLIHEKLPNAAELGEYRTRLKALRGLPATVRSVLELLPAATHPMDV
                     LRTAVSVLGCALPEKSAHEPAGAREIADRLIASLGSMLTYWYHFSHSGLRIEVETDDD
                     SIGGHFLHLLHRRAPPPSWVRAMHTSLNLYAEHEFNASTFTARVIAGTGADMYSCITG
                     AIGALRGPKHGGANEAAFEIQQRYATPAAAASDIRARLANKEVIIGFGHPVYTLADPR
                     NQVIKEVARQLSNEAGEPDMFEVAARIEAVMRETKNMFANLDWFSAVSYHMMGVPTEM
                     FTPLFVIARTTGWAAHVIEQRLDGKIVRPTANYTGPEPRPFVPLNAR"
BASE COUNT          374 a          810 c          725 g          364 t
ORIGIN      
        1 gtggcagcgc atcgccaccg acctgcgtcc gcggcacctc gagcgcatcg tgacccgaac
       61 catcgagttc gccgagctgc cggcggcatt ccccgcttac ctcgccgcag gagtcaccgg
      121 gcgcacggtg gtgcgcatcg gctgacggcg cgcgtataga atgattgctc gcttgccagg
      181 tccaccatgc ccgaaacctc actcagcgcc ggtgcacgcc tgcgggctgc cctcgagatc
      241 gaacgcccgc tgcagatagt cggcgcgagc aacgcctacg ccgcattgct cgccgagcgc
      301 gccggatttc gtgcggttta tctttccggc gccggggtcg ccaacgcctc cttcggactt
      361 ccggacctcg gcatcacttc cctcaacgat gtctgcgagg acgtgcgccg catcaccgct
      421 gcctgcccgc tgccgctgct ggtcgatgcc gacaccggtt tcggcagcgc cttcaacatc
      481 gcccgcacca cgcgcgagct gatccgcgca ggtgccgccg gcatgcatct ggaggaccag
      541 gtctccgcca agcgctgcgg gcaccgtccg ggcaaggcgc tggtgcacgc cggcgagatg
      601 gtcgatcgca tccacgccgc cgtcgatgcg cgtaacgacc cggcgttcgt gctcatggcg
      661 cgtaccgatg cgcacgcggt cgaaggccag gccgccgcgc tcgagcgtgc cgcggactac
      721 gtggcggccg gcgccgacat gatcttcgcc gaggccctga agacgctcga tgagtaccgc
      781 cagttcacct ccgcgctgcc cgtgccggtg ctcgccaaca tcaccgaatt cgggcagacg
      841 ccgcttttta cggcgaccga gctggccgcc gccggcgtgc gcctggtgct gtatccgctt
      901 tccgccttcc gcgccgccgc acgggcgcaa ctgcaggtgt acgcggcgat ccgcagcggg
      961 ggcacccaga ggggcgtgct ggatctcatg cagacgcgcg ccgagctcta cgacgtactc
     1021 ggctatcacg agtatgagaa gaagctcgat gagctcttcg ggaaagaaaa gccatgagcg
     1081 agatgcccag gttcacgccg aagaaatcgg tcgccctctc gggcgtgatg gccggcaaca
     1141 ctgcactgtg cacggtgggc cgcagcggca atgacctgca ctaccgcggc tacgacattc
     1201 tcgagatcgc cacggtgtgc gagttcgagg agatcgccta ccttctcatc cacgaaaaac
     1261 taccgaatgc cgccgagctc ggcgagtacc ggacgcggct caaagcgctg cgtggactgc
     1321 cggccacggt gcgcagcgtg ctcgagctgc tccccgccgc aacccacccg atggacgtgc
     1381 tgcgcaccgc ggtttcggtg ctcggctgcg ccttgccgga gaaatccgcg cacgagccgg
     1441 ccggcgcgcg cgagatcgcc gatcgcctca tcgcctcgct cggctcgatg ctcacctact
     1501 ggtaccactt cagtcactcg gggctgcgta tcgaggtcga gaccgatgac gattccatcg
     1561 gtggacattt cctgcacctg ttgcaccggc gtgcgccgcc gccctcctgg gtgcgcgcca
     1621 tgcacacctc tctcaatctg tacgccgagc acgagttcaa cgcctcgacc ttcaccgcgc
     1681 gcgtgatcgc gggcaccggc gccgacatgt actcgtgcat caccggcgcc atcggcgcgc
     1741 tgcgtgggcc gaagcacggc ggcgccaacg aggcggcgtt cgagatccag cagcgctacg
     1801 cgactcccgc ggccgcggcc agcgacatac gtgcgcgcct cgcgaacaag gaggtcatca
     1861 tcggcttcgg acacccggtg tatacgctcg ccgacccgcg caatcaggtg atcaaggaag
     1921 tcgcccggca gctctcgaac gaggccggcg agccggacat gttcgaggtg gccgcgcgca
     1981 tcgaggcggt gatgcgcgaa accaagaaca tgttcgccaa cctcgattgg ttctcggcgg
     2041 tgtcctatca catgatgggt gtgccgacgg agatgttcac gccgctgttc gtcattgcgc
     2101 gtaccaccgg ctgggcggca cacgtcatcg agcagcggct ggacggcaag atcgtgcgcc
     2161 ccacggcgaa ctacaccggc ccggaaccgc gtccgttcgt gccgctcaat gcgcgttgac
     2221 cggcggagca acgcacgtgg ttagcacggc ggcgaccgcg aaccccgacg ctc
//
LOCUS       DATDMP010000479         5563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_300827, whole genome shotgun sequence.
ACCESSION   DATDMP010000479 DATDMP010000000
VERSION     DATDMP010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300827"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1555
                     /locus_tag="VI195_08375"
     CDS             263..1555
                     /locus_tag="VI195_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6824442.1"
                     /translation="MFHPLPIYVGLRYVRARSTRFFVSFITWASLIGVCVGVAALIVI
                     LSVMNGFEGELRDRLLSLSAPVRITAAPAYTHGAVAAAGEATSAPVLPNWPRIVQLAR
                     ATPGVTDAAEYAEVQALAVHGPEMVPVLLRGIDPADPATVAELAHTIAQGHLADITAG
                     SARAIVGEVIAERLGLSPGDSLTLLVPTVGADGTPAPKLRDMHVAGVFEVGLQDHDAT
                     LIFVNLADVRGLGPAGTVSEGLRVRVQDVLAAPQVAARLRAQLPPTLEVSDWTRDNAN
                     YFRAIRIEKTMMSLILLLIVAVAAFNIVAMLVMVVTDKRTDIAIMRTFGAAPRRVMGI
                     FITQGLVIGWLGVALGTALGLLLALHVDTIVPFLEHTFRFHIMDADVYYITAIPSEVH
                     WGNVTAIALAALLLTALATLYPAVRAAGTAPAEALRYE"
     gene            1559..2818
                     /locus_tag="VI195_08380"
     CDS             1559..2818
                     /locus_tag="VI195_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6824443.1"
                     /translation="MSAYEWLIGSRYLRSTHRRGFVSFVALMSVCGLMLGVATLIVVL
                     SVMNGFERELRNRILAVTSHATIAGLNGTLSDWQAVQAAVRRQHGVAAAVPYIESQAM
                     LAHGTALAGANVRGVLPEEERTATGLAQHIVSGGLGELTAGGYGIILGSALAHELHAG
                     VGDSVVLIAPAGTATPTGVVPRMRRFRVVGVFSSGMYEFDRGLALTHLEDAARLFRAA
                     GAVTGLRLAFDDPLRAPTLVRQVARRVSQQLGIQGFYVTDWTQDHANFFRSIEITKSM
                     LFVILLMLVAVAAFNIVATLVMIVKEKQTDIAILRTLGAAPRNVLLTFAVQGVLIGLA
                     GTIAGAALGTLLADNLETLVAGLERLLGTQFLDARVYYMSDLPAYVELGDVLRVCAVA
                     LALCVLATIYPAWRAARTAPAQALRHE"
     gene            2811..3494
                     /gene="lolD"
                     /locus_tag="VI195_08385"
     CDS             2811..3494
                     /gene="lolD"
                     /locus_tag="VI195_08385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEY6824444.1"
                     /translation="MSDTVLEAEAVHKSFRQGPVVLEVLRGVALKVAAAERVAIVGAS
                     GSGKTTLLQILGGLDPPSSGVVRVAGRDIHSLSESERGVLRNRTLGFVYQFHHLLPEF
                     SALENVAMPLLVRRSKVAAAREAARALLARVGLAERLDHRPHQLSGGERQRAAVARAL
                     VTQPRVVLADEPTGNLDGANAESVFALMLELNRERGTSLVVVTHDVRLAGRMERIYEL
                     EGGALAVRQ"
     gene            complement(3523..4047)
                     /locus_tag="VI195_08390"
     CDS             complement(3523..4047)
                     /locus_tag="VI195_08390"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY6824445.1"
                     /translation="MPRQLLKRIVPPPQSLRDRWFVRLFGERIADPHLWTLHRRGVTY
                     AFGAGLAICFIPLPVHLLTACTIAIIWRLNIPVICGTTFLVNPFTFMPVYYFAYRMGS
                     ALLHSPPGHFKFHPTWEWFRHGLAPVWQPFVIGCIACAVLFGVLGWLCLELLWRWHVR
                     SRYRSRHATQSASA"
     gene            4182..4856
                     /locus_tag="VI195_08395"
     CDS             4182..4856
                     /locus_tag="VI195_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY6824446.1"
                     /translation="MWEIVRAGGPLMWPIILCSITAAAIVLERLWTLQDKRVLPRELP
                     QKVWGLIEANQVNDKVIAALEQNSPLGRLLATGLANRHRPREMLMERLEDTGRHVVHE
                     LERFLNTLGTIAGVSPLLGLLGTVTGIIRSFNAIEAGGMGDPRALSGGIAEALICTVA
                     GLCVAIPALIFYRYLRGRVDGIVVEMEKQAIRMADALEAAQTRGRERPAPAAVPFEPV
                     RSEAVR"
     gene            4853..5284
                     /locus_tag="VI195_08400"
     CDS             4853..5284
                     /locus_tag="VI195_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY6824447.1"
                     /translation="MNLKTRRHEEPEINVVSLIDVVLLLVVFFILSSKFTDEGRLRVH
                     LPQASAVPTQKVSGEPLVVSVTQQGSYLVNQRELINSSPETLRTALLKEAGGNRNVRV
                     TLRADARATHQAVITAMDVLGRLGFAEVNIATVKEEPAGKP"
     gene            5281..>5563
                     /locus_tag="VI195_08405"
     CDS             5281..>5563
                     /locus_tag="VI195_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824448.1"
                     /translation="MNDSSRAPSVTGSKPPPLDRGALPTYRRLLGYLAAHRGRFVVGM
                     LGGTLFAATMTSFAWFAKKFGDGTLVHEDPRTIVWVPLALVGLFALRSIG"
BASE COUNT          847 a         1868 c         1935 g          913 t
ORIGIN      
        1 tggtgccgcg ctttggccga gcgagctgcg agtcacgcac ctgcacctga tcgttcacca
       61 cgagggtttc ggcaacggcg gcgctggtga gcgcacagcc gcacagcagt gcggcgagaa
      121 cgcggttctg catgcgacaa ctctcctcga gactcgtccc gactataaca ccgcacgaaa
      181 tctgtcaaag cccgctgcgc cggcagctcc gcgatttgtg acgcaagtct gataacggcc
      241 gaactgtggt taaatcgcga cgatgtttca tccactgccg atctatgtcg gcctgcgcta
      301 cgtgcgcgcg cgctcgaccc gtttcttcgt gtcgttcatc acctgggcct cgctcatcgg
      361 ggtgtgcgtg ggagtggccg cactcatcgt catcctgtcg gtaatgaacg gcttcgaggg
      421 cgagctgcgc gaccggctgc tctcgctcag cgccccggtg cgcatcacgg cggcgcccgc
      481 gtacacgcac ggcgctgtgg cggctgccgg cgaggccaca agcgcgccgg tgctaccaaa
      541 ctggccgcgc atcgtgcagc tcgcccgcgc caccccggga gtcaccgatg ccgccgaata
      601 cgccgaggtg caggcactgg cggtccacgg ccccgagatg gttccggtgc tgttgcgcgg
      661 catcgacccc gccgacccgg cgaccgttgc cgagctcgcg cacaccatcg cccaggggca
      721 ccttgcggat atcaccgccg gctccgcacg ggcaatcgtg ggcgaggtga tcgccgagcg
      781 gctcgggctg tccccgggag atagcctgac gctgctggtg cctaccgtcg gtgccgacgg
      841 aacgccagcg ccgaagctgc gcgacatgca cgtcgccggc gtgttcgaag tcgggctgca
      901 ggaccacgac gccacgctga tattcgtcaa tcttgccgat gtgcgcggcc tcggaccggc
      961 cggtacggtg agcgaggggc tgcgggtgcg ggtgcaggat gtgctcgccg cgccgcaggt
     1021 tgccgcgcgg ctgcgggcgc agctgccgcc gacactcgag gtgagcgact ggacgcgcga
     1081 caacgccaac tattttcgcg ccatccgcat cgagaagacg atgatgtcgc tgatcctgct
     1141 gctgatcgtc gcggtggccg cattcaacat cgtcgcgatg ctggtgatgg tggtgaccga
     1201 caagcgcacc gacatcgcca tcatgcgcac tttcggagcg gcgccccgcc gggtgatggg
     1261 catcttcatc acccaggggc tggtgatcgg ttggctcggg gtggcactcg ggacggcgct
     1321 cggcctgctg ctcgcgctgc acgtcgatac catcgtgccg tttctcgagc acacctttcg
     1381 cttccatatc atggacgcgg acgtctacta catcacggcg attccctccg aggtgcactg
     1441 gggtaacgtg accgccatcg cgctcgccgc gctgctgctg acggcgctcg ccacgctcta
     1501 cccggcggtg cgcgcggcag gtaccgcgcc cgccgaggcg ctgcgttacg agtagccgat
     1561 gagcgcctac gagtggctga tcggctcgcg ttacctgcgc tcgacgcacc ggcgcggctt
     1621 cgtatccttt gtcgcgctca tgtcggtgtg cgggctgatg ctgggggtgg cgaccctgat
     1681 cgtggtgctg tccgtgatga acggcttcga gcgcgagctg cgcaaccgca ttctcgcggt
     1741 gacctcacat gccaccatcg ccgggctcaa cggcacgctg tccgactggc aggcggtgca
     1801 ggctgccgtg cgccgccagc acggcgttgc ggcagcggtt ccctatatcg agagccaggc
     1861 gatgctcgcc cacggcaccg ccctggccgg cgccaacgta cgcggcgtgc tcccggagga
     1921 ggagcgcacc gccaccggcc tcgcgcagca catcgtgtca ggggggctgg gagagctcac
     1981 cgcaggcggt tacggcatca ttctcggcag tgcacttgcg cacgagctgc acgccggggt
     2041 cggtgacagt gtcgtgctga ttgcacccgc aggcaccgcc accccgaccg gcgtggtgcc
     2101 gcgcatgcgc cgcttccgcg tggtgggcgt gttcagcagc ggcatgtacg agttcgaccg
     2161 cgggctcgcg ctcacgcacc tcgaggatgc ggcgcggctg tttcgcgcag ccggcgccgt
     2221 caccgggctg cgtctggcct tcgacgatcc gctgcgcgcg ccgaccctcg tgcgccaggt
     2281 cgcgcggcgc gtgtcgcagc agctcggcat ccagggcttt tacgtgaccg actggaccca
     2341 ggatcacgcc aatttctttc gctccatcga gatcaccaag tcgatgctgt tcgtgatact
     2401 gctcatgctg gtcgccgtgg cggcgttcaa catcgtcgcc acgctggtga tgatcgtgaa
     2461 ggagaagcag acggacattg ccatcctgcg cacgctcggg gcagccccgc gcaacgtgct
     2521 gctcactttc gccgtgcagg gggtactcat cgggctcgcc ggcaccatcg ccggggcggc
     2581 gctcggtacg ctgcttgccg acaatctgga gacgctggtg gccgggctcg agcggctgct
     2641 cggcacgcag ttcctcgacg cacgtgtcta ctacatgagc gatctgccgg cgtatgtcga
     2701 gctcggcgat gtgctgagag tgtgcgccgt cgcgctcgcg ctgtgcgtgc tcgccacgat
     2761 ctatcctgcc tggcgtgcgg cgcgtaccgc gcccgcgcag gcgctgcgcc atgagtgaca
     2821 ccgtcctcga agccgaagcg gtgcacaaga gcttccgcca ggggccggtg gtgctcgagg
     2881 tgttgcgggg cgtggcactc aaggtggccg cggcagagcg cgtggccatc gtcggcgcct
     2941 cgggctcggg caagaccacg ctgctgcaga ttctcggtgg cctcgatcca ccgagctcag
     3001 gggtggtgcg ggtggcggga cgcgacattc actccctgag cgagtccgag cgtggcgtgc
     3061 tgcgcaaccg cacgctcggc ttcgtgtacc agtttcacca tctgttgccg gagttctccg
     3121 cgctcgagaa cgtcgcgatg ccgctgctgg tccggcgcag caaggtcgcc gcggcacgcg
     3181 aagccgcgcg cgccctgctc gcgcgtgtag gcctcgccga gcggctcgac catcggccgc
     3241 accagctctc cggcggcgag cgccagcggg cggcggtcgc gcgcgcgttg gtgacgcagc
     3301 cgcgcgtcgt gctcgccgat gagcccaccg gcaacctcga cggcgccaac gccgagagcg
     3361 tgtttgcgct gatgctggag ctcaatcgcg agcgtggcac gagcctggtg gtcgtgaccc
     3421 acgacgtgcg gctcgctggc cgcatggagc ggatctacga gctcgagggg ggagccctgg
     3481 ccgtgcgcca gtagtcgcgg gtgcttggcg cgcgcggcgg cgtcaggccg aggcggattg
     3541 cgtggcgtga cgcgagcgat agcggctgcg cacgtgccaa cgccacagca gctcgaggca
     3601 cagccatccc agcacgccga acagcacggc gcaggcgatg caaccgatga cgaagggctg
     3661 ccacaccggg gcgagtccgt gccggaacca ctcccaggtg ggatggaact tgaagtggcc
     3721 cggcgggcta tgcagcagcg cgctccccat gcggtaggcg aagtagtaga cgggcatgaa
     3781 ggtgaacgga ttcacgagaa aggtcgtgcc gcagatcacc gggatgttga gccgccagat
     3841 gatcgcgatg gtgcaggcgg tgagcaggtg caccggcagc gggatgaaac agatggcgag
     3901 ccccgctccg aaggcgtagg tcacgccacg gcggtgcagg gtccacaaat gggggtcggc
     3961 gatgcgctcg ccgaacagcc ggacgaacca gcgatcccgc agcgactgcg ggggcgggac
     4021 gatccgtttc agcagctgac gcggcatggg cccggatgac tgtaccagaa cgccccggcg
     4081 cgcaatgtgc actgcgcccg ggacgcagtt ccttgagcgc ccccggccct ccggtatgat
     4141 gcgcgctttg cacttgcgac ccgtgtaagg gatttcgtcg gatgtgggaa atagtgcggg
     4201 ccggcggccc gctgatgtgg ccgatcattc tctgctcgat caccgcggcg gccatcgttc
     4261 tggagcggct gtggacgctg caggacaagc gggtgctgcc gcgcgagctg ccgcagaagg
     4321 tgtgggggct catcgaggcc aatcaggtga acgacaaggt gattgcggcg ctcgagcaga
     4381 actcccccct cgggcgcctg ctcgccaccg gcctcgccaa ccgccatcgc ccgcgcgaga
     4441 tgctgatgga gcgcctcgag gacaccggcc ggcacgtggt gcacgagctg gagcgctttc
     4501 tcaacaccct cggcaccatc gccggcgtgt cgccgctgct cggcctgctc ggcaccgtga
     4561 ccggaatcat ccgctccttc aacgccatag aggcgggcgg catgggtgat ccgcgcgcgc
     4621 tctcgggcgg catcgccgag gcgctgatct gtacggtcgc cggtctgtgc gtcgccattc
     4681 cggcgctcat cttctaccgc tacctgcgcg gcagggtcga cggcatcgtg gtggagatgg
     4741 agaagcaggc catccgcatg gccgatgcgc tggaggccgc ccagacgcgc ggccgagaac
     4801 ggccggcccc tgcggcggtg ccgttcgagc cggtgcgctc cgaggccgtc agatgaacct
     4861 caagacccga cgccacgagg agccggaaat caacgtcgtc tcgctgatcg acgtggtgct
     4921 gctgctggtg gtctttttca tcctgtcgag caaattcacc gacgaagggc ggctgcgggt
     4981 acacctgccg caggcgagcg cggttcccac ccagaaggtg agcggcgagc cgctggtggt
     5041 gagcgtgact cagcagggga gttacctggt gaatcagcgc gagctcatca actccagtcc
     5101 cgagaccctg cggacggcgc tgctcaagga ggcgggcggc aaccgcaacg tgcgcgtgac
     5161 gctgcgggcc gatgcccgcg ccacccacca ggccgtgatc accgccatgg atgtgctcgg
     5221 gcggctgggc ttcgctgagg ttaatatcgc gacggtcaag gaagagcccg caggcaagcc
     5281 ttgaacgaca gcagccgagc gccgagtgtc acggggagca agccgccgcc cctcgaccga
     5341 ggtgccctac ccacctatcg ccggctcctc ggctacctcg ccgcgcaccg cggccgcttc
     5401 gtagtcggca tgctcggcgg cacgctgttc gccgcaacca tgacgagctt cgcatggttc
     5461 gccaagaaat tcggcgacgg cacgctcgtg cacgaggacc cgcgcaccat cgtctgggtc
     5521 ccgctcgctc tggtggggct gttcgcgctg cgcagcatcg gtg
//
LOCUS       DATDMP010000480         2898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1300693, whole genome shotgun sequence.
ACCESSION   DATDMP010000480 DATDMP010000000
VERSION     DATDMP010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300693"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1223)
                     /locus_tag="VI195_08410"
     CDS             complement(303..1223)
                     /locus_tag="VI195_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6824449.1"
                     /translation="MTAFLLMLALVVGTGAYVRWALGWIDHGASGVWFVLGAPIAYLT
                     PALVLVTLWFAMAWIWRSPRPPNARIGLKATLRLYWTEVWVIAISWPLMALHRALIRD
                     PAPVPAARPVLLVHGVLVNDGVWLYFRRYLERHGIRPVYTINYGPPLADIERFAEQLA
                     AKTEAICAATGAQRVVLIGHSMGGLVARAYLRRFGARRVERLITLGTPHHGSVLAYLF
                     PGRSLAQLRPGNDWLAELNRDEQRPAPVPITSIWSRHDSMVAPQASAVLGQAENIALL
                     GVGHNALLADSEVRKLVLAAASSVQPISTT"
     gene            complement(1237..2160)
                     /locus_tag="VI195_08415"
     CDS             complement(1237..2160)
                     /locus_tag="VI195_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY6824450.1"
                     /translation="MANEETLFNISAVERETGLSKDVLRIWERRYGFPRPERDENAER
                     QYTAEELSRLRTIKRLMDTGLRPGKLIRQPLEELNALAEKRIRPRRETPPPAVESDVL
                     ALLKGHDVSALHAALTGWLLRHGLQRFVLETAAPLNRAVGEAWMHGDLQVFEEHLYSE
                     LLQLVLRGAINAMPRGHGTPRIVLTTLPGEQHGIGLLMSEALLASEGGQCISLGTQTP
                     FEDIRRAVVAFKAQVLAVSFSTAFPLRQAGDAIVALRRAIASSVVLWAGGAITRRLRR
                     TPAGVRLLPDIADVLPALQAWRTEPGASGAR"
     gene            2367..2885
                     /locus_tag="VI195_08420"
     CDS             2367..2885
                     /locus_tag="VI195_08420"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY6824451.1"
                     /translation="MAAAAACPALLDREFNRLQTGKPESLCQYRGKVLLIVNTASYCG
                     YTHQYEGLEALYRKYKDRGLVVLGFPTNDFGEQEPGSNKEIAEFCRLTYGVQFPMFEK
                     TTVKTLAANPLYTDLVARTGQGPKWNFHKYVVDRNGNPVATFASRVEPNDRELVALIE
                     KLLADRPVEPQG"
BASE COUNT          519 a          984 c          930 g          465 t
ORIGIN      
        1 cggtagcttc tgagccccgg agcgcacgca ctcaacagga ttgccgagcc aagcaccgcc
       61 aataaagcaa tggacttttt catccctctc ccctttctag gtcggttctg tagacaaccg
      121 ataccccgtt tccacgccgc cgactgatcc acataatcga tctcgaactt ttaattcgtt
      181 gagcgtgctg ctgagcacgc tctgaggctg ccgtgtttgt tctccctact ccgtcggtct
      241 ggaacctggg cactgtagcc taccacaacc ggtaaggatc gtttgtcagg aacgcgcagc
      301 gcctacgttg tcgaaatggg ctgaacactg ctcgccgctg caagcaccag cttccttact
      361 tccgaatccg ccaacagcgc gttgtgtccg acgccgagga gcgcgatgtt ttccgcttga
      421 ccgagcacag cgcttgcctg cggcgcgacc atgctgtcgt gccgggacca gatcgaggtt
      481 atcggcacgg gcgccggacg ctgctcgtcg cgattgagct ccgcgagcca atcatttcca
      541 gggcgtagtt gagccaggct ccggcccggg aaaaggtagg cgagcacgct gccatgatgc
      601 ggcgtgccca aagtgatcag tcgttcgacg cgccgggcgc cgaagcgcct gagataggcg
      661 cgggcgacga ggccacccat gctgtgcccg atcagcacca cgcgctgcgc tccggtcgcg
      721 gcgcagatgg cttccgtctt tgcagcgagc tgctcggcga agcgctcgat gtcggcgagc
      781 ggcgggccgt agttgatagt gtagaccggt cggatgccgt ggcgctcgag atagcggcgg
      841 aagtacagcc agacgccgtc gttcaccagg acgccatgga cgaggagcac gggccgcgcc
      901 gccgggacgg gtgcgggatc gcggatcaat gctcgatgca gcgccatgag cggccacgag
      961 atcgcgatca cccagacctc cgtccagtaa aggcgcagcg tcgccttcaa gccgatgcgc
     1021 gcgttcggcg ggcgcgggct gcgccagatc cacgccatcg cgaaccagag cgtgaccagc
     1081 accaatgccg gcgttaagta ggcaatcggc gcgccgagca cgaaccacac gcctgacgca
     1141 ccgtggtcga tccatcccag cgcccagcgt acgtacgcgc ccgtgccgac gaccagcgcc
     1201 agcatgagca gaaacgcggt cacagggcga accgcctcaa cgcgcgcctg aagcacccgg
     1261 ttcggtgcgc cacgcctgca gcgccggcaa cacgtcggcg atgtccggca gcagtcgcac
     1321 gcccgcgggc gtcctgcgca gccggcgggt gatcgcaccg ccggcccaca gaacgacgct
     1381 ggacgcgatg gcccggcgaa gcgcaacgat ggcgtcgccg gcctggcgca gcggaaaggc
     1441 ggtcgagaaa gaaacggcga gcacttgcgc cttgaacgcg accaccgcgc ggcggatgtc
     1501 ctcgaaaggc gtctgcgtgc cgagcgagat gcattggccg ccttcggatg cgagcagcgc
     1561 ctcgctcatc agcagcccta tcccgtgctg ctcgcccggc agggtggtga gcacgatccg
     1621 gggcgtcccg tggccgcgcg gcatcgcgtt tatggcgccg cgcagcacga gctgcagcag
     1681 ctcgctgtaa agatgctcct cgaacacctg caggtcgccg tgcatccatg cctcgccgac
     1741 ggcgcgattg agcggtgcgg ccgtctcgag cacgaaacgc tgcagcccat ggcgcaggag
     1801 ccagccggtg agcgcggcgt gcagcgccga cacgtcgtgt cccttgagca gcgccagcac
     1861 gtcgctctcc acggccggcg gcggggtttc gcggcgcggg cggatgcgct tctcggcgag
     1921 cgcgttcagt tcctcgagcg gctggcggat gagcttgccg ggccgcaagc ccgtgtccat
     1981 cagccgcttg atcgtgcgca gccgcgagag ctcctcggcg gtgtactggc gctccgcatt
     2041 ctcgtcgcgc tccggacgcg ggaagccata gcggcgctcc cagatacgca gcacatcctt
     2101 ggacaggccg gtctcgcgct cgacggcgct gatgttgaac agcgtttcct cgttcgccat
     2161 ggcctcttcg tccggtatgc agcacaagac tagcacgccg ccgacgacgg ctgtcgagcc
     2221 gctgtccaag acaaacggcg atgctatact tttctcttcc gaggagggct ccgatggctc
     2281 cgcaagcaac cgtttttagc ctcgccctgg cgctgtcggc actgacgctg acgccggctg
     2341 cacccgccct cgccgaccga accaacatcg ccgcagcggc ggcgtgcccg gcgctcctgg
     2401 atcgcgagtt caaccggctg cagaccggga agccggagtc gctatgccag taccgcggca
     2461 aggtgctgct aatcgtcaac acggcgagct actgcggcta cacccatcaa tacgaaggcc
     2521 tcgaggccct gtaccgcaag tacaaggacc gcggtctggt cgtgctcggc tttccgacga
     2581 acgatttcgg cgagcaggag ccaggcagca acaaggagat cgccgagttc tgccggctca
     2641 cctacggcgt gcaattcccc atgttcgaaa agacgaccgt gaagactctc gccgccaatc
     2701 cgctctacac cgatctggtg gcccgcaccg gccaggggcc gaagtggaac ttccacaagt
     2761 acgtcgttga ccgcaacggc aatccggtcg cgacgttcgc aagccgggtc gagccgaacg
     2821 accgggagct cgtcgcgctg atcgaaaagc tgctcgccga taggcctgtc gagccgcagg
     2881 gttgaaacgc ccgctgcc
//
LOCUS       DATDMP010000481         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_633726, whole genome shotgun sequence.
ACCESSION   DATDMP010000481 DATDMP010000000
VERSION     DATDMP010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633726"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="secY"
                     /locus_tag="VI195_08425"
     CDS             <1..859
                     /gene="secY"
                     /locus_tag="VI195_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY6824452.1"
                     /translation="GTMFLMWLGEQITERGIGNGISMIILSGIVAGLPGAVGNTMESV
                     SNGEMPGPVALALLFLVAGVTFFVVFMERAQRRIPVDYAKRQVGRRMYAGQSSHLPFK
                     INMSGVIPPIFASSLLLFPATLASFAGSSSQGWFGNAMQDVAAALGYGQPLHLVIYAV
                     LIIFFCFFYTALVFNARDTADNLKKSGAFVRGIRPGQQTAEYIDQVLTRLTLIGAIYV
                     TLVCLVPEILTGWYGVPFRFGGTSLLIVVVVVMDFMAQLNAHMMSHHYPGLLKKANLL
                     GYGRSGPGG"
     gene            863..976
                     /gene="rpmJ"
                     /locus_tag="VI195_08430"
     CDS             863..976
                     /gene="rpmJ"
                     /locus_tag="VI195_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY6824453.1"
                     /translation="MKVRPSVKKICKNCKIVRRRRVVYVICTDPRHKQRQG"
     gene            989..1369
                     /gene="rpsM"
                     /locus_tag="VI195_08435"
     CDS             989..1369
                     /gene="rpsM"
                     /locus_tag="VI195_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY6824454.1"
                     /translation="MARIAGINIPMNKHVWVGLTHIYGVGRARARGACEAAGVNPATK
                     VKDLTEAEINAIRSAIAKFPVEGDLRREVSMNIKRLMDLGSWRGIRHRRGLPVRGQRT
                     RTNARTRKGPRKQAVKLNAPAGKV"
     gene            1496..1858
                     /gene="rpsK"
                     /locus_tag="VI195_08440"
                     /pseudo
     CDS             1496..1858
                     /gene="rpsK"
                     /locus_tag="VI195_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
     gene            1980..2603
                     /gene="rpsD"
                     /locus_tag="VI195_08445"
     CDS             1980..2603
                     /gene="rpsD"
                     /locus_tag="VI195_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY6824455.1"
                     /translation="MARYTGPKCKLARREGTDLFLKSGVKPLENKCKFTVPPGGLKSE
                     RRTRLSDYGLQLREKQKLRRMYGVLERQFSNYYKAAARKTGATGETLLRLLECRLDNV
                     VYRMGFAATRAEARQLVSHKSINVNEKPVTIPSYQVKAGDIVQVREKSRKQLRIQSAL
                     NVAQQVGLPEWVEVNDKEFRGVFKSAPGRDDVLPDINENLVVELYSK"
BASE COUNT          491 a          904 c          950 g          414 t
ORIGIN      
        1 gggaacgatg ttcctgatgt ggctcggcga gcagatcacc gaacgcggca tcggcaacgg
       61 catctcgatg atcattctct ccggtatcgt cgccgggctc ccgggcgcgg taggcaacac
      121 catggagtcg gtcagcaacg gcgagatgcc aggcccggtg gcgctcgcgc tgctgttcct
      181 ggtcgcgggc gtcaccttct tcgtggtgtt catggagcgc gcccagcggc gcattccggt
      241 cgactacgcc aagcgccagg tcggccggcg catgtacgcg gggcagtcga gccacctgcc
      301 gttcaagatc aacatgtcgg gagtgatccc gcccatcttc gcctccagcc tgctgctgtt
      361 cccggcgacg ctggcgagtt tcgcgggctc gagctcccag ggctggttcg gcaacgccat
      421 gcaggacgtg gctgcggcgc tcggctacgg gcagccgctg cacctggtga tctacgcggt
      481 gctgatcatc ttcttctgct tcttctacac cgcgctggtg ttcaacgcgc gcgacacggc
      541 cgacaacctg aagaagtcag gcgccttcgt gcgcggcatc cgccccggac agcagaccgc
      601 cgaatatatc gaccaggtgc tcacgcgcct cacgctgatc ggcgcgatct acgtgacgct
      661 ggtgtgcctg gtgccggaga tcctcaccgg ctggtacggc gtgccgttcc gcttcggcgg
      721 cacctcgctc ctcatcgtgg tggtggtggt gatggacttc atggcgcagc tcaacgcgca
      781 catgatgtcg caccactacc ccgggctcct caagaaggcc aacctgctcg gctacgggcg
      841 cagtgggccg ggtggttgat gcatgaaagt acgtccgtcg gtcaagaaga tctgcaagaa
      901 ctgcaagatc gtgcgccgcc ggcgcgtggt gtacgtcatc tgtacggacc cgcgccacaa
      961 gcagcgtcag ggttgaggtg agagacacat ggcacgcata gccggtatca acatcccgat
     1021 gaacaagcac gtgtgggtcg ggctgacgca catctacggc gtgggacgcg cgcgcgcccg
     1081 cggcgcctgc gaggccgcgg gggtcaatcc cgccaccaag gtgaaggatc tcaccgaggc
     1141 ggagatcaac gcgatccgct cggcgatcgc caaattcccg gtcgagggcg acctgcggcg
     1201 cgaggtgtcg atgaacatca agcggttgat ggatctcggc agctggcgcg gcatccgtca
     1261 tcggcgcgga ctcccggtgc gcggccagcg cacccgtacc aacgcgcgca cccgcaaggg
     1321 cccgcgcaag caggcggtga agctgaacgc gcctgccgga aaggtctgag gcatggctga
     1381 gcagaaaccc cagggtagcg ccgccccggc ggcgccggcg gcgccggcgg cgccggcggc
     1441 tcccgcggca ggcgcggcac ccgcggccgc ggcggcgccc acgccgaaga agcccaagaa
     1501 ggcgcgcaag aacgtgctcg atgcgatcgc gcacgtgcat gcctccttca acaacaccat
     1561 catcaccatc accgaccgcc aggggaacac gctctcgtgg gcgaccgccg gcggttgcgg
     1621 cttccgcggc tcgcgcaaat cgacgccgtt cgccgcgcag gtggcggcgg agaaggccgg
     1681 cgtcgcggct caggagcacg gcgtcaagaa cgtcgaggtg cgcgtcggcg gccccggtcc
     1741 cgggcgcgaa tccgcggtgc gcgcgctcaa caactgcggg ctcaagatca ccagcatctc
     1801 ggacgtgacg ccgattccgc acaacggatg ccgtccgccg aagaagcgga gagtgtaatg
     1861 acgactgcgc gaacaccttg cccgcgggcg aaagccaggt ctttcgcccg cctcgaacga
     1921 agccggcagc gcttgtccgc tgaaagcgcc gcaggcgcct ttcagccaga ggaaagataa
     1981 tggcgcgtta caccggaccg aagtgcaagc tcgcgcgtcg cgagggcacc gacctgttcc
     2041 tgaagagcgg cgtcaaaccg ctcgagaaca agtgcaagtt caccgtgccg cccggcggcc
     2101 tgaagagcga gcgccgcacg cggctctcgg actatggcct gcagctgcgc gagaagcaga
     2161 agctgcggcg catgtacggg gtgctcgagc ggcagttcag caactactac aaggcggcgg
     2221 cccgcaagac cggcgccacc ggcgagacgc tgctgcggct gctcgagtgc cgcctcgaca
     2281 acgtggtgta ccgcatgggc ttcgcggcca cgcgcgccga ggcgcgccag ctggtgagcc
     2341 acaagtcgat caacgtgaac gagaagccgg tcaccatccc ttcctaccag gtgaaggccg
     2401 gggacatcgt gcaggtgcgc gagaagtcgc gcaagcagct gcgcatccag agcgcgctca
     2461 acgtcgcgca gcaggtgggg ctgcccgagt gggtcgaggt gaacgacaag gagttccgcg
     2521 gcgtgttcaa gtcggcgccg ggccgcgacg acgtgttgcc cgacatcaac gagaatctgg
     2581 tggtggagct gtactcgaag taattccgtt tgcgccggcc gtagccgccg gcaaggtgag
     2641 actgcaatgc agggttccgt gagcgagttt ctgagaccgc gagtggtgaa ggtgcagccg
     2701 gtggccccgc gccaggcgcg cgtggtgatc gagccgttcg agcggggctt cggccacac
//
LOCUS       DATDMP010000482         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_189428, whole genome shotgun sequence.
ACCESSION   DATDMP010000482 DATDMP010000000
VERSION     DATDMP010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189428"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2582)
                     /locus_tag="VI195_08450"
     CDS             complement(<1..2582)
                     /locus_tag="VI195_08450"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY6824456.1"
                     /translation="MLEPTPLDGGNAEFLDALYEQYLRDPGSVGEEWRSYFDRLGAAP
                     GERAHGPLRAAIAQRAMEGRPLGAAAMADNARQASVSRLIQVWINRGHLIADIDPLGL
                     MPRPRPRALDPEYFGLSSADLDAEFFTGSRIEAVPQRLKLREILAQLRAMYAGSIGAE
                     FAHISNSEERLWLQDEFQQGRLRERFTPEEQRGILWQLTAAEGLERYLHTRYVGQKRF
                     SLEGTDALIPLLDDLVQRAGPAGVEEVVIGMAHRGRLNVLVNLLGKSPAELFSEFEGA
                     YDTSRLKGSGDVKYHKGFSADLRTAAGNVHTVLAFNPSHLEVVDPVVEGSVRARQERR
                     GDERAERVLPLLIHGDAAFAGQGVVMETLQLSQARGFFTGGTVHVIVNNQVGFTTSVA
                     ADARSTMYCSDVAKMVEAPIFHVNADDPEAVVFVTRLALKYRLRFRKDVVIDLVGYRR
                     HGHNEADEPAATQPEMYRAIRQRPTARRLYADRLIAAAVLTEAEVESMYEQYRAGLDQ
                     GRPQARASLGMIGNKYTVDWSSFGQVDWTERVASGVDAARLRAVGERITQLPPDFKLH
                     PRVAQLMASRRRMIEGEQPLDWGWAETLAYATLIEDGFSVRLSGQDSGRGTFFHRHAV
                     LHDQNTGAVWIPLQHVAERQPRVQMIDSVLSEEAVMGFEYGYATTEPAALVVWEAQYG
                     DFANGAQVIIDQFISSGEAKWERFCGLTLMLPHGYEGAGPEHSSARLERFLQLCAENN
                     MQVCVPSTPAQMFHMLRRQMHQCFRKPLIVMTPKSLLRHELSVSALEDLSRGAFARVI
                     GEIDELPPAEIRRVVFCSGKVYFDLLRARRKEALRDVALVRIEQLYPFPGEEYQALLN
                     RYPNL"
     gene            complement(2656..3153)
                     /locus_tag="VI195_08455"
     CDS             complement(2656..3153)
                     /locus_tag="VI195_08455"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEY6824457.1"
                     /translation="MKPNNNTPPMEVKEQTAAELVQQRRAPDRRKRVLWSLVYGSFHP
                     RRRGPRREGENQLAAVDWHHPQWLAIGILIVGLSCLDALLTLMLMARGAYEMNPLMAV
                     LIGGSVTRFAYVKISLTAVGVVLLTQLSRLRTFGRIPVGLILYSLLVLYGTLIFYEFQ
                     LLDRL"
     gene            3329..>3882
                     /locus_tag="VI195_08460"
     CDS             3329..>3882
                     /locus_tag="VI195_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824458.1"
                     /translation="MSASSAAAATAAHPDGLPVPRRYWAIAAIALAITMSVLDSTIVN
                     VALPTIARDFQASSAASIWVINAYQLAILLVLLPFASLGEIVGYRRVSQAGLALFTLA
                     SLGCAFAPNLLALSLARVIQGLGAAGIMSVNGALVRHTYPHRYLGRAVGINAFVVASA
                     AAVGPTVASGVLAVAQWRWLFGINV"
BASE COUNT          643 a         1295 c         1286 g          658 t
ORIGIN      
        1 aggttggggt agcggttgag cagcgcctgg tactcctcac ccgggaaggg atagagctgc
       61 tcgatacgca cgagcgcgac gtcgcgcagg gcttccttgc gccgggcgcg cagcagatcg
      121 aagtaaacct taccgctgca gaacacgaca cggcggatct ctgccggcgg cagctcatcg
      181 atctcaccga tcacgcgcgc gaaagcgccg cggctgaggt cctcgagcgc cgacaccgac
      241 agctcatggc gcagcaggct cttcggcgtc atgacgatca gcggcttgcg gaaacactgg
      301 tgcatctgcc gccgcagcat gtgaaacatc tgcgccgggg tggagggcac gcacacttgc
      361 atgttgttct cggcgcacag ctgcaggaag cgctcgaggc gcgccgagga gtgctccggt
      421 cctgcgccct cgtagccgtg cggcagcatg agcgtcagcc cgcagaagcg ctcccacttg
      481 gcctcacccg agctgatgaa ttgatcgatg atgacctgcg cgccgttcgc gaaatcgccg
      541 tattgcgctt cccagaccac gagcgccgcg ggctcggtgg tggcgtagcc gtactcgaag
      601 cccatgaccg cctcttccga gagtaccgag tcgatcatct gcacgcgcgg ctgccgctcg
      661 gctacgtgct gcaatgggat ccagacagcg ccggtattct gatcgtgcag caccgcgtgg
      721 cgatggaaaa aggtgccgcg gccactgtcc tggccgctga ggcgcacgct gaagccatcc
      781 tcgatgagcg tcgcgtaggc gagcgtttct gcccagcccc aatcgagcgg ctgctcaccc
      841 tcgatcatgc gacggcggct cgccatgagc tgcgccacgc gtggatgcag cttgaaatcc
      901 ggcggcagct gggtgatgcg ctcaccgacc gcgcgcagcc gcgccgcgtc gactccgctt
      961 gcgacgcgct cggtccagtc cacctgccca aaggagctcc agtcgacggt gtacttgttg
     1021 ccgatcatgc cgagcgaggc gcgcgcctgc ggccgccctt gatcgagccc ggcacggtac
     1081 tgctcgtaca tcgactcgac ctctgcctcg gtcagcactg cggcagcgat caagcggtcg
     1141 gcgtacagcc gccgcgcggt cggtcgctgg cggatcgcgc ggtacatctc cggctgggtc
     1201 gcggccggct catccgcctc gttgtggccg tgtcggcggt agccgaccag gtcgatcact
     1261 acgtccttgc ggaatcgcag gcggtatttg agcgcgagcc gtgtgacgaa cacgacggcc
     1321 tcgggatcgt cggcattgac gtgaaaaatc ggcgcctcga ccatcttggc gacatcgctg
     1381 caatacatgg tcgagcgcgc gtccgccgcg acggaggtgg tgaagcccac ctggttgttg
     1441 acgattacgt gcacggtgcc gccggtgaag aagccgcgcg cctgcgacag ctgcagcgtc
     1501 tccatcacca cgccctggcc cgcgaaggcg gcatcgccgt ggatgagtaa cggcagcacc
     1561 cgctcggcgc gctcatcgcc acgccgttcc tgacgggcac gcaccgagcc ctcgaccacc
     1621 ggatcgacga cctcgagatg cgaagggttg aacgccagca ccgtgtgcac gttgcccgcc
     1681 gcggtgcgca ggtctgcgga gaagcctttg tggtatttga catcgcccga gcctttcaac
     1741 cggctcgtgt cgtaggcgcc ttcgaattcc gagaacagct ccgccgggga cttgccaagc
     1801 aggttcacca gcacgttcag ccgcccgcgg tgcgccatgc cgatgaccac ctcctcgaca
     1861 ccggcagggc cggcgcgctg caccaggtca tcgaggagcg gaatgagcgc gtcggtaccc
     1921 tcgagcgaga agcgcttctg accgacataa cgggtgtgca ggtagcgctc gaggccctcg
     1981 gcggctgtca actgccagag aatgccgcgt tgctcttcgg gcgtgaaacg ctcgcgcagg
     2041 cgcccctgtt ggaattcatc ctgcagccac agccgctcct cggagttgga gatgtgcgcg
     2101 aactccgcgc cgatgctgcc ggcgtacatc gcgcgcagtt gcgcgagaat ctcgcgcagc
     2161 ttgagccgtt gcggtaccgc ctcgatacgg ctgccggtga agaactccgc atcgagatcg
     2221 gcgctgctga ggccgaagta ctccggatcg agcgcgcgcg ggcgcggccg cggcatgagt
     2281 ccgagcgggt cgatgtccgc gatcagatgc ccgcggttga tccacacctg tatgagccgc
     2341 gacacggaag cctggcgcgc gttgtccgcc atagcggccg caccgagcgg acggccttcc
     2401 atggcgcgct gcgcgatggc cgcgcgcagc gggccgtgcg cacgctcgcc gggcgcggca
     2461 ccgagccggt caaagtaact gcgccactcc tcgccgacgc tccccggatc gcgcagatac
     2521 tgctcgtaga gggcatcgag gaactcggcg ttgccgccgt cgagtggtgt gggttcgagc
     2581 atgggaaaac tgccggcagg ccagtagttt agcggaaaag gccggaggcc gagaaaggcg
     2641 gccggcgcag cggtgttaca gacggtcgag caactggaat tcgtagaaaa tcaacgttcc
     2701 gtagagcacg agcagcgagt agaggatgag ccccaccggt atgcggccga aggtgcgcag
     2761 ccgcgagagt tgtgtgagca gcactacacc cactgccgtc aggctgatct tcacgtaggc
     2821 aaagcgcgtc accgaaccgc cgatgagcac cgccatcagg gggttcatct cgtaggcgcc
     2881 gcgtgccatc agcatcagcg tgaggagcgc atcgaggcac gacaacccta cgatcagtat
     2941 cccgatggcg agccactgcg ggtgatgcca gtcgactgcg gccagctggt tttcgccctc
     3001 acgccgcggg ccgcggcgcc gcggatgaaa actgccatac acgagcgacc acagcacgcg
     3061 cttgcgccga tcaggcgcgc gccgctgttg caccagctca gcggcggtct gctctttcac
     3121 ttccatgggt ggggtgttgt tgttgggttt caagtggcgc tgcccgcatt gtagcggacg
     3181 ccgccacggg cgaggacgtg atgcgactca cgggggccgc ctcgatccag caaaagacct
     3241 gccgcacagc gctggcgcag ccagtcgtgc ctccccgcgc cacgttatga cagcgccccc
     3301 gggcgcacct tccttatatg atggggtggt gtctgcctcg agcgccgctg ccgcaaccgc
     3361 tgcccatccc gacggactgc cggtaccgcg acgctactgg gcgatagcgg cgattgcgct
     3421 cgcgatcacg atgtcggtgc tggacagcac cattgtgaat gtcgccctgc cgaccatcgc
     3481 gcgcgatttc caggcatcct cggcggcctc catctgggtc atcaatgcct atcagctcgc
     3541 cattctcctg gtgctgctgc cgtttgcatc gctcggggag atcgtgggct accggcgggt
     3601 gtcgcaggcc gggctcgctc tgttcaccct cgcctctctc ggctgcgcat ttgcgccgaa
     3661 tctgctggcg ctgagtctcg cgcgcgtgat tcagggactc ggggcggccg gcatcatgag
     3721 cgtcaacggt gcgctggtgc gacacactta tccgcatcgc tacctcggcc gcgcggtcgg
     3781 cattaacgcc ttcgtggtcg caagcgctgc cgcggtcggg ccgacggtcg cctccggcgt
     3841 gctcgccgtg gcccagtggc gctggctgtt cggcatcaac gt
//
LOCUS       DATDMP010000483         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1744873, whole genome shotgun sequence.
ACCESSION   DATDMP010000483 DATDMP010000000
VERSION     DATDMP010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744873"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..561)
                     /gene="greB"
                     /locus_tag="VI195_08465"
     CDS             complement(61..561)
                     /gene="greB"
                     /locus_tag="VI195_08465"
                     /inference="COORDINATES: protein motif:HMM:NF002506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY6824459.1"
                     /translation="MGRFRLPPPTASKYVTPAGARRLRAELEQLWREERPRVTQAVAA
                     AAAQGDRSENAEYTYGKRRLREIDRRVRFLRRRLEGMVVVDQPPADPGRVYFGAWVGI
                     ETEDGLQARYRIVGPDEFDMAPGYISMDSPLGQALMRRALDEEVSVAAPGGSRSYVIV
                     AIEYEP"
     gene            665..868
                     /locus_tag="VI195_08470"
     CDS             665..868
                     /locus_tag="VI195_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824460.1"
                     /translation="MATKYYTHFVTHAGETRNAARNEWSGVVELRQPLTQARANRELR
                     ALLAQSFDLDSDEIRILHWARLH"
     gene            1036..1686
                     /locus_tag="VI195_08475"
     CDS             1036..1686
                     /locus_tag="VI195_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY6824461.1"
                     /translation="MRAWSVALVAFAVFTLSGCGYNTLQAKDEGVKAAWAEVVNQYQR
                     RADLIPNLVNTVKGYAAQEQRVLVEVTEARAKVGSIQVTPEVLNNPELFQKYQAAQNQ
                     LTGALKSLFAVAENYPQLKSDQNFRDLQSQLEGTENRITVARNRYIQAVQEFNVTVRS
                     FPTNLTAKLFGFQVKPNFSVANEPQISTAPTVSFDTSTPGSSAAPAPPPAGSPPKQ"
     gene            1683..2621
                     /locus_tag="VI195_08480"
     CDS             1683..2621
                     /locus_tag="VI195_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEY6824462.1"
                     /translation="MTSRAHGAAHCARLAALLLVSLGIDAHAQGPVSGLQPVPPLTAR
                     VTDLTGTLTAEQQTALEQKLAAFEARKGSQLAVLIVPTTHPEEIEQYSIRVVDKWKLG
                     RGTVGGKKVDDGALLLIARDDHRIRIEVGYGLEGVLTDAMSNRIISETIAPAFREGNF
                     YEGVDAGVGQMMKLIEGEPLPPPEHAWQSRRHTSGGNILPELLFAVLVGSVVLRGIFG
                     RTLGSVFTGLGAGALVWIAGHALILAVAAAIGGFLLTLLMGLPRGSGWSSYPRSGGFG
                     GGWGGFGGGMGGGGFGGGGFSGGGGGFGGGGSSGSW"
     gene            2628..>3096
                     /locus_tag="VI195_08485"
     CDS             2628..>3096
                     /locus_tag="VI195_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY6824463.1"
                     /translation="MNILRLVRHIGTTRWDTRRHFPQAARDAIEQAIGQCESLHGGEI
                     RFVIETALDMPELWRDLSSRTRALQLFGQFGVWNTEHNNGVLIYVLMADHAVEIVADR
                     GIAARVEQAEWDAVCRQMEHHFRAGRFRDGAVVGILGVGALLGRHFPGRRRSDN"
BASE COUNT          514 a         1012 c         1035 g          535 t
ORIGIN      
        1 cgatccctgg cagttcaaga acgtgcgcgt catctggtga ggtaccggcg cgggtagcct
       61 tcagggctcg tactcgatcg ccacgatcac atagctacgg ctgccgcccg gggccgccac
      121 gctcacttcc tcatcgagcg cgcgccgcat cagcgcctgt cccagcggtg agtccatgct
      181 gatgtagccg ggtgccatgt cgaactcgtc cggcccgacg atgcggtagc gcgcctgcag
      241 gccatcctcg gtctcgatcc ccacccaggc cccgaaatac acccgcccgg gatccgccgg
      301 cggctggtcc acgaccacca tgccctccag gcggcggcgt agaaaccgca cccggcggtc
      361 gatctcgcgc aggcgccgct tgccgtaggt gtattcggca ttctcggagc gatcgccctg
      421 ggcggctgcg gcggcaaccg cctgtgtgac ccggggccgc tcctcgcgcc agagctgctc
      481 gagctcagcc cgcaggcgcc gtgccccggc gggcgtgacg tatttcgagg cggtgggcgg
      541 gggcagccgg aagcgtccca tgcgctcgat ttccgtggat tatgtcgcac tcacttttac
      601 agaaatccgg ggttctgtgg aacatctcac ggacctgcac cgggcaggct actaaccttt
      661 tgccgtggct accaagtact acacgcactt cgtgacccac gccggcgaga cacgtaacgc
      721 cgcccgcaac gagtggagcg gtgtggtgga gctgcggcag cccctgacac aggcgcgcgc
      781 gaatcgtgag ctgcgcgcgc tgctcgcgca gagctttgac ctggactcgg acgagatccg
      841 cattctgcat tgggctcgat tgcactgagg tatcccctgg cggacttcct tcccttcatc
      901 gaaggtccgc ccttaccaac cccaggcctc aaaacctggg gttttttttg cgcgatcgac
      961 gccgctttcg cggccatgtg acaataatct gcgcccccgg gagccggacg ggcgctacac
     1021 gataaggaga ggccgatgcg cgcttggtca gttgccctgg tggcattcgc agtgttcacc
     1081 ctgtcgggct gcggttacaa caccctgcag gccaaggatg aaggggtgaa agcggcctgg
     1141 gcggaggtgg tcaaccagta ccagcgtcgt gcggacctca tccccaacct ggtgaacacg
     1201 gtgaaggggt acgccgccca ggagcagcgc gtgctcgtcg aggtcaccga ggcgcgtgcc
     1261 aaagtcggct cgattcaggt gacgccggag gtgctcaaca accccgagct cttccagaag
     1321 taccaggccg cgcagaatca gctcaccggc gcgctgaaga gcctgttcgc ggtggcagag
     1381 aattatccgc agctgaagtc ggaccagaac ttccgcgacc tgcaatcgca gctcgagggc
     1441 acggagaacc gcatcacggt cgcgcgcaac cgctatatcc aggcggtgca ggagttcaac
     1501 gtgaccgtgc gcagcttccc gaccaatctc accgccaagc tgttcggctt ccaggtgaag
     1561 ccgaacttca gcgtcgccaa cgagccgcag atctccaccg cgccgacggt gagcttcgat
     1621 acctccaccc cgggctcatc ggccgcgcca gcgccgccac ctgcgggatc gcccccgaag
     1681 caatgaccag ccgggcgcat ggcgccgccc actgtgcgcg cctggcggcg ctgttgctgg
     1741 tgagcttggg tatcgatgcg cacgcacagg ggccggtgtc agggctgcag ccggtaccgc
     1801 cgctcactgc gcgtgtcacc gatctcaccg gcacgctcac ggcggagcag cagaccgcgc
     1861 tcgagcaaaa gctcgccgcc tttgaggcac gcaagggctc acaactcgcg gtgctcatcg
     1921 taccgacgac gcacccggag gagatcgagc agtactcgat acgggtggtc gacaagtgga
     1981 aactcgggcg cggaaccgtc ggtggcaaga aggtcgatga cggcgccctg ctgctcatcg
     2041 ccagggacga ccatcgtatt cgcatcgagg tgggctacgg cctcgagggg gtactcacgg
     2101 acgcgatgtc caaccgcatc atctccgaga cgatcgcgcc tgctttccgt gaaggcaact
     2161 tctacgaggg cgtcgatgcc ggcgtcgggc agatgatgaa gctcatcgag ggcgagccgc
     2221 tgccgccgcc ggagcatgcc tggcagagtc gccggcacac ttccggcggc aacatccttc
     2281 ccgagctgct gtttgccgtg ctcgtcggat cggtggtgtt gcgcggcatc ttcggccgca
     2341 cgctcggatc ggtatttacc ggcctcggtg cgggcgcatt agtgtggatt gccggccatg
     2401 cgctcatcct cgccgtggcg gcggcgatcg gcgggtttct gctcacgcta ctaatgggtc
     2461 tgccgcgcgg cagcggctgg tcgagctatc cgcgctcggg cggttttggc ggcggctggg
     2521 gcggttttgg cggtggaatg ggcggcggtg gtttcggcgg gggcggcttc agcggaggag
     2581 gcggcggatt cggcggtggc ggctcctccg gaagctggta gcagtcgatg aacatcctgc
     2641 ggctcgtgcg tcacatcggc accactcgct gggatacacg gcggcacttt cctcaggccg
     2701 cgcgtgacgc catcgagcag gcgatcgggc agtgtgaatc gctccacgga ggggagatcc
     2761 gcttcgtcat cgagaccgcc ctcgatatgc cggagctgtg gcgcgatctc tcatcccgca
     2821 cccgcgcatt gcagctgttc ggacaattcg gcgtctggaa caccgagcac aacaatgggg
     2881 tgctgatcta tgtgctcatg gccgatcatg cggtcgagat cgtcgccgac cgtgggatcg
     2941 cggcccgcgt cgagcaggcg gaatgggacg cggtgtgccg ccagatggag catcacttcc
     3001 gagccggccg tttccgggac ggcgcggtgg taggcatcct gggggtggga gcgcttctcg
     3061 gacggcactt cccgggcaga cgccgcagcg acaatg
//
LOCUS       DATDMP010000484         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_411859, whole genome shotgun sequence.
ACCESSION   DATDMP010000484 DATDMP010000000
VERSION     DATDMP010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411859"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VI195_08490"
     CDS             <1..526
                     /locus_tag="VI195_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY6824464.1"
                     /translation="HVDAHLDWRVDVGGVRRGYSSPLRWASTKRWIAGMTQIGMRSTG
                     SARRGEVEDARRYGSHIFTAGDIHAQGMDPVLSSIPPAVPVYVTIDADGMDPTEMPAV
                     MAPAPGGVRFEQLAPLLRALAMRQRIVGLDIVEIAPQFDQANAITAVTAGRLILNALG
                     ASWGDGGAYRGGQH"
     gene            556..1854
                     /locus_tag="VI195_08495"
     CDS             556..1854
                     /locus_tag="VI195_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824465.1"
                     /translation="MIATGLRHQESDGESLSLASVNREQWRAFWAVFLGWLVDAFDFN
                     MLAFILIDIQKSFTVDRALAGALGSITLLMRLLGSIGAGVLADRYGRKLPLILSILWF
                     SVFAFLSGFSTSYAMLFALRALFGIGMGGEWAAGMPLVLEHWPARLRGLASGLLQGGW
                     YWGYLLAAVVFQFVYPLFGATPGLGWRVMLWAAIVPAMLALWVRRRVKESPVWLERRR
                     LITGAAPQRLAPTAISLVRIFERDLIGSTLQTAALIASFMCMYYAINFWYPTLLRDSG
                     HAPLPYLAAINIGAIVGTAAWGRISETTLGRRGAVTLSILLAAAVLPLYLYARAPWWM
                     AVGALSMGAFGNGVWGTAPAYTAERFPTAVRGVGPGFCYHCGAAIGALMPWLVGRLQD
                     RGTPIATAIGSAMLVSGAIALGFIWLGPETRGRVFVAESS"
     gene            1854..2747
                     /locus_tag="VI195_08500"
     CDS             1854..2747
                     /locus_tag="VI195_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6824466.1"
                     /translation="MDDPVYVLGHSEQELQRLRRQAQLIAPATRLFLCEGGLGAGMRV
                     LDVGSGAGDVAFLAAGLVGETGEIIGTDKSPAAVAAANARARAQGLRHVSFHEGDPAY
                     LSFERSFDAVIGRYVLLFQSDASAMLRQLLRHLRGGGLVVFHEPDWHSARSIPSVPTY
                     DRCCRWVQQTFARAGTDTNMAGRLYQALVEAGLSNPTLRMQTFIGGGVQCADYLQAVA
                     DLVGSMLPAMEQHGVASAAEVDYPTLAVRMQQETMSRKSLIIGRSEIGAWARLPADRA
                     DARSARQIEASAKQKSDRQGR"
     gene            2750..>3109
                     /locus_tag="VI195_08505"
     CDS             2750..>3109
                     /locus_tag="VI195_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY6824467.1"
                     /translation="MLTTDEIRAVPLFSALDLNGLEQLARTSEDLHLKAGEFAVHEGG
                     ERALFAVLAGKLEVIKLIDGVERRLGWRIPGTIFGEVPIALGAPFPAAYRAVETSRVM
                     RMDSRHYYAIAAAAPEVA"
BASE COUNT          486 a          953 c         1067 g          603 t
ORIGIN      
        1 gcacgtcgat gctcacctcg actggcgcgt ggatgtcgga ggtgtgcggc gcggctattc
       61 gagtcctttg cgctgggcca gcaccaagcg ctggatcgcc ggtatgaccc agattggcat
      121 gcgttccacc ggaagcgcac ggcgcggcga ggtggaggac gcacgccggt acggttcgca
      181 catcttcacc gccggggaca ttcatgccca gggcatggat ccggtgctct cgagcattcc
      241 gccggcggtg ccggtgtacg tcaccatcga tgcggacgga atggatccca ccgagatgcc
      301 ggccgtcatg gctccggctc ccgggggtgt gcgtttcgag caacttgccc cgctgctgcg
      361 cgcgctggcg atgcgtcaac gcatcgtggg actggacatc gtggagatcg cgccacagtt
      421 cgatcaggcc aacgccatta ccgcggtgac ggccggacga ttgatcctca atgctctggg
      481 cgcgtcgtgg ggtgacggcg gggcatatcg aggcgggcag cactgattca gagtggcagc
      541 gcaaaagttc agcgcgtgat agcgactggc ctccgccacc aagagagcga cggcgaatcg
      601 ctgtcgctgg catcggtgaa ccgggaacaa tggcgtgcgt tctgggccgt gttcctcggc
      661 tggctggtcg atgcattcga cttcaacatg ctggccttca tcctgatcga cattcaaaag
      721 agcttcacgg tcgaccgcgc gctcgcgggc gcactcggca gcattactct gctgatgcgg
      781 ctgctcggta gcattggcgc gggtgtattg gccgacaggt atggccgcaa gctgccgctg
      841 atattgtcga tcctgtggtt ctcggtgttc gcgttcctca gtggcttctc gacctcttac
      901 gcaatgctgt tcgcgctgcg agcgttgttc gggataggca tgggcgggga gtgggcggcg
      961 ggcatgccgc tggtgctcga gcactggccg gcgcggctgc gcggcctggc atcggggctg
     1021 ttgcagggcg gctggtactg gggctacctg ctggctgcgg tcgtattcca gttcgtatat
     1081 ccgctctttg gcgcgacacc gggtctcggc tggcgcgtga tgttatgggc tgcgatcgtg
     1141 ccggcaatgc tggcgctttg ggtcaggcgc cgggtgaagg aaagcccggt ctggctggag
     1201 cgacgccgcc tgatcacggg tgctgcgccg cagcgcctag cgccgaccgc gatctcgctg
     1261 gtgcgcatct tcgagcgcga cttgatcggc tcgaccctgc agaccgcggc gctcatcgcg
     1321 tcgttcatgt gtatgtacta tgcgatcaac ttctggtacc cgacactgct gcgagattcg
     1381 ggacacgcgc ccctgccgta cctggcggcg atcaacatcg gagcgatcgt cggcactgcc
     1441 gcatggggac ggatctccga gaccacgctc ggccgtcgtg gcgccgtgac actgtcgatt
     1501 ctgttggcag cggccgtgct gccgctttat ctgtatgcac gcgcgccctg gtggatggca
     1561 gtaggcgcac tatcgatggg tgcatttggt aacggcgtat ggggcacggc acctgcctat
     1621 accgcagagc gcttcccgac cgcggtgcgc ggtgtgggcc caggattctg ttaccactgc
     1681 ggtgcggcca tcggcgcact gatgccttgg ttggtcggca gactgcagga ccgcggcacg
     1741 ccgatcgcca ccgccatcgg tagcgcgatg ctcgtgtcgg gcgcgatcgc actggggttc
     1801 atctggctcg ggcccgagac gcgcggccgg gtattcgtcg cggaatcgag ctgatggacg
     1861 atcccgttta cgtgctcggc cattccgagc aggaattaca acgcctgcgc cggcaggcgc
     1921 agctgatcgc gccggcgacc cggctatttc tgtgcgaagg cggactgggc gccggcatgc
     1981 gcgtgctgga tgtcggcagc ggagcgggcg acgtggcgtt tctcgccgcc gggcttgtgg
     2041 gcgagacggg cgagatcatc ggcacggaca agtcgccggc cgcagtcgcc gcggcaaacg
     2101 cacgcgcgcg ggcgcagggt ctacgtcacg tcagtttcca cgagggcgat cccgcttacc
     2161 tgagtttcga gcggtcgttc gatgccgtca ttggcaggta cgtgctgctg tttcagtcag
     2221 atgcctcggc catgttgcgc caactcctgc ggcacctgcg tggcggtgga ttggtggtgt
     2281 tccacgagcc cgactggcac agcgcgcgct cgataccctc ggttccgacc tacgatcgct
     2341 gttgccgatg ggtccagcag accttcgccc gcgcaggcac cgacacgaac atggcgggca
     2401 ggttatacca ggcgctggtc gaggcgggtt tgtcaaaccc aacgttgcga atgcagacct
     2461 tcatcggcgg tggcgtccaa tgcgcggact atctgcaagc cgtcgctgac ctggtcggca
     2521 gcatgctgcc cgcaatggaa cagcatggcg tggcgagcgc cgcagaggtc gattacccga
     2581 ccctcgccgt gcgcatgcag caggaaacga tgagccgcaa atccttgatc atcggtcgct
     2641 ccgagatcgg cgcctgggcc cggttgccgg cggaccgggc tgacgcccgt tccgcgcgcc
     2701 agatcgaggc atcggccaag caaaaaagcg atcggcaggg gcggtaacga tgctgaccac
     2761 agatgagatc agagctgtcc cgctgttttc ggcgctcgac ctgaacggtc tcgagcaact
     2821 cgcgcgtacc tctgaagatc tgcacctcaa agccggtgaa ttcgctgttc acgagggcgg
     2881 agagcgagca ctctttgcag ttctcgccgg caagctcgaa gtcatcaagt tgatcgatgg
     2941 agtggagcgc cgattgggtt ggcgcatccc cggcaccatt ttcggcgaag tacccattgc
     3001 gcttggtgcg ccgtttccgg ccgcctatcg ggcggtagaa acctcgcggg tcatgcgcat
     3061 ggactctcga cattactacg ccatcgccgc ggctgcgccg gaggtcgcc
//
LOCUS       DATDMP010000485         4891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_522412, whole genome shotgun sequence.
ACCESSION   DATDMP010000485 DATDMP010000000
VERSION     DATDMP010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522412"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VI195_08510"
     CDS             <1..1350
                     /locus_tag="VI195_08510"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY6824468.1"
                     /translation="TVEVTATSALLNARADLGDGAVGTIHATLELERAPRWQDMPLNG
                     ELHLQTSEANLVTLYVPDIDRAVGHLGADLRLAGTAGTPQVTGSVKVSDGELDVYQIN
                     LSLRRLAMQAQLSDSGVDFKGSAQAGKGEVSANGHLEWRRLLPYGKFHLEGSNLRVAD
                     LPEAQIDASPALDFDVSGRKIEVTGKVTIPFAKIQPKDITGAVRTSPDEVIVGSEPED
                     ARERFEVLSTITLTLGDKVNVDALGLTARLIGSVTIRNGYDPITRGTGELSVAEGKYA
                     AYARQLDIQRGRLIFTGGPITNPGIDVVAQKVFPDVTAGVNVRGTLAQPHISFFSDPP
                     LPQQQVASLILAGGSLESSQNASNAALGQGAALLAAQLGSHVGIPDVSLETDPIVNET
                     SLVLGRYLSPRLYVSYGVSLTEQLNTLKMRYTLGDHWTVRTEVGQAYGADLVYSITK"
     gene            1353..2138
                     /locus_tag="VI195_08515"
     CDS             1353..2138
                     /locus_tag="VI195_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824469.1"
                     /translation="MLVTATVVLLALLLAPAAAGAAHPPAAPSCQSFSREGQSPPPLV
                     RAWFADSADQRVVVCPQSATAAGAGPVPLYFGEGAVSQHGEVCSYLSHGLTLIGSGAS
                     ARLQRYERSEALDMALTGAAGCPQPHTAAGTPAYVETYDTTPAAFEGIMRLWSAATAA
                     LALPANEHCCAGRAAAAQSGAAAGRIAPEAREHIEAALGADHTSTATVTRIVRIPGSV
                     LRHRYALFLTVTGTAGGSAALYVVYVDKPLRGTYEITAFAETN"
     gene            2296..2784
                     /locus_tag="VI195_08520"
     CDS             2296..2784
                     /locus_tag="VI195_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824470.1"
                     /translation="MHLYAEAEGIPVMLSDAEQLERRGAIMRILRQGQVRRQQDLVRL
                     LKSAGHAVTQSSISRDLRDLGVLKASGRYLLPPEEVTRTNGDFGTLAQFVRELRLAGP
                     AITVLRTTIGAAQSVAVAIDRAAWPEVAGTLSGDDTIFIATANARAQDKLIERLRALF
                     PL"
     gene            2787..4034
                     /gene="argG"
                     /locus_tag="VI195_08525"
     CDS             2787..4034
                     /gene="argG"
                     /locus_tag="VI195_08525"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY6824471.1"
                     /translation="MTHTAAAPGAPIVLAYSGGLDTSFLVPWLAEHERRPVITVTVNT
                     GGIDAAAAGTLAERARALGAAAHRQVDARSGYFEQVLRFLIMGNVRRGQLYPLCVGAE
                     RVVQAQTLARVAMELGSDTVAHGCTGAGNDQVRFEVALRTLAPDLKVLAPVRDQAFRR
                     EYELGFLRERSLPGPPSGARYSVNRGLWGVTIGGRETLSSAGSLPDEAWILTRDAFSS
                     LRTPERHTLSFSEGRPVALDGKSCDSVALIEALEALAAPFGIGRGIHLGDTIIGTKGR
                     VAFEAPAAELLLTAHRELEKLTLTARQQRVKELLALPYGDLVHEGQLLDPVCRDIEAL
                     LSSSQQRVTGEVRLLLRTGSLFVEGVESPHSLMAASRGVYGEAAGEWSARDALGFSRI
                     VALPGVFHARAAAHGGGTPPGER"
     gene            4037..>4891
                     /locus_tag="VI195_08530"
     CDS             4037..>4891
                     /locus_tag="VI195_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824472.1"
                     /translation="MVSMRNVVVDKLASVTQACGLSHEVRISADIPAEEGVVIVVEVL
                     NNKSTYNTLELVSGRMAKVGKGDIVAGALGHRQALFGYSGHVPPALQPGDVIQMLNIG
                     GVLGVCDSINPEKGRPFDCRVLGVALNFPYLGERIGVPARVGHKPLDAAAPLEVRGVP
                     VVALAGTCMEAGKTAAACAVIARMRHRGLTVDAFKATGVSLRRDILAMEDAGARRCSI
                     FTDLGVVSTTRANGPALTRAMLSELAAGKPDVLVFELGDGMLGTYGVDAILECPDIRA
                     ALTAVILSA"
BASE COUNT          741 a         1756 c         1648 g          746 t
ORIGIN      
        1 accgtggagg tgaccgccac gtccgccctc ctcaatgcac gcgccgatct cggcgatggt
       61 gcggtcggga cgatacatgc gacgctcgag ctggagcgcg caccgcgttg gcaggacatg
      121 cccttgaacg gcgagctcca cctgcagact tccgaggcca acctggtcac tctgtatgtg
      181 cccgatatcg accgtgcagt cggccacctc ggcgccgacc tgcgacttgc aggcaccgca
      241 ggcacgcccc aggtgaccgg ctcggtcaag gtgagcgacg gcgagctcga tgtctaccag
      301 atcaatctca gcctgcgtcg gctcgccatg caggcgcagc tttccgacag cggcgtcgat
      361 ttcaagggca gcgcgcaggc gggcaagggc gaggtgagcg ccaacggtca tctcgagtgg
      421 cgccggctcc tgccctacgg caagttccac ctggagggca gcaacctgcg cgtggcagat
      481 ctgccggagg cgcagatcga cgcgtccccg gcgctcgact tcgatgtcag cggacgcaag
      541 atcgaggtca ccggcaaggt caccataccg tttgcgaaga tccagcccaa ggacatcacc
      601 ggcgcggtcc gcacctcacc cgatgaggtg atcgtcggca gcgagccgga ggacgcgcgc
      661 gagcgcttcg aggtgctgag caccatcacg ctcaccctcg gcgataaggt caacgtcgat
      721 gcgctcgggc tcaccgcgcg tctcatcggc agcgtcacga tccgcaacgg ctacgacccc
      781 atcactcgcg ggacgggcga gctgtcggtg gcggagggca agtatgccgc ctacgcgcgc
      841 cagctcgata tccagcgcgg ccgcctgatc ttcaccggcg gaccgatcac caacccgggc
      901 atcgatgtcg tcgcacagaa ggtgtttccc gatgtcaccg cgggcgtcaa cgtgcgcggc
      961 acgctggcgc agccacacat ctcattcttc tccgatccgc cgctgccaca gcagcaggtc
     1021 gcctcgctca ttctcgccgg cggctcgctc gagtcgtcgc agaacgccag caacgccgcg
     1081 ctcggccagg gagcggcgct gctcgccgcg caactcggct cgcacgtggg cattccggat
     1141 gtgagtctcg agaccgaccc gatcgtcaac gagacctcgc tcgtgcttgg ccgttacctc
     1201 tccccacggc tgtacgtaag ttacggcgtg agcctcaccg agcagctcaa cactctcaag
     1261 atgcgttata cgctcggcga ccactggacg gtgcgcaccg aggtgggtca ggcttacgga
     1321 gcggatctgg tgtactcgat cacgaagtag gagtgctcgt gaccgcgacc gtggtgctct
     1381 tggcgctgct gctggcaccg gctgctgcag gcgcagcgca cccacccgcc gcaccctcct
     1441 gccagagctt cagccgcgag gggcagtcgc caccgccgct ggtgcgtgca tggttcgcgg
     1501 actcggccga tcagcgcgtc gtggtgtgcc cgcaaagcgc gaccgcagcc ggtgcggggc
     1561 cggtgccgct ttacttcggc gagggtgcgg tgagccaaca cggcgaggtg tgcagctacc
     1621 tcagccacgg cctcacgctc atcggtagcg gtgccagcgc ccggctgcaa cgttacgagc
     1681 gcagcgaggc actggacatg gcactgacgg gcgccgccgg ttgcccgcag ccgcacactg
     1741 cggcgggtac gcccgcgtat gtcgagacct acgatacaac cccggcggcg ttcgagggca
     1801 tcatgcggct ctggtcggcg gcgaccgccg ctctcgccct gcccgcgaac gagcactgct
     1861 gcgccgggcg cgctgcggcg gcgcagtccg gcgccgctgc cggcaggatc gcgccggagg
     1921 cgcgcgagca tatcgaggcc gcgctcggcg cggaccacac atcgaccgca accgtcacgc
     1981 gcatcgtgcg cattcccggt tcagtgctgc gccaccgcta cgcactattt ctcaccgtga
     2041 ccggcacggc cggcggcagc gcggcactgt acgtggtcta cgtcgacaag ccgctgcgcg
     2101 gcacgtacga gatcacggcc ttcgccgaaa ccaactgagt cgcccactcg cgcggtcgac
     2161 gcgcgcacct cctgcggagc gcggagcagc agctgcggga tcggtcctcg ccgacctgta
     2221 tcaacatgtt gatagccgcg ccagtgcgtt gcgccatgcg caccttgacc gcttctgcat
     2281 gaatatgcac aattcatgca tctttatgcg gaagcggaag ggatcccggt catgctgagc
     2341 gatgccgagc agctcgagcg gcgcggcgcc atcatgcgca tcctgcggca gggccaggtg
     2401 cggcggcagc aggatctggt gcggctgctg aagagcgccg gccatgccgt cacccagtcc
     2461 tcgatcagcc gcgacctgcg cgacctcggg gtcctcaagg cgagtggccg ctacctgctg
     2521 ccgcccgagg aggtgacgcg caccaacggc gatttcggca cgctcgcgca gttcgtgcgc
     2581 gagctgcgcc tcgccggccc tgctatcacc gtgctgcgca ccaccatcgg cgccgcgcag
     2641 agcgtcgcgg tcgccatcga ccgtgccgcc tggccggaag tggcgggcac gctctccggc
     2701 gacgacacca tcttcatcgc caccgccaac gcccgtgcgc aggacaagct catcgagcgg
     2761 ctgcgcgcac tatttccact ctgaccatga cccacaccgc tgccgccccg ggcgcgccca
     2821 tcgtgctcgc ctactcggga ggtctcgaca cctccttcct cgttccctgg ctcgccgagc
     2881 acgagcgccg cccggtcatc acggtcaccg tgaacaccgg cggcatcgat gcggccgcgg
     2941 ccggcacgct cgcggagcgc gcgcgcgcac tcggcgccgc cgcgcacagg caagtggatg
     3001 cgcgcagcgg ctacttcgag caggtgctgc ggtttctcat catgggcaat gtacgccgcg
     3061 ggcagctcta tccgctgtgc gtcggcgccg agcgcgtcgt gcaggcgcag accctggcgc
     3121 gtgtagccat ggagctcggc agcgacacgg tcgcccacgg ctgcaccggc gcgggcaacg
     3181 accaggtgcg cttcgaggtc gcgctgcgca cgctcgctcc cgatctgaag gtgctcgcac
     3241 cggtgcgcga tcaggccttc cgccgcgagt acgagctcgg cttcctgcgc gagcgctcgc
     3301 tgccaggtcc gccgagcggc gcgcgctact cggtgaaccg cgggctgtgg ggcgtcacca
     3361 tcggcggccg cgagacgctc agctccgccg gcagcctgcc ggatgaggca tggatcctca
     3421 cccgcgatgc gttctcgagc ctgcgcactc ccgagcgcca cacgctcagc ttcagcgagg
     3481 ggcgtccggt cgcactcgat ggcaagagtt gcgattcggt ggcactcatc gaggctctcg
     3541 aagcgctcgc cgcacccttc ggcatcggcc gcggcatcca tctcggcgac accatcatcg
     3601 gcaccaaggg acgggtggcg ttcgaggcgc cggccgccga gctgctgctc accgcacacc
     3661 gcgagctcga gaagctgacg ctgactgcgc gccagcagcg cgtgaaggag ctgctggcgc
     3721 tgccctacgg cgacctggtg cacgaagggc agctgctcga tccggtgtgc cgcgacatcg
     3781 aggcgctgct cagctcctcg cagcaacgcg tcaccggcga ggtgcggctg ctgctgcgca
     3841 ccggaagcct gttcgtcgag ggggtggagt cgccccactc gctgatggcg gcatcgcgcg
     3901 gcgtatacgg cgaggccgcg ggcgagtggt cggcgcgcga tgcgctcggc ttctcgcgca
     3961 tcgtcgcgct cccgggcgtg tttcatgccc gcgccgcggc gcacggcggc ggcacaccgc
     4021 ccggggagcg ctgaccgtgg tcagcatgcg caacgtggtg gtggacaagc tcgcctcggt
     4081 gacgcaggcc tgcggcctgt cgcacgaggt gcgcatctcc gccgatatcc ccgccgagga
     4141 aggcgtggtg atcgtggtcg aggtgctcaa caacaagtcg acctacaaca ccctcgagct
     4201 cgtcagcggc cgcatggcca aggtcggcaa gggcgacatc gtggcggggg cgctcgggca
     4261 ccgccaggcg ctgttcggct actccggtca cgtgccgccc gcgctgcagc cgggcgacgt
     4321 catccagatg ctcaatatcg gcggcgtgct cggggtgtgc gactcgatca acccggagaa
     4381 gggccggccg ttcgactgcc gggtgctcgg cgtggcgctc aacttcccct acctcggcga
     4441 gcgcatcggc gtcccggccc gtgtcggcca caagccgctc gatgccgcgg cgccgctcga
     4501 ggtgcgcggt gtaccggtgg tggcgctcgc cggcacctgc atggaagccg gcaagacggc
     4561 ggccgcgtgc gcggtgatcg cccgcatgcg ccaccgcggg ctcacggtgg acgccttcaa
     4621 ggcgaccggc gtgtcgctgc ggcgcgatat cctggcgatg gaggatgccg gcgcgcgccg
     4681 ctgctcgatc ttcaccgatc tcggagtggt cagcaccacg cgcgccaacg gtccggcgct
     4741 cacccgcgcc atgctgtccg agctcgcggc cggcaagccc gacgtgctgg tgttcgagct
     4801 cggcgacggc atgctcggca cctacggcgt ggatgccatc ctcgaatgtc ccgacatccg
     4861 cgccgcgctc accgccgtca ttctctcggc c
//
LOCUS       DATDMP010000486         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1412354, whole genome shotgun sequence.
ACCESSION   DATDMP010000486 DATDMP010000000
VERSION     DATDMP010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412354"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VI195_08535"
     CDS             <1..678
                     /locus_tag="VI195_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY6824473.1"
                     /translation="EDPIEMIEPSFNQMQVQHSIGVDFASGIRTLMRQDPDIIMVGEI
                     RDLETAEMAIQAALTGHLVLSTLHTNDSPSAITRMLDLGVPSYLLQSSILGVMAQRLL
                     RTLCPHCKEQRDLEDSAWEHLVAPWKAPKPRGTTYVAKGCLECRMTGFKGRVGIYETM
                     MMTDELKRTITAETDLAVLQEKAYKEGMKPLRISGAMKVAAGLTSVEEVLQTAPPPTG
                     DRRQQSR"
     gene            770..1195
                     /locus_tag="VI195_08540"
     CDS             770..1195
                     /locus_tag="VI195_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6824474.1"
                     /translation="MKDTLKPGLKHRFTFKIPPSKTVPQLYPESASFREMPEVLATGY
                     LVGLLEWACIDAIQPHLDWPREQSLGTHVNLSHLAATPPGLTVTVEVRLDRVEGRKLT
                     FSLSAHDGVDKITEGVHERFVIDAAKFRSKVAEKARRVS"
     gene            1213..1614
                     /locus_tag="VI195_08545"
     CDS             1213..1614
                     /locus_tag="VI195_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY6824475.1"
                     /translation="MLAAAALLASCAQEPAGPSAPLQDTFWRTVEIEGVPYAPRPGVR
                     EPYLLFARDRVTGFTGCNNLTGGYEQDAARLRFKPVAMTRMACLPDNDLETRFVSAIN
                     ATTSQRITGSRLELLDGTGRTRMRLEARDPR"
     gene            1620..>2074
                     /locus_tag="VI195_08550"
     CDS             1620..>2074
                     /locus_tag="VI195_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY6824476.1"
                     /translation="MAQAAYVPKAQEARNKAVVERYFREVLDQKKFDVMPDLMAADAV
                     LHRPGLDVNGLPAAIQRLRAVLQDFTDFSTEISGLIAEGDMVAVRVRHRTRVRPHAFR
                     SRAGEVMTGEQALEWTAIVQFRLKDGKIAEEWVMRDELGMLLQLGRVSVA"
BASE COUNT          377 a          701 c          659 g          337 t
ORIGIN      
        1 gaggacccga tcgagatgat cgagccctcc ttcaaccaga tgcaggtgca gcattcgatc
       61 ggcgtggact tcgcgagcgg catccgcacc ctgatgcggc aggatccgga catcatcatg
      121 gtgggcgaga tccgcgatct cgagaccgcc gagatggcga tccaggcggc gctgaccggc
      181 cacctggtgc tctcgaccct tcacaccaac gattctccgt cggccattac ccgcatgctc
      241 gacctgggcg tgccgtccta cctgctgcag tcctccatcc tcggcgtcat ggcgcagcgg
      301 ctgctgcgca cgctctgccc tcactgcaag gagcagcgcg acctcgagga ctcggcctgg
      361 gagcatctcg tcgcgccgtg gaaggctccc aagcccaggg gcaccacgta tgtcgccaag
      421 ggttgcctgg agtgccgcat gacaggcttt aaaggtcgag tcggcatcta cgaaacgatg
      481 atgatgacgg acgagctgaa gcgtacgatt accgccgaga ccgacctggc tgtgttgcag
      541 gagaaagcct acaaggaagg catgaagccg ctgcgcatca gcggcgcgat gaaagtcgca
      601 gccggcctga cctcagtcga agaagtgcta cagacggcgc caccgcctac cggtgaccgg
      661 cgccagcagt cgcgctagcg ccgcaactta tgcggcgcgg caatgagtgg ggggtaatgg
      721 gccataatgc ctgaacccga tttgccacat tactcatcac ccatcaccga tgaaggacac
      781 gctcaagccc ggccttaagc atcgcttcac tttcaagatt ccgccgtcca agaccgtccc
      841 gcagctctat cccgagtccg cgtccttccg ggagatgccg gaagtgctgg cgaccggcta
      901 cctggtcggg ctgctggaat gggcctgcat cgacgcgata cagccacacc tcgattggcc
      961 ccgcgagcag tccctcggca cccatgtcaa cctctcgcac ctcgcggcga cgccgcccgg
     1021 gctcaccgtg acggtcgagg tccgcctcga ccgcgtcgaa ggccgcaagc tcaccttctc
     1081 cctttcagcc catgacggcg tggacaagat caccgagggc gtccacgagc gttttgtcat
     1141 cgacgccgcg aaattcaggt cgaaagtcgc ggagaaagcg aggcgcgtgt cgtgacccgg
     1201 aatccgacct ggatgctggc cgcagcggca ctgctcgcga gttgcgcgca ggagccggcg
     1261 gggccgtccg ccccgctgca ggatacgttc tggcggacag tggagatcga aggcgtgccg
     1321 tatgcgcccc gcccgggcgt ccgcgagcca tacctcctgt ttgcccggga tcgcgtgacc
     1381 ggcttcaccg gctgcaacaa cctgaccggc ggctacgagc aggacgctgc ccgcctgcgc
     1441 ttcaagcccg tggcgatgac gcggatggcc tgcctcccgg ataacgatct cgagacgcgc
     1501 ttcgtgtccg ctatcaacgc caccacttcg cagcgcatca cgggctcccg cctcgagctg
     1561 ctcgacggca cgggcaggac aagaatgcgg ctcgaggcgc gagacccgag gtagccgccg
     1621 tggcccaggc cgcctacgtt cccaaggccc aggaagcgcg caacaaggcc gtggtcgagc
     1681 gctatttccg cgaggtgctc gaccagaaga agttcgacgt gatgccggat ctgatggcgg
     1741 cggacgcggt cctgcaccgc ccgggcctcg acgtgaacgg actccccgct gccattcagc
     1801 gcctgcgcgc ggtgctccag gacttcaccg atttttccac ggagatttcc ggcctgatcg
     1861 ccgaaggcga catggtcgcg gttcgcgtcc gccatcgcac gcgggtgcgg ccgcacgctt
     1921 tccgcagccg cgccggcgaa gtgatgaccg gcgagcaagc gctggagtgg acggcgatcg
     1981 tccaattccg cctgaaggac ggcaagatcg ccgaagaatg ggtgatgcgc gacgagctcg
     2041 gcatgcttct ccagcttggc cgggtgtcgg tggc
//
LOCUS       DATDMP010000487         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2078608, whole genome shotgun sequence.
ACCESSION   DATDMP010000487 DATDMP010000000
VERSION     DATDMP010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078608"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VI195_08555"
     CDS             <1..661
                     /locus_tag="VI195_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824477.1"
                     /translation="DIAQDLYSSGAEVALVQRSPTLIVNIEPSAQLPYALYDEGPPLE
                     DCDLLTAGMPLPLARRTHQILTRRAKDMDRALLERLERVGFRMDFGEDGTGWQFKYLT
                     RGGGYYFNVGCSELIVEGKIGFAPISDLNKFLEKADLVVLATGYKGQEHLVKKLFGEA
                     VAARVGEIWGFGEDQELRNMFRRTAQPGLWFIAGSFAQARIYSKVLALQIKADELGLA
                     S"
     gene            658..975
                     /locus_tag="VI195_08560"
     CDS             658..975
                     /locus_tag="VI195_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6824478.1"
                     /translation="MNEAYSDKEPTRAEVDALSGPTLVEFGSPGCGYCRRTQPLLAEA
                     LREHAGVRHLKIADGSGRRLGRSFAVKLWPTLVFLRDGKEVSRLVRPAGADEIRNALE
                     AIQ"
     gene            972..1232
                     /locus_tag="VI195_08565"
     CDS             972..1232
                     /locus_tag="VI195_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824479.1"
                     /translation="MNPEKIKVRTETGQVLEVVVLNKRAEAIEVVIGEGVHNMRCELK
                     PTRNGLAYAGSIRGREIVYERSQAQVEADLKRDAPGRRDYSR"
     gene            1282..2238
                     /locus_tag="VI195_08570"
     CDS             1282..2238
                     /locus_tag="VI195_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6824480.1"
                     /translation="MGALNRWLSLRGYGLVTSALFGATTSPLKMRARFERLSAVSRER
                     LKRKYPKVAFADHAAGQLAIESVRATESPARAILYLHGGAFFMGSSASYRNRAMRLSY
                     RCNAEVFVPNYRLAPERPYPAAFDDALAAWDSIRALRPGAALFVAGDSAGGGLGLSLL
                     VRLRDLGMKMPDGALLLSPWTDLTVSGTSVDGNRGKDLWFTRRHLEAWAAYYTAGADP
                     RSPYVSPVLADMSGLPPLLLFVGENELLLDDALRVRDAATSAGTEVRLHVGKGMQHDW
                     PLTLPWLEESQAAWSAMRNFVEEHDARCLSYAINEGWPKSTQ"
     gene            2217..>2474
                     /locus_tag="VI195_08575"
     CDS             2217..>2474
                     /locus_tag="VI195_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824481.1"
                     /translation="MAKEHAIKAAQEELPRLGARGAVLDVQRRGHDAIIVLDDYSTIT
                     VSGLLAVKLIKLLTSRYLRNWSLRSSASAGSPLRGKVQVDSA"
BASE COUNT          433 a          845 c          785 g          411 t
ORIGIN      
        1 cgacatcgcc caggacctct actcgagcgg cgccgaggtc gcgctggtgc agcgcagccc
       61 gacgctgatc gtcaacatcg agccgagcgc gcagcttccc tacgcgctct acgacgaagg
      121 gccgccgctc gaggactgcg acctcctcac tgccggcatg ccgctgccgc tcgcgcggcg
      181 cacgcaccag atcctcaccc ggcgagcgaa ggacatggac cgcgcgctcc tcgagcgcct
      241 cgagcgcgtc ggcttccgca tggacttcgg cgaggacggc accggctggc agttcaagta
      301 cctcacgcgc ggcggcggct actacttcaa cgtcggctgc tcggagctga tcgtcgaagg
      361 caagatcggc ttcgcgccaa tttccgatct gaacaaattt ctggaaaaag cagatctggt
      421 ggtgctcgct accggctaca agggccagga gcacctggtg aagaagctct tcggcgaggc
      481 ggtcgcggcc agggtcggcg agatctgggg cttcggcgag gaccaggagc tgcgcaacat
      541 gttccgccgc accgcccagc cgggcctgtg gttcatcgcc ggcagcttcg cgcaggcgcg
      601 catctactcc aaggtgctcg cgctgcagat caaggcggac gagctcggcc tcgcttcatg
      661 aacgaggcct attccgacaa ggagcccacg cgggctgaag ttgatgccct ctccggcccc
      721 acgctcgtgg agttcggcag cccgggctgc ggctactgcc ggcgcaccca gccgcttctc
      781 gccgaagccc tgcgcgagca tgccggcgtg cgccacctca agatcgccga cggcagcggc
      841 cggcgcctcg gccgctcctt cgccgtgaag ctctggccta cgctagtctt cctgcgcgat
      901 ggcaaggaag tttcgcgcct cgtgcgcccg gcgggcgccg acgaaatccg caatgccctg
      961 gaggcgatcc aatgaatccc gagaagatca aggtgcgcac cgagaccggc caggtgctcg
     1021 aggtcgtggt gctcaacaag cgcgccgagg ccatcgaggt cgtcatcggc gagggcgtgc
     1081 acaacatgcg ctgcgagctc aagcccacgc ggaacggcct cgcctacgcg ggcagcatcc
     1141 gcgggcgcga gattgtctac gagcgcagcc aggcgcaggt ggaagcggac ctcaagcgcg
     1201 acgcgcccgg aaggcgcgac tacagccgct gaatcggggc caggcacagc aggccctcct
     1261 acaaccacgg ttagaaggcc gatgggtgca ctgaaccgct ggctctccct gcgcggctac
     1321 ggcctcgtca ccagcgcctt gttcggcgcc accacctcgc ccctgaagat gcgtgctcgc
     1381 ttcgagcgcc tgagcgccgt ctcacgcgag cggctaaagc gaaagtatcc caaggttgca
     1441 ttcgcggacc acgctgccgg tcaactcgca atcgaatccg tccgcgccac cgaatcgcct
     1501 gcgcgggcca tcctgtacct gcacggcgga gcgtttttta tgggctcttc cgcctcttac
     1561 cgaaaccgcg ccatgcgcct gagctaccgc tgcaatgccg aggtttttgt cccgaactat
     1621 cggctcgcgc cggagcgccc ctatccggcc gccttcgacg atgcgctcgc cgcatgggac
     1681 tccatcaggg ccctgcggcc aggcgcagcg ctgtttgtcg ccggcgactc ggcgggcggc
     1741 gggctgggcc tcagtttgct cgtcaggctc cgggatctcg gcatgaagat gccggacggg
     1801 gccctcctgc tctccccgtg gacggacctg accgtttccg gcacctccgt ggacgggaat
     1861 cgcggcaagg acctgtggtt cacccgcagg cacctcgaag catgggccgc ttactacacc
     1921 gctggagcgg atcctcgctc cccgtacgtc tcgcccgtcc tcgccgacat gtccgggctg
     1981 ccaccactcc tgctcttcgt cggagagaac gagctcttgc tcgacgacgc cttgcgtgtt
     2041 cgcgacgccg ccacttcggc cggaaccgag gtccgcctgc atgtcggaaa gggcatgcag
     2101 catgattggc cacttaccct gccatggctc gaggaaagcc aggcagcgtg gagcgcgatg
     2161 agaaatttcg tcgaagagca tgatgcgcgc tgcctgtcgt acgctataaa tgagggatgg
     2221 ccaaagagca cgcaataaaa gcggcccagg aagaacttcc gcgattgggt gcgcgaggtg
     2281 ccgtgctcga cgtgcaacgg cgcggccacg atgcaatcat cgtgttggac gactacagca
     2341 cgatcacggt aagcggtttg cttgccgtga agttgatcaa gctgctcacc agccgctacc
     2401 taagaaactg gtctcttcgc tcgagtgctt cggcgggaag tcctctacgc ggaaaagtgc
     2461 aagtcgattc cgct
//
LOCUS       DATDMP010000488         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_301350, whole genome shotgun sequence.
ACCESSION   DATDMP010000488 DATDMP010000000
VERSION     DATDMP010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301350"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VI195_08580"
     CDS             <1..291
                     /locus_tag="VI195_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY6824482.1"
                     /translation="YLAAAGRLTQAGLRLTALRLDARGAWEFDLADGVTVRLGRRQVD
                     ERFEKFMNAALKLVTQRGEEISYVDMRYTNGFAIGWRSGTRRASGKGGGRDA"
     gene            284..1519
                     /gene="ftsA"
                     /locus_tag="VI195_08585"
     CDS             284..1519
                     /gene="ftsA"
                     /locus_tag="VI195_08585"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY6824483.1"
                     /translation="MLKRSDRNLIVGLDIGTSKVVALVGEVGADGSIELLGLGSQPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCEIHSVFAGIAGSHVRSLNSHGVVGVRDK
                     EVSHGDVEHVIDAAKAVAIPADQKILHVLPQEFIVDGQEGIRDPVGMSGVRLEAKVHI
                     VTGADSAAQNIEKCIQRCGLEVEDVVLEQLASSFAVLTEDEKELGVCLVDIGGGTTDL
                     AVFSNGAIRHTAVIPIAGDQVTNDIAVSMRTPTQYAEDIKIRYACALSQLANPDESIE
                     VPSVGERPARRLARQTLAEVVEPRYEELFALVRDELRRSGFEEVIAAGVVLTGGSSKM
                     EGAIELAEEVFHVPVRLGSPQAVQGLSDVVRNPIFSTGVGLLLYARDNVMPARRGHSL
                     TGNAKSVFDRMRSWFQGNF"
     gene            1583..>2264
                     /gene="ftsZ"
                     /locus_tag="VI195_08590"
     CDS             1583..>2264
                     /gene="ftsZ"
                     /locus_tag="VI195_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY6824484.1"
                     /translation="MFELMDAYSQSAVIKVIGVGGGGGNAVAHMVSAGIDGVEFMCIN
                     TDAQALKHAKVKTALQIGCNITKGLGAGADPEVGRQAAMEDRDRIVELVEGCDMLFIT
                     CGMGGGTGTGAAPVVAQVAKELGILTVAVVTKPFEMEGNKRSFVADHGIAELGKYCDS
                     LITIPNQKLLTVLGPQTTLLDAFKSANQVLQGAVQGIAELITRPGLINVDFADVRTVM
                     AETGMAMMG"
BASE COUNT          396 a          729 c          792 g          347 t
ORIGIN      
        1 tatctcgccg ccgccggacg gctgacccag gcaggtctgc gcctgacggc gctgcggctc
       61 gacgcgcgcg gcgcctggga attcgatctc gccgacggcg tcacggtgcg cctcggcaga
      121 cggcaggtcg acgagcgctt cgagaaattc atgaacgccg cgctgaagct cgtcacgcag
      181 cgcggcgagg aaatctccta cgtggacatg cggtatacga acggatttgc catcggctgg
      241 cgcagcggca cgcggcgcgc gagcggcaaa ggcgggggcc gcgatgctta agcgctcgga
      301 ccgtaacctg atcgtcggcc tcgacatcgg cacctccaag gtggtggcac tggtcggcga
      361 agtaggcgcc gacggctcga tcgagctgct cgggctcgga tcgcagccct cgcgcggcct
      421 gaagaaggga gtcgtggtca acatcgagtc cacggtgcaa tccatccagc gcgccgtgga
      481 ggaggccgag ctcatggccg gctgcgagat tcactcggtg ttcgccggca tcgccggcag
      541 ccacgtgcgc agcctcaact cccacggcgt ggtcggggtg cgcgacaagg aggtgtcgca
      601 cggcgacgtc gagcacgtga tcgacgccgc caaggcggtg gccattcccg ccgatcagaa
      661 gatcctgcac gtgctgccgc aggagttcat cgtcgacggt caggagggca tccgcgatcc
      721 ggtgggcatg tccggggtgc ggctcgaggc gaaggtgcac atcgtcaccg gggccgactc
      781 cgcggcgcag aacatcgaga agtgcatcca gcgctgcggc ctggaggtcg aggacgtggt
      841 gctcgagcag ctggcatcga gctttgcggt cctcaccgag gacgagaagg agctcggcgt
      901 gtgcctggtc gacatcggcg gcggcaccac cgatctggcg gtgttctcca acggcgcgat
      961 ccgccacacc gcggtgatcc cgatcgccgg cgaccaggtc accaacgaca tcgcggtctc
     1021 gatgcgcacc ccgacgcagt acgccgagga catcaagatc cgctacgcct gcgcgctctc
     1081 gcagctcgcc aaccccgacg agagcatcga ggtaccgagc gtgggcgagc gcccggcgcg
     1141 gcgcctcgcg cgccagacgc tcgccgaagt cgtcgagccg cgctacgagg agctgttcgc
     1201 gctggtgcgc gacgagctgc gccgcagcgg cttcgaggag gtgatcgccg ccggcgtcgt
     1261 gctcaccggg ggcagctcca agatggaagg cgccatcgag ctcgccgagg aggtgtttca
     1321 cgtgccggtg cgcttaggca gcccgcaggc ggtgcagggc ctcagcgacg tggtgcgcaa
     1381 cccgatcttt tccacgggcg tgggcctgct gctctacgcg cgtgacaacg tcatgcccgc
     1441 gcggcgcggg cactccctca ccggcaacgc caagtcggtc ttcgaccgca tgcgttcctg
     1501 gttccagggc aacttttgaa gctgacagag cgaatgacga ccgactcaac caaccgtgtt
     1561 tttcgtgtaa ggagcctgac acatgtttga attgatggac gcatacagcc agagcgccgt
     1621 gatcaaagtg atcggcgtgg gcggcggcgg cggcaacgcc gtggcgcaca tggtcagcgc
     1681 cggcatcgac ggcgtggagt tcatgtgcat caacaccgat gcgcaggcct tgaagcatgc
     1741 caaggtgaag acggcactgc agatcggctg caacatcacc aaggggctcg gcgccggcgc
     1801 cgacccggag gtcggccggc aggcggcgat ggaggaccgc gaccgcatcg tcgagctcgt
     1861 cgagggctgc gacatgttgt tcatcacctg cggtatgggc ggtggaacgg gcaccggagc
     1921 ggcgccggtg gtcgcgcagg tcgccaagga gctgggcatt ctgaccgtcg ccgtcgtgac
     1981 caagcccttc gagatggaag gcaacaagcg ctcctttgtc gccgatcacg gcatcgccga
     2041 gctgggcaag tactgcgact cgctcatcac tatcccgaat cagaagctgc tgaccgtgct
     2101 cgggccgcag accacgctgc tggatgcgtt caagtcggcc aaccaggtac tgcagggtgc
     2161 cgtgcagggc atcgcggagc tgatcacgcg ccccggcctg atcaacgtcg acttcgccga
     2221 cgtgcgcacc gtcatggcgg aaaccggcat ggcgatgatg ggct
//
LOCUS       DATDMP010000489         2721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_634288, whole genome shotgun sequence.
ACCESSION   DATDMP010000489 DATDMP010000000
VERSION     DATDMP010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634288"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VI195_08595"
     CDS             complement(<1..306)
                     /locus_tag="VI195_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY6824485.1"
                     /translation="MSAARRNLPQRPAEERRIVPERRDRMRRINIIHFVGIGGSGMSG
                     IAAVLLNLGYRVQGSDARESAVTQWLAGLGARITIGHAADNVAGADVVVVSGAVPRDN
                     "
     gene            complement(303..1388)
                     /gene="murG"
                     /locus_tag="VI195_08600"
     CDS             complement(303..1388)
                     /gene="murG"
                     /locus_tag="VI195_08600"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY6824486.1"
                     /translation="MSRPVLIMAGGTGGHVFPALALARLLRAQALEVVWLGTERGLES
                     RIIPAEGIPIEHLSVGGLRGKGTLSWLAAPFRLTRALLQALAIMRRYRPLVVVGLGGF
                     VTGPGGLAAWLARRPLVIHEQNAIAGLTNRVLAHLARQVLEAFPGSFGKGVHARAIGN
                     PVRADISALPPPATRFAGRSGPMRLLVIGGSQGATRLNAVVPFALKRLAGTLAFDVRH
                     QAGERWLEAGRTSYAEAGVRAEVRPFIEDMSEAYGWADLVICRAGALTVSELAVAGVA
                     AILVPFPHAVDDHQTSNAQYLVRESAAVLIPDRELTAERLASELRRVCVGRGRLLAMA
                     ERARLLAKPRAADELAASCLALARSAA"
     gene            complement(1385..2569)
                     /gene="ftsW"
                     /locus_tag="VI195_08605"
     CDS             complement(1385..2569)
                     /gene="ftsW"
                     /locus_tag="VI195_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY6824487.1"
                     /translation="MSGAALSGFDRSGARGNAVHLDAVTLALIAAIVLLGLIMVTSAS
                     VSIASQDSGQAFYYLERQLLLTLIGLACAALLFALPSARLEKAAVPLLIAAFVLLLLV
                     LVPGLGHAVNGSRRWLRLSGANFQVSELARVLVLIYLASYAVRHEAQLRESLAGLAKP
                     LALLCCVSALLLAEPDFGAATVLFATGFGLLFLAGARLRYVIAMTAIAASGFAVIAVS
                     SSYRVRRLTAFLNPWADPFNSGFQLTQSLIAIGRGQWFGVGLGDSVQKLFYLPEAHTD
                     FLFAVLAEELGLVGVVLTLALFLALIWRSFHIARLAADAGLKFPAYLAAAFGLWVGIQ
                     AFINIGVNMGVLPTKGLTLPLMSYGRSSLIVALAWTGILLRVYHEALREQRGSATVRG
                     GQ"
BASE COUNT          482 a          958 c          946 g          335 t
ORIGIN      
        1 gttgtcacgc ggcacggctc ccgagaccac caccacatcc gcgccggcga cgttgtccgc
       61 cgcgtgaccg atggtgatgc gcgcgccgag acccgcgagc cattgggtga cggcgctctc
      121 gcgggcgtcc gagccctgca cgcggtagcc gagattgagc agcacggcgg cgatgccgct
      181 catgccgctg ccgccgatac cgacgaagtg gatgatattg atgcggcgca tgcgatcgcg
      241 ccgctccggc acgatgcgcc gctcctcggc gggccgctgc ggcaggttgc gccgtgcggc
      301 gctcatgcgg cactccgcgc gagcgcgagg cacgaggcgg cgagctcatc ggcagcccgc
      361 ggcttggcga gcaggcgcgc ccgctcggcc atcgccagga gcctgccgcg accgacgcac
      421 acgcgccgca gctcgctcgc gagacgctcg gccgtgagct cgcggtcggg aatcagcacc
      481 gccgcactct cacgcaccag gtactgtgcg ttgctcgtct ggtggtcatc gaccgcgtgc
      541 ggaaaaggaa cgaggatcgc cgcgaccccc gcgaccgcga gctcggacac ggtgagcgcg
      601 ccggcgcggc agatcaccag atccgcccat ccgtaggcct cgctcatgtc ctcgatgaag
      661 ggacgcacct cggcacgtac cccggcttcc gcatagctcg tgcgtccggc ctcgagccag
      721 cgctcaccgg cctggtggcg cacatcgaag gcgagcgtcc cggcgaggcg cttgagcgcg
      781 aagggcacta ccgcgttcag ccgcgtggca ccctggctgc cgccgatcac cagcagccgc
      841 atgggtcccg agcgcccggc aaagcgtgtc gcgggcggcg gcagcgcgct gatgtccgcg
      901 cgcactggat tgccgatggc gcgcgcgtgc acgcccttcc cgaaacttcc gggaaaagcc
      961 tcgagcacct ggcgggcgag atgcgcgagc acgcggttgg tgaggccggc gatggcattc
     1021 tgctcgtgga tcacgagcgg acggcgcgcg agccaggcgg cgagcccgcc agggccggtg
     1081 acaaatccgc cgaggcccac gaccacgagc ggccggtagc ggcgcatgat ggcgagcgcc
     1141 tgcagcagcg cgcgtgtcag gcggaaaggc gccgccagcc agctgagggt gcccttgcca
     1201 cgcagcccgc cgaccgacag gtgctcgatc ggaatacctt cggcggggat gatgcgcgac
     1261 tcgagtccgc gctcggtgcc gagccagacc acttccagcg cctgcgcgcg cagcagtcgc
     1321 gcgagcgcga gtgcgggaaa cacgtgtccc ccggtgccgc ccgccatgat gagtactgga
     1381 cggctcactg gccaccccgc accgtggcgg agccgcgttg ctcacgcagc gcctcgtggt
     1441 acacacgcag taggatcccg gtccaggcga gcgcgacgat caggctcgag cgtccatagc
     1501 tcatcagcgg cagcgtcagt cccttggtcg gcagcacccc catgttcacg ccgatattga
     1561 tgaacgcctg gatgccgacc cataggccga aggcggcggc gagatacgcg ggaaatttca
     1621 acccggcgtc ggcggcgagg cgcgcgatgt gaaagctgcg ccaaatgagc gcgagaaaaa
     1681 gcgcgagcgt gagcacgacg ccaacgagcc cgagctcctc cgccagcacg gcgaacagga
     1741 aatccgtgtg cgcctccggc aggtaaaaca gcttctgcac cgagtcgccg agaccgacgc
     1801 cgaaccactg cccacggccg atcgcgatca gcgactgggt gagctggaag ccgctgttga
     1861 aaggatccgc ccaggggttg aggaaggcgg tcaaccgccg cacgcggtag ctggagctga
     1921 ccgcaatcac cgcaaaccca ctggctgcga tcgcggtcat ggcgatcacg taacgcagcc
     1981 gcgcgccggc gaggaacagc aggccgaagc ccgtggcgaa cagcaccgtc gcggcgccga
     2041 agtccggctc ggcgagcagc aatgcgctca cgcagcacag cagcgccaac ggcttcgcga
     2101 gcccggcgag cgattcgcgc agctgcgcct cgtgacgcac ggcgtagctc gccaggtaga
     2161 tgagcaccag cacccgcgcc agctctgaca cctggaaatt ggctcccgaa agccgcagcc
     2221 agcgccggct gccattcacc gcatggccga gaccggggac gagcacgagc agcagcagca
     2281 cgaaggccgc gatgagcagc ggaaccgccg ccttctcgag ccgcgcgctc ggcagcgcga
     2341 acagcagtgc cgcgcaggcg agtccgatga gcgtcagcag cagttgccgc tcgaggtagt
     2401 agaaggcctg gcccgagtcc tggctggcga tcgacaccga agcggaggtc accatgatca
     2461 gcccgagcag cacgatcgcg gcgatgagtg cgagtgtgac tgcatcgagg tgcacggcgt
     2521 tgccacgggc accgctgcga tcgaatccac tgagcgcggc gccgctcatg cggcaagctc
     2581 ctgcaccgct tcgacgaaca ccgcggcgcg gtgcgcgtag tcctcgaaca tgtcgaagct
     2641 cgcgcacgcc ggcgagagca gcaccgtgtc gccggcgcgc gccacgcggg cggcggcctg
     2701 cactgcctga ggaagcgtca c
//
LOCUS       DATDMP010000490         3675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_301622, whole genome shotgun sequence.
ACCESSION   DATDMP010000490 DATDMP010000000
VERSION     DATDMP010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301622"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VI195_08610"
     CDS             complement(<1..607)
                     /locus_tag="VI195_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEY6824488.1"
                     /translation="MPSASGNQQGEASAAPGGRAQPVLIYDGDCTFCVYWARYWQRLT
                     GERVVYRPYQEAANEYPAIPLADFQRAVQFIAPDGRRASAAEASFLTLSHAPHRGLWL
                     WLYRRLPGFAFLAERAYAFTAAHRPAFFRISRWLWGSQLEPARYQLVSFLFLRGFGLI
                     YLSAFLSFAVQAQGLIGSHGLLPLTELTDAVAASSGSERFWL"
     gene            795..1544
                     /gene="phbB"
                     /locus_tag="VI195_08615"
     CDS             795..1544
                     /gene="phbB"
                     /locus_tag="VI195_08615"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY6824489.1"
                     /translation="MGASQRVALVTGGMGGLGEAICIKLAAFGYTVVTTCSPGNSRAE
                     QWLREMRERGHPFHAYPCDVTDWDSCVACVRRIVSEVGPVDVLINNAGITRDMTFRKM
                     DRASWDAVMHADLDSCFNMTKQVCDGMVERGWGRIINISSVNGQKGAFGQTNYAAAKA
                     GMHGFTKSLALELARKGVTVNTISPGYIGTKMVMAIPREVLDSKIIPQIPMGRLGKPE
                     EVAGLVAYLSSEEAAFVTGANIAINGGQHMQ"
     gene            1656..2126
                     /gene="msrB"
                     /locus_tag="VI195_08620"
     CDS             1656..2126
                     /gene="msrB"
                     /locus_tag="VI195_08620"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY6824490.1"
                     /translation="MSEFDLRPPSEAQRTQIIEGLNRDERRVLLEHGTEAAFCGVFLD
                     NHRDGIYTCRFCGLPLFRSSAKFDSGTGWPSFFRPYDESHIRRVRDTSYGMIRTEEVC
                     ARCGSHLGHVFPDGPPPTGERHCLNSVSLSFTDERQALPDILGRGAPEGKPDRA"
     gene            2147..3040
                     /locus_tag="VI195_08625"
     CDS             2147..3040
                     /locus_tag="VI195_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY6824491.1"
                     /translation="MSILLIDQRRRSLGRFEVGRVLPHPDRHMVGPFIFFDHLGPVEL
                     PQGLPREADVRPHPHIGLSTVTYVFAGEIMHRDSTGIEQAIRPGEVNWMTAGRGITHS
                     ERFERARREGGPMHAIQSWVALPDREEETAPLFAHHAAADLPVQQERGLWLRLLAGTA
                     YGLRSAVKTHSPLFYLHITLEPSARIELPGEYPERAAYVVSGSVRAAGQSVEPGHMIV
                     FERNAPAVIEALAPSVLMLLGGEPVGPRFIEWNFVSSSRERIEQAKADWRAGRLKLPD
                     ADNAEFIPLPEDAHPSANAMS"
     gene            3054..3440
                     /locus_tag="VI195_08630"
     CDS             3054..3440
                     /locus_tag="VI195_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY6824492.1"
                     /translation="MAHASADIGTINYTVSLVAGEHTFTADERAAAGGQDAGPSPHEM
                     LCAALGACTAITLRMYAQRKEWTLRAVHVDVRLQIEGKEHRITRRLRLEGDLDDAQRA
                     RLADVAERTPVTLTLKQGAIITTILE"
     gene            3471..>3675
                     /locus_tag="VI195_08635"
     CDS             3471..>3675
                     /locus_tag="VI195_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824493.1"
                     /translation="MILARPLPALCVLVLASSAMLAACSTMRLQAPDIIPTSVELVDA
                     QVTGQRFKVGMHVQNPNDRALPIK"
BASE COUNT          654 a         1202 c         1221 g          598 t
ORIGIN      
        1 tgagccagaa gcgctcgctg ccgctgctgg cggcgacggc gtcggtcagc tccgtgaggg
       61 gcaggaggcc gtggctgccg atgagcccct gcgcctgcac ggcgaaggaa agaaaggcgg
      121 agaggtagat cagtccgaac ccgcgcagga acaggaacga gaccagctgg taccgcgcag
      181 gctcgagctg actgccccag agccagcgac tgatgcggaa gaaggccggg cggtgggccg
      241 cggtaaaggc gtaagcgcgc tcggccagaa aggcaaatcc gggcagcctg cgatagagcc
      301 acagccacag gccccggtgg ggcgcgtgac tgagagtcag gaagctcgcc tcggcggcgc
      361 tcgcgcggcg accgtcgggg gcgatgaact gcacggcacg ctgaaaatca gcgagcggta
      421 tcgccgggta ctcgtttgcc gcctcctggt atgggcggta cacaacccgc tcacccgtca
      481 gacgctgcca gtaccgggcc cagtacacgc agaacgtgca atcgccatcg tagatcagca
      541 ccggctgggc cctaccgccg ggcgcagcgg acgcttcgcc ctgctgattg ccggatgccg
      601 aaggcatggc gccactctaa attaatcaca ggggcgccgc atgtgcccgg caagcggctg
      661 gccgcgggcg catacccagg cgtccacctt gtaggtacat ctcacatgag cagtgccgca
      721 ggtgcggatg ccaggcgcat cgggggcagg cagaatgatc gtcggcaact gcgccagtct
      781 gtgagggtcc atcgatgggt gcgagtcagc gcgtggcact ggtcactggc ggaatgggtg
      841 ggctcggtga agcgatctgc atcaagctcg cagcgttcgg ctatacggtc gtcaccacct
      901 gctcgccggg caattcccgc gccgagcaat ggctgcgaga gatgcgcgag cgcgggcatc
      961 ccttccatgc ctatccctgc gatgtgactg actgggacag ttgcgtggcg tgcgtccgca
     1021 ggatcgtgag cgaagtcgga cccgtcgatg tgctgatcaa caacgccggc atcacgcgcg
     1081 acatgacgtt ccgcaagatg gaccgggcca gctgggatgc ggtcatgcac gccgacctcg
     1141 actcatgctt caacatgacc aaacaggtat gcgacggcat ggtcgagcgg ggctggggcc
     1201 gcatcatcaa tatttcctcg gtcaacggcc agaagggcgc cttcggtcag accaattacg
     1261 cggcggccaa ggccggtatg catggcttca cgaaatcact ggcgctcgag ctggcacgca
     1321 agggcgtcac ggtcaacacc atctctcccg ggtacatcgg caccaagatg gtcatggcga
     1381 tcccgcgcga ggtgctcgac agcaagatca ttccgcagat tcccatgggt cgcctcggta
     1441 agcccgagga ggtggcgggc ctcgtcgcct atctctcgag cgaggaagcg gccttcgtga
     1501 ccggcgccaa tatcgccatc aacggcggac agcacatgca gtgagcgcgc ggctaccgcc
     1561 gcgccgcgca gatcgagccc gagccacccg cgcgcacacg ttccgctcta caatcggctg
     1621 ccgcggcccg cgcgttgcaa gccccgagga gcgtcatgag cgagttcgac ctgcggcctc
     1681 cgagcgaggc gcaacgtacc cagatcatcg agggactcaa ccgcgacgaa cgccgcgtgc
     1741 tgctcgagca cggcaccgag gccgcctttt gcggcgtgtt cctcgacaat catcgtgacg
     1801 ggatctacac gtgccgcttc tgcgggctac cgctgttccg ctcgagcgcg aagttcgact
     1861 cgggcacggg ctggccgagc ttcttccggc cgtacgacga gtcgcacatc cgccgcgtgc
     1921 gcgataccag ctatgggatg atccgcaccg aggaggtgtg tgcgcgctgc ggcagtcacc
     1981 tgggacacgt attcccggat ggaccgccgc ccaccggcga gcgtcactgt ctcaattccg
     2041 tgtcactctc ctttaccgat gagcgccagg ccttgccgga tatccttggg cgcggtgcgc
     2101 ccgagggtaa gccggaccgc gcctgagctc agcattcaca gcgaacatga gcatcctatt
     2161 gatcgatcag cggcggcgca gcctcgggcg cttcgaggtc ggccgcgtgc tgccgcatcc
     2221 cgaccggcac atggtgggtc ccttcatctt cttcgaccat ctggggcccg tggagctacc
     2281 gcaaggcctg ccgcgcgaag ccgatgtgcg gccgcatccg catatcggcc tctccaccgt
     2341 cacctatgtg tttgcgggcg agatcatgca tcgcgactcg accggcatcg agcaggcaat
     2401 tcgccccggg gaagtgaact ggatgacggc gggccgcggg attacgcact ccgagcgctt
     2461 cgagcgggca cgacgcgagg gcggacccat gcacgcgatc cagtcctggg tcgcacttcc
     2521 cgatcgggag gaggagaccg caccgctctt cgcgcatcac gccgcggccg atcttcctgt
     2581 ccagcaggag cgcggcctgt ggctgcgtct gctcgcgggt acggcctacg gcctgcgctc
     2641 ggcagtgaag actcattcgc cgcttttcta tctccacatc acgctcgagc cgtcggcgcg
     2701 catcgagctg ccaggcgaat acccggagcg cgcggcctat gtcgtctccg gcagtgtgcg
     2761 cgcggctggg cagagcgtcg aaccagggca catgatcgtc ttcgagcgca acgcgcctgc
     2821 ggtgattgag gcgcttgctc cgagcgtgct gatgctgctc ggtggtgagc ctgtcgggcc
     2881 gcgcttcatc gaatggaact tcgtttcctc atcgcgggag cgcatcgagc aggccaaggc
     2941 ggactggcgc gccggcagat tgaaacttcc cgatgcggac aacgcggagt tcattcctct
     3001 gcccgaggat gcgcacccga gcgccaacgc gatgtcctga actgcgagca tttgtggcac
     3061 acgcgagcgc cgatatcggc acaatcaact acacggtgtc gctcgtggcc ggggagcaca
     3121 ccttcacggc cgatgagcgg gctgcggcgg gaggtcagga cgccgggccc tcgccacatg
     3181 aaatgctgtg cgcggcgctc ggcgcctgca cggcgatcac cctgcgtatg tacgcgcaac
     3241 gcaaggagtg gacgctgcgt gccgtgcacg tggacgtgcg gctgcagatc gaaggcaagg
     3301 agcatcggat cacacggcgg ctgcgcctcg agggagatct cgatgacgcg cagcgtgcgc
     3361 gcctcgccga tgtcgccgag cgcacgccgg tcaccttgac gctgaagcaa ggcgccatca
     3421 tcaccaccat tctggagtga gccggcgatt cgcgcgcgag gaggttgccg atgatccttg
     3481 ccaggccgct gccggcactt tgcgtactcg tgctggcgag ctctgccatg ctggccgcct
     3541 gttccaccat gcgcctgcag gctcctgaca tcatccccac atcggtcgag ctcgtcgacg
     3601 cgcaggtcac cggacagcgc ttcaaggtgg gcatgcacgt gcagaatccc aatgatcgcg
     3661 cgctgccgat caagt
//
LOCUS       DATDMP010000491         2581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1301667, whole genome shotgun sequence.
ACCESSION   DATDMP010000491 DATDMP010000000
VERSION     DATDMP010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301667"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1462
                     /locus_tag="VI195_08640"
     CDS             245..1462
                     /locus_tag="VI195_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824494.1"
                     /translation="MNNSTSTGRAVSVAGSTAVGILAAISFCHLLNDMMQSLLPALYP
                     MLKSSYRLSFGHIGLLTFTFQFTASLLQPLVGALADRTPRPYSLAAGMGFTLVGLVLL
                     AFAGSYALLLVAAALIGTGSSVFHPESSRVARMASGGKHGLAQSVFQVGGNLGSSAGP
                     LLAAFIVLPHGQESVAWFSCAALLGIFLLVNVGHWYKAHGVSRLASARSAPRAAPRHH
                     VAAALVVLLMLVFSKYVYLASITSYYTFYLIDHFHVSVRSSQLHLFVFLASAAVGGMI
                     GGPLGDRLGSKYIIWASILGVLPFTLLLPHANLFWTGVLTVPIGLILASAFPAIVVYG
                     QDLLPGRTGTVAGLFFGLAFGMGSVGAAVLGKLADHVGVDVIYQICAFLPLIGLLAAF
                     LPDLHQSATAQEA"
     gene            1464..1946
                     /locus_tag="VI195_08645"
     CDS             1464..1946
                     /locus_tag="VI195_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HEY6824495.1"
                     /translation="MLKLLPQSLLAELAARAAASPRLRAHHNIHQHSADRVQRFIVVA
                     EGDSYFRPHRHATRAELALLLRGRVDVLLFDEGGRVLARHGVGADAGNLAYETSQGVW
                     HTLVPAAGGCAFLEIKEGPYDPASAAQFADWAPPEGDAAVPAFRSWLRSAQPGDLAPR
                     "
     gene            complement(1963..>2581)
                     /locus_tag="VI195_08650"
     CDS             complement(1963..>2581)
                     /locus_tag="VI195_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY6824496.1"
                     /translation="RLIVSLSDCPVAADLENGFGHAPETVAETIRLAAAAGLVGGSIE
                     DATGDARAPLYEFEHAVERVSAAAQEAHGKRFQFVLTARAENFLHGRADLEDTVRRLQ
                     AFERAGADVLFAPGLPDLAAVRTVCSALKKPVNFMVGMRGKSFTVAQLAAAGVRRISL
                     STSLYRAAITGLLAAAREVSEHGTFGYVENLVSGGELGKYLRPPG"
BASE COUNT          382 a          922 c          808 g          469 t
ORIGIN      
        1 gcggtcgtgc tccaactggg tgcatgcgca caagagcgcg ctggcgagcg gaatcgctgc
       61 cgcacgcagt gtccgggcca tcagagcttc tgcgaaaacc tcaacgcgat gtttcgcggc
      121 tgtccatgat gaccttccgc agagcgcctg acaaaccact ctccatgagc tgcggacccc
      181 gagcgatcag ccgtgtctta agaggtatag gcaagggaca tccgatccgc ttgaatgctc
      241 gctcgtgaac aattcaacct ccacgggcag ggcggttagc gtcgccggca gcaccgccgt
      301 cggcatcctc gccgccatca gcttctgtca cctgctgaac gacatgatgc agtcgctgct
      361 gccggcgctg tacccgatgc tcaagagctc ctaccggctg tccttcggcc acatcgggct
      421 gctcaccttc acctttcagt tcaccgcctc gttgctgcag ccgctggtcg gggctcttgc
      481 ggaccggacc ccgcgtccct actcgctcgc cgccggaatg ggcttcacgc tcgtaggcct
      541 ggtactgctc gccttcgccg gcagctacgc cctgctgctg gtggccgccg cgctcatcgg
      601 caccggctct tcggtgttcc acccggagtc ctcgcgcgtg gcccgcatgg cctccggtgg
      661 caaacacggg ctggcgcaat cggtgttcca ggttggcggc aacctcggct catcggccgg
      721 cccgctgctc gcggccttca tcgtgctgcc gcacggccag gagagcgtcg cctggttctc
      781 gtgtgccgcg ctcctcggca tcttcctgct ggtaaacgtc gggcactggt acaaggcgca
      841 cggggtgtcg cggctcgcga gcgccaggag cgcaccgcga gcggcacccc gccatcacgt
      901 ggcagcggcg ctcgtcgtcc tgctgatgct ggtgttctcg aaatacgtct acctcgccag
      961 catcaccagc tactacacct tctatctcat cgatcatttc catgtgtcgg tgcgcagctc
     1021 acagctgcac cttttcgtct ttctggcttc cgccgcggtc ggcggcatga tcggcgggcc
     1081 gctcggtgac cggctcggca gcaaatacat catctgggca tcgatcctcg gtgtcctgcc
     1141 cttcacgctg ctgctgccgc atgcgaatct cttctggacc ggtgtgctca cggtgccgat
     1201 cggactgatt ctcgcctcgg cctttccggc gatcgtggtc tatgggcagg accttctgcc
     1261 cgggcgcacc ggcacggttg cgggactgtt cttcggcctg gcattcggca tgggcagtgt
     1321 cggcgccgcg gtgctcggca agctcgccga tcacgtcggt gtcgatgtca tctaccagat
     1381 ctgcgctttc ctgccactca tcggtctgct cgcagccttc ttgccggacc tgcaccagag
     1441 cgcaactgcg caggaggcct gagatgctga aactactgcc gcagtcgttg ctcgccgagc
     1501 tcgccgccag agccgccgcg agtccgcgct tgcgtgctca ccacaacatt caccagcaca
     1561 gcgccgatcg ggtgcagcgc ttcatcgtag tggccgaggg tgactcctac ttccggccgc
     1621 accggcacgc cacgcgcgcc gagctggcgc tgctgctgcg cggccgcgtc gatgtgctgc
     1681 tcttcgatga gggtggccgg gttctagcac ggcacggtgt cggtgcggat gccggcaacc
     1741 tcgcctacga gacttcgcag ggggtatggc acacgctggt acccgcggca ggcggctgcg
     1801 ccttcctcga aatcaaggaa ggcccgtacg acccggcaag cgccgcgcag tttgccgact
     1861 gggctccgcc cgagggcgat gcggcggtgc ccgcattccg cagctggctg cgcagtgcgc
     1921 agcccggcga tctggcaccg cgctgaacag gcgcagggca actcaaccgg gcggccgcag
     1981 gtacttgccg agctcgcccc cgctcaccaa gttctcgaca tagccgaagg tcccgtgctc
     2041 gctcacttcc cgtgccgccg cgagcagccc ggtgatcgcc gcacggtaga gcgaggtgga
     2101 gaggctgatg cggcgcacgc cggcggcggc aagctgcgcc acggtgaagg acttgccgcg
     2161 catgccgacc atgaagttca ccggtttctt caacgcactg cacacggtgc gcaccgccgc
     2221 gagatccggc agtcccggcg caaacaacac atcggccccg gcacgctcga acgcctgcag
     2281 ccggcgcacc gtatcctcga gatccgcccg cccgtggaga aaattctccg cgcgcgctgt
     2341 gagcacgaac tgaaatcgct taccgtgcgc ctcctgcgcc gccgcggaca cccgctccac
     2401 tgcgtgctcg aactcgtaga ggggagcgcg cgcatccccg gtcgcgtcct cgatcgagcc
     2461 gccgaccagt cccgcggctg cggccagccg gatggtctcg gcgacggtct cgggcgcgtg
     2521 tccgaagcca ttctccaggt ccgcggccac cgggcagtcg ctcagcgaca cgatgaggcg
     2581 c
//
LOCUS       DATDMP010000492         2684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_746311, whole genome shotgun sequence.
ACCESSION   DATDMP010000492 DATDMP010000000
VERSION     DATDMP010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746311"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VI195_08655"
     CDS             complement(<1..250)
                     /locus_tag="VI195_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824497.1"
                     /translation="MPLRVLTLNQISPRGLGRLPADRYEVGSALADPDAILLRSAELN
                     GVAIAASVRAIGRAGVGVNNIPVAAMSRRGVPVFNTPGA"
     gene            complement(258..1340)
                     /gene="serC"
                     /locus_tag="VI195_08660"
     CDS             complement(258..1340)
                     /gene="serC"
                     /locus_tag="VI195_08660"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY6824498.1"
                     /translation="MRVFNFAAGPATLPVEVLQQVRDELLDWQGSGTSVMEVSHRGKA
                     FMAVAQESEALLRELLGVPSHYRVLFLQGGASGQFAAVPLNLARADSTVDYINTGHWS
                     SKALREAQRYTARVNIAADEAASRYTSVPTQQALRLTPDAAYVHYTPNETIGGVEFPY
                     VPDSGSVPLVADMSSTLLSRPLEVAKFGLIYAGAQKNIGPAGLVVVIVRDDLTGHARP
                     GTPAVWDYHAMAQEGSLLNTPATFAWYVAGLVLRWLKAQGGLAAMAAHNRAKAQLLYA
                     TIDSSGFYSNPVAVNCRSWMNVPFTLADARLDEPFLAGALAAGLANLAGHRSVGGMRA
                     SLYNAMPLAGVEALVAFMRDFARRHG"
     gene            complement(1353..>2684)
                     /locus_tag="VI195_08665"
     CDS             complement(1353..>2684)
                     /locus_tag="VI195_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY6824499.1"
                     /translation="ISTRPPGLAADSGLHAGSYRLSEVQAQAILDMRLHRLTALEQDK
                     IIAEYRELLASITDLSDILGRPERLTQVVRAELIEVRDSYGDARRTEINRDHLDLTTE
                     DLIEPQDVVVTLSHAGYAKSQPLTEYQTQRRGGRGKTATAVKDEDFVEKLFVAHTHDT
                     VLCFSNRGKVYWLKVYELPQAGRGARGKPMVNLLPLEEGEKINALLPVKQYDEQHFVF
                     MATRAGTVKKTPLAAFSRPRTTGIIGIDLAPNDQLVGVALTDGEREIMLVSSGGKAIR
                     FHESEVRPMGREAAGVRGIKLGAGQDVIALIVAGEGAGSGTDDAPRVLTASATGYGKL
                     TPLAEFPCHGRGGQGVIALQTSDRNGFTVAALQVIPGQEIMLISSTGTLVRTTVDEIS
                     VQGRNTQGVRLIRLGEGERLVGIERIESLDGDDEEPLVEPAPGATPPAADG"
BASE COUNT          414 a          914 c          919 g          437 t
ORIGIN      
        1 tggctcccgg ggtgttgaac actgggacgc cgcggcggct catcgccgcg accggaatgt
       61 tgttgacgcc gaccccggca cggccgatgg cacgcaccga agcagcgatg gccaccccgt
      121 tgagctctgc ggagcgcagc aggatcgcat ccggatcggc gagcgcgctg ccgacctcat
      181 agcggtccgc cggcaggcgg ccgaggccgc gcgggctgat ctgattcagg gtgaggacgc
      241 gcagcggcat gccggcatca gccgtggcgg cgcgcgaagt cgcgcatgaa tgccaccagc
      301 gcctccaccc ccgcgagcgg catggcgttg tagagactcg cgcgcattcc tcccaccgag
      361 cggtgcccgg cgagattggc gagcccggcg gcaagcgcac cggcgaggaa cggctcatcg
      421 agccgcgcat cggcgagcgt gaacggtacg ttcatccacg agcggcaatt gacggccacc
      481 ggattactgt agaagccgga ggagtcgatg gtcgcgtaga gcagttgcgc tttggcgcgg
      541 ttgtgtgcgg ccatcgccgc gagtccgccc tgggccttca gccagcgcag cacgaggccg
      601 gcaacatacc aggcgaaggt ggcgggcgta ttgagcaggg agccctcctg cgccatggca
      661 tggtagtccc acaccgccgg ggtgccggga cgcgcatggc ccgtgaggtc atcgcgcacg
      721 atcaccacca cgaggcccgc cggtccgata ttcttctgcg ccccggcgta aatcagcccg
      781 aacttcgcca cctccagcgg ccgcgagaga agcgtcgagg acatgtccgc gaccaggggc
      841 acgctgccgg agtcggggac gtagggaaac tcgactccgc cgatagtctc attgggcgtg
      901 tagtgcacat aggcggcatc gggggtgagg cgcaatgcct gctgcgttgg cacgctggtg
      961 tacctggagg cggcttcgtc ggcggcgatg ttgacgcgcg cggtgtagcg ctgcgcctcg
     1021 cgcagtgcct tgctcgacca gtgcccggtg ttgatgtaat cgacggtgga gtcggcccgc
     1081 gcgagattga gcggcacggc ggcgaattga ccgctggcgc cgccctggag aaacaacacc
     1141 cggtagtgac ttggcacacc cagaagctcg cgcaaaagcg cttccgactc ctgcgccacg
     1201 gccatgaagg ccttgccgcg gtggctcacc tccatgaccg aggttccgct gccctgccag
     1261 tcgagcagct cgtcccggac ctgttggagg acttccacgg gcaacgtggc cggccccgca
     1321 gcgaagttga atacgcgcac gaggcggcgc gcctagccgt ccgcggctgg tggggtcgca
     1381 cccggcgccg gctcgacgag cggctcctcg tcatcgccat ccaggctctc gatgcgctcg
     1441 atgccgacca gccgctcgcc ttcgccaagg cgtatcagcc gcacaccctg cgtgttgcgc
     1501 ccctgcacgg agatctcatc caccgtggtg cgtaccagcg taccggtgga gctgatcaac
     1561 atgatctcct gccccggtat cacctgcagc gccgccaccg tgaagccgtt gcgatcggag
     1621 gtctgcagcg cgatcacgcc ctggccgcca cggccgtgac aggggaactc cgccagcggg
     1681 gtgagcttgc cgtatccggt ggcggaggcg gtgagcacgc gcggcgcatc gtccgtgccg
     1741 ctgccggcgc cctcgccggc cacgatgagc gcgatcacgt cctggcccgc gccgagcttt
     1801 atgccgcgca cgccggccgc ctcgcgcccc atcggacgca cctcgctctc gtggaagcgg
     1861 atggccttgc cgccactcga caccagcatg atctcgcgct cgccgtcggt gagcgcgacg
     1921 cccaccagct gatcgttggg ggcgagatcg atgccgatga tgccggtcgt ccgcggccgc
     1981 gagaaggcgg cgagcggtgt cttcttcacc gtgccggcgc gcgtcgccat gaagacgaag
     2041 tgctgctcgt cgtactgctt gaccggtagc agcgcgttga tcttctcgcc ctcctcgagc
     2101 ggcagcaggt tgaccatcgg cttgccgcgc gcgccgcggc ccgcctgggg cagctcgtac
     2161 actttcagcc agtacacctt gccgcggttg gagaagcaca gcaccgtgtc atgcgtgtgc
     2221 gcgacgaaca gcttttcgac gaagtcctca tccttcaccg cggtcgcggt cttgccgcgg
     2281 cccccacgcc gctgtgtctg gtattccgtg agcggctggc tcttggcata gccggcgtgc
     2341 gagagcgtca ccaccacatc ctgcggctcg atgaggtcct cggtggtgag atcgagatga
     2401 tcgcggttga tctcggtgcg gcgcgcatca ccgtagctgt cgcgcacttc gatgagctcg
     2461 gcccgcacca cctgggtcag ccgctcggga cgcccgagga tatcgctgag atcggtgatg
     2521 ctggcgagca gctcgcggta ctcggcgatg atcttgtcct gctcgagtgc ggtcaggcgg
     2581 tgcagccgca tgtcgagaat cgcctgcgcc tgcacctccg atagccgata gctgcccgcg
     2641 tgcagaccgc tgtcggcggc aagtcccgga gggcgcgtcg atat
//
LOCUS       DATDMP010000493         2632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_746398, whole genome shotgun sequence.
ACCESSION   DATDMP010000493 DATDMP010000000
VERSION     DATDMP010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746398"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VI195_08670"
     CDS             <1..468
                     /locus_tag="VI195_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824500.1"
                     /translation="GFGSYVLLLPNRGVGIFAFANRTYAGPSAAVWDAAIALEKAGVL
                     GRERAVPVSADLARAYSAAGAIYTAGDIMAGGDVLAMNFILDRSADGWARDLKKLKAQ
                     VGNCETSEPPTASGELTGDFTWRCTHGRLKGSLELAPTRPPRIQWLEFEPIKP"
     gene            complement(524..>2632)
                     /locus_tag="VI195_08675"
     CDS             complement(524..>2632)
                     /locus_tag="VI195_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6824501.1"
                     /translation="SDLVDLTKEVVQRNLLRGMLLVVVVLIFFLYDVRAGLIVAASIP
                     LALMFAFICLDLQNASANLLSIGAVDFGILVDGAVVMVENIFRQMAARQGGPHDVVQI
                     IKDAAAEVDRPLVYAVAVIVAGFLPIYALSGPSGKLFKPMADTMVFALIGSLLVTLTL
                     LPVLCSWLMRPGAQERRNAGFEFIKTHYIRGLDACLARPWRTTFATVVLLALALLLMP
                     YVGAEFMPKLDEGALWVRATMPYTISFDEAAKIAPQVRGLLRTFPEVTTVASELGRPD
                     DGTDPTGFFNVEFYVGLKPYSQWHGRYRDKAGLIEAVNQKLAAFPGITFNYTQPAEDA
                     VDEAETGLKSALAVKIFGSDLHTLEQKGKEIKTVLERVRGIRDVTLVRELGQPSLTIS
                     VDRARIARYGLNVADINALVQTAIGGDVATQVEQGEKQFDLIVRLDQPYRDNPDEIGN
                     ILVATPGGQQIPLKEFADIQVTNGASFIYRENNSRYIGVQFSVTGRDLAGAVDDAMQQ
                     VSRSVALPQGYRLDWGGEYSDYTASRAQLRVILPLTLFLIFLLLFALYSNLKFPLITV
                     LGVLLSAPVGGIVALWLTGTPFSVSSGIGFLALFGVSVQTAVVYISYVNELRRDGTEL
                     AEAIREGAILRLRPIMMTALVAAFGLLPVALATGVGTDSQRPFALVIVSGLFTRLLIS
                     VFLMPVLYKLVSRPGDRLEV"
BASE COUNT          523 a          842 c          826 g          441 t
ORIGIN      
        1 ggctttggct cgtatgtact gttgctgccg aaccgaggcg tagggatctt cgcttttgcg
       61 aaccgcactt acgccgggcc atctgccgcg gtttgggatg ctgccatcgc actggagaag
      121 gccggcgtgc tcggtcggga gcgtgcagtg ccggtcagcg cggatctcgc aagggcctat
      181 agcgcggccg gcgcgatcta tacggcgggt gacatcatgg caggcggcga cgtattggcg
      241 atgaacttca tacttgatcg atcggcggat ggctgggccc gcgacctcaa aaagctaaag
      301 gcacaagttg gaaactgcga gacctcagag ccgccgaccg cttccggcga gttgacgggc
      361 gacttcacct ggcgctgcac gcacggccgc ttgaagggca gcctggaact ggcacccacg
      421 cgcccgccgc gcatccagtg gctggagttc gagccgatca agccttgatg gcggccctcg
      481 tttgttgctc ctgaacggtg cgctgtagcg ccaggatcgc ccctcacact tcgagtcgat
      541 cgccggggcg gctcaccagc ttatacaaca cgggcatcag aaagacgctg atcaacagtc
      601 gagtgaatag cccgctgacg atcaccaggg caaacggtcg ctgcgagtcg gtgccgacgc
      661 cggtagccag cgcgaccggc agcagcccga acgccgccac cagcgcggtc atcatgatcg
      721 gtcgcaaccg cagtatcgcg ccctcgcgga tggcctcggc gagctcggtg ccgtcgcggc
      781 gcagttcgtt gacataggag atgtacacga ccgcggtttg caccgacacg ccaaacagcg
      841 cgagaaagcc gatgccggag gacaccgaga acggcgtgcc ggtgagccac agcgccacga
      901 ttccgcccac cggcgccgac aacagcacgc ccagcacggt gatgagcggg aacttcagat
      961 tgctgtagag cgcgaacagc aggaggaaaa tgagaaatag ggtgagcggc aggatgacac
     1021 gcagctgcgc gcgcgaggcg gtgtaatcgg agtactcacc gccccagtcc aggcgatagc
     1081 cctgcggcaa ggcaacgcta cggctcacct gctgcatggc atcgtctacg gcgccagcca
     1141 gatcccgtcc cgtcaccgaa aactgcacac cgatgtagcg cgaattgttc tcgcgataaa
     1201 tgaacgaggc gccgttggtc acctgaatgt cggcgaactc cttgagcgga atctgctggc
     1261 caccgggggt ggcaaccagg atattgccga tctcatccgg gttgtcgcga tatggctggt
     1321 cgaggcgcac gatgagatca aactgctttt ctccctgctc tacctgggtc gccacatccc
     1381 cgccgatagc cgtctgaacc agcgcattga tgtcggcaac gttgaggccg tagcgcgcga
     1441 tccgggcgcg gtcgacggag atggtcaggc tcggctgccc gagctcgcgc accagcgtga
     1501 catcgcggat gccgcgcacc cgctccagga ccgtcttgat ctccttgccc ttctgctcga
     1561 gcgtgtgcag gtcggacccg aagatcttga ccgccagcgc gctcttcagt ccggtctccg
     1621 cttcgtccac ggcatcctcg gccggctgcg tgtagttaaa ggtgatcccg gggaatgccg
     1681 cgagcttctg gttgaccgcc tcgatcaggc ccgccttgtc ccgatagcgg ccgtgccact
     1741 gcgaataggg cttgagcccc acatagaact cgacgttgaa gaacccggtg gggtcggtac
     1801 catcgtcagg ccggccgagc tccgaggcta ccgtcgtcac ttcgggaaat gtgcgcagca
     1861 gcccccgcac ctgcggagcg attttcgcgg cctcatcgaa ggagatggtg tagggcatgg
     1921 tggcgcgcac ccatagcgca ccctcgtcca gcttcggcat gaattcggcg ccgacgtacg
     1981 gcatcagcag cagtgcgagc gcgagcagca cgaccgttgc gaaggtcgtt ctccacggcc
     2041 gcgcgagaca tgcatccagg cctctgatgt agtgcgtctt gatgaattcg aagccggcat
     2101 tgcgccgctc ttgtgcgccc ggacgcatga gccacgaaca cagcaccggt aacagcgtca
     2161 gcgtgacgag cagcgaaccg atgagcgcga acaccatggt gtccgccatc ggcttgaaca
     2221 gcttcccgga ggggcccgag agcgcataga tcggcaggaa cccggctacg atcaccgcga
     2281 ccgcgtaaac cagcggacgg tccacttcgg ccgccgcatc cttgatgatc tgtaccacat
     2341 cgtgcggccc gccctggcgc gcggccattt ggcggaagat gttctccacc atcaccacgg
     2401 cgccatccac cagaatgccg aaatccacgg ccccgatgga cagcagattg gccgaggcat
     2461 tctgcagatc gaggcaaatg aacgcgaaca tcaatgccag cggaatgctc gcggcgacga
     2521 tcaacccggc gcgcacgtca tataggaaga agatcagcac cacgacgacc agcagcattc
     2581 cgcgcaacag gttgcgttgg acgacttcct tggtcaggtc gaccagatcg ct
//
LOCUS       DATDMP010000494         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1968085, whole genome shotgun sequence.
ACCESSION   DATDMP010000494 DATDMP010000000
VERSION     DATDMP010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968085"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VI195_08680"
     CDS             <1..186
                     /locus_tag="VI195_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6824502.1"
                     /translation="LKEHRELGPPNTVYVQTRDGVVYLTGQVSTDLQRGTAESIAGKA
                     PGVVQVVNSIALTFGGR"
     gene            261..1880
                     /locus_tag="VI195_08685"
     CDS             261..1880
                     /locus_tag="VI195_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY6824503.1"
                     /translation="MQSQETGQGAADVSAQIARAVAAYRAPARAPSVDHGAAAALREV
                     YAQRANVPLWSRDGQATAQAQAVVHALQRADTYGLQPEDYAGNALAKLADEPPAAASG
                     NAERWGRFDVRLSAATLRFLSDLHYGRVQPAAAGFNLQGTRQPLDLAASLTTLAAASD
                     VSETLSRVEPPFHHYALLKQALAKYLDLAQHPELKHLPAISGSLKPGDPYAGAMALRR
                     LLIALGDMPASTAPSADDSIFSPELSEGVRTFQQRHGVSTDGVLGKATLVALTTPFTK
                     RVRQIDLTLERWRWLPPFATPPIIVNIPQFRLFAFRTTDDRVADILQMNVIVGKPYPR
                     MQTPVFESDMRYVVFRPYWDVPPSILRNEMLPKIRANPEYLAAQRLQIVNGQGDDSPV
                     VPPSPEAIEALAAGKLRVRQMPGEDNALGLVKFMFPNSYNVYLHSTPVHGLFKESVRT
                     FSHGCIRVSDPLALATHVMRDEPGGWTREKIDATMNGAKPVRVNLQRPITVLILYGTA
                     LATEAGPIEFFQDIYGYDRKLEQQLGLPLMR"
     gene            complement(1891..2451)
                     /locus_tag="VI195_08690"
     CDS             complement(1891..2451)
                     /locus_tag="VI195_08690"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEY6824504.1"
                     /translation="MTAILHRHKPDRSDRRRFLQCAGAAVMLLPLDAAWARAPERRSL
                     SFVHTHTGERLSSVYFEAGEYRASELERINQLLRDFRTGDVHPIDTGVLDILADLRML
                     ADRDEPYEVISGYRSPQTNAALREHSSGVAEHSLHLQGRAIDVRLPGFPTGKLRELAL
                     GMGRGGVGFYARPDFVHLDNGRVRCW"
BASE COUNT          456 a          865 c          848 g          403 t
ORIGIN      
        1 ttgaaagagc atcgcgagct cggtcctccc aacaccgtct acgtgcagac gcgcgacggc
       61 gtggtctatc tcaccggcca ggtgtcaacc gacctgcagc gcggcaccgc ggagagcatc
      121 gccgggaagg cgccgggcgt cgtgcaggtg gtgaactcca tcgcgctgac attcggcggt
      181 cgctgatgcg ccgcccgccg cccggcagcc agttggctgt atggctggtt gcgatatgcg
      241 cgctcgttca ttcgggagcg gtgcagtccc aggagaccgg gcagggtgcc gcggacgtgt
      301 cggcacagat cgccagagcg gtggccgcgt accgggcgcc ggcgcgcgcg ccatccgtcg
      361 accatggcgc cgcggcggcg ctgcgcgagg tctatgcgca gcgagccaat gtcccgctgt
      421 ggagccgcga cggacaggcg accgcgcagg cgcaggcggt ggtacacgcc ctgcagcgtg
      481 ccgataccta tgggttgcag cccgaggatt acgccggcaa cgcgctcgcg aagctcgcgg
      541 atgagccgcc ggccgccgcc agcggcaatg ccgagcgctg gggccgtttc gatgtgcggc
      601 tctcggcggc aacgctgcgc ttcctgagcg atctgcacta cggacgcgtg cagcctgcgg
      661 cggcgggctt caatcttcag ggcacccggc agccgctcga cctggccgcg agcctcacga
      721 ccctcgcggc tgcgagcgat gtgagtgaga ccctgagcag ggtcgagccc cccttccatc
      781 attacgcgct gctcaagcaa gcgctcgcca agtacctgga tctcgcgcaa cacccggagc
      841 tgaagcatct gcctgccatc agcggctcgc tcaagccggg cgatccgtat gccggagcga
      901 tggcgctacg gcggttgctc attgcgctcg gcgacatgcc ggctagcacc gccccgagcg
      961 cggatgacag cattttcagt cccgagttga gcgagggcgt gcgtaccttt cagcagcgcc
     1021 acggcgtgag cacagacggg gtgctgggca aggccaccct ggtggcgctg acgaccccgt
     1081 tcacgaaacg ggtgcgccag atcgacctga cgctcgagcg ctggcgctgg ctaccgccgt
     1141 ttgcaacgcc accgatcatc gtcaacattc cgcaattccg cctgttcgcg tttcgcacca
     1201 ccgacgaccg tgtcgccgac attctgcaga tgaatgtcat cgtcgggaaa ccctacccac
     1261 gcatgcagac cccggtgttc gagtcggaca tgcgctacgt ggtgttcagg ccgtactggg
     1321 acgtgccacc cagcatcctg cggaacgaga tgctaccgaa gatacgcgcc aatccggaat
     1381 atctcgccgc gcagcggctg cagatcgtga acggtcaggg cgatgactcg cccgtggtgc
     1441 ccccctcacc tgaggccatc gaggcgctgg cggcgggcaa actgcgggtg cgacagatgc
     1501 ccggtgagga caatgcgctc gggctcgtga agttcatgtt tcccaacagc tacaacgtct
     1561 acctgcactc gacccccgtt cacgggctgt tcaaggagtc ggtgcgcacc ttcagtcatg
     1621 gctgcatccg ggtgagcgat ccgctggcgc tcgcgaccca cgtgatgagg gacgagcctg
     1681 gcgggtggac acgcgagaag atcgacgcga ccatgaacgg cgcaaaaccc gtacgggtga
     1741 atctacaacg cccgatcacg gtgctgattc tctacggcac ggcgctcgca accgaggccg
     1801 gaccgatcga gtttttccag gatatctacg gatacgacag gaaattagag cagcagctgg
     1861 gtctgccgct catgcgctag gggcacacca ctaccagcag cgcacccgcc cgttatccag
     1921 gtgcacgaag tcggggcgcg catagaaacc gaccccccct cgacccatcc ccagcgcgag
     1981 ctcgcgcagc ttgccggtcg ggaaccccgg cagccgcacg tcgatcgcgc gcccttgcag
     2041 atggaggctg tgctcggcaa ccccgctcga gtgctcccgc agtgctgcat tggtctgagg
     2101 cgagcggtaa ccgctgatca cctcgtaagg ctcgtcgcga tcggcgagca tgcgcaggtc
     2161 cgcaaggata tcgagcactc cggtgtcgat ggggtgcacg tcaccggtgc gaaagtcgcg
     2221 cagcagctga ttgatgcgct cgagctcgct ggcgcggtac tccccggcct cgaagtagac
     2281 gctcgacaga cgctcgcccg tatgggtgtg cacgaacgac agcgagcgac gctcgggggc
     2341 acgcgcccac gcggcgtcga gcggcagcag catgacagcg gcaccggcgc actgcagaaa
     2401 ccggcgccgg tccgagcgat ccggcttgtg ccgatgaaga attgccgtca cgcggccatg
     2461 gctcccattg ggcaggcgcc gcatcttacc caagaacagc cgagcggcga caggtcgccg
     2521 atcacaagcc gcccgagggg ccttggctca gcggtttacc attaatcggc cg
//
LOCUS       DATDMP010000495         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1302199, whole genome shotgun sequence.
ACCESSION   DATDMP010000495 DATDMP010000000
VERSION     DATDMP010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302199"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="VI195_08695"
     CDS             complement(<1..2018)
                     /locus_tag="VI195_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HEY6824505.1"
                     /translation="MASRSALTTAHAAGSPPGDTSVAAAIADEALRLEFDSAMRSRTW
                     HVGRNGPPSTLALTSWSETEYLLLADGARLDGFALEHQERAEIRGPHGEGKHLTLSGR
                     NAAGVEKTVRIEMWRRYPGVAFYRVSYRNRSPRAFALRGWRSAELELLPEAEPRAAAA
                     RKEEPLFWSYCGSTHADRRDWVQPVRQGFEQENFMGMTASDYGGGIPVVDVWRRDCGI
                     ALGHLEPVPRLLSLPVKRVAGGAKLAIAGTLERDLPSGARFETPETFLATHTGDYFAT
                     LVAYRRVMAERGLVSPHAPDSAYESIWCAWGYERECTPQLIEGTLPKVRELGLRWAVI
                     DDGWQSNVGDWKPHPGKFPQGAGDVRQLVRDIRAKELKPRLWFAPLATAPGSDLLHDH
                     TDMLLLDKEGTPQNISWWNCFYLCPAYAPTVEYTQTLVRRFIGDWGFAGLKIDGQHLN
                     GVAPCYNPAHRHARPEESVEKLQDFMHALYSAAVQIDPQAVMELCPCGTAYSFFNFPS
                     MNQAPASDPESSWQVRLKGKTLKALMGASAAFAGDHVELSDGGDDFASTVGIGAIVST
                     KFTWPRDPKPKDSFLLTPAREALWRKWIALYNERMLPKGTYRGELYDIGFDKPETHVV
                     ETAGGLYYGLYARSWSGPVTLRGLAHGRYRLRDYFNDRELGEVSAPGAT"
BASE COUNT          351 a          686 c          686 g          327 t
ORIGIN      
        1 gtggcgcccg gcgcgctcac ctcgcccagc tcacgatcgt tgaagtaatc gcgcaatcga
       61 taacggccgt gcgcaagccc gcgcagggtc accggaccgc tccagctgcg ggcgtagagg
      121 ccgtagtaca gcccgccggc ggtctcgacc acgtgcgtct cgggcttgtc gaagccgatg
      181 tcgtagagct ccccgcgata ggttcccttc ggcagcatcc gctcgttgta gagcgcgatc
      241 cacttgcgcc acaacgcttc gcgcgccggt gtcagcagga aagaatcctt gggcttggga
      301 tcccgcggcc aggtgaactt ggtcgatacg atcgcgccga tgccgacggt ggaagcgaag
      361 tcatcgccgc catcgctcaa ctccacgtga tcgccggcga aagcggcact cgcacccatg
      421 agcgccttga gcgtcttgcc cttcaggcgc acctgccacg aggactccgg gtccgaggcc
      481 ggagcctgat tcatggatgg gaaattgaag aaggagtagg cggtcccaca cggacacaac
      541 tccatgacgg cttgcgggtc gatctgcacg gcagcgctgt acagcgcgtg catgaaatcc
      601 tgcagcttct cgaccgattc ctcagggcgc gcgtgccggt gtgcggggtt gtaacagggc
      661 gcgacgccat tgaggtgctg accgtcgatc ttcagaccgg cgaagcccca gtccccgatg
      721 aagcggcgca cgagcgtctg ggtgtactcc acggtcggcg cataggcggg gcacaggtag
      781 aagcagttcc accaagagat gttctgcggg gtgccctcct tgtcgaggag cagcatgtcg
      841 gtgtgatcgt ggagcaggtc cgagccggga gcggtggcga gcggtgcgaa ccatagccgc
      901 ggcttgagct cttttgcacg gatgtcccgc accagctgcc gcacatcgcc cgcaccctgc
      961 ggaaacttcc ccgggtgcgg cttccagtcg ccgacattcg attgccagcc gtcatcgatg
     1021 acggcccaac gcaagcctag ctctctcacc ttcggcagcg tgccctcgat cagttgcggc
     1081 gtgcattcac gctcgtagcc ccacgcgcac cagatggact cgtacgccga gtcaggggca
     1141 tgtggagaaa cgagaccgcg ctcggccatg acgcggcgat aggcgacgag cgtcgcgaag
     1201 tagtcgccgg tgtgcgtggc caggaatgtc tcgggagtct caaagcgcgc cccggacgga
     1261 aggtcgcgct cgagtgtccc ggcgatcgcg agcttcgcgc ccccggcaac acgcttcacc
     1321 ggcagtgaca gcagccgcgg caccggttcc aggtgcccga gcgcgatacc gcagtcgcgg
     1381 cgccagacgt ccacgaccgg tatgccgccg ccgtagtcgg aggcggtcat ccccatgaag
     1441 ttctcctgct cgaagccctg tctcaccggc tgcacccagt cgcgccggtc ggcatgcgtg
     1501 ctgccacagt aggaccagaa cagcggttcc tctttgcgag cggctgccgc gcgaggctcg
     1561 gcttctggca acagctcgag ctcggcgctg cgccagcctc gcaacgcgaa cgcacgcggc
     1621 gagcggttgc ggtaggacac gcggtagaac gcaacgcctg gatagcgccg ccacatctcg
     1681 atgcgcacgg tcttctcgac accggcggcg ttgcggcccg agagcgtgag gtgcttgccc
     1741 tcaccgtggg gaccgcgtat ctcggcgcgc tcctggtgct cgagagcgaa cccatcgagc
     1801 cgtgcgccat cggcaagcag caggtactcc gtctcgctcc acgaggtgag cgcgagcgtc
     1861 gacggcggcc cattgcgccc gacatgccag gtacgcgagc gcatcgcgga gtcgaactcg
     1921 agacgcaagg cctcgtcggc gatcgcggcc gctaccgagg tgtcgcccgg cggtgaacct
     1981 gcggcgtgcg ccgtggtgag cgcgctgcgc gaggccaagc cggcgcccgc ggccagttgg
     2041 agaaatcgac
//
LOCUS       DATDMP010000496         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_191086, whole genome shotgun sequence.
ACCESSION   DATDMP010000496 DATDMP010000000
VERSION     DATDMP010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191086"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..>2486)
                     /locus_tag="VI195_08700"
     CDS             complement(219..>2486)
                     /locus_tag="VI195_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6824506.1"
                     /translation="VEAKRFAELSIGSVLLISIRVSGQPAGILGLANALPRGAWDVNL
                     QLLMKLIGTSLATGLERIRTQARLAKLEERTNLFQAAANDGLWDFDVESNEVFFSPRW
                     KAMLGYGDDDMRGSPDWRSLVHPDDLARVQAAIRDHVAGKTPIFESTHRMRHRNGEWR
                     WVISRATARVDKHGRLLRLVGVELDITERKLYEEALFREKESAQITLQSIGDGVITTD
                     ASSTIDYINPVAEELTGWRLEDAMGRPVEEIFRAFHEETCEPLENPLSVSIRRIRPIK
                     SVRPMLLIRRDGNELYVESTAAPIRDGSGHVAGGVLVFHDVSESRELNRRLSYHASHD
                     LLTGLVNRREFESRLERALKSAKAREASYALCYLDIDQFKIVNDTCGHSAGDALLGQV
                     GALLKSKVRWRDTLSRLGGDEFGILLESCSLDEAMRTAETLREGVRNFRFTWEDRVFR
                     LGASIGVVPITADNEDVASILSAADSACAAAKESGRNRVHSFAENDIELMRRRREMQW
                     AARINAALEEGRFELFRMAIQPLQRPETGAHYELLLRLKDETGRIVAPNDFIAAAERY
                     GITPAIDRWVIENAFRWLVSEADEREKLLQCSINLSGQSLGDDKFLPFVIDQFHRSGL
                     DASKICFEITETAAVASFSQANRFIQALKELGCKFALDDFGTGLSSFGYLKHFPVDYL
                     KIDGSFVREILRDPIDREMVRSINEIGHLTGKQTIAEFAENAEIIQMLTSLGVDYAQG
                     YGVAQPQRVLKSAVA"
BASE COUNT          416 a          797 c          853 g          420 t
ORIGIN      
        1 cgagcgtgaa cgcccaaaac cccaggtacc tgccgaagct ccgcagggag ccttccgagc
       61 gggcggagag gtacaggaca aaatagccgg cgatggtgag tgcagtcgcc ggaagcagcg
      121 ccaggaggta gatcatgaga caccggtcgg tcggcaatcg atgggtaaat ggtactcccg
      181 ccggcgcgac aggaacgacc gctcgtctcg gtgaggcctc aggcgaccgc ggacttcagc
      241 acgcgctgcg gctgagcgac gccgtagccc tgcgcgtagt cgaccccgag gctggtgagc
      301 atctgaatga tctcggcgtt ctcggcgaac tccgcgatcg tctgtttgcc ggtgaggtga
      361 ccgatctcgt tgatcgagcg caccatttcg cggtcgatcg gatcgcgcag gatctcgcgt
      421 acgaagctgc cgtcgatctt gaggtaatcg accgggaagt gcttgaggta accgaacgag
      481 gagagcccgg tgccgaagtc atcgagcgcg aacttgcagc cgagctcctt cagcgcctgg
      541 atgaagcggt tcgcttgtga gaagctcgcg accgccgccg tctcggtgat ctcgaagcag
      601 atcttcgagg catcgaggcc gctcctgtgg aactgatcga tgacgaaggg caggaacttg
      661 tcatccccga ggctctggcc ggagaggttg atcgagcact gcagcagctt ctcgcgctcg
      721 tccgcctccg agaccagcca gcggaaggcg ttctcgatca cccagcggtc gatcgccggg
      781 gtgatgccgt agcgctcggc ggcggcgatg aagtcattcg gcgccacgat gcgcccggtc
      841 tcatccttca ggcgcagcag caactcgtaa tgcgcacccg tctcgggacg ctgcagcggc
      901 tggatcgcca tgcggaagag ctcgaagcgc ccctcttcga gcgccgcgtt gatgcgcgcc
      961 gcccactgca tctcgcgccg ccgccgcatg agctcgatgt cgttctcggc gaagctgtgg
     1021 acgcgattgc ggccgctctc cttggcggcg gcgcaggcgg aatcggcggc cgagaggatc
     1081 gaggccacgt cttcgttgtc ggcggtgatc ggcacgaccc cgatgctcgc accgaggcgg
     1141 aatacccggt cttcccaggt gaagcggaag ttgcgcacgc cttcgcgcag ggtctcggcg
     1201 gtgcgcatcg cctcatcgag cgagcagctc tcgaggagga tgccgaactc atcgccgccg
     1261 agccgtgaga gggtatcgcg ccagcgcacc ttggatttca gcagcgcgcc cacctgtccg
     1321 aggagcgcat cgccggcgga atgcccgcag gtgtcattga cgatcttgaa ctggtcgatg
     1381 tcgaggtaac agagcgcgta ggaggcctcg cgcgccttgg cgctcttcag cgcccgctcg
     1441 aggcggctct cgaattcccg ccggttcacg agtccggtga gcagatcgtg gctcgcgtgg
     1501 taggagaggc ggcgattgag ctcgcgcgac tcgctcacat cgtggaacac cagcacgccg
     1561 cctgccacgt ggcccgagcc atcgcggatc ggcgcggcag tgctctcgac gtacagctcg
     1621 ttgccgtcgc gccggatcag cagcatcggt cgcaccgact tgatggggcg gattcggcgg
     1681 atcgacaccg agagcggatt ctcgagcggc tcgcaggtct cctcgtggaa ggcgcggaag
     1741 atctcctcca ccgggcgccc catggcgtcc tcgagccgcc agccagtgag ctcctcggcc
     1801 accgggttga tgtagtcgat ggtggatgag gcgtcagtgg tgatgacacc atcgccgatc
     1861 gactgcagcg tgatctgcgc gctctccttc tcgcgaaaca gcgcctcctc gtagagcttg
     1921 cgctcggtga tgtcgagctc gacccctacg aggcgcagca gccgcccgtg cttgtcgacg
     1981 cgggccgtgg cgcggctgat aacccagcgc cactcgccgt tgcggtggcg catgcgatgg
     2041 gtgctctcga agatcggggt cttgccggcg acgtggtcgc ggattgcggc ctgcacgcgc
     2101 gccaggtcat cggggtgcac gaggctgcgc cagtcgggcg agccgcgcat gtcgtcatcg
     2161 ccgtagccga gcatcgcctt ccagcggggc gagaagaaga cctcgttgct ctcgacgtcg
     2221 aagtcccaca agccatcatt ggccgccgcc tggaacagat tggtgcgctc ctcgagcttg
     2281 gcgagccgcg cctgggtgcg gatgcgctcg aggccggtgg cgaggctcgt gccgatgagc
     2341 ttcatcagca gctgcaggtt cacgtcccag gcgccgcgcg gcagcgcatt ggcgaggccc
     2401 aggatgccgg cgggctgccc gctcacccgg atggaaatca gcagcaccga gccgatggaa
     2461 agctcggcga agcgcttggc ctccac
//
LOCUS       DATDMP010000497         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_968790, whole genome shotgun sequence.
ACCESSION   DATDMP010000497 DATDMP010000000
VERSION     DATDMP010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968790"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /gene="ribB"
                     /locus_tag="VI195_08705"
     CDS             <1..1015
                     /gene="ribB"
                     /locus_tag="VI195_08705"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY6824507.1"
                     /translation="EHATAEAVAFMIRHTSGIICVPMEEAALVRLDLPQMVPVNSESQ
                     RTAFTVSVDLRVGTTTGVSSVDRAATIRALADRSSTAAEFARPGHIFPLRSRRGGVLV
                     RAGHTEAAVDLCRLAGLTPMGVLCEVMNDDGSMARRPQLQEFARVHGLKIGTIADLIR
                     HRLRTERTVVRISEQSVQTELGEFRLYAYQDEVSLEVHVALAHGRLDGAEPPLVRVHV
                     ADTLRDLLGVRATARAWTLRAAMQRIVEAGNGVVVVLRQQESARELAAALRSFAGPTT
                     AAAADGAAEAQEGTVLRTFGVGAQILKDLGVKRMRVLSAPKQMHGISAFGLEVESYVG
                     DEP"
     gene            1012..1479
                     /gene="ribH"
                     /locus_tag="VI195_08710"
     CDS             1012..1479
                     /gene="ribH"
                     /locus_tag="VI195_08710"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY6824508.1"
                     /translation="MSAELIEGEVSARGRRVAIVAARFNDFIVASLLKGARAAWHQRG
                     GSADDLTVVRVPGAFELPVVVRRLAASGRYDAVVALGCVIRGDTPHFDYVAGEAARGL
                     QLAGLETGVPVAFGVLTVDSLEQALERAATSAGNKGGEAMESALEMAAVMARL"
     gene            1491..1931
                     /gene="nusB"
                     /locus_tag="VI195_08715"
     CDS             1491..1931
                     /gene="nusB"
                     /locus_tag="VI195_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY6824509.1"
                     /translation="MSPQGTHAYSGTRSVARKLALQALYRWQLNAAPWQDLVQEFGEA
                     QDMSRADADYFRTLVEGVWRGREELDARLATFADREPRLLDPVERAALLIALYELTAR
                     PEVPYRVAINEAVTLAKRFGATDGFKFVNAVLDRAARALRPDEH"
     gene            1947..>2305
                     /locus_tag="VI195_08720"
     CDS             1947..>2305
                     /locus_tag="VI195_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY6824510.1"
                     /translation="MALSEFALIERYFRGRGAERTDVTLGVGDDAALMRVPPGCELVA
                     ATDTLVAGVHFPQDSPPESIGHRALAVNLSDLAAMGARPAWALLALTLPQADEAWLGG
                     FATGLGELARTYEVALVG"
BASE COUNT          316 a          770 c          858 g          361 t
ORIGIN      
        1 cgagcacgcc acggccgagg cggtggcgtt catgatccgg catacgagcg gcatcatctg
       61 cgtgcccatg gaggaggccg ccctcgtgcg gctcgatctg ccacagatgg tgccggtcaa
      121 cagcgagtcg cagcgtaccg ccttcaccgt gtcggtggat ctgcgggtgg gcacgacgac
      181 cggggtgtcc tcggtcgatc gcgccgccac catacgcgcg ctcgccgacc gcagctcgac
      241 cgcggcggag ttcgcgcgcc cgggacacat ctttccgctg cgctcgcggc gtggcggcgt
      301 gctggtgcgc gccggccaca cggaagcggc ggtcgatctg tgccggctcg cggggcttac
      361 gcccatgggc gtgctgtgcg aggtgatgaa tgacgatggc agcatggcgc gccgtccgca
      421 gttgcaggag ttcgcgcgcg tgcacgggct gaagatcggc acgatcgccg atctcatccg
      481 gcaccggttg cgcaccgagc gcaccgtggt gcgcatctcc gagcagagcg tgcagaccga
      541 gctcggcgag tttcgcctgt acgcgtatca ggacgaggtg agcctcgagg tgcacgtggc
      601 gctcgcccac gggcggctcg atggtgcgga gcccccactg gtgcgcgtgc atgtcgccga
      661 caccttgcgt gacctgctcg gcgtgcgcgc aaccgcgcgc gcctggacgc tgcgcgcggc
      721 gatgcagcgc atcgtcgagg cgggcaacgg ggtcgtggtg gtgctgcgtc agcaggaatc
      781 cgcgcgtgag ctcgccgcag cgctacgctc gttcgcaggt ccgacgacgg ccgctgcggc
      841 tgatggcgcc gccgaggctc aggaaggaac ggtactgcgc accttcggtg taggcgcgca
      901 gatcctcaag gatctcgggg tgaagcgcat gcgcgtgctg tcggccccga agcagatgca
      961 cggcatctcc gcctttggtc tcgaggtcga aagctatgtc ggggacgagc cgtgagcgcc
     1021 gagctcatcg aaggagaggt gagcgcgcgc gggcgacgcg tggccatcgt cgccgcgcgc
     1081 ttcaacgatt tcatcgtcgc gagcctgctg aagggggcgc gtgccgcctg gcaccagcgc
     1141 ggtggctccg ccgacgatct caccgtagtg cgtgtgcccg gtgcctttga gctcccggtc
     1201 gtggtgcgcc ggctcgcggc gagcggccgc tacgacgcgg tggtcgcact cgggtgcgtg
     1261 atccgcggcg atacaccgca cttcgactac gttgccggcg aggcggcgcg cggcctgcag
     1321 cttgcgggcc tggagaccgg tgtgccggtc gctttcggcg tgctgacggt ggactctctc
     1381 gagcaggcgc tcgagcgcgc cgccaccagc gccggcaaca agggaggcga ggcgatggag
     1441 agcgcactcg agatggcggc cgtcatggcg cggctgtgag cgccggctcg atgagcccgc
     1501 agggcacgca cgcttacagc ggtacgcgca gcgttgcgcg caagctcgcg ctgcaggcgc
     1561 tgtaccgctg gcagctgaac gccgcgccat ggcaggacct ggtgcaggag ttcggcgaag
     1621 cgcaggacat gtcacgggcg gatgccgact actttcgcac actggtcgag ggcgtatggc
     1681 gcgggcgcga agagctcgat gcgcggctcg ccaccttcgc ggaccgtgag ccgcgcctgc
     1741 tcgatccggt cgagcgcgcg gcgctgctga tcgcactcta cgagctcact gcccgccccg
     1801 aggttccgta ccgcgtggcg atcaacgagg cggtgacgct cgccaagcgc ttcggcgcca
     1861 ctgacggctt caagttcgtg aacgccgtgc tcgatcgcgc cgcgcgcgcg ctgcggccgg
     1921 acgagcactg aagggacctg cgtcccatgg cgctctcgga attcgcgctg atcgagcgct
     1981 acttccgcgg ccgcggcgcc gagcgcaccg atgtgacgct cggcgtcggg gacgatgcgg
     2041 cgctcatgcg tgtgccaccc ggctgcgagt tggttgccgc taccgataca ctggtcgcgg
     2101 gcgtgcactt tccacaggac tccccgcccg agtcgatcgg ccaccgggcg ctcgccgtca
     2161 acctgagcga tctggccgcg atgggcgcgc gcccggcttg ggcgctgctc gccctgacgt
     2221 tgccgcaagc ggatgaggcg tggctcggcg ggttcgcgac cggcctcggg gagctcgcac
     2281 gcacttacga ggtggcgctg gtcgg
//
LOCUS       DATDMP010000498         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_524862, whole genome shotgun sequence.
ACCESSION   DATDMP010000498 DATDMP010000000
VERSION     DATDMP010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524862"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VI195_08725"
     CDS             complement(<1..1379)
                     /locus_tag="VI195_08725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6824511.1"
                     /translation="MNQLAFAIGVLAGLACAAIGCALLWWLRFRRPASPYDAQHLAVF
                     EQWPTTAIALDPATMRWVAGNPASLRNLGYTLEELRELTFGDIFSVAGVEGRDLLLRL
                     QDATARAPLEMRQRCADGSERNVEATCYRIEVEGRQLLAVAVHDVTIRRKVETQLLAK
                     HQQLDHLAHHDHLTGLPNRLYLAAHLPDAIEEAKKNGSVLAVLFLDLDRFKHVNDSRG
                     HETGDKLLKTVAQRVRSTMRAEDLVVRMGGDEFVVVMRGVKDTEQVNEAATRINRALG
                     APMVVDGRTLVTTVSIGVALYPSDGADMGELLRHSDTAMYQAKDRGRNNFQLFSQSMD
                     KRLKERIAIESSLRTALQSGQLDVHYQPIVDIESQRVIALEALLRWKHPSHGYVRPER
                     FVSIAEEAGLIVPIGEFVLQRVIQNSVRWRQQGARIVPVAINVSAVQLQRTNLAESIS
                     RLTKEHGLEP"
     gene            complement(1486..2439)
                     /locus_tag="VI195_08730"
     CDS             complement(1486..2439)
                     /locus_tag="VI195_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY6824512.1"
                     /translation="MPVVALLEVFFARMPILAGGECCSIWQTASSRTHKVWPQMQRST
                     VASATISGLFVYPLKSGRAVSCARVRMSATGFEWDRQWMLINANGVFLSQRTHPQLAC
                     IVPQLDAGELLLEAPGVPRLRLPRVATGERRSVRVHRDMCVGLDEGEAAAQWASHIAG
                     EAARLVRVPARVERFANPAFAGTAPAPVGFADGFPVLVCNQASLEDLNTRLPQRIPME
                     RFRPNVVLEGLPAWAEDRIDALTVDGVTLRLVKPCSRCTIPSIDQQTGEPSTDPAPVL
                     RQFRFDKKLHGVTFGENAVLVSAERGEIVRGAVCEVSFDSA"
     gene            2438..>3397
                     /locus_tag="VI195_08735"
     CDS             2438..>3397
                     /locus_tag="VI195_08735"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY6824513.1"
                     /translation="MKGLVRTAVTAMVSASVIFGGPAAHSRTQKPPLYGRHWMAVTGK
                     PIAATAGAKVFAQGGNAVDAACAMLAAAATAWTTLSWGGETQALIYNPNTGRVIAING
                     LGVAPTGATPDYFHKLGMRYPPEYGPLAAVTPGTPGGLMTMLAEFGTMSLAQVLAPAI
                     EMADGFPMDEALAGTIEHFKDKLKEWPDSKRVMLPHLGSSHEGPMPGEIFRQPDLAVT
                     LRKLVEAEKQALKSGKDRKAAIYAAYERFYRGDIASDLVQAVRAAGGLFTLEDLAHWK
                     VHIEEPVHSSYKGIEVYKLNAWVQGPVMLQTLNILENFDLRSMG"
BASE COUNT          595 a         1123 c         1114 g          565 t
ORIGIN      
        1 ggctcgagcc cgtgctcctt ggtgagccgc gagatggatt ccgcgagatt ggtgcgctgc
       61 agctgcaccg ccgagacgtt gatcgcgacc ggaacgatgc gcgcgccctg ctgccgccag
      121 cggaccgagt tctggatcac ccgctgcagc acgaactccc cgatcggtac gatcaggccg
      181 gcttcctcgg cgatggacac gaagcgttcg gggcgtacgt aaccgtggct cgggtgcttc
      241 cagcgcagca gcgcctcgag cgcgatcacc cgctgcgatt cgatgtcgac gatcggctgg
      301 tagtgcacat cgagctgacc gctctgcagc gccgtgcgca ggctcgactc gatggcgatg
      361 cgctccttga ggcgcttgtc catgctctga ctgaagagct ggaagttgtt acggccgcgg
      421 tccttcgcct ggtacatggc ggtgtcggag tggcgcagca gctcgcccat gtcggcaccg
      481 tcgctgggat aaagcgccac accgatactg accgtggtca cgagcgtgcg tccgtctacg
      541 accatcggtg caccgagtgc gcggttgatg cgcgtggccg cctcgttcac ctgctcggtg
      601 tccttgacgc cgcgcatcac caccacgaac tcatcgcccc ccatgcgcac caccaggtct
      661 tcggcccgca tggtcgagcg cacccgttgt gcgaccgtct tcagcagctt gtcgccggtc
      721 tcgtggccgc gcgagtcgtt gacgtgtttg aaacggtcga ggtcgaggaa cagcacggcg
      781 agcacgctgc cgttcttctt cgcctcctcg atcgcatccg ggagatgcgc ggcgaggtac
      841 aggcgattcg gcagccccgt gaggtgatca tggtgggcga ggtgatcgag ctgctggtgc
      901 ttcgcgagca gctgcgtctc gaccttgcgg cggatggtca catcgtgcac cgccaccgcc
      961 agtagttggc ggccttccac ctcgatgcga tagcaggtgg cttcgacgtt gcgctccgag
     1021 ccgtccgcac aacgctggcg catctcgagc ggtgcgcgcg cggtggcatc ctggagcctg
     1081 agcaagaggt cgcggccttc gacgcctgcg acgctgaaga tgtcgccgaa ggtcagctcc
     1141 cgcagctctt cgagcgtgta gccgaggttg cgcagggagg cggggttccc cgctacccaa
     1201 cgcatggtgg cgggatcgag cgcgatggca gtcgtcggcc actgctcgaa caccgccagg
     1261 tgttgtgcgt cgtaaggcga tgcgggccgc cgaaaccgta gccaccacag cagtgcgcag
     1321 ccgatcgctg cgcaagcgag cccggccagc accccgatcg caaatgccaa ctggttcaca
     1381 ctttgtcccg ccaatgtctc aggaagtgtg gcggaaccgc cccgggggtg ccgtgcgcca
     1441 tgcacagttt gagtcgcccg tttggcgacg aaagcgacac acgcctcagg cagagtcgaa
     1501 actcacctcg catactgcgc cgcgtacgat ctcgccccgc tccgctgaga cgagcacggc
     1561 gttctcgccg aaggtgacgc cgtgcagctt cttgtcgaag cgaaactggc gcagcactgg
     1621 cgcgggatcg gtcgatggct caccggtctg ctgatcgatg gacggtatcg tgcagcggct
     1681 gcagggcttc acgagtcgca aggtgacccc atcgacggtg agtgcatcga tccggtcctc
     1741 cgcccaggcc ggcaagcctt cgagcacgac gttcggccga aagcgctcca tcggaatgcg
     1801 ctgcggcaga cgcgtgttga ggtcctcgag cgacgcctgg ttgcacacca gcaccgggaa
     1861 accatccgca aaacccaccg gggcgggagc agtgccggcg aaggccggat tggcaaatcg
     1921 ctcgacccgt gccggtacgc gcacgagtcg cgcggcttca cctgcgatat ggctcgccca
     1981 ctgcgccgcg gcctctcctt catcgagccc cacgcacata tcgcggtgca ctcgcacgga
     2041 ccggcgctcg ccggtggcaa cacggggcag ccgtaagcgc ggcacacccg gcgcctcgag
     2101 caacagctca ccggcatcga gctgtggaac gatgcaggcg agctgcgggt gcgtgcgctg
     2161 gctgagaaaa acgccgtttg cattgatcag catccactgc cgatcccatt cgaatcccgt
     2221 cgcgctcatg cgcacccgcg cgcagctcac tgcgcgtccg gacttgagcg ggtacacgaa
     2281 aagaccggaa atggttgcgc tggcgaccgt ggatcgctgc atctgtggcc agaccttgtg
     2341 cgtgcggctc gaggcagtct gccatatgct gcagcactcg ccacctgcga ggatcggcat
     2401 gcgcgcgaaa aagacctcca gcagagccac tacgggcatg aagggactgg tgcgcacggc
     2461 ggtaacggcc atggtgagcg ccagtgttat tttcggagga ccggcggcgc acagccgcac
     2521 gcagaaaccg ccgctctacg gccgccattg gatggcggtt accggcaaac cgatcgctgc
     2581 cactgccggg gcgaaagtct tcgcgcaggg aggcaacgcg gtggacgccg cctgcgccat
     2641 gctcgccgcc gccgcgacgg cctggaccac gctctcctgg ggcggagaga cacaggcact
     2701 catctataac ccgaacaccg gcagggtcat tgccatcaac ggcctcggcg ttgcccccac
     2761 gggcgcgacg cccgactact ttcacaagct cggtatgcgg tatccgccgg agtacggacc
     2821 gctcgccgcg gtgacaccgg gcacgcccgg aggcctcatg accatgctcg ccgagttcgg
     2881 cacgatgagc ctcgcgcagg tgctcgcacc cgccatcgag atggcggatg gcttccccat
     2941 ggatgaagcg cttgcgggca ccatcgagca cttcaaggac aagctcaagg agtggccgga
     3001 ctccaagcgt gtgatgcttc cgcatctcgg cagcagccac gagggaccga tgccgggaga
     3061 gatcttccgt cagccggatc tggcggtgac gctgcgcaag ctggtcgagg ccgagaagca
     3121 ggcgctcaag tccggcaagg accgcaaggc cgccatctac gcggcctacg agcgcttcta
     3181 ccgcggtgac attgcaagtg atctcgtgca ggccgtgcgc gcggcgggtg gtctgttcac
     3241 gctcgaggat ctcgctcact ggaaggtgca catcgaggag ccggtgcaca gcagctacaa
     3301 gggcatcgag gtctacaagc tcaacgcctg ggtgcagggt ccggtcatgc tgcagactct
     3361 caacatcctc gagaacttcg acctcaggtc catgggc
//
LOCUS       DATDMP010000499         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_524881, whole genome shotgun sequence.
ACCESSION   DATDMP010000499 DATDMP010000000
VERSION     DATDMP010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524881"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VI195_08740"
     CDS             <1..687
                     /locus_tag="VI195_08740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6824514.1"
                     /translation="EGMSLSLWARDATALADTVAACGAERALACAVDVTDSTQIEGAM
                     GSARARFGPVQVLVNNAGQAASAKFTDTGPELWHRIINVNLTGTYLCTRAAVPDMLQQ
                     GFGRIVNIASIAGLRGGAYLSAYVSSKHAVVGLTRALALEFAARNITVNAVCPGYVDT
                     DIVKNAVANISRKTGRSPAEALATLVATNPQGRLVSTAEVANAVVWLCRPGSESVTGQ
                     SLVLAGGEVT"
     gene            698..1210
                     /locus_tag="VI195_08745"
     CDS             698..1210
                     /locus_tag="VI195_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY6824515.1"
                     /translation="MPQEKARLKLVDTPMDAETRIREDHHLSVRLWLRMLSCTNRIEA
                     VVRQGLQAKFQTTLPRFDLMAQLERSRQGLRMSELSQRLMVTGGNVTGITDALEKEGL
                     VVREVDQSDRRAYRVRLTAEGQRQFRSMAAEHEQWVIALFDGMSQRSKQQLVELLGEL
                     KQHISQATSD"
     gene            1223..2032
                     /locus_tag="VI195_08750"
     CDS             1223..2032
                     /locus_tag="VI195_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY6824516.1"
                     /translation="MGTFSPQPQHFKWQLTGRVGLITLDRPERKNPLTFESYAELRDL
                     FRALVDVSDVRALILTGAGGNFCSGGDVHEIIGPLTQLDTAGLLRFTQMTGDLVKAMR
                     HCPQPIVAAVDGVAAGAGAILAMAADFRIGTPSAKTAFLFTRVGLAGCDMGACAMLPR
                     LIGQGRAAELLYTGRSMSAEEGAAWGFFNRLTSAEELPGAALALAQEIAAGPAFAHAM
                     TKKMMHAEWNMGIDQAIDAEAVAQAECMRTEDFRRAYRAFVAKQKPQFEGN"
     gene            2034..>2311
                     /locus_tag="VI195_08755"
     CDS             2034..>2311
                     /locus_tag="VI195_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824517.1"
                     /translation="MTLAPARGFLDWPFFEARHRELATRIQDWAATELAGSPHPESQA
                     AVDARCRELVGMLGAAGLASFAVPQTHGGGANAFDVRALALIRETLAW"
BASE COUNT          413 a          748 c          776 g          374 t
ORIGIN      
        1 gaaggtatgt ctctctcgct gtgggcacgt gatgcaaccg cgctcgcgga tacggtggcg
       61 gcatgcggtg cggagcgcgc cctggcgtgc gcagtcgatg tcacggattc gacccagata
      121 gaaggagcca tgggctccgc gcgcgcgcgc ttcggtccgg tgcaggtgct cgtcaataac
      181 gccggccagg cggcgagcgc caagttcacc gacaccgggc cggaactgtg gcaccggatc
      241 atcaacgtga atctcaccgg cacctacttg tgcacgcgcg ccgccgtgcc cgacatgctg
      301 cagcaaggct tcgggcgcat cgtgaacatc gccagcatcg cgggtctgcg cgggggcgca
      361 tacctcagcg cctacgtaag ctccaagcac gccgtggtgg gactcacgcg cgccctcgcg
      421 ctcgaattcg ccgcgcgcaa tatcaccgtg aacgcggtct gccccggcta tgtggatacc
      481 gacatcgtca agaatgctgt cgccaacatc tcacgcaaga ccggccgcag cccggctgaa
      541 gcactggcca cgctcgtcgc cactaaccca caagggcgac tggtgagcac tgcggaagtg
      601 gcgaacgcgg tggtgtggct gtgccgtccg ggcagtgaga gcgttaccgg ccaaagtctc
      661 gtgctggccg gcggcgaagt gacctgaaga gcaatccatg ccgcaagaga aggcgcgtct
      721 gaagctggtc gacaccccga tggatgccga aacccgcatc cgcgaagacc accatctctc
      781 ggtgcgcttg tggctacgga tgctcagctg caccaatcgt atcgaggcgg tcgtacgcca
      841 gggcctgcag gccaaattcc agaccacgct gccgcggttt gacctgatgg cacagctgga
      901 gcgttcacgg cagggtcttc ggatgagcga gctgtcgcaa cgcctgatgg tgaccggcgg
      961 caacgtcacg ggcatcaccg acgcactcga gaaggagggg ttggtcgtgc gtgaggtcga
     1021 ccaaagcgac cggcgcgctt accgcgtgcg cctgaccgcg gagggccagc gccagttccg
     1081 cagcatggcg gccgagcacg agcagtgggt gatcgcgctg ttcgatggca tgagccagcg
     1141 cagtaagcaa cagctggtcg agctgctcgg cgaactcaag cagcacatca gccaggcgac
     1201 gagcgactga aacggagtct gcatgggcac attttcacct cagccgcagc acttcaagtg
     1261 gcagctcacc ggccgcgtgg gtctgatcac cctcgaccgc ccggaacgca agaatccgct
     1321 cacgttcgag tcctacgccg agctgcgtga tctcttccgc gcgctcgtcg atgtcagcga
     1381 tgtgcgtgcc ctgattctca ccggtgccgg aggtaacttc tgctccggcg gagacgtgca
     1441 cgagatcatc ggtcccctga cccagctcga caccgcgggt ctgttgcgct tcacgcagat
     1501 gacgggcgac ctggtcaaag cgatgcgcca ctgtccgcag cccatcgtgg ccgctgtcga
     1561 cggtgtagcc gccggggccg gcgcgattct cgccatggcg gccgacttcc gcatcgggac
     1621 gccgagtgcg aagacggcgt ttctcttcac gcgcgtcgga ctggcaggct gtgacatggg
     1681 cgcctgcgca atgctgccgc ggctcatcgg gcagggccgc gccgcggaac tgctctacac
     1741 cggacgatcc atgtcggccg aggaaggcgc cgcctgggga ttcttcaacc gcctgacgag
     1801 tgccgaggag ctgcccggtg cggcgctcgc gctggcacaa gaaatcgccg ccggcccggc
     1861 gttcgctcat gccatgacca agaagatgat gcatgccgag tggaatatgg ggatcgatca
     1921 agcgatcgat gccgaagccg tggcgcaggc agagtgcatg cgaacggaag acttccggcg
     1981 cgcgtatcgc gcgttcgtcg cgaaacagaa gccgcagttc gagggcaatt gacgtgacac
     2041 tcgctccagc acgcggcttc ctggactggc catttttcga ggcgcggcac cgcgagctcg
     2101 cgacacgtat tcaggattgg gcggcgactg agctcgcggg ctcgccgcac cccgaatcgc
     2161 aagccgcggt cgatgcgcgc tgccgtgagc tcgtcggcat gctgggcgcc gcgggcttgg
     2221 cgagcttcgc ggtcccgcag acacacggcg ggggggcgaa cgcattcgac gtgcgcgctt
     2281 tggcgctgat ccgtgagacg ctcgcctggc a
//
LOCUS       DATDMP010000500         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1857846, whole genome shotgun sequence.
ACCESSION   DATDMP010000500 DATDMP010000000
VERSION     DATDMP010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857846"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VI195_08760"
     CDS             complement(<1..1195)
                     /locus_tag="VI195_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824518.1"
                     /translation="MVSTQQALRTELTAVSAPDFLAGGGEVGARLRGLDWSATPIGPP
                     ERWPQSLKTIVRVMLDSRYAMWMLWGPDLTFFCNDAYLPTVGIKRDWVLGARADVVWE
                     EIWPDIGPRISQVLEHGQATWDEGLLLFLERSGFTEETYHTFSYSPVYDDDSHVAGML
                     CVVTEVTERVIGERHLRVLRDLAARASGMETVQQACDRLVAVLAANLLDVPFSCLYVL
                     DEASPGGRLASHHGNLPARLRPAAPTPGDDPWQFAAAPGNDSRVVSLYDFADTIPSPL
                     WPEQPLTQAVLLPIQGQGSAAPIALLIAGVSPRRALDDSYLGFLDLIARQFAAAIADA
                     QAYEAERRRAEALAAIDRAKTIFFSNVSHEFRTPLTLMLGPIAEAVAHPATPDAVRAQ
                     LDLAHR"
     gene            1378..1722
                     /locus_tag="VI195_08765"
     CDS             1378..1722
                     /locus_tag="VI195_08765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY6824519.1"
                     /translation="MTYGNYAKALQCLSDPTRRQVFERLRAGPRSVGALARGLPVSRP
                     AVSQHLKALKGAGLVTHRSEGTRRVYYIDPQGLGELRRWLDGFWTDALESFRNEIAAT
                     HAAGRKRHEPHD"
     gene            1706..2194
                     /locus_tag="VI195_08770"
     CDS             1706..2194
                     /locus_tag="VI195_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6824520.1"
                     /translation="MNRTIEIAPVRKSVVVEASPSQAFAVFTAGIDRWWPKSHGIGAT
                     PITASFIEPHVGGRWYTRHEDGSDVVIGHVRVWQPAERLVVSWEISAEWKPDARPAVS
                     SEVEVRFSAEPGGGTRVDLEHRNFERMGAAAGEKMRKDVDGGWPHLLDLYAKHAVREE
                     KK"
     gene            2194..2880
                     /locus_tag="VI195_08775"
     CDS             2194..2880
                     /locus_tag="VI195_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6824521.1"
                     /translation="MSEYLVHLIPGSPFSRAVLATLEEKHAPYRVAAVVPGTLRAPQH
                     LSRHPFGRVPVLEHGEFRLYETQAILRYLDRVLPEPPLTPADPRRAARRDQAMNVNDW
                     YLVSGAGAVIGFQRIVGPRLMGLTPDESAIAAALPKARTVFEELAQLLGAQPYFGGES
                     VTLADLMLAPQLDFLQQTPEWEALSSKHGNLRHWLTRMQSRPSLLATSWEKVAARASA
                     GAAAVRAAAG"
BASE COUNT          490 a          974 c         1024 g          449 t
ORIGIN      
        1 tgcggtgtgc gagatcgagc tgcgcgcgca cggcatccgg cgttgccgga tgcgcgaccg
       61 cctccgcgat cggcccgagc atgagtgtga gcggggtgcg gaactcgtgg ctgacgttgg
      121 agaagaaaat cgtcttggcg cgatcgatgg ccgcgagggc ctcggcccgc cgccgctcgg
      181 cctcgtaagc ctgtgcatcg gcgatcgcag cggcgaactg ccgggcgatg agatcgagaa
      241 agccgagata ggaatcatcc agcgcccggc gcggagagac cccggcgatg agcagggcta
      301 tcggcgccgc cgaaccctgt ccctggatgg gcagcagcac cgcctgtgtc agcggctgct
      361 ccggccagag cggagaagga atcgtgtcgg caaagtcgta cagcgagacg acgcgcgagt
      421 cgttgccggg cgcggcggcg aattgccagg gatcatcgcc cggcgtcggc gcggcaggcc
      481 gcagccgcgc gggaaggttg ccgtggtggc tggccagcct tcccccaggc gatgcctcat
      541 cgagcacgta cagacaggaa aaaggcacat cgagcaggtt cgcggcgagc accgcgacca
      601 ggcgatcgca ggcctgctgc acggtctcca tgcccgaggc tcgcgcggcg aggtcgcgca
      661 gcacgcgcag gtgccgctcg ccgatcaccc gctcggtaac ctcggtgacc acgcacagca
      721 tgccggcaac gtggctgtca tcgtcataca ccgggctgta ggagaaggtg tgataggtct
      781 cctcggtgaa gccgctgcgc tcgagaaaca gcagcagccc ctcgtcccag gtggcctgcc
      841 cgtgctcgag cacctggctg atgcgcggcc cgatatccgg ccagatctcc tcccatacca
      901 cgtcggcgcg ggcgccgagc acccagtcgc gcttgatgcc caccgtaggc aggtaggcat
      961 cgttgcagaa gaaggtgaga tcgggccccc acagcatcca catggcgtag cgcgagtcca
     1021 acatgactcg cacgatggtc ttgagcgatt gcggccagcg ctcgggcggc ccaatcgggg
     1081 tggcgctcca gtcgagcccg cgcaggcggg ctccgacctc gccgccgccg gcaaggaaat
     1141 cgggggcgga aacggcggtg agctccgtgc gcagagcctg ctgcgttgag accaagaggt
     1201 ctccctgccg atagaggagg agcggcgagt gtacccggac gagcgtttcc gccctgtcgg
     1261 cgaaagccgc attgcgcggg tcacggccga gcccccttcg ccggctccgt cacaggccag
     1321 gcctcgcgct ttcagaggct tgcttgactt ttggttagta tccacttacc gtaagttgtg
     1381 acttacggaa attacgccaa ggcgctgcag tgtctgagtg acccgacccg ccgccaggta
     1441 ttcgagcggc tgcgcgccgg cccgcgctcg gtaggcgccc tggcccgtgg cttgccggtc
     1501 agccgtcccg cggtgtcaca acacctcaag gcactgaagg gtgccggact cgtgacgcat
     1561 cgatccgaag gtacgcgccg cgtctactac atcgatccgc aggggctcgg tgagttgcgg
     1621 cgctggctcg atgggttttg gaccgacgcg ctggagtctt tcaggaacga gattgcggcg
     1681 acccacgccg cgggaaggaa gagacatgaa ccgcacgatt gagatcgccc ccgtccgtaa
     1741 atccgtggtg gtcgaggcga gcccatcgca ggcgtttgcc gtcttcaccg cgggtatcga
     1801 tcgctggtgg ccgaagagcc atggcatcgg tgccacgccc atcaccgcct cgttcatcga
     1861 gccccacgtc ggtggccgct ggtacacgcg ccatgaggac gggagcgatg tggtcatcgg
     1921 acacgtgcgc gtctggcaac cggccgagcg gctggtcgtc tcctgggaga tcagtgccga
     1981 atggaagccg gacgcgcgac cggctgtcag ctccgaggtc gaggtccgct tcagcgccga
     2041 gccaggcggg ggtacccgtg tcgatctcga gcatcgcaac ttcgagcgta tgggcgccgc
     2101 ggccggcgag aagatgcgca aggacgtcga cggcggctgg ccccacctgc tggatctcta
     2161 tgcgaagcac gccgtgcgcg aggagaagaa gtgatgagcg aatacctggt tcacctgatt
     2221 cccggcagtc ccttctcgcg cgccgtgctg gcgacgctcg aggaaaagca tgcaccctat
     2281 cgagtcgcgg ccgtagtgcc gggcacgctg cgcgcgccgc agcacctgag ccgccacccc
     2341 ttcggccgcg tgccggtgct cgagcacggg gagttccgcc tgtatgagac gcaggcgatc
     2401 ctgcgctatc tcgatcgggt gctgccggaa ccgccgctca caccggcaga cccgcgccgc
     2461 gcggcgcgca gggaccaggc gatgaacgtc aacgactggt atctggtctc gggcgccggc
     2521 gccgtcatcg gcttccaacg catcgtcgga ccgcggctca tgggcctcac ccccgatgag
     2581 agtgcgatcg ctgccgcgtt gccgaaggcg cgcacggtgt tcgaagagct ggcgcagctg
     2641 ctcggcgcac agccctactt cggcggcgag agcgtgacac tcgccgacct catgctcgct
     2701 ccgcagctgg atttcctgca gcagacgccg gagtgggaag cgctcagctc caagcacggc
     2761 aacctgcggc actggctcac gcgcatgcag tcgcgcccga gcctgctcgc caccagctgg
     2821 gagaaagtcg ccgcgcgcgc cagcgcggga gcggctgcgg tgcgcgccgc cgccggctag
     2881 ctcagcgcgc gccggtaccg gtcggcgcga tgggcggcgg tgattcgaac tgttgct
//
LOCUS       DATDMP010000501         2863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_81215, whole genome shotgun sequence.
ACCESSION   DATDMP010000501 DATDMP010000000
VERSION     DATDMP010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81215"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..732
                     /locus_tag="VI195_08780"
     CDS             64..732
                     /locus_tag="VI195_08780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6824522.1"
                     /translation="MSGGHDKLDAQHEQWQSTFRGNPDMYGTNPSEPGAYAADLFSRE
                     QIRDLLELGAGQGRDTLTFLRAGLTVTALDYATSALTRLQQAATDAGLADQLTTVAHD
                     VRQPLPLPDSGFDAAYSHMLFNMALTTTELETLSGEVHQALRPAGLHVYTVRHTGDAH
                     YGTGISHGDSMFENGGFIVHFFDRALVDRLATGFTLLDLSAFEEGSLPRRLWRITLRR
                     EGAP"
     gene            complement(1142..2716)
                     /locus_tag="VI195_08785"
     CDS             complement(1142..2716)
                     /locus_tag="VI195_08785"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEY6824523.1"
                     /translation="MSSGTTRGLRLAELSDEERNERFDRLQERMRGVWEIMRRNEEGE
                     SVVVIPSVSVDRVGERSGSLQQAYEERFLVLLLLLRHPRLRMIYVTSMPIAPKIVEYY
                     LALLTGVIPSHARARLSLISVGDASPRPLSEKLLERPRLLSLIRSLVPNPLRSHLVPY
                     NTTPLERDLAIALGIPMYAADPRFFPLGTKSGCRKLFADLAVRHPLGFENLRSIEDVI
                     DALTRLRAARPDVTEAMVKLNEGVSGEGNALVDLHNLTVSGSPGEREETARRVQEMAF
                     ELPGTPFSSYAAKLAERGGIVEERITGLELRSPSVQLRVTPFGEVELLSTHDQMLGGP
                     SGQSYLGCTFPADFEYARMISADAQRIGERLAHEGVLGRFAIDFVVVRSGEGSWTTYA
                     IELNLRKGGTTHPFLTLQFLTDGRYDPATALFLTPNNREKHLVATDHLESPMLRGLAV
                     DDLFDIVARHGLHFDPARQQGVVFHMISCLTELGRVGLTAVGDTPASAKAMYQKAERI
                     LFEEARESLAERPLLE"
BASE COUNT          525 a          943 c          902 g          493 t
ORIGIN      
        1 aaatccggcg cccccgcctg aggaggcgct gcgctgatgg ttccgcccgg cgggcggctc
       61 cccatgagtg gtggccatga caagctcgac gcccagcacg agcagtggca gtccacattt
      121 cggggaaacc cggacatgta tggcaccaac ccgagcgagc caggggctta cgcggccgac
      181 ctgttctccc gcgagcagat ccgcgacctg ctcgagctcg gcgccggcca gggccgcgac
      241 accctcacgt tcctgcgcgc cggactgacc gtgactgcgc tggactacgc caccagcgcc
      301 ctcacccgcc tgcagcaggc cgccaccgac gccgggctgg ccgatcagct gaccacagtc
      361 gcacacgacg tccgtcaacc gctgcccctg cccgacagcg gtttcgatgc cgcgtacagc
      421 cacatgctgt tcaacatggc gctgaccacc accgagctgg aaacgctcag cggcgaggtg
      481 caccaagcac tgcgcccggc aggactgcac gtctacaccg tccggcacac cggcgatgcg
      541 cattacggca caggcatcag ccatggcgac agcatgttcg agaacggcgg gttcatcgtc
      601 cacttcttcg accgcgccct ggtcgaccgg ctcgccaccg ggttcaccct gctcgacctg
      661 agcgctttcg aggaaggcag cttgccacgc cgactgtggc ggatcacgct gcgccgcgag
      721 ggcgcaccct gagcaccgcc cctcgttcct cgcggcacag gcgctgccaa agcgcggcgc
      781 acaatggcaa ccggggcgcg cgattacggt tagctcaggt actgctgccg cggccattcg
      841 gcacgaccgc gtcccggcat aacgcggcgt gtcaagtcgt tagcggctga ctcgtcggcc
      901 aacagtgctt cgcggcgttt ccacgcacac ctcgagcaat tgcgtgatcg gtcccgcaaa
      961 acggtttgtt cgcgcggccg ctgcaagctc atcaggtgat caccggccga ggcgcccgga
     1021 caaatccgtg accgtcctgc ggcggcgcat gaggcgcgct gccgccgtgg atgagcagca
     1081 aggctgcggg agaacctccc tgtagtcatg atcgtccgcc cggttacagc tcccggtggg
     1141 tctactcgag cagtggccgc tcggccagag attcgcgggc ctcttcgaac aagatacgtt
     1201 cagctttttg atacatggcc ttggctgacg cgggcgtgtc cccaactgca gtgaggccta
     1261 cacgcccgag ctccgtcagg cagctgatca tatggaacac gacgccctgc tgcctggccg
     1321 ggtcgaaatg cagaccgtgt cgcgccacga tgtcgaatag gtcgtctacg gctagtccgc
     1381 ggagcatagg cgattcgaga tgatctgtcg caacgaggtg cttctcccgg ttgtttgggg
     1441 taaggaacag tgccgttgcc gggtcgtacc gaccgtcagt gaggaattgc agggtgagga
     1501 agggatgagt cgttccacct ttccgaagat tcagctcaat cgcataggtc gtccaggagc
     1561 cctcgcccga ccgcacaacg acaaagtcga tcgcaaatcg gccaagcaca ccttcatggg
     1621 ccagtcgttc gccgatccgc tgcgcatccg cgctgatcat cctggcgtac tcgaaatctg
     1681 cgggaaacgt gcaccccaag tagctctgcc cgctagggcc gcccagcatt tggtcgtggg
     1741 tcgaaaggag ctctacttct ccgaagggcg tgactcgcag ttgaacgctc ggactgcgca
     1801 actctaggcc agtgatgcgc tcctcgacga tgccaccccg ctcggcgagc tttgcggcat
     1861 aggagctgaa aggagtgcct ggcagctcga acgccatttc ctgcacgcga cgtgccgttt
     1921 cctccctttc acccggcgac ccggaaacgg tcaggttgtg caggtcaacc agcgcgttgc
     1981 cctcgccgga cacgccttcg ttaagtttaa ccattgcttc ggtgacatca ggccgcgctg
     2041 cccgcagacg tgtcagcgcg tcgataacat cctcgataga gcgcagattc tcgaagccca
     2101 gcgggtgtct gaccgccagg tcggcgaaga gcttccggca cccggacttg gtccccaggg
     2161 gaaagaaacg ggggtcggcg gcgtacatcg ggatgcccag cgcgatggcg aggtcccgct
     2221 cgagtggagt ggtgttgtac ggcaccaggt gcgaccgcaa ggggttcggc acgagtgagc
     2281 ggatcagcga cagcaggcgc gggcgctcta ggagcttttc actgagcggc cggggcgatg
     2341 cgtcgccaac cgagatgagg gacagtcgcg cgcgggcgtg gctaggtatc acacctgtga
     2401 gcaacgcgag gtagtactcc acgatcttgg gggcgatcgg catggaggtc acatagatca
     2461 tgcgcagtcg agggtgcctc aagagcagca agaggacgag gaaccgctcc tcgtacgctt
     2521 gctgtaggga gccacttcgc tcgccaacgc ggtccacgct gaccgaggga atgacaacga
     2581 cggactcgcc ctcctcgttg cgtcgcatga tctcccagac gccgcgcatt ctctcttgga
     2641 ggcggtcgaa gcgttcgttg cgttcctcgt cgctgagttc tgccagcctt agccctcgcg
     2701 tcgtcccgga actcatgagg tgacccttgc agagtcgagt ccagaaacct cgaaggtgcg
     2761 gatcggccgg gagaagccac gcaacgatag ctcgccgatc agccggctca ccacgagctc
     2821 ctcggctgcc gcgttcacgc gctgggtaat gaggatctgc cag
//
LOCUS       DATDMP010000502         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_858550, whole genome shotgun sequence.
ACCESSION   DATDMP010000502 DATDMP010000000
VERSION     DATDMP010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858550"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VI195_08790"
     CDS             complement(<1..186)
                     /locus_tag="VI195_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6824524.1"
                     /translation="MAPAVNGVLRPHVLVVDDEESLRRLVDEYLVENDFRVTAVVNGA
                     GLLSTLGSEVIDLVLLDL"
     gene            189..539
                     /locus_tag="VI195_08795"
     CDS             189..539
                     /locus_tag="VI195_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6824525.1"
                     /translation="MKRVLIVEHDRLHRELIGEWLAAAGVQALWPETATQAVNDIDAI
                     LVDVACQQQAHELLLPWRRAYPRAAIVLSSGRFSRGDMANDAMALRLGATRILAKPFT
                     RTDLWAALDLSPAH"
     gene            506..>2519
                     /locus_tag="VI195_08800"
     CDS             506..>2519
                     /locus_tag="VI195_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824526.1"
                     /translation="MGSSRSEPGSLTLATTVDAARREGAGRHSLRLRLVLIVVAVCGA
                     VSLSTLFDMLQERRETLALAERQHDNIAGALAEQAARALQATDLILRQAMLLDPDTPG
                     SPDRESIPDLLSRHMSGVPQVRNLFLFDPARQLHLSTALTGAKSDLSDRSYYLAQRAQ
                     PDLGLFVSEPFISRATGEPTFVLSRRLPGPGFRGIAGAAVDVIYIRRLYQALDLGPGS
                     SIELLRADGRTLVSRERPEVTPQPSPWLPALQTLGTAAALHTTVEHPQLGRTQVSLRR
                     VPGYPAVVLVSRGERDILAVWRSKAWKNVARTLIISLLAAVLLIAFLRQLERHERVTA
                     QLHQSQKLEALGTLAGGIAHDFNNVLGAVLGYAELAGQQSAPDSAQRRYIDNIVVAAN
                     RARELVARILAFSRPGVGSARPVLLQKILSEVHSLTCAALPPSVTVAMEVAPAPLVVA
                     GDGSQLHQMLANLLTNAVQAVGSAGEVRVRAGAVEVIEARDCTVGRLQSGRYARIDVS
                     DTGPGMSAEQVERIFEPFFTTKPVGAGTGLGLSLVHGIVLDHSAALEVSSHAGVGTTF
                     SVYLPLTKEEPAQEPAPLAAPSGNGETILVVDDEPSLVALAEEVLASLGYEPVGCVGA
                     QQALRVFRGAPQRFDAVLTDAIMPEMSGLELLAEVRRVRPELPVVLV"
BASE COUNT          401 a          875 c          830 g          413 t
ORIGIN      
        1 cagatcgagc agcaccaggt cgatgacttc gctgccgagc gtactcagca accctgcgcc
       61 gttcaccact gcggtcacac ggaaatcgtt ctccaccaga tactcatcaa cgagccgtcg
      121 cagcgactcc tcgtcgtcca ccaccagtac gtgcggccgc agcaccccgt tcaccgcggg
      181 agccaaccgt gaagcgcgtg ctgatcgtgg agcatgatag gctgcatcgc gagctgatcg
      241 gcgaatggct cgccgctgcg ggcgtgcagg cgctctggcc cgagaccgcg acgcaggcgg
      301 tgaacgatat cgatgcgata ctggtcgacg tggcatgtca acagcaggct catgagctcc
      361 tcttgccctg gcgccgcgcc tatccgcggg cggccatcgt gctgtcctcg ggacgctttt
      421 cccggggaga catggcaaat gatgccatgg cgctgcgact tggagcgacg cgcatcctgg
      481 cgaagccctt cacacgcact gatctatggg cagctctcga tctgagcccg gctcactgac
      541 acttgccacc acggtggatg ccgcacgccg cgaaggcgcc ggccgccact ccctacgcct
      601 gcgcctggtg ctcatcgtcg tggccgtatg tggcgcggta tcgctgtcga ccctgttcga
      661 catgctgcag gagcgccggg agacgctcgc acttgccgag cgccagcacg acaacatcgc
      721 gggcgctctc gccgagcagg cggcgcgtgc cctgcaggcc accgacctga tcctgcggca
      781 ggcgatgctg ctcgaccccg ataccccagg ctcgcccgac cgcgaaagca ttccggatct
      841 gctgagccgg cacatgagcg gggtgccgca ggtgcgtaac cttttcctgt tcgatcccgc
      901 acggcagctg cacctgagca ccgcgctcac cggcgcgaag agcgatctca gcgaccgctc
      961 gtactatctc gcccagcgcg cgcagccgga ccttggactg ttcgtcagcg agcccttcat
     1021 cagtcgggcg accggcgaac ccacgttcgt gctgagccgc cgcctgcccg ggccgggatt
     1081 ccgcggcatc gccggcgccg cagtggatgt catatatatt cgtcgcttgt accaggcgct
     1141 cgatctcggt cccggcagct ccatcgagct gctgcgcgcg gatggcagga cgctggtgag
     1201 ccgtgagcgc ccggaggtaa ccccgcagcc ctcgccctgg ctgcctgcgc tgcagaccct
     1261 cggcacagcc gcggcactgc acacgaccgt cgagcatccg caactcggcc gcacgcaggt
     1321 gagtctgcgc agggtgccag gatacccggc ggtggtgctc gtgagtcgcg gtgagcgcga
     1381 catcctcgcc gtgtggcgca gcaaggcgtg gaagaacgtt gcacgtacgc tgatcatcag
     1441 cctgctcgct gccgtgctgc tcattgcctt cctgcggcag ctcgagcggc acgagcgtgt
     1501 caccgcccaa ctgcaccagt cgcagaagct cgaggcgctc ggcacgctcg caggcgggat
     1561 cgcccacgat ttcaacaatg tgctgggcgc cgtgctcggc tacgcggagc ttgccggtca
     1621 gcagagcgca ccggacagcg cccagcggcg ctacatcgac aacatcgtcg tggcggcgaa
     1681 ccgcgcgcgc gagctagtgg cgcgcattct cgccttcagc cgccccggtg tcggcagcgc
     1741 acggccggtc ttgctgcaga agatcctgag cgaagtgcat agcctcacct gcgcggcgct
     1801 tcctccttcg gtcacggtcg caatggaggt agcgcccgcg ccgctcgtag tcgccggcga
     1861 tggctcgcag ctgcatcaga tgctcgccaa tctcctcacc aacgcggtgc aggcggtggg
     1921 ctcggccggg gaggtgcgcg tacgcgccgg cgccgtggag gtgatcgaag cacgcgactg
     1981 cacggttgga cggttgcagt ccggccgcta cgcccgcatc gatgtgagcg atacgggacc
     2041 tggcatgagc gcggagcagg tcgagcgcat cttcgagccg ttcttcacta ccaagccggt
     2101 cggtgcgggc acggggttag gcctgtcgct ggtgcacggg atcgtgctcg accacagtgc
     2161 agccctcgag gtgagcagcc acgccggcgt cggtaccacc ttcagcgtgt acctgccgct
     2221 caccaaggaa gagccggcgc aggagccggc accgcttgca gcgcccagcg gcaacgggga
     2281 aaccatcctg gtcgtcgatg acgagccgag cctggtcgcg ctcgccgaag aagtgctcgc
     2341 ctcgctcggc tacgaaccgg tcggttgcgt cggcgcgcag caggcgctgc gcgtatttcg
     2401 cggcgcgccg caacgcttcg atgcggtgct caccgatgcc atcatgcccg agatgtcagg
     2461 gctcgagctg cttgccgagg tgcgccgtgt gcggccggag ctacccgtcg tgctggtga
//
LOCUS       DATDMP010000503         2763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_414515, whole genome shotgun sequence.
ACCESSION   DATDMP010000503 DATDMP010000000
VERSION     DATDMP010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414515"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VI195_08805"
     CDS             complement(<1..401)
                     /locus_tag="VI195_08805"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY6824527.1"
                     /translation="MLIALACSGCATVQTTKPGAVGIERRQTMLVSAESVEQGAQKAY
                     AGEVQQASSSGKLNADPALTARVRHITDRLIPVTAAFRPDAPQWKWEVNTLTTPEINA
                     YAMPGGKIMVYSGLVEKLQLSDAEIAAVLGH"
     gene            complement(501..1385)
                     /locus_tag="VI195_08810"
     CDS             complement(501..1385)
                     /locus_tag="VI195_08810"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEY6824528.1"
                     /translation="MAAIAGVLLLLDGGWSEAQTTHDRAFWAALRAADFRLPQGAAVL
                     PLTLEAAALLGSTDAELRDDIAYGAIETWVYREQRLDAAELDQLRGTLAANARHGLGE
                     AAGDGLFLRSFSLLALAVLAAEDAKRPFLDAQKFDGLIELALDELARERDLRGYVPGK
                     GWGHATAHCADLLKFLARSHWLRAPQQSRIVEAIAERLRSAGLVFVWGEDARLAAALS
                     SIARRPDADPAPFVAWFQRLEAEHAALWSGSFDAARYVPVRAQLNSLNAFAADLDADS
                     GNGAAIRAAVRSLRAAAQ"
     gene            complement(1435..2541)
                     /locus_tag="VI195_08815"
     CDS             complement(1435..2541)
                     /locus_tag="VI195_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY6824529.1"
                     /translation="MRLRYKIVSVILIVLAIGVLSLAIAMSRNAPCGSSPPLAAGATP
                     MKAIVHRCYGSPAVIRYEDIPKPNPAEDEILVRVHAASLNPLDWHYLEGTPYMVRLDA
                     GLGTPANPRLGVDFSGTVEAVGKKVTRFKAGDEVFGGKFGALAEYVAVREDRAIALKP
                     ANLTHEQAAAVAIAAITALQGLRDEGHIHAAEQVLVNGASGGVGTFAVQLAKSFGATV
                     TGVCSTRNVELVRSIGADRVIDYTREDITKGPQRYDLILDNVATHSLFDYKRILNPNG
                     SYVMIGSTDPGRWFAWLWKPLQGWVISHFTSQKFVMILADLNKEDLATLAALMQSGKV
                     TPVVDKTYKLEDAAEAFSYLEKGHARGKVVVTVD"
BASE COUNT          458 a          910 c          911 g          484 t
ORIGIN      
        1 tcgtggccga gcactgccgc aatctccgcg tccgacagct gcagcttctc gacgagcccg
       61 ctgtacacca tgatcttgcc gcctggcatg gcgtaggcgt tgatctcggg cgtcgtgaga
      121 gtgttgacct cccacttcca ctgcggagcg tcggggcgaa aggccgcggt gaccggaatg
      181 agccgatcgg tgatgtgtcg tacgcgcgcg gtcagtgcag gatctgcgtt gagcttgccg
      241 ctcgagctcg cctgctgcac ctcgccggca taggctttct gcgcgccctg ctcgacggac
      301 tccgccgaga cgagcatggt ctgtcggcgc tcgatcccga cggcccccgg tttggtggtc
      361 tgcacggtcg cgcagccact gcaggccagc gcgatcagca agccccagat gcagcccctt
      421 tgcgctccac gaatatgcgg tgcgcgccgc gccattcccc gcctcactgc cgtcctttcg
      481 gttctcgggt gttcccgatt ttactgtgcc gcggcacgca gcgagcgcac ggcggcgcga
      541 atggctgcgc cattgccgga gtccgcatcg aggtccgcgg cgaatgcatt gagcgagttc
      601 aattgcgccc ggaccggcac ataacgggcg gcatcgaagc ttccgctcca cagcgcggcg
      661 tgctcggctt cgaggcgctg gaaccaggcc acgaacggtg ccggatccgc atcggggcgg
      721 cgtgcgatcg agctgagcgc ggcggcgagc cgggcgtcct cgccccaaac gaacacgaga
      781 ccggccgaac gcagccgctc agcgatcgcc tcgacgatgc gtgactgttg cggcgcacgc
      841 aaccagtgac tacgagcgag gaacttcagc aggtccgcgc aatgcgccgt cgcatgtccc
      901 cagcctttgc caggcacata gccgcgcagg tcgcgctcgc gcgcgagctc atcgagcgca
      961 agctcgatga gaccatcgaa cttctgcgca tcgagaaagg ggcgcttcgc gtcctcggcg
     1021 gcgagcaccg ccagcgccag cagcgagaag gagcgcagaa atagtccatc gcccgctgct
     1081 tcaccgaggc cgtggcgggc attcgcggcg agagtgccgc gcagttgatc gagctcagcc
     1141 gcatcgagcc gctgctcgcg gtagacccac gtctcgattg ccccataagc gatgtcatca
     1201 cgcagctcgg catcggtcga tccgagcagc gccgccgctt cgagcgtcag gggaagcacg
     1261 gctgcaccct gcggaagcct gaagtccgct gcgcgcagcg cagcccagaa cgctcgatcg
     1321 tgtgtcgtct gcgcctcgct ccagccacca tcgagcagca gcagcacacc tgcgatcgcg
     1381 gccacgagcc cgcgccgccg cgtgcggccc gcggatctgg tgttgaggtt cgagctaatc
     1441 aacggtcacg acgactttgc cgcgcgcatg ccctttttcc aggtagctga acgcctcggc
     1501 ggcgtcctcg agcttatagg tcttgtcgac caccggggtc acctttccgg actgcatcag
     1561 cgcagcgagt gtggcgaggt cctccttgtt gaggtccgca aggatcatga cgaatttctg
     1621 gctggtgaag tgcgagatca cccagccctg cagcggcttc cacagccagg cgaaccaacg
     1681 gccaggatcg gtcgagccga tcatgacgta gctgccgttg ggattcagga tgcgcttgta
     1741 atcgaacagc gagtgcgtcg cgacgttgtc cagaatcaga tcgtagcgtt gtgggccctt
     1801 ggtgatgtcc tcgcgtgtgt agtcgatcac ccgatcggca ccgatcgagc gcaccagctc
     1861 gacatttctc gtgctgcaca ctccggtgac ggtcgcgccg aatgacttgg cgagctgcac
     1921 tgcgaacgtg ccgacaccac ccgaggcgcc gttgacgagc acctgctcgg cggcgtgaat
     1981 gtgtccctcg tcgcgcagtc cctgcagcgc ggtgatggcc gcgatggcaa ctgccgccgc
     2041 ctgctcgtgg gtcaggttgg cgggcttgag ggcgatggcg cggtcctcgc gcacggcgac
     2101 atactcggcg agcgccccga acttgccgcc gaagacctca tccccggcct tgaagcgcgt
     2161 aaccttcttg cccactgcct cgaccgttcc ggaaaagtcg acgcccagcc gcgggttcgc
     2221 cggcgtgccg agaccggcat ccagacgcac catgtacggg gtgccctcga ggtagtgcca
     2281 gtcgagcggg ttgagcgagg ccgcgtgcac cctgacgagg atctcgtcct ccgcggggtt
     2341 gggcttcggt atgtcctcgt agcggatgac ggccggtgag ccgtaacaac ggtgcacgat
     2401 ggccttcatg ggcgtcgcac cggcggcgag tggcggtgag gacccgcagg gcgcgttgcg
     2461 actcatggcg atggcgagcg acagcacacc gattgccagc acgatgagga tgacgctcac
     2521 gattttgtag cgcagtctca tgactccccc ggcgctttct gccacgccgc gtgcgcgtgc
     2581 cgcgtgggtg gatcctagtg tgatgtcccg tatgtaactt ggcgcagcga gcgtggtcca
     2641 aggagtatcc ttggccagcg gagcgctcga tgggacgacc agcaaaagct ttgacggtga
     2701 gtacagaaga ccggttgcag cttgagtcga ttgcccgctc gcagagcctg cctgcgggaa
     2761 tcg
//
LOCUS       DATDMP010000504         3157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_303665, whole genome shotgun sequence.
ACCESSION   DATDMP010000504 DATDMP010000000
VERSION     DATDMP010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303665"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VI195_08820"
     CDS             <1..656
                     /locus_tag="VI195_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824530.1"
                     /translation="APNPSAPYISYANVTRVHLDDSVVWKYYDPKGNTGGGISKMAMS
                     FTLDLGCEYGGGVHCAHGPNKANFFGAMLYNRAWLDHDLFAVTLGGGFMNNPGRYLVL
                     LPPINGATAATGTPYFTENPGQKIYQWDLQLNLQYMPRDWITWWTEATFRHSDVPYWS
                     GPGGVTPPLGNTGSPASFVCNNGSVIGSDACAGEGGIWYPDLRTREVIWGAGVLVKF"
     gene            869..2023
                     /locus_tag="VI195_08825"
     CDS             869..2023
                     /locus_tag="VI195_08825"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY6824531.1"
                     /translation="MSAPSRQEAHARLVWANVLMFALTFAAAVALVPWYGLTHGFSTA
                     DWGVFAFFLVANGTAITAGYHRLWAHRTYEAHWSLRLWYLLFGTMALQNSVFAWCSGH
                     RRHHLHVDDEERDPYSARRGFWFSHIGWMLREYPSGRDDFSNIPDLKRDPLLQFQHRY
                     YVPLALLANFGLPLAAGLLFHDVWGMLILAGVLRLVWSHHVTFFINSLAHMWGSRPYT
                     EDNSARDNPVLAVVTYGEGYHNFHHIFAHDYRNGVRWWQWDPTKWLIASLQLVGLTRR
                     LKRTPFFQIQRALLALQFQRAQARLARLPAAGAAAHIESLRARLSHEYESFLAAIAEW
                     ARVKEQWLGEKKRAVREQWEQVQLQHCLREIERRLSLQLRRMRLLQAQLA"
     gene            2142..2864
                     /locus_tag="VI195_08830"
     CDS             2142..2864
                     /locus_tag="VI195_08830"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY6824532.1"
                     /translation="MGRIFEVRKHTMFARWNRMAKQFARIAKDITMAVKAGGPDPTAN
                     PTLRRVIHNARAVNMPKDKIEAAIKRASGRDATHYQQVLYEGYAPYGVALLVETATDN
                     PTRTVAAVRNIITKGGGNLATNGSVSFMFRKMGVFRLDPQGLDQDELELYLIDHGLEE
                     MGESTGEKGEPQLVIRCGFADFGRMQEALEARGIAALSAEHEYICTVPTELAEEKATE
                     VLTLIDRLEQDDDVQKVFHTLA"
     gene            2878..>3157
                     /locus_tag="VI195_08835"
     CDS             2878..>3157
                     /locus_tag="VI195_08835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6824533.1"
                     /translation="MPDHPHAYRLQPARAGDAALLAAMSAALIEQGLKPAWGAARIRW
                     HVRDADSVVLTARLGAAIAGFAIMRYGEDVAHLNLLAVAPAPRRRGVAR"
BASE COUNT          515 a         1098 c         1029 g          515 t
ORIGIN      
        1 gcgcgcccaa tccgtccgcg ccctacatca gctacgccaa cgtcacgcgc gtgcacctgg
       61 atgacagcgt ggtgtggaag tactacgacc ccaagggcaa taccggcggg ggtatctcga
      121 aaatggccat gtcgttcacg ctggacctcg gctgcgagta cggcggcgga gtgcactgcg
      181 cgcacgggcc caacaaggcg aacttcttcg gcgcgatgct ctacaaccgc gcgtggctcg
      241 atcacgacct gttcgccgtg acgctcggcg gcggcttcat gaacaatccc ggccgctacc
      301 tggtgctgct gccgccgatc aacggtgcca cggccgccac cggcacacca tacttcaccg
      361 agaatcccgg ccagaagatc taccagtggg acctgcagct caatctgcag tacatgccgc
      421 gtgactggat cacctggtgg accgaggcga ccttccgcca ctccgatgtg ccctactgga
      481 gcgggcccgg tggcgtgact ccgccgctcg gcaacaccgg ctcaccggcg agcttcgtct
      541 gcaacaacgg cagcgtgatc ggcagcgatg cctgcgcggg ggaaggtggc atctggtacc
      601 cggatctgcg gacccgcgag gtgatctggg gcgcgggcgt tctggtcaag ttctagggcg
      661 cacaaatggc cgcggggcgc ccggaagcac gtcacggtcg tgccctaggc cgctgaatcg
      721 agcacgcagc cgcggccagg gccttaaaaa cgccgacgca gaccccacat ccgccctcgc
      781 ggcatgctaa tttactgcgc cctcttccga gggccggcaa ggggcccccc gaggggcggc
      841 cgcagccatc ccgaggattc cgccgctcat gtccgccccg tcacgtcagg aagcgcacgc
      901 ccggctcgtc tgggccaatg tgctgatgtt cgcgttgacc ttcgccgccg ctgtggcgct
      961 cgtgccctgg tacggcttga cccacggttt cagcacggcc gactggggcg tgttcgcttt
     1021 cttcctggtc gctaacggta cggccatcac ggccggctac catcgcctct gggcgcaccg
     1081 cacttacgag gcgcactgga gcctgcggct ctggtatctg ctgttcggca cgatggcgct
     1141 gcagaacagt gtgttcgcct ggtgcagtgg ccaccgcagg catcacctgc acgtcgatga
     1201 cgaggagcgc gacccgtatt cggcgcggcg cgggttctgg ttctcgcaca tcggctggat
     1261 gctgcgtgag tatccgagcg ggcgggacga cttcagcaac atcccggatc tgaagcgcga
     1321 tccgctgctg cagttccagc accgttatta cgtgccgctg gcgctgcttg ccaacttcgg
     1381 tctgccgctc gccgcgggac tgctgttcca cgacgtatgg ggaatgttga ttcttgccgg
     1441 cgtgctgcgc ctggtgtgga gccaccatgt caccttcttc atcaactccc tggctcacat
     1501 gtggggcagc cgcccgtaca ccgaagataa ttccgcgcgc gacaacccgg tgctggcggt
     1561 ggtcacctac ggcgagggct atcacaactt ccaccatatc ttcgcgcacg actatcgcaa
     1621 cggcgtgcgc tggtggcagt gggaccccac caagtggctg atcgcctcgc tgcagctcgt
     1681 tggcctgacg cgccgcctca agcgcacccc gtttttccag atccagcgcg cgctgctcgc
     1741 gctgcagttc caacgcgcgc aggcccggct ggcacgcttg ccggccgccg gcgccgcggc
     1801 gcacatcgag tcgctgcgcg cgcgcctgtc gcatgaatac gagagcttcc tcgcggccat
     1861 cgccgagtgg gcgcgcgtca aggagcagtg gctcggcgag aagaagcggg ccgtgcgcga
     1921 gcagtgggag caggttcagc tgcagcactg tctgcgcgag atcgagcgcc gcctgagcct
     1981 acagctgcgg cgcatgcgtc tgctgcaggc gcaactggcc tgagcccgtc tcaacctgca
     2041 atcgcgctcg ctgagcggcg ccggcactga gccggctgcg gctcgaggcg tgcgccgccc
     2101 gcggttggca cggcctgcgc gcttcgtgca ccatgcaccg catggggcgc atcttcgaag
     2161 tccgcaagca cacgatgttc gcgcgctgga accgcatggc gaagcagttc gcgcgcatcg
     2221 ccaaggacat caccatggcc gtgaaggccg gcggtccgga ccccaccgcg aacccgacgt
     2281 tgcggcgtgt catccacaat gcccgcgccg tcaacatgcc gaaggacaag atcgaggcgg
     2341 ccatcaagcg cgcctccggc cgcgacgcca cccattacca gcaagtgctc tacgagggct
     2401 atgcgcccta cggcgtggca ttgctggtgg agaccgccac cgacaacccg acacgcaccg
     2461 tggctgccgt gcgcaacatc atcaccaagg gtggcggcaa cctcgccacc aacggcagtg
     2521 tcagcttcat gttccgcaag atgggcgtgt tccgcctcga cccgcagggg ctcgaccagg
     2581 acgagctcga gctgtatctg atcgatcatg gcctggagga gatgggcgag agcaccggcg
     2641 agaagggcga gccgcaactg gtgatccgct gcgggttcgc cgactttggg cggatgcagg
     2701 aagcgctcga ggcgcgcggc atcgcggcgc tctctgccga gcacgagtac atctgcacgg
     2761 tgcccaccga gctcgccgag gagaaggcga ccgaggtgct cacgctgatc gatcgtctcg
     2821 agcaggacga cgacgtgcag aaggtcttcc acacgctcgc gtgagccctg ccacaccatg
     2881 ccggaccatc cacacgccta ccgcctgcaa cccgcccgcg ccggcgatgc cgcgttgctc
     2941 gccgcgatgt cggcggcgct catcgagcag ggcctgaagc cggcctgggg cgcggcgcgc
     3001 atccgctggc atgtgcgcga tgccgacagc gtggtgctca ccgcgcggct cggagccgcg
     3061 attgccggct ttgcgatcat gcgctacggc gaagatgtcg cgcacctcaa cctgctcgcg
     3121 gtcgcgcccg cccctcgccg ccgcggggta gcgcgcg
//
LOCUS       DATDMP010000505         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_969369, whole genome shotgun sequence.
ACCESSION   DATDMP010000505 DATDMP010000000
VERSION     DATDMP010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969369"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="VI195_08840"
     CDS             <1..1595
                     /locus_tag="VI195_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6824534.1"
                     /translation="APRRYDAIVMGAGHNGLVAACYLARAGLAVLVLERNRYIGGAAV
                     SRRLYDGFTYSNCSYVCSLLRTEIIRALELPRFGLQIIPYEGGCTLMRSGGHLAVYDN
                     HDAQRREIARHSRRDAESYERYVCDMLRHCRFIRPLLLRTPPDPTSLRPRDLGGLRWL
                     ARHFHGLGEARMYDTLRFLTMSCADMLEEYFESEIVKAHLAGSSIIGTALGPRSPGSA
                     YVLLHHYMGSIDGAVGAWGFARGGMGAITQALAASLAASGGTLRAAAPVEQILVRNGR
                     ATGVVLEGGEEIPARLVVSNLDVKRTFLGSIAAGHLPGEFLETVRRFKIRGSSGKLNI
                     ALDGLPEFPAIPPGSPCTRGDLHVTDSIDMLERAYDDWKEGRWSRSPYLDVLIPSQID
                     PTMAPDGKHYMSVFVQYCPYRLADGEWTGAKRQAFGETVLDAIARHSPNFRALVLHAE
                     IRTPQELENEVGLTEGNIFQGELTFDQLFFNRPVPGCAQYRAPLHGLYLCGSSTHPGG
                     GVMGAPGANAARTILADLRRAP"
     gene            1592..>2078
                     /locus_tag="VI195_08845"
     CDS             1592..>2078
                     /locus_tag="VI195_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6824535.1"
                     /translation="MSARHDCIVVGGGHNGLTCAAYLARSGRSVLVLEAAERLGGAAV
                     TRDFAPGFRVSACAHLLHLMPARLMRELDLAAHGLRLTTPLPTVALAAAGPHLAVEPA
                     DPAALAAHSRADAAAYPAYRERMSRLAAALRPLLAQVPPRLASGSWRDRAALLGLGLR
                     LR"
BASE COUNT          275 a          765 c          730 g          308 t
ORIGIN      
        1 gtgcgccgcg gcgctatgac gcaatcgtca tgggcgccgg ccacaacggc ctggtggcgg
       61 cctgttatct ggcgcgcgcc ggactcgcgg tgctggttct cgagcgcaac cgctatatcg
      121 gcggcgcggc ggtgagccgg cggctctacg acggcttcac ctactcgaac tgctcctacg
      181 tgtgcagcct gctgcgcacc gagatcatcc gtgcgctcga gctgccgcgc ttcgggctgc
      241 agatcatccc ctacgagggc ggctgcaccc tgatgcgcag cggcgggcac ctggccgtgt
      301 acgacaacca cgacgcccag cggcgcgaga tcgcgcgcca ctcgcggcgc gacgccgaga
      361 gctacgagcg ctacgtgtgc gacatgctgc gtcactgccg cttcatcagg ccgctgctgc
      421 tgcgcacgcc tccggatccc acctcgctgc gaccgcgcga cctcggcggg ctgcgctggc
      481 tggcgcggca cttccacggg ctcggcgaag cgcgcatgta cgacacgttg cgcttcctca
      541 ccatgagctg tgccgacatg ctggaggagt acttcgagag cgagatcgtc aaggcgcatc
      601 tcgccggcag ctcgatcatc ggcacggctc tcgggccgcg gtcgccgggc agcgcctacg
      661 tgctgctgca tcattacatg ggcagcatcg acggtgcggt cggcgcctgg ggcttcgccc
      721 gcgggggcat gggcgcgatc acccaggcgc tcgccgcctc gctcgcggcg agcggcggca
      781 cgctgcgcgc cgccgccccg gtcgagcaga tcctggtgcg caacgggcgc gcgaccggcg
      841 tggtgctcga gggcggggag gagatcccgg cgcggctggt ggtctcgaat ctcgacgtca
      901 agcgcacctt cctcggcagc atcgcagcgg ggcacctgcc cggggaattc ctcgagacgg
      961 tgcggcgctt caagatccgc ggctcttccg gcaagctcaa catagcgctc gacggcctgc
     1021 ccgagttccc ggccattccg cccggctcgc cctgcacgcg cggcgacctg cacgtgaccg
     1081 acagcatcga catgctggag cgcgcctacg acgactggaa ggaaggccgc tggtcgcgct
     1141 caccctatct cgacgtgctg atcccgtcgc agatcgatcc caccatggca ccggacggca
     1201 agcactacat gtcggtgttc gtgcagtact gcccgtaccg gctggccgac ggcgagtgga
     1261 ccggcgcgaa gcgccaggcc ttcggcgaga cggtgctcga tgcgatcgcg cgtcacagcc
     1321 cgaacttccg cgcgctggtc ctgcacgccg agatccgcac gccccaggag ctcgagaacg
     1381 aggtcggcct caccgagggc aacatcttcc agggcgagct cactttcgat cagctgttct
     1441 tcaaccgtcc ggtgccgggc tgcgcgcagt accgcgcgcc gctgcacggg ctttacctgt
     1501 gcggctccag cacccacccg ggcggcggcg tgatgggcgc accgggcgcc aatgccgcgc
     1561 gcacgattct cgccgacctg aggcgcgcgc cgtgagcgcg cgccacgact gcatcgtcgt
     1621 cggcggcggg cacaacggtc tcacctgcgc ggcctatctg gcacgcagcg gccgctcggt
     1681 gctggtgctg gaagcggccg agcgcctggg cggcgccgca gtgacgcggg acttcgcgcc
     1741 gggattccgg gtatcggcgt gcgcgcatct gctgcacctg atgcccgcga gactgatgcg
     1801 ggagctcgac ctcgcggcgc acggactgcg gctgaccacg ccgctcccca ccgtggcgct
     1861 ggcggcggcg ggcccgcatc tcgccgtgga gccggccgac cccgcggcgc tcgccgcgca
     1921 ctcgcgcgcg gacgccgccg cctacccggc ctaccgcgag cgcatgagca ggctcgcggc
     1981 ggcgctgcgg ccgctgttgg cgcaggtgcc gccgcggctc gctagcggct cgtggcgtga
     2041 ccgcgccgcg ctcctcggtc tcgggctgcg gctgcgcg
//
LOCUS       DATDMP010000506         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1303992, whole genome shotgun sequence.
ACCESSION   DATDMP010000506 DATDMP010000000
VERSION     DATDMP010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303992"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VI195_08850"
     CDS             complement(<1..904)
                     /locus_tag="VI195_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6824536.1"
                     /translation="MSTEAAALIREANALRQRGLLIEAEAAYKRILSRWPALPDCWFN
                     LGVVQRRAGNFPAALLSYQEALARGIEGPEEVHLNRAVIYTRLRQDDAAESELREALK
                     ANPSYLPALLNLANLHEDHARREDAQVLYRQALALDHNCFLALARLANLQAPPACDES
                     LIARLRAALQNPAATAADRADVGFALGRVLDATGHYAAAFAAYEAANRESRTAAMPIG
                     LYYDRAAQERVTEQLIAVPLPKPLAAAPAGPSRPRAIFVCGMYRSGSTLAEQLLAGHP
                     EVAAGGELDLLPALIANELLPFPES"
     gene            complement(925..1608)
                     /locus_tag="VI195_08855"
     CDS             complement(925..1608)
                     /locus_tag="VI195_08855"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY6824537.1"
                     /translation="MTTLTTRPFTSRTALDTALAQRLSAALGAAGPSAVMLSGGTTPL
                     PAYRALGARVLPHDERLHILFSDERYVPSDSEASNYQRARPLLDALALPPQRLLRVRT
                     ELPLEQAALQYEQALDALLKAGIRITLGLLGLGADGHTASLFSADDLARTSGRLAIPV
                     QRPDGMSGVSVTPGLLAQVEALLFVVAGGGKHEALQALEAHAANLTAWRAVQDCARVE
                     LWVHEAVEA"
     gene            complement(1634..>2392)
                     /locus_tag="VI195_08860"
     CDS             complement(1634..>2392)
                     /locus_tag="VI195_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6824538.1"
                     /translation="ANADSIRVQLADGRVADARIVGRDPDTDLAVLKIDLTPLPVATI
                     GRSDRLKVGDVVLAIGNPIGLSHTVTHGIVSAVSRQQLGIAPLEDFIQTDAPINFGNS
                     GGALIDSSGALVGINTAIVAKNIGVEGIGFAIPVNMVRGVLSDIIAHGRVIRGWIGIV
                     PEDVPEEQVQQLGLGQPAVMIENLYVGSPAQHAGLQPGDLLLGIDGTAPHSAQDALGR
                     IASRQPGTSVLLRGMRGGRVFEVRAQVGERPRSG"
BASE COUNT          373 a          816 c          834 g          369 t
ORIGIN      
        1 gcgactcggg gaaaggcagc agctcatttg cgatcagtgc aggcagcagg tcgagctcgc
       61 ctcctgcggc aacttcgggg tgcccggcaa gcagctgctc ggccagcgtc gagccggagc
      121 ggtacatgcc acacacgaag atcgctcgcg gccgtgatgg cccagcaggt gcagctgcta
      181 gcggcttcgg caggggcaca gcgatcagct gttctgtgac acgctcctgc gcggcgcggt
      241 cgtagtacaa cccgattggc atcgcggccg tgcggctttc tcggttggcc gcctcatacg
      301 cggcgaacgc cgcagcatag tggccggttg catcgagcac tcgaccgagt gcgaaaccga
      361 catccgcccg atcggcagcg gtggcggccg ggttctggag cgccgcgcgc agccgcgcga
      421 tcagactctc atcgcaggct ggcggagcct ggagattcgc gagtcgcgcg agcgccagaa
      481 agcagttgtg gtcgagggcc agtgcctgcc ggtagagcac ctgggcatcc tcgcgccgcg
      541 cgtgatcctc gtgaaggttc gcaagattca gcagcgccgg aagatacgag gggttcgcct
      601 tcagagcctc acgcagctcg ctttccgctg cgtcgtcctg gcgcagtctt gtgtagatga
      661 ccgcccggtt gaggtgcacc tcctcgggcc cctcaattcc gcgtgcgagc gcctcctggt
      721 aggagagcag cgctgcgggg aagttacccg cgcgacgctg caccaccccc aggttgaacc
      781 agcagtccgg cagggctggc cagcggctga gaatgcgctt gtacgccgct tccgcctcga
      841 tgagcagtcc tctctgccgc agagcgttcg cctcgcggat cagcgccgcc gcctcagtgc
      901 tcatgggcgg cgaggatacg ttaatcaggc ctcgacggcc tcgtgcaccc acagctcgac
      961 ccgggcgcag tcctgcacgg cgcgccaggc ggtgagattc gcagcgtggg cctcgagcgc
     1021 ctgcagcgct tcgtgcttcc cgccgccggc caccacgaag agcagtgcct ccacctgcgc
     1081 gagcagcccc ggcgtcacgc tcaccccaga catgccatcg ggccgctgca ccgggatggc
     1141 gaggcgaccg ctcgttcgag cgaggtcgtc cgcggagaag agcgatgcgg tatggccgtc
     1201 ggcaccgagc ccgaggaggc cgagagtgat gcggatgccc gctttcagca gagcatcgag
     1261 tgcctgctcg tactgcagcg ctgcctgctc gagcggcagc tcggtgcgca cccgcagcag
     1321 ccgctgcggc ggcagtgcga gcgcatcgag gaggggccgc gcgcgctgat agttgctcgc
     1381 ctccgagtcg ctcggcacat aacgctcgtc ggagaagaga atgtgcagcc gctcatcgtg
     1441 cggcaggacg cgcgcgccga gtgcacggta cgcgggcagc ggggtcgtgc cgcccgagag
     1501 catgacggcg ctcggtcccg ccgccccgag cgccgccgac agccgctgcg cgagtgccgt
     1561 gtcgagggca gtgcgcgagg tgaaggggcg ggtggtgagt gtggtcatgg cgatcctcca
     1621 tggtagtgcc gcatcagccg ctgcgcggac gctcgcccac ctgtgcccgc acctcgaaca
     1681 cgcggccgcc gcgcataccg cgcagcagca cgctcgtgcc cggctgacgg ctggcgatgc
     1741 gcccgagcgc atcctgggca ctgtggggcg ccgtgccatc gatgcccagg agcaggtccc
     1801 cgggctgcag ccccgcgtgc tgcgcgggac taccgacgta gagattctcg atcatgaccg
     1861 ccggctgccc gagcccgagc tgctggacct gctcctccgg cacatcctcg ggcacgatgc
     1921 cgatccagcc gcggatgacc cggccgtggg cgatgatgtc actcagcacc ccgcgcacca
     1981 tgttcaccgg aatggcgaag ccgatgccct cgacgccgat gttcttggcg acgattgccg
     2041 tgttgatgcc gaccagcgca ccgctcgagt cgatgagcgc gcccccggag ttgccgaagt
     2101 tgatcggcgc gtccgtctgg atgaaatcct cgagcggggc gatgccgagc tgctggcggc
     2161 ttactgcgct cacgatgccg tgtgtcacgg tgtgcgaaag cccgatcgga ttgccgatgg
     2221 cgagcaccac atcgccgacc ttcaggcggt cggagcgccc gatggtcgcg accggtagcg
     2281 gcgtcagatc gatcttgagc accgccaggt cggtgtccgg atcacgcccg acgatacgcg
     2341 catcggccac acggccgtcg gcgagctgca cacggatcga gtcggcgttg gc
//
LOCUS       DATDMP010000507         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1304330, whole genome shotgun sequence.
ACCESSION   DATDMP010000507 DATDMP010000000
VERSION     DATDMP010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304330"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VI195_08865"
     CDS             complement(<1..441)
                     /locus_tag="VI195_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY6824539.1"
                     /translation="MSLAVSGGRHLQALLGSLGRLARNPLATLLTLLVIALALALPTA
                     LRLFVTNAQAATGNFANAVDLSVFLKIDVPLAKAQQLAQAAQRRHDVASVTLIPAAQG
                     LEDFRTYSGFGDALVALKENPLPHVLHVRPQSEDSSAAALEALRS"
     gene            complement(438..1130)
                     /gene="ftsE"
                     /locus_tag="VI195_08870"
     CDS             complement(438..1130)
                     /gene="ftsE"
                     /locus_tag="VI195_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY6824540.1"
                     /translation="MIVFDRVSKRYPNGRDALSNVSFSIHTGEMVFLTGRSGAGKSTV
                     LKLIALLERPTRGAVMVAGRNTQTLKARHIAAFRRRIGVVFQDHKLLADRPVYDNVAL
                     PLAVAATPLKEIDRRVRAALDQVGLLGKERVLPLELSVGEQQRVGIARAVVSKPPLLI
                     ADEPTGNLDPDLSLEVMRLFRRFQDVGVTVVVASHDHTLVREFGQRVIVLESGQVQGD
                     AADPLPSVLAYR"
     gene            complement(1127..>2149)
                     /gene="ftsY"
                     /locus_tag="VI195_08875"
     CDS             complement(1127..>2149)
                     /gene="ftsY"
                     /locus_tag="VI195_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY6824541.1"
                     /translation="RIGGGRYNAPAMFRRAPENAPLEDRPGFLRRLRSRLNRGDSWLT
                     ADLGDLLRGRQIDAAILEELETRLITADVGVESSERILEGLRQRVARSELSDARALIS
                     ALREAIVGIIAPVAAPLRIDSARRPFVILVVGVNGSGKTTTIGKLALRCSAEGRSVLL
                     AAGDTFRAAAIEQLKVWAERSGADFAAQLAGADPGAVIFDALAAARARGSDVVLADTA
                     GRLHSQSHLMEELKKVKRVIQRFDPSAPHEVLLVLDANQGQNALVQARQFHQALGVTG
                     LALTKLDGTAKGGIVIAIANELRIPIRYIGIGEGPEDFGEFDAAAFASALTEGPERRG
                     SEAGAA"
BASE COUNT          348 a          750 c          724 g          327 t
ORIGIN      
        1 gctgcgcagg gcctcgagcg cagcggcgga gctgtcctcg gactgcggcc gcacgtgcag
       61 cacatgcggc agtggattct ccttgagcgc caccagcgca tcgccgaatc ccgaataagt
      121 gcgaaagtcc tcgagcccct gtgcggccgg aatcagcgta acggacgcca catcgtggcg
      181 ccgctgcgcc gcctgcgcca gttgctgggc cttggcgagc ggaacgtcga tcttgaggaa
      241 caccgacaga tcgaccgcgt tggcgaagtt gcccgttgcc gcctgcgcgt tggtcacgaa
      301 cagccgcagc gccgtcggca gagcgagcgc cagtgcgatc accagcagcg taagcaaggt
      361 cgcgagcggg ttgcgggcaa gccgcccgag cgagcccagc agggcctgga ggtggcgccc
      421 acccgagacc gcgagactca tcggtacgcc agtaccgagg gcagtgggtc ggcggcatcg
      481 ccctgcacct gaccgctctc gagcacgatc acccgctggc cgaactcgcg caccagggta
      541 tggtcgtggc tcgcgaccac caccgtgacc ccgacatcct ggaagcgccg gaacaaccgc
      601 atcacctcga gcgacagatc cggatcgaga ttgcccgtgg gctcatcggc gatcagcagc
      661 ggaggtttgc tgacgacggc gcgggcaatg ccgacgcgct gctgctcgcc gacggaaagc
      721 tcgagcggca gcacacgttc cttgccgagc aggcccacct ggtcgagcgc cgcgcgcacg
      781 cgccggtcga tttcctttag aggggtcgcc gccacggcaa gcggcagcgc gacattgtcg
      841 tacacgggcc ggtcggccag cagcttgtga tcctggaaga ccacaccgat gcggcggcgg
      901 aaggcggcga tatgccgggc cttcaaggtt tgggtattgc gtcccgcgac catgacggcg
      961 ccgcgggtgg gacgctcgag cagcgcgatc agcttcagca ccgtgctctt gccggcgccg
     1021 gagcggccgg tgaggaacac catctcgccg gtatggatgc tgaagctgac gttgctgagc
     1081 gcatcgcgcc cgttcgggta gcgcttgctc acccggtcga agacgatcat gcggcgcccg
     1141 cctcgctgcc gcgccgttcc ggtccctcgg tgagcgcgct cgcgaatgct gccgcgtcga
     1201 actcgccgaa gtcctcggga ccctctccga tcccgatgta gcggatcgga atcctgagct
     1261 cattggcgat cgcgatgact atgccaccct ttgcggtgcc atcgagcttg gtgagcgcga
     1321 gtccggtcac gcccagcgcc tggtggaact gccgcgcctg cacgagcgcg ttctggccct
     1381 gattggcatc gagcaccagc agcacctcgt gcggcgcact cggatcgaag cgttgtatca
     1441 cgcgcttcac cttcttcaac tcctccatga ggtgcgactg gctatgcaac cggcctgcgg
     1501 tgtcggcaag caccacgtcg ctgccgcggg cgcgcgcggc ggcgagcgca tcgaatatca
     1561 ccgcgcccgg gtcggcgccg gcgagctgtg cggcgaagtc cgcaccgctg cgctccgccc
     1621 acaccttcag ctgctcgatc gccgcggcgc gaaaggtgtc gccggcggcg aggagcaccg
     1681 agcggccttc ggcggaacaa cgcagcgcca acttgccaat ggtggtggtc ttgcccgagc
     1741 cgttcactcc caccaccagg attacgaacg gccgccgcgc gctgtcgatg cgcagcggcg
     1801 cagcgaccgg cgcgatgatc ccgacgatcg cctcgcgcag cgcgctgatg agcgcgcggg
     1861 catcgctcag ctcggatcgc gccacgcgct gccgcagacc ttcgaggatg cgctcgctcg
     1921 attccacccc gacatccgcc gtgatcagcc gcgtctcgag ctcctcgagt atcgcggcgt
     1981 cgatctgccg tccgcgcagc agatccccca gatcagcggt gagccacgaa tcgccgcgat
     2041 tcaggcggct gcgcaggcgg cgcaggaagc cggggcggtc ctcgagcggt gcgttttcgg
     2101 gagcgcggcg gaacatggcc ggtgcattgt accggccgcc accaatcct
//
LOCUS       DATDMP010000508         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2193633, whole genome shotgun sequence.
ACCESSION   DATDMP010000508 DATDMP010000000
VERSION     DATDMP010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193633"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..2231)
                     /gene="recG"
                     /locus_tag="VI195_08880"
     CDS             complement(147..2231)
                     /gene="recG"
                     /locus_tag="VI195_08880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY6824542.1"
                     /translation="MARAHSDPSPITHHSSLAGLSRSTQERLVRLGIHSKFDLVLHLP
                     LRYDDETRLYSLSEAPAGQPVLVEGRVLKCNVQYRPRRQLVCHIEDGSGVLTLRFFNF
                     YQSQQNQLGEGARVRAFGEIRYGFHGPEMIHPRYRVVQDDLPVAQALTPVYPTTAGLT
                     QDALRRLIARALADCDLGDTLPQRTRQTLGLPAFEESVMLLHNPPPQIPRELLQERRH
                     PAWRRVKFDELLAQQLSMRQHYRRRKAAGAPALKPRGQAARALRDMLPFELTRAQHRA
                     LAEIRGDLARPYPMQRLLQGDVGSGKTVVAALAALQCVENGYQVALMAPTEILAEQHH
                     AQFARWFDLLGIPLAWIAGGTRKNERKQILDRISRGEAMVAIGTHALFEEEVRFKNLG
                     LAIIDEQQRFGVHQRLALRMKGTRPNRNAQPHQLMMSATPIPRTLAMSYYADLDVSVI
                     DEMPPGRAPVATRLVSEARRGEVIRRIRDACMAGSQAYWVCPLIEESEVLQLQTALDT
                     HAVLAATFPELKLGLVHGRLKPADKAQVMAAFKDGAVQLLVATTVIEVGVDVPNAPLM
                     VIEHAERMGLAQLHQLRGRVGRGAETSVCILLYQNPLTPTARERLKIIFENRDGFEIA
                     RHDLKLRGPGEILGARQSGVPMLRFADLEADVDLLDAARGEAERLLREEPDAARDHLA
                     RWLGARHDYLKA"
     gene            complement(2251..2631)
                     /locus_tag="VI195_08885"
     CDS             complement(2251..2631)
                     /locus_tag="VI195_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6824543.1"
                     /translation="MAKNVVHTDAAPKAIGTYSQAVRCGQTVYLAGQIGLEPATMEMV
                     AGVGPQIHRVFQNLQAVARAAGGDLKDVVKLTVYLTDLGNFALVNETMAQYFSAPYPA
                     RAAVGVASLPRAAQVEIDAIMHLE"
BASE COUNT          458 a          940 c          908 g          495 t
ORIGIN      
        1 tggagcacga gctcgacccg gaatgccgtg caacgctccc gcacgcggtc agtcagcgag
       61 ccgcggtcaa tcagccagcg ccggtagcgg ccggcactcg cgccgagacg accgcgccat
      121 tcgttatcac gcatcattcg cgtctgctat gccttgagat agtcgtgtct tgcgccaagc
      181 caacgcgcca gatggtcgcg cgccgcatcg ggctcctcgc gcagaaggcg ctcggcctct
      241 ccgcgagccg cgtcgagaag atccacatcg gcttcgaggt cggcaaagcg gagcatgggc
      301 acgccgctct ggcgcgcgcc gaggatttcc ccggggccgc gaagcttcaa gtcgtggcgc
      361 gcaatctcga agccgtcacg attctcgaag atgatcttca ggcgctcgcg cgcggtcggc
      421 gtcagcgggt tctggtagag gagaatgcag acgcttgtct ccgcgccgcg cccgacgcgc
      481 ccgcgcaact ggtgcagctg cgccagcccc atgcgctccg cgtgctcgat caccatcaat
      541 ggcgcgttcg gaacgtccac tccgacctcg atcacggtgg tcgcgacgag cagttggacc
      601 gctccgtcct tgaatgcggc catgacctgc gccttgtccg ccggcttgag ccgcccgtga
      661 acgagcccga gcttcagctc gggaaaggtt gccgcgagca ccgcgtgggt atcgagcgcc
      721 gtttgcaatt gcaggacttc ggattcttca atcaagggac aaacccagta cgcctggctg
      781 ccggccatgc aagcgtcccg gatgcgccgg atcacctccc cgcggcgcgc ctccgacacg
      841 agccgcgtcg cgaccggggc gcgcccgggc ggcatctcgt cgatcacgga aacgtcgaga
      901 tcggcgtagt agctcatcgc gagcgtgcgc gggatcggcg tcgcgctcat catcagctgg
      961 tgcggctgcg cattgcggtt cggacgcgtg cctttcattc gcagcgcaag acgctgatgc
     1021 acgccgaaac gctgctgctc gtcgatgatc gcgagcccga gattcttgaa gcgcacttcc
     1081 tcttcgaaca gcgcgtgggt gccgatcgcg accatcgctt cacctcgaga aatcctgtca
     1141 agtatttgtt ttctttcatt ttttcttgtc ccgccggcaa tccatgcaag gggaattccg
     1201 agcagatcga accagcgcgc gaattgcgcg tggtgctgct cggcgagaat ctccgttggc
     1261 gccatcagcg ccacctggta gccgttctcc acgcactgca gcgcggcgag cgcggccact
     1321 accgtcttgc cggagccgac atcgccctgc agcaaccgct gcatcggata aggccgggcg
     1381 aggtcgccgc gtatctcggc gagcgccctg tgctgcgcgc gcgtcaactc aaagggcagc
     1441 atgtcgcgca gcgcgcgcgc tgcctgcccg cgcggtttca gcgcgggtgc cccggcggcc
     1501 ttgcggcggc ggtagtgctg gcgcatcgag agctgctgcg cgagcagttc gtcgaacttg
     1561 acgcgccgcc acgccgggtg gcggcgttcc tgcagcagct cgcgcggaat ctgtggtggc
     1621 gggttgtgca ggagcatcac cgattcctcg aatgccggca gcccgagcgt ctgccgcgtc
     1681 ctctgcggca acgtatcccc gagatcgcag tcggccagcg cgcgcgcgat caggcgccgc
     1741 agcgcgtcct gcgttagccc cgcggtggtg ggatacaccg gtgtcagcgc ctgcgccaca
     1801 ggcaggtcgt cctgcaccac gcgatagcgc ggatggatca tctccggtcc atggaagccg
     1861 tagcggattt cgccaaacgc gcgcacgcgc gcgccctccc cgagctggtt ctgctgactc
     1921 tggtagaagt tgaagaagcg caacgtcagc acgccgctgc cgtcttcgat gtggcagacc
     1981 agctgacgcc gcgggcggta ctgcacattg cacttcagca ccctgccctc caccagcacc
     2041 ggttgtcccg ccggagcctc gctcagggaa tagagccgcg tctcatcgtc gtagcgcagc
     2101 ggcaggtgca ggacgaggtc gaacttgctg tggatgccca gccggacgag ccgttcctgc
     2161 gtcgaacggc tcaggccagc cagtgatgag tgatgagtaa tgggtgatgg gtcagaatgc
     2221 gcccgcgcca tgtcttgcac ccatcacccg tcactcgaga tgcatgatgg cgtcgatctc
     2281 gacctgcgca gcgcgcggca ggctcgcgac gcccaccgcg gcgcgcgccg gatagggcgc
     2341 cgagaaatac tgggccatgg tctcgttcac caacgcgaag ttgcccaggt cggtgagata
     2401 gacggtcagt ttcacgacgt ccttgagatc gccgcctgct gcgcgcgcca ccgcctgcag
     2461 gttctggaac acgcgatgga tctgcgggcc gaccccggcg accatttcca tcgtcgcagg
     2521 ctcgagtccg atctggccgg cgaggtaaac ggtctgcccg cagcgcactg cctgtgaata
     2581 ggtgccgatc gccttgggcg cggcatccgt atggacgacg ttcttggcca tggctcctcc
     2641 cttgagtttc ctgttcaaga cggcagcttc agcacgtact tggcgaggat gttccgctgg
     2701 atctcgctcg agccgccgta gatggtagcg ggacgcgaat agtagaacgg gctcaggaaa
     2761 tcggtctcca cgccggcgac gtcctgcgcg ccggcgatga c
//
LOCUS       DATDMP010000509         2933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_526241, whole genome shotgun sequence.
ACCESSION   DATDMP010000509 DATDMP010000000
VERSION     DATDMP010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526241"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VI195_08890"
     CDS             <1..682
                     /locus_tag="VI195_08890"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF023246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEY6824544.1"
                     /translation="STQHRGVQPDVPLASVLDTKEIGESALESALPWDRIAGVPFKAS
                     AVPAPPVAALATEEGERAQHDPDYKWLVSDIAAIDSVREQHTVSLNLKTRREERARME
                     RERLERENSRRAAKNLAPLKSVEELEKAKDEAADVVLEQAAQVMADMVGGVHPGTPQQ
                     KTARADGHFSGVPAKCRVLLRDVCLACRVGRDFVGCFSAAARETDPRCPRRARSASAR
                     RPHPARAP"
     gene            complement(591..1658)
                     /locus_tag="VI195_08895"
     CDS             complement(591..1658)
                     /locus_tag="VI195_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824545.1"
                     /translation="MKALRQLGFLKLSLRPPDAPPEEDHRVLALFRNRAELKKAYGDL
                     QEESFRLKDLVKQQEGATQRVQEMLNALEGRLGIAETAYPALVFYQLRRLWQSGRELI
                     TAFVADLARQQEERERRAHLAQHNREQFARRQSAEAELGNAQALHAEASAQLATLEGE
                     RAALTRFWHYFRRRALERRIAAAQVALAGIDSSVTQTQARVDEIARETSPEFPGLSLQ
                     SRRSINLAGIACAEVLCLRLTELKTPLVELARQAIGRREPGNEYGTPKECVLLMGEIA
                     RAHRLIAERVGLAGEIRDRSERLKSLARYRGPKDTAPQVECLAPSEGDVLTVPAQGED
                     AARLPNVLAEDTWDLFRVLLR"
     gene            complement(1687..2205)
                     /locus_tag="VI195_08900"
     CDS             complement(1687..2205)
                     /locus_tag="VI195_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HEY6824546.1"
                     /translation="MLLADTTTIVSWRARPRSFVALMGLYESNFIRLGWLAGTLRELR
                     GRYLSSVHGDCDLVLTVTERSPYTSTVNLTYLLPEAHGPRRYPDMRVRIYHDAHLVEA
                     QPWHSGHPPGRGAAPPGGTRPQASARARRGLPERELDQRWARNMMLNKWLEYCVERGH
                     RFSSRTEQRAGA"
     gene            complement(2377..>2933)
                     /locus_tag="VI195_08905"
     CDS             complement(2377..>2933)
                     /locus_tag="VI195_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEY6824547.1"
                     /translation="LFSAFVGPLERELNTRSTHRAGRAHGIADLAAYTTRINATNFAL
                     ANDDGSGGDYAHADVVLVGVSRVGKTPTCVYMALQYGVFAANYPLTDEDLEAGRLPVR
                     LEPFRRKLYGLTIRPERLQQIRSERRRDSRYASRAQVQYELRAADALFQRYSVPTLDT
                     SESSIEEIASRIMNSTGIERRLPL"
BASE COUNT          495 a          954 c         1021 g          463 t
ORIGIN      
        1 aagcactcag catcgcggag tgcagccgga cgtgccactc gcctcggtgc tcgacaccaa
       61 ggagatcggc gagagcgcgc tcgagtccgc gcttccctgg gaccgcatcg ccggtgtgcc
      121 cttcaaggcg agcgccgtgc ccgccccacc ggtggcggca ctcgcgaccg aagaaggcga
      181 acgcgcgcag cacgatccgg actacaagtg gctggtgtcg gacatcgcgg ccatcgactc
      241 ggtgcgcgag cagcacaccg tgtcgctcaa tctcaagacg cgccgtgagg agcgcgcgcg
      301 catggagcgt gagcggctcg agcgcgagaa cagccgccgc gcggccaaga acctcgcgcc
      361 gctgaagagc gtggaagagc tggaaaaggc caaggacgaa gccgccgacg tggtgctcga
      421 gcaggccgcg caggtgatgg cggacatggt ggggggcgtc catccaggga cgccgcagca
      481 gaagacggcg cgcgcggacg gacacttcag cggggtcccc gcgaaatgtc gcgtcttgct
      541 gcgcgatgtg tgcctggcct gtcgcgttgg gcgagatttc gtggggtgct tcagcgcagc
      601 agcacgcgaa acagatccca ggtgtcctcg gcgagcacgt tcggcaagcg cgcggcgtcc
      661 tcaccctgcg cgggcaccgt gagcacatcg ccctcggaag gcgcgaggca ctcgacctgc
      721 ggcgccgtgt ccttgggacc gcggtagcgg gcgagagact tcagccgctc gctgcgatcg
      781 cggatctcgc cggcgagccc gacgcgctca gcgatcagcc ggtgcgcgcg cgcgatctct
      841 cccatcagca gcacgcattc cttcggtgtg ccgtactcat tacccggctc gcgacggccg
      901 atcgcctgcc gggcgagctc gaccagcggc gtcttgagct ccgtcaagcg caggcacagc
      961 acctcggcac aggcgattcc cgcgaggttg atggagcggc gcgattgcag cgacagcccc
     1021 gggaactccg gggaagtctc gcgcgcgatc tcatcgaccc gcgcctgcgt ctgcgtgacc
     1081 gagctgtcga tgccggcgag cgccacctgc gcggcagcga tgcgccgttc gagcgcacgg
     1141 cgccggaagt agtgccagaa gcgagtgagc gcagcccgct cgccctcgag ggttgccagt
     1201 tgtgcgctcg cctccgcatg cagcgcctgg gcgttgccga gctcagcttc cgcactctga
     1261 cggcgggcga actgctcgcg gttgtgctgc gcgagatgag cgcgccgttc gcgctcctcc
     1321 tgctggcgcg cgagatcggc gacgaaggca gtgatcagct cgcgaccgct ctgccacagg
     1381 cggcgcagct ggtagaacac cagcgcggga taagccgtct cggcgatgcc cagacgcccc
     1441 tcgagtgcgt tgagcatctc ctggacgcgt tgcgtggccc cctcctgctg tttgaccagg
     1501 tccttgagac ggaacgactc ttcctgcaga tcgccgtagg cctttttcag ttccgcgcgg
     1561 ttgcgaaaca gcgccaacac ccgatggtct tcttcgggtg gggcgtcggg gggacgcagg
     1621 ctgagcttga gaaagccgag ttgtcgcaac gccttcatga gagatgcggc gcccagccgc
     1681 cccttctcat gcgccggcgc gttgctccgt gcgagatgag aagcgatgac ctcgctccac
     1741 gcagtactcc agccacttgt ttaacatcat gttacgggcc cagcgctgat ccagttcacg
     1801 ctccggcaag ccgcgccgag cgcgcgcgga cgcctgcggg cgggtgccac ccggcggtgc
     1861 cgctccccgg ccgggagggt gaccgctgtg ccacggctgc gcttcgacga gatgcgcgtc
     1921 atggtagata cgcacgcgca tgtcggggta gcggcgcggt ccgtgcgcct ccggcagcag
     1981 gtaagtgaga ttgacggtgc tggtgtacgg tgagcgctcg gtcacggtga gaacgaggtc
     2041 acagtctccg tgtaccgagg aaaggtagcg cccgcgcagt tcacgcaggg ttccggcgag
     2101 ccagccgagg cgtatgaagt tgctctcgta cagccccatg agcgcgacga agctgcgcgg
     2161 ccgggcccgc caggaaacga tcgttgtggt gtcagcaagc agcacgcccc gactataacg
     2221 cacaaggctg ctgctgcaag tgcgaacagg acgcgatttg cggtggcagc gtggcgcgta
     2281 ctgcttgcaa ttttcgtgcg acgtgcagcg gttacccgcg cacacagcaa acgtgcaaca
     2341 atgggtaact cggtggcgag cgaaccgcgc gctacgctag agcggcaagc gccgctcgat
     2401 gcccgtgctg ttcatgatgc ggctggcgat ctcctcgatc gaggattcgc tggtatcaag
     2461 tgttggcacg gaatagcgct ggaacagcgc atccgcggca cgcagctcgt actgcacctg
     2521 cgcgcgggag gcataacggc tgtcgcggcg ccgttcggag cggatctgct gcagccgttc
     2581 gggccggatg gtgagtccgt agagcttcct gcgaaaaggc tcgagccgta ccggcagccg
     2641 cccggcctcg aggtcctcat cggtaagcgg gtagttggcg gcgaacactc cgtactgcag
     2701 cgccatgtag acgcaggtcg gcgtcttgcc gacgcgcgac actcccacca gcaccacatc
     2761 cgcatgggca tagtcgccgc cgctgccgtc atcgttggcg agcgcgaagt tggtggcatt
     2821 gatgcgcgtc gtgtaggcag cgaggtcggc gataccgtgc gcgcgcccgg cacgatgcgt
     2881 ggagcgggtg ttgagctcac gctcgagcgg accaacaaac gccgagaaca gat
//
LOCUS       DATDMP010000510         3345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_193559, whole genome shotgun sequence.
ACCESSION   DATDMP010000510 DATDMP010000000
VERSION     DATDMP010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193559"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VI195_08910"
     CDS             <1..712
                     /locus_tag="VI195_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824548.1"
                     /translation="ARLNEARSGTMLDYIQAFTAAFPAAVDDFETLLTDNRIWKQRTV
                     NIGVVSPERAMQLGFSGAMLRGSGIAWDLRKKQPYEVYQRMDFDIPVGVNGDCYDRYL
                     VRVAEMRQTNRIIRQCIEWLRAHPGPTMIDDRKVRPPRREKMKEDMEALIHHFKFFTE
                     GFEVPEGDTYAAVEAPKGEFGVYLVSDGANKPYRLKCRAPGFAHLAALEEMVRGHMIA
                     DVVAVIGTQDIVFGEIDR"
     gene            709..1242
                     /gene="nuoE"
                     /locus_tag="VI195_08915"
     CDS             709..1242
                     /gene="nuoE"
                     /locus_tag="VI195_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY6824549.1"
                     /translation="MSERSSVGVGGTAMVSKLTLLSEQTRSEIDHWVAKFPPGRQRSA
                     SLAALRAAQEQNAGFLTADLMDAVAEYLKLPPIQVYEVASFYSMFATQPCGRHHVSIC
                     TNISCMLRGGEELLEHAQRKLGIRPNQSTADGRIFLKREEECLAACTGAPMMMVDHVF
                     HEHLTAEKLDAVLDALK"
     gene            1239..2567
                     /gene="nuoF"
                     /locus_tag="VI195_08920"
     CDS             1239..2567
                     /gene="nuoF"
                     /locus_tag="VI195_08920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY6824550.1"
                     /translation="MSDLESQYAERMNLTVFEPLKLERSWTLAAYRSIGGYDVWERLL
                     REKPPREQIIDQVKASGLRGRGGAGFPTGTKWSFMPRNSPVQKYLVCNSDESEPGTCH
                     DRDILRYNPHAVIEGLAIAGYATNSTIAYNYIRGEFLGEPVPRFEAALAEAYAAGLLG
                     HNVLGSGVDFDLHTFVGAGAYICGEETALLDSLEGKTGKPRFKPPFPANFGLYGAPTT
                     INNTLSYASVPTILRKGPAWFAGLGPPNSGGTMIYSMSGHIEKPRNIELPLGVPFADL
                     LALCGGVRGGRKLKAVIPGGVSVPVVPGEIMLKTNMDYDSVKAAGSALGTGAAIVMDE
                     TTCMVRVLERISRFYMAESCGQCTPCREGTGWMNRVLKRVLAGQARREELGLLLDVAN
                     RIEGHTICAFGDASAWPVQSFLKHYRHEFEYMIDHGGRSLVSNGAGARAA"
     gene            2586..>3345
                     /locus_tag="VI195_08925"
     CDS             2586..>3345
                     /locus_tag="VI195_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6824551.1"
                     /translation="MAESEVNIEVDGVPMKAHKGEMIIRATDRAGVYVPRFCYHEKLS
                     IAANCRMCLVEVEKAPKPLPACATPVAEGMKVFTKSPRAIGAQRATMEFLLINHPLDC
                     PICDQGGECELQDLAVGFGRDISRYHEGKRSVQDESLGPLIATDMTRCIQCSRCIRFT
                     EEIAGMQELGMIGRGEHMAVRSYIESTVNHELSGNVIDLCPVGALVSKPYRFSARAWE
                     MRAVPLISPHDAVGSNLFGHVLRGKLMRVVPRENE"
BASE COUNT          588 a         1112 c         1115 g          530 t
ORIGIN      
        1 ggcgcggctc aatgaggcgc gcagcggcac gatgctcgac tacatccagg ccttcactgc
       61 agcgtttccc gcggcggtgg acgacttcga gacgctgctc accgacaacc gcatctggaa
      121 gcagcgcacg gtgaatatcg gcgtggtgag tcccgagcgc gccatgcagc tcggcttctc
      181 cggtgccatg ctgcgcggct ccggcatcgc ctgggacctg cgcaagaagc agccctacga
      241 ggtctaccag cgcatggatt tcgatatccc ggtgggcgtc aacggcgatt gctacgaccg
      301 ctacctggtg cgggtggccg agatgcgcca gacgaaccgc atcatccgcc agtgcatcga
      361 gtggctgcgc gcgcacccgg gcccgacgat gatcgatgac cgcaaggtgc ggccgccgcg
      421 gcgcgagaaa atgaaggagg acatggaagc gctcatccac cacttcaagt tcttcaccga
      481 ggggttcgaa gtgcccgaag gcgacaccta cgccgcggtc gaagcgccga agggcgagtt
      541 cggcgtctac ctggtttccg acggtgccaa caagccctac cggctcaaat gccgcgcgcc
      601 gggctttgcg cacctcgccg cgctcgagga gatggtgcgc gggcacatga tcgcggatgt
      661 ggtggccgtg atcggcaccc aggacatcgt cttcggggaa atcgaccgat gagcgagcgc
      721 agcagcgtcg gcgtcggtgg caccgcgatg gtgagcaagc tcactctgct ctcggagcag
      781 acgcgcagcg agatcgacca ctgggtggcg aagttcccgc ccgggcggca gcgctcggcg
      841 tcgcttgccg cgctacgcgc tgcgcaggag cagaacgcgg gcttcctgac agcggacctg
      901 atggacgcgg tggccgagta cctcaagctg ccgcccatac aggtgtatga ggtcgcgagc
      961 ttctactcca tgttcgcgac ccagccatgc gggcgccatc acgtcagcat ctgcaccaac
     1021 atctcgtgca tgctgcgcgg cggggaggag ctgctcgagc acgcgcagcg caagctcggc
     1081 attcgtccga atcagagcac cgccgacggc cgcatctttc tcaagcgcga ggaggagtgc
     1141 ctcgcggcct gtacgggtgc gccgatgatg atggtggacc acgtgttcca cgagcacctc
     1201 acggccgaga agctcgacgc cgtcctggat gcgctcaaat gagcgacctc gagagccagt
     1261 acgccgagcg catgaatctc acggtgttcg agcccttgaa gctcgagcgc tcctggacac
     1321 tcgcagccta ccgcagcatc ggtggctacg acgtctggga gcggctgctg cgcgagaagc
     1381 ctccgcgcga gcagatcatc gatcaggtga aggcctcggg gctgcgcggc cgcggcggtg
     1441 ccggctttcc caccggcacc aagtggagct tcatgccgcg caactcaccg gtgcagaaat
     1501 acctggtgtg caattcggat gagagcgagc cgggcacctg tcacgaccgt gacatcctgc
     1561 gctacaaccc gcacgcggtg atcgagggtc tcgccatcgc cggctatgcg accaactcca
     1621 ccatcgctta caactacatc cgcggcgagt tcctcggcga gccggtgccg cgtttcgagg
     1681 cggcgctggc ggaggcctac gcggcgggac tgctcggcca caacgtgctc ggctcgggcg
     1741 tcgatttcga tctgcacacc ttcgtcggcg cgggcgctta catctgcggc gaggagacgg
     1801 cgttgctcga ctcgctcgag ggcaagaccg gcaagccgcg cttcaaaccg ccgttcccgg
     1861 ccaatttcgg cttgtacggg gcgccgacca ccatcaataa cacgctcagc tacgcctcgg
     1921 tgccgaccat cctgcgcaag ggcccggcct ggttcgccgg gctcggcccg ccgaactcgg
     1981 gcggcaccat gatctactcg atgtccggcc acatcgagaa gccgcgcaac atcgagctgc
     2041 cgctcggcgt tccctttgcg gatctgctcg cgctctgcgg cggcgtgcgc ggcgggcgca
     2101 agctgaaggc ggtgatcccc ggcggcgtct cggtgccggt cgtgcccggg gagatcatgc
     2161 tcaagaccaa catggattat gactcggtga aggcggcggg ctctgcgctc ggcaccggcg
     2221 ccgccatcgt catggacgaa accacctgca tggtgcgcgt gctcgagcgc atctcgcgct
     2281 tctacatggc ggagtcctgc ggtcagtgta cgccgtgccg cgaaggcacc ggctggatga
     2341 accgcgtgct caaacgcgtg ctcgccggcc aggcgcggcg cgaagagctc ggactgctcc
     2401 tcgatgtcgc taaccgcatc gaaggacaca ccatctgcgc cttcggcgat gcctcggcct
     2461 ggccggtgca gagcttcctc aagcattacc ggcacgagtt cgagtacatg atcgaccacg
     2521 gcgggcgcag cctggtgagc aacggcgcgg gggcgcgcgc ggcctgaggg gcgcaggaac
     2581 gacacatggc ggagagcgaa gtcaacatcg aggtcgacgg cgtgccgatg aaggcgcaca
     2641 agggcgagat gatcatccgc gccaccgacc gcgcgggcgt gtacgtgccg cgcttctgct
     2701 accacgagaa gctctcgatc gccgccaact gccgcatgtg cctggtcgaa gtggagaagg
     2761 cgccgaagcc gctgccggcg tgcgctacac cggtcgccga gggcatgaag gtgttcacca
     2821 agtcgccccg cgccatcggc gcgcagcgcg ccaccatgga attcctgctc atcaatcacc
     2881 cgctcgattg cccgatctgc gatcagggcg gggagtgcga gctgcaggac ctggcggtcg
     2941 gcttcggccg cgacatctcg cgctatcacg agggcaaacg ctcggtgcag gacgagagtc
     3001 taggtccgct catcgccacc gacatgaccc gctgcatcca atgcagccgc tgcatccgct
     3061 tcaccgaaga gatcgccggc atgcaggagc tcggcatgat cggccgcggc gagcacatgg
     3121 cggtgcgcag ctacatcgag agcacggtga accacgagct ttcgggcaac gtcatcgatc
     3181 tgtgtccggt cggcgcgctg gtctccaagc cttaccgctt cagcgcgcgc gcctgggaga
     3241 tgcgcgccgt gccgctcata tcgccgcacg atgccgtggg gagcaacctc ttcggccacg
     3301 tgctgcgcgg caagctgatg cgcgtggtgc cgcgcgagaa cgaat
//
LOCUS       DATDMP010000511         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2194200, whole genome shotgun sequence.
ACCESSION   DATDMP010000511 DATDMP010000000
VERSION     DATDMP010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194200"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VI195_08930"
     CDS             <1..285
                     /locus_tag="VI195_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY6824552.1"
                     /translation="DLVVYLQAPVDVLLERIARRGVEYEQYIDKGYLERLNEAYARFF
                     HEFDAAPLLIVNAASIDPISNQRDYDELLAAIRRMSRGRLYFNPLRSRAI"
     gene            354..1178
                     /gene="panB"
                     /locus_tag="VI195_08935"
     CDS             354..1178
                     /gene="panB"
                     /locus_tag="VI195_08935"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY6824553.1"
                     /translation="MYTHLQRDQSRPPVTLSTLARMKADGEKIACLTCYDASFAVLID
                     TADVDVVLVGDSLGMVIQGHHTTVPVTMDHMAYHSAAVARGLHRPFLIADLPFMSYPS
                     RDLALQHSVRLMQEGGAQMVKLESGGKQTDIVEFLAGHDIAVCAHLGLKPQSVHKTGG
                     FRVQGRERETAVRLLDEARRLESAGADIVLLECIPAPLGKSITAELHVPVIGIGAGPD
                     TDGQILVTYDMLDITTGRKPRFVRNFMEGASDNLDALKRYVRAVKSREYPGPEHCF"
     gene            1207..2049
                     /gene="panC"
                     /locus_tag="VI195_08940"
     CDS             1207..2049
                     /gene="panC"
                     /locus_tag="VI195_08940"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY6824554.1"
                     /translation="MTTIAAVREHVHGWRREGLRIAFVPTMGNLHAGHVSLIEAARRH
                     GQRFIASIFVNPMQFGPNEDFAHYPRTPREDERMLAAAGCSLMFMPDVAEIYPHGAER
                     ATRIEVPGLSRILDGEVRPGHFEGVSTVVAKLFHIVEPDVAVFGEKDFQQLTVIRRMV
                     ADLCMPVEIVGAPTVRDADGLAMSSRNQYLTPSERALAPKIYRALQAAAARLQAGDAD
                     FTGIERAGFQVLESAGFQPDYFSVRQAHDLSTATAAARELVILVAARIGKARLIDNVQ
                     VRRG"
     gene            2075..2512
                     /locus_tag="VI195_08945"
     CDS             2075..2512
                     /locus_tag="VI195_08945"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY6824555.1"
                     /translation="MSVIYPDLSTRPLNLTVEHRMHAPPAALYDAWTKGFERWFAAPG
                     TALMAPEVNRPFFFETQHEGERHAHYGRFLRLERHALVELTWVTAGGTHGAETVVTVE
                     LRSAGGGTQLRLTHAGFPDEALCQRHRDAWPMVLKHLDEVLRL"
     gene            complement(2481..>2903)
                     /locus_tag="VI195_08950"
     CDS             complement(2481..>2903)
                     /locus_tag="VI195_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEY6824556.1"
                     /translation="YLTIEHHSAIPVELRPAIGNRIFGCDDCQLVCPWNKFARKAAHP
                     DFKVRHSLDSTSLTELFRWTAQEFEERTLGSAIHRLGYVRWSRNIAVALGNAPASPEV
                     IEALEERREDGSPLVREHVEWALTRQRSLKGAAPHRDA"
BASE COUNT          483 a          981 c          933 g          506 t
ORIGIN      
        1 gacctcgtcg tgtacctgca ggcgccggtg gatgtgctgc tcgagcgcat tgccaggcgc
       61 ggggtcgagt acgagcagta catcgacaaa ggttacctcg agcggctgaa cgaggcctac
      121 gcacgcttct tccacgagtt cgacgccgcc ccgctcctca ttgtcaatgc tgcctccatc
      181 gacccgattt ccaaccaacg cgattacgac gagctgctgg cggccatccg ccgcatgagc
      241 cgcggccgcc tctatttcaa ccccctgcgc agccgcgcca tttaggccat cgggcccacc
      301 cgtccagggg cctttttgca ctgcaaagta gctgggctac actgttatga cctatgtata
      361 cccatctgca acgggatcag agccggccac ccgtgactct ctcgaccctc gcgcgcatga
      421 aggcggatgg cgagaagatc gcctgcctca cctgttatga cgcgagtttc gcggtgctca
      481 tcgatacggc ggatgtcgat gtcgtgctgg tgggggattc tctcggcatg gtcatccagg
      541 gccatcacac gacagtcccc gtcaccatgg accacatggc ttaccacagc gccgcggtag
      601 cgcgaggact tcatcggccg tttctgatcg ccgacctgcc gttcatgagc tacccctcgc
      661 gggatctcgc gctgcagcac tctgtgcgcc tcatgcagga gggcggcgcc cagatggtga
      721 agctcgagag cggcggcaag cagaccgaca tcgtcgagtt cctcgccggc cacgacatcg
      781 cggtgtgcgc ccacctcggc ctcaagccgc agtcggtgca caagaccggc ggcttccgcg
      841 tccaggggcg cgagcgcgaa accgctgtgc ggctcctcga tgaggcgcgg cggctggaaa
      901 gcgcgggcgc cgacatcgtg ctgctcgagt gcattccggc gccgctcggc aagtccatca
      961 ccgccgagct ccatgtgccc gtgatcggta tcggtgcggg ccccgacacc gacggccaga
     1021 tcctcgtcac ctacgacatg ctcgacatca ccaccggccg caagccgcgc ttcgtgcgca
     1081 acttcatgga aggcgcaagc gataacctcg atgcgctcaa gcgctacgtg cgtgcggtga
     1141 aatcccgcga gtaccccggg cccgagcact gcttctgaag tagagccggt tcgccgcttg
     1201 gataccgtga ccacgatcgc cgccgtacgc gagcacgtgc acggctggcg ccgcgagggt
     1261 ctgcgcatcg ccttcgttcc caccatgggc aatcttcatg ccgggcatgt gagcctgatc
     1321 gaggcggcgc gccgccacgg tcagcgcttc atcgccagca tcttcgtcaa cccgatgcag
     1381 ttcggcccga acgaggactt cgctcattac ccgcgcacac cgcgcgagga tgagcgcatg
     1441 ctagcggccg cgggctgcag cctcatgttc atgcccgatg tcgccgagat ctatccccac
     1501 ggcgcagagc gcgcgacgcg catcgaggtg ccgggactct cacgcattct cgatggggag
     1561 gtccgtccgg gacacttcga gggggtaagc acggtggtgg cgaagctctt ccacatcgtc
     1621 gaacccgacg tcgcggtctt cggcgagaag gacttccagc agctcaccgt cattcgccgc
     1681 atggttgccg atctctgcat gccggtcgag atcgtgggcg ccccgacggt gcgcgatgcc
     1741 gatggtctcg ccatgagctc gcgcaaccag tacctgaccc cgtccgagcg cgcgctggcg
     1801 ccgaagatct accgggcgct tcaggccgcc gcggcgcggc tgcaggcagg cgatgcggac
     1861 ttcaccggca tcgagcgggc cggcttccag gtactcgaga gcgcagggtt tcagccggat
     1921 tacttcagcg tgcggcaggc gcatgatttg agcaccgcca ccgccgccgc gcgcgagctc
     1981 gtgatcctgg tggccgcccg catcggcaag gcgcgcctca tcgataacgt gcaggtgcgg
     2041 cgcggctaga gtccgacagc gggagggaag tccgatgagc gtcatctatc ccgatctttc
     2101 gacccgcccc ctcaacctca ccgtcgagca ccgcatgcac gcgccacccg ctgcgctcta
     2161 tgacgcctgg accaaaggct tcgagcgttg gtttgccgcg cccgggacgg cactcatggc
     2221 cccggaggtg aaccggccgt ttttcttcga aacgcagcac gagggcgagc gccacgcgca
     2281 ctacgggcgc ttcctgcgcc tcgagcggca tgctctggtt gagctcacct gggtgacagc
     2341 cggcggcacg catggggccg agaccgtcgt gacggtcgag ctgcggtccg cgggaggtgg
     2401 cactcagctg cgcctgacgc atgccggttt tccggatgag gcgctctgtc agcggcaccg
     2461 cgacgcctgg ccaatggtgc tcaagcatct cgatgaggtg ctgcgccttt gagggaccgc
     2521 tgccgcgtga gtgcccattc gacgtgctcg cgcacgaggg gagagccatc ctcgcggcgc
     2581 tcctccagag cctcgatgac ctccggggat gcgggcgcat tgccgagtgc gaccgcgatg
     2641 ttgcgcgacc agcgcacgta gcccaggcga tgtatcgccg agcccagcgt gcgttcttcg
     2701 aactcctgcg ccgtccagcg aaagagctcg gtgagtgagg tcgagtcgag actgtggcgc
     2761 accttgaaat ccgggtgcgc cgctttgcgc gcgaatttgt tccaggggca cacgagctga
     2821 cagtcatcgc agccgaagat gcggttgcca atcgctggac gcagttcgac ggggatcgcg
     2881 ctgtggtgct cgatggtgag ata
//
LOCUS       DATDMP010000512         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1192696, whole genome shotgun sequence.
ACCESSION   DATDMP010000512 DATDMP010000000
VERSION     DATDMP010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192696"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VI195_08955"
     CDS             complement(<1..440)
                     /locus_tag="VI195_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824557.1"
                     /translation="MSNRALKREEEIYRGNARLATTASIANPLVRLCTDWRFAVRALR
                     YLLGLPVPMDTEDRRVLEQIIFPYLASLADVRRVLFVGCDWYTRHYEPVFFPARTFWT
                     IDPAPRARRFAGRQHIVDALENLPRHFDPDYFDLIICNGVYGFGL"
     gene            complement(437..1597)
                     /locus_tag="VI195_08960"
     CDS             complement(437..1597)
                     /locus_tag="VI195_08960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY6824558.1"
                     /translation="MVRACVVVQNHPNVVMGGAQYQGHLLAEELARRPGVEVTYLARD
                     IPPDAATTFRLPYAVRSIGTWAGIRRRAAFFDAHELWRTLEELQPQVIYQQMRQSYTA
                     ICARYARRAGVPFFFQIASDLDLERRWITNGISANLPFELVETLASIWGLRQAGHIIA
                     QTSRQGLLLRERFGRDPALVVRNFQPLPAQLPGKPPAPLEVLWVANFKEVKRPELFVQ
                     LAESFSGRDDLKFTMVGRPSERQRRFGALMQRIKQTRNLEYLGELSVEAVNRRMAQAA
                     LHVNTSSFEGFPNTFIQAWAQGAVVVTIAVDPDEEGMEKLGIGICAGSFEGLRATIEQ
                     LAHAPQRRLEIAKRAFAFAHANPSLAHGARLADIMLEAAGHSAAARIRAVAP"
     gene            complement(1591..3555)
                     /locus_tag="VI195_08965"
     CDS             complement(1591..3555)
                     /locus_tag="VI195_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEY6824559.1"
                     /translation="MNRLRAMGTREVSYRGLQFMQAQLQRVGLGRTAMIPAGTARGRP
                     WVGTLPQEFPVATYRAAAEQILSGHFRLFGHRDCQLGFPPEWNRDPESGTRAPLAFGK
                     TLNYRDPRIVGNIKYLWETNRHLELVTLAQAWRLTGELRFAHACRELLDSWLRDCPYL
                     LGPNWSSSLELALRLVNWSCAWHLLEGDACILFRDSAGLAFKDRWLNAVRQHCHFVAG
                     HMSLYSSANNHLLGELLGLLVAATTWPCWRESRSWQQQSRARFEEQALLQNGADGVNK
                     EQAIWYQHEVADMMLLAGLSAAHNGCGFGKEFWSRLEAMLTFIASCMDAGGQVPAIGD
                     ADDAVLVRFCPASDLCVYRSLLASGAVLFERADFKYKAGVFDDKSRWLLGDAAAAKFA
                     ALPQQAAPPRRAFEPGGYYILGSDFHAAREVRITVDAAPLGYLAIAAHGHADALSCTL
                     SAAGQQLLIDPGTYCYHTQRRWRDYFRGTSAHNTVRVDGQDQSSSGGSFLWNQHAHAR
                     CLLSEFHADYQRVVCEHDGYRRLHDPVVHRREISYRRPEGRITVSDCVVCAATHYIEI
                     FWHFAEECVLSLRDDVAVATSGMLALTVRWPRELRAELVRGREDPPLGWNSRRFGEKL
                     PTNCLVACGSVGANWQGTTTLDLSWDGAAW"
     gene            complement(3605..>3966)
                     /locus_tag="VI195_08970"
     CDS             complement(3605..>3966)
                     /locus_tag="VI195_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824560.1"
                     /translation="AGLLGAAVATGTFHLLRNVFVWWKVRRDARWLNFAAVAICSILI
                     WGGAVLICLGMKRALSAPPLVAMTAGLAVCSVATLAYVRSPALSSSDRELLRQLFHGR
                     EARLLEKLGVLPRLKHA"
BASE COUNT          746 a         1309 c         1238 g          673 t
ORIGIN      
        1 aggccgaagc cgtagacacc attgcagatg atgagatcga agtagtcggg gtcgaagtga
       61 cgcggcaggt tctcgagcgc atccacgatg tgctggcggc cggcgaatct gcgcgcccgg
      121 ggcgcagggt cgatcgtcca gaacgtgcgc gccgggaaaa ataccggctc atagtgccgc
      181 gtgtaccagt cgcagccgac gaatagcacg cggcgcacat cggcaaggga cgcaagatag
      241 gggaagatga tttgctcgag tacccgacgg tcctcggtat ccatcgggac cggcaggccg
      301 agcagataac gcagcgcgcg caccgcgaaa cgccaatcgg tacacaggcg aaccaacgga
      361 ttggcaatcg acgccgtcgt cgccagacgg gcattgcccc gatagatttc ctcttcgcgc
      421 tttagtgccc tgttactcac ggtgctactg ccctgatgcg agctgcggcg ctatgcccgg
      481 ccgcttccag catgatatcg gcgagtcgcg ccccgtgcgc gagcgacggg ttggcatgcg
      541 caaaggcgaa cgcgcgcttg gcgatctcga gccgccgctg cggagcatgc gccagctgct
      601 cgatagtcgc cctgagacct tcgaacgagc cggcgcaaat gccgatgccg agcttctcca
      661 tgccctcttc gtccggatct actgcaatcg tgacgaccac tgcaccctgc gcccatgcct
      721 gaatgaaagt attgggaaac ccctcgaagc tgctggtgtt cacgtgcagc gcggcctgcg
      781 ccatgcgccg attcaccgcc tccaccgaca actcgcccag gtattcaaga ttgcgagtct
      841 gcttgatccg ttgcatcagc gcgccaaagc ggcgctggcg ctcggacggc cgtcccacca
      901 tggtgaattt caaatcgtcc ctgccgctga acgactcggc cagctgcacg aacaactccg
      961 gtcgcttgac ctccttgaaa ttggcaaccc acagcacctc gagcggcgca ggcggcttgc
     1021 ccggcagctg cgccggcagt ggctggaaat ttcgcaccac cagggcggga tcccggccga
     1081 agcgctcgcg caacagcagc ccctgacgcg aagtctgcgc aatgatgtga ccggcctgcc
     1141 gcagacccca gatactcgcg agtgtctcca cgagctcgaa gggaagattc gccgatatgc
     1201 cgttggtgat ccaacggcgc tccagatcga ggtcgctggc gatctgaaag aaaaacggca
     1261 cgcctgcccg gcgtgcgtag cgcgcgcaaa tcgcggtgta gctctggcgc atctgctggt
     1321 agatgacctg cggctgcagt tcctcgagcg tacgccacag ctcatgcgca tcgaagaacg
     1381 cggcgcgccg gcggataccc gcccaggtgc caatgctgcg gacggcgtac ggcagcctga
     1441 aggttgtcgc agcatccggc gggatatcgc gggcgagata ggtgacctcc accccgggac
     1501 gccgggcaag ctcctccgcg agcaggtgcc cctggtattg cgcgcctccc atgaccacgt
     1561 tcggatggtt ctgtacgacg acgcaggctc ttaccatgca gcgccatccc atgacagatc
     1621 gagcgtggtc gtgccctgcc aatttgctcc gacacttccg caagcgacca ggcaattggt
     1681 cggcagcttc tcgccgaagc gacgcgagtt ccatccgagc ggcgggtcct cacgtccacg
     1741 taccagctcg gcgcgcagct cgcggggcca acgcactgtc agggccagca ttccgctcgt
     1801 ggctactgca acatcgtcac gcaggctgag cacacactct tcggcaaagt gccagaatat
     1861 ctcgatgtag tgagtcgctg cgcagaccac acagtcgctg accgtgatac ggccctcggg
     1921 gcgccgatag cttatttccc gacgatgcac gaccggatcg tgcaggcgcc ggtagccgtc
     1981 atgttcgcaa accactcgct ggtagtcggc gtggaactcg gatagcagac aacgcgcatg
     2041 ggcgtgttgg ttccacagga aactgccacc tgagctggat tggtcctggc cgtcgacccg
     2101 cacggtgttg tgtgccgatg taccgcgaaa gtaatcgcgc cagcgtcgct gcgtgtggta
     2161 gcagtacgtg cctgggtcga tcagcaattg ctggccggcg gccgacaacg tgcatgacag
     2221 cgcatccgcg tgcccgtgtg ccgcgatggc gaggtaccca aggggtgcgg catccaccgt
     2281 tatgcgcact tcacgcgcgg catgaaaatc gctgccgaga atgtagtagc cacccggctc
     2341 gaaagcacgc cgcggcggcg cggcttgctg cggaagtgcc gcgaactttg cggcggcggc
     2401 atcgccgagc agccagcggc tcttgtcgtc gaacaccccg gccttgtatt tgaagtccgc
     2461 tctttcgaac agcacggctc cgctagcgag cagcgagcga tacacgcaca agtcgcttgc
     2521 cgggcaaaag cgcaccagca ccgcatcgtc cgcatctcct atcgcaggca cctgcccgcc
     2581 tgcgtccatg cagctcgcga taaaggtgag catggcctcc aggcggctcc agaactcctt
     2641 gccgaagccg cagccgttgt gcgcggcgct gagaccggcg agcagcatca tgtcggccac
     2701 ttcgtgctga taccagatgg cctgttcctt gttcacaccg tcggcgccat tctgcagcag
     2761 cgcttgctct tcgaaccgtg cgcgcgactg ctgttgccac gagcgacttt cccgccaaca
     2821 gggccaggtg gtcgcggcga ccagtagccc gagcagctcg ccaagcagat gattgttggc
     2881 ggaggaatac agcgacatgt gtccggctac gaaatggcag tgctgcctga cggcgtttaa
     2941 ccagcgatcc ttgaaggcga gaccggcgga atcccggaac aatatgcagg catcgccctc
     3001 gagcaagtgc caggcgcacg accagtttac cagccgcagc gcgagctcca ggctgctgct
     3061 ccagttaggt ccgagcagat acgggcagtc gcgcagccag gagtcgagca gctccctgca
     3121 agcgtgcgca aagcgcagct cgccggtcag gcgccaggcc tgcgccagcg tcaccagctc
     3181 gaggtgccgg ttggtttccc atagatactt gatgttgccg acgatccgcg ggtcgcgata
     3241 attcaaggtc ttgccaaatg cgagcggtgc acgcgtcccg ctctcgggat cgcgattcca
     3301 ttccggcggg aatccgagct ggcagtcgcg gtggccaaac agtctgaagt gtccgctgag
     3361 tatctgctcg gcggctgcgc ggtaggtcgc caccgggaat tcctgcggca gcgtgccgac
     3421 ccacggccgc ccacgtgccg tgccggccgg gatcatggcg gtgcgaccga gcccgacgcg
     3481 ctgcaactgc gcctgcatga attgcagccc gcggtagctc acctcgcggg tgcccatggc
     3541 acgcagtcga ttcatgctcc agtgcagatt cattgctgtg gcgagtggtt atctctggcg
     3601 tcgctcaggc gtgcttcaga cgcggcagca ccccaagctt ctctaacagg cgtgcttccc
     3661 gtccatggaa gagctgccgc agcagctcgc ggtcagagct cgacagcgcc gggctgcgta
     3721 cgtatgccaa ggtcgccacg ctgcacaccg ccaagccggc ggtcatcgcc accagcggcg
     3781 gcgcgctcag cgcgcgcttc atccccaggc agattagaac cgccccaccc cagatgagga
     3841 tgctgcagat tgcgaccgcg gcgaagttga gccagcgcgc gtcgcggcgc actttccacc
     3901 acacgaacac attgcgcagc aggtgaaagg taccggtcgc caccgcggct ccgagcagcc
     3961 ccgcca
//
LOCUS       DATDMP010000513         3656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1971255, whole genome shotgun sequence.
ACCESSION   DATDMP010000513 DATDMP010000000
VERSION     DATDMP010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971255"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VI195_08975"
     CDS             complement(<1..1044)
                     /locus_tag="VI195_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824561.1"
                     /translation="MRNLRHIFHLGIKEILSLARDPVLLFLIAYCFTFSVYTPAKSAV
                     MDVINASVALVDEDDSPLSRQIRDALLPPLFRPARLLAFSMINEAMNSGQFTFIIDLP
                     RKFQNDISRNATPTAEIIADATAMAQAGRGPGYIGNILNQQALSFWPGRADPNNRELV
                     RLNTRVRFNPNVEQSWFVSINQIINNISVMAIFLCGAAVLREREHGTLEHLLVMPLQP
                     YELMFAKIWANGLVVMLAAMISLFLIVKGALGVPVAGSIPLFALGLFTYLFSVTALGI
                     MLSTMVRSMPQFGLLAFPVFIVMNLLSGGQTPLESMPLWLQKTMQFVPSTHFVSFSQA
                     VLFRDANLSMVWPD"
     gene            complement(1045..>3656)
                     /gene="rbbA"
                     /locus_tag="VI195_08980"
     CDS             complement(1045..>3656)
                     /gene="rbbA"
                     /locus_tag="VI195_08980"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEY6824562.1"
                     /translation="MQGGKARVLGGNIDDAKFRTAVSSRIAYLPQGLGKNLYPTLSLF
                     ENADFFGRLFGQSREERERRITDLFTSTDLISFRERPAGKLSGGMKQKLGLCCSLIHD
                     PDLLILDEPTTGVDPLARRQFWELIARIRVQHPTMSVLVATAYMDEAEGFDWLVAMNA
                     GKVLATGTPDELRRKTNETTLERAFIKLLPEELTRGYKEPQITPYKSSGGPPAIEAKG
                     LTQRFGTFTAVDHVNFRIEAGEIFGFLGSNGCGKTTTMKMLTGLLPPTEGTALLFGKP
                     VKGGDVESRRRVGFMTQAFSLYTELTVRQNLVLHARLFDLPPDKSAKRVAELLTDFGL
                     EHVADTLTESLPLGIRQRLSLAVAVLHEPDMLILDEPTSGVDPVARDGFWDLLIRLSR
                     EKRVTIFLSTHFMNEAERCDRMSMMHAGKVLAQGPPAQLVAERKAKNLEAAFIGYLED
                     AAKDPNARDKLAGSKPTTDVAAAFAGAAPAPTAGAEATHADTAGGRFSLGRVWAFARR
                     EAVELLHDRVRLAFAVLGPILLMTVFGYGISLDVDHLPFAILDSDNTVSSRLYADSFR
                     GSKYFDERPPIYNYQQLNQRLRNGELRLGVEIPSGFERDIQRGRQPEVNALVDGAQPF
                     RGETTRGYVQQTHNLYVNRLRDSRGLPPAATPMKVETRMLFNQAFESIYAMVPGDIML
                     LLILIPSMLTALSVVREKELGSIANFYAAPATRSEFLFGKQLPYLGISLIQFATLVAL
                     AIFMFSVPLKGSFATLILGGVLYVVATTGFGLLLSVFSGSQTAAIFAAAIIAILPAVQ
                     FSGMFVPVSSLSGGAWLAGKIFPSTYFQAISVGTFTKALGLPSLWRNLVALAVIAIVY
                     IGLSVALLRKQED"
BASE COUNT          729 a         1106 c         1196 g          625 t
ORIGIN      
        1 atccggccat accatgctca ggttggcatc gcggaacagc accgcctggg agaaactgac
       61 gaagtgcgtc gaaggcacga actgcatggt cttctgcagc cacagcggca tgctctcgag
      121 cggagtctgg ccgcccgaca acaggttcat gacgatgaag accgggaagg ctagcagtcc
      181 gaactgtggc atcgagcgga ccatggtgga gagcatgatg cccagcgccg tcaccgaaaa
      241 gagatacgta aacaacccca gggcaaacag cggaatggag cccgccaccg gcacgccgag
      301 cgcgcccttg acgatgagga acagcgagat catggccgcc agcatcacga caagcccatt
      361 ggcccagatc ttcgcgaaca tgagctcgta gggctggagc ggcatcacca gcagatgctc
      421 gagcgtaccg tgctcgcgct cgcgcagcac cgcggcaccg cacaggaaga tggccatcac
      481 cgagatattg ttgatgatct gattaataga gacgaaccag ctctgctcca cgttgggatt
      541 gaagcgcacg cgggtgttga gcctcacgag ctcccggttg tttgggtcag cccggccggg
      601 ccagaaggac agagcctgct ggttcaggat gttgccgatg tatccgggtc cgcggccggc
      661 ctgagccatg gcggtcgcat ccgcaatgat ctcggcggtg ggcgtggcgt tcctcgagat
      721 gtcgttctgg aacttgcgcg gcaggtcgat gatgaaggtg aactggcccg agttcatggc
      781 ttcattgatc atcgagaagg ccagcaatcg cgccggccgg aacagcggtg gcagcagcgc
      841 atcgcgtatc tggcgcgata gtggcgaatc gtcctcgtcc accagtgcta ccgaggcgtt
      901 gatcacatcc atgaccgcgc tcttggcggg ggtgtagacg gagaacgtga agcaataggc
      961 gatgaggaac agcagcaccg ggtcgcgcgc gagactgagg atctccttga tcccgaggtg
     1021 aaagatgtga cggagattgc gcatctaatc ttcctgcttg cgcaacaatg ccaccgacaa
     1081 cccgatgtag acgatcgcga tcacggccag ggcgacgaga ttgcgccaca gcgaggggag
     1141 tccgagcgcc ttggtgaagg tgccgacgct gatcgcctgg aagtaggtgc tgggaaagat
     1201 cttgcccgca agccacgcgc cgcccgagag ggaggaaacc ggcacaaaca tcccggagaa
     1261 ctgcaccgca ggaaggatcg cgatgatcgc tgcagcgaaa atcgcggccg tctgactgcc
     1321 cgagaacacc gacagcagca ggccgaaacc ggtggtcgcc accacgtaca gaacgccgcc
     1381 gaggatcagg gtcgcgaagc tacccttgag cggcacgctg aacatgaaga tcgcgagcgc
     1441 taccagggtc gcaaactgga tgagcgatat gccgaggtac ggcagctgct tgccgaacag
     1501 gaactcgctg cgcgtcgcgg gtgcagcata gaagttggca atcgatccca gctccttctc
     1561 gcgcaccacg ctcagggcgg taagcatgct cggaatcagg atcaggagca gcatgatgtc
     1621 cccggggacc atggcgtaga tgctctcgaa ggcctggttg aagagcatgc gcgtctcgac
     1681 cttcatcggt gtcgcggccg gcggcagccc gcgtgagtcg cgcaagcgat tcacatacag
     1741 gttgtgggtc tgctgcacgt agccgcgggt agtctcgccg cggaacggct gtgcgccatc
     1801 gacgagagcg ttcacctccg gctggcggcc gcgctgtatg tcgcgctcga agccggacgg
     1861 aatctccacc ccgaggcgca gctcgccgtt gcgcagccgt tgattgagct gctggtagtt
     1921 gtagatcgga ggccgctcat cgaagtactt ggagcccctg aagctgtcgg catacaggcg
     1981 gctgctgaca gtgttgtcgg aatcgagtat cgcgaagggc agatgatcga cgtccagaga
     2041 gataccgtaa ccgaaaacgg tcatgagcaa gatcggcccg agcacggcaa aggcgaggcg
     2101 cacgcggtcg tgcagcagct ccaccgcttc gcggcgcgca aaggcccaca cgcgcccgag
     2161 actgaaccgc ccgcccgccg tgtcagcgtg agtcgcttcg gcgcctgccg tcggagctgg
     2221 agccgcgcct gcgaacgctg ctgctacgtc tgtggttggc ttcgagccgg ccagtttatc
     2281 cctggcgttt ggatccttgg cggcatcctc gaggtagccg atgaaggccg cctcgaggtt
     2341 tttcgccttg cgctcggcca ccagctgcgc cggcggtccc tgcgcgagca ccttgccggc
     2401 gtgcatcatc gacatgcggt cgcagcgctc cgcctcgttc atgaaatgcg tggacagaaa
     2461 gatcgtgacg cgcttctcgc gcgagagacg gatgaggagg tcccagaagc cgtcgcgggc
     2521 gaccgggtct accccggagg tgggctcatc gagtatcagc atgtccggct cgtgaaggac
     2581 cgccacggcg agcgacagcc gctggcggat gccgagcgga agactttcgg tcagcgtatc
     2641 ggcgacgtgc tccaggccga agtccgtgag cagctccgca acacgtttcg ctgacttgtc
     2701 cggggggaga tcgaagagcc gcgcatgcag caccaggttc tgccgcaccg tgagctcggt
     2761 gtagagcgag aaggcctgcg tcatgaagcc cacgcgccgg cgcgactcga catcgccgcc
     2821 cttcaccggc ttgccgaaca gcaacgccgt accttcggtc ggcggcagaa gcccggtgag
     2881 cattttcatc gtggtcgtct tgccgcaacc attggagccg agaaagccga aaatctcgcc
     2941 ggcctcgatg cggaagttga cgtgatcgac cgcggtaaac gtgccaaagc gctgcgtaag
     3001 ccccttagcc tcgatggccg gaggcccgcc gctcgactta tagggagtga tctgcggctc
     3061 cttgtagccg cgcgtcaatt cctcgggcag cagtttgatg aaggctcgct cgagtgtcgt
     3121 ctcgttggtc ttgcggcgca gctcgtcagg tgttccggtg gcgagaacct tgccggcatt
     3181 catggcgacc agccagtcga atccttccgc ctcgtccatg taagcggtcg ccaccagcac
     3241 gctcatcgtg gggtgttgca cgcggatgcg tgcgatcagc tcccagaact gccgccgtgc
     3301 cagcggatcg acgccggtcg tgggctcgtc gaggatcagc agatccgggt catgaatcaa
     3361 cgagcaacac aggcccagtt tctgcttcat gccgccggag agcttgccgg cggggcgctc
     3421 gcggaacgat atcaggtcgg tgctggtgaa caggtcagtg atgcgccgct cgcgctcctc
     3481 gcgcgactgg ccgaacaggc gcccgaaaaa atctgcgttc tcgaataacg acagcgtcgg
     3541 atagaggttc ttgccaagtc cctgcggcag gtaagcaatc ctggaagaca ccgccgtgcg
     3601 aaacttggcg tcgtcgatgt taccgccgag aacgcgggcc tttccgccct gcatct
//
LOCUS       DATDMP010000514         3453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1305230, whole genome shotgun sequence.
ACCESSION   DATDMP010000514 DATDMP010000000
VERSION     DATDMP010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305230"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1587)
                     /gene="tyrS"
                     /locus_tag="VI195_08985"
     CDS             complement(382..1587)
                     /gene="tyrS"
                     /locus_tag="VI195_08985"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY6824563.1"
                     /translation="MNFEEQLAELERGAEEVLTPADLRKKLARATPLRVKAGFDPTAP
                     DLHLGHTVLLNKMRQFQQLGHEVTFLIGDFTGMIGDPSGRNATRPPLSPEEIQANART
                     YQGQVFKILDRERTRIDFNSRWLSKLSSTDIVKLAAHYTVARMLERDDFAKRYKSGQP
                     ISIHEFLYPLAQGYDSVAMRADVELGGTDQKFNLLVGRALQEAYGQEPQVVLTTPLLE
                     GTDGINKMSKSLGNYIGVTEDSDSMFGKLMSISDELMWRYFELLSFRPLGEITSLRAQ
                     AASGRNPRDIKFELAQEIVARFHDAPAAERALRSFIARVSEKAVPQDLPLKVVPVQGP
                     GVRLANLLKEAGLAGSTSEANRKIEEGAVRIDGTRVSDRALTLKVGAEHVLQVGAKRF
                     ARVKLEPST"
     gene            1751..3160
                     /locus_tag="VI195_08990"
     CDS             1751..3160
                     /locus_tag="VI195_08990"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY6824564.1"
                     /translation="MPLAFKPVAKDCIRRLFSAAFWLQLSACAVVGWTLLHGGVSLAP
                     PGQAATLDAGDSAAAALEAATAPDTARDEADASGTAVASGQDTATGSSAQPPSPAEVA
                     SLVLSTTDVTVTRNDTLDRIFRRLKLNLADLASLRNLPGIRHALDSLRPGEALHFTHH
                     DGELFGLERRLNETQTLKVSRRGGALAADVLQNPVELRTRTIHGAIDSSLFEAVAAAG
                     AHDQTAVALADIFGWDIDFVLDVRSGDTFVVTYDEVWRDGTYLRDGPIEAAEFVNQGR
                     AFRAVRYTDPDGHANYYTPEGRNLHKAFLRAPVEFTRVSSRFNSARYHPILNLIRAHK
                     GVDYAAPIGTPVHAAGDGRIRVAGRLGGYGNCVEIEHSRSIVTVYGHLSRFAHGIREG
                     AHVAQGTVIGYVGMTGLATGPHLHYEYRVNGVFKNPQTVSLPAAAPIDARWHEDFMDR
                     SAPLLATLETPPGPMLVSR"
     gene            3266..>3453
                     /locus_tag="VI195_08995"
     CDS             3266..>3453
                     /locus_tag="VI195_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824565.1"
                     /translation="MDAPDLKAPQHYINRELSFLEFNQRVLDQAFDESVPLLERLRFL
                     CISCSNLDEFFEIRVAGL"
BASE COUNT          580 a         1161 c         1082 g          630 t
ORIGIN      
        1 cgcagcggcg gtggctgggg gaagggggcg gcacagtatg cctaaggcga ccccccgtca
       61 acataagtgc ttcacgcacg aacctaccgc ccccgccgca tcgcggccgg ggctgcaggg
      121 ggcgcgcagt atagccccca aagagaggtt gtcaaagtac cgtggactga gcctgggcgg
      181 ggcctggaag cggcttctgg cccgcgattg aggctcttca agcggcatct tcgccggggc
      241 cccaaaggct cctgcgcgcg gccccagccc tttattccta ttggacctcc cttaatgtgg
      301 gggttgagcc tccgttttcc agactccgag cctcgcaggc cgtatgggct cggggcccga
      361 tccccgggct acctgagcaa atcaggtgct tggctcgagc ttgaccctgg cgaaacgctt
      421 cgcgcccacc tgcagcacgt gctccgcccc cactttgagg gtgagcgcgc ggtcggacac
      481 gcgcgtcccg tcgatgcgta ccgccccctc ctcgatcttg cgattcgcct cggaggtgct
      541 cccggcaagc cccgcttcct tgagcagatt cgcgaggcga acccccgggc cctgcaccgg
      601 gaccaccttc aggggcagat cctgcggcac cgctttctcg ctcacgcggg cgatgaaact
      661 gcgcagtgca cgctcggctg ccggggcatc gtgaaagcgg gcgacgatct cctgcgccag
      721 ctcgaacttg atgtcgcgcg gatttcggcc gctcgccgcc tgcgcccgca aagaggtgat
      781 ctcgccgagg ggacggaacg acagcagctc gaagtagcgc cacatgagct catccgagat
      841 cgacatgagc ttgccgaaca tgctgtctga gtcctcggtc accccgatgt agttaccgag
      901 ggacttcgac atcttgttga tcccgtcggt cccctccagc aggggtgtgg tgagcaccac
      961 ctgcggctcc tgaccgtagg cctcctgcag ggcgcgcccg acgagaagat tgaatttctg
     1021 gtccgtgccg ccgagctcga cgtcggcgcg catcgcgacc gagtcgtagc cctgggccag
     1081 agggtagagg aattcgtgaa tggagatcgg ctgtccggac ttgtagcgct tggcgaagtc
     1141 gtcccgctcc agcatacgtg ccacggtgta gtgcgccgcg agcttcacga tatcggtcga
     1201 gctgagcttc gacagccagc gggaattgaa atcgatgcgc gtgcgctccc ggtcgaggat
     1261 cttgaaaacc tggccctggt aggtacgggc attcgcctgg atctcctccg gcgaaagggg
     1321 tgggcgggtg gcgttgcgcc cgctggggtc cccgatcatc ccggtgaagt caccgatcag
     1381 aaaagtcacc tcgtgcccga gctgctggaa ctgacgcatc ttgttgagca gcacggtgtg
     1441 cccgagatgc aggtcaggag cggtcggatc gaaccccgcc ttgacccgca gcggcgtggc
     1501 tcgtgccagc ttcttccgca ggtccgcggg cgtgagcact tcctcagcgc cacgctcgag
     1561 ctctgctagc tgttcctcga aattcatgtg attctcgtga agcgcatacc gaagtcggat
     1621 cgttcgtcct tgtagcctag tgtggccagg agctgaagct gcgtagttaa catacattga
     1681 cgcaaagctc gtgcctccgg tagggtccgg cctcctaaat catctgcaca acaaaaactc
     1741 gtcctcgtcg atgccactcg ccttcaagcc cgtcgcaaag gactgtattc gccgcctctt
     1801 ctcggcggcc ttctggctgc agttgagcgc ctgcgcggtc gtcggctgga cgctgctgca
     1861 cgggggagtc agtctcgccc cgccggggca ggccgccaca ctggacgccg gtgactctgc
     1921 ggcggcagct ctggaggccg cgaccgctcc cgacaccgct cgcgacgaag ccgacgcatc
     1981 gggcactgcg gtcgcctccg ggcaggacac ggcgaccggg tcttccgcac aacccccgtc
     2041 gcctgccgag gtggcgagcc tcgtcctctc cacgaccgat gtcacggtca cgcgcaacga
     2101 cactctcgac cgcattttcc gccggctgaa gctgaacctg gccgacctcg cctccctgcg
     2161 aaacctgccg ggcatacgcc atgcgctcga tagcctgcgg cccggggaag cgctgcattt
     2221 cacgcaccac gatggagagc tgttcggtct cgagcgccgg ctcaatgaga cgcagactct
     2281 gaaagtctca cgccgcggcg gtgccctcgc ggccgatgtc ctgcaaaacc cggtcgagct
     2341 gcgcacccgt accattcacg gcgccatcga cagttcgctg ttcgaggccg tcgcggcggc
     2401 gggagcccac gatcagaccg ctgtcgcact ggcggatatt tttggctggg acatcgactt
     2461 cgtgctggat gtgaggtccg gagacacctt cgtcgtgacc tatgatgaag tctggcgcga
     2521 cggcacgtac ctgcgcgatg gtccgatcga ggcggcggag ttcgtcaacc aggggcgcgc
     2581 attccgcgcc gtgcgctaca ccgatcccga tggtcacgcc aactactaca cgcccgaggg
     2641 tcgcaacctc cacaaagcct tcctgcgcgc gccggtggaa ttcactcgcg tgagctcgcg
     2701 tttcaactct gctcgctacc acccgatcct caacctgatc cgcgcccaca agggcgtgga
     2761 ctacgccgcc ccgatcggca ctcccgtgca cgcggccggc gatggacgta tccgcgttgc
     2821 cggacgtctg gggggttatg gcaactgtgt cgagatcgag cactcccgca gcatcgtcac
     2881 ggtctacgga cacctgtcac ggtttgccca cggcatccgc gagggagcgc acgtcgcaca
     2941 ggggaccgtg atcgggtacg tgggcatgac cgggctcgcc accgggccgc acctgcacta
     3001 tgagtaccgc gtgaacgggg tgttcaagaa cccgcagacg gtatcgcttc cggctgccgc
     3061 accgatcgat gcgcgctggc atgaggattt catggaccgc tcggctccgc ttctggcaac
     3121 cctcgagaca ccgccgggcc cgatgctagt ctcgcgctga gcagaaccca cgccgggcgc
     3181 gccaagcttc ttcacggcac cgtcgtctcg cgctggtatg ctttgaagga agccgccacg
     3241 cgccggccga cgagtcaacg cttccatgga tgcgcccgat ttaaaggcac cacagcatta
     3301 catcaaccgc gagctctcgt ttctcgagtt caatcagcgg gtgctcgacc aggcgttcga
     3361 tgagtcggtg ccactgctcg agcggctgcg gttcctgtgc atctcgtgca gcaatctcga
     3421 tgagttcttc gagatccgcg tggcggggct caa
//
LOCUS       DATDMP010000515         3938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1192864, whole genome shotgun sequence.
ACCESSION   DATDMP010000515 DATDMP010000000
VERSION     DATDMP010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192864"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VI195_09000"
     CDS             complement(<1..602)
                     /locus_tag="VI195_09000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY6824566.1"
                     /translation="MVTSPPSWAPAHAAGPLVVLALAPLWLICMFDRGLWTPDEPREA
                     DIAWRMSQQNDRTLPQLADAPFLEKPPLSYWMSATGLAVFGGSAAAARAANLLYAVIG
                     AGSVLAIALAMQTQAVAAIIAALVAMSALIALRVSVWLAPDACLLAGCALALLGAWRG
                     YNAAAGSSKARGYALMHFGAAIGFMAKSAPGWLVPALALLA"
     gene            complement(657..1487)
                     /locus_tag="VI195_09005"
     CDS             complement(657..1487)
                     /locus_tag="VI195_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824567.1"
                     /translation="MSIPDSVLAAIIAGTATLSASFVQLRSALLREAAAGRASPAARR
                     RNRLQLIILFAVIGAAGVCGFALSQWLTSGERAAQAVMQRELQERVSEISRTASQLEL
                     TRGGERADIESGVLRRIGNDGIVVMANVSACRPPLASPVLTSTPAPAVNASALVATAG
                     PAAPAANSSQPLGDAAVAVMRSCTEAEASPVTLCATIPADASVTEVALFSRAADSTAP
                     WSASRYLPGQESGEARFTATYSESTPEAGTRQVCQGFQHWSSGHARIVRMVVRYSLPV
                     "
     gene            complement(1584..3113)
                     /gene="glpD"
                     /locus_tag="VI195_09010"
     CDS             complement(1584..3113)
                     /gene="glpD"
                     /locus_tag="VI195_09010"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY6824568.1"
                     /translation="MQDAEAASCDLLVVGGGVNGTGIARDAAGRGLSVILCEQDDLAA
                     HTSSASTGLIHGGLRYLEEYHFALVRKALREREVLLAAAPHITRPLRFVMPHAAHLRP
                     AWMIRAGLFLYDHLAARQRLPGSTVIDLRSHVAGAALQAPYRRGFVYSDGQVDDARLV
                     VLNALDAQMRGARILTHTRCEQLVAQDGAWRALLRSAAAAHTVCARAVVNATGPWASG
                     FLAAALPGRAQHQLRLVKGSHLVVPRLFAHRFAYVFQNEDRRIVFAIPYQQDFTLIGT
                     TDLDFHGNPAEVRIKSTEIGYLCALVNRYFRRQIGAADILWSYSGVRPLLEDESSDPM
                     RVTRDYALELEREPAPLLSVFGGKITTYRRLAEDAVDLLAALLGVRAAPWTAAALLPG
                     GDLPRGSLAAFLRTLARRYPWLPAPLRRRYAHAYGTRIEPLLGAATALSDLGEQLLPQ
                     LYEREVEYLCNEEFAHTVPDILWRRSKLGLHLLQTDPGPLERWLAGHAGRALVSAASP
                     V"
     gene            complement(3141..>3938)
                     /locus_tag="VI195_09015"
     CDS             complement(3141..>3938)
                     /locus_tag="VI195_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824569.1"
                     /translation="LPLGFRETARRNIELCLPELSVAQRKHLLQEHFASLGIALLEIP
                     FAWWCPPERLAKLVRLEGTEHLAAALARGRGAMVLTAHFTSLELAGRALTHVATIGFL
                     YRPPKNEVIAYAFERFRVGFGGHGIPRDDIRAFITALRSNECVWYAPDQSYRKKGAEM
                     VPLFGIPAASNTLTSRLARLTGAPVLPYFFQRLPGTAGYLGVIHPPLDDFPSESSVAD
                     TARFYRMIEAQVRRVPEQYLWIHRRFKGLSADYPDYYRRQAPRQTTS"
BASE COUNT          689 a         1300 c         1387 g          562 t
ORIGIN      
        1 gctagcagtg cgagtgccgg caccagccat cccggggcac tctttgccat gaagccgatg
       61 gcggcgccga aatgcatgag cgcgtaaccg cgtgccttgg aggaacccgc tgcggcgttg
      121 tagccgcgcc aggcgccgag cagcgcgagc gcacacccgg cgagcaggca cgcatcaggc
      181 gcaagccaca cgctgacccg caatgcgatg agcgcgctca tcgcgacgag ggccgcgatg
      241 atggccgcga ccgcctgagt ctgcatcgcc agcgcaatgg cgaggacgct gcccgcgccg
      301 atcaccgcat agaggagatt ggcggctcga gctgcggcgg cggagcctcc gaacaccgca
      361 agtcccgtcg ccgacatcca gtacgagagc ggcggtttct cgaggaacgg cgcatcagcg
      421 agctggggca gcgttcgatc gttctgctga ctcatgcgcc aggcgatgtc ggcttcgcgt
      481 ggctcatcgg gtgtccacag gccacgatcg aacatgcaga tcaaccacag cggcgcaagc
      541 gcgagcacga ccagcggacc cgccgcgtgc gcaggcgccc acgaaggtgg cgatgtgacc
      601 aacagctccc ctcggctcga tcttctcgtg aggcgattat gcgctactgg ttgaggtcag
      661 acgggcaggg agtagcgcac caccatgcgc acgatgcggg cgtgaccgct cgaccagtgc
      721 tggaaaccct gacagacctg ccgtgtgcca gcctcggggg tgctctcgga gtaggttgcg
      781 gtaaagcggg cttccccgga ttcctgtccc ggaagatagc gactcgcgct ccagggggcg
      841 gtggagtcgg cagcgcggct gaagagcgca acttcggtga cgctcgcatc ggcggggatg
      901 gttgcgcaaa gcgttaccgg gctcgcctcg gcttcggtgc aggagcgcat gacggcgaca
      961 gccgcatcgc ccagaggctg cgagctgttg gccgccggcg cggccggccc ggcagtcgcg
     1021 accagagctg acgcgttgac ggcgggcgcc ggcgtgctcg tcagcaccgg cgacgcgagc
     1081 ggcggacggc atgccgatac gttggccatc accacgatgc catcgttacc gatccgccgc
     1141 agcacgccgc tctcgatgtc ggcgcgctcg ccgccgcgcg tgagctcgag ctgactcgcg
     1201 gtgcggctga tctcggagac gcgctcctgc agttcgcgct gcatcacggc ctgcgcggcg
     1261 cgctcgcccg atgtgagcca ctgcgagagc gcgaagccgc acacaccggc ggcaccgatg
     1321 actgcaaaca ggatgatgag ctgcagccgg ttgcggcgac gcgcagcggg cgaagcgcgg
     1381 cctgccgccg cctcgcgcag caacgccgag cgcagttgca cgaacgacgc cgacagcgtc
     1441 gcggtacccg cgatgatggc ggcgagcact gagtcgggta tcgacatggg cgcttccgcg
     1501 gcaagctcgt cccgccacgc tagcacggcc ggtcgaccga tagcctctcg gcgggcgccc
     1561 tgatccgaaa aacgcgatgc acatcacacg gggctcgcgg cgctcaccag tgcgcggccg
     1621 gcgtgcccgg caagccagcg ctcgagcgga ccgggatcgg tctgcagcag atgcagaccg
     1681 agcttcgaac gccgccagag gatgtcaggc accgtgtgcg cgaactcctc attgcacagg
     1741 tactcgactt cgcgctcgta gagctgcggc agcaactgct cgccaaggtc gctgagtgcc
     1801 gtggcggcgc ccagtaatgg ctcgatgcgc gtcccgtatg cgtgcgcgta gcggcgccgc
     1861 agaggcgcgg gcagccaggg atagcgtcgc gcgagcgtgc gcaggaacgc ggccaggctg
     1921 ccccgcggca ggtccccgcc cggcagcaac gctgcggcgg tccagggtgc cgcgcgcaca
     1981 ccgagcagcg cggcgagcag gtcgacggca tcctccgcca gacggcgata ggtggtgatc
     2041 ttgccgccaa acaccgagag cagcggcgcg ggttcgcgct cgagctcgag cgcatagtcg
     2101 cgcgtcacgc gcatcggatc gctcgactcg tcctcgagca gcggacgcac gccggagtag
     2161 ctccacagta tgtctgccgc gccgatctgc cggcgaaagt agcgattgac gagcgcacag
     2221 aggtagccga tctcggttga cttgatgcgc acctcggcag gattgccgtg aaaatccagg
     2281 tcggtcgtgc cgatgagagt gaagtcctgc tggtagggga ttgcgaagac gatgcgccgg
     2341 tcctcgttct ggaaaacgta cgcgaagcgg tgcgcgaaca gtcgcggcac caccagatga
     2401 ctacccttga ccaggcgcag ctgatgctgc gcacgccccg gcagggccgc ggcgaggaaa
     2461 ccactagccc acgggccggt ggcgttgacg acggcgcggg cgcagacggt gtgggctgcc
     2521 gccgcgctgc gcagcagcgc ccgccaggcg ccatcctggg caacaagctg ctcgcaacgg
     2581 gtatgcgtca ggattctggc accgcgcatc tgggcatcga gcgcgttgag caccacgagc
     2641 cgtgcgtcat cgacctgtcc gtcggaatac acgaaaccgc gccgataggg cgcctgcagc
     2701 gcggcgccgg ccacatggct acgcaggtcg atgaccgtcg agcctggcag gcgctggcgt
     2761 gcggccaaat ggtcatagag gaagagtccc gcgcggatca tccacgccgg gcgcagatgc
     2821 gccgcgtgcg gcatcacgaa gcgcaacggc cgcgtgatgt gcggcgcggc cgccagcagc
     2881 acttcgcgct cccgcagcgc cttgcgtacc aacgcgaagt gatactcctc gagatagcgc
     2941 agtccgccgt ggatcagccc ggtgctggcg gaagaggtgt gcgccgccag atcgtcctgc
     3001 tcgcacagga tgaccgagag cccacggccg gcggcgtcgc gggcgatgcc ggtgccgttc
     3061 acaccgccac cgaccaccag caggtcgcag cttgcggcct cagcgtcctg catcgccgcc
     3121 gtgaattact gctgtggctc tcatgaggtg gtttgccgcg gtgcctgccg tcggtagtag
     3181 tccggatagt ccgcggagag ccccttgaag cggcggtgaa tccacaggta ctgctcaggg
     3241 acgcgccgta cctgtgcctc gatcatacgg tagaaacgtg cggtgtcagc gacgctggac
     3301 tcgctcggaa agtcatcgag cgggggatgg atcactccga ggtagcccgc ggtacccggc
     3361 agccgctgga agaaataagg cagcacgggt gcgccggtca ggcgcgccag gcgcgaagtg
     3421 agcgtgttgc tcgctgcggg gatgccgaac agcggcacca tctccgcgcc cttcttgcga
     3481 tagctctgat ccggcgcgta ccagacgcat tcattgcttc tcaaggcggt gatgaaggcg
     3541 cggatgtcgt cacgcggaat gccatgcccg ccaaaaccga cacgaaagcg ctcaaaggca
     3601 taggcgatga cctcgttctt tggcggccgg tacaggaagc cgatggtggc cacatgcgtg
     3661 agcgcgcggc ccgccagctc gagcgaagtg aagtgtgccg tgagcaccat ggcgccgcgc
     3721 ccgcgcgcga gcgccgccgc gaggtgctcg gtgccctcga gacgcacgag cttcgcgagc
     3781 cgctcgggcg ggcaccacca tgcgaaaggg atctcgagca gcgcgatacc gaggctggca
     3841 aagtgctcct gcagcaggtg tttgcgttgc gcgaccgaga gctccggcag gcacagctcg
     3901 atgttgcgtc gtgcggtttc ccggaagccg agcggcag
//
LOCUS       DATDMP010000516         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1526336, whole genome shotgun sequence.
ACCESSION   DATDMP010000516 DATDMP010000000
VERSION     DATDMP010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526336"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..678
                     /gene="rpsD"
                     /locus_tag="VI195_09020"
     CDS             55..678
                     /gene="rpsD"
                     /locus_tag="VI195_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY6824570.1"
                     /translation="MARYTGPKCKLARREGTDLFLKSGVKPLENKCKLTVPPGGLKSE
                     RRQRLSDYGLQLREKQKLRRMYGVLERQFGNYYEEAARKSGATGENLLRLLECRLDNV
                     VYRMGFAATRAEARQLVSHKAISVNEKPVSIASYQVKAGDIVQVREKARKQLRIQSAL
                     NVAQQVGLPEWVEVNDKEFRGVFKSAPGRDDVLPDINENLVVELYSK"
     gene            777..1781
                     /gene="rpoA"
                     /locus_tag="VI195_09025"
     CDS             777..1781
                     /gene="rpoA"
                     /locus_tag="VI195_09025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY6824571.1"
                     /translation="MQGSVSEFLRPRVVKVQPIAPRQARVVIEPFERGFGHTLGNALR
                     RVLLSSMPGSAITEVEIDGVLHEYTSIEGVQEDVVDILLNLKSVALRMHARDSAELRL
                     HKKGPGPVTAGDIQVDHDIEVVNPELVIANLTKAGELSMTLRVERGRGYRPAASRLAF
                     EEQTRPIGRLQLDASFSPVRRVTYSVDAARVEQRTDLDKLVIDIETNGTIDAEEAIRR
                     AGSILKDQLSVFVDLQGEEEAATKVSEMQFDPILLRPVDELELTVRSANCLKAENIHY
                     IGDLVQRTEVELLRTPNLGKKSLTEIKEVLQSHGLMLGMRLDGWPPAGLKHDEERDVI
                     "
     gene            1792..>2215
                     /gene="rplQ"
                     /locus_tag="VI195_09030"
     CDS             1792..>2215
                     /gene="rplQ"
                     /locus_tag="VI195_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY6824572.1"
                     /translation="MRHHLSGRQLSRNSSHRHALMRNMSVSLLRHETIRTTLPKAKEL
                     RRVVEPLITLAKSDGEANRRVAFSRLRDAEVVEKLFADLGPRFKARPGGYTRILRMVP
                     RPGDSAPMALMQLVEGATPVAAEEPTEAKKKRSRRAKAP"
BASE COUNT          409 a          707 c          751 g          348 t
ORIGIN      
        1 acaacggatg ccgtccgccc aagaagcgcc gcgtgtagag acaacgagga aaccatggcg
       61 cgttacaccg gaccgaagtg caagctcgct cgccgcgagg gcaccgacct gttcctgaag
      121 agcggcgtta agccgctgga aaacaagtgc aagctcacgg tgccgccggg cggcctcaag
      181 agtgagcggc ggcagcgcct gtccgactac gggctgcagc tgcgcgagaa gcagaagctg
      241 cggcgcatgt atggggtgct cgagcgtcag ttcggcaact attacgagga ggccgcgcgc
      301 aaatccgggg ccaccggcga gaacctgctg cgcctgctcg agtgccgcct ggacaacgtg
      361 gtgtaccgca tgggctttgc ggcgacgcgc gccgaagcgc gccagctggt gagccacaag
      421 gctatcagcg tcaatgagaa gcccgtcagc atcgcctcct accaggtgaa ggcgggcgac
      481 atcgtgcagg tgcgcgaaaa ggcgcgcaaa cagctgcgca tccagagcgc cctgaacgtg
      541 gcccagcagg tcgggctgcc cgagtgggtc gaggtgaatg acaaggagtt ccgcggcgtg
      601 ttcaagtcgg cgccgggccg ggacgacgtc ctgcccgaca tcaacgagaa cctggtcgtc
      661 gagctgtatt cgaagtaatc gcatccgctt gaatttgcgg ggctgtgccc ccatacgagc
      721 gggttcgccc gtgttccttt tgcgccggcc ctgtgagccg gcaaggtgag actgcaatgc
      781 agggttcagt gagcgagttt ttgcgacccc gtgtggtgaa ggtgcagccg atcgctccgc
      841 gccaggcgcg cgtggtgatc gagccgttcg agcgcggctt cggccacact ctcggcaacg
      901 cgctgcgccg cgtgctgttg tcctcgatgc ccgggagcgc catcaccgag gtcgagatcg
      961 atggcgtgct gcacgagtac accagcatcg agggggtgca ggaggatgtg gtcgatatcc
     1021 tgctgaatct caagtcggtg gcgctgcgca tgcatgcgcg cgactctgcc gagctgcggc
     1081 tgcacaagaa gggcccaggc ccggtgactg ccggtgacat tcaggtcgat cacgacatcg
     1141 aggtagtgaa ccccgagctg gtgatcgcca atctcaccaa ggcgggggag ctgtcgatga
     1201 ccctgcgcgt cgagcgcggc cgcggttatc gcccggcggc ctcgcgcctc gcgttcgagg
     1261 agcagacgcg ccccatcgga cggctgcagc tcgatgcctc cttctcgcca gtgcggcgcg
     1321 tcacctactc ggtcgacgcg gcgcgcgtcg agcagcgcac cgacctcgac aaactggtga
     1381 tcgatatcga gaccaacggc acgatcgatg cggaggaggc gatccgccgt gccggcagca
     1441 tcctcaaaga ccagctgtcg gtgttcgtcg acctgcaggg tgaggaggag gccgccacca
     1501 aggtctcgga gatgcagttc gatccgatcc tgctgcgtcc cgtggacgag ctcgagctca
     1561 ccgtgcgcag cgccaactgc ctcaaggccg agaacatcca ctacatcggc gatctggtgc
     1621 agcggaccga ggtcgagctg ctgcgcacgc cgaacctcgg caagaagtcg ctgaccgaga
     1681 tcaaggaagt gctgcagagt catgggctga tgctcggcat gcgcctcgac ggctggccgc
     1741 cggccggtct caagcacgac gaagagcgcg acgtcatctg aaggttcgtt tatgcgtcac
     1801 catctctccg gccgccagct ctcgcgtaac agctcgcatc gccacgcgct gatgcgcaat
     1861 atgtcggtgt cgctgctgcg gcacgagacc atccgcacca cgctccccaa agccaaggag
     1921 ctgcggcgcg tggtcgagcc gctgatcaca ctcgccaaga gtgacggcga ggccaaccgc
     1981 cgcgtcgcct tctcacgctt gcgtgatgcg gaggtggtcg agaagctgtt cgccgacctg
     2041 ggtccgcgtt tcaaggcgcg gcccggtggc tacacgcgca ttctgcgcat ggtgccccgt
     2101 cccggcgact cagcccccat ggcgctcatg caactcgtcg agggcgcgac cccggtagcg
     2161 gccgaggagc cgacggaggc gaagaagaag cgctcgcggc gagccaaagc gccag
//
LOCUS       DATDMP010000517         2063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_194452, whole genome shotgun sequence.
ACCESSION   DATDMP010000517 DATDMP010000000
VERSION     DATDMP010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194452"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VI195_09035"
     CDS             complement(<1..685)
                     /locus_tag="VI195_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY6824573.1"
                     /translation="MRMDKLTSRFQQALAEAQSLALGRDHQFMEPAHVMLALLDSSGG
                     SVRPLLMKAGADVNKLRAGVLALLDSLPKVEGTPGDIHISNDLNRILNVTDKLAQQRG
                     DQYISSELFVLAAFEDRALARLFKEAGVVRGAVEKAIDEVRGCEKVNDANAEESRQAL
                     EKYTIDLTARAASGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIAEGL
                     AQRVVSGDVP"
     gene            complement(838..1602)
                     /locus_tag="VI195_09040"
     CDS             complement(838..1602)
                     /locus_tag="VI195_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824574.1"
                     /translation="MNSDFFPFVDLTAPRMRFMHREANELPGLTMLSIPVLDLLRSGS
                     ASGPTLEPLRHSALARDARVRRALGIRQALLNGRLDGLDAISAAHVMLLRTSAAQCTD
                     RQAQETWKRAARDVGAMTASYLSPTELADVWSSVRSSPCYRDTSGPHRDWADLLAAVA
                     ARNAAEMVRIGAPLLETDSSLSKEEQTYLTTVLATGYVHLGELPQARTLLVSRWSQLD
                     HGGEFALSLNQLLALAVDGQPALAGSRADDAGAHGS"
     gene            complement(1611..>2063)
                     /locus_tag="VI195_09045"
     CDS             complement(1611..>2063)
                     /locus_tag="VI195_09045"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6824575.1"
                     /translation="SEYLGLIHGVFPRARIIYLQRDALDTCLSCYFQQFSPQMNYATD
                     LANLAHYYREHRRLMAHWRNVLPPQSLLEVPYAELIADQEKWTRKIVDFVGLPWDARC
                     LEFYTTERTVNTASVWQVRQKIYASSIGRWRNYQKFIGPLLELRDATP"
BASE COUNT          334 a          668 c          705 g          356 t
ORIGIN      
        1 ccggcacgtc tccgctcacg acccgctggg ccagcccctc ggcgatcgcg gtcttgccga
       61 cgccgggttc cccgatcagc acggggttgt tcttggtgcg ccgctgcagc acctggatcg
      121 cgcggcggat ctcgtcgtcg cgcccgatga ccgggtcgag cttgccggag gcggcgcgcg
      181 cggtgaggtc gatggtgtat ttctcgagcg cctggcggct ctcctcggcg ttggcatcat
      241 tgactttctc gcagccgcgc acctcatcga tcgccttctc gacggcgccg cgcaccaccc
      301 ccgcctcctt gaagagccgc gccagcgcgc gatcctcgaa ggcggccagc acgaacagct
      361 cgctcgagat gtactgatcg ccgcgctgct gcgcgagctt gtcggtgacg ttgaggatgc
      421 ggttgaggtc gttggagatg tggatatccc ccggagtgcc ctcgaccttc ggcagtgaat
      481 cgagcagtgc caggacgccg gcacgcagct tgttgacgtc ggcacccgcc ttcatcagca
      541 gcggacgcac cgagccgccc gagctgtcga gcagcgccag catgacgtgg gcgggctcca
      601 tgaactggtg gtcgcgcccg agagccagcg actgggcctc ggccagcgcc tgctgaaaac
      661 ggctggtgag tttgtccatg cgcatgggga tcggcctcag acggggatga tcgctttgtg
      721 gggataaaca gcagccgctt caagaggctg cgacgccggc gccggttcga gaagtgtatt
      781 ccgccggcag accccacgca tcccgcgcgg gaggctgcgc ggccttgggg aaacgctcta
      841 ggagccgtgg gcgcctgcgt cgtcggctcg gctgcccgcc agtgccggct ggccgtccac
      901 cgccagcgcc agcagctggt tgagcgagag ggcgaactct ccgccatggt cgagctggct
      961 ccagcgcgag accagcaggg tgcgtgcttg cggcaactcc cccaggtgga catagcctgt
     1021 ggcgagcacc gtggtcagat aggtttgctc ctcctttgaa agcgaggagt cggtctccag
     1081 caacggcgcg ccgattctca ccatctctgc ggcgttgcgt gccgccaccg ctgcgagcag
     1141 atcggcccag tccctgtggg ggccgctggt gtcccgatag cagggcgagg acctgacact
     1201 gctccagaca tcggccagct ctgtgggact caggtaggag gcggtcatcg cgccgacatc
     1261 gcgggccgcc ctcttccagg tttcctgcgc ctgacggtcg gtgcactgcg ccgcgctcgt
     1321 gcgcagcagc atgacatggg ccgcgcttat cgcatcgagg ccgtccagcc ggccatttag
     1381 cagcgcctga cgtatgccga gggcgcgccg cacgcgcgca tcgcgagcga gtgcgctatg
     1441 ccgcaagggc tcgagcgtgg gaccggacgc cgagccgcta cgcagcagat ccagcacagg
     1501 gatggacagc atcgtgagcc cgggaagctc gttcgcctca cgatgcatga aacgcatgcg
     1561 cggcgcagtg agatccacga acgggaagaa gtctgagttc accggagccg ctacggtgtg
     1621 gcgtccctca gctcgagcag cgggccgatg aacttctggt agttgcgcca gcgaccgatg
     1681 gagctggcgt agatcttctg ccgaacctgc cagacgctgg cggtgtttac ggttcgttcg
     1741 gtcgtgtaga actccaggca gcgcgcatcc cagggcagcc ccacgaaatc cacgatcttc
     1801 cgcgtccatt tctcctggtc cgcgatgagc tccgcgtagg gtacttcgag caggctctgc
     1861 ggcggcaata cgttgcgcca gtgcgccatc aaccgccggt gctcgcggta gtaatgcgcc
     1921 agattcgcga gatcggtagc gtagttcatc tggggcgaga actgctggaa gtagcaggac
     1981 agacaggtat cgagcgcgtc ccgctgcaga tagatgatgc gggcgcgtgg gaagacgcca
     2041 tggatcaggc ccaggtactc gga
//
LOCUS       DATDMP010000518         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_750632, whole genome shotgun sequence.
ACCESSION   DATDMP010000518 DATDMP010000000
VERSION     DATDMP010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750632"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VI195_09050"
     CDS             complement(<1..1184)
                     /locus_tag="VI195_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6824576.1"
                     /translation="MKDLTQGPISRLLVAMAVPIAIGMLFQTLYFLVDLYFVSRLGDA
                     AIAGVSTAGTVSFVILALTQMLGVGTVALVSHAAGRKDQAQANLVFNQSVLLSAVCGT
                     LTLLWGLLAGGAYVRWVAADAAAAAAGTAYLYWYTPGLALQFALVAMGSALRGTGIVQ
                     PTMLVQMLTVILNTVLAPILIAGWGTHHPFGTAGAGLASSIAIACGVIFLWVYFVRLE
                     HYVALDRHEWRPRWALIGRMLNIGLPAGGEFLMMFVIIGIIYLVIRSFGPAAQAGFGI
                     GGQVMRALFLPMMAIAFAATPVAGQNFGAGRFDRVRETFRKAALLSGVLMLLITLLCQ
                     WHPEWFIHAFTRAPEVVAVGAPYLRITSWNFIATGLIFTCSGLFQALGNTWPSLLSSA
                     SRL"
     gene            complement(1181..2269)
                     /locus_tag="VI195_09055"
     CDS             complement(1181..2269)
                     /locus_tag="VI195_09055"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEY6824577.1"
                     /translation="MDVRAATRDYETWLERAVPVVRADLQRKHRLMAQDAFSFLRATF
                     YRWSQLWPEACHELVDAPAVLAVGDLHIENFGTWRDAEGRLIWGVNDFDETVQLPYTQ
                     DLVRLGTSALLAIEESRLCLSARRACAAILEGYAQSMHERGDTVVLAERYRWLRDLAV
                     ARLKDQRPFWQHLRSLRAPPGRIPAPVRQLLQRALPAGAGEVRLVHRQAGLGSLGRLR
                     LVALAKWHGAMVAREAKPLTPSAWLWARGARLRTTRYGEIIARALRVPDPCLALHGEW
                     LIRRLAPDCSRIELSSLPRSRDEDRLLWMMGWETANMHLASAGQRRRILADLRARSGR
                     WLGKAAGRMAQAVERDWRRWRKGALLKP"
     gene            complement(2308..>2980)
                     /locus_tag="VI195_09060"
     CDS             complement(2308..>2980)
                     /locus_tag="VI195_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HEY6824578.1"
                     /translation="PFHELRIYRPQTPAQQLALLLSGDGGWGAPVAEIATRLSAEGTL
                     VAGIDVRDLFARYEREAQGCASPGEDLEQLAHYLQQRYALPAAAPVLIGHSAGATLAY
                     LALARSAPGTFAGALTLSFCADLDLLKPLCAAPPPGSLRRSDGIRLLPTAAALPAPWF
                     ALHGLDDVECPAQEAREFAAAIPEAHFIALPGITHSYHHMGRWWPVFDGAWRQLVAAP
                     GAGAP"
BASE COUNT          541 a         1016 c         1035 g          388 t
ORIGIN      
        1 aggcgcgatg ccgagctcag tagcgacggc caggtattgc cgagcgcctg aaacagcccc
       61 gagcaggtga agatcaggcc ggtagcgatg aagttccagg aagtgatgcg caggtatggc
      121 gcgcccaccg ccaccacctc cggtgcgcgc gtgaaggcgt ggatgaacca ctcggggtgc
      181 cactggcaga gcagcgtgat cagcagcatg agcacgccgc tcagcagtgc cgccttgcgg
      241 aaggtctcgc gcacgcgatc gaaccgcccc gcgccgaagt tctgtccggc aaccggcgtc
      301 gccgcgaagg cgatggccat catcggcagg aacagcgccc gcatgacctg cccgccgatg
      361 ccgaatccgg cctgggccgc ggggccgaag gagcggatca ccaggtagat gattccgatg
      421 atcacgaaca tcatcaggaa ctcaccgccg gcgggcaggc cgatattgag catccgtccg
      481 atgagcgccc agcgcggccg ccactcgtgt cgatcgagcg ccacgtagtg ctcgagccgt
      541 acgaaataca cccacaggaa gatcacaccg caggcgatgg cgatactgct cgcaagtcct
      601 gcgcccgcgg ttccgaaagg atggtgcgtg ccccagccgg cgatgagaat cggcgcgagc
      661 acggtgttca ggatcaccgt cagcatctgc acgagcatgg tcggctgcac gatgccggtg
      721 ccgcgcaacg ccgagcccat cgctacgagc gcgaactgca gcgccagccc cggggtgtac
      781 cagtacagat aggccgtgcc cgcggccgcg gcggcggcat cggcggccac ccagcgcacg
      841 taagccccac cggcgagcag cccccagagg agcgtgagcg tgccgcacac cgccgagagc
      901 agcaccgact gattgaacac caggttggct tgcgcctgat ccttgcgccc ggcggcgtgg
      961 gacacgagcg ccaccgtgcc cacaccgagc atctgcgtga gcgcgaggat cacgaagctc
     1021 acggtgccag cggtgctcac cccggcgatg gcggcatcgc cgaggcgtga gacgaagtag
     1081 aggtcgacga ggaagtacag cgtctgaaag agcatgccga tggcgatcgg caccgccatc
     1141 gccacgagca ggcggctgat cgggccctgg gtgaggtctt tcatggcttc agcaacgctc
     1201 ctttacgcca tcgtcgccag tcacgctcga ccgcctgtgc catgcggccg gcggccttgc
     1261 cgagccaacg cccactgcgc gcgcgcagat ccgcgaggat acgccgccgc tggcccgcac
     1321 tcgcgagatg catgttggcc gtctcccagc ccatcatcca cagcagccgg tcctcgtcac
     1381 ggctgcgcgg caggctggag agctcgatgc ggctgcagtc gggtgcgagg cgccggatca
     1441 accactcacc gtgcagagcg agacaggggt cgggcacacg cagcgcccga gcgatgatct
     1501 cgccgtagcg tgtcgtgcgc agccgagcgc cccgcgccca gagccaggcc gagggcgtta
     1561 gcggtttcgc ctcgcgtgcg accatggcgc catgccactt tgcgagcgcc acgagacgca
     1621 gccgcccgag gctgccgagc cccgcctgcc ggtgcaccag gcgtacttcg cccgcgccgg
     1681 ccggtagcgc ccgctgcagc agttgccgta cgggcgccgg gatgcgtccg ggcggtgcgc
     1741 gcagcgagcg caggtgctgc cagaaaggac gctggtcctt gagacgcgcc accgcgaggt
     1801 cccgcagcca gcgatagcgc tcggccagca ccacggtgtc accgcgctcg tgcatcgact
     1861 gggcgtagcc ctcgaggatc gcggcgcagg cgcggcgtgc agaaagacac agacgcgact
     1921 cctcgatcgc cagcagcgcg ctcgtgccga ggcgcaccag gtcctgggtg tagggcaact
     1981 gcaccgtctc atcgaaatcg ttgacgcccc agatgagccg tccttccgca tcgcgccagg
     2041 ttccgaagtt ctcgatgtgc aggtcaccga ccgcgagcac tgccggggca tcgacgagct
     2101 cgtggcacgc ttcgggccag agctgcgacc agcggtagaa ggtggcacgc aggaacgaga
     2161 aggcgtcctg tgccatgagc cggtgcttgc gctggaggtc tgcgcgcacc actggaaccg
     2221 cgcgctcgag ccaggtctca tagtcacggg tggccgcgcg cacatccatg gatcacacct
     2281 taatggagtg cggcggacac ggccacgtca gggagcgccc gcgcccgggg ctgcgactag
     2341 ctgacgccag gctccatcga acaccggcca ccagcggccc atgtggtgat agctgtgggt
     2401 gatgcccggc agcgcgatga aatgcgcctc ggggatcgcg gcggcaaact cgcgcgcctc
     2461 ctgcgccgga cactccacgt catcgagtcc atgcaaggcg aaccagggag ccggcagagc
     2521 cgctgcagtg ggcagcagcc gtatgccgtc gctgcgccgc agagagccag gaggcggcgc
     2581 ggcacacaat ggcttcaaga gatcgaggtc ggcgcagaag gatagcgtga gcgcgccggc
     2641 gaaggtaccc ggcgcggagc gcgcgagcgc gagataggcg agagtcgcgc cggccgagtg
     2701 cccgatgagc accggcgcgg cagcgggcag cgcatagcgc tgctgcaggt agtgcgcaag
     2761 ctgctcgaga tcctcaccgg gtgaggcgca gccctgcgcc tcgcgctcgt agcgcgcaaa
     2821 gagatcgcgc acatcaatgc ccgccaccag cgtcccctcg gcactcagac gcgtggcgat
     2881 ctcggcaacc ggtgcgcccc agccgccatc gccggagagg agcagcgcga gctgctgcgc
     2941 gggagtctgt gggcggtaga tgcgcagctc gtggaatggc
//
LOCUS       DATDMP010000519         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_194767, whole genome shotgun sequence.
ACCESSION   DATDMP010000519 DATDMP010000000
VERSION     DATDMP010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194767"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VI195_09065"
     CDS             complement(<1..463)
                     /locus_tag="VI195_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY6824579.1"
                     /translation="MTRRRVVVTGLGIVSPIGNSVSEAWQNCLAGRSGITRITRFDAS
                     RLASQIAGEVKGFDVAPYMSPKEARRMNLFIHYGMAAGLQAWRDSGLQVTPENAERVG
                     VNFGSGIGGLPLIEQMHDELGKNGPRRISPFFIPGSIINMIAGLLSIEVGAK"
     gene            complement(464..703)
                     /gene="acpP"
                     /locus_tag="VI195_09070"
     CDS             complement(464..703)
                     /gene="acpP"
                     /locus_tag="VI195_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY6824580.1"
                     /translation="MENIEQAVKKIIAEQLGVNEAEIKNESSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAIDYIKAHAKS"
     gene            complement(734..1453)
                     /gene="fabG"
                     /locus_tag="VI195_09075"
     CDS             complement(734..1453)
                     /gene="fabG"
                     /locus_tag="VI195_09075"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY6824581.1"
                     /translation="MTMLEGKVALVTGASRGIGQAIAQELARLGAKVVGTSTSEAGAA
                     KVPGSGRVLDVRNAAQCDALVEDIQKQVGDIAILVNNAGITRDNLALRMKDGDWDEVM
                     ETNLRAVFLLSRAVMRGMMKARWGRIVNITSVVGAAGNPGQANYAAAKAGVVGMTKSL
                     ARELGSRNITVNCIAPGFIDTDMTRALSDEQRNALLAQIPLGRLGSAADVAAAVGYLV
                     SPGAGYVTGAVLHVNGGMYMS"
     gene            complement(1450..2376)
                     /gene="fabD"
                     /locus_tag="VI195_09080"
     CDS             complement(1450..2376)
                     /gene="fabD"
                     /locus_tag="VI195_09080"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY6824582.1"
                     /translation="MKLAMVFPGQGSQSVGMLRGYDGLPEIDEVRADARAVLGAEFVR
                     LLDEGPADDLNLTVNTQPAMVTAGYAVYRAWRALGGPAPEVMAGHSLGEYTALTAAGA
                     IGFKDCLPLVRFRAQAMQEAVPAGQGAMAAILGMDDQAARAACAEADGEVQAVNFNAP
                     GQVVIAGEKAAVERAIEKCKAHGAKRAMALPVSAPFHSTLMRPAAQRLQVYLAKVTLA
                     APECAVVNNVDVQMESEPSRIRDALVRQAASPVRWVETIQAMAAMGATHIVECGPGKV
                     LAGMTKRIAPQVQSFTLSDRAAVEQALGAMKG"
     gene            complement(2373..>2725)
                     /locus_tag="VI195_09085"
     CDS             complement(2373..>2725)
                     /locus_tag="VI195_09085"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY6824583.1"
                     /translation="SPFLQMDGQAVFKFAVRVLEESARETVAKAGMAMADVDWLIPHQ
                     ANVRILEAVARKLDLPREKLVVTVDHHGNTSAASVPLALDEYLRAGRIRPGQRLLLQG
                     VGGGFTWGSSLLTL"
BASE COUNT          410 a          986 c          861 g          468 t
ORIGIN      
        1 ccttggcgcc gacctcgatc gacagcagcc cggcgatcat gttgatgatg ctgccgggaa
       61 tgaagaaggg cgagatgcgc cgcgggccgt tcttgccgag ctcgtcgtgc atctgctcga
      121 tgagcggcag gccgccgatg cccgagccga aattgacgcc gacccgctcg gcgttctccg
      181 gcgtcacctg caagccgctg tcgcgccacg cctgcaggcc ggccgccatg ccgtagtgga
      241 tgaagagatt catgcgccgc gcttccttcg gcgacatgta aggcgcgacg tcgaagcctt
      301 tcacctcgcc ggcgatctgg ctcgcgaggc gcgaagcgtc gaagcgtgtg atgcgcgtga
      361 tgcccgaccg tccggcgagg cagttctgcc acgcctcgga aacgctgttg ccgatcgggc
      421 tgacgatgcc cagcccggtg acgacgactc tgcggcgtgt cacttaggac ttggcgtgcg
      481 ccttgatgta atcgatggcc tgctgcaccg tggtgatctt ctccgcctcc tcatcgggga
      541 tctcggtctc gaactcctcc tcgagcgcca tgacgagctc cacggtatcg agcgagtcgg
      601 cgccgaggtc atcgacgaaa gacgactcgt tcttgatctc ggcttcgttg acgcccagct
      661 gctcggcgat gattttcttg accgcttgct cgatgttctc catcgctgct ccttcccaaa
      721 aaggggccgt attttacgac atgtacatgc cgccgttgac gtgcaaaacc gcgccggtca
      781 cgtagcctgc gcccggcgaa acgagataac cgaccgccgc cgcgacgtcc gcggcgctgc
      841 ccagccggcc cagcgggatc tgggcgagca gcgcatttct ctgctcgtcc gacagcgcgc
      901 gcgtcatgtc ggtatcgatg aatccggggg cgatgcagtt gaccgtgatg ttgcggctgc
      961 cgagctcgcg cgcgagcgac ttggtcatgc ccaccacgcc ggccttcgcc gcggcgtagt
     1021 tcgcctgccc ggggttgccg gccgcgccga ccaccgacgt gatgttgacg atacggcccc
     1081 agcgcgcctt catcatgccg cgcatcaccg cgcgcgacag gaggaacacc gcgcgcaggt
     1141 tcgtctccat cacctcgtcc caatcgccat ccttcatgcg cagcgccagg ttgtcgcgcg
     1201 tgatgcccgc gttgttgacg agaatcgcga tgtcgcccac ctgcttctgg atgtcctcga
     1261 cgagcgcatc gcactgggcc gcgttcctga cgtcgagaac tctgccgctg cccggcacct
     1321 tggcggcgcc cgcctcgctc gtcgacgtac cgaccacctt ggcgccgagc cgcgcgagct
     1381 cttgcgcaat cgcctggccg atgccgcgcg aagcgcccgt caccagcgcc accttgcctt
     1441 ccagcatggt catcctttca tcgcgccgag cgcctgctcg acggcggcgc ggtccgagag
     1501 cgtgaacgac tggacctgcg gcgcgatgcg cttcgtcatg ccggcgagca ccttgcccgg
     1561 accgcattcg acgatgtgcg tagcgcccat ggccgccatc gcctggatcg tctcgaccca
     1621 gcgcaccggg gaggctgcct ggcgcaccag cgcgtcccgg atgcgcgacg gctcgctttc
     1681 catctggaca tcgacattgt tcaccacggc gcactccggc gcagcgaggg ttaccttggc
     1741 caggtaaacc tgcagacgct gcgccgcggg gcgcatcagc gtggagtgga aaggcgcgct
     1801 caccggcagc gccatcgcgc gcttggcgcc gtgcgccttg catttctcga ttgcccgctc
     1861 caccgccgcc ttctcgccgg caatgaccac ctggccgggc gcattgaaat tcaccgcctg
     1921 cacctcgccg tcggcttccg cgcacgccgc gcgcgccgcc tggtcgtcca tgccgaggat
     1981 ggccgccatg gcgccctgcc ccgccggtac cgcttcctgc atcgcctgcg cgcgaaagcg
     2041 aacgaggggc aggcagtcct tgaagccaat cgcgcccgcc gcggtaagcg ccgtgtactc
     2101 gcctagcgaa tgaccggcca tcacctcggg cgcgggcccg ccgagcgcgc gccaggcgcg
     2161 gtagaccgcg tagccggccg tcaccatcgc cggctgcgtg ttcaccgtga ggttgaggtc
     2221 gtcggccggc ccttcgtcca gcaggcgcac gaactccgcg cccagcaccg cccgtgcatc
     2281 ggcgcgcact tcgtcgattt ccggcaggcc gtcgtagccg cgcagcatgc cgaccgactg
     2341 cgagccctgg cccggaaaaa ccatggcgag cttcatagcg tcagcagaga ggagccccag
     2401 gtgaagccgc cgccgacccc ttgcaagagc agccgctgcc cggggcggat gcgcccggcg
     2461 cgcaggtact cgtcgagcgc tagcggcacg gaggcggccg aggtattgcc gtggtggtcg
     2521 accgttacca ccagcttttc gcgcggcagg tcgagcttgc gcgccaccgc ttcgagaatt
     2581 cgcacgttcg cctggtgcgg aatcagccag tcgacatccg ccatcgccat gcctgctttg
     2641 gcgacggtct cgcgcgcgga ctcctccagt acccgcaccg cgaacttgaa caccgcctgc
     2701 ccatccatct gcaggaacgg cgatc
//
LOCUS       DATDMP010000520         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1527029, whole genome shotgun sequence.
ACCESSION   DATDMP010000520 DATDMP010000000
VERSION     DATDMP010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527029"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1777
                     /locus_tag="VI195_09090"
     CDS             239..1777
                     /locus_tag="VI195_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6824584.1"
                     /translation="MADTPQAAGPDLQLGIPEQDLLDGSMLVGHVAQHAVLIARRGAE
                     LFAIDATCTHYGGPLGEGLLVAETVRCPWHHARFSLRSGEALAAPALAPVHCWRIERE
                     NGVVRVRERVDVKAQRPAPPPRAVRESAERIVILGGGAAGFAAAEMLRREGFGGELTV
                     ISADESAPYDRPNLSKDYLAGTAPEEWIPLRPPEFYAHNNIDLRLGTEATAIETEARR
                     VRLAGGEALPYTKLLLATGAEPVRLEVPGGNLPHVRTLRSLADCRAIIERTKPASRVV
                     IVGASFIGLEAAASLRQRRAEVHVVAPEPRPMERVLGAALGEFIRSLHESHGVVFHLE
                     RTVAAVEAQHVQLSDGTRLAADLVVVGIGVRPRTALAEKAGLAIDRGVLVNEYLETSA
                     AGVFAAGDIARWPDPHSGERLRIEHWVVAERQGQSAARNMLGRHEPFAKVPFFWTQQY
                     DVSVDYVGHASAWDSIEQDGDPRARDVACRFRRDGRTLAVATIFRGRESLQAEVAMEE
                     GGSP"
     gene            complement(1795..2382)
                     /locus_tag="VI195_09095"
     CDS             complement(1795..2382)
                     /locus_tag="VI195_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY6824585.1"
                     /translation="MSRLHRRLLATLGVLIVASLCIAAEMPTDSVYRLPAQLTTQRAA
                     SAGLDLYRGHPTLISMFYGSCPAACPMLITAMQVYEAHLDASSRAQLRVLLVSFDAAR
                     DTPRQLDRLAHLHHVDPERWTFASASEPSARRIAAVLGISYRHLASGEFDHSLLITLL
                     DDEGRIVASTTTLVGDEEFQARLRAATQPRAGEAQ"
     gene            complement(2379..>2569)
                     /locus_tag="VI195_09100"
     CDS             complement(2379..>2569)
                     /locus_tag="VI195_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824586.1"
                     /translation="NFNSLLPADADPEKFCGSSALDLQQKENYAVLMRIAMLGSLRAS
                     HTGRTVGFRCTRDAGNSP"
BASE COUNT          419 a          841 c          905 g          404 t
ORIGIN      
        1 cgagtgcccg atgggcgcat cggacgcgta cgcgggcgca gcggtcgact catcaaggtc
       61 cgcgtccggc ggcagcgcag cgacactcat cagttcctga tgtgcgatcc aatgacctta
      121 gcgcgcgtgg actgcccgaa gggctggatg agcccgcagg gctatcaacg atatctgcgc
      181 gtcacgctcc gcaagatgcg tgcgcgggcc gcagcaaaac cgcatagagg acatcgccat
      241 ggctgatacg ccgcaagccg caggacccga tttgcaactc ggcattccgg aacaggatct
      301 gctcgatggc agcatgctgg tgggacacgt ggcgcagcat gcggtgctca tcgcgcgtcg
      361 cggcgcggag ctctttgcca tcgacgccac ctgcacgcac tacggcgggc cgctcggcga
      421 aggcctgctg gtcgcagaga cggtccgctg cccctggcac catgcccgct tcagcctccg
      481 ctcgggtgaa gcgctcgcag cgcccgcact cgcgccggtg cactgctggc gcatcgagcg
      541 ggagaacggc gtggtgcggg tgcgcgagag agtcgatgtg aaggcacagc ggccggcgcc
      601 gccgccgcgt gccgtacggg aaagcgctga gcgcatcgtc attctcggcg gtggggcggc
      661 gggttttgcc gccgccgaga tgctgcgccg cgagggcttc ggcggtgagc tgaccgtgat
      721 cagtgcggac gagtcggcgc cgtacgaccg gcccaatctc tcgaaggact atctggcggg
      781 caccgccccg gaagagtgga ttccgctgcg gccgcccgag ttctatgcac acaacaatat
      841 cgatctgcgc ctcggcacgg aggcgacggc catcgagacc gaagcccggc gtgtgcggct
      901 ggcggggggc gaggcgctcc cctataccaa gctgctgctc gctaccggcg ccgagccggt
      961 gcgcctcgag gtgccgggcg ggaatctccc acacgtgcgc acactgcgct cactggctga
     1021 ttgtcgggcg attatcgagc gcaccaagcc tgctagccgc gtcgtgatcg tcggcgcgag
     1081 cttcatcggc ctcgaggcag ccgcgtcact gcgccagcgg cgcgccgagg tgcacgtggt
     1141 cgcgcccgag ccacggccca tggagcgcgt gctcggagcg gcgctgggcg agttcatccg
     1201 ctcgctgcac gaatcgcacg gtgtcgtgtt ccacctggag cgtacggtcg ccgccgtcga
     1261 ggcgcagcac gttcagctca gcgatggcac gcgcctcgct gccgatctcg tggtggtggg
     1321 catcggcgtg cggccgcgta ccgctctcgc cgagaaggcg ggactggcca tcgatcgtgg
     1381 cgtgctggtc aatgagtatc tggagaccag cgctgcagga gtcttcgctg ccggcgacat
     1441 cgcccgctgg cccgaccctc acagcggcga gcggctgcgc atcgagcact gggtcgtcgc
     1501 cgagcgccaa gggcagagcg ccgctcggaa catgctcgga cgccacgagc ctttcgccaa
     1561 ggtaccgttc ttctggaccc agcagtacga tgtgtcggtc gactacgtcg gccacgcatc
     1621 agcctgggac agcatcgagc aggacggaga cccgcgagcc cgcgatgtgg catgccgctt
     1681 tcggcgcgac ggccgcacgc tggcggtggc gaccatcttc cgtggccgcg agagcctgca
     1741 agcggaagtc gccatggaag agggtggatc gccgtaggcg gcggagccct cagctcactg
     1801 cgcctcgcct gcgcgcggtt gcgtggcggc gcgcagcctc gcctgaaatt cctcatcgcc
     1861 cacgagcgtc gtggtgctgg cgacgattcg gccctcgtca tcgagcagtg tgatgagcaa
     1921 cgagtggtcg aactcgccgc tcgccagatg gcggtagctg ataccgagca cggctgcgat
     1981 gcggcgcgcg ctcggctcgg aggcgctggc gaatgtccag cgctcggggt cgacgtggtg
     2041 caggtgcgcc aagcggtcga gttgtcgcgg ggtatcgcgt gcggcatcga agctcaccag
     2101 cagcacccgc agctgcgccc gcgacgatgc gtcaagatgc gcttcataca cctgcatcgc
     2161 ggtgatcagc atggggcaag ctgccggaca gctgccgtag aacatgctga tcagcgtggg
     2221 atgaccgcgg tacaggtcga gtccagcgct tgccgcgcgc tgcgtagtga gctgcgccgg
     2281 cagccgatac accgagtcgg tcggcatctc ggcagcgata caaagcgatg ccacgatcag
     2341 cacgccgagg gtggcgagca gcctgcggtg caaccggctc acggcgaatt cccggcatcg
     2401 cgggtacagc gaaaaccgac ggtgcgtccc gtgtgcgagg cgcgcagact gccgagcatc
     2461 gcaatgcgca tcagcaccgc gtagttctct ttctgctgca ggtcgagcgc actgctgccg
     2521 cagaacttct ccgggtcggc atcggcaggt aacagggaat tgaagttat
//
LOCUS       DATDMP010000521         6567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_639822, whole genome shotgun sequence.
ACCESSION   DATDMP010000521 DATDMP010000000
VERSION     DATDMP010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639822"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..220
                     /locus_tag="VI195_09105"
     CDS             20..220
                     /locus_tag="VI195_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824587.1"
                     /translation="MLAEAMEHFALSGRARQRVLRVARTIADLAAAPSITSGHIGEAL
                     LLRGLDRQLTTAPSAACDRRLA"
     gene            complement(180..698)
                     /locus_tag="VI195_09110"
     CDS             complement(180..698)
                     /locus_tag="VI195_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6824588.1"
                     /translation="MSKQDTHFFNVFSLVIALLVAVAIGLFALARIVASHTQERQVLS
                     EADYAKNVALRLTPPAQEAVAGQDNSALAIKAPATAAAGSAAAVPKNGTELFDQTCSA
                     CHGQGIAGAPRAGDKAAWGPRIAKGKSVLYDHAIHGFTGTAGMMPAKGGRTDVSDDLV
                     KQAVDHMLQMAQ"
     gene            855..931
                     /locus_tag="VI195_09115"
     tRNA            855..931
                     /locus_tag="VI195_09115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:889..891,aa:Arg,seq:ccg)
     gene            complement(1075..1959)
                     /locus_tag="VI195_09120"
     CDS             complement(1075..1959)
                     /locus_tag="VI195_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY6824589.1"
                     /translation="MTIRLLAIAALLALSACARHETPPAPAAAQPAPAGTSATATPPA
                     ATPPSAEARSPQSETEQATASQESGDGDTERQARSDASLEKIAGATSAAPLPAGKWQA
                     GVNYDPVVPAQPTSVSPGKVEVTEVFWLACPHCYALEPRVRSWLQSKPAYVEFVRVPV
                     IWQPVHRAHARLFYTLEALNRDDLVGKAFDTIHQDLQNQVPPLVGQSEEETYRLQQQF
                     ATQNGISADDFSKAYNSFSVSSNLQRAEEITQRYHVQGVPFFVVNGKYSSDVAKAGSE
                     AKLIELINDLAASEHASH"
     gene            complement(1974..2636)
                     /locus_tag="VI195_09125"
     CDS             complement(1974..2636)
                     /locus_tag="VI195_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6824590.1"
                     /translation="MSLAALPGIGQTPAPASASAAAPSAPADAPFKDGDAARGTAKAA
                     VCSACHGPNGNSSSPEWPRLAGQSAVYIVEQLRLFRSGVRNNPVMKPLASSLSDQDID
                     DLALYYEAQTPAGLEADPSYWQAGEALYLRGDRAHAVPACVACHGPVGRGNLAAGYPA
                     LRAQQSVYVVKQLNDYASGARYTGANPAAASRNGLMMFTIARRLSPEQIRDVASYVQG
                     MR"
     gene            2777..3379
                     /gene="yihA"
                     /locus_tag="VI195_09130"
     CDS             2777..3379
                     /gene="yihA"
                     /locus_tag="VI195_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY6824591.1"
                     /translation="MSRFGEARFLLSVASPAQLPPDEGAEVAFAGRSNSGKSSAINAI
                     TRRRGLARTSKAPGRTRLLNFFELAPARRLVDLPGYGYASVTAAERAAWLPLLEALRV
                     RSSLKGLLLVVDSRRGLTQMDEGLMEWVPGGQGLHVLLSKADKLNRGEASAALRSASA
                     QLGNRGTVQLFSALHGTGVREAQERIAAWLSVAPAGSSGQ"
     gene            complement(3612..4172)
                     /locus_tag="VI195_09135"
     CDS             complement(3612..4172)
                     /locus_tag="VI195_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6824592.1"
                     /translation="MRMRTLMPLTVLAFAAGAAQAENGFFYLGAGVVRNSLSDITSIG
                     GLPDLQNTSWKAYAGVRPLNWLAAEADYIDLGSNSGTNASGSSATTNGSAFAAYAVGF
                     LPIPLPIVDIFGKVGLARWKYDGNVIPPFGSSGTSFSTNGTDFAWGIGVQAHISLIGA
                     RLEYENFNVPNTSGARVASLSVFLNL"
     gene            complement(4288..>6567)
                     /gene="polA"
                     /locus_tag="VI195_09140"
     CDS             complement(4288..>6567)
                     /gene="polA"
                     /locus_tag="VI195_09140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY6824593.1"
                     /translation="VLDREGVKLKFDVYPEQIIDYLALVGDSSDNIPGIDKVGPKTGA
                     KLLQQYGGLDQLIAHLAEVGGKVGENLRAGLDTLELSRRLATIRTDLELAVSLEELTP
                     RAAQVAKLRELYQQLELRGLLRQLEAGEAGSGTDAPAAPPPLAAAAASVPEAPPIERH
                     YETISDWDAFERWLSLLQDAELFAFDLETTSPDYMRAEIVGLSFSLDAGRAAYVPLAH
                     AYPGAAEQLPRGQVLARLKPLLEDPARGKVGHDLKYVAHTLAHAGITLRGMRFDTMLE
                     SYVWNSVATNHEMDSDAQRYLGVRTLNYTDLTGKGAKQIGFDQVPVERASEYACEHTD
                     VTLRLHHELWSQLSGIPALARLYAEIEQPLVPVLLAMEQHGVLVDREQLRLQSREFSR
                     QLQELLLQAHREAGHEFNIDSPRQLQQILFERLQLPVRRRTPTGQPSTAEDVLEDLAA
                     SYALPRLVLEYRALAKLKSTYTDKLPELVNERTGRIHTSYAQAVAATGRLSSVEPNLQ
                     NIPIRHPEGRRIRKAFIAQPGAVLLAADYSQIELRIMAHLSGDDSLRAAFAEDRDVHQ
                     ATAAEVFGVELAAVSADQRRTAKVINFGLIYGMSAFGLARNLGIERSAAAQYVERYFQ
                     RYPGVRRFMDDTRVQARELGFVETVFGRRLYLPDIRSGNPQLRQYAERAAINAPMQGT
                     AADIVKRAMIRVHEWCMREAAPARLMMQVHDELVLEVRADAVDRVAAAVREHMLQAAT
                     LSVPLRVDVGTGANWDEAH"
BASE COUNT         1051 a         2126 c         2250 g         1140 t
ORIGIN      
        1 ggacgagctg ggtcagcgcg tgctagcgga ggcgatggag cactttgctc tttccgggcg
       61 tgcccggcag cgggtgctga gagtggcgcg cacgattgcc gatctcgcag ccgcaccgtc
      121 catcacttcg ggtcacatcg gcgaagcgtt gctgctacgc ggactcgacc ggcagctgac
      181 tactgcgcca tctgcagcat gtgatcgacg gcttgcttga cgaggtcgtc ggacacatcc
      241 gtgcgcccgc ctttcgccgg catcatgccg gccgtccccg tgaagccatg gatcgcgtgg
      301 tcgtagagca ccgacttgcc cttggcaatt ctcggccccc atgcagcctt gtcgccggcg
      361 cgcggtgcgc cggcgatgcc ctgcccgtgg caggcgctgc aggtctgatc gaaaagctcg
      421 gtgccgttct tcggaaccgc tgcggcgctg ccggcagcag ctgtggccgg ggccttgata
      481 gccagggccg agttgtcctg gccagcgacg gcttcctggg ccgggggagt gaggcgcagg
      541 gcgacgttct tcgcgtagtc ggcctcactc agcacctgcc gttcctgcgt gtggctggcc
      601 acgatgcgcg cgagcgcgaa caggccgatc gccaccgcca ccagcagtgc aatcacgaga
      661 ctgaaaacat tgaagaagtg ggtgtcctgc ttgctcacga tccaacccgc tggccggccc
      721 ccggaaatgg ccgcgcagta tagccgttcg cacctgtgat cgcgtaaggc gcttggcgcc
      781 tgcggcgcca caacggccgg cggagcggcg ctggcagcgc gggggcggcc tcgcttatga
      841 tcgccggccc caacgcgccc gtagctcagt tggatagagt ggcagcctcc gaagctgcag
      901 gtcgctggtt cgaatccagt cgggcgcgcc aaagaaatca agcggttgtg tgcccgccag
      961 cgcaggttag atcgtgcggt tcgcactcgg cggcgttagg aactcctcca gatcctggtc
     1021 agtgcgtcaa gcacccccag cacgatcgca gcggcgagca gcgcagcggc ctgctcagtg
     1081 gctggcgtgc tcggaagccg cgaggtcgtt gatcagctcg atgagcttcg cttcactgcc
     1141 ggccttggcg acgtcgctac tgtacttgcc gttcaccacg aagaatggca cgccctgtac
     1201 gtggtagcgc tgggtgatct cctcggcacg ctgcagattg gagctgaccg aaaaggagtt
     1261 gtaggccttg gagaaatcat cggcactgat gccgttctgc gtggcgaact gctgctgcag
     1321 ccggtaagtc tcttcctcgc tctggccgac gagcggcggc acctgattct ggaggtcctg
     1381 gtgaatcgtg tcgaaggcct tgccgaccag atcgtcgcga ttgagggcct cgagggtgta
     1441 gaacagccgc gcgtgggcgc ggtgaaccgg ctgccagatc accggcactc tcacgaactc
     1501 gacgtacgcg ggcttgctct gcagccagct gcgcacgcgc ggctcgaggg cgtagcaatg
     1561 cgggcacgcg agccagaaca cttccgtcac ttccactttg ccgggtgaca cactggtcgg
     1621 ctgcgccggc accacggggt cgtagttcac acccgcctgc cacttgcccg cgggcagtgg
     1681 agcggcagat gtagcgccgg cgatcttctc cagcgaagca tcgctgcgcg cctgccgctc
     1741 ggtgtcgcca tcgccggact cctgcgacgc agtcgcctgc tcggtctcac tttgcggcga
     1801 acgcgcctct gcagaggggg gcgtggcagc gggcggcgta gccgtggccg acgtgccggc
     1861 cggcgcaggc tgcgcggccg cgggtgcagg cggcgtctcg tggcgtgcgc aggcagacag
     1921 agccagcagc gccgcaatgg cgagtagacg aatcgtcatg cgaggtcctc gagtcagcgc
     1981 ataccctgga cgtaggaggc tacgtcgcgg atctgctcgg gcgagagccg tcgtgcaatg
     2041 gtgaacatca tcaagccgtt gcgcgatgcc gccgccggat tggcgcccgt gtagcgcgca
     2101 ccgctcgcgt agtcattgag ctgcttcacc acgtacaccg actgctgcgc tcgcaaagcc
     2161 gggtaacccg cggcgagatt gccacgcccc accgggccgt gacaggcgac gcacgccggc
     2221 acggcgtgcg cgcggtcacc acgcagatac agcgcctcgc cggcctgcca ataggatggg
     2281 tcggcctcga gtccggctgg agtctgcgct tcgtagtaca gcgccagatc gtcgatgtcc
     2341 tggtcgctga gggagctcgc gaggggtttc atgaccggat tgttgcgcac accggagcga
     2401 aacaggcgca gttgctcgac gatgtagacc gcgctttgcc ccgcgagccg cggccactcg
     2461 gggctcgagc tgttgccgtt cggcccgtga caggccgagc acaccgccgc cttcgcagtg
     2521 ccgcgcgcgg catcgccatc cttgaatggg gcatccgcag gggcggacgg agcggcggcg
     2581 ctcgcagaag ccggtgccgg tgtctggccg atgccgggca gcgccgccaa cgacacagag
     2641 gccgccgtgg caagccacct cacccaacag actgttcggc tctgccgcat tgtgtgcttt
     2701 ccgctgacgg acccgtgcgc aagggcgccg aattgtacac tacggttgtg cccgcgcttc
     2761 gttactcctt gaaaacatga gccgctttgg cgaggcgcgg ttcctcctga gtgtggcatc
     2821 accggcgcag ttgccgccgg atgagggagc ggaggtggca ttcgctggac gttccaactc
     2881 gggtaagtcg agtgccatca acgcgatcac acggcgtcgg ggattggccc gcacgagcaa
     2941 ggcgccgggt cgcactcggc tgctgaactt cttcgagctc gcacctgcca ggcgcctggt
     3001 ggatcttccg ggctacggct atgcgagcgt cacggcggcc gagcgtgcag cctggttgcc
     3061 gctcctcgaa gcgctgcggg tacgcagctc tctcaagggc ttgctcctcg ttgtcgactc
     3121 acggcggggt ttgacgcaga tggacgaggg gctcatggaa tgggtgcccg gtggtcaagg
     3181 ccttcacgtt ctgctttcca aggcggacaa gctcaaccgg ggcgaagcct ccgccgcact
     3241 gcgctcagcc tcggcgcagc tcggcaatcg tggcacggtt cagttgttct ccgcgctcca
     3301 cggcacgggg gtccgggaag cacaggagcg gatcgcggcg tggctgtcgg tcgcgcctgc
     3361 tggatcgagc gggcaataaa aaaccccggc ggtgttaacc gccggggcag actaacccgg
     3421 cacaggtctc atgaaccggg tctacccgct cagggaggga agcggggagt gctatgcact
     3481 cgttaagttc gacgccggtg gctgaccgga gttccacgcc cgctttgaaa aaatatcttt
     3541 ttaaatcata agtttgctta agcaattgtc tctttggcgc cacagactcg agctttacct
     3601 ctgccgtatt gtcagaggtt tagaaacacc gagagcgagg ccacccgtgc gccactggta
     3661 ttcggaacgt tgaaattctc gtattccagg cgggcgccga tcaggctgat gtgcgcctgc
     3721 acgccgatgc cccaggcgaa atccgtgccg ttggtcgaga acgaggtccc cgaggagccg
     3781 aacggtggga tgacgttgcc gtcgtatttc cagcgcgcga ggcccacctt gccgaaaatg
     3841 tccacgatcg gcagtggaat cggcaggaag ccaacggcat acgcggcgaa cgcgctgcca
     3901 ttcgtggttg cgctcgagcc ggaggcgttg gtgccgctgt tgctgccaag atcgatgtag
     3961 tccgcttcgg cggccagcca gttgagcgga cgcacgccgg cgtacgcctt ccacgaagtg
     4021 ttctgcaggt cgggcagccc gccgatcgag gtgatgtcgc tcagcgaatt gcggaccaca
     4081 cccgcgccca ggtagaaaaa tccgttctcg gcctgcgcgg caccggcggc gaacgccagc
     4141 acggtgagcg gcatgagtgt gcgcatacgc atgaacgaac ctcgcgtggt gaaggcagct
     4201 gctggaaaaa cgccggcgcg cagcgggcgc tgacacacct ggacgctgaa acggcaacgt
     4261 tgccgtgggc agtatccctt gccggggtca gtgcgcctca tcccagttcg caccggtgcc
     4321 cacatcgaca cgcagcggga cgctcagtgt tgccgcctgc aacatgtgct cgcgcaccgc
     4381 cgcggcgact cggtcgaccg catccgcgcg cacctccagc acgagctcgt cgtgcacctg
     4441 catcatgagc cgcgccggtg cggcctcgcg catgcaccat tcgtgtacgc ggatcatggc
     4501 gcgcttgacg atatccgctg ccgtgccctg catcggggcg ttgatggcgg cgcgctcggc
     4561 atactggcgc agctgcggat tgccggaacg gatgtcgggc aggtacaggc gccggccaaa
     4621 caccgtctcg acgaacccca gctcgcgtgc ctgcacgcgc gtgtcgtcca tgaaacgccg
     4681 cacgccggga tagcgctgga agtagcgctc gacgtactgc gctgccgcgc tgcgctcgat
     4741 accgaggttg cgtgcgaggc cgaaggcgga catgccgtag atgagaccga aattgatcac
     4801 cttcgcggtg cggcgctgat cggcgctgac ggccgcgagc tcgacaccga aaacctcggc
     4861 ggcggtcgcc tggtgcacgt cgcggtcttc ggcgaaggct gcgcgcaggc tgtcatcgcc
     4921 cgagaggtgc gccatgatgc gcagctcgat ctgtgagtag tcggccgcca gcagcaccgc
     4981 gccgggctgg gcgatgaagg ccttgcggat ccggcgtcct tcggggtgcc gtatcggaat
     5041 gttctgcagg ttcggctcca ccgacgagag tcggccggtc gcggcaaccg cctgtgcgta
     5101 actggtgtgg atgcgtccgg tacgctcgtt caccagttcc gggagcttgt cggtataggt
     5161 ggatttgagc ttggcgagcg cacggtactc gagcaccagg cgcggcagcg cgtagctggc
     5221 agcgagatcc tcgagcacat cctccgcggt cgagggctgg ccggtcggcg tgcgccgccg
     5281 caccggcagc tgcagccgct cgaacaggat ctgctgcagc tggcgcggcg agtcgatgtt
     5341 gaactcgtgc cccgcctcgc ggtgcgcctg cagcagcagc tcctgcagct ggcgcgagaa
     5401 ttcacggctc tgcaggcgca gctgctcacg atcgacgagc acgccgtgct gctccatggc
     5461 gagcagcacc ggcacgagcg gctgctcgat ctccgcatag agccgggcga gtgcgggaat
     5521 gccgctgagc tgcgaccaca gctcgtggtg caggcgcagc gtcacatcgg tgtgctcgca
     5581 cgcgtactcg ctcgcgcgct ccaccggcac ctgatcgaag ccgatctgtt tcgcgccctt
     5641 gccggtgagg tcggtgtagt tgagggtgcg cacgccgaga tagcgctgcg cgtcggagtc
     5701 catctcgtgg ttggtcgcga cgctgttcca cacgtacgac tcgagcatgg tgtcaaagcg
     5761 catgccgcgc agcgtgatcc cggcatgcgc gagcgtgtgc gcgacgtact tcagatcgtg
     5821 gcccaccttg ccgcgcgcgg gatcctcgag cagtggtttg aggcgcgcca gcacctgccc
     5881 gcgcggcagc tgctccgcag caccggggta ggcgtgtgcg agcggcacgt acgcggcgcg
     5941 gcccgcatcg agactgaagg agagtccgac gatctcggcc cgcatgtagt cggggctggt
     6001 ggtctcgagg tcgaacgcaa acagctcggc atcctgcagc aggctcagcc agcgctcgaa
     6061 cgcatcccaa tcacttatcg tctcgtaatg gcgctctatg ggcggcgcct ccggcacgct
     6121 cgccgcggcg gccgctagcg gcggcggcgc cgccggcgca tccgtcccgg atccggcttc
     6181 gcccgcttcc agctggcgca gcagtccgcg caattcgagt tgctgataga gctcgcgcag
     6241 cttggcgacc tgcgcagcgc gtggcgtgag ctcctcgagg gagacggcga gctcgagatc
     6301 ggtgcggatg gtggcgaggc gccgcgagag ctcgagcgtg tcgagtccgg cgcgcaggtt
     6361 ctcgcccacc ttgcccccga cctccgccag gtgcgcgatc agctgatcga gcccgccgta
     6421 ctgctgcagg agcttcgcgc cggtcttcgg tccgaccttg tcgatgccgg ggatgttgtc
     6481 ggagctgtcg ccgaccagcg ccaggtagtc gatgatctgc tcggggtaga cgtcgaactt
     6541 gagcttcacg ccctcgcggt cgagcac
//
LOCUS       DATDMP010000522         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_84775, whole genome shotgun sequence.
ACCESSION   DATDMP010000522 DATDMP010000000
VERSION     DATDMP010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84775"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VI195_09145"
     CDS             complement(<1..795)
                     /locus_tag="VI195_09145"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY6824594.1"
                     /translation="MSAFELLAASPAAFIGACLALGLVVGSFLNVVIYRLPLMLERQW
                     REQCAEESEATAVAQPASAAAPTANGGTVARAVAPLDERFNLVVPRSACPVCRAPIRA
                     FHNIPLLSWLLLRGKCASCAAPISTRYPLVEALTGVLSALIAWKFGFDWAAVAGLLVT
                     WFLIALAFIDIDHQLLPDSLTLPLLWLGLFLSLFSAQSSGPALPVDTRASLIGAMAGY
                     VSLWSVYHLFRLLTGKDGMGYGDFKLFAALGAWFGWQMLLPIILIAS"
     gene            complement(868..2100)
                     /locus_tag="VI195_09150"
     CDS             complement(868..2100)
                     /locus_tag="VI195_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6824595.1"
                     /translation="MAPAATATVKAIKREIPFAWEGRDKRGARIKGRSLAPDEQTLRA
                     ELRRQGVAPSKIRKQRQGRKGGKVNPGDIAVFSRQLATMLAAGIPLVQAFEIVGAGHE
                     KPAMQKLILDVKADIEGGTSLHEALAKHPLYFDDLYVNLVEAGEQAGALESLLDKIAT
                     YKEKTESLKKKVKKALFYPAAVLVVAVIVTIVLLVFVIPQFESLYKGFGANLPAFTQF
                     VIHISQIVQHDGLFMAMVVGGIGWAFFYFKKRSRALREFLDRLILKLPIIGPIMNKAA
                     IARYARTLSTMFAAGVPLVEALDSVAGATGNIVYENAVIKMRDEVSTGQRLQRAMENT
                     ALFPNMVNQMIAVGEESGSLDEMSGKVASFYEAEVDNAVDAMSSLLEPLIMLILGVLV
                     GGLVIAMYLPIFKLASVV"
BASE COUNT          436 a          727 c          724 g          393 t
ORIGIN      
        1 cgaggcgatc aggatgatcg gcagaagcat ctgccaaccg aaccacgcgc ctagtgctgc
       61 aaagagcttg aagtcgccgt agcccatgcc gtccttaccg gtgagaaggc gaaacagatg
      121 atagacgctc cacaggctca cgtaacccgc catcgcgcca atcaggctcg cgcgcgtgtc
      181 cacgggcaga gcgggcccgg agctctgcgc gctgaagaga ctgaggaaca agccgagcca
      241 cagcagggga agggtcaggc tgtcgggaag cagctggtga tcgatgtcga tgaaagcgag
      301 ggcgatcagg aaccaggtga ccagcaagcc cgcgaccgcc gcccagtcaa agccgaattt
      361 ccacgcgatc agtgccgaca gcacaccggt gagggcttcg acgagcgggt aacgcgtgct
      421 gatcggtgcc gcgcagctcg cgcacttgcc gcgcagcagc agccaggaga gcagcggaat
      481 gttgtgaaat gcgcggatcg gcgcgcggca cacggggcag gcggagcgcg gaaccaccag
      541 attgaagcgt tcgtcgagcg gcgcgaccgc tctggccacg gtgccgccgt tcgctgtcgg
      601 tgccgccgcg ctcgccggct gcgccacagc ggttgcctcc gactcctccg cgcactgctc
      661 gcgccattgc cgctcgagca tgagcggcag acggtagatc accacgttca gaaagctgcc
      721 gaccacaagt ccgagtgcca ggcaagcacc gatgaaggcg gccggggaag ctgctagaag
      781 ctcgaaggcg gacacgtatg gcttatccta atgaaaacgc cgcgcaccgc gcggcgtttt
      841 ccgcaggcgg ctggccgggt gcaggcgcta caccaccgat gccagcttga agatcggtag
      901 atacatggcg atcacgagac caccgaccag cacaccgagt atgagcatga tgagcggctc
      961 gagcaggctc gacatggcat cgacggcgtt gtccacctcg gcctcgtaga agctggccac
     1021 tttgcccgac atctcatcga gcgaaccgga ttcctcgccc accgcgatca tctggttcac
     1081 catgttggga aacagggcag tgttctccat cgcgcgctgc agccgttggc cggtggacac
     1141 ctcatcgcgc attttgatga ccgcgttctc gtagacgatg ttgcccgtgg cgccggcaac
     1201 ggagtcgagc gcttcgacca gcggcacccc ggcggcgaac atggtggaga gcgtgcgcgc
     1261 gtagcgtgca atcgccgcct tgttcatgat cgggccgatg atcgggagct tcagtatcaa
     1321 gcgatcgagg aactcccgca gcgcccgcga gcgcttcttg aagtagaaaa acgcccagcc
     1381 gataccgccg acgaccatcg ccatgaacag gccatcgtgc tggacgatct gagagatatg
     1441 gatcacgaac tgagtgaagg ccggcagatt cgccccgaag cccttgtaga gcgactcgaa
     1501 ctgcggaatg acgaagacga gcagcacgat cgtcacgatc accgccacca ccagcaccgc
     1561 ggccgggtag aacagcgcct tcttgacctt cttcttcagc gattcggttt tttccttgta
     1621 ggtggcgatc ttgtcgagca gggactcgag cgcacccgcc tgctcaccgg cttcgaccag
     1681 gttcacgtac agatcgtcga agtagagggg gtgcttagcg agcgcttcgt ggagtgaggt
     1741 gccgccctcg atgtccgcct tgacgtcgag gatgagcttc tgcatcgccg gcttctcgtg
     1801 gcctgcgccg acgatctcga acgcctgcac gagtggaata cccgccgcca gcatggtggc
     1861 gagctgacgg ctgaagaccg cgatgtcgcc tggattgacc ttgccgccct tgcgcccctg
     1921 gcgctgcttg cggatcttcg agggcgcgac accctgacgg cgcagctccg cgcgcagcgt
     1981 ctgctcatcg ggtgccaggc tccggccctt gatgcgcgcg ccgcgcttgt cccggccctc
     2041 ccaggcgaag gggatttcgc gcttgattgc tttgacggtt gccgttgctg ccggggccat
     2101 atgagtttca ccctgccgtt attcaaccgt gacgctgttg atttcctcga gactagtgag
     2161 gccactcttc actttctcga gtcctgcacg ccgcaggtcc cagacgcctt ccttggcggc
     2221 ctgctgcccg atctccacgg cgctgccgcc ggcgagaatg atgcgggcaa tcgaatcggt
//
LOCUS       DATDMP010000523         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1307208, whole genome shotgun sequence.
ACCESSION   DATDMP010000523 DATDMP010000000
VERSION     DATDMP010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307208"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..821)
                     /locus_tag="VI195_09155"
     CDS             complement(87..821)
                     /locus_tag="VI195_09155"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6824596.1"
                     /translation="MCSEAGDRPVQSGGATGERAAGPGAADEDARLARRVLAGDRSAF
                     ELLMRRYNRRLYRLARASLGDDAEAQDALQDAYLCAYRSLGQFRGDAALSTWLSRLVL
                     NACGARRRRSLRRASIVPIVGSNYAMDALAAVPDSGDQPDRLAARSQIRSVLERKVSE
                     LPEDFRIVFVLRSVEELSVEEIADTLELSPETVRSRHFRARGMLRASLAKDIDLAEGD
                     VFEFGGGCCDRMVARVLAILEQHGTG"
     gene            complement(811..1149)
                     /locus_tag="VI195_09160"
     CDS             complement(811..1149)
                     /locus_tag="VI195_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEY6824597.1"
                     /translation="MRAAARCALVVRAALLTSGALIPAAGNAAQPQHTVTIDGVQYSP
                     PELTVRRGERIVWVNKDPFPHTVTAAAGQFDSGSIAAGASWTFRAAKRGEYAYGCRFH
                     PTMKGTIHVQ"
     gene            complement(1146..1679)
                     /locus_tag="VI195_09165"
     CDS             complement(1146..1679)
                     /locus_tag="VI195_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY6824598.1"
                     /translation="MKIIASCTAILLGVSVSGAWAQASGPTDPQIAAIVVTANQVDID
                     AGKLAKSRTHSKTVREFATRMIVDHTGVNKSATELVHKLHVTPEPNPTSEELQKGGDE
                     NLAKLRGLSGKAFDQAYVDHEVAYHEAVIQALDKTLIPSAHNSELKALLIKVRPAFIA
                     HLDHARRLQAELGKSSG"
     gene            complement(1779..>2030)
                     /locus_tag="VI195_09170"
     CDS             complement(1779..>2030)
                     /locus_tag="VI195_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824599.1"
                     /translation="QLGLHFIPLVEEDYFLACFEADAVRPAVMRLRASLAGAGWRQIL
                     ASLPGYRPPEAPGDLLAVEQALPWWRNCRPLPPAGTGAP"
BASE COUNT          296 a          725 c          686 g          323 t
ORIGIN      
        1 tggcggccgg cggggttgcc gggcgcatct gcacgggcgc ggccggggcg tgcatggacc
       61 gccgggccgc gcgcgcctga ccgcacctac ccggtgccgt gctgctccag gatcgcgagc
      121 acgcgcgcca ccatgcgatc gcagcagccc ccgccgaact cgaagacatc gccctccgca
      181 aggtcgatgt ctttcgcgag cgatgcccgc agcatccccc tggcacggaa atgccggctg
      241 cggaccgtct ccggcgagag ctcgagcgtg tcggcgatct cctcgacgct cagctcttcc
      301 accgagcgca gcacgaacac gatgcggaag tcctccggca attcgctcac ctttcgctcg
      361 agcacgctgc ggatctgtga gcgggccgcc agccgatccg gctggtcccc cgagtccgga
      421 actgccgcca gggcgtccat cgcgtagttc gagccgacga tgggaacgat gctcgcgcgt
      481 cggagcgagc ggcggcggcg cgcgccgcag gcgttcaaca ccagccgcga gagccaggta
      541 gagagcgccg cgtcgccacg aaactgcccc agcgagcgat aggcgcacag atacgcatcc
      601 tgcagcgcgt cctgtgcctc ggcgtcgtcg ccgagcgatg cccgtgccag gcgatacagg
      661 cggcggttgt agcgccgcat gagcagctcg aaggccgacc ggtctccggc cagcacgcgc
      721 cgcgcgagcc gcgcatcctc gtccgccgcc ccgggccccg cggcgcgctc accagttgcg
      781 ccgccggact gcaccggccg gtcgcccgcc tcactgcaca tggatcgtgc ccttcatggt
      841 cggatgaaag cggcagccgt aagcatactc gccgcgcttc gcggcgcgga aagtccagga
      901 tgcgccggcg gcgatgctgc cggagtcaaa ctgcccggcg gcggcggtga ccgtgtgggg
      961 gaacggatcc ttgttcaccc acacgatccg ctcgccccgg cgtacggtca gttccggcgg
     1021 actgtattgc acgccatcga tcgtgacggt gtgctggggc tgcgcggcgt tcccggcggc
     1081 gggaatcagt gcgccggatg tcagcaaggc cgcgcgcacc accagtgcgc agcgcgcggc
     1141 cgctctcatc cgctgctctt gccgagctcc gcctgcaggc gcctggcgtg atcgaggtgg
     1201 gcgatgaacg ccgggcgcac cttgatcagc agcgccttca gctccgagtt gtgtgcgctc
     1261 ggaatcaatg tcttatccag cgcctggatg accgcttcgt gataggcgac ctcgtggtcg
     1321 acataggcct gatcgaacgc cttgccggag aggccccgga gcttggcgag gttctcatcc
     1381 ccacccttct gcagctcttc gctcgtcggg ttcggttccg gcgtcacgtg aagcttgtgc
     1441 accagctcgg tggcgctctt attgactccc gtgtgatcga cgatcatccg ggtggcgaat
     1501 tcccgcactg tcttggaatg cgtcctggac ttcgcgagct tgccggcgtc gatgtcgacc
     1561 tggttcgcgg tgactacgat cgccgcgatc tgcgggtcgg tcggtccgga agcctgggcc
     1621 caggcgccgc tcacgctgac gccgaggagg atcgctgtac agctcgcaat gatcttcatt
     1681 tgtccactct ccgttaaacg ggtgctcccc gattagatgt cacgaccccg cgccccgttc
     1741 ccgcgctccg cttcccgcgc cgggcgggcg gcgcccggct agggtgcgcc ggtcccggcc
     1801 ggcgggagcg gccggcagtt gcgccaccag ggcagcgcct gctcgactgc cagcagatcc
     1861 cccggcgctt cgggcggccg atagccgggc aggctcgcca gaatctgccg ccagccggcg
     1921 ccggcgaggc tcgcgcgcag tcgcatcacc gccggccgga cagcatccgc ctcgaagcag
     1981 gcgagaaagt aatcctcctc gaccagggga atgaagtgca gtccgagctg
//
LOCUS       DATDMP010000524         2720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2196576, whole genome shotgun sequence.
ACCESSION   DATDMP010000524 DATDMP010000000
VERSION     DATDMP010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196576"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VI195_09175"
     CDS             complement(<1..831)
                     /locus_tag="VI195_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6824600.1"
                     /translation="MAAHSDENKRSPLKAWVRALERTATIERDRSLTLPVLIGRLAER
                     FGEAPALVSTDASLSYRQLAAACHRYARWGLAHGLSRGDTVCLVMANCPDYLAIWLGF
                     ARIGATVALINTNLRGELLAHSLKLVGPRYVVAGGSLSAAVRAVRPLLAADVECWVSG
                     SGGADLPRLDEAIAELPGDPLHPQEVPSAPALEDRALYIYTSGTTGLPKAANVSHYRL
                     MQWSHWFAGLIDVQPDDRMYDCLPMYHSVGGVVATGAVLVNGGSVVIRDKFSTSRFWD
                     D"
     gene            881..2185
                     /locus_tag="VI195_09180"
     CDS             881..2185
                     /locus_tag="VI195_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEY6824601.1"
                     /translation="MRPETIALHAGYDCDPATKAVAVPIYQTVAYSFDSAAHGAALFD
                     LEVEGFRYTRIGNPTGAVLEQRLAELEGGIDALAVSSGQSAMYYTVLNLIEPGRNLVS
                     VPQLYGTTHTLFAHILPRFGIKVKFGRTDSPQELESLIDGNTRALFCETVGNPAGNIC
                     DLEALAAIAQRARVPLVVDNTVPTPILLRPIEYGADIVVHSLTKFLGGHGTTLGGAVI
                     DSGRFRWEQLGNRYPMFTEPDPSYHGLIYTKHYGDKAFVGRMRSVFQRTLGAVLSPFN
                     AFLLMQGIETVALRVERHVENARRVAEFLRADARVDSVSYVGFPDNPYYPLCQKYLGG
                     RACSLLTFAIRGGFDAGTRFYDALRLFKRLVNLGDAKSLACHPASTTHRQMSPEEQRQ
                     AGIGPETIRLSVGIEHIDDIIADLDQALAAATRPKVRQAATT"
     gene            complement(2175..2459)
                     /locus_tag="VI195_09185"
     CDS             complement(2175..2459)
                     /locus_tag="VI195_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY6824602.1"
                     /translation="MSSEPLPTPANPAIRDRLLHLVGQILGKPEAASSLPPEARLSEL
                     GMSSIKMVNLMLAVETEFDLTIPQGDITPDNFRSVATVEALLARLLAVRS"
     gene            complement(2456..>2720)
                     /locus_tag="VI195_09190"
     CDS             complement(2456..>2720)
                     /locus_tag="VI195_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824603.1"
                     /translation="GAQPHYPTLPHGYLSAEGTALLMEFRERALAERNAALFQSFPFA
                     PVARVLHNRWRAAGVALYRNWLDFILEARAAGRVSETVSLTRP"
BASE COUNT          491 a          899 c          880 g          450 t
ORIGIN      
        1 gtcatcccag aagcggctcg tcgagaactt gtcacggatc acgaccgagc cgccgttgac
       61 cagcacggcg ccggtcgcca ccacgccgcc gacgctgtga tacatcggca ggcagtcgta
      121 catacggtca tcgggctgca catcgatcag accagcgaac cagtgactcc actgcatcag
      181 ccggtagtga ctgacgttcg ctgccttggg gagcccggtg gttcccgagg tgtagatgta
      241 cagcgcccga tcctcgagtg cgggggctga gggaacttcc tgcgggtgca gcgggtcacc
      301 gggcaactcg gcgatcgcct catcgaggcg cggcaggtcc gcgccgcccg agccgctcac
      361 ccagcattcg acatcggcag caaggagcgg acgcaccgcg cgcaccgccg cgctgagcga
      421 gccacccgca acgacataac gcggtccgac cagcttcagc gaatgggcga ggagctcacc
      481 gcgcaggttg gtgttgatga gcgcgacggt ggcgccgatc cgcgcaaagc ccagccagat
      541 cgccaggtaa tcggggcagt tcgccatcac cagacatacg gtatcgcccc gcgagagccc
      601 gtgcgccagc ccccagcgcg catagcggtg gcaggccgca gcgagctgcc gatagctgag
      661 tgacgcgtcg gtggaaacga gtgcgggagc ctcgccgaaa cgctcggcca ggcgcccgat
      721 caggacgggc agcgtcagcg agcggtctcg ctcgatcgtc gcggtgcgtt cgagggcgcg
      781 tacccatgcc ttgagaggcg atcgcttgtt ttcgtcgctg tgcgctgcca tcgtgccggc
      841 attctatgat aggcgagaca agagcgaccc gggatggccc atgagacctg aaaccatcgc
      901 cctgcacgcg ggctacgact gcgatccggc caccaaggcg gtggccgtgc cgatctacca
      961 gacggttgcc tattcgttcg acagcgccgc gcacggcgcc gcgctgtttg acctggaggt
     1021 cgaaggcttc cgctacaccc gtatcggcaa tccgacgggc gcggtgctcg agcagcgcct
     1081 cgccgagctc gaaggtggga tcgatgcgct ggccgtgagc tccgggcagt cggccatgta
     1141 ctacacggta ctcaacctga tagagcccgg acgcaatctg gtctcggtgc cgcagctcta
     1201 tggcacgacg catacacttt ttgcccacat cctgccgcgc ttcggcatca aggtgaagtt
     1261 cggccgcacc gacagcccgc aggagctcga gtcactgatc gatggcaaca cgcgggcgct
     1321 attctgcgag acggtgggaa atccagccgg caacatctgc gatctcgagg cactcgccgc
     1381 catcgcccag cgagcccgcg tcccgctggt ggtcgacaac accgtgccga caccgatact
     1441 gctgcgaccg atcgagtatg gcgccgacat cgtcgtgcat tcgcttacga agttcctcgg
     1501 tgggcacggc acgacactcg gcggtgcggt tatcgacagc ggccgcttcc gctgggagca
     1561 gctcggcaac cgctacccga tgttcaccga gcccgatcct tcctatcatg ggctcatcta
     1621 caccaagcac tacggcgaca aggcgttcgt cggacgcatg cgcagtgtgt ttcagcgcac
     1681 cctgggcgca gtgctctcac ccttcaacgc cttcctgctg atgcagggca tcgaaacggt
     1741 ggcgctgcgt gtcgaacggc acgtggagaa cgcgcgccgg gtcgcggagt tcctgcgcgc
     1801 cgatgcgcgt gtcgactcgg tgagctacgt cgggtttccc gacaatccct attatccgct
     1861 gtgtcagaaa taccttggcg gccgcgcctg ctcgctgctc accttcgcca tccgcggggg
     1921 gttcgacgcc ggcacgcgct tctatgacgc gctgagactg ttcaagcggc tggtgaacct
     1981 cggcgacgcg aaatcgctcg cctgccatcc cgcctccacg acgcatcggc agatgtctcc
     2041 cgaggagcag cgccaggcgg gcatcggtcc tgagaccatc cgcctgagcg tcggcatcga
     2101 gcatatcgat gacatcatcg ccgatctcga tcaggcgctc gccgcggcta cgcggccgaa
     2161 ggtacgacag gccgctacga cctgacggcc agcagccgcg caagcagcgc ttcgaccgtt
     2221 gccaccgagc ggaaattgtc gggggtgatg tcgccctggg gaatggtgag atcgaattcc
     2281 gtctccacgg cgagcatgag attcaccatt ttgatggagc tcatgcccag ctcgctcagg
     2341 cgagcctcgg gtggcaagga ggaggcagcc tccggcttgc ccaggatctg ccccaccagg
     2401 tgtagcagtc ggtcacgaat ggcagggttt gcgggggtag gcaagggctc agagctcatg
     2461 gccgcgtcaa tgataccgtt tccgacactc gccccgcggc gcgtgcctcg aggatgaaat
     2521 cgagccaatt gcgatagagc gctacgcccg ccgcgcgcca gcggttgtgc agcaccctcg
     2581 cgacgggtgc gaacggaaaa ctttgaaaaa gcgcggcatt gcgctctgcg agcgcacgct
     2641 cgcggaactc catgagcagc gccgtccctt cggcggagag gtagccatga ggcagcgtcg
     2701 ggtaatgcgg ctgcgctccg
//
LOCUS       DATDMP010000525         5369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2085058, whole genome shotgun sequence.
ACCESSION   DATDMP010000525 DATDMP010000000
VERSION     DATDMP010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085058"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1320)
                     /gene="thrC"
                     /locus_tag="VI195_09195"
     CDS             complement(4..1320)
                     /gene="thrC"
                     /locus_tag="VI195_09195"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY6824604.1"
                     /translation="MGEPFLFRSSRGEAPPCTFSAALLQGLAPDGGLYVPVRWPHLPP
                     AQFAGDSGLAELAARLLAPFLAEDPLAAQLPAIAGEALDFPAPLVPLDRDGRLAVLEL
                     FHGPTAAFKDFGARFLAACFARMRAGDNRTLTILVATSGDTGGAVAAAFHGRPGVEVA
                     VLFPKGLVSPTQERQLSCWGGNVRSLAVRGTFDDCQRLVKAAFRDGELTARRLLSSAN
                     SINLGRLLPQSSYYAAASLEVWRRFGEPAAFISPSGNLGNALACLWARRVGLPIGEVL
                     LAHNANRTVPDFLASGEWRPRPSVATLASAMDVGDPSNMERLRALYPQYEALRGALSA
                     CSVSDAEIRARIRAGYQEYGQTWCPHTATAAEAWERLTPARRRERRWVLVATAHAAKF
                     REIVEPLIGRELPMPQTLARLFARPLQCVEIDATLAALRAELLRES"
     gene            complement(1327..2268)
                     /locus_tag="VI195_09200"
     CDS             complement(1327..2268)
                     /locus_tag="VI195_09200"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY6824605.1"
                     /translation="MSAAMRERASAFAPASVGNVAIGFDILGFAIDTLGDRVTVWRRE
                     APGVEVSAVRGVAGEVPHAAQDNTAGRALLALQEAVNPPFGFRVEIDKGIPLGAGLGG
                     SAASAVGAVVAANALLPRPVSRLELLQFAMTGEAVASGARHADNIAPSLFGGLVLTVG
                     IDHPRVKQIPVPAAIRAVIVHPHMFLATAQARAILKLSVELSDFVWQTAHLAGFISGC
                     YTDDLDMIRASFEDVVIEPQRAALIPGFQEVRRGAMEAGALGCSISGAGPSMFAWTLE
                     ATAPAVLAAMRREFARHSLATDHWVVEVRSAGARLIE"
     gene            complement(2265..4766)
                     /gene="thrA"
                     /locus_tag="VI195_09205"
     CDS             complement(2265..4766)
                     /gene="thrA"
                     /locus_tag="VI195_09205"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEY6824606.1"
                     /translation="MVQPDPWVVHKFGGSSVADADCFRKVAAILEAAPAGRLAVVLSA
                     CRGVTDALLRLVALAERQDERVSAEVTQLRDRHVAIAEELLSPTGAQRFRAAFESDCR
                     DLKEVLRSLQLPRSLEHPRGSARDVSDLVSGYGEVWSTRLFQRFLAERARRAGPVAWL
                     DARRVVVVEWGPLGPGVQWPESRANLEAQVPAQFHGTLIITGFIAVDRRGVPTTLGRN
                     GSDFSASIFGALLGASEILIWTDVDGVLSADPRRVPDATVIDSLSYNEAMELAYFGAK
                     VIHPQTIAPAVSDRIPIWIRNTFAPQKAGTLICAEPASMLPVKGITSIERVALVNLEG
                     TGMIGVPGTAHRLFGALREEGISVILISQGSSEHSICCAIPQEQAERAAAVVTRAFER
                     ELEEGQIQSVDIDADQAILAVVGDGMAGTPGIAAKLFTALGDSGVNVRAIAQGASERN
                     ISAVVEGRSAMRALRAVHAGLYLSPHTLSIGVIGPGTVGRVLLDQLAAQSEHLRQQFR
                     LDLRVRGILGSQRMLLADTNIPMQDWRGQYQQSTRAADIERFVEHVRLDYLPHTVLVD
                     CTASAEIAAHYADWLADGIHIVTPNKKANSGDYAYYQRLQSARRSGASHYLYEATVGA
                     GLPIVQTLRDLVQTGDEISGIEGIFSGTLAYLFNIYDGRRGFAQIVQDARARGFTEPD
                     PREDLSGQDVARKLIILGREMGLPLELRDVQIETLVPAQLAGGSIEEFMVQLPQHDAA
                     MARRLAAAQARGKVLRYTGKLSASGTATVGLQELDAHHAFANIALTDNVVRFATRRYR
                     ENPLIVQGPGAGPEVTAGGVFADLLRLAAYLGARL"
     gene            complement(4808..>5369)
                     /locus_tag="VI195_09210"
     CDS             complement(4808..>5369)
                     /locus_tag="VI195_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6824607.1"
                     /translation="AAAGVNFAMIRYYFGGKPGLYREILQGLLPAQLADIEAWSRGGA
                     GQLEELLANMVRTWAAHPWIAGFVLREVLLPGGPMRAMFLREFPERFAPCIERAVVEQ
                     MRRGSVRADLDPKLLVLSIVSLAIFPFLGFALTSRVFGVRHDEEFVARFIAHTQALLA
                     RGVGAAHPDGARANVHAVVAHARERQ"
BASE COUNT          900 a         1890 c         1835 g          744 t
ORIGIN      
        1 cacctagctc tccctcagca gctccgcgcg cagcgctgcg agcgtcgcat cgatttcaac
       61 acactgtagc gggcgtgcga ataggcgcgc cagcgtctgc ggcatcggca gctcgcgccc
      121 gatcagcggc tcgacgatct cgcggaactt cgccgcgtgg gcggtcgcca ccagtaccca
      181 gcgccgctcg cggcgccgtg ccggcgtcag tcgctcccag gcctcggccg ccgtcgcggt
      241 atgcgggcac cacgtctgcc catactcctg gtaaccggcg cggatgcgcg cgcgaatctc
      301 cgcatcgctt accgagcagg cgctcagcgc gccgcgcaac gcctcgtatt gcggatagag
      361 cgctcgcaag cgctccatgt tgctcgggtc gccgacatcc atcgcggaag cgagcgtggc
      421 gacactcggg cgcgggcgcc actcaccgct cgcgagaaag tcgggcacgg tgcgattcgc
      481 gttgtgcgcg agcagcacct cgccgatcgg cagccccacc cggcgcgccc acagacacgc
      541 gagcgcattg ccgagattgc cgctcggact gatgaaggcc gcgggctcgc cgaaacggcg
      601 ccacacctcg aggctggccg cggcgtaata gctgctctgc ggcagcaggc gcccgagatt
      661 gatgctgttg gccgaggaca ggaggcgccg tgccgtcagc tccccatcgc ggaacgccgc
      721 cttcaccagg cgctggcagt catcgaacgt gccccgcacc gcgagcgagc ggacgttgcc
      781 gccccagcag ctcagctgtc gctcctgcgt gggggagacg agccccttgg gaaacagtac
      841 cgccacttcc actccgggcc ggccgtggaa agcggcggcg accgcaccgc ccgtgtcccc
      901 cgaggtcgct accaggatcg tgagcgtgcg gttatcgccg gcgcgcatgc gcgcgaagca
      961 ggcggctaga aagcgcgcgc cgaagtcctt gaatgccgcg gtcggtccgt ggaagagctc
     1021 gagcaccgcc agccggccgt cccggtcgag gggtacgagc ggtgcgggaa aatccagtgc
     1081 ttcgccggcg atcgcaggca gctgcgcggc gagcggatcc tccgccagaa acggagcgag
     1141 gagcctcgcg gcgagctcag caagcccgga gtcgccggcg aactgcgccg gaggcagatg
     1201 aggccagcgc acgggcacgt agagcccgcc atcgggagcc agcccctgca gcagcgccgc
     1261 actgaaggtg cacggtggcg cctccccgcg cgaactccgg aaaagaaacg gctcgcccat
     1321 gcgccctcac tcgatgagtc gtgcgcccgc cgagcgcacc tcgaccaccc agtggtcggt
     1381 ggcaagtgag tgacgcgcga actcgcgccg catggccgcg agcaccgcgg gcgccgtcgc
     1441 ctcgagcgtc caggcaaaca tcgaagggcc cgcgccggag atcgagcagc cgagcgcacc
     1501 ggcctccatc gcgccgcgcc gcacctcctg gaacccgggg atgagcgcgg ctcgctgtgg
     1561 ctcgatcacc acgtcctcga aggaggcgcg gatcatgtcg agatcgtccg tgtagcagcc
     1621 ggagatgaag ccggcgagat gagcggtctg ccagacgaag tccgacagct cgacgctcaa
     1681 cttgaggatc gcgcgtgcct gggccgtggc gaggaacatg tgcgggtgga cgatgaccgc
     1741 gcggatcgcg gccggaaccg gaatctgttt cacgcgcgga tggtcgattc ccaccgtcag
     1801 cacgagaccg ccaaagagcg atggcgcaat gttgtccgcg tgccgcgcgc cgctcgctac
     1861 cgcctctccg gtcatcgcaa actgcagcag ctcgaggcgg ctcaccgggc gcggcaacag
     1921 ggcgttggcg gctaccactg cgccgaccgc agaggccgcg gaaccgccga ggccggcacc
     1981 gagcgggatg cctttgtcga tctcgacccg gaagccgaaa ggtggattca ccgcctcctg
     2041 cagcgcgagc aacgcccgtc cggcggtgtt gtcctgcgca gcgtgcggca cctcgccagc
     2101 gacgccgcgc accgcgctca cttcgacgcc cggcgcctcg cgccgccaga cggtaacgcg
     2161 atcgccgagc gtatcgatgg caaagccgag aatgtcgaag ccgatcgcca cgttgccgac
     2221 ggaggccggt gcaaatgcgc tggcgcgctc gcgcatggcc gcgctcacag gcgcgctccc
     2281 aaataggcag ccagccgcaa caggtcggcg aacacgccgc cggcggttac ctccgggccg
     2341 gcgcccggcc cctgcacgat gagcgggttc tcgcgatagc ggcgcgtcgc aaagcgcacg
     2401 acattgtcgg tgagcgcaat gttggcgaac gcatgatgcg catcgagctc ctgcagaccg
     2461 acggtggccg tgccgctggc gctgagcttg ccggtgtaac gcagcacctt gccgcgtgcc
     2521 tgggcagcgg cgagccggcg cgccatggcg gcatcgtgct gcggcagctg caccatgaac
     2581 tcctcgatgc tgccgccggc gagctgcgcc ggcaccaggg tctcgatctg cacgtcgcgc
     2641 agctcgagcg gcaggcccat ctcgcgcccg aggatgatga gtttgcgcgc cacgtcctgc
     2701 ccggagagat cctcgcgcgg gtcgggctcg gtgaagccgc gcgcgcgcgc atcctgcacg
     2761 atctgcgcga agccgcgccg cccgtcatag atgttgaaca ggtaagcaag cgtgcccgag
     2821 aagatgccct cgatgccgct gatctcatcg ccggtctgca ccaggtcgcg caaggtctgc
     2881 acgatcggca gacccgcgcc gaccgtcgcc tcgtagaggt aatgcgaggc gccgctgcgc
     2941 cgcgcgctct gcaggcgctg gtagtaggcg tagtcgccac tgttggcctt cttgttggga
     3001 gtcacgatgt ggatcccatc cgccagccag tcggcgtagt gcgcggcgat ctccgcactg
     3061 gcggtgcagt ccaccagcac cgtgtgcggc aggtaatcga gccggacatg ctcgacgaag
     3121 cgctcgatgt ccgccgcgcg cgtgctctgc tggtactggc cacgccagtc ctgcatcggg
     3181 atgttggtat cggcgagcag catgcgctgc gagcccagga tgccgcgcac gcgcaggtcc
     3241 aaccggaact gctgccgcaa gtgctcactc tgggccgcca gctgatcgag cagcacacgg
     3301 ccgacggtgc ccgggccgat gacgccgatc gagagcgtgt gcggcgagag gtacagcccc
     3361 gcgtgcacgg cacgcagtgc gcgcatggcg ctgcggcctt cgaccacggc cgagatgttg
     3421 cgctcggatg cgccctgggc gatggcgcgc acgttgacac cggagtcgcc gagcgccgtg
     3481 aacagcttgg cagcgatgcc gggggttccc gccatgccat cgccgaccac ggcgagtatc
     3541 gcctggtcgg catcgatgtc gacgctctga atctgcccct cctcgagctc gcgctcgaag
     3601 gcgcgcgtca ccacggcggc ggcgcgctcg gcctgctcct gaggaatggc gcagcagatc
     3661 gagtgctcgg agctgccctg cgagatgagg atcactgaga tcccctcctc gcgcagcgca
     3721 ccgaacaggc gatgcgcggt gcccggcacg ccgatcatgc cagtgccttc gagattgacc
     3781 agcgcgaccc gctcgatgct ggtgatgccc ttgaccggca gcatcgaggc gggctcggca
     3841 cagatgagcg tgccggcttt ctgcggtgcg aaggtgttgc ggatccagat cggaatccgg
     3901 tcgcttaccg ccggcgcgat ggtctgcgga tggatgacct tggcgccgaa gtaggcgagc
     3961 tccatcgctt cgttgtagga aagcgagtcg atcaccgtcg catcgggcac acggcgcgga
     4021 tcggccgaca gcactccatc cacgtcggtc cagatgagaa tctcgctcgc gccgagcagg
     4081 gcgccgaata tcgaggcgga gaaatcgctg ccgttgcggc cgagggtggt cggcacaccg
     4141 cgccggtcga cggcaatgaa ccccgtgatg atgagtgtgc cgtgaaactg cgccggcacc
     4201 tgggcttcga ggttggcgcg ggactccggc cattgcacac ccgggccgag cggtccccac
     4261 tcgaccacca ccacgcgcct ggcatcgagc cacgcgaccg gacctgcacg gcgcgcgcgc
     4321 tcggccagga agcgctgaaa cagccgcgtc gaccagactt cgccgtaccc cgacaccagg
     4381 tcgctcacat cgcgcgccga gccgcgcggg tgctcgagcg agcgcggcag ctgcagcgag
     4441 cgcagcacct ccttcaggtc gcggcaatcg ctctcgaacg ccgcacggaa gcgctgtgcg
     4501 ccggtggggc tgagcagctc ctcggcaatc gccacgtggc gatcgcgcag ctgcgtgacc
     4561 tctgcactca ctcgctcgtc ttggcgttcg gcgagcgcga ccagccgcag cagcgcatcg
     4621 gtgacgccgc ggcaggcgga caacaccacc gccaggcgcc ccgcgggcgc agcttccagt
     4681 atggccgcga ccttgcggaa gcagtcggcg tcggcgacgc tcgacccccc gaacttgtgc
     4741 acgacccagg gatcgggctg gaccatttaa accagaggtt gacggagccg gaacaggccg
     4801 tgcgactcta ctgacgctcc cgcgcgtgcg caacgacggc gtgcacgttt gcgcgtgcac
     4861 cgtccgggtg cgccgcgccg acaccgcgtg cgagcagcgc ctgggtgtgg gcgatgaagc
     4921 gcgctacgaa ctcctcatcg tgccgcacac cgaacacccg gctggtcagc gcgaagccga
     4981 gaaaagggaa aatcgccaga ctgacgatgg acagcaccag cagctttgga tccagatccg
     5041 cgcgcacgct gccgcggcgc atctgctcca ccaccgcgcg ctcgatgcac ggcgcaaacc
     5101 gctcggggaa ctcgcgcaga aacatcgcgc gcattggccc accgggcagc agcacctcgc
     5161 gcagcacgaa accggcgatc cacgggtgcg ccgcccaggt gcgtaccatg ttggcgagca
     5221 gctcctcgag ctggccggcg ccaccgcgcg accaggcctc gatgtccgcg agctgcgcag
     5281 gcaacaaacc ctgcaggatc tcgcggtaca gaccgggctt gccgccgaag tagtagcgga
     5341 tcatggcgaa attgactccg gccgcggct
//
LOCUS       DATDMP010000526         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_973501, whole genome shotgun sequence.
ACCESSION   DATDMP010000526 DATDMP010000000
VERSION     DATDMP010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973501"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VI195_09215"
     CDS             complement(<1..1008)
                     /locus_tag="VI195_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY6824608.1"
                     /translation="MAEIAREILPVTLEGEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMRELGNDWNRAYKKSARVVGDVIGKYHPHGESAVYDTIVRLAQPFSMRYLL
                     VDGQGNFGSVDGDPPAAMRYTEVRMARIAHELLADIDKETVDFNPNYDESETEPSVMP
                     TRIPNLLVNGQTGIAVGMATNIPPHNLTETVNACVALLDDPTLPLVALMQHMPGPDFP
                     TGGIINGAQEIATAYRTGRGRLSVRARVLIEEVGRADRQAIVVTELPYQVNKARLIER
                     IAELVREKQLDGIAADGLRDESDKDGMRIVIELKRGEVTEIVLNNLYAHTPMETVFGI
                     NM"
     gene            complement(1139..1459)
                     /locus_tag="VI195_09220"
     CDS             complement(1139..1459)
                     /locus_tag="VI195_09220"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY6824609.1"
                     /translation="MPKYLVEAAYTADGLRGLQKDKASGRRQAMTKAVESLEGRVEAF
                     YFSMGDRDVVAIVEAPDAVSVAALSLAASATGLVRVKTTALLSVEEVDRALEKKHSYR
                     GPGT"
     gene            1523..>2075
                     /locus_tag="VI195_09225"
     CDS             1523..>2075
                     /locus_tag="VI195_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6824610.1"
                     /translation="MSSSGDAEFVIEPRWLLPVAPANAVLEAHAVAVGGGRILDVGPA
                     VELRHRYAGVMRIARERHALLPGLVNAHTRACHTLLRGLPVRGPRLRWLGEVRAPLER
                     RALGADLVRDGTRLGIAEMLRAGITCYADESPLPEEAARVAAAAQVRALIGLPVTDAA
                     DGWAQDAGAHLARAEALWDEYRSD"
BASE COUNT          318 a          670 c          710 g          377 t
ORIGIN      
        1 catgttgatg ccgaacacgg tctccatggg cgtgtgcgcg tacaggttgt tcagcacgat
       61 ctcggtcact tcaccgcgct tgagctcgat gacgatacgc atgccatcct tgtcggactc
      121 atcgcgcagt ccgtcggcgg cgataccatc gagctgcttc tcgcgcacca gctcggcgat
      181 gcgctcgatg aggcgcgcct tgttcacctg gtagggaagc tccgtgacca cgatcgcctg
      241 gcgatcagcg cggcccacct cttcgatgag cacgcgcgcc cgcaccgaca gacgcccgcg
      301 tccggtgcga taggcggtgg cgatctcctg tgcgccgttg atgatgccgc cggtcggaaa
      361 gtcggggccg ggcatgtgct gcatcagcgc cacgagcggc agcgtcggat catcgagcag
      421 ggccacacag gcgttcacgg tctcggtgag gttgtgtggc ggaatgttgg tggccatgcc
      481 gacggcgatg ccggtttggc cgttcaccaa caggttgggg atgcgcgtcg gcatcaccga
      541 gggctcggtc tccgactcgt cgtagttggg attgaaatcg acggtttcct tgtcgatgtc
      601 cgccagcagc tcgtgcgcga tgcgcgccat gcgcacttcc gtgtagcgca tcgcagccgg
      661 tggatcgcca tccaccgagc cgaagttgcc ctggccgtcg accagcaggt agcgcatgga
      721 gaaaggctgc gcgaggcgca cgatggtgtc gtacaccgcg ctttcgccgt gcgggtgata
      781 cttgccgatc acatcgccca ccacgcgcgc ggacttcttg tacgcgcggt tccagtcgtt
      841 gccgagctcg cgcatggcgt agagcacgcg ccggtggacg ggcttgaggc catcgcgcac
      901 atcgggcagc gcacgcccga cgatcacgct catggcatag tcgagatagg actgccgcat
      961 ctcgccttcg agagtgaccg gcaggatttc gcgggcgatc tcagccatca atcaggactt
     1021 gagtgtcagc gcccggctgg gcgcgaaata aaggcgaaag tttagcatga actagacctg
     1081 cggcggcctg tggatacagg ccaccggagg gaaacagcaa gggccgagag gtccggcgtc
     1141 aggtgcccgg accgcgatag ctgtgctttt tctcgagtgc gcgatcgact tcttcgacac
     1201 tgagcagtgc ggtggtcttt acccgcacga gcccggtcgc tgacgccgcg agcgagagtg
     1261 ccgcaacgct cactgcatcg ggtgcctcca cgatcgcgac cacatcccga tcgcccatgg
     1321 agaagtagaa agcctcgacc ctgccctcga ggctctctac cgctttcgtc atggcctggc
     1381 ggcgcccgct cgctttgtct ttctgcagac cgcgcaggcc atcggccgta taggccgctt
     1441 cgacgaggta cttaggcatg gtgcctcctt cgcgcggatc gtgctgcgcg ctgccgattg
     1501 tacgccgata tactccgctg ccatgtcgag ctctggcgac gccgagtttg tgatcgagcc
     1561 gcgctggctg ttgccggtcg cgcccgccaa tgcggtgctc gaggcacacg cggtcgccgt
     1621 gggcggcggg cgcattctcg atgtaggacc ggcggtggag ttgcggcacc ggtatgccgg
     1681 ggtgatgcgc atcgcgcgcg aacggcacgc attgctgccc gggctcgtga atgcgcacac
     1741 ccgcgcctgc cataccttgc tgcgcggact gccggtgcgc ggtccgcggc tgcggtggct
     1801 cggcgaggtc cgcgcgccgc tcgagcggcg cgcgctcggc gcggatctgg tacgcgatgg
     1861 cacgcggctc ggtatcgccg agatgctgcg tgcaggcatc acctgttatg ccgatgagag
     1921 tcccctgccc gaggaggcgg cgcgcgtcgc cgccgccgcg caggtacgcg ccctcatcgg
     1981 cctgccggtc acggacgcgg ccgatggctg ggcgcaggat gccggcgcgc atctggcgcg
     2041 cgcggaggcc ctctgggacg aataccgcag tgatg
//
LOCUS       DATDMP010000527         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_529596, whole genome shotgun sequence.
ACCESSION   DATDMP010000527 DATDMP010000000
VERSION     DATDMP010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529596"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..670)
                     /locus_tag="VI195_09230"
     CDS             complement(20..670)
                     /locus_tag="VI195_09230"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEY6824611.1"
                     /translation="MTPFPTPPAVLPPFDLSGGHVALDLVNSLDHRFGGEGPADRLER
                     YGELLRFAEQTGLLDRAGARRLERSVDAGAGTRAVRSARELREALAATLYGRIDGRAP
                     PAGALATLERCFQDAQRHRELRWRRTGGPAALEWQWGRAEDEAELPVWMVARAAEELL
                     TSQGLERVRACDVPTCRWLFLDTSRNHSRRWCNMKVCGNRMKARRFQERRSGHTQH"
     gene            749..1729
                     /locus_tag="VI195_09235"
     CDS             749..1729
                     /locus_tag="VI195_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY6824612.1"
                     /translation="MPAGMPAALRRLDIRVLAAFAAIYFLWGATFLAIRIAVLEVPPF
                     FASGLRFVVAGAALYSFMRLRGQPAPTPIEWRSIVLIALCMFVVTYAALFWAEQYVPS
                     GVTSVLEAALPVITVTLEVFVFRQQPFCWRTAFAVTLGFAGVAWLLLGTDAGTFPALP
                     CVAILAGATAWSLGAVLTRSLTRPRSLPLAAGAQMLVGGVLLLALSETTGELASLPHV
                     SLRAGAALAYLVVAGSLAGFTAYVWLLGRMPASRVASHAYVNPLVAVALGYFAAGETF
                     TPGMIMASALIVLSVVFILTAPRDRKSRLVPGSRSNGSTAGMACTDTAGS"
     gene            1683..2000
                     /locus_tag="VI195_09240"
     CDS             1683..2000
                     /locus_tag="VI195_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824613.1"
                     /translation="MAVLREWRALIRRDRKDEYLAYVRATGLAAYFATPGNLAASVAV
                     RDLDAERSEILTLSWWTDFAAIRAFAGEDFERARYFPEDDRFLLTRPECVLHYDATAP
                     AAR"
BASE COUNT          263 a          743 c          680 g          354 t
ORIGIN      
        1 gctctccgtt cgcagttcgt cagtgctgcg tatgcccgct gcggcgctcc tggaagcggc
       61 gcgccttcat gcggttcccg cacaccttca tgttgcacca acggcggctg tgattcctgc
      121 tcgtatcgag gaacagccag cggcaggtcg gcacgtcgca ggcgcgcacc cgctcgaggc
      181 cctgcgaggt gaggagttcc tcggccgcgc gcgccaccat ccacaccggc agctcggcct
      241 catcctcggc acggccccac tgccattcga gcgcggccgg tccgcccgtg cgccgccagc
      301 gcagctcgcg gtgccgctgc gcgtcctgaa agcagcgctc gagggtggcg agggcgcccg
      361 ccgggggcgc gcggccgtcg atccgtccgt agagcgtcgc cgccagcgcc tcgcgcagct
      421 cccgggccga gcgcaccgcg cgcgtacccg cacctgcatc gaccgagcgc tcgaggcggc
      481 gcgcaccggc gcggtcgaga aggcccgtct gctcggcaaa ccgcaacagc tccccgtagc
      541 gctcgagccg atccgcgggg ccctcgccgc cgaagcgatg gtcgagcgag ttgacgagat
      601 cgagcgccac gtgccccccg gacagatcga acggaggcag cacggccggc ggggtcggga
      661 atggggtcat aacctctgaa tatcatttta caggttacaa acgccgtgtc caggagtagc
      721 ataacctgct atgaacgttt cacaggttat gcccgccggg atgcccgcgg cgctgcgccg
      781 cctcgacatc cgggtgctcg ccgccttcgc ggcgatctat ttcctctggg gcgcgacctt
      841 cctcgcgatc cgcatcgcag tgctcgaggt gccgcccttc ttcgccagcg gactgcgctt
      901 cgtggtcgcg ggcgccgcgc tctactcctt catgcggctg cggggacagc ccgccccgac
      961 cccgatcgag tggcgcagca tcgtgctcat cgcgctctgc atgttcgtcg tcacctacgc
     1021 cgcgctcttc tgggcagagc agtacgtgcc ttcgggagtc acctcggtgc tcgaggcggc
     1081 gctgcccgtc atcacggtga cgctggaggt gttcgtgttc cgccagcagc ccttttgctg
     1141 gcgcacggcg ttcgcggtga ctcttggctt cgccggagtg gcgtggctgc tcctcggcac
     1201 cgacgccggg accttcccgg cgctgccatg cgtcgcgatc ctggccggtg ccaccgcctg
     1261 gtcgctcggc gcggtgctca cccgttcgct gacgcgcccg cgctcgctgc cgctcgctgc
     1321 cggcgcgcag atgctggtgg gcggcgtgct gctgctcgcc ctctccgaga cgaccggcga
     1381 gctcgcttcc ctgccgcacg tttcactgcg cgccggggcc gcgctcgcct acctcgtcgt
     1441 cgcgggatcg ctcgccggct tcacggcgta cgtgtggctc ctcgggcgca tgcccgcgag
     1501 ccgcgttgcg agccacgcct acgtgaatcc gctcgtggcg gtggcactcg ggtactttgc
     1561 ggccggcgag acgtttactc cgggcatgat catggcttcg gcgctcatcg tgctgagcgt
     1621 cgtcttcata ctgaccgcac cacgggaccg caagagtcgg ctggtccccg ggagtcgcag
     1681 caatggcagt actgcgggaa tggcgtgcac tgatacggcg ggatcgtaag gacgagtacc
     1741 ttgcctatgt cagggcgacg ggtctggccg cctatttcgc cacgcccgga aacctcgcgg
     1801 cgagcgtcgc ggtgcgcgac ctcgatgccg agcgctccga gattctcacc ctgagctggt
     1861 ggacggattt cgcggcgatc cgcgccttcg cgggcgaaga cttcgagcgg gcacgctact
     1921 ttcccgaaga cgatcgcttt ctgctgaccc gcccggaatg cgtgctgcac tacgatgcca
     1981 ccgctcccgc tgcgcgctga gcggccggac cgcaatgcgc cgccaccggc cctgcgaagc
//
LOCUS       DATDMP010000528         2060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1419656, whole genome shotgun sequence.
ACCESSION   DATDMP010000528 DATDMP010000000
VERSION     DATDMP010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419656"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1993
                     /locus_tag="VI195_09245"
     CDS             <1..1993
                     /locus_tag="VI195_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6824614.1"
                     /translation="SNTSLMILPENAPFRIGDWRIDPALDEISCDGRTIKLEPRTMCV
                     LVCLARRAGEVVSVDDLLDAVWKDVVVTQYSVYQAVAALRRSFGDDPKAPTYIDNVPR
                     RGYRLLAPVAVPATSPPAEAAAEPALAERSTTVVIPAHAQSGTAGIIRAPVPASWSLK
                     EGVLVAVALVIFATAAAYLAIGKLRISKSAVSSPTVAASPTPPAFSPPAHSIAVLPFV
                     NMSGDKEQEYFSDGLTEELLNSLAEINELQVAARTSAFSFKGKDTDIGTIARKLNVGA
                     VLEGSVRRSAHTIRITAQLINAVTGFHLWSKTYDRDLGDVLKLQTEIATAVAGALKVT
                     LLGDVAAKVELGGTRNPAAFDAYLRGAKAVSSIRDTKDLPAAIAAYTEAIRLDPHYAL
                     ALAARSIALSGVAQEAEPAAAAREGFDKAQADARQALALAPDLAQGHLALAYVSEDTL
                     DFTQAGEAYERALALAPGNAEVSRRSGRFAALIGHFDAGVAATRRAVVLDPLARGSHV
                     ALGDALYAARRYQEAVAAFAEVISLDPDFKATYGERGLAYYGLGDLKSARASCETKPD
                     HWVSQWCLAVIYDKLGRHADAEAVLAKLTAAFGDAPAYQDATIYAQWGNRAQALEWLE
                     TALRLRDSGLEYLKTDPLLDPLRKEPRFQAIERELRFPS"
BASE COUNT          356 a          673 c          670 g          361 t
ORIGIN      
        1 gtcgaataca tcgctgatga tcctgccgga aaacgcgccc tttcgaatcg gcgactggcg
       61 gatagaccca gcgctggacg aaatctcgtg cgacggcagg accatcaagc tggaaccgcg
      121 caccatgtgc gtcctggtct gcctcgcccg ccgtgccggg gaggtggtga gcgtcgacga
      181 ccttctcgat gccgtctgga aggacgtcgt cgttacccag tattccgtat atcaggcagt
      241 ggcagcgctc cgccgctcgt tcggcgacga ccccaaggca cctacgtaca tcgacaacgt
      301 gccgcgccgc ggctaccgcc tccttgcgcc cgtggctgtg ccggcaacct cgccacctgc
      361 ggaagcggct gcggagcctg ctctcgcgga gcgatcgact acggtcgtga ttcctgcgca
      421 tgctcagtct gggaccgcag ggatcatccg agcgcctgtg ccggcgtctt ggtcgctaaa
      481 ggaaggcgtg ctcgtagcag tggcgctggt gatcttcgcg acggcggcgg cctatctcgc
      541 catcgggaag ctccggatct cgaagtccgc cgtgtcgtca ccgacggttg cagccagccc
      601 gacgcctcct gccttcagcc cgccggcgca ctcgatcgcg gtgctcccat tcgtcaacat
      661 gagcggggac aaggagcagg agtacttctc cgacggactt acggaggagc tgctcaactc
      721 gctggctgag atcaacgagt tgcaggtcgc ggctcgcacc tcggcgttct cattcaaggg
      781 caaggacacc gacatcggca cgatcgcgcg caaactgaac gtgggcgcag tcctcgaagg
      841 gagcgtgcgg cgctccgcgc acaccatccg catcaccgcg cagctgatca atgccgtcac
      901 gggctttcac ctgtggtcga agacctatga ccgggactta ggggatgtgc tgaaacttca
      961 gaccgagatt gccaccgcgg tggccggagc gctgaaagtc acgctactcg gcgatgtcgc
     1021 ggccaaagtc gagctcggcg ggacgcgtaa ccccgccgcc ttcgatgcgt atctgcgcgg
     1081 ggccaaagcg gtgagctcca ttcgcgacac caaagacctc cccgccgcga ttgccgcata
     1141 cacggaagcg attcgtctcg atccgcacta tgcgctcgcg ctcgcagccc gatcgatcgc
     1201 cctttccggc gtcgcccagg aggccgagcc cgcagcggcg gcgcgtgaag gctttgacaa
     1261 ggcgcaggcg gatgcgcgcc aggcgctcgc gctggcgccg gatctggccc agggtcacct
     1321 cgcattggcg tacgtttcgg aagacaccct cgacttcacg caggcgggtg aagcgtacga
     1381 gcgcgctctt gcgctcgcgc cgggcaatgc agaggtctca cgaagaagcg gccgatttgc
     1441 agccctcata gggcacttcg atgcgggcgt tgcggccacc cgccgcgcgg tcgtgctcga
     1501 cccgctcgct cgcggaagcc atgttgcgct cggcgacgcg ctgtatgcgg cccgccgcta
     1561 tcaagaggcg gtggcggctt tcgcagaggt catcagtctt gaccccgact tcaaggcgac
     1621 ctacggggag cgcgggctcg cctattacgg gctcggggat cttaagagcg cgcgcgcctc
     1681 atgcgagacg aagccggacc actgggtgag ccaatggtgc ctcgcggtga tctatgacaa
     1741 gctcggccgg cacgccgatg cggaagctgt ccttgcgaaa ttgacggcag ccttcggcga
     1801 cgccccagcc tatcaggacg ccacgatcta tgcgcagtgg ggcaatcgag cccaggcgct
     1861 cgagtggctc gagacggcgc tgcggctgcg ggattcgggc ctggagtatc tgaagaccga
     1921 cccgctcctg gatcccttgc gcaaagagcc gcgcttccag gcgattgagc gggagttgag
     1981 gtttccgagt taagtggcgg tgcggttccg acgcaattca ttcggtccct cgaagattcg
     2041 ttccaatgtc cgctatgtag
//
LOCUS       DATDMP010000529         4645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2197421, whole genome shotgun sequence.
ACCESSION   DATDMP010000529 DATDMP010000000
VERSION     DATDMP010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197421"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VI195_09250"
     CDS             complement(<1..862)
                     /locus_tag="VI195_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824615.1"
                     /translation="MLLLALCTVTAEGQAAPADATQRVLEHHGNAARSGSYIVPGLTW
                     EAAARLRADPDFHAAIAGPVYTQPLYWTVPGTDHGLLLAATEQNLVYALDSRSGAVVW
                     KSTLGPAVPRSSLPCGNIDPVGITGTPALDERTQAVYIAAMVRDPASHGPRHLIFALS
                     LKDGSVVSGWPIDVEAALKRSGQTFHSSVQNQRGALTLAANTLYVPYGGHYGDCGDYH
                     GWVVSVSLQGVHAVRSWSTRARGGGIWAPGGVSSDGSALYIGTGNTMGAARWGDGEAV
                     IRLDADLNFAA"
     gene            1476..1718
                     /locus_tag="VI195_09255"
     CDS             1476..1718
                     /locus_tag="VI195_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824616.1"
                     /translation="MLVRVRQRIDAGRLPLFMPTTISAGYGTGAEVCQLCDLGISAEQ
                     TMYEVSDPRTSRLLGFHFACYVLWQRECSQRLASRK"
     gene            complement(1776..2069)
                     /locus_tag="VI195_09260"
     CDS             complement(1776..2069)
                     /locus_tag="VI195_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824617.1"
                     /translation="MKGPTLALLALAAASAALAQTTPPPTTAEPPASAAPEEQSQSPT
                     TPPPDSSSPNSADAQALMKDCMNQVQAANPNVPEKDIRNFCANEIAKSAQPRS"
     gene            complement(2300..3316)
                     /locus_tag="VI195_09265"
     CDS             complement(2300..3316)
                     /locus_tag="VI195_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY6824618.1"
                     /translation="MPASALAPIVLAIAAAALLLHELQIILLPFVIAAVTAYVCTPLV
                     ERLTAHTRLPRWLAALTVLVALMAAVALLGFVGVPPLARQVRAVSADLHGAVADFLRA
                     LMGPHSFPLLGSTLDAERLADLAVGGLQRGINGAQFMDLLRWLAAASFGFMLMWVLMG
                     YFLIDSHAIASGVLSLVPPRARPHLSRIWHELDPLLRRYFIGVALVVAYATAAAYLGL
                     GLVLGLHHALILALLTGLLEIIPMVGPVAAAVMAGLVAVQQAATSSDIWAYVGYAIAL
                     RLSIDQLIGPLVLGNAARVHPVVVIFCFLAGGALFGVVGMILAVPSALLVKVTLSVLY
                     RDAR"
     gene            complement(3379..4500)
                     /locus_tag="VI195_09270"
     CDS             complement(3379..4500)
                     /locus_tag="VI195_09270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY6824619.1"
                     /translation="MVAWLILRALRQNAVFVRIGARAAPEGCVLPSLTVIVPARNESA
                     NIGPCLRSLLAQQYPAQRLRFVVIDDDSQDDTAATVAALVRSDSRLTLLSAPPLPPGW
                     KGKVHACWYAVQAIPADTEWLCFIDADVRAQPRALTTAMQLAQAAALDLLSLAPRHEL
                     GSLAERLIIPCGLYVLGFSLDRARIQAPESGDALCTGQFMLMRRRAYEQVGGYERVYD
                     SICEDLDFARLLKRHGLKVRLEDGSAVLVTRMYTGWATLWPGFAKNLVDMLGGTGRTL
                     LTAASALVLSWAAVILPLIDAVACHGGARGECVALLPAALGSAAAFGLHFAGARHFGI
                     PWWYGITFPLGYTAGALIALDSVRRRLIHRVEWKGRRYA"
BASE COUNT          845 a         1512 c         1557 g          731 t
ORIGIN      
        1 gagcggcaaa gttgagatcc gcatcgagcc ggatcacggc ttccccgtcg ccccagcgtg
       61 cggcacccat cgtattgccg gtgccgatgt agagagcgct gccgtccgag ctgacacctc
      121 ccggcgccca gatgccgccg ccacgcgcac gcgtggacca actgcgcacg gcgtgcaccc
      181 cctgcaggga gacactcacg acccatccgt ggtaatcgcc gcagtcgccg tagtgtccgc
      241 cgtacggcac atagagtgtg ttcgcggcga gcgtgagtgc gccgcgctgg ttctgcaccg
      301 aggaatggaa tgtctgcccg ctcctcttca gtgccgcttc cacatcgatc ggccaaccgg
      361 agaccaccga gccatccttc agtgacagcg cgaagatcag gtgtctcggg ccatgggagg
      421 ctgggtcgcg caccatggca gcgatataaa ccgcctgcgt gcgctcatcg agtgccggcg
      481 tgccggtgat cccaaccgga tcgatgttgc cgcaaggtaa ggacgagcgc ggcacggccg
      541 gccccagtgt gctcttccac actacggctc cgctgcgcga atccagggcg tacacgaggt
      601 tctgctcggt ggcggccagc agcagtccgt gatcggtgcc cggcaccgtc caatagagtg
      661 gctgggtata cacgggcccg gcgatagcgg cgtgaaaatc cggatccgcg cgcaggcgag
      721 cggcggcttc ccaggtgagg cccggcacga tgtacgaccc ggagcgcgca gcatttccat
      781 gatgctcgag cacccgttgc gtggcgtcgg ccggcgcggc ctggccctcg gcggtaaccg
      841 tgcatagcgc gagcaacagc atcgctgcgc gccggcatcg aagatatcgg ccggtcgtaa
      901 tggcaacgcc ccagctacgg ctagctcttt tcatctgtga gatgacgcaa ggaatgcggc
      961 cgaaaaccaa tggtaacgtc gcgcctgcat tgcaggggga gccgcccgca cgttcgtcga
     1021 ggcaggcggc ctgcgcgaaa caaaatgggg ggttccacga tgattcagac aaggcatcga
     1081 aacgaatccg cacacggcag atcgatgtct cgcggatgct gctccaagtc ctgcttaccc
     1141 cgtcgcgatg acttcgagtt ttcgggctgg tgtatcagat cggcgtgggc gcgcggcgcg
     1201 gggaagcgcg tcggcgcttc ggcgagtcgt ctcaaggcgg cacgctagac cgcgctcact
     1261 gtcttcctgt ccgcgtattc ctgtcgggat tctctcaccc gtgcgagcgg cctcgcggcg
     1321 agctgcctca tggtgcgcgg caacacgctg agtccttgcc gtagttggtc gttcatcggc
     1381 tgttcaacgg tgagggtgga cagtatgcac atccgtttca acgcgggggc atcaggagcc
     1441 atatgcctac tgcccccgtt ttgtccgaaa gtgaattgct cgtgcgcgtc cggcagcgca
     1501 tcgatgcggg acgattgccg ctgttcatgc ccaccaccat cagcgccggc tatggcaccg
     1561 gggctgaggt ttgccagctc tgcgatcttg gcatctctgc ggagcagacg atgtacgagg
     1621 tcagcgaccc gcgaacctcc aggctgctcg ggtttcattt tgcctgttac gtgctttggc
     1681 agcgcgaatg ctcgcaacgc ctcgcgagcc gcaaatagca tcgcgcccac acggcgggcg
     1741 caatcggccc gccgtgtggg tgttggttac cgcactcagc tcctcggctg cgcagatttg
     1801 gctatctcgt tagcgcagaa gtttctgatg tccttctccg gcacgttcgg gttagcggcc
     1861 tgcacctgat tcatgcagtc cttcatcagc gcctgcgcat cggcactgtt ggggctgctg
     1921 gagtctggtg gcggcgtggt cgggctctgg ctctgctcct ccggagccgc gctggctggg
     1981 ggttctgccg tggtgggcgg cggggtcgtc tgcgccagtg cggcgctggc agccgcgagc
     2041 gcaagcagag ccaatgttgg tcctttcatt ggacacctcc tgtgatgatg caccgggcag
     2101 actaggcacc gtcggcgcat cgaagctatc tcccgattgc tgctgtcagc cgtcggcgat
     2161 gacctacgaa aggagttgcc cgcggtttgg agtgcattcc cctctgacgg gtgtctgtgt
     2221 gcgccgacaa tcaaccggcg caacgccctt actgcacagc ggcacggcat cgagttgctg
     2281 cgcggcgccg cgcgccggat cagcgcgcgt cccgataaag taccgacaag gttactttga
     2341 cgaggagtgc gctcggcacc gccaggatca tcccaaccac gccgaagagc gctccacctg
     2401 cgaggaagca gaaaatcacc actaccgggt gcacgcgcgc ggcattgccg agcaccagcg
     2461 gcccgatcag ctgatcgatg gacagccgca gcgcgatggc atagccgacg tacgcccaga
     2521 tatccgatga ggtagccgcc tgctgcacgg ctaccagacc cgccattacc gccgcagcga
     2581 ccgggcctac catcggaatg atctcgagga gacctgtgag cagggcgagg atgagcgcgt
     2641 gatgcagccc gagcacgagc cctagaccga ggtaggcggc cgcagtcgca taagccacca
     2701 ccagcgcgac gccaatgaaa tagcggcgca gcaagggatc gagctcgtgc cagatgcggc
     2761 tgaggtgagg gcgcgcgcgc ggcggcacca gcgacagcac accgcttgcg atcgcatgtg
     2821 agtcgatcag aaagtaaccc atcagcaccc acatcagcat gaagccgaag gacgctgcgg
     2881 ctagccaacg cagcagatcc ataaactgcg cgccattgat gccgcgctgc agtccgccca
     2941 cggcaaggtc cgcgagacgc tccgcgtcca gagtcgagcc aagcagcgga aaggaatgtg
     3001 ggcccatcag ggcgcgcaga aaatctgcca ccgcaccgtg cagatcggcg ctcacggcgc
     3061 gcacctggcg cgcgagcggc ggcacaccga caaaaccgag cagtgcgacg gccgccatca
     3121 gtgcgaccag cacggtgagc gcggcgagcc agcgcggcag gcgtgtgtgc gccgtcaacc
     3181 gctcgacgag cggtgtgcat acgtaggcgg tcacggcggc gatcacgaat ggcagcagga
     3241 tgatctgcag ctcgtgcagc agcagcgccg ccgcagcgat cgccaacacg ataggcgcga
     3301 gcgcactcgc tggcatgaga gcgcgccagc gctcgcccgc acgttgcggt tcgcgatcgg
     3361 gtgcggggtg gggagcgctc atgcgtagcg gcgtcccttc cactcgaccc ggtggatgag
     3421 ccgccggcgg acgctgtcga gcgcaatgag cgcgccagcc gtgtacccga gtggaaaggt
     3481 gatgccatac caccagggga tgccgaagtg gcgcgctccg gcaaaatgca ggccgaaggc
     3541 cgccgccgag ccgagcgcgg ccggcagcag cgcgacgcac tctccccgtg cgccgccgtg
     3601 gcacgccacc gcatcgatga gcggcaggat cacggcagcc cacgacaaca ccaaggcgct
     3661 cgccgcggtg agtagcgtgc gccccgtgcc gccgagcata tcgacgaggt tcttggcgaa
     3721 tccgggccag agcgtcgccc agccggtgta catgcgtgtc acgagtaccg cgctgccgtc
     3781 ctccagacgc actttgagcc cgtgccgctt cagcagtcgc gcaaagtcca ggtcctcgca
     3841 gatcgagtcg tacactctct catagcctcc gacctgctcg tacgcgcggc ggcgcatcag
     3901 catgaactga ccggtacaca gtgcatcgcc actctcgggc gcctgaatgc gcgcacggtc
     3961 gagcgagaag ccgagcacgt agagcccgca ggggatgatc agacgctcgg cgaggctgcc
     4021 gagctcatgc cggggggcga gcgagagcag gtcgagggcg gcggcctgcg cgagctgcat
     4081 ggcggtcgtg agtgcgcggg gctgggcgcg cacgtccgca tcgatgaagc acagccactc
     4141 ggtgtcggca ggtatggcct gcacggcgta ccagcaggcg tgcaccttgc ccttccagcc
     4201 cggcggcagc ggcggcgcag acagcagcgt aagccgggag tcgctgcgga ccagtgcagc
     4261 gacggttgcg gcggtatcat cctgcgagtc atcgtcgatc acgacgaagc gcagccgttg
     4321 cgcagggtac tgctgggcaa gcagcgaacg caggcacggg ccgatattcg cgctctcgtt
     4381 gcgggcgggg acgatcacgg tgagcgaggg aagcacgcaa ccctcgggcg ccgcgcgcgc
     4441 cccgatgcgc acgaacacgg cgttctgccg cagcgcgcgc aagatgagcc acgcgaccat
     4501 ggcgaggaaa acgagcgagg cgatgagcag agggtcggct ttgtgcattg ttgacggcaa
     4561 ttatcgcacg cagctcaggc acgccatgcc aaccggcgaa ccgagcctgc agcgctgctg
     4621 ccacgcggtg attgacagcg cccca
//
LOCUS       DATDMP010000530         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2197477, whole genome shotgun sequence.
ACCESSION   DATDMP010000530 DATDMP010000000
VERSION     DATDMP010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197477"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VI195_09275"
     CDS             <1..1120
                     /locus_tag="VI195_09275"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY6824620.1"
                     /translation="ADRRLERSARTRVASSWACPENELSTAVEHRSLTMASIRQNAYP
                     DRLPDWRNLGVIARSLIAVNALLLAHSFAASRDLSGAAERFTHSALVIEPLLLASIVA
                     LYALSNWLTRLPYWGGFAAVTGLVTVLAAVEYFSAAGMLQDEAGRLAHWTVLAAVITA
                     ALLGYLRLVARAYSPALSEARLQALQARIRPHFLFNSLNAVLALIRRDPKRAERALED
                     LAELFRSLMSDARQFVRLADEIALLERYADLEQLRLGERLRITWEMDSAPSDALLPPL
                     VLQPLLENAVYHGVEPGTETSDVLVRIVLKGERILATIENPYAETQLQRSGNRMALEN
                     IRERLMLFFDAEARLETHADGKRYRVEIDLPYRTGKAA"
     gene            1117..1884
                     /locus_tag="VI195_09280"
     CDS             1117..1884
                     /locus_tag="VI195_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY6824621.1"
                     /translation="MSPLRVFIVDDEAPARERMKELLGDIAAELPTEVVGEARHGLEA
                     LEKIPHCRAQVVLLDIRMPGMEGMEVARHLCTVDPAPAIVFVTAHDKHAVQAFELNAL
                     DYLLKPVRAERLAAALRKALEAATPIGEQLARAAPGPREYISAAERNRIVLVPVRDIL
                     FLRAEQKYVTLRTREREHLIEESLIALEREFGDTFVRIHRNALVARAAIRGFERATAP
                     GEEEPHWLVVLDGLAERLPVSRRQWPLLRDLVAEKSG"
     gene            1881..>2201
                     /locus_tag="VI195_09285"
     CDS             1881..>2201
                     /locus_tag="VI195_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY6824622.1"
                     /translation="MKLVIATRRSRLALWQAEHVRTRLQASHPGLEVELLAMSTRGDE
                     LLETRLDEAGGKGLFVKELETALADGRAQLAVHSIKDVPAELPPGFHLAAITAREDPR
                     DAFVS"
BASE COUNT          343 a          753 c          743 g          362 t
ORIGIN      
        1 cgcggatcga cggcttgagc gaagcgcccg gaccagagtt gcttcttctt gggcatgccc
       61 ggaaaatgag ctttcgactg cggttgaaca ccgctcccta accatggcca gtataaggca
      121 aaacgcctac ccggaccgcc tgcccgattg gcgcaatctg ggcgtcatcg cacgctcgct
      181 gattgcggtc aacgcgctgc tgcttgcgca ttccttcgcg gcgagccgcg atctgtccgg
      241 cgctgcggag cgcttcacgc acagcgcgct cgtgatcgag cctttgctgc tcgcaagcat
      301 cgtggcgctc tacgcgctct ccaattggct tacgcgcctg ccctattggg gcgggttcgc
      361 cgcagtgacc gggctcgtga cggtccttgc ggccgtggag tatttctccg ctgccgggat
      421 gctgcaggac gaggccggcc gcctggcgca ctggaccgtc ctggccgccg tgattacggc
      481 ggccttgctc ggctacttga ggctcgtggc aagggcctat tcgcccgcgc tctcggaagc
      541 gcgcctccag gccctgcagg cgcggatccg tccgcacttc ctgttcaaca gcctcaatgc
      601 ggtgctcgcc ctgattcgcc gcgaccccaa gcgggccgag cgcgcactgg aggacctcgc
      661 ggagcttttc cgcagcctca tgtccgacgc gcgccaattc gtccgcctgg ccgacgagat
      721 cgcgctcctc gagcgctatg ccgatctcga gcagcttcgc ctgggagagc gcctgaggat
      781 cacgtgggaa atggatagcg cgccttccga cgcgctgctg ccgccgctcg tgctccagcc
      841 gctgctcgag aacgccgtct atcacggcgt ggagcccggc acggaaacga gcgacgtgct
      901 cgtccgaatc gtgctcaagg gcgagcgcat cctcgccacg atcgagaacc cctatgccga
      961 gacccagctg cagcgcagcg gcaaccgcat ggcgctcgag aacatccgcg agcgcctgat
     1021 gcttttcttc gacgcggaag cgcggctgga gacccacgcc gacggcaagc gctaccgcgt
     1081 cgagatcgac ctgccttacc ggaccgggaa ggcggcttga gcccgctgcg cgttttcatc
     1141 gtcgacgacg aagcgcccgc ccgcgagcgc atgaaggagc tcctgggcga catcgccgcc
     1201 gagctgccga ccgaggtcgt aggcgaggcg cgccacgggc tggaggcgct tgaaaagatc
     1261 ccgcactgca gggcgcaggt cgtgctgctc gatatccgca tgcccggcat ggaaggcatg
     1321 gaagtcgcgc gccatctttg cacggtcgat cccgcgcccg ccatcgtctt cgtcaccgcg
     1381 cacgacaagc acgccgtcca ggcgttcgag ctgaatgcgc tcgactatct gctgaagccg
     1441 gtgcgcgccg agagactggc ggcagcgctc aggaaggcgc tcgaggcggc tacgcccatc
     1501 ggcgagcagc tcgcgcgcgc cgccccgggg ccgcgcgaat acatctcggc ggcggagcgc
     1561 aaccgcatcg tgctcgtccc cgtgcgcgac atcctgttct tgcgggcgga gcagaagtac
     1621 gtcaccctgc gcacccgcga gcgcgagcac ctgatcgaag agtcgctgat cgcgctggaa
     1681 cgggagttcg gcgacacgtt cgtgagaatc catcgcaatg cgctggttgc ccgggccgcc
     1741 atccgcggct tcgagcgcgc tacggcgccg ggcgaggagg agccgcactg gctggtcgtt
     1801 ctggacgggc tggcagagcg gctgcccgtg agccggcggc aatggccgct cctgcgcgat
     1861 ctggtggcgg agaaatccgg atgaagctgg tcatcgccac gcgccgcagc cgccttgccc
     1921 tctggcaggc ggagcacgtg cgcacccggc tgcaggcctc gcatcccgga ttggaggtcg
     1981 agctgctggc catgtcgacg cgtggcgacg agctgctgga gacgcgtctg gacgaggcgg
     2041 gcggcaaagg gctcttcgtg aaggagctgg aaacggcgct cgcggatggc cgcgcccagc
     2101 ttgcggtgca ctcaatcaag gacgtgcctg ccgagctgcc gccgggattc catctcgccg
     2161 cgatcacggc gcgggaggat ccgcgcgatg cctttgtttc g
//
LOCUS       DATDMP010000531         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2086347, whole genome shotgun sequence.
ACCESSION   DATDMP010000531 DATDMP010000000
VERSION     DATDMP010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086347"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VI195_09290"
     CDS             <1..231
                     /locus_tag="VI195_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY6824623.1"
                     /translation="ELAAREWAVLEVLLGKVEKIVSKESIIQAVAGWDEELSPNAIEV
                     YISRLRGKLERAGIRIRTVRGFGYMLEEFKAG"
     gene            complement(201..1121)
                     /locus_tag="VI195_09295"
     CDS             complement(201..1121)
                     /locus_tag="VI195_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6824624.1"
                     /translation="MLNRAMSYVLGTLRMLSIGAFTGYLVAGAALWLYQRHLIFEPAR
                     EMQAAPRDFSFPVLDTTIALPAAASGTKAQLHGWWIAADRADARVVLYLHGNDGNVST
                     SMREIAPLRALGYAVFVIDYRGYGQSAGAFPSERSVYEDAEAAWHHLVADKGIDPRRL
                     YLYGHSLGGAVAIELATRHPEAAGLIVESSFTSIYDMARLEKPYKLFPVKLFLNQRFD
                     SIDKVERLALPVLFIHGTADEVVPFSMGEQLYRRSGGAKRFVAIEGGRHDDNAVAGGQ
                     AFLAALRNFVADTQRAPGAAAQPALNSSSM"
     gene            complement(1174..2082)
                     /locus_tag="VI195_09300"
     CDS             complement(1174..2082)
                     /locus_tag="VI195_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6824625.1"
                     /translation="MNPSSAIELAGGPHAALLFSGLAGSPLEVRFVGRLLHRAGFSVF
                     MPALRGYSMGHSGSEWPLWLTQAEDLYVRLCSQYKTVSVAGLSAGATLALALAVRQPA
                     LQSLALWSVTLHHDGWAIPWYHWLFRPGHYLGLGKYWAYRERTPFGLKNERWRARVAE
                     AMRSHKVSMAGPAEIPAEFLFQFTRLARYAERNLNKVRSDTLVLHAADDETASPRNAD
                     AVFAGIGSAHKRKILLGDSYHLITMDNERELVARETIRFFQQSIMHRHPEEHLQLVSS
                     ARALLRLQRRRAEERLGRPVSRAHYP"
     gene            2112..2600
                     /locus_tag="VI195_09305"
     CDS             2112..2600
                     /locus_tag="VI195_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY6824626.1"
                     /translation="MRTASTLPKIVLRNSRAWAPRTGVKWAAVVIAFLAALILRLALQ
                     DHLGPRYPLLFFTMVTLLVHFFYGLLPAIVLALVSLPTGIYLFVPPYVSFDLPTADDL
                     LRVVNYIVVTIIFMVLIQYLRRAQYQAVLLYEIAESRYLMLLDSEADRNAIEEETLGR
                     DG"
     gene            complement(2623..3060)
                     /locus_tag="VI195_09310"
     CDS             complement(2623..3060)
                     /locus_tag="VI195_09310"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY6824627.1"
                     /translation="MRAASFLVLLCVYGAAAAQAPTPVGLWKTYSDRTGEADGLVRVT
                     QANGELEGTVVKVFSPPAQSANPLCEECPGELRNQPIVGMKILWGMRPEADAYTGGEI
                     LDPDEGKVYRCTLRVVAGGRKLEVRGYIGISLFGRTQVWDRVD"
     gene            3114..>3546
                     /locus_tag="VI195_09315"
     CDS             3114..>3546
                     /locus_tag="VI195_09315"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6824628.1"
                     /translation="MLRLLLLPISAILVVSGVSAYYLALEPATEALDASLIDAGIAIR
                     ERIFVRDGLVTVDLPTAAEQVLRADRYDAVYYVVRDPGGNLIAGDSGIPLPPPGQQAQ
                     DNVMFYDAEFRGARVRGATLLAPCGKGPCAVTVAETTRKRDR"
BASE COUNT          592 a         1174 c         1181 g          599 t
ORIGIN      
        1 gagctcgccg cgcgcgagtg ggcggtgctc gaggtgctcc tgggcaaggt ggagaagatc
       61 gtctccaagg aaagcatcat ccaggccgtc gccggctggg acgaggaact ctcgccgaac
      121 gccatcgagg tctacatttc ccggctgcgc ggcaagctcg agcgcgcggg gatccgcatc
      181 cgcaccgtgc gcggattcgg ctacatgctg gaggagttca aggcgggctg agccgcggcc
      241 cccggcgcgc gttgcgtgtc ggcgacgaaa ttgcgcagcg cggcgaggaa ggcctggccg
      301 ccggccacgg cattgtcgtc gtgccgcccc ccctcgatgg cgacgaagcg cttcgccccg
      361 cccgagcgac ggtagagctg ctcccccatc gagaacggca cgacctcgtc cgccgtgccg
      421 tggatgaaga gcaccggcaa cgccaggcgc tccaccttgt cgatggagtc gaagcgctgg
      481 ttgaggaaga gcttcaccgg gaagagcttg tacggtttct cgagccgcgc catgtcgtag
      541 atcgaggtga agctcgattc cacgatcagc cccgccgctt cgggatgccg ggtggcgagc
      601 tcgatcgcca ccgcgccgcc aagggaatgg ccgtagagat aaagccgcct gggatcgatc
      661 cccttatcgg cgacgaggtg gtgccaggcc gcctccgcgt cctcgtacac gctgcgctcc
      721 gaaggaaacg cgcccgcgct ctgcccgtat ccgcggtagt cgatgacgaa cacggcgtag
      781 ccgagcgccc gcagcggtgc gatctcccgc atgctcgtgc tcacgttgcc gtcgttgccg
      841 tggaggtaga gcaccacgcg cgcgtccgcc cggtccgctg cgatccacca gccgtgcagc
      901 tgcgccttcg tcccgctcgc tgctgctggg agtgcgatcg tggtgtcgag gaccgggaag
      961 gaaaaatcgc gtggcgcggc ctgcatctcc cgcgccggct cgaagatcag gtggcgctgg
     1021 tagagccaca gcgcggcgcc cgccaccagg tagccggtaa acgcgccgat cgaaagcatg
     1081 cgcagcgtgc cgaggacgta gctcatagcg cggttcagca tgaccgaaca ctacgaagcc
     1141 taggttaagc gccgtccttg gcgcactagc ccattagggg taatgcgctc gcgatacggg
     1201 gcggccgagc ctctcttcgg cccggcgccg ctgcaggcga agcagcgcgc gcgcgctcga
     1261 cacgagctgc aaatgctcct cgggatggcg atgcatgatg ctctgctgga agaaacggat
     1321 ggtttcccgc gcaacgagct cgcgctcgtt gtccatggtg atcaggtggt agctgtcgcc
     1381 aaggaggatc ttgcgcttgt gcgcggagcc gatcccggca aaaacggcgt cggcgttgcg
     1441 agggctcgcg gtttcgtcgt ccgccgcgtg caagaccagg gtgtcgcttc ggaccttgtt
     1501 gaggttgcgc tcggcgtagc gggcgagccg ggtgaattga aacaggaatt cggccggtat
     1561 ttcggccggg ccggccatgg aaaccttgtg cgagcgcatg gcttcggcca cgcgcgcccg
     1621 ccagcgctcg ttcttaaggc caaaaggcgt cctttcgcga taggcccagt atttccccag
     1681 gccgaggtag tggccgggcc tgaatagcca gtgataccag gggatggccc agccgtcgtg
     1741 atgcagggtg accgaccaca gcgcaagcga ttgcagggcg ggctggcgga ccgcaagcgc
     1801 cagggcgagc gttgcgcctg cagagagacc ggcaacgctg acagtcttgt attgcgagca
     1861 caaacgcaca tagaggtctt ccgcttgcgt gagccagagc ggccactcgc tgccggaatg
     1921 gcccatgctg tacccgcgca gggccggcat gaagaccgag aatcccgcgc gatgcagcag
     1981 gcggcccacg aagcgcactt cgagcggcga gcccgccagc ccggaaaaaa gcagcgccgc
     2041 gtgcgggccg ccggcaagct cgatggcgct ggacggattc atgcgggcgc cgtagtgtgc
     2101 ggtgaaacgc aatgcgaacc gcttccaccc tgccgaagat cgtcctgcgc aacagccggg
     2161 cgtgggcgcc gcgcaccggc gtcaaatggg ccgcagtcgt cattgctttt ctcgccgccc
     2221 tgatcctgcg gctcgcgctg caggatcacc tcggcccgcg ctatccgctg cttttcttca
     2281 ccatggtgac gctgctcgtg cattttttct acggccttct tcccgcgatc gttctcgcat
     2341 tggtcagcct tccgaccggg atttaccttt tcgtgccgcc gtatgtgagc ttcgacctgc
     2401 ccaccgcgga cgacttgctg cgcgtcgtga actatatcgt agtcacgatc atcttcatgg
     2461 tgctgatcca gtacctgagg cgcgcgcagt accaggccgt actcctctat gagattgccg
     2521 agtcgcgcta cctgatgctc ctagactcgg aagccgatcg aaatgccatc gaagaggaaa
     2581 cgctcggccg cgacggctga atgcgcggct accggctcgc gtctagtcaa cccggtccca
     2641 cacctgggtg cggccgaata gggaaatccc tatgtagccg cgcacctcga gcttgcggcc
     2701 gccggcaacc acccgaagcg tgcagcgata gaccttgcct tcgtcgggat cgaggatctc
     2761 gccgccggtg taagcgtcgg cctcgggccg catcccccac aggatcttca tgcccacgat
     2821 cggctggttg cgcagctcgc cagggcattc ctcgcaaagc ggatttgcgc tctgcgcggg
     2881 cggcgaaaac accttcacca ccgttccctc gagctcgccg ttcgcctgcg tgacgcgcac
     2941 gagcccgtcg gcctcgccgg tgcgatcgct ataggtcttc catagcccca cgggcgtcgg
     3001 cgcctgcgcg gcggcagcgc cgtagacgca gaggagcacg aggaacgacg cggctctcat
     3061 ggcgcggcta gcctatcatt gccgtgatga gctctcgaag cctgcgtggc gacttgctga
     3121 ggctgctgct gctgccgatc tccgcaatcc tggtggtgag cggcgtgtcg gcttattacc
     3181 tcgcgctcga gcccgccacc gaggcgctcg atgcgagcct catcgacgcg ggaatcgcga
     3241 tccgcgagcg cattttcgtg cgcgacgggc tcgtgacggt ggacctgccg acggcagcag
     3301 agcaggtcct gcgcgcggac cgctacgatg cggtttacta cgtggtgcgc gatccgggcg
     3361 gcaacctgat cgcgggcgac tccggcatcc cgctgccgcc gcccggacag caggcccagg
     3421 acaacgtcat gttctacgac gccgagttcc gcggcgcgcg cgtgcgcgga gcaacgctgc
     3481 ttgcgccctg cggcaaggga ccttgcgcgg tgaccgtcgc cgaaaccacg cgcaagaggg
     3541 accgcc
//
LOCUS       DATDMP010000532         3199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_753452, whole genome shotgun sequence.
ACCESSION   DATDMP010000532 DATDMP010000000
VERSION     DATDMP010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753452"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VI195_09320"
     CDS             <1..205
                     /locus_tag="VI195_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6824629.1"
                     /translation="HCPQRDFGFIARQRGMFSFFGIDTAQVHALRERHHVYMTDDSRM
                     NIAGLRAENLEYFAHAVAEVLAR"
     gene            complement(226..735)
                     /locus_tag="VI195_09325"
     CDS             complement(226..735)
                     /locus_tag="VI195_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004406045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY6824630.1"
                     /translation="MRASKTPAAGDSTAQRVLDRIDRRLLTRLQQDGRAPVSQLAREV
                     HLTVTPTLERVRRLESGGYIEGYFARLSPRRLGLGLLAYVEVLLDRTTPDAFERFKQA
                     MLAHDEVMECHMVAGGFDYLLKVRVTDMESYRRFLGDRIATVRGVQQTHTYFVMEEVK
                     STHRVIVPE"
     gene            837..>3199
                     /gene="putA"
                     /locus_tag="VI195_09330"
     CDS             837..>3199
                     /gene="putA"
                     /locus_tag="VI195_09330"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY6824631.1"
                     /translation="MASELMEKIGGTGTLAELRTRLRAATLRPEAAASDETLADLTPA
                     QEALERARVRAARWVQVARADQRSRPLVERMLEQFPLDSAQGKALMSLAEALLRTPDP
                     KRADQLIAERLATIREAGVPGDTDLLLRTGFTLLGAAGRLLPDVLGELAGEFSTASLT
                     RPVVAPMVRAALRQSMQLLGHAFIVGETIDAARARGADDAELALCSYDVLGEGARTDA
                     DAQRYYEAYAHAIEALQRQAGQGTHARSGISVKLSALEPRYTLLQSTRVHERLAPRML
                     ELARSAATAGIGFTIDAEEADRLDLSLDILETLARDARTRDWDGLGLAVQAYGRRAPL
                     VIAWVARLARETGRRMSVRLVKGAYWDSEIKRAQERGLASFPVYTSKAATDASYLECV
                     QHLFAARELIYPQFATHNAYTVAAVLELAPPAAAYEFQRLHGMGEALYAAARAEVPDL
                     PPVRVYAPVGTHEELLPYLVRRLLENGANTSFVHQFMNPQIPVEEVVRDPVASLAAEP
                     ARPARLREPSALFGAERANSAGEDFGDPATLAALASELRALAEVVHSGGPILSGRLAR
                     ELKVPVSSPADHGEVVGHTRDATAGEIRTAMQEAAAAQPAWDATPAEARAACLLEAAD
                     LLQQRRGIFLSLLVREAGKTLPDALAELREAVDFCRYYAARGRELFGAPEELPGPTGE
                     RNTLALNGRGVFVCISPWNFPLAIFTGQISAALMAGNAVVAKPAPATPLIAHAMTRLL
                     HEAGVPPAVLQLTPADGPPFGEVALTHPALAGVAFTGSTATASTINRT"
BASE COUNT          493 a         1117 c         1081 g          508 t
ORIGIN      
        1 gcattgccca cagcgggact ttggcttcat cgcgcgccag cgcggcatgt tctcattctt
       61 cggcatcgac accgctcagg tacacgcgct gcgcgagcgc catcacgttt acatgaccga
      121 tgacagccgc atgaacattg ccggcctgcg cgccgagaac ctggagtatt tcgcgcatgc
      181 cgtggcggag gtgctcgcgc gctagctgtc ggcccggatg ccccatcact ccggcacgat
      241 cacgcggtgg gttgacttca cctcttccat gacgaagtag gtgtgcgtct gctgcacgcc
      301 gcgcaccgtg gcgatgcggt ccccgaggaa acgccggtag ctctccatgt cggtgacgcg
      361 caccttgagc agatagtcga agccacctgc caccatgtgg cactccatca cctcatcgtg
      421 cgcgagcatc gcctgcttga agcgctcgaa tgcatccggc gtggtccggt cgagcaagac
      481 ctcgacgtag gcgagcagcc cgagtccgag cctgcgcggg ctcagccgcg cgaagtagcc
      541 ctcgatgtag ccgcccgact cgaggcgccg cacccgctcg agcgtcgggg tgacagtgag
      601 gtgcacctcg cgcgcaagct gcgagaccgg agcccgacca tcctgctgca gcctcgtgag
      661 gagccgccgg tcgatgcggt cgagcacgcg ctgcgccgtc gagtcacctg ccgcaggggt
      721 tttcgatgcc ctcatggtag ttaattttac tattagggag ctctaaatgg ataattattc
      781 tctagagctg gcgctagaat aagcagaatt aactatcaag agacgatggg cacctcatgg
      841 cgagtgagct gatggagaaa attggcggaa cgggcacgct cgcggagctg cgtacgcggc
      901 tgcgcgccgc gacgctgcgt ccggaggccg ctgcatccga tgagacgctc gccgatctca
      961 ccccggcgca ggaagccctc gagcgggcgc gggttcgcgc cgcccgctgg gtgcaagtag
     1021 cgcgcgccga ccagcgctcg cgtccgctcg tcgagcggat gctcgagcag ttcccgctcg
     1081 acagcgctca gggcaaggcc ctcatgagcc tcgcggaagc actgttgcgt acgcccgatc
     1141 ccaagcgcgc cgatcagctg atcgccgagc gcctcgccac catccgcgag gcgggcgtgc
     1201 ccggggatac cgatctgctg ctgcgtacgg gtttcaccct gctcggggcc gccggacgac
     1261 tgctgcccga tgtactgggg gagctcgccg gtgagttctc caccgcgagt ctcacgcgtc
     1321 cggtggtcgc accgatggtg cgggcggcgt tgcgtcagtc gatgcagctc ctcggccacg
     1381 ccttcatcgt gggcgagacc atcgatgcgg cacgcgcgcg cggcgcggac gatgcggagc
     1441 tcgcactgtg ctcttatgac gtgctcggag aaggggcgcg caccgacgcc gacgcgcagc
     1501 gctattacga ggcctacgca cacgccatcg aggcactgca gcgtcaggct ggccagggta
     1561 cgcacgcgcg ctcgggaatc tcggtgaagc tctcggcact cgagccgcgc tacacgctgc
     1621 tgcagagcac gcgcgttcac gagcggctgg caccgcgcat gctggagctg gcgcgcagcg
     1681 cagctaccgc gggcatcggc ttcaccatcg acgccgaaga ggccgatcgg ctggatctct
     1741 cgctcgacat cctcgagacg ctcgcccggg atgcgcgcac gcgcgactgg gacgggctcg
     1801 ggctcgccgt gcaggcctac gggcggcgcg ccccgctcgt gatcgcctgg gtggcgcggc
     1861 tggcacgcga aaccggccgg cgtatgagcg tgcggctcgt gaaaggcgcc tactgggaca
     1921 gcgagatcaa gcgcgcacag gagcggggtc tcgcgagctt tcccgtctac acctccaagg
     1981 ccgccaccga cgccagctat ctcgagtgtg tgcagcatct gttcgcggcg cgcgagctga
     2041 tctatccgca gttcgcgacc cacaatgcct acaccgtggc cgcggtgctc gagctggcgc
     2101 cgccggctgc cgcgtatgaa ttccagcgcc tgcacggtat gggagaggcc ttgtacgcgg
     2161 cggcgcgtgc cgaggtcccg gatctgccac cggtgcgcgt gtatgcgccg gtcggcactc
     2221 acgaggagct gctgccgtac ctcgtgcggc ggctgctcga gaacggcgcc aacacttcct
     2281 tcgtccatca gttcatgaat ccgcagatac ccgtggagga ggtcgtgcgc gacccggtcg
     2341 catcgctcgc tgccgagccc gcccggccgg cgcgcctgcg tgagccgagc gcgctgttcg
     2401 gcgccgagcg cgccaactcc gcgggcgagg acttcggtga tcccgccacg ctcgccgcgc
     2461 tcgcgtccga gctgcgcgcg ctggcggagg tcgtccattc cggtgggccg atactgagcg
     2521 gtcgactggc gcgcgagctg aaggtgccgg tgagctctcc agccgaccac ggcgaggtcg
     2581 tgggacacac ccgcgatgcc actgcagggg agatccgcac cgccatgcaa gaggccgccg
     2641 cggcgcagcc cgcctgggat gcgacgccgg ccgaggcgcg cgcggcctgc ctgctcgagg
     2701 ccgccgacct gctgcaacag cggcgcggca ttttcctcag cctgttggta cgcgaggccg
     2761 gcaagacgct gccggatgcg ctggcagagc tgcgcgaagc ggtcgacttc tgccgctact
     2821 acgccgcgcg cggtcgcgag ctcttcggtg ctcccgagga gttgcccggt ccgacgggcg
     2881 agcgcaacac gctcgcgctc aacggccgcg gcgtgttcgt gtgcatcagc ccgtggaact
     2941 ttccgctggc gattttcacc gggcagatca gcgctgcgct catggcgggg aatgccgtgg
     3001 tcgcgaagcc ggcgcccgcc accccgctca tcgcgcacgc catgacccgg ctgctgcacg
     3061 aagcgggtgt cccgccggca gtgttgcagc tcacccccgc ggacggcccg cccttcggcg
     3121 aggtcgcgct cacgcatcca gcactcgcag gcgtcgcctt taccggctcc accgccaccg
     3181 ccagcaccat caaccgcac
//
LOCUS       DATDMP010000533         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_530690, whole genome shotgun sequence.
ACCESSION   DATDMP010000533 DATDMP010000000
VERSION     DATDMP010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530690"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VI195_09335"
     CDS             <1..335
                     /locus_tag="VI195_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824632.1"
                     /translation="RRAVDSFDADLNVQVWGTGGMSHQLQGPRAGLINEPWDQRFIER
                     LINEPQALAQLPHLEYVREAGSEGIELVMWLIMRGALNEKVRTVHRFYHVPASNTAVG
                     HLILENLP"
     gene            332..1282
                     /locus_tag="VI195_09340"
     CDS             332..1282
                     /locus_tag="VI195_09340"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6824633.1"
                     /translation="MKIVLAGAGAFGAKHLDAIKAIGGVEVVSLVGRTLEPTRALAMK
                     YGIEHVTTDLAEALGRPGVEAAILVTPTPLHASQAIACLEAGKHVQVEIPLADNWADA
                     QAVANLQKRSGLVCMVGHTRRFNPSHQWIHKRIAQGELSIQQMDVQTYFFRRTNINAL
                     GAPRSWTDHLLWHHAAHTVDLFTFQTGSPVSIAHALQGPIHPTLRIAMDMSIQLKAAS
                     GAICTLSLSFNNDGPLGTFFRYICDHGTYIARYDDLIDGKEHKIDVSHVDVSMNGIEL
                     QDREFFAAIREGREPNASVAQVLACYRTLADLERQLNAQG"
     gene            1327..1866
                     /locus_tag="VI195_09345"
     CDS             1327..1866
                     /locus_tag="VI195_09345"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6824634.1"
                     /translation="MGQVGFGQPDDGVIQMAYVVADLRAAIAQWSRTLRIGPWFVLEH
                     FSGVDPVYRGQPSRADVAIAMSFAGHMNIELIQVNNDAPSVYRELIERRGYGFHHWGV
                     ATWNFDAAVAQYEREGYPLAFGAAVPSGGRVGYMDTTSVLPGFTEFIELGGAFEEVFG
                     RFYRASIDWDGKDPIRSFI"
     gene            1868..>2185
                     /locus_tag="VI195_09350"
     CDS             1868..>2185
                     /locus_tag="VI195_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824635.1"
                     /translation="MRYEQRLIWVLGITFGFLFFDRNAASFLMPFIARDLHFNNQQVG
                     LIASALSFTWAVSALLGGALSDRTGSRKPVLLASAVAFSLCSFLSGLAGSFVALFTAR
                     LLMG"
BASE COUNT          372 a          713 c          683 g          417 t
ORIGIN      
        1 tgcggcgcgc ggtcgattcc ttcgatgcag atctcaacgt acaggtctgg gggaccggcg
       61 gtatgagcca ccagctgcag ggaccacgcg ccggcctcat caatgagccc tgggaccagc
      121 gcttcatcga gcggctgatc aacgagccgc aagcgctcgc gcagctgccg cacctcgaat
      181 acgtacgcga ggcgggatcg gagggcatcg agctcgtgat gtggctcatc atgcgcggcg
      241 cgctcaacga gaaggtccgc accgtgcacc gcttctatca cgtgccggct tcgaacacgg
      301 cggtgggcca cctcatcctg gagaatctgc catgaaaatc gtgctcgccg gagcgggcgc
      361 tttcggcgcg aaacatctcg atgcgatcaa ggcgatcggc ggcgtcgaag tggtgtcgct
      421 cgtggggcgt accctcgagc cgacgcgtgc gctcgcgatg aagtatggca tcgagcacgt
      481 caccaccgat ctcgccgagg cgctcggccg tcccggtgtc gaagccgcga tactcgtgac
      541 cccgacaccg ttgcacgctt cccaggccat cgcctgcctc gaggccggca agcacgtgca
      601 ggtcgagata ccgctcgccg acaactgggc ggacgcccag gcggtcgcga atctgcagaa
      661 gcgcagcgga ctcgtctgca tggtcgggca cacgcgccgc ttcaatcctt cccaccagtg
      721 gattcacaag cgaattgcgc aaggtgagct ctcgatccag cagatggatg tgcagacgta
      781 ttttttccgg cgcaccaaca tcaacgctct cggtgcaccg cgttcctgga ccgatcatct
      841 cctgtggcat cacgccgccc acacggtcga tctgttcacc tttcagaccg gctcgccggt
      901 gagcatcgcg cacgcgctgc agggcccgat tcacccgacc ttgagaattg ccatggacat
      961 gtccattcag ctcaaggccg cgagcggtgc gatctgtacg ctgtccttgt ctttcaacaa
     1021 tgacggcccg ctcggcacct tcttccgcta catctgcgac cacggcacgt acatcgcgcg
     1081 ctacgacgac ctgatcgatg gcaaggagca caagatcgat gtgtcccatg tggatgtgtc
     1141 gatgaatggc atcgagctgc aggatcggga gttcttcgca gcgatccgcg aggggcgcga
     1201 accgaatgcg agtgtcgccc aggtactggc gtgctaccgc acgctcgccg acctcgagcg
     1261 ccagctcaac gcgcagggct aacggcaact acggcacgcg gataaatttc cgcccgggga
     1321 ggcgtcatgg gtcaggtagg tttcggacag ccggacgatg gcgtcatcca gatggcctat
     1381 gtcgtggcgg acttgcgcgc ggcgatcgcg caatggtcga ggacgctgcg catcggcccc
     1441 tggttcgtgc tcgagcattt cagcggcgta gacccggtgt atcgcggaca gccctcgcgc
     1501 gcggacgtgg cgatcgccat gagctttgcc gggcacatga acatcgagct catccaggta
     1561 aacaacgacg cgccctccgt ctaccgcgag ctcatcgagc ggcgcggcta cggcttccac
     1621 cactggggcg tggcgacctg gaacttcgat gcggcggtcg cgcagtacga gcgcgagggt
     1681 tacccactcg cctttggcgc tgcggtaccg agtggcggcc gtgtggggta catggatacc
     1741 acttcagtcc tgccggggtt caccgaattc atcgagctcg gcggcgcttt cgaagaggtg
     1801 ttcgggcgct tctaccgcgc gagcatcgac tgggacggca aggatccgat tcgctccttc
     1861 atctgacatg cgctacgaac aacgtctgat ctgggtcctc ggcatcactt tcggctttct
     1921 gtttttcgac cgcaatgcgg ccagcttcct gatgccgttc atcgcgcggg atctgcactt
     1981 caacaatcaa caggtcggcc tcatcgcctc ggccctgtcc ttcacctggg cggtctcggc
     2041 attgctcggc ggcgcgctgt cggaccgcac cggcagccgc aagccggtgc tgctcgcgag
     2101 cgccgtggcc ttctcgttgt gctcgttcct ctcggggctc gccgggtcgt tcgttgccct
     2161 gttcactgcg cggcttctca tgggc
//
LOCUS       DATDMP010000534         3658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1863910, whole genome shotgun sequence.
ACCESSION   DATDMP010000534 DATDMP010000000
VERSION     DATDMP010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863910"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..86)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            336..>3658
                     /locus_tag="VI195_09355"
     CDS             336..>3658
                     /locus_tag="VI195_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824636.1"
                     /translation="MQIAERRRRLAPPLLVLVLTLLVAVRAAASAELSVPPELRGWED
                     WALQGHESHRCPWLVPGKPTDDLRICAWPSVLELQVDEHGGRFTQRWEAASDTWLPLP
                     GGTDNWPEEVSLDASPAAVVVHDGTPGVRVAAGAHSMTGVFRWMRRPELLSLPRSVAL
                     VSLTIDGARVLNPQRNDAGIVLGAHATAREEERADVRVFRRLDDRIPALLRTEIHLAV
                     AGEARELRLPAALPPGFQPTAIESPLAARLDPDNTLRVQVRPGDFGVTIEARGPSPVT
                     EVRLPQRSTPWPREEVWSFAADDRLRVVAIEGVAAVDPAQADVPQQWRELPAYRMDSG
                     TVLKVIERSRGLSAADGNDLRLERTAWLGFAGKGYTIVDKLSGMMRQDWRLDLEAPYA
                     LRSARTGSGEPLLVTSGVAHGLSGIELRTPSLDLTAVSQLERVRGLLPATGWHTRFTS
                     VSGKLVLAPGYRLLAAVGPDAAPQAWLERWRLLDIFIVLLTATVAWRTFGVRTALIAL
                     AAAALTYQESGAPTWLWLAVLIAIALHRAAPAGRLRDCAGGARLLALALLLLSLIPFG
                     ITQVRLAVYPQLEALGLGAYTGYALAAAPAAPAVRHETLPERAVVEGIAYEAKTASAL
                     SSMAVSQDVAVTAARRTAAAEQFARYEPGALVQAGPGVPDWSYHVYSFSWSGPVEESA
                     TARFLISPPWTTRLWRLVGLLLAALFVIELTRRELPRLPSSWRARPQAAVGVVLVAVA
                     LASGAVPTADGATTPDPGLIDELRDQLLAPPKCAPDCAALLSADVTATAARLSVVLTV
                     SALDAVGVALPGAEPNWTPDLVQVDGAGGAWVLRSPDGVRHVNLPRGRHVVRIEGPLE
                     GSEALSLSFPLRPQVIDAHAPDWDLGGVSERHLMSGALELARKRVASTAPGGATARQE
                     EFPPFVVVDRLFHLSHEWTIDTTVTRVAPKTAAFTVNLPLLPEEAVTTPGLKSSGGRL
                     SLGLAAAERLQQFSSILPRSDSLELVASRDHSNTERWRFEVAPTWHADFSGIPAVAPD
                     QDGAIWLFEYYPRPGERLAVRVTRPTAAAGGTLAFDSVALETSVGRRSSDTSLELHYR
                     STQGGRQTLRIPANAEVTKV"
BASE COUNT          513 a         1249 c         1285 g          611 t
ORIGIN      
        1 caggagttgg cacctttcca gagtggttgg ttctggcggt tgccccggcg tctaagggcc
       61 tatccctcag ccggtctcga tgagtgaggg ggagtttacg gggcgggctg ggggcggtgc
      121 aagccgcaga tgttcccccg ggttttcccc acgggaggtt tcgggtaata gagtgcgcaa
      181 cgcgccaggc gggcggcggc gcgcggaggc atgacatacc gagcggccgc cggggcgcca
      241 ggcagcgagc caacaccgcg ccgcaggcgg tgaacccttt gcagaagcca acatctaaca
      301 ggcacgcggc acaattagcc gcggggggca cgtccatgca gatcgctgag cgccgtcggc
      361 ggctcgctcc tcctcttttg gttctcgtac tcaccctgct ggtagcggtg cgcgccgcgg
      421 cgtccgcgga gctctccgtg ccgccggagc tgcgcggctg ggaggattgg gcgctgcagg
      481 gtcacgagtc gcatcgctgc ccgtggctgg tgccgggtaa gccgaccgac gacctgcgca
      541 tctgcgcctg gccctcggtg ctcgagctgc aagttgatga gcatggcggg cgcttcactc
      601 agcgctggga ggcggcgagc gacacctggc tgccgctgcc cggcggcacg gacaactggc
      661 ccgaagaggt gagcctcgat gccagccctg ccgccgtggt agttcacgac gggacacccg
      721 gcgtgcgggt ggccgcaggc gcgcattcga tgaccggagt gtttcgctgg atgcggcggc
      781 ccgagctgtt gagtctgccg cgatcggtgg cgctcgtgag ccttacgatc gatggtgcac
      841 gagtcctcaa tccgcaacgc aacgatgcag gaatcgtcct cggtgcacac gccaccgccc
      901 gggaggagga gcgtgccgac gtgcgcgtgt tccgccgcct cgacgaccgc attccggcgc
      961 tgctgcgcac cgaaatccac ctcgcggtcg ccggcgaggc acgcgagcta cgcctgccgg
     1021 cggcgttgcc gccggggttt cagccgacgg cgatcgagtc gccgctcgct gcacggctcg
     1081 accccgacaa cacactgcgc gtacaggtgc ggccgggcga tttcggcgtg acgatcgagg
     1141 cgcgggggcc gagccccgtt acggaagtgc gcctgccgca gcgctccaca ccgtggccca
     1201 gggaggaggt atggagcttc gcggccgacg atcgattgcg cgtcgtggcc atcgagggtg
     1261 tcgcagcggt ggatccggcc caggcggacg tgccgcagca gtggcgtgag ctgcccgcct
     1321 atcgcatgga ttccggcacg gtcctcaagg tcatcgagcg cagccgcggc ctttccgccg
     1381 ccgacggcaa cgatcttcgg ctggagcgca ccgcctggct cggcttcgca ggcaagggtt
     1441 acaccatcgt cgacaagttg agcggcatga tgcgccagga ttggcgtctc gacctcgagg
     1501 cgccctacgc cctgcgcagc gcgcgtaccg gctcgggcga gccgctgctc gtcacctccg
     1561 gcgtcgcaca cgggctgagc ggcatcgagc tgcggacacc ctcgctcgac cttactgcgg
     1621 tatcgcagct cgagcgcgtg cgcggattac ttcctgcgac cggctggcat acccgcttca
     1681 cgagtgtctc cggcaagctg gtgctggcgc ccggctatag gctgcttgcc gcagtcggac
     1741 ctgatgcggc gccgcaggcg tggctcgagc gctggcggct gctcgatatt ttcatcgtgt
     1801 tgctcaccgc aaccgtcgcc tggcgcacgt tcggcgtacg cacggcgctc atcgcgctcg
     1861 cagccgccgc gctcacctat caggaaagtg gtgctcccac ctggctatgg ctcgccgtgc
     1921 tgattgcgat tgccctgcac cgtgccgctc ctgccggcag actgcgcgat tgcgccggag
     1981 gcgctcgcct gctggcgctc gcgttgctgc tgctctcgct cattcctttc ggtatcaccc
     2041 aggtacgtct tgccgtgtat ccccagctcg aggcgctcgg cctcggtgcc tacacgggct
     2101 acgcgctcgc ggccgctcct gccgcgccgg cggtgcggca cgaaacgctg ccggagcggg
     2161 cggtggtcga gggaatcgcc tacgaggcaa agaccgcctc ggccctctcg agcatggccg
     2221 tcagccagga cgtcgcggtc actgccgcgc gcaggacggc cgcggcggag cagttcgctc
     2281 gctatgagcc gggcgctctc gtgcaggcag gcccgggcgt gccggactgg agctaccacg
     2341 tctactcctt ctcctggagc ggaccggtcg aggagagtgc caccgcgcgc tttctcatca
     2401 gtccgccttg gacgacgcgg ctgtggcggc tggtgggtct cctgctcgct gcgctctttg
     2461 tgatcgagct gacgaggcgc gagctgccgc gactaccctc ctcctggcgc gcacggccac
     2521 aggcggcggt cggcgtcgtg ctcgttgcag tggcactcgc aagcggtgca gttccgaccg
     2581 ccgatggagc gaccacgccc gacccgggcc tcatcgatga gctgcgcgac cagctgcttg
     2641 cgcccccgaa gtgcgcaccc gattgtgccg cactgctgtc cgcggacgtc actgctacgg
     2701 cggcgcggct cagtgtggtg ctgaccgtca gtgccctcga tgccgtcggc gtggcactgc
     2761 ctggggccga gcccaactgg acgccggacc tggtgcaagt tgatggtgcg gggggcgcgt
     2821 gggtgctgcg cagtccggac ggtgttcggc acgtgaatct tccgcgcggg cgacatgtgg
     2881 tgcgcatcga ggggcccctg gagggcagcg aggctctgag cctgtcgttt ccgctgcgtc
     2941 cgcaggtgat cgacgcgcac gcccccgact gggatctggg tggagtcagc gagcgtcacc
     3001 tgatgagcgg ggctctcgaa ctcgcgcgca aacgtgtggc gagcacggct cctggaggcg
     3061 ccactgcgcg gcaggaggag tttccaccct tcgtggtcgt cgaccgtctt tttcaccttt
     3121 cccacgagtg gacgatcgac accaccgtca cgcgtgtcgc gccgaaaacg gcggccttca
     3181 cggtcaacct gccgctgcta ccggaggaag cggtcacgac accgggcctg aagagcagcg
     3241 gcggccggct ctcgctgggg ctcgctgccg cagagcgcct gcagcagttc agctcgatcc
     3301 tgccgcgaag cgattcactc gagctggtgg cctcgcgcga ccattcgaac actgagcgct
     3361 ggcgcttcga agtcgcacca acctggcacg ccgatttcag cggtatcccg gccgtcgcac
     3421 ccgatcagga cggagcgatc tggctgttcg agtactaccc gcgtccggga gagcgcctcg
     3481 ctgtgcgggt tacacggccc accgccgcgg cgggcggcac gctcgccttc gacagcgtcg
     3541 cgctcgagac cagcgtcggc cggcgctcga gcgacacgag tctggagctg cattaccgca
     3601 gcacccaggg cggccggcag accttgcgca taccggcgaa cgcggaggtc accaaggt
//
LOCUS       DATDMP010000535         4150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_420492, whole genome shotgun sequence.
ACCESSION   DATDMP010000535 DATDMP010000000
VERSION     DATDMP010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420492"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..862)
                     /locus_tag="VI195_09360"
     CDS             complement(95..862)
                     /locus_tag="VI195_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824637.1"
                     /translation="MTLADAAEWLHAWARAGRFALVAAAAGLSRSTYTTEMRTVLLRQ
                     MYFTAWEVAVGFTLFASFLSLVVVEITINAARVYGLASYALELVFRVLVLEILPFGTA
                     LFIAIRSGSALSTEVALMHSTGELDRLHAAGVDPFEREFVPRVVAAALSVLALTVLSC
                     GMAMVVAYLGMYGFSPWGFGEYTHTVARVFSEPTLAGLALKCIVFGVVVAVIPIAAGL
                     DATSNLKSAPLAVMGGMVRLVLALALLEVLSLAVKYV"
     gene            877..1917
                     /locus_tag="VI195_09365"
     CDS             877..1917
                     /locus_tag="VI195_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY6824638.1"
                     /translation="MGEKRIAHEKLTAFAAACLEKLGLLQADARLVAETLVAANLRGV
                     DSHGVVRLAHYATRLRNGTVKARPSITVQRTGASSGVVEGDAGMGQLVAERAMREAIA
                     LAKESGVGAVVARNSSHCGACAYFVELAVREGLVGIALTHTDSIMVPPGMQRIFLGSN
                     PIAFGAPAGDEPPLIIDMSTTHAAWGKIIVARQEGKPIPPDWALDAGGKPTTDASLAV
                     GLAPTGGHKGFALALMIEILCAQLAGVPFGPHVTKMYGELEKPRNLGHFMLALDVARY
                     TGGAAFREQIDLLVREIRSQEPSDPARPPLAPGDPERLCAAARTKDGVPLGEGVLAEL
                     NALAASLGVAQI"
     gene            complement(1914..2984)
                     /locus_tag="VI195_09370"
     CDS             complement(1914..2984)
                     /locus_tag="VI195_09370"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEY6824639.1"
                     /translation="MKNFQVAPLTRAIEAIALAGGSNAEEARMVAENLVMANLCGHDS
                     HGIGMMPRYVEALQEGGLAVNQKPKVQLDAAALLVLDGCKGYGQTIGFAATNLAIARA
                     KQHGCAIVALGNSHHLGRIGHWAEMAVREGLISMHFVNVQSFARVAPFAGADRRFGTN
                     PVCIGIPLPGEAPFLLDMATSAVAQGKLRVAHNKGAKIPHGWLIDDQGNPTEDPRWGV
                     VAPLGAMLTFGEHKGYGLAVACELLGGALTGGGVTDYDNKTRRRVLNGMLSILIDPKR
                     LGTADSFAKDAKSFLAWVRASRAAPGADRVRIAGEPEREMRARRERDGIPVDAETWKE
                     IGAAAKKLGLAPDKVERLARGG"
     gene            3159..>4150
                     /locus_tag="VI195_09375"
     CDS             3159..>4150
                     /locus_tag="VI195_09375"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY6824640.1"
                     /translation="MNKTKKPEELRSFRWYGAQDLRAFGHRSRTAQMGYERSDYAGKP
                     VIAIINTWSDINSCHTHFKQRVEEVKRGVWQAGGFPVEMPAMSLAEVMQKPTTMMYRN
                     FLAMETEELLRSYPADGAVLMGGCDKTTPALVMGATSMNLPTIYMPAGPMLTGHWREN
                     TLGSGSDTWKYWAELRAGNITERDWQEIEDGIARSPGTCMTMGTAATMMSITEALGLA
                     LPGASSIPAPDSNHAKMATHCGKRIVEMVWEDLKPRDILSKESFDNAIVTLMAMGGST
                     NALIHLIAMAGRAGVKLPMERYNEFSARTPLLANVRPSGDKYLMEDFYYAGGLRA"
BASE COUNT          749 a         1392 c         1366 g          643 t
ORIGIN      
        1 cggaattgga ggttcttcgg tatcggggtg aagcgcggct ccatcggaca tactttaccg
       61 cgtctgtgag ctagacctcc ttagcgccgc ctcactaaac gtacttcacg gccagcgaaa
      121 gcacttcgag aagcgcgagg gcaagaacga ggcgcaccat accgcccatc accgccagcg
      181 gcgccgactt caggttgctc gtcgcgtcca gccccgcggc aatggggatc actgccacca
      241 ccacgccgaa cacgatgcac ttcagggcaa gcccggcgag cgtaggctcg ctgaaaacac
      301 gggccacggt atgcgtgtac tcgccaaatc cccagggcga gaatccatac atacccaagt
      361 aagcaacgac catggccatc ccgcagctga ggaccgtgag cgccaaaacc gagagcgccg
      421 ccgccacgac gcgcggcacg aactcgcgct cgaagggatc cacgccggcg gcgtgcagcc
      481 tgtccagctc gcccgtcgaa tgcatgagcg cgacttccgt gctgagcgcg gagccggagc
      541 ggatcgcaat gaaaagcgcg gtgccgaagg gcaggatttc aagcacgagc acgcgaaata
      601 ccagctcgag cgcataggac gcgagcccgt acactcgcgc cgcgttgatg gtgatctcca
      661 ccaccacgag gctcagaaac gacgcaaaca gcgtaaatcc cacggcaact tcccaggcgg
      721 tgaagtacat ctggcgcagg aggacggtcc gcatctcggt cgtgtaggtg gaccgggaca
      781 aacccgcggc cgccgcgacc aatgcaaagc ggcccgcgcg ggcccaggca tggagccact
      841 cggcggcatc ggcgagcgtc atctaaaatc ccgcccatgg gagaaaaacg gatcgcacac
      901 gaaaagctta ccgcgttcgc cgcggcgtgc ctggaaaagc tgggtctttt gcaggccgat
      961 gcgcgactcg tggccgaaac gctggtggcg gcgaacctcc gcggcgtaga cagccacggc
     1021 gtcgtgcggc tcgcgcatta cgcgacgcgc ctgcgcaacg gcacggtgaa ggcccggcca
     1081 agcataaccg tccagcgcac cggagcctcg agcggagtcg tcgaaggcga cgcagggatg
     1141 gggcagctcg tcgccgagcg cgccatgcgc gaagccatcg cgctcgccaa ggagagcggc
     1201 gtgggcgcag tggtggcgcg caacagctcg cattgcggcg catgcgcgta cttcgtggag
     1261 ctcgcggtgc gcgaaggcct ggtgggcatc gcgctcaccc atacggattc catcatggtc
     1321 ccgcccggga tgcagcgcat cttcctcggc tcgaatccca tcgcgttcgg cgcgccggcg
     1381 ggcgacgagc cgccgctcat catcgacatg tcgaccacgc acgcggcctg gggcaagatc
     1441 atcgtcgcgc gccaggaagg caagccgatc ccgccggatt gggcgctcga tgcgggcggc
     1501 aagcccacca ccgatgcgag cctggccgta ggccttgccc cgaccggggg ccacaaaggc
     1561 tttgcgcttg cgctgatgat cgagatcctt tgcgcgcagc tcgccggcgt gccgttcggc
     1621 cctcacgtga cgaagatgta cggcgagctc gagaagccgc gcaatctcgg gcatttcatg
     1681 ctggcgctcg acgtcgcgcg ctataccggg ggcgcggcgt ttcgtgagca gatcgatctg
     1741 ctggtgcgcg aaatccgctc gcaggaacct tcggaccccg ctcgcccgcc gctcgcgccg
     1801 ggcgacccgg agcggctttg cgcggcggcg cgaacgaagg acggggttcc cctcggcgaa
     1861 ggggttttgg ccgagttgaa tgctcttgcc gcgagcttag gggtggcgca gatctaacct
     1921 ccgcgcgcga gcctttcgac tttgtccggc gcaagaccga gcttcttcgc ggctgcgccg
     1981 atctccttcc acgtttccgc gtccaccgga attccgtcgc gctcgcggcg agcgcgcatt
     2041 tcgcgctccg gttcgccggc gatgcgcacg cggtccgcgc ccggcgccgc ccgcgatgcg
     2101 cgcacccagg cgaggaaact cttggcatcc ttcgcgaacg aatccgcggt gcccaggcgc
     2161 ttcggatcga tgaggatcga gagcatgccg ttcagcacgc ggcggcgcgt cttgttgtcg
     2221 tagtcggtta cgccgccacc ggtgagcgcg ccgccaagaa gctcgcaggc cacggcaagg
     2281 ccgtagccct tgtgctcgcc gaaggtcagc atcgccccga gcggcgcgac gaccccccag
     2341 cgcggatcct cggtcggatt gccctggtcg tcgatgagcc agccgtgcgg aatcttcgcg
     2401 cccttgttgt gcgccacgcg cagctttccc tgcgccaccg cgctcgtcgc catgtcgagg
     2461 aggaacggcg cctcgcccgg aagcggaatg ccgatgcaga cgggattcgt gccgaagcgc
     2521 cggtcggcgc cggcgaacgg cgcaacgcgc gcgaaggact gcacattcac gaagtgcatc
     2581 gagatgagcc cctcgcgaac cgccatctcc gcccagtggc caatgcggcc gaggtgatgc
     2641 gaattgccga gcgccacgat cgcgcacccg tgctgcttcg cgcgcgcgat ggcgaggttg
     2701 gtggcggcga accctatggt ctgcccatag cccttgcagc cgtcgagaac caggagcgcc
     2761 gcggcatcga gctgcacctt cggcttctgg ttcacggcga ggccgccctc ctgcagcgcc
     2821 tcgacgtagc gcggcatcat gccgatgccg tgcgagtcgt ggccgcacag attcgccatg
     2881 acgaggttct cggccaccat tcgcgcttcc tcggcgttgc tgccgccggc gagcgcgatc
     2941 gcttcgatgg cccgggtcag aggtgctacc tggaaattct tcattgccgt gtctctcgcg
     3001 aagccgccga gtttacttcg caaccgtggt atatagcgag cgatgagact tctcgtgctg
     3061 gtcgcggtgc tcgctgcagc gtcggcccat gcgcagacct gggctgcgcg cggagcaggc
     3121 gcgctggggg aaagtcgtca agaagtccgg agtcgaattt gaataagacc aagaagccgg
     3181 aagagctgcg cagcttccgc tggtacggcg cgcaggacct gcgcgccttc gggcatcgct
     3241 cgcgcaccgc gcagatgggc tatgagcgca gcgattacgc cggcaagccg gtcatcgcga
     3301 tcatcaacac ctggtcggac atcaattcct gccatacgca cttcaagcag cgcgtcgagg
     3361 aggtgaagcg cggcgtgtgg caggcgggcg gcttcccggt ggaaatgccg gcgatgtcgc
     3421 tcgcggaggt gatgcagaag cccaccacca tgatgtaccg caatttcctc gccatggaga
     3481 ccgaggagct gctgcgttcc tatcccgctg acggcgccgt gctgatgggc ggctgcgaca
     3541 agaccactcc ggcgctcgtg atgggcgcga cgagcatgaa cctgccgacg atctacatgc
     3601 cagcgggccc gatgctcacc ggccactggc gcgagaacac gctcggctcc ggatcggata
     3661 cctggaaata ctgggccgag ctgcgcgcag gaaacatcac ggagcgcgac tggcaggaga
     3721 tcgaggacgg catcgcgcgc tcgccgggca cgtgcatgac gatgggcacg gctgcgacca
     3781 tgatgtccat caccgaagcc ctcgggctgg cgctgcccgg cgcctcctcg atcccggcac
     3841 cggactccaa ccacgccaag atggccaccc attgcggcaa gcgcatcgtg gagatggtgt
     3901 gggaagacct gaagccgcgc gacatcctca gcaaggagtc gttcgacaac gccatcgtca
     3961 cgctcatggc catgggcgga tccacgaacg cgctgattca tctcatcgcc atggcggggc
     4021 gcgcgggcgt caagcttccg atggagcgct ataacgagtt ctcggccagg accccccttc
     4081 tcgccaacgt acgcccttcc ggcgacaaat acctgatgga ggacttctat tacgcgggcg
     4141 ggctgcgcgc
//
LOCUS       DATDMP010000536         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1530123, whole genome shotgun sequence.
ACCESSION   DATDMP010000536 DATDMP010000000
VERSION     DATDMP010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530123"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..981)
                     /locus_tag="VI195_09380"
     CDS             complement(43..981)
                     /locus_tag="VI195_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6824641.1"
                     /translation="MRFFLTAMLVILVGTASGEAPRAAVSPPRAVLVTGASSGIGRKV
                     TERLAAGGYFVYATARKPEDLKALGAMKNVQALRLDVTQPADIAAAVETIGKAGRGLY
                     GLVNNAGVATVGSVANMSFDEFDLVMKVNAYGPVEMIRAFEPLLIERQGRIVNIGSIS
                     GILASQNLTAYAMSKHAVEALTDSLAAQLEPQGVRVSVIEPGNYNSNIARNALERIGA
                     DSGATHSPGYKLQADRSQYKEPDEVAAAVEQALSDPHPKRRYMVVPNQTEAEITIRKQ
                     IEQLVQLNEGQPYSYDRAALIRMLDEGLAGARPGTN"
     gene            complement(1132..1614)
                     /locus_tag="VI195_09385"
     CDS             complement(1132..1614)
                     /locus_tag="VI195_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824642.1"
                     /translation="MLPLQAIDRAALARLLAGYRLDLRLVGPGGRIPGSYWGDSEAGL
                     EAERLYARPDTPVHSILHEAGHYLCMSPERRAGLDTDAGGDDLEEEAVCYLQVLLAGR
                     LAGVGRERLLADMDAWGYSFRLGSARAWFEQDAAAAHEWLEEHGLIDTRGELTGELRR
                     "
     gene            complement(1661..2164)
                     /gene="lptE"
                     /locus_tag="VI195_09390"
     CDS             complement(1661..2164)
                     /gene="lptE"
                     /locus_tag="VI195_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY6824643.1"
                     /translation="MGCGRLRPAPAAAGLAAVALLAGGCGFHLEGRTALPAAVKTPYL
                     EAEDRQSDFVQSLRRALHASGAQLAPEKDKASAVVSILKDSVKQRVLSVSNLNQPTEY
                     ELTYTVSFAVSAGGHELLAPQEISTTRTYSFNERLLLAKGHEADVLRGAMAQDLADMV
                     LRRLSRL"
     gene            complement(2164..>2847)
                     /locus_tag="VI195_09395"
     CDS             complement(2164..>2847)
                     /locus_tag="VI195_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6824644.1"
                     /translation="LIERFGADTARLFTMYASPPEQTLEWSDEGVQGASRFIRRLWSA
                     VYEHVAAGAAPALEPRSLTPAQRALRRAAHQALAKATDDIGRRRNFNTAIAAMMELLN
                     SIRASADASPQGRAVRQEALDIEVRVLAPVTPHVCHALWHSLGHRTALVDERWPQPDP
                     AALEQETHAMVVQVNGRLRGHITVPVHADEAAVRAAALADEQVRRFVADKPVRRVIVV
                     PGKLVNVVV"
BASE COUNT          390 a         1010 c         1011 g          436 t
ORIGIN      
        1 gcgcgatcgg ccgggccggt gcgggcagcg gcgccgaggg cctcagttgg ttcccggccg
       61 cgccccggca aggccctcgt ccagcatcct gatcagggcc gcgcggtcgt agctgtaggg
      121 ctgcccttcg ttgagctgca ccagctgctc gatctgcttg cggatggtga tctcggcctc
      181 ggtctgattc ggcacgacca tgtagcggcg cttcggatgc ggatcggaaa gcgcctgctc
      241 cacggccgcc gccacttcgt ccggttcctt gtactgggag cggtccgcct gcagcttgta
      301 ccccggggaa tgagtcgcgc ccgaatccgc gccgatgcgc tcgagcgcat tgcgggcgat
      361 gttggagttg tagttgccgg gctcgatgac gctgacgcgc acaccctgag gctccagctg
      421 ggcggcgagt gaatccgtga gcgcctcgac ggcgtgtttg ctcatggcat acgcggtgag
      481 gttctggctg gcgagaatcc cggaaatcga gccgatgttg acgatccgcc cctgccgctc
      541 gatgagcagc ggctcgaacg ccctgatcat ctcgaccggc ccgtaggcgt tcaccttcat
      601 caccaggtcg aattcatcga agctcatgtt ggcgacgctg ccgacggtcg cgaccccggc
      661 gttgttcacc agcccgtaaa gaccacgccc ggctttgccg atggtctcga ccgccgcggc
      721 gatgtccgcc ggctgagtga catcgaggcg cagcgcctgc acgttcttca tggctccgag
      781 cgccttgaga tcctcgggct tgcgggcggt cgcgtagacg aaatagccgc cggcggcgag
      841 ccgctcggtg accttgcggc cgatgccgct gctggcgccc gtcacgagga ccgcccgcgg
      901 tggggacact gccgcccgcg gtgcctcacc ggaggcagta ccgaccagaa tcaccagcat
      961 cgcggtcagg aaaaatcgca tgacggaccc ccagcgttat cggccgaacc gtgcgccttg
     1021 atgcgttgcc agatgtcgcg ggcaaatccg cggtgcggcg ccggcgcctc cgcagcgggt
     1081 cgtggatcgg tcggtgccgg tgactcgctc atgactggcc gcaagtgtag ctcagcggcg
     1141 cagctcgccc gtgagctctc cgcgtgtgtc gatgagaccg tgctcctcga gccactcgtg
     1201 cgccgcggcg gcatcctgct cgaaccacgc gcgggcactg cccaggcgga agctgtagcc
     1261 ccaggcgtcc atgtcggcga gcagccgctc gcggccgacg cccgcgagcc gcccggcgag
     1321 cagcacctgc aggtagcaca ccgcctcctc ctcgaggtcg tcgccgcctg cgtcggtgtc
     1381 gagtcccgcg cgccgctccg ggctcatgca cagatagtgt ccggcctcgt gaaggatgga
     1441 gtgcaccggg gtatcgggcc gcgcgtagag ccgctcggct tcgagtcccg cctcactgtc
     1501 gccccagtac gagcccggga tgcgcccccc ggggccgacc agcctcaggt cgaggcgata
     1561 gccggcgagg aggcgcgcga gcgccgcccg gtcgatggcc tgcagcggca gcacggccga
     1621 tgcacacgcc gccggagcgt gaggcgcgcc ggacacgcgc ctagagcctc gacagccgcc
     1681 gcaacaccat gtcggcgagg tcctgggcca tcgccccgcg cagcacgtcg gcctcgtgac
     1741 ccttggcgag cagcagccgc tcgttgaagc tgtaggtgcg ggtggtggag atttcctgcg
     1801 gcgcgagcag ctcgtgcccg ccggcgctca ccgcgaagct caccgtgtag gtgagctcgt
     1861 actcggtcgg ctggttgagg ttggagaccg acagcacccg ctgcttcacg ctgtccttga
     1921 ggatgctcac caccgccgag gccttgtcct tctcgggcgc gagctgcgcg ccgctcgcgt
     1981 gcagcgcccg ccgcaggctc tgcacgaaat cgctctgccg gtcctcggct tcgagatacg
     2041 gggtcttgac ggccgcgggc agcgccgtgc gcccttcgag gtggaagccg cagccgccgg
     2101 cgagcagcgc gaccgccgcc agcccggcgg cggcgggcgc aggccggagg cggccgcagc
     2161 ccatcacacc accacgttca cgagcttccc gggaacgacg atgacccgcc gcaccggctt
     2221 gtcggccacg aacctgcgca cctgctcgtc ggcgagcgcc gcggcgcgta ccgccgcctc
     2281 gtcggcgtgc accggcacgg tgatgtggcc gcggagcctg ccgttcacct gcaccaccat
     2341 ggcgtgggtc tcctgctcga gcgccgccgg atcgggctgc ggccagcgct catcgaccag
     2401 cgccgtgcgg tggccgagcg agtgccacag cgcgtgacac acgtgcgggg tgacgggcgc
     2461 gagcacccgc acctcgatgt cgagcgcttc ctggcgcacc gcgcgaccct gcgggctggc
     2521 atcggccgag gcgcggatcg agttgagcag ctccatcatc gcggcgatgg cggtgttgaa
     2581 gttccgccgg cggccgatgt cgtcggtggc cttggcgagc gcctggtggg cggcgcggcg
     2641 cagcgcgcgc tgcgccggtg tgagcgagcg cggctcgagc gccggggccg cgcccgcggc
     2701 cacgtgctcg tacaccgcgc tccacaggcg gcggatgaag cgcgacgcgc cctgcacgcc
     2761 ctcgtcggac cactccaggg tctgctcggg tggcgaggcg tacatggtga aaagccgcgc
     2821 ggtgtcggcg ccgaagcgct cgatgag
//
LOCUS       DATDMP010000537         3992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1641828, whole genome shotgun sequence.
ACCESSION   DATDMP010000537 DATDMP010000000
VERSION     DATDMP010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641828"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VI195_09400"
     CDS             complement(<1..650)
                     /locus_tag="VI195_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824645.1"
                     /translation="MRTLFLHPPSFDGFDGGAGARYQMTREVRSFWYPTWLAQPAALV
                     EGSKLIDAPPQRLSFEDIIPELRSRELVVMHTSTPSFKADARTAARIKEVNPHVRIGL
                     IGAHVAVEPARSLAAAPAVDFVTHNEFDFTIKEVAEDRPLAQVKGLSWRNAAGAIVHN
                     EPRAPLANMDELPWVTPVYKRDLKVENYFSGYLKHPYVSFYTGRGCKSRCTFCLWP"
     gene            complement(661..1803)
                     /gene="hpnI"
                     /locus_tag="VI195_09405"
     CDS             complement(661..1803)
                     /gene="hpnI"
                     /locus_tag="VI195_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEY6824646.1"
                     /translation="MSAAQLWLGGLGTALAALAMGYAIAAALARRVGRAAPAQRSPTL
                     PAVTLLKPLCGAEPGLYEHLRSFCVQSYPCWQVVFGVRDPEDRALEAVRRLRSEFPHL
                     DLQVAIDPTQHGASSKVSNLINMMPLARHDSLVITDSDVIVERDYLERVITPLLDERV
                     GIVTCPYRGRPRPGLWSLLGSMFINEWFMPSVRVAAACGSRAFAFGATIALRRATLVE
                     IGGFAAIADQLPDDYRLGELTRRLGLRTVLSGVEVETCVAEASLSELLRHELRWLRTV
                     RTVRPVGYALAGITFAVPAALAGSVLAGGSVTTLVLVAITAAARLMVNSAPRSSYSLP
                     GQLGLVAVNDLLGFVLWCWSFATRRVHWRDGSYQLARDGSIHPIPH"
     gene            1893..2483
                     /gene="idi"
                     /locus_tag="VI195_09410"
     CDS             1893..2483
                     /gene="idi"
                     /locus_tag="VI195_09410"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEY6824647.1"
                     /translation="MTDQVRPAPVDAGNMVLDLEPLILVDEADREIGHLDRINCHAGS
                     GVLHRAFSLLIFNGNGELLLQQRAAGKRLWPLYWSNSCCSHPRRAESMEAAIHRRLLE
                     ELGIDCPLHFLFKFQYQAQFDAATAERELCSVFIGRCNGRVKANAAEVHDWRWISTAQ
                     LQSEMNGPEGRKFTPWFTLEWERIWREHRPAVLALR"
     gene            2508..3659
                     /gene="hpnH"
                     /locus_tag="VI195_09415"
     CDS             2508..3659
                     /gene="hpnH"
                     /locus_tag="VI195_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY6824648.1"
                     /translation="MLGIPLLQMYRVGRYVLTRKLRGEQRYPLVLMLEPLFQCNLACA
                     GCGKIDYPKEVLQQRMSVADALRAVDECGAPIVSIAGGEPLIHRELPQIVAGIVARRK
                     FVYLCTNAILLSRHIDEYRPSPYLTLSLHLDGNRDRHDESVCQEGVFDKVIAAIRLAR
                     ARGFRVTVNCTLFNGADPQEVAEFFDTAMQQGIEGITVSPGYSYSHAPRQDVFLARTA
                     SKSLFREIFRRGRQRAHARRWSFNHSALYLDFLAGNQTYQCTPWSNPTYNIFGWQRPC
                     YLLSDEGTASSYRALMEETEWERYGVGRNPRCDNCMAHCGYEGTAVTDALAHPLKALT
                     AAVRGPRLTGPMAPDPPLLYSEHARMGSVAVPLSAVKRAGETARAAELQ"
     gene            3656..>3992
                     /locus_tag="VI195_09420"
     CDS             3656..>3992
                     /locus_tag="VI195_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY6824649.1"
                     /translation="MIEGAGYATRVQAWQARMEQALAQRLPASDAEPTRLHAAMRYCV
                     LNGGERVRPVLLFGTARALGLTEAEVEAAACAIELIHAYSLVHDDLPALEDDEERGGR
                     PACHKAYDEG"
BASE COUNT          683 a         1307 c         1354 g          648 t
ORIGIN      
        1 tggggccaca ggcagaaggt acaacgcgat ttgcagccgc ggcccgtata gaacgaaacg
       61 tacggatgct tgaggtagcc gctgaaataa ttctcgacct tgagatcgcg cttgtagact
      121 ggcgtcaccc agggaagctc gtccatgttc gcgagcggcg cgcgcggctc gttgtgcacg
      181 atggcgcctg cggcgttgcg ccacgagagc cccttgacct gcgccagcgg ccggtcctcg
      241 gcgacttcct tgatggtgaa gtcgaactcg ttgtgggtca cgaaatctac cgccggcgcg
      301 gccgcgaggc tgcgcgcggg ctccaccgcc acgtgcgcac cgatgaggcc gatgcgtacg
      361 tgcggattca cctccttgat cctggccgca gtgcgcgcgt ccgccttgaa ggaaggcgtg
      421 gaggtatgca tcaccaccag ctcgcggctg cgcagctcgg ggatgatgtc ctcgaaactc
      481 agccgctgcg ggggcgcatc gatcagtttc gagccctcga ccagcgccgc cggctgcgca
      541 agccaggtcg ggtaccagaa cgagcgcacc tcgcgggtca tctggtagcg cgccccggcg
      601 ccgccgtcga agccgtcgaa agagggaggg tgcaggaaaa gtgttctcac ggccgcttac
      661 tcagtgggga atcgggtgaa tgctgccatc gcgagccagt tgataactgc cgtcgcgcca
      721 gtgcacgcgc cgggtcgcga aactccagca ccacaggacg aagccgagca gatcgttgac
      781 cgcgacgagt cccagctgcc ccgggagcga atacgagctg cgcggtgcag aatttaccat
      841 gaggcgcgcc gccgccgtga tcgccaccag taccagcgtg gtcacgctgc cgcccgcgag
      901 cacgctgccg gcgagcgccg ccggcacggc gaaggtgatg cctgcgagcg catagccgac
      961 gggccgcacc gtgcgcaccg tgcgcagcca gcgcaactcg tgccgcagca gctcgctcag
     1021 gctcgcctcg gctacgcagg tctcgacctc cacccccgac agcacggtgc gcaggcccag
     1081 gcggcgggtg agctcgccca gccgatagtc atcgggcagc tgatcggcaa ttgccgcgaa
     1141 cccgccgatc tcaacgagcg tggcgcggcg caaggcgatg gtggcgccga aggcgaaggc
     1201 acgcgagccg caagccgctg cgacgcgcac cgagggcatg aaccattcat tgatgaacat
     1261 cgagccgagc aacgaccaca gcccgggccg cgggcgcccg cggtaggggc aggtgacgat
     1321 gccgactctc tcgtccagga gaggcgttat cacccgttcg aggtagtcgc gctcgacgat
     1381 gacgtcgctg tcggtgatga ccagggaatc gtgacgcgcg aggggcatca tgttgatcag
     1441 gttgctgacc ttgctgctcg ccccgtgctg cgtcgggtcg atggcgacct gcagatcgag
     1501 atgtggaaac tcgctgcgca gtctgcgcac ggcttcgagc gcgcggtcct ccgggtcgcg
     1561 tactccgaaa acaacctgcc agcagggata gctctgcacg cagaacgagc gcagatgctc
     1621 gtacaagcca ggctccgcgc cgcacagcgg tttcagcagc gtgaccgccg gcagggttgg
     1681 cgagcgctgc gccggcgcag cgcggccaac acgcctggcg agcgcggcgg cgatggcata
     1741 gcccatcgcc aacgctgcga gtgcggtgcc gagcccaccg agccagagct gggcggcgct
     1801 catctcggca gcgactctgt ggcgagtacg ggtatcggag gtatcatcgt gcgcactgta
     1861 cacgtgcgct cggctggtgc ggagttagtt gcttgacgga tcaggttcgg cccgcgccgg
     1921 tggacgcggg caacatggtg ctcgatctgg aaccgctcat actcgtggac gaagctgacc
     1981 gcgagatcgg ccatctcgac cggatcaact gtcatgccgg cagcggtgta ctgcaccgcg
     2041 ctttctcgct gctgatattc aacggcaacg gtgagctgct gttgcagcag cgcgcggccg
     2101 gcaagcggct ctggccgttg tactggtcca acagctgctg cagtcatccg cggcgcgccg
     2161 agagcatgga ggctgccatt caccgtcgac tgctggagga gctcggcatc gattgcccgc
     2221 tgcacttcct gttcaagttc cagtatcagg cccagttcga tgccgcaacc gccgagcgcg
     2281 agctgtgctc ggtcttcatc ggccgctgta atggccgtgt caaggcaaac gccgccgagg
     2341 ttcacgactg gcgctggatc agtacggccc agctccagtc agagatgaac ggacccgagg
     2401 gccgcaagtt taccccctgg ttcactctcg agtgggaacg aatctggcgc gagcaccgcc
     2461 cggcggtgtt ggcgctgcgc taatcttggc gggtcactga ggagggcatg ttgggtatcc
     2521 ccctgctgca aatgtatcgc gtcggccgct acgtgctcac ccgcaagctg cggggcgagc
     2581 agcgttaccc gctggtgctg atgctcgagc cgctgttcca gtgcaacctc gcctgcgcgg
     2641 gctgcggcaa gatcgactat ccaaaggagg tgctgcagca gcgcatgagc gtcgccgacg
     2701 cgctgcgtgc cgtcgatgaa tgcggtgcgc cgatcgtctc cattgccggc ggcgagccgc
     2761 tgatccaccg cgagctgccg cagatcgtgg ccggcatcgt ggctcgcagg aagttcgtct
     2821 atctctgtac caacgccatt ctgctgtcgc gccacatcga cgagtaccgg ccctcgccct
     2881 atctgacgtt gtcgctgcac ctcgatggca atcgcgaccg tcacgacgag tcggtgtgcc
     2941 aggaaggcgt gttcgacaag gtgatcgcag ccatccggct ggcgcgcgcc cggggcttcc
     3001 gcgtgacggt caactgcacg ctgttcaacg gcgccgaccc gcaggaggtg gcggagttct
     3061 tcgacacggc catgcagcag ggcatcgagg gtatcaccgt ttcgcccggc tacagctaca
     3121 gtcatgcgcc gcggcaggat gtgttcctgg cacgcaccgc gagcaaatcg ctgtttcgcg
     3181 agatcttccg ccgcggccgg cagcgcgcgc atgcgcggcg ctggtcgttc aaccattcgg
     3241 cgctgtatct cgacttcctg gccggtaacc agacttacca gtgcactccc tggagcaatc
     3301 ccacctacaa catcttcggc tggcagcgcc cctgttacct cctcagcgac gaaggcacgg
     3361 ccagcagcta tcgcgcactg atggaggaga ccgaatggga gcgttacggc gtgggacgca
     3421 acccccgctg cgacaactgc atggctcact gcggctacga aggtactgcg gtcaccgacg
     3481 ctctggcgca tccgttgaaa gcgctcactg cggcagtgcg cggtccgcgc ctgacggggc
     3541 ccatggcacc cgacccgccg ctcctgtaca gcgaacacgc gcgcatgggc agtgtcgcgg
     3601 tgccgctgag cgccgtgaag cgtgccggcg agacggcgcg ggcggcggag ctgcagtgat
     3661 cgagggcgcg ggctacgcca cccgggtgca ggcgtggcag gcgcgcatgg agcaggcgct
     3721 ggcgcagcgc ctgccggcga gcgacgcaga accaacacgc ctgcacgcgg cgatgcgtta
     3781 ttgcgtgttg aacggcggcg agcgggtacg tccggtgctg ctgttcggta ccgcccgcgc
     3841 tctcgggctc accgaagccg aggtcgaggc ggcggcgtgc gccatcgagt tgatccacgc
     3901 ttactcgctc gtacacgatg acctgccggc tctggaggac gatgaggagc gcggcggccg
     3961 gccggcctgt cacaaggcct acgacgaagg ca
//
LOCUS       DATDMP010000538         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_420612, whole genome shotgun sequence.
ACCESSION   DATDMP010000538 DATDMP010000000
VERSION     DATDMP010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420612"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..412)
                     /locus_tag="VI195_09425"
     CDS             complement(14..412)
                     /locus_tag="VI195_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6824650.1"
                     /translation="MADKREVASQESSAVERQGREREAALVPAVDIFEDAQQITVQAE
                     MPGVSKNKLNVQADRNALLIEGEMEVDLPAGMAALYADLHTTRYRRSFVLSGELETER
                     IEASLKDGLLTVRIPKRLEFRTRKIRVNVP"
     gene            complement(430..876)
                     /locus_tag="VI195_09430"
     CDS             complement(430..876)
                     /locus_tag="VI195_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6824651.1"
                     /translation="MFERLLSGIDTDLFEHFRRLEEEVGQVFGHAPWPSGIRAVQRGT
                     FPPINVGATPERVDVYLFAAGLDPKSLDLSIQQNLLTVAGSRRIGTNEQAEYYRRERF
                     DGDFRRVITLPDDVDPERVEARYRDGVLQVTIQRREAARPRQISVT"
     gene            1042..1320
                     /locus_tag="VI195_09435"
     CDS             1042..1320
                     /locus_tag="VI195_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824652.1"
                     /translation="MQIRNSPGMAVAAAAAVLFGTGLVGTAFAGSEAKIQCAGVNACK
                     GQSACKGANNACKGQNSCKGKGFLEMTEKECADAQERARAILQPGEGR"
     gene            1385..1768
                     /locus_tag="VI195_09440"
     CDS             1385..1768
                     /locus_tag="VI195_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6824653.1"
                     /translation="MKRMLGSLALLLALVAAPAFGAEVPYDAAAFQKLLAGGRPVAVD
                     FYASWCPTCRAQAPLLKSLAAEPQFASLTLLVADFDIEKALCKSLNVTEQSTLIVFRN
                     GKEVARSTGDTSRAGLTRLLLAALR"
     gene            1782..>2338
                     /locus_tag="VI195_09445"
     CDS             1782..>2338
                     /locus_tag="VI195_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY6824654.1"
                     /translation="MTFGTGSYALSALAGVLTTLSPCVLPLIPILVSAAVATHRLGPL
                     ALAAGVASSFTALGVLLAAFGAALGLDADTFRTAGAILFIAIGVLLVSGRLQAEFARA
                     TSRLSGAGATLLARCTLDGIAGQFALGLLLGVVWTPCVGPTLGAAVTLASRGEDLVQI
                     TFLMAVFGVSASVPLLAIGLASRAAL"
BASE COUNT          331 a          818 c          778 g          411 t
ORIGIN      
        1 ccggagcccc gggtcaaggc acgttcaccc tgatcttgcg cgtgcggaac tcgagccgtt
       61 ttggaatgcg caccgtgagc aagccgtcct tgaggctcgc ttcgatgcgc tcggtctcga
      121 gctccccgct caggacgaag ctgcgccggt agcgggtggt atgcagatcc gcgtacagcg
      181 ccgccatgcc cgccggcaga tcgacctcca tctcgccctc gatgagcagg gcgttgcggt
      241 cggcctgcac gttgagcttg ttcttcgaga cgccgggcat ctcggcctgc accgtgatct
      301 gctgcgcgtc ctcgaagatg tcgaccgccg gcacgagcgc cgcttcgcgc tcgcgcccct
      361 ggcgctctac ggcgctggat tcctgactcg ccacctcgcg tttgtcagcc atggcttctg
      421 ccctcctgtt caggtcacgg agatctgccg cggccgggcg gcttcgcggc gctggatggt
      481 cacctgcagc accccgtcgc ggtacctggc ttcgacccgc tcggggtcga cgtcgtccgg
      541 cagcgtgatc acgcggcgga agtctccatc aaagcgctcg cgccggtagt actcggcctg
      601 ctcgttcgtg ccgatgcggc ggctgccggc taccgtcagc aggttctgct ggatcgacag
      661 atcgaggctc ttcggatcga gtcccgcggc gaacaggtaa acgtcgaccc gctcgggggt
      721 cgccccgaca ttgatcggtg ggaacgtgcc acgctgcacc gcacgaattc cggacggcca
      781 gggtgcgtgg ccgaacacct ggcccacctc ctcctcgagc cgcctgaagt gctcgaacag
      841 atcggtgtca atgccgctca acagcctctc gaacatggcg aacacctcct gtgggttggg
      901 tagatgacgg caaggtaggt tctccggccg gggattcaag ggccgtcgga caccccggcg
      961 caagaaatgc caccgggccc cggtctactg ggctgaccgg atagcttccg gtctcagtca
     1021 ccactttacg aggaatgccc catgcaaatc agaaactccc ccggcatggc cgttgccgct
     1081 gccgccgccg tcctgttcgg caccggactc gtcggcaccg ccttcgccgg ctccgaagcg
     1141 aagatccagt gcgccggcgt caacgcctgt aagggccaat cggcgtgcaa gggcgccaac
     1201 aacgcctgta agggccagaa ctcctgcaaa ggcaagggtt tcctcgagat gacggaaaag
     1261 gagtgtgcgg acgcgcagga aagagcgcgt gctatcctgc agccgggcga aggccggtaa
     1321 gggtcaccgc gtgtcgcgcg ccggcacgcg gccggcgcgc ggctttccgc tcgaggagag
     1381 gccgatgaaa cggatgctcg ggtccctggc gctgctgctc gcgctcgtgg ccgccccggc
     1441 cttcggcgcc gaggtaccct acgatgccgc cgccttccag aaactgctgg ccggcggtcg
     1501 gcccgtcgcg gtggatttct acgcgagctg gtgccccacc tgtcgcgccc aggcgccgct
     1561 gctcaagtcg ctcgccgccg agccgcagtt cgccagcctc acgctgttgg ttgccgactt
     1621 cgacatcgag aaggcgctct gcaagtcgct caacgtgacc gagcagagca cgctgatcgt
     1681 gttccgcaac ggcaaagagg tcgcccggtc gacgggcgat accagcaggg ccggactcac
     1741 gcgactgctg ctcgctgcac tccgctgaaa tcagccgcgg cgtgacgttc ggcaccggca
     1801 gctatgcatt gagcgcgctc gccggcgtgc tcactacgct ctcgccgtgc gtcctgccgc
     1861 tcatcccgat tctcgtgagc gccgcggtgg ctacccaccg cctcgggccg ctggcgctcg
     1921 ctgccggcgt cgcgagctcc tttacggcgc tcggggtgct gctggcggca ttcggcgctg
     1981 cgctcggact cgatgccgat accttcagga cggcgggggc gatcctgttc atcgccatcg
     2041 gcgtgctgct ggtctcgggc cggctgcagg ccgagttcgc ccgggcaacg agccgcctct
     2101 ccggcgccgg cgccacgctg ctcgcgcgct gcacactcga cgggatcgcc gggcagttcg
     2161 ccctggggct cctgctcggt gtggtgtgga ctccgtgcgt cggtcccacg ctcggtgccg
     2221 cggtgacgct cgccagccgc ggcgaggatc tcgttcagat cacctttctc atggcggtgt
     2281 tcggggtgag cgcgagcgtg ccgctgctcg cgatcggact ggcgtcgcgc gcggcgct
//
LOCUS       DATDMP010000539         4683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_309633, whole genome shotgun sequence.
ACCESSION   DATDMP010000539 DATDMP010000000
VERSION     DATDMP010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309633"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VI195_09450"
     CDS             <1..464
                     /locus_tag="VI195_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY6824655.1"
                     /translation="SSNLATNLLMDKLGVDNIRAGVHALGADGMNVRRDLEDGKAFAQ
                     GLNNNTTASALQQLLEAIARGHAVDEASSREMLAVLERQTVNDRIPAGLPAGTRVAHK
                     TGEITGIRHDAAIVFAGRPFVLVVLTRGAASPEAGSKLIAEITRQLYRATQ"
     gene            635..2689
                     /locus_tag="VI195_09455"
     CDS             635..2689
                     /locus_tag="VI195_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY6824656.1"
                     /translation="MSMARRLLWVVVAVLGAVALGIVALKRGEPVNAMWLVIAALCIY
                     AIAFRFYGLFIANRVLGVSAARQTPAYRHNDGLDYVPTNGYVLFGHHFAAIAGAGPLV
                     GPVLAAQMGYLPGTLWLLAGVVFAGAVQDMTVLFLSTRRDGRSLGDLIRSEMGPLAGT
                     VAGIGVLLICVIILAVLALVVVNALKSSPWGTFTVACTIPIALIMGIYGRLVRPGRVG
                     EMSALGGVLLLAALIYGRKVSETPELAHYFTFSGPALALLIIAYGFVASVLPVWLLLA
                     PRDYLSTFLKVGTVALLAIGVLIAHPQMQMPAITRFIDGTGPVWAGSVFPFLFITIAC
                     GAVSGWHSLISSGTTPKMVESEKQIAFIGYAGMLTESFVAIMAMIGASIIHPGVYFAM
                     NSGAALIGNTAAHAADVITQWGFTVTPDMLTQVAHDVGESSILSRTGGAPTLAVGMAT
                     IFSSFLGGRALMGIWYHFAILFEALFILTTVDAGTRVCRFMIQDLVGHAIPAFKATRA
                     WGNNVIGSALSCVCWGYLLYAGVVDPYGGIWTMWPLFGATNQMLAAIALTLCTVVLYK
                     MKRERCAWVTIVPTVWLVVCTVTAGLEKVFSPTPKVGFLSHAEKFSQALAAQQVLAPA
                     KSLQEMSRVIFNDYLDAALAALAVAIVIVVVAYGIISIRQARATPRNTAMEIGEAALG
                     HG"
     gene            2682..2882
                     /locus_tag="VI195_09460"
     CDS             2682..2882
                     /locus_tag="VI195_09460"
                     /inference="COORDINATES: protein motif:HMM:NF016234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CstA-like transporter-associated
                     (seleno)protein"
                     /protein_id="HEY6824657.1"
                     /translation="MDRLVHLWRRAVQTARLMVGIPDYDTYVAHRRTHHPEAPVMSYA
                     EFFRERQQARYGCEGGRIGGCC"
     gene            3128..3946
                     /locus_tag="VI195_09465"
     CDS             3128..3946
                     /locus_tag="VI195_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824658.1"
                     /translation="MEEALQPDAMKVAQQRGASELACPAATSELLSKRAVQEAQSTGW
                     YEAPRRAEYTVAVSGCGKRTTYLVACNKFRSACEAGAVPAPTRSGALEKLAEELQPDA
                     LKAAQQRGARDFTCPTTTAEVTHRETIVEGQTTGWDAEAPHRAVYAVTVKGCEKQTAY
                     LVECDKRKKACASGGLEQAQATPQHPQLADELQPGAVQTAQERGSKELECPAATAVVN
                     RKATIDEGTTTGWYEAPFRAAYTITVAGCGKQTTYLVACNKKEKRCGARSPSSQ"
     gene            complement(3973..>4683)
                     /locus_tag="VI195_09470"
     CDS             complement(3973..>4683)
                     /locus_tag="VI195_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824659.1"
                     /translation="APGPDRRLASESFRQFFGEVIGVVRRPQVLLAIVMFVGPASSFA
                     LTNLLGGLGSDFGASAHFVGAIGGAGVLCAGIAGCLVFPLIDRLLPLRYLYLAIGGVG
                     ALFTLALLLLPRTPLAFATALIGQNLFQSAAITTAIAIAFETIGQHNPLAATTFSVIV
                     SVLNIPNTYMVVVDGWGYAWRGVDGSLLVDAGASLVMCGFLILLLGYLSLRRRAAAAV
                     TSPPELPAPAAASDDALW"
BASE COUNT          831 a         1514 c         1547 g          791 t
ORIGIN      
        1 tgagcagcaa cctcgcgacc aacctgttga tggacaagct cggtgtcgac aacatccgtg
       61 ccggcgtcca cgcgctcggt gccgatggca tgaacgtacg gcgcgatctc gaggacggta
      121 aggcgttcgc gcaggggctc aacaacaaca ccaccgcgag cgcgctgcag cagctgctgg
      181 aggccatcgc ccgcggtcac gccgtggatg aggcgtcatc gcgggagatg ctcgcggtgc
      241 tcgaacgcca gacggtgaac gaccgcattc ctgccggact ccccgccggt acgcgcgtcg
      301 cccacaagac cggggagatc accggtatcc ggcacgatgc ggcgatcgtc ttcgcgggca
      361 gacccttcgt gctggtggtg ctcacgcgcg gggcggcgag ccccgaggca ggctccaagc
      421 tcatcgccga gatcacccgg cagctctacc gcgcgacaca gtagcctgac gaccggcaag
      481 cgctggcggt gccattgccg tgtggcgagg cctgccgtta agcgctaatg tcccgtccgt
      541 tgctgcaggc ggtccaaaac aactggtgcc agggctctcc ccgagcgcgc gggatcggcc
      601 gctgagccac ctacaagagc cgtaagagcc aacgatgtcg atggcaagaa gactgctttg
      661 ggttgtcgtc gcggtgctcg gtgccgtggc gctgggcatc gtcgcactga agcgcggcga
      721 gccggtgaat gcgatgtggc tggtgatcgc ggcgctgtgt atctacgcga tcgccttccg
      781 gttctatggt cttttcatcg ccaacagagt tctcggggtg agcgcggcac gccagacacc
      841 cgcgtatcga cacaacgacg gcctcgatta cgtgccgacc aatggctacg tgctcttcgg
      901 tcaccatttc gccgcaatcg ccggcgccgg accgctcgtg ggaccggtgc tcgccgcaca
      961 gatgggctac ctgccgggca cgctctggct cctggccggg gtggtattcg cgggcgccgt
     1021 gcaggacatg accgtgctgt tcctctcgac gcgccgcgac ggccgctcac tcggagacct
     1081 gattcgctct gagatgggcc cgctcgccgg cacggtcgcg gggatcggcg tgctgctcat
     1141 ctgcgtgatc attctcgcgg tgctcgcgct cgtcgtggtg aatgccctca agagcagccc
     1201 ctggggtacc ttcacggtcg cctgcaccat cccgatcgcg ctcatcatgg gcatctacgg
     1261 ccgcttggtg cgcccgggac gggtcggcga gatgtccgca ctcggtggcg tgctgctgct
     1321 cgccgcgctc atctacggac gcaaggtctc cgagacgccg gagctcgcgc actacttcac
     1381 gttctccggg ccggcgctgg ccttactcat cattgcctat ggttttgtcg cctcggtgct
     1441 gcccgtgtgg ctgctgctcg cgccgcgcga ctatctctcg accttcctca aggtcggcac
     1501 ggtggcgctg ctcgccatcg gcgtgctcat cgcccatccg cagatgcaga tgccggcgat
     1561 cacccgcttc atcgacggca cggggccggt gtgggccgga agcgtgtttc ctttcctttt
     1621 catcaccatt gcctgcggcg cggtgtccgg ctggcactcg ctcatttcct cgggcacgac
     1681 gccgaagatg gtcgagagcg agaagcaaat tgccttcatc ggctacgccg gtatgttgac
     1741 cgagtcgttc gtcgccatca tggcgatgat cggcgcctcg atcattcatc cgggtgtgta
     1801 ttttgcgatg aacagcgggg cggcgttgat cggcaacacc gccgcgcacg cggccgatgt
     1861 catcacgcag tggggcttca ccgtgacacc cgacatgctg acgcaggtgg cgcacgatgt
     1921 cggtgagagc agcatcctct cgcgcaccgg tggggcgccg acgctcgctg tcggcatggc
     1981 cacgatcttc tcgagcttcc tcggcggccg cgcgctgatg ggcatctggt accacttcgc
     2041 cattctgttc gaggcgctct tcatcctgac caccgtcgat gcaggcacgc gcgtgtgccg
     2101 cttcatgatc caggatctcg tcgggcatgc gattccggcc ttcaaggcca cccgtgcctg
     2161 gggcaacaac gtcatcggct cggccctttc ctgtgtctgc tggggttatc tgttgtacgc
     2221 gggggtggtc gatccctacg gcggcatctg gaccatgtgg ccgctgtttg gcgccaccaa
     2281 ccagatgctg gcggcgatcg cgctgacgct gtgcaccgtg gtgctctaca agatgaagcg
     2341 cgagcgctgt gcctgggtga cgatcgtgcc tacggtatgg ctggtggtgt gcacggtgac
     2401 cgccggactc gagaaggtct tcagccccac tccaaaggtc ggctttctga gtcacgccga
     2461 gaagttcagc caggcgctcg ccgcgcaaca ggtgctggca cctgccaagt cattgcagga
     2521 gatgagccgt gtgatcttca acgactatct cgatgcggcg ctggcggcgc tcgccgtcgc
     2581 tatcgtgatc gtggtggtgg cttacggaat catcagcatc cgtcaggcgc gggcgactcc
     2641 gcgcaacacc gcgatggaga tcggcgaggc ggcgctgggc catggataga ctcgtacatc
     2701 tgtggaggag ggcggtgcag accgcccgcc tgatggtagg gattccggac tacgacacct
     2761 atgtcgctca ccggcgcacg caccatcccg aagctccggt catgagctac gcagagttct
     2821 tccgcgaacg ccagcaggcg cgctacggct gcgaaggagg gcgcataggc ggctgctgct
     2881 gaggtcgcac tagctgcatg ggtccgttct cgggccaccc tggactaacg ggcagttgac
     2941 ccacccttga ctttcagagc actctcctcg cgcccagggg agaccatgag aaacagcctt
     3001 tcatcagggc ggattcgccg cggcaccact cttcgggcaa gggcagggcg ctgacatcat
     3061 gaggaagtca ctcgctttca gcgcgagcac actcaccgcg ctcctcatcg gctgtgcgtc
     3121 gcagccggtg gaggaagcgc tgcaacctga cgctatgaag gttgcgcagc agcgcggtgc
     3181 atccgagtta gcatgtccgg ccgccacaag cgaactcctc agcaagcgcg ccgttcagga
     3241 ggcgcagtcg acgggttggt acgaggctcc gcgtcgtgcc gagtacacgg tcgccgtctc
     3301 cggctgcggc aaacggacta cctacctggt cgcctgtaac aagtttcgca gcgcgtgtga
     3361 ggctggagcc gtgccagccc cgacccgctc gggcgcgttg gaaaagctgg cggaagaact
     3421 tcagcccgat gcgctaaagg cagcgcagca acgcggtgcg agagacttca catgcccgac
     3481 cacgacggcc gaggtgacgc atcgtgaaac gatcgtcgag ggtcagacca cgggctggga
     3541 cgccgaagcc ccacaccgcg ccgtgtatgc ggtgacggtg aagggatgcg agaaacagac
     3601 cgcctacctg gtcgagtgtg acaagcgcaa gaaagcctgc gcgagcggtg gcctggagca
     3661 ggcgcaagct acgccgcaac atccacaact ggccgatgag ttacagccgg gtgccgtgca
     3721 gaccgcgcag gaacgcgggt cgaaggagct cgaatgtccg gccgccacgg cagtagtgaa
     3781 ccgcaaggca accattgacg agggaacgac aaccggttgg tacgaggctc cgttccgcgc
     3841 cgcgtacacc atcaccgtcg ccggatgcgg caagcagaca acctacctcg tcgcctgtaa
     3901 caagaaagag aaacgctgcg gcgcgagaag tccgagtagc caataggcct gcacgatcgc
     3961 aacggccggg cgtcaccaca acgcatcatc actggccgct gcgggcgcgg gaagctccgg
     4021 cggtgaagtc accgcagccg cggcccggcg gcgcagcgac aggtatccga gcaacaggat
     4081 gaggaagccg cacatgacta gactcgcgcc ggcgtcaacg aggaggctgc cgtcgactcc
     4141 gcgccacgcg tagccccagc cgtcgacgac caccatgtag gtgttgggga tgttgagcac
     4201 cgacacgatc acgctgaacg tcgtcgcggc gagcgggttg tgctgaccga tcgtctcgaa
     4261 ggcgatcgcg atggcggtag tgatggccgc ggactggaac aggttctggc cgatcagcgc
     4321 cgtggcgaag gccagcggtg tacgtggcag cagcagcagc gcgagagtga acaacgcgcc
     4381 gacgccgcca atcgccaggt acaagtagcg caggggcagc agccggtcga tgagtgggaa
     4441 taccaggcaa cccgcgatgc cggcgcacag cacgcccgcg ccaccgatcg cgccgacgaa
     4501 atgcgccgat gcgccgaaat cgctgccgag tccgcccagc aggttggtga gcgcgaaaga
     4561 ggaagccggc cctacgaaca tcacaatcgc cagcagcacc tgcggacggc gaacgacgcc
     4621 gatcacctcg ccgaagaact ggcgaaagct ctcactcgcg aggcgccggt cgggccccgg
     4681 ggc
//
LOCUS       DATDMP010000540         2422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_864810, whole genome shotgun sequence.
ACCESSION   DATDMP010000540 DATDMP010000000
VERSION     DATDMP010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864810"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..581
                     /locus_tag="VI195_09475"
     CDS             357..581
                     /locus_tag="VI195_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824660.1"
                     /translation="MKRKGAEGFPARERLTPAANEEFTGTVNDLSRPELGRRDLDRLA
                     TPRSGWDPYEVWRTRVKASAKEKRGREPLR"
     gene            1050..2039
                     /locus_tag="VI195_09480"
     CDS             1050..2039
                     /locus_tag="VI195_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6824661.1"
                     /translation="MNALSVRGLTKTYRNGVPALRGIDLEVERGDFFALLGPNGAGKT
                     TLIGIITSLVNKSAGSASVFGHDIDRELEAAKACIGVVPQEINFNMFETPFTIVVNQA
                     GFYGIPRTVARQRAEKYLKQLQLWDKRHSMSRGLSGGMKRRLMIARALMHEPRLLILD
                     EPTAGVDIEIRRSMWDFLREINTRGTTIILTTHYLEEAETLCRNIAIINGGLIVERDR
                     MSSLLRRLHVETFVFNLREPLALAPPLPGYVTALVDDHTLEVEVSKEENLNDIFARLS
                     ALGIEVLSMRNKVNRLEEIFMQLVEGRAGAGDALRNGGALAAAARGQARAPSP"
     gene            2039..>2422
                     /locus_tag="VI195_09485"
     CDS             2039..>2422
                     /locus_tag="VI195_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824662.1"
                     /translation="MSSVVQPAAAPIRLDVVRWIGFKTIVIREYGRIIRIWAQTIVPS
                     VVTAVLYFAIFGSVFGRRVGSVDGFDYKQYIAPGLIMMSVITNSYGNVVASFFGAKFG
                     KHVEELLVSPLPNWVIVGGYAVGGVV"
BASE COUNT          445 a          802 c          781 g          394 t
ORIGIN      
        1 cgacgagcag ctgcgcgagt tctcccgcga agcccgcgaa tcactcgcag cgcagcactc
       61 catcgagtcc gccgaccggg gaacgttcga ggagtacctg gtccgttact tcgcggattg
      121 aaagtaaccc aacgtgaaaa aacttcatgt gcgggtcggc cgacataacc ttcggattaa
      181 gctgacacaa cttgtaatac gtctgacata actttaacat aatcaggcac ttcgtcagtt
      241 agcttttggc agacggacga catagactct gtgagccgcg tcgcactatg gatggcgctc
      301 gcggccgaag gaagcgacat aaaaggtccg gtcgacataa gcaggagcgc gagcagatga
      361 aaaggaaagg cgcggaagga ttccccgcac gagagcgact gaccccggcg gcaaacgagg
      421 aattcaccgg cacggtgaac gatctgagca gacccgagct cggccgacgt gatctggaca
      481 ggctcgcaac tccgcggtca ggttgggacc cgtacgaggt ctggcgtacg cgcgtgaagg
      541 cgtccgcgaa ggagaaacgc ggacgcgagc cgttgcgctg atgccgtaat cgatcgccgc
      601 agggggctca ctgccgctgc tgttggcggc cgttctattt tttatttcat gctcggcatc
      661 tcaccttcga cgccgcgttg cgcagcaacg gcgcgctgcc catcgggctc ctcaaccgcg
      721 cggccctgaa gcacagggac gcaacctgcg gcaacacgca cagctcgggc agctggcggt
      781 gtccccgctc taggccacca aggcatgcgg ccgcgacggc tgatcgtgcg acggacccgg
      841 gaacgacgcg tcgcccgccg cgctcgatga gcggcaggcg gccgagctgc tgaagaccgc
      901 gagttgactc accgcgggcg gcgcagcgcc gtcaataccc cgcccggggc atggtgcccg
      961 cgcaggccgc ccgcgacatt ggacgacgcg gccgccgctc gcgttgccct cacttgcacc
     1021 ggcgcgcgcc agccccgatc ctgcccgtca tgaatgccct ctcggtacgc ggactcacca
     1081 agacttaccg caacggcgtg ccggcgctgc gcggcatcga tctggaggtc gagcgcggcg
     1141 acttcttcgc gctgctcggt cccaatggcg ccggcaagac cacgctcatc ggcatcatca
     1201 cctcgctcgt gaacaagagc gccggcagcg ccagcgtctt cggccatgac atcgatcgcg
     1261 agctcgaggc cgccaaggcc tgcatcggcg tcgttccgca ggagatcaac ttcaacatgt
     1321 tcgagacgcc cttcaccatc gtggtgaacc aggcgggctt ctacggcatt ccgcgcacgg
     1381 tggcgcgcca gcgcgccgaa aagtacctca agcagctgca gctctgggac aagcgccaca
     1441 gcatgtcgcg cggcctgtcc gggggcatga agcgccgcct gatgatcgcg cgcgcgctga
     1501 tgcacgagcc gcggctgctg attctcgatg agcccaccgc cggcgtcgac atcgagatcc
     1561 gccgctcgat gtgggatttc ctgcgcgaga tcaacacgcg cggcaccacc atcatcctca
     1621 ccacgcatta cctggaggag gccgagacgc tgtgccgcaa catcgccatc atcaacggcg
     1681 gcctcatcgt cgagcgcgac cgcatgagca gcctgctgcg ccgcctgcac gtcgagacct
     1741 tcgtgttcaa cctgcgcgag ccgctcgcgc tggcaccgcc gctgccgggc tacgtgaccg
     1801 cgctggtcga tgaccacacc ctggaggtcg aggtctcgaa ggaggagaac ctcaacgaca
     1861 tcttcgcgcg gctttcggcg ctcggcatcg aggtgctgag catgcgcaac aaggtgaacc
     1921 gcctggagga gatcttcatg cagctggtcg agggccgcgc cggggcaggc gatgcgctgc
     1981 gcaatggcgg cgcgctagcg gccgccgcgc gcggccaggc gcgtgcgccg agcccgtgat
     2041 gagcagcgtg gtgcaacccg ccgcggcgcc gatccggctc gatgtggtgc gctggatcgg
     2101 cttcaagacg atcgtaatcc gcgaatacgg acgcatcatc cgcatctggg ctcagaccat
     2161 cgtcccctcg gtggtgaccg cggtactgta tttcgcgatc ttcggcagcg tattcggtcg
     2221 gcgcgtcggc agcgtggacg ggttcgacta caagcagtac atcgcacccg ggctgatcat
     2281 gatgtcggtg atcaccaact cctacggcaa cgtcgtggcc tcgttcttcg gcgccaagtt
     2341 cggcaagcac gtcgaggagc tgctggtgtc gccgctgccg aactgggtga tcgtcggcgg
     2401 ctacgccgtg ggcggcgtgg tg
//
LOCUS       DATDMP010000541         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_420771, whole genome shotgun sequence.
ACCESSION   DATDMP010000541 DATDMP010000000
VERSION     DATDMP010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420771"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="VI195_09490"
     CDS             <1..1701
                     /locus_tag="VI195_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HEY6824663.1"
                     /translation="KICTRPAHRRARAGSHGKDELEGPTRMTGWMRSRAVLAAVLAIA
                     AVTLLAAPRATQAALRVCADPGNMPLSNSRGEGLENKLAEVLARALGTSVAYYYRPGV
                     ERGLIRNTLDAEQCDVMLDMPIDSDDVLMTTALYRTTFVLAYRNDRGLDLKSLDDPRL
                     KTLKVGVYETSSIREALADHGVGKVEVHYLSHNADLVPENQPSYQIQQVIDGKLDVAA
                     AWGPLAGYYKAVKQAPLTIQPANLMDDYTPLQFDLGLAVRRNDHELQRRLEQAMHEQR
                     DALRAVLVAFGVPLVSCDTCVVSGDLPAHGPYKAREPAPATHAAPAVSIAQLNDWLAH
                     GANVNTELNNAVLADDKVRVSYLLDKKHASLSALDPQGETPLHHALQLRSTEMVTFLL
                     ARGADVNQRDRDGWTPLMTAAYGDDAADVKLLIAHGADANAVSAQNFTPLGIATQYDK
                     SKAALALIEGGADPARAIGDAGYTPLMLATANHDAQVARALIQKGADVNAHNSGGVTA
                     LMIAAAGSRADMVELLVQAGANVQAQTERGETALSIARTKGDEKVIRLLDGAAAHPGA
                     "
     gene            1753..>2028
                     /locus_tag="VI195_09495"
     CDS             1753..>2028
                     /locus_tag="VI195_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824664.1"
                     /translation="MTISILGLATITVLYAQPQTPPPAAAPPPAAGAPAGAAGAGGAT
                     MTPVQRADATPKGQLKNPYSDSDATVVQAGQKLYMNYGCSGCHGGTGG"
BASE COUNT          363 a          684 c          688 g          293 t
ORIGIN      
        1 aagatctgca cgcgcccggc gcaccgccgg gcgcgtgccg gatcgcacgg caaggacgag
       61 ctcgagggtc cgacgcgaat gacaggatgg atgcggagcc gggcagtgct cgctgcggta
      121 ctggccatcg cggcggtcac gctgctggct gcgccgcgcg cgacgcaggc ggcactgcgc
      181 gtgtgcgccg atccgggcaa catgcccctt tccaacagtc gcggcgaggg tctggaaaac
      241 aaactcgccg aagtactcgc gcgcgcgctg ggcacgagtg tcgcctacta ctaccgccct
      301 ggggtcgagc gcggactgat ccgcaacacg ctcgatgccg agcaatgcga cgtaatgctc
      361 gacatgccga tcgactccga cgatgtgttg atgaccacgg cgctgtatcg caccactttc
      421 gtactcgctt accgcaacga ccgtggcctc gacctcaaga gtctcgacga tccgcgcttg
      481 aagactctga aggtcggggt gtacgagact tcctcgatcc gtgaagccct ggccgatcat
      541 ggggtgggca aggtcgaagt ccactatctg agccacaacg ccgacctggt tcccgagaat
      601 cagccgagct atcagatcca gcaggtgatc gacggcaagc tcgatgtcgc cgccgcctgg
      661 gggccgctcg ccggctacta caaggcggtg aagcaggcgc ccttgaccat ccagcccgcc
      721 aacctcatgg acgactacac gccgctgcaa ttcgacctgg gcctggccgt gcggcgcaac
      781 gatcatgagc tgcagcgccg gctcgagcag gcgatgcacg agcagcgcga cgcattgcgg
      841 gcggtgctgg tggcattcgg tgtgccgctg gtgagctgcg acacctgcgt ggtgagcggc
      901 gatctgcccg cccacggtcc ctacaaggcg cgcgagcctg cgcctgccac gcacgccgct
      961 cccgcggtca gcatcgcgca gctgaacgat tggctggcgc acggcgccaa tgtcaacacc
     1021 gagctcaaca acgcggtgct cgcggacgac aaggtgcgcg tcagctatct gctggacaaa
     1081 aagcacgcct cgctgagcgc gctcgatccg cagggtgaga cgccgctgca tcacgcgctg
     1141 cagctgcgct ccaccgagat ggtgaccttc ctcttggcac gtggtgcgga cgtcaaccag
     1201 cgcgaccgcg acggctggac gccgctcatg acggcggcgt acggcgatga tgccgccgac
     1261 gtcaagctgc tcatcgccca cggcgccgat gcgaatgcgg tcagcgcgca gaacttcacg
     1321 ccgctcggta tcgccacgca gtacgacaag agcaaggccg cgctggcgct catcgagggc
     1381 ggcgccgatc cggcccgagc catcggcgat gccggctata caccgctgat gctcgccacc
     1441 gccaatcacg atgcgcaggt ggcgcgcgcg ctcatccaga agggcgcgga cgtcaatgcc
     1501 cacaacagtg gcggcgtcac cgcgctcatg atcgccgccg ccgggagccg cgccgacatg
     1561 gtcgagctgc tggtgcaggc gggtgccaat gtgcaggcac agaccgaacg cggcgagacg
     1621 gcgctatcca tcgcccgcac caagggtgac gagaaggtca ttcgcctgct cgatggggca
     1681 gcggcgcatc cgggagcgta ggccgggacg tgtgcagaag gtgggatgag gtgcgcggtg
     1741 ctcaggcgaa cattgaccat ttctattctc gggctggcga cgatcacggt gctgtacgcg
     1801 caaccgcaga cgccgccgcc cgccgctgcg ccgccgcccg ccgcgggcgc gccggcaggc
     1861 gctgcgggtg ctggcggcgc taccatgacg ccggtgcagc gcgcggacgc cacgccgaag
     1921 gggcagctga aaaatcccta ttccgacagc gatgccacgg tcgtgcaggc gggccagaag
     1981 ctgtacatga actacggttg cagcggctgc cacggtggca ccgggggc
//
LOCUS       DATDMP010000542         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_198125, whole genome shotgun sequence.
ACCESSION   DATDMP010000542 DATDMP010000000
VERSION     DATDMP010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198125"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1727)
                     /locus_tag="VI195_09500"
     CDS             complement(153..1727)
                     /locus_tag="VI195_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6824665.1"
                     /translation="MDKPTPAVWPDTGEGPYFHERVLLAAIVDSSDDAIVSKTLEGKI
                     LSWNRGAARIFGYEAHEAIGQPITIIIPPELHAEEQQILDKLRRGERIEHFDTVRITK
                     DGRRIAISLTVSPVRAPDGTVIAASKVARDVSDRKLAEERLRASQEALRLADRRKDEF
                     LALLAHELRNPLAPIRYALAANKKPGRTPEQCRQAEEIIERQVMHMSRLLDDMLDISR
                     ITRGVLELKRTRAELTSVIGAAIETARPMLDEKQHTLALDLPSEPVQLEADVVRLAQV
                     FSNLLINAAKYTDPGGRIQLRARSQGSEVLVTVSDNGIGIPADLLPRVFNMFFQSHSA
                     LARAEGGLGVGLSLVRGLVRLHGGTVEARSDGSGRGSEFIVRLPLGPPSPQLEDAEVE
                     GEQISADAGLKILVVDDNRDAADTCAMLLEASGHHVQTAYTGRQALELALTFRPHALL
                     LDIGLPDMDGYQLAQQIRAKPWGRTAVLVAVTGWGQEQDRQRAVAAGFDQHLVKPISA
                     ETVETLLHSLARPGPH"
     gene            1907..2254
                     /locus_tag="VI195_09505"
     CDS             1907..2254
                     /locus_tag="VI195_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824666.1"
                     /translation="MELLWRFLLGGALVSSFALIGDVLRPKRFAGIFGGAPSVALATL
                     ALTAAKSGPAIASLEARSLILSSLGFVLYVYVARKLLATTRWSPLLVSLGGLTIWGLV
                     AVTAGLLLRLAGT"
     gene            2251..>2561
                     /locus_tag="VI195_09510"
     CDS             2251..>2561
                     /locus_tag="VI195_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824667.1"
                     /translation="MIPAVNFASLKEARWWQFAVRFFLGGAITVCTGVIAQRFGPVVG
                     GLFLSFPAIFPATATLIERHEREKKSKAGIDGQARGRKAAALDAAGAIFGGWGMLCFG
                     CV"
BASE COUNT          375 a          826 c          861 g          499 t
ORIGIN      
        1 cttggccccc tggtggccca atgcgctgcg taccgatttc ggcaggcgag tgatggtgcg
       61 cttgcgacgt gcggccgctg ccgccttgcg gatgttggcg cgcgccgcgg cgcgttgctt
      121 tgcactgctc atgcggatgc cgcggccgcg cttcagtgag gtcccggacg ggcgagcgag
      181 tgcagcagcg tttccaccgt ctcagccgag atcggtttca ccagatgctg atcgaagcct
      241 gccgccacgg cacgctgtcg gtcctgctcc tgaccccacc cggtgactgc aaccagcacc
      301 gcggtgcgcc cccagggctt ggcgcggatc tgctgggcca gctgatagcc gtccatgtcg
      361 ggcagtccga tgtccagcaa cagtgcgtgc ggccggaagg taagcgccag ctcgagtgcc
      421 tgtctgccgg tgtaggccgt ctgcacatga tggcccgagg cttccagcag catggcgcag
      481 gtatcggccg cgtcccggtt gtcgtcgacc accagaatct tgaggcccgc gtccgcggag
      541 atctgttcgc cctccacctc ggcatcctcc agctgtggcg aaggagggcc caacggcagc
      601 cgcacgatga actcgcttcc ccgtcccgag ccgtcgctgc gggcttcgac agttccgcca
      661 tgcagtcgca cgaggccgcg taccagcgag agacctacac cgagtccgcc ttcggcgcgc
      721 gcgagcgccg agtgcgactg gaagaacatg ttgaacacac gcggcagtaa gtccgccggg
      781 atgccgatac cgttatcgct caccgtgacc agcacttcgc ttccctgcga gcgggcccgc
      841 agctggatgc ggccgcccgg gtcggtgtat ttggcggcat tgatcagcag attggagaac
      901 acctgagcca ggcgcaccac atcggcctcg agctgcaccg gctcgctcgg aaggtcgagg
      961 gcaagggtgt gctgcttctc gtccaacatg ggccgggccg tctcgatcgc ggcaccgatc
     1021 acggaggtga gctccgcacg agtccttttc agctcgagga ctccgcgggt gatgcgcgag
     1081 atatcgagca tgtcatcgag caggcggctc atgtgcatga cctggcgctc gatgatttcc
     1141 tcggcctgtc tgcactgctc gggggtgcgg ccaggtttct tgttcgccgc cagtgcgtag
     1201 cgaatcggcg cgagcgggtt acgcagttcg tgcgccaaca gtgcaaggaa ctcatccttg
     1261 cggcgatcgg cgagccgcaa cgcttcctgg ctggcgcgca gccgctcctc cgccagtttg
     1321 cggtcggaga catcgcgcgc caccttcgaa gctgcgatga cggttccatc gggcgcgcgc
     1381 accggggaca ccgtcagaga aatggcgatg cgcctgccgt ccttggtgat gcgcaccgtg
     1441 tcgaagtgtt cgatgcgctc gccacgcctg agcttgtcga ggatctgctg ctcctccgca
     1501 tgcagctccg gcgggatgat gatcgtgatc ggctggccga tcgcctcgtg cgcctcgtag
     1561 ccgaagatgc gcgcggcgcc gcggttccag gagaggatct ttccctcgag cgtcttgctg
     1621 acgatcgcat cgtccgatga gtcgacgatg gcggcgagca atacgcgctc gtggaaatat
     1681 gggccctcgc cggtatccgg ccacactgcg ggcgtaggct tgtccataag ctcgcggacc
     1741 ttggcccgcg ctcggctgcg atgcgccttg agagatcgat cggcttccac gcgagatcct
     1801 cgctgagtcg gcagagtata aagcacgcgc gctgtcgtgg cggtcaagtc ctacggccca
     1861 gcgtgggccg actgcgtgcg ccggcgtctc tggtaacctc tgacgcatgg agctgctgtg
     1921 gcgatttctc ctcggggggg cgctggtcag ctcgttcgcg ctgattggtg atgtgctgcg
     1981 gcccaagcgc ttcgcgggca ttttcggtgg cgctccatcg gtggcgcttg ccactctggc
     2041 gctcactgcc gcgaagtccg gccccgcaat cgcctcgctc gaagcgcgct cgctgatcct
     2101 cagcagcctc ggtttcgtgc tctacgttta cgtggcacgg aaactcctgg ccaccacccg
     2161 ctggtcgccg ttgttggttt cactcggggg cctcacgatc tggggcctgg tcgccgtgac
     2221 ggcaggcctg ctgctgcgac tcgccggcac atgattccgg cggtcaattt cgccagcctc
     2281 aaggaagcgc gctggtggca gttcgcggtg cggtttttcc tcgggggcgc gattaccgtg
     2341 tgcacaggtg tcatcgcgca gcgcttcggt ccagtcgtcg gcggattgtt tttgtcattt
     2401 ccggcgattt ttcccgcgac cgccacgctg atcgagcggc acgagagaga gaagaaaagc
     2461 aaggccggca tcgatggcca ggcgcgcggc cgcaaggctg cggctctcga tgccgcgggg
     2521 gcgatattcg gtggatgggg aatgctgtgt ttcggctgcg t
//
LOCUS       DATDMP010000543         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1309575, whole genome shotgun sequence.
ACCESSION   DATDMP010000543 DATDMP010000000
VERSION     DATDMP010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309575"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..573)
                     /gene="rplJ"
                     /locus_tag="VI195_09515"
     CDS             complement(40..573)
                     /gene="rplJ"
                     /locus_tag="VI195_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY6824668.1"
                     /translation="MALNLEDKKALVAEVAEVAAHAQSVVAAEYRGLTVSQMTELRAR
                     ARRQGVYMRVVKNTLARKALAGTTFESVGPKLKGPLMLAFSKDDPGAAARVVKDFARG
                     NEKLVATLVSLGGQVLPAAELDKVASLPTREQALSMLLGVLKAPIQKLVGTLAAPASQ
                     LARTIAAVREQKQAAGA"
     gene            complement(821..1507)
                     /gene="rplA"
                     /locus_tag="VI195_09520"
     CDS             complement(821..1507)
                     /gene="rplA"
                     /locus_tag="VI195_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY6824669.1"
                     /translation="MAVAKRIKAWKAKLTPGKSYSVDEAIKLVKEFATAKFAESVDAA
                     VNLGIDASKSDQQVRGSTVMPHGTGKTVRVAVFTSGKNQEAAKAAGADIVGLEELAAK
                     VKAGEINFDRVIASPDAMRVVGQLGQILGPRGLMPNPKVGTVTPDVATAVKNAKAGQV
                     TYRVDKAGIVHCTIGKANFEANALKENLHTLIADLQKAKPASSKGVYLKRVTLSSTMG
                     PGVLVDQATI"
     gene            complement(1509..1940)
                     /gene="rplK"
                     /locus_tag="VI195_09525"
     CDS             complement(1509..1940)
                     /gene="rplK"
                     /locus_tag="VI195_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY6824670.1"
                     /translation="MAKKVQGYIRLQVPAGSANPSPPIGPALGQQGVNIMEFCKQFNA
                     QTQKVEKGLPIPVVITVYSDRSFTFVMKTPPAAVLIRKAIGIEKGSGTPNTAKVGKIS
                     RKQLEEIAKLKQPDLTAADLDAAVRTIAGSARSMGVDVEGV"
     gene            complement(2120..2653)
                     /gene="nusG"
                     /locus_tag="VI195_09530"
     CDS             complement(2120..2653)
                     /gene="nusG"
                     /locus_tag="VI195_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY6824671.1"
                     /translation="MGLRWYVVHAYSNFEHRVAESLKERIQRAGLAERFGEILVPTEE
                     VVEMRDGQKRKSERKFYPGYVLVQMEMDEETWHLVRDVPKVLGFIGGTPERPAAISDK
                     EANQILRRVEEGVDKPRPKVLFEPGEVVRVVDGPFNDFNGVVESVNYEKNRLQVAVQI
                     LGRSTPVELDFSQVEKA"
     gene            complement(2656..3036)
                     /gene="secE"
                     /locus_tag="VI195_09535"
     CDS             complement(2656..3036)
                     /gene="secE"
                     /locus_tag="VI195_09535"
                     /inference="COORDINATES: protein motif:HMM:NF004371.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY6824672.1"
                     /translation="MSDDIKVQDAGTADKLKLAVAIVIVIAGVAAYYVLADQSVWMRW
                     LPVIGSLALAAVVVAFSRYGSEFRRFVELARIELRKIVWPTRQETLQTTLVVFGFVIV
                     AGIFFWLLDLVLAWATKTLTGTGG"
     gene            complement(3047..3122)
                     /locus_tag="VI195_09540"
     tRNA            complement(3047..3122)
                     /locus_tag="VI195_09540"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3087..3089),aa:Trp,seq:cca)
BASE COUNT          526 a         1121 c          941 g          608 t
ORIGIN      
        1 acagccatgt gagtctccaa aaagtttgtg atcgaaccgt caggcgcccg ccgcctgctt
       61 ctgctcacgc accgcggcga tggtgcgcgc caactgcgat gccggcgccg cgagcgtgcc
      121 gacgagcttc tggatcggcg ccttgagcac gcccagcagc atcgacaacg cctgctcgcg
      181 cgtcggcagg ctcgcgacct tgtcgagctc cgctgcgggc agcacctgcc cgccgagaga
      241 cacgagcgtc gccacgagct tctcgttccc cctcgcgaag tccttcacca cgcgcgccgc
      301 cgcgcctgga tcgtccttcg agaaggcgag catcaatggc cccttgagct tcggtcccac
      361 agattcgaac gtggtgccgg cgagagcctt gcgcgccagc gtgttcttta ctacacgcat
      421 gtacacgcct tggcgccgcg cccgggcacg cagctcggtc atctgggaga ccgtgaggcc
      481 acggtactcg gcggctacga ccgactgggc atgcgctgcc acctcggcca cctccgccac
      541 gagcgccttt ttgtcttcca ggttgagtgc cagtcatctc tcctcttaac gtggacggtg
      601 acctccacgc ctcgaggagc atcctcgatg cgcatagcgg ttcaccatct gcgcaggcgg
      661 ctcattaaga gggccggggt ttggccatgc ctcaccttcc ctcgcctgcg gtcttcgatg
      721 atgaccggag tcatcggcac tccggccttc ccgcgcgtca ccacgcggga gttacgcggt
      781 tcgcgccaga gcgcacaccg cgttcatcaa aaccaaaaac ctaaatcgtc gcctggtcca
      841 cgagcacacc cggccccatg gtcgaggaca gggtgacccg cttcagatac acacccttcg
      901 aggaggcggg cttcgccttc tgcaggtccg cgatcaacgt gtgaaggttc tccttcagcg
      961 cgttcgcctc gaagttggcc ttgccgatcg tgcaatgcac gatgccggcc ttgtcgactc
     1021 gataggtcac ctgcccggcc ttcgcgttct tcaccgcggt cgcgacatcc ggggtcacgg
     1081 tgcccacttt ggggttcggc atcagcccgc gcggaccgag gatctgcccg agctgcccga
     1141 ccacacgcat ggcgtcgggg ctcgcgatga cgcggtcgaa gttgatctcg cccgccttca
     1201 ccttcgccgc cagctcctcc aatccaacga tgtcggcacc cgccgccttg gccgcctcct
     1261 ggttcttgcc ggaggtgaac acggcgacgc gcaccgtctt gcccgtgccg tgcggcatca
     1321 cggtcgagcc acgcacctgc tggtcggact tgctcgcgtc gatgccgaga ttcacggccg
     1381 catccacgct ctcggcgaac ttggcggtcg cgaactcctt gacgagcttg atggcctcat
     1441 cgaccgaata cgacttgccg ggagtcagct tggccttcca ggccttgata cgcttggcga
     1501 cggccatatc acaccccttc cacatcgacg cccatgctgc gggcactgcc cgcgatggtg
     1561 cgcaccgcgg cgtcgaggtc cgcggcggtg aggtcgggct gcttgagctt ggcgatctct
     1621 tccagctgct tgcgcgagat cttgccaacc ttcgcggtat tgggggtgcc gctgcctttt
     1681 tcaatgccga tcgccttgcg gatgaggacc gccgccgggg gggtcttcat cacgaaggtg
     1741 aagctgcgat cggaatagac ggtgatcacc accgggatcg gcaggccctt ctccaccttc
     1801 tgggtctgcg cattgaactg cttgcagaac tccatgatgt tcacgccctg ctgaccgagc
     1861 gccggcccga tcggcggcga ggggttggct gagcctgccg gcacctgcag cctgatgtag
     1921 ccctgtactt tcttagccat cgattacctg acctccaggt cccagcgcct tgcggctccc
     1981 tgttaaggat gtcggttgca aacacgcggc ctgcgccgcg acccttgtcc atggggcaaa
     2041 atcgcctgcg gcgattttgc cggacatcgc tccgtcggcc gccggcaaaa ggcgtggttt
     2101 tcgccggctg cgcctcaatt catgccttct cgacctgcga gaaatccagc tccaccggtg
     2161 tcgagcgtcc gaggatctgc accgccacct gcaggcggtt cttctcgtag ttgacgcttt
     2221 cgaccacgcc gttgaagtcg ttgaacggcc catcgaccac gcgcaccacc tcgccaggct
     2281 cgaacagcac cttcggccgc ggcttgtcga cgccttcctc gacgcggcgc aggatctggt
     2341 tcgcctcctt gtcgctgatg gcggccggtc tctccggggt gccgccgatg aagccgagca
     2401 ccttcggcac atcacgcacc agatgccagg tctcctcgtc catctccatc tgcaccagca
     2461 cgtagccggg gtagaacttg cgctcgctct tgcgcttctg gccgtcacgc atctcgacga
     2521 cttcctccgt cggcaccagg atctcgccga agcgctcggc cagcccggcg cgctggatgc
     2581 gctccttgag cgactcggcg acgcgatgct cgaaattcga gtaggcgtgc acgacgtacc
     2641 atctcaaccc cacgttcagc ctcccgtccc ggtaagggtc ttcgtggccc aggcgagcac
     2701 gagatccagc agccagaaga aaattcccgc gacgatcacg aagccgaaca ccacgagcgt
     2761 cgtctgcagt gtctcctggc gcgtcggcca gacgatcttg cgcagctcga tgcgcgcgag
     2821 ctctacgaag cggcggaact cgctgccata gcgggagaaa gccaccacca ccgcggcgag
     2881 cgcgaggctc ccgatcaccg gcagccagcg catccagaca ctctgatccg cgagcacgta
     2941 ataagcggcc acgccggcga tcacgatcac gatcgcgaca gcgagcttca gcttgtccgc
     3001 cgtgccggcg tcctgcactt tgatgtcgtc actcataaca aatcgctggc aggccaggag
     3061 ggaatcgaac ccccaacctg cggttttgga gaccgccgct ctgccaattg agctactggc
     3121 ctgtgttgat tggccgaaat cgctcccagc gatttcggcg gacatcgctc cgtcgaccgt
     3181 gggcaaaaag cgtggt
//
LOCUS       DATDMP010000544         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1530756, whole genome shotgun sequence.
ACCESSION   DATDMP010000544 DATDMP010000000
VERSION     DATDMP010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530756"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VI195_09545"
     CDS             complement(<1..634)
                     /locus_tag="VI195_09545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824673.1"
                     /translation="MRVRWKIFLYLFGFGFVAYLQQRGLTVASYDMMPNLHLNQMQIG
                     WLLQSMLLGYTVLQFPGGVIGQRVGARWMFLIISLIAFAATLGTPLVPLFLTGTALFG
                     ALLAAQLLLGASQGPIFPVSAGVFEAWFPSRQWPLVQGLQSMGLQLGAALTPPLIASL
                     MVSFGWQRALAWSTLPVLALIGGWAWYGRNTPEEHPRVSAAELAELDPPSG"
     gene            complement(679..1836)
                     /locus_tag="VI195_09550"
     CDS             complement(679..1836)
                     /locus_tag="VI195_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY6824674.1"
                     /translation="MSAPERPSRPALEPALPSSWYRSERVWAAEKERIFCREWLCVGR
                     GEELADEPGAYHLLDVLGESIILVRNRERALRAFYNTCRHRGSRLCREMASTGAVPHL
                     TGGLRAGRITCPYHQWSYDLDGRLLGAPHMAGVAGFDKAQFGLYPVGVAVWGGFIFLN
                     LTPSSALPLPQQLGPVPARIERYPLAELRIGHSIRYEVAANWKVLCENYNECYHCAGV
                     HPELCEVVPAFGERGGAGLDWLRGIPHRTGAYTFTRSGTTHRRPFPTLTEEERVRHKG
                     ELVYPNLFLSLACDHVAAFVLTPRGPAATSIDCHFLFEPYEIAQPGFDPLDTVEFWDL
                     VNRQDWAICEAVQQGISARVHERGYYAPMEDYNLDIRRYVLERIGDAVDGA"
     gene            complement(1833..>2180)
                     /locus_tag="VI195_09555"
     CDS             complement(1833..>2180)
                     /locus_tag="VI195_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824675.1"
                     /translation="ESAGIALHTLSRVSAFAGGSVTLANQFTNSETLLPCASLVIVGT
                     RFANDALHAELMAREAEFADAAIRSVTRIGDALAPGAIVHAVYSGRRYAEEFDADPRA
                     LPYRRDAPLARQA"
BASE COUNT          397 a          725 c          751 g          307 t
ORIGIN      
        1 ctccgcttgg ggggtcgagc tcggcgagct cggcggcact gacccgcgga tgctcttccg
       61 gtgtgttacg gccgtaccac gcccacccgc cgatcagggc gagtaccggc aacgtgctcc
      121 aggcgagcgc acgctgccag ccgaaactca ccatgagcga ggcgatcagc ggcggggtga
      181 gcgccgcgcc gagctgcaga cccatgctct gcaaaccctg cacgagcggc cactgccgcg
      241 agggaaacca cgcctcgaac actccggcgg agacgggaaa gatcggtccc tgcgacgcgc
      301 cgagcagcag ctgcgcggcc agcagcgcgc caaagagcgc cgtaccggtg aggaagagcg
      361 gcacgagcgg agtgccgagc gtcgcggcga aggcgatgag gctgatgatc aggaacatcc
      421 agcgggcgcc gacgcgctgg ccgatgacgc caccgggaaa ctgcaagacc gtgtaaccga
      481 gcagcatcga ctgcaacagc cagccgatct gcatctggtt caagtgtagg ttcggcatca
      541 tgtcgtagct cgcgaccgtg agaccgcgct gctgcaggta cgcgacgaag ccgaagccga
      601 aaagatagag aaagatcttc cagcgcacac gcacgcgcgg cggagagcgg ggcggggatg
      661 tggcgtgcgg ctcgccgctc acgcgccatc gaccgcgtcg cctatccgct cgaggacgta
      721 gcgacggatg tcgaggttgt agtcttccat cggtgcatag tagccgcgct cgtggacgcg
      781 cgcgctgatt ccctgttgca ccgcctcgca aatcgcccag tcctggcggt tgacgagatc
      841 ccagaactcg accgtatcga gcggatcgaa gcccggctgt gcgatctcgt agggctcgaa
      901 caggaaatga cagtcgatac tcgtcgcggc cggcccgcgc ggcgtgagaa cgaacgcggc
      961 gacgtggtca caggccaggc tcaagaacaa attgggatag acgagctcgc ccttgtggcg
     1021 cacgcgctct tcctcggtca gtgtcggaaa cggccggcgg tgcgtcgtgc cggagcgggt
     1081 aaaggtgtag gcgccggtgc gatgcggtat gccgcgcagc cagtcgagtc ccgcaccgcc
     1141 gcgctcgccg aaggccggca ccacttcgca cagttctggg tgcacacctg cgcagtggta
     1201 gcactcgttg tagttctcgc acagcacctt ccagttcgcc gcgacctcgt agcggatcga
     1261 gtgaccgatg cgcagctccg cgagcgggta gcgttcaata cgcgcgggga ccgggccgag
     1321 ctgctgcggc agcggcagcg cggacgatgg cgtcaggtta aggaagatga agccgcccca
     1381 cactgcgacc ccgaccggat aaagcccaaa ctgcgccttg tcgaagcccg caacgcccgc
     1441 catgtgcggg gcgccgagca gccggccatc gagatcgtag ctccactggt ggtaggggca
     1501 ggtgatgcgc ccagcgcgca ggcccccggt gagatgcggt acggcacccg tgctggccat
     1561 ctcacggcac aaacgcgagc cgcggtgccg gcaagtgttg tagaaggccc gcaacgcgcg
     1621 ctcgcgattg cgcaccagga tgatgctttc gccgagcaca tcgaggagat gataggcgcc
     1681 gggctcatcg gcgagctctt ccccccgccc gacgcatagc cactcgcggc agaagatgcg
     1741 ctctttctcc gccgcccaca cacgctccga gcggtaccag ctcgagggca gcgccggctc
     1801 gagcgccggg cgcgatggcc tctcgggcgc gctcacgcct gcctcgctag cggtgcatcc
     1861 cgccggtaag gcagcgcccg cgggtcggca tcgaattcct cggcgtagcg gcggccgctg
     1921 tagacggcgt gcacgatcgc accgggggcg agtgcatcgc cgatgcgcgt caccgagcga
     1981 atcgccgcat ccgcgaactc cgcttcccgc gccatgagct cggcatggag cgcatcgttc
     2041 gcgaaacggg tgccgacgat cacgagcgag gcgcagggga gcagagtctc gctgttggtg
     2101 aactggttgg cgagcgtgac gctgccaccc gcgaacgcgc tcacccgcga gagcgtgtgc
     2161 agcgcgatcc cggccgactc
//
LOCUS       DATDMP010000545         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1530819, whole genome shotgun sequence.
ACCESSION   DATDMP010000545 DATDMP010000000
VERSION     DATDMP010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530819"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VI195_09560"
     CDS             complement(<1..386)
                     /locus_tag="VI195_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6824676.1"
                     /translation="MKIRVLGSAAGGGFPQWNCNCRNCAGVRAGTLRALRRTQSSIAV
                     RGREPDSWVLVNASPDILGQLAAYPDLQPARAPRDTALAAIVLVDGQVDHTTGLFMLR
                     ESTRPLSVWCTPAACADLTRGNPIFAV"
     gene            703..2049
                     /locus_tag="VI195_09565"
     CDS             703..2049
                     /locus_tag="VI195_09565"
                     /inference="COORDINATES: protein motif:HMM:NF027763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824677.1"
                     /translation="MKLRTRLYLVLTGVTTVFVAVLIADEVRDARSSVREEIEAANRV
                     AAHVLQSLIITYAASGGTPAVQTMLEQLGHVRANDLTLRDASGKVLYHSPPPAYKAGR
                     EAPAWFTHLLAPRPAQQVFTLGDGAQVVITAQPSRAILDAWDDLARLLAIAATMIAAL
                     GGVAFWLVNRALAPFPVIADGLERLQRGELGFRLPPLRGYEAHAIGAAFNRMAQAMED
                     KVRAEREAHEARARLEEGRELAQLVEQSVEEERHLIAHELHDEFGQSVTAIRSLAMAI
                     ATQSGEPGMSETARLISDEAARLYDAMHGLIPRLAPPSLDTLGLAESLETLVGDWQQR
                     HASPRLALRHQLPADLGTSVTLAAYRVVQEGLVNAVRHARAQHVAIEVGVADDRMIVS
                     VSDDGVGLPENWSRPGHFGLRGLSERVRRLGGSLQVVNGQPRGACLRAEIPLAARA"
     gene            2046..>2423
                     /locus_tag="VI195_09570"
     CDS             2046..>2423
                     /locus_tag="VI195_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6824678.1"
                     /translation="MIRVLLVDDHAVVRTGFRLLLQAHPEITVVGEADSGELAYQRYL
                     ELTPDVVVMDLAMPGMGGLEALRRIRAHHPQARVLTLSAHDDPLHAQRALREGARGFL
                     SKRSAPEALLEAITAVAAGQRYLD"
BASE COUNT          379 a          811 c          844 g          389 t
ORIGIN      
        1 acggcgaaaa tcgggttgcc gcgcgtcagg tccgcacagg cggcgggcgt gcaccacacc
       61 gacaacggcc gtgtggattc tctcagcatg aaaagaccgg tcgtatgatc gacctgccca
      121 tcgaccagca cgatcgcggc cagggcggtg tcgcgcggcg cgcgcgccgg ctgaaggtcg
      181 ggatacgccg ccagctgccc gagaatgtcg ggcgaggcat tgacgagtac ccaggagtcg
      241 ggctcgcgac cgcgcacggc gatcgatgat tgcgtgcgcc gcagcgcccg caacgtgccc
      301 gcgcgcacgc ctgcgcagtt acgacagtta cagttccact ggggaaaacc gccgcctgcg
      361 gccgatccca gcacgcggat cttcacggct tcatcgggca ggggtcaggg cccgctcccg
      421 gtgtgcgtgc ggagcccgag cggcccaggc tcccggccta ttggttcaga attagcgtcg
      481 ttcagcggtt cgcaatgtac atcgtgattt ccataccgaa ccgaaaatca atggcttgtg
      541 gtgtttccca gcgcatagtc tatgttcctg gttggttccg ggtgggatcg aacgctcgac
      601 gtgtatcctt ggccggacga ggctggatgc gaaagcagat gatcatgaaa gcggcctcat
      661 gattttcatg aggacacccg gctcgagccg cacgagccgc cgatgaagct gcgcacccgg
      721 ctctacctcg tgctgacggg ggtcacgacc gtcttcgtgg ccgtgctcat cgccgatgag
      781 gttcgcgacg cgcgctcctc ggtgagagag gagatcgaag cggccaatcg cgtggccgct
      841 cacgtgctgc agagtctcat catcacctat gccgccagcg gcggcactcc ggccgtgcaa
      901 acgatgctcg agcaactcgg ccacgtccgc gccaacgacc tcacgctgcg cgatgcctcc
      961 ggcaaggtgc tctaccattc ccctccaccg gcctacaagg cggggcggga ggcgccggca
     1021 tggttcactc atctgctggc accgaggccg gcgcagcagg tgttcacgct gggtgacggc
     1081 gcgcaggtgg tgatcaccgc acaaccctcg cgcgccatac tcgatgcctg ggacgatctg
     1141 gcacggctgc tcgcgatcgc cgccaccatg atcgccgcgc tcggcggagt tgccttctgg
     1201 ctcgtgaacc gcgcgcttgc gccctttccg gtgatcgccg atggcctcga gcgcctgcag
     1261 cgcggcgagc tcggcttccg cctcccgccc ttgcgcggtt acgaggcgca tgcaatcggt
     1321 gcggctttca atcgcatggc ccaggcgatg gaagacaagg tgcgcgcgga gcgcgaggcg
     1381 cacgaggcgc gcgcgcggct cgaagagggc cgcgagctcg cgcagctggt ggagcaaagc
     1441 gtggaggagg agcggcacct gatcgcgcac gagctgcacg atgagttcgg ccagtcggtg
     1501 accgcgatac gcagcctcgc catggcgatc gcgacgcagt ccggcgagcc gggcatgagc
     1561 gagacggcgc gcctgatctc ggatgaggcg gcgcggctgt acgacgccat gcacggcctg
     1621 atcccgcgcc ttgcgccgcc atcgctcgat accctgggac tggcggagtc gctcgaaacc
     1681 ctggtgggcg actggcagca gcgccatgct tcgccccgcc tcgcgctgcg gcatcagctg
     1741 ccggctgacc tcggcaccag tgtgacgctc gcggcctacc gcgtggtgca ggaaggcctg
     1801 gtgaacgcgg tgcgccacgc ccgcgcgcag cacgtcgcga tcgaggtcgg tgtcgcggac
     1861 gatcgcatga tcgtctcggt gagcgatgac ggcgtcggtc tgccggagaa ctggtcgcgg
     1921 cccggtcact tcggcctgcg cggcctcagc gagcgagtga ggcgcctggg cgggagcctg
     1981 caggtggtca acggccagcc acgcggggcc tgtctgcgcg cggaaattcc cctggcagcg
     2041 cgcgcatgat ccgcgtgctg ctggtcgacg atcacgccgt ggtgcgcacc ggctttcgat
     2101 tgctgctgca ggcgcatccg gagatcaccg tcgtgggcga ggcagactcc ggcgagctcg
     2161 cgtaccagcg ctatctcgag ctgacaccgg atgtcgtggt catggatctt gccatgcccg
     2221 gcatgggcgg cctcgaggcg ctgcggcgca tccgcgcgca ccatccgcag gcgcgcgtgc
     2281 tcacgctgtc cgcgcacgac gacccgctgc acgcgcagcg tgcgctgcgt gagggggcgc
     2341 gcgggtttct ctccaaacgc agtgcgcccg aagcgctgct cgaggcgatc accgcggtgg
     2401 cggcggggca gcgctatctc gat
//
LOCUS       DATDMP010000546         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1197632, whole genome shotgun sequence.
ACCESSION   DATDMP010000546 DATDMP010000000
VERSION     DATDMP010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197632"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VI195_09575"
     CDS             complement(<1..905)
                     /locus_tag="VI195_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6824679.1"
                     /translation="MRFLFKTDYEQDIRLFKHGGQRFWYGLLALLLLAAPYLAGEYLM
                     SQLQFICIYSIVGLGLMLLVGFTGQISLGHAAFLAIGAYTEALLQAHGVPFVVSLPAA
                     ALLSAAFGWVVGLPALRLKGIYLAIATLAFNVIIEEAITRWEGLTNGNSGLHLKPIEL
                     AGLKLDGDASFYYLCLALTGLAVLALLNLLRSPTGRAFVAIRDSEISASCMGVNLATY
                     KTMSFALSAALTGVAGALYAHKVTFISPEQFTLLQSIELVTIVILGGVGFVHGAILGA
                     AFLIVLPQLISLAKDYLPADVGGTGL"
     gene            complement(906..1775)
                     /locus_tag="VI195_09580"
     CDS             complement(906..1775)
                     /locus_tag="VI195_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6824680.1"
                     /translation="MLQLLLAGVAQGCVYALVALGFVLIYKATETVNFAQGDLMMLGG
                     FFALTASVILGWPYWATILFAVAAMAAVGMLIERLVLRPVLGQPAFTVVMITIGVGYV
                     LRGAVTMVPGWGSDTYTFATPFADATFRLGEVIFAVQQVAVIVMTATLVAVLYVFFRF
                     SKLGVAMQATSQNQLAAAYMGIPVRRVNMMIWGLSAGVAAFAGILLAPVTFVHSNMGF
                     IGLKAFPAAVVGGFGSVPGAIVGGLIIGVVEAFAGRYLPEGFKDVAAYVVVLAVLLVR
                     PSGIFGETARKKV"
     gene            1823..>2277
                     /locus_tag="VI195_09585"
     CDS             1823..>2277
                     /locus_tag="VI195_09585"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY6824681.1"
                     /translation="MKTVRVGAGLGFYGDSWRPVRASIERGGVQYIASDHLSELTLAI
                     LQKDRQRDRPGDAPSGYARDAVPMLAELWPLAAKNKVRFVLNAGGLNPEGAREAIART
                     FHDKGWKAKIATVTGDSVLERVDELRAAGEALAHMDTGADIVSVRDRML"
BASE COUNT          445 a          756 c          781 g          295 t
ORIGIN      
        1 aggccggtgc cgcccacgtc ggcgggcagg tagtccttgg ccagcgagat gagctgcggc
       61 agcacgatga ggaacgccgc cccgaggatc gcgccgtgca cgaagccgac gccgccgagg
      121 atcacgatgg tgaccagctc gatcgactgc agcagcgtga actgctcggg cgagatgaag
      181 gtcaccttgt gcgcgtacag cgcgccggcg accccggtga gcgccgccga gagcgcgaac
      241 gacatcgtct tgtaggtggc gaggttgacg cccatgcagg aggccgagat ctcggagtcg
      301 cggatcgcga cgaaggcgcg ccccgtgggc gagcgcagca ggttcagcag cgcgagcacc
      361 gcgagcccgg tgagcgcgag gcacaggtag tagaagctcg cgtcgccgtc cagcttcagg
      421 cccgccagct cgatcggctt caggtgcagg ccggagttgc cgttggtcag gccctcccag
      481 cgcgtgatcg cctcctcgat gatcacgttg aaggcgagcg tggcgatcgc gaggtagatg
      541 cccttgaggc gcagcgcggg cagcccgacc acccagccga acgccgccga cagcagcgcc
      601 gcggccggca gcgagaccac gaaggggacg ccatgcgcct gcagcagcgc ctcggtatac
      661 gcgccgatgg cgaggaaggc cgcgtggccg agcgaaatct gcccggtgaa gcccaccagc
      721 agcatcaggc ccaggccgac gatggagtag atgcagatga actgcagctg cgacatcagg
      781 tattcgccgg ccaggtacgg cgcggcgagc agcagcagcg cgagcaggcc gtaccagaag
      841 cgctggccgc cgtgcttgaa caggcgaatg tcctgctcgt aatcggtctt gaacaggaat
      901 cgcattcaga ccttctttcg cgccgtttcg ccgaaaatgc cggaggggcg gacgagcaac
      961 accgccagca ccaccacata ggcggccacg tccttgaaac cctcgggcag gtagcgcccg
     1021 gcgaaagcct cgaccacgcc gatgatgagg ccgccgacga tcgcgcccgg cacgctgccg
     1081 aagccgccca ccaccgccgc cggaaaggcc ttgaggccga tgaagcccat gttcgaatgt
     1141 acgaaggtca cgggcgcgag caggatgccg gcgaacgcgg cgacgcccgc cgacagtccc
     1201 cagatcatca tgttgacgcg ccgtaccggg atgcccatat aggcggcggc gagctggttc
     1261 tgcgacgtgg cctgcatcgc cacgccgagc ttggaaaagc gaaagaacac gtacagcacc
     1321 gcgaccagcg tcgcggtcat gacgatcacc gccacctgct gcaccgcgaa gatcacctcg
     1381 ccgagcctga aggtggcgtc cgcgaacggc gtggcgaaag tataggtatc gctgccccag
     1441 cccggcacca tcgtgaccgc gccgcgcagc acgtagccga cgccgatggt gatcatcacc
     1501 acggtgaatg cgggctgccc gagcaccggc cgcagcacga ggcgctcgat gagcatgccg
     1561 accgcggcca tcgccgccac cgcgaacagg atcgtcgccc agtacggcca gcccaggatc
     1621 acgctcgcgg taagcgcgaa gaagccgccc agcatcatga gatcgccctg ggcgaagttc
     1681 accgtctcgg tggccttgta gatgagcacg aagccgagcg cgacgagcgc atagacgcag
     1741 ccctgcgcga cgccggcgag cagcagttgc agcacgtgac tcagcggaac tcctcctgga
     1801 aagaattgta gactagcccg caatgaaaac cgtccgtgtc ggcgcgggcc tgggcttcta
     1861 cggcgattcc tggcggccgg tgcgcgcctc gatcgagcgc ggcggcgtgc agtacatcgc
     1921 ctccgatcat ctctcggagc tcacgctcgc gatcctgcag aaggaccggc aaagggacag
     1981 acccggcgac gcgccttccg gctacgctcg cgacgcagta ccgatgctgg ccgagctctg
     2041 gccgctcgcg gcaaaaaaca aggtgcgctt cgtgctgaac gccggcgggc tgaatcccga
     2101 aggtgcgcgc gaggccatcg cccggacgtt ccacgacaag ggctggaagg cgaagatcgc
     2161 cacggtgacg ggcgattcgg tgctcgagcg cgtcgacgag ctgcgcgcgg cgggagaggc
     2221 gcttgcgcat atggacacgg gcgcggacat cgtgagcgtg cgcgaccgca tgctgtt
//
LOCUS       DATDMP010000547         3347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_643407, whole genome shotgun sequence.
ACCESSION   DATDMP010000547 DATDMP010000000
VERSION     DATDMP010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643407"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2482)
                     /gene="fdnG"
                     /locus_tag="VI195_09590"
     CDS             complement(<1..2482)
                     /gene="fdnG"
                     /locus_tag="VI195_09590"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1892..1894),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEY6824682.1"
                     /translation="MADVTRREFLKVTGATLAGSSLVLLGFSPSAALAEVREFKLARA
                     TETRNTCPYCSVACGVLMYSIGDRAKNARSSIFHIEGDPDHPVNRGTLCPKGAALIDF
                     IHSPSRLAHPEYRAAGSDHWQRISWDDALERIAKLMKQDRDANFVAKTPEGLTVNRWL
                     TTGMLAASASSNEVGYLTHKVIRSLGILAFDNQARVUHGPTVAGLAPTFGRGAMTNHW
                     VDIKNADIVLIMGGNAAEAHPCGFKWVTEAKAHNKARLIVVDPRFTRSASVADLYVPI
                     RTGTDIAFLGAVLNYLLSEDKIQHEYVKNYTDMSFIVREDFAFADGLYSGYNADKHSY
                     DKASWDYEIGADGYVKVDPELKHPRCVYQLMKQHYARYTPEMVERVCGTPKEKFLQVC
                     EMIGSTSTPTRAMTVMYALGWTQHSIGSQMIRAGAMMQLLLGNIGVSGGGMNALRGHS
                     NIQGLTDLGLMSNLLPGYLTLPNEKEQEYDKYLAARAYKPLRPNQLSYWQNYNKFYVS
                     LMKAWWGDAASAENHWGYEYLPKLDKGYDMLQGFELMNQGKMNGYICQGFNPLAAAPN
                     KAKMNDALAKLKFLVIMDPLATETSEFWRNFGEHNPVDSAKINTEVFRLPTTCFAEED
                     GALVNSGRWLQWHWKGAEPPGEARSDLQIMGELFTRLRALYKSEGGAYPEPIVNLSWP
                     YVVAQSPSPDELAKEYSGRALKDLIDPKNPTQVTRKAGEQLAGFAELRDDGSTLSGCW
                     IYCGAWGPNGNLMARRDSSDPTGIGQTLNWAFAWPANRRVMYNRASCDTSGKPFNPSR
                     KLIAWNGTAWGGADVPDYKLDEDPSLGMG"
     gene            2684..2757
                     /locus_tag="VI195_09595"
     tRNA            2684..2757
                     /locus_tag="VI195_09595"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2716..2718,aa:Cys,seq:gca)
     gene            2862..>3347
                     /locus_tag="VI195_09600"
     CDS             2862..>3347
                     /locus_tag="VI195_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6824683.1"
                     /translation="MSAKSLMLLGAVFCLPPGSNAAHAAPSDPAAIQVQTLTDALLKS
                     MRAGSTESMAERYRALEPVIERVFALPMVTRLAVGPDWGSFSADDQKAVMAAFTRFTV
                     ANYAHNFHDFDGQRFEIDDNVMTRGPDKIVRSRILPAHDVPVSLLYRMHEVDGAWKII
                     DV"
BASE COUNT          574 a         1033 c         1075 g          665 t
ORIGIN      
        1 gacccatgcc gagcgaaggg tcctcatcga gcttgtagtc tggcacatcg gcccctcccc
       61 aggcggtgcc attccaggcg atcagcttgc gcgaggggtt gaagggcttg ccgctcgtat
      121 cgcaggaggc gcggttgtac atcacgcgcc ggttcgcggg ccacgcgaag gcccagttca
      181 gggtctggcc gatgccggta ggatccgagc tgtcgcgccg cgccatgagg ttgccgttcg
      241 ggccccatgc gccacagtaa atccagcagc cggaaagcgt gctgccgtcg tcccgcagct
      301 ccgcgaagcc ggcgagctgc tcgccggcct tgcgcgtcac ctgtgtgggg tttttcggat
      361 cgataaggtc cttcagtgcg cgtcccgaat attccttcgc gagctcatcc ggactcgggc
      421 tctgcgccac gacatagggc caggagaggt tcacgatcgg ttcgggatag gcccctccct
      481 cgctcttgta gagtgcgcgc agccgcgtga atagctcgcc catgatctgc agatcggagc
      541 gcgcctcccc aggcggctcc gcgcctttcc agtgccactg gagccaccgg cccgaattga
      601 cgagcgcgcc gtcctcctcg gcgaaacagg tggtcggcag gcggaagacc tcggtgttga
      661 tcttggccga atcgaccggg ttgtgctcgc cgaagttgcg ccagaactcg ctggtctcgg
      721 tggcgagcgg gtccatgatc accaggaatt tgagcttggc gagcgcgtca ttcatctttg
      781 ccttgttcgg cgccgccgcg agcggattga aaccctggca gatgtagccg ttcatcttgc
      841 cctggttcat cagctcgaac ccctgcagca tgtcgtagcc cttgtcgagc ttcggcaggt
      901 actcgtaccc ccagtggttc tcggcgctgg ccgcatcccc ccaccaggct ttcatcaggc
      961 tgacatagaa cttgttgtag ttctgccagt agctcagttg gttgggtcgg agcggcttat
     1021 aggcacgtgc ggcgaggtac ttgtcgtact cctgctcctt ctcgttaggc aaagtgagat
     1081 aacccggcag gaggttcgac atcagcccca gatcggtcag cccctggatg ttggagtggc
     1141 cgcggagcgc gttcatgccg ccgccggaga cgccgatatt gccgagcagc agctgcatca
     1201 tggcgcccgc tcggatcatc tgcgagccga tcgagtgctg tgtccagccg agcgcgtaca
     1261 tcacggtcat ggcgcgcgtc ggggtcgaag tcgagccaat catctcgcac acctgcagga
     1321 acttctcctt cggggtgccg cagacgcgct cgaccatctc gggcgtgtag cgcgcgtagt
     1381 gctgcttcat cagctgatac acgcagcgcg gatgcttgag ctcggggtcg accttgacat
     1441 agccatcggc accgatctcg tagtcccagc tcgccttgtc gtagctgtgc ttgtcggcgt
     1501 tgtagcccga atacagccca tcggcgaagg cgaaatcctc gcgcacgatg aagctcatgt
     1561 cggtgtaatt cttgacgtac tcgtgctgga tcttgtcctc gctcagcagg tagttcaaca
     1621 ccgcgccgag gaaggcgatg tcggtgccgg tgcggatcgg cacgtagagg tcggcaaccg
     1681 aggcggagcg cgtgaagcgc ggatcgacga cgatcaggcg cgccttgttg tgtgccttgg
     1741 cctcagtgac ccacttgaag ccgcaggggt gcgcttcggc ggcgttgcca cccatgatga
     1801 gaacgatgtc ggcattcttg atgtcgaccc agtgattcgt catcgctcca cggccaaacg
     1861 tcggggcaag acctgccacc gtcgggccgt gtcacacacg cgcctgattg tcgaaagcta
     1921 ggatgccgag gctgcggatg accttgtgcg tgagatagcc gacttcgttg ctgctcgccg
     1981 acgctgccag catgccggtt gtcagccagc ggttgacggt aagcccctcg ggggtcttcg
     2041 caacgaagtt ggcatcgcga tcctgcttca tgagcttggc gatgcgctcg agcgcgtcgt
     2101 cccaggagat ccgctgccag tgatcggagc cggcagcgcg gtactcagga tgcgccagac
     2161 ggcttgggct gtgaatgaaa tcgatgagcg ccgcaccctt gggacagagc gtgccgcggt
     2221 tcaccgggtg atccgggtcg ccctcgatgt ggaagatgct cgagcgcgcg ttcttggctc
     2281 gatcgccgat gctgtacatc agtacgccgc aggcgaccga acagtaggga caggtgttgc
     2341 gcgtctcggt ggcacgtgcc agcttgaatt cgcgtacctc ggcaagcgca gcgcttggtg
     2401 agaagcccag cagaaccaga ctcgatcccg cgagcgttgc gccagtcact ttgaggaact
     2461 cgcgccgcgt cacatcagcc atggctatcc ccctcggagc atgcctgaag acggcacgga
     2521 ctatactcct ttgagaagaa gcgacaagga cgacgggtgt tccggaccag ccggcaatcg
     2581 acgcccgtag ggccatttgt taggctaccg gttccgcgat tgagagcaca tctcgccgca
     2641 agcagtggtt gtggctcgtt gttccatcgt gatctccacc gttggctagg tggcagagtg
     2701 gtcatgcagc ggcctgcaaa gccgtctacg ccggttcgat tccgacccta gcctccatcg
     2761 gctgaccgtt cgcggctgat atcgtcctgc ctcacgcctg cctgcgctgg ctgcaggccg
     2821 cgagcacggc aggcatcatg taaggcatga gagccatgtg cgtgagcgcg aaatccctga
     2881 tgctcctggg cgcggtgttt tgcctgccgc ctggcagcaa cgcagcgcat gcggccccca
     2941 gcgatcccgc cgcgattcag gtacagacac tcaccgatgc gctgctcaag tccatgcggg
     3001 ccggatcaac tgagtccatg gccgagcgct atcgagctct cgagccggtg atcgaacggg
     3061 tgttcgcatt acccatggtg acccggctgg cggtgggccc ggattggggc agcttctcgg
     3121 cggatgacca gaaggcggtg atggcggcct tcacccgctt caccgtggcc aactatgcgc
     3181 acaatttcca cgattttgac gggcagagat tcgagatcga cgacaacgtg atgacgcgcg
     3241 ggccggacaa gatcgtccgc tcgcgcatcc tgcctgcgca cgacgtgccg gtgagcctgc
     3301 tgtatcggat gcacgaggtg gacggagcct ggaagatcat cgatgtg
//
LOCUS       DATDMP010000548         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2088027, whole genome shotgun sequence.
ACCESSION   DATDMP010000548 DATDMP010000000
VERSION     DATDMP010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088027"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VI195_09605"
     CDS             <1..1152
                     /locus_tag="VI195_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6824684.1"
                     /translation="IWNMMMSMSGGWFFVTVSEAISVGNTTVTLPGVGSYIALAIAQK
                     NLTAIGWAIAAMFVVILLFDQLLFRPLTASADWFRLEQETGQTPSRSWALTMMRRSAL
                     LGLVGNAFGAMLRLGMKPRAAGGALAPSEDRWSARWIDYTWYAVLLLIGVGALAKTLS
                     FVLREVRPGEIGHVVGLGFITLLRVAILIALASVIWVPIGVWVGLRPRVASIVQPIAQ
                     FLAAFPANLLFPIVVSTVVMLKLTPDIWLSPLMILGTQWYILFNVIVGASAIAPELRY
                     AGQNFGVHGWLWWKRLALPAVFPFYVTGAITASGGSWNASIVAEVANWGDQKLTAHGL
                     GAYIAEATDAGDFQRSVLGTIVLSLFVVILNRVFWRPLYARAERKFRIS"
     gene            1164..2492
                     /locus_tag="VI195_09610"
     CDS             1164..2492
                     /locus_tag="VI195_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY6824685.1"
                     /translation="MNAVAPALNLAPLLDVQGVCKSFRKPDGDQLVVLENVNLTLRPG
                     EIVGLLGRSGSGKSTLLRVIAGLDEPSSGAVSYLGQTVAGPASGIAMVFQSFALFPWL
                     TVLENVQLGLEALGVAEAEMRKRSLQAIDLIGLDGFESAYPRELSGGMRQRVGFARAL
                     VVHPNILLMDEPFSALDVLTAETLRTDFLDLWGEGQLPIKSVLLVTHNIEEAVQMCDR
                     MLIFSTHPGRVISEIMVDLPQPRHAHDPRFRALIDRVYIEMTAGPRGEARSGARAERF
                     AGVGVGTQLTHVSSNVLTGLMETVHGPPYSGRADLPAIAEEQHLEVDDLFPAAEALQM
                     LRFAEVEGGDIKLTEAGLQFARSETDERKKLFARHLVTYVPLAAHIRRVLDERASHSA
                     PKSRFIDELEDYMAEEAAEETLRTIISWGRYAELFAYDDNRQAFSLENPA"
     gene            complement(2470..2907)
                     /locus_tag="VI195_09615"
     CDS             complement(2470..2907)
                     /locus_tag="VI195_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY6824686.1"
                     /translation="MLRAPPAARLRTFFWFGLTGVTGFLVDAGVLHWLVTEWNTNLLL
                     ARGCSFTCAAATTWIINRTITFAAPRRRRRLLAAEWMAYFAASVAGGCINYLVFALAV
                     RLSPLLHEIPTMAVALGTLAGMSFNFLMYSRYVFQPVKQDSPG"
     gene            complement(2911..>3310)
                     /locus_tag="VI195_09620"
     CDS             complement(2911..>3310)
                     /locus_tag="VI195_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824687.1"
                     /translation="MRIPIAEFPTPYSERPAGSASKLRTVSDGMRIMRTIVHLLKEER
                     PLAFFGTASALLVLVALGLGIPVVVEYLHTGLVPRLPTAVLATGLMILASLSLVCGLI
                     LQTVTHGRIEMKRLNYLSIPIKPRSRANHG"
BASE COUNT          563 a         1040 c         1091 g          616 t
ORIGIN      
        1 atctggaaca tgatgatgtc catgtcaggc ggctggttct tcgtcacggt gtcggaggcc
       61 atcagcgtgg gcaacaccac cgtcacgctg cccggggtag gctcctacat cgcactcgcc
      121 attgcgcaga aaaatctcac cgccatcggc tgggcgatcg cggccatgtt cgtcgtgatc
      181 ctgttgttcg atcagttgct gttccggccg ctcactgcga gtgccgactg gtttcgcctc
      241 gaacaggaga ccgggcagac tccgagccgg tcgtgggcgc tcaccatgat gcgccgctcg
      301 gccctgctcg gtctcgtcgg caacgccttc ggcgccatgc tgcgcctggg catgaaaccg
      361 cgcgccgccg gcggcgcgct tgcgccttcc gaagaccgct ggagtgctcg ctggatcgac
      421 tacacctggt acgcggtgct gctgctcatc ggggtcggcg ctctcgccaa gaccttgagt
      481 ttcgtgctgc gcgaggtgcg ccccggcgaa atcgggcatg tcgttggcct cggcttcatc
      541 acgctcctgc gcgtagccat cctgatcgcg ctcgcgagcg tcatctgggt gccgattggc
      601 gtgtgggtgg gactgcggcc gcgcgttgcg agcatcgtgc agccgatcgc gcagttcctc
      661 gcggccttcc cggccaatct gctgtttccc atcgtggtct cgacggtcgt gatgctgaaa
      721 ctcacgccgg acatctggct gagccccctg atgattctcg gcacgcagtg gtacattctc
      781 ttcaatgtga tcgtcggcgc ttccgcgatc gcgcctgagc tgcgctacgc gggccagaac
      841 ttcggggtgc acggctggtt gtggtggaag cggctggcgc tgcccgcggt gttccccttc
      901 tatgtgaccg gcgcgatcac cgcctcgggt ggctcctgga atgcgagcat cgttgctgaa
      961 gtagcgaact ggggcgacca gaagctcacc gcgcacggcc tcggtgccta tatcgccgag
     1021 gctaccgacg ccggtgattt ccagcgtagc gtgctcggaa ctatagtgct gagcctgttc
     1081 gtggtgatcc tcaaccgggt gttctggcgg ccgctgtacg ctcgcgccga gcggaagttc
     1141 cgcatcagct gaggagagac tttgtgaacg ctgtggctcc cgcgctcaac ctcgcgccgc
     1201 tgctcgatgt gcagggcgtg tgcaaatcct tccgcaagcc cgatggcgat cagctcgtgg
     1261 tgctggagaa cgtgaacctg acgctgcgcc ctggggaaat cgtcgggctc ctcggccgct
     1321 cgggatccgg caagtcgacg ctgctgcggg tgatcgcggg tctcgatgag ccctccagcg
     1381 gggcggtgag ctatcttggg cagacggtgg ccggtcccgc ctccggtatc gccatggtgt
     1441 tccagagctt cgcgctcttc ccctggctga cggtgctgga gaacgtgcag ctcggtctcg
     1501 aggcgcttgg cgtcgccgag gcggagatgc gcaagcgctc gctgcaggcc atcgatctca
     1561 tcgggctcga tggcttcgag tctgcctatc cacgcgagct ctcgggtggc atgcgccagc
     1621 gggtcgggtt cgcgcgtgcg ctcgtggtac atccgaacat cctcctcatg gacgagccgt
     1681 tttccgcgct cgatgtcctg accgcggaga cgctgcgcac cgacttcctc gatctgtggg
     1741 gcgaagggca gctgccgatc aagtcggtgc tgctggtgac ccacaacatc gaggaggccg
     1801 tgcagatgtg cgatcgcatg ctgatcttct cgacgcatcc gggaagggtg atcagcgaga
     1861 tcatggtgga tttgccccag ccccgccacg cccatgaccc gcgcttccgc gcgctcatcg
     1921 atcgggtcta catcgagatg accgcgggac cgcgcggcga ggcacgcagc ggcgcccggg
     1981 ctgagcgctt tgccggcgtc ggtgtaggca cgcagcttac ccacgtgtcc tccaacgtgc
     2041 ttaccgggct gatggagacg gtgcatgggc ccccgtacag cggccgggcc gacctgccgg
     2101 cgattgccga ggagcagcat ctcgaggtcg atgacttgtt tccggccgcc gaggccttgc
     2161 agatgctgcg ttttgccgag gtcgagggcg gcgacattaa gctcaccgag gcgggcctgc
     2221 agtttgcgcg cagcgagacc gatgagcgca agaagctgtt cgcgcggcat ctggtcacct
     2281 acgttccgct cgccgcgcac atccgccggg tgctcgatga gcgagcgagc cacagcgctc
     2341 cgaagagccg ctttatcgat gagctcgagg actacatggc ggaagaggcg gcggaggaga
     2401 cgctgcgcac catcatcagc tggggccgct acgcggaact gttcgcctat gacgacaacc
     2461 ggcaggcgtt cagcctggag aatcctgctt gaccggctgg aagacgtacc tcgagtacat
     2521 cagaaaatta aagctcatgc cggccagcgt gccgagggcg acggccatcg ttggaatctc
     2581 gtgcagcaac ggggagaggc gcacggcgag cgcgaatacc aggtagttga tgcacccgcc
     2641 cgccacgctc gcggcgaaat atgccatcca ttcggccgcc agaagacgcc gccggcgccg
     2701 tggcgccgca aaggtgatgg tgcgattgat gatccaggta gtggcggcag cacaggtgaa
     2761 cgagcagccg cgggcgagca acagattggt attccactca gtcaccagcc aatggagcac
     2821 accggcgtcc accaggaagc cagtgacacc ggtgagccca aaccagaaaa aggttcggag
     2881 ccgagccgca ggcggtgcac gcaacacgcg tcaaccgtga ttggcacgcg aacgaggttt
     2941 gatcggtatc gagagataat tgagccgctt catctcgatg cgcccatgcg tcacggtttg
     3001 cagaatcagc ccacagacca ggctcagcga ggcgaggatc atgagcccgg tggcgagcac
     3061 agccgttggc agccgtggga ccaacccggt atgcaggtat tccaccacta ccggaatacc
     3121 aagccccaaa gccacgagca ccagtagcgc gctcgccgtg ccaaaaaatg ccaacggtcg
     3181 ttcctccttg agaaggtgca cgatcgtacg catgatgcgc atcccgtcgc ttaccgtgcg
     3241 cagcttgctg gcggagcctg cgggacgttc gctgtatggg gtgggaaact ctgcgatcgg
     3301 aatgcgcatt
//
LOCUS       DATDMP010000549         5269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_310469, whole genome shotgun sequence.
ACCESSION   DATDMP010000549 DATDMP010000000
VERSION     DATDMP010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310469"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VI195_09625"
     CDS             <1..371
                     /locus_tag="VI195_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824688.1"
                     /translation="PIKDCSKLLVYRLANYRNSRGRVIPARVIEREPSAELRAGQKDS
                     DSLPPYEVLDPILEAFIEEDLSVDEIEARGFDRATVARVLDLVKRNEYKRRQAPPGVR
                     VSRRAFGRDWRYPITSGYRR"
     gene            complement(390..1187)
                     /locus_tag="VI195_09630"
     CDS             complement(390..1187)
                     /locus_tag="VI195_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY6824689.1"
                     /translation="MLLSGPVRGALLALCVVLLALSGCRTHREDPKKITPEALYKKAH
                     KSLEAYDFNGAIKLYEQLTARFPFTDEARQARLDLIYAYYRAGEKESATDAADTFIRE
                     NPTHPRVDYAWYVKGLVDFERTPNALERLLRADLTKRPPSDARKSFAAFKTVVEQYPK
                     SEYAHDSLQRMVYLRDRLASYEVHVARYYYKRGAYVAAAQRARGAIEQYDGSPAVQEA
                     LDMLIQCYDHLQLEALATQSREVYAANYSADGRTLQADVKKSWWRFW"
     gene            1205..2221
                     /gene="rluD"
                     /locus_tag="VI195_09635"
     CDS             1205..2221
                     /gene="rluD"
                     /locus_tag="VI195_09635"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: protein motif:HMM:NF008385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEY6824690.1"
                     /translation="MASRRISARSIANPARGLTAQAPGEFRTHALSLPAEAAGLRFDQ
                     ALVRALPQYSRARLKTWIESGAVRVDGRPLRAKDRVLGGEQVRIHAHLPPQGEVQAEP
                     LPLAVVFRDRLILVIDKPAGLVVHPGAGNPRHTLQNALLALDPRLALVPRAGLIHRLD
                     KDTSGLLVIARTLEAHTVLTAALAARQVTREYLALCSGVMTGGGTIDEPIGRHRTLRT
                     RMAVRADGREAITHYRIEQRLWAHTLVRVRLETGRTHQIRVHLAHIGFPIVGDPVYGG
                     RRRVPRGCPADAAEALRAFGRQALHATRLVLRHPASGRELQFQAPLPRDMQQLIGKLA
                     AERA"
     gene            2218..3006
                     /gene="pgeF"
                     /locus_tag="VI195_09640"
     CDS             2218..3006
                     /gene="pgeF"
                     /locus_tag="VI195_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY6824691.1"
                     /translation="MNRDSPSRATAVLTPEWPAPGNVHAVFTLRGGGVSAPPFDSLNV
                     GAHVGDEVQAVEQNRRLIRAALRLPAEPVWMEQVHGVEVLDLDVEVAQGDAANAGIPS
                     ADAAITRSAGRVCVVQVADCLPVLLAARDGSAVAAAHAGWRGLAAGVLEATVRRLGVE
                     PARLIAWLGPGIGAAHFEVGEEVRSAFVAQDAAAARAFRGNPRGRWQCDLVILARARL
                     AALGVTAVFGGEWCTYADAARFFSYRRDGRCGRMAALIWREQRP"
     gene            3003..3854
                     /locus_tag="VI195_09645"
     CDS             3003..3854
                     /locus_tag="VI195_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY6824692.1"
                     /translation="MTLLLIIMLFAAVGGIASAAFALVFLWVPEERSALILPHFISFA
                     TGALLGAALLALLPEAMAAAGPAGAHGIGLALIAGLAVFFVIEKLVLWWHAHVHVHGE
                     GAAACPQHADEVHEPAGARRQQPAHMHEHARDHASGVLVLVGDSLHNALDGALIAAAF
                     LGSVQLGALTTLAVAAHEIPHRVGDFAILVQAGLSRARALLLNLATGVASVLGAVLAY
                     YGLRETQRALPYALAFAAAGFLYIAVAGLIPGLQRRADPRTSALQVLLIGAGIAVIAC
                     AERLVEQ"
     gene            3983..>5269
                     /locus_tag="VI195_09650"
     CDS             3983..>5269
                     /locus_tag="VI195_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6824693.1"
                     /translation="MLLKWLKASEATQVGTALADDVVLQTPLGSAAREQVSKPAAKEK
                     ELQRFLQKFLQRVDRDARPLQLNFFRRAKLANSFKWRLLEKGVDKEVVNELTQALVLR
                     LTASRGSAAFGDRPLAAPSARADTRNAQLLLTQGAEHLARGAHAEALECYQELVSFDP
                     RNAPARNGLGVALAQLGRYGEAEDELRRAIGVRPTFPEAHFNLGGVLQSTGRFAESEM
                     PLRRALKLKPAYLQARVSLGMSLVLLGRLQEARECYEKALSSAQRHPEALVGIGQIEA
                     LNGHFAAAEAAYRSALEVDPKLSYAWAGLVGLRKMSAADGAWLERAEEIIAGGLGPID
                     EAMLRFAMGKYCDDVGDFPRAFRNYDRANALQRMRAPPYDAAERSRFVDARVKTYTPE
                     GLRQATADASDSERPVFVVGMPRSGTSLVEQIIASHP"
BASE COUNT          820 a         1750 c         1821 g          878 t
ORIGIN      
        1 caccgatcaa ggactgcagc aagttgctgg tatatcgcct ggcgaactac cgcaactcac
       61 gcggccgcgt cataccggcg cgcgtgatcg agcgggagcc atccgccgag ctgcgcgccg
      121 ggcagaaaga cagcgattcg ctgcccccct acgaagtgct cgacccgatc ctcgaagcct
      181 tcatcgaaga ggacctgtcg gtggatgaga tcgaagcgcg cggcttcgac cgcgccaccg
      241 tggctcgagt gctcgacctg gtgaagcgca acgagtacaa gcgccggcag gcgccgcctg
      301 gcgtgcgcgt gagccgccgt gccttcggac gcgattggcg ttacccgatc acgagcggct
      361 accgccgctg aggggcggca gcgggtgcgc taccagaacc tccaccagga cttcttgacg
      421 tctgcctgga gcgtacggcc gtcagcggag tagttggcgg catacacttc gcggctctgc
      481 gtagcgagcg cctcgagctg caggtggtcg tagcactgga tcagcatgtc gagcgcttcc
      541 tgcacggcgg gcgagccgtc gtactgctcg atggcgcccc gggcacgctg ggccgccgca
      601 acgtaggcgc cgcgcttgta gtagtagcgc gcgacgtgca cctcgtagct cgccaggcga
      661 tcgcgcaggt aaaccatgcg ctgcagcgaa tcgtgggcgt actcgctctt cgggtactgc
      721 tcgaccacgg tcttgaatgc ggcaaatgac ttgcgcgcgt cgctcggcgg ccgcttggtc
      781 aggtccgcgc gcagcaaacg ctcgagtgca tttggcgtac gctcgaagtc gacgagcccc
      841 ttgacgtacc acgcgtagtc cacgcgtgga tgtgtcgggt tctcgcggat gaaggtgtcg
      901 gcggcatcgg tggcggactc cttctcaccg gcgcggtagt atgcatagat caggtcgagt
      961 cgcgcctggc gggcctcgtc ggtgaacggg aagcgcgccg tcagctgctc gtacaatttg
     1021 atggcaccgt tgaagtcgta cgcctcgagg ctcttgtgcg ccttcttgta gagcgcctcc
     1081 ggggtaatct tcttcgggtc ctcgcggtgc gtgcggcagc cggataatgc aagcagcacg
     1141 acacacaacg cgagcagggc gccgcgtacc ggaccagaga gcaacatcgg gaaagggcct
     1201 ttctgtggct tcacgccgta tcagtgcgcg gagtatagcg aatcccgccc gtggactgac
     1261 tgcgcaagcc ccgggggagt tccgtacgca cgcgctcagc ctgcccgccg aggccgcggg
     1321 tctgcggttc gaccaggctc tggtgcgcgc gttgccgcaa tactcgcgtg cccggctcaa
     1381 gacctggatc gaatcgggtg cggtgcgggt cgatggccgg ccgctgcgcg ccaaggatcg
     1441 cgtgctggga ggcgaacagg tgcgcatcca cgcccacttg cccccgcagg gcgaggtgca
     1501 ggccgagccc ctgccgctcg ccgtcgtatt cagggaccgc ttgatcctgg tgatcgataa
     1561 gccggcggga ctcgtggtcc acccgggagc cggcaaccca cgccacacct tgcagaacgc
     1621 gttgctcgcc ctcgatccac ggcttgccct ggtaccgcgc gccggcctga tccatcgact
     1681 cgataaggac acgagcgggc tgctcgtcat cgcgcgcacc ctcgaagcgc acaccgtgct
     1741 gacagcggca ctcgcggcac ggcaggtcac gcgcgagtac ctcgcgctgt gcagcggagt
     1801 catgaccggc ggaggcacca tcgatgagcc catcggtcgt caccgcacgc tgcgcacgcg
     1861 catggcggtg cgagcggacg gacgcgaggc catcacgcac taccggatcg agcagcgtct
     1921 gtgggcccac actctggtgc gcgtcaggct cgagaccgga cgcacgcatc agatccgcgt
     1981 ccacctggcc cacatcggtt tcccgatcgt cggcgatccg gtctacggcg gacgccgccg
     2041 tgtcccgcgc ggctgcccag cggatgcggc cgaggcgttg cgtgccttcg ggcgccaggc
     2101 gctgcacgcc acacggctcg tgctgcggca tccggcgagc ggtcgcgagc tgcagttcca
     2161 ggcgccactg ccgcgggaca tgcagcagct catcggcaag ctcgcagcgg aacgcgcgtg
     2221 aaccgggact cgccatcgcg agcgaccgca gtcctcaccc cggaatggcc ggccccgggg
     2281 aatgttcacg ccgtattcac gctgcgcggc ggcggcgtga gcgcgccgcc tttcgacagc
     2341 ctcaatgtag gagcgcacgt cggcgatgag gtccaggcag tggagcagaa ccgccggctc
     2401 attcgggcgg cactgcggct gccagccgaa cccgtctgga tggaacaggt gcacggcgtc
     2461 gaggtactcg acctggatgt ggaggtagcg caaggcgacg ctgccaacgc cggcatcccc
     2521 agcgcggacg ccgccatcac ccgcagcgcg ggacgcgtgt gcgtcgtgca ggttgcggac
     2581 tgcctgccgg tgctgctggc tgctcgcgac ggctcggcgg ttgccgcggc ccacgccggc
     2641 tggcgcggtc tcgcggcggg tgtgctggag gccaccgtgc gccgactggg ggtcgagccc
     2701 gcacggctca tcgcctggct cggtccggga atcggcgcgg cgcacttcga ggtcggtgaa
     2761 gaggtgcgca gcgcttttgt ggcgcaggat gccgccgccg cacgcgcgtt ccgcggcaac
     2821 ccgcgcgggc gctggcaatg tgacctggtg atcctcgccc gggcgcgact cgctgcgctc
     2881 ggtgtcactg cggtattcgg cggcgagtgg tgtacctacg ccgatgcggc acgcttcttc
     2941 tcttaccgcc gggatggccg ttgcggacgc atggcggcac tcatctggcg tgaacagcgg
     3001 ccatgactct gctgctgatc atcatgctgt tcgccgccgt tggcggcatc gcgagcgcgg
     3061 cgttcgcgct cgtgttcctg tgggtacccg aggagcgcag cgcgctgatc ctgccgcact
     3121 tcatcagttt tgcgaccgga gcgctgctcg gagcagcgct gctcgcactc cttcccgagg
     3181 cgatggccgc ggcaggcccc gcaggagccc atggcatcgg gctcgcgctc atcgccggcc
     3241 tcgcggtgtt tttcgtgatc gagaaactgg tgctgtggtg gcacgcgcac gtgcacgtgc
     3301 acggcgaggg tgccgccgcc tgtccgcagc acgctgacga agtgcacgag cctgcgggcg
     3361 cgcggcggca gcaacccgct cacatgcacg agcatgcccg tgatcacgcc tcgggtgtgc
     3421 tggtgctcgt cggcgatagc ctgcacaacg cgctcgatgg cgcgctcatc gcggccgcgt
     3481 tcctcggcag cgtgcagctg ggagccctca cgacgctcgc ggtcgcagcc catgaaattc
     3541 cgcatcgggt gggggatttc gccattctgg tgcaggcggg gctgtcacgc gcgcgtgcgc
     3601 tgctgctcaa tctcgctacc ggggtcgcct cggtgctcgg cgcagtgctg gcgtactacg
     3661 gcctgcgcga gacgcagcgt gcgctaccct acgcactggc cttcgccgcc gcgggctttc
     3721 tctacatcgc ggttgccggt ctcatcccgg gactgcagcg tcgcgccgac ccgcgcacca
     3781 gtgccttgca ggtgctgctc atcggggccg gcatcgccgt catcgcctgc gccgagcgac
     3841 tcgtcgagca atgacggtcg acggcctcta tccgcgtttc gattgtgatg acgctcacta
     3901 atgagcggct tctcacaaag catcgagcgg aacttggtac catttaacgt aagcacggga
     3961 tgctgagggc gggttaccac gcatgctgct gaaatggttg aaggcgagtg aggccaccca
     4021 ggtcggcacc gcgttggccg acgatgtcgt gctgcagacc ccgctaggtt cggcggcgcg
     4081 ggagcaagtc agcaaacccg cggccaagga aaaggagttg cagcggttcc tgcagaagtt
     4141 tctgcagcgc gtcgaccgcg acgcgcgccc gctgcaattg aacttcttca ggagagccaa
     4201 gctcgccaac tccttcaagt ggcgcctgct cgagaagggc gtggacaagg aggtcgtcaa
     4261 cgagctgacg caggcgctgg tgctgcgtct caccgcgagc cgtggcagtg cggccttcgg
     4321 cgacagacca ctcgcggccc cgagcgcacg cgcggacacg cgcaacgccc agctgctgct
     4381 gactcagggc gccgagcatc tggcgcgggg ggctcatgcc gaggcgctgg agtgttacca
     4441 ggaactggtg agcttcgatc cacgtaatgc tccagcgcgc aacggtctcg gggtcgccct
     4501 ggctcagctc ggacgctatg gcgaggccga ggatgaactg cgtcgcgcga tcggagtcag
     4561 gcccactttc ccggaggcac atttcaatct cggcggggtc ctgcaatcga cgggccgctt
     4621 cgccgaatcc gagatgcccc tgcggcgcgc actgaagttg aagccggcgt acctgcaggc
     4681 acgggtgagt ctcggcatga gtctcgtgct gctgggccgt ctccaggagg cccgcgagtg
     4741 ttacgagaaa gccctcagtt cggcgcagcg ccatccggag gcactggttg gaatcgggca
     4801 gatcgaggcg ttgaacggac attttgccgc cgcggaggcc gcgtaccgca gcgccctcga
     4861 ggtcgatccg aaattgtcgt acgcctgggc agggctcgtc ggcctgcgca agatgagcgc
     4921 cgcggacggc gcctggctcg agcgtgcgga ggagatcatc gcgggggggc tcggacccat
     4981 cgacgaagcc atgctgcgct tcgcaatggg caagtattgc gatgatgtcg gagattttcc
     5041 gcgggcgttc cgcaactacg accgcgccaa cgcgctgcaa agaatgcgcg cgccgcccta
     5101 cgatgcagcg gagcgctcgc gcttcgtcga tgctcgggtc aagacctata cccctgaagg
     5161 gctgaggcaa gcgacggccg atgcctcgga ctccgagcgg ccggtcttcg tggtcggcat
     5221 gcctcgctcg gggacctcgc tcgtcgagca gatcatcgcc tcgcacccc
//
LOCUS       DATDMP010000550         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_421622, whole genome shotgun sequence.
ACCESSION   DATDMP010000550 DATDMP010000000
VERSION     DATDMP010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421622"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1090
                     /locus_tag="VI195_09655"
     CDS             80..1090
                     /locus_tag="VI195_09655"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEY6824694.1"
                     /translation="MKILLTGAAGFIGAQTAHCLLTAGHEVIGLDNLNDYYDVGLKQA
                     RLARLTAPGFRFVKLDLADDDAIAQLFARERFARVVHLAAQAGVRHSLKEPHAYIRSN
                     ITGTLNVLEGARHHGVEHLVYASTSSVYGANTDMPFSVHRIADHPLSLYGATKRANEL
                     MAHSYSALFALPTTGLRFFTVYGPWGRPDMALFTFTRNILDGRPIDVYNHGHHARDFT
                     YVDDIAEGVVRACERIAQPDPRWNSRAPDPATSAAPFRLYNIGNHQPVQLMRYIEVIE
                     ECLGRKAQLNLLPLQPGDVPETFADIDDLVHDVGYRPATPIEVGVGRFVDWFCEYYGY
                     RR"
     gene            1161..>2043
                     /locus_tag="VI195_09660"
     CDS             1161..>2043
                     /locus_tag="VI195_09660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY6824695.1"
                     /translation="MAVSTSRSRPAMQVPLLDLKPQYQRLAADIQAAIGRVCASQHFI
                     LGPAVRELETSVAGYCQCRHGIGVSSGTDALLLALMALGIGAGDAVITSPFTFFATAG
                     TIARTGARPLFCDIDPLTFNISAGAVAQLLEGECERREGELIHRASGTRVRALMPVHL
                     YGQLADMSPLVDLARRYQLKIIEDAAQAIGAADEQQRRAGSFGDVGCLSFFPTKNLGA
                     FGDAGMCVTNDAALAERMEILRVHGGKPKYYHALIGGNFRIDELQAAVLNVKLPHLDD
                     WTAARQRNAAFYQRALAR"
BASE COUNT          336 a          724 c          644 g          339 t
ORIGIN      
        1 tacgccgcct gtgccgccgc aaacacgtgc tctacgacat caagcatgtg tttccggccg
       61 cagcggtcga tggacggctg tgaagatact gctcaccggc gcggccggct tcatcggcgc
      121 gcagaccgcg cactgcctgc tcacggccgg gcacgaagtc atcgggctcg acaatctcaa
      181 cgactactac gacgtaggtc tcaagcaggc gcggctcgca cgcctcaccg cgccgggctt
      241 ccggttcgtc aagctcgacc tggccgacga tgatgcgatc gcacagctgt tcgcgcgcga
      301 gcgcttcgcg cgcgtcgtgc accttgccgc ccaggcgggg gtgcgccact cgctcaagga
      361 accgcacgcc tacattcgca gcaacatcac cggcacgctg aacgtgctcg aaggcgcgcg
      421 ccaccacggc gtcgagcacc tggtgtatgc ctcgacgagc tcggtgtacg gcgccaacac
      481 cgacatgcct ttctcggtac accgcatcgc cgaccatccg ttatcgctct acggcgccac
      541 caagcgcgcg aatgagctca tggcgcacag ctactcggcg ctgttcgcgc tgccgaccac
      601 ggggctgcgg ttcttcacgg tgtacggtcc ctggggccgt ccggacatgg cattgttcac
      661 cttcacccgc aacattctcg atggccgccc catcgatgtg tacaaccacg gccatcatgc
      721 gcgcgatttc acctacgtag acgacatcgc cgagggagtg gtacgcgcct gcgagcgcat
      781 cgcgcaaccc gatccgcgct ggaacagtcg tgcgcccgac ccggcgacca gcgcggcgcc
      841 gttccgcctg tacaacatcg gcaaccacca gccggtgcag ctcatgcgct acatcgaagt
      901 catcgaggaa tgcctcgggc gcaaggcgca gctcaacctg ctgccgctgc agccgggtga
      961 tgtgccggag accttcgccg acatcgacga cctggtgcac gatgtcggct accgtcccgc
     1021 gaccccgatc gaggtggggg tgggccgctt cgtcgactgg ttctgcgagt actacggcta
     1081 ccggcgttga ccagccgcgc ggttgcgcga ccccggggta gaatcgccgc tcccgccgca
     1141 agcgcacccg gaggcgcctc atggccgtca gcacctcgcg atcccgacct gcgatgcagg
     1201 tgccgctgct cgatctgaag ccgcaatacc agcggctcgc tgccgacata caggcggcga
     1261 tcggccgcgt gtgcgcgagt cagcatttca tcctggggcc ggcggtgcgg gagctcgaga
     1321 cgagcgtcgc cgggtactgc cagtgtcgcc acggcatcgg tgtctcttcg ggcaccgacg
     1381 cactgctgct cgccctcatg gcgctgggta tcggcgcggg ggatgcggtg attaccagcc
     1441 cgttcacttt cttcgccacc gccggcacca tcgcccgcac cggcgcgcgg ccgctgttct
     1501 gcgacatcga tccgctcacc ttcaacatca gcgccggcgc ggtcgcgcag ctgctcgagg
     1561 gtgaatgtga gcggcgggaa ggcgagctca tccatcgcgc cagcggtaca cgggtgcgcg
     1621 ccctcatgcc ggtgcatctg tacgggcagc tcgctgacat gtcaccgctc gtggatctgg
     1681 cgcgccgcta ccaactcaag atcatcgagg atgctgcgca ggcgatcggc gccgccgatg
     1741 agcagcagcg gcgcgccggc agcttcggcg atgtcggctg cctgtcgttt ttccccacca
     1801 agaatctcgg tgccttcggc gatgccggga tgtgcgtgac caacgacgcg gcactcgccg
     1861 agcgcatgga gatcctgcgc gtgcacggcg ggaaacccaa gtactaccat gcgctcatcg
     1921 gcggcaattt ccgcatcgat gagctgcagg cagcggtgct caacgtcaag ctgccgcatc
     1981 tggatgactg gaccgcggca cggcaacgca acgccgcctt ctaccagcgc gctctggcgc
     2041 gcg
//
LOCUS       DATDMP010000551         3033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2088582, whole genome shotgun sequence.
ACCESSION   DATDMP010000551 DATDMP010000000
VERSION     DATDMP010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088582"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VI195_09665"
     CDS             complement(<1..224)
                     /locus_tag="VI195_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824696.1"
                     /translation="MIMHRADQTLAIRALKSAAPYIRMYKGKTFVVKVGGGVFADSAA
                     TRMLVEQIGILHYFGVRVVLVHGGGPQLTEV"
     gene            complement(250..1245)
                     /locus_tag="VI195_09670"
     CDS             complement(250..1245)
                     /locus_tag="VI195_09670"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HEY6824697.1"
                     /translation="MKRFLDLADFARDEVLALLELARRLERQPEPRALAGKLLALVFF
                     NPSLRTLASFQAAMARLGGQSLVVNPGQGSWQMETRGGVVMDGAAAEHVREGIPVLAS
                     YCDALGIRLFAEGRDLASDLAESSFSSIASLIDKPLVNLESAVNHPCQALADWKTLDD
                     LAVPRGGRFVLSWAYHPRALPLAVPAAALHMAALRGMEVTVLRPEGFALPNEIMERAR
                     RAAAGSGGSVHETSERAQALRGAHVLYVKEWGAPGCYGDAAADAQLRSTLRSWCVRND
                     WLTASPDCRVMHCLPVRRNSAIADEVLDSPRSVVQREAGNRLPVQMAVLHRLLRP"
     gene            complement(1242..1406)
                     /locus_tag="VI195_09675"
     CDS             complement(1242..1406)
                     /locus_tag="VI195_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824698.1"
                     /translation="MHTTAATTAPCPAAECGILAGTSNDSHVLRLLPAFILEDEHVEL
                     LRDALRELPA"
     gene            complement(1417..2478)
                     /gene="argC"
                     /locus_tag="VI195_09680"
     CDS             complement(1417..2478)
                     /gene="argC"
                     /locus_tag="VI195_09680"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY6824699.1"
                     /translation="MSIPIPAVVLGGTGYVAGELLRLLAVHPRLRLAAVMSESAAGEP
                     VAAAFPHLRSSYPQERFRSEAEVRKVLCDAAGAALFSAAPHGVSASLIERLLAAAERA
                     GTEPRVVDTSADFRYATAEAYSSVYGAAHAAPGRLAQFSCALPEHLPRPRTRHIGHPG
                     CFATAILLASVPLLAAGYVSPTLFVSGVTGSTGSGRKPVDGTHHPLRHADLYSYGALA
                     HRHVPEITACARAATGIEAEFAFVPHSGPFARGIHVTVQAALRRPIDRAALLGTLREF
                     YSGAPFVQVSEQAPRVKDVAASNYAHLSAAARGNTVAVLCAIDNLVKGAAGGAIQWMT
                     RLLELPETAGLTSPAPGWS"
     gene            complement(2475..>3033)
                     /locus_tag="VI195_09685"
     CDS             complement(2475..>3033)
                     /locus_tag="VI195_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824700.1"
                     /translation="KTAAACAVIARMRHRGLTVDAFKATGVSLRRDILAMEDAGARHC
                     AIFTDLGVVTTTRVNGPALTRTMLSELAAGKPDALLFELGDGILGTYGVDAILECPDI
                     RSALSAVVLSANDPVAAWGGVKLLRERFAIEPCVITGPATDNQVGIEIIAAQMGVPAV
                     NALSSGAALGDRVIEALGLADTALP"
BASE COUNT          478 a         1038 c         1092 g          425 t
ORIGIN      
        1 acctcggtga gctgcgggcc gccgccgtgc acgagcacca cccgcacccc gaagtaatgc
       61 aggatgccga tctgctccac cagcatgcgc gtggcggcgc tgtcggcgaa cacgccgccg
      121 ccgactttca ccacgaaggt cttgcccttg tacatgcgga tgtagggcgc ggcgctcttc
      181 agtgcgcgga tggcgagggt ctggtcggct ctgtgcatga tcatgggggc gtccgggccg
      241 tgggaggtgt caggggcgca gcaggcggtg cagcaccgcc atctggacgg gcagccggtt
      301 gccggcttcg cgctgcacca cgctgcgcgg gctgtcgagt acttcatccg cgatggcgga
      361 attgcgccgc acgggcaggc agtgcatcac ccggcaatcg ggcgacgccg tgagccagtc
      421 gttgcgcacg caccagctgc gcagcgtgct gcgcagctgc gcatccgccg ccgcatcgcc
      481 atagcagccc ggcgcacccc actcctttac atagagcaca tgagcgccgc gcagcgcctg
      541 cgctcgctca ctcgtctcgt gcaccgaccc gccggagccc gcggcggcac gccgtgcgcg
      601 ctccatgatc tcgttcggca gtgcgaagcc ctccgggcgc aacaccgtca cttccatgcc
      661 gcgcagcgcc gccatgtgca gcgcagccgc cggcacggcg agcggcaggg cacgcgggtg
      721 atacgcccac gagagcacga agcgcccgcc acgcggtacc gccaggtcat ccagggtctt
      781 ccagtcggcg agggcctggc acggatggtt gaccgccgac tcgaggttca ccaacggctt
      841 gtcgatgaga ctcgcgatgc tggaaaagct gctctcggcg agatcgctgg cgagatcgcg
      901 cccttcggcg aacaagcgga tgccgagcgc gtcgcagtag gaggcgagca ccgggatgcc
      961 ctcgcggaca tgttccgcgg ccgcaccatc catgaccacc ccgccgcggg tctccatctg
     1021 ccagcttccc tggccgggat tgacgaccag ggactgaccg ccgaggcgcg ccatcgcggc
     1081 ctggaaggat gcgagcgtgc gcagcgaggg attgaagaac acgagcgcga gcagcttgcc
     1141 tgcgagtgcg cgtggttcgg gttgccgctc gaggcgccgc gcgagttcca gcagtgccaa
     1201 cacctcatcg cgcgcgaaat cggcgaggtc gaggaaacgc ttcatgcggg cagctcgcgc
     1261 agcgcatcgc gcaacaactc gacgtgctcg tcctcgagaa tgaacgcggg cagcaggcgc
     1321 agtacgtgac tgtcattgga ggtgccggcg aggatgccgc actcggctgc agggcagggc
     1381 gccgtggttg cagcggtggt gtgcatgcgc ttcctgtcag ctccagcccg gggcggggct
     1441 ggtgaggcct gcggtctccg gcagttccag taggcgggtc atccactgga tggcgccgcc
     1501 cgctgcgccc ttgacgaggt tgtcgatggc gcacagcacc gcgacggtgt tgccgcgcgc
     1561 ggcagcggag aggtgcgcgt aattgctcgc ggcgacatcc ttcacgcgcg gcgcctgctc
     1621 gctcacctgc acgaatggcg cgccggagta gaactcgcgc agcgtgccca gcagggccgc
     1681 gcggtcgatc ggccggcgca gtgccgcctg taccgtcacg tgaatgccgc gcgcgaaggg
     1741 ccccgaatgt ggcacgaagg cgaactccgc ctcgatgccg gtcgccgcac gcgcacaggc
     1801 ggtgatctcg ggtacatggc gatgggcaag tgcgccgtag ctgtagagat cggcatgccg
     1861 cagcggatgg tgcgtgccat cgaccggttt tctgcccgag ccggtactgc cggtgacgcc
     1921 gctcacgaac agcgtcgggc tgacgtaccc ggcagccagc agcggcacgc tcgcgagcag
     1981 gatggcagtg gcgaagcagc ccggatgccc gatgtgtcgg gtgcgcggcc gcggcagatg
     2041 ctcgggcagc gcgcagctga actgggcgag gcggccgggc gctgcgtgcg ccgcgccgta
     2101 cacggatgag taagcctcgg cagtcgcata acggaaatcg gcggaggtgt cgaccacacg
     2161 tggctcggtg ccggcgcgct ccgcggccgc gagcaagcgc tcgatgaggc tggccgacac
     2221 cccgtgcggc gccgccgaga acagcgccgc gcccgcagcg tcgcacagca ccttgcgcac
     2281 ctccgcctcg gagcggaagc gctcctgcgg gtagctcgag cgcaggtgcg gaaacgccgc
     2341 cgccaccggc tcgcccgcgg cgctctcgga catgaccgcg gccagtctca gccgcggatg
     2401 cacggcgagc agacgcaaca gctccccggc aacgtagccg gtgccaccga gcaccacggc
     2461 ggggatgggt atgctcacgg cagcgcagtg tcggcgaggc cgagggcctc gatgacccgg
     2521 tcgccgagcg cggccccgct gctgagggcg ttgactgccg gcacccccat ctgcgcggcg
     2581 atgatctcga tgccgacctg gttatcggtg gcggggccgg tgatcacgca cggctcgatg
     2641 gcgaagcgct cgcgcagcag cttcacgccg ccccaggccg cgaccggatc gttcgccgac
     2701 agcaccaccg cagagagcgc gctgcggatg tccgggcact cgagaatggc gtcgactccg
     2761 taggtgccga ggatgccatc gccgagctcg aagagcagcg catcgggctt gcccgcggcg
     2821 agctcgctca gcatggtgcg ggtgagcgcc gggccgttga cgcgtgtggt cgtgaccact
     2881 ccgaggtcgg tgaaaatcgc acagtgccgc gcaccggcgt cctccatcgc gaggatgtca
     2941 cgccgcaacg agacgccggt cgccttgaag gcatcgaccg tgagtccgcg atggcgcatg
     3001 cgcgcgatga cggcacaggc ggcggcggtc ttg
//
LOCUS       DATDMP010000552         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1310712, whole genome shotgun sequence.
ACCESSION   DATDMP010000552 DATDMP010000000
VERSION     DATDMP010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310712"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VI195_09690"
     CDS             complement(<1..573)
                     /locus_tag="VI195_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6824701.1"
                     /translation="MTLFSAPADPWSHRTRIVLAEKGISIDIVSVEAGRFPEDLLDLN
                     PYHSVPTLVDRDLVLYDSRVIIEYLDERFPHPPLMPVDPVTRAQFRLALYRIERDWYS
                     LARQIDSEPDKKQTVKLKKILRDTVLQSTDLFKVKPYFLSDEFSLVDATIAPILWRLP
                     YYEIDLSPQAQPIERYAHLVFARPAFREALS"
     gene            complement(651..1514)
                     /locus_tag="VI195_09695"
     CDS             complement(651..1514)
                     /locus_tag="VI195_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY6824702.1"
                     /translation="MRTDAQCARLQARATARGRIAAGIGPALFAAALLLALGCGAAQA
                     ADEPAAGSEGAKFGADWESWRPNTDVTDMPSVQRGARNFVSYCLGCHSVKYERWSRLA
                     DDLSIPPDLLQKRLLPPGDKSTDYILTTMPSTDAETWFGKTPPDLTLMARARGRSYLY
                     QFLKTFYVDPSRQTGANNLRLPTTAMPDILSELEGLKRAVYRDVAGKGEGGQATHEQV
                     FDHFETVAPGRLGETEYDQFVRDTVNFLDYVSEPTQAARRALGVWVVLFLLVFTWLAW
                     LVKREYWRDVH"
     gene            complement(1504..>2087)
                     /locus_tag="VI195_09700"
     CDS             complement(1504..>2087)
                     /locus_tag="VI195_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY6824703.1"
                     /translation="NPDGIEIKDKVGPDGKPLDGIPFHPYYTVKDLFGVGVFAVLFAI
                     VVFFVPTLGGLFLESPNFEPANPLSTPEHIAPAWYFTPYYAILRAVPNQRLGALLLLL
                     AVMAFLFLPWLDRSRVKSMRYKGWMSRSALALFTVSFLALGYLGLQPAVGVYVVLARI
                     FAAIYFAFFLLMPFYSRLDPVKPVPERVTYHAH"
BASE COUNT          399 a          647 c          698 g          343 t
ORIGIN      
        1 ggacagagct tcgcgaaacg caggacgggc gaataccagg tgcgcgtatc tctcgatcgg
       61 ttgcgcctgc ggtgacaggt cgatctcata atacggcagc cgccacagaa taggcgctat
      121 cgtggcatcg accagcgaga attcgtcgga gaggaagtac ggcttcacct tgaacaggtc
      181 cgtgctctgc agaacggtgt cacgcaggat cttcttcaat ttcacggtct gcttcttgtc
      241 gggctcggaa tcgatctggc gcgcgaggct gtaccagtcg cgctcgatgc gatagagcgc
      301 gaggcggaac tgcgcgcgcg tcaccgggtc gaccggcatc agcggcgggt gcgggaagcg
      361 ctcatcgaga tactcgatga tgacgcgcga gtcatacagc accaggtcac ggtcgaccag
      421 cgtcggtacg ctgtgatagg gattgaggtc gagcagatcc tcgggaaagc gtccggcctc
      481 gacactcacg atgtcgatgc tgatgccttt ctccgccagc acgatgcgcg tgcgatgact
      541 ccacgggtcg gcgggcgcgg agaacagggt catgatcgat cgtctgctcg acaagtttgc
      601 gctcctgcgg accctgtatc agcgcacgcg ctgcgcgccc gctgcgccga tcagtgaacg
      661 tctctccagt attcgcgctt cacgagccat gcgagccagg tgaacaccag cagaaacagc
      721 accacccaca ctccgagtgc gcggcgcgcg gcctgcgtcg gctcgctgac gtagtcgaga
      781 aagttgaccg tatcgcgcac gaactgatcg tactcggtct cgcccaggcg gccgggggcg
      841 accgtctcaa aatggtcgaa tacctgctca tgggtcgcct gtccgccttc gcccttgccc
      901 gcgacgtcac ggtacaccgc ccgcttgagt ccctcgagct ccgagagaat gtcgggcatc
      961 gcggtggtcg gcagccgcag attgttggcg ccggtctggc ggctcggatc cacgtagaag
     1021 gtcttgagga actgatacag gtagctgcgg ccgcgcgcac gcgccatgag cgtcaggtcg
     1081 ggtggagtct tgccgaacca ggtctcggca tcggtcgacg gcatggtggt gaggatgtag
     1141 tcggtgctct tatcgccggg cggcaacagc ctcttctgca gcaggtccgg aggtatcgag
     1201 agatcgtcgg cgaggcgcga ccagcgctcg tatttcaccg agtgacagcc gaggcagtag
     1261 ctgacgaagt tacgcgcacc gcgttgcacc gaaggcatgt cggtgacgtc ggtgttggga
     1321 cgccagctct cccagtcggc gccgaacttg gcaccctcgc tccccgcggc gggctcgtcg
     1381 gccgcctggg ccgccccgca accgagcgcc agcagcagcg cggcggcaaa cagggccggc
     1441 ccgatgccgg ctgcgatccg gccgcgcgcc gtagcacgcg cttgcaggcg cgcgcactgc
     1501 gcgtcagtgc gcatggtagg tcacccgttc gggtaccggc ttcaccggat cgagcctgct
     1561 gtagaaaggc atgagcagga aaaacgcgaa atagatcgct gcgaagatgc gcgccagcac
     1621 cacgtacacg ccgaccgccg gctgcagacc gaggtaaccg agcgccagga aggacaccgt
     1681 gaacagcgcg agcgcgctgc gcgacatcca acccttgtag cgcatggact tcacgcgtga
     1741 ccggtcaagc cacggcagga acaggaaggc catgaccgcg agcagcaaca gcagcgcccc
     1801 cagcctctga ttgggtaccg cgcgcaggat tgcgtagtag ggagtgaagt accacgccgg
     1861 cgcgatgtgc tcgggcgtcg acagcgggtt ggcgggctcg aagttcggcg actcgaggaa
     1921 cagtccgccg agggtcggca cgaagaacac gacgatcgcg aacagcacgg cgaacacacc
     1981 gacgccgaag agatccttca cggtgtagta gggatggaac gggatgccat cgagcggctt
     2041 gccgtcgggg ccgaccttgt ccttgatctc gataccgtcc ggattgt
//
LOCUS       DATDMP010000553         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1089112, whole genome shotgun sequence.
ACCESSION   DATDMP010000553 DATDMP010000000
VERSION     DATDMP010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089112"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VI195_09705"
     CDS             complement(<1..192)
                     /locus_tag="VI195_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824704.1"
                     /translation="MRLLVYNIRYATGTGPAFHLPIPGAGYLRSNRKVLTGITEFLRA
                     EQPDIVGLIEVDTGSIRTGM"
     gene            378..830
                     /locus_tag="VI195_09710"
     CDS             378..830
                     /locus_tag="VI195_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824705.1"
                     /translation="MSQANPLRLFVTHAWETSDDYLRVFEYLESARNFFYRNYSTPEQ
                     RPSGDKEALREDLRRQIAPVEAVIALSSMFEPQRELLTFELLYAQANHKAVVLMKPFG
                     ARKEVPKEILDLASEVVDWDERALVDAIRRQARHEETTRWDTIEFKLD"
     gene            complement(817..1284)
                     /locus_tag="VI195_09715"
     CDS             complement(817..1284)
                     /locus_tag="VI195_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824706.1"
                     /translation="MSTAWQQLEAAALTLVRSGPIKDRLADAYRKHLSLVNVEELPEG
                     LRAEFRACHDALTREAPLRGEDAVRATVRKLSSHEADELACTVVRLFAAMVREGARAE
                     VTENSELSVSGSTAASTRAKMLKSVPQVISLRAAEAESGRATRPGSGRPVSRA"
     gene            complement(1726..2220)
                     /locus_tag="VI195_09720"
     CDS             complement(1726..2220)
                     /locus_tag="VI195_09720"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY6824707.1"
                     /translation="MQQHAVTHGSRGFTLMELMIVVVIATILVSIAVPSYLSQVRQSR
                     RTQAKTAVLDLAGREERYFSTNGALYSTTPADLGYAGAFPALVAPDNYYRITVCTAAA
                     AAATPACDPNPNAPPGPSYYITATPVGGSSQAADTQCQSFSVDSTGQQFSTGTLTSQE
                     CWAQ"
     gene            complement(2248..>4113)
                     /locus_tag="VI195_09725"
     CDS             complement(2248..>4113)
                     /locus_tag="VI195_09725"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY6824708.1"
                     /translation="PSYTVFARERYLRGSRSDEINSSGVGLFRARTDVLADIVDSSPT
                     WVGPPTAPYTAAWNDRLYPGANNPESANGAQTYAAYVTAEQTRLNVVYVGANDGLVHG
                     FRSGSYDANGNFVNNALTPNDGAEVLAYMPGAVLQTIHSMTNSVDYANAQYAHNFFVD
                     ATPTSGDLFYGGQWHTWLVGGLAAGGAAIYALDVTNPSAVNFTEGNAASLVVGEWTSS
                     TIACTNVTNCGNNLGNTYGTPQIRRLHNGNWAVIFGNGYGSVSGDAGIYIMTLDPNTA
                     ARTVYYLSTATGSAANPNGIAYASAADLDGDHITDYVYAGDLQGHVWRFDLTSNSPAN
                     WAVTPGPLFTTPAGQPITTQVAIAGGSLTPGAAPLVMVLFGTGQKTPLTTSSASSYAS
                     GTQALYGVWDWNMSAWDAMSSAHYASLAPAATVNLASPNYTIAQSNLQAQLVTVNAAT
                     QDREIANNATICWAGGSACASNNNKYGWYLTLPGAQEQIIFNPELVAQALTVNSIVPA
                     PNNPVSCTQTSDTGFTYVVSAMTGGAFQQVFLPPSEAANPTVNNNPAYTDSIAVGMLT
                     NATGSSFVTGNSAGTAFLVFETNSTNGGSTIGNGTLGLNLPPNTTGRRLSWIERR"
BASE COUNT          689 a         1254 c         1415 g          755 t
ORIGIN      
        1 catgccggtg cggatcgagc cggtgtccac ctcgatcagg ccgacgatgt ccggctgctc
       61 cgcacgcagg aactcggtga taccggtgag caccttgcgg ttactgcgca aatacccggc
      121 acccggaatg ggcaagtgaa aagccgggcc cgtaccggtg gcgtagcgaa tgttgtagac
      181 gaggagtcgc accttgcagg ggaggtcagc ggtgaggatt taagggccgc aaggagcgga
      241 ttctatcgcg cggcggcgcc gcgtgcggtg ccagggctat cgccctgcat gaagcgagcc
      301 gcgacgcccg cgcccacgcg cagaccggca ggggcggaag tttcgctgcg catggccttg
      361 tgccagactt ggccgtaatg tcgcaggcca atccgcttcg gctcttcgtc acgcacgcct
      421 gggagacgag cgacgattac ctgcgtgttt tcgagtacct ggaaagcgcg cgcaacttct
      481 tctaccgcaa ctacagcacc ccggagcagc gcccgagcgg ggacaaggag gcgctgcgcg
      541 aggatctgcg ccggcagatc gcgccggtcg aggcggtgat tgcactctcg agcatgttcg
      601 agccgcagcg ggagctgctc accttcgagc tgctgtatgc gcaggcgaac cacaaggcgg
      661 tggtgctcat gaaacccttc ggcgctcgca aggaagtgcc aaaggagatc ctcgacctcg
      721 ccagcgaggt ggtcgactgg gacgagcgcg cgctagtgga tgcgatccgc cgccaggcgc
      781 gtcatgaaga gacgacgcgc tgggacacga tcgaattcaa gctcgactga ccggccggcc
      841 gctgcccgga cgggttgctc gcccgctttc agcctcggcg gcgcgcagcg agatcacctg
      901 cggaacgctc ttcagcatct tggcgcgcgt gcttgcggcc gtggaaccgc tcacgctcag
      961 ctcggaattc tccgtcacct ccgcgcgggc tccctcgcgc accattgcgg caaacaggcg
     1021 caccaccgtg caggcgagct catccgcctc gtgactggag agcttgcgca ccgtcgcacg
     1081 cacggcatct tcgccacgca acggtgcctc gcgcgtcaga gcgtcgtggc aggcacgaaa
     1141 ctccgcgcgc agaccctccg gcaactcctc aacgttcaca agcgacaggt gcttgcgata
     1201 tgcatcggcg aggcgatctt tgatggggcc ggaccgcacc agcgtcagcg cggcggcctc
     1261 aagctgttgc catgcagtgc tcatcacgac ctcggcgatg gcctcctgtt ggttctaggt
     1321 aggaggaggc ggcatcgatt ctttgttgtt ttctggtagg tactgaaacc ccagcgcgct
     1381 caatcggctg cagcgtaaat atagcgcagg ctctagcaaa tgaatatggc gagccgggtg
     1441 cctgaacggc acctgaaggc tcttcgagcc cactcggcgg cgccgccgcc ggacgtgcgg
     1501 tcgtcgcgcc ttcgtgccga tcgccacgca cgcgccctct gcaagcgcct gtggccatgc
     1561 cgcggcggat gtgcgcagct gcgcgagctg cctcgatccc gctcagggct cattgagccg
     1621 cggaaaaacc tgcctcgtga gcttggcgtg cgcgctcaag tgcggcttcg gccctcaaac
     1681 ggagaccagg tgaatcatcc tctataagtt attgatcttg ccgggctatt gtgcccagca
     1741 ctcctggctc gtgagagtgc cggtcgagaa ctgctgtccg gtcgagtcga ccgagaagga
     1801 ctggcactgc gtatcggcgg cctggctgct gccgccgacg ggagtcgcgg tgatgtagta
     1861 ggacggtccc ggcggcgcat tcggattcgg atcgcacgcg ggggttgcag ccgccgccgc
     1921 ggctgtgcac acggtgatcc tgtagtagtt atcaggcgcc accagcgccg ggaaggcgcc
     1981 tgcatatccc agatcggcgg gcgtcgtgga gtagagggcg ccgttggtgc tgaagtaacg
     2041 ctcctcacgg ccggcgagat ccagtaccgc ggtcttcgct tgcgtgcgcc gagattggcg
     2101 aacctggctc aggtaggagg gcacggcgat cgaaaccagg atcgtcgcga tcaccacgac
     2161 gatcatcagc tccatcaggg taaacccgcg ggacccgtgc gtaacggcgt gctgttgcat
     2221 gttctctctc gatgtccagc gacgcgacta gcgtcgctca atccagctca ggcgccggcc
     2281 ggtggtgttg ggcggcagat tcaggccgag cgtgccattg ccgatggtgc tgccgccgtt
     2341 ggtggagttg gtctcgaaca ccaggaacgc ggtgcccgcg ctgttgccgg tgacgaacga
     2401 gctgccggtg gcattcgtga gcatgccaac tgcgatcgag tcggtatagg cagggttgtt
     2461 gttaaccgtc ggattggccg cctcgctcgg gggcagaaat acctgctgga aggctccgcc
     2521 ggtcatggcg gacacgacgt aagtgaaacc ggtatccgag gtctgcgtgc aggagaccgg
     2581 attgttgggt gccggcacga tggagtttac ggtcagcgcc tgcgcgacca gctcggggtt
     2641 gaagataatc tgctcctgcg cgccggggag ggtgaggtac cacccgtact tgttgttatt
     2701 gcttgcacac gcgctgccgc ccgcccagca gatggtcgcg ttattggcta tttcccgatc
     2761 ctgggtcgcg gcgttcacgg tcacgagctg cgcctgcagg ttcgactggg cgatggtgta
     2821 gttgggactc gccaggttga cggtggctgc cggtgcaagg ctcgcatagt gcgcggagga
     2881 catggcgtcc caggcgctca tgttccagtc ccagacaccg tagagcgcct gcgtcccgga
     2941 ggcgtagctc gaggcgctgc tggtggtgag gggggtcttt tgacccgttc cgaacagcac
     3001 catcaccagc ggcgcggcac cgggggtcag tgaaccgccg gcgatggcga cctgcgtggt
     3061 aattggctgg ccggccggtg tggtgaacag cgggccgggc gtgaccgccc agttcgccgg
     3121 gctgttgctg gtgagatcaa agcgccacac gtgaccctgc agatcccccg catacacata
     3181 gtcggtgatg tggtcgccgt ccagatctgc cgcggatgcg tacgcgatgc cgttcggatt
     3241 cgccgcgctg ccggtagccg tactcagata gtagacagtc cgcgcggcgg tgttggggtc
     3301 gagcgtcatg atgtagatgc cggcatcgcc cgagacgctg ccgtaaccgt taccaaagat
     3361 gaccgcccag ttgccgttgt gcaggcgccg gatctgcggt gtgccgtagg tgttccccag
     3421 gttgttgccg cagttggtga cgttggtgca ggcgatggtc gaggaggtcc actcgcccac
     3481 caccaggctc gcagcgttgc cttcggtgaa gttcactgct gaagggttgg tcacatcgag
     3541 cgcgtaaatg gccgccccac cggctgcgag tccgcccacg agccaggtgt gccattgacc
     3601 gccgtagaag aggtcgccag aggttggagt ggcatcgacg aagaagttgt gggcgtactg
     3661 ggcgttcgca tagtcgacgc tgttggtcat cgagtggatg gtctgcagca ccgctccagg
     3721 catgtaggcg agcacctccg ccccatcgtt gggcgtcagc gcgttgttga cgaaattgcc
     3781 attcgcgtcg taggagccgc tgcgaaagcc gtgcaccagg ccgtcgttgg cgccgacgta
     3841 caccacgttg agccgcgtct gctctgcggt gacataagcg gcgtaggtct gcgcgccgtt
     3901 ggcgctctcg ggattgttcg cgcccgggta cagacggtcg ttccaggccg ccgtgtaagg
     3961 cgcagtcggc ggccccaccc aggtggggct ggaatcgacg atgtccgcga gcacatcggt
     4021 gcgggcgcgg aacaggccga cgcccgagga gttgatttca tcgctgcggc tgccgcgcag
     4081 atagcgctcg cgggcgaata ccgtgtagct ggg
//
LOCUS       DATDMP010000554         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1089137, whole genome shotgun sequence.
ACCESSION   DATDMP010000554 DATDMP010000000
VERSION     DATDMP010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089137"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1387)
                     /locus_tag="VI195_09730"
     CDS             complement(128..1387)
                     /locus_tag="VI195_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824709.1"
                     /translation="MPDERPPRALTAPRTLIMLLTLVLVISYIDRGNLATAGPLLTEE
                     LGLSLSQFGTLLSAFYFAYATLMIPAGWLAERHGARPVLAVAVGIWACATLATGFAGS
                     FASLLALRVLLGVGESAVFPAWSKLIRAGVDEAHAGIANGVVSFGYLVGPAIGTFAGG
                     LLMSRFGWRVTFWLFGALSLAWLVPWSRVRVQEATTRGGVGSAHVPSFATILRQRALW
                     GASIGNFGINYSFFFVLAWLPTYLVKERGFSMNEMAGTAAPAYALSAIGSLLAGFLID
                     RWIRDGRSANTIHKGLMAVGYATGIVAMIGIALLPRGGSIASLYFYEFFTGISSPPLY
                     AVSQTFAGPGGTARWVGVQNLCGNLAGIIASALTGVLVESMHSYLAAFAVAALANVIG
                     FCGWVLLMPRVAPIRWPARAAAPRPAT"
     gene            2216..>2412
                     /locus_tag="VI195_09735"
     CDS             2216..>2412
                     /locus_tag="VI195_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY6824710.1"
                     /translation="MNVCTRKSNHLFRLLALFAICFLGATIAWAAPAETRSMEVSFRD
                     LDLSTQSGAVKLYRRIHAAARG"
BASE COUNT          484 a          780 c          751 g          397 t
ORIGIN      
        1 gagagcaaat gcgaaaagcg ggacaatcca cacacgcatc tacggccccc ttttctggtc
       61 gcttattggc tcgattgacg cactctgatt attccgcaat cctccacgcc ggcctaatgc
      121 tgaggtgcta cgtggccggg cgcggtgcgg ctgcgcgtgc gggccaccgg atcggcgcga
      181 ccctcggcat caagagcacc caaccgcaga agccgatgac attggcgagc gcagctactg
      241 caaatgccgc gaggtacgaa tgcatgcttt cgacgagcac accggtgagt gccgaggcaa
      301 tgatcccggc caggttgccg cacaggttct gcacgccgac ccagcgggcc gtgccccccg
      361 gacccgcgaa cgtttgcgac acggcataga gcggcggcga ggagatgccg gtaaagaact
      421 catagaagta gaggcttgcg atcgagccac cgcggggcag caaggcgata ccgatcatgg
      481 cgacgatccc agtggcgtag ccgaccgcca ttaggccctt atgaatggtg ttcgccgagc
      541 gtccgtctcg gatccaccgg tcgatcagga aaccggccag tagcgaaccg atcgccgaca
      601 aggcgtacgc cggagccgcc gtgcccgcca tctcgttcat cgagaacccg cgctctttga
      661 cgaggtaggt cggcagccat gcgagcacga agaagaaact gtagttgatc ccgaagttgc
      721 cgatcgacgc accccacagc gcccgctgac ggaggattgt ggcaaaggac ggtacatgtg
      781 cggacccgac gcccccgcgt gtcgtcgcct cctgcactcg tacccggctc cacgggacga
      841 gccaggcgag cgatagcgcg ccgaacagcc agaaagtgac gcgccagccg aagcgcgaca
      901 tgagcagtcc cccggcgaac gtcccgatcg ccggtccgac caagtacccg aagctgacaa
      961 caccgttggc gatgccggcg tgcgcctcgt cgactcccgc gcggatgagc ttcgaccagg
     1021 ccgggaatac ggcagattcg ccgacgccga gcagcacccg caaagcgagc aacgaggcga
     1081 acgagcctgc gaagccggtc gcaagcgtgg cacacgccca gattccgacg gccacagcga
     1141 gcacggggcg cgcgccgtgg cgctcagcca gccagccggc cgggatcatc aaagtcgcat
     1201 aggcaaagta gaacgcggat aggagcgtgc cgaactgtga cagtgaaagt ccgagctcct
     1261 cggtcaacag cggcccggca gtcgcgagat tgccccggtc gatgtagctg atgacgagca
     1321 ccagcgtcaa cagcatgatc agcgtccgcg gcgccgtcag cgcgcggggc ggccgttcgt
     1381 ctggcatcct cgtctcctgt gaggatgcta cgcgaccctc cgccagagcg ccatgcccaa
     1441 tggcggcagc gccgtcaccg tctcacgatc tcgcggactc cgtgttagaa cggctgagag
     1501 cgccgctcac tggtatgtct cgattttcag cgccggtatc ccaaccgata tgctgctaat
     1561 gatcttcgat atgtttacgc aggtggagcg cagcatggag caggtgcaga gtggaagatt
     1621 tggcgagccg attcgggtaa ggggcccgtg ctcgtggcga tcacgcatgc actttcccgg
     1681 cgagtgtggg cccgagcgcg ctaatccgac cggcgcgccc gactagtatc ggcgaaagga
     1741 taacgacggg cttccttcag caccgcttcg agtgcctgcc ggagcagata ctgcatggaa
     1801 aacccggttc gcttactcgt tgcgcgaagc aaccagtact gctcgggcgg taagtacagg
     1861 ctgatctgaa actgcttacc cttcagtgtc atgtagccgc cgcgtccgcg cctgagcgca
     1921 tcttacgcgt aagacgccgg cgagtggaaa tgacgctggg cgagtgatgc ggctggcggc
     1981 attgggttgc agcggtgagt tgcgatccaa gcccgagcca cacacccgga ctgctgcggc
     2041 gagcctagcc tcaccctcat taccttggtg gcttctgtgg cgagcgatcg ccgagcttca
     2101 ccaagcttca ccagtcttca cccacattca ccaagcttgg gcccagtttc atgcaccgcc
     2161 gcggcggggc gtatagcgtc tccgccgtgc ttcaaaaccc aagtgggaac cgcaagtgaa
     2221 cgtctgcaca cgcaaatcga accatctgtt tcgcttgctc gcgctatttg cgatctgctt
     2281 cctcggcgcc accattgcgt gggcagctcc ggccgagacc cgaagtatgg aggtcagttt
     2341 ccgcgacctc gacctgagca cgcagtccgg ggcagtcaag ctctatcgcc gcatccacgc
     2401 ggctgctcgc gg
//
LOCUS       DATDMP010000555         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_311241, whole genome shotgun sequence.
ACCESSION   DATDMP010000555 DATDMP010000000
VERSION     DATDMP010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311241"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VI195_09740"
     CDS             <1..348
                     /locus_tag="VI195_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY6824711.1"
                     /translation="VDIALVLLIIFMVTASYIVKETIEVELPRAAHGGETVQKTFAVL
                     VTKEGKAYLNGVAVDDAALISEVRKSKAAGEDVQAIVGADKNATHGAVTHLLDVLKGA
                     GVTKFAIQIEKQQ"
     gene            352..1116
                     /locus_tag="VI195_09745"
     CDS             352..1116
                     /locus_tag="VI195_09745"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY6824712.1"
                     /translation="MARAATRAVVPLGTSLLFHAALAAFLLAVAVRPRPVPQVPLQIQ
                     VVDVPPNPPPEPAKPAEPPPPVPMKVARAPRAAPKNLPPPTPMPLPQSSAPPPPTTEA
                     PQTSKEPVVITGITLESTSQGGSMAVGTGNTLRGTPERTARAPETVKPYKADKYASAA
                     QVSELPQPLNRESLNLRKYYPAQAKKDGFEGDVVLRLLIDADGTIAKVDVITDPGQGL
                     GPAAAQAVRELRFSPAKVNGVPVATTVPFTIHFTLD"
     gene            2012..>2320
                     /locus_tag="VI195_09750"
     CDS             2012..>2320
                     /locus_tag="VI195_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824713.1"
                     /translation="MWRELGGLSALAALLEWVRRRRFRDLLEESDEVFLSGVLRQLPT
                     AKPGDVLRERGWIAVLSGLPRKKVAYGLRLADLIERADAGLALQPGDLLEDLDRRLGV
                     V"
BASE COUNT          409 a          747 c          778 g          386 t
ORIGIN      
        1 gtggacatcg cgctcgtgct cctgatcatc ttcatggtca ccgccagcta catcgtgaag
       61 gagaccatcg aggtggagct tccccgcgcc gcgcacggcg gcgagacggt gcagaagacc
      121 ttcgcggtac tggtgacgaa ggagggcaag gcgtatctca acggcgtggc ggtggacgac
      181 gcggcgctga tcagcgaggt gcggaagtcg aaggccgcgg gcgaggacgt gcaggccatc
      241 gtcggcgcgg acaagaacgc aacccacggc gcggtcacgc acctgctcga cgtgctcaag
      301 ggcgcgggcg tgaccaagtt cgccatccag atcgagaagc agcagtaggc catggcgcgc
      361 gccgcgacga gagcggtcgt tcccctcggg acctcgctcc tcttccacgc ggcgctggcg
      421 gcgttcctgc ttgcggtcgc ggtgaggccc cgcccggtgc cgcaggtgcc gctgcagatc
      481 caggtggtgg acgtgccgcc gaacccgccg cccgaaccgg ccaagcccgc cgagccgccg
      541 ccgcccgtgc cgatgaaggt ggctcgcgcg ccgcgcgccg cgccgaagaa tctgccgcca
      601 cccacgccga tgcccctgcc gcagagcagc gctccgccgc cgcccaccac ggaagcgccg
      661 cagaccagca aggagccggt ggtgatcacc ggaattactc tggagagcac ctcgcaaggc
      721 ggctcgatgg cggtgggcac cggcaacacg ctgcgcggaa cgccggagcg cacggcccgc
      781 gctccggaga cggtgaagcc gtacaaggcc gacaagtacg cctccgcggc gcaggtgagc
      841 gagctacccc agccgctcaa tcgcgaatcg ctcaatctac gaaaatacta tccggcccag
      901 gcaaagaagg acggcttcga gggcgacgtg gtgctgcgat tgctcatcga cgccgacggc
      961 accatcgcca aggtggacgt gatcaccgac ccgggccagg gcctgggccc cgccgccgcc
     1021 caggccgtcc gcgagctgcg cttctcgccg gcaaaggtca acggcgtccc ggtcgccacc
     1081 accgtgcctt tcaccatcca cttcacgctc gactgatcgc caatacatct gaaactcagg
     1141 attatttggg ctccgaaggg atctcatggc cgttttgcaa cgcattaggt cttgactgaa
     1201 tcaacgattc ctgcgactcg accgcttgac tcgcgccgag ggggcggaat gggccgcgct
     1261 gccgttgctg tattcctggt gtcggtcgcc ggctggagcg gcgccgctcg cgctggcgga
     1321 tgtcctctga cgggcaagcc ggactgcggt gcgtgcggac agctcacgtg cgatccagag
     1381 ggcttctggt actgcgattc cgctcccaaa gacgggctcg cttgcgacga cgggaatctc
     1441 tgcccctggg gcgaccactg cagcctgggc acctgcgtcg gaaccgcgat tacctgtgcc
     1501 gatacggcgt gctctacgca gacgtgcaac ggcacgtcgc agtgctccat cgtacccgag
     1561 ccgagcagca cctgttgtac gcggacctcg ggtcgcgcag tcgggatcaa ggccatgtcg
     1621 gaagggagca cagtggggac cgggcgggcg gcagcaagag cggccgaccg cgaacagtga
     1681 tcaccaggaa tatcgattat ggggattgcc tcgccggctg cattgaggag aaccgactcg
     1741 acagcctgtt gcctctgctg ttcagtgctt ggccaaaagc gatgctgcca ccctttcgcg
     1801 ttccatatcc gagtcaaccc ttgactaccc cattaagctc actcggtcac ctcctgggcg
     1861 aggccggcgc ccctttcgag ctgactcgat tgttgcgcga tatcggcaga ggggcctcgg
     1921 gaatcggtac ggctgcgctc gtattcgagg gtttctacga tatcggtacg atcggtcggt
     1981 gtgcggtcgt atgcaattag gtgaggagaa catgtggcgt gagctaggcg gactgtcggc
     2041 gctcgcagca ctcctggaat gggtgcggcg tcggcgcttt cgggatcttc ttgaggagtc
     2101 tgacgaagta tttctcagcg gtgtccttcg tcagctgccg acggccaaac ccggggacgt
     2161 gctgagggag cgcggatgga tagccgtcct cagcgggctc ccccgaaaga aagtagcata
     2221 cgggctgagg ctcgcggatc tcatcgaacg ggcggatgct ggtctggcgc tccagccagg
     2281 tgacttactg gaggatctcg atcggcggct cggagttgta
//
LOCUS       DATDMP010000556         2697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1865993, whole genome shotgun sequence.
ACCESSION   DATDMP010000556 DATDMP010000000
VERSION     DATDMP010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865993"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VI195_09755"
     CDS             complement(<1..265)
                     /locus_tag="VI195_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824714.1"
                     /translation="MKRSARALEEALGQLDSGLLRRRRTTVEGFAQPGNRLLALAGGR
                     TLVDFSSNDYLGLARHPALAAAMGGCAREAGAGSAASHLVTGHG"
     gene            complement(240..1271)
                     /gene="bioB"
                     /locus_tag="VI195_09760"
     CDS             complement(240..1271)
                     /gene="bioB"
                     /locus_tag="VI195_09760"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY6824715.1"
                     /translation="MSDAHPDAIRHDWTLEEVEALFALPFMDLLYRAQRVHRAHHAPN
                     TVQLSTLLSIKTGACPEDCAYCPQSVHYDTGLRREALMEVAAVRECAERARHAGATRF
                     CMGAGWRSPRPRDLAVVAEMIRAVRALGLETCATLGMLTPAQAAQLKTAGLDYYNHNL
                     DTSPEFYGEIITTRTYQDRLDTLAAVRAAGLKVCCGGIVGMGESTTDRAALLRTLANL
                     PEHPGSVPINRLVRVPGTPLADAPEVDPFDFVRTCAVARVLMPRAHVRLSAGRDTMSD
                     ELQALCLLAGANSIFYGERLLTTGNPEVERDRQLLARLKVTAEEAAAAAPAPGDAAAG
                     DHEAQRARA"
     gene            complement(1396..1764)
                     /locus_tag="VI195_09765"
     CDS             complement(1396..1764)
                     /locus_tag="VI195_09765"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY6824716.1"
                     /translation="MGGSWKNRAFSARLGFALRGLAQALRSEQSLRLQALALAATLLA
                     LLFLHPGALWWALVLLASAAVIAAELLNTAIERLADELHPHDGPGIGIVKDCAAAAVL
                     IAVLGALAVALALLLHLLHR"
     gene            complement(1783..>2697)
                     /gene="ubiA"
                     /locus_tag="VI195_09770"
     CDS             complement(1783..>2697)
                     /gene="ubiA"
                     /locus_tag="VI195_09770"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY6824717.1"
                     /translation="PEPLSTPTLPEAPFSYRLGRRLGGYGRLMRLDRPVGTWLLLWPA
                     LWALWIAGAGRPRPRVLIVFVAGVVVMRAAGCVINDFADRDIDPYVRRTRDRPLAARH
                     VSPVEALVLFAVLIAVAAWLVTRLDRFTVELACIGALLTVSYPFVKRAFPLPQLYLGI
                     SFGGWSVLMAYAAQAGALPRVAWVLYIAAVIWASVYDTIYAMIDREDDIRIGVKSSAI
                     LFADMDRLLIGVMQAMMLYALYLVGIDMHFGRWYYAGIAGAAALFAWQQWLIRRRHPG
                     GCLRAFLNNQYVGLVLFVGILLQYVYAR"
BASE COUNT          421 a          939 c          995 g          342 t
ORIGIN      
        1 cgccgtggcc ggtgacgagg tgcgaggcgg cgctgcccgc gccggcctcg cgggcacacc
       61 cgcccatggc ggctgcgagc gccgggtggc gcgcgagccc caggtagtcg ttgctgctga
      121 agtcgacgag cgtccgcccg ccggcgaggg cgaggaggcg gttgccgggc tgcgcgaagc
      181 cctcgaccgt ggtacgccgg cgccgcagca ggcccgagtc gagctgaccg agcgcctcct
      241 caagcgcgcg cgcgctgcgc ttcatggtcg ccggcggctg catcgcccgg cgcgggcgcg
      301 gccgcagcgg cctcctccgc cgtcaccttg agccgggcga gcagctgccg gtcgcgctcg
      361 acctcggggt tacccgtggt gaggagccgc tcgccgtaaa agatcgagtt ggcaccggcg
      421 agcaggcaca gcgcctgcag ctcatccgac atggtgtcgc gcccggccga gagccgcacg
      481 tgggcgcgtg gcatcagaac gcgcgccact gcacaggtgc gtacgaagtc gaaggggtcg
      541 acctcgggcg catcggcgag cggcgtgccc ggtacccgca ccaggcggtt gatgggcacg
      601 ctgccgggat gctcgggcag gttggcgagc gtgcgcagca gtgcggcgcg gtcggtggtc
      661 gattcgccca tgccgacgat gcccccgcag cacaccttga gcccggcggc gcgcaccgcc
      721 gcgagcgtgt cgagccggtc ctggtaggtg cgggtggtga tgatctcgcc gtagaactcg
      781 ggcgaggtgt cgaggttgtg gttgtagtaa tcgaggcccg ccgtcttgag ctgggctgcc
      841 tgcgccgggg tgagcatgcc gagcgtcgcg caggtctcga gccccagcgc gcgtaccgcg
      901 cggatcatct ccgcgaccac cgccaggtcg cgcgggcgcg gcgagcgcca gccggcaccc
      961 atgcagaagc gcgtggcccc ggcgtgccgg gcgcgctcgg cgcattcgcg caccgctgcc
     1021 acctccatca gcgcctcgcg cctgagcccg gtgtcgtagt gcacgctctg cgggcagtag
     1081 gcgcaatcct cggggcaggc gccggtcttg atcgacagca gcgtcgagag ctgcacggtg
     1141 ttgggcgcgt ggtgcgcgcg gtgcacgcgc tgggcgcggt agagcagatc catgaacggc
     1201 agcgcgaaca gcgcctcgac ttcctcgagc gtccagtcgt ggcggatggc gtcggggtga
     1261 gcgtcgctca tggcggcgac gatgcccgag ggcccgccct cgtgtcaacg cggcggcctg
     1321 cgccaagtcg acaatcgtgt ccccggcaac gaaggcgatg tccgaaaaat cgccgcaggc
     1381 gatttttcca atggactagc ggtgcagcag atgcaggagg agcgcgagcg ccaccgcgag
     1441 cgcgccgagc acggcgatca gcactgccgc ggcggcgcag tccttgacga tgccgatccc
     1501 ggggccgtcg tgcgggtgca gctcgtcggc gaggcgctcg atggcggtgt tgaggagctc
     1561 ggcggcgatc accgcggcgc tcgcgagcag taccagcgcc caccacagcg ccccgggatg
     1621 caggaaaagc agcgcgagca gcgtcgcggc gagcgccagc gcctggagcc gcaggctctg
     1681 ctcgctgcgc agcgcctgcg cgagcccgcg cagcgcaaac ccgaggcgcg cactgaacgc
     1741 ccggttcttc cagctcccgc ccatgtgccc ggcccggccg cttcagcgcg cgtacacgta
     1801 ctgcagcagg atgccgacga acagcaccag gccgacgtac tgattgttga ggaaagcgcg
     1861 caggcacccc cccggatggc ggcggcgaat gagccactgc tgccaggcga acagcgccgc
     1921 tgcgccggcg atgcccgcgt agtaccagcg cccgaagtgc atgtcgatgc ccaccaggta
     1981 gagcgcgtac agcatcatcg cctgcatgac gccgatgagg aggcgatcca tgtcggcgaa
     2041 caggatggcg ctcgacttga cgccgatgcg gatgtcgtct tcgcggtcga tcatggcgta
     2101 gatcgtgtcg tagaccgacg cccagatcac cgcggcgatg tacagcaccc acgcgacccg
     2161 tggcagcgct cccgcctggg ccgcgtaggc catcagcacg ctccagccgc cgaaggagat
     2221 gccgaggtac agctgcggca acggaaatgc ccgcttgacg aacgggtagg agaccgtgag
     2281 cagcgccccg atgcaggcga gctcgacggt gaacctgtcg agacgcgtca ccagccacgc
     2341 cgcgaccgcg atcagcacgg cgaacagcac cagcgcctcg accggcgaga cgtgccgcgc
     2401 ggcgagcggc cggtcgcgcg tgcggcgcac gtacgggtcg atgtcgcggt cggcgaaatc
     2461 gttgatcacg cagccggcgg cgcgcatcac caccacgccc gccacgaaca cgatcagcac
     2521 cctgggtctg ggccggccgg ctcccgctat ccacagcgcc cacagcgccg gccacagcag
     2581 cagccaggtg ccgaccgggc ggtcgagccg catgagacgc ccgtagccgc cgagccgccg
     2641 gccgaggcgg tacgagaacg gtgcctcggg cagggtcgga gtcgacaggg gttcggg
//
LOCUS       DATDMP010000557         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1866286, whole genome shotgun sequence.
ACCESSION   DATDMP010000557 DATDMP010000000
VERSION     DATDMP010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866286"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1559)
                     /locus_tag="VI195_09775"
     CDS             complement(120..1559)
                     /locus_tag="VI195_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY6824718.1"
                     /translation="MSGQSRNAADEAASSAPIRTVNPATGERGQTYEPHSLDEARSIA
                     ASCAAAQKLWRKTPIAERSELMHTAAQLMRANKSRYAALMTEEMGKTVTEGLAEVEKC
                     AATADYFAENAAAFLAPRPQTWNDGAAGSTATRAFVTFNPLGVVLAVMPWNFPFWQAM
                     RFCAPHLMAGNGGVLKHASNVPGCALALQEIFADAGFPRELFRTVLVGSQQVKALIED
                     PSIAAVTLTGSVNAGKAVAAAAGAVLKKTVLELGGSDGYIVLADADVPKAAQVCAAAR
                     MINAGQSCIAGKRFVVLEPVRAAFEEALVEAMRAYVFGDPRDPATRLGPLQSVKARDE
                     IHEQVKRSVAGGARVLLGGEVPQRPGAWYPATVLTDVTEGQPAHDEEVFGPVAAIISA
                     RSEADAIRIVNGTQFGLGSGVLTRDLARGERIAAEELDAGLAFVNQYVRSDARLPFGG
                     VKQSGYGRELSEFGVYEFCNIKTVLVERM"
     gene            1722..2396
                     /locus_tag="VI195_09780"
     CDS             1722..2396
                     /locus_tag="VI195_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY6824719.1"
                     /translation="MDKTSRAKPEGKAAVAPLLAKPVHKIPEGGLVALRRKARQCHAC
                     GLWKHATQTVFGAGADDARIMLIGEQPGMQEDIQGLPFVGPAGRLLDRALEEAHLDRD
                     TLYLTNTVKHFKYELRGKLRLHKRANAAEQEACRMWLAAELARLQPRVVVALGAMAAQ
                     TVFGSSFTLTRERGQWRRLGPATEGFATFHPSAILRTPDEKRAAMYRQLVEDLSEITK
                     RTELHE"
BASE COUNT          452 a          804 c          764 g          427 t
ORIGIN      
        1 cggtaacagg ggttcgaatc cccttgggga cgccaatcac cccaatgatt gagccatcac
       61 agatttcgaa ccggaagtga ctctgctcgg ttagggttga tgacctgtcc gcatccccat
      121 cacatacgct cgaccagcac agtcttgata ttgcagaact cgtaaactcc gaactccgac
      181 aattcgcgcc cataaccgct ttgcttcacg ccgccaaacg gcaggcgcgc atcggaacgc
      241 acgtactgat tgacgaaggc gagcccggcg tcgagttcct cagccgcgat gcgctcgcca
      301 cgcgcaagat cgcgcgtcag tacaccgctg ccgaggccaa actgcgtgcc attcacgatg
      361 cggatagcat ccgcttcgct acgtgcgctg atgatggccg ccaccgggcc gaagacctct
      421 tcgtcgtgcg caggctgacc ctccgtcacg tcggtgagga ccgtggcggg gtaccacgct
      481 ccgggtcgct gcggcacttc acctccgagc agcacccgcg ccccgccggc cacactgcgc
      541 ttgacctgct cgtgaatctc atcgcgcgcc ttaacactct gcagcggtcc gagtcgggtg
      601 gccgggtccc ggggatcacc gaacacgtag gcacgcatgg cttcgacgag ggcttcctcg
      661 aaggccgcgc gcaccggctc gagcaccacg aaacgcttgc cggcgatgca gctctgcccg
      721 gcgttgatca tgcgggccgc ggcacaaacc tgcgccgctt tgggaacgtc ggcatccgcc
      781 agcacgatat aaccgtccga gcctccgagc tcgagtacgg ttttcttcag aaccgctccg
      841 gccgccgctg cgacggcctt gccggcattg acgcttcccg tcagggtgac agcggcaatg
      901 ctcgggtctt cgatcaatgc cttcacctgc tgcgagccca caagaacggt acgaaatagt
      961 tcacgcggaa agcccgcatc cgcgaagatt tcttgcagcg cgagcgcgca ccccggaaca
     1021 ttcgaggcgt gtttgagcac acctccatta ccggccatca ggtgcggcgc gcaaaaccgc
     1081 atcgcctgcc agaaggggaa gttccagggc atgacggcga gcaccacgcc gagtggattg
     1141 aaagtcacga acgccctggt ggcggtgctg ccggcggcgc cgtcattcca cgtctgtggg
     1201 cgcggtgcga ggaacgcggc ggcgttttca gcgaagtagt ccgccgtcgc ggcgcacttt
     1261 tcgacctccg cgagtccctc ggtaactgtc ttgcccatct cctcggtcat gagggctgca
     1321 tagcgggact tgttggcccg catcagctgc gcagccgtgt gcatgagctc actgcgctcg
     1381 gcgatcggcg ttttgcgcca tagcttctgg gccgccgcac aacttgcggc aatgctcctc
     1441 gcctcatcga gcgaatgcgg ctcataggtt tggccacgct caccggtggc ggggttgacg
     1501 gttcggatag gggcgctgct cgcggcttcg tcggcggcgt tgcgggactg gcctgacatg
     1561 accggctcct tgactgaaca atggcgcaat ctcttttgcg gacgatagcg cataatcgcc
     1621 agcactgcgg cgttggggac caatgcgctg cacactggcc aagttcaagc aggatgctgc
     1681 aaggccgggc aagtgcacac cgccacgcaa ggcctgaagt catggacaag acatcgcgtg
     1741 cgaagcccga aggtaaggcg gcggtcgcgc ccctgctcgc caagccggtg cacaagatcc
     1801 ccgaaggcgg cctcgtggcg ctgcggcgca aagcacgcca gtgccacgcc tgcggactct
     1861 ggaagcatgc cacgcagacg gtattcgggg ccggagccga tgatgctcgc atcatgctga
     1921 tcggcgaaca gccgggtatg caggaagaca tccagggact gccgttcgtc gggcccgcgg
     1981 ggcgtctgct cgatcgtgca ctcgaggaag cacacttgga ccgcgacacg ctctatctca
     2041 ccaatacggt gaagcacttc aagtatgagc tgcgcggcaa gttgagattg cacaagcgcg
     2101 ccaatgcggc ggagcaggag gcgtgccgca tgtggctggc agcggagctc gcccggctgc
     2161 agccccgcgt cgtggtggcg ctcggggcga tggcggcaca gactgttttc ggcagcagtt
     2221 tcaccctcac acgcgagcgc ggccagtggc gccgcctggg ccctgctacc gagggctttg
     2281 ccacttttca tccgtccgca atactgcgca ctcctgatga gaagcgcgca gccatgtatc
     2341 ggcagctcgt ggaggacctg agtgagatta ccaagcgcac ggagctccac gaatgaagct
     2401 ggcggccacg ccgcatccgg cgacctcaac tcgctctgac gggaatc
//
LOCUS       DATDMP010000558         7954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1644332, whole genome shotgun sequence.
ACCESSION   DATDMP010000558 DATDMP010000000
VERSION     DATDMP010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644332"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VI195_09785"
     CDS             <1..1111
                     /locus_tag="VI195_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6824720.1"
                     /translation="AASSRGRAVNNTIHLSDLLAARRTEILARWAQRITREHEDKELS
                     RGELWDHLPRLFDEVLQALREEGPFSANAVGPESKAASAAHGAQRLRVGFDLTEVGRE
                     YEILTECILDEVEAVGGTVSIQAFRRLQALLNEGRRYAVSAYIDRRDTEIGRQHSQHI
                     AFIAHELRNPLMTASMAATALRRNVQSEDTRVLDMLVRNLTALRELIDQVLIADRLIA
                     SMQIKRESLDLRVLLDEVITDSRLAAERRQIEFKMEVPDRLLCSGDQRLLRSAIGNLL
                     ANAVKFSHAGQPITLRAVLRDNGVTIEVEDRCGGLPDSNIADLFQPFVQKGENRTGFG
                     LGLAIVKQAAEAHGGKVNVRNLPGQGCVFSLELPA"
     gene            complement(1586..1897)
                     /locus_tag="VI195_09790"
     CDS             complement(1586..1897)
                     /locus_tag="VI195_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824721.1"
                     /translation="MDQDSVKLGLLIEAAQAHQKLAEAAVEKLRERTQALEPVVRDQV
                     QRALADELNSLRTESIRVIEALQTAKRGQCAHRLLDAWCHGDHHGDRALRCVVGAPDA
                     G"
     gene            1986..2447
                     /locus_tag="VI195_09795"
     CDS             1986..2447
                     /locus_tag="VI195_09795"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEY6824722.1"
                     /translation="MAPLESPALADFVANLDRINALAEGSAGFVWRLKSDSGNATDIK
                     VSEDLRFIINMSVWSSIEALFEFVYRTAHTGIVARRREWFERPIEAYQVLWWVPAGHK
                     PPPQEALERLELLRRRGPSAEAFSFKQRYPAPGEAGDPSDMQPEPSCVGWT"
     gene            complement(2939..3409)
                     /locus_tag="VI195_09800"
     CDS             complement(2939..3409)
                     /locus_tag="VI195_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824723.1"
                     /translation="MSDAMSWDGNWAWALPLIVLTLVLHVVGLALINLRMVRFIRRIR
                     AGRKFFPIFVSVMGVSALLAILLLGFEAALWAATFRSLGALPDGRTAMLYSLNAITSY
                     GHTDLLLAPHWRLMGALEALNGMLLFGLTTAFLYGHIRQVWPAELQPVAHDLNA"
     gene            complement(3510..3585)
                     /locus_tag="VI195_09805"
     tRNA            complement(3510..3585)
                     /locus_tag="VI195_09805"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3550..3552),aa:Gly,seq:gcc)
     gene            complement(3711..4265)
                     /gene="pgsA"
                     /locus_tag="VI195_09810"
     CDS             complement(3711..4265)
                     /gene="pgsA"
                     /locus_tag="VI195_09810"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY6824724.1"
                     /translation="MAWNLPNTLTWTRIAAIPLIILLFYMPYPWADPAAGLLFAAAGV
                     TDSLDGYFARRLGQISRLGAFLDPVADKLIVAVALVLLVSKDPRALIVLTAIVIIGRE
                     ITISALREWMAEIGARRKVAVSQLGKYKTILQITGLSMMLYRWPLLGLPTYRVGVALT
                     VIAAAATLVSMLVYLRAAWPELKR"
     gene            complement(4275..6086)
                     /gene="uvrC"
                     /locus_tag="VI195_09815"
     CDS             complement(4275..6086)
                     /gene="uvrC"
                     /locus_tag="VI195_09815"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY6824725.1"
                     /translation="MFDHKEYIASLPRRPGVYRMYGAAHELLYVGKARSLKDRVASYF
                     APRNLDPKVQALISQISAIEVTVTNSETEALLLEYNLIKAHKPRFNVVLRDDKSFPYI
                     HLYIEHDYPRLEFYRGARSAPGRYFGPFPSAGAVRDTLNQLQKLFRIRNCRDSFFANR
                     SRPCLQHQIGRCSAPCVGLITREAYVQDIAAAVKVLEGRSEEVSAELQARMEQAGARL
                     EYERAAEIRDQLAALKRIQAQQVVMAEGARDADVFAIAGEAGEYAVSVMLVRGGRNLG
                     TSSYFPRAALAEPHEALTSFVMQYYANADAPGEILLGSRTEEAPALAEALRERLGRAL
                     EVRCPSRGIGARWVELTAENAAQALRMRFAHRAGMDEMLADLARELDLPEPPQRLECF
                     DISHTGGEGTVAACVVFGPEGPLKKEYRRFNIRGVTPGDDYAALRQALERRYRRVGEG
                     EVPAPDVLLIDGGLGQIREVHEVLAALGFAELALVGVAKGPERRAGQERLFVYGAQSP
                     IMPVAHGPASRFVQRIRDEAHRFAISGHRKKRARRYNQSVLEVVPGLGPAKRRALLKH
                     FGGLQGIMRAGVADLTQVSGIGAALARTLYDHLHPGS"
     gene            complement(6090..6782)
                     /locus_tag="VI195_09820"
     CDS             complement(6090..6782)
                     /locus_tag="VI195_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY6824726.1"
                     /translation="MEPPRPEAGRAYDPDCTRCPRLAEFLEHIRQRHPGYWARPVPSF
                     GAARPRIVIVGLAPGLHGANRTGRPFTGDHAGVLLYRTLYAAGLASRPQSHSAEDGLR
                     LIQVRIINTVKCVPPANKPLPEEVRRCNGYLAAELQQLRSVRVYLALGRVAHEGLLLA
                     LARRRTQYPFAHGREHQLDGTRWLIDSYHCSRYNTATRRLTAEMFEQVVARACQRAQL
                     PGRRARTGAAAD"
     gene            complement(6763..>7954)
                     /locus_tag="VI195_09825"
     CDS             complement(6763..>7954)
                     /locus_tag="VI195_09825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY6824727.1"
                     /translation="FLLQGGDCAETFADCESDVIAKKLKILLQMSLVLVHGLKKPIVR
                     VGRMAGQYAKPRSADTETRGEATLPSYRGDLVNRPEFTAAARAADPELLLRGYERAAL
                     TLNFVRALVEGGFADLHHPEYWDLGFVHQAPLKDAYQRIVRSIGDALDFFEGMSGHPV
                     HEATLVDFYASHEGLHLLYEQAQTRFIPRQNRWYNLSTHMPWIGIRTAALDCAHVEYF
                     RGIANPIGVKIGSAMDAAWLQGLVHTLNPQNQPGRLTFIHRFGAKEVAAALPKAIEAV
                     RQTGQTVLWVCDPMHGNTETTTGGLKTRRFENILRELDLSFRLHDELGSHLGGVHIEL
                     TGDDVTECTGGARGLTDADLARAYRSSVDPRLNHEQALELAMLIAERSQSRRSERRSG
                     AAAP"
BASE COUNT         1450 a         2553 c         2554 g         1397 t
ORIGIN      
        1 ggcggcgtcc tcaagggggc gcgcggtgaa caacaccatt catctcagcg acctgctcgc
       61 tgctcgccgc acagagatcc tcgcgcgctg ggcgcagcga atcaccaggg aacacgaaga
      121 caaggaattg agtcgcggcg agctttggga tcatctaccg cgccttttcg acgaggtgct
      181 tcaagcactg cgcgaggagg gaccattcag cgcaaatgcg gtggggcccg agagtaaagc
      241 cgcctccgcc gcgcacggcg cccagcgcct gagagtggga tttgatctga cggaggtggg
      301 tcgggaatat gaaatcctga ctgagtgcat tctcgacgag gtggaagcgg tcggcggtac
      361 cgtgtcgatc caggcttttc ggcggttgca ggctctgctc aatgagggcc ggcgttatgc
      421 ggtttcagcg tacatcgatc gtcgtgacac cgaaatcggt cgccagcact cgcagcacat
      481 cgccttcatc gcacatgagc tgcgcaatcc gctgatgacc gcatccatgg ccgccacggc
      541 gctacgtagg aatgtgcagt cagaggatac gcgggtcctg gatatgttgg tccgcaatct
      601 gaccgcgcta cgtgagctca ttgaccaggt actcatcgcc gatcggctga tagcaagtat
      661 gcagattaag cgcgaatcgc tcgatctgcg ggtactactc gatgaagtga tcacagacag
      721 ccgcttggcc gcggagcgtc gtcagatcga gttcaaaatg gaggtgcccg acaggctgct
      781 ctgtagtggc gatcaacgat tattgcggtc cgccatcggc aacttactcg cgaacgcggt
      841 caagtttagc cacgcgggcc agccgataac cctgcgagct gtgcttcgag acaacggcgt
      901 cacgatcgag gttgaggacc gttgcggcgg tctccctgac agcaacatcg cggatctgtt
      961 ccagccgttt gtgcaaaagg gtgagaaccg cacgggcttc ggattgggac tcgcgatcgt
     1021 caaacaagcc gccgaggcgc acggcggcaa ggtgaacgtc cgcaacttgc ccgggcaagg
     1081 ctgcgtattc tccctggagc tgcccgcata ataagcgact gcacaggttg cgtggattcg
     1141 cgagcaacac gcaccggtct tctaatcgca atcaaaacgg aaaccgttag ccgaatggct
     1201 tgacggagac agtcgtgcat gcaactcgcg ctcgcgactt cctgcgataa cgctcgagtg
     1261 ccacttgaat tagaagcgat ccttgggtgt tgatgcgcgg ccggaggcga agattcacgc
     1321 cggccgtaca ggcaatcata cccgcgtcct gcgctgtcga gggtgagagc atcggctcag
     1381 ctcatcggtg ctcacagctt tcttgcgtca atatccccga atcatcaggt agtcggaact
     1441 ctcgccgtac cttggccctt tgaggtccac tcgaacacac aggtgcccgg tgccgcagcg
     1501 acgaagatcc gccgccgcac cccgttgatt gagggccgca atcttgctcg caagctcgtc
     1561 acgctcggtg cgcaaagcca tgatctcagc cggcgtcggg agcacccacc acgcaacgaa
     1621 gagcgcgatc gccgtggtgg tcgccgtgac accaagcgtc cagaaggcgg tgcgcgcatt
     1681 ggccgcgctt tgccgtttgc agagcctcga tcacacgtat gctctccgtg cgcaggctgt
     1741 tcagctcgtc agcgagtgcg cgctggacct gatctcgcac caccggctcc agcgcctgag
     1801 tacgctccct cagtttctcg acggccgctt cagcgagctt ctggtgcgct tgggcggcct
     1861 ctatcaggag gccgagcttt acgctgtctt ggtccatggg atgcaccgga ctttgcgcaa
     1921 ggtgcaggga tggaccctat cgggtcctgt cctgactacg tgcccggagt cgcgaccgct
     1981 cgaccgtggc gccgctcgag agcccagcgc tcgcagattt cgttgccaac ctcgaccgca
     2041 tcaacgccct cgccgaaggc tccgccggat tcgtctggcg tctgaaatcc gacagtggca
     2101 acgctaccga tatcaaagtc tctgaggatt tgagattcat catcaacatg tcggtatggt
     2161 cctcgatcga ggcgctgttc gaattcgtct atcgcacggc gcacacgggc atcgtggcgc
     2221 ggcggcgcga atggttcgag cggccaatcg aagcctacca ggtgctctgg tgggtgcccg
     2281 caggccacaa accgcctcca caggaggcgc tcgagcgcct cgagctgctg cgccgccggg
     2341 gaccgagcgc tgaggcgttc agtttcaagc agcgctatcc ggcgcccggc gaggccggtg
     2401 atccctcgga tatgcagcct gaaccctcct gcgtcggctg gacataagag cttgtcatcc
     2461 acggccggct agtctggggc gtgcgggact tttcgcagtc atcacgcccg gcgtgtccgg
     2521 tgacggtaaa aatcggggag ccccgtcaat gactcttcat ggccgttcgc gctgttctac
     2581 cacccagtcc agatcgactt atcggaggcg ctccctgggt cccacagtct cggggatgct
     2641 cagcagccgg cgagcacgac catggaagcg agacctggca cgcgacgcag ctggaagcgc
     2701 tcccgtgcct ccaatcgtcg cttaatctct gcgactagag agccacaggt gcgacgtata
     2761 tattgcgcag cccgcggggc tgcaaggaag ccggtggcgt ccataggctt ccagcagcag
     2821 ctgtgccccc cgaagggggt aaatatcggg ggaattgtgc tcgtgaactt cacgaagcac
     2881 catgcaaata ggcatttctg cgcgcggttc gactcctgct gccttccgcc acttgccact
     2941 aagcattcag gtcgtgcgcc accggctgca attcggcggg ccatacctgg cggatatgcc
     3001 cgtatagaaa tgcggtggtg agaccgaaga gcagcatgcc gttcagtgcc tcgagcgcac
     3061 ccatcaatcg ccagtgcggt gcgagtaaca ggtccgtatg cccatagctg gtaatggcat
     3121 tcaatgaata gagcatcgcg gtgcggccat ccggaagcgc accgagactg cgaaacgtag
     3181 ccgcccacag cgccgcttcg aagccgagca acagaatcgc cagcagcgcg ctcactccca
     3241 tgacggatac gaatatcggg aagaacttgc gccccgcgcg aatgcggcgg atgaaccgca
     3301 ccatgcgcag gttgatgagc gcgagtccga cgacgtgcag tacgagcgtc agcacgatca
     3361 gcggcagcgc ccatgcccag ttgccgtccc agctcatggc gtcagacatt gaagcctccc
     3421 cggaccttgt caccgcacgg gccgcggcgg accgcgtcgc gcgccccttg tctgaacgca
     3481 gacgatgagc agccgatgtg atggagagat ggagcgggct aacgggttcg aaccgtcgac
     3541 ctgtaccttg gcaaggtacc gctctaccaa ctgagctaag cccgcgtcct tgtgaagagc
     3601 gcgaaattct atgtacaccc cttgggaagt caaggcgagt cctcccggcg gcggtgctcg
     3661 aactctccag aaattagcgc ggtaactcac tgttaatcaa tcaattgatg tcagcgcttg
     3721 agctcgggcc aggcggctcg cagatagacg agcatcgaga cgagagtcgc cgcagcggcg
     3781 atcaccgtaa gcgcgacacc gactctatag gtgggcaacc ccagcaacgg ccaccgatag
     3841 agcatcatcg agagcccggt gatctgcagg atggttttgt acttgccgag ctgcgagacc
     3901 gccaccttgc gccgtgcccc gatctcggcc atccactcgc gcagcgcgga gatggtgatc
     3961 tcccgtccga tgataacgat cgctgtgagc acgatgagcg cacgcgggtc cttgctcacc
     4021 agaagcacga gcgccacggc cacgatcagc ttgtcggcca ccggatccag gaaagccccg
     4081 aggcgtgaga tctgtccgag gcgccgcgca aagtaaccat cgagcgaatc ggtgacgccg
     4141 gcggccgcga acagcagccc ggcagccgga tctgcccacg gatagggcat gtagaaaagc
     4201 aggatgatca gtggaatcgc cgcgatgcgc gtccaggtga gggtgttggg aaggttccac
     4261 gccatgcacg ccacctagga gcccgggtgc aggtgatcat aaagagttct tgccagcgcc
     4321 gcgccgattc cgctcacctg cgtcagatcc gcgactcccg cccgcatgat cccctgtaag
     4381 cccccgaagt gcttcagcag cgcgcggcgc ttggcgggcc caagtcccgg cacgacttcc
     4441 agcaccgact ggttgtagcg ccgtgcgcgc ttcttacgat gcccgctgat ggcgaaacga
     4501 tgcgcctcgt cgcggatgcg ctggacgaat cgtgaggccg ggccgtgcgc cacgggcatg
     4561 atgggtgact gtgctccgta cacgaacagc cgctcctgcc cggcgcgccg ctcgggcccc
     4621 ttggcgacac cgacgagtgc cagctcggca aacccgagcg cggcgagcac ttcgtgcacc
     4681 tcgcggatct ggcccaggcc gccatcgatc aacagcacat ccggcgccgg cacctctcct
     4741 tctcccacgc gccggtagcg ccgctcgagc gcctggcgaa gcgcggcgta gtcatcgcct
     4801 ggggtcacgc cgcggatatt gaagcggcga tactccttct tgaggggtcc ctcgggtccg
     4861 aacacgacgc acgcggctac cgtgccctcc ccgcccgtgt ggctgatatc gaagcattcg
     4921 aggcgctgcg gcggctcggg cagatcgagc tcccgggcga gatcggcgag catctcatcc
     4981 atgccggcgc ggtgtgcgaa gcgcatgcgc agtgcctggg cggcattctc ggcggtgagc
     5041 tcgacccaac gcgcgccgat gccgcgcgac gggcagcgaa cctccagcgc acgcccgagc
     5101 cgctcgcgca gtgcctccgc cagcgccggc gcctcctcgg tgcggctgcc cagcaggatt
     5161 tccccgggcg catccgcatt cgcgtagtac tgcatgacga atgacgtcaa cgcctcgtgc
     5221 ggctcggcga gcgcggcgcg cgggaagtaa ctgctggtgc cgaggttgcg gccaccgcgc
     5281 accagcatga cactcaccgc gtactcaccc gcttcaccgg cgatcgcgaa tacgtcggca
     5341 tcgcgcgcac cctccgccat caccacctgt tgggcctgaa tgcgcttgag agcggcgagc
     5401 tgatcgcgaa tttccgcggc tcgctcgtac tcgagccggg ccccggcctg ctccatgcgc
     5461 gcctgcagct ccgcactgac ctcctcgctg cggccttcca gtaccttcac tgctgcggcg
     5521 atgtcctgga cataggcctc acgggtaatc agcccgacgc acggtgccga gcagcggccg
     5581 atctggtgct gcagacatgg gcggctgcga ttggcaaaaa agctgtcgcg gcaattgcgg
     5641 atacggaaca gcttctgcag ctgattgagc gtgtcgcgca ccgcacccgc gctcgggaac
     5701 gggccgaaat agcgccccgg cgccgaacgg gcgccgcgat agaactccag ccgcgggtag
     5761 tcgtgctcga tatacaggtg gatgtaggga aagctcttgt catcgcgcag caccacgttg
     5821 aagcgcggct tgtgcgcctt gatcaggttg tattcgagca gcagcgcctc ggtctcggag
     5881 ttggtgaccg tgacctcgat ggcgctgatc tgtgagatga gcgcctgaac cttgggatcg
     5941 aggttgcggg gcgcaaagta gctcgccaca cgatctttca ggctgcgcgc cttcccgacg
     6001 tagagcagct cgtgcgcggc cccgtacatg cggtacacgc cgggacgccg cggcagcgat
     6061 gcgatgtatt ccttgtgatc gaacatcctt caatccgcgg ccgcgcccgt tctcgcccgg
     6121 cggccgggga gctgggcgcg ctggcaggcg cgcgccacta cctgctcgaa catttcggcc
     6181 gtgagccggc gcgtggcggt gttgtagcgg ctgcaatgat acgaatcgat cagccagcgc
     6241 gtcccatcga gctgatgctc ccggccgtgt gcgaaggggt attgcgtgcg gcgcctggcg
     6301 agcgccagca gcaggccctc gtgcgccacg cgtccgagcg ccaggtagac gcgcaccgag
     6361 cgcaactgct gcaactcggc ggcgagatag ccgttgcaac ggcgcacctc ttccggcaac
     6421 ggcttgtttg ccggtggaac gcatttcacc gtgttgatga tgcgcacctg gatgagccgc
     6481 aatccatcct cggccgagtg ggactgcggg cggctggcga gaccggcggc ataaagggtc
     6541 cggtagagca gcaccccggc gtgatcgccg gtaaagggcc gtccggtgcg attggcgcca
     6601 tgaagtcccg gggcaagccc cacgatcacg atgcgcggcc gcgccgcgcc aaaggagggc
     6661 accgggcgcg cccagtagcc tggatgccgc tggcgtatat gctcgagaaa ctcggcgagc
     6721 cgcggacagc gcgtgcagtc cgggtcgtag gcccggcctg cctcagggcg cggcggctcc
     6781 actgcgccgt tcgctacggc ggctctggct gcgctcggcg atgagcatcg caagctcgag
     6841 cgcctgctcg tgattgagtc gtggatcgac gctcgagcgg tacgcgcgcg cgaggtccgc
     6901 atcggtgagt ccgcgcgctc cgccggtgca ctcggtgaca tcgtcgccag tgagctcgat
     6961 gtgcacgcca ccgaggtgcg agccgagctc gtcatgaaga cggaaggaca ggtcgagctc
     7021 ccgcaggatg ttctcgaagc gccgggtttt caggcccccg gtggtggtct cggtgttgcc
     7081 gtgcatcgga tcgcacaccc acagcaccgt ctgaccggtt tgccgcacgg cctcgatcgc
     7141 tttcggcagt gccgccgcga cctccttcgc cccgaagcgg tgaatgaagg tgagccggcc
     7201 tggctggttc tgtgggttga gggtgtgcac gagtccctgc agccacgccg catccatcgc
     7261 cgagccgatt ttcaccccga tcgggttggc gatgccgcgg aagtactcga cgtgcgcgca
     7321 atcgagcgcc gcggtgcgga tgccgatcca gggcatgtgg gtcgagaggt tgtaccagcg
     7381 gttctggcgc ggaatgaagc gggtctgtgc ctgctcgtac agcagatgca gtccctcgtg
     7441 actcgcgtag aagtcgacca gcgtcgcttc gtgaaccgga tgcccactca tgccttcgaa
     7501 gaaatcgagc gcatcgccga tcgagcggac gatgcgttgg taggcgtcct tcagcggcgc
     7561 ctggtgcacg aagcccagat cccagtactc cgggtggtgc aggtcggcga acccaccctc
     7621 gaccagtgcg cgcacgaagt tcaacgtgag cgctgcgcgc tcgtatccgc gcagcagcag
     7681 ctcgggatcc gcagcgcgcg ccgcggcggt gaactcgggg cggttgacca gatcgccgcg
     7741 atagctcggc agcgtcgctt cgccgcgtgt ctcggtatcg gctgagcgcg gcttggcgta
     7801 ttgcccggcc atgcgtccaa cgcgaacgat gggcttcttc agtccgtgca ccagcacgag
     7861 actcatctgc aggaggatct tgagcttctt ggcgatgacg tcggattcac agtcagcaaa
     7921 agtctcggcg caatccccgc cctgcagcag gaag
//
LOCUS       DATDMP010000559         4284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1532761, whole genome shotgun sequence.
ACCESSION   DATDMP010000559 DATDMP010000000
VERSION     DATDMP010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532761"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VI195_09830"
     CDS             <1..480
                     /locus_tag="VI195_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY6824728.1"
                     /translation="KGFNKKRNSFVQVYGGDELDASLLLMPLVGFLPGSDKRIKGTVD
                     AIQKNLVQDGLVLRYNTSRVHDGLPPGEGVFLACSFWLADNLLMQGRHEEARRLFERL
                     TALCNDVGLLSEEYDPAARRFLGNFPQAFSHVALVTTAMNLSHAEKPSEQRSEKKAA"
     gene            complement(477..797)
                     /locus_tag="VI195_09835"
     CDS             complement(477..797)
                     /locus_tag="VI195_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6824729.1"
                     /translation="MTRFISLLAMGAAVSALFYLDRFNRPRSATRAAPGRSWDDYLAG
                     RVRAALPKSIQVAVHNAAVTLRGSVPYNERDRMLAAALAIPGIERVINLLEAQGRPAD
                     LGYD"
     gene            complement(839..1639)
                     /locus_tag="VI195_09840"
     CDS             complement(839..1639)
                     /locus_tag="VI195_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6824730.1"
                     /translation="MTVDERIEKFPLRISGPISYREAGRGQALLCLHGIGSDSAGWLN
                     QLEGLSDRFRVIAWDAPGYQRSAPLKDEKPSASQYAEALHGLVTRLLLKDVVIAANSL
                     GGLFAGAYARAHGDRVRHMVLISPTSGYGAADEAVRTEKREARFRMLAQIGPEGMAEQ
                     RSPTLFGKKMTPEALALARWNQRRIHPDGYRQAVWCLDSGRHLEDARHYAKRVLVVCG
                     TEDTVTPESGCKEVAAAYPRGEYRALPGLGHVAHIEDPALLNEIIAGA"
     gene            1672..2781
                     /locus_tag="VI195_09845"
     CDS             1672..2781
                     /locus_tag="VI195_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY6824731.1"
                     /translation="MRGRAEIVGGGFAGLAAACALAQRGWRVRLHERSNELRTAGAGI
                     NIYENGLRVLDAFGAREDTLRGGARHLVRETRDEHDRLLAVHRWDSRVFGVLRQRMID
                     ALAAAARRAGAEILTGSEGVSASSSGTVVFSDRTEAKADLVIAADGVNSRLRDSLGLV
                     QKRRYLPDGCIRVLIPKLAAEEATDGRTIEYWSGNRRLLYNPCSETHLYLALTMLHSD
                     TQARALPIDAALWAKAFPHLAALIRRIGDAGRYDRFEYIRLKRWSAGRVAVLGDAAHA
                     LPPNIGQGAGCAMMNALALAVHLEKANDVAAALESWERSERPLTDHAQRISLLYGMPA
                     FWPPRLRELFYSFAGRSKWLTRQRTRTARHIPTGT"
     gene            complement(2811..3212)
                     /locus_tag="VI195_09850"
     CDS             complement(2811..3212)
                     /locus_tag="VI195_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6824732.1"
                     /translation="MAKLRHIAVVVKDLEKAARFYEEVFGLKRVGEEHLEMGSGIYLS
                     DGVINLALLNYKGETGSGLKDPGNFVGAHHFGLQVDDLEAARERIEAAGGTFFFTLGK
                     SKEDSNFEMKFKDPDGVIFDISEKGWLGAKG"
     gene            complement(3212..>4284)
                     /locus_tag="VI195_09855"
     CDS             complement(3212..>4284)
                     /locus_tag="VI195_09855"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY6824733.1"
                     /translation="DLAFVQGGASETIRAPSDDRKDKEVPLVSLGSLFYEPVWLFYRQ
                     DSAQKIAKDGVLTGLPQFRGLRINTGLRGSGGPGLTNRLFAANFVDREEFKRSLLDET
                     SAVVGLLAGELDAMCLVSAPESSMVQMLLQTPGIKLFEFPNGEAYTRRYSYISPVTLP
                     RSVADLSRDVPPRDIPLIAATTSLVAREGTHPALVQLFVQAASRIHGNPGWIARAGQF
                     PSPQQSEFPLAKEAERYYRSGPPLLQRYLPFWLANLIDRMWVALVSIAVVFIPLSRLI
                     PPLYVFRVRSRIFRWYRQLRQIEERSERKDPAFPELLAELDKLDEKATRINVPLAYTD
                     ELYALRQNIDLVRERLTQGKAA"
BASE COUNT          705 a         1399 c         1423 g          757 t
ORIGIN      
        1 aaggggttca acaaaaagag aaacagcttt gtgcaggtct acggcggaga tgagctcgat
       61 gcgtcactgc tgctgatgcc gctagttgga ttcttgccgg gcagcgacaa acgcatcaag
      121 ggcaccgtcg atgcgatcca gaagaatctg gtgcaagatg gattggtgct gcgctacaac
      181 acgtctcgcg tgcacgacgg cttgccgcct ggcgaaggcg tttttctcgc gtgcagcttc
      241 tggctggccg acaatctcct catgcagggc cgtcacgagg aggcgcggcg actcttcgag
      301 cggctgaccg ccctttgcaa cgacgtcggc ctcctctcgg aggaatacga tcccgccgcg
      361 cggcgcttcc tgggtaattt tccgcaggcc ttttcgcatg tggcgctcgt gaccaccgcg
      421 atgaacctca gccacgccga gaagccctcg gagcagcgct ccgagaagaa agccgcctag
      481 tcgtagccca gatcggcggg gcgcccttgc gcttccagca gattgatgac gcgctcgatg
      541 ccgggaatgg cgagcgctgc cgcgagcatg cggtcgcgct cgttatacgg caccgagcct
      601 ctcagcgtga ctgcagcgtt gtgcacggcc acctggatgg atttcggcag tgctgcgcgg
      661 acccggcccg ccaggtagtc atcccacgac cggccgggtg ccgccctcgt cgcgctgcgc
      721 ggacgattga agcgatcgag atagaacagg gcacttaccg cagcgcccat tgccaacaaa
      781 gaaatgaatc gggtcatggg gttgtcctgc taacggtcca cgcccgctct ccagcaagct
      841 acgctcccgc gatgatttcg ttaaggagcg cgggatcctc gatgtgcgca acatggccta
      901 gtccaggcag cgcacggtat tcccctctgg ggtacgcggc ggccacttcc ttgcagccgc
      961 tttccggggt gaccgtgtcc tccgttccgc aaaccacaag gacccgcttt gcgtagtggc
     1021 gcgcgtcttc gaggtggcgg ccggagtcca ggcaccagac ggcctggcgg tagccgtcgg
     1081 gatggatcct gcgctggttc catcgcgcaa gcgccagggc ctcgggtgtc atcttctttc
     1141 cgaacagcgt gggcgagcgc tgctcggcca tgccttccgg gccgatttgc gccagcatcc
     1201 tgaatcgcgc ctcgcgcttt tccgtgcgca ccgcctcgtc cgccgcgccg tagccgctcg
     1261 tggggctgat gaggaccatg tgcctgaccc ggtcgccgtg cgcgcgcgca taggcgcctg
     1321 caaagagccc cccgagcgaa ttcgcggcga tgaccacgtc tttcaggagc agccgggtga
     1381 cgaggccgtg aagggcttcg gcgtactggc tcgcgctcgg tttctcgtcc ttgagcggcg
     1441 ccgagcgctg gtagcccggc gcatcccagg cgatgacgcg aaagcgatcg ctcaagcctt
     1501 cgagctggtt cagccatccc gcggagtccg agcctatgcc gtgcaggcag agcagggcct
     1561 ggccgcgccc ggcttcgcga tagctgatcg ggccggaaat gcgaagcggg aatttctcga
     1621 tgcgctcatc gacggtcatg cgggctcctc ggatcgctta ttctcccgca catgcggggc
     1681 agggcagaga tcgtaggagg cggtttcgca ggattggccg cggcttgcgc gcttgcgcag
     1741 cgaggctggc gcgtgcggct gcacgagcgc tcgaacgagc tgcgcaccgc cggggcgggc
     1801 atcaacatct atgaaaacgg cctgcgggtc ctcgatgcat tcggcgcgcg cgaggacacg
     1861 ctgcgcggcg gcgcccgcca cctcgtgcgc gaaacccgcg acgagcacga ccggcttctc
     1921 gcggtgcacc gctgggacag ccgcgtgttc ggcgtcctgc gccagcgcat gatcgatgcg
     1981 ctcgcggccg cggctcgccg cgcgggtgcc gagatcctga ccgggtccga aggcgtttct
     2041 gcctcttcga gcgggacggt cgttttttcc gatcggaccg aagccaaggc ggaccttgtc
     2101 atcgcggcgg acggcgtgaa ctcccggctt cgcgattcgc tcgggctggt gcagaagcgg
     2161 cgctacctgc ccgacggctg cattcgcgtc ctcatcccca agctcgcagc ggaagaagcc
     2221 accgacgggc gcacgatcga gtattggtcc ggcaaccgca ggctcctcta caacccgtgc
     2281 agcgagacgc acctctacct cgcgctcacg atgcttcatt cggacacgca ggcgcgcgcg
     2341 ctgccgatcg acgccgcgct atgggcgaag gcgtttcccc acctcgcggc cctcatccgg
     2401 cgcatcggcg atgccggccg ctacgatcgc ttcgaataca tccggctcaa gcgctggtcc
     2461 gccggccgcg tagcggtcct cggcgatgcc gcgcatgcgc ttccgccgaa catcgggcag
     2521 ggcgcggggt gcgccatgat gaatgcgctc gctctcgccg tccacctgga gaaggcgaac
     2581 gacgttgccg ccgcgctcga gtcgtgggag cgctccgagc gtccgctcac tgatcacgcg
     2641 cagaggatct cgctcctcta cggcatgccg gctttctggc cgcccaggct gcgcgagctt
     2701 ttttattcgt ttgccgggcg ctcgaagtgg ctcacccggc agcgcacgcg cacggcgcgg
     2761 cacataccta cggggacatg aggtcgagag aagtcggtgc ctggccccga ttagcccttg
     2821 gcgccgagcc agcccttttc ggagatgtcg aagatcacgc cgtcgggatc cttgaatttc
     2881 atctcgaagt tcgagtcttc cttcgatttt ccgagggtga agaagaacgt cccgccggcg
     2941 gcctcgatgc gctcgcgcgc ggcctcgagg tcgtccacct gaaggccgaa gtggtgggcg
     3001 ccgacgaagt tcccagggtc cttgaggccg gatccggttt cgcccttgta gttgagcagc
     3061 gcaaggttga tgacgccgtc ggagaggtag atgcccgagc ccatctcgag atgctcctcg
     3121 ccgacgcgct tcaggccgaa cacttcctcg tagaaccgcg cggccttctc gagatccttc
     3181 actacgaccg cgatgtgcct gagcttcgcc attaggccgc ctttccttgc gtcaagcgct
     3241 ccctcaccag gtcgatattc tgccgcagcg cgtagagctc gtccgtatac gcgagcggga
     3301 cgttgatgcg cgtggctttc tcgtcgagct tgtcgagctc cgccaggagc tcggggaacg
     3361 cggggtcctt gcgctcgctg cgctcctcga tctgccgcag ctgccggtac cagcggaaga
     3421 tcctcgagcg cacgcggaaa acatacagcg gcgggatcag gcgcgatagg ggtatgaaga
     3481 ccactgcaat ggatacgagc gccacccaca tgcggtcgat caggttcgcg agccagaagg
     3541 gcaggtagcg ctgcaggagc ggcgggccgc tgcggtaata gcgctcggcc tccttcgcca
     3601 gcggaaattc gctctgctga ggggatggaa actggccggc gcgcgcgatc catccgggat
     3661 tgccgtggat gcggctcgcg gcctgcacga agagctgtac cagcgcggga tgagtgccct
     3721 ccctcgcaac aagagatgtc gtcgcggcga tgagcggaat gtcgcgaggc ggcacgtcgc
     3781 gcgaaagatc ggcgacgctc cgaggcaggg tcaccgggct gatatacgaa tagcgccgcg
     3841 tgtaggcctc gccgttgggg aactcgaaga gcttgatgcc cggcgtctgg agcagcatct
     3901 gcaccatgga ggactcgggc gcggacacca ggcacattgc gtcgagctcg cccgcaagga
     3961 gcccgaccac ggccgaagtc tcgtcgagga ggctgcgctt gaactcctcg cggtcgacga
     4021 agtttgcggc aaagaggcgg ttggtgagcc cgggtccgcc gctgccgcgc aagcccgtat
     4081 tgatgcgcag gccgcggaat tgcggcagcc cggtcagcac gccatccttg gcgattttct
     4141 gtgcgctgtc ctggcggtag aagagccaga ccggctcgta gaaaaggctg ccgagcgaga
     4201 ccagaggcac ctccttgtcc tttcggtcat ccgacggcgc gcggatcgtc tcgctcgcgc
     4261 cgccctgcac gaaggcgagg tcga
//
LOCUS       DATDMP010000560         3115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1199552, whole genome shotgun sequence.
ACCESSION   DATDMP010000560 DATDMP010000000
VERSION     DATDMP010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199552"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VI195_09860"
     CDS             complement(<1..516)
                     /locus_tag="VI195_09860"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY6824734.1"
                     /translation="MPGKLILVRHGQSIWNLENLFTGWTDVDLSAQGRAEAIAAGHEL
                     LKEQLRIDFAFTSVLKRAIRTLWIMLDEMDRMWLPVERSWRLNERHYGTLQGLDKAQT
                     AALHGEAQVKIWRRSYDIAPPPLGADDRRHPRFDARYAAVDPQELPASESLKDTLARV
                     LPFWHARIAPEL"
     gene            complement(548..>3115)
                     /locus_tag="VI195_09865"
     CDS             complement(548..>3115)
                     /locus_tag="VI195_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY6824735.1"
                     /translation="IRATLRTNYYQPGSDGAPKSYLSFKFDPAQIADLPLPKPKFEIF
                     VYSPRVEGVHLRMGYVARGGIRWSDRREDFRTEVLGLMKAQNVKNTVIVPVGAKGGFV
                     PKRLGGSREEIQAEVVACYQTFIRGLLDLTDNIVAGRIVPPPQLVRRDTDDPYLVVAA
                     DKGTASFSDIANAISADYGFWLGDAFASGGSAGYDHKKMGITARGAWECVKRHFREMG
                     IDTQKSDFTVIGIGDMSGDVFGNGMLLSRHIRLVAAFDHRHIFIDPEPDPAVSFGERA
                     RLFALPRSSWDDYDRSKLSRGGGVFARSAKSIVLSPEARGLLLLEGANAAPNDVIRAI
                     LTLPVDLLWNGGIGTYVKASDERNAEVGDRANDVLRINGVQLLAKVVGEGGNLGLTQR
                     GRIEYALARGRLNTDFIDNSAGVNTSDVEVNIKILLNPLVADGKLARGERNRLLARMT
                     SEVAALVLRNNYLQSQAISTLELQGASRLPEYQHLIRGLEREGVLNRTLEFLPSDDEL
                     SDRRKSGVGLTRPELAILLAYSKIWLNNHLLASDVPEDPYLSAELVRYFPTPIQQRFP
                     RAIRRHRLRREIIATATTNSLVNRMGPTFVPRVQGDTGAEPAQIARAYTAAREIFAMR
                     AVWEQIEALDNKVRAQLQYEAAFQTSRLLRHATYWLLTTRPGGLQVDAAVAEFRNAVQ
                     QLETATPGVLTGAELARFEEARKSYGESGLPAALAARIASLEALNAALDVAEIAAGHR
                     VSVPEAARVYFEVGARVGCDWLQARIEKLLVEGPWQAVARTGLRDAALRVHRRLAERV
                     LDHKSRGTAPARVTDWIESGGQELAHWQRTVAEMRAAEAADFATLTVGVEALRKLAN"
BASE COUNT          487 a         1029 c         1070 g          529 t
ORIGIN      
        1 cagctcgggc gcaatgcgcg cgtgccagaa gggcagtacg cgggcgaggg tgtccttcag
       61 cgactcgctc gcgggcagct cctgcgggtc gaccgcggca taacgcgcat cgaaccgcgg
      121 gtggcgccga tcgtccgcgc cgagcggcgg cggtgcgatg tcgtagctgc gccgccagat
      181 cttcacctgc gcctcgccgt gcagcgccgc cgtctgcgcc ttgtcgagtc cctgcagcgt
      241 accgtagtgc cgctcgttga gccgccagga gcgctcgacc ggtagccaca tgcggtccat
      301 ctcatcgagc atgatccaca gtgtgcggat ggcgcgcttc agcaccgagg tgaaggcgaa
      361 gtcgatgcgg agctgctcct tcagcagctc gtgaccggcc gctatcgcct cggcgcgtcc
      421 ctgagccgaa aggtcgacat cggtccagcc ggtgaacaga ttctccagat tccagatgct
      481 ctggccgtga cgaacgagga tcagcttgcc tggcacgatc aggtcctgcg ttgtgaatcg
      541 cggcgcatca gttggcgagt ttgcgcaacg cttcgacacc caccgtgagg gtcgcgaagt
      601 cggcggcctc cgcggcgcgc atctctgcga ccgtgcgttg ccagtgagcc agctcctgac
      661 cacccgactc gatccagtcg gtgacgcggg ccggagcggt gccacggctc ttgtgatcga
      721 gcacccgctc ggcgaggcgc cggtgcacgc gcaacgctgc atcgcgcagc ccggtacgcg
      781 cgactgcctg ccacgggccc tcgaccagga gcttctcgat gcgcgcctgc agccagtcgc
      841 agccgacacg cgcacccacc tcgaaataga cccgtgccgc ctccggcacg ctgacgcggt
      901 gaccggcggc gatctcggcg acatcgagcg cagcgttgag cgcttcgagg ctcgcaatgc
      961 gcgcggcgag tgccgccggc aggccagact cgccgtagct cttgcgcgct tcctcgaagc
     1021 gcgccagctc cgcgccggtg agcaccccgg gggtcgcggt ctcgagctgc tgcacggcgt
     1081 tgcggaactc ggccacggcg gcatccacct gcagcccgcc gggacgcgtg gtgagcagcc
     1141 aataggtggc gtgacgcagc agtcggctgg tctggaaggc ggcctcgtat tgcagctgcg
     1201 cccgcacctt gttgtcgagc gcttcgatct gctcccatac cgcacgcatc gcaaagatct
     1261 cgcgcgcggc ggtgtaggcg cgcgcgatct gagccggctc tgcgccggtg tcgccctgca
     1321 cccgcggcac gaacgttggc cccatgcggt tgacgaggct gttggtggtg gcggtggcga
     1381 tgatctcacg gcgcagtcga tgacggcgga tcgcgcgcgg aaagcgctgc tgtatcgggg
     1441 tgggaaagta acgcaccagc tccgccgaga gataaggatc ctcgggcacg tccgaggcga
     1501 gcaggtggtt gttgagccag atcttgctgt aggcgagcag gatggccagc tccgggcgcg
     1561 tgagcccgac gccgctcttg cggcgatcgg agagctcatc gtcgctcggc aggaattcga
     1621 gcgtgcggtt caacacgcct tcgcgctcga gcccgcggat gagatgctgg tattccggca
     1681 gccgcgaggc gccctgcaac tcgagcgtgc tgatcgcctg gctctgcagg tagttattgc
     1741 gcaataccag cgccgccacc tcggaggtca ttcgcgccag caggcggttg cgctcgccgc
     1801 gcgccagctt gccatcggcc accagcgggt tgagcagaat cttgatgttg acttcgacgt
     1861 cggaggtatt gacgcccgcg gagttgtcga tgaagtcggt attgaggcgc ccgcgcgcga
     1921 gcgcatattc gatgcggccg cgttgcgtca ggccgagatt gccgccttcg cccaccacct
     1981 tggcgagtag ctgcacgccg ttgatgcgca gcacgtcgtt ggcgcggtcg ccgacctcgg
     2041 cgttgcgctc gtcgctcgcc ttgacgtagg taccgatgcc gccgttccac agtagatcga
     2101 ccggcagcgt caggatggcg cggatgacgt cgttgggggc ggcattcgcg ccctccagca
     2161 gcagcaggcc gcgcgcctcg ggcgagagca cgatcgattt ggcggagcgc gcgaacactc
     2221 cgccgccgcg cgagagctta ctgcgatcgt agtcgtccca actcgatcgc ggcagcgcaa
     2281 aaagccgcgc ccgttcgccg aagctcaccg ccgggtccgg ctcggggtcg atgaagatgt
     2341 ggcggtgatc gaaagcggcc acaaggcgga tgtggcgcga cagcagcatg ccgttgccga
     2401 acacgtcgcc cgacatgtca ccgatgccga tgacggtgaa gtcgctcttc tgcgtatcga
     2461 tgcccatctc gcggaagtga cgtttgacgc actcccacgc accgcgcgca gtaatgccca
     2521 tcttcttgtg gtcgtagccg gccgagccgc cggaggcgaa cgcgtcgccg agccagaagc
     2581 cgtagtcggc ggagatggca ttggcgatat cggagaagct tgccgtgccc ttgtccgcag
     2641 cgaccaccag gtatgggtca tcggtatcac ggcgcaccag ctgcggcggc ggcacgatgc
     2701 gtccggcgac gatgttgtcg gtgaggtcga gcaggccgcg gatgaaggtc tgatagcagg
     2761 ctacgacctc cgcctggatt tcctcgcggc tgccgccgag gcgcttcggc acgaagccgc
     2821 ccttggcgcc caccggcacg atgacggtgt tcttgacgtt ctgcgccttc atcaggccga
     2881 gaacttcggt gcggaagtcc tcgcgccggt cggaccagcg gattccgccg cgcgccacgt
     2941 agcccatgcg caggtgcaca ccctcgacgc gcgggctgta gacgaagatc tcgaacttcg
     3001 gcttcggcag cggcaggtcg gcgatctgcg ccgggtcgaa cttgaacgac aggtagctct
     3061 tgggtgcgcc gtcactgccc ggctggtagt agttggttcg cagcgtcgcc cgtat
//
LOCUS       DATDMP010000561         3171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_90149, whole genome shotgun sequence.
ACCESSION   DATDMP010000561 DATDMP010000000
VERSION     DATDMP010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90149"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VI195_09870"
     CDS             <1..622
                     /locus_tag="VI195_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY6824736.1"
                     /translation="RIRARQGKQAAVSAGERAAVESHIHSRPQHPRPRSDWDLLARFR
                     ERAIGLASTVDMADSLGSVPAAAARYLKSNSLPLTAACWAEFSTLDWRSAGIEIAARP
                     ALDADQVGITGAFCAIAETGTLVTVTGTQTPAVTSLLPETHIAVVAVSRIVRGMEEAW
                     QLARDELGQLPRAVNFISGPSRTADIEQTVTLGAHGPYRVHVILVA"
     gene            793..2001
                     /locus_tag="VI195_09875"
     CDS             793..2001
                     /locus_tag="VI195_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6824737.1"
                     /translation="MTQLSFLPSFPFSTDPLLLFGLLLLAGLAGGELARRVLRLPRIV
                     GYALIGLALGTSGVGALDAGLLKDAWIFVDIALGLILFELGRRLHFAWLRNDRWLLAT
                     GIVESALSFGCVYVALSLFDVRPIHAALAAAIGIATSPAVVLLVAQELRAEGQVTERA
                     MNLVALNSVIAFVLTTMLLSWLHHESRASWAMIALHPLYLLGGSLLLGYFASLAAILL
                     AQWVGKHSERQLVMLLSLILLTIGAARMLELSVLLALLAFGVLSRNLDGRHDLMAVDL
                     NGVGQVFYVVLFVVSGAQLQVLGLMTGGVLALVYVVARFIGKSIGVMSLTYFSGARGG
                     SAGLLCIALTPMSGIALAMVHETASLYPRFGAQLGAIVLSAVLILELVGPIAVQFALR
                     RAGEAREAAP"
     gene            1998..3131
                     /locus_tag="VI195_09880"
     CDS             1998..3131
                     /locus_tag="VI195_09880"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEY6824738.1"
                     /translation="MSEMNFRPSPGLSVGVELELQILNSRDFDLARDAADLIALLEKA
                     KIPGAVKPEITESMVELNSSIHKGHDALAAELRTLRDAVTQAAQRLNVRIAGGGSHPF
                     HMWADRRIYPTERFNHLLSLYGYLAKQFTIFGQHVHIGCPDGDAAIYLSHILSHYVPH
                     FIALSASSPFQQGEDTAYQSSRLNTVSAFPLSGQIPFVGSWDEFVKYFNKMRGFGIVE
                     SMKDFYWDIRPKPEYGTVEIRVFDTPLTVERAALVAAYAQTLARHILTERPLAPSRDV
                     YVLYNYNRFQACRYGLAAKIVDAYTQRHVDLKEEVLETLTLIAQHAAELGNAQALARL
                     HDEVVAGRTDSDWLRAANKEKGSLHDVARLQSDLWMGLGQKPP"
BASE COUNT          503 a         1008 c         1038 g          622 t
ORIGIN      
        1 gcgcatccgc gcgcggcagg gcaagcaggc ggccgtttcc gccggggagc gcgcggcggt
       61 cgaatcgcat atccattccc gtccgcagca cccgcggcca cgttccgatt gggatctgct
      121 cgcccgcttc cgggaacgtg ccattgggct cgcgagtact gtggatatgg ctgattccct
      181 tgggtcggtt ccagccgcgg ccgcgcgcta cctcaagtcc aattcgcttc cgttgacggc
      241 tgcgtgctgg gcggagtttt caacgctgga ctggcggtcg gccggcatcg agatcgccgc
      301 gcgcccggcg ctcgatgccg accaggtcgg cataaccgga gcgttctgcg caatcgcgga
      361 gaccggaacg ctggtgacgg tgacggggac gcagacacct gccgtcacga gcctgctgcc
      421 ggaaacccac attgccgtgg tggcggtgtc gcgaatcgtg cgcggcatgg aggaggcatg
      481 gcagctcgct cgggatgagc tgggacagct gccgcgcgcg gtcaacttca tttcgggccc
      541 ctcgcgcacc gccgacatcg agcagaccgt cacgctcggc gcgcatgggc cataccgcgt
      601 gcacgtcatt ttggttgcat gacaaatcaa taccttatgc gccgcggaac ttcctgccga
      661 tccgcggtcc aatagcacga taacaatcca tccgcagttt tccgcgttcg cgaggagtac
      721 gcggacgatc cagcatccag ttgttgacgt agccggggcg ccctggcgcc gcggcacgag
      781 cgggggcccg ccatgacaca actgagcttt ctcccgtcgt tcccgttttc caccgatccg
      841 ttgctgctgt tcggcctgct gcttctcgcg gggctcgccg gcggtgagct ggcgcggcgc
      901 gtgctgcgtc tgccacgcat cgtcggctac gccctgatcg ggcttgcgct cggtacgagc
      961 ggggtcggtg cgctcgatgc cggactgtta aaggacgcct ggattttcgt cgacatcgcg
     1021 ctggggctga tcctgttcga gctcggcagg cggctccatt tcgcctggct gcgcaatgac
     1081 cgctggttgc tcgcgacggg gatagtcgag agcgcgctgt cattcggctg cgtctacgtt
     1141 gcgctgagcc tctttgacgt caggccgatt cacgcggcac tggcggcggc gatcggcatc
     1201 gcaacctcgc ccgcggtggt gctgctggtc gcgcaggagc tcagggccga gggccaggtc
     1261 accgagcgcg ccatgaacct ggtggcactc aacagcgtga ttgcgttcgt gctgacgacg
     1321 atgctgctgt cctggctgca ccatgagtcc cgtgcaagtt gggccatgat cgcgctgcac
     1381 cctctctact tgctcggcgg gtcgctgctg ctcggctatt tcgccagcct tgccgcgatc
     1441 ctgctcgcac aatgggtcgg caagcattcc gaacgccaac tcgtgatgct gctgtcgctg
     1501 atcttgctga ccattggcgc ggcgcgcatg ctggagctct cggtgctgct ggcgctgctc
     1561 gcgttcggcg tgctctcgcg caaccttgac gggcgccacg acctgatggc agtggacctg
     1621 aacggcgtcg gccaggtctt ctacgtcgtg ctgttcgtgg taagcggcgc gcagctgcag
     1681 gtgctgggac tgatgaccgg cggggtgctc gcgctcgttt acgtggtggc gcgcttcatc
     1741 ggcaagtcga tcggcgtcat gagcctcacc tacttcagcg gcgcgcgcgg cgggtcggcc
     1801 gggttgctgt gcatcgcatt gactccgatg tcgggaattg cgctggcgat ggtacacgag
     1861 accgcgagcc tctacccgcg gttcggcgcg cagcttggcg caatcgtgct gtcggcggtc
     1921 ctgatcctgg agctggtggg gccgatcgcg gtgcagttcg cactgaggcg cgccggcgag
     1981 gcgagggagg ccgcaccatg agcgagatga atttcaggcc gtcgccgggg ctttcggtcg
     2041 gcgtcgagct cgagctgcag atactcaatt cgcgcgattt cgaccttgcg cgcgacgcgg
     2101 ccgacctgat cgcgttgctg gaaaaagcaa agatcccggg cgccgtaaag ccggaaatca
     2161 cggagagcat ggtcgagctg aacagctcga tccacaaggg ccatgacgca cttgcggcgg
     2221 aactgcggac cctgcgcgac gccgtcaccc aggcggcgca gcgcctgaac gtgcggatcg
     2281 cgggcggagg ctcgcacccg tttcacatgt gggcggaccg gcgtatctat cctaccgagc
     2341 gcttcaacca ccttttgtcg ctctacggct acctggcgaa gcagttcacg atcttcggcc
     2401 agcacgtgca catcggttgc ccggacggcg acgcggcaat ttacctgagc cacatactct
     2461 cgcattacgt gccgcatttc atcgcgttgt cggcatcttc tcccttccag cagggcgagg
     2521 acaccgccta tcaatcctcg cggttgaata ccgtcagcgc atttccgctc tcggggcaga
     2581 ttcccttcgt cggctcatgg gatgaatttg tcaaatattt caataagatg cgtggcttcg
     2641 gcattgtcga gagcatgaag gatttctact gggacatccg ccccaagccg gagtacggga
     2701 cggtcgagat ccgcgttttc gacacgccgc tgacggtcga acgtgcggcg ctcgtggccg
     2761 cctacgcgca gacactggca cgccacatcc tgaccgaacg gccgctcgcg ccgtcgcgcg
     2821 atgtctacgt gctctacaac tacaaccgct tccaggcctg ccgctacggt ctcgcggcga
     2881 agatcgtcga cgcctataca cagcgccacg tcgacctgaa ggaggaggtg ctcgagacgc
     2941 tgacgttgat tgcgcaacac gcggcggaac tcggcaatgc acaggcgttg gcgcgtttgc
     3001 atgacgaggt cgttgccggg cgcacggatt cggattggtt gcgcgccgcc aacaaggaga
     3061 aggggtcctt gcacgatgtt gcacgcctgc aaagcgatct ttggatgggg ctcggccaga
     3121 aacccccgtg aacttgcgac acccgcggca atctaattca tgttgcctca a
//
LOCUS       DATDMP010000562         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_756708, whole genome shotgun sequence.
ACCESSION   DATDMP010000562 DATDMP010000000
VERSION     DATDMP010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756708"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="VI195_09885"
     CDS             complement(<1..1228)
                     /locus_tag="VI195_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY6824739.1"
                     /translation="MAAVNQAINQAIGAELIPDLADDMKSHSAAAAAAKLEHYGGAAI
                     ASALSRLAPGFAQDVLTALPAEARERAFATASADLTSQWHRNGAYPANTIGRMMEPLV
                     AAYAPEQTVGETIDKLRELVKTTFITYVYVLDPQERLLGLVTMRDLLFSENEATLRNV
                     MFRDPFALQAAMPLMDAMKLVLDRHYPVYPVVDADKRLVGLLRGQSMFEAQAIEISLQ
                     AGSMVALEKEERVATHWWRSLKLRHPWLQFNLLTAFLAAAVVGLFQDTLDRLVILALF
                     LPVLAGQSGNTGCQALAVTLRGMTLGELKRGAARPLVAKETWVGCLNGAGVGLMAALG
                     MYITAVGQHQERPFMLAFVVFAAMVLSCIASSLSGALVPLTLKRFGFDPATASSIFLT
                     TATDCVSMGTLLLLATI"
     gene            1321..1866
                     /locus_tag="VI195_09890"
     CDS             1321..1866
                     /locus_tag="VI195_09890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY6824740.1"
                     /translation="MTPRAITIRLAEPRDAQAIALMSRDFIEAGLGWKYDASRVIRAI
                     RDRETLAVVACDAKSGAATRAAVNGFAIMEFGDERAHLVLLAVRPSHRRTGIGQRMLE
                     WLVESARCAGIASIHLELRAGNEAARRFYRAMGFYETVLVPGYYRSGEGRKEGALRML
                     RVLRVPGPLPAAWRPPRLEDR"
     gene            complement(1874..>2249)
                     /locus_tag="VI195_09895"
     CDS             complement(1874..>2249)
                     /locus_tag="VI195_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEY6824741.1"
                     /translation="LNTLFVRPEWRWNVGRGGAFEFTPKFYAYLSNGENPDIASYRGY
                     VDWRVRHDAGGQWITTAVDRVGTAGKGSLLVDMSRRTRDFKVGPVSGYLHLQYFNGYG
                     ESLLDYNVRRPWQLRIGVAIVP"
BASE COUNT          385 a          768 c          733 g          363 t
ORIGIN      
        1 gaatcgtcgc cagcagaagc agcgtcccca tgctcacgca gtcggtggca gtcgtgagga
       61 agatgctgga cgcggtcgcc gggtcgaagc cgaagcgctt cagcgtgagc ggcacgagcg
      121 cgccggagag actgctcgcg atgcacgaca gcaccatggc tgcgaaaacc acgaacgcga
      181 gcatgaaggg acgctcctga tgctggccga ccgcggtgat atacatgccc agcgccgcca
      241 tgaggccgac gccggcgccg ttcaggcagc cgacccaggt ttccttggcg acgagcggcc
      301 gcgccgcgcc gcgcttgagt tcgccgagcg tcatgccgcg cagcgtcacg gcgagcgcct
      361 gacagccggt attgcccgac tggcccgcca gcaccggcag gaatagcgcg agtatcacga
      421 gacgatcaag cgtgtcctgg aagaggccca ccaccgccgc cgcgagaaac gccgtcagca
      481 ggttgaattg cagccacggg tgacgcagct tgaggctgcg ccaccagtgc gtggcgacac
      541 gctcttcctt ctccagggcc accatgctgc ccgcctgaag tgagatctcg atcgcctggg
      601 cttcgaacat gctttgcccg cgcagcagcc cgaccagacg cttgtccgca tcgaccaccg
      661 gatacacggg atagtggcga tcgagcacca gcttcatcgc atccatgagc ggcatcgccg
      721 cctgcagcgc gaacggatcg cggaacatca cgttacgcag cgtcgcctcg ttctcgctga
      781 agagcaggtc acgcatggtg accaggccga gcaggcgctc ctgcggatcg agcacataga
      841 cgtaagtgat gaacgtcgtc ttcaccagct cgcgcagctt gtcgatcgtc tcgccgaccg
      901 tctgctcggg cgcataggca gcgaccagcg gctccatcat gcggccgatg gtgttggccg
      961 gatacgcgcc attcctgtgc cactgactag tgagatcggc gctcgcggtg gcgaaggcgc
     1021 gttcgcgcgc ctcggccggc agtgcggtga gcacgtcctg cgcgaagccc ggcgccagac
     1081 ggctgagcgc gctggcgatc gctgcgccgc cgtagtgctc cagcttcgcc gccgctgcgg
     1141 ccgccgaatg gctcttcata tcgtcggcga gatcgggaat cagctctgcg ccgatcgcct
     1201 gattgatcgc ctggttgact gcggccatgg ctgtcgcctc cctttccccg cttcttttcc
     1261 cgctggtccg cgcctcccgc gcagcgccaa gtcgaggacg gatcatggca caatgtgttc
     1321 atgaccccac gggccatcac catccggctc gccgagccgc gcgacgcgca agcgattgcg
     1381 ctgatgtcgc gtgactttat cgaggccgga ctcggctgga agtacgacgc gtcacgagtg
     1441 attcgtgcga tccgtgatcg cgagacgctc gcggtggtgg catgcgacgc aaaatccggc
     1501 gctgccacgc gcgccgcggt caacggcttc gccatcatgg agttcggcga cgagcgcgcg
     1561 catctcgtac tgctcgccgt gcggccgtcg caccgccgca ccggcatcgg ccagcgcatg
     1621 ctcgagtggc tggtggagtc ggcacgctgc gccggcatcg ccagcatcca cctggaactt
     1681 cgcgccggca acgaagcggc ccggcgcttc taccgcgcca tggggttcta cgagacagtc
     1741 ctcgtaccgg gctattaccg cagcggcgaa ggacgcaagg aaggcgcgct gcgcatgctg
     1801 cgcgtgttgc gcgtgccggg accgctgccg gccgcgtggc ggccgccgcg cctcgaagac
     1861 cgctgacctc gcctcacggc acgatcgcca cgccgatgcg cagctgccac ggccggcgta
     1921 cgttgtagtc gagaaggctc tcgccgtaac cattgaagta ctgcaggtgc aggtagccgc
     1981 tcaccggccc caccttgaag tcgcgcgtgc ggcgcgacat gtcgacaagc aagctgcctt
     2041 ttccggcggt accgacgcgg tcaaccgccg tagtgatcca ctggccgccg gcatcgtgcc
     2101 gcacccgcca gtcgacgtag ccgcgatagc tggcgatgtc agggttctcg ccattcgaca
     2161 gataggcgta gaacttggga gtgaactcaa aggcgccgcc gcgaccgacg ttccagcgcc
     2221 attccgggcg cacgaatagt gtgttaagc
//
LOCUS       DATDMP010000563         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1423732, whole genome shotgun sequence.
ACCESSION   DATDMP010000563 DATDMP010000000
VERSION     DATDMP010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423732"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /gene="purH"
                     /locus_tag="VI195_09900"
     CDS             complement(<1..259)
                     /gene="purH"
                     /locus_tag="VI195_09900"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY6824742.1"
                     /translation="MSKIQRALLSVSDKSGIADLARALHALGIELISTGGTAKLLEKE
                     GLPVLEVAAYTWFPEMLDGRVKTLHPKSHGGFLARRDDPAHV"
     gene            complement(259..498)
                     /locus_tag="VI195_09905"
     CDS             complement(259..498)
                     /locus_tag="VI195_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6824743.1"
                     /translation="MSRGNELADCVKRLLERYFKDLDGEKPTSVYEMVLKNIEKPMIE
                     TVLGHAQGNQSLAAEMLGINRNTLRKKMQQLRIKG"
     gene            complement(495..1502)
                     /gene="dusB"
                     /locus_tag="VI195_09910"
     CDS             complement(495..1502)
                     /gene="dusB"
                     /locus_tag="VI195_09910"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY6824744.1"
                     /translation="MKIGAHVLRNAVIVAPMAGITDRPFRTLARKFGAAMAVSEMVSA
                     RPELRASRKTRLRLDHRGETGPISVQIAGADPLMLADAARQNVDQGAQIIDINMGCPA
                     KKVCNVSAGSALLADEALVGRILDAVVAAVPVPVTLKIRTGPSPERRNALRIAAIAEA
                     AGVQMLAVHGRTRACAFAGQAEYRTIAEVKSRARIPVVANGDVRTPAQARAVLDLTGA
                     DGIMIGRAAQGRPWLFRDIAHYLETGLAAPAVQIDEIAAVLHEHLEGLYSLYGEEQGV
                     RIARKHIGWTVCSLPGGEALRRSTNLVVDARSQVLAVREFFVRLAANDAGLAEPERLA
                     A"
     gene            complement(1551..>2148)
                     /locus_tag="VI195_09915"
     CDS             complement(1551..>2148)
                     /locus_tag="VI195_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6824745.1"
                     /translation="FESGEAETAHERFTSEGPLAVLPLQRGSAVIWTLPPARAQDLAL
                     GSAEVFLRALCAAAPHGAGRPIAVEERSAQPLALRVRRTRVAERQVYIGNAAQTLHPV
                     AGQGLNVGLRDAWELAQILRNAGDPGDSRLLARYASRRRIDAQAAIRLTDGLARAFLG
                     SGRLAASLRGAALTALDILPAPRRFFARRMIYGASALP"
BASE COUNT          339 a          721 c          743 g          345 t
ORIGIN      
        1 cgacgtgcgc gggatcgtcg cggcgggcga gaaacccgcc gtggctcttg gggtgcaggg
       61 tcttcacgcg gccatccagc atttcgggaa accacgtata ggccgcgacc tcgagaacgg
      121 gcagtccttc cttctccagg agctttgcgg tcccgcccgt ggagatcagc tcgatgccga
      181 gcgcgtgcag cgcgcgagcg aggtccgcga ttcccgactt gtcggaaacg ctcagcaggg
      241 ccctttggat tttcgacact accctttgat gcgcagctgc tgcatcttct tgcgcaaggt
      301 gttgcggttg atcccgagca tctcggcggc gagcgattgg ttgccctggg cgtggccgag
      361 gaccgtctcg atcatgggct tctcgatgtt cttgagcacc atctcataca ccgacgtggg
      421 cttctctccg tcgaggtcct tgaagtagcg ctcgagcagg cgcttcacgc agtcggcgag
      481 ctcgttgccg cgactcatgc cgccagcctt tcgggctcgg cgagtcccgc gtcgttggcc
      541 gcgagccgca cgaagaactc gcgcaccgcg agcacctggg agcgcgcgtc caccacgaga
      601 ttggtcgagc ggcgcagcgc ttccccgccg ggcagcgagc agaccgtcca gccgatgtgc
      661 ttgcgggcga tgcgaacgcc ttgctcttcc ccatagaggc tgtacaggcc ctccaggtgt
      721 tcgtgcagga ccgcggcgat ttcatcgatc tgcactgccg gcgcggccaa gcccgtctcg
      781 aggtaatgcg cgatgtcgcg gaacagccag gggcggccct gggcggcgcg gccgatcatg
      841 atgccgtccg cccccgtgag atcgaggacg gctcttgcct gcgccggcgt acggacatcg
      901 ccgttcgcga cgaccgggat gcgcgcccgc gacttcactt ccgcgatcgt gcggtattcg
      961 gcctgtccag cgaaggcgca ggcacgcgtg cgtccgtgaa cggccagcat ctgcacgccc
     1021 gccgcttctg caatggcggc gatgcgcagc gcattgcgcc gctccggaga cggcccggtg
     1081 cggatcttca gcgtgaccgg gaccggcacc gctgcaacca ccgcatcgag gatccggccg
     1141 acgagcgcct cgtcggcaag cagcgcggaa ccggcgctca cgttgcagac cttctttgcc
     1201 gggcagccca tgttgatgtc gatgatctgc gcgccctgat cgacattctg ccgggcggcg
     1261 tcggccagca tcagcggatc ggctccggca atctgcacgg agatcgggcc ggtctcgccg
     1321 cgatggtcga gccgcaggcg ggtcttgcgc gaggcgcgca gctccggacg cgccgatacc
     1381 atctcggata ccgccatcgc cgcgccgaat ttgcgcgcga gcgtccggaa cggtcggtcc
     1441 gtgattccag ccatcggagc aacgattact gcgttgcgca gaacgtgcgc gccaatcttc
     1501 atcaggcggc gtggcgttac gcgagcagga ttagggaaac ggattctagc ttagggcaac
     1561 gcggatgcgc catagatcat gcgccgcgcg aaaaagcgcc gcggcgcagg caagatatcg
     1621 agagcggtaa gcgctgcgcc gcgcaagctg gcggccagcc ggccggagcc gaggaatgcg
     1681 cgagccaggc cgtcggtgag acggatggct gcctgcgcat cgatgcgccg gcgcgaagca
     1741 tagcgggcga gcagcctgga atcgccggga tcgccggcgt tgcgcaaaat ctgcgccagc
     1801 tcccaggcgt cgcgcaggcc gacattgagt ccctggccgg cgacagggtg aagggtctgc
     1861 gcggcgtttc ctatatagac ctggcgctcg gccacgcgcg tgcgacgcac gcgcagggcg
     1921 agcggttgcg ccgagcgctc ctcgactgcg atgggcctgc ccgcgccgtg cggcgccgcc
     1981 gcgcacagcg cccgcaggaa cacctccgcg gagccgagcg cgagatcctg cgcgcgcgcc
     2041 ggcggcaggg tccatatgac ggccgagccg cgttgcaacg gcaggacggc gagcggccct
     2101 tcggacgtaa agcgctcatg cgcggtctcg gcctctccag actcgaag
//
LOCUS       DATDMP010000564         3439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1200029, whole genome shotgun sequence.
ACCESSION   DATDMP010000564 DATDMP010000000
VERSION     DATDMP010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200029"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="murD"
                     /locus_tag="VI195_09920"
     CDS             <1..965
                     /gene="murD"
                     /locus_tag="VI195_09920"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY6824746.1"
                     /translation="LLARMAARAGLAVRAGGNLGEPALDLIRDGTALYVLELSSFQLE
                     TVSHLECRAATVLNVSADHLDRYASIDDYAAAKARIFARCDTAVVNLDDPLVLAMPRA
                     VRRTLSFSLRAAIGADYAVASHRGDWWLTRAGRPLLPVAAMKIKGLHNAANALAALAL
                     GEALELPLPGMLTELTEFAGLPHRSQWVAEVRGVTYINDSKGTNVGATLAAVGGMPGP
                     LVVILGGDGKNQDFAPLAAAFRGKVRHAVLIGRDAPLVAGALAGVCPLETQRSLPEAV
                     QAAARAAQPGDTVLLSPACASLDMFRDYSERGAVFTAAVGELAA"
     gene            962..2170
                     /gene="ftsW"
                     /locus_tag="VI195_09925"
     CDS             962..2170
                     /gene="ftsW"
                     /locus_tag="VI195_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY6824747.1"
                     /translation="MSSTASLPLTGLVRSGGRSRTVHFDSVTVALTLAILLLGLVMVT
                     SASVSIASQQDHQPFYFLERQLLLSLIGAAGALAVFSVRTELLERVSGPLLALALLLL
                     VLVLVPGLGHAVNGSRRWLRILGGNFQVSELARVLVLVYLASYAVRRETELRQSFLGL
                     AKPLGLLAVACALLVAEPDLGAATVLFTAGFGLLFLAGARLRHMLAVAALAAGAFALA
                     AVSSPYRLRRLTTFLDPWADPFNSGFQLTQSLIAIGRGQWFGVGLGESVQKLFYLPEA
                     HTDFLFAVLAEELGLVGVIVTLALFVALIWRAFHIARLAQEAGLRFPALLAAGFGLWV
                     GIQTFINIGVNMGVLPTKGLTLPLMSYGRSSLIVALVWVGVVLRVCHEATRERRGAAT
                     VRGGTWRDDS"
     gene            2167..3252
                     /gene="murG"
                     /locus_tag="VI195_09930"
     CDS             2167..3252
                     /gene="murG"
                     /locus_tag="VI195_09930"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY6824748.1"
                     /translation="MSGPILIMAGGTGGHVFPALALARLLRSRSLEVVWLGTARGLES
                     RLIPAEGIALERLSVGGLRGKGVLAWLAAPVRLTVALAQALAVMRRHRPVAVVGLGGF
                     VTGPGGVAAWLTRRPLIIHEQNAIAGFTNRCLAHLARVVLEAFPGSFGAAVHARAIGN
                     PVRSDISALPPPPARFAGRAGPVRILVLGGSQGAARLNTVVPFALARLAGRIEFEVRH
                     QTGERWLEAGRASYAQAGVRAQLRPFIEDMGESYAWADLVICRAGALTISELAAAGVA
                     AVLVPFPGAVDDHQARNAQFLVRAGAAIVIADRELTPERLAGELERLGAGRGRLLAMA
                     ERARQLARPGAADELAASCLALARSAA"
     gene            3267..>3439
                     /locus_tag="VI195_09935"
     CDS             3267..>3439
                     /locus_tag="VI195_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003654096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY6824749.1"
                     /translation="MLPERPATERRVRPDRRDRMRRINTIHFVGIGGSGMSGIAEVLL
                     NLGYAVQGSDLRA"
BASE COUNT          386 a         1248 c         1252 g          553 t
ORIGIN      
        1 cactgctggc gcgcatggcg gcgcgcgccg ggctcgcggt gcgtgccggc ggcaatctcg
       61 gcgaaccggc actcgacctc atccgggacg gcaccgcgct ctacgtgctc gagctctcga
      121 gctttcagct cgaaacggtg agccacctcg agtgccgcgc cgccaccgtg ctcaacgtga
      181 gtgccgacca cctcgaccgt tacgcttcga tcgacgacta cgcggcggcc aaggcgcgca
      241 tcttcgcgcg ctgcgacacc gcggtggtga atctcgatga cccgctggtg cttgccatgc
      301 cgcgcgccgt gcggcgcacc ctgagcttct cgctgcgtgc cgcgatcggc gccgactacg
      361 cggtcgcgag ccaccggggc gactggtggc tgacgcgcgc cggccggccg ctgctgccgg
      421 tcgccgccat gaagatcaag ggactgcaca acgccgccaa cgccctcgcc gcactcgcgc
      481 tcggggaggc actcgagctc ccgctcccgg gcatgctcac cgagctcacc gagttcgccg
      541 gcctgccgca ccgctcgcaa tgggtcgccg aggttcgtgg cgtcacttat atcaacgact
      601 ccaagggcac caacgtcggc gcgacgctcg cggcggtcgg cggcatgccc ggtccgctgg
      661 tggtgatcct ggggggcgac ggcaagaacc aggacttcgc gccgctcgcg gcggcttttc
      721 gcggcaaggt gcgccacgcg gtgctcatcg ggcgggacgc gccgctggtc gcaggtgcgc
      781 tcgccggggt ctgcccgctc gagacccagc gctcgcttcc cgaggcggtg caggctgcgg
      841 cgcgtgccgc acagcccggc gacacggtac tgctgtcgcc ggcgtgcgcc agcctcgaca
      901 tgttccgcga ctacagcgag cgcggcgcgg tgttcaccgc ggcggtcggg gagctcgcgg
      961 catgagcagc acggcgagcc tcccgctgac gggattggtg cgttccggcg gtcgctcccg
     1021 cacggtgcac ttcgacagcg tcaccgtggc gctcacgctc gccatcctgc tgctcggact
     1081 cgtcatggtg acctcggcct cggtgtcgat cgccagtcag caggatcacc agccgttcta
     1141 ttttctcgag cgccagctgc tactcagcct gatcggcgcc gccggcgccc tcgcagtgtt
     1201 ctcggtgcgt accgagctgc tcgagcgcgt ctcgggcccg ctgctcgcgc tcgcgctcct
     1261 gttgctggtg ctggtactgg tgccgggtct cgggcacgcg gtgaacggca gccgccgctg
     1321 gctgcggatc ctcgggggca acttccaggt ctcggagctc gcgcgtgtgc tggtgctcgt
     1381 gtacctggcg agctacgcgg tgcggcgcga gacggagctg cgccagagct tcctcgggct
     1441 ggccaagccg ctcggcctgc tcgccgtcgc ctgcgcgctg ctggtcgcgg agccggatct
     1501 gggagccgcc acggtgctgt tcaccgccgg cttcgggctg ctgttcctcg ccggcgcgcg
     1561 gctgcgtcac atgctcgccg tcgcggcact cgcggccggc gcctttgcgc tcgccgccgt
     1621 cagctccccc tatcggctgc gccgcctcac gaccttcctc gatccctggg cggatccctt
     1681 caacagcggc ttccagctca cccagtcgct gatcgccatc ggccgcggcc agtggttcgg
     1741 ggtggggctc ggcgagagtg tgcagaagct gttctatctg cccgaggcgc acaccgattt
     1801 cctgttcgcg gtgctggcgg aggaactggg gctggtgggc gtgatcgtga cgctggcgct
     1861 gttcgtggcg ctcatctggc gcgccttcca catcgcccgg ctggcgcagg aggccggact
     1921 gcgcttcccg gcgctgctcg ccgcgggctt cggcctgtgg gtgggcatcc agactttcat
     1981 caacatcggc gtcaacatgg gagtgctgcc caccaagggg ctgacgctgc cgctgatgag
     2041 ctacgggcgg tcgagcctga tcgtggcgct ggtatgggtg ggagtggtac tgcgcgtgtg
     2101 ccacgaggcg acgcgcgagc ggcgcggcgc ggccacggtg cgcggcggca cgtggcggga
     2161 cgattcgtga gcggcccgat cctcatcatg gcgggcggca ccggcggaca cgtgtttccg
     2221 gcgctcgcgc tcgcgcggct gctgcgctcg aggtcgctcg aggtggtgtg gctcggaacc
     2281 gcccgcggac tcgagtcgcg gctcattccg gccgaaggca ttgcgctcga gcggctctcg
     2341 gtcggcgggc tgcgcggcaa gggtgtgctc gcctggctcg ccgctccggt acgcctcact
     2401 gtcgcgctgg cacaggcgct cgccgtcatg cgccgccacc ggccggtggc ggtggtggga
     2461 ctcggcggct tcgtcaccgg gccgggcgga gtcgccgcct ggctcacgcg ccggccgctc
     2521 atcatccatg agcagaacgc catcgccggc ttcaccaacc gctgcctcgc gcatctggcg
     2581 cgcgtggtgc tcgaggcctt tccgggcagc ttcggcgcgg cggtgcacgc gcgagccatc
     2641 ggcaacccgg tgcgcagcga catcagcgcg ctgccgccgc cgccggcgcg ctttgccggc
     2701 cgtgccggcc cggtgcggat cctggtgctc ggcggcagcc agggcgcggc gcgcctgaac
     2761 accgtggtcc cctttgcgct cgcgcggctc gcgggccgga tcgagttcga ggtgcgccac
     2821 cagacgggcg agcgctggct cgaggccggg cgcgccagct acgctcaggc gggcgtgcgc
     2881 gcgcagctgc gccccttcat cgaggacatg ggcgagtctt acgcctgggc ggacctggtc
     2941 atctgccgcg ccggggcgct caccatttcc gagctggccg cggccggggt ggcggcggtg
     3001 ctggtgccgt ttcccggcgc ggtcgacgac catcaggccc gcaatgccca gttcctggtg
     3061 cgcgccgggg cggcgatcgt gatcgccgat cgtgagctca ctcccgagcg gctcgccggc
     3121 gagctcgagc gcctcggcgc ggggcgcggc aggctgctcg ccatggccga gcgtgcccgg
     3181 cagctcgccc ggccgggggc cgccgacgag ctcgccgcct cgtgcctggc gctggcgcgg
     3241 agcgccgcat gatcatcgag cggcgcatgc tgcccgagcg tcccgcaacc gagcggcgcg
     3301 tgcgccccga ccggcgcgac cgcatgcgcc gcatcaacac cattcacttc gtcggcatcg
     3361 gcggcagcgg catgagcggg atcgccgagg tgctcctcaa cctcggctac gccgtgcagg
     3421 gctccgacct gcgggcgag
//
LOCUS       DATDMP010000565         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1533403, whole genome shotgun sequence.
ACCESSION   DATDMP010000565 DATDMP010000000
VERSION     DATDMP010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533403"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VI195_09940"
     CDS             complement(<1..430)
                     /locus_tag="VI195_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6824750.1"
                     /translation="MSLRKPALDPHTVEAVNSSGYPEPYRSRVLPRAKRRIGDALGLT
                     HIGINYTVLAPGKESSMRHWHTHEDEFVFVLQGELVLRTESGEQRLTAGMCAGFPAGS
                     EDGHQLINRSGLDAVYLEISNRDRADGAHYSDPEVDLAWSP"
     gene            493..>2559
                     /locus_tag="VI195_09945"
     CDS             493..>2559
                     /locus_tag="VI195_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824751.1"
                     /translation="MPETFADVGHCVDAVLRRVGPRVVLALPLGIGKPTPLANEFYRR
                     ACRDPSLELTVLTALSLLKPQARSALEARLLEPLTARVFGSYVEPEYARDLRADRVPA
                     NVRIIEFYLAPGALLHSGHAQRHYLSANYTHVARDALARGVNVIAQLLARRVVNGELQ
                     LSFGSNPDVSVDLLPLVASARHAGRDIVLVGETHAQMPFMTGHAQLDPRQLDFLIDDP
                     RYDYDLFGPPHTAIGTVEHAIGLQVSGLLRDGGTLQVGIGELGAAVVYALLLRHQQNA
                     SWRAARAALAGAHDAALIQQEGGVEPFAAGLFASSEMFVDYLLELYRAGILRRQVYDC
                     LPLERLLASASIGERFDERILERLAAAGAGPRLDAQEFATLQHYGVFREEIEFAAGRV
                     RLPGGEWLAADLADAAGRARLAACLGRGLRNGQVLHAGFFLGPRGFYAALRELPESER
                     ARFGMRGVGFVNQLYGADQELRVLQRRAARFVNTSMMVTLLGAAVSDTLADGRVVSGV
                     GGQYNFVAMAHALPGARSIVCVRATRTRHGRTASNIIWSEGNETIPRHLRDIVVTEYG
                     VADLRGRTDEECIAALLNISDSRFQESLLARARAAGKLRAGHRIPEGFRDNTPARLGR
                     ALQPQRARGYFSEYPFGTDLTGEEVVLARALSYLESRTGSAPARLLTAARALVRGSAA
                     PRHRTAL"
BASE COUNT          355 a          914 c          876 g          414 t
ORIGIN      
        1 acgggctcca ggcgagatcc acttcggggt cactgtagtg cgccccatcg gcgcggtcgc
       61 ggttgctgat ctcgagatac accgcgtcga ggccgctgcg attgatgagc tgatggccgt
      121 cctcgctgcc cgcggggaag cctgcgcaca tgccggccgt gagccgttgc tcgccgctct
      181 cggtgcgcag caccagctcg ccctggagca cgaacacgaa ctcatcctcg tgcgtgtgcc
      241 agtggcgcat ggaagattcc ttgccgggag cgagtaccgt gtagttgatg ccgatgtggg
      301 tgagccccag ggcatctccg atgcggcgtt tcgctcttgg caacacgcgc gagcgatagg
      361 gctccgggta accgctgcta ttgaccgctt cgacggtgtg tggatcgagg gccggcttgc
      421 gcaatgacat ggctgactcc cggacgaggt tgacggatac cgtagccctc ggggagcatt
      481 gccacaagct gcatgcccga gacattcgct gacgtcggcc actgcgtcga tgccgtgctg
      541 cgccgtgtgg ggccgcgggt ggtgctcgcg ctgccgctcg gcatcggcaa gccgaccccg
      601 ctcgcgaacg agttctaccg ccgcgcctgc cgcgacccaa gcctcgagct caccgtgctc
      661 accgcgctct cgctgctcaa gccgcaggca cgctcggcgc tcgaggcgcg cttgctggag
      721 ccgctcactg cacgggtgtt cggcagttac gtcgagccgg agtacgcgcg cgacctgcgc
      781 gcggaccgag tgcccgccaa cgtgcgcatc atcgagttct acctcgcgcc gggcgccttg
      841 ctgcactctg gccacgcgca gcggcactac ctgagcgcca actacaccca cgtggcccgc
      901 gatgcgctcg cgcgcggcgt gaatgtgatc gcccagctgc tcgcgcgacg cgtggtgaac
      961 ggcgagctgc agctgagctt cggctccaac cccgatgtga gcgtcgacct gctgccactg
     1021 gtcgcgtcgg cgcggcacgc cggccgcgat atcgtgctgg tcggcgagac gcacgcgcag
     1081 atgccgttca tgaccgggca cgcacagctc gaccccaggc agctcgactt cctcatcgac
     1141 gatccgcgtt acgactatga cctgttcggt ccgccgcaca ccgcgatcgg caccgtcgag
     1201 catgccatcg gcctgcaggt gagcgggctg ctgcgcgatg gcggcacgct gcaggtcggc
     1261 atcggcgagc tcggcgccgc ggtggtgtat gcgttgctgc tgcgtcatca gcagaacgcc
     1321 agctggcgcg ccgcgcgcgc cgcgctcgcc ggtgcgcacg acgcggcgct catccagcag
     1381 gaaggtggtg tcgagccctt cgcggccggc ctgttcgcct cctcggagat gttcgtcgat
     1441 tacctgctcg agctgtaccg ggcgggcatc ctgcggcgtc aggtgtatga ctgcctgcca
     1501 ctcgagcggc tgctcgcgtc cgcaagcatc ggcgagcgct tcgatgagcg cattctcgag
     1561 cgtctcgccg ccgccggtgc cggcccgcgg ctcgacgcgc aggagttcgc cacgctgcag
     1621 cactacggcg tgtttcgcga agaaatcgag ttcgctgccg ggcgcgtacg cctaccgggc
     1681 ggggagtggc tggcggccga tctcgccgac gccgccggcc gcgcgcggct cgccgcctgc
     1741 ctcggccgtg gcctgcgcaa cggccaggtg ctgcacgccg gcttcttcct cggcccacgc
     1801 ggcttctacg cagcgctgcg cgagctgccg gagagcgaac gcgcgcgctt cggcatgcgc
     1861 ggcgtcggct tcgtcaatca gctctacggc gcagaccagg agctgcgcgt gctgcagcgg
     1921 cgcgcggcgc gcttcgtcaa taccagcatg atggtgacgc tgctcggtgc ggcggtatcc
     1981 gatacgctgg cagacggccg cgtggtgagc ggggtcggcg gccaatacaa cttcgtcgcc
     2041 atggcgcatg ccctgccggg ggcgcgctcg atcgtgtgcg tgcgcgcgac gcgcacccgg
     2101 cacggccgta ccgcatccaa catcatctgg agcgagggta acgagacgat tccgcgccac
     2161 ctgcgcgaca tcgtcgtgac cgaatacggc gtcgccgacc tgcgcggccg caccgatgag
     2221 gagtgcatcg ccgcgctgct gaacatcagc gactcgcgtt tccaggagtc gctgctcgcc
     2281 cgcgcccgcg ccgccggcaa gctgcgcgcc ggacaccgca ttcccgaagg cttccgcgac
     2341 aacactcccg cgcggctcgg gcgcgcgctg caaccccagc gcgcgcgcgg ttacttcagc
     2401 gaatatccct tcggcacgga tctcaccggc gaggaggtcg tgctggcacg cgcgctgtcg
     2461 tacctcgaaa gccgtaccgg gagcgctccg gcgcggctgc tcaccgccgc gcgcgcgctc
     2521 gtccgcggct ccgcagcgcc gcgacatcgg acggcgctc
//
LOCUS       DATDMP010000566         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_978404, whole genome shotgun sequence.
ACCESSION   DATDMP010000566 DATDMP010000000
VERSION     DATDMP010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978404"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VI195_09950"
     CDS             <1..547
                     /locus_tag="VI195_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6824752.1"
                     /translation="GYGLGLSIVQRLVRLLNLRLEVTSELGKGSVFSLLLSRGRSQGV
                     SEQPRSATAGHAATAGRPRVLLVEDDAGVRDATRMLLSVEGYRVSAVASLAEALRSAQ
                     QEGAPDLLITDYHLGEGELGTQVIAALRAHLGTELKAVLVTGDTSPVVKHMPNDPRLR
                     IAAKPIDAEQLLKVLEQLLSS"
     gene            complement(601..1227)
                     /locus_tag="VI195_09955"
     CDS             complement(601..1227)
                     /locus_tag="VI195_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6824753.1"
                     /translation="MQPATPTVFVVDDDEGVRNSLRFLLKSVGLTTRALGSASEFLEI
                     YKPNQPGCLVLDVRMPGMSGIELQQQLNVRGATIPVIFITGHGDIPMAVEAMQHGAFD
                     FLQKPFRDQDLIDRIQRALERDARNRTALTQHARIRERLESLTPREREVLTLMTRGKP
                     NKVMAAELGVSQRTVEIHRARVMEKRGADSLAHLVRMVMDLEAEQPAG"
     gene            complement(1233..1952)
                     /locus_tag="VI195_09960"
     CDS             complement(1233..1952)
                     /locus_tag="VI195_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824754.1"
                     /translation="MALHGRLLNVSRMATIGEMAAGVAHELNQPLTAIANYAHACERL
                     LSRPQSDSESLREALRQITAQTSRAAEILRRLRALARSQQAEHAPASLNTLVTELYEL
                     LQTDAIVHGVELTLDLAPRLPEVRVDPGQIQQALLNLVRNSLDALAGRAASGGHITIR
                     TALTAEGEAQLSVIDDGPGVSPQAMTRIFDPFFSTKENGTGLGLAITHTIARSHGGSV
                     SNRPNVPSGACFDILLPTQPR"
     gene            complement(2185..>2496)
                     /locus_tag="VI195_09965"
     CDS             complement(2185..>2496)
                     /locus_tag="VI195_09965"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824755.1"
                     /translation="YTTIVDATFARTEDRERFRELAASLGVNLCIVYCHAPQNILRKR
                     IGERGRRGKDPSEANLEVLRWQEAHFLPPAPQEASVVLDAAKLTAPQLVRRIAAVAAR
                     S"
BASE COUNT          393 a          808 c          867 g          428 t
ORIGIN      
        1 aggctacgga ctcggcctca gcatcgtgca gcggctggtg aggctcctca acctgcgcct
       61 ggaagtgact tcggaactcg gcaagggatc ggtgttctcg ctgttgctct cgcgcggccg
      121 atcacaggga gtcagcgagc agccgcgctc ggccactgcc gggcacgccg ctaccgcggg
      181 ccgtccccgg gtcctgttgg tggaggacga tgcgggcgtg cgcgatgcga cacgcatgct
      241 gttgagcgtc gagggctacc gggtgagcgc cgtggcctcg ctcgccgagg cgctgcgcag
      301 cgcgcagcag gagggcgccc cggacctgct gatcaccgac tatcatctgg gcgagggtga
      361 gctcggcacg caggtcatcg ccgctctgcg tgcgcacctg ggcaccgagc tcaaggcggt
      421 gctggtcacc ggcgacacct ccccggtggt gaagcacatg ccgaacgatc cgcggctgcg
      481 gattgcggcc aagccgattg acgcggagca gctgctgaaa gtactcgaac agctgctgag
      541 cagctgagcc agagcgcgtc gaggtacagg cgcgtgcgcc gcgccgcccg cgcggtgcgc
      601 tcagccagcg ggctgctcgg cttccaggtc catcaccatg cgcaccaggt gcgcgagcga
      661 gtcggcgccg cgtttctcca tgacgcgcgc ccgatgaatc tccacggtgc gctggctcac
      721 tccgagctcc gcggccatta ccttgttggg cttgccgcgg gtcatcagcg tgagcacttc
      781 ccgctcgcgc ggggtgagcg actcgaggcg ctcacggatg cgtgcatgct gcgtgagggc
      841 cgtgcggttc ctggcatcgc gctcgagcgc acgctggatg cggtcgatga ggtcctggtc
      901 gcgaaagggc ttctgcagga agtcgaacgc gccgtgctgc atcgcctcga ccgccattgg
      961 gatatcgcca tggccggtga tgaaaatgac cggaatggtt gcgccgcgga cattcaactg
     1021 ctgctgcagc tcgataccgc tcatgccggg catgcgcaca tcgagcacca ggcagccggg
     1081 ctgattgggc ttgtagatct cgagaaactc actcgccgag ccgagcgcgc gggtcgtgag
     1141 ccccacggac ttcagcagga agcgcagcga gttgcgcact ccttcatcgt cgtcgacgac
     1201 aaacacggtg ggcgtggcgg gctgcatggg cactaccggg gctgcgtggg tagaaggata
     1261 tcgaagcaag cgccgctcgg cacgttgggg cggttgctca ccgagccacc gtgactgcgc
     1321 gcaatcgtgt gggtgatcgc aagtccgagg ccggtaccgt tctccttggt ggagaaaaac
     1381 gggtcgaaga tgcgcgtcat cgcctgcggc gagacaccgg gcccgtcgtc gatgacactc
     1441 aactgcgctt cgccctcggc ggtgagcgca gtgcggatcg tgatatgccc accgctcgca
     1501 gcccggccag cgagcgcatc gaggctgttg cgcacgagat tcaggagcgc ctgctggatc
     1561 tgccccgggt cgacccgcac ttcgggaagc cgcggggcga ggtcgagcgt caactccacc
     1621 ccatggacga tcgcatcggt ttgtagcagc tcgtacagct ccgtcaccag agtgttgaga
     1681 ctcgccggcg catgctcggc ctgctggctg cgggcgagcg cgcgcaggcg ccgcaggatt
     1741 tcggcggcgc gcgaggtctg cgcggtaatt tgccgcaaag cctcgcgcag cgactcgctg
     1801 tcgctctgcg gtcgcgacag cagccgctcg caggcgtgag cgtagttggc gatggccgtg
     1861 agtggctgat tgagctcgtg cgccactccg gcggccatct caccgatggt cgccatgcgc
     1921 gagacattca gcaaccggcc gtggagtgcc acgggagcct cccgcggcgc cagcgcgtcg
     1981 ggctggagcg tcccctcggt ccgatgggct gctgcggctc ggtcctcgat gatcatttct
     2041 gccgtgacaa ccatacaagt ctgcacaccc aagcatccac ggtttgttac tgcgcgtctt
     2101 tacgtagttg tcacagagtc ttatcttggc accctgtgcg gcccgcggct ccctgtcgat
     2161 aagtagttac cgagggtcgc tgcgctagct tcgcgccgcg acagccgcaa tgcggcgcac
     2221 gagctgcggc gcagtgagct tcgccgcatc gagaacgacg ctcgcctcct gcggcgcggg
     2281 cgggaggaag tgcgcctcct gccaacgcaa cacctcgagg ttggcttccg agggatcctt
     2341 gccgcggcgg ccgcgctccc cgatgcgctt gcgcaggatg ttctgcggcg catggcaata
     2401 gacgatgcac aggttcaccc cgagggaagc ggccagttcg cggaaacgct cccggtcctc
     2461 ggtgcgggcg aaggtcgcat cgacgatcgt ggtgta
//
LOCUS       DATDMP010000567         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_645979, whole genome shotgun sequence.
ACCESSION   DATDMP010000567 DATDMP010000000
VERSION     DATDMP010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645979"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VI195_09970"
     CDS             <1..347
                     /locus_tag="VI195_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6824756.1"
                     /translation="FQKLKIIASHGGGTLPYLIGRMDQCYEHIPACRTKTREKPSLHA
                     RRIYADSVVFTDEALAMAVSVFGSGNVLYGSDYPHTIGDPIGCLARVDRLPGGARERV
                     RGGNALRIFSFS"
     gene            complement(344..688)
                     /locus_tag="VI195_09975"
     CDS             complement(344..688)
                     /locus_tag="VI195_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824757.1"
                     /translation="MRGAILALAVLLPVAAAAHPHSASECREGGDFIRNAALSRDGGA
                     TREFFVGRLEEDLQTIKAFPPALRWFVRDADDEQFLRAEVEAVFDTPQSSEQHRDGFL
                     ARCARRAGASDA"
     gene            727..1500
                     /locus_tag="VI195_09980"
     CDS             727..1500
                     /locus_tag="VI195_09980"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY6824758.1"
                     /translation="MTIYFEGAARKARDLPVSESERAARVQLAACYRVFHHLGWTEMI
                     FNHVTLRVPGAEKLFLINPFGLHYSEITASSLVLIDLEGNPVREAKWPVNRAGFVIHS
                     AIHGSLEHAHCVMHTHTTTGMAVACLKDGLSPTNFYAAQLHGGVAYHDFEGITVEEGE
                     KARLVASIGAKRAVILRNHGLLAWGPSLPEAFMTLWTLQRACDVQIASSCAGPLNPIR
                     NDVFAQTVRESGPGEKRTCEDVFAAMQRLADAKDPSYRD"
     gene            complement(1488..>2748)
                     /locus_tag="VI195_09985"
     CDS             complement(1488..>2748)
                     /locus_tag="VI195_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY6824759.1"
                     /translation="AVGFNAQIEIDYQALEIDRGDVFVLATDGVYENVDAAFVARAIR
                     EARGGLHDPARGDLDGAARAVVEEAHRRGSADNLTVQIVCVEDVPQDGNGLRGQLARL
                     APPPLLQARDEIDGYCIEREIHASARSHIYLATDLETGGLVALKMPATDLQSDPELIE
                     RFMLEEWIARRLNSPHVLKPFPRTRERRFLYVAMEYVEGQTLAQWMIDNPRPALETVR
                     GIVEQVARGLQAFHRMEMLHQDLRPQNIMVDRTGTVKIIDFGSASVAGVDEQARAAEP
                     SRILGTLQYTAPEYFIGDAGSERADLYSLGVIAYQMLSGRLPYGAAAAQVRTRSALRR
                     LEYESVLDEQRDIPRWVDAALRMAVQPEPRERYEALSEFVYDLRHPNAELLRRTPLIE
                     RNPVTFWKCVSALLMAALLVLLFIQSR"
BASE COUNT          437 a          957 c          921 g          433 t
ORIGIN      
        1 gcttccagaa gctgaagatc atcgccagcc acggcggcgg cacgctgccc tacctgatcg
       61 gccgcatgga ccagtgctac gagcacatcc ccgcctgccg caccaagacg cgggaaaagc
      121 cgagcctgca tgcgcgccgc atctacgccg attcggtggt cttcaccgac gaggcgctcg
      181 ccatggcggt cagcgtcttc ggctcgggaa acgtgctcta cggctcggac tatccccata
      241 ccatcggcga cccgatcggc tgcctcgcgc gcgttgaccg gctgcctggc ggggcgcgcg
      301 agcgcgtgcg cggcggcaac gcgctcagga tcttcagctt cagctaggcg tcggacgcgc
      361 cggcgcgccg cgcacagcgc gcgaggaagc cgtcgcggtg ctgctcgctg ctttgtggcg
      421 tgtcgaacac ggcctcgacc tcggcgcgca ggaattgctc gtcgtccgca tcgcgcacga
      481 accagcgcag cgcgggcggg aaggccttga tggtttgcag gtcttcctcg agccgcccta
      541 cgaagaactc gcgcgtggcg ccgccgtcgc gcgacaacgc cgcattgcgg atgaagtcgc
      601 cgccctcgcg gcactcgctt gcactatggg gatgggcggc cgctgccacg ggcaggagca
      661 ccgccaacgc aaggattgcg cctcgcatgc gggggtagac tactcctcct cgcccggagc
      721 gcaaagatga ccatttattt cgagggcgcc gcccgcaagg cgcgcgacct gccggtttcg
      781 gagtccgagc gcgccgcgcg cgtgcagctc gccgcctgct accgcgtttt tcaccatctc
      841 ggctggacgg agatgatctt caatcacgtc acgctgcgcg tgccgggagc ggagaagctt
      901 ttcctcatca accccttcgg cctgcactac agcgaaatca cggcgtcgtc gctggtgctg
      961 atcgacctcg agggcaaccc ggtacgcgag gcgaaatggc ccgtgaaccg ggctggcttc
     1021 gtgatccaca gcgcgatcca cggcagcctg gagcacgcgc actgcgtgat gcacacgcac
     1081 actacgaccg gcatggcggt ggcttgcctg aaggacgggc tctcgcccac caacttctac
     1141 gccgcgcagc tgcacggcgg cgtggcctac cacgacttcg agggcatcac ggtcgaagaa
     1201 ggcgagaagg cacggctggt cgccagcatc ggcgcgaagc gagcggtgat cctgcgcaac
     1261 cacggactgc tcgcctgggg accgtccctg ccggaagcgt tcatgacgct gtggacgctg
     1321 cagcgcgcct gcgacgtgca gatcgcctcc agctgcgccg gaccgctgaa tcccatcagg
     1381 aacgacgtgt ttgcccagac cgtgcgcgaa tcgggccccg gcgagaagcg cacctgcgag
     1441 gacgtgttcg cggcgatgca gcgcctcgca gacgcgaagg atccgtccta ccgcgattga
     1501 atgaagagca ggaccagcag cgccgccatc aggagcgctg acacgcattt ccagaaagtc
     1561 acgggattgc gctcgatcag cggcgtcctg cgcaggagct ccgcgttggg gtggcgcagg
     1621 tcgtagacga attcagacag cgcctcgtag cgctcgcgcg gctcgggctg gaccgccatc
     1681 ctgagcgccg catcgaccca gcgcgggata tcgcgctgct cgtcgagcac cgactcgtat
     1741 tccagcctgc gcagcgccga ccgcgtccgc acctgggcgg ccgccgcgcc gtacggcagg
     1801 cggcccgaca gcatctggta ggcgatgacg cccagcgaat agagatccgc gcgctcgctg
     1861 ccggcgtcgc cgatgaagta ttccggcgcg gtgtactgca gcgtgccgag gatgcgggac
     1921 ggctccgccg cccgcgcctg ctcgtcgacc cctgcgacgc tggccgaacc gaagtcgatg
     1981 atcttcaccg tgccggtccg gtcgaccatg atgttctggg gacgcaggtc ctggtgcagc
     2041 atctccatgc ggtggaacgc ctgcaggccg cgtgcgacct gctcgacgat gccgcgcacg
     2101 gtctcgagcg ccggccgcgg gttgtcgatc atccactgcg ccagggtctg gccttccacg
     2161 tattccatcg cgacatacag gaagcggcgc tcgcgcgtgc gtggaaaggg cttcagcacg
     2221 tgcgggctat tcaggcgccg cgcgatccac tcctccagca tgaaccgctc gatcagctcg
     2281 gggtcgctct gcaggtcggt ggcgggcatt ttcagcgcga ccagcccgcc ggtctccaga
     2341 tcggtcgcca ggtagatgtg gctgcgcgcg gaggcatgga tctcgcgctc gatgcagtag
     2401 ccatcgatct cgtcgcgtgc ctggagcagc ggcggcggcg cgagccgagc cagctgcccg
     2461 cgcagcccgt tgccgtcctg cggcacgtcc tcgacgcaga cgatctgcac ggtcaggttg
     2521 tccgcactgc cgcggcggtg cgcttcctcc accacggcgc gcgcagcgcc gtccaggtcc
     2581 ccgcgcgccg gatcgtgaag gccgccgcgc gcctcccgga ttgcccgtgc gacgaaggcg
     2641 gcatcgacgt tctcgtagac gccgtcggtg gcgagcacga acacgtcgcc gcgatcgatt
     2701 tcgagcgcct ggtagtcgat ctcgatctgc gcgttgaagc ctacggcg
//
LOCUS       DATDMP010000568         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1756974, whole genome shotgun sequence.
ACCESSION   DATDMP010000568 DATDMP010000000
VERSION     DATDMP010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756974"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VI195_09990"
     CDS             <1..214
                     /locus_tag="VI195_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824760.1"
                     /translation="HEYWPLLVTDGALQAGPPEAHSATVFNVESFFGWTIGTYALIEA
                     LGHARAASAQSSSGVAASRDSRSHGA"
     gene            complement(225..2426)
                     /locus_tag="VI195_09995"
     CDS             complement(225..2426)
                     /locus_tag="VI195_09995"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY6824761.1"
                     /translation="MRALERPAPSRRGFLLGGGALIVSFSLPLRLAMAQQPAEKKLPG
                     SLQRTARLDSWIRIDDRGAITVFTGKCELGQGIKTALMQVAAEELAVAAESIHLVTAD
                     TALTPNEGYTAGSHSMQDSGTAIRYAAAQARSLLIDAAAARLDVPRERLKAQDGAVVA
                     DDGRRLGFGALVKDELLHVAADGQSALTDAAHYRLIGMRVPRVDIPAKVSGGGAFVQD
                     LRLPGMVHARVVRPPSYHATLVRLDTTPIAKLPGVRTVHRDGNYLAVIAEHEYQAVVA
                     MQALAGAATWNETATLPEPDKIFDWLASQPAEQVPVLAGSGRLTGGTRILAATYRRAY
                     QMHGSIGPSCAVAQFESDQLTVWTHSQGVFPLRAAIAQLVQLPTERVRCVQIDGSGCY
                     GHNGADDAAADAALLAMALPGHPIRVQWTREQEHAWEPYGSAMITKVRAALGAQGRIS
                     GWDYSVRSCTHATRAGPAGNLLPAWLLAHPSTPPPPQPLPLPEGGGHRNAAPLYSIPD
                     ARVVYEFVSAMPLRVSALRSLGAYMNVFSIESFMDELAQAAKADPVDFRLRHVDDPRA
                     RAVIKVAAEHFGWADYHTRQDHGRGFAFARYKNLAAYLAVAAEVQVDRQRRRLRLKRA
                     VAAVDCGEAVNPDGIENQIAGGIIQSASWTLHERVAFDRMRITSRDWNSYPILRFSEI
                     PDEIEVRVLNQPGTPFLGTGEAAQGPTAAAIANAIADATGTRLRDIPLTLP"
     gene            complement(2423..2914)
                     /locus_tag="VI195_10000"
     CDS             complement(2423..2914)
                     /locus_tag="VI195_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY6824762.1"
                     /translation="MITLTVNGKVHQVEVDPETPLLYVLRDELALDGTKFGCGLGQCG
                     ACTVLVEGVAVNSCVTPVSTFTGRRVVTVEGLGTAGKLGLLQQAFIEEQAAQCGYCIA
                     GMIMRAQGLLDHNPAPSDTEIRRHMEPNLCRCGTHVRIFRAIRRASAALKHNATPPRE
                     AGK"
BASE COUNT          538 a         1026 c         1060 g          570 t
ORIGIN      
        1 tcatgaatac tggccgttgc ttgtgaccga tggtgcactt caggctggcc caccggaggc
       61 gcacagcgcc acggtgttca acgtcgaatc cttcttcgga tggacgatcg gcacctacgc
      121 gctcatcgag gcgctcggcc acgcccgagc tgcaagcgcg caaagttcga gtggtgtggc
      181 agcgtcgcgg gattcgcggt ctcacggcgc gtaggcgcgg agagtcacgg cagcgtcagc
      241 ggtatgtccc gaaggcgagt accggttgcg tcggcaatgg cattggctat cgccgcagcg
      301 gtcggcccct gcgccgcttc acccgtgccg aggaaaggcg tgcccggctg gttgagtacg
      361 cgaacctcga tctcatccgg aatttcgctg aagcgcagaa tggggtagct gttccagtcg
      421 cggctcgtga tccgcatgcg atcgaacgca acgcgctcat gcagcgtcca gcttgccgac
      481 tggatgatcc cgccggcaat ctggttctcg atgccatccg ggttcaccgc ctcaccacaa
      541 tcgaccgctg cgaccgcacg tttgagtcgc aggcggcgtc gttgccggtc gacctggact
      601 tcggcggcca ccgccagata tgcggcgagg tttttgtatc gcgcgaaggc aaacccgcga
      661 ccgtggtcct gccgcgtgtg gtaatcggcc cagccgaaat gctcggcggc cactttgatg
      721 accgctcgag cccgcggatc atcgacatga cggagccgaa aatcgaccgg atcggccttc
      781 gctgcctgcg ccagctcgtc catgaagctc tcgatcgaga agacgttcat gtatgcgccc
      841 aggctgcgca gtgccgaaac gcgtagcggc atcgcggaga cgaactcgta caccacgcgc
      901 gcatcgggga ttgaatagag tggcgcggcg ttgcggtggc cacctccttc gggcagagga
      961 agcggttgcg gcggtggcgg tgtgctcggg tgcgccagca gccaggccgg aaggagattg
     1021 ccggcaggac cggcacgcgt cgcatgtgtg cagctgcgca ccgagtagtc ccatccgctg
     1081 atgcgtcctt gtgcaccgag ggcggcacga accttcgtga tcatcgccga gccatagggc
     1141 tcccaggcat gctcctgttc ccgcgtccac tgcacacgta tcgggtgtcc ggggagcgcc
     1201 atcgccagga gcgccgcatc ggcggcggcg tcgtcagcgc cgttgtggcc ataacatccc
     1261 gagccatcga tctgaacgca gcgaaccctt tcggtcggta actgcaccag ttgggcgatc
     1321 gcagctcgca gcgggaagac tccctggcta tgagtccaga cggtgagctg gtcgctttcg
     1381 aattgggcca cggcgcacga tggcccaatg gagccatgca tctggtaggc gcggcgatac
     1441 gtcgccgcga ggatgcgcgt gccccccgtc agacggcccg atccggcgag aaccggtacc
     1501 tgctcggctg gttgcgacgc gagccaatcg aagatcttgt caggctccgg cagcgttgca
     1561 gtttcgttcc aggtggcagc gccggcgagc gcctgcatcg ccacaaccgc ctgatattcg
     1621 tgctcggcga tcacggccag ataattgccg tcacggtgca cggtccgtac gcccggcagc
     1681 tttgcgatcg gggtggtgtc gagacgcacc agcgtggcgt ggtaagaggg cggacgcacg
     1741 acgcgcgcat gcaccatccc ggggaggcgc aagtcttgga caaacgcgcc tccgccgctg
     1801 accttcgcgg gaatgtcgac acggggaacc cgcatgccaa tcagccggta gtgcgcggcg
     1861 tcggtgagag cggattggcc gtcggccgct acgtgcagca gctcgtcttt caccaacgct
     1921 ccgaagccga ggcggcgccc gtcgtccgcc acgaccgcgc catcctgtgc tttgagacgc
     1981 tcgcgcggga catccaagcg ggcagccgcc gcgtcgatca gcagcgagcg cgcctgggcg
     2041 gcggcatagc gaatcgccgt gccgctgtcc tgcatggaat ggctacccgc cgtgtaaccc
     2101 tcgttgggtg tgagtgcggt gtcggccgtg acgaggtgaa tcgactcagc cgcgaccgcc
     2161 agttcctcgg ccgcgacttg catgagtgct gttttgatcc cctggccgag ttcgcacttg
     2221 ccggtgaata ccgtgatcgc gccgcgatca tcgatccgaa tccaggaatc gagtctcgcg
     2281 gtgcgctgca ggctgccagg cagtttcttt tcggccggct gctgcgccat ggcgagtcgc
     2341 agtggaagcg agaaactcac gatcagcgcg cccccgccga ggagaaagcc acgtcgcgac
     2401 ggcgccggtc gctcgagcgc tctcacttgc ctgcctcacg cggtggcgtg gcattgtgtt
     2461 tcagtgccgc gctcgcccgc cgaatggcgc ggaagatccg cacgtgagtg ccgcaacgac
     2521 agagattggg ttccatgtgc ctgcggatct ctgtgtctga gggcgccgga ttgtgatcga
     2581 gcaacccctg cgcgcgcatg atcataccgg cgatgcagta gccgcattgc gcagcctgct
     2641 cttcgataaa agcctgctgc aatagtccca gcttgcctgc agtgccgagg ccctccacgg
     2701 ttacaacgcg ccgacctgtg aaggtgctca ccggggtcac gcaggaattg acggcgacgc
     2761 cctccaccag caccgtgcag gcgccgcact gacccaggcc gcagccaaat ttggtgccat
     2821 ccagcgcgag ctcgtcgcgc aggacgtaga ggagcggcgt ctcgggatcc acctcgacct
     2881 ggtgcacctt gccgttgact gtcagcgtga tcatttgtgc ttacgccggg tagcggagca
     2941 atgagcgctg tgaacgctgg aagcgcgtca gcgcgtccgt aaagcctgtc gaagctcagg
     3001 agcgcgcgac tcagaggtga gcccgagaca tcgcggcagt atacactcgc acaagtccgt
     3061 gcagtcgctc actacaacgt gggagatcgc gcaggccctg ggtctctgcg cagttgccgc
     3121 ttgtttcgtg ctctgcgtgc tggcggttcg gccgcgcgcg ggcgctatcg ctggctttcc
     3181 gatacgcggg cacg
//
LOCUS       DATDMP010000569         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_757627, whole genome shotgun sequence.
ACCESSION   DATDMP010000569 DATDMP010000000
VERSION     DATDMP010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757627"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VI195_10005"
     CDS             complement(<1..460)
                     /locus_tag="VI195_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY6824763.1"
                     /translation="MKVGELQVNRMGFGAMRVCGPNVWGPPKDRASAHKVFRRAIELG
                     VNFFDTADSYGPHVDEELIAEALHPYPKGLVIATKGGLLRPRPSAWDPDGRPEHLKRA
                     LDGSLRRLKLERIDLYQLHAPDGKVPFADSVGALADAQRAGKIRHVGVSNV"
     gene            complement(506..703)
                     /locus_tag="VI195_10010"
     CDS             complement(506..703)
                     /locus_tag="VI195_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824764.1"
                     /translation="MAPVREHGYRFDVEGDVADVSIQLHVLARAYFEACLALTRRAPA
                     GQHRLEMLGVTIEFEVPETPD"
     gene            complement(707..1054)
                     /locus_tag="VI195_10015"
     CDS             complement(707..1054)
                     /locus_tag="VI195_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824765.1"
                     /translation="MDDYTRKLKLTKTRIQSGVILDASGTIGNWPFRRTVSYHVGINC
                     DTIAVRDGQLVVQRRGRIVAIAESPDVPPSFVRMLRSALDALLHPRHARRTAADRLPG
                     VRWEDFFRAPPRK"
     gene            1171..2178
                     /locus_tag="VI195_10020"
     CDS             1171..2178
                     /locus_tag="VI195_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEY6824766.1"
                     /translation="METFLATTGRIFLRLLELHGIDADAFVREGGYAPEAFGDPEARL
                     PSSAVDVAARAAAARIADPAFALRAARCWHPSDLGALGYAWLASSTLRTALQRLERYG
                     RILGEKARFRLESGDRGLRLIFEHRRKDARVAALGTDFALSLVLDMCRMNFGASLRPL
                     EATLCRPCPADATPWRVFFGGDVRFGAARDSMLLSRRDADAALPVANRQIAGALDAIL
                     AQQLARLDRGNIAARCKAEVLERLASGESPEADIAKSLHMSRRTLQRKLAEADLTYQK
                     LVDDTRRDLALRYIEDARRSITEITFLLGFSGQSAFTRAFRRWAGVSPTEYRSGRRAQ
                     A"
     gene            2289..2468
                     /locus_tag="VI195_10025"
     CDS             2289..2468
                     /locus_tag="VI195_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824767.1"
                     /translation="MRDLAKLVLLGCSLFAAAFALWRLALVVALVMREFRGDRPGAEA
                     PDSSEQRTSSTSRSR"
     gene            2525..>2886
                     /locus_tag="VI195_10030"
     CDS             2525..>2886
                     /locus_tag="VI195_10030"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEY6824768.1"
                     /translation="MFAKAETVRAWVREAQRVVVLTGAGISTDSGIPDFRGPQGVWTR
                     NPMAEKLSNIHYYMADAEVRKAAWQARMDHTAWSAQPNAGHRAIAALEKSGKLHALIT
                     QNIDELHQQAGNSPEKVI"
BASE COUNT          437 a          975 c         1011 g          463 t
ORIGIN      
        1 tgacgttgga cacgccgaca tggcggatct tgccggcgcg ctgcgcatcg gccagcgcgc
       61 ccacggagtc cgcgaacggc actttgccgt cgggcgcatg cagctgatac agatcgatgc
      121 gctccagctt caggcgccgc aggctcccgt cgagggcgcg cttcaggtgc tcgggccggc
      181 cgtcgggatc ccaggccgag gggcgcggcc gcagcagacc gcccttggtg gcgatgacga
      241 ggcccttcgg atagggatgc agcgcctcgg cgatgagctc ctcgtccacg tgcgggccgt
      301 agctgtcggc ggtgtcgaag aagttcacgc cgagctcgat cgcgcgccgg aacaccttgt
      361 gcgcggaggc gcggtccttg ggcgggcccc agacgttcgg gccgcagacg cgcatcgcgc
      421 cgaagcccat gcggttgacc tgcagctcgc cgactttcat gccgtgcagt gtagtccacc
      481 ggccagccgg cgaccggccg tcgccctagt ccggagtctc cggcacttcg aattcgatgg
      541 tgactcccag catttcgagc cggtgctggc ccgccggcgc gcggcgcgtg agcgcgagac
      601 aggcctcgaa gtaggcgcgg gcgagcacgt gaagctggat gctgacgtcg gcgacgtcac
      661 cttcgacgtc gaatcgatag ccatgctctc tcacgggcgc cacggactat ttcctgggcg
      721 gtgcgcgaaa gaagtcttcc cacctgaccc caggcaagcg gtcggctgcg gtccgccgag
      781 cgtggcgcgg atgcagcagc gcgtccaatg cggagcgcag catgcgaacg aacgagggcg
      841 gaacgtccgg cgattcggca atcgcaacaa ttcgtccgcg gcgttgcacc acaagctggc
      901 cgtcccggac cgcaatcgtg tcgcaattga tgccgacgtg atagctgacc gtgcggcgga
      961 atggccaatt cccgatcgtg ccgctcgcgt cgagaatcac gcctgattgg atgcgcgtct
     1021 tcgtgagctt caacttcctt gtgtagtcgt ccatgaattc gaatgtcctc gcaagagttc
     1081 gcggaccgca ctgtcgcaac gccgcgccgc ctgcgcttgc ccgtgcgcgc cagccgtttg
     1141 cccggcggtg ccataatcgg tgcgggcacc atggaaacct tcctcgcaac caccgggcgg
     1201 atcttcctgc gcttgctcga gctgcatggc atcgacgcgg acgcgttcgt gcgcgagggc
     1261 ggttatgcgc cggaggcgtt cggcgaccct gaagcgcggc tgccgagcag cgccgtggac
     1321 gtcgcggcgc gagcggcggc tgcgcgcatc gccgatccgg cattcgcgct gcgcgccgcc
     1381 cgctgctggc atccgtcgga cctgggggcg ctcggctacg cctggctcgc aagctcgacc
     1441 ctgcgcaccg cgctgcagcg cctggagcgc tacggccgca tcctcggcga gaaggcgcgc
     1501 ttccggctcg aatccggcga ccgcggcctg cggctcatct tcgagcaccg gcgcaaggac
     1561 gcccgggtgg ccgcgctcgg cacggacttt gcgctgtcgc tcgtgctgga catgtgccgc
     1621 atgaacttcg gcgcctcgct gcggccgctg gaagccacgt tgtgccggcc atgtccggcc
     1681 gacgcgacgc cctggcgggt ctttttcggc ggtgacgtgc gtttcggcgc ggcgcgggat
     1741 tcgatgctgc tgtcacgccg cgacgccgac gcggcgttgc cggtcgccaa tcgccagatt
     1801 gcgggcgccc tcgacgcgat cctggcgcag cagctcgcca ggctggaccg cggcaatatc
     1861 gcggcgcgct gcaaggccga ggtgctcgag cgcctggcct cgggcgaatc gcccgaggcg
     1921 gacatcgcga aatccctgca catgagccgg cggaccctgc agcgcaagct cgccgaagcc
     1981 gatctcactt accagaagct ggtggacgac acgcggcgcg acctggcact gcgctacatc
     2041 gaggatgcgc gcaggtcgat caccgaaatc accttcctgc tcggcttttc ggggcagagc
     2101 gcgtttacgc gcgcctttcg gcgctgggca ggcgtatctc ccacggagta tcgctcgggt
     2161 cggcgcgcgc aggcctaggg cgcgattggc gcgcgcgggc aatcgattgg cgcgggtggt
     2221 gaagcgggct gacgctcgct ccgcgagact cggagcatgt cgacactccg gaaatcccgc
     2281 ggcgcacgat gagggacctg gccaagctcg tgctgctcgg ctgcagcctg tttgcggccg
     2341 cctttgccct ctggcgactg gccctggtgg tcgcgctcgt gatgcgcgag tttcgcggcg
     2401 accgtccggg tgctgaggcg ccggactcga gcgagcaacg cacgtcgtcg acctcaagat
     2461 cgcggtagcg gcccggcgta tcagcccagt catctggcga cgctttgcgg cttagactat
     2521 gcgcatgttc gcgaaagcgg agaccgtgcg ggcctgggtg cgggaagcgc agcgcgtcgt
     2581 ggtgctgacg ggcgccggca tctccactga ctcgggcatc ccggatttcc gcggcccgca
     2641 gggcgtatgg acgcgcaacc cgatggcgga gaagctctcc aacatccact attacatggc
     2701 cgatgcggag gtgcgcaagg cggcctggca ggcgcgcatg gaccacacgg cctggagcgc
     2761 gcagcccaac gccggccatc gcgccatcgc ggcgctggaa aagagcggca agctgcacgc
     2821 gctcatcacg cagaacatcg acgagctgca ccagcaggcg ggcaattcgc cggagaaggt
     2881 gatcga
//
LOCUS       DATDMP010000570         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_978944, whole genome shotgun sequence.
ACCESSION   DATDMP010000570 DATDMP010000000
VERSION     DATDMP010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978944"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VI195_10035"
     CDS             complement(<1..949)
                     /locus_tag="VI195_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY6824769.1"
                     /translation="MTRTLLAPLLAAALAALAQAREPLQAIDDCVARLDSELDVGYAH
                     IAARCPELTPALSASKFAPWLPADWQRPDNELSAAGLSELRAQLARASGAPAHAHPAP
                     RSEQVAAVLAAVARSADDAGRSWWQRLKDWLRGLITTHPQADDAWLRRWLADFKLSNA
                     VTELIGWTALAGVVALAAGIVINELRIAGVLRRRANRARPRPADGERLEACTGLADIE
                     RAAPEEQPALLLELIASRLAAQQRLPSPRALTARELERQARLPGESARAGLAELIGVC
                     ERVRFSARQVSAASRAAALQSGRMLLAALDGAPAAAVVSD"
     gene            complement(954..1799)
                     /locus_tag="VI195_10040"
     CDS             complement(954..1799)
                     /locus_tag="VI195_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824770.1"
                     /translation="MSTVPYPPQRQLSIGEVLDLSFRIYRATLVRCLLFATCGVIAGE
                     LAGIYALIKGGSLPRATGGMAAMLAQLRDPTFVTLYIVGLVLTLLFYAAVLLRERAII
                     TGGSGDAELAVALRRLPALIGLLVLVALGFCMSFVPAAVTGGALRALLISVAAIALCY
                     AVVAISCAQTILLIDEEGPASSLARSWRLTSGSFWRLSLIYSVALIILLALYAVIGAV
                     AGFLAAAFAHGDVAMVTAFAQVIGIILGALATPFYAALALAVLADLKVRKEGTDLAQR
                     ISATA"
     gene            1839..>2260
                     /locus_tag="VI195_10045"
     CDS             1839..>2260
                     /locus_tag="VI195_10045"
                     /inference="COORDINATES: protein motif:HMM:NF037644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824771.1"
                     /translation="MTVGTNLGTVLASGAQRLRTAGNAALAANASLEAGLLLAYALRT
                     TRAKLMSHAERAVEPAAERRYRALIERRARGEPLAYLTGTREFWSLPLTITAAVLVPR
                     PETELLVERALALGSAPQTRVADLGTGSGAVALALASER"
BASE COUNT          368 a          819 c          778 g          295 t
ORIGIN      
        1 cgtccgatac caccgccgcg gccggcgcgc catcgagcgc cgccagcagc atgcggccgc
       61 tctgcaatgc agcggcacgg ctcgccgcgc tcacctgccg ggcggagaag cgcacgcgct
      121 cgcacacgcc gatcagctcc gcgagccccg cgcgcgcgga ctcccccggc aggcgcgcct
      181 gccgctcgag ctcccgagcg gtcagcgcac gcggggaggg caggcgttgc tgagcggcaa
      241 gccgcgaggc gatgagctcg agcagcagcg ccggctgctc ctcgggggcg gcgcgctcga
      301 tgtctgcgag ccccgtgcaa gcctcgagac gctcaccatc cgcgggtcgc gggcgcgcgc
      361 gatttgcgcg gcgacgcagt actccggcga tacgcagctc gttgatgacg atgccggcgg
      421 cgagcgccac tacgccggcg agcgccgtcc agccgatcag ctccgtcacg gcattcgaga
      481 gtttgaagtc cgcgagccag cggcgcagcc acgcatcatc cgcctgcggg tgcgtcgtga
      541 tgagtccgcg cagccagtcc ttcaggcgct gccaccagct gcgcccggcg tcatccgcgc
      601 tgcgcgcgac cgcggcgagc acagccgcaa cctgctcgct gcgcggtgcg ggatgagcgt
      661 gcgccggcgc gccgctcgcg cgagccagct gcgcgcgcag ctcgctcagg ccggcggccg
      721 acagctcgtt gtcgggccgc tgccagtccg cgggcagcca gggtgcgaac ttgctcgcgc
      781 tgagtgcggg tgtcagctcg gggcagcgcg cagcgatgtg cgcatagccg acgtcgagct
      841 cgctgtcgag ccgggccacg caatcatcga tcgcctgcag cggctcgcgc gcttgcgcta
      901 gagcggcgag cgcggctgcc agcagcggcg cgagcagcgt gcgcgtcacg ccgtcaggcc
      961 gtcgccgaga tgcgttgcgc gagatcggtg ccctctttgc ggaccttgag atcggcgagt
     1021 accgccaatg ccagtgcggc atagaaagga gtcgccagcg cgccgaggat gatgccgatg
     1081 acctgcgcga atgcggtcac catcgccaca tcgccgtgcg cgaacgccgc ggccagaaat
     1141 ccggctaccg caccgatgac cgcatagagc gcgagcagga tgatgagcgc cacggagtag
     1201 atgagactca gccgccagaa gctgccgctg gtcaggcgcc agctgcgcgc gaggctgctc
     1261 gccgggcctt cctcatcgat gagcaggatg gtctgcgcgc aggatatcgc caccacggcg
     1321 tagcagagcg ctatcgccgc gacgctgatc aacaacgcgc gcagtgcgcc accggtaacc
     1381 gccgccggta cgaaggacat gcaaaatccc agcgccacca gcaccagcag accgatgagg
     1441 gccggaaggc gccgcaaggc aacggccagt tcggcatcgc cgctgccacc ggtgatgatg
     1501 gcgcgttccc gcaacagcac cgcggcgtag aacagcagcg tgagcaccag acccacgatg
     1561 tacagcgtca cgaaggtcgg atcacgcagc tgtgcgagca tcgccgccat gccgccggtc
     1621 gcccgcggca gtgagccgcc cttgatcagc gcgtagatgc ccgccagctc tccggcgatg
     1681 acgccgcagg ttgcaaacaa caggcagcgc acgagcgtcg cccggtagat gcggaagctc
     1741 agatccagca cctccccgat cgacagctgt cgctggggag ggtacggtac ggtgctcatt
     1801 caactatcat aacgccgcac cgcctgagta gcctacggat gacggtcggc accaacctcg
     1861 gcacggttct tgcgagcgga gcacagcggc tgcgtaccgc tggcaacgct gccctcgcgg
     1921 ccaatgcctc cctggaggcc ggcctgctgc tcgcctacgc gttgcgcacc acacgtgcca
     1981 agctgatgtc gcacgcggag cgcgcggtcg agcccgctgc cgagcggcgc taccgtgcac
     2041 tcatagagcg gcgcgcccgc ggggagccgc tcgcctacct caccggcaca cgcgagttct
     2101 ggtccttgcc gctgacaata acagcggcgg tgctcgtgcc gcgtccggag accgagctgc
     2161 tcgtggagcg cgcgctcgcg ctcggcagtg cgccgcagac tcgcgtcgcc gatctcggca
     2221 ccggctcggg cgcggttgcc ctcgccctcg ccagcgaacg
//
LOCUS       DATDMP010000571         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1313255, whole genome shotgun sequence.
ACCESSION   DATDMP010000571 DATDMP010000000
VERSION     DATDMP010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313255"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VI195_10050"
     CDS             complement(<1..722)
                     /locus_tag="VI195_10050"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6824772.1"
                     /translation="MLLAAYCAAAPAAGLPGREGIVVTPDQEGLAGQADAASQGTVED
                     DQLENRPLLRPAEILEVIPGVIVTQHSGSGKANQYFLRGFNLDHGTDFAVHVDGMPIN
                     LPSHGHGQGYADLNFLIPELVEHIHYRKGPYYADEGDFSAAGAAHVHLKRSLSGNLAE
                     LTTGSNGYRRGLFGAQRALEGGNLLGMLELDQADGPWQNPEQLRKLNGVLRYSRGDDE
                     NGFSVTGMAYANRWNATDQIPLR"
     gene            complement(848..1432)
                     /locus_tag="VI195_10055"
     CDS             complement(848..1432)
                     /locus_tag="VI195_10055"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY6824773.1"
                     /translation="MGAIFQSLNRTLVAGVVILVAVMLIAGTFGRMGDMAWWTFFFRY
                     LHILSGVMWIGLLWYFNFVQTPSMPKIPDEQKPAVSKVIAPAALFWFRWGAMSTIVTG
                     AILALMNNYLVQALSLQKPFTAIGLGMWFGIIMWFNVWFIIWPNQKKVLGIVTVGPEE
                     KAKAARMAGMTSRFNTMFSIPMLFFMAAQSHGGL"
     gene            complement(1488..1904)
                     /locus_tag="VI195_10060"
     CDS             complement(1488..1904)
                     /locus_tag="VI195_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824774.1"
                     /translation="MGMTPPVESEAQEAYARWLDWGTRIALAVSVVALAVYLSGALAP
                     WVPLDQLPTLWSLPVSQFIERAHAPTGWAWLRLVGYSDYLNYVGIALFALVSVPCLLR
                     AIPCFLRRGERVQAGLALAQVVVLVAAASGWFAGGR"
     gene            complement(1895..>2206)
                     /locus_tag="VI195_10065"
     CDS             complement(1895..>2206)
                     /locus_tag="VI195_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY6824775.1"
                     /translation="GLGAGFANVPALNLLMGAPLKVAVASSGLIISIINSSAAWVYIN
                     RGAVLPLLAVPALLGISLGSRIGARLLRVTPAVVVRRIVIVVMLIAGVRALLRGLGVW
                     A"
BASE COUNT          432 a          712 c          726 g          336 t
ORIGIN      
        1 cgcagcggaa tctggtccgt agcgttccag cggttggcat acgccatgcc ggtcacgctg
       61 aagccgttct catcgtcgcc gcgcgaatag cgcagtacgc cgttcagctt gcgcagctgc
      121 tccgggttct gccagggccc gtccgcttgg tcgagttcca gcatgccgag cagattcccg
      181 ccctcgagcg cccgctgcgc gccgaagagc cctcgtcgat agccgttgga cccggtcgtg
      241 agctccgcga gattgccgct gagcgagcgc ttcaggtgca catgcgcggc cccggccgcg
      301 gaaaagtcgc cttcgtcggc gtagtacggg cccttgcggt aatggatgtg ctccacgagc
      361 tcgggtatga ggaaattgag atccgcatag ccttgtccgt ggccatggct cggcagattg
      421 atcggcatcc cgtcgacatg gaccgcgaaa tcggtgccgt gatcgagatt gaacccgcgc
      481 aggaaatact ggttcgcctt gccgctgccg ctatgctggg tcacgatcac gcccggaatc
      541 acctcgagga tttcggccgg tcgcaggagc gggcgattct ccagctggtc gtcctccacg
      601 gtgccctggc tcgcggcatc ggcctgtccg gcaagaccct cctggtccgg ggtcactacg
      661 atgccttctc gccccgggag acccgccgcc ggcgctgccg cgcagtaagc cgcgagcagc
      721 aaagaagcga gctccctacg cgggcacatt gaggcgcagg cctaccagca gcgatgatgc
      781 gtttctcacg ctcaaaaaag atcggggccc gcgaagggcc ccgacgtccg gtgcagatcc
      841 tgctggtcta caggccgccg tgcgactgcg cagccatgaa aaacagcatc ggaatcgaga
      901 acatggtgtt gaagcgcgag gtcatgccgg ccatgcgggc ggccttcgcc ttctcctccg
      961 ggccgaccgt cacgatgccg agcaccttct tctggttggg ccagatgatg aaccagacgt
     1021 tgaaccacat gatgatgccg aaccacatgc ccaggccgat ggcggtgaag ggcttctgca
     1081 ggctgagcgc ctgcacgaga tagttgttca tcagcgcgag gatcgcgccg gtcacgatcg
     1141 tggacatggc gccccagcgg aaccagaaga gcgcggccgg ggcgatgacc ttgctaacgg
     1201 ccggcttctg ctcgtccggg atcttcggca tggaaggcgt ctgcacgaag ttgaagtacc
     1261 agagcaggcc gatccacatc actccggaga ggatgtgcag gtagcggaaa aagaaggtcc
     1321 accacgccat gtcgcccatc cggccgaacg tgcccgcgat gagcatgact gcgacgagaa
     1381 tgacgacgcc cgcgacgaga gtgcggttca gtgactggaa gatcgcgccc atagtgttca
     1441 cctcttggtt tgggaatggg gttgaacgcg aaaaaagtct agcacggtca tcggcccccg
     1501 gcgaaccagc cggaggccgc cgcgacgagg acgacgacct gggctagcgc aaggccggcc
     1561 tgcactcgct cgccacgcct caggaagcac gggatggcgc gcagcaggca cggaaccgag
     1621 acgagcgcaa agagcgcgat gcccacgtag ttcagataat ccgaataacc gacgaggcgc
     1681 agccaggccc agccggtcgg ggcatgggcc ctttcgatga attgcgacac cggcaatgac
     1741 cagagcgtgg gaagctggtc gagcggcacc cagggcgcca gcgcgccgga aagataaacc
     1801 gcgagcgcga cgacggaaac cgccagcgcg atccgcgtcc cccagtcgag ccagcgggca
     1861 tacgcctcct gggcttcgga ctcgaccggg ggcgtcatgc ccatacgcct aatccgcgca
     1921 gcaaggcgcg cacgcccgcg atcagcatta ccacgatgac gatgcgtcga accacgacgg
     1981 ccggcgtcac gcgcagcagg cgcgcgccaa tgcgcgagcc aagcgagata ccgagcagcg
     2041 ccggcaccgc gaggagcggc agcaccgcgc cgcggttgat gtacacccac gctgccgagc
     2101 tgttgatgat ggaaatgatg aggccgctgc ttgccaccgc gaccttgagc ggcgcaccca
     2161 tgaggaggtt gagcgcgggg acgtttgcaa acccggcgcc gaggcc
//
LOCUS       DATDMP010000572         2631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1979711, whole genome shotgun sequence.
ACCESSION   DATDMP010000572 DATDMP010000000
VERSION     DATDMP010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979711"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VI195_10070"
     CDS             <1..154
                     /locus_tag="VI195_10070"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY6824776.1"
                     /translation="TVGVTEYDALPVNARRYLERLEALVGVPIDMISTGPDREQTIVL
                     RHPFAG"
     gene            232..1989
                     /locus_tag="VI195_10075"
     CDS             232..1989
                     /locus_tag="VI195_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824777.1"
                     /translation="MLASGCWRVYSNTWDEPEHLAAGIELLDRGKYEYDTEHPPLARL
                     FLAIGPYLAGAHSYGTPPPEGTPEGLHILYAKGAYWRDLTLARLGMLPFLALLLVATW
                     LWARRLLPSEGAALLAVLLLVSVPPVLGHAALASLDVAAAGTILLALYALQCWIASAR
                     LRDAARFGLSAGLAVVTKFSAVPFIALALIALALTHFAVTQAGRGRLDAPSFWRLRVG
                     GLALAGLVTLVPVWIVYGPRVADPAGVAFRFNWAVSYLLQQHGAADGLGVLLSHLWLP
                     RELKDLVNGIVAVEAHNDSGHLSYLLGEVRLTGWWYFYLVALAVKTPLPLLIAGPIGM
                     GWLAVSGWRRRDSWALAPLVIVVAILGFASAVSRINIGIRHVLILYPFFALGGAFVTV
                     KAWRAARNLRPPAAAAPTAAERTAEPPPAATAAAAHRDLPAIVHHLVNHWFGHLQNPR
                     RTLTAAAIAAALAWQLSPLWRAYPDYLPYFNETVGAHPERVLVDSDLDWGQDLHRLEV
                     RAAQLRIGHLNLAYRGTADLRREPLPPLHILPPRERAIGWVAVSELARTRNRADYAWL
                     DGYRPIERIGKTIDLYYVP"
     gene            complement(1977..>2631)
                     /locus_tag="VI195_10080"
     CDS             complement(1977..>2631)
                     /locus_tag="VI195_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6824778.1"
                     /translation="LKAHIEALLKRCGFASKGVFVVDNSRRSSHGNAYFTGIGRHKRI
                     VFFDTLLEHLGHPEVEAVLAHELGHFRLKHVRKRLLLSMASMFLGLALLGWLTGQPGF
                     YHALGVPVPSTHAALLLFVLVVPVFTFFVTPLASLWSRRHEFEADAFASQHASAGELA
                     TALVKLHRDNASTLTPDPVYAAFYYSHPPPLTRITRLRAAAAAAARAASLGLSPQGT"
BASE COUNT          378 a          903 c          903 g          447 t
ORIGIN      
        1 cacggtcggc gtcactgaat acgacgcgct gccggtgaac gcacggcgct acctcgagcg
       61 gctcgaggcg ctggtcggcg tccccatcga tatgatctca acgggacccg accgcgagca
      121 gaccatcgtg ctgcggcacc cgttcgcagg gtgagctcga ccgcacccgc caccacttct
      181 gcgccacgga cggcgcgcaa ggctctggcg ctcctgattc tcttggcgtg cgtgctcgcg
      241 agcgggtgct ggcgggtcta ctccaacacc tgggacgagc ccgagcatct cgccgccggc
      301 atcgagctgc tcgatcgcgg caagtacgag tacgacaccg agcatccacc gctcgcgcgg
      361 ctgttcctcg ccataggccc gtatctcgcc ggggcccact cctacggcac accgcccccg
      421 gagggaactc cagaggggct ccacatcctc tatgcgaagg gcgcgtactg gcgcgatctg
      481 accctcgcac ggctcggcat gctgcccttc ctggcgctgc tcctcgtggc gacctggctc
      541 tgggcacggc ggctcctgcc ctcggaaggg gccgcgctgc ttgccgtgct gctcctggtg
      601 tcggtgccgc ccgtgctcgg gcacgcggcg ctggcgagtc tcgatgtcgc ggcggcgggg
      661 acgatactgc tcgcgctcta cgcgctgcag tgttggatcg cgagcgcgcg cttgcgggat
      721 gcggcgcgct tcgggctcag cgcgggtctc gccgtggtca cgaagttctc cgcggtaccg
      781 ttcatcgcgc tggcgctgat cgcactggcg ttgactcact tcgcggtcac gcaagccggt
      841 cgcggacggc tcgacgcgcc ctcattctgg cgtttgcgcg tcgggggtct cgcacttgcg
      901 gggctggtga cgctcgtccc ggtgtggatc gtttacggtc cgcgcgtggc ggatccggcc
      961 ggcgttgcgt tccgcttcaa ctgggcggtg tcctatcttc tccaacagca cggcgcggcg
     1021 gatggcctcg gcgtgctgct ctcgcacctg tggctgccgc gcgagctgaa agacctggtg
     1081 aacggcatcg tcgccgtcga ggcgcacaat gacagcgggc acctctcgta cctgctgggt
     1141 gaggtgcggc tcaccggctg gtggtacttc tacctcgtgg cgctggccgt gaagacgccc
     1201 cttccgcttc tcatcgcagg tcccatcggt atgggttggc tcgcggtgtc cggctggcgg
     1261 cgccgcgatt cctgggcgct cgcgccgctc gtgatcgtgg tggcgatcct cggcttcgcg
     1321 agcgcggtga gccgcatcaa catcggcatt cggcacgttc tgattctcta tccgttcttc
     1381 gcgctcggtg gggccttcgt gacggtgaag gcctggcggg cagcgcgaaa cctgcggccc
     1441 ccggcggctg ctgcgccgac ggccgctgag cggaccgctg agccgccgcc ggcggcgacg
     1501 gcagccgcgg cgcatagaga ccttcccgcg atagttcacc atttggtaaa ccattggttc
     1561 ggacaccttc aaaacccccg ccgcacgctc accgccgccg cgatcgccgc agcgctcgcc
     1621 tggcaattga gcccgctgtg gcgcgcgtat cccgactacc ttccgtactt caatgagacg
     1681 gtcggcgcgc acccggagcg ggtgctggtc gattcagatc tcgattgggg gcaggacctg
     1741 catcggctgg aggtgcgcgc cgcgcagctg cgcatcgggc atctcaacct cgcctaccgc
     1801 ggtaccgcgg acttgcggcg cgagccgctg ccgccgctgc acatcctgcc gccaagggaa
     1861 cgtgccatcg gctgggttgc ggtatccgag ctcgcgcgca cgcgcaatcg cgccgattac
     1921 gcctggctcg atggctatcg cccgatcgag cgcatcggca agaccatcga cctctattac
     1981 gtgccctgag gtgaaaggcc gagcgaggcc gctcgggctg ccgccgcagc ggcggcccgc
     2041 agccgcgtga tgcgcgtgag gggcgggggg tgcgagtagt agaacgccgc gtacaccgga
     2101 tccggcgtga gcgtgctcgc attgtcgcga tggagcttga cgagcgccgt ggccagctcc
     2161 ccggcactcg catgctgtga ggcgaaggca tccgcctcga actcgtgccg ccgcgaccag
     2221 agcgaggcga gcggcgtcac gaagaaagtg aacaccggca ccacgagcac gaacaagagg
     2281 agtgcggcgt gggtcgaggg caccgggaca ccgagcgcgt ggtagaagcc cggctgaccg
     2341 gtgagccagc ccaggagcgc gagccccaga aacatgctcg ccatcgagag cagcagccgc
     2401 ttgcgcacgt gcttcaggcg aaagtgcccg agctcgtgtg ccagcacggc ctcgacttcc
     2461 gggtgcccga ggtgctcgag cagcgtatcg aagaaaacga tgcgcttgtg ccgcccgatg
     2521 ccggtgaagt aggcgttgcc gtggctcgag cggcgcgaat tatccaccac gaacactccc
     2581 ttcgaggcga agccgcagcg cttgagcagc gcctcgatgt gcgccttgag c
//
LOCUS       DATDMP010000573         4528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_979271, whole genome shotgun sequence.
ACCESSION   DATDMP010000573 DATDMP010000000
VERSION     DATDMP010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979271"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4033)
                     /gene="acnA"
                     /locus_tag="VI195_10085"
     CDS             complement(<1..4033)
                     /gene="acnA"
                     /locus_tag="VI195_10085"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY6824779.1"
                     /translation="MNNSFRARSTLKVGDQTYEIWSLAALPPERLARLPYSLKVLLEN
                     LLRYEDGVNVTRADIEALLEWDPAATPAHEIAFTPARVILQDFTGVPCIVDLAAMRDA
                     IVRLGGSAGRVNPLNPAELVIDHSVQVDEYGSPHALAANTAIEFARNRERYAFLRWGQ
                     TAFRNLSVVPPSTGIVHQVNLEYLARVIFDAPNNGTRRAYPDTLVGTDSHTTMINGLG
                     VLGWGVGGIEAEAAMLGQAVTMLIPQVIGFRLEGRLQPGATATDLVLTVTEMLRKKGV
                     VDKFVEFFGDGLANLPLADRATIANMAPEYGSTCGIFPIDAETVRYLELSGRPRERLE
                     LVSAYARAQGMWRNEGALPASYTDVLELDLGTVEPSLAGPRRPQDRVALKNAKQVYEL
                     NAKKAAEERSARGTGAHGVATAKLNGGSYQLKDGAVLIAAITSCTNTSNPSVMLGAGL
                     LARKARARGLKSKPWVKTSLAPGSRVVTDYFAKAGLLEDLDALGFDLVGYGCTTCLAA
                     GTPVLLANGTSRRIESLPAAGGVRLFGPTADRRLAMASQTEMMVQGERECISIVLQDG
                     RRLVCTEDHKLLCADGRWVPAGRLVPGRDRVLAGLEAPLDEPGADEVGYELTAGGMRF
                     TFANEHERACTLAFARLVGHLISDGSISVTGQGRMHLGQALDREAALDDIELLTGKRP
                     VAKRYDPRKWTLVLPVDLTAAITALAGVRRGRRIHQPPALPGFVLDDRCPVSVVREFL
                     AALFGADGHAPMLARQGESEASAILRPPAYVQSAKTEHVEALREVMQQIVQLLVRCGV
                     KARGAQIYTYPTRRTRSRYPAARDGAERIEFRLALPEGLSFVERVGFRYCVNKSLRAS
                     AAAVYWRTIDTINRQRLWMADRIEALRENYDFSFQQTRRLAPAVLMTYETAPFPQHSL
                     LEGHDRFDRLPGTGNRSGCKPQHREQCDFPSPAQLLKEIGARDWFARLQPQDGAAVFA
                     PRYCADKHAVTLPTLSLQVRAVRPDGPRRVFDLAVDDIHAFIAGTVSVHNCIGNSGPL
                     KPEISAAVKEGDLTAASVLSGNRNFEGRVHPETRMNFLASPPLVVAYALAGTLDIDLT
                     REPLGTDKDGKAVHLADIWPSDAEIQELAARSIDSQMFRQSYASVFKGDDNWANISVP
                     TGQLYTWDEKSTYVKNPPYFAGMTMQLKPLADVRGARVLAVLGDSVTTDHISPAGNIS
                     RSSPAARYLIEQGVEPKDFNSYGARRGNHEVMMRGTFANIRLRNLMVPGVEGGVTLHL
                     PDGERGSIYDIAMRYQSEGTPLVVLAGREYGTGSSRDWAAKGTMLLGVKAVIAESFER
                     IHRSNLIGMGVAPLQFLPGQNAAS"
     gene            complement(4112..>4528)
                     /locus_tag="VI195_10090"
     CDS             complement(4112..>4528)
                     /locus_tag="VI195_10090"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEY6824780.1"
                     /translation="LAFYTLGQRAGLHIGGRCGRAPAPWYIAAKDAGRNALIVVQGHD
                     HPLLASCGLVTGHWHWLAPPRREPFGAAVKVRYRQSDQAARLEPRPDGTMHIAFAEPQ
                     RAVTPGQYAVAYDGERCLGGAVIESLVRTALLSAAA"
BASE COUNT          754 a         1489 c         1514 g          771 t
ORIGIN      
        1 gagaggcggc gttctgcccc ggcaggaact gcagcggcgc gacacccatt ccaatcagat
       61 tggagcggtg aatgcgctcg aagctctcgg cgatgaccgc cttgaccccg agcagcatgg
      121 tgcccttggc cgcccagtcg cgtgaggagc cggtgccgta ctcacgtcct gcgagcacga
      181 cgagtggggt accttcgctc tggtagcgca tcgcgatgtc gtaaatcgag ccacgctcgc
      241 catcgggaag atgcagggtc actccgccct cgacacccgg caccatcagg ttgcgtaggc
      301 ggatgttggc gaaggtgccg cgcatcatga cctcgtgatt gccgcggcgg gccccgtagg
      361 aattgaagtc tttcggctcc acgccctgct cgatgagata gcgtgccgcg gggctgctcc
      421 tggagatgtt gcccgccggg gagatgtgat cggtggtcac cgagtcgccg agtaccgcga
      481 gcacccgcgc accgcgcaca tccgcgagcg gcttgagctg catggtcatg cccgcgaagt
      541 agggcggatt cttcacatag gtggacttct cgtcccaggt atagagctgt ccggtgggca
      601 cgctgatgtt ggcccaattg tcgtcgccct tgaagacgct cgcgtagctc tgccggaaca
      661 tctgcgagtc gatcgagcgt gcggcgagct cctggatctc cgcatcactc ggccagatgt
      721 cggcgaggtg gaccgctttg ccatccttat cggtcccgag cggctcgcgt gtcagatcga
      781 tgtcgagtgt gccggcgagt gcatacgcca ccacgagcgg cggtgaagcg agaaaattca
      841 tgcgcgtctc ggggtgcacg cggccctcga agttgcggtt gccggagagc accgatgccg
      901 ccgtcagatc gccctccttg acggcggcgg agatttcggg cttgagcgga ccggaatttc
      961 cgatgcagtt gtgaacgctg acggtgccgg cgatgaaggc gtggatgtcg tcgacggcca
     1021 ggtcgaacac gcggcgaggt ccgtcggggc gtacggcgcg gacttgcagc gagagagtgg
     1081 gcagagtcac agcgtgcttg tccgcgcaat accgcggtgc gaacaccgcg gcaccgtcct
     1141 gaggctgcag ccgcgcgaac cagtcgcgcg caccgatctc cttcaagagc tgcgcgggcg
     1201 aggggaaatc gcactgctcg cgatgctggg gcttgcaccc cgaacggttt ccggtcccgg
     1261 gcaagcggtc gaagcggtcg tgaccctcga gaagcgagtg ttgtgggaac ggcgcggtct
     1321 cgtaagtcat cagcaccgcg ggcgccaggc gccgcgtctg ctgaaagctg aagtcgtagt
     1381 tctcgcgcaa cgcctcgatg cgatctgcca tccacaggcg ctggcggttg atggtgtcga
     1441 tggtgcgcca gtacacggcc gcagcgctgg cacgcaggga tttattgacg cagtaacgga
     1501 agccgacgcg ctcgacgaag gaaaggcctt ccggaagtgc gaggcggaac tcgatgcgtt
     1561 ccgccccatc gcgggcggcc ggatacctcg aacgtgtgcg cctcgtcgga taggtgtaga
     1621 tctgcgcgcc gcgagctttg actccacagc gcaccagcag ctgcacgatc tgctgcatga
     1681 cttcccgcag cgcctccacg tgctcggtct ttgcgctttg gacataggcc ggtggcctca
     1741 ggatcgcact cgcttcgctc tccccctgcc gggcaagcat cggtgcatgc ccgtccgcac
     1801 cgaagagtgc cgccaggaac tcgcgtacca ctgacaccgg acatctatca tcgagtacga
     1861 agccgggcag cgcgggcggt tgatggatgc gccgtccgcg acgcacgcca gcgagtgcgg
     1921 tgattgcggc ggtcagatcg accggaagta ccagggtcca cttgcgagga tcgtaacgtt
     1981 tcgcgaccgg acgcttgccg gtcagcagct cgatgtcatc gagcgccgcc tcgcggtcga
     2041 gagcctggcc gagatgcatg cgtccctggc ccgttacgct gatcgaaccg tcgctgatca
     2101 gatgcccgac gagtcgtgcg aaggccaaag tacacgcacg ctcatgctcg ttggcgaagg
     2161 tgaaacgcat cccgccagcg gtcagctcgt aacccacctc atcggcgccg ggttcatcga
     2221 gcggagcctc gagcccggcg agaacgcgat cgcgccccgg cacgagcctg cccgcgggaa
     2281 cccaacggcc atcggcacac aggagcttgt ggtcctcagt gcatacgagg cggcgaccgt
     2341 cctgcagcac tatggagatg cactcgcgct cgccctgcac catcatctcg gtctggctcg
     2401 ccatagcgag ccgccggtct gcggtgggcc caaacaggcg cacgccgcct gctgccggta
     2461 agctttcgat ccggcggctc gtcccattgg cgagcagcac cggggtcccg gccgcgaggc
     2521 acgtggtgca accgtagccc accagatcga agccgagcgc atcgagatcc tcgagcagtc
     2581 cggctttggc gaagtagtcg gtaaccaccc gtgagccggg cgcaaggctc gtcttcaccc
     2641 acggcttcga cttcagtccg cgcgcgcgcg ccttgcgcgc cagcaggcct gcgccgagca
     2701 tcaccgaggg gttggaagtg ttggtgcagc tggtaatcgc ggcgatcagc accgcaccgt
     2761 ccttgagctg gtagctgcca ccgttgagtt tcgccgtcgc caccccgtgc gcgcccgtac
     2821 cgcgcgcgct gcgctcctcg gccgccttct tcgcgttcag ctcgtacacc tgcttcgcat
     2881 tcttgagcgc cacccggtcc tgcgggcgcc gcgggcccgc caggctcggc tcgacggtcc
     2941 cgagatcgag ctccaggaca tcggtgtagc tcgcgggcag cgcaccttcg ttgcgccaca
     3001 taccctgtgc gcgcgcgtag gcactcacca gctcgagccg ctcgcgcggc cggccggaga
     3061 gctccagata tcgtaccgtc tcggcatcga tgggaaagat gccgcaggtg ctgccgtact
     3121 cgggcgccat gttcgcgatg gtggcgcgat cggcgagtgg cagattggcg agcccatcgc
     3181 cgaagaactc gacgaacttg tcgaccacgc ccttcttacg gagcatctcc gtcacggtaa
     3241 gcaccaggtc ggtagccgtc gccccgggct gcagccgccc ctcgaggcgg aaaccgatca
     3301 cctgcggaat gagcatggtg accgcctggc cgagcatcgc cgcctcggcc tcgatgccac
     3361 cgacgcccca gccgaggaca ccgaggccgt tgatcatggt ggtatgcgag tcggtgccca
     3421 ccagggtatc gggataggca cggcgcgtgc cgttgttggg agcgtcgaag atcacccgcg
     3481 cgagatattc gaggttcacc tgatgaacga tgccggtgct cggtggcact accgagagat
     3541 tgcggaacgc agtctgtccc cagcgcagga acgcataacg ctcgcgatta cgtgcgaact
     3601 cgatcgcggt gttggccgcg agtgcatgcg ggctgccgta ttcatccacc tgcaccgaat
     3661 gatcgattac cagctctgcg gggttgagcg gattgacgcg accggcgctg ccgccaaggc
     3721 gcacgatcgc gtcgcgcatc gcggcgagat cgacgatgca gggtacgccg gtgaagtcct
     3781 gcagaatgac ccgcgcaggc gtgaatgcga tctcgtgcgc gggcgttgcg gccgggtccc
     3841 actcgagcag cgcttcgatg tcggcgcgcg tcacgttgac gccgtcctcg tagcgcaaca
     3901 gattctccag gagcaccttg agcgagtagg gcaagcgcgc gagcctctcc ggtggcaacg
     3961 cggccaggct ccagatctcg taggtctgat cgccaacctt gagggtcgag cgtgcccgga
     4021 agctgttgtt catgggtgtc tccgcgttgt gcacgcgatt atatcgggcg cacccgctgc
     4081 ctgccggctc ggcgacgccg cggtccgctt ttcaggctgc cgcggacagt agcgcggtac
     4141 gtaccagcga ctcgatcacc gcgccgccga gacagcgctc accgtcatac gcaaccgcgt
     4201 actgacctgg cgtgaccgcc cgctgtggct ccgcgaacgc gatgtgcatg gtgccatcgg
     4261 gccgcggctc gagccgtgcc gcctggtcgc tctggcggta gcgcaccttc acggcagcgc
     4321 cgaaaggctc gcgccgtggc ggcgcgagcc agtgccagtg acccgtcacg agcccacagc
     4381 tcgcgagcag cggatgatcg tggccctgca ccacgatcag cgcgttgcgt cccgcatcct
     4441 tggccgctat gtaccagggt gccggcgcgc gtccgcaacg gccgccgatg tgcagacccg
     4501 cgcgctgacc gagggtgtag aaggcgag
//
LOCUS       DATDMP010000574         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1534449, whole genome shotgun sequence.
ACCESSION   DATDMP010000574 DATDMP010000000
VERSION     DATDMP010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534449"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VI195_10095"
     CDS             <1..355
                     /locus_tag="VI195_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY6824781.1"
                     /translation="NRGITVLMKSVDAAYAAAMARLPTPKLTRTLMQAVERQQPPRAG
                     MGRPKLRYAHQGGHNPPLIVIHGSALASIPDSYRRYLEHFFAETFQLRGTPLRIQFKT
                     GRNPYAAASPRRQNR"
     gene            450..698
                     /gene="hfq"
                     /locus_tag="VI195_10100"
     CDS             450..698
                     /gene="hfq"
                     /locus_tag="VI195_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY6824782.1"
                     /translation="MSNKGQMLQDPFLNTLRKEHVRVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARAVSMPHHAPSSDSEA"
     gene            747..1877
                     /gene="hflX"
                     /locus_tag="VI195_10105"
     CDS             747..1877
                     /gene="hflX"
                     /locus_tag="VI195_10105"
                     /inference="COORDINATES: protein motif:HMM:NF008280.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY6824783.1"
                     /translation="MFERPGGGDRALLVVLDLGDGNPGDRLAELKELALSAGAEVVGV
                     VTGRRKRPDAALFAGKGKVEEIAAQRLETEADLVIFDHPLTGAQQRNLERALECRVVD
                     RTSLILDIFALRARSAEGKLQVELAQVKHQMTRLVGGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLGTRERILKERLRHVAQQRATQSRTRRRALVRTVALVGYTNAGKSTLFNRLT
                     GAQAYAANQLFATLDTTLRRLNLPDADDIVVSDTVGFVRELPHDLVAAFRATLSEAAD
                     ADLLLHVIDASHPNREEQIEAVEGVLHEIGADEVPQIRVYNKIDLAGLAPGMERDASG
                     ILHAIRLSALTGEGCAELRAALVERFPGRSEARAHTPAAVQK"
     gene            1960..>2336
                     /locus_tag="VI195_10110"
     CDS             1960..>2336
                     /locus_tag="VI195_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HEY6824784.1"
                     /translation="MSLNDPQWGKRGNSGPPDLDEIWRNVNRKINDLFGRKNSGGTPS
                     EGGGPRFRGMLPLGGAGLLVALVLLVWLASGFYIVDEGRRGVVTRFGKYTETTLPGPR
                     WHLPYPVETVELVDFSQVKTIEVG"
BASE COUNT          435 a          777 c          768 g          356 t
ORIGIN      
        1 aaaccgtggc atcaccgtgc tgatgaaatc ggtggacgcc gcatacgctg cggcgatggc
       61 gcgcctgccg acacccaagc tcacgcgcac gctcatgcag gccgtcgagc gacagcagcc
      121 gccgcgggcg ggcatgggcc ggcccaagct gcgctacgcc caccagggcg ggcacaatcc
      181 cccgctcatc gtgatccatg ggagcgccct ggccagcatc ccggattcgt accggcgata
      241 tctggaacat ttctttgccg agacgttcca actccgcggc acccccctca ggatacaatt
      301 caagaccggt cgcaatccct atgccgctgc atccccaagg cgccagaacc gttagcaaag
      361 cacggcagcc aggactcctg gaagacgcgg gagccgttgc aaggcaagga accgccggag
      421 cgcgctcgac aacaaccgcg gagcaagcga tgagcaataa agggcaaatg ctacaagacc
      481 cgtttctgaa cacgctgcgc aaggaacacg tgcgggtttc gatctacctc gtgaacggca
      541 tcaagctcca ggggcagatc gagtcgttcg accagtatgt tgttctgctc aagaacacgg
      601 tgacgcagat ggtctacaag catgcgatct cgaccgtggt gccggcgcgc gcggtatcga
      661 tgccgcatca cgcgccgagc agcgacagcg aggcctgacc gtcctatcga atcgcacgga
      721 ccggtgctcg ccggtccgca ccgacgatgt ttgagcgccc gggcggcggc gatcgcgcgc
      781 tgctggtcgt gctcgacctg ggcgacggca atcccggcga tcgcctcgcg gagttgaagg
      841 agctcgcgct gtcggcgggc gcggaggtgg tgggcgtcgt cacgggacgg cgcaagcggc
      901 cggatgcggc gctcttcgca ggcaagggca aggtcgagga gattgccgcg cagcgtctcg
      961 agaccgaagc ggatctcgtc attttcgatc acccgctcac cggcgcgcag cagcgcaatc
     1021 tcgagcgtgc cctcgaatgc cgcgtggtcg atcgcacgag cctcatcctg gacatctttg
     1081 cgctgcgcgc gcgcagcgcg gagggcaagc tgcaggtgga gctcgcccag gtcaagcacc
     1141 agatgacgcg cctcgtgggc ggctggaccc acctcgagcg ccagaaaggc ggtatcggtc
     1201 tgcgcggccc gggcgagacg cagctcgaga ccgaccggcg actgctgggc acgcgcgagc
     1261 gcatcctgaa ggagcggttg cgccacgtcg cgcaacaacg tgccacgcaa agccgcacgc
     1321 ggcggcgtgc gctggtgcgc acggtcgcgc tcgtgggata taccaacgcc ggcaagtcga
     1381 cgctgttcaa ccggctcacc ggcgcgcagg cgtatgccgc aaatcagctt ttcgcgaccc
     1441 tcgacacgac gctgcgcagg ctcaacctgc ccgacgccga cgacatcgtc gtatccgata
     1501 cggtaggctt cgtgcgcgag ctgccccatg atctcgtggc cgcgtttcgc gccacgctgt
     1561 ccgaagccgc cgatgcggat ctgctgctgc acgtgatcga tgcctcgcac ccgaatcgcg
     1621 aggagcagat cgaagccgtc gaaggtgtgc tgcacgagat cggtgccgac gaggtgccgc
     1681 aaatccgcgt ctacaacaag atcgatctcg cggggctcgc gcccgggatg gagcgggatg
     1741 cctctggtat actgcatgcg atccgattga gcgcgttgac cggcgaggga tgcgcagagt
     1801 tgcgggctgc tctcgtcgag cgatttccgg ggaggagtga agcccgggcc cacacgccgg
     1861 cggcggtgca aaaataacca gcaacccgcc cggcggcctt ctccgcgccg cctcggcagg
     1921 cgacaattcc cacatgacct ccggtccatc acgacataca tgtcgctgaa cgatccccaa
     1981 tggggaaagc gcggcaacag cggaccgccg gatcttgacg agatctggcg caacgtcaac
     2041 cgcaagatca acgatctctt cggccgcaag aacagcggcg gcaccccctc cgagggtggt
     2101 ggaccgcgct ttcgcggcat gctgcccctg ggcggcgcgg gtctgctcgt cgcgctggtg
     2161 ctgctcgtgt ggctggcgag tggcttctac atcgtcgatg aaggccggcg cggcgtagtg
     2221 acccgcttcg ggaagtacac cgaaacgacc ctgcccggcc cgcgctggca cctgccctac
     2281 cccgtcgaga cggtcgagct cgtcgacttc tcgcaggtga aaacgatcga ggtcgg
//
LOCUS       DATDMP010000575         2751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_535227, whole genome shotgun sequence.
ACCESSION   DATDMP010000575 DATDMP010000000
VERSION     DATDMP010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535227"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VI195_10115"
     CDS             complement(<1..368)
                     /locus_tag="VI195_10115"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6824785.1"
                     /translation="MGTARLLVNELCALLDLHTRGLLMQRWLAPRDPARTAPGVPPVL
                     FVHGILCNGGVWHRCLAALERRGVQNLFTLNLEPPLASVDCFAQQLVRRVEEVCRATG
                     AQRAIIVGHSLGGLVARACIA"
     gene            complement(996..2414)
                     /gene="glgA"
                     /locus_tag="VI195_10120"
     CDS             complement(996..2414)
                     /gene="glgA"
                     /locus_tag="VI195_10120"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY6824786.1"
                     /translation="MRVLIASSEIQPLAKTGGLADVCAGLASELSRLGAEVRLILPAY
                     ESALDVVREPGMPTDLGELLGVPARLILARVPGNGLAIWLVDCPALFRRPGTPYQDAQ
                     GADWPDNALRFGVFSHAIARVALGAATLDWRADIVHCHDWHTGLAPYLIAGRAERPHS
                     VFTIHNAAFQGNFPLSVAPDLGLPREVLTPETMEFYGRLSFLKAGIRYAERLTTVSPT
                     YARELLTPEFGCGLEGLIATRASDFIGILNGIDTRTWNPATDPLIAERYSSQDLAGKA
                     ACKAELQRENGLAQDARAPVVSFASRLTGQKMADTTLERLPRLLAQYPRLQFALLGSG
                     ERAIEEGFAELARRHPARVAAHIGYCEAREHRLHAGADILLHGSRFEPCGLAQMYAMR
                     YGAVPIVRGVGGLADTVADEDTGFVFREASPDALAEGIGRAVQAYERGEDWLPLQRRA
                     MNADFGWRRPARAYLDLYRSGA"
BASE COUNT          466 a          920 c          963 g          402 t
ORIGIN      
        1 gcgatgcagg cgcgcgccac caggccgccc aggctatggc cgacgatgat cgcgcgctgc
       61 gcgcccgtcg cccggcacac ctcctcgacc cggcggacaa gctgctgcgc gaagcaatcg
      121 acgcttgcca gcggcggctc gaggttcagc gtgaagaggt tttgaacacc ccggcgctcg
      181 agcgccgcca agcatcggtg ccatacgccg ccattgcaca ggatgccatg cacgaaaagc
      241 acgggcggca cgcccggcgc tgtgcgggcc ggatcgcgcg gcgcgagcca gcgctgcatg
      301 agcagacccc gcgtatgaag atcgagcagc gcacagagtt cgttcacgag cagccgcgcg
      361 gtgcccatgg ccccgagcgg cgagctctcg gcagcatgcg catgccgcga gcgcaggcag
      421 agccaaacca gcaatgcgag acgccagccg aggaagatcg tcaacgcgac cagcgcgctc
      481 actgcgaccg acacgccatg gcttgcggca agccagcagg ccatcgcata gagcaagagc
      541 tccgcaagca gcgccacgag catggcgcgc gtcatcattg gagacccgcc tgccgatcag
      601 tgacgcgcgg caagcaagcc gatgatcacc cctgccgagt aaaagcgccg tcccggcgcg
      661 atgcactggg atgagggaga atggtggtac accggcacgg gacggcacaa atgcatgaag
      721 tgatgttcac gcttcgctgc ccgcatcgtt tgacgcgcgt caatcccgtg cgacggcaca
      781 aataagaagc ccgccaccag ttcaggaagg cgggctgggg tggagctgcc ggtccggtgc
      841 ctttggacct ccggccggtc tgagcgtcgt ctccatccga cggacgctag gagcgtacgt
      901 tctcgcccgg tttccgggcc gctcatcttt gcgcctcccg tagggagtgc gctattgaat
      961 gctagcggat gcggcggcga gcgttttgac cggcgtcaag cgccgctgcg gtagagatcc
     1021 aggtatgcgc gtgccggacg gcgccagccg aagtcagcat tcatagcgcg tcgttgcagc
     1081 ggcagccagt cctcgccgcg ctcgtaggcc tgcacggcgc ggccgatgcc ctcggcgagc
     1141 gcgtcgggac tcgcctcgcg aaacacgaag ccggtgtcct cgtccgccac ggtgtcggcg
     1201 aggccgccga cgccgcgcac gatcggcacg gcgccgtagc gcatcgcgta catctgcgcg
     1261 agcccgcagg gctcgaagcg cgagccgtgg agcaggatgt cggcacctgc gtgcaggcga
     1321 tgctcgcgcg cttcgcaata gccgatatgc gcggcgacgc gggccggatg gcgccgcgcc
     1381 agctcggcga aaccctcctc gatcgcgcgc tcgcccgagc cgagcaaagc aaactgcagg
     1441 cgcgggtatt gcgcgagcag ccgcggcagc cgctcgagcg tggtgtccgc catcttctgc
     1501 ccggtgagcc ggctcgcgaa cgacaccacg ggtgcccgcg catcctgcgc aagaccgttc
     1561 tcgcgctgca gctcggcctt gcaggccgcc ttgccggcga gatcctggct ggaatagcgc
     1621 tcggcgatga gcgggtcggt ggccgggttc caggtgcgcg tatcgatgcc attcaggatg
     1681 ccgatgaagt cgctcgcgcg cgtggcgatg aggccctcga gcccgcagcc gaattcgggg
     1741 gtcagcagct cgcgcgcata agtcgggctc acggtcgtca gccgctcggc gtagcggatg
     1801 cccgctttca ggaacgagag ccgcccgtag aactccatgg tctcgggggt gagcacctcc
     1861 cgcggcaagc cgaggtccgg cgcaaccgac agcgggaagt tcccctggaa cgcggcgttg
     1921 tggatggtga atacgctgtg cggccgctcg gcgcgaccgg cgatcaggta cggcgcgaga
     1981 ccggtgtgcc agtcatggca atgcacgatg tcggctcgcc agtcgagtgt ggcggcgccg
     2041 agcgccacgc gcgcgatcgc gtggctgaag acgccgaagc gaagggcgtt gtccggccag
     2101 tcggcgcctt gtgcatcctg gtacggtgtg cccggccggc gaaaaagcgc cgggcaatca
     2161 accagccaga tcgcgaggcc gttgcccggc acgcgcgcga ggatcaggcg cgccggcacg
     2221 ccgagcagct cgccgagatc cgttggcatg cctggttcgc gcacgacatc aagcgccgat
     2281 tcgtacgccg gcagtatgag ccggacttct gcgccgaggc gcgagagctc gctcgcgagg
     2341 ccggcgcaaa cgtcggcgag cccgcccgtc ttggcaagcg gctggatctc ggatgaggcg
     2401 ataagcacgc gcatcgagtg ctcgtgctgc ccttttaacc gcgcgccgta gttgggcgtt
     2461 gatgcacgtc aacgggctgc tccggcgggc ggtgtgcaat tgaaattgcc atgtacgaat
     2521 cgccgctcga atggtgtcct gcgtgtcagg aatgggtagc gcttgaccag agcctcgctg
     2581 aacgcacgcg cacgaaaggc tgttcgccgg agcgttgtcc gctcgcgcgc cgtttcatcg
     2641 gcgaggccga gctgctcgcg cgcccgccgc gcgcgggtgc ggagcaacgg tgatggcggg
     2701 caatccgggc gcactcaccc tcgtgctgct ctggcacatg caccagcccg a
//
LOCUS       DATDMP010000576         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_647060, whole genome shotgun sequence.
ACCESSION   DATDMP010000576 DATDMP010000000
VERSION     DATDMP010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647060"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="VI195_10125"
     CDS             complement(<1..2002)
                     /locus_tag="VI195_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY6824787.1"
                     /translation="MMRVRSAAHSHADLIGNLIRRHGTEPPIPDVVRSSWSRCLNNYA
                     LDPQETRRPQSVEPTDLQARRERLGVLLAIARIEMEALGRLIKHSEFSIMLTDRDGVI
                     LSYIGDTGFSATARRCGFREGVTWSEREMGTNGMGTCLTARRPVVIHRMDHFLTQNTA
                     LTCAAAPIFDMRGELLAALDISGASSNPQSHTLALVEMAAQNVENRTLLSACKDYHVV
                     RFHRCSEFVSTPGEGVLAFDEQGLVRGANRAALALLEHRDHESVCGQRIDRILDTSLA
                     TLMRLSERSAFPPEPLGSKLAERRWFATVHAPASSPRRSQAAGAAAAAAGASPGAAPG
                     APGHGSLECLHTSDPVMRHNVEVLQRVVDRDISVLLLGETGTGKGYCARAIHNASNRA
                     DKPFVTVNCAAIPETLIESELFGHKAGAFTGATREGGMGRILQANGGTLFLDEIGDMP
                     VALQTRLLNVIEDREVMPLGGSKVVSVDVRVISATQHQPLDMIATGRFREDLYYRLNG
                     ITVRLPPLRQRSDCAELIAKLLHSEAGSGPAVEIEPALLERLVRHSWPGNVRQLRNVL
                     RTMLALRTSDCLALADFNEQWLAFGAAEPAPPAEPAEPAGSDGESVLSGAERAALRRM
                     LESCHWNVSAAAARRHISRRTIYRKIHRHGLLRHAQHLGAGTQ"
     gene            complement(2037..>2396)
                     /locus_tag="VI195_10130"
     CDS             complement(2037..>2396)
                     /locus_tag="VI195_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6824788.1"
                     /translation="NPYKDSDATIVAEGNRLFMSYACSGCHGGTGGGGMCPPVTNDIW
                     VYGGDDDTLFRLVTYGSVVLQSKGYSRTAQENVVAPMPPMGGVVKTADDLWRILAFIR
                     ANFHGSPSCKFGCKPGG"
BASE COUNT          339 a          831 c          846 g          380 t
ORIGIN      
        1 cctgcgtgcc cgccccgagg tgctgggcgt gccgcagcaa cccgtgccga tgaatcttgc
       61 gatatatggt ccggcggctg atgtgccgcc gcgcggccgc cgcactgacg ttccagtgac
      121 aggactcgag catgcgccgc agcgcggcgc gctccgcgcc gctcagcacg ctctcgccgt
      181 cgctcccggc cggctcggcc ggctcggccg gcggtgccgg ctcggcggcc ccgaacgcga
      241 gccactgctc gttgaagtcg gcgagcgcca ggcagtccga ggtgcgcagc gcgagcatgg
      301 tgcgcagcac gttgcgcagc tgccgcacgt tgcccggcca gctgtggcgc accagccgct
      361 cgagcagcgc cggctcgatc tcgacggccg gcccgctgcc cgcctcgctg tgcagcagct
      421 tggcgatcag ctcggcgcag tcgctgcgct ggcgcagcgg cggcaggcgc accgtgatgc
      481 cgttgaggcg gtagtaaaga tcctcgcgga aacgcccggt ggcgatcatg tcgagcggct
      541 ggtgctgcgt ggcgctgatt acgcgcacgt cgacgctcac caccttgctg cccccgagcg
      601 gcatgacctc gcggtcctcg atcacgttca gcagccgcgt ctgcagcgcc accggcatgt
      661 cgccgatctc gtcgaggaac agcgtgccgc cgttcgcctg caggatccgt cccatgccgc
      721 cttcgcgcgt ggcgccggtg aaggcgcccg ccttgtggcc gaacagctcg ctctcgatga
      781 gcgtctcggg gatcgctgcg cagttcaccg tgacgaaggg cttgtcggcc cggttgctgg
      841 cgttgtggat ggcccgcgcg caatagccct tgcccgtgcc ggtttccccg agcagcagca
      901 ccgagatgtc gcgatcgacc acgcgctgca gcacctcgac gttgtgccgc atgaccgggt
      961 cggaggtatg caggcactcg agcgagccgt ggcccggtgc gccgggcgcg gcgcccggcg
     1021 aggcgccggc cgcggcagcg gctgcgcccg cggcctggct gcggcgcggc gacgacgccg
     1081 gcgcatgcac ggtcgcgaac cagcgccgct cggcgagctt ggagccgagc ggttcggggg
     1141 ggaaggcgct gcgctccgag agccgcatga gcgtcgcgag gctggtatcg aggatgcgat
     1201 cgatgcgctg gccgcacacc gactcgtggt cgcgatgctc cagcagcgcg agcgcggcgc
     1261 ggttcgcgcc ccgcaccagg ccctgctcgt cgaacgccag caccccttcg cccggagtgc
     1321 tcacgaattc cgagcagcgg tggaagcgca ccacatggta gtccttgcag gcgctcagca
     1381 gagtgcggtt ctcgacgttc tgcgccgcca tttccaccag cgccagggtg tggctctggg
     1441 gattggagga ggcgccggag atgtcgagcg ccgcgagcag ctccccgcgc atgtcgaaga
     1501 tcggcgccgc cgcgcaggtg agcgcggtgt tctgcgtgag gaaatgatcc atgcggtgga
     1561 tcacgaccgg gcgccgcgcg gtcaggcagg tgcccatgcc gttggtgccc atctcgcgct
     1621 cgctccaggt caccccctcg cgaaagccgc agcggcgcgc ggtggccgag aagccggtgt
     1681 cgccgatgta gctgaggatc accccgtcgc ggtcggtgag catgatgctg aattcggaat
     1741 gcttgatgag cctgccgagg gcctccatct cgatgcgcgc gatggcgagc agcacgccga
     1801 ggcgctcgcg gcgcgcctgc aggtccgtag gctcgacgct ctgcggacga cgcgtctcct
     1861 gcgggtcgag cgcgtagttg ttgaggcagc ggctccagga gctgcgcacc acatcgggga
     1921 tcggcggctc ggtcccgtgc cggcggatca gattgccgat gaggtcggcg tgactatgag
     1981 cagcggaccg cacgcgcatc atggctcaag gctcctcaca agcatcgggc cgccccctaa
     2041 cccccgggct tgcagccgaa cttgcagctc ggactgccgt ggaagttggc ccgtatgaat
     2101 gcaaggatgc gccataaatc gtcggccgtc tttactaccc cccccatggg cggcatcggg
     2161 gccacgacat tctcctgggc ggtacggctg tagcccttgc tctgcagcac gacgctgcca
     2221 taggtgacca ggcggaacag cgtgtcgtcg tccccgccgt agacccagat gtcgttggtg
     2281 accggcggac acatgccccc gcccccggta ccgccgtgac agccgctgca ggcgtagctc
     2341 atgaaaagcc tgttgccctc ggcgacgatg gtcgcgtcgc tatccttata tgggtt
//
LOCUS       DATDMP010000577         4941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_869253, whole genome shotgun sequence.
ACCESSION   DATDMP010000577 DATDMP010000000
VERSION     DATDMP010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869253"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VI195_10135"
     CDS             <1..427
                     /locus_tag="VI195_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY6824789.1"
                     /translation="DVFISTAAVADYRPARAAEQKIKKTSDTLELAMERTADVLATVA
                     ARSERPFVVGFAAETESVEQNARSKLLKKNLDMIAANEVGHAKAFDSEENQLIVLTRS
                     GRHELGRADKLTLARGLIALIAQEMAARGGARKRGTALA"
     gene            424..900
                     /gene="dut"
                     /locus_tag="VI195_10140"
     CDS             424..900
                     /gene="dut"
                     /locus_tag="VI195_10140"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY6824790.1"
                     /translation="MSDTRAPRCALQVRILDARLGTEFPLPAYATAGSAGLDLRACLE
                     APLALEPGRAELIPTGLAIHLSDPGLAALILPRSGLGHRHGIVLGNLVGLIDSDYQGQ
                     LMVSCWNRAAEPFTIRPGERIAQLVVVPVVQVQLDVVTEFSASARGAGGFGHSGRH"
     gene            complement(934..1605)
                     /locus_tag="VI195_10145"
     CDS             complement(934..1605)
                     /locus_tag="VI195_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6824791.1"
                     /translation="MLLATAALGTSAWSAPSSQTSQNAHKGVAYRWVDDQGVVHYGDN
                     IPPQYANQDRTILNSQGVEVGHLDKQKTAEEEAAASHARDALMKQKQHDAFLVSTYTS
                     VKDIEALRDVRLDQLRGQRAAAEQYLESLRARLSSLQSRALGYRPYSTRADAWRMPDD
                     LAENLVHTLNELRVQDSALATKGAEETALRAQFQSDIERYRELHAIHSQSAAIPTARA
                     AAAAE"
     gene            complement(1675..2334)
                     /gene="pyrE"
                     /locus_tag="VI195_10150"
     CDS             complement(1675..2334)
                     /gene="pyrE"
                     /locus_tag="VI195_10150"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY6824792.1"
                     /translation="MQQYQLTFIELALRRNALRFGRFTLKSGRESPYFFNAGLFSDGE
                     SAAALGSCYAAAIVASGLAFDMLFGPAYKGIPLATATAIALSVDHGVNVPYAYNRKEA
                     KAHGEGGSIGGAPLERRVLIVDDVITAGTAVRESLGLIRAAGAQPVGVALALDRQERG
                     QGELSAVQEVEQLHGLPCVSIVTLADLIEALTVPHNGRARISDDQLTALRAYRERYGI
                     C"
     gene            2351..3118
                     /locus_tag="VI195_10155"
     CDS             2351..3118
                     /locus_tag="VI195_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY6824793.1"
                     /translation="MRVITVNVCGIRSAAEKGLFRWLRRQRADYICLQETKSQEHQLA
                     GHDVHLFGYHRFFCDAERKGYAGVALYSRLPPERVLRGFGVDEFDREGRYLEVQVGAL
                     SVVSLYLPSGSAGPERQASKFRFLETFTPHLARLRRRGRHYILCGDWNIAHRQIDLKN
                     WRSNQKNSGFLPEERAWMDRLFGTAGYIDAFREVRPEAELYTWWSNRGQARTNNVGWR
                     IDYQVVSGSLAGKAHAASIYRQKRFSDHAPLIMDYRL"
     gene            3115..4518
                     /locus_tag="VI195_10160"
     CDS             3115..4518
                     /locus_tag="VI195_10160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6824794.1"
                     /translation="MSEGADRQANTAAGWLSALSIYRQPRVLAMLFLGFSAGLPFFLV
                     YSTLSAWLRQVGIQRSTIGMLAWVGILFSVKFLWAPIVDRVPPPLQRLLGRRRGWMLL
                     AQLGIALGLFNHSLCDPAGGVRPLALWALFTTFCAATQDIALDAWRIESAPLPMQGAM
                     LSAYQIGYRAALIVGGAGALAIAARAGWHASYGTMTALVSIGVLTTLLAPEPAGTAPA
                     ESVAREERVIAWMERRAHWPQGLRHAGEWFLTAVICPLTDFFGRYGMTLALLTLAFIS
                     VYRLAEFAMGSMVNPFYIDRGFTLTEIATVVKLVGPVMALIGVLLAGVLIARLGLLRS
                     LLLGSVLIILSNLGYALLASTPTPTLVGLGAVNALDNLALAMHGTALLAFLSGLTSAR
                     YTATQYALFSSLYALPGKILEGTSGMVVDHIGYPHFFLYTATLALPGLVLLYVLRQQG
                     AFERANARARTGAAAET"
     gene            4520..4903
                     /locus_tag="VI195_10165"
     CDS             4520..4903
                     /locus_tag="VI195_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6824795.1"
                     /translation="MTEELDLERVVCRLSDLTAAARGYTIGGGDWPLRLFVVRVAGKV
                     HGYLNRCPHAGHALDLLPQRFLTADGRLILCSSHGALFEKTTGYCVAGPCAGRSLTPV
                     PLRVQSGFVLLADSVDVEALASAAR"
BASE COUNT          765 a         1660 c         1624 g          892 t
ORIGIN      
        1 ggatgtgttc atctcgaccg ccgcggtcgc cgactaccgg ccggcgcgag cggcggagca
       61 gaaaatcaag aagaccagcg atacgctcga gctcgcgatg gagcgcaccg ccgatgtgct
      121 cgccaccgtg gccgcgcgca gcgagcggcc cttcgtggtc ggcttcgctg ccgagaccga
      181 atcggtcgag cagaacgcgc gcagcaaact gctgaagaag aacctcgaca tgatcgccgc
      241 caacgaggtc ggtcacgcca aggccttcga cagcgaggag aatcagctca tcgtgctcac
      301 ccgcagcggc cgccatgagc ttggccgggc agacaagctc accctggcgc gtggcctcat
      361 cgcgctgatc gcgcaggaga tggccgcccg gggcggtgcg cgcaagcgcg gtacggcgct
      421 cgcttgagcg acacgcgcgc gcctcgctgc gcgctccagg tgcgcatcct cgatgcgcgc
      481 ctcggcaccg agttcccgct gcccgcctac gctaccgcag gatccgccgg actcgatctg
      541 cgcgcctgtc tcgaggcgcc gcttgcgctc gagccgggga gagccgagct cattcccacc
      601 ggactcgcca ttcacctgag cgacccgggg ctcgccgcgc tcattctgcc gcgctcggga
      661 ctcggccatc gacacggcat cgtgctcggc aatctcgtcg gcctcatcga ctccgattac
      721 cagggtcagc tcatggtgtc gtgctggaat cgcgcggccg agcccttcac catccgcccg
      781 ggcgagcgca ttgcgcagct ggtggtggtc ccggtggtgc aggtacagct cgatgtggtg
      841 acggagttca gcgcttctgc ccgcggtgcc ggcggcttcg gccactccgg ccggcactga
      901 gcgcacgccg cgcgccggcc ggcggatccg agcttactcc gcggcggcag ccgcgcgcgc
      961 ggtggggatc gcggccgact gcgaatggat cgcatgcagc tcgcggtagc gctcgatatc
     1021 cgactggaat tgcgcgcgca gggcagtttc ctcggcgcct ttggtggcga gcgcgctgtc
     1081 ctgcacgcgc agctcgttca gggtatgcac cagattctcg gctaggtcgt ccggcatgcg
     1141 ccaggcgtcc gcgcgcgtgc tgtacgggcg gtagcccagc gcgcgcgact gcaggctcga
     1201 gagccgtgcc cgcagactct ccaggtactg ctcggctgcc gcacgctgac ccctgagctg
     1261 atcgaggcgc acgtcccgca acgcctcgat gtccttcacc gaggtatagg tggagaccag
     1321 aaatgcgtcg tgctgcttct gcttcatcag ggcatcgcgg gcatggctcg ccgccgcctc
     1381 ttcctcggct gttttctgct tatcgagatg accgacctcg acaccctggg agttgagaat
     1441 ggtgcgatcc tggttggcat actgcggcgg aatgttgtcg ccgtagtgca cgacgccctg
     1501 gtcatcgacc cagcgataag cgacgccctt gtgcgcattc tgcgatgtct gcgagctcgg
     1561 tgcggaccag gcgctggtgc cgagcgctgc ggttgcgagc agcaagagaa ccctgaggcg
     1621 cgaggcggga agcatatgtt ttatattccg acgctggagc gggcgctggc aagttcaaca
     1681 gataccataa cgctcccgat aggcgcgcag agctgtgagc tggtcgtcag aaatgcgcgc
     1741 tcgcccatta tgcggcacgg tgagcgcctc gatgagatcg gcaagtgtga cgatgctcac
     1801 acagggcagg ccatgcagtt gttcgacctc ctgcaccgca ctcaattcac cctgcccacg
     1861 ttcctgacga tcgagcgcga gcgcgacccc gaccggttgc gcgccggcag cacggatcag
     1921 gccgagcgat tcgcgcaccg cggtgccggc ggtgatcaca tcatccacga tcagcacgcg
     1981 gcgctcgagc ggcgcgccac cgatgctgcc gccttcgcca tgcgccttcg cctccttgcg
     2041 attgtaggcg tacggcacat tcacgccatg atcgacagag agtgcgatcg cagtggcggt
     2101 ggcgagggga atgcccttgt aggccgggcc gaacagcata tcgaaggcga ggccggacgc
     2161 cacgatcgcc gcggcgtaac agctgccgag cgccgccgcc gattcgccgt cgctgaacaa
     2221 acccgcgttg aagaagtagg ggctctcgcg tccggacttg agggtaaagc ggccgaagcg
     2281 cagcgcgttg cgtcgcagtg cgagttcgat gaaagtgagc tggtattgct gcatgccata
     2341 tcattgcgcc atgcgcgtga tcaccgtcaa cgtctgcggc atccgttccg cggctgagaa
     2401 ggggctgttc cgctggctgc ggcggcagcg ggccgattac atctgcctgc aggagaccaa
     2461 gagtcaggaa caccagctcg ccggacacga cgtgcacctc ttcggctacc accgcttctt
     2521 ctgcgatgcc gagcgcaagg gctacgcggg ggtggcgctg tactcgcgcc tgccgcccga
     2581 acgcgtgctg cgcggcttcg gggtcgatga gttcgaccgc gagggtcgtt acctcgaggt
     2641 gcaggttggc gccttgtcgg tggtgtcgct gtatctgccc tcgggctccg ccggtcccga
     2701 acgtcaggca tccaaattcc gctttctgga aaccttcacg ccgcatctcg cacggctgcg
     2761 ccggcgcggc cggcactaca tcctctgtgg tgactggaat atcgcccacc ggcagatcga
     2821 cctgaaaaac tggaggtcga accagaagaa ctctggtttt ctgcccgagg aacgcgcgtg
     2881 gatggaccgg ctgtttggca ctgctgggta catcgatgcg ttccgcgagg tgcgtccgga
     2941 ggccgagctg tacacctggt ggtcgaatcg tgggcaagca cgtactaaca atgtcgggtg
     3001 gcgcatcgac taccaggtcg tgagcggctc gctcgccggc aaggcacacg ccgccagcat
     3061 ctaccggcag aagcgcttct cagaccacgc accgctgatc atggactacc gcctttgagc
     3121 gaaggcgcgg accggcaagc taacaccgca gccggctggc tcagcgcact gtccatctat
     3181 cgccagccac gggtgctggc gatgttgttc ctcggcttct cggccggcct gccgtttttc
     3241 ctcgtgtact cgacgctctc ggcctggctg cgccaggtcg gcatccaacg cagcaccatc
     3301 ggcatgctgg cctgggtcgg aattctcttc tcggtcaagt tcctgtgggc gccgatcgtc
     3361 gaccgggtgc cgccgccgct gcagcgcctg ctcgggcggc gccgtggctg gatgctgctg
     3421 gctcaactcg gcatcgcact ggggctcttc aatcacagcc tgtgcgaccc ggccggcggt
     3481 gtgcgcccgt tggcgctgtg ggccctgttc accaccttct gtgccgccac gcaagacatc
     3541 gcgctcgatg cctggcgcat cgaatcggcg ccgctgccga tgcagggggc gatgctaagc
     3601 gcgtaccaga tcggctaccg cgcggcactc atcgtcgggg gcgccggggc gctggcgatc
     3661 gccgccaggg cgggctggca cgcgagctac ggcaccatga cagcgctggt gagtatcggt
     3721 gtcctcacca cgctgctcgc gcccgagccc gccggcacag cgccggcgga gtcggtcgcg
     3781 cgcgaggagc gtgtgattgc gtggatggag cgtcgtgcgc actggccgca gggactgcgg
     3841 catgcgggcg agtggtttct gaccgcggtg atctgtccgc tgaccgattt cttcgggcgt
     3901 tatggcatga cgcttgcgct gctgacgctc gctttcatat cggtataccg tctcgcggag
     3961 ttcgccatgg gctccatggt gaatcccttc tacatcgacc gcggcttcac cctgacggag
     4021 atcgccacgg tggtgaagct cgtcggtccg gtgatggcac tcatcggggt actgctcgcc
     4081 ggcgtgctga tcgcccgact cggcctgttg cgatcgctgc tgctcggcag cgtactcatc
     4141 attctctcca acctcggcta tgcgctactc gcgagcacgc caacgccaac gctcgtcgga
     4201 ctcggcgccg tcaatgcact cgacaatctc gcgcttgcca tgcatggcac ggcgctcctc
     4261 gccttcctct cgggtctgac gagcgcacgc tataccgcca cacagtacgc gctgttctcc
     4321 tcgctgtacg cgctgccggg caagatcctc gagggtacct ccggcatggt ggtcgaccac
     4381 atcggctatc cgcacttctt cctctatacg gcgaccctcg ccctgccagg cctcgtgctg
     4441 ctctatgtgc tgcgccagca gggcgcattc gagcgcgcca atgcgcgcgc gcgcaccggg
     4501 gcggctgcag agacctgaca tgactgagga gcttgatctc gagcgagtcg tctgccggct
     4561 gtcggatctg acagccgcag cgcgcggata caccatcggc ggcggtgact ggccgctacg
     4621 gctcttcgta gtgagagtgg ccgggaaggt gcacggttac ctcaaccgct gtccccacgc
     4681 cgggcacgcg ctcgacctgc tgccgcagcg cttccttacc gccgatggca gactcatcct
     4741 gtgctcctcg cacggagcgc tgttcgagaa aaccaccggg tactgcgtgg ccgggccgtg
     4801 cgcgggacgc tcgctcacac cggtgccact gcgagtgcag agcggcttcg tgctgctcgc
     4861 cgacagcgtg gacgtcgagg cgcttgccag cgccgcgcgc tagcgcgtcg cccgaaattt
     4921 cctggctttc agctcgatgg c
//
LOCUS       DATDMP010000578         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1758236, whole genome shotgun sequence.
ACCESSION   DATDMP010000578 DATDMP010000000
VERSION     DATDMP010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758236"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VI195_10170"
     CDS             complement(<1..308)
                     /locus_tag="VI195_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824796.1"
                     /translation="MNAASWLESLPVQERWRALLLKDGPRVATWVLALALGVQAALIV
                     TDLAGAGRRGGAAAAPRPASASHALDLAAVTNAHLFGAAPVTRQDDANAPQTSVPLVL
                     T"
     gene            521..811
                     /gene="clpS"
                     /locus_tag="VI195_10175"
                     /pseudo
     CDS             521..811
                     /gene="clpS"
                     /locus_tag="VI195_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
     gene            874..3144
                     /gene="clpA"
                     /locus_tag="VI195_10180"
     CDS             874..3144
                     /gene="clpA"
                     /locus_tag="VI195_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY6824797.1"
                     /translation="MLSNELEYCLNDAFHQAREARHEYLTVEHLLLAILDTPKVREVL
                     RACGADLAKLKQELRDHIEQSTPRLEEGEEREVQPTLGFQRVLQRAVFHVQSSGKKEV
                     GVGNVLVAIFSEKQSHAVFLLNRQRVTRLDVVNYISHGLSKIAEEKTSEKDESSTDAE
                     REGEGGSALEKYTSNLNRMAQDGRIDPLIGRKLEVERTIEILCRRRKNNPLYVGEAGV
                     GKTAIAEGLARLIVEGQVPEVLSDCTLFALDMGALIAGTKYRGDFEKRLKGVISELKK
                     LPGAILFIDEIHTVIGAGAASGGVMDASNLIKPVLTNGEIRCIGSTTYQEYRHIFEKD
                     HALARRFQKIDVVEPSVAETIDILFGLKPRFEEHHGITYADEALRAAAELAARHINER
                     HLPDKAIDVVDEAGARARLKPVAEREATVEVRHIEDVVARMARIPAKSVSTSDREILK
                     NLERNLKLVIFGQDRAIEALAAAIKMARSGLGEARKPVGNFLFAGPTGVGKTEVTRQL
                     AIAMGVEFLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKHPHCVLLLDEI
                     EKAHPDVFNLLLQVMDHGTLTDNNGRKADFRHVIIVMTTNAGAQEMARPSIGFTHADN
                     ASDGMEAIRRIFTPEFRNRLDAVIQFAALDPVTIERVVDKLILEVESQLEQKGVSITL
                     DDAARRWIAEKGYDAKMGARPMARTIQEHIKRPLAEELLFGRLAGGGHVRVSVAPDGA
                     ALALTCEPAQSPTVPA"
     gene            complement(3245..3463)
                     /gene="infA"
                     /locus_tag="VI195_10185"
     CDS             complement(3245..3463)
                     /gene="infA"
                     /locus_tag="VI195_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY6824798.1"
                     /translation="MAKEDAIQMEGEVVETLPNTTFRVKLKNGHVVTAHISGKMRKNY
                     IRILTGDAVTVEMTPYDLTKGRIIYRGR"
BASE COUNT          693 a         1132 c         1125 g          569 t
ORIGIN      
        1 gtgagcacga gcggaacact ggtctgtggc gcattggcgt catcctgccg ggtgaccggg
       61 gcggcgccga acaggtgcgc gttggtgacc gcggcaagat cgagggcatg gctcgccgag
      121 gccggccgcg gcgcggctgc cgcgccaccg cgccggcctg caccggcgag atcggtcacg
      181 atgagcgctg cctgcacgcc gagggcgagc gccagcaccc acgtggccac acgcggtcca
      241 tccttcagca gcagtgcgcg ccagcgctcc tgcactggca agctttcgag ccaggaagcg
      301 gcgttcatag acttaagcgc aagcgatcga tggtggtcca caacgaagcg ccggcacggt
      361 gtgcccagcc ttcgcccgcg caatcatacg aggctggcgc ttgtgctctc aagcaattgt
      421 ttctgcagga gtaactacgc cacattctgg caggcttgtg ttgccgtttc gcgaccccta
      481 taaagacacc atgcccgatc tgcatccggt ccgccccgaa agcggtgtgc tcgtcgaaga
      541 ggcgaaaccg gaggtcaaac aaccgcctct ataccgtgta gtactgctga atgacgacta
      601 caccccaatg gagtttgtgg tcgatgtgct ggaaaagttc tttacgatga atcgtccgac
      661 cgccacacgc atcatgctcg aggtccatac caagggtaag ggggtgtgtg gggtcttcac
      721 atacgagatc gcggaaacta aggtggcgca ggtgaccacc tacgcgcgcg accaccagca
      781 cccgctgatg tgcacgctcg aggaggcctg agcgggctgc gctgcgaagg atgatcgacc
      841 ggaactcgac gcgcaattga aaggttcgat gaagtgttat ccaacgagct cgaatactgc
      901 ctcaacgacg cattccatca ggcgcgcgag gctcgccacg agtacctgac cgtcgagcac
      961 ctgctgctcg ccattctcga cacccccaag gtgcgcgagg tgctgcgcgc ctgtggggcc
     1021 gatctcgcca agttgaagca ggagctgcgc gatcacatcg agcagtccac accgcgtctg
     1081 gaggagggcg aggaacgcga ggtgcagccg acgctcggct tccagcgcgt gttgcagcgg
     1141 gccgtgttcc acgtgcagtc gagcggcaag aaagaggtcg gcgtcggcaa cgtgctggtg
     1201 gcgatcttca gcgaaaagca gagccacgcg gtgttcctgc tcaatcgcca gcgcgtgacg
     1261 cgcctggacg tcgtcaacta catctcgcac gggctgtcga aaatcgccga ggagaagact
     1321 tcggagaagg acgaatcgtc caccgacgcc gagcgtgagg gtgagggcgg aagcgcgctc
     1381 gagaagtaca ccagcaacct caaccgcatg gcacaggacg gacgtatcga cccgctcatc
     1441 gggcgcaagc tcgaggtcga gcgcaccatc gagatcctct gccgccgccg caagaacaac
     1501 ccgctgtatg tcggcgaggc cggcgtgggc aagaccgcca tcgccgaagg actggcgcgg
     1561 ctcatcgtcg agggccaggt gccggaggtg ctctccgact gcacgctgtt cgcgctcgac
     1621 atgggagcgc tcatcgccgg caccaagtac cgcggggact tcgagaagcg cctcaagggc
     1681 gtgatcagcg agctgaagaa gctccccggg gccatcctgt tcatcgacga gatccacacc
     1741 gtgatcggtg ccggcgcggc ctcgggcggc gtcatggatg cctccaatct catcaagccg
     1801 gtgctcacca acggcgagat ccgctgcatc ggctcgacca cctatcagga gtaccgtcac
     1861 atcttcgaga aggaccacgc gctcgcgcgg cgcttccaga agatcgacgt tgtcgagccc
     1921 tcagtggcgg agaccatcga cattctcttc gggctgaagc cgcgcttcga ggagcaccac
     1981 ggcatcacct acgcggacga agcgttgcgt gccgctgccg agctcgccgc gcgccacatc
     2041 aacgagcggc atctgccgga caaggccatc gacgtggtcg acgaggccgg cgcgcgcgcg
     2101 cgcctcaagc ccgtggccga acgcgaggcg accgtcgaag tgcgtcacat cgaagatgtg
     2161 gtcgcgcgca tggcgcgcat tccggcaaag agcgtctcga cctccgaccg ggagatcctc
     2221 aagaatctcg agcgcaatct gaagctcgtc atcttcggac aggaccgcgc catcgaggcg
     2281 ctggccgctg ccatcaagat ggcacgctcg ggtctcggtg aggcgcgcaa accggttggc
     2341 aacttcctgt tcgccggccc gaccggtgtc ggcaagacgg aggtgacacg ccagctggcc
     2401 attgccatgg gcgtggagtt cctgcgcttc gacatgtccg agtacatgga gcgccacacc
     2461 gtctcacgcc tgatcggcgc tccgccgggc tacgtcggct tcgatcaggg tgggctgctg
     2521 acggaagcga tcaccaagca cccacactgc gtactgctgc tcgatgagat cgagaaggcg
     2581 cacccggacg tgttcaacct gctgctgcag gtgatggatc acggcacgct caccgacaac
     2641 aacgggcgca aggccgactt ccgccatgtc atcatcgtca tgaccaccaa cgccggtgcg
     2701 caggagatgg cgcgcccctc gatcggcttt acgcacgcgg acaacgccag cgatggcatg
     2761 gaggcgatcc gccgcatctt cactcccgaa ttccgcaacc gcctcgacgc ggtcatccag
     2821 ttcgccgcac tcgatccggt caccatcgag cgcgtggtgg acaagctgat cctggaagtg
     2881 gaatcgcagc tcgagcagaa gggcgtcagc atcacgctcg atgacgcggc ccggcgctgg
     2941 atcgccgaga agggctacga cgcgaaaatg ggtgcgcggc ccatggcacg cacgatccag
     3001 gagcacatca agcgaccgct cgcggaagag ctgctgttcg gacggctcgc cggtggcgga
     3061 cacgtgcgcg tgtcggtcgc gccggatggc gcagcgctcg ccctgacgtg cgagccagca
     3121 cagtcgccga ccgtgccggc ctaaaccacc ccacgaaatt gcgcttgccg cgagcagcag
     3181 gcgcacaggg atcaaacaag acgcgcgatt tcgcggggac cccggtttgg gaggggaaca
     3241 agccttaccg gccgcggtag atgatgcggc ccttggtcaa atcgtagggt gtcatttcga
     3301 ccgtgaccgc atcccctgtc aggatgcgga tgtagttctt gcgcatcttg ccggagatgt
     3361 gcgcggtcac cacgtggcca ttcttcagct tcacccggaa agtcgtgttg ggcagggtct
     3421 cgacgacctc gccttccatc tggattgcat cttctttagc catagaccct acgcggcccc
     3481 aaggggcgcg aatcatgcat aaaggacccg cccattgca
//
LOCUS       DATDMP010000579         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_314327, whole genome shotgun sequence.
ACCESSION   DATDMP010000579 DATDMP010000000
VERSION     DATDMP010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314327"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VI195_10190"
     CDS             <1..431
                     /locus_tag="VI195_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824799.1"
                     /translation="DLLRSRAERLRALSVSFLPELVGRLERSEHRLLSAGPHLPVLCL
                     ARPAVLWLRATRARRRLHRYVDTALRAAAADSAAVAGQRTRLRQTACAYVDRRLLATR
                     RVAGFQGYERLLSIWHAVHVPLLFILIVAGVVHVVAVHLY"
     gene            complement(462..830)
                     /locus_tag="VI195_10195"
     CDS             complement(462..830)
                     /locus_tag="VI195_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824800.1"
                     /translation="MADEQDYQTQISEQGGILRVVVRGKRELGRSRRLVALIRKTAEE
                     HGCRRVLLDTSALSEPPPNDYRFALGELLATEWRGLRVAVINPLEAGHGFVETVAVNR
                     GATTRIFRTEDSAVEWLGID"
     gene            complement(858..1226)
                     /locus_tag="VI195_10200"
     CDS             complement(858..1226)
                     /locus_tag="VI195_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824801.1"
                     /translation="MADEQDYETRITEEGGILRFVPRGKLEFGRSERFLTLVRNTARA
                     RGCRRVLIDARAFSDPMPNNDRFAIGERVAEEWRGLRVAVVSPVEARDGFIETVAVNR
                     GATARGFESVERAMDWLSLE"
     gene            1348..>2331
                     /locus_tag="VI195_10205"
     CDS             1348..>2331
                     /locus_tag="VI195_10205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6824802.1"
                     /translation="MPDERHQPSPRPPPQLRHRIVIVGGGAGGLELATKLGDRFGRRG
                     RAQIELIDESRAHLWKPLLHEIAAGSMDLSVHELNYLAQSHWHHFRYRLGEMIGLDRQ
                     RREVLVAPVFDEDGSEITPGRTVGYDTLVIAVGSLTNDFGTPGVKEHAIALETPGDAA
                     RFHRRLVNALMRAHAQREPLRPEQLQVAIIGAGATGVELAAELHNTTRELVAYGLDRI
                     DPDQDIKLNVIEAADRILPALPERLSEAAARLLESKGVRVRTAARVAEVLPAGVRLAS
                     GELIAAELVVWAAGVKAPDFLGNLDGLETNRANQLVVKQTLQSTRDESIFAI"
BASE COUNT          337 a          856 c          784 g          354 t
ORIGIN      
        1 tcgacctgct gcgctccagg gcggagcggt tgcgcgccct gtcagtgagt ttcctgcccg
       61 agctcgtcgg tcgcctggag aggtcggagc accgcctgtt gtcggcgggt ccccatctgc
      121 cggtcctgtg cctggcgaga cctgcggtac tgtggctgcg cgcgacccgc gcccgccggc
      181 ggctgcatcg ttacgtcgac accgcgctgc gcgccgccgc tgccgactcg gccgcggttg
      241 ccgggcagcg gacgcgattg cgccagaccg cctgcgctta cgtcgaccgg cgcctgctcg
      301 cgacacgccg cgtcgccgga ttccagggat acgagcggtt gctctcgatc tggcacgcgg
      361 tgcacgtgcc gctgctgttc atcctgatcg tggccggcgt ggtgcacgtg gtcgcggtcc
      421 acctgtacta gtggtccgca cggcggtgcc gggcggcgtg actaatcgat tcccagccac
      481 tccacggcac tgtcttcggt cctgaagatc cgtgtcgtgg ccccgcgatt gacggcaacg
      541 gtctcgacga agccgtgccc cgcctcgagc gggttgatca ccgccacacg aagcccgcgc
      601 cactccgtgg caagcagctc accgagcgcg aagcgatagt cgttgggcgg gggttcgctg
      661 agcgcgctgg tgtcgagcag cacccgccgg caaccgtgct cctcggcagt cttgcgtatc
      721 agcgcgacca gacgccggct gcgccccaat tcgcgcttcc cgcgcaccac cacccgcagg
      781 atgccgccct gttcgctgat ctgagtctgg tagtcctgct cgtccgccat atccgcgcga
      841 gccccccccg ggccggatca ctccaagctc agccagtcca tggcgcgttc tacggactcg
      901 aaaccgcgcg cagtcgcccc gcgattgacc gcgaccgtct cgatgaaacc atcgcgcgct
      961 tcgaccggac tcacgaccgc aacccgcaag ccgcgccatt cctcggcgac ccgctcgccg
     1021 atcgcgaagc ggtcgttgtt cggcatcgga tcggagaagg cgcgcgcatc gatcagcacc
     1081 cgccggcagc cgcgcgctct tgccgtgttg cgcaccagcg tcaggaaccg ctcggaccgg
     1141 ccgaactcga gcttcccgcg tggcacgaag cgcagaatgc cgccctcctc ggtgatccgg
     1201 gtctcgtagt cctgctcgtc cgccatagtc ctgcacggcg cgccgcggcc tgctgccgca
     1261 gccgccgtgc gccctcccga tgagacgcgc actccattgc atcctacact gtggggtaaa
     1321 acacgccagc cccgcgcgca gccacccgtg cccgacgaac gccaccagcc gagccctcgc
     1381 ccaccgccgc agttgcggca ccggatcgtc atcgtcggcg gcggggcggg cgggctcgag
     1441 ctcgccacca agctcggtga ccgcttcggc cggcgtggcc gggcgcagat cgagctgatc
     1501 gacgagtccc gcgcccacct gtggaagccg ctgctgcacg agatcgcggc cggcagcatg
     1561 gatctgtcgg tgcacgagct caactacctg gcccagtcgc actggcacca tttccgctac
     1621 cgcctcggcg agatgatcgg gctcgaccgg cagcggcgcg aagtcctggt ggcaccggtg
     1681 ttcgatgagg acggcagcga gatcaccccc gggcgcacgg tcggctacga cacgctggtg
     1741 atcgccgtcg gcagtctcac caacgacttc ggcacccccg gcgtcaagga gcacgcgatc
     1801 gccctcgaga cgccgggcga tgccgcgcgc tttcatcgcc gcctggtcaa cgcgctcatg
     1861 cgggcgcatg cgcagcgcga gccgctgcgt cccgagcagc tgcaggtcgc gatcatcggc
     1921 gccggggcca ccggcgtcga gctcgcggcc gagctgcaca acaccacgcg ggaactggtg
     1981 gcctacgggc tcgatcggat cgatcccgac caggacatca agctgaacgt gatcgaggcg
     2041 gccgaccgca tcctgccggc gctgccggag cggctgtccg aggcggcggc caggctgctc
     2101 gagagcaagg gcgtgcgcgt gcggaccgcc gcgcgcgtcg ccgaggtgct gcccgccggg
     2161 gtgaggctcg cgagcgggga gctcatcgcg gcggagctgg tcgtctgggc ggcgggcgtg
     2221 aaggcgccgg atttcctcgg caacctggac ggcctcgaga ccaaccgcgc gaaccagctg
     2281 gtggtgaagc agacgctgca gagcacgcgc gatgagagca tttttgcgat c
//
LOCUS       DATDMP010000580         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1092438, whole genome shotgun sequence.
ACCESSION   DATDMP010000580 DATDMP010000000
VERSION     DATDMP010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092438"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..866
                     /locus_tag="VI195_10210"
     CDS             222..866
                     /locus_tag="VI195_10210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY6824803.1"
                     /translation="MSDTTLVIGPTVLAGRHVRLEPLAHTHIPGLVAAAREDPELYRW
                     SYIPQGVMEMAQYVDTALSGREAGTALPFATVRTGDDAVMGSTRFFDMERWAWPAGHP
                     DARRGGLDACEIGYTWLSASAVRSAANTESKLLMLGHAFEVWSAHRVCFHTDVRNERS
                     RAALERIGAQFEGVLRAHRLAADLTPRDSARYSIVAAEWPQLKQRLQDRLAARR"
     gene            complement(903..>2033)
                     /locus_tag="VI195_10215"
     CDS             complement(903..>2033)
                     /locus_tag="VI195_10215"
                     /inference="COORDINATES: protein motif:HMM:NF036494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6824804.1"
                     /translation="NRPPLPRASVLPQIKDPVLAYGFPTGGTTLSITRGIVSRIEFVP
                     YGVSTSGLRIQIDAAINPGNSGGPAISDNRMIGLAFSHLANSENISYIIPNEEIELFL
                     KGVGGGHYDGKPGMYDDLQTLENPALRGYLKLEPAVRGMIVHRADDSSPGYPLKQWDV
                     ITHIGTEPIDDEGMVAIRNNLRVGFAYLIQHIARDGTVPLTVVRAGKTQQIHMPVSAH
                     RPLLIDSLQGEYPSYFILGPIVFERASIESLQLIRSHGQMAFGNPLVARFGDRPDADR
                     QELVILPAPLFPHALSKGYSDPTGCVVKSVNGTPVRSLAHLVALLRDLKDEYLTLDFD
                     NRASEAMVFPRAQLVAATEAILTDNGVRAQGSPDMMKIWENR"
BASE COUNT          325 a          653 c          718 g          337 t
ORIGIN      
        1 cccggcgggc accggcttcg ccgctcatgc ctcgcgccgc aggaagatgg agggcaccga
       61 ccgccgcagc ttcatggatg tcggcggcgg gctgccggac accgaggagt cgagcgtcgg
      121 cgaggagtcg gagagcgcgg cgagctgatg ccgaaagggg ccggcggctc ttcgccggcc
      181 ctttttccgc tctgcagagg ggctcgcagg tccagcgcac tatgagtgat accacgctcg
      241 tcatcggacc gacggttctc gccggccggc acgtacggct cgagccactg gcacacaccc
      301 atattcccgg actggtcgcg gcggcacgcg aggacccgga gctctaccgc tggagctaca
      361 ttccccaggg cgtgatggaa atggcgcagt acgtcgacac tgcgctcagc gggcgcgagg
      421 cgggcacggc actgccgttt gcgaccgtac gcaccgggga cgatgccgtc atgggctcga
      481 cccgattctt cgacatggag cgctgggcgt ggcccgcggg ccacccggat gcgcgccgcg
      541 gcggtctcga tgcctgcgag atcggctaca cctggttgtc ggcctccgcg gtgcgcagcg
      601 cggcaaacac ggaatcgaag ctcctcatgc tcggccatgc gttcgaggtg tggagcgcgc
      661 accgggtgtg cttccacacc gatgtgcgca acgagcgctc gcgcgcggcg ctcgagcgca
      721 tcggcgcgca gttcgagggc gtgctgcggg cccatcgcct ggctgccgat ctcacaccgc
      781 gcgattcggc gcgctactcg atcgtggcgg ccgaatggcc gcagctgaag cagcgcctgc
      841 aggatcggct cgcggcgcgg cgctgagctc ccgcaggcgc cgcggaccgc gcgagcgggc
      901 gcctagcgat tttcccagat cttcatcatg tccggggagc cctgggcgcg caccccgttg
      961 tcggtgagta tcgcctcggt ggcggctacc agctgcgcgc gcggaaacac catggcctcg
     1021 ctcgcgcggt tgtcgaaatc cagcgtgagg tactcatcct tcaggtcgcg cagcagcgcc
     1081 accaggtgtg cgaggctccg caccggcgtg ccgttgacgg acttcaccac gcagccggta
     1141 ggatccgagt agcccttgga gagcgcgtgt gggaacagcg gcgcgggcag gatgaccagc
     1201 tcctggcgat cggcgtccgg gcggtcgccg aatctcgcca ccagcggatt gccgaacgcc
     1261 atctggccgt gcgagcggat cagctgcagt gactcgatcg aagcacgctc gaacacgatc
     1321 ggtccgagga tgaagtagga cggatattcg ccctgcagcg aatcgatgag cagaggccgg
     1381 tgcgcggaca ccggcatgtg gatctgctga gtcttgccgg cgcgcaccac ggtgaggggc
     1441 accgtgccgt cgcgcgcgat gtgctggatg agataggcga agccgacgcg caggttgttg
     1501 cggatcgcaa ccatgccttc atcgtcgatc ggctcggtgc cgatatgcgt gatgacatcc
     1561 cactgcttga gtggataacc cggactgctg tcgtccgcgc ggtgcacgat catgccgcgc
     1621 actgcaggct cgagcttcag gtagccgcgc agcgccggat tctcgagcgt ctgcaggtcg
     1681 tcgtacatgc ccggcttgcc atcgtagtgg ccgccgccga cacctttgag gaacagctcg
     1741 atctcctcgt tcgggatgat gtagctgatg ttctccgagt tcgccagatg gctgaaggca
     1801 agtccgatca tgcggttatc cgagatggca ggtcccccgc tgttgccggg attgatggcg
     1861 gcatcgatct ggatgcgcag ccccgaggtc gagacgccgt aggggacgaa ctcgatgcgt
     1921 gagacgatgc cgcgggtgat cgagagcgtc gtgccgccgg tcggaaagcc gtaagccagc
     1981 accggatcct ttatctgcgg cagcacgctg gcgcgcggca gtggcggacg gtt
//
LOCUS       DATDMP010000581         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1425832, whole genome shotgun sequence.
ACCESSION   DATDMP010000581 DATDMP010000000
VERSION     DATDMP010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425832"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VI195_10220"
     CDS             <1..454
                     /locus_tag="VI195_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY6824805.1"
                     /translation="VWLAIGYFVPDPDTLQILEEAARRGVDVRLVLPGFSDFWAPIAA
                     ARSNYQPLLDAGVKIYEWRQALMHAKTAVIDGQWSSVGSTNLDWRSFVHNYEDDLLVF
                     DPAFGREMEKRYMADVGASTPIDPKTWANRGPIERAKEALARAWQYML"
     gene            complement(451..1065)
                     /locus_tag="VI195_10225"
     CDS             complement(451..1065)
                     /locus_tag="VI195_10225"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY6824806.1"
                     /translation="MKSSAQRAVQPIQFSLPPLPYAEDALAPVISAETLQLHHGKHHR
                     KYVDTMNQLLHEQPVAGNSLEDIVRASSGKLFNNAAQAWNHGFYWQSLSPKGGRPGAA
                     LLRQLEKDFGSFERFCEKFAAAAAAQFGSGWAWLVRKGGKLEVVTTSNADTPMKQGVQ
                     CLLTLDVWEHAYYVDYRNQRERYLSAVIENRLNWSFAEENLAKT"
     gene            complement(1087..1638)
                     /locus_tag="VI195_10230"
     CDS             complement(1087..1638)
                     /locus_tag="VI195_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824807.1"
                     /translation="MSRIVAGRFDRSIDADAALLNLQREGFGPGETSSFYVTPPGKHD
                     AHPLGGDSDSDAGSLHAGWGAILGAIAGACIGLVLGSLASAAYGPVAALIAAGLGAYV
                     GSFIGAMAKVRHGRKRDATLEHPAQAAGGRMVAVCVDRAGMEARAVAVLRRYGARDIG
                     CTQGDWRDGDWRDFDPRSPLAAV"
     gene            complement(1662..>2153)
                     /locus_tag="VI195_10235"
     CDS             complement(1662..>2153)
                     /locus_tag="VI195_10235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; sigma 54 factor is
                     responsible for the expression of enzymes involved in
                     nitrogen assimilation and metabolism; the rhizobia often
                     have 2 copies of this sigma factor; in Rhizobium etli
                     RpoN1 shown to be involved in the assimilation of several
                     nitrogen and carbon sources during free-living aerobic
                     growth and RpoN2 is involved in symbiotic nitrogen
                     fixation; in Bradyrhizobium both RpoN1 and N2 are
                     functional in free-living and symbiotic conditions, rpoN1
                     gene was regulated in response to oxygen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY6824808.1"
                     /translation="LQEARWLLRSIEQRFATIQRVADAIVARQRGFFEYGEVAMKPLT
                     LKLIAGELGLHESTVCRVTNNKYMATPRGLFEFKHFFSRRLATESGGAASATAVRAVM
                     KELIAAEDPRAPLSDAELARLLAQQGLRVARRTITKYRALMRMPSVDVRRADMRTGRM
                     ARA"
BASE COUNT          350 a          715 c          735 g          353 t
ORIGIN      
        1 cgtctggctc gcgataggct acttcgtgcc ggatcccgac acgctgcaga ttctcgagga
       61 agccgcccgc aggggcgtgg acgtgcgcct ggtgctgcct ggattcagcg acttctgggc
      121 gccgatcgca gccgcccggt ccaactacca gccgctactc gacgccggcg tgaagatcta
      181 cgagtggcgc caggcgctta tgcacgcaaa aaccgcggtc atcgacggac agtggtccag
      241 cgtcggctcg acgaacctcg actggcgcag cttcgtgcac aactacgagg atgatttgct
      301 ggtgttcgac cccgccttcg gccgcgagat ggaaaagcgc tatatggcgg atgtcggggc
      361 ttcgaccccg atcgacccta agacatgggc caatcggggg ccgatcgagc gcgccaagga
      421 agcgctcgcc agggcctggc agtacatgct ctaggtcttt gcgaggtttt cctcggcgaa
      481 cgaccagttg agccgattct cgatgaccgc agacagatag cgctcgcgct ggttgcggta
      541 gtcgacgtaa taggcgtgct cccatacgtc gagcgtgaga aggcattgca cgccctgctt
      601 catgggcgta tcggcgttgg acgtggtcac gacttcgagc ttgcccccct tcctgacgag
      661 ccaggcccat ccgctgccga actgcgcggc cgcggcggcg gcgaacttct cgcagaagcg
      721 ctcgaagctg ccgaaatcct tttcgagctg gcgcagcagc gccgcgccgg gcctgccgcc
      781 cttgggcgag agcgactgcc agtagaagcc atggttccag gcctgggccg cattgttgaa
      841 gagcttgccc gagctcgcgc gcacgatgtc ctccaggctg ttccccgcga ccggctgctc
      901 gtgcagcagc tggttcatgg tgtccacgta cttgcggtgg tgcttgccgt ggtggagttg
      961 cagcgtctcg gcggaaatga ccggcgccag cgcgtcctcg gcgtagggga ggggcggaag
     1021 ggaaaactga atgggctgca ctgcccgctg agcagaactt ttcatgaaac ctccaaaaaa
     1081 gggtggttaa accgcggcga gcggcgagcg cgggtcgaag tcgcgccagt cgccatcgcg
     1141 ccagtcgccc tgcgtgcacc cgatgtcgcg cgcgccatag cggcgcagca ccgccaccgc
     1201 gcgtgcttcc atgccggccc ggtccacgca cacggcgacc atccggccgc ccgccgcctg
     1261 ggccggatgc tcgagggtcg cgtcccgctt gcggccatgg cgcactttcg ccatcgcgcc
     1321 gatgaacgag ccgacatagg cgccgagccc ggcggcgatg agcgctgcca ccggaccgta
     1381 cgcggcggag gcgaggctgc cgagcacgag gccgatgcat gccccggcta tggcgcccag
     1441 gatcgcgccc cagcctgcat ggagcgagcc ggcgtcgcta tccgagtcgc cgccgagcgg
     1501 atgcgcatcg tgcttcccgg gcggcgtgac gtaaaaggag ctcgtctcgc cgggcccgaa
     1561 gccttcgcgc tgcaggttca agagcgcggc gtccgcatcg atggaacggt cgaatcttcc
     1621 ggcgacgatc ctggacataa agcctccttg gcctcgcgat atcaggcccg cgccatgcgg
     1681 cccgtgcgca tgtcggcacg gcgcacatcg acgctcggca tgcgcatcag cgcgcggtat
     1741 ttcgtgatgg ttcgcctcgc gacccgcagg ccctgctgcg cgagcaggcg ggcgagctcg
     1801 gcatccgaca gcggcgcgcg cggatcttcg gcagcgatga gctccttcat cactgcgcgc
     1861 acggcggtag ccgaggctgc gccgccgctt tccgtggcga ggcgccgcga gaagaagtgc
     1921 ttgaactcga agaggccgcg cggcgtagcc atgtacttgt tgttggtcac gcggcacacg
     1981 gtggactcgt gcagcccgag ctcgccggcg atcagcttga gggtgagggg cttcatcgcc
     2041 acctcgccgt actcgaagaa tccgcgctgg cgcgcgacga tggcgtcggc cacgcgctgg
     2101 atggtggcga agcgctgctc gatcgagcgc agcagccagc gcgcctcctg cag
//
LOCUS       DATDMP010000582         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1980807, whole genome shotgun sequence.
ACCESSION   DATDMP010000582 DATDMP010000000
VERSION     DATDMP010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980807"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VI195_10240"
     CDS             complement(<1..608)
                     /locus_tag="VI195_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HEY6824809.1"
                     /translation="MAAAETAGGATEYIVHHETFLSNRVPHGIVDFSTLNLDTVFFSV
                     LLAVVFAGGFYLAARRATTGVPGRFQLFVEVLVDFVDNQVKDTFHGTSQLIAPLALTI
                     FCWILLFNIMDLVPVDLLPAAAHEAGLSHLKVVPSTDLNATFAMSISVFILMIFYSIR
                     MKGFIGFVSELTLHPFNARNPFVQALLVPVNFLLESVTLLARP"
     gene            complement(633..1067)
                     /locus_tag="VI195_10245"
     CDS             complement(633..1067)
                     /locus_tag="VI195_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY6824810.1"
                     /translation="MEPVERDLYKVLAIDLPQARRLAFGVVLGQAGVTVIAALGGWAL
                     GGSGAAFSALLGGAIATAGSLAMAALVFAGAHRDAQRIVGAFYVGEAAKLAVVVGLFV
                     AVLRMVRVIPVALLGGFAATYLVYWIALVGALPSLGGTRRGA"
     gene            complement(1206..2078)
                     /locus_tag="VI195_10250"
     CDS             complement(1206..2078)
                     /locus_tag="VI195_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY6824811.1"
                     /translation="MKKPTLGRGLADLLGKAERPAPPAAGEELAKLPLDLLQRGRFQP
                     RVDMRDETLAELAGSIKAQGVVQPIVVRALGPPGAREPQRYEIIAGERRWRAAQMAGL
                     AEIPAVIRRIPDEAAIAIALIENIQRENLNPLEEARALTRLISEFGLTHQEAADAVGR
                     SRAAVSNLLRLLELAPEVCELIERRTLEMGHGRAVLALGSRRQQSEVALLVAKKGLSV
                     RETEALVRRLQTPGARPTEVSARDPNIERLEQELAEKLGARVAIQPAARGGGKLVVSY
                     HSLDELDGILAHIQ"
     gene            complement(2204..2307)
                     /locus_tag="VI195_10255"
                     /pseudo
     CDS             complement(2204..2307)
                     /locus_tag="VI195_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
BASE COUNT          357 a          841 c          782 g          327 t
ORIGIN      
        1 ggccgggcaa ggagcgtcac ggactcgagc aggaaattca ccggcaccag cagcgcctgc
       61 acgaacgggt tcctggcgtt gaaggggtgc agcgtgagct cactcacgaa gccgatgaag
      121 cccttcatcc tgatgctgta gaagatcatc aggatgaaga cgctgatcga catcgcgaag
      181 gtcgcgttga ggtcggtgct cgggaccacc ttcagatggc tcagccccgc ctcgtgggcc
      241 gcggccggca gcaggtcgac cggtaccagg tccatgatgt tgaacagcag gatccagcag
      301 aagatggtga gcgcgagcgg cgcgatcagc tggctggtgc cgtggaaggt gtccttcacc
      361 tggttgtcca cgaagtcgac cagcacctcg acgaacagct ggaagcggcc cggcaccccg
      421 gtggtggcgc ggcgggcagc gaggtagaag ccgcctgcga acaccaccgc gagcagcacc
      481 gagaagaaca ccgtgtcgag attgagcgtg gagaagtcga cgatgccgtg cggcacccgg
      541 ttcgacagga aggtctcgtg gtgcacgatg tactcggtcg cgccgccggc ggtctctgct
      601 gctgccatcg ccgttgccac tctcccgccg actcacgcgc cccggcgcgt gccacccaag
      661 ctcggcagcg cgcccaccag cgcgatccag tacacgaggt aggtcgcggc gaaaccgccg
      721 agcaacgcca ccggtatcac cctcaccatc ctcagcaccg ccacgaacag cccgaccacg
      781 acggcgagct tggcggcctc ccccacgtag aacgcgccca cgatgcgctg cgcgtcgcgg
      841 tgcgcgccgg caaacaccag cgccgccatc gccagactcc cggcggtggc gatcgcacca
      901 ccgagcagcg cggaaaacgc cgcgccgctt ccgccgagcg cccagccgcc gagcgccgcg
      961 atcacggtca cgcccgcctg acccagcacg acaccgaagg ccagccgccg cgcctggggc
     1021 agatcgatcg ccaacacctt atataagtcc cgctcgacgg gctccaagga gccgttgttc
     1081 ccgcgcctga gcgagggcgc ggggggcctg acttagctcg gccgttcagt cacttacgcg
     1141 atgacggcga aaaagccgcg ggattataga gaccccccga cgatcagtcc acagcgcact
     1201 tgtacctact ggatgtgcgc caggattccg tcgagttcat cgaggctgtg gtagctcacc
     1261 acgagcttgc cgccgccgcg cgcagccggc tggatcgcga cacgcgcacc gagtttctcg
     1321 gcgagctcct gctcgagccg ctcgatgttc gggtcgcgtg cgctcacttc cgtcggccgc
     1381 gcgcccggag tctggagccg gcgcaccagc gcctcggtct cgcgcaccga cagtcccttc
     1441 ttcgcgacca gcagcgccac ttcgctctgc tggcggcgac tgccgagcgc gagcaccgca
     1501 cggccgtgtc ccatctcgag cgtgcgccgc tcgatgagct cgcacacctc gggcgcgagc
     1561 tcgagcaggc gcagcaggtt gctcaccgcg gcacgcgagc ggccgaccgc atcggccgcc
     1621 tcctggtgcg tgagcccgaa ctcgctgatc aggcgcgtga gcgcgcgcgc ttcctcgagc
     1681 ggattcaggt tctcgcgctg gatgttctcg atgagtgcga tggcgatcgc cgcctcgtcg
     1741 gggatgcggc ggatgacggc cgggatctcg gcgagcccgg ccatctgcgc cgcccgccag
     1801 cgccgctcgc cggcgatgat ctcgtagcgc tgcggctcgc gggcgcccgg cgggccgagt
     1861 gcgcgcacca cgatcggttg caccacgccc tgcgccttga tcgagccggc gagctcggcg
     1921 agcgtctcgt cgcgcatgtc gacgcgcggc tggaagcgtc cgcgctgcag caggtcgagc
     1981 ggcagcttgg cgagctcctc ccccgcggcc ggcggggccg gccgctctgc ctttccgagc
     2041 aggtccgcaa gtccccggcc gagcgtcggc ttcttcatgc cggccggccc tcgccgagcg
     2101 cgctgcccgg ggcggtggcg cccgcctcgg ccgcggccgc cgccggtgcg gcagcgccga
     2161 gtccgccgtt accggcgccg gccgcgtggc cccccggggc cgcttcctcc tcgcgacgga
     2221 tcatctcgcc ggcgagcgcc aggtaggcga gcgcgccacg ggactccttg tcgtggaaca
     2281 gtgccggctt gccgaaggag ggtgcct
//
LOCUS       DATDMP010000583         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_92529, whole genome shotgun sequence.
ACCESSION   DATDMP010000583 DATDMP010000000
VERSION     DATDMP010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92529"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VI195_10260"
     CDS             complement(<1..873)
                     /locus_tag="VI195_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEY6824812.1"
                     /translation="MSNRLAHETSPYLQQHAANPVDWYPWGTEALERARAEAKPILLS
                     IGYSACHWCHVMAHESFEDPAVAALMNQLFVNIKVDREERPDLDQVYQTAHQMLAQRA
                     GGWPLTMFLTPQGAPFFGGTYFPTAARYGLPGFPDWCERIAAIWRERQADIAAQNAAL
                     EEAFSRLLPRSHAERAQLSPEVIDELVGELRAAFDARWGGFGGAPKFPHVPDLELCLR
                     HGAPDITQVTLTRMCEGGLFDQLGGGFYRYSVDAQWMIPHFEKMLDDNGPLLGLLADG
                     WLVTREPLYRSVATE"
     gene            complement(927..2336)
                     /locus_tag="VI195_10265"
     CDS             complement(927..2336)
                     /locus_tag="VI195_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824813.1"
                     /translation="MKALLPRSLFGRTLLVLAVGLLLAQLASQAVNLFDRGSSVYRLA
                     TFQLASRIAQAARILNRLPAHRRSAVVQEIDDPHLAVSVSARPLPVPGYQQLDRYEKA
                     FAESLRRQLERDWPVSVAITPEPRSRRAPGEGTVASPFEIWIAHHFYFLLPGTFSLVA
                     QVGLEDGSTAVFYARIPQEPLSRLESLLPRLALTLTIFLLLAAIMVRMMTRSLDRLGR
                     AAAQLGADVEGAPLPESGPREVRSVIGVFNRMQGQITRHVQERTRLLGAISHDLKTPL
                     ARMRLRTELLPDAGLRARFVRDLDEMDSMVRATLEFLGSLGESPKLVPVDVGALMESL
                     CEDYLESGADVTLAGEAPAPYRAHPQALRRCVGNLIDNALRYGGCAEVALQNGEGALR
                     VTVRDHGPGIPEGELERVFDPYFRLDASRNRDSGGTGLGLSIARNIARWHGGEIRLRN
                     AIDTSGLIAELTLPQPAAQ"
     gene            complement(2333..>2742)
                     /locus_tag="VI195_10270"
     CDS             complement(2333..>2742)
                     /locus_tag="VI195_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY6824814.1"
                     /translation="KPFNPRELLARLKALLRRSRALGNPRPEMRRVRFAGWTLDLAAR
                     HLVDPGGVVVSLSGGEFRLLAVFVEHANRVLDRNLLMDLALGREATPFDRSIDVQVSR
                     LRQRMRDDGREPRIIKTVRNSGYVLAAAVERLP"
BASE COUNT          442 a          937 c          965 g          398 t
ORIGIN      
        1 ctcggtggca acgctccggt agagcggctc gcgcgtgacc agccacccgt cggccagcag
       61 gccgagcaac gggccgttgt cgtcgagcat cttctcgaag tgcggaatca tccactgcgc
      121 gtcgacgctg tagcgataga agccgccccc gagctggtcg aacagcccgc cctcgcacat
      181 gcgcgtcagc gtcacctgcg tgatatcggg cgcgccgtgg cgcaggcaca gctcgaggtc
      241 gggcacgtgc ggaaacttcg gcgcgccgcc gaagccgccc caccgcgcat cgaatgccgc
      301 gcgcagctcg cccaccagct cgtcgatgac ctcgggcgaa agctgtgcac gctccgcgtg
      361 gctgcgcggc aggagccggc tgaaggcttc ctcgagcgcg gcgttctgcg cagcgatatc
      421 ggcctgtcgc tcgcgccaga tcgcggcaat ccgctcgcac cagtccggaa atcccggcag
      481 tccgtagcgg gccgccgtgg gaaagtaggt gccgccgaaa aagggcgcgc cctgcggcgt
      541 gagaaacatc gtgagcggcc agccgccggc gcgctgcgcg agcatctggt gcgccgtctg
      601 gtagacctgg tcgaggtcgg gtcgctcttc gcgatcgacc ttgatgttga cgaacagttg
      661 attcatcaat gcggcgactg ccggatcttc gaacgattcg tgcgccatca catggcacca
      721 atggcacgcc gaatagccga tcgagagcag gatcggcttc gcttcggcgc gtgcccgctc
      781 gagcgcctcg gttccccacg gataccagtc gaccggattg gccgcatgct gctgcaaata
      841 gggagaggtc tcgtgcgcga ggcgattgga catggcgagg gcgaccgcaa accggcggga
      901 ttggcgacag accgcctagc ttagcgctac tgcgcggcgg gttgcggcaa cgtgagctcg
      961 gcgatcagcc cggacgtatc gatggcgttg cgcaggcgga tttcgccgcc gtgccagcgg
     1021 gcgatgttgc gcgcgatgct caagccgagc ccggtgcccc cgctgtcccg gttgcgcgaa
     1081 gcgtcgagcc ggaagtaggg gtcgaagacg cgctcgagct cgccctccgg gatgcccggg
     1141 ccgtggtccc gcacggtgac acgcagcgcg ccctcgccgt tctgcagcgc gacctccgcg
     1201 cagcccccgt agcgcagggc attgtcgatg aggttgccca cgcagcgacg cagcgcctgc
     1261 ggatgggcgc ggtacggcgc gggcgcctcc ccggcgagcg tcacgtcggc gccgctctcg
     1321 aggtaatctt cgcagaggct ttccatcagc gcgccgacgt ccaccggcac gagcttgggg
     1381 ctttcgccga ggctgccgag gaattccagc gtcgcgcgga ccatggaatc catctcgtcg
     1441 aggtcgcgca cgaacctggc gcgcagcccg gcgtccggca gcagctcggt gcgcagccgc
     1501 atgcgtgcga gcggcgtctt gaggtcgtgc gagatcgcgc cgagcagccg agtgcgctcc
     1561 tgcacgtgcc tcgtgatttg cccctgcatg cggttgaaga cgccgatcac gctgcgcacc
     1621 tcccgcggac cgctctccgg aagcggcgcg ccttcgacgt cggcgccgag ctgcgccgcg
     1681 gcgcgcccga gtcggtcgag cgagcgcgtc atcatgcgca ccatgatcgc cgcgagcagg
     1741 aggaagatgg tcagcgtgag ggcgagccgc ggcagcagcg actctaggcg gctcagcggt
     1801 tcctgcggga tgcgggcata gaacaccgcg gtgctgccgt cctccaggcc tacctgggca
     1861 accagcgaga aggtgccggg gagcaggaaa tagaagtggt gcgcgatcca gatctcgaac
     1921 gggctcgcca ccgtgccttc gccgggcgcc cggcgcgagc gcggttcggg cgtgatcgcc
     1981 acgctcaccg gccaatcgcg ttcgagctgc cggcgcagcg actcggcaaa ggccttctcg
     2041 tagcgatcga gctgctggta gcccggcacg ggaagcgggc gcgcgctcac gcttacggcg
     2101 agatgcgggt cgtcgatttc ctggactacc gcgctgcgcc gatgcgccgg caggcggttc
     2161 agaatgcgcg ccgcctgcgc aatacgcgac gcgagctgga atgtcgcgag gcggtagacc
     2221 gagctgccgc gatcgaacag gttgaccgcc tggctggcga gctgcgcgag cagcaatccc
     2281 acagcaagca ctagcaaggt gcgaccgaac agcgagcgcg gcaagagcgc cttcacggca
     2341 gccgctccac ggcggcggcg agcacatacc cgctgttgcg caccgtcttg atgatgcgcg
     2401 gctcgcggcc gtcgtcgcgc atgcgctggc gcaggcggct cacctgcacg tcgatgctgc
     2461 ggtcgaaggg cgtcgcctcg cgcccgaggg cgaggtccat cagcaggttg cggtcgagca
     2521 cccggttcgc atgctcgacg aatacagcca gcagcctgaa ctcgccgccg ctcaacgaaa
     2581 cgaccacgcc gccgggatcg accaggtggc gcgccgcgag atcgagcgtc cagccggcga
     2641 aacgcacgcg ccgcatttcc ggccgcgggt tgccaagcgc acgcgagcgg cgcagcagtg
     2701 ccttcagccg cgcgagcagc tcacgcggat tgaacggctt gc
//
LOCUS       DATDMP010000584         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1758955, whole genome shotgun sequence.
ACCESSION   DATDMP010000584 DATDMP010000000
VERSION     DATDMP010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758955"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VI195_10275"
     CDS             <1..902
                     /locus_tag="VI195_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6824815.1"
                     /translation="EPNLGYIAEHRVLQRALLAAFAEAGGHIEPAAFSSLAIGDAEVE
                     VSTPQRVLRARLVIGADGAQSAVRAAAGLTVDTRAYGQTAIVANVTTESAHESTAWQR
                     FMRDGTLAFLPLPDGTSSIVWSADDSRAASLVSLSDAAFAAELDRASDFALGRTQLRG
                     ERLSFPLTRLAAQRYVAQRVALIGDAAHVVHPLAGQGMNLGLLDAAALAQQVKAALAA
                     REDPGALRVLRAYERWRKSEAAVMAISIDTFDRLLAHGVGPMARLAQRGLSWVNASQE
                     LRRFFIRRALGLSGELPEAARLV"
     gene            1005..1361
                     /locus_tag="VI195_10280"
     CDS             1005..1361
                     /locus_tag="VI195_10280"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY6824816.1"
                     /translation="MLNYVALDKVFKSLADPWRRHMIECLCDGEASVSWLAEHLPLSL
                     SAILKHLQVLENNGLIHTEKVGRQRICRIEPQALQLLHQWVSPRRKVWDHRLRRLLAA
                     ANYRLYQLDDPDAGSR"
     gene            complement(1353..1691)
                     /locus_tag="VI195_10285"
     CDS             complement(1353..1691)
                     /locus_tag="VI195_10285"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY6824817.1"
                     /translation="MSDLAARRCTPCEGGIPPLTPAEAQRLLAQISSAWALTENTRAL
                     RREFKFRDFYRTMSFVNALAHIANIEDHHPDLEVGYNYCRVRYTTHAIGGLSENDFVC
                     AAKIDLIPLA"
     gene            complement(1688..>2608)
                     /gene="ispG"
                     /locus_tag="VI195_10290"
     CDS             complement(1688..>2608)
                     /gene="ispG"
                     /locus_tag="VI195_10290"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEY6824818.1"
                     /translation="AALAKYRINPGNVGRGSKRDPQFAEMIEVACRHEKPVRIGVNWG
                     SLDSDLLTRLMDENSARAEPLDARQVMRSAVVLSALGSAQRAEQLGLPHDRIILSAKV
                     SGVQDLIAIYRDLAGRCDYPLHLGLTEAGMGSKGIVASTAGMAVLLQEGIGDTIRVSL
                     TPEPNGDRTQEVIVAQEILQTMGLRSFTPLVVACPGCGRTTSTFFQELAARIQSHIRT
                     RMPQWRREYQGVEDMTVAVMGCVVNGPGESKHANVGISLPGTGERPVAPVYEDGAKTV
                     TLKGERIAEEFQEIVEQYVARHYARRAADA"
BASE COUNT          400 a          867 c          872 g          469 t
ORIGIN      
        1 ctgagccgaa cctcggctat atcgccgagc atcgcgtcct gcagagggcg ctgctcgctg
       61 cattcgccga ggccggcggt cacatcgagc ccgcggcgtt cagctcactc gcgatcgggg
      121 acgcggaggt cgaggtgagc acgccacagc gtgtgctcag ggcgcgactg gtcatcggcg
      181 ccgatggcgc gcaatcggcg gtgcgtgccg cggcgggcct cacggtagac acccgtgcct
      241 acgggcagac ggccatcgtc gccaacgtga caaccgagag cgcgcacgag tccactgcct
      301 ggcagcgctt catgcgcgat ggcacgctcg cattcctgcc tctccccgat ggcacgagct
      361 cgatcgtctg gtcggcggac gattcacggg ccgccagtct cgtgagcctg agcgatgcgg
      421 ccttcgcggc cgagctagat cgcgcgtccg atttcgccct cggccggacg cagctgcgcg
      481 gcgaacgcct gtcgtttccg ctgacgcgac tggcggcgca gcgctacgtg gcgcagcgcg
      541 tcgcactgat cggcgatgcc gcgcatgtcg tccatccgct tgcgggtcag gggatgaacc
      601 tcggcttgct cgatgccgcc gcactcgccc aacaggtcaa ggccgccctg gcggcgcgcg
      661 aggacccggg cgcgctgcgc gtgctgcgcg cctacgagcg ctggcgcaag agcgaggccg
      721 ccgtcatggc gatctcgatc gatactttcg accggctgct cgcacacggt gtgggcccga
      781 tggcgcgcct ggcgcagcgg ggactctcct gggtcaatgc cagtcaggag ctgcggcgat
      841 ttttcatccg acgcgccctc ggcctcagcg gcgagctgcc ggaggcggcg cgcttggttt
      901 gagggctgac tttttaatgt gtttgttcga atagttcacc atttggtgaa ctattgcgga
      961 aacgtgtgtt gatcgcgcgc ccggcactcc tagactcggc ccacatgctc aactacgtgg
     1021 cgctcgataa ggtgttcaaa tctctcgccg acccgtggcg gagacacatg atcgagtgtc
     1081 tctgcgatgg ggaggcatcc gtgagctggc tggcagagca cctgccgctg agcctgtctg
     1141 cgattctcaa gcacctgcag gtgctcgaga acaacggtct gattcacacg gagaaggttg
     1201 gccgccagcg catctgtcga atcgagccgc aggcactgca gctgctccat cagtgggtca
     1261 gcccgcgacg caaagtctgg gaccaccgtc tgcggcggct gctcgccgcg gccaattacc
     1321 ggctttacca gctcgacgat ccagacgccg gctcacgcta gcggtatcag atcgattttg
     1381 gccgcgcaga cgaagtcgtt ttcggaaagc ccgccgatgg cgtgcgtcgt gtagcgcacg
     1441 cggcagtagt tgtagccgac ctcgaggtcc ggatgatggt cctcgatgtt cgctatgtgc
     1501 gcgagtgcgt tgacgaagct catggtgcgg tagaagtcgc ggaacttgaa ctcgcgccgc
     1561 aaggcacgag tattttctgt cagcgcccag gcgctggaga tctgcgcaag cagccgctgc
     1621 gcctcggccg gggtgagcgg tgggatgcct ccttcgcagg gcgtgcagcg ccgtgccgcg
     1681 agatcgctca tgcgtcagcc gcccggcgcg catagtgccg cgccacgtac tgctcgacga
     1741 tctcctggaa ttcctcggca atgcgctcgc ctttcagggt caccgtcttg gcgccatcct
     1801 cgtacaccgg tgcgaccggg cgctcgccgg tgcctggcag gctgatgccg acgttggcgt
     1861 gcttgctctc gcccggtcca ttcacgacgc accccatcac cgctaccgtc atgtcctcga
     1921 cgccctggta ttcgcggcgc cactgcggca tgcgcgtgcg gatgtgcgac tggatgcgcg
     1981 cggcaagctc ctggaagaag gtgctggtcg tgcgcccgca ccccgggcag gcgactacga
     2041 gcggggtgaa ggatcgcaga cccatggtct gcaggatctc ctgcgccacg atgacctcct
     2101 gcgtgcggtc accgttgggc tccggcgtga gggagacgcg aatggtgtca ccgatccctt
     2161 cctgcagcag caccgccatg ccggcggtgg atgcgacgat gcctttcgat cccatcccgg
     2221 cctcggtgag accgaggtgc agcggatagt cgcaacggcc ggcgagatcg cggtagatcg
     2281 cgatcaggtc ctgcacaccg ctcaccttgg cggagaggat gatgcgatcg tgcggcaggc
     2341 cgagctgctc ggcgcgctgc gcactcccca gcgccgagag caccaccgcg ctgcgcatca
     2401 cctgccgtgc atccaacggc tcggcgcgcg cagagttctc gtccatgagc cgcgtcagca
     2461 ggtctgagtc gaggctgccc cagttcacac cgatgcgcac cggcttctcg tggcggcacg
     2521 ccacctcgat catctcggcg aactgcggat cgcgcttgct gccacgtccg acgttgccgg
     2581 ggttgatgcg gtacttcgcg agcgccgc
//
LOCUS       DATDMP010000585         3440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1314805, whole genome shotgun sequence.
ACCESSION   DATDMP010000585 DATDMP010000000
VERSION     DATDMP010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314805"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1446
                     /gene="ffh"
                     /locus_tag="VI195_10295"
     CDS             82..1446
                     /gene="ffh"
                     /locus_tag="VI195_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY6824819.1"
                     /translation="MFDNLTARLSRTIENLRGRGRITEENIAETLRETRVALLEADVA
                     LPVVKSFIESVKAKALGAEVLSSLSPGQAFIGILYQELVQLMGGAGGGFAPRAQPPVV
                     LLLAGLQGAGKTTTAAKLARRLIEREKKRVLLASTDVRRPAAMLQLERLAGQVQAEYF
                     PAPPAAAPAQIARAALERARSGVFDMLIVDTAGRLHVDEELMAEVRQIDAAVGAAERL
                     FIVDAMAGQDAVNAARAFGAALDLTGVILTKADGDARGGAALSVRQITGKPIVFLGVG
                     EKTEALEPFDPERMASRILGMGDVVSLVEQVHAKVDQAEAQRIAHKVVKGKGFDMTDL
                     RSQLEQLQKMGGVGALLDKLPGAVNRKGAVSADQGDRELRRQIAIINSMTPRERRSPG
                     IIDGSRRRRIAGGSGVQVQDVNRLLKQFQEMQRVMKSMKGGRLRALMGALKGGGLPPG
                     FPGR"
     gene            1564..1818
                     /gene="rpsP"
                     /locus_tag="VI195_10300"
     CDS             1564..1818
                     /gene="rpsP"
                     /locus_tag="VI195_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY6824820.1"
                     /translation="MVTIRLTRRGARNQPFYHVVVTDSRKRQGGPSLEHVGFFNPIAR
                     RGEQKLKLDLPRIDYWVGKGAQPSDRVRSLVASFRKQATA"
     gene            1864..2370
                     /gene="rimM"
                     /locus_tag="VI195_10305"
     CDS             1864..2370
                     /gene="rimM"
                     /locus_tag="VI195_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY6824821.1"
                     /translation="MEWIELGRVGAPFGIKGWVHIESHTAPPEGLLGYNEWTLRLASG
                     ERVVRRIIEGRRHATGLVAHLEGVEDRGAAAALGGVVIEIERARLPRLPEREYYRADL
                     QGLRVRNLEGAELGTVSHFIEAPSGAVMVTREAGGREHWVLAAPPYLRRVDLTQGEVL
                     VDWPAELE"
     gene            2378..3127
                     /gene="trmD"
                     /locus_tag="VI195_10310"
     CDS             2378..3127
                     /gene="trmD"
                     /locus_tag="VI195_10310"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY6824822.1"
                     /translation="MKIEVVTLFPRMIAGALEFGVVGRAIARGLLSVGTEDPRTHTSD
                     VHRTVDDRPYGGGPGMVMKPEPLMAAIRAGHARLPQGSPRVYLSAQGEPFGQAQAQQL
                     AQLPGLLLIAGRYEGIDERVIGLGSDRELSVGDYVLSGGELPALIVIDALARLLPGVL
                     GDERSSVEESFAAGLLDWPHYTRPELFEGAAVPAVLLSGDHADIQRWRLQQALARTAE
                     RRPDLLRKAQLSDEAQRLLHEIRARDRGERT"
     gene            3124..>3440
                     /gene="rplS"
                     /locus_tag="VI195_10315"
     CDS             3124..>3440
                     /gene="rplS"
                     /locus_tag="VI195_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY6824823.1"
                     /translation="MSKIIEEIDKQRMTREVPDFKPGDTVVVQVKVVEGDRERVQAYE
                     GVVIAKSNRGLNSSFTVRKISHGEGVERVFQTHSPAISDITVKRRGSVRRAKLYYLRD
                     LSG"
BASE COUNT          545 a         1083 c         1270 g          542 t
ORIGIN      
        1 gcatagaagc tacgggcgcg gcgggtaaat gcggccctgc cccctataat cgcccgctcg
       61 gctcacggat cacggcttga catgttcgat aacctgaccg cccggctgtc gcgcaccatc
      121 gagaacctgc gggggcgcgg ccgcatcacc gaagagaaca tcgccgagac actccgcgag
      181 acgcgcgtgg cgctgctcga ggcggacgtg gccctgcccg tggtgaagtc gttcatcgag
      241 tcggtcaagg ccaaggcact cggcgccgag gtgctctcga gcttgagccc cggacaggcg
      301 ttcatcggca tcctctacca ggagctggtg cagctgatgg ggggtgccgg tgggggcttc
      361 gcgccgcgtg cacagcctcc ggtcgtgctg ctgctcgcgg gcttgcaggg ggcgggcaag
      421 acgaccaccg ccgcgaagct cgcccggcgg ctcatcgagc gcgagaagaa gcgcgtgctg
      481 ctcgcgagca ccgacgtgcg gcggccggcg gcgatgctgc agctcgagcg gctcgccggg
      541 caggtgcaag ccgagtattt ccccgcgcca ccggccgcgg cgcccgcgca gatcgcccgc
      601 gccgcgctcg agcgggcccg cagcggggtg ttcgacatgc tgatcgtcga caccgccggc
      661 cggctgcatg tcgatgagga gctcatggca gaggtgcgcc agatcgatgc cgccgtcgga
      721 gctgcggagc ggctcttcat cgtcgatgcc atggcgggcc aggacgcggt gaacgccgcg
      781 cgcgccttcg gcgccgcgct cgatctcacc ggcgtcatcc tcaccaaggc cgacggcgac
      841 gctcgtggtg gtgcggcgct ctcggtgcgg cagattaccg gtaagccgat cgtgttcctc
      901 ggggtcggcg agaagaccga ggcgctcgag ccgttcgatc ccgagcgcat ggcctcgcgc
      961 attctcggca tgggtgatgt cgtgagcctc gtcgagcagg tgcacgccaa ggtcgatcaa
     1021 gccgaggcgc agcggatcgc gcacaaggtc gtcaaaggca agggcttcga catgaccgat
     1081 ctcaggagcc agctcgagca gctgcagaag atgggcggtg tcggggcgct gctggacaag
     1141 cttccggggg cggtgaacag gaagggggcc gtttcggcgg accagggcga tcgggagctg
     1201 cgccggcaga tcgccatcat caactccatg accccgcgcg agcggcgcag ccccgggatc
     1261 atcgatggct cgcgccggcg gcgcatcgcc ggcggctcgg gagtgcaggt tcaggacgtc
     1321 aatcgcctgc tcaagcagtt ccaggagatg cagcgggtca tgaagagcat gaagggtgga
     1381 cggctgcggg cgctcatggg ggccctcaag ggcggcggcc tgccgccagg gttccccggc
     1441 cgctgaccgg cttccccgga gcggtggaaa aactggcgtc ggattcgcac gttgcgtaga
     1501 atccgcgctc ttttttgtgc ggcctttctg cagggcccgc gctggagcga gagcagtacg
     1561 accatggtaa ccattcgcct gacgcggcgc ggggcgcgta atcagccctt ctatcacgtc
     1621 gtcgtcaccg acagccgtaa gcgccagggc ggcccgagcc tcgagcacgt gggtttcttc
     1681 aacccgatcg cccgtcgcgg ggagcagaag ctcaagctcg atctgccgcg tatcgactac
     1741 tgggtcggca agggggctca gccctccgat cgggtgcgca gcctcgtggc ttcctttcgg
     1801 aagcaggcta ccgcctgaag cgcgccgccg ctgcgtagcg gcgctgcggg cctgtcgacg
     1861 gccgtggaat ggatcgagct cggtcgcgtc ggcgctccct ttggcatcaa gggctgggtg
     1921 cacatcgagt cgcataccgc gccgccggag gggttgctcg gttacaacga gtggacacta
     1981 cggctcgcga gcggcgagcg ggtcgtgaga cggatcatcg agggacgccg gcatgcaacg
     2041 ggtttggtgg cgcatctcga gggagtggag gatcgcggcg cagccgccgc gctcggcgga
     2101 gtcgtgatcg agatcgagcg tgcgcgcttg ccgcgcctgc ccgagcggga gtactaccgc
     2161 gcagatctcc agggcctgcg cgttcgtaac cttgaaggtg cggagctcgg cacggtgagc
     2221 catttcatcg aggccccctc gggcgcggtg atggtgacgc gtgaggcggg gggacgcgag
     2281 cactgggtgc tggcggcgcc gccgtacctg cgccgcgtgg acctcactca gggcgaggtt
     2341 ctggtggatt ggccggcgga gctggaatag aggctccatg aagatcgagg tggtcacgct
     2401 gtttccgcgc atgatcgcgg gagcactcga gttcggtgtc gtggggcgcg cgatcgcccg
     2461 cgggctgttg agcgtcggca ccgaggatcc gcgcacgcac acgagcgatg tgcaccgcac
     2521 ggtcgacgat cggccctatg gcggcggtcc ggggatggtg atgaagcccg agccgctgat
     2581 ggccgcgatc cgcgccgggc atgcgcggct cccgcagggg agtccgcggg tgtacctgtc
     2641 ggcacagggc gagcccttcg ggcaggcaca ggcgcagcag ctcgcgcagt tgccggggct
     2701 gttgctgatt gccggtcggt acgagggtat cgatgagcgc gtgatcgggc tcggcagcga
     2761 tcgcgagctg tcggtcggtg actacgtgct ctccggtgga gagttgcccg cgctcatcgt
     2821 aatcgatgcg ctggcgcggc tcctgcccgg agtgttgggc gatgagcgtt cgagtgtcga
     2881 ggagtccttc gccgcggggc tgctcgactg gccgcactac acgcggccgg aattattcga
     2941 gggcgcggcg gtgccggcgg tactattgtc gggcgatcac gccgacattc aacgctggcg
     3001 tctgcagcag gcgctggcgc gcaccgcgga gcgccggccg gacctgctgc gcaaggcgca
     3061 gctctcggac gaggcgcagc gcctgttgca cgagataagg gcccgcgatc gaggtgagcg
     3121 aacatgagca agatcattga ggaaatcgac aagcagcgca tgacccgcga ggtcccggac
     3181 ttcaaacccg gcgacaccgt ggtggtgcag gtgaaggtgg tggagggcga tcgcgaacgc
     3241 gtgcaggcct acgagggagt cgtcattgcc aagagcaatc gcgggctgaa ctcatccttc
     3301 accgtgcgca agatctccca tggcgaaggc gtcgagcgcg tcttccagac ccacagcccc
     3361 gcgatcagcg acatcaccgt caagcgccgc ggcagcgtgc gccgcgccaa gctctattac
     3421 ctgcgcgatc tctcgggcaa
//
LOCUS       DATDMP010000586         3660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2092886, whole genome shotgun sequence.
ACCESSION   DATDMP010000586 DATDMP010000000
VERSION     DATDMP010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092886"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VI195_10320"
     CDS             complement(<1..338)
                     /locus_tag="VI195_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824824.1"
                     /translation="MASKTVWIAGGVVVAIGVVAYLSFNGSPAGKDGAGTIVEAKRIH
                     ADAPPSTTSTSTTSTSDQSSAATEAGNPGASNPADASAAKSGADAAALSAEQKAALSA
                     EQKAALSAEQ"
     gene            complement(434..1117)
                     /locus_tag="VI195_10325"
     CDS             complement(434..1117)
                     /locus_tag="VI195_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824825.1"
                     /translation="MVDSRQLALIHADMDGELDGRERAELARCLLADSEARALHEELR
                     HLCSALDAMPKAEPPPQLAAKILAALPHSTRRLAAPSWSQPRWRYAAVIAAALVAATV
                     LFETVKVSEPVTSDGAGTMAAPGTPAIVNTVRLDHGPVSGQVSLYRDHAGLGLALDLV
                     ADSPIDVLIASNGQTLRVRGLRRAAAAGPPTMVPLPGVAMAGQAINVTFLVNSQEVGQ
                     ATFRAPKEP"
     gene            complement(1119..1658)
                     /locus_tag="VI195_10330"
     CDS             complement(1119..1658)
                     /locus_tag="VI195_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6824826.1"
                     /translation="MNDEDDRRLVRRCRGGERAAFEQLVVRYQKPVFNAALRLLRDPE
                     DASDVAQTTFLKAFEHLGKYNPDFKFYSWLYRIAINEALDVLNSRTPADGISDEEPDG
                     QPGPERRAEGEQMGRAIEDALMRIKPDLRTVIVLRHFMHLSYEDMGEILMLPEKTVKS
                     RLHSARQLMRDQLFQYAVS"
     gene            1880..3100
                     /locus_tag="VI195_10335"
     CDS             1880..3100
                     /locus_tag="VI195_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY6824827.1"
                     /translation="MKGNALTGAAGTVTGTVRGLADSLLGSRGRYAPAFENLILALIV
                     FSVASIGIDTLPGLPAWAGSALRAAEVAVVAVFTFEYLLRIVAAGEKLAFIASFQGVV
                     DLLAIAPFFLFGFDARWVRALRLLRLLRVLKLQTHFLEATVAERTGALVAKNAALEQA
                     QAQLKAELEVARALQLAILPSAFPVRAGCDGAARMIPATTMGGDFYDFIELPAGQVGL
                     VTADVSGHGVPAAFFMAVARTYLRDLALQHETPGGCLAHANEALCTQNPLALFVTVFY
                     CMLDPATGLLRYANGGHNPPYVRRNDGAVEALNGAAGPALGVLPGVGFPDHRLQLRPG
                     DRLVLYTDGVTEAFNPANEAYGAERLIAEVRAAGDGAAAVLVERICRSITTFSAGAPQ
                     ADDITLTVLAWRAP"
     gene            3097..3444
                     /locus_tag="VI195_10340"
     CDS             3097..3444
                     /locus_tag="VI195_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6824828.1"
                     /translation="MIVRWETLAQATVVIVEGRLDFGASPGFQQQLEQALAGAGTPPA
                     AVIIDCAALDYVSSAGLRVFLLAARESQRLGIGFALCALRPAVREVFDLSGFTRVIAV
                     HPDRPTALEPPSA"
     gene            3441..>3660
                     /locus_tag="VI195_10345"
     CDS             3441..>3660
                     /locus_tag="VI195_10345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824829.1"
                     /translation="MKDRHTKVASDAAQLPLLMQFLREFWSAAGLPPAQATAFELALE
                     EVFMNVVRHGSPQGTAPWVEVSLALTADG"
BASE COUNT          553 a         1266 c         1213 g          628 t
ORIGIN      
        1 ttctgctcag ccgacagcgc agccttctgc tcagccgaca gggcagcttt ctgctcagcc
       61 gacagcgcag ctgcatccgc accagacttc gcagccgacg catcagccgg gttggaggcg
      121 ccaggattcc cggcctccgt cgccgcggag ctctgatcgg acgtactcgt cgtcgacgtg
      181 ctcgtcgtcg atggcggggc atccgcgtgg atgcgcttcg cctcaacgat agtcccagca
      241 ccatccttgc ccgcgggcga tccgttgaag ctcaggtagg cgaccacgcc gatcgcgact
      301 acaacgccgc ctgcaatcca taccgtcttg ctagccataa gaaaacctcc ccagaacctc
      361 tcgagtgatt tgcgctctgc aataattacc gtgggatctc aaaaaagttg gttcattgtc
      421 ggagaacgcc gtttcagggc tccttcggcg cccggaacgt cgcttgcccg acctcctggc
      481 tgttcaccag gaaagtcacg tttatcgctt ggcccgccat cgcgaccccg ggcaggggaa
      541 ccatcgtcgg tggccctgcg gcggccgctc tccgaagacc tctgacccgc aacgtttgtc
      601 cattgctcgc gatgaggaca tcgatggggc tgtccgccac cagatcgagt gccaagccga
      661 gcccggcgtg gtcgcggtaa aggctcacct ggcccgaaac gggcccgtgg tcgagacgca
      721 cggtgttcac gatcgccgga gtgccgggcg ccgccatcgt acccgccccg tcgctggtga
      781 caggttccga aaccttcacg gtctcaaaca gcacggttgc cgcgaccagg gcagctgcaa
      841 tcacggcggc ataacgccag cgaggctgcg accacgaagg cgccgcaagc ctcctcgtcg
      901 aatgtggcag cgcagcaagg atcttcgccg cgagctgcgg cggtggctcg gccttcggca
      961 ttgcatcgag tgccgagcac agatgccgga gctcctcgtg cagcgcgcgc gcctccgagt
     1021 cggcaagcag gcagcgcgcc agctcggcgc gctcgcggcc gtcgagctcg ccgtccatgt
     1081 cggcgtggat gagggcgagc tgccgggagt ccaccatgtc acgacaccgc gtactggaac
     1141 agctggtccc gcataagctg gcgggcgctg tgaaggcgcg atttcacggt cttctcgggg
     1201 agcatgagga tctcgcccat gtcttcgtaa ctcagatgca tgaagtgacg cagcacgatc
     1261 acggtccgca agtcgggctt gatccgcatc agcgcatcct cgatcgcccg tcccatctgc
     1321 tcgccctcgg cccggcgctc ggggccgggc tgcccgtccg gctcctcgtc actgatcccg
     1381 tcggcgggcg tgcgcgaatt gagaacatcg agggcttcat tgatcgctat ccggtaaagc
     1441 cagctgtaga acttgaagtc cgggttgtat ttgcccaggt gctcgaacgc cttgaggaac
     1501 gtggtctgcg cgacgtcgct ggcatcctcc ggatcgcgga gcagccgcag cgccgcattg
     1561 aagaccggct tctgataccg caccacgagt tgctcgaaag ccgcccgctc gcctccgcgg
     1621 caacgccgca ccagccttct atcgtcctcg tcattcacaa gtactgctcg gcatggaaaa
     1681 ggttggccgg gccaggctgg ctcgatgcgt gcatcggctg tcttgagggc agccgttctc
     1741 tccgcgccgc gcggattccc cccgaaccgc aggctacgcg ctttcaccgg acattgcatc
     1801 ggcgcgggtg ttggaacggg taaggtccgg gctgccgcgg ttgcgcaggc tgattgctta
     1861 cccgaatatc gagcagacaa tgaaaggcaa cgcgctcacg ggggcagccg ggaccgtgac
     1921 ggggacggtc cggggcctgg ccgactcgct cctcgggtcc cgggggcgct acgcccctgc
     1981 ctttgagaac ctgatcctgg cgctgatcgt cttcagcgtt gcctccatcg gtatcgacac
     2041 actgcccggc ctgcccgcct gggccggaag cgcgctgcgc gccgccgagg tcgcggtggt
     2101 ggcggtgttc accttcgaat atctgctgcg catcgtcgcg gctggggaaa aactcgcatt
     2161 catcgcgagc ttccagggcg tggtggacct gctggcgatc gcgcccttct ttctcttcgg
     2221 gttcgacgcc cgctgggtgc gggcgctgcg cctgctgcgg ctgctgcgcg tgctgaaact
     2281 gcagacccac tttctcgagg cgacggtggc cgagcgcacc ggggcgctgg tcgcaaagaa
     2341 tgccgcgctc gagcaggcgc aggcgcagct caaagcagag ctcgaagtcg cccgggcgct
     2401 gcagctggcg atcctgccgt cggccttccc ggtacgcgca ggctgcgacg gcgcggcgcg
     2461 catgatcccg gcgactacca tgggcgggga cttctacgac ttcatcgagc tccccgccgg
     2521 gcaggtcggg ctcgtgaccg ccgacgtctc cggccacggc gtcccggcgg cattcttcat
     2581 ggccgtcgcg cgcacctacc tgcgcgatct ggcgctgcag cacgagaccc cgggcggctg
     2641 tcttgcgcac gccaacgagg cgttgtgcac acagaatccg ctcgcgctct tcgtgaccgt
     2701 tttctactgc atgctggatc ccgcgaccgg gctgctccgc tacgccaacg gcggccacaa
     2761 cccgccgtac gtacggcgca atgatggggc ggtcgaggcg ctgaacgggg ctgccggccc
     2821 tgcgctcggt gtgctgcccg gtgtcgggtt tcccgatcac cggctgcagc tgcggcccgg
     2881 tgaccggctg gtgctctaca ccgatggcgt gaccgaggcc ttcaaccccg ccaacgaggc
     2941 ctacggcgcc gagcggctga tcgccgaggt ccgggcagcc ggcgacggcg cggcggcggt
     3001 gctggtcgag cgcatctgcc gcagcatcac caccttcagc gccggcgccc cgcaagccga
     3061 cgacatcacg ctaaccgtgc tcgcctggag ggcgccatga tcgtgcggtg ggagacgctc
     3121 gcgcaggcaa cggtggtcat cgtcgagggc cggctcgact tcggcgcgtc ccccggattt
     3181 cagcagcagc tcgaacaggc gctcgccggc gccggcacgc cgccggcggc ggtgatcatc
     3241 gactgcgcag cgctcgacta cgtctcgagc gccgggctgc gggtgttcct gctggcggcg
     3301 cgcgagtcgc agcgcctcgg tatcggcttt gcgctgtgcg cgctgcggcc ggcggtccgc
     3361 gaagtgttcg acctgagcgg gttcacccgc gtgattgcgg tgcacccgga ccggccgacc
     3421 gcgctcgagc ccccgtccgc atgaaggacc ggcacaccaa ggtcgcaagc gacgcggcgc
     3481 agctgccgct gctgatgcaa ttcctgcgcg agttctggtc ggctgccggc ctgccgccgg
     3541 cgcaggcgac ggccttcgag ctcgccctcg aggaggtctt catgaacgtc gtcaggcacg
     3601 gctcgccgca gggcaccgcg ccgtgggtcg aggtgtcgct ggcgctcacg gcagacggtc
//
LOCUS       DATDMP010000587         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_315450, whole genome shotgun sequence.
ACCESSION   DATDMP010000587 DATDMP010000000
VERSION     DATDMP010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315450"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>2151)
                     /locus_tag="VI195_10350"
     CDS             complement(168..>2151)
                     /locus_tag="VI195_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6824830.1"
                     /translation="FDTSAVISRRERDFMAVLDAHVAEFENSPGHAWLSLVRHDFQAA
                     ARLRRQIERYDEQSGAEWHSLLEDSAALIEGVQELLQRPARAGLLQAAQHAATAAEVA
                     EHTLRDTGAAVLALLAVVSVLLVGSISLPVRRLTAATRQFAGGDRAARARRGGSAEID
                     QLAESFNTMADRIASAEADLRAHQAELERHVAERTRQLHHLAHHDPLTQLPNRRKLST
                     HLEEALARAGSRQRLALLFVDVDNFKSINDTLGHSFGDRVLQHIAQRLHAATRSTGLL
                     ARLGGDEFTVLLEDVKSPEQVAEHAAQLVATLQQPLVVDGRVLTTSASVGASLYPDHA
                     PDAEGLLRAADVALFRAKELGRNRFALYSPALYDAAAQRFRLEQSLRRAVEGGELLLM
                     YQPQVSLSSFEATEVEALLRWRQPDGRIANAQEFIHVAERTGLIHDLTDWILHTAAAA
                     AATWRAQGWQRASVAVNVSAPQLFEHDFVENVGRTLEMTGLPASALELEITETVFQTG
                     PATIESLRRLRSLGVTIALDDFGIGYSSLTSLEQLPINRVKLDRLLIAGIDTNARSGA
                     IVRSIVALCHGLGLQVVAEGVERAAQLEFLSRCGPIGVQGFLLAHPVEESDTLREAQA
                     AGERARLALDTALQQGRREGADPLVYLGSSGRRRVT"
BASE COUNT          334 a          729 c          764 g          324 t
ORIGIN      
        1 atttcgtggg gtggcgttga cggggttccc ggccaattgc gcttctcgag gggcgtggac
       61 aactcgccac gcgggcgagg cgccattcgg cgggtcaggt ggcagcgcgg cgcgccgagc
      121 ttcagctcat cgccgcatgg gccgtgcgag ccgggcgggg cgctctgtca ggtgacgcgg
      181 cgccgcccgg agctgccgag gtacacgagc ggatctgcgc cctcgcggcg gccctgctgc
      241 agtgccgtat cgagagcgag ccgcgcgcgc tctccggcag cctgcgcctc ccgcaacgtg
      301 tctgactcct cgaccggatg cgcaagcagg aaaccctgca cgccgatcgg gccgcaacgg
      361 gaaagaaatt ccagctgcgc agcccgctca acgccctcgg cgacgacctg caaccccagt
      421 ccatggcaca gcgcgacgat ggagcgcacg atggcgcccg agcgcgcgtt ggtatcgatt
      481 ccggcaatga gcaggcggtc gagcttgacg cggttgatcg gcagttgctc gagcgaagtg
      541 agcgaggagt agccgatgcc gaaatcatcg agtgcaatgg tgacgccgag tgagcgcagg
      601 cggcgcagcg actcgatcgt cgccggtccg gtctggaaca ccgtctcggt gatctccagc
      661 tcgagcgcac tcgcgggcag cccagtcatc tccagcgtgc ggccgacgtt ctcgacgaag
      721 tcgtgctcga acaattgcgg ggcggacacg ttgacggcga cgctggcgcg ctgccagccc
      781 tgcgcgcgcc acgtggccgc agccgccgcg gcggtgtgca ggatccagtc ggtgagatcg
      841 tgaatgagac cggtccgttc ggcgacgtgg atgaattcct gcgcgttcgc gatacggccg
      901 tcgggctgtc gccagcgtag cagcgcctcg acttcggtcg cctcgaacga gctcagcgac
      961 acctgcggct ggtacatgag cagcagctcg cccccctcga ccgcgcgccg cagcgactgc
     1021 tcgagccgga accgctgtgc ggcggcgtcg tacagcgccg ggctgtaaag cgcgaagcgg
     1081 ttgcggccga gctccttggc acgaaacagc gccacgtccg ccgcgcgcag cagtccttcg
     1141 gcatccggcg cgtgatcggg ataaaggctg gcacccacac tggcactggt cgtgagcaca
     1201 cggccatcga ccacgagcgg ctgttggagc gtcgcgacca gttgtgcggc atgctccgcc
     1261 acctgctcgg gcgatttgac atcctcgagc agcaccgtga actcatcgcc gccgaggcgc
     1321 gcgagtagtc cggtgctgcg ggtcgcagca tgcaggcgct gtgcgatgtg ctgcagcacg
     1381 cggtcgccga agctgtgccc gagggtatcg ttgatcgact tgaagttgtc gacgtcgaca
     1441 aacagcagcg ccagccgctg gcggctgccg gcgcgcgcca gcgcttcctc gaggtgcgtg
     1501 gagagcttgc ggcgattcgg cagctgcgtc agcggatcgt gatgggccag atgatggagc
     1561 tggcgcgtgc gctcggccac gtggcgctcg agttccgcct gatgcgcgcg caggtccgcc
     1621 tcggccgagg cgatgcggtc cgccatggtg ttgaaggact cggcgagctg atcgatctcc
     1681 gccgagccgc cgcggcgcgc acgtgcggca cggtcaccgc cggcgaactg gcgggtcgcc
     1741 gcggtcaagc gccgcaccgg caggctgatg ctgccgacga gcagcaccga caccaccgcc
     1801 agcagtgcca gcaccgcggc gccggtatcg cgcaacgtgt gctcggcaac ctccgcggcg
     1861 gtggccgcgt gctgagctgc ctgcagcagt cccgcgcgcg cgggccgctg cagcagctcc
     1921 tgcacaccct cgatgagcgc cgcactgtcc tcgagcaggc tgtgccattc cgcgccgctc
     1981 tgctcatcgt agcgctcgat ctgccgccgt aagcgcgcgg ccgcctggaa gtcgtggcgc
     2041 acgaggctga gccaggcatg tccgggggag ttctcgaact ccgcgacgtg cgcatcgagc
     2101 accgccatga aatcgcgctc gcgacgcgag atgaccgccg aagtgtcgaa g
//
LOCUS       DATDMP010000588         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_426718, whole genome shotgun sequence.
ACCESSION   DATDMP010000588 DATDMP010000000
VERSION     DATDMP010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426718"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VI195_10355"
     CDS             <1..431
                     /locus_tag="VI195_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY6824831.1"
                     /translation="DIIVMEEHARFALPEINVGVLADAGTIKLRRRIPYHVAVEFLMT
                     GRWMDAAEAKHWGLANHVVPKGQGLAKAREIALQLAAGPPLLFPAIKQLLRHTEMVAE
                     HAAFEVHDSLDAVQKVVRSEDLKEGTRAFSEKRAPVWRGK"
     gene            complement(596..1759)
                     /locus_tag="VI195_10360"
     CDS             complement(596..1759)
                     /locus_tag="VI195_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY6824832.1"
                     /translation="MDFGLSEEQQLIIKTTRDFVRQELVPHEREVETTGVLREELWRP
                     LRAKALAAGLYAANMPAEVGGAGLDGVSWVLYEKELGYTNYALQYTCVARPSNILLAC
                     QDAQRERYLLPTVRGERIECLAMTEPQAGSDLRSMRTSAVQRGGDFIINGTKHFISHA
                     DHADFVILFAASGEEDSSRGKRKLITAFLIDKGTPGFEVLPGYHNVSHRGYNNCILHF
                     SDCRVPSSAVLGEVHRGFEVANTWLGATRLQVAANCLGRAERALECAKQWAADRVQFG
                     QTIGKFQGVSFKLADMAVELRAAELLTLEAAWKLDQGAATDMDMAIAKLKASEMLAMV
                     ADEALQIHGGMGLMSDLPLERIWRDARIERIWDGTSEVQRHIISRALLRPLGG"
     gene            complement(1787..2425)
                     /locus_tag="VI195_10365"
     CDS             complement(1787..2425)
                     /locus_tag="VI195_10365"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY6824833.1"
                     /translation="MPKIVDHEQRRDEVALIACRVVARYGFEQATIVRIARAAGCTTG
                     MIAHYFETKQDIIVAALRLILRRIEERLTHAAGAADLLTLLSEALPIDETRYIECAVW
                     MAFWGQVPSDKRLKRINGWLHREYLRMFERCLARSFHEWPRWPPATRAQVLRSVVTFI
                     NGVTASTVASRAEWPAARQIEQLRLQLELLHAWANGSSNRAVARGRRRLASA"
     gene            complement(2440..>2731)
                     /locus_tag="VI195_10370"
     CDS             complement(2440..>2731)
                     /locus_tag="VI195_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY6824834.1"
                     /translation="IDDAYRRSGRAMYTAESHLIHKAEAHSQEQLYVLSRVLEVDEKR
                     VRLLHALHRTRDDALVASGELLYLHVSTQGGRVSPMDAELQARLAAIQAATR"
BASE COUNT          431 a          907 c          934 g          459 t
ORIGIN      
        1 ccgacatcat cgtgatggaa gagcacgcgc gctttgcact gccggagatc aacgtcggcg
       61 tgctggccga cgccggcacg atcaagctgc ggcgccgcat tccctatcac gtcgcggtcg
      121 agttcctgat gaccgggcgc tggatggacg ctgcggaggc gaagcactgg ggcctcgcca
      181 accacgtagt gccgaagggt cagggcctcg ccaaggcgcg cgagatcgcg ctgcagctcg
      241 ccgcggggcc cccgctgctt ttcccggcga tcaagcagct gctgcgccat accgaaatgg
      301 tggccgagca tgcggccttc gaggtgcacg acagcctgga tgcagtgcag aaggtggtgc
      361 gctcggaaga cctcaaggag ggaacgcgcg cattttccga gaagcgtgcg ccagtgtggc
      421 ggggaaagtg aggcggcaag ccgcaggagc cgccagcccg ccgaggcggc agcgtgcggc
      481 ggccagtagg gacgggacgc cagttagggt ccttctgcgg gatagttcac cacttggtta
      541 actattctcg gaagtgcctt catacgccgc gggtcgcagg gcaccgaagc cgtactcaac
      601 ccccaagcgg ccgcagcagc gcgcgcgaga tgatgtgccg ctgcacctcg ctggtgccgt
      661 cccagatgcg ctcgatgcgc gcatcgcgcc agatgcgctc gagcggcaga tcactcatca
      721 gtcccatgcc gccgtggatc tgcagcgcct catcggcaac cattgcgagc atctcgctcg
      781 ccttgagctt cgcgatcgcc atgtccatgt cggtggcggc tccctgatcg agcttccagg
      841 cggcctcgag cgtgagcagc tcggcggcgc gcagctcgac tgccatgtcg gcgagcttga
      901 acgacacgcc ctggaacttg ccgatcgtct gtccgaactg cacgcggtcg gccgcccact
      961 gtttggcgca ctcgagcgcg cgctcggcgc gtcccaggca gttggcggcc acctgcaggc
     1021 gcgtcgcccc gagccaggtg ttggcgacct cgaagccgcg gtgcacttct ccgagcactg
     1081 cggaggatgg cacgcggcag tcgctgaagt gcaatatgca gttgttgtag ccgcgatggg
     1141 agacgttatg gtagccgggc agcacctcga agccgggcgt gcccttatcg atcaggaaag
     1201 cggtgatgag cttgcgcttg ccgcggctcg aatcctcctc gccactggcc gcgaacagaa
     1261 tgacgaagtc ggcgtggtcg gcgtggctga tgaaatgctt ggtaccgttg atgatgaagt
     1321 cgccgccgcg ctgcaccgcg ctggtgcgca tgctgcgcag gtcggagccg gcctgcggct
     1381 cggtcatagc gaggcattcg atgcgctcgc cgcgcaccgt gggcagcagg tagcgctcgc
     1441 gctgcgcgtc ctggcaggcg agcaggatgt tggacggacg agccacgcag gtgtactgca
     1501 aggcatagtt ggtgtagccg agctccttct cgtacagcac ccagctcacc ccatcgaggc
     1561 ctgcgccgcc gacttcggcc ggcatgttgg cggcgtacaa cccggcggcg agtgccttgg
     1621 ctctgagcgg ccgccacagc tcctcgcgca gcacgcccgt cgtttcgacc tcgcgctcgt
     1681 gcggtaccag ctcctggcgg acgaaatcgc gcgttgtctt gatgatgagc tgctgctctt
     1741 cgctcaggcc aaagtccatc ggtctctcct tgctgcgtgc gcgcctctag gccgaggcca
     1801 ggcggcgccg gccgcgcgcc acggcgcgat tggagctgcc gttcgcccag gcgtgcagca
     1861 gctcgagttg cagccgcagc tgctcgatct gccgtgcggc cggccactcg gcgcggctcg
     1921 ccacggtgct tgcggtcacg ccgttgatga aggtgacgac cgagcgcaac acctgggcgc
     1981 gcgtcgccgg cggccagcgc ggccactcgt ggaatgagcg cgccaggcaa cgctcgaaca
     2041 tccgcagata ctcgcggtgc agccagccgt tgatgcgctt caggcgcttg tcggaaggca
     2101 cttgccccca gaaggccatc cacaccgcgc attcgatgta gcgcgtctca tcgatcggca
     2161 gcgcttcact gaggagcgtc agcaggtcgg cggcgcccgc cgcgtgagtg agccgctcct
     2221 cgatgcgccg caggatgagc cgcagcgcgg cgacgatgat gtcctgcttg gtttcgaagt
     2281 agtgcgcgat catgccggtc gtgcagccag cggcacgggc gatgcgcacg atggtggcct
     2341 gctcgaagcc gtagcgcgct accacacggc aggcgatgag tgcgacctca tcgcgccgtt
     2401 gctcgtgatc gacgatcttg ggcatcggct cagggcgcct cagcgggtcg ccgcctggat
     2461 ggccgccaga cgtgcctgca gctccgcgtc catgggcgag actctgcccc cctgggtgct
     2521 cacgtgcagg tagagcagct cgccgctcgc caccagcgca tcgtcgcgcg tgcggtgcag
     2581 ggcgtgcagc aaccgcacgc gtttctcatc gacctcgagc acgcggctca ggacgtacag
     2641 ctgctcctgc gagtgcgcct cggccttgtg aatcagatgc gactccgccg tgtacatggc
     2701 acggcccgag cgccggtagg catcgtcgat a
//
LOCUS       DATDMP010000589         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1426928, whole genome shotgun sequence.
ACCESSION   DATDMP010000589 DATDMP010000000
VERSION     DATDMP010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426928"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VI195_10375"
     CDS             complement(<1..278)
                     /locus_tag="VI195_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824835.1"
                     /translation="MQDEVHEENGRRTPAQVSVSVGAGGYRAPGAPSSSRMFAAYLAD
                     IEQLLEAQRHDAALREALDLPRLAVALGDPQLRCTREQLQRWCEEWIRP"
     gene            complement(361..1026)
                     /locus_tag="VI195_10380"
     CDS             complement(361..1026)
                     /locus_tag="VI195_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824836.1"
                     /translation="MKKTGSEQSSTAEPLERVRLQADVAKQRVRIAKDELKRARKRLK
                     EAKREAKRARKLAAAARKAWKRARRAQKSGAEKVPASAKTRPRGSLAARKRPAASRRK
                     IRRRGTSTLVRRRNARRRASIARSRVRRRVRRIAASARMSQRRARVTPARIRRTASAP
                     LAAPAPRVVRRRAFARAPAAPPSAAVTPAVAHRPATSAAEPPVAPAVDNATVIVTPPD
                     QAL"
     gene            complement(1230..2267)
                     /locus_tag="VI195_10385"
     CDS             complement(1230..2267)
                     /locus_tag="VI195_10385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY6824837.1"
                     /translation="MPSGGHTTVIQRILERRAQQLPTSQEETTATLAVMLRVVPPAKT
                     LLLCQDAALRAQLEQRISRAMLECESVADEHEALQRCGAEHHAVVVTDNLALIRRLRA
                     RHSHRPPFVLYVAELDECAARAAGLGAGADECVARRAAERELDARLATARRIAELETV
                     LRVTLAENRKLSATDDLTRVASRRFFSKHFPREVERAARYGRALSLILCDIDLFKTIN
                     DSLGHAAGDQVLRQFGARLQKQLRRGIDWVARIGGEEFAVILPETGYEAALDVARKLR
                     AAIAQAHFTSEKKSVTVTASFGLCGLDRVPAGERRLAERVLKIADAALYRSKHSGRNR
                     VTATMFNAGTG"
     gene            2406..>2609
                     /locus_tag="VI195_10390"
     CDS             2406..>2609
                     /locus_tag="VI195_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824838.1"
                     /translation="MEPPGITRHRRPFLAPLYVSVLVAGVLFALGWVFYSNATTTVVF
                     LVHPVEKTPGSIDDPPLSPEGEAR"
BASE COUNT          364 a          902 c          890 g          453 t
ORIGIN      
        1 ggacggatcc actcctcgca ccacctttgc agttgctcgc gcgtgcagcg caactgcgga
       61 tcgcccaggg cgacggccag gcgcggcaga tcgagcgcct cgcgtagcgc cgcatcgtgc
      121 cgttgtgcct cgagcaactg ctcgatgtcg gcaagatagg cggcgaacat ccgcgagctg
      181 gagggcgcgc cgggggcccg gtagccgcct gcgcccaccg acacgctcac ctgagccggt
      241 gtgcggcgcc cgttctcttc gtgcacttca tcctgcatgt gtccaatccc ctcgttttat
      301 tggccgcatc cgctgcggct tacgctgcgc gccgccggag ccggcgcgcg gcgccatgtc
      361 tcagagcgcc tggtccgggg gcgtcactat caccgttgcg ttgtcgaccg cgggagcgac
      421 cggcggttcc gctgcggagg tggccggccg gtgggctacc gccggggtaa cggccgcgct
      481 tggcggcgcg gcgggagctc gggcgaatgc gcgtcggcgc accacccgag gcgcgggcgc
      541 ggccagcggt gcgctggccg tgcggcggat acgggccggg gtcacgcgtg cgcggcgctg
      601 gctcatgcgc gcggaagccg cgatccggcg cactcgccgg cgcacgcgac tgcgggcgat
      661 cgaggcgcgc cgcctcgcat tgcgccgccg cacgagcgtc gaagtcccgc gccgacggat
      721 cttccgccgc gaggcagccg gccgcttgcg cgccgcgagt gatccacgcg gtcgcgtctt
      781 ggcggaagct ggaactttct ccgctccgct cttctgagca cgccgggcgc gtttccaggc
      841 tttgcgggcg gcggcggcga gcttgcgcgc gcgcttggcc tcgcgcttgg cttctttcag
      901 ccgcttgcgg gcacgtttga gctcatcttt tgcgatgcgc acccgctgct tggcgacatc
      961 cgcctgcagg cgtacccgct cgagcggctc ggcggtggaa ctctgttcgc tgcctgtctt
     1021 cttcatccgg ggctcgcgaa gagaaaggac gcgagtctca cacaaggctc cgctcgtttc
     1081 cagcccggct acttatccac agaaattgtg cataactatg tggacagagc gcacacgcca
     1141 ctgcaaacgc cgatgtggcg caagggtttg catcgcgctg cgcgtgtttt cgccaattgc
     1201 gcgtgcgcgc accgcaccga gtcgcggctt cacccggttc cggcgttgaa catggtcgcg
     1261 gtgacccggt tgcgaccgga gtgcttgctg cgatagagcg ccgcatcggc gattttcagc
     1321 acgcgctcgg cgaggcgccg ctcgcccgct ggtacccggt cgagtccgca caacccgaag
     1381 ctcgcggtca ccgtgacact tttcttctcg ctggtgaagt gcgcctgcgc gatcgccgcg
     1441 cgcagcttgc gtgcgacatc gagtgccgcc tcgtaaccgg tttcggggag tatcacggca
     1501 aactcttcgc cgccgatgcg tgctacccag tcgatcccgc gtcgcaactg tttctgcaag
     1561 cgcgcaccaa actgccgcag aacctgatcg ccggccgcgt ggccgagcga atcgttgatg
     1621 gtcttgaaca gatcgatgtc gcacagaatg agcgacagcg ctcgaccgta gcgcgccgcg
     1681 cgctcgacct cgcggggaaa gtgcttgctg aagaagcggc ggctcgccac ccgcgtcaga
     1741 tcgtcggtgg ccgagagctt gcggttctcg gcgagcgtga ctcgcagcac cgtctcgagt
     1801 tcggcaatac gccgcgccgt ggcgaggcgc gcgtcgagct cgcgctcggc ggcgcgccgc
     1861 gcgacgcatt catcggcgcc ggcgccgaga ccggcggcgc gcgcggcgca ctcatcgagc
     1921 tcggccacgt acaggacgaa agggggacgg tgcgagtgcc gggcccgcag gcgccgaatc
     1981 aatgccaggt tgtcggtgac caccaccgca tggtgttcgg caccacagcg ctgcagtgcc
     2041 tcgtgctcgt cggcgaccga ctcgcactcg agcatggcgc ggctgatgcg ctgctcgagc
     2101 tgtgcgcgca gcgccgcgtc ctggcacagc agcagcgtct tcgccggtgg tacgacacgc
     2161 agcatcaccg caagggtggc ggtggtctct tcctggctcg tcggcagctg ctgcgcgcgc
     2221 cgctcgagga tacgttgtat gactgtggtg tgcccgccag atggcacggg tgcctcgctc
     2281 tctcgcccgg caagatgggc tgaaacggca gattaccgag gcgcgctcaa aaaagcgcgc
     2341 gttctggcgt cttgatgcgc agcagcagcc cggtctaccg ccccccacaa cgttatgatt
     2401 gccccatgga gccacccggc atcacccgcc atcgccggcc gtttctcgca ccgttgtatg
     2461 tcagtgtcct ggtagcgggc gtgctgttcg cgctcggctg ggtcttctac agcaacgcga
     2521 ccacgaccgt ggtgttcctc gtccacccgg tggagaagac tccgggctcc attgacgatc
     2581 cgccgctctc tcccgagggt gaggcgcgc
//
LOCUS       DATDMP010000590         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1870308, whole genome shotgun sequence.
ACCESSION   DATDMP010000590 DATDMP010000000
VERSION     DATDMP010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870308"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VI195_10395"
     CDS             <1..1365
                     /locus_tag="VI195_10395"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEY6824839.1"
                     /translation="VTAIELMPVAAFPGVRNWGYDGVLPYAPQASYGRPHELKALIAA
                     AHGQGLAVILDVVYNHFGPEGNYLHLYAPQFFDARRHTPWGAAINFDGEGSRVVRDFF
                     IHNALYWLEEYHLDGLRLDAVHAIHDRSEPHVIRQLAATARGAAGRTRSIYLTLENLH
                     NSARLLGVEGAPDTCDAQWNDDVHHCLHVLLTGESQEYYTDFSDSPHALLCRALAEGF
                     AYQGERSRLAGSSRGERSAHLPPSAFINFLQNHDQVGNRERGERLAQLVRDPAALRAA
                     SAVVLLAPSPPLLFMGEEWAAPQPFPWFCDFEPELLERVRAQRAREVPGAPDPGAVST
                     WAAAQLDWSQLRESAHARVLTHYRRLLTIRRRDIVPLLPYLGAGRCTRAQRGVAFAVD
                     WSGRGETLHLIANLAAAPAPLPSRAAGRVVFATHPGVRATLARNELAPWSVLWLLEQR
                     GESA"
     gene            1352..>2062
                     /locus_tag="VI195_10400"
     CDS             1352..>2062
                     /locus_tag="VI195_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY6824840.1"
                     /translation="MNPPERPYLVRRRGLEQADGAAVIPRATYRLQLNAGFTFRDALG
                     VVPYLAALGVSHLYCSPYFRARSGSSHGYDVVDHNSFNPEIGDEADFERLVAALRARG
                     MGHIVDIVPNHVGILGTDNAWWMDLLENGESSAYAGFFDIDWAPANPDRAHKILVPVL
                     ADPYATALERGELELRFERDSGSFAVFYGAHRLPLDPRTYPRILDRVAALVSSSELEN
                     IRRALAALPDRRAPTPEQV"
BASE COUNT          286 a          739 c          690 g          347 t
ORIGIN      
        1 gtgaccgcca tcgagctcat gccggtcgcc gcctttcccg gagtgcgcaa ctggggttac
       61 gacggtgtgt tgccgtacgc acctcaggcc tcctatggcc gcccgcacga gctcaaggcg
      121 ctgatcgcgg ccgcccatgg ccagggactc gcggtaattc tcgatgtggt ctacaaccac
      181 tttggccccg agggcaacta cctgcacctg tacgctccgc agttcttcga tgcgcgccgc
      241 cacactccgt ggggcgcagc catcaacttc gatggcgagg gcagccgcgt ggtgcgcgac
      301 ttcttcattc acaacgcgct ctattggctc gaggaatacc acctggatgg cctgcggctc
      361 gatgcggtgc acgccattca cgatcgcagc gagccgcacg tcatcaggca gctggccgct
      421 accgcgcgcg gcgccgcagg ccgcacgcgc agcatctatc tgacgctcga gaatctgcac
      481 aacagcgcgc ggctgctggg cgtggaaggt gcgcccgaca cctgcgatgc gcagtggaac
      541 gatgacgtgc atcactgtct gcacgtgctg ctcaccggcg agagccagga gtactacacc
      601 gatttcagcg attcaccgca cgcgctgctg tgccgcgcgc tcgccgaggg cttcgcctat
      661 cagggggagc gctcgcgcct tgccggcagc tcccgtggcg agcgcagcgc gcacctgccc
      721 ccgagcgcct tcatcaattt tctgcagaat cacgaccagg tcggcaaccg cgagcgcggc
      781 gagcggctgg cgcagctggt acgcgatccg gccgcgttgc gggcggcgag cgcggtcgtg
      841 ctgcttgccc cgtcgccgcc gctgctgttc atgggggagg aatgggcggc gccgcaacct
      901 ttcccgtggt tctgcgactt cgagccggag ctgctcgagc gcgtccgcgc gcagcgcgcg
      961 cgcgaggtcc ccggcgcgcc ggatccgggc gcggtgagta cctgggccgc cgcgcagctc
     1021 gactggagcc agttgcgcga gagcgcgcac gcgcgcgtgc tcactcacta ccgccggctg
     1081 ctcaccatcc ggcggcgcga catcgtgccg ctgctgccat acctgggcgc cggccgctgc
     1141 acgcgggcgc agcgcggcgt tgccttcgcg gtcgactgga gcggccgcgg cgaaaccctg
     1201 catctcatcg ccaatctggc cgcagcgcct gcgccgctac cctcacgcgc ggccggtcgc
     1261 gtggtgttcg ccactcaccc cggggtgcgc gcgacgctcg cgcgcaatga gcttgcgccc
     1321 tggagcgtgc tgtggctgct ggagcagcgc ggtgaatccg cctgaacgac cctacctggt
     1381 gcgtcgccgc ggcctcgagc aggccgatgg cgctgcggtc atcccgcggg cgacctatcg
     1441 gctgcagctc aacgccggct tcactttccg cgacgctttg ggggtcgtgc cgtatctcgc
     1501 ggcgctcggc gtcagtcacc tctattgctc accgtatttc cgggcgcgtt ccggcagttc
     1561 ccatggctac gacgtggtcg atcacaactc cttcaatccg gagatcggcg atgaggccga
     1621 cttcgagcgc ctggtggcgg cgctgcgcgc gcgcggcatg gggcacatcg tcgacatcgt
     1681 cccgaaccat gtgggcatcc tcggcaccga caacgcctgg tggatggatc tgctggagaa
     1741 tggcgagtcg tcggcttacg ccgggttctt cgacatcgac tgggcgccgg ccaaccccga
     1801 ccgtgcgcac aagatcctgg tgccggtgct cgccgacccg tacgccaccg ctctcgagcg
     1861 cggcgagctc gagctgcgtt tcgagcgcga ttccggctcg ttcgcggtgt tttacggagc
     1921 gcaccggctg ccgctcgacc cgcgcaccta tccgcgcatc ctcgaccgcg tggcggcgtt
     1981 ggtgtcgagc agcgagctcg agaacatccg ccgtgcgctc gcggcgctgc ccgaccggcg
     2041 cgcaccgacc ccggaacagg tg
//
LOCUS       DATDMP010000591         2413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_648841, whole genome shotgun sequence.
ACCESSION   DATDMP010000591 DATDMP010000000
VERSION     DATDMP010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648841"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VI195_10405"
     CDS             complement(<1..675)
                     /locus_tag="VI195_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY6824841.1"
                     /translation="MNRAPPRELRAGALGTYRRLLGYLRPHRGRFLVGILGGVLYSAT
                     MASFALLAKKFGDGTFTHHDPRTIVWVPLAVVGLFLLRGIGDFTQIYFMGYVGRQIVS
                     RLRGQVFHRILELPIGFFDRNSSAMLLSRLTYNTEQIGQATTDSVIVVVRTTLTILAS
                     IGFLLWLNARLALLALVMGPLVGWLVTVINRHFRRYSRRIQDSMGDVTRVAKESFEAP
                     RLIKVYN"
     gene            complement(672..1103)
                     /locus_tag="VI195_10410"
     CDS             complement(672..1103)
                     /locus_tag="VI195_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY6824842.1"
                     /translation="MNLKPRRHEEPEINVVSLIDVVLLLVVFFILSSRFSDEGRLRVH
                     LPRASAVPTEKVGSEPLVVSVTEQGSYYVNQHELINASPETLRAALLKEAGERRDMPV
                     TLRADARATHQSVITAMDVLGRLGFAEVNLATVKEAPAGKP"
     gene            complement(1129..1734)
                     /locus_tag="VI195_10415"
     CDS             complement(1129..1734)
                     /locus_tag="VI195_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY6824843.1"
                     /translation="MWPIILCSITAAAIILERLWTLQDRRVLPRELPQRVWQLIEANQ
                     VNDKVIAALEQNSPLGRLLATGLANRHRPREILMERLEDTGRHVVHELERFLNTLGTI
                     AGVSPLLGLLGTVTGIIRSFNVIEAGGMGDPRALSGGIAEALICTVAGLTVAIPALIF
                     YRYLRGRVDGIVVEMEKHAIRMADALEQARLRERASPAAAA"
     gene            1933..>2413
                     /locus_tag="VI195_10420"
     CDS             1933..>2413
                     /locus_tag="VI195_10420"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY6824844.1"
                     /translation="MPAPDALRERWAVRVFGDRVADPQLWTLHRRGVTYAFGAGIAIC
                     FVPLPVHLLLCVCVALACRLNLPVMYGATWLFTNPFTAVPVYYLAYRVGAALLHRPRQ
                     HFRFVADWRWFTHGLEPVWQPFFLGCLVCAVVGGLLGWLLLEVLWRWQVTSRYRARHS
                     "
BASE COUNT          364 a          826 c          841 g          382 t
ORIGIN      
        1 gttgtagacc ttgatgagcc gcggcgcctc gaagctctcc ttggcgacgc gcgtgacgtc
       61 gcccatggaa tcctggatgc ggcggctgta acggcggaag tgccggttga tcaccgtcac
      121 cagccagccg accagcggcc ccatgacgag cgcgagcagc gcgaggcgcg cattcagcca
      181 cagcaggaag ccgatcgagg cgaggatggt gagcgtggtg cgcaccacca cgatcaccga
      241 gtcggtggtt gcctggccga tctgctcggt gttgtaggtg agccgcgaga gcagcatggc
      301 cgaggagttg cggtcgaaaa agccgatggg gagctcgagg atgcggtgga acacctggcc
      361 gcgcagccgg ctcacgatct gccgcccgac gtagcccatg aagtagatct gggtgaagtc
      421 gccgatgccg cgcagcagga acaacccgac caccgccagc ggcacccaca cgatggtgcg
      481 cggatcgtgg tgcgtgaagg tgccgtcgcc gaatttcttg gcgagcagcg cgaagctcgc
      541 catggtggcc gagtaaagca cgccaccgag gatcccgacc aggaagcggc cgcggtgcgg
      601 ccgcaggtag ccgagcaggc ggcggtaggt gccgagcgcg ccggcgcgca gctcgcgggg
      661 gggcgcacgg ttcaaggctt gcctgccggc gcttccttga cggtggcgag gttgacctcg
      721 gcgaatccga ggcgtccgag cacgtccatg gcggtgatga cggactgatg ggtggcgcgg
      781 gcatcggcgc gcagcgtgac cggcatgtcg cgccgctcac ccgcctcctt gagcagcgcc
      841 gcgcgcagcg tctcgggact cgcgttgatg agctcgtgct ggttcacgta gtagctgccc
      901 tgctcggtga cgctcaccac cagcggctcg ctgcccacct tctcggtcgg caccgcgctg
      961 gcgcgcggca gatgcacgcg cagccgtccc tcgtcgctga agcggctgga gaggatgaag
     1021 aacaccacca gcagcagcac cacgtcgatg agcgagacca cgttgatctc cggctcctcg
     1081 tggcggcgcg gcttgagatt catgccggtc ccgggccccg cggtcctctc aggcggccgc
     1141 cgctggcgag gcgcgctcgc gcagccgcgc ctgctcgagc gcatcggcca tgcggatcgc
     1201 gtgcttctcc atctcgacca cgatgccgtc gacccggccg cgcaggtagc gatagaagat
     1261 gagcgccgga atcgcgacgg tgaggccggc cacggtgcag atcagcgcct cggcgatgcc
     1321 gcccgagagc gcgcgcgggt cgcccatgcc ccccgcctcg atcacgttga aggagcggat
     1381 gatgccggtc acggtgccga gcaggccgag cagcggcgac acgcccgcga tggtgccgag
     1441 cgtgttgagg aagcgctcca gctcgtgcac tacgtgccgc ccggtgtctt cgagacgttc
     1501 catgaggatc tcgcgcggcc ggtggcggtt ggcgagcccg gtggcgagca gccggccgag
     1561 cggggagttc tgctcgagcg ccgcgatcac cttgtcgttc acctggttgg cctcgatcag
     1621 ctgccagacc cgctgcggca gctcgcgcgg cagcacccgc cggtcctgca gcgtccacag
     1681 ccgctcgaga atgatggccg ccgccgtgat cgagcacaga atgatcggcc acatcagcgg
     1741 gccgccggcc cgcactattt cccacatcct tcgcgacccc gcagcaggaa aaatctgcaa
     1801 aggcggcatc ataccggagg gccgcgcccg ctcaaggcac gagctcacgg acgcggcgtg
     1861 ccgcacatat tgccgtccgg gccttctggt acagtccccg gctcgatgcc ccgccagttc
     1921 ctgaaaaaga tcatgcccgc gcccgatgcg ctgcgggagc gctgggcggt gcgcgtattc
     1981 ggcgatcgcg tcgccgatcc gcagctgtgg accctgcacc gccgcggcgt cacgtacgcc
     2041 ttcggcgccg gtatcgccat ctgtttcgtg ccgctgccgg tgcacctgct gttgtgcgtg
     2101 tgcgtcgcgc tcgcgtgccg gctcaacctg ccggtgatgt acggggccac ctggctgttc
     2161 accaacccct tcactgcggt gccggtctac tacctcgcgt accgggtcgg agcggcgctc
     2221 ctgcaccggc cgcgccagca tttccgcttc gtggccgact ggcgctggtt cacccacggt
     2281 ctggaaccgg tctggcaacc cttcttcctg ggatgcctag tatgtgccgt ggtcggcggc
     2341 ctcctcggct ggctgctgct ggaggtgctg tggcgctggc aggtcaccag ccgctaccgc
     2401 gcgcgccact cgg
//
LOCUS       DATDMP010000592         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_981578, whole genome shotgun sequence.
ACCESSION   DATDMP010000592 DATDMP010000000
VERSION     DATDMP010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981578"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VI195_10425"
     CDS             <1..380
                     /locus_tag="VI195_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6824845.1"
                     /translation="DLGLTSRRDQLAGSLSGGWKQRLALASCLLHKPKLLLLDEPTAG
                     VDPKARRDFWDELHRMCEQGMTVLVSTHYMDEAERCHQLGYIAYGKLLALGTAAELLQ
                     THNAPTLEALFVQLMKGTEDNFQ"
     gene            377..1528
                     /locus_tag="VI195_10430"
     CDS             377..1528
                     /locus_tag="VI195_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824846.1"
                     /translation="MSAFSSLSRPSLSRWIGIMLKEFIQLKRDRLTFGMIIGIPVIQL
                     ILFGYAINSDPKQLPTAVLMADHGYYGRTLLWAMRNSGYFAIASEVRNEEEANALLAE
                     AKVQFVITVPENFSRKVARGERPPLLVEADATDPSAIGNAVAALTGIARSALARDLQG
                     SLARLNPEPDPVELRVHRRYNPENVTQYNIVPGLLGVILTMTMILMTGLAMTRERERG
                     TFENLLATPALPIEVMTGKIIPYILIGLIQVTIVLGASQVLFHVPMLGSAPLLYACVL
                     LFIAANLALGITFSSIARNQLQAMQMTFFFFLPSMLLSGFMFPFRGMPEWAQWIGSIL
                     PLTHFLQLVRGIMLKGNEVAMLWPHIWPLFAFMFGAIGLGLLVYRRTLD"
     gene            1541..1969
                     /locus_tag="VI195_10435"
     CDS             1541..1969
                     /locus_tag="VI195_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HEY6824847.1"
                     /translation="MRLWSLHPRYLDSRGLVALWRESLLAQAVLRGETRGYRHHPQLD
                     RFRSHPASLAAMSLYLSAIHAEAASRGYSFDKRKIRPARQRATLSVTSGQMRYEWSHL
                     LAKLKVRDPALCRKWRAILAPAPHPLFRVRAGEIESWERR"
     gene            1971..2264
                     /locus_tag="VI195_10440"
     CDS             1971..2264
                     /locus_tag="VI195_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6824848.1"
                     /translation="MKPDPRPKAGSKLLQENERVIVTEWRFAPGAETGWHRHAHDYVV
                     VCLTAGKLLAETSAGNVETELQFGQAYARPVGVEHNIVNSGSAEFVFVEVEIK"
BASE COUNT          381 a          769 c          734 g          412 t
ORIGIN      
        1 aggatctcgg cctgacgtcg cgccgcgacc agctcgcggg atcgctttcg ggcggctgga
       61 agcagcgcct tgcgctcgcc tcctgcctgc tgcacaagcc gaagctgctg ctgctggacg
      121 agcccaccgc gggcgtcgat cccaaggcgc ggcgcgactt ctgggacgag ctgcaccgca
      181 tgtgcgagca gggcatgacg gtgctcgtct cgacgcatta catggacgag gcagagcgct
      241 gccaccagct cggctacatc gcctacggca agctgcttgc gctcggcacc gcggcggagc
      301 tcctgcagac gcacaatgcg cctacgctgg aagcgctgtt cgtgcagctc atgaagggaa
      361 ccgaggacaa cttccagtga gcgcattttc ctctctgtcg cgcccgtcgc tgtcgcgctg
      421 gatcggcatc atgctgaagg agttcatcca gctgaagagg gatcggctca ccttcggcat
      481 gatcatcggc atcccggtga ttcagctgat cctcttcggc tacgcgatca actcggatcc
      541 gaagcagctg ccgacggcgg tgctcatggc cgaccacggc tactacggcc gcacgctgct
      601 ctgggcgatg aggaacagcg gctatttcgc gattgccagc gaggtgcgca acgaggaaga
      661 ggcgaacgcg ctgctcgccg aggccaaggt gcagttcgtg atcaccgtgc cggagaattt
      721 ctcgcgcaag gtggcgcgcg gcgagcgccc gccgctgctg gtcgaggcgg acgcgaccga
      781 cccctcggcc atcggcaacg cggtcgcggc gctcaccggc atcgcgcgca gcgcgctcgc
      841 gcgcgacctg cagggctcgc tcgcgcgcct caatcccgag cccgacccgg tcgagctgcg
      901 cgtgcaccgg cgctacaacc ccgagaacgt cacgcagtac aacatcgtgc ccgggctgct
      961 cggcgtgatc ctgacgatga ccatgatcct catgaccggt ctcgccatga cgcgcgaacg
     1021 cgagcgcggc accttcgaga acctgctcgc cacgcccgcg ctgccgatcg aggtgatgac
     1081 cggcaagatc atcccctaca tcctcatcgg cctgatccag gtgacgatcg tgctgggggc
     1141 ctcgcaggtg ctgttccacg tgccgatgct cggctccgcg ccgctgctct acgcctgcgt
     1201 gctgctgttc atcgccgcca acctcgcgct cggcatcacc ttctcctcga tcgcgcgcaa
     1261 ccagctgcag gcgatgcaga tgaccttctt cttcttcctg ccctcgatgc tgctctcggg
     1321 cttcatgttc ccgttccgcg gcatgcccga atgggcgcaa tggatcggct cgatcctgcc
     1381 cctcacgcac ttcctccagc tcgtgcgcgg catcatgctg aagggcaatg aagtggcgat
     1441 gctctggccg cacatctggc cgctcttcgc cttcatgttc ggcgccatcg gcctcggcct
     1501 gctcgtctac cggcgcacgc tggactagcc ggccctccga atgcggctct ggtcgttgca
     1561 tcccagatac ctggattcgc ggggattggt cgcgctgtgg cgcgaatcgc ttctggcgca
     1621 agcggttctg cgcggagaaa cgcgcggcta tcggcaccat ccgcagctgg atcgattcag
     1681 gagccacccg gcgtcgttgg cggccatgtc cctgtacctg agcgccattc acgcggaggc
     1741 tgcatcgcgt ggctattcgt tcgacaagcg caagatccgg ccggcgcggc agcgggcgac
     1801 actttccgtc acgtccggcc agatgcggta cgaatggtcg catctgctgg ccaagctcaa
     1861 ggtccgagat cctgcgctgt gccgcaagtg gcgggctata ttggcgccag cgcctcaccc
     1921 tttgttcaga gttcgagcag gagaaatcga atcatgggag cgccgatgac atgaagcccg
     1981 atccgcgccc gaaagccggc agcaagttgt tgcaggaaaa cgagcgggtc atcgtcaccg
     2041 agtggcgctt tgcgccgggc gccgagaccg gttggcaccg ccacgcccac gactacgtcg
     2101 tcgtctgcct gaccgcaggg aagctgctgg cggaaacgtc tgccgggaac gtggagaccg
     2161 aactgcagtt cgggcaggca tatgcgcgtc cggtgggtgt agagcacaac atcgtcaatt
     2221 ccggaagcgc ggagttcgtg ttcgtcgagg tggagatcaa atgaggtcgt ggcctagcag
     2281 ccggccgcgt gaagcg
//
LOCUS       DATDMP010000593         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_537786, whole genome shotgun sequence.
ACCESSION   DATDMP010000593 DATDMP010000000
VERSION     DATDMP010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537786"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VI195_10445"
     CDS             complement(<1..928)
                     /locus_tag="VI195_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY6824849.1"
                     /translation="MKYHDLRDFLAQLEARGNLARVRTAVDPHLEMTEICDRVLRDAG
                     PAILFEHPKGHTIPVLGNLFGTPQRVAWGMGQESPDALRELGKLLAFLKEPEPPKGLI
                     DAWRNTRPVFMQVLNMAPKLRTSAACQEIVWEGADVDLSRLPIQTCWPGDAGPLITWG
                     LTVTRGPHKARQNLGIYRQQVIGRNRVIMRWLAHRGGALDARDHALANPGTRFPVAVA
                     LGADPATILGAVTPVPDTLSEYQFAGLLRGGRTEIVKCITHDLQVPASAEIVLEGFLN
                     PDRSEPAGYEMAAEGPFGDHTGYYNEVERFPVL"
     gene            complement(957..2000)
                     /locus_tag="VI195_10450"
     CDS             complement(957..2000)
                     /locus_tag="VI195_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6824850.1"
                     /translation="MSGIESRDRRRWLIGAGALGAGTTLASNTVAAAALQPTNAGDPQ
                     LLAISAREFWADKNGVRLWVYRKRVNTAAPSPNGLLFLVHGSSYSGKTMFDLSVPNRT
                     DYSFMDHFARLGFDVWTMDHEGYGHSDRTSSNSDIASGAEDLKIAMRVIERETGQARA
                     AYFGQSSGALRAGLFANLYPQHVSKLMLDALVWTGKDAPTLAQRKKMLPQLQASNVRK
                     IDREFYASVFTRDDPNAAERMLGDIVADAELKYGSTVPTGTYLDMVTKLPIVDPTRIQ
                     CPVLITRAEHDGIATDEDVIGFFERLPNSDKQLVKIGGLAHTAPLGINRHRFWHALYA
                     FLTMPDRRPAGAA"
     gene            complement(2025..>2485)
                     /locus_tag="VI195_10455"
     CDS             complement(2025..>2485)
                     /locus_tag="VI195_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6824851.1"
                     /translation="RDRVSLMGWANGASALLWAVRPQSLARGPAPDFRAAIAFYPGCY
                     DSANAREGYAPAAPLLMLVGAEDDWTSPRPCVALGERMRRVSQPIEVVVYPDTYHGFD
                     GPDSQGRMHLDMPNGVHPGEGVTVAVNRAAREDAYRRVKAFLRAVLAPGQ"
BASE COUNT          407 a          848 c          819 g          411 t
ORIGIN      
        1 tgagcaccgg aaaacgctcg acctcgttgt agtagcccgt gtgatcgccg aagggaccct
       61 ctgctgccat ttcgtagccg gcgggctcgc ttcgatcagg gttgagaaaa ccctcgagca
      121 cgatctccgc cgatgccggc acctgcagat cgtgcgtgat gcatttcacg atctcggtgc
      181 gacccccgcg cagcagaccc gcgaactggt actccgacag cgtgtcgggc accggcgtca
      241 ccgcgccgag gatggtcgca ggatccgcgc ccagcgccac tgcgaccgga aaacgcgtgc
      301 cggggttggc cagcgcgtga tcgcgcgcat cgagcgcgcc gccgcggtgc gcgagccagc
      361 gcatgatcac gcgattgcgc ccgatcacct gctggcgata gatgccgaga ttctgccgcg
      421 ccttgtgcgg acctcgcgtg acggtgagac cccaggtgat caatggaccc gcatcgcccg
      481 gccagcaggt ctggatcggc aggcgcgaaa gatcgacatc ggcgccttcc cacacgatct
      541 cctggcacgc cgcgctggtg cgcagcttcg gcgccatgtt cagcacctgc atgaacaccg
      601 gccgggtgtt gcgccaagcg tcgatcagac ccttgggcgg ctccggctcc ttgaggaacg
      661 cgagcagctt gccgagttcg cgcaacgcat cgggcgactc ctggcccatg ccccacgcga
      721 cccgctgcgg cgtgccgaac aggttgccga gcacgggaat cgtgtggcct ttcggatgct
      781 cgaacaggat cgccggaccg gcatcgcgca acacgcgatc gcagatctcc gtcatctcga
      841 gatgcggatc gacggccgtg cgcacgcgcg cgagattgcc gcgcgcctcg agctgcgcga
      901 ggaaatcgcg caggtcgtgg tacttcaagt cgcggacgac gctggcgcca ggcgcttcac
      961 gccgcgcccg ccggtcgtcg atcgggcatg gtgaggaacg cgtacagggc gtgccagaag
     1021 cgatgccggt tgatgccgag cggcgcagtg tgcgcgaggc cgccgatctt cacgagctgc
     1081 ttgtcggaat tcggcaaccg ctcgaagaac ccgatcacat cctcgtcggt ggcgatgcca
     1141 tcgtgctccg cgcgcgtgat cagcacgggg cactggatcc tggtcggatc cacgatcgga
     1201 agcttggtca ccatgtcgag ataggtgccg gtcggcacgg tgctgccgta cttgagctcg
     1261 gcatcggcca cgatgtcgcc cagcatgcgc tcggcggcat tcggatcgtc gcgcgtgaac
     1321 acgctcgcgt agaactcgcg atcgatcttg cgcacgttcg atgcctgcaa ctgcggcagc
     1381 atcttcttgc gctgcgccag cgtcggggcg tccttgccgg tccacaccag cgcatcgagc
     1441 atcagcttcg agacgtgctg cggatacagg ttggcgaaga gcccggcgcg caatgcacca
     1501 gacgactggc cgaaataggc cgcgcgcgct tgtcccgtct cacgctcgat cactcgcatc
     1561 gcaatcttga gatcctcggc gccgctcgcg atgtccgaat tcgagctcgt gcgatccgag
     1621 tggccgtagc cttcgtgatc catcgtccac acgtcgaagc cgaggcgagc gaagtgatcc
     1681 atgaacgagt aatcggtgcg gttcggcacg ctcaggtcga acatggtctt gcccgagtac
     1741 gagctgccgt gcaccaggaa cagcaggcca ttcggtgacg gcgccgcggt gttcacgcgc
     1801 ttgcgataga cccacagccg cacgccgttc ttgtcggccc agaattcgcg cgcgctgatg
     1861 gcgaggagtt gcgggtcgcc tgcgttggtc ggctgcaacg cggccgctgc caccgtattg
     1921 ctcgcgagcg tggtgcctgc accgagcgct cccgcgccga tcaaccagcg ccgccggtcg
     1981 cgactttcga tcccgctcat cacttcttct cctccgcaga acttctactg gccgggtgcg
     2041 agcacggcgc gcaggaatgc cttgacgcgc cggtatgcgt cttcgcgcgc cgcgcgattc
     2101 accgcaaccg tgacaccctc gccaggatgc acgccgttgg gcatgtccag gtgcattcgg
     2161 ccctggctgt cggggccgtc gaagccatga tacgtgtccg gatacaccac cacctcgatg
     2221 ggctgcgaca cacgccgcat gcgctcgccg agagcgacgc agggccgcgg cgaggtccag
     2281 tcgtcctccg ccccgacgag catgagcagc ggcgcggcgg gcgcatagcc ttcccgggcg
     2341 ttggccgagt cgtagcagcc cggatagaac gcgatcgccg cgcggaaatc gggcgccgga
     2401 ccgcgcgcca ggctttgcgg ccgcacggcc cagagcagcg cgctggcgcc gttggcccag
     2461 cccatcaggc tgacgcggtc gcgcg
//
LOCUS       DATDMP010000594         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1760378, whole genome shotgun sequence.
ACCESSION   DATDMP010000594 DATDMP010000000
VERSION     DATDMP010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760378"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VI195_10460"
     CDS             complement(<1..805)
                     /locus_tag="VI195_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6824852.1"
                     /translation="MARPLPKSIDETHKLLGEADYVADRSLATALFLSLSMQRPLFLE
                     GEAGVGKTEIAKVISAALGRELLRLQCYEGLDIAQAAYEWNYSRQMIEIRMAEAEGVK
                     NREGLSQDIFAEKFLIRRPLLRALQPTGAPPVLLIDELDRTDEPFEAYLLEVLSDWQI
                     TVPEIGTIRADEPPIVVITSNRTREIHDAVKRRCFYHWVDYPDAAREFEILRRKAPKA
                     AGALSREVVEFVQRLRSVDLFKLPGVAETIDWANCLVALDRVTLDPETVN"
     gene            1057..1986
                     /locus_tag="VI195_10465"
     CDS             1057..1986
                     /locus_tag="VI195_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6824853.1"
                     /translation="MLLPALALAQGATRLSRIGVLSPFIGPDSAFFEVLRLRLQELGY
                     VEGKNAAFIYRAAEDFEALSRHAVEMARLQADVIVTAGAPGVRAAMSASKTVPIVIAN
                     VGDAVAQGFVASLARPGGNVTGLTSLNTELSVKRLDLLAEALPSLRQVVALGEAVGDS
                     GPIHATEEAARARGIKLEVMRVREADELPSAFAAMPAGRTTALCVIQGPLFTSQLRRI
                     VELAAARGLPAMYPDSRFVEVGGMMSYGPNIREIYRQSAGYVDRILKGAVPESMPVEQ
                     PSVFELALNLKTVRNLGVALSREVRTRADIVIE"
BASE COUNT          328 a          658 c          712 g          364 t
ORIGIN      
        1 tgttgacggt ctcggggtcg agcgtcacgc ggtcgagcgc gacgaggcag ttcgcccagt
       61 cgatggtttc cgccacgccg ggcagcttga acaggtccac gctgcgcaag cgctgcacga
      121 actcgaccac ttccctggac agcgcgcccg ccgccttggg cgccttgcgg cgcaggatct
      181 cgaattcgcg cgccgcgtcc gggtagtcca cccagtggta gaagcagcgg cgcttgaccg
      241 cgtcgtggat ctcgcgcgtg cggttggagg tgatcaccac gatcggcggc tcgtcggcgc
      301 ggatggtgcc gatctccggc accgtgatct gccagtccga gagcacttcc agcaggtagg
      361 cctcgaacgg ctcgtcggtg cggtcgagct cgtcgatgag cagcaccggc ggcgcgcccg
      421 tcggctgcag cgctcgcagc agcggccgac ggatgaggaa cttctccgcg aagatgtcct
      481 gcgagagacc ttcgcggttc ttcacgccct cggcctcggc catgcggatc tcgatcatct
      541 ggcgcgagta gttccactcg taggccgcct gcgcgatgtc caggccctcg tagcactgca
      601 gccgcagcag ctcgcggccg agcgccgcgg aaatcacctt ggcgatctcg gtcttgccca
      661 cgcccgcttc gccctcgagg aacagcggcc gctgcatgga gaggctcaag aacagtgccg
      721 tcgccaggct gcggtcggcc acgtaatcgg cctcgccgag cagcttgtgg gtctcgtcga
      781 tggacttggg caacggtcgg gccatgggca ggaagcgtgt cggagtgcgg aaagcgatca
      841 ttttcgggca tttggctctc gtccgccacc gctggctcgc aacgtcccga atttgcatct
      901 gcacgcggca ttggcaggct ccaaaccgtc ctgcgcacgg acctactgaa cgaatgcgcc
      961 aggttcggca agatctccgc ggactagtca tagcggggcc ggtgatagag tggatgcgtg
     1021 cggcgtagat tgattcttct tggaggcgcc ggcgcgttgc tcctgccggc gctggcgtta
     1081 gcccaggggg cgacccggct ctcgcgcatt ggcgtgctgt cgccgttcat cgggccggac
     1141 agcgcatttt tcgaggtgct gcggttgcgg ctgcaggaac tcggctatgt ggaaggcaag
     1201 aacgcggcat tcatctaccg tgcggcggag gactttgagg cgctctcgcg ccatgcggtg
     1261 gagatggcac gcctgcaggc ggacgtgatc gtgacagcgg gagcgccggg cgtgcgcgct
     1321 gccatgagcg cgagcaagac cgttcccatc gtcatcgcca atgtcggcga cgcggttgca
     1381 caagggttcg tcgcgagctt ggcgcggccc ggaggcaacg tcactgggct cacttcgctg
     1441 aataccgagc tgagcgtaaa gcggctcgac ctgctggccg aggcgctgcc gtcgctccgg
     1501 caggtggtcg cgctgggaga ggcggtggga gattcgggcc ccattcatgc gacagaggag
     1561 gcggcgcgcg ctcgcggcat caagctcgag gtgatgcggg tgcgcgaggc cgacgagctg
     1621 cccagcgcgt tcgccgccat gcccgctggc cgcacgacgg cactgtgcgt gatccagggc
     1681 ccgcttttta ccagccagct gcgtcgcatc gtcgaacttg cggcggcccg cggcctgccc
     1741 gccatgtatc cggattcacg cttcgtcgaa gtcggcggga tgatgagcta cggcccgaat
     1801 atccgggaaa tctaccgcca gtcagcgggc tacgtcgacc ggattctcaa aggggcggtg
     1861 ccggaatcga tgccggtcga acagccgtcc gtcttcgagc tcgcgctcaa tctgaagacg
     1921 gtgcgcaacc tcggagtggc gctgtcgcgc gaagtgcgca cacgagccga catcgtgatc
     1981 gagtagcctg cggctacttg agccagatga tctccgacgc gccctctgcg ctgatgttcg
     2041 cctgcaacag aaccaatgga ac
//
LOCUS       DATDMP010000595         2862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1427720, whole genome shotgun sequence.
ACCESSION   DATDMP010000595 DATDMP010000000
VERSION     DATDMP010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427720"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VI195_10470"
     CDS             <1..416
                     /locus_tag="VI195_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824854.1"
                     /translation="GEDFFVLNGDSYLPCSLPSIQAAYRAARRPALMTIMRNDNRWDR
                     SNVLFSGGRLLAYDKRAAQPQMAHIDFGLSVLSRSLFDRYAPALVLDLADILHELSLR
                     GELAAFEVSERIYEIGSLQGLKDTEEFLSRQVSDP"
     gene            413..1012
                     /locus_tag="VI195_10475"
     CDS             413..1012
                     /locus_tag="VI195_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY6824855.1"
                     /translation="MSYAQQHLQETIAILQQLDLAAIEKVVGVVARLKAEGGRIFFLG
                     VGGSAGNCSHAVNDFRKLLGIECYAPTDNVAELTARINDEGWESAYAEWLKVSRLAAR
                     DAVFVLSVGGGSAEKNISRNLVAAVDYARTRGARVLGIVGRDGGHTARVADACVIIPT
                     VNPRSVTPHCEALQAVIWHLLVSHPRLGPGEAKWESLGS"
     gene            1136..1852
                     /locus_tag="VI195_10480"
     CDS             1136..1852
                     /locus_tag="VI195_10480"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY6824856.1"
                     /translation="MSALSQLRVKIFADGADRARMLELRAKPYIMGFTTNPTLMRRAG
                     VTDYRAFARGILAAITDKPVCFEVLADDFLDMERQAFEIASWAANVYVKIPITNTRRA
                     SSVDLVRRLAAGGVKLNVTALMTLEQLRAVLPALNANTPSYLSMFAGRIADTGIDPLP
                     LLAQAAEMLKAIPSAGLVWASSRELLNIFQAEAAGCHAITLTSDILSKLFLIGHDLAD
                     YSLETVRMFYDDAQSAGFTL"
     gene            complement(1875..>2862)
                     /locus_tag="VI195_10485"
     CDS             complement(1875..>2862)
                     /locus_tag="VI195_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824857.1"
                     /translation="GAFVKGTYDNIFDTKTWDNLDIRYARTVSLGGHSAVLGIDVNNN
                     PTVQDLWSSLPVFSFPYIRSELVPLPSVAPAIHFALGTTVLGGTIYSMIDNRFYAELG
                     FYKGVSNKWLGNLGEPGASPNIVGAAPYARFSVQEQKGAHYFRVGLVGFSVKQQPFTT
                     TSETNRSTDYGIDGSYQFNIGSPHAIDAHASWIHEKRQLDASFATGASDATSNSLDTF
                     EADVSYILRQTWSASAGLFDTNGTTNHLLFAPGAVFGSASGSPASRGYTLQLEWVPFG
                     KFGSFASPFVNLRLGLQYTGYWRFNGGSTNYDGFGRSASDNNTVFLYTWLAF"
BASE COUNT          515 a          887 c          931 g          529 t
ORIGIN      
        1 tcggcgagga tttcttcgtg ttgaacggcg actcctacct cccctgctcg ttgccgtcca
       61 ttcaggccgc ctaccgcgca gcgcggcgtc cggcactgat gaccatcatg cgcaacgaca
      121 accgctggga ccgcagcaac gtgctgttca gcgggggccg cctgcttgcc tacgacaagc
      181 gcgccgcgca acctcaaatg gcgcacatag acttcggcct gtcggtgctg tcacgcagcc
      241 tattcgaccg ctacgcgcct gctttggtcc tcgatctcgc ggacattctc cacgagctgt
      301 cgctgcgcgg ggagctggca gcgttcgaag tttccgagcg catctacgag atcggctcgc
      361 tgcagggcct caaggatacc gaggagttcc tctcgcggca ggtgagcgac ccatgagcta
      421 cgcgcagcag catctgcagg agaccatcgc catcctgcag cagctggacc tggcggcgat
      481 agagaaggtc gttggggtgg tcgcgcggct caaggcggag gggggacgca tcttctttct
      541 cggagtcggc ggtagcgccg gcaattgctc gcacgcggtg aatgactttc gcaagcttct
      601 cggcatcgag tgttacgccc cgaccgataa cgtcgcggag ctcactgcgc ggatcaacga
      661 tgaaggctgg gagagcgcct atgccgaatg gctcaaagtg agccggctcg ccgcccggga
      721 tgccgtgttc gtgctctcgg tcggtggcgg aagcgcggag aagaacatca gccgtaacct
      781 ggtcgcggcg gtcgactacg cccggacgcg cggtgcgcgg gtcctcggca tcgtcggccg
      841 ggacggcggc cataccgccc gtgtcgccga tgcgtgcgtc atcatcccca ccgtcaaccc
      901 tcgcagcgtg acgccgcatt gcgaggcgct gcaggcggtg atctggcatc tgttggtgtc
      961 tcatccccgg ctcgggccgg gggaggcgaa atgggagtcc ctcgggtcat gataggctgg
     1021 cgttcgcacg ctgccaccgc gccgggcgct cgtccggcgc tacggaggcg gtgaacgttc
     1081 caggggtgag cggctactca cgaacagccg ccaccgcgaa ccgtggggat agcacatgag
     1141 cgctttgagc cagctgcggg tgaaaatctt cgctgacggt gccgaccggg cccgcatgct
     1201 cgagctgcgg gccaagccgt acatcatggg cttcaccacc aatccgacgc tcatgcgcag
     1261 ggcgggtgtc accgattatc gcgcgttcgc gcggggcata ctcgcggcca taactgacaa
     1321 accggtgtgc ttcgaagtac tggcagatga tttcctcgac atggagcgcc aggcgttcga
     1381 gatcgcttcc tgggcggcca acgtgtacgt caagatcccg attaccaata cccggcgtgc
     1441 gagctccgtc gacctggtta gacggctcgc agccggtggt gtcaagctca acgtcaccgc
     1501 gctcatgaca ctcgagcagt tgcgcgcagt gctgccggcg ctcaatgcga acaccccgag
     1561 ctatctctcc atgtttgccg gccgcattgc cgacaccggc atcgatccgc tgccgctgct
     1621 ggcacaggcg gcggaaatgc tcaaggcgat tccgagtgcc ggactcgtgt gggccagctc
     1681 acgcgaactg ttgaacatct tccaggccga agcagccggc tgccacgcga tcaccctcac
     1741 cagcgacatc ctcagcaagc tcttcctgat cggacatgac ctggcggact actcgctcga
     1801 gacagtgaga atgttttacg acgatgcgca gagcgcgggc ttcacgctct agtcgtgcca
     1861 cgaccctgcg aacctcagaa cgcgagccag gtgtagagga acacggtgtt gttgtcgctg
     1921 gccgatcggc cgaagccatc gtagttcgtg ctaccgccgt tgaagcgcca atagccggtg
     1981 tactgcagcc cgagccgcaa attgacgaac gggctggcga aggagccgaa cttgccgaac
     2041 ggcacccact cgagctgcag ggtgtagccg cggctggccg gtgagccgga agcgctgccg
     2101 aagactgcac ccggtgcgaa cagcagatga ttggtcgtgc cgttggtgtc aaagaggcct
     2161 gctgaggcag accacgtctg ccgcaggatg tagctgacat ccgcctcgaa agtatcgaga
     2221 ctgttggacg tggcgtcgga ggcacccgtc gcgaagctgg cgtcgagttg ccgtttctcg
     2281 tggatccacg atgcatgcgc gtcgatcgcg tgcggcgatc cgatattgaa ctggtagctg
     2341 ccgtcgattc cgtagtcggt ggagcgattg gtctcggaag tggtggtgaa gggctgctgt
     2401 ttgacactga atcccaccag cccgacccgg aagtagtgtg ctcccttctg ctcctgcacg
     2461 ctgaagcgtg catagggggc tgcgccgaca atattggggc tggcgcccgg ctcgccgagg
     2521 ttgccgagcc acttgttgct cacgcctttg tagaagccga gctccgcgta gaaccggtta
     2581 tcgatcatcg agtagatggt gccaccgagc accgtagtgc cgagcgcgaa gtggatggcc
     2641 ggcgcaacgc tcggcaacgg taccagctcg gagcggatgt agggaaagct gaagaccggc
     2701 agcgagctcc acagatcctg cacggtgggg ttgttgttca catcgatccc gagcaccgcg
     2761 ctgtgtccac cgaggctcac ggtgcgcgcg taacgaatat cgaggttatc ccaggtcttg
     2821 gtatcgaata tgttgtcgta cgtgcccttg acgaacgcgc cc
//
LOCUS       DATDMP010000596         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_982315, whole genome shotgun sequence.
ACCESSION   DATDMP010000596 DATDMP010000000
VERSION     DATDMP010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982315"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VI195_10490"
     CDS             <1..384
                     /locus_tag="VI195_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824858.1"
                     /translation="GIAITLELDQSLGRLPRELEAAVFHIVQESLTNIHRHSGSKTAT
                     LRIARTGARLLLEIEDEGSGIPADKLREIQMQGSGVGIAGMRERVLHFNGDMRISSVS
                     TGTRIAVSFPVEPAGHRPEPLLLNS"
     gene            457..1149
                     /locus_tag="VI195_10495"
     CDS             457..1149
                     /locus_tag="VI195_10495"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEY6824859.1"
                     /translation="MTRHIGIVACSAEGAALCYRTVCLEGPQWLGPLAHPEVSMHTHS
                     FAAYMRCIESADWPGVAELMLAAAQKLARAGADFLICPDNTIHQAMPWVLRRSPLPWL
                     HIAEVVAAHAAERGFHRLGLTGTRYLVDGEVYPDKLTERGLEYLRPTPVERSEINRII
                     FEELVAGVFKPEAISFFQRVMSRMKDDGCDAIVLGCTEIPLIMNDGNSPLPTLDSTRL
                     LARAALQRATAP"
     gene            complement(1210..1422)
                     /locus_tag="VI195_10500"
     CDS             complement(1210..1422)
                     /locus_tag="VI195_10500"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY6824860.1"
                     /translation="MVESVYKIIELVGTSTESWEKAASAAVNQASKSLRDLRIAEIVQ
                     LDMHMQDGKIAAYRAKVKVSFKFERE"
     gene            complement(1534..>2466)
                     /gene="rocF"
                     /locus_tag="VI195_10505"
     CDS             complement(1534..>2466)
                     /gene="rocF"
                     /locus_tag="VI195_10505"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY6824861.1"
                     /translation="PTDVGAADRGASMGPEALRVAGLLAALRARDLEVEDLGNLSGPG
                     NPWQPPRDGYRHLPEVVRWNELVHESVYRELGDGRLPILLGGDHCLGIGSIGAVARYC
                     REQGRKLRVLWLDAHADFNTSELSPSGNVHGMPLACLCGFGPPELTGLGGRTPAIDPA
                     WVRQIGVRSVDAGEKRFVHEQGLDVFDMRHVDELGMRATMEQALSGMDSDTHLHVSFD
                     VDFLDPEIAPGVGTRVPGGPTYREAQLCMEMIADTRRLASLDVVELNPAFDVHNRTAT
                     LAVDLIESLFGKSTLVRRPVWDGPAKAPRSVSTA"
BASE COUNT          389 a          860 c          810 g          407 t
ORIGIN      
        1 ggcatcgcca tcacgctcga gctcgatcag agcctcggtc gcctgccgcg cgagctcgag
       61 gccgcggtgt tccacatcgt gcaggaaagc ctcaccaata ttcaccgcca ttccggcagc
      121 aagacggcca ccctgcgcat cgcgcgcacc ggcgcccggc tgctgctgga aatcgaggac
      181 gagggcagcg gtataccggc cgacaagttg cgcgagatcc agatgcaggg ctcgggtgtc
      241 ggcatcgccg gtatgcgcga gcgcgtgctg cacttcaacg gggatatgcg catcagctcg
      301 gtgagcaccg gcacgcgcat cgcggtgagc ttcccggtcg agcccgccgg ccatcgcccc
      361 gagccgctgc tgctcaattc ctgaggaacc gcggacgcag cccggcgcgt gtcactcgct
      421 gccaaagccg gctgcgattg acggtagaat ccggccatga cgcggcacat cggcatcgtc
      481 gcctgctccg ccgaaggcgc ggcgctctgt tatcgcacgg tgtgtctcga gggtccgcaa
      541 tggctcgggc cgctcgcgca ccccgaggtc tcgatgcaca cgcactcgtt cgccgcctac
      601 atgcgctgca tcgagagtgc tgactggccc ggggtcgccg agctgatgtt ggctgcggca
      661 caaaagctcg cgcgcgccgg ggcggacttc ctcatttgtc cggacaacac cattcaccag
      721 gcgatgccct gggtactgcg ccgctcgcca ctgccctggc tgcacatcgc cgaggtcgtg
      781 gccgcgcatg ccgccgagcg cggtttccat cgactcgggc tcaccggcac gcgctacctc
      841 gtcgacggcg aggtgtaccc ggataagctc accgagcgcg gactcgagta cctgcgcccc
      901 accccggtgg agcgctccga gatcaaccgc atcatcttcg aggagctcgt cgccggggtg
      961 ttcaagcccg aggcaatcag cttctttcag cgcgtgatga gccgcatgaa ggacgatggt
     1021 tgcgacgcca tcgtgctcgg ttgcaccgag atcccgttga tcatgaacga cggcaactcg
     1081 ccgctgccga cgctcgactc gacgcggttg ctggcgcgag ctgcgctgca gcgcgccacg
     1141 gcgccgtgaa cggccaactg agcgcgcccg cgcgccgggc gcgccgcagc cgctaccgtg
     1201 ctcgcacggc tattcgcgct cgaacttgaa ggagaccttc accttggcac gatacgctgc
     1261 gatcttgccg tcctgcatgt gcatgtcgag ctgtacgatc tcggcgatgc gcagatcacg
     1321 cagcgacttg gaagcctggt ttaccgctgc ggatgcagcc ttctcccagg actcggtgct
     1381 cgttcccacc agctcgatga ttttgtagac gctctcgacc atggcttgct caccttccgg
     1441 ccccgttgcg tgagggtcgc actgctattg ttggaatgat gcggcgagcc caggtcgatt
     1501 gcaacgcggc gccgagcgtg cgtcaggtgc agctcacgcc gtgctcaccg agcgcggcgc
     1561 tttcgccgga ccgtcccaca ccggccggcg caccagcgtg ctcttgccga acagcgactc
     1621 gatcagatcg accgcgagcg tcgcggtgcg attgtgcacg tcgaaggcag gattcagctc
     1681 gacgacatcg agcgaggcca gtcggcgcgt gtcggcgatc atctccatgc acagctgtgc
     1741 ctcgcggtag gtcggtcctc cgggtacgcg ggttccgact ccgggggcga tctccggatc
     1801 gaggaagtcc acatcgaagc tgacgtgcag gtgcgtatcg ctgtccatac cgctcagcgc
     1861 ctgctccatg gtcgcacgca tgccgagctc atcgacgtgg cgcatgtcga acacgtccag
     1921 gccctgctcg tgcacgaagc gcttctcgcc cgcatcgacg ctgcgtacgc cgatctgccg
     1981 cacccaggcc ggatcgatcg cgggcgtgcg accgccgagg ccggtgagct ccggcggccc
     2041 aaagccacag aggcaggcga gcggcatgcc gtgcacgttg ccgctcgggg agagctcgct
     2101 cgtgttgaag tccgcatgtg catcgagcca cagcacgcgc agcttgcggc cctgctcgcg
     2161 gcaatagcgc gcaaccgcgc cgatggagcc gatgccgagg cagtgatcgc cgccgagcag
     2221 gatcggcagc cggccgtcgc cgagctcgcg gtagacgctc tcgtgcacca gctcgttcca
     2281 gcgcaccact tcgggcagat gccggtagcc gtcgcgcggg ggctgccagg gattgcccgg
     2341 accgctcaga ttgccgaggt cctcgacctc gaggtcgcgc gcgcgcagcg cggcgagcag
     2401 tcccgccacg cgcagtgctt cgggccccat ggaggcgccg cggtcggcgg caccgacatc
     2461 ggtcgg
//
LOCUS       DATDMP010000597         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1760865, whole genome shotgun sequence.
ACCESSION   DATDMP010000597 DATDMP010000000
VERSION     DATDMP010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760865"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..773)
                     /locus_tag="VI195_10510"
     CDS             complement(114..773)
                     /locus_tag="VI195_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6824862.1"
                     /translation="MMIVKRLALLAAGLLVATCAFAQMRAGKSEFYLFPTFTTGQTYA
                     FEGGTTAQTDTGYGFGLGFAKNFTPNLAGGVEIVWGSSDYRSTVLPGPGNIGQPVSVR
                     GTVDSSTVRFHGTYYLGNADLAPFISGGLGWTYIDSNVPSGPPQGVCWWYPWWGQVCG
                     SYVPTATTTKFSYNVALGLRQDLRNGMFLRGLVNNQWVDIASSGTTSWTQWRIDIGWR
                     Y"
     gene            complement(770..1150)
                     /locus_tag="VI195_10515"
     CDS             complement(770..1150)
                     /locus_tag="VI195_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6824863.1"
                     /translation="MPYIAVVDDEEPVRKALGRLLRASGMEVECYASGREFLAGSAKR
                     RPDCVVLDLHMPGMSGLQVLAELRPKRPPLPAVVITAHDASEIRDQCFAAGATEYLRK
                     PLDERVLLDAIAASLRSAKTMENR"
     gene            complement(1193..>2005)
                     /locus_tag="VI195_10520"
     CDS             complement(1193..>2005)
                     /locus_tag="VI195_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6824864.1"
                     /translation="LFLTVVAVPLLLLAVALEERWRLERDSQAQRTQLAHLSRVSMLG
                     QLSGGIVHELNQPLTAILSNAQAAQHFIANKTSDPQLLAEILQDIIVADQRAGEVIRR
                     LHALFKRGETQFQQLDANELVQDVLRILHSDLVVRGVDVQTDLREDLPAIEGDRVELQ
                     QVLLNLVMNACDAMHAVEEGERRLQLRTRAGDPGGVTITVKDSGAGFDLEAYERLFEP
                     FYTTKPQGLGLGLSICRAIVRAHRGRLWGTAVPGRGATFHVLLPGRHAAMGP"
BASE COUNT          317 a          678 c          684 g          326 t
ORIGIN      
        1 tcgaagaact cggccggccc gcccttcagc atgttgacga actgcgcgct cgccagcggg
       61 ctggcgagcg cgagggcggc ggcaatcact agcttcattg cagcgttgct ggtctagtag
      121 cgccagccga tgtcaattcg ccattgcgtc cagctcgtcg tgcccgacga ggcgatgtcg
      181 acccactggt tgttcaccag cccgcgcagg aacatcccgt tgcgcaggtc ctggcgcagt
      241 ccgagcgcga cgttgtagct gaacttggtg gtggtcgccg tgggtacata gctgccgcaa
      301 acctggcccc accacgggta ccaccagcac acgccttgcg gcgggccgga cggcacgttg
      361 ctgtcgatat acgtccagcc cagaccgccg ctaatgaaag gcgccagatc cgcgttcccc
      421 aggtagtacg tgccgtggaa gcgaacggtg ctgctgtcga ccgtgccgcg cacgctgacc
      481 ggctggccga tgttgccggg cccgggcagc acggtggagc ggtaatcgga cgagccccac
      541 acgatctcga cgccacccgc caggttcggc gtgaagttct tcgcgaaccc caggccgaag
      601 ccgtagccgg tatcggtctg cgccgtggtg ccgccctcga acgcataggt ctggccggtc
      661 gtgaaggtcg ggaatagata aaactcgctc tttcccgcgc gcatctgcgc aaaggcgcac
      721 gtcgcgacca gcaggccggc ggcaagaagg gctaggcgtt tcacgatcat catcggttct
      781 ccatggtttt ggcgctgcgc aggcttgccg cgatcgcgtc gagcagcacg cgctcgtcta
      841 gcggcttgcg cagatattcg gttgcgccgg cggcgaagca ctggtcgcga atttccgacg
      901 cgtcgtgcgc cgtgatcacc acggcgggca ggggcggccg cttcgggcgc agctcggcga
      961 ggacctgcag gccgctcatg ccgggcatgt gcaggtcgag caccacgcag tcgggccggc
     1021 gcttcgccga tccggcgagg aattcgcggc cgcttgcata gcactcgact tccatccccg
     1081 aggcccgcag cagccggccg agcgccttgc gcaccggctc ctcatcatct acgacggcga
     1141 tgtacggcac ggtagcccat tggagcaggt cgtcccgcgc cgggtaattg gactaaggtc
     1201 ccatggccgc gtgccgtccg ggcaacagga cgtggaaggt cgccccccgt ccgggaaccg
     1261 ccgtgcccca tagccgcccg cgatgcgcgc gcacgatggc ccggcagatc gacaggccga
     1321 gccccagccc ttgcggcttg gtggtgtaga acggctcgaa caggcgctcg tacgcctcca
     1381 ggtcgaatcc cgcgccgctg tccttgaccg tgatcgtcac gccgccgggg tcgccggccc
     1441 gggtgcgaag ctggaggcgg cgttcgccct cctcgacggc gtgcatggca tcgcaggcgt
     1501 tcatgaccag gttcagcagc acctgctgca gctccacgcg gtcgccctcg atcgccggca
     1561 ggtcctcgcg caggtcggtc tggacgtcga cgccgcgcac caccaggtcg ctatgcagga
     1621 tgcgcagcac gtcctggacc agctcgttcg cgtcgagctg ctggaactgc gtttcgccgc
     1681 gcttgaaaag cgcgtgcagc cggcggatca cttcgccggc gcgctggtcg gcgacgatga
     1741 tgtcctgcag gatctccgcg agcagctgcg gatcggaagt cttgttggcg atgaagtgct
     1801 gcgccgcctg cgcattgctc aggatcgcgg tgagcggctg gttgagctcg tgcacgatgc
     1861 cgcccgagag ctggccgagc atcgagacgc gcgagagatg cgcgagctgc gtgcgctgcg
     1921 cctgcgaatc gcgctcgagg cgccagcgct cctcgagcgc gacggcgagg agcagcagcg
     1981 gcaccgccac caccgtgagg aagag
//
LOCUS       DATDMP010000598         2465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_872163, whole genome shotgun sequence.
ACCESSION   DATDMP010000598 DATDMP010000000
VERSION     DATDMP010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872163"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VI195_10525"
     CDS             <1..278
                     /locus_tag="VI195_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY6824865.1"
                     /translation="ALRGITRDTIFALARDLGIAVIERRITRDEVYCADEAFFTGTAA
                     EVTPIRELDNRQIGPGHRGPVTERLQTLFFDTVNGRNDRHRDWLTYV"
     gene            275..508
                     /locus_tag="VI195_10530"
     CDS             275..508
                     /locus_tag="VI195_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY6824866.1"
                     /translation="MSGPDASGRAMEASAPEAHPVVEVRSKDLPVFCPNPGMPLWSSH
                     PRVFLHFAENGEARCPYCGTRYRLAPGEAAPAH"
     gene            593..1630
                     /gene="waaF"
                     /locus_tag="VI195_10535"
     CDS             593..1630
                     /gene="waaF"
                     /locus_tag="VI195_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY6824867.1"
                     /translation="MTERILVVAPSWVGDAILSEPLIALLREPLGDPMVDVLAPPWCA
                     PVYARMRGIGRVIELPVGHGRVDLAARREIANKLRDEVGQPLYTRALVLPNSWKSALI
                     PWLAGIPRRTGYLGEARWVLLNDARRLRRKEMPRLVDRFAALAAQPGELVPMPPAPKL
                     VPDVGNRAAAVNALNLRSRKRVAILCAGAEYGPAKRWPPTHFAELAARFLESGMQVWL
                     VGSPNDRIAAEAVLRAAGELAPLIRDLTGHTDLGTAIDLLSLASVVVSNDSGLMHAAA
                     AVGAPLIALFGSSSPSFTPPLSPVAQIAKIEIACSPCFQRECPLGHFKCLRDLAPDAV
                     YDLARNAVRQS"
     gene            1688..2122
                     /locus_tag="VI195_10540"
     CDS             1688..2122
                     /locus_tag="VI195_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6824868.1"
                     /translation="MAKARTVPIYTTPTDAAVAFYQAFEAKDIDAMMSTWAEDEEIIC
                     VHPGGERMVGYDAVRAGWEQIFAGDARLSFRLEEVITIETVGLAMQSGIEQISLGKES
                     DPRGTAIATNVFMRTPSGWRMVMHHASPAPAASAGESKGPLH"
     gene            2151..>2465
                     /locus_tag="VI195_10545"
     CDS             2151..>2465
                     /locus_tag="VI195_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824869.1"
                     /translation="MDSARREHFDMMRVPEGSSPAIASRVARWLLHGPAQVASGPHAG
                     GVAGTLDADLTPRYAYPEDTGYYLQWLCWYATITGSATELVPRAVAAHRWLETWLALP
                     QPL"
BASE COUNT          359 a          864 c          816 g          426 t
ORIGIN      
        1 gtgcgctgcg tggcatcaca cgcgacacca tcttcgccct tgcgcgcgac ctcgggattg
       61 ccgtgatcga gcgccgtatc acgcgcgacg aggtgtactg cgcggacgaa gcgttcttca
      121 ccggcacggc cgccgaggtg acaccgattc gcgagctcga taaccggcag atcggccccg
      181 gccaccgcgg cccggtgacc gagcgcctgc agacgctgtt cttcgacacc gtgaacggcc
      241 gcaacgatcg tcaccgggac tggcttacct acgtatgagc ggccccgacg cctccggccg
      301 cgcgatggaa gcctctgcgc ctgaagcaca cccggtggtc gaggtgcgca gcaaggacct
      361 gccggtgttc tgtccgaatc ccggcatgcc gctgtggtcc tcgcatccac gcgtgttcct
      421 gcactttgcc gagaatggcg aagcccgctg tccctattgc ggcacgcgct atcgccttgc
      481 tccgggcgag gccgccccgg cgcactgacc gcgccaggtg ccgcgcgatc cctccctggc
      541 ggttctcgca cctccgaagc cgcggcgcgg gcttttcgct aatctccagg caatgaccga
      601 acgcatcctg gtggtcgcgc cctcctgggt cggcgacgcc atcctttcgg agcctctcat
      661 tgcgttgctg cgcgagccgc tcggcgatcc catggtggac gtgctcgcgc cgccctggtg
      721 cgcgccggtg tacgcgcgca tgcgcggtat cggtcgtgtc atcgagctgc cggtcggtca
      781 tggtcgcgtc gatctcgcgg cgcggcgcga gatcgcgaac aagctgcgcg acgaagtggg
      841 gcaaccgctg tacacgcgcg cgctcgtgct gccgaactcg tggaagtcgg cgctgattcc
      901 ctggctcgcc ggcatcccgc ggcgcactgg ctatctcggc gaagcacgat gggtgctgct
      961 caacgatgct cggcggttgc gtcgtaagga gatgccgcgc ctggtcgatc gatttgcggc
     1021 cctcgcggcg caaccgggcg agctcgtgcc catgccaccc gcaccgaagc tcgtgcccga
     1081 tgtgggtaat cgcgcggcgg ccgtgaacgc gttgaatctc cgctcgcgca agcgcgtggc
     1141 gattctgtgc gcgggcgcgg agtacggtcc cgccaagcgc tggccgccga cccatttcgc
     1201 ggagctcgcc gcccgtttcc tggaaagcgg aatgcaggtg tggctcgtcg gttcaccgaa
     1261 cgatcgaatc gccgccgagg ccgtgttgcg cgcggcgggc gagctcgcac cgctcattcg
     1321 ggatctcacc gggcacaccg atctcggcac ggcaatcgac cttctctccc tcgcctcggt
     1381 cgtggtcagc aacgattcgg gactgatgca cgccgcggcc gccgtgggcg cgccgctgat
     1441 cgcgctgttc gggtcgtcct cgccatcgtt cactccaccg ctgtcgcccg tcgcgcagat
     1501 cgcgaagatc gagatcgcgt gcagcccttg ctttcagcgc gaatgcccgc tcgggcattt
     1561 caagtgcctg cgcgatctgg cgccggacgc cgtgtatgat ctcgcgcgca acgcggtgcg
     1621 ccagagttga gtcgcgcgct cgcgtccctc cgattcctcg ccctattgct cggacattca
     1681 cggcagcatg gcaaaagcgc gcaccgttcc catctatacg acgcctaccg acgccgccgt
     1741 cgcgttctac caggccttcg aggcgaagga catcgacgcg atgatgagca cgtgggcgga
     1801 ggacgaggaa atcatctgcg tgcatcccgg cggcgagcgc atggtcggct acgacgcagt
     1861 gcgcgcgggc tgggagcaga tcttcgccgg cgatgcgcgg ctttcgttcc ggctcgaaga
     1921 ggtcatcacc atcgaaaccg tcggtctcgc gatgcaaagc ggcatcgagc agatctcgct
     1981 cggcaaggaa tcggatccac gcgggacggc gatcgccacc aatgtcttca tgcgcacgcc
     2041 ttccggctgg cgcatggtga tgcaccacgc gtcgccggcg ccggcggctt ccgctggcga
     2101 gagcaaaggt ccgctgcact gagcggcgcg gtccccgcgc acggcgcaat atggacagcg
     2161 cccggcgcga acatttcgac atgatgcgcg tgcccgaagg ctcctcgcct gcaatcgcgt
     2221 cgcgggtcgc gcgttggctg ctgcacggcc cggcgcaggt tgcgtccgga ccgcacgccg
     2281 gtggcgtggc cggaacgctc gacgcggatc tgacgccgcg ttacgcctat cccgaggaca
     2341 cgggctatta cctgcaatgg ctttgctggt acgcgacgat caccggcagc gccactgagc
     2401 tcgtgccgcg cgcagtggcc gcgcatcgct ggctcgagac gtggctcgcg ttgccgcagc
     2461 ccctc
//
LOCUS       DATDMP010000599         4737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1761182, whole genome shotgun sequence.
ACCESSION   DATDMP010000599 DATDMP010000000
VERSION     DATDMP010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761182"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VI195_10550"
     CDS             complement(<1..1004)
                     /locus_tag="VI195_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824870.1"
                     /translation="MTLKDHRDLEISGATPAARDAFERALEAHLGWRSGADRYLEQVV
                     QAAPTFTMAHVLRGYLNLCSRDQAQVRQAQVAYVSASTLPATARERLHVAAMGAVLAD
                     DFESFQSIVQDLLEQYPRDVLALQIGHALDYLTGDIEAMAERISSVLPAWSQDTPGYH
                     AVLAMQAFSLVESAQYGRAADCGLRALELDPWDTRAHHALTHVYEMTGSATAGMRWMQ
                     RRRIYWATDTVAATHLWWHWALFHLARGEIQVALTVYDEHVRSSRSAHIADMIDASAL
                     LWRLELLGGDIGGRWTELAAAWEARIGDGYCTFNDVHAMLALIGARNWRGAGRLESAL
                     "
     gene            complement(1017..1862)
                     /locus_tag="VI195_10555"
     CDS             complement(1017..1862)
                     /locus_tag="VI195_10555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6824871.1"
                     /translation="MNAPIPALQPPPCEVIDFDAIKRRQQATWSSGDFAIIGTTLQIV
                     GESLAEAADVRAGERVLDVAAGNGNASLAAARRFARVTSTDYVPQLLEKGAARARAEG
                     VEISFQVADAENLPFADGEFDVVLSTFGAMFTPEHHRAAQELLRVTRSGGRIGLANWT
                     PEGFLGELFGVVGRFVAPPPGTRSPTLWGSEPYIVGLFGPQARELRCVRRHFNFRYRS
                     AAHWIEVFRAFYGPTHKAFAALDAARQSQLSAALMELLGRWNVAGRDSLVVPGEYLEI
                     VVTKA"
     gene            2045..3094
                     /locus_tag="VI195_10560"
     CDS             2045..3094
                     /locus_tag="VI195_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6824872.1"
                     /translation="MTRDTLSDLLRSVRLRGAAFYYVSFRGQWSAGAAAAAEVAPLVM
                     PGCDHVMEYHMFAKGNGWAAVDGLPPVRLQAGDVVLFPQGDRHVVSSAPGLTPSRERP
                     ELLLARSAQRPLPISFQEGIVAAGPMPIESADAVVVCGFLGCDVRPFNPLITALPRIL
                     HLPASRAGGWVAHAIDQAVLESNERRPGGEAVLERLSEMMFVDAARRYLESLPEDSVG
                     WLAGLRERHVGRALALMHEQPEREWSIDDLAREVGLSRSALHERFVRYVGQAPMQYLA
                     SWRMQLGARLLRESTRLVAEIALEVGYDSEAAFARAFKRMVGMPPAAWRRAQARGLRS
                     EAASASPAALQPPQP"
     gene            3261..3662
                     /locus_tag="VI195_10565"
     CDS             3261..3662
                     /locus_tag="VI195_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY6824873.1"
                     /translation="MSNSSALADFATRYTAAWCSHDAARVASFYSESGALSINDGTPA
                     VGRKAVEAAAQSFMTAYPDLIVKFDRLEPRGARVRYHWSFIGTNTGTGGTGNPVRISG
                     FEDWKIGPDGLSADSKGHYDAREWDRQVNRR"
     gene            complement(3689..4705)
                     /locus_tag="VI195_10570"
     CDS             complement(3689..4705)
                     /locus_tag="VI195_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6824874.1"
                     /translation="MPNESPSGQRDQAVLVTGSAGHLGEAVMRYLRAAGRSAVGLDLK
                     ASAFTNHVGSICDKGFVARCMQHVSAVVHAATLHKPHVATHSWQDFVEVNVTGTLHLL
                     EAAVAAGVRSFIYVSTTSTFGEALYPAAAEPAIWVTETLIPRPKNIYGSTKLMAEGLC
                     ELAHRQRQLPIMILRTSRFFPEPDDAADIRHQYQVANAQANELLYRRVDIEDVVSALV
                     SAIDRAPRIGFCRYIISATTPFSPNDLELLRRDAAQVVGRLFPEYVALYAARGWTMFP
                     RIDRVYVNERARTALEWQPKYDFAHVLDCLRANRDFHSALAREVGSKGYHDRDFGERP
                     YPVD"
BASE COUNT          835 a         1514 c         1544 g          844 t
ORIGIN      
        1 tgcaatgcgc tttccagtcg cccggcgccg cgccaattcc tcgccccgat tagcgcgagc
       61 atcgcgtgca cgtcgttgaa ggtgcagtag ccgtctccga tacgcgcctc ccaggccgcg
      121 gccagttcgg tccagcgtcc gccgatgtcg ccccccagca actcgagccg ccacaggagc
      181 gccgacgcat cgatcatgtc ggcgatgtga gcggaacggc tgctgcgtac gtgctcatcg
      241 taaaccgtga gcgccacctg gatctcgccg cgtgcgagat gaaacaacgc ccagtgccac
      301 cagaggtgtg tggcagcgac cgtatcagta gcccagtaga tgcgacgccg ctgcatccag
      361 cgcattcccg cggtggcact gccggtcatc tcatatacat gtgtcagcgc atggtgcgct
      421 cgggtatccc aggggtcgag ttccaacgcg cgcagcccgc agtcggcggc acggccgtac
      481 tgcgcagact ccacgaggct gaaggcctgc atcgcgagca cggcgtggta accgggcgtg
      541 tcctgcgacc atgccggcag gacactgctt atacgttcgg ccatggcctc gatgtcgccg
      601 gtcaggtaat cgagcgcatg gccgatctgc agtgcgagca catcccgcgg atactgctcg
      661 agcaggtcct gcacgatgct ctggaacgac tcaaaatcat cggcaagcac cgctcccatg
      721 gcggccacgt gaagccgctc gcgcgcggtg gccggcagcg tcgaagcgct cacataggca
      781 acctgcgcct ggcgtacctg cgcctggtcg cgactgcata gattgaggta accgcgcagc
      841 acgtgcgcca tggtgaaagt tggcgcggcc tgcaccacct gctccaggta ccggtcggcg
      901 ccgctacgcc agccgaggtg cgcttcgagg gcgcgttcaa acgcatcccg ggcggcgggt
      961 gtcgcccccg agatctccag atcacgatga tccttcaagg tcatgtcagt agcgcctcag
     1021 gccttggtca ccacgatctc gagatactct ccgggtacca ccagcgagtc ccggccagcc
     1081 acattccacc gcccgaggag ctccataagc gctgcgctca gttgcgactg ccgcgcggcg
     1141 tcgagcgcgg cgaacgcctt gtgcgttggc ccatagaagg cgcggaacac ctcgatccaa
     1201 tgcgcagctg agcgatagcg aaaattgaag tgccggcgca cgcagcgcag ctccctcgcc
     1261 tgtggtccga acagccctac gatgtacggc tcggagcccc acagcgtggg cgagcgggtg
     1321 ccggggggcg gagcgacgaa gcggccgacg acaccgaaca gctcgccgag gaaaccttct
     1381 ggagtccagt ttgccagccc gatacgtccg ccgctgcgcg tcacgcgcag caattcctgc
     1441 gcggcccgat gatgttcggg cgtgaacatc gcaccgaacg tggacagcac aacatcgaat
     1501 tctccatcgg cgaacggcag gttctccgca tcggcgacct ggaacgaaat ctccacgcct
     1561 tcggcacgcg cgcgcgccgc gcccttctcc agcagctgtg gcacatagtc ggtggaagtc
     1621 acgcgcgcaa agcggcgggc ggctgcgagt gaggcattgc cgttgcctgc ggcgacatcc
     1681 agcacccgct cgcctgcgcg cacgtcggcg gcctcggcga gcgactcgcc gacgatctgc
     1741 agggtggtgc cgatgatcgc aaagtcgccg ctcgaccagg tggcctgctg gcgtcgcttg
     1801 atagcatcga aatcgatgac ctcacacggt ggtggctgca gtgctgggat tggtgcattc
     1861 actgatctct cctcgcgtcc tgggtggtgc acagattcaa ctgcggtgcg agtaagtttg
     1921 gcgcttggta tcgattgacg cttcaccgct cgtcttgcag acttgcctgc gcatccgcat
     1981 cgcgcggcgg cttgatccag aatccgcgcg ccttgatcca gcgtccaccg atcttgaagc
     2041 ctcgatgact cgcgataccc tgtcagatct gttacgaagc gtgcgcctgc gcggcgccgc
     2101 gttttattac gtcagtttcc gcgggcaatg gtcggccggc gcggcggccg ccgcagaggt
     2161 cgcgccgctg gtgatgcccg ggtgcgacca cgtcatggaa taccacatgt tcgccaaggg
     2221 aaacggttgg gctgccgtcg acgggttgcc accggtgcgt ttgcaggccg gcgatgtggt
     2281 cctattcccg caaggcgatc gacatgtggt ctcgagtgcc ccgggcctga cgccgagcag
     2341 ggagcggccg gagctgctgt tggcccggag cgcgcagcgg cccttgccga tctcttttca
     2401 agaaggcatt gtcgctgcgg gccccatgcc gatcgagagc gccgacgcgg tcgtggtatg
     2461 cgggtttctc ggttgcgatg tgcggccctt caatccactg atcacagcgc tgccgcgcat
     2521 cctgcatctg ccggcatcgc gcgccggcgg ctgggtcgct cacgccatcg atcaggcggt
     2581 gctggagagc aacgagcgcc ggccgggggg tgaggccgtt ctcgagcgcc tgtcggagat
     2641 gatgttcgtc gacgcagcgc ggcgctacct cgagagcctt cccgaggact cggtcggctg
     2701 gctcgcagga ctgcgcgagc gccatgtggg tagggcgttg gcattgatgc atgagcagcc
     2761 ggagcgcgag tggagcatcg atgacctggc gcgcgaggtc ggtctgtcgc gatcagcatt
     2821 gcacgagcgc ttcgtgcgtt atgtcgggca ggcgccgatg cagtacctcg ccagttggcg
     2881 catgcagctt ggtgcgagac tcttgcgcga atccacccgt ctggtcgccg agatcgcact
     2941 ggaggtcggt tatgactccg aagcggcgtt tgcgcgcgcc ttcaagcgaa tggtcggcat
     3001 gccaccggcc gcgtggcgcc gggcccaggc gcgcggcctg cggtccgaag cggcgagtgc
     3061 ctcgccggcc gcattgcaac caccgcagcc gtgaaccggc ccgccggcaa acctgcgcct
     3121 ggctactggc gcggctattg acgccgagcg cccggtggcc ctgactgcca atgagaatac
     3181 ccctgcggcc cacactactg acggcgagta tcgcgcttgg cttcgctgct ctgggcgcat
     3241 gtgggcagga gagccaatcc atgagcaact catcagcttt ggcagatttt gcaacgcgct
     3301 acacagctgc gtggtgcagc catgatgccg cgcgggtcgc gagcttctac tcagagtcgg
     3361 gggcgctgtc gatcaacgat ggaacgcctg cggtaggtcg caaagccgtc gaggctgccg
     3421 cgcaaagctt catgacggct tacccagacc tcatcgtgaa gttcgatcgc ctcgagcccc
     3481 gcggcgctcg agtgcggtat cactggagct tcatcggaac gaacaccggg acaggcggca
     3541 ccggcaatcc agtccgcatc agcggattcg aagattggaa gattgggccg gacgggctga
     3601 gtgcggattc gaaagggcac tacgatgccc gcgagtggga tcgacaagta aaccgccggt
     3661 gaggtcttct caggaagcgg cgtgacagct agtcgaccgg gtaagggcgt tcgccgaaat
     3721 cacgatcgtg ataaccctta ctcccaactt cccgtgctag cgcgctgtgg aagtcgcgat
     3781 ttgcccgcaa gcagtcgagc acgtgcgcaa agtcgtactt tggctgccat tccagagccg
     3841 ttctggcgcg ctcattgacg taaactcggt cgatccgtgg aaacatcgtc cacccacgcg
     3901 ccgcgtagag cgccacatac tcggggaaga gccggcctac aacctgcgcc gcatccctac
     3961 gcagtagctc cagatcattc ggtgagaagg gagttgttgc cgagatgatg tagcggcaaa
     4021 aaccgatgcg cggagctctg tcgatggccg agacaagcgc gctaacgaca tcttcgatat
     4081 cgacccgcct gtacagcaat tcgttggcct gggcgtttgc aacctggtac tggtgccgga
     4141 tatcggcggc atcgtccggc tccgggaaga agcgcgaggt gcgcaatatc atgatcggca
     4201 gctgccgttg cctgtgcgcc agctcgcata ggccctccgc catgagcttg gttgatccgt
     4261 agatattctt cggccgcggg atgagtgtct cggtcaccca gatcgcgggc tccgcagctg
     4321 ccgggtacag cgcctcaccg aaggtgctgg tggtgctgac gtagatgaag gaacggacgc
     4381 cagccgctac cgctgcttcg agcagatgaa gcgtgcccgt gacgttgact tcgacgaagt
     4441 cttgccagga gtgagttgca acatgtggct tgtgcagggt ggccgcatga acaaccgcac
     4501 tgacgtgctg catgcagcgc gcgacgaagc ccttatcgca aatggagcca acgtggttgg
     4561 taaaggcaga cgctttgagg tcgagcccca cggcggaccg gcccgctgcc cgcagatagc
     4621 gcatgaccgc ctctcccaag tgtccggcac tgccggtcac caggacggcc tggtcccgct
     4681 ggcccgacgg cgactcgtta ggcatacatg cagcgctcgt gcgagtctat tggcgtt
//
LOCUS       DATDMP010000600         4117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2094967, whole genome shotgun sequence.
ACCESSION   DATDMP010000600 DATDMP010000000
VERSION     DATDMP010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094967"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VI195_10575"
     CDS             <1..483
                     /locus_tag="VI195_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824875.1"
                     /translation="GRSTQLAQSSLRFSLGRPTREDDVDCAAAAVKEALARLRELSPA
                     APARQRTASGPALAGTGTAAALVTGEAGGPGEETWVRFSLLTAGDTVKEARFQAFACP
                     HTMQVAAWLCGALRGRTRQMLIPGTPGAWAQEHQVPPEKLGRLLVVEDALRACLASWT
                     "
     gene            586..909
                     /gene="iscA"
                     /locus_tag="VI195_10580"
     CDS             586..909
                     /gene="iscA"
                     /locus_tag="VI195_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY6824876.1"
                     /translation="MSISLTESAANRVKSFLAARGHGTGLRLGVRKTGCSGFAYVINY
                     ADGPQPGDAIFEDRGVRVFVDAESLTLIDGTVVDFVKQGLNEAFRFRNPNVKGECGCG
                     ESFSV"
     gene            1195..1623
                     /gene="ndk"
                     /locus_tag="VI195_10585"
     CDS             1195..1623
                     /gene="ndk"
                     /locus_tag="VI195_10585"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY6824877.1"
                     /translation="MALERTLSIVKPDGVKANQIGEVYRRFESAGLSIVAARMLQLSQ
                     REAEALYAVHRERAFFRDLVRYMTSGPVMVQALEGENAIARNRDIMGATDPKKAAAGT
                     IRADLASSIEQNVVHGSDAADTAAREIAFFFSTTELHSRG"
     gene            1643..2797
                     /gene="rlmN"
                     /locus_tag="VI195_10590"
     CDS             1643..2797
                     /gene="rlmN"
                     /locus_tag="VI195_10590"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY6824878.1"
                     /translation="MTTSGALRTNLLGLPREELVEFVAQLGGQPFRARQLMNWMYKRG
                     TGAFAAMSDLAKDFRARLALAAEVRAPDILRVQRSADGTCKWLLQADANQAFEMVFIP
                     EPDRGTLCISSQVGCALDCTFCSTAQQGYNRNLTTAEIVGQVWLAARELAREELAGVA
                     AAVQPGQGQRDERHVTNVVLMGMGEPLANFRNVVPALRIFLDDFGFDLSRRRVTLSTS
                     GLVPQIYRLAEETNVALAVSLHAPDDALRNELVPINRKHPIGELLQACWHYLDEQNGR
                     SVTFEYVMLDGVNDSPPQARALAQLLKGHPAKMNLIPFNPFPGTHYRRSPAHVIQRFR
                     DELLQRGLLATIRRTRGDDIDAACGQLAGRVIDRTTVRLGNKVIGVAVQP"
     gene            2794..3588
                     /gene="pilW"
                     /locus_tag="VI195_10595"
     CDS             2794..3588
                     /gene="pilW"
                     /locus_tag="VI195_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY6824879.1"
                     /translation="MSKHSNLPRRAQLRARPICASAAALLLAACAGHTPQQTDKAHEA
                     AIYNVQLGVAYMNQGDLERAKEKLERALAQDPNSADVRSARAALFARLGEKDKADSEY
                     RAALRIAPHDPRMVNNYAVYLCQNGRADEGVKRFLEAAHNPLYPTPEAAYTNAGVCLR
                     SAKRDDEARINFMRALQLKPNFAEAQFQLANLQFDHGELVPARNGIDGYMGTYNATPD
                     LLLLGVRVARAQGDRVAAQRYARKLQLDFPGTDQARALAALDHNPG"
     gene            3594..>4117
                     /locus_tag="VI195_10600"
     CDS             3594..>4117
                     /locus_tag="VI195_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6824880.1"
                     /translation="MTAEAGGSERRGIGARLRAAREQRGLTLLQAAERLHVDGRILQA
                     LETEDFAALGADVYVRGHLRRYAEAVGESAPQLQELYAEGPQPSRPDLTRIPRGEPEN
                     RGSRLLLPAVLLTVGLAVVGLLWWLLTLPGEKAHLLPADRAAATAPLTETPDADVPAP
                     AAADANAARSGSAPA"
BASE COUNT          690 a         1396 c         1384 g          647 t
ORIGIN      
        1 ggccgcagta cccagctcgc gcagagctcg ctgcgcttca gcctcgggcg tcctacccgc
       61 gaggacgacg tggattgcgc ggccgcggcg gtgaaggagg cgctcgcgcg gctgcgggag
      121 ctctcgcccg cggctcctgc gcggcagagg actgcaagtg gtccggcgct tgccggcact
      181 ggaaccgcag cagcgctcgt gaccggggaa gcggggggtc cgggggagga gacctgggtg
      241 cgattttctc tgctcaccgc gggggacacc gtgaaagagg cacgttttca ggcttttgca
      301 tgcccccata ctatgcaggt ggccgcgtgg ctctgtgggg cactgcgcgg gcgaacgcgc
      361 cagatgctga ttccgggaac accgggcgca tgggcacagg agcaccaggt gccgcccgag
      421 aagcttggcc gactgctcgt cgtggaagat gccctgcgcg catgcctcgc gagctggact
      481 tgaggcggtg tggaccccgc caaaaccgcg cctcccgcga tccggtggcc ccccgcagcg
      541 agcagcgcgc gcgatttccc agcctgcctt aaaatacgag gagcgatgag catttcactg
      601 actgaatccg cagccaaccg ggtcaagagt ttcttagcgg cacgcgggca cggcacgggc
      661 ctgcggctcg gggtgcgcaa gaccggctgc tccggctttg cctacgtgat caattacgcc
      721 gatggcccac agccggggga tgcgatcttc gaggatcggg gagtacgggt gttcgtcgat
      781 gccgaaagtc tcacgctgat cgacgggacg gtcgtcgact tcgtgaagca ggggctgaac
      841 gaggcttttc ggttcaggaa tccgaacgtc aagggcgaat gcggttgcgg ggagagcttc
      901 tccgtctgag ccggcggccg gccaagctcc tgggttttcg ctccggaggc cgcggcggcc
      961 caaaaactct ttaaaagacg cacacttcca gtgtgcgcca aattgccgca ggctcggccg
     1021 ccaaggtaaa ctgcgccgct tgcccggggt ccccgcgaaa ttgcgcgtct tgctggaccg
     1081 gtgcggggtc ggccctaacg gcaagcgcaa ttttcgtggg gtggtgtacc gcggggtggc
     1141 gcgtccgccg ccggaattct cccactcgtc tgctcagcac aaaggtcaca gcgaatggcg
     1201 ctagagcgca ccctgtccat cgtcaagccc gacggcgtca aagccaatca gatcggcgag
     1261 gtatatcggc gtttcgagag cgccggtctt tccatcgttg ccgcccgcat gctgcagctc
     1321 tcgcagcgcg aggccgaggc tctctacgcg gttcaccgcg agcgtgcctt cttcagggac
     1381 ctcgtgcgtt acatgacttc gggtccggtc atggtgcagg cgctggaggg tgagaacgcc
     1441 atcgcgcgca accgcgacat catgggcgcg accgatccga aaaaggccgc cgcaggcacc
     1501 atccgcgcgg atctggcgag cagcatcgag cagaacgtcg tgcacggctc cgatgcggcc
     1561 gacaccgcgg cgcgcgagat tgccttcttc ttcagcacga ccgagctgca ctcgcgcggc
     1621 tgaagcccgc aagccgcagg ccatgaccac ttccggcgcg ctacgcacca acctcctcgg
     1681 actcccccgt gaggagctcg tggagttcgt ggcgcagctg ggcggccagc ccttccgggc
     1741 gcgccagctc atgaactgga tgtacaagcg tggcacgggc gctttcgccg ccatgagcga
     1801 cctcgccaag gacttccgtg cccgtcttgc gctcgccgcc gaggtgcgcg caccggatat
     1861 ccttcgcgtg cagcgctcgg cggacgggac ttgcaaatgg ttgctgcagg ccgatgcgaa
     1921 ccaggcgttc gagatggtgt tcattcccga gcccgaccgc ggcacgctgt gcatctcctc
     1981 gcaggttggc tgtgcactcg actgcacctt ctgctccacc gcgcagcagg gctacaaccg
     2041 caacctcacc accgccgaga tcgtcggcca ggtctggctc gcagcgcgcg agctggcgcg
     2101 ggaagagctc gccggggtcg cggcagctgt gcagccgggg cagggtcagc gcgacgagcg
     2161 gcacgtgacc aacgtcgtgc tgatgggcat gggtgagcca ctcgccaact tccgcaacgt
     2221 ggtgccggcg ctgcgcattt ttctcgacga cttcggattc gacctgtcgc ggcgccgggt
     2281 gacgctgtcg acctcggggc tggtgccgca gatctaccgg ctcgcagagg aaaccaacgt
     2341 cgcgctggcg gtgtcgttgc atgcgcccga tgatgcgctg cgtaatgagc tcgtgccgat
     2401 caaccgcaag catcccatcg gtgagctgct gcaggcctgc tggcattacc tcgatgagca
     2461 aaacggccgc agcgtcacct tcgagtacgt catgctggat ggcgtcaacg actcgccgcc
     2521 ccaggcgcgc gcgctcgcgc agctactcaa gggacacccg gccaaaatga atctgatccc
     2581 gttcaatccg ttccccggca cgcactaccg gcgctcgccc gcccacgtaa tccagcgctt
     2641 ccgagatgag ctgctgcagc gcgggctgct cgccaccatc cgccgtacgc gcggcgatga
     2701 catcgatgcc gcctgcggac agctcgccgg ccgggtcatc gatcgcacca ccgtacggct
     2761 cggcaacaaa gtcatcggcg tcgcggttca accatgagca aacacagtaa ccttccccgt
     2821 cgcgcgcagc tgcgcgcgcg gccgatctgc gccagcgcag cggcgctgct gctcgccgcc
     2881 tgcgccggcc acacgccgca gcaaaccgac aaggcgcacg aggcggccat ctacaacgtg
     2941 cagctgggcg tcgcctacat gaatcagggc gacctcgagc gcgcgaagga aaagctcgag
     3001 cgcgcgctcg cgcaggatcc gaacagcgcc gatgtgcgca gcgcccgtgc cgcgctgttc
     3061 gcgcgcctcg gcgagaagga caaggcggac tcggaatatc gcgccgcatt gcgcatcgca
     3121 ccacacgatc cgcgcatggt gaacaactac gccgtgtacc tctgccagaa cggccgcgcg
     3181 gatgagggcg tgaagcgctt cctcgaggcg gcgcacaacc cgctgtaccc tactccggaa
     3241 gccgcttata ccaacgcggg ggtctgcctg cgctccgcca agcgcgacga tgaggcacgg
     3301 atcaatttca tgcgcgcact gcaactgaag ccgaacttcg cggaagcgca gttccagctc
     3361 gccaacctgc agttcgacca cggcgagctc gtgccggcgc gtaacggcat cgacggctac
     3421 atgggtacct acaatgcgac gccggatctg ctgctgctcg gggtgcgcgt cgcccgtgcc
     3481 cagggtgatc gggtcgccgc acagcgctat gcccgcaagc tgcagctgga ttttcccggc
     3541 accgatcagg cgcgcgcgct ggcggcactc gatcacaacc cgggctgatc cgcatgacgg
     3601 ctgaagcggg cggcagtgag cggcgcggta tcggcgcgcg gctccgcgcc gcgcgcgagc
     3661 agcgtggact cacgctgttg caggcggcgg aacggctgca cgtcgacggg cgcatcctgc
     3721 aggcactcga gaccgaggac ttcgccgcgc tcggcgcaga tgtgtacgtg cgcgggcacc
     3781 tgcggcgcta cgccgaagcg gttggagagt cggcaccgca gctgcaggag ctgtatgcag
     3841 agggcccgca gccctcgcgt cccgatctca cccgcatccc gcgcggcgag cccgagaacc
     3901 gcgggtcgag attgctgctg ccggccgtgt tgcttacggt cgggctggcg gtggtgggac
     3961 tgctgtggtg gctgctcacg ctgcccggag agaaggcaca tctgttgccc gccgaccggg
     4021 cagccgcaac ggcacccctc accgagacgc cggacgccga tgtgccggca cccgccgctg
     4081 cggacgcgaa cgcagcgcga agcggctcgg ctcctgc
//
LOCUS       DATDMP010000601         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_317186, whole genome shotgun sequence.
ACCESSION   DATDMP010000601 DATDMP010000000
VERSION     DATDMP010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317186"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="VI195_10605"
     CDS             <1..1873
                     /locus_tag="VI195_10605"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY6824881.1"
                     /translation="GIALLLIAWRLLTAHRGGGGDPGVTARALLALALVAIVLARFHT
                     LNGLAAGTTLLLLMAGLKLLETRAARDQFIMVSAGLFLLLAACLDRQELVRAPLYGLQ
                     AWLSCAALAMVAAPSTSARAAISLAGRALLLALPLALLLFVFFPRLSGAFWAIPRNDL
                     ALSGLSDTMTPGSIGQLATSYDPAFRVQFLGPVPPPQERYWRGPVLHEFDGQSWRRNA
                     FELRAPVPRQYLGTAYRYRVQLEPSRQRWWFALDTPTAAPDARARLTYDDQIVGAEPL
                     TDPVSFEVVSYTQTRGTQPLEAAAQRQDTALPPAGNPRTRALAESLRAHAGSDVALVQ
                     AALGYLRDGGFAYSLEPERLGPDAIDDFLFRTRVGFCGHYASAFVVLMRAAGVPARVV
                     TGYLGGEWIPYGGYLLVRQSEAHAWAEVWLQGRGWARVDPTAVVAPERLQRGILDLMP
                     QELSVRGRLLHASPWLAQWLQRWDAANAWWTNHVVKFNYDTQLSMLERLGIRSPDARD
                     LGWAFVLALCGWLAVIAWHVGRSMRPTRPDPLARAYARLCRKLAHVVPRAPHQGPLDF
                     AAALSARRPQVQAAVLPLLTRYAELRYGPPTPQTRAQDIRAFREAVRRLSLPRGEIR"
     gene            2056..2502
                     /locus_tag="VI195_10610"
     CDS             2056..2502
                     /locus_tag="VI195_10610"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY6824882.1"
                     /translation="MDRLGPVQTGIDARRRSGGFTLIELMITVAIVAILAAIALPSYS
                     IFMKRSRRGDTESSMMDIAQREQQYLLDTRTYAPNVAALNTTVPADVTAYYTIQICQT
                     AVPCAAPGGAPPTFVVIATPIAGTAQAGDPTLTLDNTGVKGPAGVW"
     gene            2520..3020
                     /locus_tag="VI195_10615"
     CDS             2520..3020
                     /locus_tag="VI195_10615"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY6824883.1"
                     /translation="MRASCHNCYGSAASARRRQRGFTLVEAIVTVSIAAILATVAVPA
                     FNGIIANQRAKTLASALYATLSKTRSEALTLNGNVTLQPKAGGWGNGWQMLDPNNNVL
                     DDYTTTSGITIAGPAAVTYRASGRLPTGAAPMFQISTMSGATVNYQCVSVDLGGRPYM
                     SPSQTC"
BASE COUNT          462 a         1139 c          999 g          527 t
ORIGIN      
        1 ggggatcgcg ctgctcctca tcgcctggcg gctgctcacc gcgcaccgcg gcggcggcgg
       61 cgatcccggc gtcacggcgc gcgcgctgct cgcactcgca ctggtggcga tcgtgctcgc
      121 gcgctttcat accctcaacg ggctcgcggc cggcacgacg ctgctgctgc tgatggcggg
      181 gctgaagctg ctcgagacac gcgccgcgcg cgatcaattc atcatggtgt cggcgggcct
      241 gttcctgctg ctggcggcct gcctcgaccg ccaggagctc gtgcgcgcgc cgctgtacgg
      301 tttgcaggcg tggctgtcct gcgccgcgct cgccatggtc gcggcgccat cgacttccgc
      361 gcgtgccgcg atttccctcg cgggccgggc cctgctgctc gcgctgccgc tcgctctgct
      421 gctgttcgta ttctttccgc gactgtcggg ggcgttctgg gcgattccgc gcaacgatct
      481 cgcactgagc ggcctgtccg acaccatgac cccggggagc atcggacagc tcgcaaccag
      541 ctacgatccc gccttccgcg tgcagttcct cgggcccgtg ccaccgccgc aggagcgtta
      601 ctggcgcgga cccgtgctgc acgagttcga tggtcagagc tggcggcgca acgccttcga
      661 gctgcgcgcg cccgtaccgc gccagtacct cggcaccgcc tatcgctacc gcgtgcagct
      721 cgaacccagc cgccaacgct ggtggtttgc gctcgacacg ccgaccgcgg ctccggacgc
      781 gcgcgcgcgg ctcacctacg acgatcagat cgtcggcgcc gagccgctca ccgacccagt
      841 gagcttcgaa gtggtgtcgt acacacagac ccgcggcaca cagccgctgg aggcggcggc
      901 gcagcgccag gacacggcac tgccgccggc gggcaatccg cgcacgcgcg ccctcgccga
      961 gagcctgcgc gcgcacgccg gctccgacgt cgcgctggtg caggcggcgc tcggctatct
     1021 gcgcgacggc ggtttcgcat actcactcga gccggagcgg ctcggccccg atgccatcga
     1081 tgattttctc ttcaggacac gcgtcggctt ctgcgggcat tacgcctcgg ccttcgtggt
     1141 gctgatgcgc gccgcgggtg tcccggcgcg ggtagtgacc ggttatctcg gaggtgagtg
     1201 gataccctac ggcggctatc tcctcgtgcg ccagtcagag gctcatgcat gggcggaagt
     1261 gtggctccag ggccgcggct gggcgcgcgt cgatccaacc gccgtggtcg cgcccgagcg
     1321 gctgcaacgc ggcattctcg atctcatgcc gcaggagctg tcggtgcgcg gccgcctgct
     1381 gcacgcctcg ccctggctcg cgcagtggct gcagcgttgg gacgcggcca acgcctggtg
     1441 gaccaaccac gtggtgaaat tcaattacga cacccagctc agtatgctgg agcgtctcgg
     1501 catccgctcg cccgatgcac gcgatctcgg ttgggccttc gtgttggcgc tctgcggctg
     1561 gctagctgtc atcgcctggc atgtcggccg cagcatgcgc ccaacgcgcc ccgatccgct
     1621 cgcgcgtgct tatgcgcgcc tgtgccgcaa gctcgcgcac gtcgtgccgc gtgctccaca
     1681 ccagggaccg ctggattttg ccgccgcgct cagcgcgcgc cgtccgcagg tgcaggccgc
     1741 ggtactgccg ctgctcacac gctacgccga gcttcgttat ggtcccccaa caccgcaaac
     1801 gcgcgcgcag gacatccgcg cgttccgtga ggcggtgaga cggctgtcac tgccgcgcgg
     1861 cgagatacgt taactcgcag cgggaaatgt taagtcgccg caccacctgc ggctgaaatg
     1921 ttagatgcgt cgcccgttcc gggcgcgctg ccgttcgtcg gcgacgagag tgagtccaac
     1981 ccgtcgactg ctccacagaa ccactgggca aatgcagtga tattgctgca ccgcggctcc
     2041 aaacagggaa acgctatgga ccgtttaggt cccgttcaaa ctggcatcga cgcgcgtcgc
     2101 cgcagcggcg gcttcacgct catcgagctc atgatcacgg tcgccatcgt ggcgatcctg
     2161 gccgccatcg cgctgccttc ctactccatt ttcatgaaga ggtcgcggcg cggcgatacc
     2221 gagtcgagca tgatggacat cgcgcagcgc gagcagcagt acctgctcga cacccgcacc
     2281 tatgcgccga atgtcgccgc gctcaacacc acggtgccgg cggacgtgac cgcctactac
     2341 accatccaga tttgccagac ggccgtgccg tgcgccgcac cgggaggtgc gccgccgact
     2401 ttcgtcgtca ttgccacccc catcgccggc accgcccagg ccggcgatcc cacgctgacg
     2461 ctcgacaaca ccggggtcaa aggccccgcc ggcgtgtggt gagtacccgg agaaaactca
     2521 tgcgcgccag ctgtcacaac tgctacggat ctgcggcgtc tgcacgccgg cgccagcgcg
     2581 gctttaccct cgtggaggcg atcgtcacgg tgagtatcgc cgccatcctc gccaccgtag
     2641 cggtacctgc cttcaacggc atcatcgcga accagcgggc caagactctg gcctcggcgc
     2701 tgtacgccac gctcagcaag acgcgctccg aggcgctcac cctcaacggc aacgtcaccc
     2761 tgcagcccaa ggcgggtggc tggggaaacg gctggcagat gctcgatccc aacaacaacg
     2821 tgctcgatga ctacaccacg acatcgggca tcaccatcgc gggcccggcc gcggtcacct
     2881 accgcgcctc cggccgcctg ccgacaggtg cggcaccgat gttccagatc tcgaccatga
     2941 gcggcgccac ggtcaattac caatgcgtgt ccgtcgatct gggcggacgc ccctacatgt
     3001 ctccgagcca gacatgctga gcgcgcgcat cctcccggcc gcacgcggca gctcgatggt
     3061 cgaggtgctg gtgacgctca ccattctcgc cttcggtctg ctcggcgtcg cctgcctgca
     3121 gagcaag
//
LOCUS       DATDMP010000602         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_428515, whole genome shotgun sequence.
ACCESSION   DATDMP010000602 DATDMP010000000
VERSION     DATDMP010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428515"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VI195_10620"
     CDS             <1..689
                     /locus_tag="VI195_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824884.1"
                     /translation="DSPKGYIVEKLLLAAAPAASRQPVSGAALRYVDWLFPGILGMNM
                     MFSCLFGVGYVVLRYRKSGFLKRLYATPLTAFEFLSAQVLSRLGLILFVTVVLYGGIG
                     AIIGFHNAGSTLLLLLLAIVGALSMIALGLTIAARFSSEELVGGLLNLLTWPMMLLSG
                     IWFSLEGSPRWVQWVAHLFPLTHVLDAARAVMLDGAGLAQIAPHLLFLGATALAFLAF
                     GAWSFRWRID"
     gene            727..1740
                     /locus_tag="VI195_10625"
     CDS             727..1740
                     /locus_tag="VI195_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY6824885.1"
                     /translation="MRFLSDNTASACPEILAAMAAANHGRVPAYGADEWTSRLDRCFS
                     DFFGTEVRAFVVTTGTAANALSLATLCPPYGAIFAHEEAHIVTDECGAPGFFTGGAQL
                     VLLPGEHGRLAPERLRAALAAHPVSLHTVQAAVLSLTQATEAGTVYGSEELAQLAASA
                     HERGLKVHMDGTRLANAVAFLDCHPADITWRAGIEVLSFGATKNGALAAEAVVFFDRS
                     LVRDFELYRKRAGHLLSKSRFAAVQLLTYLESGLWRRNAERTNRLAQRIGRAAAATVL
                     HPVEANEVFLRLGVERRQQLRAQGFEFEDWGAPARGDARLVVSWDQPEEDVQALCAAL
                     ARL"
     gene            complement(1745..>2081)
                     /locus_tag="VI195_10630"
     CDS             complement(1745..>2081)
                     /locus_tag="VI195_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6824886.1"
                     /translation="YAAVAARGKEIATLRAIGFRGTPVVTSVMVEALLLALLGGALGA
                     LLAYLLFNNLSVSTLGQSFTQVVFNFKVTPQLVARGLIIAIVIGMIGGFMPALRAARL
                     PVSASLREQ"
BASE COUNT          308 a          715 c          687 g          371 t
ORIGIN      
        1 ccgattcacc caaggggtac atcgtcgaga aactgctgct cgccgccgcg cccgccgcga
       61 gccgccagcc agtgagcggt gccgccctgc gttatgtcga ctggctgttc ccgggcatcc
      121 tcggcatgaa catgatgttc agctgtctgt tcggcgtcgg ctacgtggtg ctgcgctacc
      181 gcaagagcgg ctttctcaag cggctatacg cgaccccgct caccgcgttc gagtttctca
      241 gcgcacaggt gttatcacgg ctcggcttga ttctgttcgt gaccgtggtc ctgtacggtg
      301 gcatcggcgc catcatcggg tttcataatg ccggcagcac gctgttgctg ctgctgctgg
      361 cgatcgtggg cgcgctgtcg atgatcgcgc tcggcctcac catcgccgcg cgcttctcga
      421 gcgaggaact ggtgggaggg ctgctcaacc tgctcacctg gccgatgatg ctgctctcgg
      481 gcatctggtt ctcgctcgag ggctcgccgc gctgggtgca gtgggtggcg cacctttttc
      541 cgctcaccca tgtgctcgac gccgcgcgcg cagttatgct cgatggggcc ggacttgcgc
      601 agatcgcacc gcacctgctg tttctcggtg ccactgcgct cgccttcctc gccttcggcg
      661 cgtggtcgtt ccgctggcgc attgattgac gaatcgcatt gcctgagcca ctgaggagta
      721 ccgctggtgc gttttctgag cgacaacact gcgagcgcct gtccggagat cctggcggcc
      781 atggcggctg ccaaccacgg ccgggtaccc gcctacggcg ccgacgagtg gaccagccga
      841 ctcgatcgct gcttcagcga tttcttcggc accgaggtgc gcgccttcgt ggtgaccacc
      901 ggcacggccg ccaacgcgct gagtctcgcc acgctgtgcc caccgtacgg agcaatcttt
      961 gcgcacgagg aagcgcacat cgtcaccgac gagtgcggcg cccccggctt cttcaccggc
     1021 ggcgcgcagc tggtactgtt gccgggcgag cacggtcgcc tcgcgccgga gcgcttgcgc
     1081 gccgctctcg ccgcccaccc ggtgagcctg cacacggtgc aggccgcggt gttgtcgctg
     1141 acgcaggcga ccgaggccgg cacggtctat ggcagcgagg agctggcaca actcgccgcg
     1201 agtgcccatg agcgcggcct caaggtgcac atggacggga cgcgtctcgc caacgcagtg
     1261 gcatttctcg actgccatcc ggctgatatc acctggcgag ccggcatcga ggtgctctcc
     1321 ttcggggcta ccaagaacgg cgcgctcgcc gccgaggcgg tggtgttctt cgaccgctcg
     1381 ctggtgcggg acttcgagct gtatcgcaag cgtgccggac acctgctgtc gaaatcccgc
     1441 ttcgcggcgg tgcagctgct cacctacctg gaaagcggcc tgtggcgcag aaacgccgaa
     1501 cgcaccaatc gactcgcgca gcgcatcggc cgtgccgccg ccgccacagt gctccacccg
     1561 gtcgaggcca acgaggtgtt cctgcggctc ggcgtcgagc gccgccagca gctgcgcgct
     1621 cagggcttcg agttcgaaga ctggggggcg ccggcgcgcg gtgacgcgcg cctggtcgtg
     1681 tcctgggatc agcccgagga ggacgtacag gcgctgtgcg cggcgctcgc gcggctctga
     1741 aggctcactg ttcgcgcagg ctcgcgctca ccggcagccg cgccgcgcgc agcgcgggca
     1801 tgaagccgcc gatcataccg atgacgatgg cgatgatgag gccgcgtgcc accagctgcg
     1861 gcgttacttt gaaattgaac accacctggg tgaagctctg tccgagtgtc gacaccgaca
     1921 gattgttgaa cagcaggtag gcgagcaacg caccgagcgc tcccccgagg agcgccagca
     1981 gcagcgcctc gaccatcacc gaggtcacca ccggggtgcc gcggaagccg atcgcccgca
     2041 atgtggcgat ctccttgcca cgcgccgcga ccgcggcata c
//
LOCUS       DATDMP010000603         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_650520, whole genome shotgun sequence.
ACCESSION   DATDMP010000603 DATDMP010000000
VERSION     DATDMP010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650520"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1462
                     /locus_tag="VI195_10635"
     CDS             107..1462
                     /locus_tag="VI195_10635"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY6824887.1"
                     /translation="MSQANASYAARDLAHVWHPCTQMKDHETLPPIPIRRGSGAWLED
                     YDGRRYLDAVSSWWVNLFGHANPRINAAVRAQLERLEHVILAGFTHEPVIALSESLTA
                     LAPAGLTRCFYADNGSSAVEVAVKMSFHYWRNTGHAGKRRFITLANSYHGETLGALAV
                     GNVELYKEIYRPLLMDVITAPSPDCYQRDPAHSWAEHSRRRFADMEALLAQHAHETCA
                     VIVEPLVQCAAGMRMYDPVYLTLLRAACDAHQVHLIADEIAVGFGRTGTLFACEQAGI
                     RPDFMCLSKGLTGGYLPLSSVLTTEPVYAAFYDEYTKLTAFLHSHSFTGNPLACAAAL
                     ATLAIFREDRTLERNRALAAHLGARARELAAHPHVAEVRQRGMIVAVELAADKAARTP
                     YPWQERRGLTVYRHALARGVLLRPIGNVIYFMPPYVVTTEEVDLMVEVAREGIERATC
                     G"
     gene            1453..>2074
                     /locus_tag="VI195_10640"
     CDS             1453..>2074
                     /locus_tag="VI195_10640"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY6824888.1"
                     /translation="MRLTRAYVDAALEPGARVVLSGSAAGHLTRVLRLRPGACVTLFN
                     GRGGEYAAGIERVLRSEVTLVIGEHRTIERESPFPLTLAQGVSRGERMDLVVQKATEL
                     GVTRLVPVITERSIVRLDEEQSDRKSSHWRAIAIAACEQCGRNRLPEVALPAQLPAFL
                     RLPAGESVRLLLSPVAARRIEDVPRPANGATVLIGPEGGLSEEEQAL"
BASE COUNT          320 a          724 c          703 g          327 t
ORIGIN      
        1 ggtgaggagc gacaatccct ctgtgcgttg ctccagaaag tgctgaacgg gcgctggcga
       61 ctgcggttcc atccactgcc cgagagccca tggtaaaagg accctcatga gccaggcgaa
      121 tgcatcctat gcggcccggg atcttgcgca cgtgtggcac ccatgcacgc agatgaaaga
      181 ccacgagacc ctgccgccga ttcccatccg gcgcggcagc ggagcctggc tcgaggacta
      241 cgacgggcgg cgctacctcg atgccgtcag ctcctggtgg gtgaacctct tcggtcacgc
      301 caacccgcgc atcaacgcgg ccgtgcgcgc gcagctcgag cgcctcgagc acgtgatcct
      361 cgccggcttc actcacgagc cggtcattgc gttgtcggag tccctgacgg cgctcgcgcc
      421 agccggcctg acgcgttgct tctacgccga caacggctca tcggccgtcg aggtggcggt
      481 gaagatgagc ttccattact ggcgcaacac cggtcacgcg ggcaagcgcc gcttcatcac
      541 gctcgccaac agctatcacg gcgagaccct cggcgcgctc gcggtcggca acgtcgagct
      601 ctacaaggag atctaccgac cgctgctcat ggatgtgatc accgcgccct cccccgactg
      661 ctaccagcgc gacccggcgc acagctgggc agagcactcg cgccggcgtt tcgccgacat
      721 ggaagccttg ctggcgcagc acgcccacga aacctgcgcg gtgatcgtgg agcccctggt
      781 ccagtgtgcc gcgggcatgc gcatgtacga tccggtgtat ctcacgctgc tgcgcgctgc
      841 ctgcgacgcg caccaggtgc atctgatcgc cgacgagatc gccgtgggtt tcgggcgcac
      901 cggcacgctg ttcgcctgcg agcaggccgg catccgtccc gacttcatgt gtctgtccaa
      961 gggcttgacc gggggctacc tgccgctgtc gagcgtgctc accaccgaac cggtgtacgc
     1021 cgcgttctac gacgaataca ccaagctcac ggccttcctc cactcgcaca gcttcaccgg
     1081 taacccgctc gcctgcgccg cggcgctggc aacgctggcg atcttccgcg aagaccgcac
     1141 gctcgagcgc aatcgcgcgc tggccgcgca tctgggtgcg cgtgcgcgtg agctggcagc
     1201 gcatccgcac gtggcggaag tgcgtcagcg cggcatgatc gtcgcggtgg agctcgccgc
     1261 cgacaaggcg gcgcgcaccc cctacccctg gcaggagcgg cgcgggctca ccgtctaccg
     1321 gcacgcgctc gcgcgtggag tgctgctacg gccgatcggc aacgtcatct acttcatgcc
     1381 gccgtacgtc gtcacgaccg aggaggtcga cctcatggtg gaggtggctc gcgaagggat
     1441 cgagcgcgcc acatgcggct gacgcgtgcg tatgtcgatg cggcgctcga gccgggcgca
     1501 cgcgtcgtgc tcagcggcag tgccgccggc catctgacgc gcgtgctgcg cttgcggccc
     1561 ggcgcctgcg tgacgctctt caatggacgc ggcggcgaat acgccgccgg catcgagcgc
     1621 gtgctgagga gcgaagtcac gctcgtgatc ggcgagcacc ggacgatcga gcgcgaatcg
     1681 ccttttccgc tcaccctggc gcagggagtg tcgcgcgggg agcgcatgga cctggtggtg
     1741 cagaaagcca cggagctcgg cgtgacgcgt ctcgtcccgg tgatcaccga gcgcagcatc
     1801 gtgcggctcg atgaggagca gtccgatcgc aagtcgagcc actggcgcgc catcgcgatc
     1861 gcggcctgcg agcagtgcgg ccgcaaccgc ctgcccgagg tcgcactgcc ggcgcaactg
     1921 cctgcgtttc tgcgtctgcc ggcgggcgag agcgtgcggc tgctgctctc gccagtggcc
     1981 gcgcggcgca tcgaggatgt gccgcgcccc gcgaacggcg cgacggtgct gatcggtccc
     2041 gagggcggcc tgtcggagga ggagcaggcg ctcg
//
LOCUS       DATDMP010000604         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_428987, whole genome shotgun sequence.
ACCESSION   DATDMP010000604 DATDMP010000000
VERSION     DATDMP010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428987"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..847
                     /locus_tag="VI195_10645"
     CDS             290..847
                     /locus_tag="VI195_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY6824889.1"
                     /translation="MAQGRLSGVKVAILATDGFEQSELLEPRKALQEAGASTEVVAPK
                     RGTIRGWNHTEWGQEVPVDKDLQNADPAHYDALVLPGGVMNPDKLRAIPAAVNFAKSF
                     FAADKPVAAICHGPWTVIESGAAKGHRMTSWPSLRTDLRNAGAEWVDEEVVVDGKLVT
                     SRKPDDIPAFNRATIELIAQNRDSS"
     gene            866..1375
                     /locus_tag="VI195_10650"
     CDS             866..1375
                     /locus_tag="VI195_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY6824890.1"
                     /translation="MKFVILATLTAAAPLAFAAGAASDASFYRTLATGGMAEVDLGQL
                     AEQKSTDPKVKDFAQMMVKDHSAANAKLEALAASKHVALPKTLDTSHAATKTRLEGMS
                     GSNFDKSYVESQLKAHEKTVDLLEKEISSGQDADAKAFAQSVLPTVQHHLQAVRNLAS
                     EQGVKSASR"
     gene            complement(1372..1830)
                     /locus_tag="VI195_10655"
     CDS             complement(1372..1830)
                     /locus_tag="VI195_10655"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEY6824891.1"
                     /translation="MWRRVAAWFRWRTERAFAARSIVASNKSTRGRSASASKRRAPRR
                     SRSPSHGRRSAPARGLSHPRRWSAEVMKRSDALDLEAGVFTQRSPRAIAASLKRSALA
                     SRRRKSEPFRSAMSMLNFHINRSGRGLSAQRREILERAKGELRRLFGREG"
     gene            1961..3118
                     /locus_tag="VI195_10660"
     CDS             1961..3118
                     /locus_tag="VI195_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6824892.1"
                     /translation="MSAPDESERSPSLIRELAHELRDALSPLASSVDLARLRKFDAEA
                     SRLLAEKVERGLRRTLAILDSFVLAEQAEHATLPLQVQPVALEAILQGARTMVGEGAH
                     FSFLSAGETVTVRADQGQSARVLAAVLQHAAALASADRPVEVHTEADPRRPRVRVHIR
                     IDPDNVPRGDWFASYRGGDGRMALRTAQQIMRLQGGTLALANDSAGAFELILQFADAT
                     QPQEERHCPPSLASRPADGSTVAASRTRSAHTAGRSTAHSAAGERVLIVDDSAEVRRA
                     YREALVALGYTVLEAADAEQALSALEDASADVALIDIHLPRMNGYRLAQAIRARAGGG
                     IYLVMLSGMALDAVTRGLAREAGFDDSLDKMAGPIALRELLEAGRTRGGSQ"
     gene            3822..>4100
                     /locus_tag="VI195_10665"
     CDS             3822..>4100
                     /locus_tag="VI195_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824893.1"
                     /translation="MAALRLGQHLLMPVVLAVLLALVLSGIVEWLRRYRVPRGFSALV
                     LVLLIGLAVGSVLHAVEAPARQWIESAPRILRIIERKTRAAQSIVRRLD"
BASE COUNT          738 a         1318 c         1364 g          680 t
ORIGIN      
        1 aggctggccg cggcggccgc agtgttgtgg atcctgattc tgaccagtct cggtcttgcg
       61 gacgtgctga cgcgagtgta ggcgtcgccg gtcgcagcag aagagaagct cgggctgaag
      121 ggctgcggcg cgcatcataa ctgttggttt ggatccagca tcgccgacaa gagcttgcga
      181 cccgcaccga cagactcgcg gggccttggt ggccaaagtt tgtatggggt ggggctatgc
      241 gctacgcatg cctcagccag cgcactcggt gagacgacgg agacgggcga tggcacaggg
      301 cagactgagc ggagtcaagg tcgcgatcct cgcgaccgat ggattcgagc aatcggagct
      361 tctcgagccg cgcaaagcgc tgcaggaggc cggtgccagc accgaggtcg tcgctcccaa
      421 gcgaggcacc atccgcggct ggaaccatac cgagtgggga caggaggtcc cggtcgataa
      481 ggacctgcag aacgccgatc cagcgcacta tgacgcgctc gtgctaccgg gcggcgtgat
      541 gaatccggac aagctgcggg cgattcctgc cgcagtgaat ttcgcgaagt cattcttcgc
      601 ggccgacaag ccggtggcgg cgatttgcca cggaccctgg acggtgatcg aaagcggcgc
      661 cgccaaggga catcgcatga cttcgtggcc ctcgctgcgc accgatctca ggaatgccgg
      721 cgcggagtgg gtggatgagg aggtggtcgt cgatggcaag ctggtgacca gccggaagcc
      781 ggacgacatc cccgccttca accgcgccac catcgagctc atcgcccaga accgtgacag
      841 cagttgagca ctgaaggagc ctcctatgaa attcgtgatt ctggccacct tgacggcggc
      901 agccccgctc gcctttgcag cgggcgccgc atccgatgcc tcgttctacc ggaccctcgc
      961 cacaggcggc atggccgagg tcgatctcgg ccagctcgcc gagcaaaaaa gcaccgatcc
     1021 caaagttaag gacttcgcgc agatgatggt gaaggaccac tccgcagcca atgcgaagct
     1081 cgaagcgctg gcagcatcta agcacgtcgc tctgccgaag acgctcgaca cctcgcacgc
     1141 ggcgaccaag acccgtctcg aaggcatgag cggaagcaac ttcgacaagt cgtacgtcga
     1201 gtcccagctg aaggctcacg agaagacggt cgatctgctc gaaaaggaga tctcctcggg
     1261 gcaggacgcc gatgccaaag ccttcgccca gtcggtactg ccgaccgtac agcaccatct
     1321 gcaggcggtg cgcaacctgg cgagcgaaca aggcgtgaag agcgcgagcc gctaaccctc
     1381 gcgaccgaac agcctgcgca gctctccctt ggcgcgctcg aggatctcgc gccgctgcgc
     1441 cgagagaccg cggcccgaac ggttgatgtg gaagttgagc atcgacatcg ccgagcggaa
     1501 cggctcggac ttacgacgtc gactggcgag cgcggatctc ttcagcgagg ccgcaatggc
     1561 gcggggcgag cgctgagtga ataccccggc ctcgagatcg agcgcatcgc ttcgcttcat
     1621 cacctcggcc gaccagcgcc gtggatggga gaggccgcgc gcaggcgcgc tccgccggcc
     1681 atgcgagggg ctccgggacc ggcgcggtgc gcgccgcttg cttgcgctgg cgctcctgcc
     1741 gcgagtgctc ttgttggaag ccacgatcga ccttgccgcg aatgctctct cagtccgcca
     1801 ccgaaaccac gctgccacgc gcctccacac gggtcgatcg gtggcctgag cagaacatcg
     1861 taggactgtt gtaggctagc tcctacagct tggcgctggt cgcttgcatt gaaataggct
     1921 ggcgccgccg gaagctcgct aggtgttcca ccggtaaagc atgtcagcac ccgacgaaag
     1981 cgaacgcagc ccctcactga tacgtgagct cgcgcacgag ctgcgggatg ctttgagtcc
     2041 tcttgcctcc tcggtggatc tcgcgcgact gcgcaagttc gatgccgagg ccagccggct
     2101 gctcgccgag aaagtcgagc gcggtttgcg ccgcacgctg gcgatactcg atagcttcgt
     2161 gctcgccgag caagcggaac acgcgacctt gccgctgcag gtgcagccag ttgcactcga
     2221 ggcgatcctg cagggcgcgc gcaccatggt cggcgaaggc gcgcacttca gtttcctgag
     2281 cgctggtgag accgtcacag tccgagccga tcagggacag tccgcgcgcg tactcgcagc
     2341 cgtcttgcag catgcggcgg cgctcgcctc cgcggaccgg ccggtcgagg tgcacacgga
     2401 agcggacccg cgacggccgc gggtccgtgt ccacatccgc atcgatccgg acaatgtgcc
     2461 gcgcggggac tggtttgcaa gttatcgggg cggcgatggc cgcatggcgt tgcgcaccgc
     2521 acagcagatc atgaggttgc agggaggcac actcgcgctc gcaaatgaca gcgcaggcgc
     2581 gttcgagctg atcctgcagt tcgcggacgc aactcaacct caggaggaac gccattgccc
     2641 accctcactt gcctcgcggc ccgcggatgg ctccacggtt gcggccagtc gtacgcgtag
     2701 cgctcacacg gccggccgca gcacggctca ttccgccgcc ggtgaacgcg tcctgatcgt
     2761 cgacgacagc gcggaagttc gccgtgcata tcgcgaggcg ctggtagcgc tcggctatac
     2821 ggtgctcgaa gccgccgatg cagagcaggc gctgagcgca ttggaggacg cgagtgcgga
     2881 cgtggcgctc atcgacattc acctgccgcg catgaacggc taccgtctcg cacaggcgat
     2941 ccgcgcacgc gctggaggtg ggatctatct ggtgatgctg agcggcatgg cgctcgatgc
     3001 ggtaacacgc gggctggcgc gggaagcggg tttcgatgac tcgctggaca aaatggcagg
     3061 tccaatcgcg ctgcgcgagc tcctcgaggc ggggcgaacg cgtggcggaa gtcagtaaag
     3121 aaacagtctc ggaaacgacg aacgagcatt cggcggagtc atcgcgaaac ccgagccctt
     3181 gcgctcttcc ctgaggtgcc accgctccga gttctgggcc atccttggcc cgccgcctcg
     3241 tcctttgagg ttcaccgccc gtccagggaa accggaatcc tgccgaagac aacgctcgcc
     3301 gccccgagac tgtttaggtg cgtcggaccg agtaggggca gcttcccggc gacgcctgac
     3361 tttgttcacg ccacaatggg cgagaaggaa cttacccacg agaccgagct gccgctgacg
     3421 gtgcgcgacc tcaatgcgcg tcggatgagg accgagatgc acgtcggtga gctcctacac
     3481 ttttcaccgc cgtatgccgc cgacagcaga acaggtgctc tgccgacggt gtagatgcga
     3541 gtcccttccg atgattgctg cggctgcgga gcctggatcc gtttcggctg ctgcgtagcg
     3601 ccatcaacat gaagaatgtg gagcctgcca atgcaccgac gagagcattg ctgcgaccgt
     3661 cattgaacgc cgttccgcag ggcggtgcgg gcgcgacgcc tcacggctcc gccccggaag
     3721 atttcgcacc ccggtcgcgg tcgcctgccc atgccgcggg ctggcgtcgc cccgggcggg
     3781 ctatcgcccg gccgctctgg ctcatcgcca tatgcgtcgc ggtggctgcg ttgcgcctgg
     3841 gacaacatct actgatgccg gtcgtgctcg ccgtgctgct ggcgttggtg ctctcgggta
     3901 tcgtcgagtg gctgcggcgt taccgcgttc cacgcggctt cagcgcactc gtgctggtgc
     3961 tactcatcgg gctggcagtt ggcagcgtgc tgcatgccgt ggaagcgccg gcacggcagt
     4021 ggatcgagag cgcaccacgg atcctgcgca tcatcgagcg caagacgcga gccgctcagt
     4081 cgatcgtgcg ccggcttgat
//
LOCUS       DATDMP010000605         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1872499, whole genome shotgun sequence.
ACCESSION   DATDMP010000605 DATDMP010000000
VERSION     DATDMP010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872499"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /gene="pstB"
                     /locus_tag="VI195_10670"
     CDS             complement(<1..957)
                     /gene="pstB"
                     /locus_tag="VI195_10670"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005171697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY6824894.1"
                     /translation="MSEETLPARRARPDRDGGRMSAAGGGDGRAGDDAAPQPDAEEGG
                     ATGVGSASGARSAAAAAAAASAAGGARHLGEGGLNLKPSGRHPAATDAGVQADGATVE
                     NPVFSCREVSVFYGAKQALKDVSVDVGRRQVLAMIGPSGCGKSTFLRCLNRMNDTIPG
                     ARVSGRILLDGRDIHDESIDVAQLRARIGMVFQKPNPFPKSIYDNVAYGPRIHGLARS
                     RAHLDDIVCTSLTRAGLWDEVKDRLGSSGTGLSGGQQQRLCIARTIAVQPEVILMDEP
                     CSALDPIATARIEDLIDELRESYAIVIVTHSMQQAARVSQRTA"
     gene            complement(964..1770)
                     /gene="pstB"
                     /locus_tag="VI195_10675"
     CDS             complement(964..1770)
                     /gene="pstB"
                     /locus_tag="VI195_10675"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY6824895.1"
                     /translation="MGTPQPRLGTAPAPAAGGAVKFEVRHLDFYYGNVHALKDINLTL
                     SDRQITAFIGPSGCGKSTLLRVFNRMYALYPGHRASGDVRLDGEDILAPSVDVAALRF
                     RVGMVFQKATPFPMSIFDNVAFGLRLSRRISRSELGDRVEEALRDAAMWEEVKDKLHE
                     DGRGLSGGQQQRLCIARTLALRPEVILFDEPTAALDPISTLRVEETLQSLRESYCIAI
                     VTHNLQQAARVSNVTAFMYLGELVEIGPTEQMFTAPRNRRTDDYVTGRFG"
     gene            complement(1770..2618)
                     /gene="pstA"
                     /locus_tag="VI195_10680"
     CDS             complement(1770..2618)
                     /gene="pstA"
                     /locus_tag="VI195_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY6824896.1"
                     /translation="MSRRAIRKLINAVALGLSALATGIGLFFLGAILWTLVRSGLAGM
                     SLQLFTAMTPPPGSSGGLLNAIYGSVVMTVIGILIGGPIGVLAGTYLAEYGRTARIST
                     LIRFVNDVLLSAPSIIVGLFVYELLVVRVGHFSAIAGAVALAIIAIPVTVRTTEDMLN
                     LVPQGMKDASTAMGAYHWRTITSVIYPAARSGIMTGLLLAIARISGETAPLLFTALNN
                     QFWSSNLAAPIANLPVVIFQFALSPYSNWQQLAWSGALIITASILILNIAARFVVSSL
                     GPRGRN"
BASE COUNT          488 a          903 c          932 g          434 t
ORIGIN      
        1 ggccgtgcgc tgcgacaccc gtgccgcctg ctgcatggaa tgcgtcacga tcacgatcgc
       61 gtaggattcg cgcaattcat cgatcaggtc ctcgatgcgc gccgtggcaa tcggatcgag
      121 tgccgagcac ggctcgtcca tgaggatcac ctccggctgc accgcgatcg tacgtgcgat
      181 gcacagccgc tgctgctggc ctccggagag cccggtgccc gagctcccca gccggtcctt
      241 gacctcgtcc cacagacccg cgcgcgtcag gctggtgcag acgatgtcat cgagatgtgc
      301 gcggctgcgc gccaacccat ggatgcgggg accgtaggcg acgttgtcat agatcgactt
      361 ggggaagggg ttgggcttct ggaacaccat gccgatgcgc gcgcgcagct gggcaacatc
      421 gatgctctca tcgtgtatgt cacgtccatc gagcaggatg cggccgctga cgcgcgcccc
      481 ggggatggtg tcgttcatgc gattcaggca gcgcaggaag gtcgacttgc cgcagcccga
      541 gggaccgatc atggccaaca cctggcgccg gccgacatcg accgacacgt ccttcagtgc
      601 ctgcttggcc ccatagaaaa cgctcacctc acggcacgag aacaccgggt tctcgaccgt
      661 cgccccatcc gcctgcactc ccgcatcggt cgcggccgga tgcctgccgc tcggcttgag
      721 attcagtcca ccctcgccga gatggcgcgc gccgccggct gcggaggcgg cggcagccgc
      781 ggcagctgcg gagcgcgcgc ctgacgcgct gccaacgccc gtggcgccgc cctcctcggc
      841 atcaggctgc ggagcggcgt catcgccggc gcgaccatcg ccgccgcccg ccgcactcat
      901 gcggccgccg tcacgatcgg gccgtgcgcg tcgtgcgggc aaggtctctt cgctcacggg
      961 ccatcagccg aaacgcccgg tgacatagtc atcggtgcgg cggttgcgcg gtgcagtgaa
     1021 catctgctcg gtcggcccga tctccacgag ctcaccgagg tacatgaagg cggtcacgtt
     1081 ggagacgcgg gccgcctgct gcaggttgtg ggtcacgatg gcaatacaat agctctcgcg
     1141 caggctctgc agggtttcct cgacccgcag ggtggagatc gggtcgagcg cggcggtcgg
     1201 ctcatcgaac aggatgacct ccggccgcag cgcgagcgta cgggcaatgc acagccgctg
     1261 ctgttggccg ccggagaggc cgcggccgtc ctcgtgcagc ttgtccttga cctcctccca
     1321 catcgcggcg tcgcgcagcg cctcctccac gcggtcgccg agctcgctgc gtgagatgcg
     1381 cctcgacagg cgcagcccga aagccacgtt atcgaagatc gacatcggaa acggtgtcgc
     1441 cttctggaac accatgccga cgcgaaagcg caaggccgcc acatccacgc ttggtgcaag
     1501 aatgtcctca ccatcgagcc gcacatcgcc gcttgcgcga tgccccggat aaagcgcgta
     1561 catgcgattg aacacgcgca gcagcgtcga cttgccgcag ccggagggac cgatgaaggc
     1621 ggtgatctgc cggtcgctga gggtgaggtt gatgtctttg agggcgtgca cgttgccgta
     1681 atagaaatcg agatgccgca cttcgaactt gacggcgccg cccgccgccg gggccggcgc
     1741 cgtgccgagt cgcggctgcg gcgtgcccat cagttgcgcc ccctggggcc gagcgaggac
     1801 accacgaaac gtgcggcgat attgaggatg aggatgctcg cggtgatgat gagcgcaccg
     1861 gaccacgcca gctgttgcca gttcgagtaa gggctcagcg cgaactggaa gatgaccacg
     1921 ggcaggttgg cgatcggcgc ggcgagattg ctgctccaga attggttgtt gagcgcggtg
     1981 aacagcagcg gcgcggtctc gccgctgatg cgggctatcg cgagcagcag cccggtcatg
     2041 atgccgctac gcgcggccgg gtaaatgacg ctcgtaatgg tgcgccagtg ataggcgccc
     2101 atggcggtcg aggcgtcttt catcccctgc ggcaccagat tcagcatgtc ctcggtggtg
     2161 cgtaccgtca ccggtatggc gatgatcgcg agcgcgaccg cgccggcgat tgcggaaaaa
     2221 tgacccacgc gcaccaccag cagctcgtag acgaacagcc cgacgatgat cgagggcgcc
     2281 gagagcagca cgtcgttgac gaagcggatc agggtgctga tgcgcgcggt gcggccgtat
     2341 tccgcgaggt aggtaccggc gagcacgccg atcggccccc cgatcaggat cccgatcacc
     2401 gtcatcacca cgctgccgta gatcgcattc agcaggccac cgctgctgcc aggcggtgga
     2461 gtcatcgcgg tgaacagctg cagcgacatg ccggcgagac cgctgcggac gagcgtccac
     2521 agtatggcgc cgagaaagaa cagcccgatg ccggtcgcga gcgcggaaag cccgagcgcc
     2581 actgcattga tgagcttgcg gatcgcgcgc cggctcatag attggagagc gccgtggtca
     2641 tacgttggag agcgccttgc gcccgagacg cagcagcatc agtcgggccg ccgcgatcac
     2701 cgtgaaggta atgaggaaca acagggcccc gagtgcaacc agcgacgagg tgtagag
//
LOCUS       DATDMP010000606         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_206992, whole genome shotgun sequence.
ACCESSION   DATDMP010000606 DATDMP010000000
VERSION     DATDMP010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206992"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VI195_10685"
     CDS             complement(<1..635)
                     /locus_tag="VI195_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824897.1"
                     /translation="MRLPAAHPPPSLALPAGAHVGVMNLLDAEVTHFHASRQIENSFL
                     KTYTVSWPVSAMLLAALKDRLSQLGLVPVPVAASDAVRRARESCFLDASLAKGLPKEC
                     GPLYAQLAASQGVTALIVLGPGRNDSAHAGGTRHKELPEYLRGWCFVTGGADPTPTLL
                     DMTELLLIGVNGSQAQLVGRGWGGDGKSWTGYHPPPDLKAFPQQQLDQLQPL"
     gene            877..1659
                     /locus_tag="VI195_10690"
     CDS             877..1659
                     /locus_tag="VI195_10690"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY6824898.1"
                     /translation="MAVIGTERVLAVEHWTDTQFTFRTTRDRGLRFENGQFVMVGLML
                     ERPLLRAYSIASANHEEHLDFLSIKVPDGPLTSRLQHVRPGDELLVSRKPTGTLLLAD
                     LRPGVRLYLLCTGTGAAPFLSIIQDPELYARFDEVVLIHGVRWARETEVVRRRIAALE
                     QHELLGEMVRNALRYYPTVTREPHVNRGRLTALLDCGRLLERLHLPPLDAATDRLMVC
                     GSPGFLNESCALLDARGFTISPHTGAPGDYVIERAFVTRTLA"
     gene            complement(1711..2958)
                     /locus_tag="VI195_10695"
     CDS             complement(1711..2958)
                     /locus_tag="VI195_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824899.1"
                     /translation="MSAHLHTHHPMRGRSQPRARASALDQRHVVTLALVALLAAALLL
                     IARTATGAQVAITVNVAPPALPVYEQPPLPSPGYLWVPGYWSWGPEGYFWVPGTWVLP
                     PDPDLVWTPGYWAFSDDAYVWNPGYWGPEVGFYGGVDYGFGYFGHGYEGGYWRDHAFW
                     YNRSVNNVGSTNVTNVYQKTVNNVTVNNISYNGGPGGISARPTAAEQSAAREPRHAAT
                     REQTNHVGAARQNHELLASVNQGHPPIAATPQPNAYSDPNIKPARNAAQGGGSVRGAP
                     PNAPHAPGASGASASTEPARPQTTEPPRRLAEAPPQSAPPPTHERPGPAERATPPPQS
                     RAAPEIGPSERAQAERPETRPGPPPAERAPPPAERAPPHAQGAPAQPEHAPRPSQPQH
                     PSEPREQRSREHPNGRPEDQPPH"
     gene            complement(3101..>3619)
                     /locus_tag="VI195_10700"
     CDS             complement(3101..>3619)
                     /locus_tag="VI195_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824900.1"
                     /translation="PQQLQARLRMALAAGEGTAGAHCIHELWMRGEFAANIEAALAAL
                     WQSAAASIPEWLPMRYFDLLPTLYEVALKFSAARRGRCNLYLVLLDFADRRRGPYGIY
                     VGMSHYSPAQRFDQHKAGIRAAGSVLQRGLEVLTGPVLHLQRIARAEALRLEAQLADA
                     LRDAGLLVEGGH"
BASE COUNT          553 a         1194 c         1266 g          606 t
ORIGIN      
        1 agcggctgca gctgatcgag ctgttgttgc ggaaaggcct tgaggtcggg cggcggatgg
       61 tatccggtcc agctcttccc atcgccaccc caaccgcggc cgacgagctg tgcctgcgag
      121 ccgttgacgc cgatgagcag cagctcggtc atatcgagca gcgttggtgt gggatcggcg
      181 cccccggtca cgaagcacca gccgcgcagg tactccggta gctccttgtg gcgggtgccg
      241 cccgcgtggg cggaatcgtt gcgtcccggg ccgagcacga tgagggcagt gacaccctga
      301 ctcgccgcga gctgggcgta caacggacca cattccttcg gcagaccctt ggcgagcgag
      361 gcatcgagaa agcagctttc ccgagcccgg cgcaccgcat cgctggctgc gacgggcacg
      421 ggcacgagcc cgagctggct caggcgatcc ttgagcgcag cgagcagcat cgcgctcacg
      481 ggccagctga ccgtgtaggt cttgaggaaa ctgttctcga tctggcgcga ggcatgaaaa
      541 tgggtcacct cggcatcgag cagattcatg accccgacgt gcgcgcccgc gggcagcgcc
      601 aggctcggcg gcggatgcgc agcgggtagc ctcacgggaa tgacaccgag cagcgcaaca
      661 cacagcgcgg cccggtatgg cgttggtgtc tctcggctca taccgttccc caagctcggc
      721 gcgagtctag cgctcgcggc agcgttgagg gaaacgcttt ggctacggtg ggcgtcgtca
      781 taacggacgc ttatgagtgc atgacgggaa actttcgagc atgcccgcag cgctcgctac
      841 catgagcgca cacttctttt tccggagcgg cgccgcttgg cagtcatcgg cacggaacgt
      901 gtgctcgcgg tcgagcactg gaccgacact cagtttactt tccgcacgac gcgcgatcgc
      961 ggattgcgct tcgagaacgg ccagttcgtc atggtcggtc tgatgctcga gcgccccttg
     1021 ctgcgcgcct acagcatcgc gagtgccaac cacgaggagc acctggactt cctcagtatc
     1081 aaggtgccgg acggaccgct cacctcgcgg ctgcaacacg tgcggcccgg cgatgagctg
     1141 ctggtgagcc gcaagccgac cggcacgctg ctgcttgcgg atttacgccc cggtgtgcgg
     1201 ctctacttgc tctgtaccgg cacgggggcg gcccccttcc tcagcatcat ccaggatccg
     1261 gagctttatg ctcgcttcga cgaggtggta ttgatccacg gggtgcgctg ggcgcgggag
     1321 acggaggtcg tgcgccggcg catcgcggct ctcgagcagc acgagctgct cggggaaatg
     1381 gtgcgcaacg cgctgcgcta ctacccgacg gtgacgcgcg agccgcatgt caaccgcggg
     1441 cgcctcaccg cgctgctcga ctgcgggcgc ctgctcgagc gtctgcacct tccgccactc
     1501 gatgccgcaa ccgatcggct gatggtgtgc gggagtccgg gcttcctcaa cgagagttgc
     1561 gcgttgctcg atgctcgcgg ctttaccatc tccccgcaca ccggagcacc cggagactac
     1621 gtaatcgaac gcgccttcgt gacgcgcacg cttgcctgag agaaaagtgc cggcgcctgc
     1681 gacaggcgcc ggcatggcga gttgccgccc tcagtgcggc ggttggtcct ccggccgccc
     1741 gttcgggtgc tcgcgtgagc gttgctctcg aggctcactc gggtgctgcg gctgcgacgg
     1801 ccgcggcgca tgctcaggct gcgccggcgc accctgagcg tgcggaggtg cccgttcagc
     1861 cggaggcggc gcacgctcgg caggcggcgg cccggggcga gtctctggac gctcggcctg
     1921 cgcgcgctcg ctcggtccga tctcgggggc tgcccgcgac tgcggcggcg gtgttgcccg
     1981 ctcggcggga ccgggccgct cgtgcgtcgg tggcggcgcg gactgcggag gcgcctcggc
     2041 gagccgccgc ggcggctccg tcgtctgtgg ccgcgcgggc tcagtcgaag cggacgctcc
     2101 cgaggctccc ggtgcgtgcg gggcgttggg cggagccccg cggacggagc cgccgccctg
     2161 cgcggcgtta cgggcaggct tgatgttcgg gtccgaatag gcattcggct gcggtgtcgc
     2221 cgcgatcggc ggatgtccct gattcaccga ggcgaggagc tcgtggttct gcctcgccgc
     2281 gccgacgtgg ttggtctgct cccgcgtagc ggcgtgccgc ggctcgcggg ctgcgctctg
     2341 ctccgccgcg gtcggccggg cgctgatgcc gcccgggccg ccgttgtaac tgatgttgtt
     2401 gacggtaacg ttgttgactg tcttctggta cacgttggta acgttggtgc tgccgacgtt
     2461 gttcaccgag cggttgtacc agaacgcgtg atcgcgccag tagccgccct cgtagccatg
     2521 gccgaaataa ccgaagccgt aatcgacacc accgtaaaag ccgacttcag gcccccagta
     2581 gcctgggttc cacacgtagg cgtcgtcgga aaaagcccag tagcccggcg tccacaccag
     2641 gtcaggatcc ggcggtagca cccaggtgcc cgggacccag aagtaaccct caggtcccca
     2701 gctccagtag cccggcaccc acagatagcc gggcgaagga agcggtggct gctcgtaaac
     2761 cggcagcgcc gggggagcca cgttgaccgt gatggcgacc tgcgcgccag tggcagtgcg
     2821 ggcaatgagc agcaacgcgg cggcgagcag cgccaccagt gcgagtgtca cgacatggcg
     2881 ctggtcgagc gcggaggcac gggcgcgcgg ctgcgacctg ccgcgcatgg gatgatgagt
     2941 gtgcaagtgt gccgacatga tgagtttccg cttgtgactg cgtctggtgc ccaatttgac
     3001 gcactcatcg cggtgccgta atcaggccgc agcagcgctt gcctgtcagt catctccgac
     3061 tatggctgtc cgccgtcgtt gaatcccgac acctgtatgg ctaatggccg ccctcgacga
     3121 ggagtccggc gtcgcgcagc gcgtccgcga gctgtgcttc gagcctcagc gcctcagccc
     3181 gggcgatgcg ctgcaaatgc agcaccgggc ccgtgaggac ctcgagtccg cgctgcagga
     3241 cgctgccggc ggcgcgaatg ccggccttgt gctgatcgaa gcgttgcgca gggctgtagt
     3301 gactcatccc cacataaatg ccgtacggac cgcggcggcg atcggcgaag tcgagcaaca
     3361 ccagatagag attgcagcgg ccgcggcgcg cggcgctgaa tttcagggcg acttcgtaga
     3421 gggtcggcag caagtcgaag tagcgcatcg gcagccactc ggggatggag gccgccgcgc
     3481 tctgccacag ggccgccagt gccgcctcga tgttcgcggc gaactcgccg cgcatccaca
     3541 gctcgtgaat acagtgtgca ccggcggtcc cctcgccggc cgcgagcgcc atgcgcagac
     3601 gtgcctgcag ctgctgcgg
//
LOCUS       DATDMP010000607         4865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1651102, whole genome shotgun sequence.
ACCESSION   DATDMP010000607 DATDMP010000000
VERSION     DATDMP010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651102"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VI195_10705"
     CDS             complement(<1..1864)
                     /locus_tag="VI195_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY6824901.1"
                     /translation="MSDTPARLDAAARAAAIDPCGSVLLEAPAGSGKTSVLTQRFLEL
                     LTTVEDPGEILAITFTRKAAAEMQGRVIRALRGELDAQDAEAAELAALAGAALAHGAA
                     RGWSLDTQPQSLRIQTIDAFNYWLASQLPVASHAGGVLNVTENAGELYQRAARRTLLA
                     AEADATLAPDIRVLFARTDNHWMYLERLIAQMLQERGHWLPFVAREEPEALCRRVNES
                     LANLAAARLASVCALLPERLRQHGERLPGCGRLGCQPADLARWKHLAHLLLTKDDWRR
                     QLVAHRLGPAFADAHVCQQLRDLIDELRGLAGAREALLALKKAPAAQLDEGDAAAIGA
                     LARVLSRAAAELHAEFAQAQRVDYTYVTGAARQALTEGGEPTDLALRAGLALRHILVD
                     EFQDTSLAQVQLLEALTVGWEEGDGRTLFVVGDAMQSIYRFREAEVGLFLRARTAGIG
                     QLKLRTLRLLRNFRAVPELVEFTNEVFAQVFPPLDEPRSGAVRYRASLASRSRAPTLV
                     GPVSLKLFPQGPASEARAVAEYAAQLRQLDPAGAVAILVVAHAHAVPIVDALTARAVP
                     FCAVDLVPLRERLIVRDLVQLTRALFDLSDRAAWLAVLRAPWCGASLRTLTALSS"
     gene            complement(1857..4535)
                     /locus_tag="VI195_10710"
     CDS             complement(1857..4535)
                     /locus_tag="VI195_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY6824902.1"
                     /translation="MFRLSSDISLHFQRGGTLLVPTSQRVRAVQLAHAAAEIAGAQRV
                     WASVDVLTPSRWARRECERLAAEAPAEWPRLLDPTEEWLLWRAATQEAARGFPFLDTG
                     QVAELLQRSSARAAEYGITLHATAPDSEAALLLEAQRGFDARCRALRAASIVSLLPRI
                     GAGGPWGALLARGFAKSPPWMARLTPAAERTPLGSRSASVRALRTSDSRTQMEAIARW
                     CRERLGAQPDARLLVVLPGAPGSRERLAQLIRDVLDPAARFAPLQSARELVAIEGGDS
                     FGSLALPSQALLSLGVLAGETIEMELMCRWLTSPYWMSPEGAARAQLAQGLRERGPAS
                     MPLREFLGALQLAPRELRSAARELDAKLRHAARRVGEGEASPRRWSERFETALAVLGW
                     PGDLRPEGTAHRTRVRWRELLEEFGELARCVGNLELRSALELLRALALRSVYRPGDEG
                     APVTLSPLLADPVVIYDGIWVGSLSADQLPEPPAPDPFLPVRAQVAAGLAEASAAGRR
                     AQAAALLAAWRRSAEELVLSVPARDEDRELLPSPCLAGLTLEEHPAESLWLPLRLHRQ
                     GSIERVIDTRGTAFNPLTPLPYGSRSLTLQSACAFRAYAELRLGASPPESPEPGVPMT
                     QRGLLLHAALQMLWDQLRDSERLAALDERALAALIGDCVGQAARALQAEIGSRRRRAR
                     RVADVQFDLFTELPPALERECERARVLIARLCTLERKRAPFTVEATEHLTELALGGGR
                     VRLRLDRIDRLTTGRAVLDYKSGRAGSPDWFGERPTHPQLLAYLTALGSDVMALATVN
                     LTAREVRFTGVAASADLLPRVRAVIDSPGAATDWSAQQRSWRELIERLIRAFLLGEAQ
                     VDPAPGACDYCHIVDICRIGAHSAAEVSRHTDDSDE"
BASE COUNT          760 a         1708 c         1727 g          670 t
ORIGIN      
        1 cgctcgagag cgcggtcagt gtccgcaagc ttgccccgca ccagggtgca cgcagcaccg
       61 cgagccatgc ggcacgatcg gagagatcga agagcgcgcg cgtcagttgc accagatcgc
      121 gcacgatgag ccgctcgcgc agcggcacga ggtcgacggc gcagaagggc actgcgcgcg
      181 cggtgagggc gtcgacgatg ggtacggcgt gggcatgtgc gaccaccagg atcgccaccg
      241 cgcctgcggg atcgagctgc cgcagctgcg cggcgtactc ggccaccgct cgggcttcgc
      301 tcgcggggcc ctgcggaaag agtttgagcg ataccggtcc gacgagggta ggtgcgcggc
      361 tgcgcgaggc gaggctcgcc cggtacctca cggcgccgct gcgtggctcg tccagcggcg
      421 gaaagacctg ggcgaatacc tcgttggtga attccaccaa ctcgggcacc gcgcggaaat
      481 tgcgcagcag ccgcagagtg cgcagcttca gctggccgat cccggccgta cgcgcgcgca
      541 ggaacagtcc cacctccgct tcgcggaagc ggtaaatcga ctgcatcgca tcgccgacga
      601 cgaacagcgt gcgtccgtcg ccctcctccc acccgacggt gagcgcctcg agcagctgta
      661 cctgcgcgag cgaggtgtcc tggaattcgt ccaccaggat gtgccgcaag gcgaggccgg
      721 cacgcagcgc gagatcggtc ggctcgccgc cctcggtgag tgcctggcgg gccgcgcccg
      781 tgacgtaggt gtaatcgacg cgctgcgcct gagcgaactc ggcgtgcagc tccgctgccg
      841 cgcgcgacag gacgcgcgcc aacgctccga tggctgcggc gtcgccttcg tcgagctgcg
      901 ccgcgggagc cttcttcagc gcaagcagcg cctcgcgcgc accggcaagg ccgcgtagct
      961 catcgatcag gtcgcgcagc tgctgacaca cgtgcgcgtc cgcgaacgcg ggaccgaggc
     1021 ggtgtgcgac cagctgccgg cgccagtcgt ctttcgtgag gagcagatgc gcaagatgct
     1081 tccagcgcgc gaggtcggca ggttggcacc cgagccggcc gcaacccggg aggcgctccc
     1141 cgtgctggcg caatctttcc ggcagtagcg cacagactga cgcgaggcgt gccgcggcca
     1201 ggttggcgag actctcgttg acccgccggc agagcgcttc gggctcctcg cgcgcgacga
     1261 agggtagcca gtgaccgcgc tcctgcagca tttgtgcgat gagccgctcg agatacatcc
     1321 agtgattgtc ggtgcgcgcg aagagcacgc gtatgtcggg cgcgagggtc gcgtcggcct
     1381 ccgcggcgag cagcgtccgg cgcgcggcgc gctgatacag ctcgcccgcg ttctcggtga
     1441 cgttcagtac cccgccggca tgcgaggcga ccggcagctg gctcgccagc cagtagttga
     1501 aggcatcgat cgtctggatg cgcagacttt gcggttgggt gtcgaggctc cagcctcggg
     1561 ccgcgccgtg cgcgagcgcc gcaccggcga gagcggcgag ctccgcggct tccgcatcct
     1621 gcgcatcgag ctcgccgcgc agggcgcgga tgacccgtcc ctgcatctcc gcggccgcct
     1681 tgcgcgtgaa agtgatggcg aggatctcac ccggatcctc gacggtcgtg agcagctcga
     1741 ggaaccgctg ggtgagcacc gaggtcttgc ccgagcctgc cggcgcctcg agcagtaccg
     1801 acccgcaggg atcgatcgcc gcggctctgg cggccgcatc caggcgcgcg ggcgtgtcac
     1861 tcatcgctgt catccgtgtg ccgcgagact tccgcagccg agtgtgcgcc gatgcggcaa
     1921 atgtcgacga tgtgacagta atcgcaggcc ccgggtgcgg gatccacctg cgcctcgcca
     1981 aggaggaagg cgcggatgag gcgctcgatc agttctcgcc agctgcgctg ctgcgcgctc
     2041 cagtcggtcg cggcgccggg ggagtcgatg actgccctga cgcgtggcag gaggtccgcg
     2101 ctcgccgcca cccccgtgaa acgcacctcg cgtgcggtca aatttacggt ggcgagcgcc
     2161 atcacgtccg agccgagcgc ggtgagatag gcgagcagct gtggatgcgt cgggcgctcg
     2221 ccgaaccagt cggggcttcc cgcccgtccg ctcttgtagt cgagcactgc acgcccggtg
     2281 gtgaggcggt cgatgcggtc gagccgcagg cgcacccggc cgccgccgag ggcaagctcg
     2341 gtcaggtgct cggtggcctc gaccgtaaat ggggcccgct tgcgctcgag cgtacacagt
     2401 cgtgcgatca acacgcgcgc gcgctcacat tcgcgctcga gcgccggggg cagctcggtg
     2461 aagaggtcga actgcacatc ggctacgcgc cgtgcgcgcc ggcgacggct gccgatctcg
     2521 gcctgcaacg cgcgtgcggc ttgtccgaca cagtcgccga tcagcgcagc gagcgcgcgc
     2581 tcatcgagcg ccgcgaggcg ctcggagtcg cgcagctgat cccagagcat ttgcagcgcc
     2641 gcgtgcaaca gcaggccgcg ctgggtcatc ggcacgccgg gctcgggtga ctcgggcggt
     2701 gatgccccga ggcgcagctc ggcgtaggcg cggaatgcac aggcactctg caacgtcagt
     2761 gagcggcttc cgtaaggcag tggtgtgagc gggttgaagg cggtgccgcg ggtatcgatg
     2821 acccgctcga tgctgccctg ccgatgcagg cgcagcggta gccagaggct ttcggccggg
     2881 tgctcctcga gagtgaggcc cgcgaggcaa ggactcggca gcagctcacg gtcctcatcc
     2941 cgtgccggca ccgataacac cagctcttcc gcgctccgcc gccacgccgc gagcagcgca
     3001 gccgcctgcg cgcggcgtcc ggcggcactc gcctcggcca gccctgctgc gacttgagcg
     3061 cgcaccggca gaaacggatc gggcgcgggt ggctcgggta gctgatcggc actcagcgag
     3121 ccgacccaga tgccgtcata aattaccacc gggtctgcga gcagcggcga gagggtcacc
     3181 ggtgcgccct catctccggg acgatagacg ctgcgcagcg ccagggcgcg cagcagctcg
     3241 agcgcggagc gcagctcgag attgccgaca cagcgtgcga gctcaccgaa ctcctcgagc
     3301 aactcgcgcc agcgcacgcg cgtccgatgc gccgtgccct cgggccgaag gtctccgggc
     3361 caaccgagca ccgcgagcgc agtctcgaag cgctcggacc agcggcgcgg cgatgcctcg
     3421 ccctcaccca ccctgcgcgc ggcgtgccgc agcttggcat cgagctcgcg cgcggcggat
     3481 ctgagctccc ggggcgcgag ctgcagcgca ccgagaaact cgcgcagcgg catgctcgcc
     3541 ggaccgcgct cgcgcagccc ctgcgcgagc tgcgcgcgcg ctgcgccctc tgggctcatc
     3601 cagtagggcg aagtcagcca gcgacacatc agctccatct cgatggtctc gccggcgagc
     3661 acgccgagcg aaagcagtgc ctgcgaaggc agggccagcg agccgaagga atcgccgcct
     3721 tcgatggcca ccagctcgcg cgcgctctgg aggggagcaa agcgcgctgc gggatcgagc
     3781 acatcgcgga tgagttgtgc gagccgctcg cgcgagcctg gagctcccgg cagcaccacg
     3841 agcagccggg cgtcaggctg cgcgccgaga cgctcacggc accagcgggc gatcgcctcc
     3901 atctgcgtgc ggctatcgct cgtgcgcagt gcgcgcacgc tcgcactgcg ggacccgagc
     3961 ggcgtgcgct cggcggctgg cgtcaacctc gccatccatg gcggactctt ggcgaagccg
     4021 cgcgccagca gagcgcccca ggggccaccc gcgccgatgc gcggcaagag actgacgatg
     4081 ctcgcggcgc gcagtgcacg gcagcgcgca tcgaaaccac gctgcgcctc gagcagcagc
     4141 gccgcctcgc tgtccggcgc ggtggcgtgc agggttatgc cgtactcggc ggcccgcgca
     4201 ctcgagcgct gcagtagctc cgcaacctgg ccggtatcga ggaagggaaa gccacgagcc
     4261 gcttcctggg tggctgcgcg ccacaggagc cactcctcgg tcgggtccag cagccgaggc
     4321 cactcggcgg gcgcctctgc ggcgagccgc tcgcattccc gccgtgccca gcgcgaagga
     4381 gtgagcacat cgacactcgc ccacaccctt tgcgcgccgg cgatctcggc ggccgcgtgg
     4441 gcgagctgca cggcgcgcac ccgctggctg gtaggaacca gcagcgtgcc gccgcgctgg
     4501 aaatggaggg aaatgtcgct agaaagtcta aacaaaaccg ataactataa ccttcatgtc
     4561 gaaatcactt tagcctcgaa gccggtgcga tcacgtcgcg catcacggcc ccgcgagcac
     4621 ggccggaaga ataacccaag cggcgcgcgc gtcctctgcc ctggcgcgca ctgtgactcc
     4681 agccggtctt cacgcgagct cgcgccagcg atgacaggcg atgccccacc cggcctgctc
     4741 ctcccactcc ggtacgcgct cggcgcactg aggcgcggcg agcgcacagc gcgtgcggta
     4801 cacgcagccg ctcgggggtg cgagtgccga tgccggctcg ccgctgcgta cgcgtgagag
     4861 ccgtg
//
LOCUS       DATDMP010000608         2283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1206310, whole genome shotgun sequence.
ACCESSION   DATDMP010000608 DATDMP010000000
VERSION     DATDMP010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206310"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VI195_10715"
     CDS             <1..300
                     /locus_tag="VI195_10715"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY6824903.1"
                     /translation="AAVQEAILRDAEGRAERRERQDVVRRLARLTPRERQVLDLVATG
                     MLNKQIAAELGAAEKTIKVHRGRIMEKMQVRTATALVGLLSRAELRGGGGRLPAP"
     gene            335..904
                     /locus_tag="VI195_10720"
     CDS             335..904
                     /locus_tag="VI195_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY6824904.1"
                     /translation="MITQDTCARLSLLAFAALLLTGCETGPQVRSVTEPGANLSAYRT
                     YSFVAQPGTNRGGNVTPLTTFFETAIAREMSARGYQQVQEGGDLLVNFNARVSEKADI
                     QSTPGPMYGYGYYGYRGGMYMGPEVQTVRYKVGTANIDVVDAKRKVVVWEGIAERELT
                     EEVMRNPQPAIDNTVAKMYAQFPGRAAPP"
     gene            complement(928..2190)
                     /locus_tag="VI195_10725"
     CDS             complement(928..2190)
                     /locus_tag="VI195_10725"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY6824905.1"
                     /translation="MRNQDRASLRLGPGLITGACNDDPGSIGTYSQIGAQFGYTLAWT
                     LLFSFPLLVAVQEISARIARVSGLGIAGNLRRHCPSWLAAALILLLAAANVFNLAADL
                     AAMGAVLRLLIGAPALLYVCLLGTASVLIEVLTGYARYVRLLRWLSLSLLSYVAGAFV
                     VELPWRELAQALLWPPFSMNPQYLLAVLAALGTTISPYLLFWQAQQEVEAGHEGGAAA
                     LLDAIEQAPEEFARIRLDTLTGMGLAAIVALCIVITSASTLHAHGVQSIDNVAQAAEA
                     LRAVGGRFTFTLFALGIIGAGLLALPALSASTAYAVGELLRWRVGRRHARERARAFYA
                     AIGTATALAVGLNFIPLDPMRALYLSSLINGVMSVPLLGAVMCLASNQRVMGALPLPP
                     VLRVLAWLTIVTLACSVAGVTIGWLVRS"
BASE COUNT          426 a          780 c          761 g          316 t
ORIGIN      
        1 gcggcggtgc aggaggcgat cctcagggat gctgagggac gcgccgagcg gcgcgaacgc
       61 caggatgtcg tccgccgcct ggcacgcctc accccgcgcg agcggcaagt gctcgacctg
      121 gtagccaccg ggatgctcaa caagcagatt gccgccgagc tcggcgcggc cgagaaaacc
      181 atcaaggtgc atcgcggccg catcatggag aagatgcagg tccgcacggc cacggcgctc
      241 gtcggcctgc tgtcgcgcgc ggagctgcgc ggcggcgggg ggcgcctgcc agcgccctga
      301 agctggccgt tcatttctat ccgaggaaac atcaatgatc acccaggaca catgcgcccg
      361 cttgagtctg ctggcattcg ccgcgttgct gttgaccggt tgtgagaccg ggccgcaggt
      421 gcgctcggtc accgagcctg gagccaacct ctccgcttac cgcacctact ccttcgtggc
      481 gcaacccggc accaaccgtg gcggcaacgt gacgccgctt accactttct tcgagaccgc
      541 gatcgcgcgc gagatgagtg cgcggggcta tcagcaggtg caggaggggg gcgacctgct
      601 ggtgaatttc aacgcccgtg tcagcgagaa agccgacatt cagtcgacgc ccgggccgat
      661 gtacggctac ggctactatg gctatcgggg cgggatgtac atgggcccgg aggttcagac
      721 ggtgcgctac aaggtcggca ccgccaacat cgacgtcgtc gacgccaaac gcaaggtcgt
      781 ggtatgggaa ggcatcgccg agcgtgagct gaccgaagag gtcatgagga acccgcagcc
      841 tgcgatcgac aataccgtgg ccaagatgta cgcgcagttc ccgggtcgcg ccgcaccgcc
      901 ctgacgcgcg aacgcgcgca ccgctgctca ggaacgcacg agccacccga tcgtcacccc
      961 tgcgacgctg catgccaggg tgacgatcgt cagccaggcg agcactcgca ggaccggcgg
     1021 cagcggcagc gctcccatga cacgctgatt gctcgcgagg cacatcaccg cacccagcag
     1081 cggcacggac atcacgccat tgatcaagga gctgaggtac agcgcgcgca tcggatcgag
     1141 cggtatgaaa ttcagtccga ccgcgagcgc ggtcgccgtg ccgatcgccg cgtagaaggc
     1201 ccgcgcgcgc tcacgcgcgt ggcggcgtcc gacccgccac cgaagcagct ccccgaccgc
     1261 gtatgcggtc gaggccgaga gcgctggcag cgcgagcaga cctgcgccga tgatccccag
     1321 cgcgaacagc gtgaacgtga agcgtccgcc taccgcgcgc aacgcttcgg cggcctgggc
     1381 aacgttgtcg atgctctgca cgccgtgcgc atgcagcgta ctggcgctgg tgatcacgat
     1441 gcacagcgcc acgattgcgg cgagacccat gccggtcaat gtgtcgagcc ggatgcgcgc
     1501 gaactcctcg ggtgcttgct cgatggcatc gaggagcgcc gcggcgccgc cctcgtgccc
     1561 cgcctcgacc tcctgctgtg cctgccagaa cagcaggtac ggactgatgg tggtgccgag
     1621 cgctgcgagc acagcgagca ggtactgcgg gttcatcgag aacggcggcc acagcagcgc
     1681 ctgcgccagc tctctccagg gcagctcgac cacgaacgca cccgccacgt aactcagcaa
     1741 cgacagtgac agccagcgca gcaggcgcac gtagcgcgca tagccggtga gcacctcgat
     1801 gagcaccgat gccgtgccca gcaggcacac gtacagcagc gctggcgccc cgatcagcag
     1861 gcgcagcacc gcgcccatgg ccgccagatc cgccgccaag ttgaacacgt tcgcggccgc
     1921 gagcagcagg atgagcgccg ccgcgagcca tgaggggcag tgccgccgca gattgccggc
     1981 aatccccagg ccgctcaccc gcgcgatgcg agcgctgatc tcctgcactg ctaccagcaa
     2041 cgggaagctg aagagcagcg tccaggcgag cgtgtaaccg aactgtgcgc cgatctgcga
     2101 gtaagtgccg atgctgcctg gatcgtcgtt gcaggcgccg gtgatgagac ccggacccag
     2161 acgcagcgag gcgcggtcct ggtttctcat cctccccgct catacgttcg ttcggcgacc
     2221 ggggagtagg atagcaggac ccaacacgag gccggcggtg ccgacgcgat acccacgccg
     2281 tag
//
LOCUS       DATDMP010000609         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_319040, whole genome shotgun sequence.
ACCESSION   DATDMP010000609 DATDMP010000000
VERSION     DATDMP010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319040"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..>2835
                     /gene="ileS"
                     /locus_tag="VI195_10730"
     CDS             143..>2835
                     /gene="ileS"
                     /locus_tag="VI195_10730"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY6824906.1"
                     /translation="MTDYKPTINLPQTDFPMKADLAQREPAAVRRGEERSTYAKLRQI
                     ARGRPRFVLHDGPPYANGAIHVGHVVNKVLKDIVVKSRSLDGFDSPYIPGWDCHGLPI
                     ELQVEKKHGRPGRKLDARAFRAACRAFAHEQIELQRSDFKRLGILGDWDRPYLTMSPR
                     YEAQQLRAFGRIIANGHLYKGVKPVHWCLDCRSALAEAEVEYEEKVSPAIDVAFRVHD
                     DAELARRLGVSAGEFGPEPVDIVIWTTTPWTLPANQAVALRAEFRYVLAAAHRGRERH
                     RVIVAAELLPACLARFGMSEAAVLAEFEGRALEGLELQHPFLERLVPVVLGEHVTLDA
                     GTGAVHTAPGHGQEDFAIGQRYGLAVLNPVGNDGRFLADTPLVAGLKVDEANPVIIAA
                     LAQAGRLVHEEPFRHSYPHCWRHKSPVIFRATPQWFISMEQAGLREHTLRDIPGVRWT
                     PAWGEQRITGMIESRPDWCISRQRTWGVPIPLFVHKQTGERHPRTLELIEAAAARVEQ
                     GGIDAWFALEPRELLAEEAEHYEKVTDVMDVWADSGLSFECVGAERPEVSAPVELYLE
                     GSDQHRGWFHSSLLMSEALYERPPYRGVLTHGFTVDEKGRKMSKSLGNVIAPQKVMST
                     LGADVLRLWVSATDYANEIAVSDEILRRMADSYRRMRNTLRFMLGNLHGFDPAQHALP
                     AARLVALDRWVLARAHALQAEVLDAYRTYAFHLIYQKVHNFCSVDLGSFYLDVIKDRM
                     YTMPVASPGRRSAQTVMWHIAESMVRWLAPILSFTAEEVWGYLPGERRESVFHETWHA
                     LPEVAADGVDWDALIALRSDVTRELEKLRDAGLIGAPLDARVDVYCTEEEYVRFAALG
                     GELRFLLITSEAQVHRASTSPAGSVPAAHTGSSGVWILAQPT"
BASE COUNT          457 a          934 c          963 g          481 t
ORIGIN      
        1 cgtgagatcg aggtggagtt cgtggcgcga ctgcgcgatg aggagcgctt cgcttcgctc
       61 gaggcgctca ccgagcaaat ggagcgcgac gccgccgaag cgcgccgcat cctgaacgcc
      121 tgacatccga catgattccg acatgaccga ctacaagccc accatcaatc tgccgcagac
      181 cgatttcccg atgaaggcgg atctcgcgca gcgcgagcct gcggcggtgc gacgcgggga
      241 ggagcgcagt acctacgcga agctgcggca gattgcgcgc ggtcggcccc gcttcgtgct
      301 gcacgacggg cctccctacg cgaacggagc gatccacgtc gggcatgttg tcaacaaggt
      361 cctcaaggac atcgtcgtca agtcgcgctc gctcgatgga tttgactccc cctacatccc
      421 cggctgggac tgtcacggat tgccgatcga gctgcaggtc gagaaaaaac acggccgccc
      481 gggaaggaag ctcgatgcgc gcgctttccg cgccgcttgc cgcgctttcg cgcacgagca
      541 gatcgagctg cagcgcagtg acttcaagcg gctcggcatt ctcggcgact gggaccgtcc
      601 gtatctgacg atgtcaccgc gctacgaggc gcagcaactg cgcgccttcg gccgcatcat
      661 cgccaacggt cacctttata agggcgtgaa gcccgtgcac tggtgcctcg attgccgctc
      721 ggcactggcg gaagctgaag tggaatacga ggagaaggtg tcgccggcga tcgatgtggc
      781 gttccgggtg cacgacgacg ccgagctcgc gcggcgcctc ggcgtgagcg cgggggagtt
      841 cggtcccgag cccgtggaca tcgtcatctg gacgacgaca ccgtggacgc tgccggcgaa
      901 ccaggcggtg gcgctgcgcg cggagttccg ctatgtgctc gcggccgctc atcgggggcg
      961 cgagcggcac cgggtgatcg tcgccgccga gctgctccca gcttgcctcg cgcgctttgg
     1021 catgtcggag gcggccgtgc tggccgagtt cgagggacgc gcgctcgagg ggctcgagct
     1081 ccagcatccg ttcctggagc ggctggttcc ggtcgtgctg ggtgagcacg tgacactcga
     1141 tgccggcacc ggtgccgtgc ataccgctcc cgggcacggt caggaagact tcgccatcgg
     1201 ccagcgctac ggactcgcgg tgctgaatcc agtcggtaac gacggccggt tccttgcgga
     1261 caccccgctc gtggcgggcc tcaaggtgga tgaggcgaac ccggtcatca tcgccgccct
     1321 cgcgcaggcc gggcggctgg tgcacgagga acccttccgc cacagctatc cgcactgctg
     1381 gcggcacaag agcccggtga tctttcgcgc gaccccgcag tggttcatca gcatggagca
     1441 ggcgggactg cgtgagcaca cgctgcgcga cattcccggc gtgcgctgga cgccggcgtg
     1501 gggcgagcag cgcatcaccg gcatgatcga gagccgcccg gactggtgca tctcgcgcca
     1561 gcgcacctgg ggcgtgccga ttccgctttt cgtgcacaaa cagaccggcg agcggcaccc
     1621 gcgcacgctc gagctgatcg aggccgccgc cgcgcgcgtc gagcagggcg gcatcgatgc
     1681 ctggttcgcg ctcgagccac gggagcttct ggcggaagag gccgaacact acgagaaggt
     1741 caccgacgtc atggacgtgt gggctgattc ggggctctcg ttcgagtgcg tcggcgccga
     1801 gcgtccggag gtttccgcgc cggtcgagct ctacctcgaa ggctcggatc agcatcgcgg
     1861 ctggttccac agctcgctgc tcatgtccga agccctttac gagcgccctc cctaccgcgg
     1921 cgtgctcacg cacggcttca cggtcgatga gaagggtcgc aagatgtcga agtcgctcgg
     1981 caacgtgatc gcaccgcaga aggtgatgag tacgctcggc gccgatgtgc tgcggctgtg
     2041 ggtatcggcg accgactacg ccaacgagat cgcggtgtcg gacgagattc tgcggcgcat
     2101 ggccgattcc tatcggcgca tgcgcaacac actgcgcttc atgctcggca acctgcacgg
     2161 cttcgacccg gcgcagcatg cgctgcccgc tgcgcgcctc gtggcgctcg atcgctgggt
     2221 gctcgcgcgc gcgcacgcgc tgcaggccga ggttctcgat gcttaccgca cttacgcctt
     2281 tcacctcatc tatcagaagg tacacaactt ctgcagcgtc gatctcggca gcttctactt
     2341 ggatgtcatc aaggaccgga tgtacaccat gccggtcgca agtcccgggc gccgctccgc
     2401 gcagaccgtg atgtggcaca tcgccgagag catggtgcgc tggcttgcgc cgatcctgtc
     2461 gttcaccgcc gaagaggtgt gggggtatct gcccggtgag cgccgcgagt cggtgtttca
     2521 cgaaacctgg cacgcgctgc ccgaggtggc ggccgatggc gtcgactggg acgcgctcat
     2581 cgcgctgcgc agcgatgtca cccgcgagct cgagaaactg cgcgatgccg ggctgatcgg
     2641 cgcacctctc gatgcgcgcg tcgacgttta ctgcacggaa gaggagtatg tgcgctttgc
     2701 cgcactcggt ggcgagctga ggtttctcct catcacatcg gaagcccagg tacaccgcgc
     2761 cagtacttct cccgccgggt cggtgccggc cgcccatacc gggagcagcg gggtctggat
     2821 cctggcgcag ccgac
//
LOCUS       DATDMP010000610         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_207900, whole genome shotgun sequence.
ACCESSION   DATDMP010000610 DATDMP010000000
VERSION     DATDMP010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207900"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VI195_10735"
     CDS             <1..297
                     /locus_tag="VI195_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY6824907.1"
                     /translation="VDYGIYLFSRMQEFLGQGLPVREAYERTLRVTGASIIFTGITLA
                     IGVATWVFSPLKFQADIGIMLTFMFLVNMLAAIVLLPALAAWLIRPRAPDGAAR"
     gene            complement(307..726)
                     /gene="arfB"
                     /locus_tag="VI195_10740"
     CDS             complement(307..726)
                     /gene="arfB"
                     /locus_tag="VI195_10740"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY6824908.1"
                     /translation="MAVCAGVTIPDREIELAFVRSSGPGGQNVNKLATAVQLRFDLAG
                     SAALGPDVKMRLRNLSGRRVTADGTLLIVARTHRTQEQNRRAVEERLADLIRRALRAP
                     KPRRPTEPPRASRERRLERKARLQRTKRLRARPRWDE"
     gene            complement(745..2109)
                     /locus_tag="VI195_10745"
     CDS             complement(745..2109)
                     /locus_tag="VI195_10745"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY6824909.1"
                     /translation="MDSANSAASGVSLALTSPQLWAEALVIVIAVGVALAIARTTRAR
                     RARAAAAGQAPDWLERLAEVIVMLAPMLAALAVLLIGHATLAASGRSSALLDGAAQLA
                     AAVVLVRLGVYILGRLLGPGSWLQSREGGITLVLSVIVALALLGWLDAIESVLNRINV
                     VPGKSQFSLWALLKGLVVVTAFVVVTSLVARAIERHVMQLEQLAVSTRVGVSKFSYFL
                     LVGLGVLLGINAAGVEVTTLNVLTGAIGLGLGFGLQSVASNFVSGFVLLMDKSIKPGD
                     VISFTAHDGSSTGNFGWVQELRGRYVVVRDRDGVDTLVPNQNLISNPFINWSYSDQRV
                     RIKLPVAVSYEDDPELALKLLVEATENHPRILRDPQPVSRLISFDDSGIRLEIRFWIA
                     DPVNGVNNVRSDVNRAIWRIFRAHGVTIPFPQREVRIIQGRAPRPPASPGAEPTPGQD
                     RAAD"
     gene            complement(2109..2333)
                     /locus_tag="VI195_10750"
     CDS             complement(2109..2333)
                     /locus_tag="VI195_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY6824910.1"
                     /translation="MAEIEDNVDRELFRNVEKLRQLRIEHRDLDEIISRLSLDLHVNE
                     LQLKRLKKRKLMLKDQIARLESQLIPDLNA"
BASE COUNT          369 a          879 c          909 g          378 t
ORIGIN      
        1 gtcgactacg gcatctatct cttcagccgc atgcaggagt tcctcggcca gggcctgccg
       61 gtgcgcgagg cgtacgagcg cacgctgcgc gtcaccgggg cgagcatcat ctttaccggc
      121 atcaccctcg ccatcggggt cgccacctgg gtgttctcgc cgctcaagtt ccaggcggac
      181 atcggcatca tgctcacctt catgttcctg gtgaacatgc tggcggcgat cgtgctgctg
      241 ccggcgctgg cggcgtggct gatccgcccg cgggcgccgg acggcgcagc tcgctaggcc
      301 cgctcgtcac tcgtcccagc gggggcgggc gcgcagccgc ttggtgcgct gcaggcgtgc
      361 cttgcgctcg aggcgccgct cgcgcgaggc gcggggcggc tcggtggggc ggcgcggctt
      421 cggcgcgcgc agggcgcgcc ggatgagatc ggcgaggcgc tcctcgaccg cccgccggtt
      481 ctgctcctgg gtgcggtgag tacgcgcgac gatcagcagc gtgccgtcgg cggtgacccg
      541 ccggccgctt aagttccgca gccgcatctt cacgtccggc ccgagcgcgg cgctgccggc
      601 gagatcgaag cgcagctgca ccgcggtcgc cagcttgttg acattctggc cgcccggacc
      661 ggacgagcgc acgaaggcga gctcgatctc cctgtcggga atcgtcacac ccgcgcacac
      721 cgccagcggc acggccggcg acgctcagtc agcggcccgg tcctgcccgg gcgtaggctc
      781 ggcaccggga ctcgcgggcg gccgcggtgc acgcccctgg atgatccgca cctcgcgctg
      841 cgggaagggg atagtgacgc cgtgggcgcg gaagatgcgc cagatggcac gattgacgtc
      901 ggagcgcacg ttgttgacgc cgttcacggg gtcggcgatc cagaagcgga tctcgaggcg
      961 tatgccgctg tcatcgaagc tgatcaggcg cgacaccggc tgcggatcgc gcaggatgcg
     1021 cgggtggttc tcggtggcct ccaccagcag cttgagcgcc agctcgggat cgtcctcgta
     1081 gctcaccgct accggcagct tgatgcgcac gcgctgatcg gagtagctcc agttgatgaa
     1141 ggggttgctg atcaggttct ggttcggcac cagggtgtcg accccgtcgc ggtcgcgcac
     1201 caccacgtag cggccgcgca gctcctgcac ccagccgaag ttgccggtgc tcgagccgtc
     1261 gtgcgcggtg aagctgatca cgtcgccggg cttgatggac ttgtccatga gcagcacgaa
     1321 gccgctgacg aaattgctgg cgaccgactg caggccgaag ccgagcccca ggccgatggc
     1381 gccggtgagg acgttgagcg tcgttacctc gacgccggcg gcgttgatgc cgagcaggac
     1441 cccgagcccg accagcagga agtagctgaa cttcgagacc ccgacccggg tcgagaccgc
     1501 cagctgctcg agctgcatga cgtgccgctc gatggcgcgg gcgacgaggc tggtcaccac
     1561 cacgaaggcg gtcaccacca ccagtccctt gagcagcgcc cacaggctga actggctctt
     1621 gccggggacg acattgatgc gattgagcac cgattcgatc gcgtccagcc agccgagcaa
     1681 tgccagcgcc acgatcaccg acagcacgag cgtgattccg ccctcgcggc tctgcagcca
     1741 cgacccgggt ccgagcagcc ggccgaggat gtacacgccg aggcgcacca gcacgacggc
     1801 ggcggccagc tgcgccgcgc cgtcgagcag cgcgctcgag cgccccgacg ccgcgagcgt
     1861 ggcgtgcccg atcagcagca cggcgagcgc cgccagcatc ggcgccagca tcacgatgac
     1921 ctcggcgagg cgctccagcc agtccggcgc ctgcccggcg gccgcggcgc gcgcgcgccg
     1981 cgcgcgcgtc gtgcgggcga tcgccagcgc cacgcccacc gcgatcacga tcaccagtgc
     2041 ctcggcccac agctgcgggg aggtgagcgc gagggagacg ccgctcgcgg cgctgttggc
     2101 gctatccatc aggcattcag gtccgggatc agctggctct ccagacgcgc gatctggtcc
     2161 ttgagcatca gcttgcgctt cttcaggcgc ttgagttgca gctcgttgac gtgcagatcg
     2221 agcgacagac gggagatgat ctcatcgagg tcgcggtgct cgatgcgcaa ctggcgcagc
     2281 ttttccacgt tgcggaacag ctcgcggtcg acgttgtctt cgatttccgc catggcgggt
     2341 ggcccagtgt tggcgatggg cgttggacgg cctggagctg ccggcggcgg ccgccaagac
     2401 tagggagctt agccgagcgg cgcgccgagt gccacggccc gcggggtgag agccgggcgg
     2461 ggcgtgaaac cggccgtcag cgaccccggc cccgtggtgc gtgcccgggc cggcccccct
     2521 tgccgccgcg gggcg
//
LOCUS       DATDMP010000611         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1430852, whole genome shotgun sequence.
ACCESSION   DATDMP010000611 DATDMP010000000
VERSION     DATDMP010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430852"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VI195_10755"
     CDS             complement(<1..272)
                     /locus_tag="VI195_10755"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824911.1"
                     /translation="MHAPPAAEDNRLLRSIPAARVALIERITRAAPGPGRRNVLRQRF
                     LRTYFHGVAEEDLAERSPRQLARAALAHLEFGTRRAPGRSLVRVFNP"
     gene            499..1551
                     /gene="hppD"
                     /locus_tag="VI195_10760"
     CDS             499..1551
                     /gene="hppD"
                     /locus_tag="VI195_10760"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY6824912.1"
                     /translation="MSGLADNPMGTDGFEFVEYTAPDPQLLRALFERLGFPAVARHRS
                     KNVTLHRQGEINFIINAEPQSFAQSFARLHGPSACAMAFRVHDAAKAYSRALALGAKP
                     GPQSAGPMELNIPSIEGIGGSLIYLVDLYGERSIYDVDFRPVTHSAPAEGAGLTCIDH
                     LTHNVGRGRMDVWAGFYQKLFNFREIRYFDIEGKLTGLLSRAMTSPCGKIRIPINESQ
                     DDKSQIEEYLREYQGEGIQHIALATADIYRTVDVLRHQGVNFQDTPETYYEAVAARVP
                     GHRESLAELRSRGILIDGNPAKGEGVLLQIFTRNVIGPIFFEIIQRKGNEGFGEGNFR
                     ALFESIERDQIERGVI"
     gene            complement(1560..>2313)
                     /gene="zapE"
                     /locus_tag="VI195_10765"
     CDS             complement(1560..>2313)
                     /gene="zapE"
                     /locus_tag="VI195_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY6824913.1"
                     /translation="PLEHLARAIGRDTRVLCFDELYVTDIADAMILGGLFGALFRRGV
                     TLVATSNVPPQQLYQGGLQRERFLPAIALLERHLQVVRLGGSTDYRLRQLTQAGIYLP
                     AGSADTQARLQSLFERLADQDRHEDGTIEIAGRRIQVTRTGAAVVWFDFAALCATARS
                     QDDYIEIAREFGSVIVSDVPVFDAQREDEARRFIALIDEFYDRNVNLIVSAAAPPQAL
                     YRGERLAAQFARTASRLTEMQSEEYLVREHRP"
BASE COUNT          401 a          763 c          775 g          374 t
ORIGIN      
        1 ggattgaaga cgcgcaccaa cgagcgtccg ggcgcgcggc gcgtgccgaa ctccagatgc
       61 gcgagggcgg cccgcgccag ctgccgcggc gagcgttccg ccagatcctc ctcggccacg
      121 ccgtgaaaat aagtgcgcag gaaacgctgg cgcagcacat tgcgccggcc aggcccggga
      181 gcggcgcgcg tgatgcgttc gatgagcgcc acccgggcgg cgggaatgct gcgcagcagg
      241 cggttgtctt cggcagcggg tggcgcgtgc attagcgatc gtcctggtcg cggggcgtgc
      301 ggtgtcgcgt gcgcgtcgcg catgttgtac ggtccacggt cgcggctcgc gaggtcgtgg
      361 cgcgcgatta tacatgccac cggcaccgca ccgcccgcgc cagccctcgc gcggggcaac
      421 ggcggggtgc gtgtccgcgt gcgcaggccc ccgcgtgccg gacggctata atgcttgcgg
      481 cgtaaccaag gtgagctcat gagtggactt gctgacaacc cgatgggcac ggacggcttc
      541 gagttcgtcg agtacaccgc cccggacccg caattgctgc gcgcgctgtt cgaacgcctc
      601 ggttttcccg cagtggcgcg gcaccgttcg aagaacgtga ccctgcaccg tcagggcgag
      661 atcaatttca tcatcaacgc cgagccgcag tccttcgccc agtcgtttgc ccggctacac
      721 ggcccctcgg cctgcgccat ggcgtttcgg gtgcatgacg cggccaaggc atattcccgg
      781 gcactcgctc tcggcgccaa gcccggaccg cagagcgccg gcccgatgga gctgaacatt
      841 ccctccatcg agggaatcgg cggctcgctc atctatctgg tggatctgta cggtgagcgc
      901 tcgatctacg atgtcgactt tcgcccggtg acgcacagcg cacccgccga gggggcgggg
      961 ctcacgtgta tcgaccacct tacccataat gtcggccgtg gccgcatgga tgtgtgggcg
     1021 ggcttctacc agaaactgtt caacttccgt gagatccgtt acttcgacat cgaggggaag
     1081 ctcaccggac tgctgtcacg cgccatgacc agcccgtgcg gcaagatccg catcccgatc
     1141 aacgagtcgc aggacgacaa gagccagatc gaggagtacc tgcgcgaata tcagggcgag
     1201 ggcatccagc acatcgcact cgccaccgcc gacatctacc gcacggtcga tgtgctgcgc
     1261 catcagggtg tgaacttcca ggacacgccc gagacctatt acgaggccgt ggcggcgcgc
     1321 gtgcccgggc accgcgagtc gcttgccgag ctgcgctcgc gcggcatcct gatcgacggc
     1381 aacccggcga agggcgaagg cgtgctgctg cagatcttca cgcgcaacgt catcggtccc
     1441 attttcttcg agatcatcca gcgcaagggc aacgaaggtt tcggcgaagg aaacttcagg
     1501 gcgctgttcg agtcgatcga gcgcgaccag atcgagcgcg gggtgatcta acgccggact
     1561 cagggccggt gctcgcgcac caggtactcc tcgctctgca tctccgtcag acggctggca
     1621 gtacgcgcaa actgcgcagc gagtcgctcg ccgcgataaa gcgcttgcgg cggcgccgcc
     1681 gccgagacga tgagattgac gttgcggtcg tagaactcat cgatcagggc gatgaaacgc
     1741 cgtgcctcgt cctcgcgctg tgcatcgaac accggcacgt cggagacgat cacggaaccg
     1801 aactcgcggg cgatttcgat gtagtcgtcc tggctgcgcg cggtggcgca gagcgcagca
     1861 aaatcgaacc acaccaccgc ggcgcccgtc cgggtcacct ggatgcggcg gcctgcgatc
     1921 tcgatggtgc cgtcctcgtg ccgatcttga tcggcgagac gctcgaagag cgattgcagc
     1981 ctcgcttgcg tatccgcgct ccccgcaggc aggtagatac cggcctgggt gagctggcgc
     2041 agccggtaat cggtcgagcc gccgagacgc actacttgca ggtgccgctc gagcagcgcg
     2101 atcgccggca ggaagcgctc acgttgcagt cccccctggt aaagctgctg cggcggcacg
     2161 ttggaagttg ccaccagtgt gacaccgcgc cggaacagcg cgccgaacag cccgccgagg
     2221 atcatggcat cggcgatgtc ggtcacgtac agctcatcga agcacagcac gcgggtgtcg
     2281 cggccgatgg cgcgcgccag gtgctcgagc ggg
//
LOCUS       DATDMP010000612         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_652818, whole genome shotgun sequence.
ACCESSION   DATDMP010000612 DATDMP010000000
VERSION     DATDMP010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652818"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..>2119
                     /locus_tag="VI195_10770"
     CDS             171..>2119
                     /locus_tag="VI195_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6824914.1"
                     /translation="MSNETLLGVLVALPFLGSFAALALPRNARNAEASLAAIVALAGL
                     LLTITLHGPPAAGSAVRTGLPWLPGYGLEFQLRMDGLAWMFSLMVTGIGFLVVLYARY
                     YLSPRDPIPRFYSLFLAFMGCMLGAVLSGNLVQLVFFWEATSLVSFLLIGYWHQNPAA
                     RDGARIALIVTSAGGLCLLAGVLLLGQIAGSYELDAVLASGNRIRAHPHYVPALVLIA
                     LGALTKSAQFPFHFWLPQAMAAPTPVSAYLHSATMVKLGVFLLARLWPALAGTDAWIW
                     VVGTAGLITLVLGAYSAIFQHDLKGLLAYSTISHLGLITVLLGLNSPLALVAALFHIM
                     NHATFKASLFMAAGIIDHETGTRDIRRLNGLYRFMPVTATLALVSAAAMAGVPLLNGF
                     LSKEMFFAEALSVEGPLPALNRALPYVAVAWGVFSVAYSLRFIHGVFFGAPAYPREPH
                     EPPRWMRFPMDLLVLACVLVGVVPALTVAPILDAAVVAVLGSDAPAYSLAIWHGVTAP
                     FIMSLVALAGGALLYLALKRYLARGLDATPLLPPVEGRRVFDAVMVTLSWRWARLLAR
                     SLGTRRLQPQLRWIVIAALIAALWPLYRRGLQWGPLPLTRIDPAFLLLWIGGAACALG
                     AAWQAKFHRLAAIVLAGGAGLVVCLT"
BASE COUNT          250 a          745 c          697 g          427 t
ORIGIN      
        1 cgtcgagcgc cagcgcaccg cgggggcgga cctggtggtg ctgctgaaga gggctcgatc
       61 gctcggcgaa tcgctgttgc tcggcagcgt cacgcgccac gtgctcgccg atgtcgcgag
      121 cgacgtcctg ctcctcaacg aagccaagcc ggtccgataa cctgacgacg ttgtcgaacg
      181 aaacgcttct cggcgtgctg gtggcgttgc cgtttcttgg gagcttcgcc gcgctcgcgc
      241 tgccgcgcaa tgcgcgaaac gccgaggcgt cgctcgctgc gatcgtggca ctcgccgggc
      301 tcttgctcac gatcacattg catgggccgc cggcagcggg ctccgccgtc aggacgggtc
      361 tgccctggct gccgggctac ggcctcgaat tccagctgcg catggacggg ctcgcctgga
      421 tgttcagcct gatggtcacc ggcatcggct tcctggtcgt cctttatgcg cgctattacc
      481 tgtcgccgcg ggatccgatc ccgcgcttct attcgctgtt cctcgcgttc atgggctgca
      541 tgctgggtgc ggtgctgtcc ggcaacctgg tgcagctggt gttcttctgg gaagcgacca
      601 gcctggtctc gtttctgctc atcggctatt ggcatcaaaa tccggccgcg cgcgacggcg
      661 cgcgcatcgc gctgatcgtc acttccgcgg gcggcctgtg cctcctggca ggcgtgctgc
      721 ttctcgggca gatcgccggc agctacgagc tcgatgcggt actggcgagc ggcaatcgca
      781 tccgcgcgca cccgcactat gtcccggcgc tggtgctcat cgccctcggc gcgctgacca
      841 agagcgcgca gttcccgttc catttctggc tcccgcaagc gatggcggcg ccgacgccgg
      901 tgtccgccta tttgcactcc gcgaccatgg tcaagctcgg cgttttcctg ctggcacggc
      961 tgtggccggc gctggcgggc accgacgcgt ggatctgggt ggtcggcacc gccggcctca
     1021 tcacgctcgt gctgggcgcc tacagcgcca tcttccagca cgacctgaag ggactgctcg
     1081 cctattcgac catcagccac ctgggcctta tcaccgtgct cctcgggctc aacagccctt
     1141 tggcgctcgt ggccgcgctc ttccacatca tgaatcacgc gaccttcaag gcgagccttt
     1201 tcatggccgc cggcatcatc gaccacgaga ccggcacgcg cgacatccgc cggctgaacg
     1261 gcctatatcg cttcatgccg gtgaccgcaa cgctcgcgct cgtgtcggcg gcggcgatgg
     1321 ctggcgtgcc gctcctgaac ggctttctct ccaaggagat gttcttcgcc gaagcgctca
     1381 gcgtagaagg gccgctgccg gcgctcaacc gggcgctacc ttatgtcgcc gtggcctggg
     1441 gggtcttcag cgttgcgtat tcactgcgct tcatccacgg cgtgttcttc ggcgcgccgg
     1501 cgtatccgcg cgagccgcac gagccgcctc gctggatgcg cttcccgatg gatcttctgg
     1561 tgctcgcttg cgtgctggtg ggcgtcgtgc cggcgctgac cgtggcgccg atcctcgatg
     1621 cggccgtcgt cgctgtgctc ggcagcgacg cgcctgcgta cagtctcgcg atctggcatg
     1681 gcgtgacggc gcccttcatc atgagccttg tcgccctcgc gggcggcgcg ctcctctatc
     1741 ttgcgctaaa gcgctacctg gcacgagggc ttgacgccac gccgctcctt ccgccggtag
     1801 agggacgtcg ggtgttcgac gccgtgatgg tgacgctgtc ctggcgctgg gcccgcttgc
     1861 tggcccgctc gctcggcacg cggcggctgc agccgcagct gcgctggatc gtcatcgcgg
     1921 cgttgattgc cgcgctctgg ccgctctacc ggcgcggcct gcaatggggg ccgctgccgc
     1981 tcacccggat cgatcccgcg ttcctgctcc tatggatcgg cggcgccgcc tgcgcgctcg
     2041 gcgcggcatg gcaggccaag ttccaccggc tcgccgccat cgtgctcgcg ggcggggcgg
     2101 gccttgtcgt ctgccttac
//
LOCUS       DATDMP010000613         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_764261, whole genome shotgun sequence.
ACCESSION   DATDMP010000613 DATDMP010000000
VERSION     DATDMP010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764261"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VI195_10775"
     CDS             complement(<1..307)
                     /locus_tag="VI195_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6824915.1"
                     /translation="MTGRLQVVATPIGNLGDLSDRARAALAGADVIAAEDTRHTAALL
                     KASGIAKPMISLHEHNEARRVPALLERLALGECVALVSDAGTPLLSDPGYELVRAAAA
                     "
     gene            390..1952
                     /locus_tag="VI195_10780"
     CDS             390..1952
                     /locus_tag="VI195_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY6824916.1"
                     /translation="MYIRPLVRLVLIVLAAASLTACPSLSQRGELPPSLDRAQELERA
                     GDRAGAARVYEQLAVQNSGTDRTGFLLLAARDYLAAQRPDEAARVLGGAQGPFSAPQL
                     TEQALLNAQLALARGQPQEALRLLNAIAVPTAAEQAARYRELHANATALAAAHLGTAR
                     PVPPGGAGAHIGLLLPVSGRTASAAISVRDGFMTAYYQLPANERPRVRIYDTGSTSVA
                     DALTQAVGAGADIIVGPLTREEVTAAAEFPGLRVPVLALNFLPPERPAPPQFYQYALS
                     PEDEARLVARRVLDDHHRRGVALVPAGEWGTRVLAAFRQELQAGGGDLLATGQIESGR
                     TDYSATITEVLRISDSTARYHRLESILGTKLQFEPRRRNDIEFIFAPAPANLERLLRP
                     QLRFHFAGDIPTYATSDAFEPDARANEDLEGLMFPDMPWMLGGELADAVHAATREAWP
                     AGSPHRGRLFAFGFDAFRLTQALRHPGVNGTISVAGLTGRLSLDAQRHVRRELGWAQL
                     HDGELRLLPANF"
     gene            1968..2339
                     /locus_tag="VI195_10785"
     CDS             1968..2339
                     /locus_tag="VI195_10785"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY6824917.1"
                     /translation="MLTARQQSGRRAEQLAAEFLRARGCEVLQCNYRRRLGELDIVAH
                     AQGTLIVVEVRTRASGAFGGAAASVDRRKQRRITRAAQQLLQQRSDLATLPVRFDVIV
                     VSDPYGPTPAIEWIEHAFEAC"
BASE COUNT          369 a          890 c          880 g          382 t
ORIGIN      
        1 cggctgcggc agcgcgcacc aactcatagc ccggatcgga caacaacggt gtgccggcat
       61 cgctcaccag cgccacgcac tctccgagcg cgagtcgctc gagcagcgcc ggcacacggc
      121 gcgcctcgtt gtgctcgtgc agtgagatca tgggtttcgc gatgccgctc gccttgagca
      181 gcgctgcggt gtgccgcgta tcctctgcgg cgatgacatc ggcgccggcg agcgccgcac
      241 gcgcccggtc gctcaggtcg ccgaggttac cgatcggtgt ggcgacgacc tgcagccgcc
      301 ctgtcattct ggccactata atcgcgcctc acaccgcttg ggatcgcggg catcggcatc
      361 gtgcccgcca gcgtgaggct cttcaaccga tgtacattcg accgctcgtg cgcctcgtgc
      421 tgatcgtgct cgcagcagca tccctcactg cctgtccgag cctcagccag cgcggcgagc
      481 tgccgccttc gctcgatcgt gcacaggagc tcgagcgcgc gggcgaccgc gcaggcgctg
      541 cacgggtcta cgaacagctg gcggtacaga acagcggcac cgatcgcacc ggcttcctgc
      601 tgctcgcggc acgcgattat ctggccgcgc agcggcccga tgaggcggct cgcgtgctcg
      661 gtggcgcgca gggcccgttc tccgcgccac agctcaccga gcaggcgctg ctcaatgcgc
      721 agctggcgct cgcccggggc cagccgcagg aggcactgcg cctgctgaac gcgatcgcgg
      781 tccccacggc cgccgagcag gcggcgcgct accgcgagct gcacgcgaac gccaccgctc
      841 tcgccgccgc gcacctgggg accgcgcgcc ccgtgccgcc agggggtgcc ggagcgcaca
      901 tcgggctgct gctgcccgtt agcggtcgca ccgcttcggc cgcgatcagc gtgcgcgatg
      961 gcttcatgac cgcctactac cagctgccgg cgaacgagcg gccgcgggtg cggatttacg
     1021 ataccggctc gacgagcgtt gcggatgcgc tcacgcaggc ggtgggcgcg ggcgcggaca
     1081 tcatcgtcgg gcccttgacg cgcgaagagg tcacggccgc ggccgagttt cccggactgc
     1141 gtgtgccggt gctggcgctg aattttctgc cgccggagcg cccggcgccg ccgcagttct
     1201 accagtatgc gttgtccccg gaggacgagg cgcggctggt ggcgcggcgc gtgctcgatg
     1261 atcaccaccg ccgcggcgta gcgctggtgc cggccgggga gtggggcacg cgggtgctgg
     1321 cggcgttccg ccaggaactg caggccggcg gcggggatct gctcgccacc gggcagatcg
     1381 agagcggccg caccgattac tccgccacca tcaccgaggt gctgcgcatc agcgacagca
     1441 ccgcgcgcta ccatcgcctg gaatcgatcc tcggcaccaa gctgcagttc gagccgcgcc
     1501 gccgcaacga tatcgagttc atctttgcgc cggcaccggc caatctcgag cggctgctgc
     1561 ggccgcaact gcgatttcac ttcgccgggg acatacccac gtacgcgact tccgacgcct
     1621 tcgagcccga cgcgcgcgcc aacgaggacc tcgaggggct catgtttccc gacatgccgt
     1681 ggatgctcgg cggtgagctc gccgatgccg tgcacgccgc cacccgcgag gcctggccgg
     1741 caggcagtcc gcatcgcggg cggctgtttg ccttcggctt cgacgccttt cgcctcacgc
     1801 aggcgctgcg ccacccgggt gtgaacggca caatcagcgt cgcgggactc accgggcgac
     1861 tcagcctcga tgcgcagcgg cacgtgcgcc gtgagctcgg ctgggcgcag ttgcacgacg
     1921 gcgagctgcg gctgctgcct gccaacttct aacatccaac acgttacatg ctgaccgcgc
     1981 ggcagcagag tggacgacgc gccgagcagc tcgcggcgga gtttctgcgc gcccgcggct
     2041 gcgaagttct gcagtgcaat taccgccgcc gcctggggga gctcgacatc gtggcgcatg
     2101 cgcaggggac gttgatcgtg gtcgaggtgc gtacccgcgc gagcggcgcc ttcggtggcg
     2161 ccgccgcgag cgtcgaccgt cgcaaacagc ggcgcatcac gcgtgcggcg cagcaactgc
     2221 tgcagcagcg cagcgacctg gcaacgttgc cggtgcgttt cgatgtcatc gtggtgagcg
     2281 atccgtacgg gcccacaccc gccatcgagt ggatcgagca tgccttcgag gcgtgctgac
     2341 agctggagtc cggcgctgcc ggcgggccga gcagtagcgg gtcgagcatg gcagagccga
     2401 gcaccggacg gttgagcacc ggccggtcga gcatcggacg gtgcccggac cgatagtgag
     2461 ccgtttggtt aactatcggg aaaacgtgta gccatgcggg ccgacccggc cgaaggcgcg
     2521 c
//
LOCUS       DATDMP010000614         4464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1874398, whole genome shotgun sequence.
ACCESSION   DATDMP010000614 DATDMP010000000
VERSION     DATDMP010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874398"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VI195_10790"
     CDS             <1..697
                     /locus_tag="VI195_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY6824918.1"
                     /translation="LADAITGLYGSQGILGALVQRGRTGRGCHLEVSMLEAMMHFAVE
                     PFAAFFALGQAPTSADRPRLAQAYILHTADARLLAIHLSSLEKFWEGLIVALEAPELA
                     RDARFNPRQRRIAEYESLRQELDARFARQPLAHWQQRLRAQDVPHAPVNRIDEVVHDP
                     QVEHLGIIVPVEAPQQASHAVRPAFQFDGVRARSVRAAPLLDEHGKAIRAALAHAHAW
                     PTVQTPAAVAAPG"
     gene            723..1784
                     /locus_tag="VI195_10795"
     CDS             723..1784
                     /locus_tag="VI195_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY6824919.1"
                     /translation="MLFPTMLVGSYPQPDWLIDRERLAGRFPPRVRARELWRVPEPYL
                     AEAQDDATVMAIKAQEDAGLDYITDGEIRRESYSNRFATALEGIDLDNPGTALDRSGH
                     PNPVPRVVGPIKRRNAVGVRDLEFLRRHTQRRVKATVPGPFTMAQQAQIDYYGGSREH
                     AAMDYAAAVNAEIRDLFSAGADMVQIDEPYMQARPQEARAFGLKALNRALEGVSGTTA
                     VHICFGYAAIIHVRPSGYSFLPELAECRCQQVSIETAQSSLDCAVLKALPEKKIILGV
                     IDLETHTVESPELVASRIRRALPYVEAGSLLVAPDCGMKYLPREVAFGKLQAMVAGAR
                     IVRRELEGSAAAADTRANS"
     gene            1826..4150
                     /locus_tag="VI195_10800"
     CDS             1826..4150
                     /locus_tag="VI195_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6824920.1"
                     /translation="MFTALRRTVPLLTAALGVLTLTAPAWGQTQSKTQAQANEPTLEE
                     VVVTARKREEALLDVPVTVDAFTQQTIQSAGIEAPRDFVALVPNMTLVEVQNVGNSFI
                     TIRGISQARNSEPSVAVLVDGVLEPNPYEFDQELNDVTSIQVLKGPQGALYGRNAIGG
                     AIIINTADPSDHFEGAAKVGIDNGVSEKAQVAVGGPIDSAGTLKYRASLNFYNTDGYL
                     ENTALDRKADPYRDYSGRLRLLWKPSDQLSADLRFFGDHVETTAYYYIIPRKVEANPF
                     SSFPPSTFNPDGTPPNANDITSPIQTNNLGTDTRDISDIALKLDYAVGPGTFTSITDW
                     DHTKEIDTGDAYDFRPITTSIFYNCPSCLGIPPPYPTPAQGGPFDLSQSQFIDVTTWS
                     QELRFTSNKVGAFAWIAGAYFIHTERFISTDNIVDRGEGVPRVYHDPIVDPTNPFATN
                     TNATFLADSQINNAWAVFADATYEFTRQLELDVALRYDEDQRQNTTDTPNQFLPAATK
                     PDAFTGLVRKETFDAVQPKFTLRYKPTDNLTFYGGWSRGFRSGGFNQTGVGAVAAAAG
                     QKGVHDIFQAEIADTWEIGMKSEFLDRRLAVNAALFDTTSTNGYFFYFDATTSTQNLG
                     NLDARYKGGELELTAIATNWLDLYASFGYTDGKITNMEDPTVIGNRPPLLTKDTINEG
                     YQVHQPLGSGVTGVLRLDYQMIGTTWWDPYNVTSRDPVDLLDLRAGLEGERWSVTAWS
                     KNLTNKLYNAEFSAGGFLWRALPRRYGLDFTYKF"
     gene            4166..>4464
                     /locus_tag="VI195_10805"
     CDS             4166..>4464
                     /locus_tag="VI195_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6824921.1"
                     /translation="MAPLNKLPSRLHHNAYVTKDQEKTRQFYEDVIGLPLLATWCESD
                     ELFGKVRTYVHTFYGIGDGGALAFFQFADPADQEEFGPKMPPTPFHHIALRVDAET"
BASE COUNT          842 a         1470 c         1376 g          776 t
ORIGIN      
        1 actcgccgat gccatcaccg gtctctacgg ctcgcagggg attctcggcg cgctggtaca
       61 gcgagggcgc accgggcgtg gctgccacct cgaggtgtcg atgctcgagg cgatgatgca
      121 ttttgccgtc gagcccttcg cggctttctt cgcgctcggg caggccccga cctcagcgga
      181 ccggccgcgt cttgcgcagg cctacatcct gcataccgcg gatgcgcggt tgcttgccat
      241 ccacctctcc tcgctcgaga aattctggga ggggctgatc gtggcgctcg aggcgccgga
      301 actcgcacgt gacgcgaggt tcaatccccg tcaacgacgt attgccgagt acgagagcct
      361 gcgtcaggag ctcgacgcac gcttcgctcg ccaaccgctt gcgcactggc agcagcgctt
      421 gcgggctcag gacgtgccgc acgcgcccgt caatcggatc gacgaggtgg tgcacgaccc
      481 acaagtggag cacctcggaa tcatcgtgcc ggtcgaggca ccgcagcagg cgtcgcacgc
      541 agtgcgcccg gcatttcagt tcgatggcgt gcgtgcccgc tcggtgcggg cagcgccgct
      601 gctcgatgag cacggtaagg cgatccgcgc ggccttggcg catgcccacg cctggccgac
      661 ggtgcagacg ccagcggcgg ttgccgctcc cggctgaggc aacgcacgag ggggtggtcc
      721 gcatgctctt tcccaccatg ctggtcggta gttacccgca acccgattgg ctcatcgacc
      781 gcgagcggct ggcagggcgc ttccccccgc gggtgcgggc ccgggagtta tggcgtgtgc
      841 cggagccgta tctcgccgaa gcgcaggacg atgcgacggt gatggcgatc aaggcacagg
      901 aagatgccgg actcgactac atcaccgacg gcgagatccg ccgcgagagc tactccaatc
      961 gctttgccac ggcgctcgag gggatcgacc tggacaatcc gggcaccgcc ttggatcgca
     1021 gcgggcatcc gaatccggtg ccgcgcgtgg tcgggccgat caaacgccgc aacgccgtcg
     1081 gcgtccgcga cctcgagttc ctgcgccgcc acacgcagcg acgggtcaag gcgacggtgc
     1141 ccggaccatt caccatggcg cagcaggcgc agatcgacta ctacgggggc agccgcgagc
     1201 acgctgccat ggactatgct gcggcagtca atgcggagat ccgcgacctg ttcagcgccg
     1261 gggctgacat ggtgcagatc gatgagccgt acatgcaggc acgcccgcag gaggcgcgcg
     1321 ccttcggctt gaaggccctg aaccgcgcgc tcgagggcgt gagcggcacg acggcggtac
     1381 acatctgctt cggctacgcc gccatcattc acgtgcggcc ctccggctac tcgtttctgc
     1441 cggagctcgc cgagtgtcgc tgccaacagg tatcgatcga gaccgcgcag tccagcctcg
     1501 actgtgcggt gctcaaggcg ctgccggaga agaagatcat tctcggtgtc atcgatctgg
     1561 aaacccacac cgtcgagtcg ccggagctcg tcgcaagccg tatccggcgc gcgctgccct
     1621 acgtggaagc cggcagtctg ctggtggcgc cggactgcgg catgaagtac ctgccgcgcg
     1681 aggttgcctt cggcaagctc caggccatgg tcgccggcgc ccgcatagtg cgtcgcgagc
     1741 tcgagggctc agccgcagct gcggatacgc gtgcgaattc ctgaccatca cgatgactat
     1801 cacaagcaga agagaggggt ctcacatgtt cactgcgttg cgccgcactg ttccgctact
     1861 gactgccgcg ctcggcgtcc tgaccctcac tgcgcccgcc tgggggcaga ctcagagcaa
     1921 gactcaggct caggccaacg agccgacgct ggaggaagtg gtggtcaccg cccgcaagcg
     1981 cgaagaagcg ctgctcgatg tgccggtgac cgtggatgcc tttacgcagc agacgatcca
     2041 gtcggcgggg attgaagcac cgcgcgattt cgtcgcgctg gtgccgaata tgacgctggt
     2101 cgaggtgcag aacgtcggta actcgttcat caccatccgc ggcatctcgc aggcgcgcaa
     2161 cagcgagcca tcggtcgcgg tactggtcga tggcgtgctc gagcccaatc cctacgagtt
     2221 cgatcaggag ctgaacgatg tcacctcgat ccaggtgctg aagggtccgc agggcgctct
     2281 gtacggtcgc aacgccatcg gcggcgcgat catcattaac accgcagatc cttccgatca
     2341 cttcgaaggc gcggccaagg tcggtatcga caacggtgtg tccgagaagg cccaggtggc
     2401 ggtcggcggg ccgatcgact cggccgggac gctcaagtac cgcgcctcac tcaacttcta
     2461 caacactgac ggctacctgg agaacaccgc cctggatcgc aaggcagatc cctaccgtga
     2521 ttactccgga cggctgcggc tgctgtggaa accctccgac cagttgagcg ccgatctgcg
     2581 gtttttcggt gatcacgtag agacgaccgc gtactactac atcattcccc ggaaggtgga
     2641 ggcgaacccc ttcagcagtt ttccgccctc aacctttaac ccggacggca cgccgccgaa
     2701 cgccaacgac atcaccagtc cgatccagac caacaatctc ggcacggaca cgcgcgacat
     2761 cagcgacatc gcgctcaagc tcgattacgc cgtcgggccc ggcacgttta cctcgatcac
     2821 ggactgggac cataccaagg aaatcgatac cggtgacgcc tacgatttcc ggcctattac
     2881 tacgtccatt ttctacaatt gtccgtcttg ccttggcata cctcctccgt acccaacacc
     2941 tgcccaaggc gggccgttcg atctgagcca gagccagttc atcgacgtga cgacctggag
     3001 ccaggagctg cgcttcacct ccaacaaggt cggcgccttc gcgtggatcg cgggggcgta
     3061 cttcattcat acggagcgtt tcatttcgac cgacaacatc gtcgaccggg gagaaggcgt
     3121 gcctagggtc tatcacgacc cgattgtcga tccgaccaat ccgttcgcga ccaacaccaa
     3181 cgcgacgttt ctcgccgatt cgcagatcaa caatgcctgg gccgtgttcg ccgatgcaac
     3241 ctacgagttc accaggcagc tcgaactcga tgtggcgctg cgctatgacg aggatcagcg
     3301 gcagaacacc accgatacgc ccaaccagtt cctgcctgct gcgacaaagc ccgatgcatt
     3361 caccggcttg gtgcgcaagg aaaccttcga cgcggtgcag cccaagttca cgctgcgcta
     3421 caagccgacc gacaacctga ccttctacgg tggctggagc cgcgggtttc gcagcggcgg
     3481 tttcaaccag accggagtgg gcgcggtcgc cgcggccgcg gggcagaagg gcgtacatga
     3541 catcttccag gccgagatcg ccgacacctg ggagatcggc atgaagagcg agttcctgga
     3601 ccggcggctg gcggtgaacg cggcactctt tgacaccacc tccaccaacg gctacttctt
     3661 ctacttcgat gcgacgacct cgacccagaa tctcggcaac ctcgatgccc gttacaaggg
     3721 cggcgagctc gagctgaccg ccatcgcgac gaactggctc gacctctacg ccagcttcgg
     3781 ctacaccgac ggcaagatca ccaacatgga ggatcccacg gtgatcggca accgtccgcc
     3841 gctactgacc aaggacacca tcaacgaagg ctaccaggtc caccagccgc ttggtagcgg
     3901 cgtgaccggc gtgctgcgtc tggattatca gatgatcggc accacctggt gggacccgta
     3961 taatgtgacc tcgcgtgatc cggtcgatct gctggatctg cgcgccgggc tcgagggcga
     4021 gcgctggtcg gtgactgcct ggtcgaagaa tctgaccaac aagctgtaca acgcggagtt
     4081 ctcagcgggc ggcttcctgt ggagagccct gccacgccgc tacgggctcg atttcacgta
     4141 caagttctga gaaggaggta ctcacatggc gcccctgaac aaacttccct cgcgcctgca
     4201 tcacaatgcc tatgtcacca aggaccagga aaagacgcgc cagttctacg aagatgtgat
     4261 cgggttgccg ctgctcgcca cctggtgcga atccgacgag ctgttcggca aggtgcgcac
     4321 ctacgtgcat accttctatg gaatcggcga cggcggcgcg ctggcattct ttcagtttgc
     4381 tgatccagcg gatcaggagg aattcgggcc aaagatgccg ccgaccccgt tccatcacat
     4441 tgcgctgcgg gtcgatgccg agac
//
LOCUS       DATDMP010000615         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1207745, whole genome shotgun sequence.
ACCESSION   DATDMP010000615 DATDMP010000000
VERSION     DATDMP010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207745"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VI195_10810"
     CDS             complement(<1..280)
                     /locus_tag="VI195_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6824922.1"
                     /translation="MALYLEHFGLDEPPFRITPHTDFFFDGAERGATLEALAYAVVHD
                     EGIVKVSGEVGSGKTMLCRMLMERLPAEVETIYLATPSLARDEILHALA"
     gene            complement(283..1695)
                     /locus_tag="VI195_10815"
     CDS             complement(283..1695)
                     /locus_tag="VI195_10815"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6824923.1"
                     /translation="MKPAASAPEALARIALLLVDDDPLITDTLAFALRDDYQVHACAS
                     RAGAIDVVRLLATPPPLALVDLGLPPTPHSPGEGFQLISDLLAHAPAMKIFVLSGQND
                     AANARHARTLGAWEFVAKPAEPALLKRLLARALQTPATAEAGLVGESPSLLNLKSQIA
                     QFAPALYPVLIEGESGSGKELAARSLHNLSTRAARPYLSLNCATLAPTVVEPTLFGYV
                     KGAFAGATTHKSGYFEDAQDGTLLLDEIGELPLELQAKLLRVIENGEYQRVGETQRRM
                     ARCRVLAASNRDLRSEVRKGAFRADLYHRLSVFTLTVPPLREMERDKRRLLEHFSALV
                     ATQSGQPSFELDAGAAERWQQYDFPGNVRELRNIVLRLAAKYPGQRLSNAELEAEFDL
                     ETPLPHATTQAAAALVEQALRQLERAGAFNLDETLKAWERGYIEAALRLARGNVSQAA
                     RLLGINRTTLYSRMGTTQEK"
     gene            complement(1670..>2163)
                     /locus_tag="VI195_10820"
     CDS             complement(1670..>2163)
                     /locus_tag="VI195_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6824924.1"
                     /translation="RVVPPHYLTGAEFLRALAALSGERYSEARVSALTDELELEPQAL
                     ARPVRALSKGMTQKLGLAACLSLERELYVLDEPMSGLDPVARVAVKSVLGRLNREGRT
                     LFFTSHVLADVDELCSSLAVLERAALRYRGTPQNLCTRYGEASLERAFIRCIRDEAGR
                     VRA"
BASE COUNT          363 a          724 c          736 g          340 t
ORIGIN      
        1 cggcaagggc gtgcaggatt tcatctcgcg cgagcgacgg ggtcgcgaga tagatggtct
       61 cgacctcggc gggcaggcgc tccatgagca tgcggcagag catggtcttg ccgctgccca
      121 cctcgcccga caccttgacg ataccttcgt cgtgcaccac ggcataagcc agcgcctcga
      181 gcgtcgcgcc acgctcggca ccatcgaaga agaaatcggt gtgcggcgtg atgcgaaagg
      241 gcggctcgtc gaggccgaag tgctcgaggt aaagcgccat ggctacttct cctgcgttgt
      301 gcccatacgg ctgtagagcg tagtgcggtt tatgccaagg agccgcgcgg cctggctgac
      361 attgccccgc gcgagcctga gagccgcctc gatatagccg cgctcccagg ctttgagcgt
      421 ctcgtccagg ttgaaagcgc ccgcgcgctc gagctggcgc agcgcctgct cgacgagggc
      481 agccgccgcc tgcgtcgtcg catgcggcag cggcgtctcg agatcgaatt ccgcctccag
      541 ctcggcgttg gagagccgct gccccgggta cttggccgca agccgaagga cgatattccg
      601 cagctcgcgt acgttgccgg gaaaatcgta ctgctgccag cgctcggcgg cgccggcatc
      661 cagctcgaag gacggctggc cggattgcgt ggcgacgagc gcgctgaagt gctcgagcag
      721 gcgccgcttg tcgcgctcca tttcgcgaag cggcggcacc gtcagcgtga ataccgagag
      781 gcgatggtag aggtcggcgc ggaacgcccc tttcctgacc tcgctccgca ggtcgcggtt
      841 ggaggcggcg agcacgcggc agcgggccat gcggcgctgc gtctccccca cccgctggta
      901 ctcgccgttc tcgatcacgc gcagcagctt cgcctgcagt tccagcggca gctcgccgat
      961 ctcatcgagg agcagcgtgc cgtcctgggc gtcctcgaag tacccggact tgtgagtcgt
     1021 ggcgccggcg aacgcccctt tcacgtagcc gaacagggtc ggctcgacca ccgtcggcgc
     1081 aagcgtggcg caattgagac tcaggtacgg ccttgcggcg cgggtcgaga ggttatggag
     1141 actgcgcgcc gcgagttcct tgccgctgcc agactcgcct tcgatcagga ccggataaag
     1201 cgccggcgcg aactgcgcaa tctgactctt cagattgaga agcgacggac tttcgccaac
     1261 caggccagct tccgcggtgg ccggcgtttg gagcgcgcgc gcgagcaagc gcttgagcaa
     1321 cgccggctcc gccggcttag caacgaactc ccaggcgccg agcgtccggg cgtggcgtgc
     1381 attggcggcg tcgttctggc ccgagaggac gaagatcttc atcgcaggcg catgggccag
     1441 cagatcgctg atcaactgaa agccttcgcc gggcgaatgc ggcgttggcg gcagccccaa
     1501 atcgaccagc gcgagcggcg gcggcgtggc aaggaggcgc accacatcga tcgcccccgc
     1561 gcgcgatgcg caggcgtgca cctggtagtc gtcgcgcagc gcgaacgcga gcgtgtcggt
     1621 gatgagcggg tcgtcgtcga ccagcagaag cgcgatgcgc gcaagcgctt caggcgcgga
     1681 cgcggccggc ttcatcgcga atacacctga tgaacgcgcg ctcgaggctc gcttctccat
     1741 agcgcgtgca aaggttctgg ggcgtgccgc gataacgcag cgccgcgcgc tccagcaccg
     1801 cgagcgagga gcaaagctcg tcgacgtccg ccagcacatg cgatgtgaaa aacagcgtcc
     1861 gcccctcccg gttcaagcgg ccgagcaccg atttcaccgc cacgcgcgcc acagggtcaa
     1921 gaccgctcat cggctcgtcg agcacgtaga gctccctctc gagactcagg cacgcggcga
     1981 gcccgagctt ttgcgtcatg cctttcgaga gagcgcgcac cgggcgtgcg agcgcctgcg
     2041 gctcgagctc cagctcatcg gtcagcgcgc tcacccgagc ctcgctgtag cgctctccgc
     2101 tcagcgcggc gagcgcgcgc agaaattcgg cgccagtcag gtagtgcggc ggaacgactc
     2161 gct
//
LOCUS       DATDMP010000616         2393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2209557, whole genome shotgun sequence.
ACCESSION   DATDMP010000616 DATDMP010000000
VERSION     DATDMP010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209557"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..826
                     /gene="lptB"
                     /locus_tag="VI195_10825"
     CDS             104..826
                     /gene="lptB"
                     /locus_tag="VI195_10825"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY6824925.1"
                     /translation="MTALKATDLAKSYRSRQVVRNLSLELDSGEVVGLLGPNGAGKTT
                     AFYMIVGLVPCDAGRIVLGEVDLTLLPMHRRAQLGLGYLPQEASVFRKLSVEDNIMAI
                     LETRNMERGAREQRLEQLLEELRIGHVRKTLGLALSGGERRRVEIARALAAEPRFMLL
                     DEPFAGVDPLSVLDIQRIIRELTQRGIGVLITDHNVRETLGVCARAYIVNQGTVIAAG
                     TAEEVLANPQVREVYLGESFRL"
     gene            872..2311
                     /locus_tag="VI195_10830"
     CDS             872..2311
                     /locus_tag="VI195_10830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY6824926.1"
                     /translation="MLKPSLQLKLGQQLTMTPQLQQAIRLLQLPALELQAHIRELLET
                     NVMLEPLEEAEAATGTFDALVTAAEQAKPAERAAESTVEVLEDGWSEHTAGPADTPWN
                     GDDDERQQEYADASGESLQDHLLWQLELAHLQPRQLAIARAIVDAISDDGYLTESLEE
                     IARTLHPELECASAEVASVLLRVQALDPAGIGARSVGECIELQLRQLDPSTPGFETAL
                     RIARHHLELVAERELSLLKRELRATEEEIASALALVRSCHPRPGATVSAGKAQYVVPD
                     VFVRRTDHGWGVEINPATLPRVRLNQSYASLIGRNASHATMRAQLQEARWLLKSLEIR
                     HETLIKVARSIVERQTAFLEHGEEYMRPMILKDIAEAVTMHESTISRVTSGKYMHTPR
                     GVFELRYFFSSQVEGADGSGTSSTAIRAKIRKLVKDEHPEAPLSDGRIAELLSAAGIP
                     VARRTVAKYREAMGIGPSNERRRTGLKQM"
BASE COUNT          439 a          781 c          805 g          368 t
ORIGIN      
        1 atcagcgcgt gcacatcacc atcacgccgc agagtgcgcc ggcaacgccc gcgggcaaga
       61 cgcacaacga gccgggcaag ccgccgccct cgaatcccaa gtcatgactg cgctcaaagc
      121 cacggacctg gccaagagct accggtcgcg ccaggtggtg cgcaatctgt cgctcgagct
      181 cgatagcgga gaagtggtgg gtctgctcgg cccgaacggc gccggcaaga ccaccgcctt
      241 ctacatgatc gtgggactgg tgccgtgcga tgcgggacgc atcgtgctcg gcgaggtgga
      301 cctgacgctg ctgccgatgc atcgccgtgc acagctcggt ctcggttacc tgccgcagga
      361 agcctccgtg tttcgcaagt tgtcggtaga ggacaacatc atggcgattc tcgagacgcg
      421 caacatggag cgcggcgcgc gtgagcagcg tctcgagcag ttgctggaag agctgcgcat
      481 cgggcacgtg cgcaagacgc tcggactggc gctctcgggc ggtgagcggc gccgcgtcga
      541 gatcgcacgg gcgctggccg ctgagccgcg cttcatgctg ctggatgagc cgttcgccgg
      601 cgtcgatccg ctgtcggtgc tcgatattca gcgcatcatc cgcgagctta cccagcgtgg
      661 catcggggtg ttgatcactg accataatgt gcgcgaaacc ctgggggtgt gtgcgcgggc
      721 ctacatcgtc aaccagggca cggtgatcgc cgccggcact gcggaagaag tccttgccaa
      781 cccccaagtc cgtgaggtgt acttgggtga gtcgttccgg ctttgaagct gaggaactgc
      841 aacggctgac atcaggacac cacaagtcac catgctcaaa ccttctctgc agctgaagct
      901 gggtcagcaa ctgaccatga ccccgcagct gcagcaggcc atccgtctgc ttcaactccc
      961 ggcactcgag ctgcaggcgc acatccgcga actgctggaa accaacgtga tgctcgagcc
     1021 gctcgaggag gccgaggccg ccaccggcac gttcgatgcg ctggtcaccg cggcggagca
     1081 ggcgaagccc gccgagcgcg ccgcggagag cacggtggaa gttctcgagg acggctggag
     1141 cgagcacacc gctggccccg cggacacgcc ctggaacggc gacgatgacg agcggcagca
     1201 ggagtacgcc gacgcctccg gggagtcgct gcaggatcac ctgttgtggc agctggagct
     1261 cgcgcatctg cagccgcggc agctggccat tgcgcgcgcc atcgtcgacg cgatcagcga
     1321 cgacggctac ctcaccgaat ctctcgagga gatcgcgcgt acgctgcatc cggagctcga
     1381 gtgtgcgagc gccgaggtcg ccagcgtgct gctgcgcgta caggcgctcg atcctgccgg
     1441 tatcggtgcg cgctcggtcg gggagtgcat cgagctgcag ttgcgccagc tcgacccctc
     1501 caccccgggc ttcgagacag cgctgcggat cgcgcgccac catctcgagc tggtggccga
     1561 gcgcgagctg tcgctgctga agcgcgagct gcgcgccacc gaggaggaga tcgcgagcgc
     1621 gctcgcgctg gtacgctcgt gccatccgcg tccgggcgcc accgtgagcg ccggcaaggc
     1681 gcagtacgtg gtgccggacg tgttcgtgcg ccgcaccgat cacggctggg gcgtggagat
     1741 caatccggcg acgttgccgc gcgtacggct caaccagagc tatgccagcc tcattggccg
     1801 caacgcgagc cacgccacca tgcgggcaca gctgcaggaa gcgcgctggc tgctgaaaag
     1861 cctggagatt cgtcacgaga cgctgatcaa ggtggcacgc tcgatcgtcg agcggcagac
     1921 ggcgttcctc gagcacggcg aggagtacat gcggccgatg atcctcaagg acatagccga
     1981 ggccgtgacc atgcacgagt cgaccatctc gcgcgtgacc tccggcaagt acatgcacac
     2041 tccgcgcggc gtgttcgagc tgcgctattt cttctcgagc caggtggagg gtgccgatgg
     2101 ctcgggcacc tcatcgacgg cgatccgcgc caagatcaga aagctcgtca aggatgagca
     2161 cccggaagct ccattgagcg atggccgcat cgccgagctg ctctcggccg cgggcattcc
     2221 ggtggcgcgg cgcaccgtag ccaagtaccg cgaggcgatg ggaatcggcc cctcgaacga
     2281 gcgccgccgc acgggactga aacagatgta agcagaagtc atacaaggag acgaggtatg
     2341 cagctcagcg tcaccggcca ccacgtcgaa gtcaccgccc cgctgcgtag cta
//
LOCUS       DATDMP010000617        13683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1764481, whole genome shotgun sequence.
ACCESSION   DATDMP010000617 DATDMP010000000
VERSION     DATDMP010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764481"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2488)
                     /locus_tag="VI195_10835"
     CDS             complement(116..2488)
                     /locus_tag="VI195_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6824927.1"
                     /translation="MKPTAAAIDEALQAPSRDSAVALIDEGNALEEQGRTAEAMARYD
                     AAAQADPHCARAHLNRGNILLAREQVDEARAAYRLAIANDPDYAAAHFNLGNLNYRTG
                     ERQHALDNYRAALGIKPDFAAALVAQGNALEDLGRTSEAIASYERALAIDPGDAGVHL
                     NLALLSMAAGRHEQAADSLRRAIDIRPDYAAAHHALGQTASSLGQLDAAEASFRRALS
                     LEPESDEILYNLALILMARSKSPEGVQLTARRLEIAPSRQIKTAFAYCAVYTRFTAND
                     AGVRAALTVAITEPWGTPYQLSRPAISLLMLDPRIASCVRIANESWPAHIPGTVLFGR
                     EGLAALASDQLLHALLQAAPVCTTEFERFLTAARHALLEAATNQPPPDSSGLAALPFY
                     AALARQCFINEYIFDCDESERAAAEACRSRLLELLDADAPPPPLLLLAVAAYFPLCTL
                     PEPARLLAADAQGPVAELLRQQVREPLEERVLRSGIECLTAIDDGVSEKVREQYEENP
                     YPRWVKLPSVRPERRLNAELRRTFPLARFVPMHDDSQPEMLIAGCGTGSQPILATHRI
                     RSVRVLAVDLSLSSISYAKRKTREMGITNIEYAQADIMKLGDITRTFDIVASIGVLHH
                     LADPFLGWRILLSRLRPGGVMHIGLYSQIARRHVAKAREFIAARGYQGTLHDIRRVRR
                     DLGALGAGDELQWLSQAPDFYSTSDCRDLLFHVQEHCLTLDQIESFLSDSGLHFLGFE
                     LDAQVLHRYRTRFTRDPAATNLHNWAHFEADNPDTFAGMYQFWVQKPVHQ"
     gene            complement(2711..3313)
                     /locus_tag="VI195_10840"
     CDS             complement(2711..3313)
                     /locus_tag="VI195_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824928.1"
                     /translation="MRALICGAALCLGACGSTTFTSTWKAPDAQRVDPVGKNIAALVI
                     VSDKKRRRDAEVYLTHELTIRGARGVAAYTLIGLDHPTVDYARTRFKEAGVEGVVIMR
                     LVGHDTHTIVDPGGFSGSAYGTFGSYYSSYGMELTYSTGSVQTDEVLTIETLIYSLTQ
                     DKLLWAGTSRTSNPEGVQSLITEVADAVASQVAKQGVVAR"
     gene            complement(3418..4053)
                     /locus_tag="VI195_10845"
     CDS             complement(3418..4053)
                     /locus_tag="VI195_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824929.1"
                     /translation="MWRAVLEQYPGMPLRRILLIALAVAGLWQGYENWRLRPVHPADG
                     AIAPDDPRQTDIEAPRVTVLGQWRLTARAHYEITARILGREDYRFDQLADLIPEDLAL
                     GWGAMSDNRVLEAFEITQGARFYSWKPKRGLPIPRQAVIEHSANTHVIPADRVVAGKL
                     QRLRVGQVVHLTGYLVNGVRQDGAYLNTSLTRSDSGPGSCEVVLVEAVEAW"
     gene            complement(4155..4445)
                     /locus_tag="VI195_10850"
     CDS             complement(4155..4445)
                     /locus_tag="VI195_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824930.1"
                     /translation="MKMAILMSALLLGGCAHTSHVQDLGNGKHAVTASANWGGYTGSR
                     EETIAQANEFCGKSRQTAAIESFEDKPGVSPKGEQTSTLNFTCTARPVLQLR"
     gene            4762..5505
                     /locus_tag="VI195_10855"
     CDS             4762..5505
                     /locus_tag="VI195_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824931.1"
                     /translation="MTMMQLIIGAALGFVVAQSGLYGIRHSLGWLQREEVSTRVRTLP
                     GHVIVGGFIRYAAPIGATAALITLAVWGVGDYLAARTARDATLANALDSAASAGTAQV
                     QGSADESSVASASAPSESASSIQSEGVDPYADPDYKVHRRVHRTGSPVTLKETLLQRS
                     EAKARSELLQDMQQHAARSQYDCEAADRAARYLKAGLDVWGFGAWQLKYFPVEGYKGA
                     TLGGCKDIPNVVDPSQLNLQSTVAQENHS"
     gene            complement(5540..6040)
                     /locus_tag="VI195_10860"
     CDS             complement(5540..6040)
                     /locus_tag="VI195_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824932.1"
                     /translation="MRILPYLRTGALCALLAPAFALAADKPVVNEKFSDLEPAIQMLR
                     QEVGKDRRAIVKANMLLTESEAARFWPIYDQYRADMSKLGDRRVKLITDYAANRTSMS
                     EDEGHRLLKEALDIDKERTELKEDYAKKFNKELSARTTARFFQIDQKLDAALDASLAA
                     RIPLIY"
     gene            complement(6222..7385)
                     /locus_tag="VI195_10865"
     CDS             complement(6222..7385)
                     /locus_tag="VI195_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY6824933.1"
                     /translation="MSGLSSLTRNVVPIDAAQHGARLAKLQGLMQQRRTGAILIESGS
                     TLEYFTGIHWWRSERTTAALVAAEGPVIVVTPAFEEPSIRETLRVPADVRPWKEDESP
                     FALLAGAWRDHGRGGALAIEPTTRYFIVEQVVAAGGATKPSVIPGDDLVRACRMVKSP
                     SELALLQAANSVTLAALRYTHRRVRAGMKATDILALMVAATEQLGGSHEFTLVLLNEA
                     SAYPHGSIKPQQVREGSVILIDTGCSVHGYQSDISRSWVYGEISARQRELWDTVKRGQ
                     ELTLETAKPGVAAGAVDRAVRAFYEQKGWRPDYALPGLSHRTGHGIGMDVHEPPYLVR
                     SDKTALQPGMCFSDEPGLYIPGEFGIRLEDCWYMSEAGPKLFTPLARSIEQPV"
     gene            7652..9511
                     /locus_tag="VI195_10870"
     CDS             7652..9511
                     /locus_tag="VI195_10870"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEY6824934.1"
                     /translation="MNIRLKVIALIGAIFAILVGAEVLVERHVVMPSFTQLERADSQT
                     AMRRIVYALDRTLTELELLATDWGNWGDTYQFVEDHNPAFVAANISNIALRQLNVNVL
                     MIVDRDGKLVEAHDLDLQSDQPLGLELTSQPALPADFPWRANLRTGTATRCLLRTSRG
                     ILLLAAAAVLDGHGNGPARGMVVIGRLLSPAVVRSLGDQAQVQLAMLPPDPNTTSERM
                     LESDSLIQVYRAFNDLYGKPVMTLRVDVPREISRRGYRAVTYASACLIAAAVIVLLLL
                     VLVLNRVILNPLAQVTRHAVALGADKDLTTRLDLKRGDEFGVLAREFDRMVERVEESR
                     MQLVDQSFQAGFAELAKGVLHNLGNAMTPISVRLASLTDRLRAAPTADAEQAFAELKQ
                     GGADAQRRADLEKFLQLACRELAATLRSSQEDLATITRQTGLIQAALAEQMRSARNEA
                     VIEPVRLTELLAQSLDIVPDSCRARLSIDADDTVRRVGVVQVARTVLRLVLQNLIINA
                     ADAVRDAGKDKGKLRVSAAIVHEADGRQLHLQCVDDGVGIPAENLERVFENGFTTKSP
                     ETNHGIGLHWCANAIGALGGRIWAASDGPGSGAALHLLVPLGARETAPAAGAA"
     gene            9480..11417
                     /locus_tag="VI195_10875"
     CDS             9480..11417
                     /locus_tag="VI195_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6824935.1"
                     /translation="MKLLPRQEPPEVLMAERNKPIRVLVVDDEPEIRDAYKQILCETE
                     VNLEMTGFHELRSRLFRKNPMEALRQAATRSTGFDTVFCQQASEAVAAVKEALARNEP
                     FAVAFIDMRMPPGADGVWAATQIRELDPALEIVICTAYSDADPGEIGGYVPPEDKLSY
                     LQKPFHPHEVRQMTIALGSKWRAERRIVRLAYFDTLTGLPNREQSRNRLIGALQAAKD
                     KNGLLAVLYLDLDNFKRVNDTLGHAVGDQLLCVVADRLRSSLRYGADSNSGGGGNRLG
                     DIARLGGDEFMVLLPNLRNTVDAGGVAERLILSLRQPIQLASNSLVVTPSVGIALYPQ
                     DGTDAETLLRNADLAMYFAKRRTPGTFAFFDVSMNASALHRFTIEEQLRGALERNEFS
                     LQYQPQFDVRSGTISGVEALLRWTNAELGAVSPLEFIPVAEETGLILAIGKWALRAAC
                     QQAQAWRAEGLPVQRMAVNVSGRQFALAEYPQEVAEILSETGLEPAMLELEITESVVM
                     ANEAWAEKAIKELKALGISLAIDDFGTGYSSFGRLRHFEVDRLKIDRSFVTSISDHSD
                     DRAIAAAIIAMSRSLHIHVTAEGVENYPQLAFLQEQECQDAQGFLLSRPLQADAAREL
                     LRRVGELADASRTQRLKIITG"
     gene            11535..12815
                     /gene="glcF"
                     /locus_tag="VI195_10880"
     CDS             11535..12815
                     /gene="glcF"
                     /locus_tag="VI195_10880"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY6824936.1"
                     /translation="MQTALADFISNSPAGRQADAILRSCVHCGFCLPACPTYQLLGDE
                     LDSPRGRIYLMKQLLEGEPVSAPTQLHLDRCLTCRACESACPSGVRYGRLLDIGRAVV
                     EERVPRPLPARGRRYLLRKIVPYTRRVRALLGLAAAVRPLLPRALRAAVPNAEARASP
                     LEQLERPSLWPPARHARRVVLLEGCVQPALAPGINPAAAQVLDRIGLSTIRVAAAGCC
                     GALSHHLAAHQESRAQARRNVDALWPHVQAGAEAVVFAASACAAMVCDYGELLSDDPS
                     YAARAARVSALARDIGPLLAAHAGALRAALAAASSAAPVPAAARVAFHAPCTLQHALR
                     ASGVVEPLLQAAGFTLTAVADGARCCGSAGTYSLLQPELSMRLLRAKVSALEADAPQL
                     IATANIGCLAHLRSASSLRVCHWVELIAARLAGG"
     gene            12808..>13683
                     /locus_tag="VI195_10885"
     CDS             12808..>13683
                     /locus_tag="VI195_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6824937.1"
                     /translation="MADEWGERMPRSVSMLQTIAVSVGVAIGSGIFRVPATVAGQLQA
                     PGTILVCWVLGGVVALCGALSVAELAGALPRSGGIFAYLLEGYGPLPAFLFGWTELVV
                     IRAAALGAIATIFAEYLGYFMPLSAAQVHYTAALAIVAIGAINYVGVQRAAALLSATT
                     AVKYALLLALGLLAFTAIGGTAGHFRPAWPSALPLSLLATALIPVLWTYDGWADPASM
                     AGEISNPQHNLPRALIAGAACVMLLYLLVNVGFMYALAPAQMAGEKLIAASVAARIPL
                     LGGAGAALIAAAVVIA"
BASE COUNT         2214 a         4560 c         4471 g         2438 t
ORIGIN      
        1 tgtgcgcttg ctcccgcccg cggcgctcgt gcgcgcaggc ggactccttg tcgttgacat
       61 atcctcctgc ctgatcgcgc cgctccatcg tgcgcagcag ggacgtctgg agcacctatt
      121 ggtgcactgg cttttgtacc cagaattggt acatcccggc aaacgtatcg ggattatcgg
      181 cctcgaagtg cgcccagttg tgcagattgg tcgcagccgg atctcgagta aagcgcgtgc
      241 gataccggtg cagcacctgg gcatccagtt cgaagcccag gaaatgcagc ccggagtcgg
      301 agagaaacga ctcgatctgg tccaatgtca ggcaatgctc ctgtacgtgg aacagcaggt
      361 cacgacagtc actggtcgaa tagaagtcgg gtgcctggct cagccactgc agctcatcgc
      421 ccgcgcccaa tgcgcccaga tcgcgccgga cgcggcgaat gtcgtggagc gtaccctgat
      481 agcccctggc cgcaatgaat tcacgagctt tggccacgtg tctgcgggca atttggctat
      541 agaggccgat atgcatgacg ccgcccggcc gcaagcgcga aagcaggatt cgccatccca
      601 ggaaaggatc ggccagatga tgcagcactc caattgaagc gacgatgtcg aaggtgcgcg
      661 tgatgtcgcc gagcttcatg atgtctgcct gggcgtactc gatgttcgtg atgcccatct
      721 cgcgtgtctt gcgtttggca tagctgatgc tgctcaagct gaggtctact gccagcacgc
      781 gcacagagcg aattctgtgc gtagcaagaa ttggctgact gccggtgcca catcccgcaa
      841 tgagcatctc aggctggctg tcatcatgca ttggcacgaa acgcgcgagc ggaaacgtcc
      901 gtcgcagctc ggcattgaga cgccgctccg gccggacgct cggcagcttc acccagcgcg
      961 gatacggatt ctcttcatac tgctcgcgca ctttttctga cacaccgtcg tcgatcgcgg
     1021 tcaagcattc gatgccgcta cgcagtaccc gttcttcgag cggttcgcgc acctgttggc
     1081 gcaacagctc cgcaaccggc ccctgggcat ccgcagcaag cagccgcgca ggctcgggca
     1141 gtgtgcacag ggggaaataa gcggctacgg cgagcagcag caacggcggc ggcggcgcat
     1201 cggcgtcgag cagctccagt aacctcgagc ggcaagcctc tgccgcggcc cgctcgctct
     1261 cgtcacaatc aaaaatgtac tcattgatga agcattgtcg agcgagggcg gcatagaacg
     1321 gcagcgccgc gaggcctgaa ctatccggcg gtggctggtt tgttgcggcc tcgagcaatg
     1381 cgtggcgtgc ggcagtcagg aagcgctcga actcagtggt gcacacgggg gccgcttgca
     1441 acagtgcatg cagcagttga tcgcttgcca gagctgcgag gccctcgcga ccgaataaca
     1501 ccgtgcccgg tatgtgagcg ggccagcttt cgttggcgat gcgtacacag ctggcgatcc
     1561 ttgggtcgag catgagcaaa ctgattgccg gccggcttag ctggtaggga gtcccccacg
     1621 gttccgtgat cgcgaccgtg agcgccgcgc gtaccccggc gtcattggcc gtgaagcggg
     1681 tatacacggc acaataagcg aaggccgtct tgatctgccg gctcggtgca atctcgaggc
     1741 ggcgcgccgt cagctgcacc ccctccgggg acttgctgcg cgccatgaga atcagcgcca
     1801 ggttgtacag aatttcgtcc gactcgggct cgagggacaa cgctcgccga aagcttgcct
     1861 ccgctgcgtc gagctgcccg aggctgctgg cggtctggcc gagcgcgtga tgggccgccg
     1921 cgtagtcagg ccggatatcg atcgcccggc gcaggctgtc tgcggcctgt tcatgccgcc
     1981 cggcggccat cgacagcaga gccagattga gatggacacc ggcatccccc ggatcgatcg
     2041 ccagcgcgcg ctcgtaactc gctatggctt cggaggtacg tccgaggtcc tcgagtgcat
     2101 tcccctgagc caccagcgca gccgcaaaat cgggcttgat gccgagggca gcccggtagt
     2161 tgtccaaggc gtgctgacgt tcgccggtac gatagttgag gtttcccaaa ttgaagtggg
     2221 cggccgcata gtctggatcg ttggctatgg ccagccggta cgcagcgcga gcctcatcga
     2281 cctgctcgcg cgcaagcaga atgttgccgc gattgaggtg tgcccgcgca cagtgcgggt
     2341 ccgcctgggc ggcggcatcg taacgcgcca tggcctcggc ggtccgtccc tgttcctcca
     2401 gtgcgttccc ttcatcgatc agcgcgactg cggagtctcg cgagggggcc tgaagagctt
     2461 cgtcaatcgc ggcagccgtt ggcttcatgc gactcctctc gagatcctca ggtgcgggtt
     2521 ggctcgctca ttgacgcgta acatcccggg cgtctcagcc atcttgcccg atcagcaggt
     2581 tgcggagcgg cgtgcgcgtt cacaattgaa ggagccagac ggcgccatct caaacccgca
     2641 gcgctgtgcg cgaatgctca ggcgagcgac acccgcagct tcaaaacagg ggtcgcgctg
     2701 cggcgacagt tcagcgagcg accactccct gcttcgccac ctggcttgcg acagcgtcgg
     2761 caacctcagt gatcaggctt tgcactccct cagggttgct cgttcggctg gtacccgccc
     2821 agagaagctt atcctgggtg agtgagtaga tcagagtttc aatcgtcagc acttcgtcag
     2881 tctgaaccga cccggtggaa taggtgagtt ccatcccata cgaggaatag taagagccga
     2941 atgttccgta cgcgctgccc gagaagccgc ccgggtcgac gatggtgtgc gtgtcatggc
     3001 caaccaggcg catgatcacg actccttcga cacccgcttc cttgaaacgg gtgcgcgcgt
     3061 aatccacggt ggggtgatcg aggcctatca gcgtgtatgc agcgacaccg cgtgcaccgc
     3121 ggatggtgag ctcgtgagtt agatagacct ccgcatcgcg ccgccgcttc ttgtcgctca
     3181 cgatgaccag tgccgcgatg ttcttgccta ccggatcgac gcgctgcgcg tccggcgcct
     3241 tccaggtcga ggtaaacgtc gtgctgccgc aggcgcccag gcacagagct gcaccacaaa
     3301 tcaaagcgcg caacgtttgt ggcaagtgtt cgtcccatcc cccgagttaa cggacactag
     3361 cgtgagtgtc ctgcccgctg caagcggtcg ctcggcgacg ccgcgcgcgc tcgcgcatta
     3421 ccaggcctcg accgcctcga ccagcacgac ctcgcaggag cccggcccag agtcgctgcg
     3481 cgtcaatgaa gtattgagat acgcaccatc ctggcgcacg ccgttgacca gatagccggt
     3541 gagatgaact acctgaccca cgcgcaggcg ctgcagtttg cctgccacca cccgatcggc
     3601 gggaatgacg tgcgtattcg cgctgtgttc gatgacggcc tgtcgcggga tcggtagccc
     3661 gcgcttcggc ttccaggaat agaatcttgc cccctgcgtg atctcaaaag cctcgagcac
     3721 ccgattgtcg gacatggctc cccagccgag tgcgagatct tcgggtatga gatcggccag
     3781 ctggtcgaag cggtagtcct cccgaccgag aatgcgcgcg gtgatctcgt aatgcgcgcg
     3841 cgctgtgagc cgccactgac ccagcaccgt tacccgtggc gcctcgatat ctgtctgcct
     3901 tgggtcatcc ggggcgatcg caccgtctgc gggatgcacg gggcgcagtc gccagttttc
     3961 atacccctgc cataaccctg caactgccaa ggcaatgaga agaatgcggc gcaatggcat
     4021 gcctgggtat tgttccagca cggcgcgcca caccgccgtg gcacaggtcg agggccgaac
     4081 ggcgcgaagc gtgagtcgcg tcgcacgctc gagcagcgcg ggtcagtttc ttaccagcac
     4141 tggcgtcgcg ggattcacct gagctgcagc accggccggg cggtgcaggt gaaattgagg
     4201 gtgctggtct gctcgccctt ggggctcact ccgggcttgt cttcgaagct ttcgatcgcc
     4261 gccgtttgcc gggactttcc gcagaactcg ttcgcttgcg caattgtctc ctcgcggcta
     4321 ccggtataac caccccagtt ggcgcttgca gtcacggcat gcttgccgtt gccgaggtcc
     4381 tggacatggc tggtgtgagc gcaaccgcca agcagcagcg cgctcatgag gatggccatt
     4441 ttcatcccgc tcacctcctt gcgtgtccgc agcgacgata caccacggct gccttattgg
     4501 actcgaagtc ttggtatcgg cctgtgccgc cacgccgccg tgttcacttg ttctgcggcg
     4561 ccttgccgat cgatgcgggc cgcattgtgg ccccgagcac ccggggctgt catccgcgaa
     4621 cgcaggcgag accaatggat cccgattgcg tgcggtatgc acggccggac cgcaggctgc
     4681 gtgtgaacgg gctcacggtt ggtgagtgaa ccgcaccgca gaatgcctgc ctggcagatc
     4741 tgcacgcata cggtaccggt catgacaatg atgcaactga tcatcggcgc cgcgctcggc
     4801 ttcgtcgtgg cccagagcgg gctgtatggc atcagacact ccctgggctg gctgcagcgc
     4861 gaggaggtca gcacgcgcgt gcggacgctg cccgggcatg tcatcgtcgg cgggttcatc
     4921 cgctacgcgg ctccgatcgg cgcaactgcc gctctcatca ctctcgctgt ctggggggtg
     4981 ggcgactacc ttgccgccag gaccgcgcgc gacgccacac tcgccaacgc gctcgattcc
     5041 gccgcctctg ccggcacggc gcaggtgcag ggctcggcgg atgagtccag tgtggccagt
     5101 gcgtccgccc cgtccgagtc ggccagctcg atccagtccg agggcgtcga cccttacgcc
     5161 gatccggact acaaggtaca tcggcgcgtc caccggacgg gcagcccggt gactctcaag
     5221 gagacgctgc tgcaacgctc ggaagccaag gcccgctccg agctgctgca ggacatgcag
     5281 caacacgcgg cccgctcgca atacgactgc gaggccgccg accgcgcagc gcgctacctc
     5341 aaggcgggcc tggatgtgtg gggttttggc gcctggcagc tcaagtattt cccggtggag
     5401 ggctacaaag gcgcgacgct cggcgggtgc aaggacattc cgaacgtcgt cgatccctcg
     5461 cagctcaacc tgcaatccac cgttgcccag gagaaccact cctgagcgcg actcggtggc
     5521 gcgcgtctgc tacgccgcgc tagtaaatca atgggatcct cgcggcgagc gaggcgtcca
     5581 gcgccgcgtc gagcttctga tcgatctgga agaagcgcgc ggtcgtgcgg gccgaaagct
     5641 ccttgttgaa cttcttcgca tagtcttcct tgagctcggt ccgctccttg tcgatgtcga
     5701 gagcttcctt gaggagccgg tgcccctcgt cctcgctcat ggaggtgcgg ttggccgcgt
     5761 agtcggtgat gagcttcacg cgccggtcgc cgagcttgct catgtccgca cgatattgat
     5821 cgtagatcgg ccagaagcgc gcggcttcgc tctcagtgag cagcatgttc gccttcacga
     5881 tcgcgcggcg gtccttgccg acttcctgcc gcagcatctg gatggcgggc tccagatcgc
     5941 tgaacttttc atttacgacc ggcttgtctg cggcgagcgc gaatgctgga gcgagcagcg
     6001 cgcagagcgc gccagtgcga aggtacggaa gaattctcac gtcggctctc cttggaccag
     6061 ggcgaacaga acggccacac cgtcaactcg tgagttcttt ttttacctcc gcgccacgag
     6121 cgcgtacata ggacccttgg cgtaccgctg cgatgtgcat cggcggcgcc gcttcgcata
     6181 acttattgtt ttggtttatt ttctcgaaga gcccgtgcgt ttcaaacggg ctgctcgatg
     6241 gagcgggcga ggggcgtgaa cagcttcggg ccggcctcgc tcatgtacca acagtcctcg
     6301 aggcggatgc cgaactctcc ggggatgtac agtcccggct catcggagaa gcacattccc
     6361 ggctgcagtg cagtcttgtc gctgcgcacg aggtagggcg gctcgtgcac atccatgccg
     6421 atcccatggc ccgtgcggtg cgacagtcca ggcagcgcgt aatccgggcg ccaacctttc
     6481 tgttcgtaga aggcgcgcac cgcccggtcc accgcccccg ccgcgactcc gggcttcgcg
     6541 gtctccaggg tgagttcctg gccccgcttc actgtgtccc acagttcgcg ctgacgcgcc
     6601 gagatctcgc cgtagaccca gctgcgggaa atgtcggact ggtagccgtg caccgagcag
     6661 ccggtgtcga tgagaatgac cgagccctcg cgcacctgct gcggcttgat cgagccatgt
     6721 gggtaggcgc ttgcctcatt gagcagtacc agtgtgaact catgcgagcc gccgagctgc
     6781 tcggtagccg ccaccatcag agcgaggatg tccgtcgctt tcatgccggc acggacacgt
     6841 cggtgcgtgt agcgtagagc ggcaagtgtg acgctgttgg ccgcctgcag aagcgcgagc
     6901 tcggagggcg acttcaccat tcgacaggcg cgcaccaagt catcccccgg aatgacgctc
     6961 ggcttcgtcg ccccgccagc cgccacgacc tgttccacga tgaaatagcg cgtcgtcggc
     7021 tcgatggcca gcgcaccccc gcggccgtga tcgcgccacg cgccggcgag caacgcgaag
     7081 gggctctcgt cctccttcca tgggcgcaca tcggcgggta cccgcagggt ttcccggatc
     7141 gagggttcct cgaaggccgg cgtgacgacg atgaccggtc cctccgctgc cacgagcgcc
     7201 gcagtcgtgc gctccgagcg ccaccagtga atgccggtga agtactcgag tgtggacccg
     7261 gactcgatca gtattgcgcc ggtcctgcgc tgctgcatca gcccctgcag cttcgcaagc
     7321 cgggcaccat gctgcgcggc atcgatcggc acgacgttgc gcgtgagcga gctcagtccg
     7381 ctcaccggcg cgcccgcgtg aacgggggcg ccggcggggc ctgcgatcgc agctccggcc
     7441 gcagacagtg caagaaactc tcgtctggag actctcatgg ttgattcccc tgtggctggc
     7501 gcgccatccc ctgcggccga atgcgcggac ccggccagcc ccgactgctt ttgtgaccgg
     7561 cgtcactcgg cgccggccgg ggcccgccgc agggtgcgat tttgagaggc caggacagga
     7621 agataaagag cgttcgacgt agttttatgt agtgaatatc cgtctcaagg tcatcgcact
     7681 gatcggtgcg atcttcgcga ttctcgtcgg ggccgaagtg ctggtcgagc ggcacgtggt
     7741 catgccgagt ttcacgcagc tcgagcgcgc ggactcccag accgcgatgc ggcggatcgt
     7801 ctacgccctc gaccgtaccc tcacggagct cgagctgctg gcgaccgact ggggcaactg
     7861 gggcgatacc tatcagttcg tcgaggacca caacccggcc ttcgtcgccg ccaacatcag
     7921 caacatcgcc ctgcggcagc tgaatgtgaa cgtgttgatg atcgtcgatc gcgacggcaa
     7981 gctggtcgag gcgcacgacc tcgacctgca gtccgaccag ccgctcggac tcgagctcac
     8041 ctcacagccg gcgctgccgg cggactttcc gtggcgggct aacctgcgca ccggaacagc
     8101 gacccgctgc ctgttgcgga ccagccgcgg catcctgctg ctcgcggccg cagcggtgct
     8161 cgatggccac ggcaacggcc ccgcgcgtgg catggtcgtc atcggacgtc tgctgtcacc
     8221 cgcggtggtc cgcagcctcg gtgatcaggc gcaggtgcag ctcgccatgc tgccgccgga
     8281 tccgaacacg acgagcgagc ggatgctcga gagcgacagc ctcatccagg tgtaccgcgc
     8341 cttcaacgac ctgtacggca agccagtcat gacgttacgg gtcgatgtgc cgcgggagat
     8401 ctcccggcgc ggttaccgcg cagtcacgta cgcctccgcc tgcctgatcg cggcggcggt
     8461 gatcgtgctg ctgctgctcg tgctggtgct caatcgggtg atcctgaatc cgctcgcgca
     8521 agtgacgcgc catgccgtgg cgctcggtgc ggacaaggat ctcaccacgc gactcgatct
     8581 gaagcgcggc gacgagttcg gggtgctcgc gcgcgagttc gatcgtatgg tggagcgtgt
     8641 cgaggagtcg cgtatgcagc tcgtcgacca gtccttccag gcgggttttg cggagctcgc
     8701 caagggggtg ctgcacaacc tgggcaatgc catgacgccg atctccgtgc gtctcgccag
     8761 cctcaccgac cgcctgcgcg ccgcgcccac cgcggacgcc gaacaggcct tcgccgaact
     8821 gaagcaggga ggcgccgacg cacagcgccg cgccgacctc gaaaaattcc tgcagctcgc
     8881 ctgccgggag ctcgccgcca cgctgcgctc gtcacaggaa gatctcgcca ccatcacgcg
     8941 ccagacgggt ctcatccagg cggcgctcgc ggagcagatg cgctcggcgc gcaacgaggc
     9001 cgtgatcgag ccggtgcgcc tcaccgaact cctcgcgcaa tctctcgaca tcgttcccga
     9061 cagctgccgc gcccggctca gcatcgatgc cgacgatacc gtgcgccgtg tcggggtggt
     9121 gcaggtcgcg cgcacggtgc tgcggctggt cctgcagaac ctcatcatca atgcggcaga
     9181 cgcggtgcgc gatgccggca aggacaaagg caagctgcgc gtgagcgccg ccatcgtgca
     9241 cgaggccgat ggccggcagc tgcacctgca gtgcgtcgac gacggcgtcg gcatccccgc
     9301 cgagaatctc gagcgcgtgt tcgagaacgg ctttaccacc aagtccccgg aaaccaacca
     9361 cgggatcggc ctgcactggt gcgccaatgc catcggcgcg ctcggcggtc gtatctgggc
     9421 cgcgagcgac ggtccgggaa gtggcgccgc actgcacctg ctggtgccac ttggcgcccg
     9481 tgaaactgct cccgcggcag gagccgcctg aggtcctcat ggccgaacgc aacaagccca
     9541 ttcgagtact ggtcgtcgac gacgagccgg aaatccgcga cgcctacaag cagatcctgt
     9601 gcgagaccga ggtcaacctc gagatgaccg gttttcacga gctgcgcagc cgcctgttcc
     9661 gtaagaatcc gatggaagcg ctgcgccagg cggccacgcg atcgacgggc ttcgacacgg
     9721 tgttctgcca gcaggcgagc gaagcggtgg cggcggtcaa ggaggcgctc gcgcgcaacg
     9781 agcccttcgc ggtggccttc atcgacatgc gcatgcctcc cggggcggat ggggtgtggg
     9841 cggcgacgca gatccgcgag ctcgatcccg cgctcgaaat cgtgatctgt acagcctact
     9901 ccgacgccga tcccggagag atcggcggct acgtgccgcc ggaggacaag ctctcctacc
     9961 tgcagaagcc gttccatccg cacgaagtgc ggcagatgac catcgcgctc ggcagcaagt
    10021 ggcgcgccga gcggcgcatc gtgcggctgg cgtacttcga tacgctcacc gggcttccca
    10081 accgcgagca gtcgcgcaac cgcctgatcg gcgctttgca ggcggccaag gacaagaacg
    10141 gtctgctggc ggtactgtat ctcgacctcg ataacttcaa acgggtcaac gacacccttg
    10201 gccacgcagt cggcgatcag ttgctgtgcg tcgtcgcgga ccggctacgc agctcgctgc
    10261 gctacggcgc cgactcgaat tcgggcggcg gcggcaaccg tctgggcgac atcgcccggc
    10321 tcggcggcga tgagttcatg gtgctcctgc ccaacttgcg taacacggtc gatgccggcg
    10381 gagtcgccga gcggctgatc ctctcgctcc gccagccgat ccagcttgcc tccaactcgc
    10441 tggtcgtcac tccgagcgtc ggcatcgcgc tctatccaca ggacggcacg gacgccgaga
    10501 cactgctgcg taacgccgac cttgcgatgt acttcgccaa acggcgcacg cccggcacct
    10561 tcgccttttt cgatgtttcc atgaacgctt ccgcactgca ccgcttcacg atcgaggagc
    10621 agctgcgcgg cgccctggag cgcaacgagt tctcgctcca gtaccagccg cagttcgatg
    10681 tgcgcagcgg cacgatctcc ggggtggagg cgctgctgcg ctggaccaac gccgagctcg
    10741 gcgcggtgag tccgctcgag ttcattccgg tggccgagga gaccggcctc atcctcgcga
    10801 tcggcaagtg ggcgctgcgt gcggcctgtc agcaggcgca ggcctggcgc gccgaggggc
    10861 tgccggtgca gcgtatggcg gtgaacgtct cgggtcggca gttcgcgctc gccgagtatc
    10921 cgcaggaggt ggccgagatc ctcagcgaga ccggactcga gccggcgatg ctcgagctcg
    10981 agatcaccga gtcggtcgtc atggcgaacg aggcctgggc cgagaaggcg atcaaggagc
    11041 tcaaggcgct cggcatatcc ctggcgatcg atgatttcgg caccggttac tcgagtttcg
    11101 gtcgcctgcg gcacttcgag gtcgatcgtc tgaagatcga ccggtcgttc gtcaccagca
    11161 tcagcgatca cagcgatgat cgtgccatcg ccgcggcgat catcgccatg tcgcgctcgc
    11221 tgcacatcca cgtcaccgcc gaaggcgtgg agaactaccc gcagctcgcc ttcctgcagg
    11281 agcaggagtg ccaggatgcg cagggtttcc tgctgagccg gccgctgcag gccgatgcgg
    11341 cacgcgagct gctgcggcgc gtgggcgagc tcgctgatgc ctcgcgcacg cagcgcctga
    11401 agatcatcac gggctaaaaa gtaccccgcg gaatttcgcg gccctcacgc ggagcaccgt
    11461 cccctgcagg attgcaggta gaattgcgcc ttcacgactc ccgcccgtcc gcgggctgat
    11521 ggaatcggga ggttatgcag accgcgctcg cggatttcat cagcaactcg cccgcaggcc
    11581 gccaggcgga tgccattctg cgcagctgcg tgcactgcgg cttttgcctg cccgcctgtc
    11641 cgacttatca gctgctcggc gatgagctcg acagtccgcg cggccgcatc tacctgatga
    11701 agcagctgct cgagggtgag cccgtgagcg cgcctacgca gctgcacctc gaccgctgtc
    11761 tcacctgtcg cgcctgcgag agcgcctgcc cctcgggcgt gcgctacgga cggctgctcg
    11821 atatcgggcg cgctgtggtg gaggagcggg tgccgcgccc gctgcccgcg cgcgggcggc
    11881 gctacctgct gcgcaagatc gtgccctaca cgcgccgcgt acgtgcactg ctcggcctcg
    11941 cagcggccgt gcggccgttg ctgccgcgcg cgctgcgcgc tgccgtgcca aacgccgagg
    12001 cgcgcgcctc accgctcgag caactggagc gaccgtcgct ctggccgcct gcgcgccatg
    12061 cgcggcgcgt cgtgctgctc gagggctgcg tgcagccggc cctcgccccg ggcattaatc
    12121 cggcggcggc gcaggtgctc gaccgcatcg ggctcagcac gatccgtgtt gcggccgcgg
    12181 ggtgttgcgg agcgctgtcc caccatctcg ccgcgcatca ggaatcgcgc gcgcaggcgc
    12241 ggcgcaacgt cgatgcgctc tggccgcacg tgcaggctgg cgcagaggcc gtagtgttcg
    12301 ccgccagcgc ctgcgcagcg atggtgtgtg actacggaga gctgctgagc gatgacccca
    12361 gctacgccgc gcgcgccgcg cgggtgagcg cgctcgcgcg cgacattggc ccgctgctcg
    12421 ccgctcatgc cggggcgctg cgcgccgcgc ttgctgcggc gagcagcgcc gcgcctgtgc
    12481 cagccgctgc gcgcgtggcc tttcatgcgc cgtgcactct gcagcacgcc ctgcgcgcct
    12541 cgggcgtggt cgagccgctg ctgcaggcgg cgggcttcac cctcaccgca gtcgccgatg
    12601 gcgcgcgctg ctgcggttcg gcgggtactt actccctgct gcagccggag ttgtccatgc
    12661 ggctgctgcg cgccaaggtg agcgcgctcg aggcggatgc gccgcagctc atcgcgacgg
    12721 ccaacatcgg ctgtctcgcg catctgcgca gtgccagctc gctccgtgtc tgtcattggg
    12781 tcgagctcat cgccgcgaga cttgccggtg gctgatgagt ggggcgagcg catgccgcgc
    12841 tcggtgagca tgctgcagac catcgcggtc tccgtggggg tggcgatcgg cagcggaatc
    12901 ttccgcgtgc cggcgacggt cgccgggcag ctgcaggcgc cgggcacgat cctcgtgtgc
    12961 tgggtactcg ggggcgtcgt tgcgttgtgc ggcgcgctgt cggtcgccga gctcgccggt
    13021 gcgctgccgc gttccggcgg catcttcgcg tacctgctcg agggctacgg tccgctgccc
    13081 gcgttcctgt tcggctggac ggagctcgtg gtgatccgtg ccgcggcgct cggggcaatc
    13141 gccaccatct tcgccgagta cctcggctac ttcatgccgc tctcggcggc gcaggtgcac
    13201 tacacggccg cactcgcgat cgtagccata ggcgcgatca actacgtcgg cgtgcagcgc
    13261 gccgccgcgc tgctgagtgc aacgaccgcg gtgaaatacg ccttgctcct ggcgctgggt
    13321 ctgctggcgt tcaccgcgat cggcggcacc gcgggccact ttcggcccgc gtggccgagc
    13381 gcgctaccgc tctcgctgct cgccaccgcg ctcatcccgg tgctctggac ctacgatggc
    13441 tgggccgatc ccgcctcgat ggccggcgag atcagcaatc cgcagcacaa tctgccgcgc
    13501 gcgctcatcg ccggcgcggc atgcgtcatg ctgctctacc tcctggtcaa cgtcggcttc
    13561 atgtatgcgc tcgcacctgc gcagatggcg ggcgaaaagc tcatcgcggc cagcgtggca
    13621 gcgcgcattc cgctgctcgg tggcgccggc gcggcgctga tcgcagcggc cgtggtcatt
    13681 gca
//
LOCUS       DATDMP010000618         3281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1652987, whole genome shotgun sequence.
ACCESSION   DATDMP010000618 DATDMP010000000
VERSION     DATDMP010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652987"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /gene="map"
                     /locus_tag="VI195_10890"
     CDS             <1..711
                     /gene="map"
                     /locus_tag="VI195_10890"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY6824938.1"
                     /translation="HVKAGVTTGKLNDLCHEYMVTVQRTVPAPLHYAPPGYRPFPKSI
                     CTSVNHQVCHGVPGERVLKPGDILNIDITVIKDGFHGDCSRMFYIGEPSIQAKRLVEV
                     TFECMWRGIFEVRPGARLGDIGSAIQQHAEENGFSVVREFCGHGIGRKFHEEPQILHY
                     GKAATGMPLEPGMTFTIEPMINAGKPGIRELADGWTIVTKDHSLSAQWEHTVLVTESG
                     YEVLTNSPGVLPPPARGA"
     gene            714..>3281
                     /locus_tag="VI195_10895"
     CDS             714..>3281
                     /locus_tag="VI195_10895"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY6824939.1"
                     /translation="MDADFREELAQARSRLRASYLERPNPGLLLRRHARVIDRIVKAV
                     WAQAGVRGAALVATGGYGRGELFPHSDIDLLILLAVPPAAEQNAALERLIGKLWDIGL
                     EIGHSVRTVDECVEAAGQDITINTSLRESRYLTGSRRLFAALIEAMAARSDVPSFLQA
                     KKLEQEQRHAKHQDSPYALEPNVKESPGGLRDLQVIQWIARASGIGKRWKDFVPAGLL
                     ERDEALQIARHEGILQDLRIRLHYLAGRREDRLVFDFQAALAAQLGYAATADRRASEV
                     LMQRYYRSAKVITQLNTILLQNLEARIAPQPLQAPRLLNERFRARGELLEAMEEALFE
                     REPSAILESFLLMMQHPDLRGMTAPTLRALWRARTRINARFRADPLARLLFLHILQQP
                     RGIVHEFRRMNQYGVLSRYLPEFGRIVGQMQHDLFHVYTVDQHILMVIRNLRRFTMPE
                     LAHEFPLCTELMAGFERRWLLYIGALFHDIAKGRGGDHSHLGSADAQRFCKRHGLSAE
                     DTGLVCFLVEQHLSMSSVAQKKDVHDPEVVSAFAAQVGTERRLVALYLLTVADVRGTS
                     PKVWNAWKGKLLEDLYRATRRVLTGEPLARDAALAEKQGEAMRLLRLYALSDSVKDKL
                     WAQLDITYFLRHDPQEIAWQTRMLHYRVDTAQPVVKARLAPFGEGLQVMIYTRDREGL
                     FARICGYFERAGFNIVEAKVHTTRNGYALDTFLVLGERSDGHYRDMLSLIESGLATEL
                     ESEAPLQPGHSGRLSRRVRHFPISPVVDIRPDERGTYHVLSIVASDRPGLLYGVARLL
                     ADYHLNVQTARINTLGDRAEDVFLVSGQAVSQPKTVLQLERDLMKELSLQAPAPVLET
                     "
BASE COUNT          564 a         1122 c         1064 g          531 t
ORIGIN      
        1 cacgtcaagg ccggcgtcac cacgggaaag ctgaacgacc tgtgccatga gtacatggtc
       61 acggtccagc gtacggtgcc cgcccccctg cactacgcgc cgcccggcta ccggcccttc
      121 cccaagtcga tctgcacctc cgtcaaccac caggtatgcc atggggtgcc gggcgagcgc
      181 gtgctcaagc ccggcgacat cctcaacatc gacatcaccg tgatcaagga cggcttccac
      241 ggggattgca gccgcatgtt ctacatcggc gagcccagca tccaggcgaa gcgcctggtg
      301 gaggtgacct tcgaatgcat gtggcgggga atcttcgagg tccggcccgg cgcgcggctg
      361 ggggacatcg gttccgcgat ccagcaacac gccgaggaga acggcttctc cgtagtccgg
      421 gagttctgcg ggcacggcat cggccgtaaa ttccacgagg agccgcagat cctgcattac
      481 ggcaaggcgg ccacgggaat gccgctcgaa ccgggcatga ctttcaccat cgagccgatg
      541 atcaatgccg gcaagcccgg tattcgcgag ctcgcggatg gatggaccat cgttaccaag
      601 gaccacagcc tctcggcgca gtgggagcac accgtgctcg tcacggaatc cggttacgag
      661 gtcctcacca attccccggg cgtgctgccg ccgccggcgc gcggcgcctg accgtggacg
      721 ccgattttcg cgaagagctc gcgcaggcgc ggagcaggct gcgcgcgtcc tatctcgagc
      781 ggccgaatcc ggggctgctc ctgcgccgcc acgccagggt gatcgaccgg atcgtgaagg
      841 ccgtctgggc gcaagcgggg gttcgcggcg ccgcgctggt ggccaccggc ggctatggcc
      901 gcggcgagct ttttccgcat tccgacatcg acctcctcat cctgcttgcc gtcccacccg
      961 cggccgagca gaacgcggca ctcgagcgat tgatcggaaa gctgtgggat atcgggctcg
     1021 agatcggaca cagcgtgcgc accgtggacg agtgcgtcga agcggccggc caggacatca
     1081 ccatcaacac cagcttgcgc gaatcccgct acctgaccgg aagccgccgg ctcttcgccg
     1141 ccttgatcga ggcgatggcg gcccgctccg acgtaccgtc cttcctccag gcgaagaagc
     1201 tcgagcagga gcagcggcac gcgaagcacc aggattcgcc ctacgccctg gagccgaacg
     1261 tgaaggaatc gccgggcggc ctgcgcgacc tgcaggtcat ccagtggatc gcccgcgcga
     1321 gcggcatcgg aaagcggtgg aaggacttcg tgccggccgg gcttctcgag cgcgatgagg
     1381 cgctgcagat cgcgcggcac gaaggcatcc tgcaggactt gcgcatccgg ctgcactatc
     1441 tcgccggccg gcgggaggac cgcctggtct tcgacttcca ggccgcgctc gcagcccagc
     1501 tcgggtatgc cgcgacggcg gaccggcggg cgagcgaagt tctcatgcag cgctactacc
     1561 gctcggcgaa ggtcatcacg cagctgaaca ccattctgct ccagaacctg gaggcgcgca
     1621 ttgcgccgca gccgctccag gctccccggc ttttgaacga gcgttttcgc gcgaggggcg
     1681 agctgctcga agccatggag gaggcgcttt tcgaacgcga gcccagcgcc atcctcgaga
     1741 gcttcctgct gatgatgcag caccccgacc tgcgcggcat gacggccccg accctgcgag
     1801 cgctttggcg cgcgcgcacg cgcatcaacg cgcgcttccg cgccgatccg ctggcgcggc
     1861 tgctgttcct ccatatcctg cagcagccgc gcgggatcgt gcacgagttt cgtcgcatga
     1921 accagtatgg cgtgctgagc cgctaccttc ccgagttcgg ccgcatcgta gggcagatgc
     1981 agcacgatct tttccacgtc tacaccgtgg accagcacat cctcatggtg attcgcaacc
     2041 tgcggcgctt caccatgccg gagcttgccc acgaatttcc gctttgcacc gagctcatgg
     2101 ccggattcga gcggcgctgg ctgctttaca tcggcgccct gttccacgac atcgccaagg
     2161 gccgcggcgg cgaccactcg cacctgggtt cggcggacgc ccagcgcttc tgcaagaggc
     2221 acggcctatc ggccgaggac accggccttg tgtgcttcct cgtcgagcag catctttcca
     2281 tgtcgagcgt ggcgcaaaag aaggacgtgc acgacccgga agtcgtgagc gccttcgccg
     2341 cccaggtcgg gaccgagcgg cggctcgtgg cgctctacct cctgaccgtg gcggacgtgc
     2401 gcggcacgag ccccaaggtg tggaacgcct ggaaaggcaa gcttctcgag gatctttacc
     2461 gcgcgacgcg gcgggtgctg accggcgagc cgttggcacg cgacgcggcg ctggcggaaa
     2521 agcagggcga agccatgcgg ctgctgcgcc tctacgccct ctcggacagc gtgaaggaca
     2581 agctctgggc gcagctcgac atcacgtact tcctccgcca cgatccgcag gaaatcgcgt
     2641 ggcagacgcg aatgctgcac taccgcgtcg ataccgcgca gccggtcgtg aaggcacggc
     2701 tcgcgccttt cggcgaaggc ctgcaggtga tgatctatac gcgggaccgg gaaggcctct
     2761 tcgcccgcat ctgcggctat ttcgagcgcg ccggattcaa catcgtcgag gcgaaagtcc
     2821 acaccacgcg caacggctat gcgctcgaca cgttcttagt gctcggcgag cgctccgacg
     2881 gtcattaccg cgacatgctg agcctgatcg agagcgggct ggccaccgag ctcgagtccg
     2941 aggctccgct gcagcccggc cacagcggcc gcctttcgcg gcgagtgcgc catttcccga
     3001 tctccccggt ggtggacatc cggccggacg agcgtggcac ctatcacgtg ctctcgatcg
     3061 tggcgagcga ccgccccggc ctgctctatg gcgtggcgcg actgctcgcg gattaccacc
     3121 tcaacgtgca gaccgcgcga atcaacacgc tcggcgatcg cgcggaggac gtgttcctgg
     3181 tgtcaggaca ggcggtgagc cagccgaaga cggtgcttca gctcgagcgc gatctcatga
     3241 aggagctcag tcttcaggcc ccagcgcctg tcctggaaac a
//
LOCUS       DATDMP010000619         3233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2098394, whole genome shotgun sequence.
ACCESSION   DATDMP010000619 DATDMP010000000
VERSION     DATDMP010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098394"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VI195_10900"
     CDS             complement(<1..1171)
                     /locus_tag="VI195_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY6824940.1"
                     /translation="MSALHARTLTQLADGLRRKSFSSVELVGELLTRIERSQPELNAF
                     ISVTGAAALEAARAADRALATGSAGALAGVPIAHKDIFCTAGVRTTCGSRMLDNFVSP
                     YDATVVAKLKRAGAVVLGKTNMDEFAMGSSNETSFYGPVRNPWNRQVVPGGSSGGSAA
                     AVAARLVAGATGTDTGGSIRQPAALCGITGMKPTYGRVSRYGMVAFASSLDQGGTLTA
                     SAADAALMLREMAGFDPRDSTSVATPVPDYVAALEQPIAGLKVGLLAEFFDKGLDADN
                     ERCVREALRVLESLGARVTQVSLPSLPLSVPVYYVVAPAECSSNLARYDGVRFGHRCE
                     SPRDLNDLYRRSRGEGFGAEVKRRIMTGTYVLSAGYYDAYYLKAQRVRALINADFI"
     gene            complement(1179..1469)
                     /gene="gatC"
                     /locus_tag="VI195_10905"
     CDS             complement(1179..1469)
                     /gene="gatC"
                     /locus_tag="VI195_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY6824941.1"
                     /translation="MALTREQIATIATLARLELSAEEVPVYQESLSRILEFVGALERA
                     DTTDVAPMAHPLAGLTQRLRPDEVTEGDAHERYQLNAPSTAAALYLVPKVIE"
     gene            1600..2643
                     /locus_tag="VI195_10910"
     CDS             1600..2643
                     /locus_tag="VI195_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY6824942.1"
                     /translation="MLKRLRGYFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVAVQDE
                     PTRGGKVIVAVGSEAKAMLGRTPGHITAVRPMKDGVIADFTYTEKMLQFFINKVHGNR
                     TLKPSPRVLVCVPFGSTQVERRAIRESAEGAGARNVAIVSEPMAAAVGAGLPVHEARG
                     SMVLDVGGGTSEVAVLSLNGVVYANSARIGGDRFDEAIMNYVRRNYGILIGEATSERI
                     KIEIGSAYPGQQVRELSVKGRNLSEGVPRSFTLNSNEILEALQEPLQAIVSAVKQALE
                     QTPPELGADVAERGIVLTGGGALLRDIDKLLTEETGLPVVVADDPLTCVARGGGRILE
                     LMDELGPGHFGLE"
     gene            2672..>3233
                     /gene="mreC"
                     /locus_tag="VI195_10915"
     CDS             2672..>3233
                     /gene="mreC"
                     /locus_tag="VI195_10915"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY6824943.1"
                     /translation="MAAFGTGAGRPLSGRGGSPGFRFTLYALLSVIVMYLDQRAHYLE
                     QVRYVLQATAYPLQLAVNSPTAAWRWLQDTFRSREALQAENTRLRANARDLELRAMRY
                     EALARENGELRGLREALPPVADRWLPAEIVNIQLSSLRQRLLLNRGAANGVFKGQAVL
                     DDRGLIGQTTHVGPWSAEVILITDPEH"
BASE COUNT          527 a         1091 c         1096 g          519 t
ORIGIN      
        1 ggatgaaatc cgcgttgatg agcgcacgca cgcgttgcgc cttcaggtag taggcgtcgt
       61 agtaaccggc ggacagcacg taggtaccgg tcatgatgcg ccgcttgacc tcggcgccga
      121 agccctcgcc ccgcgagcgg cggtacagat cgttcaggtc gcgcgggctc tcgcagcggt
      181 gcccgaagcg caccccatcg taacgcgcca gattcgagga acactccgcc ggcgccacca
      241 cgtagtaaac cggcaccgac agcgggaggc tcggcaggct cacctgcgtc accctcgcgc
      301 cgaggctctc gagcacccgc agggcttcgc gcacgcagcg ctcgttgtcg gcgtccaggc
      361 ccttgtcgaa gaattccgcc agcagcccca ccttgagtcc ggcgatcggc tgctcgagtg
      421 ccgccacgta gtccgggacc ggcgtagcga cactggtcga gtcgcgcgga tcgaagcctg
      481 ccatttcgcg cagcatgagt gcggcgtccg cggcgctcgc ggtgagtgtg ccgccctgat
      541 cgagactcga ggcgaaggcg accatgccgt agcgcgatac ccgtccgtag gtgggcttca
      601 tcccggtgat gccgcacagc gccgccggct ggcggatcga gcccccggta tcggtgccgg
      661 tcgcgcccgc gaccagccgc gcggccaccg ccgccgccga gccccccgag gagccgccgg
      721 gcacgacctg ccggttccag ggattgcgca ccgggccgta gaaactggtc tcgttggatg
      781 agcccatggc gaactcatcc atgttggtct tgccgagcac caccgcgccc gcgcgcttga
      841 gcttcgcgac cacggtcgca tcgtagggcg agacgaagtt atcgagcatg cgcgagccgc
      901 aggtggtacg cacgcccgcg gtgcagaaga tgtccttgtg agcgatggga acaccggcga
      961 gcgcacccgc gctgccggtg gcgagcgcgc gatcggcggc acgcgccgcc tcgagcgccg
     1021 cggcgccggt cacggatatg aacgcgttga gctccggctg gctgcgctcg atgcgcgtca
     1081 gcagctcacc caccagctcc acgctcgaga agctcttccg gcgcaaacca tcggcgagct
     1141 gcgtcaatgt gcgcgcgtgc agagcgctca ccgctccctc actcgatgac cttggggacc
     1201 agatagagcg ccgcagcggt gctcggcgca ttcagctggt agcgctcgtg ggcatcgccc
     1261 tcggtgacct catccgggcg cagccgctgc gtcagacctg ccaggggatg cgccatcggg
     1321 gcgacgtcgg tggtgtcggc gcgctcgagc gcaccgacga actcgaggat gcgcgacagg
     1381 ctttcctggt agacgggcac ctcctcggcc gacagctcga ggcgcgccag tgtggcaatg
     1441 gttgcgatct gctcgcgtgt gagcgccatg ggagccgcgt ggagaactga gaaaaccagc
     1501 gcaaaatcat acatgttgcc ttgctgaatg tcccctcggt tgctagatta gccgccgttt
     1561 ttaggtcgct cctcctggct caatttttcc ggtcgcttca tgctcaaacg cctgcgcggt
     1621 tatttctcca gcgatctgtc gatcgacctc ggcaccgcga acactcttat ttatgtgcgc
     1681 gacaagggca ttgtgctcaa tgagccctcg gtggtcgcgg tgcaggacga gccgacgcgc
     1741 ggaggcaagg tgatcgtcgc cgtcggctcc gaggcgaagg ccatgctcgg acgcacgccc
     1801 ggccacatca ccgccgtgcg tccgatgaag gacggcgtga tcgccgattt cacctacacc
     1861 gagaagatgc tgcagttttt catcaataag gtgcacggca accgcacgct caagccctcc
     1921 ccaagggtgc tggtgtgcgt gcccttcggc tcgacccagg tcgagcgccg cgcgatccgc
     1981 gaatccgccg aaggcgccgg cgcccgcaac gtcgccatcg tgagcgagcc gatggcggcg
     2041 gcggtcggcg cgggactgcc ggtgcacgag gcgcgcggct cgatggtgct cgacgtcggc
     2101 ggcggcacct ccgaagtcgc ggtgctgtcg ctgaatggcg tcgtgtacgc gaactccgcg
     2161 cgcatcggcg gcgatcgctt cgatgaggcg atcatgaact acgtgcggcg caattacggc
     2221 atcctcatcg gcgaggcgac cagcgagcgc atcaagatcg agatcggctc ggcctacccg
     2281 ggccagcagg tgcgggagct ctcggtgaag ggacgcaatc tttccgaagg cgtgccgcgc
     2341 agcttcacgc tcaacagcaa cgagattctc gaggcgctgc aggagccgct gcaggccatc
     2401 gtgagcgcgg tcaagcaggc actcgagcag accccgcccg agctcggtgc ggacgtcgcc
     2461 gagcgcggca tcgtgctcac cggcggcggc gcgctgctgc gcgacatcga caagctgctc
     2521 accgaggaaa ccgggctgcc ggtggtcgtc gcggacgatc ccctcacctg tgtcgcccgc
     2581 ggcggcggac gcatactcga gctgatggac gagctgggtc cgggacactt cggtctcgag
     2641 tagcgcacaa ggagcgcgcg ggttctctta agtggcagcc ttcggcacag gagccggcag
     2701 accgctctcg gggcggggcg gctcccccgg ttttcgcttc accctgtatg cgctgctgtc
     2761 ggtcatcgtg atgtatctcg atcagcgcgc gcattacctc gagcaggtgc gctacgtgtt
     2821 gcaggccacc gcctatccgc tgcagctggc ggtgaattcc ccgacggcgg cctggcgttg
     2881 gctgcaggac accttccgca gccgcgaagc gctgcaggcc gagaacacac ggctgcgcgc
     2941 gaacgcgcgt gatctcgagc tgcgcgccat gcgctacgag gccctggcgc gcgagaacgg
     3001 cgagctgcgc gggctgcgcg aggcgctgcc accggtcgcc gatcgctggc tgccggcgga
     3061 gatcgtgaac atccagctga gcagcctgcg ccagcggctg ctcctcaatc gcggtgccgc
     3121 caacggtgtc ttcaagggac aggcggtgct cgatgacaga gggctgatcg gccagacgac
     3181 tcatgtcgga ccctggagcg ccgaggtgat cctcattacc gaccccgagc acg
//
LOCUS       DATDMP010000620         3233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1098953, whole genome shotgun sequence.
ACCESSION   DATDMP010000620 DATDMP010000000
VERSION     DATDMP010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098953"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2107)
                     /locus_tag="VI195_10920"
     CDS             complement(<1..2107)
                     /locus_tag="VI195_10920"
                     /inference="COORDINATES: protein motif:HMM:NF045328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824944.1"
                     /translation="MTVPEPRIQLLTTPRADDLGLSRLRNPSGLQISLLPNGAIFALE
                     HVEESRRVMVNRCFGSPIAGSMSRLYMRCRGRVAEVVPIAGAPSRCRTAAMGERFRWQ
                     GERSGVAHEVTLQLHGDRPIWLWRVQLHNDTGDTLACDTVLIQDLGLGEPGFLLNNEA
                     YASQYLDHHVAQHPRLGAILMGRQNLAQSGRHPWVVHGCLEGACGYATDFGQLLGPAW
                     RDADDFNIPFGTRLASVRWQHETACAALQSTPATLAPGASACWTFFGAYREDHPQASG
                     AADLAWLASIEESLAAAAAAPGGRSTPSSPPRSLLLEARCAVAESLSEQALHQRYPEQ
                     THQERREGQPLSFFTGAGTDSRHVVLRDKERCVLRRHGALLVSGSHMLPDDRTLCVTC
                     WMHGVFGAQLTLGNTSFHRLWSVSRDPYNITRGSGLRMLVDAGDGWRLLTVPSVFEIG
                     LADCRWIYRLGSGTVTVSAVVSGEEPAAQWRATVEGPPCRFLVLGQVVLGEQELAHAA
                     QLEIDAVRKRFTFRPEPGGFWSQRYPAAVYHLVTSTPASIEAIGSDELLYAARPERSG
                     GYAVLQSAPTNELVFAVVGSLTDPRAAESLAARYAGGIEDSSMRAQALAHWRGVTRSI
                     RIASEVADPAVRALDTSFPWLVHDAMVHFTVPHGLEQYTGGAWGTRDVCQGPLELLLT
                     LEHDAIAKELLRSVFAQQYA"
     gene            2293..>3233
                     /locus_tag="VI195_10925"
     CDS             2293..>3233
                     /locus_tag="VI195_10925"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY6824945.1"
                     /translation="MADTMNGARTRPTAGTHPPPLRADELLESALRKGKRQDFADRSF
                     IRHFEHLLKACNEEADLSSFGRRALRIDVLRCLRNLLYFDRIEAACPGVLSRRILAPV
                     FITGMPRSGTTFLHRLILQDPGNIAPRLFQLVYPYASQAGFGTALRKRWVSLQLALFR
                     LIAPELNALHPVAVESPEECTDITAQVFQSLRFDAMYRVPSYNSWLERNGFLDAYRFH
                     RRFLQHLDAQLPGGRWILKSPDHVFALDDIRKVYPDAHLVLVHRDPVRVLASVAKLTE
                     VLRRPFARSIDRLEIGREVSASWLDGSQRMATLASDAS"
BASE COUNT          544 a         1086 c         1055 g          548 t
ORIGIN      
        1 gcgcgtattg ctgggcaaag acgctgcgca ggagctcctt ggcgatcgcg tcgtgctcga
       61 gcgtcagcaa cagctcgagc ggtccttgac agacatcgcg ggtgccccac gcgccgccgg
      121 tgtactgctc gagcccgtgc ggcacggtga aatgcaccat ggcatcgtgt accagccagg
      181 ggaagctcgt gtcgagcgca cgcaccgcag gatccgccac ttcgctcgcg atgcggatgc
      241 tccgagtcac gccgcgccag tgagcgagtg cctgcgcccg catcgaactg tcctcgatgc
      301 ctcctgcgta gcgggcggcg agcgactccg ctgcgcgcgg gtcggtgagc gagcccacta
      361 ccgcaaagac gagctcattc gtcggcgcgc tctgcagcac cgcatagccg ccgctgcgct
      421 ccgggcgggc cgcgtacagc agctcatcgc tgccgatagc ctcgatgctc gccggggtgc
      481 tcgtcaccag gtggtagacg gcagccggat aacgttgact ccagaatcct ccgggctccg
      541 gccgaaaggt gaagcgcttg cgcaccgcat cgatctcgag ctgcgctgcg tgcgcgagtt
      601 cctgctcacc gagcacgacc tggccaagaa cgaggaaccg gcacggcggt ccctcgacgg
      661 tcgctcgcca ctgcgccgcc ggctcctcac cggacacgac cgccgagacc gtgacggttc
      721 cgctgccgag ccgatagatc cagcggcaat cggccagtcc gatctcgaat acggacggca
      781 cggtcagcaa ccgccagcca tcgcccgcat cgaccagcat gcgcagtccg ctgccgcgtg
      841 tgatgttgta cgggtcgcgg gatacggacc agagccggtg aaaagaggta ttgccgagag
      901 tgagctgcgc gccgaataca ccgtgcatcc agcaggtcac acacagcgtt cgatcatcgg
      961 gcagcatgtg cgatccgctc accagcagcg cgccgtgcct gcgcaacacg caccgctcct
     1021 tgtcgcgcag cacgacgtgg cggctgtcgg tccccgcgcc ggtgaagaag gagagcggct
     1081 gcccctcgcg ccgctcctgg tgcgtctgct ccgggtagcg ctggtgcaac gcctgctctg
     1141 acaagctctc cgcgaccgcg cagcgcgcct cgagcagcaa actgcgtggg ggcgaggaag
     1201 gcgtgctccg gccgcctggc gcggcggccg cggcggccag cgattcctcg atcgatgcca
     1261 gccatgccag gtccgcagcc ccggaagcct gcggatggtc ctcccggtag gcgccgaaaa
     1321 aggtccagca ggcgctggcg ccgggagcga gcgtggcagg tgtcgactgc agtgcggcgc
     1381 aagcggtttc gtgctgccaa cgcaccgagg cgagtcgcgt gccgaagggg atgttgaaat
     1441 catccgcatc acgccaggcg gggcccagca gctgaccaaa gtccgtcgca tagccgcagg
     1501 cgccttcgag acacccgtgc acgacccacg gatggcgtcc gctctgcgca agattctgtc
     1561 gtcccatgag aatcgcgcct agccggggat gctgtgcaac atggtggtcg agatactggc
     1621 tcgcataggc ttcgttgttg aggaggaaac cgggttctcc gagccccagg tcctgaatca
     1681 gaacagtgtc gcaggcaagg gtgtcgccgg tgtcgttgtg cagctgcacg cgccaaagcc
     1741 agataggccg gtcgccgtgc agttgcagtg tcacctcgtg cgcaactcca cttcgctctc
     1801 cctgccagcg gaagcgctca cccatggcag cggtgcggca gcgcgagggt gcgccggcga
     1861 tcggcacgac ctcggctacg cggccgcggc agcgcatgta cagacgactc atgctgccgg
     1921 caatcggcga gccgaaacac cggttgacca tgacccggcg gctctcttcc acgtgctcca
     1981 gggcgaagat ggcgccattg ggaagaaggc tgatctggag gccggacggg ttgcgcaggc
     2041 ggctgagccc gaggtcatcc gcgcgcgggg tggtcagaag ctgaatccgg ggttcaggta
     2101 cggtcattac tgcgcgcgca tcatgagcga catactaacg cggccccatt ccccctcggg
     2161 gagccaacgc cttggccttg agcgcgtcgg gtcgccgctg gcaccccttc aggcagctcg
     2221 ggggtagccc tttcgtccta gtgcgctgcg tcgcgtagaa gcgatatggt tcggattttg
     2281 ccgggaacgt ctatggcaga cacaatgaac ggcgcgcgca cacgtccgac cgcggggacg
     2341 catccacccc cgctgcgcgc ggacgagctg ctggaatccg cactgcggaa aggcaagcgg
     2401 caggacttcg ccgaccgctc cttcattcgc cacttcgagc acctgttgaa ggcctgtaac
     2461 gaagaggcgg atctgagctc cttcggtaga cgtgcgttaa ggatcgacgt gctgcgctgc
     2521 ctgcggaatc tgctgtattt cgatcgcatc gaggccgcct gccctggcgt actgtcgcgc
     2581 cgcatcctcg caccggtgtt catcaccgga atgccgcgca gcggcaccac cttcctgcac
     2641 cgcctgattc tgcaggaccc gggcaacatc gcaccgcggc tgtttcagct cgtgtacccg
     2701 tacgcgtcgc aggccgggtt cggcacggcg ctgcgcaagc gctgggtcag tctgcagctc
     2761 gctctgttcc gcctgatcgc cccggagctc aatgcccttc acccggtcgc ggtcgaatcg
     2821 cccgaggagt gtaccgacat caccgcgcag gtttttcaga gcctgcgttt cgatgccatg
     2881 taccgcgtgc cctcctacaa cagctggctc gagcgcaatg gctttctcga tgcctatcgc
     2941 tttcatcggc gttttctgca gcacctcgac gcccagctcc cgggaggacg atggattctc
     3001 aagtctcccg atcacgtgtt tgcgctcgat gacatccgca aggtctaccc cgatgcgcat
     3061 ctggtgctgg tccatcgcga tccggtgcgt gtacttgcct cggtagcgaa actgaccgag
     3121 gtgctgcgcc ggccgttcgc gcgcagcatc gatcgactgg agataggccg cgaggtcagc
     3181 gcttcgtggc tggacggctc gcaacggatg gcgacactgg ccagtgacgc ttc
//
LOCUS       DATDMP010000621         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_320927, whole genome shotgun sequence.
ACCESSION   DATDMP010000621 DATDMP010000000
VERSION     DATDMP010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320927"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VI195_10930"
     CDS             <1..478
                     /locus_tag="VI195_10930"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY6824946.1"
                     /translation="ARGVRVVPATHFAEGGRGALELAREVVQLTEQPSRFRFVYSDNA
                     TLWDKMKAIATEIYHASDITADTKVRAQIRQLQEGGYGHYPVCVAKTQYSFSTDPKLR
                     GAPAHHVVNVREVRLAAGAEFVIMICGDIMTMPGLPKVPAANNIDVDSDGRITGLF"
     gene            complement(518..1195)
                     /locus_tag="VI195_10935"
     CDS             complement(518..1195)
                     /locus_tag="VI195_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824947.1"
                     /translation="MNLPWRGGRQIALAILGVSVACQVARGAEASKVMGSIEVAAGEH
                     AGDVSTVNGSIHVHENAVVGTAHAVNGSISLEARASAAELTSVNGSIELAPQVKVSGK
                     VQTVNGKLSLADGAEVGGSLVNVNGAIHVAAAHVGGGIETVSGGVELGPSAHIDGGIH
                     VRKPGGFFNSLGLEDVPRIIIGPGSVVGGTLEFERTVKLYVSDEAKIGAVQGATAVKY
                     SGERPPE"
     gene            1245..1991
                     /locus_tag="VI195_10940"
     CDS             1245..1991
                     /locus_tag="VI195_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY6824948.1"
                     /translation="MSAKKKGPRAPDRRHVLVIDVGGTHVKFRIGVRGAVQEFDSGPK
                     MTPARMTRHLRKLLGGQRYDAVSIGYPGLVYHGRIAAEPHNLGNGWVGYDFDAALGRA
                     VRVINDAAMQAIGSYRGGRMLFLGLGTGLGATLIIDGIVEPTEVGHMPYKHGRTFEDY
                     VGEHGRKRLGNRRWRKVVRKVIATLSAAFEADYIVLGGGNARRLMKLPPGVRLGSNLH
                     AFVGGLRLWDPSSGIITSPSAAVRAARRRR"
     gene            1988..>3304
                     /locus_tag="VI195_10945"
     CDS             1988..>3304
                     /locus_tag="VI195_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY6824949.1"
                     /translation="MSTAPGAPGLSPTWCSSDKEMVGCTLGASKLWFTIGGGIINEVY
                     YPRVDLPQIRDLGFIVGDGTAFWVEVKRLWRHTVQLAAPGAPAVRIVHRHERFELTLR
                     VTPCESRDVLLIEVSLSGDETLKPYALLAPHLGGTGLGNRAAVADYRGRRLLWAEQGP
                     FGLALAAVTAQQQEAFGRASAGVVGASDGWQDFARNGRLTWQYDDAGPGNVALIGELP
                     RQAVLALGFGSSPESAATLALSALSEPFAISWERQLTAWTSWHTPCAHDVLCMGLPAE
                     CAEQVRISTMVLRVHQDQTYPGALVASLSVPWGNTSEERPGYHLVWPRDLVESAGALF
                     AVGALPEARNTLRYLQATQHADGHWSQNQWLGGKAYWEGLQLDETAFPVLLAVALDEH
                     NALEGTEVRDMIQRALAFLIRNGPVTQQDRWEENAGLNTFTLALCIS"
BASE COUNT          531 a         1117 c         1107 g          549 t
ORIGIN      
        1 cgcgcgcggc gtgcgggtcg tgccggcgac gcactttgcc gagggtggtc gcggcgcact
       61 ggaactggcg cgcgaggtgg tgcagctcac cgagcaaccc agccgcttcc gcttcgtcta
      121 ctccgacaat gcgactttgt gggacaagat gaaagccatc gccaccgaga tctatcacgc
      181 gagcgatatc accgccgaca ccaaggtacg ggcgcagatc cgtcagctgc aggaaggcgg
      241 gtacggccac tatccggtgt gcgtcgcgaa gacgcagtac tcgttctcca ccgacccgaa
      301 gctgcgcggt gccccggcgc accacgtagt caacgtccgc gaggtgcggc tcgcggccgg
      361 ggccgagttc gtgatcatga tctgcggcga catcatgacc atgcccgggc tgccgaaggt
      421 gccggcggcg aacaacatcg acgtcgacag cgacggacgc atcaccggac tcttctaggc
      481 gcggcggcgc ctgattgccg gaaagcagca tcgaggctca ctccggcggc cgctcacccg
      541 agtacttgac cgcggtcgca ccctgcacgg caccgatctt cgcctcgtcg ctcacgtaga
      601 gcttgacggt gcgctcgaac tcgagcgtgc caccgacgac cgagcccgga ccgatgatga
      661 tgcgaggcac atcctcgagc ccgaggctgt tgaagaagcc gccgggcttg cgtacgtgga
      721 tgccaccatc gatgtgcgcg ctcggcccga gctcgacgcc gcctgacacc gtctcgatgc
      781 cgccgccaac gtgagcggcc gcgacgtgaa tggcgccgtt gacattcacc agcgagccgc
      841 cgacctcggc gccatcggcg aggctcagct tgccgttcac cgtctggacc ttgccgctca
      901 ccttcacctg cggcgcgagc tcgatcgagc cgttaacgct cgtcagctcc gcggcgctcg
      961 cgcgtgcctc gagtgagatg ctgccgttga ccgcgtgcgc cgtgcccacg accgcgttct
     1021 cgtgcacgtg gatcgagccg ttcaccgtcg agacatcccc ggcgtgctcg cccgcggcca
     1081 cctcgatgct gcccatcacc ttggaggctt ccgcgccccg tgccacctga catgccacgc
     1141 tcacgccgag gatcgccaac gcgatttgcc gaccgcctcg ccatgggaga ttcatgcgct
     1201 tacctcaggt ggtcaaggcc acgcaatcca tggagaatcg cccgatgtcc gcaaaaaaga
     1261 aaggcccgcg tgccccggac cgcaggcacg tcctggtgat cgatgtcggt gggacacacg
     1321 tgaaattccg catcggcgtg cgcggtgccg tccaggagtt cgattccggt ccgaagatga
     1381 cgccggcgcg tatgacgcgt cacctgcgca agctactggg agggcaacgt tatgatgccg
     1441 tctccatcgg gtatccgggc ctggtgtacc acgggcgcat cgccgccgag ccgcacaacc
     1501 tcggcaatgg ctgggtaggc tatgactttg acgccgcgct cgggcgagcg gtacgggtga
     1561 tcaacgatgc ggccatgcag gccatcggca gctatcgcgg cggccgcatg ctctttctcg
     1621 ggctcggcac cgggctcggc gccacgctca tcatcgatgg gatagtcgag ccgaccgagg
     1681 tcggtcacat gccctataag cacggccgca ccttcgagga ctacgtcggc gagcacggtc
     1741 gcaagcgcct cggcaaccgc cgctggcgca aggtggtgcg caaggtgatc gcaactctca
     1801 gtgccgcttt cgaagcggac tacatcgtgc tcggcggcgg caatgccagg cggctgatga
     1861 agcttccgcc aggtgtccga ctcgggtcca atctgcacgc ctttgttggc gggctgcggc
     1921 tgtgggatcc gagctcgggc atcatcacct cgccatccgc tgcggtgcgt gcggcgcgcc
     1981 ggcggcgatg agcacagcgc ccggagctcc cggactctcg ccgacctggt gcagcagcga
     2041 caaggagatg gtcggctgca cgctcggcgc atcgaagctg tggttcacca ttggcggcgg
     2101 aatcatcaac gaggtgtatt acccgcgcgt cgaccttcct cagatccgtg atctcggttt
     2161 catcgtgggc gacggcacgg ccttctgggt cgaggtgaag cggctgtggc ggcacaccgt
     2221 gcagcttgcc gcgccgggtg ctccggcggt gcgcatcgtc catcgtcacg agcgtttcga
     2281 gctcacgctg cgcgtaacgc cgtgcgagag ccgcgacgta ctgctcatcg aggtgagcct
     2341 gagcggtgat gagacgctga agccgtacgc actgctcgct ccgcacctcg gcggcaccgg
     2401 cttgggcaac cgcgccgcgg tggccgacta ccgcgggcgg cgcttgctgt gggcggagca
     2461 gggccccttt ggtctcgcgc tggcggcggt caccgcgcag cagcaggagg cattcggacg
     2521 cgccagcgca ggtgtggtcg gcgcgagcga cggctggcag gactttgccc gcaacggccg
     2581 cctcacctgg cagtatgacg atgccggacc cggcaacgtc gcgttgatcg gtgagctgcc
     2641 gcgtcaagcg gtgttggcgc tcggcttcgg cagcagtccc gagtcggctg cgacgctcgc
     2701 gctcagcgcg ctctcggaac ccttcgcgat ctcctgggag cgccagctca ccgcctggac
     2761 gagttggcac accccgtgtg cccacgatgt gttgtgcatg ggcttgcctg cggagtgtgc
     2821 cgagcaggtg cgcatctcca ccatggtgct gcgcgtccac caggatcaga cctatccggg
     2881 cgcgctggtc gcctcgctca gcgtgccctg gggaaacacc agcgaggagc ggcccggcta
     2941 tcacctggtg tggccgcgcg acctggtcga gagcgccggt gcgctgttcg ccgtcggcgc
     3001 gctgccggaa gcgcgcaaca cgctgcgcta cctgcaggcc actcaacatg ccgacggtca
     3061 ctggagccag aatcagtggc tcggcggcaa ggcgtactgg gagggactgc agctcgatga
     3121 gaccgccttt ccggtgctgc tcgcggtagc gctcgatgag cacaacgcgc tcgagggcac
     3181 cgaggtgcgt gacatgatcc agcgcgcgct cgcgttcctg atccgtaacg ggccggtgac
     3241 ccaacaggat cgctgggagg aaaacgcggg gctgaacacg ttcacgctcg ctctgtgcat
     3301 ctcg
//
LOCUS       DATDMP010000622         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_432174, whole genome shotgun sequence.
ACCESSION   DATDMP010000622 DATDMP010000000
VERSION     DATDMP010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432174"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VI195_10950"
     CDS             <1..744
                     /locus_tag="VI195_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY6824950.1"
                     /translation="FKPEIIMVTPSYMLAIADEMQRQGLDPSSTSLRIGIFGAEPWTN
                     EMRATIEKRTGLQAIDLYGLSEVIGPGVAQECLETKDGLTIWEDHFFPEVIDPQTGEP
                     LPDGEKGELVFTSLTKEALPVIRYRTRDLTRLLPGTARAMRRMEKITGRSDDMLIIRG
                     VNVFPSQIEELLLKQPGLTPHYVLELSKEGPLDHLAVLVEGAAEAAAALGHHIKSTIG
                     ISAEVKTVAAGSIERSIGKAKRVIDRRPK"
     gene            741..1643
                     /locus_tag="VI195_10955"
     CDS             741..1643
                     /locus_tag="VI195_10955"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY6824951.1"
                     /translation="MKPLCARGALTGRKNRIAPQSYRPENAFMELIEKARSYLDARRG
                     ERVTLQELAGAVGASPFHVQRRFKETFGVSPRGYQEAQRVEAVKSSLRNGSRVTQAVY
                     DAGYGSVSRFYEKAASRLGMDASEYRARGKGKRIVFCTFASPLGTVLVGATERGLCSV
                     KIGNQAKPLARLLREEFSEAELVENPGSLRAIRAAVLAFLAGDRKIARLPLDIRGTVF
                     QRRVWEALRTIPRGETRTYAQLARAIGAPAAVRAVGSACGANPVALVIPCHRAVRTDG
                     GLGGYAWGLDRKRQLLKLEKGNKK"
     gene            1640..1852
                     /locus_tag="VI195_10960"
     CDS             1640..1852
                     /locus_tag="VI195_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824952.1"
                     /translation="MKLVRRILVALFPIALAACGVETAGTAATAAAVKKQEIEQGKNA
                     MRDAQVKIDAAAKKVEASAAASDSEK"
     gene            1865..2713
                     /locus_tag="VI195_10965"
     CDS             1865..2713
                     /locus_tag="VI195_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6824953.1"
                     /translation="MRRADATRLVILAALWGASFLFMRVAAPVLGPFIVADVRSLLAA
                     AALAVYFRYTGFSSDWRRWWRDYLLIGTINTALPFALYAYAAVHIPASVSAVTNATAP
                     IWGALLATVLLRERLTAARAAGLLLGIVGVALVTRPPASVTYPALAIAAAVSGAVCYA
                     VAGVYLKARPRQAPGRGMAFGTQLASGLLLMPCAAFSQPLAPVTPLAIACTLAMALLS
                     GAVAYVLYFRLIADIGPIRSLTVTYLIPLFGVLFGAVFLGEAVNATMLGGGALVVLGT
                     VLVLRK"
     gene            2719..>3193
                     /locus_tag="VI195_10970"
     CDS             2719..>3193
                     /locus_tag="VI195_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEY6824954.1"
                     /translation="MQYPHLFRELDLGFVRLKNRLLMGSMHTGFESRSDGIERLAAFY
                     AERARGGAALIVTGGYSPDDAGNLGPHRAQFSSPQDRDAHRPIPAAVHAAGSLIVLQL
                     LHSGRYGFHERIVAPSAVKAPINAQAPRELSAAEIEQTIEDFASAARLAREAGYDG"
BASE COUNT          550 a         1084 c         1016 g          543 t
ORIGIN      
        1 ttcaagcccg agatcatcat ggtgacgccg agctacatgc tcgccatcgc cgacgagatg
       61 cagcgccagg gcctcgaccc ctcgagcact tcgcttcgca tcggcatctt cggcgccgag
      121 ccgtggacca atgaaatgcg cgccaccatc gagaagcgca ccgggctgca ggcgatcgac
      181 ctctacggcc tgtccgaagt catcgggccg ggcgtggcgc aggaatgcct cgaaaccaag
      241 gatgggctga cgatctggga agatcatttt tttcccgagg tcatcgaccc gcaaaccggg
      301 gaaccgcttc ccgacggcga aaagggggag ctcgtcttca cctcgctcac caaggaagcg
      361 cttcccgtaa tccgttaccg gacgcgcgat ctcacgcgcc tgctgccggg caccgcccga
      421 gccatgcgcc gcatggaaaa gatcaccggc cgctccgacg acatgctcat catccgcggc
      481 gtcaacgtgt ttccatcgca gatcgaggag ttgctgctga agcaaccggg gctcacgccg
      541 cactatgtgc tcgagctctc caaggaaggc ccgctcgacc atctcgccgt gctcgtcgaa
      601 ggcgcggccg aggctgccgc ggcgcttggc catcacatca agtcgaccat cggcatcagc
      661 gcggaagtga aaaccgtcgc cgcggggagc atcgagcgct ccatcggcaa ggcgaagcgc
      721 gtcatcgatc gcaggccgaa atgaagcccc tctgcgcgcg gggcgcgctc accgggcgca
      781 agaaccggat agcgccgcag tcctatcggc ccgaaaatgc cttcatggag ctcatcgaaa
      841 aggcaagaag ctacctggac gcccgccgag gcgagcgcgt cacgctgcaa gagctggccg
      901 gcgccgtcgg tgcaagcccc tttcacgtgc aaaggcgctt caaggagacc ttcggggtct
      961 cgccgcgcgg ctaccaggag gcgcagcgcg tggaagcggt gaaatcgtcc ctcagaaacg
     1021 gaagccgcgt cacccaggcc gtgtacgacg ccggctatgg atcggtctcc cggttctacg
     1081 aaaaagccgc ttcccgcctg ggcatggacg cttcggagta ccgcgccaga ggcaagggca
     1141 agcgcattgt cttctgcact ttcgcatcgc ccctcggcac ggtcctcgtc ggggccaccg
     1201 agcgcggtct gtgcagcgta aagatcggca atcaagcgaa gccgcttgcg cgcctgctgc
     1261 gcgaggaatt cagcgaggcg gagctcgtgg agaaccccgg gtcgctgcgc gccataagag
     1321 cggcggtgct cgctttcctc gccggggaca ggaaaattgc gcgcctgccg ctcgacatcc
     1381 gcggcacggt gttccagcgc cgggtatggg aggcattgcg caccatccct cgaggcgaga
     1441 cacgtactta tgcccagctc gcgcgcgcga tcggcgcccc cgctgcagtg cgcgccgtgg
     1501 gcagcgcgtg cggcgcaaac ccggtcgccc tcgtgatacc ttgtcaccgc gccgtgcgaa
     1561 ccgacggcgg cctgggcggc tatgcctggg gcctggatcg caagcggcag ctgctcaagc
     1621 ttgaaaaagg aaacaagaaa tgaagctcgt ccgccgaatc ctcgtagccc tttttcctat
     1681 tgcgctcgca gcgtgcggcg ttgagaccgc aggcactgca gccactgcgg cggcggtaaa
     1741 aaaacaggaa atcgagcagg gcaagaacgc catgcgcgac gcgcaggtaa agatcgacgc
     1801 ggctgcgaag aaagtcgaag cgagcgctgc ggcgagcgac agcgaaaagt aactctcctg
     1861 ccgcatgagg cgcgccgacg ccacgcgcct cgttatcctt gccgcccttt ggggcgcttc
     1921 ctttctcttc atgcgcgtcg cggccccggt ccttggccct ttcatcgtag ccgacgtgag
     1981 gagcctcctg gccgctgccg cgctggcggt ctacttccgg tacaccggct tcagtagcga
     2041 ttggcgacgc tggtggcgcg actacctcct gatcggaacc ataaacacgg cgttgccatt
     2101 cgccttgtac gcctatgcgg cggttcatat cccggcgagc gtatcggccg tcaccaatgc
     2161 cacggcaccg atatggggag ccctgctcgc cacggtcttg ctgcgcgaac gcctcaccgc
     2221 agcacgcgca gccggattgc tgctcggtat cgtcggtgtt gcgttggtga cgcgcccgcc
     2281 tgcaagcgtg acctacccag cgctagccat cgctgccgcg gtcagcggag ccgtgtgcta
     2341 tgccgtggcg ggcgtctacc tcaaggcacg gccgcggcag gcaccggggc gcggaatggc
     2401 gtttggcacc cagctcgcct cgggcctgct gctcatgccg tgcgcggcat tctcgcaacc
     2461 gctcgcgcct gtcacgccgc tcgcgattgc ctgcacgctc gcgatggcgc ttttaagcgg
     2521 cgccgtggcc tatgtgctgt atttccgcct gatcgccgac atcggcccga tccgctccct
     2581 gaccgtgacc tacctgatcc cgcttttcgg cgtcctcttc ggtgcggttt tcctcgggga
     2641 agcggtgaat gcgacgatgc tcggcggcgg cgcgctcgtg gtcctgggca ccgtgctcgt
     2701 tctgcgaaaa taggacccgt gcagtatccg cacctttttc gcgagctgga tctcggcttc
     2761 gtaaggctga agaaccgctt gctcatgggc tcgatgcata ccggcttcga gtcgcgaagc
     2821 gatggcatcg agcgtctcgc ggcgttctat gccgagcgcg ccaggggcgg cgcggcgctc
     2881 atcgtcactg gcggatattc gccggacgat gcgggcaacc taggaccgca ccgcgcgcaa
     2941 ttctcgtcgc cgcaggatcg cgatgcgcac cggccgattc ccgcggctgt gcacgccgcc
     3001 ggcagcctga tagtgctgca gctgctgcat tccggccgct acggcttcca cgagcgcatt
     3061 gtggcgccct ccgccgtgaa ggccccgatc aatgcccagg cgccgcgcga gctctccgca
     3121 gccgaaatcg agcagacgat cgaggacttc gcttctgcag cccgccttgc ccgcgaagcc
     3181 ggctacgacg gtg
//
LOCUS       DATDMP010000623         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_432240, whole genome shotgun sequence.
ACCESSION   DATDMP010000623 DATDMP010000000
VERSION     DATDMP010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432240"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VI195_10975"
     CDS             <1..567
                     /locus_tag="VI195_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6824955.1"
                     /translation="LKPESSTGWDAGFEQGLAHGAVRFGATYYYNRIHDLITSDVTGT
                     TYANIGEATTDGIESFIAWQPLTPLTLRLDYTYTEATDEVLHQELLRRPKHKGTVLAQ
                     WQVTSAWQLSVDVLAVGSWVDGNRDFSIPRLDAPGYVTVDLATSYELNQHLAVLGRIN
                     NVLDRRYENPVGFQQPGIGAYAGLRLTL"
     gene            592..987
                     /locus_tag="VI195_10980"
     CDS             592..987
                     /locus_tag="VI195_10980"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY6824956.1"
                     /translation="MDGPNSTATPAAAPTENAPTENERTWGMLAHLSALTGLVLPLAG
                     IVIGPLVVWLARRDDSEFVAAHAKEALNFNISVLLGALACMLLMLVFIGFLLGTALFI
                     AWLVMTLIAAIKASEGHPYRYPFSLRLVK"
     gene            1340..>2396
                     /locus_tag="VI195_10985"
     CDS             1340..>2396
                     /locus_tag="VI195_10985"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY6824957.1"
                     /translation="MRHSIIGRRSVTGRGALSNPPGRFDKQQLAEVDDGWYQDEVPES
                     IPTTLEPDRARSVIATNDSPDIPFEQSINPYRGCSHGCVYCYARPSHAYIGLSPGLDF
                     ETRLFYKADAAKMLEAQLARPGYLCKSITLGANTDPYQPVERSMKVSRAILEVLRRTR
                     HPVAIITKSALVLRDLDLLTDLARDRLASVAVSVTTLDPELKRVMEPQAASPQARLRT
                     LAALAAAGIPTSVMAAPMIPALNDQELESILEAAAAAGARWAGYVLLRLPYEIKDLFR
                     EWLAQHYPDRAAHVMSLIRDMRGGRDYDSSFGTRMRGTGPYAQLLSSRFRLACRRLKL
                     NAEPREPLNTALFRPPAP"
BASE COUNT          414 a          805 c          731 g          446 t
ORIGIN      
        1 ctcaagcccg agagcagcac cggctgggac gccggcttcg agcaggggct cgcgcacggc
       61 gcggtgcgct tcggcgcgac ctactactac aaccgcatcc acgacctgat caccagcgat
      121 gtgaccggca cgacctacgc caacatcggc gaggcgacca cggatggcat cgagagcttc
      181 atcgcctggc aaccgctcac gccgctgaca ctgcgcctgg attacaccta cacggaagcc
      241 accgatgagg tactgcacca ggagctgctg cgccggccga agcacaaggg gactgtgctc
      301 gcacagtggc aggtcaccag cgcctggcag ctgagcgtcg atgtgctcgc agtcggctcc
      361 tgggtcgatg gcaaccgcga cttctccatt ccacgcctcg atgcgcccgg ttacgtgacc
      421 gtcgatctcg ccaccagcta cgagctcaat cagcatctcg ccgtgctcgg gcggatcaac
      481 aacgtgctcg accggcgcta cgaaaacccg gtcggctttc agcagccggg tattggcgcc
      541 tatgcgggcc tcaggctgac gttgtagtag tgataatctt gggtccgtcg tatggacggc
      601 ccgaactcca ccgccacccc ggctgctgcg ccgacggaga atgcgccgac ggagaacgaa
      661 cgcacctggg gcatgctggc gcatctctcc gccttgaccg gcctcgtgct gccgctcgcc
      721 ggtatcgtca tcgggccgct ggtggtgtgg ctggcgcggc gcgacgattc ggaattcgtc
      781 gcggcgcatg ccaaggaagc gctcaacttc aatatttcgg tgttgctcgg ggcgcttgcc
      841 tgcatgttgc tgatgctggt gttcatcggt ttcctgctcg gtaccgcgct ctttatcgcg
      901 tggctcgtca tgacgctcat cgcggcgatc aaggcgagcg aggggcaccc ctaccgctat
      961 ccgttttccc tgcgcctggt gaagtagtcg ggcgccgtca ccccaccggc gactcgtagc
     1021 gcgcggatgc acggctaccg tcgccccgtt gccaggcacc cctgccgggc ttttcgagat
     1081 tcccaacaac gcatgccagc ggtctcgcag aatgtgtccc tttcggggcc atgcgcatga
     1141 ggcatgcgca tcgctcatgc gcatagttga tgcgcatcgc tcatgcgcac gggttatgcg
     1201 cataggccat gcgcatgacc ccggacacat accctgccat cgctctcgga tctttggtct
     1261 gagcgagtcg ttgagagccc taacccgctg ttttcattgt tctatacgac ctatatatgt
     1321 ataggtgtac agtaaccgca tgcgccacag catcattggc cgacgttctg ttacgggccg
     1381 aggggccctg tcgaacccac ccgggagatt cgacaagcag cagctcgccg aggtcgatga
     1441 cggttggtac caggacgagg tgcccgagag cattccgacg acgctcgagc cggaccgggc
     1501 gcgctccgtg atcgccacga atgattcgcc cgatattcca ttcgagcagt ccatcaatcc
     1561 atatcgtgga tgctcccatg gatgcgtcta ttgctacgcc cggccgagcc atgcctacat
     1621 cggcctttcc cctgggctcg actttgagac gcgtctcttc tacaaggccg acgcggccaa
     1681 aatgctggag gcgcaactgg cccgtcccgg gtacctctgt aagtccatca ccctcggtgc
     1741 caacaccgat ccgtaccagc ctgtcgagcg gagcatgaag gtcagcaggg caatcctcga
     1801 ggtcctgcga cgcacgcgtc atccggtcgc catcatcacc aagagcgcgt tggtgctgcg
     1861 cgacctcgat ttgctgacag acctggcacg cgatcgcctc gcgagcgtcg ccgtcagtgt
     1921 cacgaccctc gacccggaac tgaagcgggt gatggaaccg caggccgcat ctccacaagc
     1981 gcgactgcgc acgctcgccg cgctggcggc agccggcatc ccgacgagcg tcatggcggc
     2041 accgatgatc ccggccttga acgaccagga gctcgagtcg atcctcgagg cggcggcggc
     2101 cgctggcgcg cgctgggccg ggtatgtgct gctgcggctg ccctacgaaa tcaaggatct
     2161 gttccgcgag tggctcgcgc agcactaccc cgaccgggcg gcacacgtca tgtcgctgat
     2221 tcgcgatatg cggggcggcc gcgactacga ttcgagcttc ggtacgcgaa tgcgcggcac
     2281 ggggccgtac gcgcagttgc tgagtagccg cttccggctt gcctgccgcc gcctgaaact
     2341 gaacgccgag cctcgtgagc ccctgaatac ggcgttgttc cgtccgcctg cacctg
//
LOCUS       DATDMP010000624         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_99141, whole genome shotgun sequence.
ACCESSION   DATDMP010000624 DATDMP010000000
VERSION     DATDMP010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99141"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1481)
                     /locus_tag="VI195_10990"
     CDS             complement(81..1481)
                     /locus_tag="VI195_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824958.1"
                     /translation="MSFAIPSTHATRWWPLYLAAAATFLPALGFYYVGEEAIFPISSL
                     EMWYHGKWIEQLFYGSSLKHNPLFNWMIIPLASLAGWEHMLAVARALTIAATVGTGLV
                     VAWLCKALYRDPAFAAFAALVYLTLADIFFYRGWLAYADPLFAFFLMTAVACLWIACL
                     RQSVRLLALAVFALTCAFMTKALTAYVFYGGAALVLLLTERQHRVFLLGPGSWVAHVF
                     GAAAAVGWLYSLPQNSGQGGRMFAEIGAKLAPTDLADYLVKLVAYPFDTLLRLGPLAL
                     VAAYYFWKGRNVQDGDPHVRTALAIALVNYLPYWLAPQSSIRYLLPLYPLAALAIARW
                     LWTRGGSITARRWIIALVAVKLVFVLAAFPYYQSRYRGENFEAAARDIARRTAGHPLY
                     TNNVSASGMSVTAHVDILRLPAAPLTFAPERWDTGFVMAYAPDPELGRIAGEYRLGGD
                     TLYLLCRGAACGERHP"
     gene            complement(1518..>2097)
                     /locus_tag="VI195_10995"
     CDS             complement(1518..>2097)
                     /locus_tag="VI195_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824959.1"
                     /translation="QIFLEPAGPEYRCPDGTPARDFDVVFRGMCNGFRYDGSATFADQ
                     KRLDAARAQALLATAASGSEQYAAALRRGQGEPNLALLAHLARLAHRLEGQGGTLVVM
                     LPPLLPGMEEALARAPHSGPALRATKEVIEGWSTREHVVLLDAGRSERYGCIGTEFID
                     RHHALPECYRKVFSRFFANRAALQTTQAAPSR"
BASE COUNT          427 a          670 c          667 g          333 t
ORIGIN      
        1 acgactcgcg aaatagtcgg cgacgatctc catgtagccg gtgaaattgg ccacatggct
       61 gaattcccgc cattgtccgc tcatgggtgc ctctccccgc acgctgcgcc gcgacacagc
      121 aaatacagcg tatcgccgcc aagtcggtat tccccggcaa ttcgccccag ctccggatcg
      181 ggcgcgtacg ccattacgaa gccggtatcc cagcgctcgg gcgcgaacgt cagcggtgcc
      241 gccggcaggc gcaggatatc gacatgtgcg gtcacgctca tcccgctcgc gctgacgttg
      301 ttggtgtaga gcgggtgtcc cgcggtgcgg cgcgcaatgt cccgcgccgc cgcctcgaaa
      361 ttctcccccc ggtagcggct ctgatagtac gggaaagctg cgagaacgaa aacgagcttt
      421 accgctacca gggcaatgat ccagcgtcgc gcggttatcg agcccccacg cgtccatagc
      481 cagcgtgcga tcgcaagagc tgcaagcggg tacagcggca acaggtaacg tatcgagctc
      541 tgtggcgcga gccagtacgg caaatagttc accaatgcga tggccagggc ggtgcggacg
      601 tgcgggtcac cgtcctgtac attgcgtccc ttccaaaagt aatacgcggc aaccaacgcc
      661 agcggaccca ggcgcagcag tgtatcgaaa ggatatgcga cgagtttgac caggtagtcc
      721 gcgagatccg tcggtgccag tttggcaccg atttcggcga acatgcgccc gccctgtccg
      781 gaattttgcg gaagagaata aagccacccc accgccgcag ccgcgccgaa tacgtgcgca
      841 acccacgatc ccgggcccag caggaacacg cgatgttggc gctcggttaa caacagcacg
      901 agagccgcgc ctccataaaa tacataggcc gtcagcgcct tggtcatgaa agcgcaagtc
      961 agcgcgaata ccgccaacgc gagcaacctc acgctctgcc gcaggcacgc aatccagaga
     1021 caagccaccg cggtcatcag gaaaaacgcg aacagggggt cggcataggc cagccatccg
     1081 cgatagaaaa aaatgtcggc gagcgtgagg tagacgagcg cggcgaatgc ggcgaaagcc
     1141 ggatcccgat agagcgcctt gcacaaccat gcaacaacga ggccagtgcc aaccgttgcc
     1201 gcgatggtca gcgcccgcgc caccgccagc atatgctccc agccggcaag actcgcgagc
     1261 gggatgatca tccagttgaa aagcggattg tgcttgaggc tcgatccgta gaacagttgc
     1321 tcgatccact tgccgtggta ccacatttcg agcgaggaga tcgggaagat cgcctcttcg
     1381 ccgacgtagt agaagccgag cgcgggcagg aacgtcgcgg cggcagcgag gtagagaggc
     1441 caccatcggg tggcatgggt cgaggggatc gcgaagctca cgggtcgtgg ggtggcgccg
     1501 caacgggcgc ggctattcta tcgactcgga gccgcttgcg tcgtctgcag cgccgcgcgg
     1561 ttggcgaaga agcgtgaaaa taccttgcga tagcactccg gcagggcatg atggcggtcg
     1621 atgaattccg ttccgatgca gccgtagcgc tcggagcgcc cggcatcaag cagcacgacg
     1681 tgctcgcgcg tgctccagcc ctcgatcact tccttggttg cgcggagcgc tgggccgcta
     1741 tgcggggccc gggccagcgc ttcctccatg ccgggcagca gcggtggcag catcacgacc
     1801 agcgttccgc cctgcccctc gaggcggtgc gctagacgcg caaggtgagc gagcagcgcc
     1861 aggttcggtt cgccctgccc tcgccgcaat gcggcagcgt actgttccga cccggatgcg
     1921 gctgtagcca gcagcgcctg cgcccgtgct gcgtcgaggc gcttctgatc agcgaaggtt
     1981 gcgctcccgt cgtagcgaaa gccattgcac attccgcgaa acaccacgtc gaagtcgcgt
     2041 gccggcgtcc cgtccgggca acggtactcg ggcccggccg gttcgaggaa tatctgg
//
LOCUS       DATDMP010000625         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1432394, whole genome shotgun sequence.
ACCESSION   DATDMP010000625 DATDMP010000000
VERSION     DATDMP010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432394"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..348
                     /locus_tag="VI195_11000"
     CDS             76..348
                     /locus_tag="VI195_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824960.1"
                     /translation="MSEKPESDEFEDEEADEPVIEDGDVDLDQVIKDLDLAKRRGQKA
                     GDPAWRRLERLLEEKHTAELTSDFEDYEIGDEGEPPGRKARRKPRS"
     gene            complement(434..>3104)
                     /locus_tag="VI195_11005"
     CDS             complement(434..>3104)
                     /locus_tag="VI195_11005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY6824961.1"
                     /translation="DRVRIAVELLRTGAHGRRLATLAGIGAECGIGLIASGDVHMHVR
                     GRRRLQDALTAIRLNEPVAQLGTRLYANGERYLRERERLGRLYPRELLAATIELAQSC
                     HFSLDELRYEYPRELVPPGETPTSYLRRLTQAGARWRWPDGVPAAERTAIEHELALIE
                     ELHYESYFLTVQDIVAYARRQGILCQGRGSAANSRVCYCLGVTSVDPQRGASLLFERF
                     ISRERNEPPDIDIDFEHERREEVLQYVYNKYGRERAALAATVIMYRPRSALRDLGKVF
                     GLTPEQSGRLAKVMQWWDGSATIPARVREAGFHADSPWLRRLLSLACELTAFPGFPRH
                     LSQHVGGFVIAQGALQELVPIENAAMPERTVVQWDKDDLNDLGLLKVDLLALGMLTAL
                     KRAFALVNDYRGTRYTLGELPAEDPKVYDMVCRADTIGVFQIESRAQMAMLPRLKPRC
                     YYDLVIEVAIVRPGPIQGDMVHPYLRRRCGGEKVEYPGEEVRAVLQRTLGVPIFQEQV
                     MQIAIVAAGFSPGEADALRRAMGAWKRTGGLDPFRERLLTGMHAKGYSQEFAQRIYQQ
                     MLGFGEFGFPESHSLSFALLVYDSAWLKCHEPAAFTCALLNSQPMGFYAPAQLVRDAR
                     AHGVEVRAVDACVSVWDATLERRVDGEPALRLGLRLVKSLSQAGAERLIAARTARPFA
                     GIEDLAARAALDRGDLEALAAAGALSALSGNRHLAFWEVAGTERPLPLAPTAALPASA
                     LEGRPLLAAPTEGQAIAADYASLGLTLGRHPLALLRARLRAAALIPAAELIAAGHGAH
                     VRTAGIVLMRQRPATASGVTFLTLEDESGQVNVIVWERVGREQRRALIESRLLEVHGE
                     LQHQEGVTHLIAQRLIDRSRLLGELTTRSRDFH"
BASE COUNT          543 a         1051 c         1043 g          467 t
ORIGIN      
        1 aggcgcgtga cattgcagtc ctgccgagtc tgatttaagt tcgcgccgtc caattcctgc
       61 agactgccgt cccgcatgag cgagaagccg gaatcagacg agttcgagga cgaagaggcc
      121 gacgagcctg tcatcgagga cggggacgtc gatctcgatc aggtcatcaa ggacctcgat
      181 ctggccaagc gccgtgggca aaaggcgggc gaccccgcgt ggcggcgcct cgagcggctg
      241 ctcgaggaaa aacacaccgc cgagctcacc agcgacttcg aggattacga gatcggcgat
      301 gagggcgagc cgccgggacg caaggcgcgc cgcaagccgc gctcgtagcc tcgcccgact
      361 ccactgcgtg ccacctcgcc cgcggcgttg ccggccgccg cgccgctgct gcgaatcagc
      421 tccgcaaagg ccgtcagtga aaatcgcgcg accgggtagt gagctcaccg agcagccgtg
      481 agcggtcgat gagacgctgg gcgatgaggt gcgtgacgcc ctcctggtgc tgcagctccc
      541 catgcacctc gagcaggcgc gattcgatga gcgcgcgccg ttgctcgcgc ccgacacgct
      601 cccagacaat gacattcacc tgtccgctct catcctcgag ggtgagaaaa gtgacgccgc
      661 tggcggtcgc gggacgctgt cgcatgagca cgataccggc agtgcgcacg tgcgcgccat
      721 gacccgctgc gatcaactcg gctgccggaa tgagcgctgc ggctctcagc cgcgcgcgca
      781 gcagagcgag tggatgtctt ccgagcgtga gtccgagcga ggcatagtcc gcggcgatcg
      841 cctggccttc ggtcggtgca gccagcaatg gccgcccttc cagcgcgctc gccgggagtg
      901 cggcggtcgg tgcgagcggc aaggggcgct ccgtaccggc cacctcccag aaggcgaggt
      961 ggcggttgcc gctcagggcg gagagcgcac cggccgcggc gagcgcctcg agatcgccgc
     1021 gatcgagtgc cgcacgcgcc gcgagatcct cgatgccggc aaagggccgc gcagtacgcg
     1081 cggcgatcag gcgctccgcc ccggcctgcg acagtgactt caccaggcgc aggccgagcc
     1141 gcagtgcggg ctcaccgtcc acgcgccgct cgagcgtcgc atcccagacg ctgacgcacg
     1201 catccacggc acgcacctcg acaccgtgtg cgcgcgcatc acgcaccagc tgcgcgggag
     1261 catagaagcc catcggctgg ctgttgagca gcgcacaggt gaaggcggcg ggctcgtgac
     1321 acttgagcca ggcggagtcg tacaccagca gcgcgaagct gaggctgtga gactcgggaa
     1381 atccgaactc accgaaaccg agcatctgct gatagatgcg ctgtgcgaac tcctgcgaat
     1441 agcccttggc gtgcataccg gtgagcagcc gctcgcgaaa ggggtcgagc ccgccggtcc
     1501 tcttccaggc acccatcgcg cggcgcaacg catccgcctc tcccggcgag aagccggcag
     1561 cgacgatggc aatctgcatg acctgctcct ggaagatcgg tacgccgagc gtgcgctgta
     1621 gcaccgcgcg cacttcttcc ccgggatatt ccactttctc tccaccgcag cggcggcgca
     1681 ggtagggatg caccatgtcg ccctggatgg gtcccggacg cacgatcgcg acctcgatca
     1741 ccagatcgta gtaacagcgc ggcttgaggc gcggcagcat ggccatctgc gcgcgcgact
     1801 cgatctggaa cactccgatg gtgtccgcgc ggcacaccat gtcataaacc ttcggatcct
     1861 cggcgggcaa ctcaccgagc gtataacgcg tgccgcgata atcgttcacc agcgcaaagg
     1921 cacgcttcag tgccgtcagc atcccgagcg ccagcagatc gactttcagc aagccgagat
     1981 cgttgaggtc gtccttgtcc cactgcacca cggtgcgctc tggcatggca gcgttctcga
     2041 tgggcaccag ctcctgcagc gcgccctgcg cgatcacgaa gccgccgacg tgctgcgaca
     2101 gatgcctggg aaagcccgga aaggcggtga gctcgcaggc gagcgacagc agccgtcgca
     2161 gccaggggct gtcggcatgg aagccggcct cgcgcactcg cgcgggaatg gtggcgctgc
     2221 cgtcccacca ttgcatgacc ttggcgagac ggccactctg ctcgggcgtg aggccgaaga
     2281 ccttgccgag atcgcgcaac gcactgcgtg ggcggtacat gatgacggtc gcggcgagcg
     2341 ccgcgcgctc gcgcccatac ttgttgtaga cgtactgcag cacctcctcg cgtcgctcgt
     2401 gctcgaagtc gatgtcgatg tcgggcggtt cgttgcgctc gcgcgagatg aaacgctcga
     2461 acagcagcga tgctccccgc tgcggatcca ctgaggtcac acccaggcaa tagcagacgc
     2521 gtgagttggc cgccgagcca cgtccctggc agaggattcc ctgacggcgc gcgtaagcga
     2581 cgatgtcctg gacggtgagg aaataagact catagtgcag ctcttcgatc aacgccagct
     2641 cgtgctcgat cgcggtgcgc tcggctgccg gcacaccgtc gggccagcgc cagcgggctc
     2701 ccgcttgcgt gaggcgccgc aggtagctcg tcggagtctc tccgggcggc accagttcgc
     2761 gtgggtactc gtagcgcagc tcatcgagtg agaagtggca gctctgcgcc agctcgatgg
     2821 tggccgcgag cagctcgcgc gggtacagcc gcccgaggcg ctcgcgctcg cgcagataac
     2881 gctcgccatt ggcgtagagc cgcgtgccga gctgcgcgac cggctcgttc aggcgaatgg
     2941 cggtaagtgc atcctgcagg cggcggcggc cgcgcacgtg catgtgcaca tcgccgctgg
     3001 cgatgagtcc gatgccgcac tcggcgccga tgcctgccag cgtagcgagg cgccggccgt
     3061 gcgcacctgt gcgcagcagc tccaccgcga tgcgcacgcg atcc
//
LOCUS       DATDMP010000626         3018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1765336, whole genome shotgun sequence.
ACCESSION   DATDMP010000626 DATDMP010000000
VERSION     DATDMP010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765336"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1527)
                     /locus_tag="VI195_11010"
     CDS             complement(223..1527)
                     /locus_tag="VI195_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6824962.1"
                     /translation="MTDSRSDSITFASLGLKPEVMEGIRQAGFVTCTPIQAQTLPIAL
                     AGRDVAGQAQTGTGKTAAFLVALYQALLTRPAPPGRAATSIRALVVAPTRELAVQIHH
                     DALVLGAHTGLRHTVVFGGIDYEKQRQQLSAGCEVLIGTPGRLIDYFKQHVFDLKSAQ
                     VLVLDEADRMFDLGFIADIRYILRRLPPPGRRQSMLFSATLSHRVLELAYEHMNNPEL
                     VRIEPDKMTVDRVRQLMYYPSMEEKVPLLIGLLHQSEAVRTMVFVNTKRMAERLESIL
                     RTNGFQAQALSGDVPQNKRLRFLRDFHEGKLAVLIATDVASRGLHIPDVSHVFNFDLP
                     QDAADYVHRIGRTARAGAEGDAISFACEDTAVSLPDIESYIGHKIPFAPISPELLATS
                     VLTAPHAHRPHAGGGRRQGGGGRRGRRGSHPESAHAHRGRRR"
     gene            1803..2132
                     /gene="trxA"
                     /locus_tag="VI195_11015"
     CDS             1803..2132
                     /gene="trxA"
                     /locus_tag="VI195_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY6824963.1"
                     /translation="MSSPHIVHTTDATFAQDVLKSEKPVLLDFWAEWCGPCKMIAPIL
                     DEIASEYQDRIKVAKINIDENPQTPPKFGIRGIPTLILFKNGTVEAQKVGAVSKSQLS
                     AFLDSNL"
     gene            2315..>3018
                     /gene="rho"
                     /locus_tag="VI195_11020"
     CDS             2315..>3018
                     /gene="rho"
                     /locus_tag="VI195_11020"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY6824964.1"
                     /translation="MNLTELKSKPIHELVKMAETMGLESLARSRKQDIIFSILKAHAR
                     NGENIYGDGVLEILQDGFGFLRSADGSYLAGPDDIYVSPSQIRRFNLRTGDTISGLIR
                     PPKDGERYFALLKVGEINFDSPESSRNKVLFENLTPFHPTKRLKLERGNGSTEDLTAR
                     TIDLVAPIGKGQRGLIVSPPKAGKTMLLQNIATAITHNHPEVYLIVLLIDERPEEVTE
                     MQRTVKGEVVSSTFDEP"
BASE COUNT          565 a          967 c          971 g          515 t
ORIGIN      
        1 tctccacgtt gcggaacagc tcgcgatcga cgttgtcttc gatttccgcc atttcgggca
       61 tgccccagtg gggctgttgg cggcgggcgc cagggtaaca gagcttagcc gagccgcgcc
      121 tcgagtgcca cgcccttccc ccggggtttc cgaaaaatca cgcgtctgtc cgcagggcgc
      181 aaacctcgat acgagcgtca gacgcgattt tctggggtgc ccttaacgac gccgtccgcg
      241 gtgcgcgtgg gcgctctccg ggtgtgatcc gcgccgcccg cgccgtccgc cgccgccctg
      301 acgccgaccc ccacccgcgt ggggccggtg ggcatggggt gcagtgagga cgctggtcgc
      361 cagcagctcc ggcgagatcg gggcgaaggg gatcttgtgg ccgatgtaac tctcgatgtc
      421 gggcagcgag acggccgtgt cctcgcaggc gaagctgatg gcgtcgccct cggccccggc
      481 gcgcgccgtg cggccgatgc ggtgcacata gtccgccgca tcctgcggca aatcgaagtt
      541 gaatacgtgg ctgacatcgg ggatgtgcag cccgcgcgag gcgacatcgg tagcgatgag
      601 taccgcgagc ttgccctcgt ggaagtcccg caggaaacgc aggcgcttgt tctggggcac
      661 atcgccggag agcgcctggg cctgaaagcc gttggtccgc aggatcgact cgagccgctc
      721 ggccatgcgc ttggtattga cgaacaccat ggtgcgcacc gcctcgctct ggtgcagcag
      781 gccgatgagc agcggcacct tctcctccat cgaggggtag tacatgagct ggcgcacgcg
      841 atcgaccgtc atcttgtcgg gctcgatgcg caccagctcg ggattgttca tgtgctcgta
      901 ggcgagctcg agcacccggt gcgagagggt ggccgagaac agcatcgatt gccgccgccc
      961 cggcggcggc aggcggcgca ggatataacg gatatcggcg atgaagccga gatcaaacat
     1021 acggtcggct tcatcgagca ccagcacctg cgctgatttg agatcgaaga cgtgctgctt
     1081 gaaataatcg atcagccgcc cgggcgtgcc gatcagcacc tcgcagcccg cgctgagctg
     1141 ctggcgctgc ttctcgtagt cgatgccgcc gaacaccacg gtgtgtctga gcccggtgtg
     1201 ggctccgagc accagcgcgt cgtgatgaat ctgcaccgca agctcgcggg tcggcgccac
     1261 cacgagcgcc cggatggagg tggcggcgcg gcccggcggc gccggccgtg tgagcagcgc
     1321 ctgatagagc gcgacgagga acgcggccgt cttgccggtg ccagtctgcg cctgcccggc
     1381 aacatcacgc ccggcgagcg cgatcggcag cgtctgtgcc tgtatcggag tgcaggttac
     1441 gaagccggcc tggcgtatgc cctccatgac ctcaggctta aggccgagcg aggcgaaggt
     1501 gatggagtcg gagcgtgaat cggtcatcag ttagagcgag tggttagggg caaattgccc
     1561 ctacagactt gaaaaatgcg cccggaagcg ctacaaagtg tgtgaagaca gcgggccggc
     1621 aggtccgcac ctgccgtacc cacccttagt tccttttgga tacatactca atcacgagtt
     1681 attagtgtaa ccgtttaccg ggagtctctg gcatcgcttt gcgcggcttc ctggccgacc
     1741 ccgcggcgga ttcactttcc aagggatttt tcacgctctg tatttgacgg aggttgaacc
     1801 gcgtgagtag cccgcacatt gtgcacacga cggacgccac attcgcacag gacgtcctga
     1861 agtccgaaaa gccggtgctg ctggacttct gggccgagtg gtgcggtccg tgcaagatga
     1921 tcgctccgat cctcgatgag atcgccagcg agtaccagga ccgcatcaag gtcgccaaga
     1981 tcaacatcga tgaaaacccg caaaccccgc cgaaattcgg catccgcggc atcccgacgc
     2041 tgattctgtt caagaacggc acggtcgagg cgcagaaggt gggtgcggtc tccaaatccc
     2101 aactttccgc atttctggac agcaacctgt gagaggctat ctcggtaatc agggccgtaa
     2161 ccggcccaac aagggcccgc gccacggcaa ccgtgacggc aaccgcggca acggcggcgg
     2221 ccggcccgat gagatgatgc acgagccgga ggtgttcgac gccgacgact ccgagatcat
     2281 cagtcccgcc gagctgacgc agtcgcgcag ctccatgaac ctgacggagc tgaagtccaa
     2341 gcccatccac gagctggtga agatggccga gaccatgggg ctcgagagcc tcgcccgctc
     2401 gcgcaagcag gacatcatct tctcgatcct caaggcgcac gcgcgcaacg gcgagaacat
     2461 ctacggcgac ggcgtgctcg agatcctgca ggatggcttc ggcttcctgc gctcggcgga
     2521 tggttcctac ctcgccggac ccgacgatat ctatgtgagc ccgagccaga tccggcgctt
     2581 caacctgcgc accggcgaca ccatctccgg gctgatccgg ccaccgaagg acggtgagcg
     2641 ctatttcgcg ctgctgaagg tgggcgagat caacttcgac tcgcccgaga gctcgcgcaa
     2701 caaggtgctg ttcgagaacc tgacgccgtt ccacccgacc aagcgcctga aactcgaacg
     2761 cggcaacggc tccaccgagg accttaccgc gcgcaccatc gacctggtcg ccccgatcgg
     2821 caagggtcag cgtggcctga tcgtctcccc gcccaaggcc ggcaagacga tgctgctgca
     2881 gaacatcgcc actgccatca cccacaacca tcccgaggtc tacttgatcg tgctgctgat
     2941 cgatgagcgg cccgaggaag tcacggagat gcagcgcacg gtcaagggtg aggtggtgtc
     3001 ctccaccttc gatgagcc
//
LOCUS       DATDMP010000627         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1432686, whole genome shotgun sequence.
ACCESSION   DATDMP010000627 DATDMP010000000
VERSION     DATDMP010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432686"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="phoR"
                     /locus_tag="VI195_11025"
     CDS             <1..954
                     /gene="phoR"
                     /locus_tag="VI195_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY6824965.1"
                     /translation="EWCNDTASAQLGLDPDADSGQAIANLVRAPSFIDYLAGIDGAPI
                     RVPIRENLLALQLVPYGQAQKLLLSRDVTQAERVETLRRDFVANVSHELRTPLTVLVG
                     FLETVRELKLDPQRTRDYLGMMQEQSARMQRIIEDLLTLSLLESAPSPPAERVRLRPL
                     LERVLADAEALSAGRHRVTLNAWPTVDLLGSESELASAFGNLATNAVRYTPRGGEVRL
                     VWHDGPDGSSFSVEDTGVGIAAEHLPRLTERFYRVDRSRSRETGGTGLGLAIVKHAAA
                     RHQAKLEIDSHPGKGSRFTLRFPGKRTVPAEPAGERAPASG"
     gene            1501..>2459
                     /locus_tag="VI195_11030"
     CDS             1501..>2459
                     /locus_tag="VI195_11030"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEY6824966.1"
                     /translation="MNTYARQPVAFVRGEGVWLWDEDGKKYLDALAGIAVNTLGYAHP
                     KLVAALTKRIQSGVIHTSNLWRIPDQEMAADRVAEITGLDEVFFCNSGLEANECAIKI
                     ARKYGHDRGVAEPAIVVMEKAFHGRSLATLSATGSRKVQAGFEPLVTGFVRVPLNDLE
                     AVRQAAEHNRNIVAVMVEPIQGEGGINVSRMEYLRGLYELCQRKQWLFISDEVQCGLG
                     RTGKWFVYQHAGFLPDVVPLAKGLGSGVPVGACVIGGRAKGVLKPGNHGSTFGGNPLA
                     MTAVRTTIDTIKEEGLLANAERVGALLQHSLKESLGQVKGVAE"
BASE COUNT          429 a          800 c          814 g          416 t
ORIGIN      
        1 gagtggtgca acgacacggc ttccgcgcag ctgggccttg atccggacgc cgattccggc
       61 caggcgatcg ccaacctggt gcgcgcgccg tcgttcatcg attacctggc gggcatcgac
      121 ggcgcgccga tccgcgtgcc gatccgggag aacctgctcg cgctccagct cgtcccgtac
      181 gggcaggcgc agaagctcct gctgtcgcgc gacgtgacgc aggcggagcg cgtggagacg
      241 ctgcggcgtg atttcgtggc caacgtgtcg cacgagctgc gcaccccgct gaccgtgctg
      301 gtgggcttcc tggagaccgt gcgcgagctg aagctcgatc cccagcggac gcgcgactac
      361 ctcgggatga tgcaggagca gtcggcgcgc atgcagcgca tcatcgagga tctcctcacg
      421 ctttcgctgc tggaatcggc gccctcgcct ccggccgagc gcgtgcggct caggccgctt
      481 ctcgagcgcg tgctcgccga cgccgaggcg ctctccgccg gccggcatcg cgtcacgctg
      541 aacgcctggc ccacggtcga cctgctcgga tcggaaagcg agctggcgag cgcgttcggc
      601 aacctcgcga ccaacgccgt ccgctatacg ccgcgcggcg gagaggtacg cctagtctgg
      661 cacgacggtc cggatggctc ctctttcagc gtggaggaca ccggcgtcgg catcgcggcc
      721 gagcacctgc cgagactcac cgagcgtttc tatcgcgtgg atcggagccg gtcgcgcgag
      781 acggggggaa ccgggttggg cctggcgatc gtgaagcacg ctgcagcgcg gcatcaggcg
      841 aagctggaga tcgacagcca cccgggaaag ggaagccgat tcacgctgcg ttttccgggg
      901 aagcgcacgg ttccggcgga accggcggga gagagagcac ctgcctccgg gtaactgctc
      961 ccttgtggag gcaggcgccc tttacggcat tacttcttct ttttggacga cttctttgct
     1021 tttttgcgtt ttgccacttt tggtctccct gagggtccca tcaccggaaa aacccgatga
     1081 ttagactcgt gtgtacttga acgcgcatgt tgatgcaact aattgagccg gctaagtacg
     1141 cttgcgcgcg gatgtttgtg gcaaaaaaaa cacactcatc ggcgaagcgc gctcaatgct
     1201 ccatttattt gcgatgcacc tgatgcaatc gtgaaccgct gccgctggaa tccaagtgcg
     1261 ccactgcatc gcaacagaag cgatgctttc aaagagacgg aacgaattgt ccggccgcgt
     1321 tcattcgatc tgcgcacagc ctcaccgagc tcgcgacgtg aagcaccgat cccctgatgg
     1381 cgctcgtcgt tcggctcgac cgcgggacgc tatccaagag ccttaacccc tgatagcatt
     1441 gaagaaacgg cggcatcgag ccgccgtttt gcatttggga gagcgcgcat gtcgcacgtc
     1501 atgaatacct acgcccggca gccggtggcc ttcgttcgcg gcgaaggcgt gtggctctgg
     1561 gacgaagacg gcaagaagta cctcgacgcg ctcgccggca tagcggtgaa cacgctcggc
     1621 tacgcgcatc cgaagctggt cgcggccctg acgaagcgaa ttcaaagcgg cgtcatccat
     1681 acgtccaacc tgtggcgcat cccggaccag gagatggccg ccgaccgcgt ggccgagatc
     1741 accggcctcg acgaggtctt cttctgcaac tcgggcctcg aggccaacga gtgcgcgatc
     1801 aagatcgcgc gcaagtacgg gcacgaccgg ggcgtcgccg agcccgcgat cgtcgtgatg
     1861 gagaaggcgt tccacggccg ctcgctcgcg acgctctccg ccaccggcag ccgcaaggtg
     1921 caggcgggct tcgagccgct cgtcacgggc ttcgtgcgcg tgccgctcaa cgacctcgag
     1981 gccgtgcgcc aggccgccga gcacaaccgc aacatcgtcg cggtgatggt cgagccgatc
     2041 cagggcgaag gcggcatcaa cgtctcgcgc atggaatacc tgcgcgggct ctacgagctt
     2101 tgccagcgca agcagtggct gttcatctcg gatgaggtgc agtgcggtct cggccgcacc
     2161 ggcaagtggt tcgtctacca gcacgccggc ttcctgccgg acgtcgtgcc gctcgccaag
     2221 gggctgggct ccggcgtgcc cgtgggcgcc tgcgtgatcg gcggccgcgc caagggcgtg
     2281 ctgaagccgg gcaatcacgg ctcgaccttc ggcggcaacc cgctcgcgat gaccgcggtg
     2341 cgcacgacga tcgacacgat caaggaagaa ggcctgcttg ccaacgccga gcgcgtcggc
     2401 gcgctgctgc aacacagcct gaaggagagc ctgggccagg tgaaaggcgt cgcggagat
//
LOCUS       DATDMP010000628         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1876437, whole genome shotgun sequence.
ACCESSION   DATDMP010000628 DATDMP010000000
VERSION     DATDMP010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876437"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="pqqE"
                     /locus_tag="VI195_11035"
     CDS             complement(<1..1006)
                     /gene="pqqE"
                     /locus_tag="VI195_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY6824967.1"
                     /translation="MSGPPSPPLWLLAELTYRCPLHCVYCSNPVDYTRYGEELATEDW
                     RRVLREARELGAAQLGFSGGEPLMRDDLEALAAEAHRLGYYSNLITSGVGLNERRIAA
                     LRQAGLDHIQVSFQDSTREMNDFLSSTRTFELKRRVARLVKQHGYPMVLNVVLHRLNI
                     DHVAQILDMAEELEADDVELANTQYYGFAYANRAQLLPSREQLERAEEATRRFRDRVG
                     ERMKIYFVVPDYYANRPKACMNGWGAVFLCVTADGVALPCHEARMLPGLEFPSVRSHS
                     LRSIWNDSPAFNRYRGEDWMREPCRSCERRSVDFGGCRCQAYQLAGDARAADPVCKLA
                     P"
     gene            complement(990..1262)
                     /gene="pqqD"
                     /locus_tag="VI195_11040"
     CDS             complement(990..1262)
                     /gene="pqqD"
                     /locus_tag="VI195_11040"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY6824968.1"
                     /translation="MTDDCVLELARHYRFQWEPAQQAYVLLYPEGMVKLGPSAAEILK
                     RIDGQKAVAAIVADLERAYPGADLRGDVAEFLAHAQQKGWLRVRPA"
     gene            complement(1255..1959)
                     /gene="pqqC"
                     /locus_tag="VI195_11045"
     CDS             complement(1255..1959)
                     /gene="pqqC"
                     /locus_tag="VI195_11045"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY6824969.1"
                     /translation="MRDALPWSQREFEAQLRAKGARYHIHHPYHVAMNSGRCTREQVQ
                     GWVLNRFYYQVCIPVKDAAILANMPDREERRQWIQRILDHDGTQGNEGGIEAWLRLGE
                     AVGLARQELLSLQHVLPGVRFAVDAYVDFARRAPWPEAVCASLTELFAPQIHEQRLAG
                     WPQHYPWIEPSGYAYFRGRLAEARRDVEHGLRVTLEHFRTRAEQERALVILQFKLDVL
                     WSMLDAMQLAYGIGHD"
     gene            complement(1959..>2525)
                     /locus_tag="VI195_11050"
     CDS             complement(1959..>2525)
                     /locus_tag="VI195_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6824970.1"
                     /translation="DLTQGHPVFGVLEHFCGVDWHPIAADARTAFSVAGAQGLSFTAV
                     PLKSKPPPYSPHRNEPRPGDNIGLRVVDLRTRRSLFYAPGLGEIEPHLRPFLAEADCL
                     MLDGTFWTEDELIQLGVSAKRAADMGHLPQSGPGGMVEVLAKLAAPRKVLIHINNTNP
                     ILDEDSPERAALRTAGIEVAFDGMEIEL"
BASE COUNT          397 a          852 c          877 g          399 t
ORIGIN      
        1 ccggcgcgag cttgcacacc gggtcggcgg cgcgcgcatc gccggcgagc tggtacgcct
       61 ggcagcgaca gccgccgaag tcgacgctgc ggcgctcgca gctgcggcag ggctcgcgca
      121 tccagtcctc gccgcgatag cggttgaagg cgggcgagtc gttccagatg gagcgcaggc
      181 tgtggctgcg cacgctcggg aattccagcc cgggcagcat gcgcgcctcg tggcacggca
      241 gcgcgacccc gtcggcggtg acgcacagaa ataccgcgcc ccagccgttc atgcacgcct
      301 tcggccgatt cgcatagtag tccgggacga cgaagtagat cttcatccgc tcgcccacgc
      361 ggtcgcggaa gcggcgcgtc gcctcttcgg cgcgctcgag ctgctcgcgg ctgggcagga
      421 gctgcgcgcg gttggcgtag gcaaagccgt agtactgcgt gttcgcgagc tccacgtcgt
      481 cggcttccag ctcctccgcc atgtcgagga tctgcgccac gtgatcgatg ttcaggcgat
      541 gcagcacgac gttgagcacc atcggatagc cgtgctgctt caccagccgg gcgacgcggc
      601 gcttcagctc gaaggtacgg gtgctcgaca ggaagtcgtt catctcgcgc gtcgagtcct
      661 ggaaggaaac ctggatgtgg tccagcccgg cctggcgcag cgcggcgatg cgccgctcgt
      721 tcagcccgac gcccgaggtg atcaggttcg agtagtagcc gagccgatgc gcttcggcag
      781 cgagcgcttc caggtcatcg cgcatcagcg gctcgccgcc ggagaagccg agctgcgcgg
      841 cgccgagctc gcgcgcctcg cgcagcacac gccgccagtc ctccgtggcg agctcctcgc
      901 cgtagcgcgt gtaatccacc gggttcgagc aatacacgca gtgcagcgga caacgatagg
      961 tcagctccgc cagcagccag agcggcgggc taggcgggcc ggacacgcag ccaccctttt
     1021 tgctgcgcat gcgccaggaa ctcggcgaca tcgccgcgca ggtcggcacc ggggtaggcg
     1081 cgctcgaggt cagcgacgat cgctgcgacg gctttctggc cgtcgatgcg cttgaggatc
     1141 tcggcggcgc tcggccccag cttcaccatt ccctcgggat agagcagcac ataagcctgc
     1201 tgcgccggct cccattggaa tcggtaatgg cgcgcgagct ccaggacgca gtcgtcagtc
     1261 atggcctatc ccataggcga gctgcatcgc gtccagcatc gaccagagca catcgagctt
     1321 gaactgcagg atgacgagcg cccgctcctg ctcggcgcgg gtgcggaaat gctccagcgt
     1381 cacgcgcagg ccgtgctcga cgtcgcgcct tgcttcggcg aggcggcccc ggaaataggc
     1441 gtagccggaa ggctcgatcc agggatagtg ctgcggccag ccggcgagcc gctgctcgtg
     1501 gatctgcggc gcgaacagct cggtgagcga ggcgcaaacg gcttccggcc acggggcacg
     1561 gcgcgcgaag tcgacatagg cgtctaccgc gaagcgcacc ccgggcagca cgtgctgcag
     1621 cgagagcagc tcctggcgcg cgagtcctac cgcttcgccg aggcgcagcc aggcctcgat
     1681 gccgccttcg ttgccctgcg tgccgtcgtg gtcgaggatg cgctggatcc attgccggcg
     1741 ctcttcgcgg tccggcatgt tggcgaggat ggccgcgtcc ttcacgggaa tgcacacctg
     1801 gtagtagaag cggttcagca cccagccctg cacctgctcg cgcgtacagc ggccgctgtt
     1861 catcgcgacg tgatacggat ggtgaatgtg gtagcgggcg cctttggctc gcagctgcgc
     1921 ctcgaactcc cgctggctcc atggcaaagc atcgcgcatc acagctctat ctccatgccg
     1981 tcgaaagcga cctcgatgcc ggcggtgcgc agcgccgcgc gctcgggcga gtcctcatcg
     2041 aggatcgggt tggtgttgtt gatgtggatg agcaccttgc gcggcgcggc gagcttcgcc
     2101 agcacttcga ccatgccgcc cgggccggac tgcggcaggt gccccatgtc ggcggcgcgc
     2161 ttggccgaca cgccgagctg gatcagctcg tcctcggtcc agaaggtgcc atcgagcatc
     2221 aggcagtccg cctccgcgag gaacggccgc aggtgcggct cgatctcgcc cagccccggc
     2281 gcgtagaaga gcgaacggcg ggtgcgcagg tcgacgacac gcaggccgat gttgtccccc
     2341 ggccggggct cgttgcggtg cggcgagtac ggcggcggct tgctcttcag cggcacggcg
     2401 gtgaacgaaa gcccttgcgc tcccgctacc gaaaaggcgg tccgggcatc ggctgcgatg
     2461 ggatgccagt ccacgccgca gaaatgctcg agcacgccga acaccggatg gccttgcgtc
     2521 aggtc
//
LOCUS       DATDMP010000629         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1542725, whole genome shotgun sequence.
ACCESSION   DATDMP010000629 DATDMP010000000
VERSION     DATDMP010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542725"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VI195_11055"
     CDS             <1..894
                     /locus_tag="VI195_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY6824971.1"
                     /translation="GTAVVIDDEWGKPVDPQKVEDAFKKNPDAKVLAFVHAETSTGAQ
                     SDARVLTQIAHKHGAMVIVDAVTSLGGTPVKVDEWEIDAIYSASQKCLSCTPGLSPVS
                     FNERVVEHVKKRKDKIHSWFMDMNLLFNYWSAGTRTYHHTAPTNSLFALHEALLLIRE
                     EGLENAWARATRHHLALKAGLEAMGMKMLVAEKYQLPQMNAVICPQGVNEAEVRKRLL
                     NEFGIEIGAGLGPLAGKIWRIGLMGYSCRPDNVMLCLSALGSVLSDMGLPVHVGDAEA
                     AAHGAYAQMHAKDAQRKKRAA"
     gene            complement(891..1733)
                     /gene="metF"
                     /locus_tag="VI195_11060"
     CDS             complement(891..1733)
                     /gene="metF"
                     /locus_tag="VI195_11060"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005399750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY6824972.1"
                     /translation="MGNRVLSFEFFPPKTPAGKSRLRETWQQLAALKPDFFSVTFGAG
                     GSTREGTLETALEIRAAGHEAAPHLSCIASSREEVKAQLERYKSHGIRHIVALRGDLP
                     SGIASAGEFRYATELVEFIRRSTGDWFQIEVACYPEYHPQTRRAQDELRHFRSKVQAG
                     ANSAITQYFYNADAYFRFVDEARTAGISVPIVPGVMPIVNFAQLARFSDACGAEIPRW
                     LRLKLESFHDDSASIRALGVDVVSALCERLLAGGAPGLHFYTLNQAALTLEICGRLQL
                     VRQP"
     gene            complement(1723..2637)
                     /locus_tag="VI195_11065"
     CDS             complement(1723..2637)
                     /locus_tag="VI195_11065"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6824973.1"
                     /translation="MKIAAIGTGGVGGYFGARLQQAGEDVAFLARGKHLAAIRANGLK
                     LESPLGSARLKVKPYERPEEIGPVDVVMFAVKLWDTEAAAQSIKPLLAGGGVVIPFQN
                     GVESIETLRKILGQRPVMGGAAYIASRISAPGVIEHIGQMQRLQFGPLLPEQRPVAER
                     LLAACKKAGINAEISDDVVRSIWEKFAFLVAVSSATAVSRRPLGVVRTDPDLRRLFEQ
                     AMRETWTLARARGIELADDFIETRMKHLDSLPAEMKASMAHDLEAGGKLEAPWLCGAV
                     ARMAREAGIAAPVNETVFAALKPYLNGK"
     gene            complement(2645..>3360)
                     /locus_tag="VI195_11070"
     CDS             complement(2645..>3360)
                     /locus_tag="VI195_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY6824974.1"
                     /translation="GCRESLVDGIKRATDVMVAGKVAVVCGYGEVGKGSAQALRGLQA
                     QVWVTEIDPICALQAAMEGYKVVTMEYAADKADIFVTATGNFHVITHAHMAKMKNNAI
                     VCNIGHFDNEIDIASVKKYKWENIKPQVDHIIFPDGKRIILLAEGRLVNLGCATGHPS
                     YVMSSSFANQVLAQIELYGNNAKYPVGVYILPKHLDEKVARLQLEKLGAMLTEMTDEQ
                     AKYVGLPKQGPYKSDHYRY"
BASE COUNT          551 a         1106 c         1125 g          578 t
ORIGIN      
        1 ggcacggcgg tggtcatcga cgacgagtgg ggcaagccgg tcgatccgca gaaggtcgag
       61 gacgcgttca agaagaaccc ggatgccaag gtgcttgcct tcgtgcatgc ggagacttcc
      121 accggcgcgc agtccgacgc gagggttctc acccagatcg cgcacaagca cggcgcgatg
      181 gtgatcgtcg atgcggtgac ctcgctcggc gggacgccgg tgaaggtgga cgagtgggag
      241 atcgacgcga tttactcggc gagccagaag tgcctctcgt gcacgcccgg cctctcgccg
      301 gtatcgttca acgagcgcgt ggtcgagcac gtgaagaaga ggaaggacaa gatccacagc
      361 tggttcatgg acatgaacct gctcttcaac tattggagcg cgggcacgcg cacctatcat
      421 cacaccgcgc cgaccaactc gctctttgcg ctgcacgagg cgctgctcct catccgcgag
      481 gaggggctcg agaatgcgtg ggcgcgcgct acgcgccacc atctcgcgct caaggcgggc
      541 ctggaggcga tgggcatgaa gatgctggtg gcggagaagt accagctgcc gcagatgaac
      601 gcggtgatct gcccgcaggg cgtgaacgag gccgaggtga ggaagcgcct gctcaacgaa
      661 tttggcatcg agatcggcgc cggcctcggg ccgctcgccg gcaagatctg gcgcatcggc
      721 ctgatgggct acagctgccg cccggacaac gtgatgctct gcctttcggc gctgggctcg
      781 gtgctctccg acatgggcct gcccgtgcac gtcggcgatg cggaagcggc ggcgcacggc
      841 gcctacgcgc agatgcacgc caaggacgcg cagagaaaga agcgcgccgc ctagggttgc
      901 cggaccagct gcagccggcc gcagatctcc agcgtcaggg ccgcctggtt cagcgtgtag
      961 aagtgaaggc ccggcgcgcc gccggcaagc aggcgttcgc acagcgcgct caccacgtcg
     1021 acgccgagcg cgcggatcga agcgctgtcg tcgtggaagc tctcgagctt caggcgcagc
     1081 cagcgcggga tctcggcgcc gcacgcgtct gaaaaccggg caagctgcgc gaaattcacg
     1141 atgggcatga ccccgggcac gatcggcacc gagatgcccg cggtgcgcgc ctcgtccacg
     1201 aaccggaaat acgcatccgc gttgtagaaa tactgggtga tcgcggaatt cgcgcccgct
     1261 tgcaccttgc tcctgaagtg gcgcagctcg tcctgcgcgc ggcgcgtctg cgggtggtat
     1321 tccgggtagc acgccacctc gatctggaac cagtcccccg tcgagcgccg gatgaactcc
     1381 acgagctcgg tggcgtagcg gaattccccg gcggaggcga tgcctgaggg caggtcgccg
     1441 cgcagcgcca cgatgtgccg gatgccgtgg gatttatagc gctcgagctg cgccttcact
     1501 tcctcgcgcg aagaggcgat gcacgaaaga tgcggggcgg cttcgtgccc ggcagcgcgg
     1561 atctcgagag cggtttccag cgtgccttcg cgggtcgagc cgccggcgcc gaaggtcacc
     1621 gagaagaaat cgggcttgag ggccgcgagc tgctgccagg tctcgcgcag cctggatttc
     1681 cccgccggcg tcttcggtgg aaagaactcg aaacttaaga ccctatttcc cattcaggta
     1741 gggcttgagc gcggcgaata cggtctcgtt caccggcgcg gcgatgcccg cctcgcgcgc
     1801 catgcgcgct accgcgccgc acagccacgg cgcctcgagc ttgccgcccg cctcgaggtc
     1861 gtgcgccatc gacgccttca tctccgccgg cagcgaatcc aggtgcttca tccgcgtctc
     1921 gatgaagtcg tcggcgagct cgatgccgcg cgcgcgggcg agggtccagg tctcgcgcat
     1981 cgcctgctcg aagagccgcc gcaagtccgg atcggtgcgc acgacgccga gcggccggcg
     2041 cgatacggcg gtagcgctcg acacggcaac caggaaggcg aacttctccc agatgctgcg
     2101 cacgacatcg tccgatatct cggcgtttat tccggctttc ttgcatgccg ccagcaaccg
     2161 ctccgcaacc gggcgctgct ccggaagcag cggcccgaac tgcagccgct gcatctggcc
     2221 gatgtgctcg atgacaccgg gcgccgaaat gcgcgaggcg atgtaggcgg cgccgcccat
     2281 caccgggcgc tggccgagta ttttccgcag cgtctcgatg gactcgacgc cgttctggaa
     2341 cgggatgacc acgccgcctc ctgcgagcag cggcttgatg ctttgcgccg cggcctcggt
     2401 gtcccagagc ttcacggcga acatcaccac gtcgaccggc ccgatctcct cgggcctttc
     2461 gtagggtttc accttcagcc tcgccgagcc gagcgggctc tcgagcttca ggccgttcgc
     2521 gcggatggcg gcgagatgct tgccgcgcgc gaggaaagcg acgtcttctc ccgcctgctg
     2581 gagccgggcg ccgaagtagc cgccgacgcc gccggtcccg atcgctgcga ttttcatcgg
     2641 cgcttcagta gcgataatgg tcggacttgt acgggccttg cttcggcagg ccgacgtact
     2701 tggcctgctc gtccgtcatc tcggtcagca tggcgccgag cttttcgagc tgcaggcgcg
     2761 ccaccttctc gtccaggtgc ttcggcagga tgtagacgcc gaccggatac ttggcgttgt
     2821 tgccgtagag ctcgatctgc gccagcacct ggttcgcgaa cgacgagctc atcacgtacg
     2881 acgggtgccc ggtggcgcag ccgaggttca cgagccggcc ctcggcgagc aggatgatgc
     2941 gcttgccgtc cggaaagatg atgtggtcca cctgcggctt gatgttctcc cacttgtact
     3001 tcttgacgga tgcgatgtcg atctcgttgt cgaagtggcc gatgttgcag acgatggcgt
     3061 tgttcttcat tttcgccatg tgcgcgtgcg tgatgacgtg gaagttgccg gttgcggtca
     3121 cgaagatgtc cgccttgtcc gcggcgtact ccatggtgac gaccttgtag ccttccatcg
     3181 cggcctggag cgcgcagatc gggtcgatct cggtgaccca cacctgcgcc tgcaggccgc
     3241 gcagcgcctg cgccgagccc ttgccgacct cgccatagcc gcacaccaca gcgaccttgc
     3301 ccgccaccat cacgtcggtg gcgcgcttga tgccgtccac cagcgactcg cggcagccgt
//
LOCUS       DATDMP010000630         5167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_210695, whole genome shotgun sequence.
ACCESSION   DATDMP010000630 DATDMP010000000
VERSION     DATDMP010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210695"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..994)
                     /locus_tag="VI195_11075"
     CDS             complement(164..994)
                     /locus_tag="VI195_11075"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HEY6824975.1"
                     /translation="MSARAEADTVAVEVRDSIAWVRFNRPEKRNAMSPQLNRQMLRVI
                     NELEFRDDVGVLVLAGEGAAWSAGMDLKEYFRETEAQGLAGVRHSQREAYAWWERLRW
                     YQKVTIAMVNGWCFGGAYGPLFACDLAFCAEEAQFGLSEINWGILPGGGATKVAVELM
                     PMRSAMYHALLGETLSGREAQAAGLVNEAAPADQRAARVTEVAKRLLEKNWETLKATK
                     DAVRRVREMSYDNAQDYLIRAQEALNWHDKSDGRHRGMKQFLDDKSYKPGLTQYDKHK
                     "
     gene            1069..1518
                     /locus_tag="VI195_11080"
     CDS             1069..1518
                     /locus_tag="VI195_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824976.1"
                     /translation="MKDPLTALCGYLLQRASAAALAELNERLAPLGLRHAGVALLMLV
                     EARPGITQSQAGRVLDIQRANMVPFVARLEGLGVLRRRQVDGRSQALELTGRGRMQLE
                     RARRVVAAHEAQLLERVPEKLRPMVLPVLMALWQGEPEAAAAQRTAR"
     gene            1542..2324
                     /locus_tag="VI195_11085"
     CDS             1542..2324
                     /locus_tag="VI195_11085"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEY6824977.1"
                     /translation="MIAYAGRAVPRPRYYANDHSRDVLDITLTAMDVADGRALSPTLD
                     EAGFTLVSHRSALGDFTDRAAVEATHRPEIVALIGELSGADLVLATSPGILRFSERAP
                     QSGTLDNSRPARFAHVDVSDATAAMFAQRAVPPGRTLARFVHYNIWRALSPPPQDVPL
                     AVCDARSVAAADLIAADAVFDSPGKPEWSFEGLLLAHAAAHRWHWFPDMQRDEALIFK
                     THDSDRGRAHCVPHVAFDHTACPAHAAPRVSIEMRALALWFA"
     gene            2350..3819
                     /locus_tag="VI195_11090"
     CDS             2350..3819
                     /locus_tag="VI195_11090"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY6824978.1"
                     /translation="MHRFPNTPEFTGLNTPIGAEYELAALPVEGRIPEEVAGTFFRAI
                     PDPAFPPYIEDGAAVLSGDGMVSALRFAPGKVSFAVRFVETERHRAEVRAGRALFGKY
                     RNPFTDRPEAAGLDRTVANTTPVWHAGRLLMTKEDGRPYRIDPHTLATLGRYDFGGRL
                     KSATVTAHVRIDPHTGELFFFGYEADGLASTTVSYCIADARGNLTREQWFDAPYCAMM
                     HDFTITERYALFPIYPTTCDLERLRSGGDHWVHELQRDSWVGVMPRYGSVGELRWFRG
                     PKGVSCYHMMNAFEDADGRIHLDQCLANVNAFPFIQRASGLDVAPGDTGARLARWTID
                     LRDGTERVSESTIGPPGDLPVIPAASQGRPYGHGWLLTMNPDMQGPPVAGGPVGVMFN
                     TLLRLDFTGGPPQALALPPGHCLSEPVHVPSSQSGHEGWLLTIVDRQVSESDFAHTLW
                     ILEAGNLAAGPIARVPIAHRLRPQVHGWWVSAGELAAAG"
     gene            complement(3828..4676)
                     /locus_tag="VI195_11095"
     CDS             complement(3828..4676)
                     /locus_tag="VI195_11095"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEY6824979.1"
                     /translation="MTLAGAEVESKIDYLVPSSRINRRFWAPGRELNTGVYAPYPVTI
                     RNARLAGPFTLDAHGFCLARHRSAIGDWENQYAPGSAYAAEVAEVAKSLSGADLVIPL
                     GGMLRDSAVTSPTVQPPAAEAHVDFTERCAERRAAALYRQAYPEASGYRRFIGFSLWR
                     ALSAPPQDMPLALCEGRSVRDEEGTHNTKVDVTEIPTGEALYAPIAGEEDMLAATIFH
                     YSPGHRWWYFPDMEPDEVIFIKLYDSDHSKAWRCPHTAFRDTTRPDARPRRSMEFRAM
                     AYFSNT"
     gene            complement(4684..>5167)
                     /locus_tag="VI195_11100"
     CDS             complement(4684..>5167)
                     /locus_tag="VI195_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6824980.1"
                     /translation="QKQFTQGHNGPRYGWLLDHPQMQMVIDVAPDRRTANVRGRSMMQ
                     AGLHHTAEGQQRAWWEGGIYENTYLRENGVWKIKALRYYPFWHGSFAEGWPKTPIDFV
                     PMAKTLYPQDPLGPDELMNPQPRLWPATDTVAFHYPHPVTGKPIVLDNSRAREGFKAG
                     "
BASE COUNT          769 a         1762 c         1735 g          901 t
ORIGIN      
        1 atggctccgg tgatcgccgg ctacctgttc caggcggggc tgccgctgag ctcggtcgct
       61 ctggtgatgg cgggcggctc gctgatcgcg gcgttggtgc tctcggcaat gtgggtgcgc
      121 gaagcggact gagcttcagg caacgtgccg cgacggcgcc gcgtcatttg tgcttgtcgt
      181 actgggtcag gccgggcttg taggacttgt cgtcgagaaa ctgcttcatg ccgcggtggc
      241 ggccgtccga cttgtcgtgc cagttgagcg cctcctgggc acggatcaga tagtcctgcg
      301 cgttgtcgta gctcatctca cgcacgcgcc gcaccgcgtc cttggtggcc ttgagcgtct
      361 cccagttctt ctcgagcagc cgcttcgcca cctcggtcac gcgtgccgcg cgctggtccg
      421 ctggtgccgc ctcgttgacg aggcccgccg cctgtgcctc cctgccgctg agtgtctcgc
      481 cgagcagggc gtgatacatc gcgctgcgca tcggcatgag ctcgaccgcc accttggtgg
      541 cgccgccgcc ggggagaatc ccccagttga tctccgaaag accgaactgc gcctcctccg
      601 cgcagaaggc gagatcgcag gcaaagagcg gtccataggc gccgccgaag caccagccat
      661 tcaccatcgc gatcgtgacc ttctggtacc agcgcaaccg ctcccaccag gcatacgctt
      721 cgcgctgcga atggcgcaca cccgcgaggc cctgcgcctc ggtctcacgg aagtactcct
      781 tgaggtccat tcccgccgac caggcagcgc cctcgccggc gagcaccagc acgcccacat
      841 cgtcgcggaa ctcgagctcg ttgatgacgc gcagcatctg ccggttgagc tgcgggctca
      901 tggcgttacg cttctccggg cgattgaacc tcacccaggc gatgctgtcc cggacctcga
      961 cggccaccgt atcggcttca gcgcgtgcgc tcatgccctg ccacttcaat tgctatgata
     1021 tatagcagta taatccgggc gggcagcgcc gacaaccgtg cgcgcattat gaaagaccct
     1081 ctgaccgcgc tttgtggcta cctcctgcag cgcgcttcgg cggccgcgct cgcggagctc
     1141 aatgagcggc tcgcgccgct cgggctgcgg cacgcggggg tggccctgct catgttggtc
     1201 gaggcccgtc ccggcatcac ccagagccag gcgggccgcg tgctcgacat ccagcgcgcc
     1261 aacatggtgc cgttcgtcgc ccgcctggag ggtctcgggg tgctgcgtcg tcggcaggtc
     1321 gatggtcgct cacaggcgct cgagctgacc ggccgcgggc gcatgcagct cgagcgggcg
     1381 cggcgcgtgg tggcggcgca tgaggcgcag ctcctcgagc gcgtgcccga gaagctgcgg
     1441 ccgatggtgc tgccggtgct gatggcgctg tggcagggtg aacccgaagc cgctgccgcg
     1501 cagcgcacgg cgcgctgaac atcgacatgg acacgcgcgc catgattgcc tatgcaggac
     1561 gcgcggtgcc gcggccacgc tattacgcca acgatcacag ccgcgatgtt ctcgacatca
     1621 cgctcaccgc catggacgtc gcggacgggc gcgcgctcag cccgacgctc gatgaggccg
     1681 gattcacgct cgtctcgcac cgcagtgcgc tcggcgattt caccgaccgc gccgcagtcg
     1741 aagccacgca ccgcccggag atcgttgcgc tcatcggtga gctctcgggc gccgatctag
     1801 tgctggccac gagccccggc atcctgcgct tcagcgagcg cgcgccgcag tcggggacgc
     1861 tcgacaattc ccggccggcg cgcttcgcgc acgtcgatgt gagcgatgcc actgcggcga
     1921 tgttcgcgca gcgtgccgtg ccaccggggc gcacgcttgc gcgcttcgtg cactacaaca
     1981 tctggcgtgc gctttccccg ccgccgcagg acgtgccgct ggcagtctgt gatgcgcgca
     2041 gcgtggcggc tgccgatctc attgcagcgg acgcagtgtt cgactcgccg ggaaagccgg
     2101 agtggtcgtt cgaaggcctg ctgctcgccc acgccgccgc gcatcgctgg cattggtttc
     2161 cagacatgca gcgagacgag gcactgatct tcaagaccca tgacagcgac cgcggccgcg
     2221 cgcactgcgt gccgcacgtg gccttcgatc acacggcatg tcccgcgcac gccgctccgc
     2281 gggtgagtat cgagatgcgc gcgctcgcgt tgtggttcgc ctgatcctgt caacctgacg
     2341 gagggtgccg tgcaccgctt tccgaataca ccggagttca ccggcctcaa cacgccaatt
     2401 ggcgccgagt acgagctcgc tgcgctgccg gtcgagggac gcattccaga ggaggtcgcg
     2461 ggcaccttct tccgcgccat tcccgacccg gctttcccgc cgtacatcga agatggcgct
     2521 gccgtactct cgggcgatgg catggtgtcg gcgctgcgct ttgcgccggg caaggtcagc
     2581 ttcgccgtgc gcttcgtcga gaccgagcgg caccgggccg aggtgcgcgc cggccgggcg
     2641 ctgttcggca agtatcgcaa tccgttcacc gaccgcccgg aagccgcggg gctcgaccgc
     2701 acggtcgcca acacgacgcc ggtgtggcac gccgggcgtc tgctcatgac caaggaggat
     2761 ggccgcccgt accgcatcga tccgcacacg ctcgccacgc tcgggcgcta cgacttcggc
     2821 gggcgcctca agagcgcaac ggtgacggca cacgtgcgca tcgatccgca caccggcgag
     2881 ctgtttttct tcgggtatga agccgatggg ctcgcctcga ccacagtctc ttattgtatt
     2941 gcggacgccc gcggcaacct gacacgcgag cagtggttcg atgcgcccta ctgcgcgatg
     3001 atgcatgact tcaccatcac cgagcgctat gcgctcttcc cgatctaccc gacgacctgc
     3061 gatctcgagc gcctgaggtc aggcggcgat cactgggtac acgagttgca gcgcgacagt
     3121 tgggtcggcg tcatgccgcg ctacggatcg gtcggcgagc tgcgctggtt ccggggaccg
     3181 aagggcgtgt cctgctatca catgatgaat gcgttcgagg acgccgacgg ccgcatccat
     3241 ctcgatcaat gtctcgcgaa cgtcaatgcc tttcccttca ttcagcgtgc ctcaggtctc
     3301 gatgtggcgc cgggggacac cggcgcacgc ctcgcgcgct ggaccatcga cctgcgcgat
     3361 ggcaccgagc gcgtgagcga gagcacgatc ggcccgcccg gagatctgcc ggtgattccg
     3421 gcggcgagtc agggccgtcc ctacggccac gggtggctgc tcaccatgaa ccccgacatg
     3481 cagggcccgc cggtggcggg cggcccggtg ggcgtgatgt tcaatacgct gttgcggctc
     3541 gacttcaccg gcgggccgcc gcaggcgctc gcgctgccgc ccggacattg cctgagtgag
     3601 ccggtgcacg tgccgtcttc gcagtccgga cacgaggggt ggctcctcac catcgtcgac
     3661 cggcaggtga gcgagtccga tttcgctcac acgctgtgga tcctcgaggc cggcaacctc
     3721 gctgcgggtc ctatcgcgcg ggtgcccatt gcacaccggc tgcgcccgca ggtgcacggc
     3781 tggtgggtaa gcgcgggcga gctcgccgcc gcgggttgac cgcggcgtca ggtattcgag
     3841 aaatacgcca tcgcgcggaa ctccatcgag cggcgcggcc gggcgtccgg gcgggtcgtg
     3901 tcgcggaagg cggtgtgtgg acagcgccag gccttcgagt gatccgagtc gtagagcttg
     3961 atgaagatca cctcgtccgg ctccatgtcc gggaagtacc accagcgatg cccgggcgag
     4021 tagtgaaaga tggtcgcggc gagcatgtcc tcctcgcccg cgatcggcgc atagagcgcc
     4081 tcgcccgtgg ggatttcggt cacatcgacc ttggtgttgt gggtgccttc ctcatcgcgc
     4141 accgagcggc cctcgcacag cgccagcggc atgtcctgcg gcggcgccga taacgcgcgc
     4201 cacaggctga agccgatgaa gcgccggtag ccggaagcct cggggtatgc ctgacggtag
     4261 agcgccgcag cgcgacgctc cgcgcaacgc tcggtgaaat cgacgtgcgc ctcggcggcg
     4321 ggtggctgca cggtgggact cgtgactgcg gagtcgcgca gcatgccacc gagcggtatc
     4381 acgaggtccg ctccgctcag gctctttgcg acctcggcca cttccgccgc gtaggccgag
     4441 ccgggcgcgt actggttctc ccagtcgccg atggcgctgc gatgccgagc gagacagaag
     4501 ccgtgcgcat cgagcgtaaa gggtccggcg aggcgcgcgt tgcggatggt gacggggtag
     4561 ggcgcataca cacccgtatt cagttcccgc cccggggccc agaagcggcg gttgatgcgc
     4621 gagctcggca ccaggtagtc gatcttcgac tcgacttcag cgcccgcgag ggtcatgtcc
     4681 acctcatccc gctttgaacc cttcgcgcgc gcggctgttg tcgagcacga tcggcttgcc
     4741 cgtcaccgga tgcggatagt ggaaggcgac cgtatcggtc gcgggccaca gccgcggctg
     4801 cgggttcatg agctcgtccg gtccgagagg atcctgcggg tagagcgtct tcgccatagg
     4861 cacaaaatcg atcggggttt tcggccagcc ttcggcgaag ctgccgtgcc agaacgggta
     4921 gtagcgcagc gccttgatct tccacacccc gttctcgcgc aggtacgtgt tttcatagat
     4981 gccaccctcc caccaggcac gttgctgacc ctcggccgtg tgatgtagac cggcctgcat
     5041 catcgagcgc ccgcgcacgt tcgccgtgcg ccggtcgggt gcgacatcga tcaccatctg
     5101 catctgcggg tgatcgagca gccagccgta gcgcgggccg ttgtgtccct gggtgaactg
     5161 cttctgg
//
LOCUS       DATDMP010000631         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1209915, whole genome shotgun sequence.
ACCESSION   DATDMP010000631 DATDMP010000000
VERSION     DATDMP010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209915"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VI195_11105"
     CDS             <1..275
                     /locus_tag="VI195_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824981.1"
                     /translation="TSPLDGVRDPPAALELARALAAGAIRVDPQMYETVAAAYAANGD
                     FKNAAAQQQLAVQTARHLHWDTRTIAERLDEYRRGTVWRGDLLAAR"
     gene            381..689
                     /locus_tag="VI195_11110"
     CDS             381..689
                     /locus_tag="VI195_11110"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY6824982.1"
                     /translation="MPPSQFASFREFYPYYLQQHTNPTSRRLHVAGTLLAVAVAIAAV
                     AAGRWLWLLAAPLAGYLPAWIGHALFEHNTPATLRHPLYSLRGDFTLLLEVLSGRRDW
                     "
     gene            714..>2185
                     /gene="gshA"
                     /locus_tag="VI195_11115"
     CDS             714..>2185
                     /gene="gshA"
                     /locus_tag="VI195_11115"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY6824983.1"
                     /translation="MSRPGIDRVFERRLAALLSGGEPQRLQGGHKGVEKESLRVLPSG
                     GLAHTAHPPALGSALTNEHITTDYSESLIELVTPAFPHSWELLQYLLDLHQFVYRHLG
                     EELLWATSMPCAITSDADIPLAQYGSSHIGRMKTVYRNGLGLRYGRMMQAISGVHFNY
                     SFPQPFWETWAAARGASGHGQPFISACYFDLLRNYRRYGWLVLYLFGVSPVVCKSFLR
                     GRRFGLEDFSPGTAFAPYATSLRMSDVGYRNRNQAGLAVSVNNLEEYVHDLTRAITTP
                     YAPYQALGVEVNGEWRQLNANILQIENEYYSFIRPKRVARSGERPTKALRRAGVEYVE
                     VRALDVSAFDPVGVNQNKLRFLEAFLALCLMKESAPIAPAEQGTLDENHLTVARRGRE
                     PGLTLWREGRSVPMREWARELLDGMSGICEVLDHGDAARPYRQALATQAAKIEDVALT
                     PSARLLAELAAGESFFDLALRMSATHKAYFLDLYPPNDEQL"
BASE COUNT          338 a          752 c          738 g          357 t
ORIGIN      
        1 cgacctcgcc gctcgatggc gtgcgcgacc cgccggcggc gctcgagctc gcccgggccc
       61 tggcggccgg agcgatcagg gtcgacccgc agatgtacga gaccgtggcc gccgcttacg
      121 cggccaacgg cgacttcaag aacgccgctg cacagcagca gctcgccgtg cagacggcgc
      181 gccacctgca ctgggacacg cgcacgattg ccgagcggct ggatgagtac cgccgcggca
      241 cagtgtggcg cggggacctt ctcgccgcgc ggtagctgcc ggggtcggtc gcagtgttgg
      301 cgcggtgtgc ggccgcgggg catcgaggct acccggttga tttgcgcgga cgaggggcga
      361 gacggcaaga tgcccgagca atgccgccgt cacagtttgc gagctttcgc gagttctatc
      421 cttattatct gcagcagcac accaacccaa cctcccgtcg cctgcacgtc gccggcacgc
      481 tgctggctgt cgccgtggcg atcgccgccg ttgccgcggg tcgctggctg tggctgttgg
      541 cggcgccgct cgcgggttac ctgccggcat ggatcgggca cgcgctgttc gagcacaaca
      601 cgccggccac cttgcgccat ccgctctaca gtctgcgcgg cgacttcacg ctgctcctcg
      661 aggtgctcag cggccgccgc gactggtagc gcacccggcg gcgttgccca ctcatgagcc
      721 ggcccggtat cgaccgcgtg ttcgaacgcc ggctggcggc gctgctgagc ggtggcgagc
      781 cgcagcggct gcagggcggc cacaagggcg tcgagaagga gtctttgcgc gtgctgccct
      841 cgggcggact ggcgcacact gcgcatccgc ccgcgctcgg ctccgcgctc accaacgagc
      901 acatcaccac cgattactcg gagtcactga tcgagctggt cacgcccgcg tttccgcaca
      961 gctgggagct gctgcagtac ctgctcgacc tgcaccagtt cgtctaccgg cacctcggcg
     1021 aggagctgct gtgggcgacc agcatgccct gtgcgatcac gagcgatgca gacatcccgc
     1081 tcgcgcagta cggcagctcg cacatcggcc gcatgaagac ggtgtaccgc aacggcctcg
     1141 gcctgcgtta cggacgcatg atgcaggcga tctcgggcgt gcacttcaac tattcctttc
     1201 cgcagccgtt ctgggaaacc tgggcggcgg cgcgcggcgc ctccggacac ggccagccgt
     1261 tcatatccgc gtgctatttc gatctgctgc gcaactaccg ccgctacggc tggctggtgc
     1321 tgtacctgtt cggggtttcg ccggtggtgt gcaagtcgtt tctgcgcggg cgccgcttcg
     1381 ggctcgagga tttcagcccg ggcaccgcct tcgcgccgta cgccaccagc ctgcgcatga
     1441 gcgatgtcgg ctaccgcaat cgcaaccagg cggggttggc ggtgtcggtg aacaatctcg
     1501 aggagtatgt gcacgacctg acgcgcgcga tcaccacgcc ttatgcgcca taccaggcgc
     1561 tcggggtcga ggtgaacgga gagtggcgcc agctcaacgc caacatcctg cagatcgaga
     1621 acgagtacta cagcttcatc cgccccaagc gcgtggcgcg ctcgggcgag cgccccacca
     1681 aggcgctgcg ccgggcgggc gtcgagtacg tggaggtgcg cgcgctcgat gtgagcgcat
     1741 tcgacccggt cggcgtcaac cagaacaagc tgcgcttcct cgaagctttc ctcgccctgt
     1801 gcctgatgaa ggagagtgcg cccatcgcgc ccgccgaaca gggaacgctg gacgagaacc
     1861 acctcaccgt ggcgcgacgc ggccgcgaac cgggcctgac gctgtggcgc gaaggacgca
     1921 gcgtgccgat gcgcgaatgg gcacgggagt tgctcgatgg catgagcgga atctgcgaag
     1981 ttctcgacca cggtgatgcg gcgcggcctt accggcaggc gctcgccact caggcggcca
     2041 agatcgagga cgtggcgctc accccgtcgg cacggctgct ggccgagctg gccgcgggcg
     2101 aatcgttctt cgatctggcg ctgcgcatgt ccgcgaccca caaggcttat tttctcgacc
     2161 tgtacccacc caacgacgag cagct
//
LOCUS       DATDMP010000632         4106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_987976, whole genome shotgun sequence.
ACCESSION   DATDMP010000632 DATDMP010000000
VERSION     DATDMP010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987976"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VI195_11120"
     CDS             <1..1427
                     /locus_tag="VI195_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HEY6824984.1"
                     /translation="PGLQIVDPELFNSLTSLHGLVMIFGAIMPAWVGLANWMIPIQIG
                     APDMALPRLNNLSFWILPFAFTLLIGSFFVPGGAPAGGWTMYPPLILQTGAAFPMLIF
                     AVHLMGASSILGAINVIVTIMNMRAPGISLLRMPLFVWTWLITAYLLIAVMPVLAGAV
                     TMLLTDHFFGTTFFTAAGGGDPVMFQHIFWFFGHPEVYILILPAFGIVSEVIPTFSRK
                     PLFGYETMVYATASIAFLSFIVWAHHMFTVGMPLAAQLFFMLSTMLIAVPTGVKVFNW
                     TATMWKGSLSFEPPMLFALAFLFLFTIGGFSGLMLAIVPADYQYQDTYFVVAHFHYVL
                     VPGTIFGIMAGVYYWLPKWTGRMYDLTLATWHFWLSTIFVNVLFFPQHFLGLAGMPRR
                     IPDYAVQFTDWNMVSSLGGFGFGLSQLLFPYLIWKCVRSGVPIGNRAWEGAHGLEWEL
                     PSPPPHHSWTEPPSDAVIARGCAH"
     gene            1427..1561
                     /locus_tag="VI195_11125"
     CDS             1427..1561
                     /locus_tag="VI195_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824985.1"
                     /translation="MAAAGLEASAQQRRRVRRSALLWALIAAAFYLGFIVMALMRGAK
                     "
     gene            1558..2103
                     /locus_tag="VI195_11130"
     CDS             1558..2103
                     /locus_tag="VI195_11130"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY6824986.1"
                     /translation="MSDRRRANLVMTWKLLAIAAGSFAFGFALVPLYNVLCSVTGYGD
                     QAKLRERVSAVERPQLDRTVTVQFLTDVASAGTFEFRPVVRTVEVHPGQLFTAQFYAH
                     NLSGRATTAQAVPNIAPSEVAAYFHKTECFCFSPQHFALDEGRDMPVRFIIDPSLPRH
                     IDLITLAYTFYDESARVTARR"
     gene            2117..3004
                     /locus_tag="VI195_11135"
     CDS             2117..3004
                     /locus_tag="VI195_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY6824987.1"
                     /translation="MANAHASDSNAYYIPHGSPWPIRASLALFAMMLGAVGYLNDWAE
                     GWIFLPGIVLLAYMFFGWFSTVIGENQHGIYNLQVDRSFRMGMMWFIFSEVMFFGAFF
                     GALFYARVLAVPWLGGEGVKVFTNFVLWPQFEAVWPTNGPAHVGGLANGVFTPMAPTP
                     IPAINTAILLTSGLTVTIAHHALRSGHRGRLALFLGLTFLLGFTFVGLQAHEYGEAYR
                     DLGLRLTTGIYGSTFFMLTGFHGLHVTIGAIMLTVIWLRVLRGHFTPNRHFAFEAVAW
                     YWHFVDVVWLGLFIFVYWL"
     gene            complement(3023..3256)
                     /locus_tag="VI195_11140"
     CDS             complement(3023..3256)
                     /locus_tag="VI195_11140"
                     /inference="COORDINATES: protein motif:HMM:NF022587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY6824988.1"
                     /translation="MEHLVWLLVLACLLGILVSLGLGLYHLSRGTPQDSARLARALTV
                     RIGVSLLLFVLLMLAWYFGLISPHPLQGAAAPN"
     gene            3302..4045
                     /locus_tag="VI195_11145"
     CDS             3302..4045
                     /locus_tag="VI195_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY6824989.1"
                     /translation="MTAPPGPGRRVFAPTLRLTLLAVLLGALFVRLGLWQWQRGEQRQ
                     AAATHFARGADQVLELGTGTGSELPLYQRVRVEGELDAAHQFLLDNRSFEGHPGYEVL
                     TPLTRAGTPALLVDRGWVPFTGSRARLPDVSLRVSHRVDLTGRIAQLPTAGLALGRAA
                     PSARDPWPKVTSYPDMAQLAAALGAPLAPRILLLDPAAPLGYVRAWQPPGLPPLRHFS
                     YAIQWWTFALLTCALWVFLSRRPRQAAAS"
BASE COUNT          571 a         1459 c         1291 g          785 t
ORIGIN      
        1 agccgggact gcagatcgtc gaccccgagc tgttcaattc cctcacctcg ctgcacggcc
       61 tggtgatgat cttcggcgcc atcatgccgg cgtgggtggg gctcgccaac tggatgatcc
      121 cgattcagat cggggcgccc gacatggcgc tgccgcgcct caacaatctc agcttctgga
      181 tcctgccgtt cgcattcacg ctgctgatcg gctcgttctt cgtgcccggc ggcgcgccgg
      241 cgggcggctg gaccatgtat ccgccgctca tcctgcagac cggcgccgcc ttccccatgc
      301 tcatcttcgc ggtgcatctg atgggcgcct cctcgatcct cggtgccatc aacgtcatcg
      361 tcaccatcat gaacatgcgt gcgcccggca tatcgctgct gcgcatgccg ctgttcgtgt
      421 ggacctggct gatcaccgcg tacctgctga tcgcggtgat gccggtgctc gccggcgcgg
      481 tcaccatgct gctcaccgat catttcttcg gcaccacctt cttcaccgcc gcgggcggcg
      541 gcgatccggt gatgttccag cacatcttct ggttcttcgg tcaccccgag gtgtacatcc
      601 tcatcctgcc ggccttcggc atcgtctccg aggtgatccc gaccttctcg cgcaagccgc
      661 tgttcggcta cgagaccatg gtgtacgcga ccgcctcgat cgccttcctg tcgttcatcg
      721 tctgggcgca tcacatgttc acggtgggca tgccgctcgc cgcgcagctg ttcttcatgc
      781 tctcgaccat gctgatcgcg gtgccgaccg gcgtgaaggt gttcaattgg accgcgacca
      841 tgtggaaagg ctcgctgtcc ttcgagccgc cgatgctgtt cgcgctcgcc ttcctgttcc
      901 tgttcaccat cggcggcttc tccgggttga tgctcgccat cgtgccggcg gactaccagt
      961 accaggacac ctacttcgtg gtggcgcact tccactacgt gctggtgccg ggaaccatct
     1021 tcggcatcat ggccggggtg tattactggc tgccgaaatg gaccggccgg atgtatgacc
     1081 tgacgctggc gacatggcac ttctggctgt cgaccatctt cgtgaacgtg ctgttcttcc
     1141 cgcagcactt cctcgggctc gccggcatgc cgcgccgcat tcccgactac gccgtgcagt
     1201 tcaccgactg gaacatggtg tcctcgctcg gcggcttcgg cttcgggctg tcgcagctgc
     1261 tgttcccgta cctcatctgg aagtgcgtgc gctccggcgt gccgatcggc aaccgcgcgt
     1321 gggagggcgc acacggactc gagtgggaac tgccctcgcc gccaccgcac cactcgtgga
     1381 cggagccgcc ctcggacgcg gtgatcgcgc gcgggtgcgc acactgatgg ctgccgccgg
     1441 cctggaagcg agcgcacagc agcgccggcg cgtgcggcgc agcgcgctgc tgtgggcgct
     1501 gatcgcggcg gcgttctacc tcggcttcat cgtcatggcg ctgatgcgcg gcgcgaaatg
     1561 agcgaccggc ggcgcgccaa cctcgtcatg acctggaagc tgctcgccat cgctgccggc
     1621 agcttcgcgt tcggcttcgc gctcgtgccg ctctacaacg tgttgtgctc ggtcaccggc
     1681 tacggcgacc aggccaagct gcgcgagcgc gtgagcgcgg tcgagcgccc gcagctcgac
     1741 cgcaccgtga ccgtgcaatt cctgacggac gtcgccagcg ccggcacttt cgagttccgc
     1801 ccggtggtgc gcacggtcga ggtgcacccg gggcagttgt tcaccgcgca gttctacgcg
     1861 cacaacctga gcggccgcgc taccaccgcg caggcggtgc ccaacatcgc cccctccgag
     1921 gtcgccgcct acttccacaa gaccgagtgc ttctgcttct cgccgcagca cttcgcactg
     1981 gatgaggggc gcgacatgcc ggttcgcttc atcatcgatc cgtcgctccc gcgacacatc
     2041 gacctgatca ctctcgctta caccttctac gacgaatccg cgcgcgtcac ggcgcgccgc
     2101 tagaaagcac atcgacatgg ccaacgcaca cgcatccgac agcaacgcct actacatccc
     2161 gcacggcagt ccctggccga tccgcgcatc gctggcgctg ttcgccatga tgctcggcgc
     2221 ggtcgggtat ctgaacgact gggcggaggg ctggatcttc ctgccgggca tcgtgctgct
     2281 cgcctacatg ttcttcggct ggttctcgac cgtcatcggc gagaaccaac acggcatcta
     2341 caacctgcag gtcgaccgca gcttccgcat ggggatgatg tggttcatct tctccgaggt
     2401 catgttcttc ggcgcctttt tcggcgcctt gttctacgcg cgggtgctcg ccgtgccgtg
     2461 gctcggcggg gagggcgtga aggtgttcac caacttcgtc ctctggccgc agttcgaggc
     2521 ggtgtggccc accaacggac cggcgcacgt cggcggtctc gccaacgggg tcttcacccc
     2581 catggcgccg acgcccatcc ctgcaatcaa caccgccatc ctgctcacca gcggtctcac
     2641 cgtgaccatc gcgcatcacg ccctgcgctc cggccaccgc ggtcgcctgg cgctgttcct
     2701 cgggctcacc ttcctgctcg gtttcacctt cgtgggacta caggcccacg agtacggcga
     2761 ggcctaccgc gacctgggcc tgcggctcac gaccggcatc tacggctcga ccttcttcat
     2821 gctcaccggg ttccacggcc tgcacgtcac catcggcgcg atcatgctga cggtcatctg
     2881 gctgcgcgtg ctgcgcgggc atttcacgcc caaccgccac ttcgctttcg aggcggtcgc
     2941 ctggtactgg cacttcgtcg atgtggtgtg gttagggctg ttcatcttcg tctactggct
     3001 gtagagcgcc ccgttcacgg cgtcagttcg gtgcagccgc cccctgcagc gggtggggcg
     3061 agatgagacc gaagtaccag gcgagcatca acaacacgaa cagcagcagc gatacgccga
     3121 tgcgcacggt gagcgcgcgc gcgagccgcg cggaatcctg cggagtgccg cgcgagaggt
     3181 ggtacaggcc gagccccagg ctgacgagaa tgccgagaag acacgccagc accagcagcc
     3241 agacgagatg ttccatgcgt ggcagtcagt gtagcgtgcg cagagcgcgg agcaatttgc
     3301 cgtgaccgcg ccgccaggcc cggggcggcg ggtgttcgcg cccacgctgc gcctgacgct
     3361 gctggcggtg ctgctcgggg cgctgttcgt gcgcctgggt ttgtggcagt ggcagcgggg
     3421 cgagcagcgc caggcggcgg cgacgcactt tgcgcgcggc gccgatcagg tgctcgagct
     3481 cggcaccggt accggaagcg agctgcctct ctaccagcgc gtccgcgtcg agggcgagct
     3541 cgatgccgcg caccagttcc tgctcgataa ccgcagcttc gaggggcacc cgggctacga
     3601 ggtactcact ccgctcaccc gtgcgggcac accggcgctg ctcgtcgacc ggggttgggt
     3661 acccttcacc ggcagccgcg cccggcttcc ggatgtgagt ctgagagtga gtcaccgcgt
     3721 ggacctgacc ggccgcatcg cacagctgcc cacggcgggg ctggcgctcg gacgtgccgc
     3781 cccctcggct cgagatccat ggcccaaggt gacgagttac cccgacatgg cgcagctcgc
     3841 cgccgcgctc ggcgcgccgc tggcgccgcg cattctgctg ctcgatcccg ccgccccact
     3901 gggttatgtg cgcgcctggc aacctcccgg gcttcctccg ctgcggcatt tctcgtacgc
     3961 catccagtgg tggaccttcg cgctgctcac ctgcgcgctg tgggtgttcc tgagccgccg
     4021 cccgcggcag gcggcggcct cgtgagcgtc atcagtccga gcgagctgcg cgcgcgcact
     4081 ctgcgcaccc tcatgctgct cgcgac
//
LOCUS       DATDMP010000633         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_544078, whole genome shotgun sequence.
ACCESSION   DATDMP010000633 DATDMP010000000
VERSION     DATDMP010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544078"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2140)
                     /gene="acnA"
                     /locus_tag="VI195_11150"
     CDS             complement(<1..>2140)
                     /gene="acnA"
                     /locus_tag="VI195_11150"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY6824990.1"
                     /translation="NPQAFANNTLLEYERNQERYQFLRWGQNAFRNFKVVPPETGIVH
                     QVNLEYLARVVFDAERSGVRRAYPDTLLGTDSHTTMVNGLGVLGWGVGGIEAEAAMLG
                     QPVTMLIPQVIGFRLEGRLQPGATATDLVLTVTEMLRKKGVVDKFVEFCGDGLANLPL
                     ADRATIANMAPEYGSTCGIFPIDAETVRYLELSGRARERIELVSNYARAQGLWRNQGA
                     GTARYSDELELDLAKVEPSLAGPRRPQDRVPLRTARQVYRSGARKAADERTARNPAAA
                     GVATAELDGQTVELRDGAVLIAAITSCTNTSNPSVMVGAGLLARKARERGLTSRPWVK
                     TSLAPGSRVVTDYLTKAGLLDDLAALGFNLVGYGCTTCIGNSGPLKPEISAAVKAGDL
                     TACAVLSGNRNFEGRVHPEVRMNFLASPPLVVAYALAGSLDVDLTSEPLGTGSDGKPV
                     QLADIWPSDAEVQEAIKRSVDSQMFRSSYASVFKGDENWAGIEVPAGQRYRWDPQSTY
                     VKHPPYFAGMTATPPPLADVRGARVLALLGDSVTTDHISPAGNIARSSPAGRYLIEQG
                     VQPQDFNSYGARRGNHEVMVRGTFANIRLRNLLVPGVEGGVTVHLPDGGQAAIYDAAM
                     RYREEGVPLVVLAGREYGTGSSRDWAAKGTMLLGVKAVIAESFERIHRSNLVNMGVLP
                     LQFPNGVTAASLGLIGRETYDLVGIAQGLKP"
BASE COUNT          292 a          727 c          787 g          334 t
ORIGIN      
        1 cgggcttcag cccctgcgcg atgccaacca ggtcgtacgt ctctcggccg atcagtccga
       61 gcgacgccgc ggtcacgccg ttcgggaact gcagcggcag cacgcccatg ttcacgagat
      121 tgctgcggtg gatgcgctcg aagctctcgg cgatgacggc tttcacgccg agcagcatcg
      181 tgcccttcgc ggcccagtcc cgcgaggagc cggtgccgta ctcgcgcccg gcgagcacca
      241 cgagcggcac gccttcctcg cggtagcgca tcgcggcgtc gtagatcgcg gcctgtccgc
      301 cgtcgggcag gtgcaccgtg accccgccct cgacgccggg caccagcagg ttgcgcagcc
      361 ggatgttggc gaaggtgccg cgcaccatca cttcgtgatt gccgcggcgc gcgccgtagg
      421 agttgaagtc ctggggctgc acgccctgct cgatcaggta gcgcccggcg ggactgctgc
      481 gcgcgatgtt gccggccggc gagatgtggt cggtggtgac cgagtcaccg aggagcgcga
      541 gcacccgcgc cccgcgcacg tcggcgagcg gcgggggtgt ggcggtcatg cccgcgaagt
      601 acggcgggtg cttcacgtag gtcgactgcg ggtcccagcg gtagcgctgg ccggcgggca
      661 cctcgatgcc cgcccagttc tcgtcgccct tgaaaacgct cgcgtagctc gagcggaaca
      721 tctgcgaatc gaccgagcgc ttgatcgcct cctgcacttc ggcatcgctc ggccagatgt
      781 cggcgagctg caccggcttg ccgtcactgc cggtgccgag cggctcgctg gtgagatcga
      841 cgtcgagcga gccggcgagc gcgtaggcga ccacgagcgg cggggaggcg aggaagttca
      901 tgcgcacctc ggggtgcacc cgtccctcga agttgcggtt gccggacagc accgcgcagg
      961 cggtgaggtc gccggccttg accgccgcgg agatctcggg cttgaggggg ccggagttcc
     1021 caatgcaggt agtgcaaccg tagcccacga ggttgaagcc cagggcggcg agatcatcga
     1081 gcaggccggc cttggtgagg taatcggtca ccacgcgtga cccgggcgcg aggctcgtct
     1141 tcacccacgg cctggaggtg agcccgcgct ctcgcgcctt gcgcgccagc agtcccgcgc
     1201 cgaccatgac cgagggattc gaggtgttgg tgcagctggt gatcgccgca atcagcaccg
     1261 cgccgtcgcg cagctcgacc gtctggccgt cgagctcggc ggtcgcgacg ccggcggcgg
     1321 cgggattgcg cgcggtgcgc tcgtcggccg ccttgcgggc gccgctgcgg tacacctggc
     1381 gcgcggtccg gagcgggacg cggtcctgcg gacggcgcgg gccggcgaga ctcggctcca
     1441 ccttcgcgag gtcgagctcg agctcgtcgc tgtagcgggc ggtgcccgcg ccctggttgc
     1501 gccacaggcc ctgggcccgg gcgtagttgc tcaccagctc gatgcgctcg cgcgcgcggc
     1561 cggagagctc gaggtagcgg accgtctccg cgtcgatggg gaagatgccg caggtgctgc
     1621 cgtactcggg agccatgttg gcgatggtgg cgcggtcggc gagcggcagg ttggcgaggc
     1681 cgtcgccgca gaactccacg aacttgtcga ccacgccctt cttgcgcagc atctcggtga
     1741 ccgtgagcac caggtcggtg gcggtcgccc ccggctgcag ccgtccctcg aggcgaaagc
     1801 cgatcacctg cgggatcagc atggtcaccg gctggccgag catggcggct tcggcctcga
     1861 tgccgcccac cccccagccg aggacgccga ggccgttgac catggtggtg tgcgagtcgg
     1921 ttccgagcag cgtgtcggga taggcgcggc gcacgccgga gcgctcggcg tcgaacacca
     1981 cccgcgcgag gtactcgaga ttcacctggt ggacgatgcc cgtctcgggc ggcaccacct
     2041 tgaagttccg gaaggcgttc tgcccccagc ggaggaactg gtaccgctcc tggttccgct
     2101 cgtattccag cagcgtgttg ttggcgaacg cctgcgggtt
//
LOCUS       DATDMP010000634         2749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_211035, whole genome shotgun sequence.
ACCESSION   DATDMP010000634 DATDMP010000000
VERSION     DATDMP010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211035"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /gene="mobQ"
                     /locus_tag="VI195_11155"
     CDS             complement(<1..938)
                     /gene="mobQ"
                     /locus_tag="VI195_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HEY6824991.1"
                     /translation="MAHFYMQISSITRAAGRRATAAAAYRSGERIRDERTGEVINHSR
                     RRDVLHTEIFVPTQFEGMGVAWARNRERLWNAAEHAEKRHNARVAREYQVTLPVELDA
                     TRRVALARAFAQEIAERYKVAVDLAVHEPRVDGDVRNFHAHLLTTTREVTPAGLGAKV
                     GLDMHPQERRRHGLPDHRQEYINVRERWATLTNEALREANIDARIDHRSLAAQGVDRE
                     PGPAIPLMHLKMERRGLQSELAERLRAEYRERVARRLENTAARDARVSAPGEKSAAER
                     AAATSGPTADAAEIRRRAREAWLQLRAGQPTHPDES"
     gene            complement(942..2324)
                     /locus_tag="VI195_11160"
     CDS             complement(942..2324)
                     /locus_tag="VI195_11160"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEY6824992.1"
                     /translation="MIDWSEGFTQLREWLLWPLCAVIVLIGPLALRMRRHDIHRRGAR
                     VLERRQLGRVLQGRRRGASLAIAGVPVLQGEETRHFKLIGTTGTGKSTAISGLLKAAL
                     ERGDRAVIADPDGGYRAQFYDRRRGDVVLNPFDPLSVKWDPFTEIRAPWDVEQLTSGL
                     IPSSEDPSGREWRGYARTFLTAVTRRCCETGCHDSSELWRLLTVATSSELRALVAGSP
                     AQPFLEPENARMFGSIRSVAGSAAVALQYVQAQRARSFSVRDWVRCGRGVLFIPYGAA
                     QIAALRSIIATWVRLAIFEVMGRTGEGPPLWFVVDELDSLGAIDGLKDALARLRKFGG
                     RCVLGFQSIAQVSATYGFGEAQTLVENCGNTLILRCSGSEHGGTSQFASRLIGEREVV
                     RRQTSRAHDRAGWLAAGSARRSTTVSRQQVLETAVLAAEIEQLPDLAGFLKTAASPVW
                     LKVTVPRGGG"
     gene            complement(2337..2714)
                     /locus_tag="VI195_11165"
     CDS             complement(2337..2714)
                     /locus_tag="VI195_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824993.1"
                     /translation="MREEIRATLVEEMRALGEDSQRAAAALRRLQRSANLRLIVWGAA
                     VLSMAATLPCALAWWLLPTRAEIAALGARRAELSANIARLSSQGGNVELRHCGTEQRL
                     CVRIERSAPAYGASGDFRVIKGY"
BASE COUNT          497 a          946 c          820 g          486 t
ORIGIN      
        1 aacgattcgt cagggtgcgt tggctgcccc gcgcgcagct gcagccaggc ttcccgagcc
       61 cgccgtctga tctccgccgc gtccgcagtc ggaccggaag ttgcagcagc tcgctcggca
      121 gctgattttt caccgggggc actgacccgg gcgtctcgcg ccgccgtgtt ttccagccgc
      181 cgcgcaaccc gttcgcgata ttcggcgcgc agccgctcgg caagctcact ttgcaggccg
      241 cggcgttcca ttttcagatg catcaacgga atcgccggcc ctggctcgcg atctacaccc
      301 tgcgccgcca ggctgcgatg atcgatgcgc gcatcaatat tggcttcacg cagtgcctca
      361 ttggtgagcg tcgcccaccg ctcacggaca ttgatgtact cttgccgatg atccggcaaa
      421 ccatggcgtc gccgctcctg cggatgcatg tcgagcccca ccttggcgcc gagtcctgcc
      481 ggggtgactt cacgagtcgt cgtcaacaaa tgcgcatgga agttccgcac atcgccatcc
      541 acacgcggct catgaaccgc aagatccacc gctaccttgt agcgttccgc gatctcctgt
      601 gcgaaggctc gcgccaaggc gacgcgccgc gtggcatcga gctccaccgg cagggtgacc
      661 tggtactcgc gtgccacgcg cgcgttgtgg cgtttttcag catgctcggc cgcattccac
      721 aggcgttccc gattccttgc ccacgctacc cccataccct cgaattgcgt tggcacgaaa
      781 atttcagtgt gcaacacgtc ccggcgcctg gagtgattga tcacctcccc ggtgcgctcg
      841 tcgcggatgc gctccccgga gcgatacgcg gcagccgcag tggcgcgccg gccagcggcg
      901 cgggtaatgg agctgatctg catgtaaaag tgagccatgc ttcagcctcc accacgcggc
      961 acggtcacct tcaaccacac cggcgacgca gcggtcttga gaaatccggc tagatccggc
     1021 agctgttcga tctcggcagc aagcacggcc gtctcgagaa cctgctgtcg acttacggtc
     1081 gttgagcggc gggcgctgcc ggccgcgagc cagccggcgc gatcgtgtgc gcgggaggtt
     1141 tgcctgcgca cgacctcgcg ctcgccgatt aggcgcgagg cgaactgcga tgtgccgccg
     1201 tgctcgcttc ccgagcagcg taaaatcagg gtattgccgc agttttctac aagcgtctgt
     1261 gcttctccaa atccatatgt ggcggagacc tgagcaatcg actgaaaccc gagcacgcac
     1321 cgtccgccaa atttgcgtag gcgcgccagc gcatctttga ggccgtcgat cgcgccaagc
     1381 gagtccagct cgtcgacgac gaaccacaat ggcgggcctt cacccgtccg gcccatcacc
     1441 tcgaaaatgg ccaggcgcac ccaggtggca atgatggagc gcagcgctgc gatctgagcc
     1501 gctccgtacg gaatgaacag cacaccgcgc ccgcagcgca cccagtcgcg tacggaaaag
     1561 gaccgcgcgc gctgcgcctg cacgtactgg agtgccacgg cggctgaccc ggctactgag
     1621 cggatcgagc cgaacatgcg ggcattctcc ggctcgagaa atggctgcgc tggggagcct
     1681 gccacgagcg ctcgaagttc cgagctcgtc gcaacggtca gcaatcgcca gagctcactg
     1741 gaatcatggc atccagtctc gcagcagcgc cgcgttacgg cggtgaggaa cgtgcgcgcg
     1801 tacccgcgcc actcgcgacc ggacggatct tcgctcgagg gtatcaatcc actcgtcagc
     1861 tgctcgacat cccagggagc gcgaatttcc gtgaacggat cccatttcac ggacaacgga
     1921 tcgaagggat tgagaacgac atcgcctcgt cgtcgatcgt agaactgcgc tcgatagcca
     1981 ccatccggat cggcgatcac ggcacggtcc ccgcgctcga gcgctgcttt cagaagaccg
     2041 gagatggcgg ttgacttgcc agtgcccgtg gtgccgatga gcttgaaatg ccgcgtttcc
     2101 tcgccttgca ggacaggcac cccggcaatc gccaggctcg cgcctcgccg cctaccttgc
     2161 aaaacgcggc cgagctggcg tctctcgagc acgcgtgcgc cacgcctatg tatatcgtgc
     2221 ctccgcatgc gcagcgccag gggaccaatc aggacgatga ccgcgcacaa tggccaaagc
     2281 agccattcgc gcagctgtgt gaacccctcg ctccaatcga tcatgccgct gcagcctcaa
     2341 taccccttga tcacccgaaa gtccccgctc gcgccataag cgggtgcgct acgctcgatg
     2401 cgcacacaca gtcgttgctc agttccgcaa tggcgtaatt ccacattgcc gccttggctc
     2461 gaaaggcgtg cgatgttcgc ggaaagttcc gcgcgtcggg caccaagtgc tgcaatctcc
     2521 gctcgtgttg gcagcagcca ccaggccagc gcgcaaggaa gcgttgcggc catgctgagg
     2581 acagccgcgc cccacacgat caggcgcagg ttggccgacc tctgcagccg gcgcagagcc
     2641 gcggcagcac gctggctgtc ttcacccaat gcccgcattt cttcgacgag cgtggcgcga
     2701 atctcctcgc gcacgatcgc atcgagtcct gcggtatgct cgcgcaaac
//
LOCUS       DATDMP010000635         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1101169, whole genome shotgun sequence.
ACCESSION   DATDMP010000635 DATDMP010000000
VERSION     DATDMP010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101169"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1847
                     /locus_tag="VI195_11170"
     CDS             <1..1847
                     /locus_tag="VI195_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY6824994.1"
                     /translation="TIEELTHTVGGLDKDLNLLLTHDNAGLPSAEAGGSDLQQLLQQT
                     IEHLRASAGALLVPEKSVMLVRSADGRPPDTQFWMRAHRRLMPLAQAQREPLVFNETQ
                     SMETPEAFPFRVLCCSLRSRTGRTIGLLALLREPLAEPFGPRDAHVGEILSRKAIAAI
                     ESNYDPLSGLYTRPAFERRLKTVVADAKSTSWSALYIDVDQLHAINENSGMHVGDALL
                     GQLGELIRQRLPPGAFGARISGDRFALLLPARVPDAEAFAESLREGTERLALVSTEAR
                     LPVSISAGVASVDAQASDLAQVLAPAETACKAAKDRGRNRVEVYQPSDASIVRRHADI
                     GVAGQVREAIDSGRLHLDAQLILPFAAAESARPHYELLLRMTDESGRTRGPDNFLSAA
                     TRYQLMPVIDRWVISHVIAALQPRAAILEGKALGFAINVSGQSMNDDAFADYLVKSIG
                     ASGLDPELFCFELTENATVANLARTETLMRRLRQLGCGVALDDFGTGLSSLSCLRQLP
                     VSMLKIDGSFVREVLKDARAESMVRAIAQLARSMSITTVAEYIETEAISARIAELGVD
                     YGQGFAIGKPIPLADLLTELPLPSPSEGDDPDATGKRDQVAWFAGAAR"
     gene            1922..>2235
                     /locus_tag="VI195_11175"
     CDS             1922..>2235
                     /locus_tag="VI195_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6824995.1"
                     /translation="MSSRRTLIALTAGAFLGFCGALTGGVLADRPSAVANAETPARSA
                     LPWQDASLFAEVYERIKRDYVDEVDDHALMAKAIRGMVAALDPHSAYLDSEEFDEIRL
                     ST"
BASE COUNT          358 a          800 c          734 g          343 t
ORIGIN      
        1 ccaccatcga ggagctcacc cacaccgtgg gcggactcga caaggacctg aacctgctcc
       61 tcacccatga caacgccggc ttgccgagcg cggaagccgg cggcagcgac ctgcagcagc
      121 tgctgcagca gaccatcgag cacctgcgtg ccagtgccgg cgcgctgctg gtgcccgaga
      181 agagcgtcat gctggtgcgc tccgcggatg gccggccgcc ggacacgcag ttctggatgc
      241 gcgcccatcg ccggctgatg ccgctcgcgc aggcgcagcg cgagccgctg gttttcaatg
      301 agacccagtc gatggaaacg cccgaggcgt ttcccttccg cgtactgtgc tgctcgttgc
      361 gctcgcgcac cggccgcacc atcggcctgc tggcgctgct gcgcgagccg ctcgccgagc
      421 ccttcggccc acgcgatgcc cacgtgggag agatcctctc gcgcaaggcc attgccgcca
      481 tcgagagcaa ctatgacccc ctgagcgggc tctatacgcg tccggcgttc gagcgacgcc
      541 tgaagacggt ggtcgcggac gccaagtcca cctcctggag cgcgctctac atcgatgtcg
      601 atcagctgca cgccatcaac gagaactccg gcatgcacgt cggcgacgca ctcctcgggc
      661 agctcggcga gctcatccgc cagcggctgc cacccggggc attcggggca cgcatttcgg
      721 gggatcgctt tgcgctgctg ctgcccgcgc gggtaccgga tgcggaagcc ttcgccgaat
      781 ccctgcgcga gggcaccgag cggctcgcgc tggtgagcac cgaggcgcgc ctgcccgtgt
      841 cgatcagcgc cggggtggca tcggtcgatg cgcaggcgag cgatctcgca caggtgctgg
      901 cgccggccga gaccgcctgc aaggcggcca aggaccgcgg ccgcaaccgc gtcgaggtct
      961 accagccgag cgatgccagc atcgtacgcc gccacgctga catcggggtg gcaggccagg
     1021 tgcgcgaggc catcgactcg ggccgcctgc acctcgatgc gcagctcatt ctccccttcg
     1081 ccgccgccga gagcgcgcgc ccgcactacg agctgctgct gcgcatgacc gatgagagcg
     1141 gccgcacgag gggcccggac aacttccttt ccgccgcgac ccgctaccag ctgatgccgg
     1201 tcatcgaccg ctgggtgatc tcgcacgtga tcgcggcgct ccagccgcgc gcggcaattc
     1261 tcgagggcaa agcgctcggc ttcgccatca atgtctcggg ccagtcgatg aatgacgacg
     1321 ccttcgccga ttacctggtg aagagcattg gcgcaagcgg cctggatccg gagctcttct
     1381 gcttcgagct caccgagaac gccacggtcg cgaacctcgc ccgcaccgag accctcatgc
     1441 ggcggctgcg ccagctcggg tgcggcgtgg cgctcgatga tttcgggacc gggctttcct
     1501 cgctctcctg cctgcggcag ctgcccgtca gcatgctcaa gatcgacggc agcttcgtgc
     1561 gggaggtgct caaggacgca cgcgccgagt ccatggtgcg cgcgatcgcg cagctcgcgc
     1621 gcagcatgtc gatcaccacg gtggccgagt acatcgagac cgaggcgatc agcgcgcgca
     1681 tcgcggagct gggggtggac tacgggcagg ggttcgcgat cggtaaaccc attccgctcg
     1741 cggatctgct caccgagctg ccactgccga gtccctctga aggggacgac ccggacgcga
     1801 ccgggaaacg tgaccaggtc gcctggttcg ccggtgcggc gcgttgaagc cgcccgggga
     1861 acccccgccg ccagcgccga tcccaacaac ccttgacgtt gcgaatcgag gtctccacgt
     1921 catgtcatcc cgacgcacgc tcatcgccct gaccgcaggg gctttcctcg gcttctgcgg
     1981 ggctctcacc ggtggcgtcc tcgcggaccg gcccagcgcc gtggcaaacg cggagacgcc
     2041 cgcgcgcagc gcccttccct ggcaggatgc gagcctgttc gccgaggtat acgagcgcat
     2101 caagcgggac tacgtggacg aggtcgatga ccatgccctg atggcgaaag ccattcgcgg
     2161 catggtggca gcgctcgatc cccactccgc ttacctcgac agcgaggagt tcgacgagat
     2221 acgcctctcg accat
//
LOCUS       DATDMP010000636         3752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1989062, whole genome shotgun sequence.
ACCESSION   DATDMP010000636 DATDMP010000000
VERSION     DATDMP010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989062"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="VI195_11180"
     CDS             <1..1815
                     /locus_tag="VI195_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6824996.1"
                     /translation="YRRDGGWVDDINPVTLAPVESDANYVDNYLARLAFLWTPSDKWS
                     LTPAIYYQDSKRHDINNFWPLYSDASNGKFVNADPTQRRVPDKFYLASLKIQGDLGAA
                     QFISNTSYYDRTNQDGYEGTIYNLGFYQIYAFLPPYVSGSYPLIDGNGIHLPPGATNY
                     TSPSTVDNGQINISQEFRLQSSDPNARLLWTTGLFFSENRQSYLEQIHDPYLDQLSLA
                     LTGLHYTDIFVDANGNPVPYVPGFPNDSYFLQTHAKDEQYAIFGEATFAATEKLKLTA
                     GARFSKNKYSFDTYTGGPQLFAAPTTGSGNNSENSFTPKLSIQYQADPNNMFYATYAK
                     GFRPGGANNPLPYAACSQDFQAFGISNAPTTYNSDTVDSFEIGAKNNLNNRIRIASSV
                     YYIKWNNIQQVVVPPICEISFISNLGKAVSKGIDLEVQASLTDSLALDLAAGYNSARY
                     TQDSKLSGTETSPIVANGDAIVGQTAESGYGQATTPVTVAAGLEYRFNLFSRQSFLRV
                     DYTYEGRAKWPTAAQDPNTLQYDPNNYVLPGTTFTTLRGGMQFGSLSLSAFCDNLTNS
                     HAITAYNWSINPGDGNSRLERQYTFRPRTIGITAIYHY"
     gene            complement(1878..2312)
                     /locus_tag="VI195_11185"
     CDS             complement(1878..2312)
                     /locus_tag="VI195_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6824997.1"
                     /translation="MAARLAVLTAALAASFTTVAVCASPAQDYMLYCMGCHGAEARGV
                     PGKIPPLAGTLALFMRIAEGRNYVLRVPGAANSALPDAQLTAVLNWLAMSYGAPGEPP
                     PAPFTVAEVSSVRHTPLADVQARRREVIGMLAASGTAPPAQY"
     gene            complement(2316..2855)
                     /locus_tag="VI195_11190"
     CDS             complement(2316..2855)
                     /locus_tag="VI195_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6824998.1"
                     /translation="MSRARARRTLQTRSTAAFTLPVRIAALACLAAAFVPAPGARAAD
                     QSAYPQGAAAFQTNCALCHGSAGAGVPGLAPPITTYPARYAASPEGRRQLAMTVLYGM
                     FGPISLGEARFNAQMPDFGRLDDATLAATLNFVVFDLAHAAADVKPLTAAEIASERAH
                     PLDGAAVRAHRQSLTTAGS"
     gene            complement(2852..3367)
                     /locus_tag="VI195_11195"
     CDS             complement(2852..3367)
                     /locus_tag="VI195_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HEY6824999.1"
                     /translation="MRTLDQLSERSTRSLAQRTSRRSLLASLGQLLTGAALLPLLPVD
                     RAARARAAETKPQAAGPEDCHYWKYCAIDGFLCSCCGGTSASCPPGTAPSPISWIGTC
                     HNAGDGRDYIVSYNDCCGKSTCGTCDCVRNEGEKPIYRPSRNNDLNWCMANADVNYHC
                     TVSVILGVAEK"
     gene            complement(3385..>3752)
                     /locus_tag="VI195_11200"
     CDS             complement(3385..>3752)
                     /locus_tag="VI195_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HEY6825000.1"
                     /translation="WSLVSASEKGHWRPGGNQLGAIHRELGRLYVPMHEGGEGTHKDG
                     GTEIWVYDMAGHRRLARWPLKVHGLSRVTAVQVSQDPAPILFAATETSDLAVFDALTG
                     RLRHIKKHVAQSPWMLLNP"
BASE COUNT          722 a         1272 c         1148 g          610 t
ORIGIN      
        1 taccggcgcg atggcggctg ggtggacgat atcaacccgg tgacgctcgc acccgtggaa
       61 agcgatgcca actacgtcga caactatctg gcgcggctcg cgttcctgtg gacgccgagc
      121 gacaagtggt cactcacacc ggcgatctac taccaggact ccaagcgcca cgacatcaac
      181 aatttctggc cgctgtactc cgatgcgagc aacggcaagt tcgtcaacgc ggatcccacg
      241 cagcggcgcg tgccggataa gttctatctc gcctcactca aaatccaggg ggacctcggc
      301 gcagcccagt tcatctccaa cacctcctac tacgaccgca ccaaccagga cggttacgag
      361 ggcacgatct acaacctggg cttttatcag atctatgcct tcctcccgcc gtacgtgagc
      421 ggctcctacc cgctgatcga cggcaacggc atccacctgc ctccgggcgc gacgaactac
      481 acctcacctt caactgtcga caacggccag atcaacatca gccaggaatt ccgcctgcag
      541 tcgagcgacc cgaacgcgcg gctgctgtgg accacgggct tgttcttcag cgagaaccgc
      601 cagagctatc tggagcagat tcacgatccc tatctcgatc agttgtcact ggctctcacc
      661 ggattgcatt acaccgacat cttcgtcgat gcgaatggca accccgtgcc gtatgtgcca
      721 ggcttcccca acgattccta cttcctgcag acccatgcca aggatgagca gtacgccatc
      781 ttcggcgaag cgaccttcgc ggccaccgag aagctcaaac tgacggccgg tgcccgcttc
      841 tcgaagaaca agtattcgtt cgacacctac accggcggtc cgcagctgtt tgccgcgccg
      901 accaccggct caggcaacaa ctcggagaac tccttcaccc cgaagctgag cattcagtat
      961 caggccgacc cgaacaacat gttctatgcc acctacgcca aggggttccg gcctggtggc
     1021 gccaacaacc cgctgcccta cgcggcctgc tcacaggact tccaggcatt cggtatttcg
     1081 aacgcgccaa cgacctacaa ctccgacacc gtcgacagct tcgagatcgg cgccaagaac
     1141 aacctcaaca accgcatccg catagcgagc agcgtctact acatcaagtg gaacaacatc
     1201 cagcaggtgg tggtgccgcc gatctgcgag atctccttta tctccaacct cggcaaggcg
     1261 gtctccaagg gcatcgacct cgaggtccag gcatcgctca ccgattccct ggcgctcgat
     1321 ctggcggcgg gctacaactc ggcccgctac acgcaggatt cgaaactgag cggcaccgag
     1381 acgagcccga tcgtcgccaa cggcgatgcc atcgtcggtc agaccgccga gtccggttac
     1441 gggcaggcga ccacacccgt gacggtagca gcgggactgg agtaccgctt caacctgttc
     1501 agccgccaga gcttcctgcg cgtggactac acctatgagg ggcgcgccaa gtggccgacc
     1561 gcagcgcagg atcccaacac gctgcagtac gatccgaaca actacgtgct gccgggaacc
     1621 acctttacca ccctgagggg aggcatgcag ttcggatcgc tgtcgctctc ggcgttctgc
     1681 gacaacctga cgaacagcca cgccatcacc gcttacaact ggtcgatcaa tccgggcgat
     1741 ggcaacagcc gcctcgagcg ccagtacacc ttcaggccgc gcaccatcgg catcacggcg
     1801 atctatcact actaggccgg agcggcgggg taaggcggcc gccagcgcaa gccggcaacg
     1861 cccgcagcgc acccctctca gtactgcgcc ggcggcgccg tgccgctcgc ggccagcatg
     1921 ccgatgacct cgcgccggcg cgcctgcaca tcggcgagtg gcgtgtgccg cacgctggag
     1981 acctcagcga ccgtgaaagg cgcgggcggc ggctcacccg gcgcaccgta gctcatcgcc
     2041 agccagttga gtacggcggt gagctgcgca tcggggagcg ccgagttggc ggcgcccggc
     2101 acgcgcagca cgtagttgcg tccctctgcg atgcgcatga aaagcgccag cgtaccggcg
     2161 agcggtggaa ttttcccggg gacaccccgt gcctcggctc catgacaacc catgcaatag
     2221 agcatgtagt cctgtgcggg gctggcacat accgcaaccg tggtgaagct tgcagccaaa
     2281 gcggcggtga gcaccgcaag acgcgccgcc atgattcagg accccgcggt ggtcaggctc
     2341 tggcgatggg cgcgcaccgc ggcgccatcc agcggatgag cacgctcgct ggcgatctct
     2401 gcggcagtga gcggcttcac gtccgccgct gcgtgcgcca ggtcgaacac cacgaagttg
     2461 agtgttgcgg cgagcgtcgc atcatcgagc cgcccgaagt ccggcatctg cgcattaaac
     2521 cgcgcctcgc caaggctgat cgggccgaac attccgtaca gcaccgtcat ggcgagctgc
     2581 cggcgccctt ccggggacgc cgcataacgg gcgggatacg tcgtgatcgg cggtgcgagc
     2641 ccgggtactc cagcgcccgc agacccgtgg cagagggcgc agttcgtctg gaaagccgcg
     2701 gctccctgtg gataggccga ctggtccgcg gcacgcgcgc cgggcgccgg cacgaaggcg
     2761 gcagcaaggc aggcgagggc ggcaatccgg accgggagcg tgaacgcagc cgtgcttcgt
     2821 gtctgaagcg tccggcgcgc acgagcgcgg ctcatttctc ggcgactccc aggatcacag
     2881 acaccgtgca gtgatagttg acatcggcgt ttgccatgca ccagttgaga tcgttgttgc
     2941 gcgaggggcg gtagattggc ttctcgccct cgttgcgcac gcagtcgcaa gtgccgcagg
     3001 tactcttacc gcaacagtcg ttatacgaca cgatgtagtc gcgaccgtcg ccggcgttgt
     3061 gacaagtccc gatccaggag atgggtgagg gcgccgtccc gggtgggcac gatgcggagg
     3121 tgccgccaca gcaggagcac aggaagccat cgatcgcgca gtatttccag tagtggcagt
     3181 cctcgggacc ggcagcctgc ggcttggttt ccgccgcgcg cgcgcgggcg gcgcgatcca
     3241 cgggcagtaa cggcagcagc gccgcgccgg tgagcagctg cccgagcgac gccagcagac
     3301 tgcggcgcga ggtgcgctgc gccagtgagc gggtcgagcg ttcggacaac tgatcgagtg
     3361 tacgcatcat tggactccgt gccgctacgg attgagcaac atccagggcg actgagcgac
     3421 gtgtttcttg atgtgtcgca gtcggccggt gagtgcatcg aagactgcca gatcagacgt
     3481 ctcggtcgcg gcgaaaagga tcggggcggg atcctgcgac acctgcacgg cggtgacacg
     3541 cgacagcccg tgcaccttga gcggccagcg tgccaggcgt cggtggcccg ccatgtcgta
     3601 cacccatatt tcggtgccgc catccttgtg cgtgccttca ccgccctcgt gcatcggcac
     3661 gtacagacgc ccgagctcgc ggtggatggc acccagctga ttcccgcccg gtcgccaatg
     3721 ccccttctcg gaggcgctca ccagcgacca tg
//
LOCUS       DATDMP010000637         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_211625, whole genome shotgun sequence.
ACCESSION   DATDMP010000637 DATDMP010000000
VERSION     DATDMP010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211625"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2164)
                     /locus_tag="VI195_11205"
     CDS             complement(<1..2164)
                     /locus_tag="VI195_11205"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY6825001.1"
                     /translation="MKGQVEGPLTSLASELQLTSAVSIRGSPRETLEASIKARGLPAL
                     ESATLQAHGQLEGAPLQLDASLDRAAGGALHLLVRRAGWKSAQIEGELTTAANMSAGR
                     GGLRLSMARLADLQPLLGTSLEGSLSGSLALRPVGSRTFAQLSLDARNVVAAGVPADA
                     RLSASGTPDAMDLQLKLQSAKLQGEPANLDTEAHLNLTARELRFDRIDARYHGQSLRL
                     LAPAPVNFADGVAIRNMKLGLQNAVFELDGKVSPLDLHAAAHHVDASVINAFVPDVLA
                     QGTFDVDAKLAGAPSAPTGLVTLNGSQLKVGAARDLQAVDVRATAHLMGASTQLDAHV
                     SGGRTSQLSLTGTAPLDTQGALNLKVNGKLDAGFANPLLEARGERLAGTLTIDATVKG
                     AVRSPDIQGTIDLANGDLRDYVQGAHLSNIAAHLTGSQGTLRIESLTAHAGPGELSVT
                     GQVGLLQPKLPISVRLTAKRAQPVTSDILNANLDADVKVEGTLRERIDVAGTINVNRT
                     VIGIPNALPPQVAVLDVRRPGQAPAPPPERKLVIGLDLTLHAPREILVQGRGLNAELG
                     GTLHIRGTTAEPVVSGGFNMIRGTFSLASTRLNFTNGRVSFSGTGLAGKLDPTLDFTA
                     QATVTDATVTLHITGLADSPQFELSSSPALPQDEILARLLFGESASQLSALQVAQIGA
                     ALASLSGVGGSGPNPLVKVQKSLGLDVLSVGSGSSTTGQ"
BASE COUNT          357 a          740 c          800 g          382 t
ORIGIN      
        1 cttgaccggt tgtgctgctg ccgcttccga ccgacagcac atcgagcccc aaggacttct
       61 gcaccttgac gagcgggttg ggtccacttc cgccgacgcc gctcagggag gcgagggcgg
      121 cgccgatctg cgccacctgc agtgccgaga gttgcgatgc gctctcaccg aagagcagcc
      181 gcgcgagaat ctcatcctgc ggcaaggccg ggctgctgct gagctcgaac tgcggcgagt
      241 cggcgagacc tgtgatgtgc aaggtcacgg tcgcatcggt gacggttgct tgcgcggtga
      301 aatccagtgt cgggtcgagc ttgccggcga gcccggtgcc gctgaagctc acccggccgt
      361 tggtgaagtt gagccgggtg ctggcgagtg agaaggtgcc acggatcata ttgaaaccgc
      421 cgctcaccac cggctcggcc gttgtgccgc ggatatgcag ggtgccgccg agctcggcgt
      481 tcaacccgcg cccctgcacg aggatctcgc gcggggcgtg tagcgtgaga tcgaggccga
      541 tcacgagctt gcgctccggc ggcggggcgg gcgcctgccc ggggcggcgc acatcgagca
      601 ccgccacctg cggtggcagc gcgttgggga tgccgataac ggtgcggttg acgttgatcg
      661 tgcccgcgac atcgatgcgc tcacgcaagg tgccctcgac cttgacgtcg gcatcgagat
      721 tcgcattgag gatgtcgctc gtcaccggct gcgcacgctt tgccgtcagc cgcacggaga
      781 tcggcagctt cggctgcagg agacccacct ggcccgtgac cgagagctcg ccgggtcccg
      841 cgtgcgcggt cagcgactcg attctcaagg tgccctgact ccccgtcagg tgcgccgcaa
      901 tgttggaaag atgcgcaccc tggacgtagt cgcgcagatc tccattggca agatcgatgg
      961 ttccctgaat gtccggcgag cggacggcgc ctttcaccgt cgcatcgatg gtgagggtac
     1021 ccgccaggcg ctcaccgcgc gcttccagca gcggattggc gaaaccggcg tccagcttgc
     1081 cgttgacctt gagattcaag gcgccttgcg tatcgagcgg ggcggtgccg gtgagcgaga
     1141 gctgcgaggt gcggccgccg ctcacgtgcg catcgagctg ggtgctcgcg cccatgagat
     1201 gcgcagtggc gcgcacgtcc accgcctgta ggtcgcgcgc cgccccgacc ttgagctggc
     1261 ttccattcag cgtcacgagg cccgtaggcg ccgaaggcgc tcctgcgagc ttggcatcca
     1321 catcgaaggt gccctgggcg agcacgtcgg gcacgaatgc attgatcacg gaggcatcga
     1381 cgtggtgtgc ggccgcgtgc aggtcgagcg gcgaaacctt accgtcgagc tcgaacacag
     1441 cgttttgcag gccgagcttc atgttgcgga tcgccactcc atcagcaaag ttgaccggcg
     1501 ccggcgccag caggcgcaag gattgaccgt gatagcgcgc gtcgatgcga tcgaagcgca
     1561 gctcgcgggc ggtcaggttg agatgcgctt cggtatcgag gttggcgggc tcgccctgga
     1621 gcttggcgga ttgcagcttg agctgcaggt ccatggcatc gggcgttccc gaggcactca
     1681 agcgcgcatc ggcaggaacc ccagcggcca cgacgttgcg ggcatcgagc gatagctgcg
     1741 caaacgtgcg gctcccgacg ggccgcagtg cgaggctccc tgagaggctg ccctcgaggc
     1801 tcgtgccgag taagggctgc aggtccgcga gacgagccat gctcaagcgc agtccgccac
     1861 gcccggcgct catgttcgcg gcagtagtca gctcgccttc gatttgtgca ctcttccagc
     1921 cggcacggcg gaccagcagg tgcagggcgc cgcccgcagc gcgatcgagc gacgcgtcga
     1981 gctggagcgg tgcaccttcc agttgaccgt gcgcctgcag ggtggcgctc tcgagcgcgg
     2041 gcaagccgcg tgccttgatg ctcgcctcga gcgtttcgcg cggcgaaccg cggatcgaga
     2101 ccgccgatgt cagctgtagc tcgctcgcca gtgatgtgag gggtccctcg acctggccct
     2161 tcaatgtcaa atcgcccgcc agcgccggcg agagtaacgc aaggttcgcg aggcttgccg
     2221 catagcgagc ctgcaccgcc tccagggcgt gcggcgcgcc tgcagctgcg cgccgcgcg
//
LOCUS       DATDMP010000638         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1323142, whole genome shotgun sequence.
ACCESSION   DATDMP010000638 DATDMP010000000
VERSION     DATDMP010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323142"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VI195_11210"
     CDS             complement(<1..697)
                     /locus_tag="VI195_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY6825002.1"
                     /translation="METGLRVLLLTALLATLPGCASMRNWFHHGSQPPSAPSAAAQSP
                     DEAPPRVVEPEVARREIKVPKIRSSNIELGLFYGELSIEDFGAHPVSGITAAYHITED
                     FFFQAEVGRAQAGRTSFEVLGGNIQLLTESERQFTYYDLSLGYNFLPGEAFFGRGVAM
                     TSAFYLLGGFGGTDFAGDTKFTVNFGAGYRVVPVDWLAVHIMAQDHVFQSSLLGTTKL
                     TNNLEARIGVTVFF"
     gene            complement(667..1422)
                     /locus_tag="VI195_11215"
     CDS             complement(667..1422)
                     /locus_tag="VI195_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6825003.1"
                     /translation="MITRARHLALAGLLLLAALCASAPSAAREYLQLFVTEPYLELHT
                     GPGRGYPVFHVVARDASVDVLFRRTDWFKVRTERGVEGWASQADMLKTVLADGSPFRF
                     PLGDRAGFSAHRWEMGIFAGEYGAATYISGYAALSLNSQLQIEAAVGNFLGKFTNGVT
                     GDIGLAHIILPEARLSPFLTLGVGLVHTEPKATLVQPADRTEQSAYVGGGLRYYLTRR
                     FFLRAEYKAHWIFTRRNANEEADEWKLGFAFFY"
     gene            complement(1436..>2297)
                     /locus_tag="VI195_11220"
     CDS             complement(1436..>2297)
                     /locus_tag="VI195_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825004.1"
                     /translation="VNCNGCHRPATAQSPHSHGRSGVFASAWAGAATSGVAFPHTIAS
                     GAGAFIPQAGETMAEARMRVSCLSDIPPCKQMVPNVNVLYSDVWTDPAQATPGTPINL
                     RYDDATQFFTAFPTSALCVTQWAANCRVIINYPQHIQPLWDLPRPNPPVAGVDNTCTQ
                     MGCHNPKDAMGKAQTPAGNLDLTKTPSQAVPQELTSYQQLLFARPTVIMGPNGPIPGP
                     PDPPVMNAGSANGGASAQFFACLTSGAGCMAPSHAGFMTPDELRLVSEWLDIGAQYFN
                     NPFDPAVPVN"
BASE COUNT          397 a          717 c          805 g          378 t
ORIGIN      
        1 aaaagaatac ggtcacgccg atgcgcgcct cgaggttgtt ggtgagtttg gtggtgccga
       61 ggagcgagga ctggaaaacg tggtcctgcg ccatgatgtg cacggccagc caatcgaccg
      121 gcacgacccg gtagcccgcg ccaaagttca ccgtaaactt cgtgtctccg gcgaaatcgg
      181 tgccgccgaa accgccgagg agatagaaag cgctcgtcat ggcgacgccg cgaccgaaaa
      241 aggcctcgcc cggcaggaag ttgtagccga gtgagaggtc gtaatacgtg aattgcctct
      301 cagactcggt aagcaactga atgttaccgc ccaggacctc gaaactggtg cgcccggcct
      361 gggcgcgccc gacttccgcc tggaagaaga agtcctcggt gatgtggtag gccgccgtga
      421 tgcccgagac cggatgagcg ccgaaatcct cgatggacag ctcgccgtag aacaggccga
      481 gctcgatgtt ggagctgcgg atcttgggca ccttgatctc acggcgcgcc acctcgggct
      541 cgaccacgcg cggcggcgcc tcatcgggtg actgggcggc cgcactcggc gcgctcggcg
      601 gctggctgcc gtgatggaac cagttgcgca tcgaggcgca cccgggaagc gtcgcgagca
      661 gcgccgtcag tagaagaacg cgaagcccag tttccattcg tctgcttcct cgtttgcgtt
      721 acgccgcgtg aaaatccagt gcgccttgta ctcggcgcgc aggaagaagc gccgtgtgag
      781 gtagtagcgc aggccgccac cgacataggc ggactgctcg gtgcggtcgg ccggctgtac
      841 cagagtcgcc ttgggctcgg tgtgcaccag tcccacaccg agtgtgagga agggcgacag
      901 ccgggcctcg ggcaggatga tgtgcgcgag gccgatgtcg ccggtcacgc cgttggtgaa
      961 cttgccgagg aagttgccga ccgcagcctc gatctgcagc tgtgagttca gcgacagcgc
     1021 ggcgtacccc gatatgtagg tcgcagcgcc gtactcgccg gcgaagatgc ccatctccca
     1081 gcgatgcgcg ctgaagccgg cgcggtcgcc gagcgggaag cggaacggcg agccgtccgc
     1141 cagcacggtc ttcagcatgt cggcctggct cgcccagcct tctacgccgc gctcggtgcg
     1201 caccttgaac cagtcggtgc ggcggaacag cacatcgacg ctggcatcgc gcgctaccac
     1261 gtggaatacc gggtagcccc gcccggggcc agtgtgcaac tccaggtacg gctcggtgac
     1321 gaacagctgc aggtactcac gtgcggcact cggcgcggag gcgcacagcg ctgccagcag
     1381 caggaggccc gcgagcgcca ggtgccgcgc gcgcgtgatc aattccgtcc tttgctcaat
     1441 tgaccggcac cgccggatcg aaggggttgt tgaagtactg cgcgccgatg tcgagccact
     1501 cggacacgag acgcagctca tcgggagtca tgaagcccgc gtgcgagggt gccatgcagc
     1561 ctgcaccgct ggtgagacag gcgaagaact gcgccgaagc gccgccattc gcgctgccgg
     1621 cattcatgac cggcggatcc ggtggtcccg ggatgggtcc attgggtccc atgataacgg
     1681 ttgggcgcgc gaacagcagc tgctgatagg aggtgagctc ctgcggcact gcctgcgaag
     1741 gcgtcttggt gagatcgagg ttgcccgccg gcgtctgcgc cttgcccatg gcgtccttcg
     1801 ggttgtggca acccatctgc gtgcaggtgt tgtccacgcc ggcaaccggc gggttgggac
     1861 gcggcagatc ccacagcggc tggatgtgct ggggatagtt gatgatgacg cggcagttgg
     1921 ccgcccactg cgtgacgcac agcgcgctgg tcgggaaggc ggtgaagaac tgcgtggcat
     1981 cgtcgtagcg cagattgatc ggtgtgccgg gtgtcgcctg cgccgggtcg gtccacacat
     2041 cgctgtagag cacgttgacg ttcggcacca tctgcttgca gggcggtatg tcgctcaggc
     2101 aactgacgcg catgcgcgcc tcggccatcg tctcgcccgc ctgcggtatg aaggcaccgg
     2161 cgccgctggc aatcgtatgc gggaaggcga ccccgctcgt ggccgcgccg gcccaggcgg
     2221 aggcgaacac accgctgcgc ccgtgcgaat gcggactctg cgcggtggcc ggccggtggc
     2281 agccgttgca gttgacc
//
LOCUS       DATDMP010000639         3875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_323702, whole genome shotgun sequence.
ACCESSION   DATDMP010000639 DATDMP010000000
VERSION     DATDMP010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323702"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1251)
                     /locus_tag="VI195_11225"
     CDS             complement(160..1251)
                     /locus_tag="VI195_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6825005.1"
                     /translation="MRAARYASLLAGAALAAAANASAADPPAPAERPVVEIGTVSVRP
                     MKVESWIPGSIVSRSDARIASIIAGRVVWIAEVGTQVARGEALARLDDTVLRLQLEDL
                     RAQVARARAQQQFNSSQLERFNRLAATQALSASQLEDARAQREMSTDDLTRVEAQLRQ
                     VQYDIDQSVIRAPFPGVVTERFIQRGEYLQTGAATVRLVNTSDIEARATAALGLAGNV
                     HPGQSVTVRDHGIEKSGRVRTVVPVGDDRSRQFEVRVTLASPEWLVGTPIEVSLPSAA
                     VRTAVTVPRDALVIRQHHSYVLRLTRTDTVEELDVTPGAGSADVLEVHGPLAAGDRLG
                     LRGGERLAAGQAVRVVDPTHRAARELHPG"
     gene            complement(1248..3731)
                     /locus_tag="VI195_11230"
     CDS             complement(1248..3731)
                     /locus_tag="VI195_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6825006.1"
                     /translation="MPDIANRAASANNVSGGLLDVGRRQYTLRFTGRFSPEQLGALVL
                     TWRDGRPVHLADVAAIDVAPPPRQFYAYQNGNPAVGIQVLRANGANVLATLDQVKAAV
                     AELREGPLRARGLGIEQSFDASLFIRRAVHLLTENLIVGALLALVVVWWFMREWRVTL
                     LIAATIPVCLCATFVVLDMFGRSLNVISLAGLAFAVGMVVEGAIVVSGNVVRLREEGV
                     PLEEACRNGARQVAGALLASTATTVAVFVPVLFLKDVEGQLFGDLALTISIAVAISIV
                     AALTLLPAALGFALRYPLRPSGYGQGWPALTEWILRMTSTRSRQLGWVVGLLIVPLLL
                     AWWLLPPLDYLPPVKRAAIDSFFDFPPGMSPEGVNRELLPTLLERMKPHMDGRVEPHL
                     KNWYILTWPGGGTLGARVIDEQRIGELEQLVRGSITAGLPDTRVFTVEGDLFGGIGGS
                     VRSVGIHLQSEDSAALNRVAVEGRALLEKVFPGAAVQSFPNPEDVSLELHAVPDDRRI
                     AEVGWDRATLGTVVRAVGEGAWLGEYFDGKSRLPIMLRSSGGATPEDLSNAPLMTPSG
                     LVVRLGDLVQLSTEPGPPAIRRLNHRRAVTLTIDPPPTLSLENVLKIIDAQVLPALRA
                     HMPADGALSLAGSADGLSRTLAAMSRVFVMALIVLLLLMTVLFRSLRDSVIVLLTVPL
                     ALVGGVLGLRILDLVRFQALDLLSMIGFVMMIGVIINHAILLVAAVRSVESEGATLEQ
                     GIRAGLKQRLRAILASTLTGALGALPMAVNPGPGSVIYRGLAAVNIGGVVLSLGFSLV
                     LIPALMRLLAPRCAAVAPGAAALASPAAS"
     gene            3765..3872
                     /locus_tag="VI195_11235"
     CDS             3765..3872
                     /locus_tag="VI195_11235"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY6825007.1"
                     /translation="MLGLIGVVPLVTGIVGFCPLYRLVGLSTCPMPQKK"
BASE COUNT          659 a         1336 c         1327 g          553 t
ORIGIN      
        1 ggtgttctcg gtacgactgc tcggggatga tgtccgacac gcgccacgcc gctgggcgac
       61 ctcgcccgag tttgccggcc tcgaggcgca gctgcctgcc ggtgcgccga tggagcatgc
      121 gctcttcccg ttgctgtggc aggagttatc cgccggcgcc tagcctggat gcagctctcg
      181 cgccgcccga tgggtagggt caaccacgcg cactgcctgg cccgccgcga gccgctcgcc
      241 gccgcgaagc ccgagccgat cgccggcggc cagtggcccg tgtacctcga ggacatccgc
      301 gctacccgct ccgggtgtca catcgagctc ctcgaccgtg tcggtacggg taagccgcag
      361 gacataggag tgatgctggc ggatgacgag cgcatcacgc ggcacggtca cggcggtgcg
      421 cacggcggcg gaaggtagac tcacttcgat gggtgtcccc accagccact cggggctggc
      481 cagtgtcacg cgcacttcga actggcgcga gcgatcatcc ccgactggca cgaccgtgcg
      541 cacgcgtcca cttttctcga tcccatgatc gcgtaccgtg acgctctgtc ccggatgaac
      601 attgccggcg aggccgagcg ccgcggtcgc acgcgcttcg atgtcgctcg tgttgacgag
      661 gcgcacagtg gcggcgcccg tctgcaggta ctcgccgcgc tggatgaagc gttcggtgac
      721 caccccggga aagggagcgc gaatgacgga ctgatcgatg tcgtactgca cctgccgcag
      781 ctgggcctcg actcgcgtca gatcgtcggt cgacatctcg cgctgcgcgc gcgcgtcctc
      841 gagctggctg gcgctcaatg cctgggttgc cgccagacga ttgaagcgct cgagctgtga
      901 gctgttgaac tgctgctgcg cgcgcgcccg cgccacctgc gcgcgcaggt cctcgagctg
      961 cagccgcagc accgtgtcat cgagccgcgc gagcgcctcg ccacgggcga cctgtgtgcc
     1021 cacttccgcg atccacacga cgcgaccggc gatgatgctg gcgatgcgcg catcggagcg
     1081 gctgacgatc gagcccggaa tccagctctc gaccttcatc ggacgtacgc tcaccgtccc
     1141 gatctcgacc acgggccgct ccgccggcgc agggggatct gcggcactgg cgttggccgc
     1201 cgcggcgagg gcagcgccag cgaggagcga ggcgtaacgc gcggctctca tgatgcggcc
     1261 ggtgatgcga gcgcagcggc gcccggcgcg acagccgcgc agcgcggcgc gagcagccgc
     1321 atcagcgccg gtatcagcac cagactgaag ccgagactca gtaccacacc gccgatgttc
     1381 accgccgcga gcccgcggta gatgacgctc cctgggcccg ggttcaccgc catgggcagc
     1441 gcaccgaggg caccggtgag cgtgctggcg agaatggcgc gcaagcgctg cttgagacct
     1501 gcacggatgc cctgctcgag ggttgcaccc tcgctctcaa cgctgcgtac cgcggcgacc
     1561 agcagaatgg cgtgattgat gatcaccccg atcatcatga cgaagccgat catcgaaagc
     1621 agatcgagcg cctggaacct caccagatcg agtatgcgca ggccgagcac cccgcccacc
     1681 agcgccagcg gcacggtgag caggacgatg acactgtcac gcagtgagcg gaacagcacc
     1741 gtcatcagca gcagcagcac gatcagggcc atgacgaaca cccggctcat ggcggcgagc
     1801 gtgcggctga ggccgtcggc gctgccggcg aggctcagcg cgccatcggc tggcatatgc
     1861 gcgcgcagcg ccggcagcac ctgcgcgtcg atgatcttca ggacgttctc gagcgagagc
     1921 gtcggcggcg gatcgatggt gagcgtgacc gcgcgccggt ggttcagtcg gcgaatcgcc
     1981 ggtggtccgg gctcggtcga cagctgcacc agatctccga gccgcacgac aagcccggag
     2041 ggggtcatca atggcgcatt cgagagatcc tcgggcgtcg caccgccgct cgagcgcagc
     2101 atgatcggta gccgcgactt gccatcgaaa tactcaccga gccatgcgcc ctcaccgacg
     2161 gcgcgcacca cggtgccgag cgtcgcgcgg tcccagccga cctccgcgat gcggcggtcg
     2221 tccggcacgg catgcagctc gagcgagaca tcctcggggt tggggaagct ctgtacggcc
     2281 gctcccggaa agaccttctc gagcagcgcg cgaccttcta cggcgacacg gttgagtgcg
     2341 gcggaatcct cgctctgcaa gtgaatgccg accgagcgca cggagccacc gatgccgccg
     2401 aacaggtcgc cttcgacggt gaacacgcgc gtgtccggaa gcccggcggt gatgctcccg
     2461 cgcaccagct gctcgagctc gccgatgcgc tgctcgtcga tgactcgtgc gccgagggtg
     2521 ccgccgccgg gccaggtgag gatgtaccag ttcttcagat gcggctcgac cctgccgtcc
     2581 atgtggggct tcatgcgctc gagcagcgtc ggcagcagct cgcggttgac tccctccggg
     2641 ctcatgcccg gcgggaagtc gaagaacgag tcgatggcgg cgcgcttcac gggcggcagg
     2701 taatcgagcg gcggcagcag ccaccaggcg agcagcagcg gcacgatcag caggcccacg
     2761 acccaaccga gctgccggct gcgcgtgctg gtcattcgca gaatccactc ggtgagcgcg
     2821 ggccagccct ggccgtagcc gctcggtcgc aaagggtagc gcagcgcgaa gccgagcgcg
     2881 gccggcagca gtgtgagcgc ggcgacgatg gagatggcga cggcgatcga gatagtgagc
     2941 gcaagatcac cgaatagctg tccctcgacg tccttcagga acagcaccgg cacgaacacg
     3001 gccaccgtcg tggcggtcga ggcgagcagt gcacccgcga cctggcgcgc cccgttgcga
     3061 caggcctcct cgagcggaac gccttcctcg cgcaggcgca ccacgttccc ggacacgacg
     3121 atcgccccct cgaccaccat gccgacggca aaggcaagac ccgcgagcga gatgacgttg
     3181 aggctgcgcc cgaacatatc cagcaccacg aaggtcgcgc acagacacac cgggatggtc
     3241 gccgcgatca gcagcgtcac gcgccactcg cgcatgaacc accagaccac cacgagcgcg
     3301 agcagcgcac ccacgatgag attctccgtg agcaggtgca cagcgcggcg tatgaagagc
     3361 gaggcgtcga aggactgctc gatgccgagc ccacgtgccc gcagcggacc ctcgcgcagc
     3421 tcggccaccg ccgccttgac ctgatcgagc gtcgccagca cgttcgcacc gttggcgcgc
     3481 aagacttgaa taccgaccgc tggattgccg ttctggtagg cgtagaactg gcgcggtggc
     3541 ggcgcaacgt cgatggcggc cacatccgca agatgcacgg ggcgtccatc gcgccaggtg
     3601 aggaccagcg ccccgagttg ctcgggcgag aaacgtccgg tgaagcgcag cgtgtactgc
     3661 cgtcggccga cgtcgagcag cccgccggaa acgttgttgg cgctcgccgc gcggttggcg
     3721 atgtcgggca cgccgatgcc gagcgcggcg gcggccccaa gagcatgctc gggctcatcg
     3781 gcgtcgtgcc gctcgtcacc gggatcgtgg gtttctgccc gctgtaccgg ctcgtggggc
     3841 tgagcacctg cccgatgccg cagaagaagt gaggt
//
LOCUS       DATDMP010000640         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_989155, whole genome shotgun sequence.
ACCESSION   DATDMP010000640 DATDMP010000000
VERSION     DATDMP010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989155"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..845)
                     /locus_tag="VI195_11240"
     CDS             complement(66..845)
                     /locus_tag="VI195_11240"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEY6825008.1"
                     /translation="MSSSPPVVLSFAASDPTCGAGVQADILTIAAMGCIPVCVVTALT
                     VQDTRGVEGALPVQSNWVEEQARCLLHDLQVSAFKLGVMGSRDNVQVVSRILRDYADI
                     PVVLDPVLASGRGDPLASDDTAQALLELIVPRTTVATPNTLEAARLGGAQRLLELGAR
                     YVLVTGTHARTGEEVVNALHDRGGLVREDRWPRLHGEFHGSGCTLAAAIAAGLGRGHD
                     APQAVREAQEFTWQALAAAHRTGGGQSIPNRFFRVGNPCTS"
     gene            900..1070
                     /locus_tag="VI195_11245"
     CDS             900..1070
                     /locus_tag="VI195_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY6825009.1"
                     /translation="MQYKTWMCLICGWVYDEAAGLPEEGIQPGTRWEDVPMNWTCPEC
                     GARKEDFEMVEI"
     gene            1128..1685
                     /locus_tag="VI195_11250"
     CDS             1128..1685
                     /locus_tag="VI195_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6825010.1"
                     /translation="MSSINLTHHFLIAMPNMADPYFSRTLTYVCEHNEQGALGLVVNR
                     PIDMTLQSLFERLSLKLDGDMADAPIYFGGPVQTDRGFVLHEPAGNWQSTLRVRESIG
                     LTTSKDILEAVGRGEGPRRMLVTLGYAGWSAGQLEHELSQNAWLTVEARDGIIFETPA
                     EERLPAAMELLGLDFARLQDEAGHA"
     gene            1678..2088
                     /gene="ruvX"
                     /locus_tag="VI195_11255"
     CDS             1678..2088
                     /gene="ruvX"
                     /locus_tag="VI195_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY6825011.1"
                     /translation="MPDRAARTVLAFDFGLKRIGVAVGEPELRTAHPLPAIAAAGDAR
                     FAAIEKLVQEWKPARLVVGRPLSTQGEAHLVTRQAERFARRLQGRFRLPVAQVDERYT
                     TVEAESRLRGTRNKRAVDSVAAQLMLEQYFDELA"
     gene            2075..>2305
                     /locus_tag="VI195_11260"
     CDS             2075..>2305
                     /locus_tag="VI195_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825012.1"
                     /translation="MSLPDAEKLCDALAGQLARRVTDKTAMIGIHTGGVWLAERLHQR
                     LGLAVPLGVMDVAFYRDDYHAQGLHHDPKRTRI"
BASE COUNT          412 a          753 c          765 g          375 t
ORIGIN      
        1 agcttcaact ttctggagca gcccgtctga cgcctcgggc gtgatggcgt agagcccgcg
       61 cagcttcagc tcgtgcatgg gttgccgacg cgaaagaaac ggttcgggat cgattggcca
      121 ccgccggtcc gatgcgcggc agcaagcgcc tgccatgtga attcctgcgc ctcgcgcacc
      181 gcttgcggag catcatggcc gcggcccagg cctgcggcga tggcggcggc cagcgtgcat
      241 ccggagccat ggaactcccc gtgcaagcgc ggccagcggt cttcgcgcac gaggccgccg
      301 cggtcatgaa gcgcattcac cacttcttcg ccggtgcgcg catgcgtacc cgtcacgagc
      361 acatagcggg cgccgagctc gagcagccgc tgcgcgccac cgagccgagc cgcttccagg
      421 gtgttcggcg tggcgacggt cgtccgggga acaatcagct cgaggagcgc ctgcgcggtg
      481 tcgtcgcttg caagcggatc acctcgcccg gaggcaagca ccggatcgag caccacgggt
      541 atatcggcgt aatcgcgaag gatccggctg acgacctgca cattgtcgcg ggagcccatc
      601 acgccgagct tgaacgccga gacttgcaag tcgtgaagaa ggcaccgcgc ctgctcctcg
      661 acccagttcg actgaaccgg gagcgcgccc tcaacgccgc gcgtatcctg gacggtgagc
      721 gcggtgacga cgcaaaccgg aatgcagccc atcgccgcga tcgtcaaaat gtcggcctgc
      781 actccagcgc cgcaggttgg gtcggacgcg gcgaaggaaa gaaccacggg cggcgaggaa
      841 gacatgattt agaatgccgg ccgattctac tcgcgcgctt tcccatgact gaaaaccaag
      901 tgcagtacaa aacgtggatg tgcctcattt gcggctgggt ctacgatgaa gcggcgggcc
      961 tgccggagga aggcattcaa cccgggacgc gctgggaaga cgtgcccatg aactggactt
     1021 gtcctgaatg cggcgcgcgc aaggaagatt tcgagatggt ggaaatctaa actttgcgca
     1081 ggcgcagggc tctaaccccc cttgtacagt agaatggggg tggctggatg tcctcgatca
     1141 acctgacgca tcacttcctg atcgccatgc cgaacatggc cgatccgtac ttttcgagga
     1201 cgctcaccta cgtgtgcgag cacaacgaac agggcgcgct cggcctcgtg gtaaatcgac
     1261 cgatcgacat gaccctgcag tcgctcttcg agcggctttc gctcaagctc gatggcgaca
     1321 tggcggacgc gcccatctac ttcggcggac cggtgcagac cgatcgcggc ttcgtgctgc
     1381 acgagcctgc gggcaactgg caatcgacgc tgcgggttcg cgaatcgatc ggcctcacca
     1441 cgtccaagga catcctcgag gcggtagggc ggggcgaggg tccgaggcgc atgctcgtca
     1501 cgctgggcta cgccggatgg tcggccggcc agcttgagca cgagctctcg cagaacgcct
     1561 ggctcaccgt ggaagcgcgc gacggaatca ttttcgagac gccggccgaa gaacggctgc
     1621 ccgccgcgat ggagcttctc ggcctcgatt tcgcccggct gcaggacgaa gcgggacatg
     1681 cctgaccggg cggcgcgcac ggtcctcgcc tttgatttcg gcctcaagcg catcggcgtt
     1741 gcagtcggcg agcccgagct gcgcaccgcg catcccctgc ccgcaatcgc ggccgccggc
     1801 gatgcgcgct ttgcggcgat agaaaagctg gtgcaggaat ggaagccggc gcgcctggtc
     1861 gtggggcggc cgctttccac gcaaggcgag gcgcacctcg tgactcgcca ggcggagcgc
     1921 tttgcgcggc gccttcaagg ccggttccgc ttgcccgtgg cgcaggtcga cgagcgctat
     1981 accaccgtgg aggcggaaag ccgcctgcgt gggacccgga acaagcgcgc ggtcgattcc
     2041 gtcgccgccc aactcatgct cgagcaatat ttcgatgagc ttgcctgacg cggagaagct
     2101 ttgcgacgcg ctcgctggcc agctcgcgcg ccgggtcacc gacaagaccg ccatgatcgg
     2161 catccacacg gggggcgttt ggcttgcgga gcgcctgcac cagcgcctcg gccttgccgt
     2221 gccgctcggc gtgatggacg tcgcgttcta ccgcgacgat taccacgctc agggcctgca
     2281 ccacgatccg aagcggaccc gcatt
//
LOCUS       DATDMP010000641         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2213081, whole genome shotgun sequence.
ACCESSION   DATDMP010000641 DATDMP010000000
VERSION     DATDMP010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213081"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VI195_11265"
     CDS             complement(<1..254)
                     /locus_tag="VI195_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6825013.1"
                     /translation="MSTSIVRLDGALEAEVSEPPAERLIAGTPELQLRNYFTDTSQQF
                     FAGRWSATRGKWRVRYTENELCVMTAGRVTIESTSGERLS"
     gene            complement(355..1098)
                     /locus_tag="VI195_11270"
     CDS             complement(355..1098)
                     /locus_tag="VI195_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6825014.1"
                     /translation="MKTATRRASASALTLGGCLALSAACAGQPADAPGEGSKQADIGV
                     VTGLALGAVAAGPIGAVAGAAAGALLGDHYHRQSQTSAALTAELKRSEAERAQLTHSV
                     AQLDDSLSQARSHGDELATELAHTDQLGLDVSFRTADDSVAAQAMSPLLKLGALAASL
                     PQAQVSVAGFADPRGSDAYNDELSLRRAQNVAAVLASAGVPHERIRIEAHGKGAALAV
                     DGDLDSYALERRVTVRLQLPTPAEVARRD"
     gene            1279..>3402
                     /locus_tag="VI195_11275"
     CDS             1279..>3402
                     /locus_tag="VI195_11275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY6825015.1"
                     /translation="MPQQSAPHRARCALWLAALCLAGIAAAAPPPSAPPAEPAGSVLR
                     ATLANGLRVVIVRNTLAPVVATSVNYLAGSDEAPAGFPGTAHAQEHMMFRGSPGLTAD
                     QLADIGRIVGGDFNANTREDLTQYLFTVPAEDLDVALHIEALRMRAVLDGAAEWDQER
                     GAIEQEVAQDLSEPSYVLFSKLRARMFAGTPYEHDALGTRPSFEKTSAQMLKHFHDTW
                     YAPNNAILVIAGDVEPGATLTLVQQLFGDIPAKKLPAKPAVRLRTVHPDRFTVDTDRP
                     SGTLMIAMRTPGPRDADFAALEVLADVLSNRRFELYGLVPQGRALSAEFGLDPLPQAA
                     LAYAALSFTSGEDPKSLEAAVRTILARVAREGVPAELVAAAKLQERSAAQFQRNSIPE
                     LAAVWSDALALYGLASPDEDLARIEQVTVADVNRVAHQYLDLDHAVIGVMQPRGSGPP
                     VASRGGFGGRESISLGEAHPTALPHWAEKALQRLQVPESTLHPLVSTLPNGLTLIVQT
                     ADVSDTVSVFGHIRNRPETEERPGEEGVAQVLERLLTYGSEHLDRVAFQQALDDIGAR
                     QHAGTDFAMQALAEHFERGVELLADNELHPALPHEALEVIRGQVALGVAARNASPGFL
                     TQRSLRAALYPPDDPSLRISTPETVRALTSEAVRGYYTRVFRPDLTSIVVIGRVDPQR
                     AREVIGRYFGGWQASGPKPSIDLPPV"
BASE COUNT          519 a         1198 c         1143 g          542 t
ORIGIN      
        1 aagctcaggc gctcaccgct cgtgctttcg atggtgaccc ttccggccgt catcacgcac
       61 agctcattct cggtgtagcg cacccgccat ttgccgcggg tcgccgacca gcgtccggcg
      121 aagaattgct ggctggtatc ggtgaaatag ttgcgtaact gcagttcggg ggtcccggcg
      181 atcagccgct cggccggcgg ctcggagacc tccgcctcga gtgcgccatc caggcgaacg
      241 atgctcgtgc tcatggcgat cctgcgtagg taatcgtgcc aggaaatctt agcgcgaaga
      301 tgcgccgcga gcttgtaccc gcgccgggca ccggcctgca accgcacccc cgggctaatc
      361 acggcgcgcg acctcggccg gggtcggcag ctgcagccgc accgtcaccc gccgctcgag
      421 cgcatagctg tcgaggtcac cgtcgacggc cagtgccgcg cccttgccgt gcgcctcgat
      481 gcggatgcgc tcgtgcggga cccctgcgct cgccagcacc gcggcaacat tctgcgcacg
      541 gcgcagcgac agctcatcgt tgtaggcgtc cgagccgcgt ggatcggcga accccgcgac
      601 cgacacctgc gcctgcggca gcgatgccgc gagtgctccg agcttcagca gtggcgacat
      661 cgcctgcgct gccaccgagt catccgcggt gcggaagctg acatcgagcc cgagctgatc
      721 agtgtgtgcc agctcggttg cgagctcatc gccgtgcgac cgtgcctgcg acagcgagtc
      781 atcgagctgt gccacgctat gggtgagctg ggcgcgctca gcctcgctgc gcttcagctc
      841 cgcggtgagt gccgcggagg tctgcgactg gcgatggtaa tgatcgccaa gcagcgcgcc
      901 ggcggcagcg ccggctacgg caccgatcgg tccggccgcg actgcgccga gggcgagtcc
      961 ggtgaccacg ccgatgtccg cttgcttcga gccctcgccg ggtgcgtccg cgggttggcc
     1021 cgcgcaggcc gcggaaagcg ccaggcagcc gccgagcgtt agcgcggacg ccgaggcccg
     1081 ccgtgtcgct gtcttcatga tgtattcctc gcttgagtgt tgggggacgg aaccttcgcc
     1141 acggtgatca gttcagcgca cgcgccggcg ccggcggtgg agcaaaagcg tcagcgccag
     1201 gatgatttgt ggcggaacat ggcaaccgcg ggcgttcgca tatactggcc gcccccaagc
     1261 gcgctaccgg gatttgccat gccgcagcag tcagctccgc accgggcgcg atgcgccctc
     1321 tggcttgccg cgctatgcct cgccggaatc gccgccgccg ctccgccacc tagcgcgccg
     1381 ccggcggagc ccgccggcag cgtgctgcgc gcgacgctcg cgaatgggtt gcgcgtggtg
     1441 atcgtgcgca acaccctggc gccggtggtc gcgacctcgg tcaactacct ggccggatcc
     1501 gatgaggcgc ccgcgggctt tcccggtacg gcgcacgccc aggagcacat gatgttccgc
     1561 ggcagccccg ggctcaccgc ggatcaactt gccgacatcg gtcgcatcgt cggcggcgac
     1621 ttcaacgcca acacccgcga ggacctcacg caatacctgt tcaccgtccc ggccgaggat
     1681 ctcgatgtcg cactgcatat cgaagcgctg cgcatgcgcg cggtgctcga tggcgccgcg
     1741 gaatgggacc aggagcgcgg cgccatcgag caggaggtcg cacaggacct gtcggagccc
     1801 tcatacgtgc tgttttccaa gctgcgcgcg cgcatgttcg ccggcactcc ttatgagcac
     1861 gatgcgctcg gcacccggcc ctccttcgag aagaccagtg cgcagatgct caagcacttc
     1921 cacgacacct ggtacgcacc gaacaacgcc atcctggtga tcgcgggcga tgtcgagccg
     1981 ggagctacgc tcacactcgt gcagcagctg ttcggcgaca tcccggcgaa gaagctgccg
     2041 gcgaagcctg cggtacgcct gcgcaccgtg catccggacc gcttcaccgt cgatacggat
     2101 cgcccgagcg gcacgctcat gatcgccatg cgcacgcctg gtccacgcga tgctgatttt
     2161 gccgcgctcg aggtgctggc ggacgtgctc agcaatcggc gcttcgagct ctacggactc
     2221 gtcccccagg gccgcgcgct gagcgcggag ttcggtctcg atcccttgcc gcaggcggcg
     2281 cttgcctatg ccgcactctc cttcaccagc ggcgaagatc ccaagtcgct cgaggccgcg
     2341 gtgcggacca tcctcgcgcg ggtggcgcgt gagggggtac cggctgagct cgtggccgca
     2401 gccaagctgc aggagcgcag cgcggcgcag ttccagcgca actcgatccc cgagttggcc
     2461 gcggtgtggt cggacgcgct ggcgctgtac ggactcgcct ccccggacga ggatctcgca
     2521 cgcatcgagc aggtgacggt cgccgatgtc aatcgggtcg cgcatcagta cctcgatctc
     2581 gatcatgcgg tgatcggggt aatgcagccg cgcggctcgg ggccgccggt cgcctcgcgc
     2641 ggaggcttcg gcgggcgcga atcgatctca ctcggcgaag cgcatccgac ggcgctgccg
     2701 cactgggcgg agaaggcgct gcagcggctg caggtgccgg aatcgactct gcacccgctg
     2761 gtgagcacgc tgccgaacgg cctcacgctc atcgtgcaga ccgctgatgt gagcgacacc
     2821 gtgagtgtgt tcggtcacat ccgcaaccgc cccgagaccg aggagcgccc gggcgaggaa
     2881 ggtgtcgcgc aggtgctcga gcggctgctc acctatggca gcgagcacct cgaccgcgtc
     2941 gctttccagc aagcgctcga tgacatcggc gcgcgccagc acgccggcac ggactttgcc
     3001 atgcaggcac tcgcggagca cttcgagcgc ggcgtcgagc tgctcgccga caacgagctg
     3061 cacccggcac tgccgcacga ggcgctcgag gtcatccgtg gccaggtagc gctcggtgtc
     3121 gccgcgcgca atgccagtcc gggcttcctg acacagcggt ccttgcgcgc tgcgctctat
     3181 ccgcccgatg acccgagcct gcgcatatcg acgccggaga ccgtgcgcgc gctaaccagc
     3241 gaggccgtgc gcggctacta cacccgcgtg ttccggcccg atctcaccag catcgtcgtc
     3301 atcggcaggg tggatccgca gcgggctcgt gaagtgatcg gccgctattt cggtggctgg
     3361 caggcgagcg gcccgaaacc ctcgatcgat ctgccaccgg tc
//
LOCUS       DATDMP010000642         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_989396, whole genome shotgun sequence.
ACCESSION   DATDMP010000642 DATDMP010000000
VERSION     DATDMP010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989396"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /gene="rplQ"
                     /locus_tag="VI195_11280"
     CDS             complement(<1..272)
                     /gene="rplQ"
                     /locus_tag="VI195_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY6825016.1"
                     /translation="MRHHLSGRQLSRNSAHRHALLRNMSVSLLRHETIRTTLPKAKEL
                     RPFAEKLITLAKRDDDRLHARRLAARHIQDPAVVKKLFDTLGARFAG"
     gene            complement(402..1406)
                     /gene="rpoA"
                     /locus_tag="VI195_11285"
     CDS             complement(402..1406)
                     /gene="rpoA"
                     /locus_tag="VI195_11285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY6825017.1"
                     /translation="MQGSVSEFLRPRVVKVQPVAPRQARVVIEPFERGFGHTLGNALR
                     RVLLSSMPGSAITEVEIDGVLHEYTSIEGVQEDVVDILLNLKSVALRMHARDSAELRL
                     HKKGPGPVTAGDIQADHDIEVVNPELVIANLTKAGELAMTLRVERGRGYRPAASRVAF
                     EEQTRPIGRLQLDASFSPVRRVTYSVDAARVEQRTDLDKLVIDIETNGTIDAEEAIRR
                     AGAILKDQLSVFIDLQGEEEAATKVSEMQFDPILMRPVDELELTVRSANCLKAENIHY
                     IGDLVQRTEVELLRTPNLGKKSLTEIKEVLQSHGLMLGMRLEGWPPAGLKHEETRDII
                     "
     gene            complement(1503..2126)
                     /gene="rpsD"
                     /locus_tag="VI195_11290"
     CDS             complement(1503..2126)
                     /gene="rpsD"
                     /locus_tag="VI195_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY6825018.1"
                     /translation="MARYVGPKCKLARREGTDLFLKSGVKPLENKCKFTVPPGGIKGE
                     RRQRLSDYGLQLREKQKLRRMYGVLERQFGNYYQEAARKAGATGENLLRLLECRLDNV
                     VYRMGFAATRAEARQLVSHKSITVNEKAVTIASYQVKAGDIVQVREKSRKQLRIQSAL
                     NVAQQVGLPEWVEVNDKEFKGVFKSAPGRDDILPDINENLVVELYSK"
     gene            complement(2142..>2237)
                     /gene="rpsK"
                     /locus_tag="VI195_11295"
     CDS             complement(2142..>2237)
                     /gene="rpsK"
                     /locus_tag="VI195_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY6825019.1"
                     /translation="ALNNCGLKITSISDVTPIPHNGCRPPKKRRV"
BASE COUNT          336 a          760 c          718 g          423 t
ORIGIN      
        1 ccggcgaagc gggcgccgag cgtgtcgaac agcttcttca ccaccgccgg gtcctggatg
       61 tggcgcgcgg cgagccggcg ggcgtgcagc cggtcgtcgt cgcgcttggc gagcgtgatc
      121 agcttctcgg cgaagggccg cagctccttc gccttcggca gcgtggtgcg aatggtctcg
      181 tggcgcagca gcgagaccga catgttgcgc aacagcgcat gccggtgcgc gctgttgcgc
      241 gacagctgcc ggccggagag atggtgacgc atagttcctc ttgttgactg gcggaaacgc
      301 ccttcgggcc tttccgcggg ccagccggcc agcgcttacg ccctcgcggg caagaggcgt
      361 gactcttgcc cgcgaacagc gggccggttg tgacaattaa atcaaataat gtcgcgcgtt
      421 tcctcgtgct tgaggcccgc gggcggccag ccctcgagcc gcatgccgag catgaggcca
      481 tggctctgca gcacttcctt gatctcggtg agcgacttct tgcccagatt cggcgtgcgc
      541 agcagctcca cttccgtgcg ctgcaccagg tcgccgatgt agtggatgtt ctcggccttg
      601 aggcagttgg cgctgcgcac ggtgagctcg agctcgtcca ccggccgcat cagtatcgga
      661 tcgaactgca tctccgagac cttggtggcc gcttcctcct cgccctgcag gtcgatgaac
      721 accgacagct ggtccttgag gatggcgccg gcgcggcgga tcgcctcctc ggcgtcgatg
      781 gtgccgttgg tctcgatgtc gatgaccagc ttgtcgagat cggtgcgctg ctcgacgcgc
      841 gccgcatcca ccgagtaggt gacgcgccgt accggcgaga aggaggcatc gagctgcagg
      901 cggccgatgg ggcgcgtctg ctcctcgaag gcgacccgcg atgccgccgg acggtagccg
      961 cggccgcgct ccacgcgcag cgtcatcgcg agctcgcccg ccttggtgag gttggcgatc
     1021 acgagctccg ggttcaccac ttcgatgtcg tgatcggcct ggatgtcccc ggcggttacc
     1081 ggccccgggc ccttcttgtg caggcgcaac tccgcgctgt cgcgcgcgtg catgcgcagc
     1141 gccaccgatt tgaggttgag caggatatcg accacgtcct cctgcactcc ctcgatgctg
     1201 gtgtactcgt ggagcacacc gtcgatctcg acctcggtga tggcgctgcc cggcatcgac
     1261 gacagcagca cgcggcgcag cgcgttgccg agcgtatggc cgaagccacg ctcgaacggc
     1321 tcgatcacca cgcgcgcctg gcgcggggcc accggctgca ccttcaccac acgcggtctc
     1381 aggaactcgc ttacagaacc ctgcattgca ctcaccttgc cggctcgacg accggcgcaa
     1441 aagaaaccta cggcggaccc gctcacatgg gggcaaagcc ccccaaattc aagcggctgc
     1501 gtttacttcg aatacagctc cacgaccagg ttctcgttga tgtcggggag gatgtcatcg
     1561 cgccccggcg ccgacttgaa cactcccttg aactccttgt cgttcacctc gacccactcg
     1621 ggcagcccca cctgctgcgc gacgttgagc gcgctctgga tgcgcagctg cttgcgcgac
     1681 ttctcgcgca cctggacgat gtcgccggcc ttcacctggt aggaggcgat ggtcaccgcc
     1741 ttctcgttga cggtgatcga cttgtgactc accagctggc gcgcctcggc gcgcgtggcg
     1801 gcaaagccca tgcggtacac gacgttgtcc aggcggcact cgagcaggcg cagcaggttc
     1861 tcacccgtcg ccccggcctt gcgcgccgct tcctggtagt agttgccgaa ctgccgctcg
     1921 agcaccccgt acatgcggcg cagcttctgt ttctcgcgca gctgcagtcc gtagtcggac
     1981 aagcgctgac ggcgctcacc cttgatgcca ccgggcggca cggtgaactt gcacttgttt
     2041 tccagcggct tcacgccgct cttgagaaac aggtcggtgc cctcgcgccg ggcgagcttg
     2101 cacttgggac ctacgtaacg cgccatcgtt gcctacctct gttacacgcg ccgcttcttc
     2161 ggcggacggc atccgttgtg cgggatcggc gtcacatccg agatgctggt gatcttcagg
     2221 ccgcagttgt tgagcgc
//
LOCUS       DATDMP010000643         3192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_434925, whole genome shotgun sequence.
ACCESSION   DATDMP010000643 DATDMP010000000
VERSION     DATDMP010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434925"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VI195_11300"
     CDS             complement(<1..678)
                     /locus_tag="VI195_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6825020.1"
                     /translation="MAHQSELIVRDIGAYLHAQEHKSLLRFITCGSVDDGKSTLIGRL
                     LYESKLIFDDQLTQLAADSRRVGTRGGELDFALLVDGLAAEREQGITIDVAYRFFSTE
                     RRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGVLVQTRRHSYLVHLLGIPRV
                     VLAVNKMDLVGHSRETFERIVAQYREFAARLGLADITPIPLSAVHGDNVIARRARMDW
                     YQGPALLE"
     gene            complement(678..1628)
                     /gene="cysD"
                     /locus_tag="VI195_11305"
     CDS             complement(678..1628)
                     /gene="cysD"
                     /locus_tag="VI195_11305"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY6825021.1"
                     /translation="MNRAAPRSEASPPRATPATLTHLQRLEAEGIHILREVVAECERP
                     VMLYSIGKDSSVMLHLAMKAFYPAVPPFPLLHVDTTWKFRAMYEFRDATAKRLGMQLL
                     VHVNQEGLRLGIGPFTHGSQVHTDVMKTQALRQALDQYGFDAAFGGARRDEEKSRAKE
                     RIFSFRTAQHRWDPKQQRPELWTLYNARKRPGESLRVFPLSNWTELDVWQYIWLEQIP
                     IVPLYFAAERPVVERDGALVMVDDERMPLRPDERPQMRKVRFRTLGCYPLTGAIESQA
                     DTLPAIIQEMLLTRTSERQGRVIDHDAAASMERKKQEGYF"
     gene            1815..2945
                     /locus_tag="VI195_11310"
     CDS             1815..2945
                     /locus_tag="VI195_11310"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY6825022.1"
                     /translation="MTAEALMCRCLQGITLTALCVAAVAGQTTPAAAATTMPDTATVA
                     PVAEVATAPPAAEAAPASPAAEAATTPPPSTAPQALPTPAQQLEEVIVQTTEPRYVAP
                     TRRDRIGRIWAPVLIDGKGPYRLVLDTGANRSAITRRAAQSLGGAPAVSNTQVTGFTG
                     TAVVPTLHVDSLEVGDLLIGPSDLPVLSDVFGGAQGVLGIEGLANKRIYADFRHDHLE
                     ITLSHGQRAPRDFIVVPLTVTSGGLLVAHVRVGSVRAIAIVDTGAQGTIGNLALRHAL
                     MRVPPRNAAPEEIIGVTLDVQTGDNLLAPDIDFGNLQVKGAHITFGDMYLFQHWKLTN
                     EPTLTLGMDLLGSFDVLVIDYNRHELQIRSRQMPELPYSLRY"
     gene            complement(2996..>3192)
                     /locus_tag="VI195_11315"
     CDS             complement(2996..>3192)
                     /locus_tag="VI195_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825023.1"
                     /translation="RPLARLRALLQTDPAVAIAHATALRALQALPAEPANQSERLHAR
                     HQWNERRRFLRMGPSGRGAS"
BASE COUNT          526 a         1080 c         1059 g          527 t
ORIGIN      
        1 ctcaagcagc gccggtccct gataccagtc catgcgcgcg cggcgcgcga tgacgttgtc
       61 gccgtgcacc gccgagagcg gaatgggggt gatgtcggcg agcccgagcc gcgcggcgaa
      121 ctcgcggtac tgcgccacga tgcgctcgaa ggtttcacgg gagtggccga cgaggtccat
      181 cttgttgacc gcgagcacga cgcgcggaat gccgaggaga tgcacgaggt agctgtggcg
      241 tcgcgtctgt acgagcacgc ccttgcgcgc atcgaccagg atcaccgcca gatccgcggt
      301 ggaggcgcca gtcaccatgt tgcgcgtgta ctgctcgtgc cccggcgtgt ccgcgacgat
      361 gaacttgcgc cgctcggtcg agaagaagcg gtaagcgacg tcgatggtga tgccctgctc
      421 gcgctcggcg gccagcccat cgaccagcag cgcgaagtcg agctcgccgc cgcgcgtgcc
      481 gacgcgcctg gaatcggcgg cgagctgggt caactggtca tcgaagatca gcttcgattc
      541 gtacagcagc cgtccgatga gcgtcgactt gccatcatcc accgagccgc aggtgatgaa
      601 gcgcagcagg ctcttgtgct cctgcgcgtg gagataggcg ccgatgtcgc gcacgatgag
      661 ctccgactgg tgcgccatta gaaataccct tcctgcttct tgcgctccat ggaggcggcg
      721 gcgtcatggt cgatcacccg cccctgacgc tcggaggtgc gcgtcagcag catctcctgg
      781 atgatggcgg gcagcgtgtc agcctggctc tcgatggcac cggtgagcgg gtagcagccg
      841 agcgtgcgaa agcgcacctt cctcatctgc ggccgctcgt ccggccgcag tggcatgcgt
      901 tcgtcgtcca ccatgacgag cgcgccgtca cgctcgacca ccggacgctc ggcggcgaag
      961 tacagcggca cgatcgggat ctgctcgagc cagatgtact gccatacatc gagctcggtc
     1021 cagttcgaga gcgggaatac ccgcagactc tcccccgggc gcttgcgcgc gttgtacagc
     1081 gtccacagct cggggcgctg ctgtttaggg tcccagcggt gctgggcggt gcggaaggag
     1141 aagatgcgtt ccttggcacg gctcttctcc tcgtcgcggc gcgcgccgcc gaaggcggca
     1201 tcgaagccgt actggtcgag cgcctgccgc agcgcctggg tcttcatgac atcggtgtgc
     1261 acctgcgagc cgtgcgtgaa cggcccgatg ccgagccgca gcccctcctg attcacgtgc
     1321 accagcagct gcatgccgag gcgcttcgcc gtggcgtccc ggaactcgta catggcacgg
     1381 aatttccagg tggtgtccac gtgcagcagc gggaacgggg gcaccgccgg gtagaaggcc
     1441 ttcattgcca ggtgcagcat caccgagctg tccttcccga tcgaatagag catcaccggt
     1501 cgctcacatt ccgccaccac ctcgcgcaga atgtggatac cctcggcctc gagccgttgc
     1561 aggtgggtga gcgttgcagg cgtcgcccga ggaggcgagg cttcactacg cggggcggct
     1621 cgattcatgc agcggatact ccggacggct ttgatttatc tggcgtaagt taccatcaac
     1681 gcacctaccg gatgccggaa gtcgaacttg ccgctccgct tggggtcaaa ccggatgcgc
     1741 cgatgggatc ggcggcagga aaactgcgac gaagtaactg gagacctccc ggccggcgtg
     1801 gtctactgtt aaatgtgacc gccgaagcgc tcatgtgccg ctgtctgcag ggaattaccc
     1861 tgacggctct gtgcgtcgct gcggtggcgg ggcagacgac gcccgcggcg gctgccacta
     1921 ccatgcccga caccgctacg gtggcgccgg tagccgaggt cgctacggca ccgccggccg
     1981 ccgaagccgc cccggcatcg cccgccgcgg aagccgctac gacgccgccc ccgtccactg
     2041 cgccgcaggc cttgcccact ccggcgcagc agctcgaaga agtcatcgtg caaaccaccg
     2101 agccccgtta tgtcgcgccg acacggcgcg atcggatcgg gcgcatatgg gcgccggtgc
     2161 tgatcgatgg caagggacct tatcggctcg tgctcgatac cggtgccaac cgctcggcca
     2221 tcacccggcg cgcggcgcag agtctcggcg gagcgcccgc ggtctcgaac acccaggtga
     2281 ccggattcac cggcaccgcg gtcgtaccca cgctgcacgt cgacagcctg gaagtgggcg
     2341 acctgctcat cggaccgagc gatctgccag tgctctcgga cgtgttcggc ggcgcgcagg
     2401 gcgtgctcgg catcgagggt ctcgcgaaca agcgcatcta tgccgacttc aggcatgatc
     2461 acctcgagat caccctctcg catgggcagc gcgcgccacg cgacttcatc gtcgtaccgc
     2521 tcacagtgac cagcggcggc ctgctggtcg cgcacgtgcg cgtcggcagc gtacgcgcca
     2581 tagcgatcgt cgataccggt gcccagggga ccataggtaa cctggcgctg cgccatgcgc
     2641 tcatgcgcgt gccgccgcgc aacgccgcac ccgaggagat catcggcgtc acgctcgatg
     2701 tgcagaccgg cgacaatctg ctcgctcccg acatcgactt cggcaacctg caggtgaagg
     2761 gcgcgcacat caccttcggc gacatgtatc tcttccagca ttggaagctc accaatgagc
     2821 cgacgttgac gctcggcatg gatctgcttg ggtccttcga cgtgctggtg atcgactaca
     2881 accggcacga gctgcagatc cgctcccggc agatgccgga gctgccctac tcgctgcgct
     2941 actgagaggc gagcgcgctg cgctcgtcag ttatcgagcg gccatcgccc gcacctcagg
     3001 atgccccgcg gccactcggc cccatacgca ggaatcgccg ccgctcattc cactggtggc
     3061 gggcgtgcag ccgctcggac tggttggcgg gctcggccgg taacgcctgc agcgcgcgca
     3121 gcgccgtggc gtgcgcgatc gcgaccgccg ggtcggtctg cagcagcgcg cgaaggcgcg
     3181 caagcggtcg ca
//
LOCUS       DATDMP010000644         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_545950, whole genome shotgun sequence.
ACCESSION   DATDMP010000644 DATDMP010000000
VERSION     DATDMP010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545950"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..499)
                     /locus_tag="VI195_11320"
     CDS             complement(11..499)
                     /locus_tag="VI195_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6825024.1"
                     /translation="MSGPDLQQQLVALLPRLRRFARALARDAHDADDLVQLALERALT
                     HAHQVRPDAELGGWMFGIVRNAWIDELRSRGRRARLFVPAELAEHMGGGESQPAHAEL
                     LAVEEALARLPEEQRSAVALVLIEGLSYKEAAHVMQVPIGTLTSRLARGREALQEMLG
                     HN"
     gene            587..1909
                     /locus_tag="VI195_11325"
     CDS             587..1909
                     /locus_tag="VI195_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY6825025.1"
                     /translation="MRRLALIVCLALVRPAGAQVGLPPVRLPQLPPVAVPSLPAALPA
                     NASGDVSVGELDPRRLRELRARRVRELLRRHQDVLEADPQGAPIVRGEVLALTPSPAA
                     MQAAIAAGLSFKRELALSALDLRITVLHVPGATTRALQRLQEQDPSGVYDFNHIYLEA
                     GAVGGTSSASGRAPSAEAADAGSNSGIKVGLIDSGVDEGHEVFRGLTLERHGCAKHAV
                     PAAHGTAIASLLVGRAGAFHGAAPGAALYAADVFCGQPTGGSVEAVAEALAWLVEQRI
                     PVINVSLVGPPDQLLERVVREALARGSLVVAAVGNDGPAAPPLYPAAWPGVIGVTGVD
                     AHGRVLAEAERGTQVKFAAPGADMVAANVRGGYLHVRGTSFAAPIVAGLLALVLPSPD
                     SQRATEAVAVLARSAQHPTSAAVDSAYGYGLVGGELRLQAGLGSARSD"
BASE COUNT          297 a          734 c          683 g          358 t
ORIGIN      
        1 ccgtgcaccc ttagttgtgt ccgagcatct cctgcaacgc ctcgcgaccg cgtgccaggc
       61 gactggtgag ggtcccgatc ggcacctgca tgacgtgcgc ggcctctttg tacgagagcc
      121 cttctatgag caccagcgcc accgcgctgc gttgctcttc cggcagccgc gcaagggcct
      181 cctccaccgc cagcagctct gcatgcgcgg gctgtgattc accaccgccc atatgctcgg
      241 ccagttcagc gggcacgaac agccgtgcac gccgtccccg cgaccgcagc tcatcgatcc
      301 aggcgttgcg cacgatgccg aacatccagc cgccgagctc cgcgtctggg cgaacctgat
      361 gcgcgtgcgt cagcgcccgc tcgagcgcca gctgcaccag atcgtcggca tcgtgagcat
      421 cgcgcgcgag cgcacgcgca aagcgccgca gacgtggcag cagcgcgacg agttgttgct
      481 gcagatccgg tccgctcaat ctgtctcctg ttgccgcact acgtccgagc tcgccatttt
      541 cttccattcc ttcccttccg cgccgacggt gcactaaggt gccggcatgc gccgcttagc
      601 tctcatagtg tgcctggctc tcgtgcgacc tgcgggcgcg caggtcgggc tgccgccggt
      661 gcgcctgccg cagttacctc cggtcgcggt gcccagcttg ccggcggctc tgccggctaa
      721 tgccagcggc gatgtgtctg tgggcgagct cgatccgcgg cgactgcgcg agctgcgtgc
      781 ccggcgtgtc cgggagctgc tgcgccgcca ccaagacgtg ctagaagccg acccccaggg
      841 cgccccgatc gtgcgcggcg aggtgctggc actgacgcca tcgccggcgg cgatgcaggc
      901 cgccatcgcc gcggggcttt ccttcaagcg cgagctggct ctttctgcgc tcgatctgcg
      961 catcaccgtc ctccacgtcc ctggcgcaac gacgcgtgcc ctgcagcggc tgcaggagca
     1021 ggacccttcc ggagtctacg acttcaacca catctatctc gaagcgggcg ctgtaggcgg
     1081 cacatcttcc gcgtccgggc gtgcaccgtc cgctgaggct gccgacgccg gttcgaactc
     1141 cggcatcaag gtcggtctga tcgacagcgg tgtcgacgaa gggcacgagg tgttccgcgg
     1201 cctcacgctg gagcgacacg gttgcgccaa gcacgcggtg ccggcggcgc acggtaccgc
     1261 gatagcctcc cttctcgtcg gacgcgccgg cgcgttccac ggcgctgccc ctggcgctgc
     1321 gctgtatgcc gccgatgtgt tttgcggcca gccgaccggc ggatcggtcg aggcggtcgc
     1381 cgaggcactc gcctggctgg tagagcagcg cattcctgtc atcaatgtga gcctggtggg
     1441 tccgcccgac cagctgctcg agcgtgtggt gcgggaggcc ctcgcacgcg gcagtctcgt
     1501 agtcgctgcg gtgggcaacg acggcccggc agctccgccg ctctatccgg ccgcctggcc
     1561 gggcgtgatc ggcgtgaccg gcgtcgatgc gcacggccgc gtgctcgccg aagccgagcg
     1621 cggcacgcag gtgaaatttg ccgccccggg ggcggacatg gtggctgcca acgtgcgagg
     1681 cggctacctg cacgtgcgcg gcacctcgtt cgcagctccg atcgttgcgg gactgttggc
     1741 actcgtgctg ccttcacccg acagccagcg agcgaccgag gcggtcgccg ttctcgcccg
     1801 ctcggcgcag caccccactt cagctgccgt cgattccgct tacggctacg gactggtcgg
     1861 tggagagcta cgcctacagg cggggctcgg gagcgcccga tccgattaag taaaaagcca
     1921 taacagcaca cactgcggga agaattcgca cgccccactc gtagtactcc gtgaaaggtc
     1981 gcggagtggc tgcggcctcg agaggagacg accatgaaga cgaatcatct tttcgcgagt
     2041 gccgcggttg ctgccgtgtt gctcatcacg gc
//
LOCUS       DATDMP010000645         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_324553, whole genome shotgun sequence.
ACCESSION   DATDMP010000645 DATDMP010000000
VERSION     DATDMP010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324553"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VI195_11330"
     CDS             complement(<1..1160)
                     /locus_tag="VI195_11330"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEY6825026.1"
                     /translation="MIRKPRVPGSSRGAPKVVLAFVIALGEALTGCADKPPPPPPQSG
                     ASSSAQKTAANPQTQAPAQSQAAAQNQAPAQNQTSQIPPDQLDSLVAPIALYPDPLLA
                     QVLAASTYPLEIIQLQQWLAKNKNLKDKALADAVAKQPWDPSVQSMAAMPDLVKRLAD
                     DIQWTTDLGNAFLAQQKDVMDAVQRMRKKAQDKGTLASNEQQKVQTQTVETGQQVIVI
                     EQANPQVVYVPSYDPVVVYGPPVYPYPPIYYPYYPPGAAFVSFSFGFAMGAWMSGGWG
                     YGCGWGTTNVYVNHNNNFVGGAGGVGGVGGVGGVGGVGGVGGVGGVGGVGGVGGAGGV
                     GGAGGVGGAGGVGGVGGPGGAGGVGGPGGAGGGTKPGAGSPKGPGGAGGTSA"
     gene            complement(1413..2174)
                     /locus_tag="VI195_11335"
     CDS             complement(1413..2174)
                     /locus_tag="VI195_11335"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6825027.1"
                     /translation="MNVHAIRAIYHFEMARTFRTLMQSIASPVLSTALYFVVFGAAIG
                     ARMGTVDGVRYGAFIVPGLIMLSILSESVSNASFGIYLPKWSGTIFELLSAPVSYLEA
                     LIGYVGAAATKSVMLGILILVTARLCVPYHIAHPLWMATFLILTAVTFSLFGFIIGLL
                     AESFEQLQIVPLMILTPLTFLGGAFYSISMLPPVWQKITLFNPVVYLISGFRWSFFGV
                     ADVNVLTSAAATLGFLALCVAVVSWIFRTGYKLKS"
     gene            complement(2171..3112)
                     /locus_tag="VI195_11340"
     CDS             complement(2171..3112)
                     /locus_tag="VI195_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6825028.1"
                     /translation="MPESKAPPAVVVSGLRKTYAGGLTALKDIELTIYRGEIFALLGP
                     NGAGKTTLINIICGIVTPSAGTVAANGYDIVRDYRAARSRIGLVPQELHTDSFESVWA
                     TVTFSRGLFGKPPNAAVCEKVLRDLSLWDKKDAKIMTLSGGMKRRVMIAKALAHEPAI
                     LFLDEPTAGVDVELRREMWQLVRGLRDSGVTIILTTHYIEEAEEMADRIGVINHGELI
                     VVEDKMRLMRKLGKRQLTLQLQAPLAQLPQGLNEYPLELAQGGHALVFHFDAHRLETG
                     IPGLLRRLGELGIEIKDLHSQESSLEDIFVGLVSARA"
     gene            complement(3128..>3452)
                     /locus_tag="VI195_11345"
     CDS             complement(3128..>3452)
                     /locus_tag="VI195_11345"
                     /inference="COORDINATES: protein motif:HMM:NF018531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY6825029.1"
                     /translation="IDDAAVARLYEATRQQLQLWTGRLRALAAGQFPDKVTAFRKDMS
                     VHGRFRLPCPVCGTKVQRIRYADNETNYCARCQTAGRLLADRSLSRLLKEDWPREVDA
                     LDREG"
BASE COUNT          596 a         1151 c         1095 g          610 t
ORIGIN      
        1 caggcactgg tcccgcccgc tcctccgggc cccttggggc tgcccgctcc cggcttggtg
       61 cctcctccgg caccgccggg tccaccgacg ccgccggcgc cgccaggacc acccactcca
      121 ccaacccctc cagcaccacc gaccccgccg gctccgccaa ctcctccggc accaccgacc
      181 ccgccgacgc cgccaactcc tccgacaccc cccaccccac cgactccgcc tactccgcct
      241 actccgccga cgccgccgac accgccggct ccgccaacga agttattgtt gtggttcacg
      301 taaacgttgg tcgttcccca tccgcagccg tagccccagc cgccactcat ccacgccccc
      361 atcgcgaacc cgaagctgaa ggacacaaac gcggcgccgg ggggatagta ggggtagtag
      421 atcggtggat aggggtacac cggcggaccg taaaccacta ccggatcgta agaaggcacg
      481 tagacgacct gcggattcgc ctgctcgatc acgatcacct gctggccggt ctcgacggtc
      541 tgcgtctgca ccttctgctg ctcgttcgag gcgagggtgc ccttgtcctg agccttcttg
      601 cgcatgcgtt gcacggcatc catcacgtcc ttctgctgcg caaggaaggc gttgccaaga
      661 tcggtcgtcc actgaatgtc gtccgccaga cgtttcacca agtcaggcat cgccgccatc
      721 gactgcacgc tcgggtccca cggttgcttg gcgacggcat ctgcgagcgc cttgtccttc
      781 aggttcttgt tcttggctag ccattgctgc agttgaatga tctcgagcgg ataagtggag
      841 gctgccagca cctgcgcgag caatggatcg gggtagagcg ctatgggggc gacgagcgag
      901 tcgagctggt cggggggaat ctggctcgtc tggttctgtg ccggggcctg attctgtgcg
      961 gcggcctggc tctgggccgg tgcctgggtc tgtgggttcg cggccgtctt ctgcgcagag
     1021 gaggatgctc cgctttgtgg tggtggcggc ggtggtttat ctgcgcagcc tgtcagggcc
     1081 tcgccgagtg cgatcacaaa cgcgagaacc accttcggcg caccgcggct gcttccggga
     1141 acgcgaggtt tgcgaatcat gggctgcccc cgacctatgt tcggaacgct gaatgcgccg
     1201 gccgcaaggg cggacggccc tgacaacgcc ggactgcaat tgggcctatc gcgcgccgcc
     1261 atggcaagct gccctttcgg gcagtctcat tccagtgggc cggtatgccg gaagcaccgt
     1321 gccggccggg agatcggagc ctccacgggc atctcgccgt attgcgaggc ggcccgtact
     1381 gccggccggg caggcggccg cagcgctccc tttcagctct tgagcttgta accagttctg
     1441 aaaatccagc tcaccaccgc gacacacagg gcgagaaagc cgagcgtcgc cgcggcgctc
     1501 gtgaggacat tcacgtcggc caccccgaag aaactccagc ggaaaccgct gatcaagtac
     1561 acgacggggt tgaagagcgt gatcttctgc cacaccggcg gcagcatgct gatcgagtaa
     1621 aacgctccgc cgaggaaggt gagcggtgtg aggatcatga gcgggacgat ctgcagctgc
     1681 tcgaagctct ccgccaacag gccgatgatg aagccgaaca ggctgaaggt cactgcagtg
     1741 aggatgagga aagtcgccat ccatagcgga tgcgcgatgt gatacggcac gcacagccgt
     1801 gcggtgacga ggatgagaat gccgagcatg accgacttgg tagcggccgc gcctacgtag
     1861 ccgatcaacg cctccagata cgagacgggg gccgacagca gctcgaagat ggtgccggac
     1921 catttaggca gatagatgcc gaatgaggcg ttggatacgc tttcactgag tatcgacagc
     1981 atgatcagtc ccggcacgat gaacgcgccg tagcggacgc catcgacggt tcccatgcgc
     2041 gccccgatcg cggcaccgaa caccacgaag tagagcgcgg tcgagagcac cggggaggcg
     2101 atgctctgca tcagcgtacg gaaggtacgc gccatctcga aatggtagat cgcccggatc
     2161 gcgtgtacgt tcatgcgcgc gcactcacca ggccgacgaa gatgtcctcg agcgagctct
     2221 cctgcgaatg caggtccttg atctcgatgc cgagctcgcc gagccgcctg agcagtcccg
     2281 gaatgccggt ctcgaggcgg tgcgcgtcga agtgaaacac cagcgcatgc ccaccctgcg
     2341 ccagctcgag cggatactcg ttgagtccct gcggcagctg cgcgagtggg gcttgcagct
     2401 gcagggtcaa ctgccgcttg ccgagcttgc gcatgagtct catcttgtcc tcgacgacga
     2461 tcagctcgcc gtgattgatc acgccgatcc tgtccgccat ctcctcggcc tcttcgatgt
     2521 agtgtgtcgt caggatgatg gtgacaccgc tgtcgcgcag cccgcgcacc agctgccaca
     2581 tctcgcggcg cagttcgaca tcgaccccgg cggtgggctc gtcgagaaag agaatcgcag
     2641 gctcgtgcgc cagcgccttg gcgatcatga cgcggcgctt catcccgccc gacagcgtca
     2701 tgatcttcgc gtctttcttg tcccacaggg acagatcgcg cagtactttc tcgcacacag
     2761 ccgcgttcgg cggcttgccg aacagcccgc ggctgaaggt gaccgtcgcc cagacgctct
     2821 cgaacgagtc ggtgtgcagc tcctggggca cgaggccgat gcggctgcgg gccgcgcggt
     2881 aatcgcgcac gatgtcgtag ccgttggcgg ccaccgtacc ggcgctcggt gtcacgatgc
     2941 cgcagatgat gttgatgagc gtcgtcttgc ctgcgccgtt gggcccgagc aaggcgaaga
     3001 tctcgccgcg gtaaatcgtc agctcgatgt ccttgagggc ggtgaggccg ccggcgtagg
     3061 tcttgcgcag accgctcact acgacggccg gtggggcttt gctctcaggc atagcgcggt
     3121 cgcgtcctca tccctcgcgg tccaaggcat ccacttccct cggccaatcc tctttcagca
     3181 gtcgcgagag ggatcgatcc gccagcagcc gcccggctgt ctggcagcgc gcgcagtagt
     3241 tggtctcgtt gtcggcgtac cgaatgcgct gcacctttgt gccgcacacc gggcagggaa
     3301 gccggaagcg gccgtgcacg ctcatgtcct tgcggaacgc ggtgacctta tcggggaact
     3361 ggccggccgc cagcgcgcgc agccgcccgg tccagagctg caactgctgg cgcgtcgctt
     3421 catacagccg cgcgaccgcg gcatcatcga tg
//
LOCUS       DATDMP010000646         2904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1768516, whole genome shotgun sequence.
ACCESSION   DATDMP010000646 DATDMP010000000
VERSION     DATDMP010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768516"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VI195_11350"
     CDS             complement(<1..751)
                     /locus_tag="VI195_11350"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY6825030.1"
                     /translation="MQAQEPQRQARSSNGREPRGETHLDQRRLLRALQAVREGDFSVR
                     LPTDHTGLAGKIADTFNEIVTSNQRLAHELARAGQIVGKDGQTRHRMSSDRRSGAWGA
                     MESSVNTLIDDLLWPTTEVTRTISAVAKGDLQQTMSLQVDGRPLKGEFLRSATVVNAV
                     IEQMSAFTSEVTRVAREVGTEGKLGGQAAVPGVSGTWKDLTDSVNSMASNLTAQVRNI
                     SEVTIAVANGDLSRKITVDVRGEILQLKEAIN"
     gene            complement(1079..1972)
                     /locus_tag="VI195_11355"
     CDS             complement(1079..1972)
                     /locus_tag="VI195_11355"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY6825031.1"
                     /translation="MSHRFEVSHGTCGARGMRDILQRQLETAVAELPRSAASDEEIHR
                     ARKQMKAARATLRLLRPLLSELAWGRGNRALRDAARALSAARDDAVLLQTLGELAGRS
                     KGAGQRRRLGEFQARLQRAAGRRRRELAHHGLRKSAAGLRSALTLLDSWPTPPEDWQP
                     VYRSLRDTYRKGRRCARSNARHASGPTLHEWRKRAKCLRNQLELIAPLQHASVAAMSA
                     KLHELSDHLGEEHDLAVLADLAQRNGRQLGRKTDCALQKVIEKRRRKMRKRAFAVGLP
                     IYAEKPGHFELRLRHYFRRWS"
     gene            complement(1969..2514)
                     /locus_tag="VI195_11360"
     CDS             complement(1969..2514)
                     /locus_tag="VI195_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY6825032.1"
                     /translation="MPQHIPPRESGSGRVHVVIDTPAGSRNKYKYDQQFASFRVSRIL
                     PPGAVFPYDFGSIPGTEAEDGDALDVLVLQLPASFPGCLVTVRLIGVLHAEQRDGSRL
                     IRNDRLIGVVDTPVNPARLQELSALDEEELRAIEHFFKSYNAFQGREFSISSRGGPEE
                     ARRALEGAIAAHGAHSTGKAR"
     gene            complement(2543..>2904)
                     /locus_tag="VI195_11365"
     CDS             complement(2543..>2904)
                     /locus_tag="VI195_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY6825033.1"
                     /translation="PLCSPRADLARRELRAWDPIERHVLWSVHEALPISSGALVTAGG
                     LVFYGTGDGWLKALDARTGRVLWKHRVDGGRKLDAPVSYRGADGHQYIAVRTLAPVVA
                     DTPHEGSATVLLFALAH"
BASE COUNT          439 a          978 c          981 g          506 t
ORIGIN      
        1 ggttgatggc ctctttcagc tgcaggatct cgccgcgcac atcgaccgtg atcttgcgcg
       61 acaggtcgcc gttggcgacg gcgatggtga cctcggagat gttgcgtacc tgcgcggtga
      121 ggttgctcgc catcgagttc acgctgtcgg tcaggtcctt ccacgtgccc gatactccgg
      181 gcacggcggc ctgtccgccg agcttgcctt cggtaccgac ctcgcgcgcg acgcgcgtca
      241 cctcggaggt gaatgcgctc atctgttcga tgaccgcatt caccacggtc gccgagcgca
      301 gaaactcgcc cttcagcgga cgtccgtcga cctgcaggct catggtctgc tgcaggtctc
      361 ccttggcgac cgccgagatg gtgcgcgtca cctcggtggt cggccacaag aggtcatcga
      421 tgagtgtgtt gaccgagctc tccatcgcgc cccaggcgcc cgagcggcgg tcgctcgaca
      481 tgcgatgccg tgtttgtccg tccttgccga cgatctgtcc ggcgcgcgct agttcgtgcg
      541 cgaggcgctg attggaggtg acgatctcgt tgaaggtgtc ggcgatcttg cccgcgagcc
      601 cggtgtgatc ggtcggcagc cgcacggaga aatcgccttc gcgcaccgcc tgtagcgcgc
      661 gcaacagcct gcgctgatcg aggtgggtct ctccgcgcgg ctcgcggcca ttcgagctac
      721 gggcctggcg ctggggttcc tgagcttgca tcagcagcct ccctggggca tgcgcacgcg
      781 gaacagctcc gcatcctcaa tcgatcggtg atgggcgatg ggaaagaaag ggcctgctaa
      841 ccggctgcgc tggcgagcag tgccagcgtg catgcggcaa gtgcgggcgg ttcaattcgg
      901 gcacgatccc ccccggagca gaccgcgcgc aaacagcatc gtgagcgctg cgggtctcct
      961 gaaacgcaca ttaacgcggg cgccgagtgg cgagtgtcag cggaggaggg cagccgccgt
     1021 aggaaataga ctacagtcgg actccgcgcg ttgagttgcg caccggcgat tcagcgcttc
     1081 aggaccagcg tcgaaagtaa tgccgcagcc gtagctcgaa gtgcccgggc ttttccgcgt
     1141 agatcggcag accgaccgcg aaggcacgtt tgcgcatttt gcggcgtcgc ttttcgatga
     1201 ccttctgcag ggcgcagtcg gtcttgcgcc ccaattgccg gccgttgcgc tgcgccaggt
     1261 cagcgagcac cgccagatcg tgctcctcgc caagatgatc ggacagctcg tgcagcttcg
     1321 cagacatggc cgccaccgag gcgtgctgca gcggcgcgat gagctcgagc tgattgcgca
     1381 ggcatttggc gcgcttgcgc cattcgtgca gcgtcggccc gctggcatgg cgcgcgttag
     1441 agcgcgcgca gcgccgccct ttgcggtagg tgtcgcgcag gctgcgatac accggttgcc
     1501 agtcctcggg cggtgtgggc caggagtcga gcagcgtcag tgcgctgcgc agacctgcgg
     1561 ccgactttcg caggccgtgg tgcgcgagct cacggcgcct gcggccggcg gcgcgctgca
     1621 gccgggcctg gaactcgccc aggcggcgac gctggcccgc tcccttgctc cgcccggcga
     1681 gctcgcccag agtttgcagc agcacggcat cgtcgcgcgc cgcgctcagc gcccgcgcgg
     1741 cgtcgcgtaa cgcgcggttg ccgcggcccc aggccagctc actcaatagc ggccgcaaca
     1801 accgcaaggt ggcacgggcg gctttcatct gcttgcgtgc gcggtggatt tcttcatcgc
     1861 tcgctgcgct gcgcggcagc tccgcgactg cagtctcgag ctgccgctgc agtatgtcgc
     1921 gcataccgcg ggcaccgcag gtgccgtggc tcacctcgaa ccgatggctc atcgcgcttt
     1981 cccggtcgag tgcgcgccat gcgccgcgat cgcgccctcg agcgcccgtc tggcctcctc
     2041 cggtccgcca cggctactga tgctgaattc acgtccctgg aacgcgttgt acgacttgaa
     2101 gaagtgctcg atcgctctga gctcctcctc atcgagcgcg ctcagttcct gcagccgtgc
     2161 agggttgacc ggcgtatcga ccaccccgat gaggcgatcg ttgcggatga gcctggagcc
     2221 gtcgcgctgc tcggcgtgca gtacaccgat cagcctgacg gttaccagac atccgggaaa
     2281 gctcgccggc agttgcagca cgagtacatc gagcgcatcg ccatcctcgg cctcggttcc
     2341 cggaatggag ccgaagtcat aggggaatac cgcgcccggc ggcagtatgc ggctgacgcg
     2401 aaagctcgcg aattgctggt cgtacttgta cttgttgcgg ctgccggctg gcgtgtcgat
     2461 caccacgtgg acgcgcccgc tgccgctctc ccgcggagga atgtgctgag gcatcggtgc
     2521 acccgcgaaa actgctgccg cgctaatgag cgagagcgaa caacagcacc gtggcgctgc
     2581 cctcgtgggg cgtgtcagcg acaacgggcg caagcgtgcg caccgcgatg tactgatggc
     2641 cgtcggcgcc ccgatagctc acgggcgcat cgagcttgcg cccaccatcg acccggtgct
     2701 tccacagcac gcgcccggtg cgcgcatcga gcgccttgag ccagccgtcg ccggtgccgt
     2761 agaacaccag ccccccggca gtcacgagcg cgccggagga gatcggcagc gcctcgtgca
     2821 cactccagag cacgtgtcgt tcgatgggat cccaggcgcg cagctcgcgc cgtgcgaggt
     2881 ccgcgcgcgg ggagcacagc ggcg
//
LOCUS       DATDMP010000647         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_990234, whole genome shotgun sequence.
ACCESSION   DATDMP010000647 DATDMP010000000
VERSION     DATDMP010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990234"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1267)
                     /locus_tag="VI195_11370"
     CDS             complement(65..1267)
                     /locus_tag="VI195_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825034.1"
                     /translation="MPSSISSSEAALPGGLALRGTPSPQPHDHYARQTAADRPGIAQP
                     VPSRPVPVQPWGRIFLIAVALLALLIACWEGYWRNFGVKPGYRNTYGLWAIQRRRIDA
                     GEGNATVLLGDSRLYYDVALPVWERLDGHRPIQLAFEGTSPLTYLEDLAADPHFTGRV
                     LISVAPDLFFSGFEYRGGAVRYTRQESPSQRIGQRLSMRLIEPFLAFEDPDFSLATVL
                     ERQPWPERPGKHVFLEPRKLGYHEADRNTYLWDKVADDPQYRELWHRIWRDDFQPSPD
                     DPTPEEARKIEREQIARAARLVAKLRSRGVKVLFVRMPSAGEYLDYENSHFPRKRTWD
                     ALIAATAAPGIYFEDYPELQGYYLPEWSHMTRAEAERFTAALYRVIMRDFWGASAAPA
                     SSARTPAR"
     gene            complement(1242..>2160)
                     /locus_tag="VI195_11375"
     CDS             complement(1242..>2160)
                     /locus_tag="VI195_11375"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY6825035.1"
                     /translation="GPIMRPTELVPQFAPPRRASLDQLRFGLGLMTLGLFQKIVLADS
                     FLASVAERVYDSDKVPGALDAWAATLAFSGQIFCDFAGYSTTAIGAALCLGFAMPDNF
                     RFPYAAVGFSDFWRRWHITLSSWLRDYLYIPLGGNRHGVRRTYLALMGTMLLGGLWHG
                     ANWTFVAWGGLHGLYLALERALRARFSDYRPGPVAFVLLGLLTYTLVNITWVFFRAKT
                     FGKAWVVLRGMAGQNAGARPILATFSLLSVAAIVGALLVTHWLMRARTLESVVARAPA
                     VAVAAVWALMAFAIVIAQGSGSAFIYFQF"
BASE COUNT          395 a          718 c          738 g          309 t
ORIGIN      
        1 ggcttcaacc gcaagccacg cggctgggtg aagaatccct cctgaggacc ctgcccgccg
       61 ctggctagcg cgccggggtt cgagctgagg aggcgggcgc ggcggacgca ccccagaaat
      121 cgcgcatgat cacgcggtaa agtgccgcgg tgaatcgctc ggcctccgca cgcgtcatgt
      181 gtgaccactc gggaaggtag taaccctgca gctcgggata gtcctcgaag tagatgcccg
      241 gggcagcggt cgcagcgatc agcgcgtccc aggtccgttt acgcgggaaa tggctattct
      301 cgtaatccag gtactcgccg gcgctcggca tgcgcacgaa cagcaccttc acgccccgtg
      361 agcgcagctt cgcaaccagc cgcgcggcgc gcgcgatctg ctcccgttcg atcttgcggg
      421 cttcctccgg tgtcggatcg tcgggggagg gctggaaatc gtcccgccag attcggtgcc
      481 acagctcgcg atactgcgga tcgtcggcca ccttgtccca caggtaggtg ttgcggtctg
      541 cctcgtgata accgagcttg cgcggctcga ggaacacatg cttgcccggg cgctcgggcc
      601 agggctgccg ttcgagcacg gtggcgagtg agaagtccgg atcctcgaag gccaggaacg
      661 gttcgatcag gcgcatggac agccgctggc cgatgcgctg cgacggcgat tcctggcgcg
      721 tgtagcgcac cgctccgccg cggtattcga aacccgagaa gaacaggtcg ggtgccacgc
      781 tgatcagcac gcgcccggta aagtgcggat ccgcagcgag atcctccaga tacgtcaggg
      841 gcgatgtgcc ttcgaaggcc agttggatcg gtcggtgccc atcgagacgc tcccacaccg
      901 gcagcgcgac gtcgtagtag aggcgcgagt cgcccagcag cacggtcgca ttgccctcgc
      961 ccgcatcgat gcggcgccgc tggatggccc ataggccgta agtgttgcga tagccgggct
     1021 tgacaccgaa attgcgccag tagccttccc agcacgcgat cagcagtgcg agcagcgcca
     1081 cggcgatgag gaagatgcgg ccccacggct gtaccggcac cggacggctg gggacgggct
     1141 gcgcgatgcc cggccggtcc gccgcggtct gacgcgcata gtggtcgtgc ggctgcggag
     1201 agggcgtgcc gcgcagcgcg aggccgccgg gcagcgccgc ctcagaactg gaaatagatg
     1261 aaggcactgc cgctcccctg cgcaatcacg atcgcgaacg ccatcagcgc ccacaccgcg
     1321 gccactgcca cggccggcgc gcgtgccacc accgattcga gcgtgcgcgc gcgcatcagc
     1381 cagtgagtca ccagcagcgc gccgacgatc gccgccaccg acagcagact gaaggtcgcg
     1441 agtatcgggc gcgcgccggc gttctgacct gccatgccgc gcagcactac ccaggccttg
     1501 ccgaaagtct tggcgcggaa gaacacccag gtgatgttca ccagtgtgta ggtgagcagc
     1561 cccagcagca cgaacgccac cggtccggga cggtagtcgg aaaagcgcgc gcgcagtgca
     1621 cgctcgagcg ccaggtacag tccgtgcagt ccgccccagg ctacgaaggt ccagttcgcg
     1681 ccatgccaca gaccaccgag cagcatggtg cccatgagcg ccaggtaggt gcggcgcacg
     1741 ccgtgccggt taccaccgag tggaatgtac aggtagtcgc gcagccacga ggacagcgta
     1801 atgtgccagc gacgccagaa gtccgagaat ccgaccgccg cgtaggggaa acggaagttg
     1861 tccggcatgg cgaatcccag gcacagcgcc gcaccgattg cggtggtcga atagccggcg
     1921 aagtcgcaga agatctgccc gctgaaggcg agcgtcgccg cccaggcatc cagcgcgcca
     1981 ggaaccttgt cgctgtcgta gacgcgctcg gcgacgctgg cgaggaagct gtcggcgagc
     2041 acgatcttct ggaacagtcc cagcgtcatc agccccaggc cgaagcgcag ctggtccagg
     2101 ctcgcgcggc gcggcggcgc gaactgcggc accagctcgg tcgggcgcat gatcggaccg
//
LOCUS       DATDMP010000648         2330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1324700, whole genome shotgun sequence.
ACCESSION   DATDMP010000648 DATDMP010000000
VERSION     DATDMP010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324700"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1660)
                     /locus_tag="VI195_11380"
     CDS             complement(113..1660)
                     /locus_tag="VI195_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY6825036.1"
                     /translation="MASTAPAPALPTGTERLMITLAVMSATLIQVLDTTIVNVALPHM
                     AGELGATTEQISWVLTSYLVSSAIVMPLTGYFADTRGRKRFLLICICGFVAASALCSI
                     AQNLVQIVGFRLLQGVFGAALVPLSQAIMTDAYPPEDRGKAMAIWGLGVMVGPVLGPT
                     LGGWLTDVASWRWTFYINVPIGALSFFLASQYVPAGETRERHMDWTGLALLGSGVAGL
                     QYMLDRGTSQDWFAANDIRVSAALSAIGLAGFAWWSLRLGPRALFDLRIFGDRNFAMA
                     CGVMLAMGLGLFGGLVLQPILLEGLLGYPIVTTGLVMAPRGVATAIAMIVVGRLVGKH
                     DARVLVGVGMLLSAAGSWAMTGYSLEVTTFWIIWPAFLQGLGLGLIFVPLSTIAYATM
                     ERARIPEAAGIYSLVRTIGAAAGISVVTTLMTRQAQVIWNELGAHVTRFNPALIEYLR
                     PLHLSPDDPRAMALVAAQIGQQAEMGAILDVFKVTTWSFLLMLPLVFLLRRSKRPGAP
                     PPAAAME"
     gene            complement(1664..2098)
                     /locus_tag="VI195_11385"
     CDS             complement(1664..2098)
                     /locus_tag="VI195_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6825037.1"
                     /translation="MYHKVLLCYDGTAEGRRALRQGADVVLCMKSEAYLLAIVQSHIA
                     SAIPEAVTPALAESEDRRACSLLEEGVQWLRDRGLSAAGSLEYGNAVDCIAATAERIG
                     ADLIVLGHRNRSRLARWWSQSDEQSLLERARCSILVAAAVED"
     gene            complement(2102..>2330)
                     /locus_tag="VI195_11390"
     CDS             complement(2102..>2330)
                     /locus_tag="VI195_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY6825038.1"
                     /translation="AEIRSDMYPDRVFHGTVQSLAGGSGAAFSLLPPQNATGNWVKVT
                     QRVPVRIKVEDPDPQHPLRIGTSTTVKVRKE"
BASE COUNT          375 a          831 c          809 g          315 t
ORIGIN      
        1 gtgccccgcc gacccgcagc ggcgcgcctt cggcatcgtc gagcgcacgc tgaaccaggg
       61 tgaagacggg cggcgcgtgg tcaccgtcct gttctgatcc accgccgccg gttcactcca
      121 tcgccgcggc gggcggcggc gcccccggac gcttgctgcg ccgcaggagg aacaccagcg
      181 gcagcatcag caggaagctc caggtcgtca ccttgaacac gtcgaggatc gcgcccatct
      241 cggcctgctg cccgatctgc gcggcgacga gcgccatggc gcgcgggtcg tcgggcgaaa
      301 ggtgcagcgg ccgcaggtat tcgatgagcg ccggattgaa gcgcgtcacg tgcgcgccga
      361 gctcgttcca gatcacctgc gcctggcgcg tcatgagcgt cgtgaccacc gagatgcccg
      421 cggccgcgcc gatggtgcgc acgaggctgt agatgccggc ggcctccggg atgcgtgcac
      481 gctccatggt ggcgtacgcg atggtggaga gcggcacgaa gatgaggccc agtcccagcc
      541 cctgcaggaa cgcgggccag atgatccaga aggtcgtcac ctcgagcgag tagcccgtca
      601 tcgcccagga gccggcagcg ctgaggagca tgcccacgcc caccagcacg cgcgcatcgt
      661 gcttgccgac gaggcggccc acgacgatca tcgcgatggc ggtcgccacg cccctcggcg
      721 ccatcacgag ccccgtcgtg acgatgggat agcccagcag gccctccagc aggatcggct
      781 gcagcacgag tccgccgaag agcccgaggc ccatggcgag catcacgccg caggccatcg
      841 cgaagttgcg gtcgccgaag atgcgcaggt cgaagagcgc gcgcggcccg aggcgcaggc
      901 tccaccacgc gaagccggcc agcccgatcg cggagagcgc cgcgctcacg cggatgtcgt
      961 tcgcggcgaa ccagtcctgc gacgtgccgc ggtcgagcat gtactgcagt cccgccacgc
     1021 cgctgccgag gagtgccagc cccgtccagt ccatgtgccg ctcgcgcgtc tctcccgcgg
     1081 gcacgtattg cgaggcgagg aagaacgaga gcgcgccgat cggcacgttg atgtagaagg
     1141 tccatcgcca gctcgccacg tccgtgagcc agccgccgag cgtgggaccg agcaccggcc
     1201 ccaccatcac gcccaggccc cagatcgcca tcgccttgcc gcgatcctcg ggcggatacg
     1261 catcggtcat gatcgcctgc gacagcggca ccagcgccgc cccgaacacg ccctgcaaca
     1321 ggcggaagcc cacgatctgg acgaggttct gcgcgatgct gcacaacgcc gaggcggcca
     1381 cgaagccgca gatgcagatg agcaggaagc gcttgcgccc gcgcgtgtcc gcgaagtacc
     1441 cggtgagggg catcacgatc gcgctggaga cgagatagct ggtgagcacc cagctgatct
     1501 gctcggtcgt ggcacccagc tcgcccgcca tgtgcggcag cgccacgttg acgatggtcg
     1561 tgtcgagcac ctggatcagc gtcgccgaca tcacggccag cgtgatcatc aggcgctcgg
     1621 tgccggtggg cagggcgggc gccggggccg tggacgccat gcgctagtcc tcgaccgcgg
     1681 ccgcgacgag gatgctgcag cgcgcgcgct ccagcagcga ttgctcgtcc gactggctcc
     1741 accagcgcgc gagccggctg cgattgcgat gcccgagcac gatcaggtcc gcgccgatcc
     1801 gctcggccgt cgccgcgatg cagtcgaccg cgttgccgta ctcgagcgat ccggccgcgg
     1861 agagcccgcg atcgcgcagc cattgcacgc cctcctcgag gagcgagcag gcgcgccggt
     1921 cctcgctctc ggcgagcgcg ggcgtgacgg cctcgggaat cgcggaagcg atgtggctct
     1981 gcacgatcgc gagcaggtac gcctccgact tcatgcacag cacgacgtcg gctccctggc
     2041 gcagggcgcg gcgcccctcg gccgtgccgt cgtagcaaag cagcaccttg tggtacacgc
     2101 cctactcctt gcgcactttc accgtggtgc tggtgccgat gcgcagcgga tgctggggat
     2161 ccggatcctc caccttgatg cgcacgggca cgcgctgcgt gaccttcacc cagttgccgg
     2221 tggcgttctg cggcggcagc agcgagaacg cggcgccgct gccgccggcg aggctctgca
     2281 ccgtgccgtg gaagacgcgg tcgggataca tgtcggagcg gatctcggcc
//
LOCUS       DATDMP010000649         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1103652, whole genome shotgun sequence.
ACCESSION   DATDMP010000649 DATDMP010000000
VERSION     DATDMP010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103652"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2176
                     /locus_tag="VI195_11395"
     CDS             <1..>2176
                     /locus_tag="VI195_11395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY6825039.1"
                     /translation="RRALQDTLTAVRLKTPIAECGFALFPNGERHLRSRARLAGTYPP
                     ELLAETLRVAERCRFSLDELRYEYPRELVPPGETPASHLRSLTYEGARRRWPGGVPLS
                     ERAAIEHELALIAELDYEPYFLTVHDIVAYARSRGILCQGRGSAANSRVCYCLGITSV
                     DPQRGAALLFERFISRERNEPPDIDIDFEHEQREEVLQYVYGKYGRERAALAATVIMY
                     RPRSALRDLGKAFGLTPAESARLSRVMQWWDGSTAMGERLRAAGFDTGSPWLARLLPL
                     AGELVAHPGFPRHLSQHVGGFVISQGLLEELVPIENAAMPQRTVVQWDKDDLNDLGLL
                     KVDLLALGMLTALKRAFTLVNDYRGTHHALGELPAEDPGVYDMICRADTIGVFQIESR
                     AQMAMLPRLQPRSYYDLVIEIAIVRPGPIQGDMVHPYLRRRSGREPVEYPGEEVRAVL
                     ERTLGVPIFQEQVMQIAIVAAGFTPGEADALRRAMGAWKRTGGLDPFRERLLEGMRAR
                     GYPAQFAQRIYQQMLGFGEYGFPQSHAMSFALLVYDSAWLKCYEPAAFTCALLNSQPM
                     GFYAPAQLVRDARAHGVEVRAVDACVSSWDSTLERRADGEPALRLGLRLVKSLSRTGA
                     ERLIGARRALPFAGVADLAARAALDRGDLEALAAAGAFASLSGNRHLAFWEVAGTERP
                     LPLAPAPRQGVAATEGRPLLMAPSEGEEICADYASLGLTLGRH"
BASE COUNT          291 a          776 c          785 g          324 t
ORIGIN      
        1 gcgccgcgcg ctccaggaca cgctgaccgc ggtgcggctg aaaaccccca tcgccgaatg
       61 cggattcgcc ttgtttccca acggcgagcg gcatctgcgc tcgcgcgcgc gcctcgccgg
      121 cacgtatccg ccggagctcc tggccgagac gctgcgcgtg gccgagcgct gccgcttctc
      181 gctcgacgag ctgcgctacg agtacccgcg cgagctggtg ccgcccgggg agaccccggc
      241 gagtcatctc cgaagtctca cctacgaggg cgcgcgccgg cgctggcccg gcggcgtgcc
      301 gctgagcgag cgcgccgcca tcgagcacga gctcgcgctc atcgccgagc tcgactacga
      361 gccttacttc ctcaccgttc acgacatcgt cgcctacgca cgctcccgcg ggatcctgtg
      421 ccagggacgg ggctcggcgg ccaactcccg cgtgtgctac tgcttaggca tcacctcggt
      481 ggacccgcag cggggcgcgg cgctgctgtt cgagcgcttc atctcgcgcg agcgcaacga
      541 gccgcccgac atcgacatcg acttcgagca cgagcagcgc gaggaggtgc tgcagtacgt
      601 ctacggcaag tacgggcgcg agcgtgcggc actcgccgcg acggtcatca tgtaccggcc
      661 gcgcagcgcg ctgcgcgatc tcggcaaggc cttcggcctg acgccggcgg agagcgcgcg
      721 cctctcccgg gtcatgcagt ggtgggacgg cagcacggcg atgggcgagc ggttgcgcgc
      781 ggcgggcttc gataccggga gtccctggct cgcgcgcctg ctgccgctcg ccggcgagct
      841 ggtggctcac ccgggcttcc cccgccacct gtcgcagcac gtcggcggct tcgtgatctc
      901 gcagggcctg ctcgaggagc tggtgcccat cgagaacgcc gccatgccgc agcgcaccgt
      961 ggtgcagtgg gacaaggacg atctcaacga cctggggctg ctgaaagtcg acctgctggc
     1021 gctcgggatg ctgacggcgc tcaagcgcgc cttcacgctg gtcaacgact accgcggcac
     1081 gcaccacgcg ctcggcgagc tgccggccga ggatccgggc gtctacgaca tgatctgccg
     1141 cgccgacacc atcggagtgt tccagatcga atcgcgcgcg cagatggcga tgctgccgcg
     1201 cctgcagccg cgcagttact acgatctcgt catcgagatc gccatcgtgc gccccggccc
     1261 catccagggg gacatggtgc atccctacct gcggcgccgc tcggggaggg agccggtcga
     1321 gtatccgggg gaggaggtgc gcgcggtact cgagcgcacg ctcggcgtgc cgatcttcca
     1381 ggaacaggtg atgcagatcg cgatcgtcgc cgcgggcttc acccccgggg aagcggacgc
     1441 gctgcgtcgc gcgatgggcg cgtggaagag gaccgggggc ctcgatccgt tccgcgagcg
     1501 gctgctcgaa ggcatgcgcg cgcgcggcta tccggcgcag ttcgcccagc gcatctacca
     1561 gcagatgctc ggcttcggcg agtacggctt cccccagtcg cacgcgatga gcttcgcgct
     1621 gctggtgtac gactcggcgt ggctgaagtg ctacgagccg gcggccttca cctgtgcgct
     1681 gctcaacagt cagccgatgg gcttctatgc gcccgcccag ctggtgcgcg atgcgcgtgc
     1741 gcacggcgtc gaggtacgcg ccgtggatgc ctgcgtctca tcgtgggact cgacgctcga
     1801 gcgccgcgcg gacggcgagc cggcactgcg cctggggctg cggctggtga agtcgctgtc
     1861 gcgcaccggg gcggagcgcc tgatcggcgc gcgccgcgcg ctgcccttcg cgggggtcgc
     1921 ggatctcgcc gcacgcgcgg cgctggaccg cggcgatctc gaggcgctgg ccgcggccgg
     1981 ggcgttcgcc tctctgagcg gcaaccggca tctggcgttc tgggaagtgg cgggcacgga
     2041 gcggccgctg ccgctcgctc ccgccccgcg gcagggcgtc gccgccacgg aaggccggcc
     2101 gctgctcatg gcgccgtcgg aaggcgagga gatctgcgcc gactacgcct cgctcgggct
     2161 caccctgggg aggcat
//
LOCUS       DATDMP010000650         3489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1657656, whole genome shotgun sequence.
ACCESSION   DATDMP010000650 DATDMP010000000
VERSION     DATDMP010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657656"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VI195_11400"
     CDS             <1..961
                     /locus_tag="VI195_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY6825040.1"
                     /translation="DALAALAERACALEWTRPELTDEPRLTILGGRHPIVERFSDAPF
                     VPNDLRLDAARRMLIITGPNMGGKSTYMRQAALIAVLAHLGSYVPAERAVLGPLDRVF
                     TRIGAADDLAGGRSTFMVEMTEAANILHNASAHSLILLDEIGRGTSTFDGLSLAWAMA
                     RDIAARLRAFTLFATHYFELTTLVTEVEGCANVHLDATEHGDGIVFLHAVKEGPANRS
                     YGLQVAQLAGVPRGVIAEARRYLERLESERDRQRAVGREAGGPQAELPLFAPAACDSL
                     SSGSPQAAAPDALREALAAVDPDALSPRQALEALYALRRLLDS"
     gene            complement(986..1615)
                     /locus_tag="VI195_11405"
     CDS             complement(986..1615)
                     /locus_tag="VI195_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6825041.1"
                     /translation="MAAVVYVHGLWMPGEESLLLKTRLSHEFALALHAFRYSAVTSSM
                     SHITERLAEFVHALASPEVHFIGHSLGGLVIYRFLERFPEQPPGRVVFLGTPSVASRA
                     AEHAARFAPIAHLMGHSVADELLQPRERRWTQARPLGIVAGTQPIGLGQLLAHFQEAN
                     DGTVAVSETRMPGATDHISLPVSHLGMLISARVAHETGRFLNYGHFSLR"
     gene            complement(1632..3416)
                     /gene="aspS"
                     /locus_tag="VI195_11410"
     CDS             complement(1632..3416)
                     /gene="aspS"
                     /locus_tag="VI195_11410"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY6825042.1"
                     /translation="MAPIAARSRYYCGQVNEQLIGQVITVAGWVHRRRDHGGVIFVDL
                     RDREGLLQIVFDPTAAAVFREAERLRNEFVVRVRGRLRPRPSGTVNPNLASGGVELLA
                     EELELLNRSEPLPFQLDEQVNEEVRLRYRYLDLRREVMSQRLRLRHRLTRSMRAYLDT
                     HGFVDLETPMLTKATPEGARDYLVPSRTHPGKFFALPQSPQIFKQLLMIAGFDRYYQI
                     ARCFRDEDLRADRQPEFTQLDVETSFLSQDEIMKLMEGLVRHFFHEVLGAQLPDPFPR
                     MSHAEALRRFGSDKPDLRNPLELTDVADLVRDCEFKVFAGPAKDPQGRVAALRVPGGG
                     GMPRSQIDECTAFVTRYGAKGLAYIKVNARAGGREGLQSPIIKFLSDAALSGILERTQ
                     ATDNDVIFFGADSAKVVGDALGALRLKLGQDLALLAGGWRPLWVIEFPMFEWDEQERR
                     WVAMHHPFTAPLNDDPAALRAAPAAALAKAYDMVLNGSEIGGGSVRIHREEMQSTVFS
                     LLGIDAAEAQQKFGFLLDALKFGAPPHGGIAFGLDRLAMLMAGADSIREVIAFPKTQT
                     AADPLTDAPTEVSEAQLRELHVRVRRPQ"
BASE COUNT          574 a         1209 c         1143 g          563 t
ORIGIN      
        1 cgacgcgctc gccgcgctcg ccgagcgcgc ctgcgcgctc gagtggacgc ggcccgagct
       61 taccgatgag ccgcgcctca cgatcctcgg cggccgacac ccgatcgtcg agcgcttcag
      121 cgatgcgccg ttcgtgccga acgatctgcg tctcgatgcc gcgcggcgca tgctcatcat
      181 caccggaccc aacatgggtg gtaagtccac ctatatgcgt caggccgcgc tcatcgccgt
      241 gctagcccac ctcggcagct acgtgcccgc cgagcgcgcc gtgctggggc cgctcgatcg
      301 cgtgttcacc cgcatcggtg cggctgacga tctggcgggc ggccgctcga ccttcatggt
      361 cgagatgacc gaagccgcca acatattgca caacgccagc gcacacagtc tgatcctgct
      421 cgatgagatc ggccgcggca ccagcacgtt cgatggactg tcgctcgcct gggccatggc
      481 gcgcgacatc gccgcgcgcc tgcgcgcctt cacgcttttc gcgacgcact atttcgagct
      541 cacgactctc gtcaccgagg tcgaagggtg cgcgaacgtg catctggatg ccaccgagca
      601 cggcgatggc atcgtgttcc tgcacgcggt caaggaagga cccgccaacc gcagctacgg
      661 actgcaggtc gcgcagctcg ccggggtacc acgtggcgtg atcgccgagg cacgccgcta
      721 tctcgagcgg ctcgagagcg agcgcgatcg ccagcgcgcg gtcgggcgcg aggctggcgg
      781 cccccaggcg gagctgccgc tgttcgcgcc ggcggcttgc gactccttat cttccgggtc
      841 gccccaggcg gctgcgcccg atgcgctgcg cgaggcactc gccgcggtcg accccgatgc
      901 gctttcgccc cgccaggccc tcgaagcgct ctatgcgctg cgccgcctgc tcgattcctg
      961 agcgtcgcgg cgcaccgcgg ctggttcagc gcagcgagaa gtgcccgtag ttgaggaagc
     1021 gaccggtttc gtgcgccacg cgcgccgaga tgagcattcc aagatgactc accggcagtg
     1081 aaatatggtc ggtcgcgccc ggcatgcggg tttcactcac ggccacggtg ccatcgttgg
     1141 cctcctggaa gtgcgcgagc agctgtccca gcccgatcgg ttgcgtgccc gcgacgatgc
     1201 cgaggggccg cgcttgcgtc cagcgccgct cacgtggctg caacagctcg tccgcgaccg
     1261 agtgccccat gaggtgcgca atgggagcaa agcgcgccgc atgttcggcg gcacggctgg
     1321 caacgctcgg cgtccccaga aaaaccaccc ggccgggagg ctgctcggga aagcgctcga
     1381 gaaagcggta gatcaccagt ccgccgaggc tgtggccgat gaagtgcacc tccggtgatg
     1441 cgagagcatg cacgaactcc gcgagacgct cggtgatgtg gctcatgctg gaggtgacgg
     1501 ccgagtagcg gaaggcatgc agagcgaggg cgaactcgtg cgagagacgg gtcttcagca
     1561 gcagcgactc ctcgcccggc atccacagcc catgcacata gacgaccgcc gccacaaccg
     1621 agctcagcgc ctcactgcgg ccggcgcacc cgcacgtgca gctcgcgcag ctgcgcctca
     1681 ctcacctcgg tcggcgcatc ggtgagcgga tcggcagccg tctgggtctt cgggaaggcg
     1741 atgacctcgc ggatgctgtc ggctccggcc atgagcatcg ccagccggtc gagtccaaag
     1801 gcgatgcccc cgtgcggtgg cgccccgaac ttgagcgcat cgagcagaaa cccgaatttc
     1861 tgctgcgcct ccgcggcgtc gatgccgagc aggctgaaga ccgtggactg catttcctcg
     1921 cggtggatac gcaccgagcc gccaccgatc tccgagccgt tgagcaccat gtcgtaagcc
     1981 ttggcgagcg ctgccgccgg tgccgcgcgc agcgccgcgg gatcgtcgtt cagcggagcg
     2041 gtgaaggggt ggtgcatggc cacccagcgc cgctcctgct catcccactc gaacatcggg
     2101 aactcgatca cccacagcgg ccgccaaccc ccggcgagca gcgcgagatc ctgcccgagc
     2161 ttcagacgca gcgcaccgag cgcatcgccc accactttgg cgctgtcggc gccgaagaaa
     2221 atgacgtcgt tgtcggttgc ctgggtgcgc tcgagaatgc cgctgagcgc agcatcactc
     2281 aggaatttga tgatcggcga ttgcaggcct tcgcgcccgc ctgcacgagc attcaccttg
     2341 atgtaggcga gccccttggc gccatagcgc gtcacgaaag ccgtgcactc atcgatctgc
     2401 gagcgcggca tgccgccgcc gcccggcacg cgcagcgccg ctacccttcc ctgggggtcc
     2461 ttcgcgggac cggcaaacac cttgaactcg cagtcgcgca cgaggtccgc gacgtcggtg
     2521 agctcgagtg ggttgcgcag atctggcttg tccgagccga agcggcgcag cgcttctgcg
     2581 tggctcatgc gcgggaaggg gtcggggagc tgtgcaccga gcacttcgtg gaagaagtgc
     2641 cgcaccagcc cctccatcag cttcatgatc tcgtcctgcg agaggaacga ggtctcgacg
     2701 tcgagctggg tgaactccgg ctgccgatcc gcgcgcagat cctcgtcgcg gaagcagcgc
     2761 gcgatctgat agtagcggtc gaagcccgcg atcatgagca gctgcttgaa aatctgcggc
     2821 gattgcggca gcgcgaagaa tttgcccggg tgcgtgcggc tcggcaccag gtagtcgcgt
     2881 gcgccttccg gcgtcgcctt ggtgagcatc ggcgtctcga gatcgacgaa gccgtgggtg
     2941 tcgaggtacg cgcgcatcga gcgcgtcagc cgatgccgca gacgcagccg ctggctcatc
     3001 acctcgcgac gcagatcgag atagcgataa cgcaggcgca cttcttcgtt cacctgctca
     3061 tcgagctgga atggcagcgg ctcggagcgg ttgagcagct cgagctcctc ggccagcagc
     3121 tcgacgcccc cggaggcgag attcgggttc accgtgccgc tgggccgcgg gcgcaggcgt
     3181 ccgcgtacgc gcactacgaa ctcgttgcgt agccgctcgg cctcgcggaa caccgcagca
     3241 gccgtcggat cgaacacgat ctgcagcagt ccctcacgat cgcgcaggtc gacgaatatc
     3301 actccgccgt gatcgcgccg gcggtgcacc caaccggcga ccgttatcac ctggccgatg
     3361 agctgctcgt tgacctgtcc gcagtaatat cttgaccgcg ctgctatcgg cgccatgcct
     3421 gttcccaaca gtcatcagtc cgatgcccgc ttcgcgcttc ctctgcgagc acctgctcca
     3481 gaagctcag
//
LOCUS       DATDMP010000651         3036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1991776, whole genome shotgun sequence.
ACCESSION   DATDMP010000651 DATDMP010000000
VERSION     DATDMP010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991776"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VI195_11415"
     CDS             <1..988
                     /locus_tag="VI195_11415"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY6825043.1"
                     /translation="VQATFEQIDLVKTMCARYSHDLAMAYSAADIRRLHRAGLIASLI
                     GVEGGHQINDSLVVLRAYYEVGARYLTLTHSSNTAWADSATDTPVHHGLTPFGKEVVR
                     EMNRLGMLVDLAHVSEETMRAALSVSAAPVIFSHSSARALVDHPRDVSDEVLRLVARN
                     GGIVMVNFAPPYVSDARRRWAAERGAEEARNNSPPFGGLYIGQPARAAAALKSWDAAH
                     PKPPVTLAEVADHIEHIRQVAGADYVGLGSDFDGIPETPTGLEGVDRFPALLQELARR
                     GWSDADLAKVAGANLLRVMAQAESVSARLRTERSASTATLEALDHPPTAGAQ"
     gene            complement(1656..>3036)
                     /locus_tag="VI195_11420"
     CDS             complement(1656..>3036)
                     /locus_tag="VI195_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6825044.1"
                     /translation="QSAATQLLRAKACLALARPAEALRSLDRARELAPQEFNAHFDRG
                     VALTRLRRNEEALASFDLALALDPKLHEAANNRGAVLVRMFRPAEALADFARAVALKP
                     DYIEAHTNAGIALRGLARSAEALASLDRALAFSPGDPSARWCKALLKLSAGDFREGWP
                     LYEARLEREPLRGQLRNFDQPRWRGEASIAGKQLFVYAEQGLGDTLQFCRYIPRLEQM
                     GAQVILEVQAVLKPLLGSLPMRGTLIGRGERVPDFDLHIPLGSLPLALGTDLGTIPRE
                     VPYLHVEPEPAREWAQRLASLGGYKVGLNWQGNLQSEKLAALEARSFPLSTAAPLARI
                     EGITLVSLQKGAGSEERAMVDFGNAIAQLTDPQHMGPEELARDTAAILKGLDLVITAD
                     TALAHLAGALGVRVWVVLQAVPDWRWLTERSDSPWYPTMRLFRQRAPGDWSELFGRVA
                     AELATLNPH"
BASE COUNT          511 a         1011 c         1022 g          492 t
ORIGIN      
        1 ggtgcaggcg acgttcgagc agatcgacct ggtcaagacg atgtgtgcgc gctacagcca
       61 cgatctggca atggcctaca gcgccgcgga catacgccgg ctgcaccgcg ccggactcat
      121 cgcctcgctg atcggagtgg aaggcggcca tcagatcaac gattcactcg tcgtgctgcg
      181 cgcttactac gaggtcggcg cccgctatct gactcttacg cattcatcca acaccgcctg
      241 ggccgactcg gccacggaca cgccggtgca ccatggcctg acgcctttcg gcaaggaggt
      301 ggtacgggag atgaaccgcc tgggtatgct ggtcgacctc gcgcatgtct cggaagagac
      361 catgcgcgca gcgctcagcg tgagcgcggc gccggtcatc ttttcacact cctcggcgcg
      421 cgcgctggtg gatcatccgc gcgatgtctc cgatgaggtg ctgcgcctgg ttgcgcggaa
      481 tggcggcatc gtgatggtca acttcgcgcc tccgtacgtg tccgacgcgc gccgccgctg
      541 ggccgcggag cgtggcgctg aagaggcgcg caacaacagc ccgcccttcg gcgggcttta
      601 catcggccag cccgcgcgcg cggcggctgc actcaagtcc tgggacgccg cgcacccgaa
      661 gccgccggtc acactggccg aggtggctga ccatatcgag cacatccgcc aggtggccgg
      721 cgccgactat gtggggctcg gatcggactt cgacggcatt cccgagaccc cgaccggcct
      781 cgagggcgtc gatcgattcc cggctctgct gcaggagctc gcacggcgcg gctggagcga
      841 tgcggatctc gccaaggttg ccggtgcaaa cctgctgcgc gtcatggcgc aggcggagag
      901 cgtgagcgca cggctgcgca cggagcgttc cgcctccacg gcgaccctgg aagcactgga
      961 tcacccgccg acggccgggg cacagtgatc agcccagcat cggtctctca gaagtgcgcg
     1021 gtaaacgcga gcggtcatcc accgccgggg cagccgcccg gcccgatctg cgcgcagccg
     1081 ggcgtcggtc gcgctaactg gtcccgggta gcatgaactg cagcgtcacg ggcttctcgc
     1141 tgagcggccg tctcacacac tctgacgcca acacggctgc cggcggggac ttccatatga
     1201 cacgttacct tcgagtcggt cttcgcagcc ttcgactcgt cagaagttgc atcggtgggt
     1261 gcgatggcag cgttggagat cgcggagtcc gtcccgcccg caacggttgc gccttgcgca
     1321 gaagcggcga ctgccgcggg tgccttggtg gccgctgtgt gctcgggagg tgacgaagcg
     1381 cagccagcaa tgacgctcgc cagaacagtg acgaaaacct ggcctgagcc ccggatgggc
     1441 gccacgcgtt cgtgggaaag cataagctct caaagtggtc gcacgatgtg ccgcggcaca
     1501 ctcggcgccg ctgcattaaa gcgcctttgc aggctactct gtcaacacag cgatggtgct
     1561 cgggcgtaca accagcgcga tgtcaggagc attggccaaa ccgacgccgc gccggcgttg
     1621 cgcatgctcg actcatcggt gaccctgccc ggccgctagt gcgggttcaa cgtcgccagc
     1681 tcggcagcga cgcgcccgaa gagctccgac cagtcccccg gtgcgcgctg acggaacagt
     1741 cgcatcgtcg ggtaccaggg actgtcgcta cgctcggtga gccagcgcca gtccggcacc
     1801 gcctggagaa cgacccacac acgcacgccg agggcgccgg ccagatgggc gagcgcagtg
     1861 tcggcggtga tcaccaggtc gagtcccttc aatatcgcag cggtgtcgcg cgcgagctct
     1921 tctgggccca tgtgctgcgg atcggtgagt tgcgcgatgg cgttgccgaa atcgaccatg
     1981 gcgcgttcct cggagccggc acctttctgc agtgatacga gggttatccc ctcgatccgc
     2041 gccagcggcg cggccgtcga gagcgggaag gagcgtgcct cgagtgcagc aagcttctcg
     2101 gactggaggt tgccctgcca attcagcccg accttgtagc cccccaacga tgcgaggcgt
     2161 tgtgcccact ctcgggcggg ctccggctcg acgtgcaggt aagggacctc gcgcggaatc
     2221 gtcccgagat cagtgccgag cgccagcggc aggctcccca gcgggatgtg cagatcgaag
     2281 tccggcaccc gctcaccgcg gccaatgagc gtgccgcgca tcgggagcga gccgagcagc
     2341 ggcttgagca ctgcctgcac ctcgaggata acctgcgcgc ccatttgctc gagccgcggg
     2401 atgtagcgac agaattgcag cgtgtcgccg agtccctgtt ccgcatagac gaaaagttgc
     2461 ttacccgcga tcgaggcctc gcctctccag cgcggctggt cgaagttcct caattggccc
     2521 cgcaagggct cacgctcgag gcgggcctcg tagagtggcc agccctcgcg gaagtcgcca
     2581 gcgctgagct taagcagcgc cttgcaccag cgcgcacttg gatcgcccgg gctaaaggcg
     2641 agcgcacggt cgaggcttgc gagtgcttcc gcagaacgcg cgaggccacg cagagcgatg
     2701 ccagcattgg tatgggcctc gatgtaatcg ggcttcaggg cgacggcacg cgcgaaatcc
     2761 gcaagggcct ctgcggggcg gaacatgcgt accaggaccg cgccccggtt attagccgcc
     2821 tcatgtaatt ttgggtcgag agcgagcgcg agatcgaaac tcgcaagcgc ctcctcattg
     2881 cgccggagcc gcgtcagtgc gactccgcga tcgaaatgcg cgttgaactc ctgcggcgcg
     2941 agctcacgcg cgcggtcgag gcttctgagt gcttccgcgg gccgtgcgag cgcgaggcac
     3001 gctttcgcgc gcaacagctg cgttgcagcc gactgc
//
LOCUS       DATDMP010000652         3600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1546433, whole genome shotgun sequence.
ACCESSION   DATDMP010000652 DATDMP010000000
VERSION     DATDMP010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546433"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VI195_11425"
     CDS             <1..764
                     /locus_tag="VI195_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEY6825045.1"
                     /translation="NERPGRGLHGYARSFEFQHGRTLFAIGGQRETAFISMSGDGCAL
                     VQDWPALVALLRDRLDGRITRWDGATDDYEGRRSVDSAVEAYRAGGFNGRGRKPSCSV
                     DGDWIDPQGKGRTFYVGNRENGKLLRVYEKGKQLGEAQSPWVRWEVELHNVDRVIPWE
                     VLLEPGRYLAGAYPALTWVQEEASRIATLKKADGITYDRAVHYAKQSIGRLVNVMVER
                     EGSPEGVVEELRRDGVPKRLELTERLGLRGEKKKK"
     gene            761..997
                     /locus_tag="VI195_11430"
     CDS             761..997
                     /locus_tag="VI195_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825046.1"
                     /translation="MTFEADDFDWALGELLALVWRGKLAGRPAHLLEPRLFHDCMYVI
                     NAQPFEARKWAHSVIMGMEPFKATPRRYEVACAS"
     gene            1184..2227
                     /locus_tag="VI195_11435"
     CDS             1184..2227
                     /locus_tag="VI195_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY6825047.1"
                     /translation="MSLYKRGESWYVDITTPRGRRIRESAKTGDRKAAKEYHDRLKAE
                     AWRVDKLRERPSRTWDEAALRFLKEAEGKAIFRDYKRHIAFWTKHFSERPLEEISRHE
                     AADVVEVQAKTPATRNRYIACLRAVLMKACGAWEWIERAPKFKMYSEPKQRIRWISKE
                     EAETLLAAMPEWLADLARFALATGLRQANVLALEWTQVDLKRGVAWVHPDQAKARRAI
                     GVPLNEDAVAVIRRQLGKDLRFVFVGHDGTPLDHWSMPARKGWDAACKQAGITDFRWH
                     DLRHTWASWHVQQGTPLYVLKELGGWETLEMVKRYAHLAPEHLKEYAERVNFGATSQI
                     RHKEGVTKAEAVA"
     gene            complement(2232..2318)
                     /locus_tag="VI195_11440"
     tRNA            complement(2232..2318)
                     /locus_tag="VI195_11440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2282..2284),aa:Leu,seq:caa)
     gene            2357..3349
                     /gene="queA"
                     /locus_tag="VI195_11445"
     CDS             2357..3349
                     /gene="queA"
                     /locus_tag="VI195_11445"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY6825048.1"
                     /translation="MLVSEFDYELPSELIAQRPAAQRASSRLLHLDARSGALRDLRFS
                     DLPGLVDARDALVLNDTRVIKARLAARKASGGKAEIFIERALDARRALAMMRAGHSPK
                     AGAEFLVGNDVRVTVEGRAEDLYKVAFSEDIDRVLDRFGAVPLPPYISHPPAAEDAER
                     YQTVYASVPGAVAAPTAGLHFTPELLEEVKGRGAAVARVTLHVGVGTFQPVRTERIEE
                     HRMHSERYSIPAQTIQAIQGRRVLAVGTTSLRALETWQLTGRAQGESELFVAPGFRFQ
                     VVERLLTNFHLPKSTLLMLVSAFAGMENIRKAYAHAIAERYRFFSYGDAMLIDR"
     gene            3346..>3600
                     /locus_tag="VI195_11450"
     CDS             3346..>3600
                     /locus_tag="VI195_11450"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY6825049.1"
                     /translation="MNFELITSDGLARRGVLTLAHGRVDTPAFMPVGTYGSVKAMSPQ
                     ELRALGAQIVLGNTFHLWLRPGLEVIAAHGGLHRFMGWPRP"
BASE COUNT          658 a         1101 c         1236 g          605 t
ORIGIN      
        1 tcaacgagcg gccagggcgt ggcctgcacg gctacgctag gtcattcgag ttccagcacg
       61 gccgcacgct cttcgcgatc ggcggccagc gggaaacagc attcatctca atgtccgggg
      121 acggctgcgc gctggtccag gattggccag cactcgtcgc cctactccgg gatcggctgg
      181 acggtcggat cacgcgctgg gatggggcta cggacgacta cgaggggcgt agaagcgtcg
      241 attcggcggt agaggcctat cgggccgggg gctttaacgg acgcggcagg aagccgtcct
      301 gcagcgtcga tggcgactgg atcgacccgc agggcaaagg ccggacgttc tacgtaggga
      361 accgcgagaa cggcaagctc ctacgggtct acgagaaggg caagcagctg ggcgaggccc
      421 agtcaccttg ggttcgctgg gaggtcgagc ttcacaacgt agaccgcgtc atcccctggg
      481 aagtgctcct cgagccggga cgatacctcg cgggcgccta ccctgccctc acttgggtcc
      541 aggaggaggc aagccggatc gcgacgctga agaaggctga tggcatcacg tatgaccgcg
      601 ccgtccatta cgcgaagcag tccatcggac ggcttgtcaa cgtgatggtc gagcgcgagg
      661 gaagccccga aggagtggtg gaggagctgc gccgtgacgg cgtaccaaag cgcctggagc
      721 tcacggagcg gctcgggctc cgaggcgaga agaagaaaaa atgaccttcg aggccgatga
      781 tttcgattgg gcgcttggcg aactcctcgc gctcgtatgg cgcgggaaac tcgcgggccg
      841 ccccgcgcat ttgctcgagc cccggttatt tcacgattgt atgtacgtga tcaacgcgca
      901 gccattcgag gcgcgcaagt gggcgcacag cgtaatcatg ggcatggagc cgttcaaggc
      961 aacgccgagg cgttatgaag tcgcttgcgc ttcatgaagc ggctgcgttt cttcggatgc
     1021 atccggagga gcttcgccgg cgcgcgcgcc tcggactggt tccagcggcg aagccaggaa
     1081 agcgctgggt gtttctcgaa gaggatcttg ccgcctatct ccgcgcgctt tatgctgctc
     1141 ctcggcaagc gttgcgagcg acactcggaa aggaggcaag caattgtcgc tctacaaacg
     1201 cggtgagtcc tggtacgtgg acatcaccac gccgcgcggc cgccgaatta gagaaagcgc
     1261 taagaccggc gaccgaaaag cggcgaagga gtatcacgac cggctgaagg ccgaagcatg
     1321 gcgagtcgat aagctgcgcg agaggccttc gcggacgtgg gatgaagcag ccctgcggtt
     1381 tctgaaggaa gccgaaggca aggcgatttt tagggactac aagcggcaca ttgctttctg
     1441 gacgaagcat ttcagcgagc ggcccttgga agagatttcg cggcacgaag cggcggacgt
     1501 ggtcgaagtg caggccaaaa cgccggcaac gcgaaatcgc tacatcgctt gtcttcgggc
     1561 ggtcctcatg aaagcgtgcg gcgcctggga gtggatcgag cgggccccga agttcaagat
     1621 gtattcggag ccgaagcagc gcatccggtg gatcagcaag gaggaagcgg aaacgctcct
     1681 cgctgcgatg ccggagtggc tggcagacct cgcccgattt gccttggcga cagggctgcg
     1741 gcaggcaaac gtactggctc ttgagtggac gcaggtggat ctgaagcgcg gcgttgcctg
     1801 ggtgcatccg gatcaggcga aggcgcgacg ggcaatcggt gtccctctca acgaggatgc
     1861 ggtggcggtg attcgccggc agcttggcaa ggatctgcgc ttcgtcttcg ttggccacga
     1921 tggaacgccg ttggatcact ggtcgatgcc cgcccgcaag ggctgggatg cggcctgtaa
     1981 gcaggcgggc attacggact tccgctggca cgatctgcgg catacctggg cgagctggca
     2041 cgtgcagcaa ggaacgcccc tttacgtgct gaaggagctt ggaggctggg agactttgga
     2101 gatggtgaag cggtacgccc acctcgcccc cgagcacctg aaggagtacg cggagcgggt
     2161 caacttcggc gctacgtcac aaatacggca caaggaaggg gtgacgaagg ccgaagcagt
     2221 agcgtaagtg ctggtgccgg aagagagaat cgaactctca tgaccttgcg atcggcggat
     2281 tttgagtccg ctgcgtctac cagttccgcc attccggcca agggtgctga ggcggccgaa
     2341 attataattg gccgcgatgc tcgtctccga gttcgattac gagctgccga gcgagctcat
     2401 tgcgcagcgc cccgcggcgc aacgcgcctc cagccgtctc ctgcacctcg atgcccgcag
     2461 cggcgcgctg cgcgacctgc gcttttccga tcttcccggt ctcgtggacg cccgcgacgc
     2521 gctggtgctg aacgacacgc gcgtcatcaa ggcgcgcctg gcggcgcgca aggcgagcgg
     2581 cggcaaggcc gagatcttca tcgagcgcgc cctcgatgcg cgccgcgcgc tcgccatgat
     2641 gcgcgcgggc cattcgccca aggcgggcgc ggagttcctg gtcggcaatg acgtgcgcgt
     2701 gacggtggag gggcgcgccg aggacctcta caaagtcgcg ttttccgaag atatcgatcg
     2761 ggtcctcgat cgcttcggcg ccgtgccgct tccgccctat atctcgcatc cgccggccgc
     2821 cgaggacgcc gagcgctacc agaccgtcta cgcttcggtc ccgggcgcgg tcgcggcgcc
     2881 gacggccgga ttgcatttca ccccggagct gctcgaagag gtgaaaggac ggggcgcggc
     2941 cgtggcgcgc gttaccctcc acgtcggcgt cggcaccttc cagccggtac gcacggagag
     3001 aatcgaagag caccgcatgc acagcgagcg ctacagcatt cccgcgcaaa ccatccaggc
     3061 gatccagggc cggcgcgtgc tcgcggtggg caccacctcg ctgcgcgcgc tcgagacgtg
     3121 gcagctcacc ggccgcgcgc agggcgaaag cgagctcttc gtcgcgccgg ggttccgctt
     3181 ccaggtggtg gagcgcctcc ttaccaattt ccacctgccg aaatccacgc tcctcatgct
     3241 cgtcagcgcg ttcgccggga tggagaacat tcgcaaggcc tacgcgcacg ccatcgccga
     3301 gcgctatcgc ttcttttcgt acggcgatgc catgctcatc gaccggtgaa tttcgagctc
     3361 ataacaagcg atggattggc gcggcggggc gtcctcacgc tcgcgcacgg ccgggtggac
     3421 accccggcat tcatgccggt gggcacctac ggctcggtga aggcgatgtc gccgcaggag
     3481 ctgcgcgcgc ttggcgccca gatcgtgctc ggcaacacct tccacctgtg gctgcgtccc
     3541 ggcctcgagg tcatcgccgc gcacggcggc ctgcaccggt tcatggggtg gccgcggccg
//
LOCUS       DATDMP010000653         3291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1546450, whole genome shotgun sequence.
ACCESSION   DATDMP010000653 DATDMP010000000
VERSION     DATDMP010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546450"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VI195_11455"
     CDS             <1..1009
                     /locus_tag="VI195_11455"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825050.1"
                     /translation="GRVMEVLSEHTLMGWLEGYLLTGRHGFFATYEAFAHVVDSMFNQ
                     HAKWLDISKNHVRWRAAVASQNILLTSTVWRQDHNGFSHQDPGFIDLATNKSPSVTRV
                     YLPPDANTLLVVADRCLRSTDCINVIVADKQKHLQFTTIEQAIVHCAKGVGIIRAVSN
                     DEGSEPDVVMACCGDVATQEALAATSILRERLPELKVRFVNVVDLFKMQPASEHPHGS
                     SEREFDSLFTRDKPVIFNFHGYPWLIHKLCYRFKNSENIHVRGYKEKGNINTPLELAI
                     LNQVDRFDLVIDVIDRVPGLAERAAHLKEDMKNAIIDSLTYAHREGADRAEVANWVWP
                     G"
     gene            1006..2721
                     /gene="pgm"
                     /locus_tag="VI195_11460"
     CDS             1006..2721
                     /gene="pgm"
                     /locus_tag="VI195_11460"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEY6825051.1"
                     /translation="MNGKPEAGSGRHEDATWPSGVHPLAGKPAPASVLIDPDKLCREY
                     YERRPDSADPAQRVSFGTSGHRGSALHGSFNEFHVLAITQAICEYRSKESITGPLFIA
                     RDTHALSGPALRTALEVLAANDVQTMIDRSDGYTPTPVLSHAILTHNRARPARQADGI
                     VITPSHNPPEDGGFKYNPPSGGPADTAVTRWIEERANALLAAALKGVARVSWEQAQRA
                     ATLQRHDYIDGYVRDLGAVIDMEAIASARLNIGVDPLGGAAVAYWRPIAERYGLALEV
                     VNDTVDPTFRFMPLDWDGKIRMDCSSPYAMARLIALRERFDIAFANDTDADRHGIVTR
                     SAGLMPPNHYLAAAIAYLFAHRSQWRSDAGVGKTVVSSAIIDRVARRAGRRLYEVPVG
                     FKWFVAGLLDGTLGFGGEESAGASFLRRDGAVWTTDKDGIILNLLAAEITAHTRLDPA
                     QLYQQLTEELGAPAFARTDTPATKEEKAALLRLSAADIRTTQLAGDPIEQMLVNAPGN
                     GAAIGGLKVITASGWFAARPSGTEDIYKLYAESFRGPEHLQRIQSEARALINTALAAA
                     IGAKR"
     gene            2827..>3291
                     /locus_tag="VI195_11465"
     CDS             2827..>3291
                     /locus_tag="VI195_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY6825052.1"
                     /translation="MSTRAPTFKASPAEAIRTGLAAGTLQRAVLDNLLYLQARLRPLA
                     TPQDWYMALAYSVRDRMLARWESTARVCARRDAKVACYLSAEYLIGPQLGNNLVNLGI
                     QLSARTAMAALDQDLDTLIALEGEPGLGNGGLGRLAACYLDSLATLEVPAIGY"
BASE COUNT          590 a         1092 c         1083 g          526 t
ORIGIN      
        1 cggccgggtg atggaggtgc tttccgagca taccctgatg ggttggctcg agggttacct
       61 gctcaccggg cgccacggat tcttcgccac ctacgaggcg ttcgcgcacg tcgtcgactc
      121 gatgttcaac cagcacgcca aatggctgga catcagcaag aaccacgtgc gctggcgcgc
      181 cgcggtcgcc tcgcagaaca tcctgctgac ttcgaccgtg tggcgccagg accataacgg
      241 cttctcgcac caggatccgg ggttcatcga tctcgcgacc aacaagagcc cctcggtgac
      301 acgggtatat ctgccgccgg atgccaatac gctgctggtg gtggcggacc gttgcctgcg
      361 ttcgaccgat tgcatcaacg tgatcgtggc cgacaagcag aagcatctgc agttcaccac
      421 catcgagcag gcaatcgtcc attgtgccaa gggcgtcggc atcatacggg ccgtgagcaa
      481 cgatgagggc tcggagcccg atgtggtgat ggcgtgctgc ggcgatgtcg ccacgcagga
      541 agcgttggcg gccacctcca tcctgcgcga gcgactgccg gagctcaagg tgcgcttcgt
      601 gaacgtagtg gaccttttca agatgcagcc ggcgagcgag cacccacacg gatccagcga
      661 gcgcgagttc gacagcctct tcacccgcga caagccggtg atcttcaact tccacggcta
      721 cccgtggctg attcacaagc tgtgctaccg cttcaagaac agcgagaaca tccacgttcg
      781 cggctacaag gagaagggca acatcaacac cccgctcgag ctcgccatcc tgaaccaggt
      841 cgaccgcttc gatctggtga tcgacgtcat cgatcgcgtg ccgggcctcg ccgagcgcgc
      901 ggctcacctg aaggaggaca tgaagaacgc catcatcgac agtctcacct atgcgcaccg
      961 cgagggcgcg gaccgagcag aggtcgcgaa ctgggtatgg ccgggatgaa cggcaagccg
     1021 gaggcgggca gcggccggca cgaggatgcg acctggccga gcggcgttca tccgctcgcg
     1081 ggcaagcctg cgccggcgag cgtgctgatc gatccggaca agctgtgccg ggagtactac
     1141 gagcgtcgac ccgacagcgc cgatccagcg cagcgcgtca gcttcgggac cagcggccac
     1201 cgcggctcgg cgctgcacgg ttcgttcaac gagttccacg tgctcgcaat cacgcaggcg
     1261 atctgcgagt accgcagcaa ggagtcgatc accggcccgc tgttcatcgc gcgggatacg
     1321 catgcgctct ccgggccggc gttgcgcacc gccctggagg tgctggcggc caacgacgtg
     1381 cagacgatga tcgatcgaag cgacggttac acgcccacgc ccgtgctgtc gcacgccatt
     1441 ctcacccaca accgcgcacg gcctgcgcgg caggccgacg gcatcgtcat cactccctcg
     1501 cacaatccgc ccgaggacgg tggtttcaag tacaacccac cgagcggcgg cccggccgac
     1561 accgcggtga ctcgctggat cgaggagcgc gcgaatgcgc tgctcgccgc tgcgttaaag
     1621 ggcgtggcgc gagtgtcctg ggagcaggcg cagcgcgccg cgacgctgca gcggcacgac
     1681 tacatcgatg gctacgtgcg cgatctcggt gcggtcatcg acatggaggc gattgccagt
     1741 gcacggctca acatcggcgt cgatccgctc ggtggcgcag cggtagccta ctggcggccg
     1801 atcgccgagc gctatggact tgcgctcgag gtggtgaacg ataccgtcga tcccaccttc
     1861 cgcttcatgc cgttggactg ggacggcaag atccgcatgg actgctcctc gccctacgcc
     1921 atggcacggc tcatcgcgct gcgcgagcgc ttcgatatcg cctttgccaa cgacaccgac
     1981 gccgaccggc acggcatcgt gacgcgcagc gcgggcctca tgccacccaa ccactacctg
     2041 gcggcagcga tcgcctacct cttcgcccac cgttcgcagt ggcgcagcga cgcgggggtg
     2101 ggcaagacgg tcgtgagcag cgccatcatc gatcgggtgg cgcggcgcgc gggtcgccgg
     2161 ctctacgagg tcccggtggg attcaagtgg ttcgtggcgg gactactcga tggcacgctc
     2221 ggttttggcg gtgaggagag tgccggtgct tcgttcctgc gccgggatgg ggcggtgtgg
     2281 accacggaca aggacggcat catcctcaat ctcctggccg cagagatcac cgcgcacacg
     2341 cggctcgatc cggcacagct ctaccagcaa ctcaccgagg agctcggcgc gcccgccttc
     2401 gcgcgcaccg atacccctgc caccaaggag gagaaggcgg cacttctcag gctttcggcc
     2461 gccgacatcc gcaccacgca gctcgccggc gatcccatcg agcagatgct ggtgaatgcg
     2521 ccgggcaacg gagccgccat cggtggcctc aaggtgatca ccgcgagcgg ttggttcgcc
     2581 gcgcgaccct cgggcacgga agacatctac aagctgtatg cggagagctt ccgcgggccg
     2641 gaacacctgc aacgcatcca gtcggaggcg cgggcgctca tcaatacggc gctggcggcg
     2701 gcgatcgggg caaagcgcta ggacactgcc gttcccgagc gcgcgtggtg aacgcgtcca
     2761 tggctgccca ggatgtcttt ctgatccggc atggcgagac cgaatggagt ctcaggaggg
     2821 cagcctatga gcacgagagc gccgaccttc aaggcttcgc ctgctgaagc catccgcacc
     2881 ggacttgctg cgggcacgct gcagcgtgca gtgctcgaca acctgctcta tctgcaggcg
     2941 cggctccggc cgcttgccac accacaggac tggtacatgg cgctcgcgta cagcgtgcgc
     3001 gaccggatgc tggcgcgctg ggagagcacg gcgcgcgtct gcgcgcggcg ggacgcaaag
     3061 gtcgcctgtt atctgtcggc agagtacctc atcggtccgc agctcgggaa taacctggtc
     3121 aacctcggca ttcagctaag cgcgcgcacc gcgatggcgg cgctcgatca ggacctcgat
     3181 actctgatcg cgctggaagg ggagcccgga ctcggcaatg ggggactcgg gcggctcgcg
     3241 gcctgctacc tcgactcgct ggcaacgctc gaggtgccgg cgatcggtta c
//
LOCUS       DATDMP010000654         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_991489, whole genome shotgun sequence.
ACCESSION   DATDMP010000654 DATDMP010000000
VERSION     DATDMP010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991489"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VI195_11470"
     CDS             complement(<1..581)
                     /locus_tag="VI195_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825053.1"
                     /translation="MTVSIARPAAPRGTGLGLAIAALTGFALLAGAGIALGELEAMYG
                     VLSLIAAAAVLADFRFGAVLLILLMPVEKSYYFPHSLFGVTGVNPLNVVLLATLASFV
                     LRGKGLSRFMPKPLFLMVVVPVLFAGLLGLQHVDEIHPGFYEAMLISFNNGFGYFRDV
                     AVKPLFIVLNACIIGAAVARSQKPERFLVPIILS"
     gene            complement(583..735)
                     /locus_tag="VI195_11475"
     CDS             complement(583..735)
                     /locus_tag="VI195_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825054.1"
                     /translation="MRRPPDRKLALFVMAAITIAAASSVLASRTVAGPGEASPAALIA
                     KSLGRS"
     gene            complement(736..1623)
                     /gene="epsG"
                     /locus_tag="VI195_11480"
     CDS             complement(736..1623)
                     /gene="epsG"
                     /locus_tag="VI195_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HEY6825055.1"
                     /translation="MLVKPSDNVLPMDGVARPGGRQIGAILMDEGKLTPSDAEQVLKR
                     QRELGWRFGEAAIELNLITDTDLREALAKQYEFPYLVGGPEGVAKELIAAWDPFHPVV
                     EELRAVRTQLLIRWFNPQAGRRTLAIASPGAREGRSFVAANLAVVFSQLGQRTLLIDA
                     DFRAPRQQSIFNIADRFGLSSVLSGRADLSAAVPVAGLTGLAVLPCGPVPPNPLELLS
                     RGSFAALLNKAQAEYDLILIDTPPASEYADAQSIAFRAGDVLLLSRRNQTRVDDTERA
                     VRELSDASARIVGTLMNSF"
     gene            complement(1640..>2158)
                     /locus_tag="VI195_11485"
     CDS             complement(1640..>2158)
                     /locus_tag="VI195_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825056.1"
                     /translation="LIASLGNAAAQAKRREQELTTALAAQQDRIMKAKDARIEMAVLT
                     RDLDSSQRAYDGALQRYIATKVESRARQTNLAMLTPAVEPLKPAVPNVPLISGLAVLI
                     GVLLAAGVVYLLEMADRRVRSRGDLEQTLAVPSLGNLSKWLPTGGRLLPSPSYSGAHT
                     GSGAPRALPHPW"
BASE COUNT          336 a          718 c          802 g          302 t
ORIGIN      
        1 gagaggatga tcggcacgag gaagcgctcc ggcttctgcg agcgcgcgac ggccgcgccg
       61 atgatgcacg cgttgagcac gatgaacagc ggcttcaccg ccacgtcccg gaaatagccg
      121 aacccgttgt tgaagctgat cagcatcgcc tcgtagaagc cgggatggat ctcatcgacg
      181 tgctgcagcc ccagcaggcc ggcgaacagc accggcacca cgaccatcag gaacagcggc
      241 ttgggcatga agcgcgagag gcccttgccg cgcagcacga aggaggcgag cgtcgcgagg
      301 agcaccacgt tgagcgggtt tacgccggta acgccgaaca ggctgtgcgg gaagtagtag
      361 ctcttctcga ccggcatcag caggatgagc agcacggcgc cgaagcggaa gtcggcgagc
      421 accgccgccg ccgcgatcag gctcagcacg ccgtacatcg cctcgagctc gccgagggcg
      481 atgccggctc ccgccagcag cgcgaacccg gtgagcgcgg caatggcgag tcccaggccc
      541 gtgccgcgcg gggcggccgg gcgtgcaatc gacacggtca tgtcaggacc ttccgagaga
      601 tttcgcgatc agcgccgcgg gcgacgcctc gcccggtccg gcgacggtgc gggaggcgag
      661 cacgctgctg gcggcggcga tggtgatcgc cgccatgacg aacagcgcga gcttgcgatc
      721 cggcggccgc cgcattcaga acgagttcat cagcgtgccg acgatgcggg cactggcgtc
      781 ggagagctcc cggaccgcgc gctcggtgtc gtcgacccgc gtctggttgc ggcgcgagag
      841 cagcagcacg tcgccggcgc ggaaggcgat gctctgcgcg tccgcgtact cgctcgccgg
      901 cggggtgtcg atgaggatca ggtcgtactc ggcctgcgcc ttgttgagca gcgcggcgaa
      961 gctgccgcgc gaaagcagct cgagcgggtt cggcggcacc ggtccgcacg gcagcacggc
     1021 caggccggtc aacccggcca ccggcaccgc ggccgacagg tcggcacgcc cggagagcac
     1081 cgaggacagg ccgaagcggt cggcgatgtt gaagatcgat tgctgccgcg gggcgcggaa
     1141 gtcggcgtcg atcagcagcg tgcgctggcc gagctgcgag aacaccacgg cgaggttggc
     1201 cgcgacgaag ctgcgcccct cgcgagcgcc ggggctcgcg atggcgagcg tgcgccggcc
     1261 cgcctgcggg ttgaaccagc ggatgagcaa ttgcgtgcgc accgcgcgca gctcctcgac
     1321 caccggatgg aacggatccc aggcggcgat cagctccttc gccacgccct cgggtccgcc
     1381 caccaggtag gggaactcgt actgcttggc gagcgcctcg cgcaggtcgg tgtcggtgat
     1441 caggttcagc tcgatcgccg cctcgccgaa gcgccagccg agctcgcgct ggcgcttgag
     1501 gacctgctcg gcgtccgaag gcgtgagctt gccttcgtcc atgaggatgg cgccgatctg
     1561 gcggccgccg ggccgcgcta cgccgtccat cggcagcacg ttgtcggagg gtttgactag
     1621 catggcagtc ctctcttgat taccagggat gcggcagggc gcggggtgcg ccgctaccgg
     1681 tgtgggcgcc ggaatagctg ggcgagggca gcaggcggcc gccggtgggc agccacttgg
     1741 acaggttgcc gagcgagggc acggcgagcg tctgctcgag gtcgccgcgc gagcgcacgc
     1801 gccggtcggc catctcgagg agatagacga cgccggcggc gagcagcacg ccgatcagca
     1861 cggcgagccc gctgatcagc ggcacgttcg gcaccgccgg cttcagcggc tcgaccgcgg
     1921 gcgtgagcat cgccaggttg gtttgccgcg cgcggctttc caccttggtg gcgatgtagc
     1981 gctgcagcgc gccgtcgtag gcccgctgcg agctgtcgag gtcgcgcgtc agcacggcca
     2041 tctcgatgcg cgcgtccttc gccttcatga tgcggtcctg ctgcgcggcg agcgcggtgg
     2101 tgagctcctg ctcgcgccgc ttcgcctgtg ctgcagcgtt gccgaggctc gcgatcag
//
LOCUS       DATDMP010000655         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_547947, whole genome shotgun sequence.
ACCESSION   DATDMP010000655 DATDMP010000000
VERSION     DATDMP010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547947"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VI195_11490"
     CDS             <1..549
                     /locus_tag="VI195_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY6825057.1"
                     /translation="VGQTWNLAATAVWLKILGEIDTSPTEITSRLRLPYQRHVAGAWR
                     DAASAWAELGCPYEQAIALAAGDEAGRREALALFDKIGAIPAAARLRRQMRASGTRSV
                     PRGPIGKTRANPAGLTGRQAQVFALLVKGLSNPQIAHRLNISPKTAEHHVSAIIARLG
                     VTTRAEAAAAARARGLADTLES"
     gene            639..1406
                     /locus_tag="VI195_11495"
     CDS             639..1406
                     /locus_tag="VI195_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825058.1"
                     /translation="MSRIGIFVYGVLCYALCLVAFVYAIGFIGGFFTPTMLDGPAQRP
                     LGAAFTVDFALLVAFALQHSIMARPAFKRWWTDIVPEAAERSTYVLASSIAVGALFAF
                     WEPMGGVVWQAAGAVRTAVIGVYLFGWALLLYVTFLIDHFDLFGLKQVWHRLTGKKYR
                     PPQFYTPSLYKLVRHPLYIGWLAIFWAAPTMTVSHLVFALVTTAYILFAIRLEERDLV
                     LAFGADYVAYRARTPMLVPQLPVLGTGGVAAKSRART"
     gene            1409..2992
                     /locus_tag="VI195_11500"
     CDS             1409..2992
                     /locus_tag="VI195_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY6825059.1"
                     /translation="MRGNADDWPVGGPTVTDTRPAPTADGHRRLFQRAAYHASAFRDR
                     VADRAPCPAISAAALQARFDGPTPEVGQDPLAVLDALQSAAEPGLTGAAGPRFFGWVI
                     GASDPLAVAADMLTSAWGQNAAAYACAPAAAMAEKVAARWLLDILQLPQECSVGFVTG
                     ATMAGFVCLAAARSAALAQVGWDVEADGLAGAPAVRVFVGEDAHVTTLAALRYLGFGS
                     RALRVPSDAQGRMNAPALRTALALGSGPAIVIAQAGQVNTGDFDAVGELAHICRKRGA
                     WLHVDGAFGLWARTVPELAQLTAGLEEADSWSVDGHKWLQLPYDSGFAIVRDPDAHRR
                     AMSMTASYLPPAGESEYDGGQFVPELSRRARGFAVWAQLRALGRHGIAELVRRHCELA
                     RRLARRLASAPGVTVLNSVPLNQVIVSFGTGTLEECNVLTRATVAQLRADNICLAGGA
                     EWRQHFVLRLSLIAAPLVEADVDRLAAAILAAWRRVQSRAREHQQTPSPQLTPSAASS
                     GTRVTGATNSSALPASTLK"
BASE COUNT          473 a         1015 c         1038 g          525 t
ORIGIN      
        1 gtcggccaga cctggaatct ggccgcgacg gccgtctggc tcaagatact cggggaaatc
       61 gacacgtcac cgacagagat cacgagccga ctgcgtctgc cctatcagcg acacgtcgcg
      121 ggcgcgtggc gcgacgcggc cagcgcctgg gccgagctcg gttgcccgta cgagcaggcg
      181 attgcactcg cggcgggcga cgaggcgggg agacgtgaag cgctcgcgct cttcgacaaa
      241 atcggggcca ttcccgccgc ggcacggctg cgacgtcaga tgcgcgcgag cggcacgcgt
      301 tccgtcccgc gcggcccgat cggcaagacc cgcgccaacc ccgcaggact gacaggtcgt
      361 caggcgcagg tgttcgccct actcgtcaaa ggcctcagca atccccagat agcccaccgc
      421 ctgaacatct ctccaaagac ggccgagcat catgtgtcgg cgatcatagc gcgcctcggt
      481 gtcacgacac gggcagaagc cgccgcggcc gcacgagcgc gcggactcgc cgacaccctc
      541 gaaagttagg ggtagctgct accagagatt ggggacgcgc cccatgcacc tcccctacac
      601 cgcggccatg ctgccacctc gcaaatcagg aggcagtgat gtcacgcatc ggaatattcg
      661 tctacggagt cctgtgttat gcgctgtgcc tggtggcgtt cgtctatgcc atcggattca
      721 tcggcggctt tttcacgccg acgatgctcg atggaccggc acagcgtcca ctcggcgccg
      781 cctttacggt ggattttgca ctgctcgtgg cgttcgcgct gcagcacagc atcatggcgc
      841 ggccggcgtt caagcgctgg tggacggaca tcgtcccgga ggcggccgaa cgcagcacct
      901 atgtgctcgc cagctcgatt gcggtcgggg cactgtttgc gttctgggag ccaatgggcg
      961 gtgtggtgtg gcaagccgcc ggggcggtgc gcaccgcggt catcggcgtg taccttttcg
     1021 gctgggcgct gctgctgtat gtgaccttcc tcatcgacca tttcgacctc ttcggcctca
     1081 agcaggtgtg gcacaggctc accggcaaga agtaccgacc gccgcagttc tacacgccga
     1141 gcctgtacaa gctcgtccgg catccgcttt acatcggctg gctggcgatc ttctgggccg
     1201 caccaaccat gacggtctcg cacctggtct tcgcactggt gacgaccgcg tacatcctgt
     1261 tcgcaatccg gctcgaggaa cgtgacctgg tgctggcatt cggcgccgac tacgtggctt
     1321 accgtgcgcg tactccaatg ctcgtgccac agctgccggt gctcgggacc ggcggtgtcg
     1381 cggcgaagag ccgggcacgg acctgaacat gcgcgggaat gctgatgact ggcccgtcgg
     1441 cggtccgaca gtgaccgata cgagacctgc gcccactgcc gacggccacc gtcgtttgtt
     1501 ccagcgcgcg gcttaccacg cgagtgcgtt ccgcgatcga gtggcggacc gcgcgccgtg
     1561 cccggcgatc tcggcggcag cgctgcaggc gcgcttcgat gggccgacgc ccgaagtcgg
     1621 ccaagatccc ctcgcggtcc tcgacgccct gcagtcggca gcggagccgg gcctcaccgg
     1681 cgccgccgga ccgcgtttct tcggctgggt gatcggtgcg tcggatccgc ttgccgtggc
     1741 ggccgacatg ctcacgagcg catggggcca gaacgccgct gcctatgcct gcgcgccagc
     1801 ggccgccatg gccgagaagg tcgcggctcg ctggttgctc gacatcctgc aactgccgca
     1861 ggagtgctcg gtcggattcg tcaccggtgc cacgatggcg gggttcgtgt gtctggcggc
     1921 tgcgcgcagc gccgcgctcg cgcaggttgg ctgggacgtg gaagccgatg ggctcgcggg
     1981 cgcaccggcg gtgcgcgtgt tcgtgggcga ggacgcgcac gtcaccacac tcgccgcgct
     2041 gcgctatctc ggttttggca gtcgtgccct gcgcgtgccg agcgacgcac aaggccgtat
     2101 gaatgccccg gcactgcgca cggcgcttgc actcggcagc gggccggcca tcgtgatcgc
     2161 gcaggcgggt caggtcaaca cgggcgattt cgatgccgtg ggcgagctgg cccatatctg
     2221 ccgcaagcgc ggagcctggc tgcatgtcga tggcgcgttt ggactttggg cacgcacggt
     2281 accggagctg gcgcagctca ccgcgggact cgaggaagcg gactcctggt cggtcgacgg
     2341 gcacaagtgg ctgcagctgc catacgacag cggattcgcc atcgtcaggg accccgatgc
     2401 tcaccggcgt gcgatgtcca tgacggcgag ctatctgccc ccagccggtg agtcggagta
     2461 tgacggcggc cagttcgttc cggaattgtc ccggcgcgcg aggggcttcg ccgtgtgggc
     2521 acagttgcgg gcactaggtc gtcacggcat tgccgagctg gtgcgccgtc actgcgagct
     2581 ggctcgccgg ctcgcccggc gccttgcgag tgcgcccggc gtgaccgtgc tcaactccgt
     2641 tccgctcaat caagtcatcg tgagcttcgg cactggcacg ctcgaggagt gtaacgtgct
     2701 cacgcgtgcc accgtggcgc agctgcgggc ggataacatc tgcctggcgg gcggagcaga
     2761 gtggcgccag cacttcgtgc tgcggctgtc gctgattgcc gcgccgctgg tcgaagccga
     2821 cgtggaccgg ctcgccgcag ccatcctggc ggcgtggcgc cgtgtccaga gtcgggcacg
     2881 tgagcaccag cagacgccct cgccacagct cacaccgtct gccgcaagtt ctggcacgcg
     2941 cgtcacgggt gccactaaca gcagcgctct tccagcttcg acgctaaaat gacaagaggg
     3001 tgaggtatcg aagctcggaa ccctaaggca atttcagggg cagatgatgc t
//
LOCUS       DATDMP010000656         3927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1105017, whole genome shotgun sequence.
ACCESSION   DATDMP010000656 DATDMP010000000
VERSION     DATDMP010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105017"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VI195_11505"
     CDS             <1..391
                     /locus_tag="VI195_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825060.1"
                     /translation="AVIWRDQAVEARSKAFATRADMLFAEGMRADPYQVANYIERARM
                     HRTHAELFDQPAGPRQLLAWTGEALRLRPFSLVAQAEHARTLAYAGQTEEALRIVRAM
                     LERHGDTGMARGLAKEFGLREPVETTR"
     gene            388..852
                     /locus_tag="VI195_11510"
     CDS             388..852
                     /locus_tag="VI195_11510"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY6825061.1"
                     /translation="MSRERSDAAGFTLTELAVVIAIATILSAFAISRINTASFDTEGY
                     ANQVKAAIRYAQKIAVSQRRNVAVTVSAGAVSLTYPDAPLSGAPVHRPPGTDAFTVNK
                     PTSVTLSGTLSGSSVTFSALGKPVAGGGTIIVSGGDISPITITVESETGYVH"
     gene            842..1441
                     /locus_tag="VI195_11515"
     CDS             842..1441
                     /locus_tag="VI195_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6825062.1"
                     /translation="MSIRQSRAICSGVTLVELIVFIVIVGVALAGLFAAFNTMTAGSA
                     DPQVRKQVLAIAESLLDEVALMPLTYCDPDDANAATATNGAGCTGGAGGANDEDKSPL
                     GPESTEDRYGTTLPFDNVSDYNGFVMPAPPGIKDINGNVISGLEGYSAQITVTRAGTA
                     LGLAADGDALLISVQVTGPTGVSTTLEGYRMRYAPNTLP"
     gene            1438..2337
                     /locus_tag="VI195_11520"
     CDS             1438..2337
                     /locus_tag="VI195_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6825063.1"
                     /translation="MTMRTSRGNFTRSSGVTLIELAITIALVGILAALIVQFVSPVRS
                     YIDTSRRAALADTADTALRRIGRDLRLALPNSVRVTTSGGVVYLEFLLVRTAGRYRFE
                     AGTGTDCGGTTADDALVFGAADTCFTSIGGILNRSEVVSTDYLVVFNLQPGTDKGDAY
                     QTTCGSACNKSQISATLPAVGTDGDVIKFASNTFTYESPGRRFFIIEGPVTYACDVSG
                     GTLKRYSGYGIAFTQPTPPGGSPALIASGVNGCSFTYDNVAGAANQGAGLVTMRLQLR
                     AQSSDGANEDVTLYHAVHVSNVP"
     gene            2334..2813
                     /locus_tag="VI195_11525"
     CDS             2334..2813
                     /locus_tag="VI195_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825064.1"
                     /translation="MSASIRRQRQRGFLVIAAVFLLVVLAGLVGYLMTVSTTSQAASA
                     ADANSARAYQAARGGIEWATYQVLRNSGGSFVTTTCTGGAPTKVNVNFGSTLSSFTAT
                     VTCSRVPSITEGGASVTVYTIVSNACNELVGSPSACPNTSTVSSTYVERQLSITLTN"
     gene            complement(2832..3830)
                     /locus_tag="VI195_11530"
     CDS             complement(2832..3830)
                     /locus_tag="VI195_11530"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HEY6825065.1"
                     /translation="MSTSSTPQFQTFGAADSACQTPTAGSKRSFTYVGQSFKYLSTAV
                     PVATFTGQNAANGTLANYVGSLVHASGISAVQTYTPNSATLTATVGSPTIAAIADPGP
                     GGTVTINSGDSFAYVRSNTTPQAAFSANISLDVTVSDTSEAGASQGTITTNSAGTFSA
                     IAFDSGNLFRYGRLRILGAAGSEEIPLPVPVQTEYWNGTGFVLNGDDHCTTLAQSNVA
                     QGNYQKSLNACETVASTATVSFVGGAGRLTLTKPGAGNNGSVDLSPQLGASIVVGSKY
                     CATAGGAEAAATAASKSFLQGAWGGATTYDQNPSARASFGVYGAQPRNFIFRRENY"
BASE COUNT          677 a         1269 c         1277 g          704 t
ORIGIN      
        1 agcagtcatc tggcgcgacc aggcagtgga ggcgcgtagc aaggcgtttg ccacacgggc
       61 ggatatgcta tttgccgaag gtatgcgcgc agatccttac caagtcgcca actacatcga
      121 acgtgcacgg atgcatagaa cgcacgccga actgtttgac cagcccgcgg gtccgcggca
      181 gttgctggcc tggaccggcg aagcgctgag acttcggccc ttttcgctag tcgcgcaagc
      241 ggagcatgca cgtacgctcg cctacgcggg gcaaaccgaa gaggccctcc gcatagtgcg
      301 cgcgatgctc gaaaggcatg gcgataccgg catggcgcgc ggcctggcaa aggaattcgg
      361 cttgcgggag ccggtggaga cgacccggtg agccgcgagc gttcggatgc agcgggattc
      421 acgctcaccg aattggccgt agtgatcgcg atcgcaacca tcctctccgc gtttgccatt
      481 tcgcgaatca acaccgccag cttcgacacc gagggctatg ccaaccaggt gaaagcggcg
      541 atccgctatg cgcagaagat cgccgtcagc cagcggcgca atgttgccgt cacggtgtct
      601 gcgggtgcag tgtcattgac ctatccggat gcgcctctga gcggtgctcc ggtgcaccgg
      661 ccgcctggta cggacgcgtt cactgtgaac aagccaacca gcgttacttt gtcggggaca
      721 ctgtctggaa gttccgtcac cttcagcgcg ctcggcaagc cggttgcggg cggcggcacg
      781 atcatcgtga gcggtggcga tatctcgccg atcaccatca ctgtcgagtc ggagaccggc
      841 tatgtccatt agacaaagcc gggcaatctg cagcggcgtc acgctcgtcg agctcatcgt
      901 cttcatcgtc atcgtgggcg tggcgctggc cggattgttc gccgctttca acaccatgac
      961 agcaggcagc gccgatccgc aagtgcgcaa gcaggtgctc gcgatcgccg agtcgctact
     1021 ggacgaggtg gcgctcatgc cgctcactta ctgcgatccc gatgatgcga acgccgctac
     1081 agcgacgaac ggcgcgggct gcaccggcgg tgcgggcggt gccaatgacg aggacaagtc
     1141 gccgcttggc ccggaatcca ccgaggaccg ctacggcacc acgctgccct tcgacaacgt
     1201 gagcgactac aacggcttcg tcatgcccgc gccgcccggg attaaggaca tcaacggcaa
     1261 cgtgatcagc ggtctggagg gctactccgc gcagatcacg gtgacgcgcg ccggcacggc
     1321 cctgggactg gcagcggacg gcgatgcctt gctcatcagc gtgcaggtga ctggcccgac
     1381 cggcgtttct acgacgctcg agggctaccg catgcgctac gcgccgaata cgctgccatg
     1441 accatgcgca ccagccgcgg caatttcacg cgcagcagtg gtgtcacgtt gatcgagctt
     1501 gcgatcacga tcgcgctggt gggcatcctc gccgctctga tcgtccagtt tgtttcgccg
     1561 gtccgctcct acatcgatac cagccgccgg gcggctctgg ccgatacggc agacacggcg
     1621 ttgcgccgca tcggacgcga cctgcggctc gcgctgccga acagcgttcg cgtcacgacc
     1681 tccggcgggg tcgtgtatct cgaattcctg ctggtgcgca cggcaggacg ctaccgcttt
     1741 gaggcaggca ccggaaccga ttgcggcggt acgaccgcgg acgatgcgct ggtgttcggc
     1801 gcggcggaca catgcttcac gtcgattggc ggcattctca accgctccga agtggtctcg
     1861 acggactatc tggtggtgtt caatctgcag ccgggtacgg acaagggcga cgcgtatcag
     1921 acgacctgcg gcagcgcatg caacaagagc cagatctccg cgaccttgcc ggcggtcggt
     1981 accgacgggg acgtcatcaa attcgcgtcc aacaccttca cctacgagtc ccctgggcgg
     2041 cgcttcttca tcatcgaggg ccccgtaacc tacgcttgcg atgtgtctgg cggcacgctg
     2101 aaacggtatt cgggctacgg cattgctttc acgcagccga cgccccctgg tggcagcccg
     2161 gcgctgatcg cgagcggcgt caacggctgc agcttcacct acgacaacgt cgcgggcgcc
     2221 gcgaaccagg gcgccgggct ggtgacgatg cgactgcagc tgcgtgcgca gtcctcggac
     2281 ggcgcgaacg aagacgtgac cctgtatcac gcggtccacg tgagcaacgt cccgtgagcg
     2341 catccatccg caggcagcgg caacgcggct ttctcgtcat cgccgcggtg ttccttctgg
     2401 tcgtcctcgc gggcctggtc ggctatttga tgaccgtgtc cacgacgtcg caggcggcgt
     2461 cggcggccga cgccaattcg gcgcgcgcgt accaggcggc gcgcggcggc atcgaatggg
     2521 cgacctacca ggtgctgcga aattccggcg gctcgttcgt gacgaccacc tgcaccggcg
     2581 gcgcgccaac gaaggtgaac gtgaactttg gttccacgct gtcaagcttt accgcgaccg
     2641 tcacctgcag ccgggttcca tcgatcaccg aaggcggtgc aagcgtcacc gtctatacca
     2701 tcgtctcgaa cgcctgcaat gagctggtcg gctcgcccag cgcatgcccc aatacctcca
     2761 cggtttcgtc aacctacgtc gagcgccagt tgagcatcac cctgacgaac tgagcgcgcg
     2821 cggcctccct ctcagtagtt ttcccggcgg aagatgaaat tgcgcggctg cgcgccgtat
     2881 accccgaagg aggcgcgtgc cgacggattc tggtcgtagg tcgtagcgcc gccccatgcg
     2941 ccctgcagga aactctttga cgccgcggtg gccgcggctt ccgcgccccc ggcggtcgcg
     3001 caatatttgc tgccaacgac gatgctcgcg cctagctgcg ggctcaggtc gaccgacccg
     3061 ttgttcccgg ctcccggctt ggttagcgtc agcctgcccg cgccgccgac gaaactcacc
     3121 gtggccgtgc tggcgacggt ttcgcaagcg ttcaggctct tctggtagtt gccctgcgcg
     3181 acgttggact gcgcgagcgt cgtgcagtga tcatcgccat tgagcacgaa gccagtgcca
     3241 ttccagtact cggtctgcac cggcaccggc agcggtatct cctcgctgcc ggccgcgccg
     3301 aggatccgca ggcggccgta gcggaatagg ttgccggaat cgaaggcgat cgcggagaag
     3361 gtgcccgcgc tgtttgtcgt gatcgtgccc tggctggcgc cagcttccga cgtgtcggac
     3421 acggtgacgt cgagcgaaat gttcgcgctg aacgcggctt gcggcgtcgt gttgctgcgc
     3481 acgtaagcaa agctgtcgcc cgagttgatc gtcactgtac cgccgggccc ggggtctgcg
     3541 atcgcggcga tggttggtga accgacagtc gcggttagag tggccgaatt cggcgtgtag
     3601 gtttgcaccg cggagatccc ggacgcgtgc accaggctgc ccacgtagtt cgcgagagtg
     3661 ccattggcgg cgttctgccc ggtgaatgtc gcgaccggga ctgcggtcga gagatacttg
     3721 aacgactggc cgacgtaagt gaagctgcgc ttgctgccgg cggtcggcgt ctggcatgcg
     3781 ctgtcggcgg cgccgaaagt ctggaactgc ggcgtcgagc tcgtcgacat cacgaagtga
     3841 tcgggcacga agcgccccac attgaacgcc gccgaggaaa aatagcgctc ggccgtactc
     3901 gatccatcag agttgtccac ggacgac
//
LOCUS       DATDMP010000657         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_437510, whole genome shotgun sequence.
ACCESSION   DATDMP010000657 DATDMP010000000
VERSION     DATDMP010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437510"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VI195_11535"
     CDS             <1..529
                     /locus_tag="VI195_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6825066.1"
                     /translation="VLDINMPGLSGFQVCEAIRARSRVPVMMLTVRGEEEDLVRALGL
                     GADDYLTKPFSPRTLLARIRALLRRAGMESTAPVAAGRIALDLSEHTVRIGDAEPVRL
                     TKLELRLLQMLLANAGHTVSSERLLVQVWGHRSGGDRQLLKQLVHRLRQKIEGDPAAP
                     ALLRTAAAGYKLVVD"
     gene            complement(508..1539)
                     /locus_tag="VI195_11540"
     CDS             complement(508..1539)
                     /locus_tag="VI195_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6825067.1"
                     /translation="MVREAGNGRNFTWVLILISLCSASASMATEGDATTLRVNVLYDA
                     FGKASGLTKDWGYAALIDYGGKRILFDTGNNPDILARNARASGVDLSRLDLVILSHRH
                     ADHMGGMEYLLAVNPNVKIYAPKENFGIYGSSLPGTFYRRDASLAPEQRYYDGSPPAT
                     MVFGSAWPRAHFELIDGATEIAPGLHLIALVSDKPGTLELRELSLAIETSDGLVLVVG
                     CSHPGIENIVQAAASIDHRIHLIVGGLHLVTANDADIAKTVTSLHDTWKVDYIAPGHC
                     TGEATFTALRKAFGDHYLSAGLGTRLSVGRVPTVAAITAATVNPGGRSTLRAGADSAR
                     GPSQSTTSL"
     gene            complement(1575..>2608)
                     /locus_tag="VI195_11545"
     CDS             complement(1575..>2608)
                     /locus_tag="VI195_11545"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6825068.1"
                     /translation="STYPTLRICARVLVTLALAALISCGKGASGAGGSRITLRNVSYD
                     PTRELYVEFNAAFARYWQGKTGQRVRVEQSHGGSGTQARSVIEGNAADVVTLALAADI
                     DAIAATGRLLPVNWQSLLPDNSSPYTSTVVFVVRKGNPKGIRDWGDLVRPGVAVITPN
                     PKTSGGARWNYLAAWAWARAQPGGDDARAREFLRRVYANVPVLDAGARGATTTFVHNG
                     LGDVLLTWENEAMLAVREVGADKLEIVVPSVSVLAEPPVAVVERVALRHGTREVARAY
                     LDYLYSKEGQEIIARHYYRPRDPEVAARYAERFPQLKLATVADFGGWASVQRTHFSDG
                     GVFDQISAR"
BASE COUNT          442 a          852 c          883 g          431 t
ORIGIN      
        1 ggtgctcgac atcaacatgc cgggcctctc cggcttccag gtgtgcgagg cgatccgtgc
       61 gcgctcgcga gtgccggtca tgatgctcac ggtgcgcggt gaggaggagg atctggtgcg
      121 cgcgctcggt ctgggcgccg atgactatct caccaagccc ttcagcccac gcaccctgct
      181 cgcgcgaatc cgcgcactgc tgcgccgcgc cggtatggag agcaccgccc cggtggcggc
      241 cggccggatc gcgctcgacc tgagcgagca caccgtgcgc atcggcgatg ccgagccggt
      301 gcgactcacc aagctcgagc tgcggctgct gcagatgctg ctggcaaatg ccggccacac
      361 cgtgagctcg gagcggctgc tggtgcaggt gtggggccac cgcagcggcg gggaccggca
      421 gctgctcaag cagctggtgc atcgcctgcg tcagaagatc gaaggggacc cggccgcgcc
      481 ggcgctgctg cgtaccgccg ccgctggcta caagctggtg gtcgactgac tggggccgcg
      541 ggcagaatcc gcgccggcgc gcagagtcga gcgtccaccg ggattgacgg tcgcagccgt
      601 gattgccgcg acggttggta cgcgcccaac gctcagccgg gtgccgagcc cggcagacag
      661 gtagtgatcg ccaaaagcct tacgcagtgc ggtaaaggta gcttcgccag tgcaatgccc
      721 gggagcgatg taatcgacct tccaggtgtc gtgcaggctc gtcaccgtct tggcgatatc
      781 ggcatcgttc gcggtgacga gatggagacc gcccacgatc agatgtatgc gatgatcgat
      841 cgaggcagcc gcctgcacga tattctcgat gcctgggtgg gagcatccga cgaccagcac
      901 gaggccatcg gaggtttcga tagccagcga taattctcgc agctcgagcg tgccaggctt
      961 atccgagacg agagcaatga ggtgcaggcc cggagcgatc tccgtggcgc cgtcgatcag
     1021 ctcaaaatgc gcgcgcggcc acgcactgcc aaacaccatg gtcgctggcg gcgatccgtc
     1081 gtagtagcgc tgttccggcg ccaacgatgc atcacggcgg tagaaggtgc ccggcagaga
     1141 agagccgtag attccgaagt tttccttagg cgcgtagatc tttacgttcg ggttgacggc
     1201 caatagatat tccatgccgc ccatgtgatc cgcatggcgg tgggaaagaa tcaccaggtc
     1261 caagcgcgac aaatcgacgc cgctggcccg ggcgttcctg gcgagaatgt ccggattatt
     1321 gccggtgtcg aagagtatcc gtttgccgcc gtagtcgatt aaagcggcat aaccccagtc
     1381 cttggtcagg cccgatgcct tgccgaatgc atcataaagg acattcactc ggagcgtggt
     1441 tgcgtcgccc tcagtcgcca tgctcgcgga ggcgctgcac aagctgatga ggatcaacac
     1501 ccaagtgaag ttgcgaccgt tgcctgcttc tctgaccatg acgccccctg tgcgccgcgc
     1561 cggctcgagt gaggttagcg cgccgagatc tggtcgaaca ccccgccgtc gctgaagtgc
     1621 gtgcgctgca cgctcgccca gccgccgaag tcggcgaccg tcgccaactt cagctgcggg
     1681 aaacgctccg cgtagcgggc cgcgacctcc gggtcgcgcg ggcggtagta gtggcgcgcg
     1741 atgatctcct ggccttcctt gctgtagaga tagtcgagat aggcgcgcgc gacctcgcgc
     1801 gtaccgtgac gcagcgcgac ccgctcgacc accgcgacgg gcggctcggc cagcacgctc
     1861 accgagggca cgacgatctc gagcttgtcg gcgccgacct cgcgcaccgc caacatcgcc
     1921 tcgttctccc aggtgagcag cacatccccg aggccgttgt gcacgaaggt ggtggtcgcg
     1981 ccgcgcgcac cggcgtcgag taccggcacg ttcgcataca cgcggcgcag gaactcgcgc
     2041 gcgcgcgcgt catcgccccc cggctgtgcg cgtgcccacg cccaggcggc cagatagttc
     2101 cagcgggcgc cgcccgaggt cttcggattc ggggtgatca ccgccacgcc ggggcgtacc
     2161 agatcgcccc agtcgcggat ccccttcgga ttgcccttgc gcaccacgaa cacgacggtg
     2221 gaggtgtagg gggagctgtt gtccggcaac aggctctgcc agttcaccgg caggagtctg
     2281 ccggtagcgg cgatggcgtc gatatcggcc gcgagcgcga gcgtcacgac atccgccgcg
     2341 ttgccttcga tgaccgagcg cgcctgggtg cccgagccgc catgggactg ctcgacgcgc
     2401 acccgctggc cggtctttcc ctgccagtaa cgcgcaaatg cggcgttgaa ctccacgtac
     2461 agctcgcggg tcgggtcgta ggagacgttg cgcagcgtga tgcgtgagcc gcccgcgccg
     2521 ctggcgcctt taccgcagga gatcagtgcc gccagtgcca gggtgacgag gacgcgtgcg
     2581 cagatgcgca gagtcggata ggtcgatc
//
LOCUS       DATDMP010000658         4426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_881759, whole genome shotgun sequence.
ACCESSION   DATDMP010000658 DATDMP010000000
VERSION     DATDMP010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881759"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1458)
                     /locus_tag="VI195_11550"
     CDS             complement(124..1458)
                     /locus_tag="VI195_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY6825069.1"
                     /translation="MRRIRVRLFLLSLAFAAAPAAAEDLLQVYRDAKAYDAQYASAKQ
                     ALQAGLEKLPQGRALLLPTLGLTAATTTNHLELSPYGPTPVPAGTRPVYNRNYQLPLA
                     QPVFRAQNWAQYDQAEQQVRQSEATFGQASQDLILRVTQAYVDVLAAQDNLTLVNAQK
                     VAIGEQLAQAKRNFEVGTATITDTHEAQARYDLTTAQGIVAQNDVDTKIRALQQLTGK
                     VYTRLRPLRAEVKLSPPNPANMQSWVELAEKQSFPVVIQDSATEIASLQTKFARAGHY
                     PTLDIVGTYGQINQTGTTISALSSQANIGTIGLQLGMPLYQGGAISSREREAAANYEK
                     AKQDLENARRSSALSAQQNYLLVINGIATIGALEQALVSSQSALDSNKLGYEVGVRIN
                     IDVLNAQQQVFSTRRDLLQARYGTIMNHFRLKAAAASLREDDLEQANAALEP"
     gene            complement(1472..1795)
                     /locus_tag="VI195_11555"
     CDS             complement(1472..1795)
                     /locus_tag="VI195_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6825070.1"
                     /translation="MKSLAPAALAAWLKDPARKRPLLLDVREPWEFQTVRLENSMLVP
                     MREIPARLGELDATAEIVVICHHGGRSLQAALFLDRQGFASVHNLAGGVDAWARQVDP
                     AMPVY"
     gene            complement(1803..2480)
                     /locus_tag="VI195_11560"
     CDS             complement(1803..2480)
                     /locus_tag="VI195_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY6825071.1"
                     /translation="MNLEQARSNMVEQQIRPWDVLDQDVLDLLYTVPREEFVPPAYRN
                     LAFTDMEIPLRKNAAEGERMWAPKIEARVLQAVAPRRSDRVLEVGTGSGYLTALFAHR
                     AAQVCSVEINPELAAFGKANIARHGVDNVQLQVADGARGVAPGFPFEGPFDVILLTGS
                     VPLVPRALLEALAPGGRAFAVVGELPVMTAKIFHCGAPGEYRAVELFETVLAPLAHCE
                     RPSRFRF"
     gene            complement(2509..>4426)
                     /locus_tag="VI195_11565"
     CDS             complement(2509..>4426)
                     /locus_tag="VI195_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6825072.1"
                     /translation="VRENIVRHVALGADHRQAALEGTREIGLAVLATTFSIVAVFLPV
                     GFMGGIIGRFFHQFGLTVVSAVLISMFVSFTLDPMLSSIWHDPQAEGKFSTGPMGRLL
                     AWFHAFMERVSVGYERLLGWALAHRKAVLAIAFASFFASFPLLKLVGSEFVPEPDLSE
                     LQVQFKTPVGASLELTTLKARQAEAALREFPEVKYTYATVNTGFVQGKNKVNIFVQLV
                     PRKARQRSQNDLTKPMRERLGRIAGIELQQIGAYKTVSSGKPLQVSILGQERKVLAEI
                     SQQVLAITREVPGAVDVESSDEAAKPQVAVELKRELASDLGIGVGQVAATLRPLLAGE
                     AVGNWKGPDDQYYDVQVRLSRADRARAEDLQRVPLASTQTDANGAPRMVSLREIASVA
                     PGSGPQQINRKEQLREVLITANVFNRPAGDAATELRAKLDRIALPPGYRFTMGGSSKD
                     IQESLYFASQALALAVIFIYLILASQFGSFIQPVAIMMSLPLSLIGVVLALIFARSTM
                     NIFSIIGFIMLMGLVTKNAILLVDFVNQERGQGKPRRDAVLAAGRIRLRPILMTTLAM
                     IFGMIPLALGLGEGGEQRAPMGQAVIGGVITSGLLTLVVVPVIYTYLDDLAVWVKRFG
                     KMAATRDNPAGTRT"
BASE COUNT          656 a         1485 c         1589 g          696 t
ORIGIN      
        1 agcttctatc gaaccccggg gaaagaagtc gaatgggcgc ggcgggaagg gcgctttgcc
       61 aggcgcatcg cggcgcgacg cgcaggcacg tcgagtactg cggggaactg ctgcgcagcg
      121 gggctaaggc tccagcgcgg cgttagcctg ctcgaggtcg tcctcgcgca ggctcgcggc
      181 ggcggccttc agccggaagt ggttcatgat cgtgccgtag cgcgcctgca gcaggtcgcg
      241 ccgcgtggag aacacctgct gctgcgcgtt cagcacgtcg atgttgatgc gcacgccgac
      301 ttcgtagccg agcttgttcg agtcgagcgc gctctgcgag gacaccagcg cctgctcgag
      361 cgcgccgatg gtggcgatgc cgttgatgac gagcaggtaa ttctgctgcg ccgagagcgc
      421 gctcgagcgg cgcgcgttct cgaggtcctg cttggccttc tcgtagttgg ccgcggcttc
      481 gcgctcgcgc gagctgatgg cgccgccctg gtacagcggc atgccgagct gcaggccgat
      541 ggtgccgatg ttcgcctgcg agctgagcgc gctgatcgtg gtcccggtct ggttgatctg
      601 cccgtaggtg ccgacgatgt cgagcgtggg gtagtggccg gcgcgcgcga acttggtctg
      661 caggctggcg atctcggtgg ccgagtcctg gatcaccacc gggaaggact gcttttcggc
      721 gagctcgacc cagctctgca tgttggccgg gttcggcggc gagagcttca cctccgcgcg
      781 cagcggccgc agccgcgtgt agaccttgcc ggtcagctgc tgcagcgcgc ggatcttcgt
      841 gtcgacgtcg ttctgcgcga cgatgccctg cgcggtcgtc aggtcgtagc gcgcctgcgc
      901 ctcgtgcgtg tcggtgatgg tggcggtgcc gacctcgaag ttgcgcttgg cttgcgcgag
      961 ctgctcgccg atggcgacct tctgcgcgtt gaccagcgtg aggttgtcct gcgccgcgag
     1021 cacgtccacg taggcctgcg tgacgcgcag gatcaggtcc tggctcgcct ggccgaacgt
     1081 ggcctcggac tgccgcacct gctgctcggc ctggtcgtac tgcgcccagt tctgcgcgcg
     1141 gaacaccggc tgcgccaggg gcagctggta gttgcggttg tagaccgggc gcgtgcccgc
     1201 gggcaccggg gtcggcccgt aaggactgag ctcgaggtgg ttggtagtgg tggccgcggt
     1261 caggccgagc gtgggcagca gcagcgcgcg cccctgcggc agcttctcca ggcccgcttg
     1321 cagcgcctgc ttggccgagg cgtactgcgc atcgtaggcc ttggcgtcgc gatagacctg
     1381 cagcaggtct tcggccgccg ccggcgcggc ggcgaatgcg agggacagca ggaacaaccg
     1441 gactcggatg cggcgcatgc ggcggtcccc gtcagtagac cggcatcgcc ggatcgacct
     1501 ggcgcgccca ggcatccacg ccgcccgcca ggttgtgcac gctcgcgaaa ccctgccggt
     1561 ccaggaacag cgccgcctgc aggctgcggc cgccgtggtg gcagatcacc acgatctcgg
     1621 ccgtggcgtc gagctcgccg aggcgcgccg ggatctcgcg catcggcacg agcatggaat
     1681 tctccagccg caccgtctga aactcccagg gctcgcgcac gtcgagcagc agcggtcgct
     1741 tgcgcgccgg gtctttcagc catgcggcca gcgcggccgg cgcgaggctc ttcaccggca
     1801 tctcagaacc tgaagcgcga gggccgctcg caatgggcga gcggcgcaag caccgtctcg
     1861 aacagctcga ccgcgcggta ttcgccgggc gcgccgcaat ggaagatctt ggcggtcatc
     1921 accggcaact cgccgaccac cgcgaacgcg cgcccgcccg gcgcgagcgc ctcgagcagc
     1981 gcgcggggta cgaggggcac cgagccggtg agcaggatca cgtcgaacgg accctcgaac
     2041 ggaaagcccg gcgcgacgcc gcgtgcgccg tcggcgacct gcagctgcac gttgtccacg
     2101 ccgtggcgcg cgatgttcgc cttgccgaag gccgccagct ccggattgat ctcgaccgag
     2161 cagacctgcg cggcgcggtg cgcgaacagc gcggtcaggt agccgctgcc ggtgccgacc
     2221 tcgagcacgc ggtcgctgcg ccgcggcgcg acggcctgca gcacgcgcgc ctcgatcttc
     2281 ggcgcccaca tgcgctcgcc ctcggccgcg ttcttgcgca gcgggatttc catgtcggtg
     2341 aaagccaggt tgcggtaggc cggcggcacg aattcctcgc gcggcaccgt gtagagcagg
     2401 tcgagcacgt cctggtccag cacatcccag gggcggatct gctgctcgac catgttgctg
     2461 cgggcttgct cgaggttcat cggggccgga tcgggatcgg acgcggagct aagtgcgggt
     2521 accggcggga ttatcccgcg ttgccgccat cttaccaaag cgtttcaccc agacggcaag
     2581 atcgtcgagg taggtgtaga tcaccggcac caccaccagc gtgagcaggc ccgaagtgat
     2641 cacgccgccg atcaccgcct ggcccatcgg cgcgcgctgc tcgccgccct cgccgaggcc
     2701 cagcgcgagc gggatcatgc cgaagatcat ggccagcgtc gtcatgagaa tcggtcgcag
     2761 gcggatgcgg cccgcggcga gcacggcgtc gcgccgcggc ttgccctggc cgcgctcctg
     2821 gttgacgaag tcgaccagca ggatcgcgtt cttggtgacc aggcccatga gcatgatgaa
     2881 gccgatgatc gagaagatgt tcatcgtcga gcgcgcgaag atcagcgcca gcaccacgcc
     2941 gatcagcgac agcggcagcg acatcatgat cgccaccggc tggatgaagc tgccgaattg
     3001 cgaggcgagg atcaggtaga tgaagatcac cgccagcgcg agcgcctggc tggcgaagta
     3061 aagcgactcc tggatgtcct tggaggagcc gcccatggtg aagcggtagc ccggcggcag
     3121 cgcgatgcgg tccagcttgg cgcgcagctc ggtggccgcg tcgcccgcgg gccggttgaa
     3181 cacgttggcg gtgatgagca cttcgcgcag ctgctccttg cggttgatct gctgcggccc
     3241 gctgcccggc gcgaccgacg cgatctcgcg caggctgacc atgcgcggcg cgccgttggc
     3301 gtcggtctgc gtggaagcga gcggcacgcg ctgcaggtcc tcggcgcgcg cgcggtcggc
     3361 gcgcgacagc cgcacctgca cgtcgtagta ctggtcgtcg ggccccttcc agttgccgac
     3421 cgcctcgccg gcgagcagcg ggcgcagcgt ggcggcgacc tggccgacgc cgatgccgag
     3481 atcgctggcg agctcccgct tcagctccac ggcgacctgc ggcttggccg cctcgtcgct
     3541 cgactccacg tccaccgcgc cggggacctc gcgcgtgatc gccagcacct gctgggagat
     3601 ctcggcgagc accttgcgct cctggccgag gatcgagacc tgcagcggct tgccgctgga
     3661 gacggtcttg taggcgccga tctgctgcag ctcgatgccg gcgatgcggc ccaggcgctc
     3721 gcgcatcggc ttggtgaggt cgttctgcga gcgctggcgc gccttgcgcg gcacgagctg
     3781 cacgaagatg ttgaccttgt tcttgccctg cacgaagccg gtgttgacgg tggcgtaggt
     3841 gtacttcacc tcggggaatt cgcgcagcgc ggcttcggcc tggcgcgcct tgagcgtggt
     3901 gagctcgagg gaggcgccca ccggcgtctt gaactgcacc tgcagttccg acaggtccgg
     3961 ctcgggcacg aactcgctgc cgacgagctt cagcagcgga aagctcgcga agaagctcgc
     4021 gaaggcgatc gccagcaccg ctttgcggtg cgcgagcgcc cagccgagca gccgctcgta
     4081 gccgacggac acgcgctcca tgaaggcgtg gaaccaggcc agcagccggc ccatgggtcc
     4141 cgtgctgaac ttgccctcgg cctgcggatc gtgccagatc gaggacagca tggggtcgag
     4201 cgtgaacgac acgaacatcg agatcagcac cgccgaaacc acggtcaggc cgaactggtg
     4261 gaagaagcgc ccgatgatgc cgcccatgaa gcccaccggc aggaacaccg cgacgatgga
     4321 gaaggtggtc gccagcaccg ccaggccgat ctcgcgggtg ccctcgagcg ccgcctggcg
     4381 gtggtcggcc ccgagcgcca cgtggcgcac gatgttctcg cggacc
//
LOCUS       DATDMP010000659         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2215895, whole genome shotgun sequence.
ACCESSION   DATDMP010000659 DATDMP010000000
VERSION     DATDMP010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215895"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2303)
                     /locus_tag="VI195_11570"
     CDS             complement(<1..2303)
                     /locus_tag="VI195_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6825073.1"
                     /translation="MPRLPEEVRSLGVNTVKASSNLTLVVNLTSPNGRYDSLYLRNYA
                     VLNIRDVLARLPGMGDVQVFGGGDYSMRVWLDPQKLAARNITAGDVVAAIREQNVQVA
                     AGQLGAPPSNTEFQIALNAAGRLTDEQQFRDIIIKTGDDGQVVRLGDIARVELGAQAY
                     GVRSLLDNKPSLAIPVFQSPGANALELSNSVRSTMEELKKNFPEGMDYGILYDPTQFV
                     RQSIDAVLHTLLEAVALVVLVVILFLQTWRASLIPLIAVPVSVIGTFAVLLAFGFSIN
                     TLSLFGLVLAIGIVVDDAIVVVENVERHIAAGLSPREATKVAMGEVSRPIIAITLVLC
                     AVFVPVAFVSGLTGQFYRQFALTIAISTVISAFNSLTLSPALAALLLKPHGAAPDALS
                     RLMNRLLGGFFQRFNRGFARGGAQYSRALGLTMRHGTIALAIYAGLIVLTWFGFNTVP
                     AGFIPAQDKQYLVAVAQLPPAASLDRTEAVTRRIADIALRQPGIAHAVEFAGMSVNGF
                     TQSSSAALIFFPLDDFSARSARALSGPAIAAALNAQMASIQDAFVMVVTPPPVIGLGT
                     LGGFKLQVEDRGAAGPEALFSALSDALGKANQNPALGGAFSTYQINVPQLDVAVDRVK
                     VKRENVKLSDVFETLQVYLGSLYVNDFNRFGRTYQVVAQADAPFRSQLDDILPLKTRN
                     ASGEMVPLGSVLNVSQSFGPDVVQRYNAYASADINGGPAPGFSSGQAQAAMAKILDAT
                     LPRGMAYEWTDLAYQQLISGNTAVLVFP"
BASE COUNT          439 a          733 c          799 g          352 t
ORIGIN      
        1 ggaaacacca gcaccgccgt gttaccggag atcagctgct ggtaggcgag atcggtccac
       61 tcgtacgcca tgccgcgcgg cagtgtcgca tcgagaatct tcgccatcgc cgcctgcgcc
      121 tgaccggagc tgaacccggg cgccggccca ccgttgatat ccgcggaggc ataggcgttg
      181 taacgctgta ccacatccgg tccgaaggac tgtgagacgt tgagcaccga gccgagcggc
      241 accatctcgc cgctggcgtt gcgggtcttc agcggcagaa tgtcatcgag ctgcgagcgg
      301 aacggtgcgt ccgcctgcgc caccacctga taggtgcgcc cgaagcggtt gaagtcattg
      361 acatacaagg agccgagata gacctgcagc gtctcgaaca cgtccgagag cttcacgttc
      421 tcgcgcttga ccttgacgcg atccacagcc acgtcgagct gcggcacgtt gatctgatag
      481 gtgctgaagg caccgccgag cgcggggttc tgattcgcct tgccgagcgc atccgaaagc
      541 gcgctgaaga gcgcctcggg tccggccgcg ccgcgatcct cgacctgcag cttgaagcca
      601 ccgagggtac ccagaccgat caccggtggc ggcgtcacca ccatgacgaa ggcatcctgg
      661 atggaagcca tctgcgcatt gagcgctgcg gcgatcgccg gtcccgagag cgcgcgcgcg
      721 gagcgcgccg agaagtcatc gagcgggaag aagatcagcg cggcactcga gctctgcgta
      781 aagccattca ccgacatgcc ggcgaactcg accgcgtgag cgatccccgg ctgcctgagg
      841 gcgatgtcgg cgatgcggcg cgtgaccgct tcggtacggt cgagcgaggc ggccggcggc
      901 agctgcgcga ccgccaccag gtactgcttg tcctgcgcgg ggatgaaccc ggcgggcacc
      961 gtgttgaagc cgaaccaggt gagcacgatg aggccggcgt agatggcgag cgcgattgtg
     1021 ccgtggcgca tggtgaggcc gagcgcgcgc gagtattgag cgccgccgcg ggcgaagccg
     1081 cgattgaagc gctggaagaa gccaccgagg aggcggttca tcaggcgcga cagcgcatcg
     1141 ggcgcggcgc catgcggctt gagcaacagc gccgcgagcg ccggcgacag ggtgagtgag
     1201 ttgaaggccg agatcaccgt ggagatcgcg atggtgagcg cgaactggcg atagaactgc
     1261 ccggtgagcc cgctcacgaa cgcgaccggg acgaacacgg cgcacaacac cagtgtgatc
     1321 gcaatgatcg gccggctcac ttctcccatc gccaccttgg tggcctcgcg cggcgagagc
     1381 cccgcggcga tgtggcgctc gacgttctcg accacgacga tggcatcatc cacgacgatg
     1441 ccgatcgcga gcaccaggcc gaacagcgac agcgtgttga tcgagaaacc gaaggcgagc
     1501 agcaccgcga aggtgccgat caccgagacc ggcacggcga tcagcggaat gagcgaggcg
     1561 cgccaggtct gcaggaacag gatgaccacc agtacgacca gcgccacggc ttcgagcagc
     1621 gtgtgcagca cggcgtcgat ggactggcgc acgaactgag tcgggtcgta gagaatcccg
     1681 tagtccatgc cctcggggaa attcttcttc agctcctcca tcgtgctgcg cacgctgttg
     1741 gaaagctcga gcgcgttggc gcccggtgac tggaataccg gtatggcgag tgagggcttg
     1801 ttgtcgagca ggctgcgcac gccgtaggcc tgggcaccga gctcgacgcg cgcgatatcg
     1861 ccgaggcgca ccacctggcc gtcatcgccg gtcttgatga tgatgtcgcg gaactgctgc
     1921 tcgtcggtaa gccgtcccgc ggcattcagc gcgatctgaa actcggtgtt ggaaggcggc
     1981 gcaccgagct gcccggcggc gacctgcacg ttctgctcgc ggatcgccgc caccacgtcg
     2041 ccggcggtga tattgcgcgc ggcgagcttc tgcggatcga gccagacgcg catggaatag
     2101 tcgccgccgc caaacacctg cacatcgccc atgcccggca ggcgcgcgag cacgtcgcgg
     2161 atattcagca ccgcgtagtt acgcaggtag agcgagtcat aacgcccgtt gggagaggtc
     2221 aggttcacga ccagcgtcag attggaggag gccttgacgg tattgacacc gaggctgcgc
     2281 acctcctcgg ggagccgtgg caaggcctgc gcgacgcggt tct
//
LOCUS       DATDMP010000660         3618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_215143, whole genome shotgun sequence.
ACCESSION   DATDMP010000660 DATDMP010000000
VERSION     DATDMP010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215143"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2606)
                     /locus_tag="VI195_11575"
     CDS             complement(<1..2606)
                     /locus_tag="VI195_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6825074.1"
                     /translation="MLDLPPLIDRRECMKLMGAALALAGSSCARPPLEQMVPYISTPE
                     GMVPGEPLFFATALTRGGYAHGVLIESNTGRPTKIEGNPAHPASLGATGAIEQGRILE
                     LWDPERSRAALHLGQISSWDSFDDALLDPAAPWRRSAGDGLRILSGHVSSPTLLAQRQ
                     RLLERYPRARWHEHEAVGRASVYEGTRFAFGRPLEPLFHFDRAAIVVSLEADFLGEEP
                     GQVRYAREFAAGRRPERGTMSRLYVAECMPSLTGVAADARMPCRPSEVVTIAQQIAAH
                     LDGRAEDQVNPWLRAVLADLHTHRGRSLVLAGAGQPARVHALTHYINGALGNLGSTVE
                     FIAPGGAVPAQEESIGALARDMRLGRVDTLLILDGNPVYDAPVDLEFASALERVRVSV
                     HHGLYVDETAEACGWHAPAAHELESWSDTRAFDGTITLQQPLIAPLHGGRSAHELLGL
                     LTEERSMSAHDAVRATWLQRFGAADFAERWRAALRLGVIEGTAAQPEPVGKPALAPRA
                     DVEPAGPLELLFRPDPTLWDGRYAPIAWLQELPKPLTQLTWDNAALMSPQLATQLGVR
                     NEELIELSIGGRSLAAPVWVVPGHAAGAVTLHLGYGRTRAGPVGSNRGSNAFALRTAD
                     APWYAHGLSVRKLSGRRALATVQHHHRMEGREIVRSATLPTFRERPDFAQHASERQQP
                     SIYPPFPAPGYSWGMSIDLSACIGCGACTIACQAENNIPTVGKEEVLRGREMHWIRID
                     RYTEGDGAATRDHFQPVPCMQCEHAPCELVCPVEASVHDTEGINVQVYNRCVGTRFCS
                     NNCPYKVRRFNFLQYSRDEPHLNAQRNPQVTVRMRGVMEKCTYCLQRITTAKIAADGE
                     NRRLRDGEVVTA"
     gene            complement(2585..3274)
                     /locus_tag="VI195_11580"
     CDS             complement(2585..3274)
                     /locus_tag="VI195_11580"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY6825075.1"
                     /translation="MAPALHAARGRANVQTFSPGEVLWLKLGFAGLVVAVCAALFGWR
                     TLIAPRPAVGAPVEQSVPFSHRHHAGDDGIDCRYCHSSVETEAFAGLPALSTCMTCHS
                     QLFTNAPLLQPLRRSFAGVGTLRWNRVHHLPDFAYFDHSIHVSKGIGCSTCHGQVDAM
                     PLTYRVASLRMEWCLDCHRAPERYLRPSTAVFDMHWRAPPDQLERGRRLRASYQLHPT
                     EVLTACSTCHR"
BASE COUNT          601 a         1194 c         1257 g          566 t
ORIGIN      
        1 caggccgtga cgacttcgcc gtcgcgcagg cgccggttct cgccgtcggc ggcgatcttc
       61 gccgtggtaa tgcgctgcag gcagtaggtg cacttctcca tgacgccgcg catgcgcacc
      121 gtgacctgcg ggttgcgctg cgcgttgaga tgcggttcat cgcgcgagta ctgaaggaaa
      181 ttgaagcgcc gcaccttgta gggacagttg ttggagcaga agcgcgtgcc gacgcagcgg
      241 ttgtagacct gcacgttgat gccctcggtg tcgtgcaccg acgcctcgac cgggcagacg
      301 agctcgcagg gcgcatgctc gcactgcatg cagggcaccg gctggaaatg atcgcgcgtg
      361 gccgcgccat caccctcggt gtagcgatcg atgcggatcc agtgcatttc cctgccgcgc
      421 agcacttcct ccttaccgac cgtgggaata ttgttttccg cctgacaggc tatggtgcag
      481 gccccacagc cgatgcaggc gctcagatcg atactcatgc cccaggagta gcccggcgca
      541 gggaacggcg ggtagatcga tggctgctgc cgctcactcg cgtgctgcgc gaagtcgggg
      601 cgctcgcgaa aggtcgggag cgtcgcgctg cgtacgatct cgcgaccctc catgcggtgg
      661 tggtgctgca cggtggcgag tgcgcgtctg ccggaaagct tgcgcacgct gaggccgtgc
      721 gcgtaccagg gggcgtccgc agtgcgcagc gcaaatgcgt tgctgccgcg attcgagccg
      781 accggcccgg cgcgcgtgcg cccgtagccg aggtgcagcg tcacggcgcc ggccgcgtgc
      841 ccgggaacga cccacaccgg cgccgcgagg cttcggccac cgatgctcag ctcgatgagc
      901 tcctcattgc gcaccccgag ttgtgtggcg agctgcgggc tcatgagcgc ggcgttgtcc
      961 caggtgagct gcgtgagcgg cttcggcagc tcctgcagcc aggcgatggg cgcgtagcgc
     1021 ccgtcccaga gcgtcggatc cgggcgaaac agcagctcga gcgggcccgc gggctcgaca
     1081 tcggcacgcg gcgcaagtgc cggcttgccc acgggctcgg gctgcgcggc agttccttcg
     1141 atgacgccga gccgcagcgc ggcacgccaa cgctcggcaa agtccgccgc accgaagcgt
     1201 tgcagccagg tggcgcgcac ggcatcgtga gcgctcatag agcgctcctc ggtcagcaac
     1261 cccagcaact cgtgtgccga gcggccgccg tgcagcggcg cgatcagcgg ctgctgcagc
     1321 gtgatcgtcc cgtcaaaggc gcgtgtgtcg ctccacgatt cgagctcatg tgccgcaggc
     1381 gcatgccagc cgcatgcctc cgcggtctca tcgacgtaaa gtccatggtg cacactcacg
     1441 cgcacccgct cgagtgcgga ggcaaactcg aggtccacgg gagcgtcgta caccggatta
     1501 ccgtccagga tcagcaaggt atcgacgcgc ccgaggcgca tatcgcgcgc caacgcgccg
     1561 atcgattctt cctgcgcggg caccgcgccg cccggtgcaa tgaattccac cgtagacccg
     1621 agattgccga gcgcgccgtt gatgtagtgg gtgagtgcgt gcacgcgagc gggctgtccc
     1681 gccccggcga gaaccagcga gcggccacga tgggtgtgca ggtccgcaag cactgcgcgc
     1741 agccacggat taacctgatc ttctgcgcgc ccatcgaggt gtgcggcgat ttgctgcgcg
     1801 atcgtcacca cctcgctcgg acggcacggc atgcgcgcat ccgccgcaac gcccgtgagc
     1861 gagggcatgc actccgcgac gtacagacgg ctcatcgtgc cgcgctcggg acggcgcccg
     1921 gcggcgaact cgcgtgcgta acggacctga cccggctcct caccgagaaa atcggcctcc
     1981 aatgagacca cgatggcggc acgatcgaag tggaacagcg gctcgagcgg gcgaccgaag
     2041 gcgaaccggg tcccctcgta cacactcgcg cgcccgaccg cctcgtgctc atgccagcgg
     2101 gcgcgcggat agcgttcgag cagccgctgc cgctgcgcga gcagagtggg ggagctcacg
     2161 tgcccgctga ggatgcgcag gccatcaccc gcgctgcgac gccagggggc ggccgggtcg
     2221 agaagcgcgt catcaaatga gtcccaggaa gaaatctgtc ccaggtggag cgctgcgcgc
     2281 gagcgttccg gatcccagag ctcgaggatt ctcccttgct cgatggcacc cgtcgccccg
     2341 agactcgccg ggtgcgcggg atttccttcg atctttgtcg gccgcccggt gttgctctcg
     2401 atgagcaccc cgtgggcata gccgccgcgc gtgagggccg tggcgaagaa cagcggctcg
     2461 ccgggcacca tcccctcagg cgtgctgatg taaggcacca tctgctcgag cggagggcgt
     2521 gcgcagctgc tgccggcgag cgcgagcgct gcgcccatga gtttcatgca ctcacggcga
     2581 tcgatcaacg gtggcaggtc gagcatgccg tgagcacctc ggtggggtgg agttggtatg
     2641 aggcgcgcag ccgccggccg cgctcgagct ggtcgggcgg cgcgcgccag tgcatgtcga
     2701 aaaccgccgt ggacggtcgc agatagcgct cgggcgcgcg gtggcagtcg agacaccact
     2761 ccatccgcag cgacgccacc cggtaggtca gcggcatggc atcgacctgc ccgtgacagg
     2821 tgctgcagcc gatgcccttg ctcacgtgga tcgagtgatc gaaataggcg aaatccggca
     2881 ggtggtggac gcggttccag cgcagcgtgc cgacccccgc gaaactcctg cgcagtggct
     2941 gaagcagcgg tgcgttggtg aacagctgcg agtgacaggt catgcaagtg ctgagcgccg
     3001 gcagaccggc gaacgcttcg gtctccacgc tgctgtgaca gtagcgacag tcgatgccgt
     3061 catcgccggc gtgatgtcgg tgactgaacg gtacgctctg ctcaaccggc gccccgacag
     3121 ccgggcgcgg cgcgatgagc gtgcgccagc cgaagagcgc cgcgcacact gcaacaacca
     3181 gcccggcgaa gccgagcttc agccacagga cttcgccggg cgaaaaggtc tgcacgttag
     3241 cgcggccgcg cgcggcatgg agcgccggcg ccactcacca catcgcgtcg acgaaaagtc
     3301 atgttggttt tcggaccgct gcttgctcga acatgttcca acactgcatc ggaagcaagc
     3361 gagtgccgaa gcgccgcagc aagctgagca ggagttgcca acatcaccaa catgacaggt
     3421 gccgtcgcgg ctgagcgccg agcattgggc tgttatcttt gtcggaatgt gccgatgcgg
     3481 ttcgaggagg tgctcggtgg cgggttagct gccctctgag ccaggggcgc gcagcttgtg
     3541 gacgtcgccc gcgctgagcg caggcgccgc atgtccccag ctgttgcgca gataggtggt
     3601 gacggccgca atctgcgc
//
LOCUS       DATDMP010000661         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1437803, whole genome shotgun sequence.
ACCESSION   DATDMP010000661 DATDMP010000000
VERSION     DATDMP010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437803"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..571)
                     /gene="bamE"
                     /locus_tag="VI195_11585"
     CDS             complement(167..571)
                     /gene="bamE"
                     /locus_tag="VI195_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY6825076.1"
                     /translation="MQLVIILHRIRMRLILASLATVLLSAALGGCVYRMDVQQGNYLE
                     GKTVDQLQVGMTRTQVRYLLGTPLVPDVFDKDRWDYLYYFRHGRRRPDAPRRLEVYFK
                     EDKVTHFERINVPESAPQAPDEGAPISKFPKI"
     gene            627..1058
                     /gene="fur"
                     /locus_tag="VI195_11590"
     CDS             627..1058
                     /gene="fur"
                     /locus_tag="VI195_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY6825077.1"
                     /translation="MEGKDLRKAGLKVTLPRLKILEILEGSTTRHLSAEDIYRTLLES
                     NEDIGLATVYRVLTQFEAAGLVTRHHFEDGMAVFELNQGTHHDHIVCLDCGRVEEFVD
                     SGIEERQTAVASKLGFTIRDHSLILYGHCRREHCPYRPRKS"
     gene            complement(1081..>2459)
                     /locus_tag="VI195_11595"
     CDS             complement(1081..>2459)
                     /locus_tag="VI195_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825078.1"
                     /translation="GAAQRELLDGYGSLTASVGQVGIAHRVWLELLNRTLALEGQARD
                     RDSKLELLRYQVQELRALQLKPGEAEALAEEAARLANRGRLAGGAQASLDALYANEQL
                     SAHAMVSRALQSLRPLAALDGKLAAVLPLLEEAAVQVREAARELERYGESLELDSARQ
                     EEVQRRLAAVEELARKHHAAPAELPGYGQRLGAELEALERADTDLADLRRELATALQA
                     YRAQAQQLSGKRMATARVLARDISARMQTLGMAGGRFEADVSQDGNADPSQHGIDQVE
                     FRVTANPGSPPRTLAKVASGGELARLSLAVQVACASQDSRCMVFDEVDAGIGGAVAEI
                     VGRELRALGVRSQVLCVTHLPQVACQAHHHLRVTKATDGRTTRTTLAELDGDERIEEL
                     ARMLGGVEITSKAREHARDMLSAATLAGGKTAAAGVAGTKAEVHGESAGKSGDTVRQP
                     RPPAARRR"
BASE COUNT          372 a          844 c          852 g          391 t
ORIGIN      
        1 cgcagatccc ctgggactcc gcaccggtcg gtatcttcgg ggagccgcgc agccgcggcg
       61 atcgctgcgc ggagggcgat cgcatcgaga tctaccgtcc gctcaagcgc gatccgcgcg
      121 agcggcgccg cgagctcgtg caacgcgaac gccgcggcgg tcgccgctag atcttcggga
      181 atttcgagat gggcgcgccc tcatcgggcg cctgcggcgc gctctcgggc acgttgatgc
      241 gctcgaagtg cgtcactttg tcctccttga aatacacctc gaggcgccgc ggcgcatccg
      301 gacggcggcg gccgtggcgg aagtaataaa ggtaatccca gcggtccttg tcgaacacgt
      361 caggcaccag cggtgtgccg aggaggtagc gcacctgagt gcgcgtcatg ccgacctgga
      421 gctgatcgac ggtcttaccc tcgaggtagt tgccctgctg cacatccatc cggtagacgc
      481 agccgccgag cgctgcgctg agcagtaccg tcgcgagtga ggcgagaatc aagcgcatgc
      541 gaatccgatg gaggataatc acgagctgca tgatacctga tcgcggagcg gcccctggcc
      601 ccttcaggcg ctccgccgga ggcgacgtgg aaggcaagga cctgcgcaag gcgggtttga
      661 aagtgacgct gccgcggctg aagatcctcg agatcctcga gggcagcacc acgcggcacc
      721 tgtcggccga ggacatctat cggacgctcc tcgagtcgaa cgaggacatc ggacttgcga
      781 ccgtataccg ggtgctcacg cagttcgagg cagctgggct ggtcacgcgc catcacttcg
      841 aggacggcat ggcggtcttc gagctcaacc agggcacgca ccacgatcac atcgtgtgtc
      901 tcgactgcgg ccgggtggag gagttcgtgg actccggcat cgaggagcgt cagaccgccg
      961 tggccagcaa gctcggcttc accatccgcg atcattcgct gattctctac gggcattgcc
     1021 ggcgcgagca ctgtccctac cgcccgcgca agtcctagcg aggagatttt tcaggcgggc
     1081 ctagcggcgg cgtgcggccg gcggccgcgg ctggcgtacc gtgtccccgg acttgccggc
     1141 agactccccg tgtacctccg ccttggttcc agccactccc gcggcggcgg tcttgccgcc
     1201 cgcgagcgtc gcagccgaca gcatgtcgcg tgcgtgctca cgcgcctttg atgtgatctc
     1261 gacgccaccg agcatgcggg cgagctcctc gatgcgctca tcgccgtcga gctcggcgag
     1321 cgtggtgcgc gtcgtgcggc cgtcggtggc cttggtaacg cgcagatgat gatgcgcctg
     1381 gcaggcgacc tgcgggaggt gcgtgacgca taaaacctga ctgcgcacgc cgagcgcgcg
     1441 cagctcgcgg ccgacgatct ccgccacggc gccgccgatg ccggcgtcga cttcatcgaa
     1501 caccatgcag cggctgtcct gcgaggcgca ggcgacctgc acggcgagcg agagccgcgc
     1561 cagctctccg cccgaggcga ccttggcgag cgtgcgcggc ggtgagccgg gattagcggt
     1621 gacgcggaac tcgacctggt cgatgccgtg ctgggaggga tcggcattgc catcctggga
     1681 tacatccgct tcgaagcgac cgccggccat gccgagggtc tgcatgcgcg cgctgatgtc
     1741 gcgcgcgagc acgcgcgcgg tcgccatgcg tttgcccgag agctgctgcg cctgggcgcg
     1801 gtaagcctgg agcgccgtgg cgagctcccg gcgcaagtcc gcgagatcgg tatccgcgcg
     1861 ctcgagcgcc tcgagctccg cgccgagacg ttgcccgtac cccggcagct ccgccggggc
     1921 agcgtgatgc ttgcgcgcga gctcctcgac cgccgctaga cgccgctgca cctcctcctg
     1981 gcgcgcgctg tcgagctcga gcgactcacc atagcgttcg agctcacgcg cggcttcgcg
     2041 cacctgcacc gcggcctctt ccagcagcgg cagcaccgca gcgagcttgc catcgagcgc
     2101 ggcgagcggg cgcagcgatt gcagggcgcg gctcaccatg gcgtgggcgc tcagctgctc
     2161 attcgcatag agcgcatcga gcgaggcctg tgcgccgccg gccaggcggc cgcggttggc
     2221 gagccgtgcg gcctcttcag cgagcgcttc cgcttcgccg ggcttgagct gcaacgcacg
     2281 cagctcctgc acctggtagc gcagcagctc gagtttcgag tcgcgatcgc gcgcctgtcc
     2341 ttcgagcgcc agcgtgcggt tgagcagctc gagccacacc cggtgtgcga tgccgacctg
     2401 gccgacgctc gcagtgagcg agccgtagcc atcgagcagc tcgcgttgcg ccgcgccgc
//
LOCUS       DATDMP010000662         3359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_659947, whole genome shotgun sequence.
ACCESSION   DATDMP010000662 DATDMP010000000
VERSION     DATDMP010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659947"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VI195_11600"
     CDS             complement(<1..307)
                     /locus_tag="VI195_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6825079.1"
                     /translation="MISQEQNDLMTRVGPGTPAGNLLRNYWQPVALVDQFLGLTRPVL
                     PIKVLGEEFVLFRDTKGKYGLLDRRCPHRQADLAYGRYEDGNLRCVFHGWLFDVNGKC
                     "
     gene            complement(380..1093)
                     /locus_tag="VI195_11605"
     CDS             complement(380..1093)
                     /locus_tag="VI195_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6825080.1"
                     /translation="MAEPMLAVDGLETAYGRSQVLFGVSLRIAPGEVATLLGRNGMGK
                     TTTVRSIMGMVRSGAGSIAFDGAYITGLPSYRIAQAGIGLVPEGRQIFPNLTVRENLV
                     ATAANRRNAKAPWTLERVFDLFGNLKNRSNNYGNQLSGGEQQMLAIGRALMTNPRLLI
                     LDEATEGLAPQIRQEIYRSIERLKSAGLSILVIDKDVKALIRVADVHYMLEKGRVVWT
                     GSSGELSGNQEVQQRYLGV"
     gene            complement(1093..1842)
                     /locus_tag="VI195_11610"
     CDS             complement(1093..1842)
                     /locus_tag="VI195_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6825081.1"
                     /translation="MSSALLETVGLRKSFGALVATDGVDFDVREGETHAVIGPNGAGK
                     TTFIKQLSGELLPDHGTIRFAGEDVTRLPGHLRARRGLARSFQITSIFHDFSTLDNVA
                     LAVQAHAGHSFHFWRAARGDASLTQPAMQLLKHVGLEYKAQVLATELSHGEHRQLEIA
                     MALATQPRLLLLDEPVAGMGTDESARMVKFLETLKGSKTIVLVEHDMDAVFSLADRIS
                     VLVYGRIVATGTPAQIRANAEVRAAYLGEES"
     gene            complement(1835..2797)
                     /locus_tag="VI195_11615"
     CDS             complement(1835..2797)
                     /locus_tag="VI195_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6825082.1"
                     /translation="MNASPRTWVLFGLLALLAAVPPLAAWLNEPFYLDLMRRVMIFAI
                     AAASLNLILGYGGMVSFGHAAYLGVGAYAVGVLGFYGIHDGYLQWGTAIGASALVALA
                     IGAVSVRTSGIYFIMITLAFTQMLYYLGVSLTEYGGDDGLRLSSRSQFPGVMDLADAT
                     TFYYLVLALLAGTLLLVHRLVNSRFGRAIQAARSNEARARAIGISPYPYRLAAFVIAG
                     AICGLAGALLVNQTSFLTPEFMNWTRSGELMFMVILGGAATSAGPLLGAAALLLLEDV
                     LQGWELLPREAVRDHWQLYLGVILVLVVLYAKRGLAGLIPGKHE"
     gene            complement(2794..>3359)
                     /locus_tag="VI195_11620"
     CDS             complement(2794..>3359)
                     /locus_tag="VI195_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6825083.1"
                     /translation="VYTSIPAWLQGHVDLFSGSRYPVYRLAVLAVGLAVAGLLWYVVT
                     RTRLGMLIRAGASNRTMVAALGINIRLLYTIVFGFGAALAGLAGLLAGPIYAVQPGMG
                     EQILIQVFVVIVIGGIGSIRGALAGALIVGMVDTLGRAFLKPTLATIFTQSAADAAGP
                     ALASMLIYLLMAVVLTFRPAGLFPAAR"
BASE COUNT          577 a         1109 c         1160 g          513 t
ORIGIN      
        1 ggcacttgcc gttcacgtcg aacagccagc cgtggaatac gcagcgcagg ttgccgtcct
       61 cgtagcgccc gtaggcgagg tctgcctggc ggtgcgggca gcgccgatcc agaagaccgt
      121 acttgccctt ggtgtcccgg aacagaacga actcctcgcc cagcaccttg atcggcaaaa
      181 caggtctggt caaacccaga aactgatcca cgagcgctac cggttgccag tagttgcgca
      241 gcagattgcc cgccggggtg cccggcccga cgcgagtcat gaggtcgttc tgttcctggc
      301 taatcatgcg attcgcagtc gcaagccttc agggccggcg tgtcctcgac gttcgcccgg
      361 ccaacgacgt tttcgcgctt cataccccga ggtacctttg ttgaacctcc tggttgccgg
      421 acagctcgcc cgagcttccg gtccagacga cgcgaccttt ttccagcatg tagtgtacgt
      481 ccgcgacgcg gatcagcgcc ttcacgtcct tgtcgatgac gaggatcgag aggcccgcgc
      541 tcttgaggcg ctcgatcgag cggtagattt cctggcggat ctgcggcgcg aggccctcgg
      601 tcgcttcatc caggatcagc aggcgcgggt tggtcatgag cgcgcggccg atcgccagca
      661 tctgctgctc gccgcccgaa agctggttgc cgtagttgtt cgacctgttt ttcagattgc
      721 cgaacaggtc gaacacccgc tcgagggtcc acggcgcctt cgcgttgcgc cggttggcgg
      781 cggtcgccac gaggttctcg cgcacggtca ggttcggaaa aatctgccgg ccctcgggca
      841 ccaggccgat gccggcctgc gcgatgcggt acgacggcag gccggtaatg taggcgccat
      901 cgaaggcgat gctgccggcc ccggagcgca ccattcccat gatcgaacgc acggtggtgg
      961 tcttgcccat gccgttgcgc ccgagcagcg tggcgacttc gcccggcgcg atgcgcagcg
     1021 acacgccgaa cagcacttgg ctccggccgt aagcggtctc caggccgtcc accgccagca
     1081 tcggctcggc catcagctct cctcgcccag gtaggcggcg cggacctccg cattggcgcg
     1141 gatttgcgcc ggcgtgccgg tggcgacgat gcgcccgtag accagcacgg aaatgcggtc
     1201 ggcgagcgag aacacggcat ccatgtcgtg ctcgacgagc acgatggtct tgctgccctt
     1261 gagcgtctcg agaaacttca ccatgcgcgc ggactcgtcg gttcccatgc cggccacggg
     1321 ctcgtccagc agcagcagtc gcggctgggt ggcgagcgcc atggcgattt ccagctggcg
     1381 gtgctcgccg tgggaaagct cggtggcgag gacttgcgcc ttgtattcga gacccacgtg
     1441 cttgaggagc tgcatcgcgg gttgggtcag cgaggcgtcg ccgcgcgccg cgcgccagaa
     1501 atggaagctg tggccggcat gcgcctgaac cgcgagcgcc acgttgtcca gcgtgctgaa
     1561 gtcatggaag atgctggtga tctggaagga gcgcgccagg cccctgcgcg cgcgcaggtg
     1621 tccgggcagt ctcgtgacgt cttcgcccgc gaaccggatc gtgccgtggt cgggaagcag
     1681 ctcgcccgag agctgcttga tgaaggtggt cttgccggcg ccgttcgggc cgatcactgc
     1741 gtgcgtctcg ccctcgcgca cgtcgaagtc cacgccgtcg gtggcgacca gcgcaccgaa
     1801 ggatttgcgc agccctacgg tctcgagcag cgcgctactc atgcttgccg gggatcagtc
     1861 ccgccagccc gcgcttggcg tacagcacca cgagcaccag gatcacgccg aggtacagct
     1921 gccagtggtc gcgcactgcc tccctgggca gcagttccca gccctgcagc acgtcttcca
     1981 gcagcagcag cgccgcagca ccgagcagcg gcccggcgct ggtggcggcg ccgccgagga
     2041 tcaccatgaa catgagctcg cccgagcgtg tccagttcat gaattcgggc gtcaggaacg
     2101 aggtctgatt gaccagcagc gcgccggcga ggccgcagat cgcgcctgcg atgacgaagg
     2161 ccgcgagacg gtaaggatag ggcgagatgc cgatggcgcg cgcgcgcgcc tcgttcgacc
     2221 gcgcagcctg gatcgcccgg ccgaaccgcg agttcaccag gcggtgcacc agcaggagcg
     2281 tgccggccag cagggcgagc accaggtagt agaacgtcgt agcgtcggca aggtccatca
     2341 cgcctgggaa ctggctgcgc gacgagagcc gcaggccgtc gtcgccgccg tactcggtga
     2401 gggacacgcc gaggtaatag agcatctgcg tgaaggcgag cgtgatcatg atgaagtaga
     2461 tgccgctggt gcgcaccgac accgcgccga tggcgagcgc gaccagcgcc gaggcgccga
     2521 tcgcggtgcc ccactgcaga tagccgtcgt ggatgccgta gaagccgagc acgcctaccg
     2581 cgtacgcgcc gacgcccagg tacgccgcgt ggccgaacga caccatgccg ccgtagccga
     2641 ggatcaggtt caggcttgcc gccgcgatcg cgaagatcat cacgcggcgc atcaggtcga
     2701 ggtagaaggg ctcgttcagc caggcggcga gcggcggcac ggccgcgagc agcgcgagca
     2761 ggccgaacag cacccaggtg cgcggcgagg cgttcatcgg gctgccggaa acaggcccgc
     2821 cggacggaat gtgagcacca ccgccatgag caggtagatg agcatcgagg cgagtgccgg
     2881 tcccgccgcg tcggcggccg attgcgtgaa aatcgtcgcg agcgtgggct tgaggaacgc
     2941 acgccccagc gtgtccacca tgccgacgat gagcgcgcct gcgagcgcgc cgcggatcga
     3001 gccgatgccg ccgatcacga tgacgacgaa cacctggatc aggatctgct cgcccattcc
     3061 cggctgcacg gcgtagatgg ggcccgcgag cagccctgcg aggcccgcga gcgccgcgcc
     3121 gaagccgaac acgatcgtgt aaagcaggcg aatgttgatg ccgagcgcgg cgaccatggt
     3181 gcggttcgac gcgcccgctc ggatgagcat gcccaggcgc gtgcgcgtca ccacatacca
     3241 gaggagcccc gcgaccgcga gcccgacggc gagcacggcc aggcggtaga ccgggtagcg
     3301 ggagccggag aagaggtcga cgtggccctg cagccatgcc gggatcgagg tgtagacgc
//
LOCUS       DATDMP010000663         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2105104, whole genome shotgun sequence.
ACCESSION   DATDMP010000663 DATDMP010000000
VERSION     DATDMP010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105104"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..746
                     /locus_tag="VI195_11625"
     CDS             27..746
                     /locus_tag="VI195_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6825084.1"
                     /translation="MTERPHVLIVDDDREIRGLLAQYLEKHDFRTTSVADGKEMRRVL
                     ERSHVDLLVLDLMLPGEDGLSLCRELRNRSQLPIIMLTARGEDVDRIIGLELGADDYV
                     AKPFNPRELLGRIKAVLRRSAHAPRDPSPESVHGFSFGGWRLDTVTRTLTHSEGRELA
                     LSGAEYRLLAVLLGAGNRVLTRAQLTELLRGRDADPFDRSIDVRVSRLRQILGDDARA
                     PQIIKTVYGEGYVIGVNVATD"
     gene            763..2190
                     /locus_tag="VI195_11630"
     CDS             763..2190
                     /locus_tag="VI195_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY6825085.1"
                     /translation="MRLSLWPQSLFGRLLAASVAAVLIAQAVALLLIAQEREHFMLQG
                     SVREWTRRIADTTLMLAPLNGPQRAELLAQLSATQALHAPHILLHRPGEPGGGAHELG
                     RGFLRLPLLRDFENALREQLRAALGPSYGIAIAPTPEPAPSAVVLPAPFYEAHELALH
                     PTSAQRYDVTVRFADGDAVTYRVMRMPGGAPLPRNLFLNLTLLVLLLVIVLYLAARNI
                     TRPLSDLARAADSVGRDSRPAPQLAERGARELRDAARAFNTMQDRLHRYLDSRTRVLA
                     AMSHDLKTPLTRLRLQVEALDNPPMQARIGRELDEMESMVREALSLFRGLDDGEPPTE
                     FDVDELLAQIRGEFADMGAAVTVTGRALRPFTGKPQALKRCLTNLIANAVKFGTRAEV
                     HIEDGADLVVRVRDHGPGIPEKELERVFEPFYRLESSRNRDSGGTGLGLSIARDIAQA
                     HGGSLRLANAAGGGLEATLRLPRRR"
BASE COUNT          369 a          818 c          819 g          381 t
ORIGIN      
        1 gggtatcctc agctctcaga ggcacaatga ccgaacgacc acacgtgttg attgtcgatg
       61 atgatcgcga gatccgcggt ctactcgcgc agtacctgga gaaacacgac ttccgcacca
      121 cctcagtggc ggacggcaag gagatgcgcc gggtgctcga gcgctcgcac gtcgacctgc
      181 tggtgctcga cctgatgttg ccgggggagg atggactgtc gctgtgccgc gagctgcgca
      241 accgctcgca gctgcccatc atcatgctga cggcgcgcgg cgaggacgtc gatcgcatca
      301 tcgggctcga gctcggcgcc gacgactacg tcgccaagcc attcaacccg cgcgagctgc
      361 tcggccgcat caaggcggtg ctgcgccgat cggctcatgc gccgcgcgat cccagtcccg
      421 agagcgtgca cggcttctcc ttcggtggct ggcggctgga caccgtgaca cgcaccctga
      481 cgcattccga ggggcgggag ctggcactca gcggcgctga gtaccggctg ctcgccgtgc
      541 tgctcggcgc cggcaaccgc gtgctgacac gcgcgcagct caccgagctg ctgcgcgggc
      601 gcgatgccga tcccttcgac cgcagtatcg atgtgcgcgt cagccgcctg cggcagatcc
      661 tcggcgatga cgcgcgcgct ccgcagatca tcaagacggt gtacggcgag ggttacgtga
      721 tcggcgtcaa cgtcgcgact gattagcgcc gcgcaagcgc ccatgcgtct ctcgctatgg
      781 ccacaatcgc tgttcggccg actgctggcg gcctcggtgg ccgcggtgct gatcgcccag
      841 gcggtggctc tgctgctgat cgcgcaggag cgcgagcatt tcatgctgca gggcagtgtg
      901 cgcgaatgga cgcgtcgcat cgccgacacg acgctcatgc tcgccccgct gaacgggccg
      961 cagcgcgcgg agctgctcgc gcagctctct gcaacgcagg ccttgcacgc gccgcacatc
     1021 ctgctgcatc gtccggggga gcccggcggc ggggcgcatg agctcgggcg tggcttcctg
     1081 cgactgccac tgctgcggga cttcgagaat gcgctgcgcg aacagctgcg cgccgcgctc
     1141 ggcccgagct acgggatcgc aattgcgccg acgccggagc ccgcaccctc tgcggtcgtg
     1201 ctgccggcgc cgttctacga ggcacacgag ctcgcgctgc accccacgag cgcccagcgc
     1261 tacgacgtga cggtgcgctt tgccgacggc gatgcggtca cctaccgcgt gatgcgcatg
     1321 ccagggggtg cgccgctgcc gcgcaacctg tttctcaacc tgacgctgct ggtgctgttg
     1381 ctggtgatcg tgctctacct ggcagcgcgc aacatcacgc ggccgctgtc ggatctggcg
     1441 cgcgcggccg atagcgtggg acgggattcg cggcccgcgc cgcagctcgc cgagcgcggc
     1501 gcacgcgagc tgcgcgatgc ggcccgcgcc ttcaacacca tgcaggatcg tctacatcgc
     1561 tacctcgata gccgcacgcg ggtgctggcg gccatgtcgc atgacctgaa gacaccgctc
     1621 acgcggctgc gtctgcaggt ggaggcgctc gataacccgc cgatgcaggc gcgtatcggc
     1681 cgcgagctcg atgagatgga gagcatggtg cgcgaggcgc tgtcgctgtt tcgaggtctg
     1741 gacgatggcg agccgccgac cgagttcgat gtggatgagc tgctcgcgca gatccgcggg
     1801 gagttcgccg acatgggggc ggcggtgacg gtcaccggta gggcgctgcg gcccttcacg
     1861 ggcaagcccc aggcgctcaa gcgctgtctc acgaatctca tcgccaatgc ggtcaagttc
     1921 gggacgcgtg ccgaggttca catcgaggac ggtgcggact tggtggtgcg cgtgcgggac
     1981 cacggacccg gcatcccgga gaaggagctc gagcgcgtgt tcgagccctt ttatcggctc
     2041 gagtcctcgc gcaaccgcga cagcggcggc acaggactcg gcctgtcgat cgcccgtgac
     2101 attgcgcagg cgcatggtgg atcactgcgg ctcgccaatg ccgcgggcgg cggactcgag
     2161 gccacgctgc gcctgccgcg gcggcgctga gcagctaccc ggccgcggcg ccgcacgccg
     2221 cgtccacggc ttaacgcagc aggtgcacga cttaacgcaa acgcaaacag ccctaacagt
     2281 cggtcacgat tgttcacggc cgtgggcacc cgccgcccgt tagcgttagc agctgatgcg
     2341 ggcccccaaa tccccccccc gcgaggagac actctatgaa ggccact
//
LOCUS       DATDMP010000664         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1438408, whole genome shotgun sequence.
ACCESSION   DATDMP010000664 DATDMP010000000
VERSION     DATDMP010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438408"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VI195_11635"
     CDS             <1..680
                     /locus_tag="VI195_11635"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY6825086.1"
                     /translation="ELFNVAIQAGAIVAIVLIYWRRLWSLATRLDEAENRDYLAKLLV
                     AFLITALGGFIATRLGWKLPESLLPIALATLIGGVLILIIESYVAGRPQSSVVTWQVA
                     FWVGACQILAAVFPGTSRSASTIFAAMLAGLTLRPAATEFAFLVGIPTMFAATGYELL
                     KVRGQAAGEDWNAFALGFVVSLIVAFIAVKWLLRYVQSHRFTVFAWYRIALGAALLAL
                     IASGMLR"
     gene            complement(691..1074)
                     /locus_tag="VI195_11640"
     CDS             complement(691..1074)
                     /locus_tag="VI195_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825087.1"
                     /translation="MRNLTLAVIVCSASVCWAEEPADTVALQSPIHLDSAADLEQLRK
                     TNPDHYARAVRLIKRAGQLCDPGAPKVQNTDGRDISCATLLLTSNPPKRELSFTLDHT
                     SYVTLVTITTDRPQLVHADQRSSAR"
     gene            complement(1150..>2364)
                     /locus_tag="VI195_11645"
     CDS             complement(1150..>2364)
                     /locus_tag="VI195_11645"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY6825088.1"
                     /translation="VRDRLLGLPVRERDWVVVGATPEELVQLGYQPVGREFPVFLHPT
                     THEEYALARQERKVGPGYRGFTTQFAPSVTLEEDLQRRDLTINAMAEDESGTLIDPYG
                     GRADLEARLLRHVSAAFVEDPVRILRVARFAARFAPLGFRVADETLALMRRISASGEV
                     RTLVPERVWQETERALAAAQPEVFFATLRACDALEVIYPEIDALYGVPQPPRWHPEID
                     TGVHVMLALRYAADQGASGAVRFAVLTHDLGKAQTPKSEWPSHHGHEEFGVPLIEALC
                     DRLKVPNTYRELAILAARYHTRVHRALELRPDTVLTLLESCDALRRPQRFAELLLACE
                     ADARGRTGREHAPYPQVGYLRQALTAAAGVTLTESERRDLEGVEIGEELRRRRRAAIA
                     ALKARTTPTPAR"
BASE COUNT          337 a          779 c          824 g          424 t
ORIGIN      
        1 cggagctgtt caacgtggcg atccaggccg gtgcgatcgt ggccatcgtg ctgatctact
       61 ggcggcggtt gtggagtctc gcgacacggc tcgatgaggc cgagaaccgt gactatctcg
      121 ccaagctcct cgtggcgttt ctgatcaccg cgctcggcgg attcatcgcg acgcgcctcg
      181 gctggaagct gcccgaaagc ctgctgccga tcgctctcgc cacgctgatc ggcggcgtcc
      241 tgatcctcat catcgagtct tacgtcgcgg gccggccaca gagctcggtg gtcacctggc
      301 aggtcgcctt ctgggtcggc gcgtgccaga ttctcgcggc ggtgtttccc gggacatcac
      361 gctcggcctc gaccattttc gccgccatgc ttgccgggct tacgttgcga ccggcggcca
      421 ccgagttcgc ctttctggtg ggcattccca ccatgtttgc cgcgaccggc tacgagttgc
      481 tgaaggtgcg cggccaggcc gcaggcgaag actggaatgc attcgcgctc ggtttcgtgg
      541 tgtcgctgat cgttgcgttc atcgcggtca agtggctgct gcgctatgtc cagtcgcacc
      601 gcttcaccgt ctttgcgtgg taccgcatcg cgctcggagc ggcgctgctg gcgctgattg
      661 cctcgggcat gctccgctga ggagacgcgt tcagcgcgcg gacgagcgct gatcggcgtg
      721 cacgagctgc ggacgatcgg tggtgatcgt cacgagcgtc acgtaactcg tgtggtcgag
      781 cgtgaaggag agctcgcgct tgggcgggtt gctggtgagg agcagcgtcg cgcaggagat
      841 gtcgcggcca tcggtgttct gcaccttcgg cgcaccggga tcgcagagct ggccggcgcg
      901 cttgatgagt cgcacggctc tcgcgtagtg gtcggggttg gtcttgcgca attgctccag
      961 atcggccgca ctgtcgaggt gtatgggtga ctggagagcg accgtgtccg cgggctcctc
     1021 ggcccagcac actgacgcgc tgcacactat gacggcgaga gtcaggttgc gcatggcagc
     1081 tctccggccc atgtatagat gcgccgaagc gagagaagtt tcaagaggcg gcgggttgcg
     1141 gggccgactt cagcgtgccg gcgtgggggt cgtgcgcgcc ttgagggctg cgatggccgc
     1201 ccgacgccgc cggcgcagtt cctcgccgat ctctacgccc tcgaggtcgc gccgctcgct
     1261 ttccgtgagc gtcacgcccg cggctgccgt gagcgcttgc ctgaggtatc ccacctgcgg
     1321 ataaggcgcg tgctcccggc cggtccgccc gcgcgcatcg gcttcgcagg cgagcaacaa
     1381 ctcggcaaag cgctgtgggc ggcgcagcgc atcgcagctc tccagcagcg taagcacggt
     1441 gtccggtcgc aactcgagag cgcgatgcac ccgcgtgtgg tagcgcgcgg cgagaatcgc
     1501 cagctcgcgg tacgtgttgg gtaccttgag gcgatcgcac agcgcttcga tcagcggtac
     1561 gccgaactcc tcgtggccgt gatgactcgg ccattcggac ttcggagtct gtgccttgcc
     1621 gagatcgtgc gtgagcacgg cgaaacgcac cgcgcccgat gcgccctggt cggcggcgta
     1681 tctcagcgcc agcatcacgt gcaccccggt gtcgatctcc gggtgccagc gcggcggctg
     1741 cggcaccccg tagagcgcat cgatctccgg atagatcacc tcgagcgcgt cgcaggcgcg
     1801 cagggtcgcg aagaatacct ccggctgcgc cgcggcgagc gcgcgctcgg tctcctgcca
     1861 gacgcgctcc ggcaccagcg tgcgaacctc gcccgaagcg ctgatgcggc gcatgagtgc
     1921 cagcgtctcg tcggcgacgc ggaatccgag cggcgcgaaa cgtgccgcga aacgtgccac
     1981 gcgcaggatg cgcaccgggt cctcgacgaa ggctgccgag acgtgccgca gcaggcgcgc
     2041 ctcgagatcg gcacggccgc cgtaggggtc gatgagcgtg ccgctctcat cctccgccat
     2101 ggcgttgatg gtgaggtcgc ggcgctggag gtcctcctcc agcgtcacgc tcggcgcgaa
     2161 ctgcgtggtg aagccgcggt agcccggccc gaccttgcgc tcctggcggg cgagcgcgta
     2221 ctcctcgtgt gttgttgggt gcaggaacac cgggaattcg cgccccaccg gctggtatcc
     2281 gagctgtacc agctcctcgg gtgtcgcgcc aaccacgacc cagtcacgct cgcgcaccgg
     2341 cagaccgagc aaccggtccc gaac
//
LOCUS       DATDMP010000665         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_882602, whole genome shotgun sequence.
ACCESSION   DATDMP010000665 DATDMP010000000
VERSION     DATDMP010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882602"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1416
                     /gene="selD"
                     /locus_tag="VI195_11650"
     CDS             370..1416
                     /gene="selD"
                     /locus_tag="VI195_11650"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY6825089.1"
                     /translation="MSTDSVRLSSMAHGGGCGCKLSPAVLATLIGNMSAAQSFKQLLV
                     GTETGDDAAVWQLDERTCLISTTDFFMPMVDDPRDFGRIAATNAISDIYAMGGRPIFA
                     LAILGMPLGKLAPERVREILEGGREVCQSAGIPVAGGHSIDTPEPIYGLAVNGICAPS
                     ELRRNSDAKAGDLVILTKPVGVGIYSAAFKKEALSPADYSDMLATMTMLNQIGAELGK
                     DPGVHAMTDVTGFGVLGHALGMARGSGLRFVLRSESLPLLPRAIELTQQGFVTGASGR
                     NWESYGSAVNLAADCPLWRRQILTDPQTSGGLLVACARERAQTLLGTIRDAGYPSASI
                     IGEMRAGAPGIEVL"
     gene            complement(1450..>2091)
                     /locus_tag="VI195_11655"
     CDS             complement(1450..>2091)
                     /locus_tag="VI195_11655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6825090.1"
                     /translation="ELVRRGHRVTAIDASAAMVARTKQRASATGLEIEACVMDGQNLA
                     FPDASFDAALSVIGVILFPDAVCGLSEMRRVVRPGGRIALVTWTAPEAYELIGELRAA
                     ALSVLGNLPARPLPAQLRFREKEEFRALFAAADLPAAEISEVAAELVAPSPAWLSERL
                     AFAPGMAALLEGFGGAQAEVIAAFRKRLESLRGARRISLAARAYLGSVSVGHA"
BASE COUNT          358 a          704 c          672 g          357 t
ORIGIN      
        1 ccaccgtagc ggccgagcga acatacgggt gggcgccgag gcgcgttttc acgacttctg
       61 cctcggttgc catcgtgacc cgcccgctta cctgaccggt cacgggccgg tcacggggtg
      121 cagtacctgc catgtggcac cgtaaagtgg tcaaccctgg tcccgctgcc ggggctgcac
      181 gtctgcaacg tctgctcgtt cccgtgcgcg cgctgctcag gcgccggctg agtccgccgc
      241 taccgttttt ccgcatcggg gatgtgacgc aaggatgttg tatcatcaag taaacggcgc
      301 tttcgctttg cgcgttcgat aatgagctgc tcgaagcgcc gagaagagcg ccttggagcg
      361 cacgcacaca tgtctacgga tagcgttcgt ctcagcagca tggctcatgg cgggggatgc
      421 ggttgcaagc tctcgccagc cgttctcgcc acgctcatcg gcaatatgag cgctgcgcaa
      481 tccttcaagc agcttctggt cggcaccgaa acgggcgacg atgcggccgt gtggcagctc
      541 gacgagcgta cctgcttgat ctcgacgacc gacttcttca tgcccatggt ggacgacccg
      601 cgtgatttcg ggcgcatcgc cgcgaccaac gccatctccg atatctacgc catgggcggc
      661 aggccgattt tcgcactcgc cattctcggc atgccgcttg gcaagctcgc gccggagaga
      721 gttcgggaga ttctcgaggg cggccgcgag gtatgccaga gcgcaggcat tcccgtcgcc
      781 ggcgggcact ccatcgatac acccgagccc atctacgggc tcgccgtgaa tggcatctgc
      841 gcaccctccg agctacggcg caacagcgac gccaaggccg gggacctcgt catcctcacc
      901 aagccggtcg gtgttgggat ctattccgca gcgttcaaga aggaggcgct gtcgccggcc
      961 gactactcag acatgctcgc gaccatgacg atgctcaatc agatcggtgc cgagctcggc
     1021 aaggacccgg gcgtccacgc catgacggat gtgacggggt tcggcgtgct cggacacgcg
     1081 ctcggcatgg cgcgcggttc cggccttcgc tttgtactga ggagcgagag ccttccgctg
     1141 ctcccaagag cgatcgagct cactcagcag ggcttcgtca ccggcgcctc gggccgaaac
     1201 tgggagagtt atggcagcgc ggtcaacctt gctgcggatt gtccgctgtg gcggcgccag
     1261 atcctgaccg acccgcagac ctccggaggc ctactcgttg cgtgcgcgcg cgagcgggcg
     1321 cagacgctcc tcggcacgat tcgggacgct ggttatccgt ccgccagtat catcggcgag
     1381 atgcgcgccg gcgctcccgg catcgaggtt ttgtagtcca gacgccagcc agggctcagg
     1441 tgaccgacct cacgcgtgcc cgaccgaaac cgagcccaga taggcgcgcg ccgcaagtga
     1501 gatgcgcctg gcgccacgca acgactcgag gcgctttcga aaggctgcga tcacctcagc
     1561 ctgggcgcct ccgaacccct cgaggagcgc tgccatgcca ggagcaaacg caagccgctc
     1621 ggagagccac gcaggcgatg gcgcgaccag ctcggcagcc acttccgaga tctcggctgc
     1681 cggcaagtcg gcagccgcga acaacgcccg gaactcctct ttctccctga agcgcagttg
     1741 cgccggaagt ggccgggccg ggaggttgcc gagcacggag agtgccgcgg cgcgcagttc
     1801 gccgatgagc tcataggctt cgggcgctgt ccaggtgacg agggcgatac gccccccggg
     1861 tctgaccacc cgccgcatct cggacaatcc gcacaccgca tcgggaaaca ggatgacgcc
     1921 aatgacggac agggcagcgt cgaagcttgc atctggaaac gccaggttct ggccgtccat
     1981 gacgcatgcc tcgatctcga gccccgtggc tgacgcccgc tgtttcgttc gcgccaccat
     2041 cgctgcggac gcgtcgatgg cggtgactcg gtggccccgc cgaaccagct c
//
LOCUS       DATDMP010000666         4391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1660121, whole genome shotgun sequence.
ACCESSION   DATDMP010000666 DATDMP010000000
VERSION     DATDMP010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660121"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /gene="purH"
                     /locus_tag="VI195_11660"
     CDS             complement(<1..1453)
                     /gene="purH"
                     /locus_tag="VI195_11660"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY6825091.1"
                     /translation="MSKIQRALLSVSDKSGIADLGRALHALGIELISTGGTAKLLEKE
                     GLPVIEVAAYTGFPEMLDGRVKTLHPKIHGGLLGRRDDPAHVAALRNAGIPGIDLLVV
                     NLYPFQATVADPDCRLEDAIENIDIGGPAMLRAAAKNYGSVAVVVDPDDYARVLEEVR
                     RGAVSAATRFQLARKAFAHTAAYDGAIANYLSCVDASGRRREYPEVLTLQFSKAQDLR
                     YGENPHQSAAFYRDARPPEGSLATYRQLQGKDLSYNNIADADAAWECVKSFGEPACVI
                     VKHANPCGAALGATLHEAYDKAFRTDPVSAFGGIIAFNRALDSFTAAALAKQFAEVIV
                     APRVEPEAQKLLAAKTNLRILEVPLAHGAQQRDFKRVGGGLLVQAADAKALTRDELRA
                     VTKLAPTPQQYDDLLFAWRVAKYVKSNAIVFCAKGATLGVGAGQMSRVDSARIATVKA
                     GAAKLSLAGSVVASDAFFPFRDGLDVVAEAGAVA"
     gene            complement(1453..1662)
                     /locus_tag="VI195_11665"
     CDS             complement(1453..1662)
                     /locus_tag="VI195_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6825092.1"
                     /translation="MKRLLERYFKDLDGEKPTSVYEMVLKNIEKPMIETVLGHAQGNQ
                     SLAAEMLGINRNTLRKKMQQLRIKG"
     gene            complement(1689..2681)
                     /gene="dusB"
                     /locus_tag="VI195_11670"
     CDS             complement(1689..2681)
                     /gene="dusB"
                     /locus_tag="VI195_11670"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY6825093.1"
                     /translation="MKIGAHVLRNAVIVAPMAGITDRPFRTLARKFGAAMAVSEMVSA
                     RPELRASRKTRLRLDHRGETGPISVQIAGADPLMLADAARHNVDHGAQIIDINMGCPA
                     KKVCNVSAGSALLADEALVGRILDAVVRAVPVPVTLKIRTGPHPERRNAMRIAAIAEA
                     AGVQMLAIHGRTRACAFNGQAEYRTIAEVKSRARIPVVANGDIRTPAQAKEVLALTGA
                     DGIMIGRAAQGRPWLFREIAHYLDTGAAAPSVQLTEVAAVLHEHLEGLYSLYGEEHGV
                     RIARKHIGWTVCGLPGGEALRRSANLTADARSQVLAVRGFFQQLAANEPERLAA"
     gene            complement(2730..3794)
                     /locus_tag="VI195_11675"
     CDS             complement(2730..3794)
                     /locus_tag="VI195_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6825094.1"
                     /translation="MPEAQTDLLIRGAGPVGATLALAMREARSRVAILHREATASSAG
                     FRPIALSFASRLILERIGIWSALSPAPIHRILVSQTQGFGRTAIESSDAGVPALGYVV
                     EYHELAGALHAALERSPVALVAEERASRCVIHAEGWSETGEKRYAQDALVARVFFDAG
                     PQGTAYERFTPEGPLALLPLRRGSALIWTLSPEKAHTLAAAPETEFLRALASALPREA
                     GTPIAAEARAPQPLALRVRRTLVGNREVYIGNAAQTLHPVAGQGLNLGLRDAWELAQL
                     LRDAPDPGEPALLARYARRRRVDAQAAIHLTDGLARAFLGTGRAASALRGAMLTALDL
                     LPAPRRFFARRMIYGASAWP"
     gene            complement(3775..>4391)
                     /locus_tag="VI195_11680"
     CDS             complement(3775..>4391)
                     /locus_tag="VI195_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HEY6825095.1"
                     /translation="ACVLHYVSNDAPLSDGDLLLIDAGCELDGYASDITRTFPVNGRF
                     TQAQRDVYELVLSAQLAAIEKVRAGNAWNDPHDAAVRVLAQGLIDLGLLQGSLAETLE
                     KETYKRFYMHRTGHWLGLDVHDAGEYKQSGAWRKLAPGMTLTVEPGLYIRPAEDVPAS
                     LAGIGIRIEDDVLVMAGGCEVLSAEAPKRIDDLEALVRDARSAD"
BASE COUNT          723 a         1431 c         1488 g          749 t
ORIGIN      
        1 cggcgacggc gcccgcttcc gccacgacgt ccagcccgtc gcggaacggg aaaaaagcat
       61 ccgatgcgac cactgatccg gcgagcgaga gtttagccgc gccggccttc acggtggcga
      121 tgcgcgcgct gtccacgcgg ctcatctggc ccgcacccac gccgagcgtc gcgcccttcg
      181 cgcagaagac gattgcgttg gatttcacat atttcgccac ccgccaagcg aaaagcaggt
      241 cgtcgtattg ctgcggcgta ggcgcaagct tcgtcaccgc gcgcagctca tcgcgcgtca
      301 gcgccttggc gtccgccgcc tgcaccagga gaccgccgcc cacgcgcttg aagtcacgct
      361 gctgggcgcc atgggcgagc ggcacctcga ggatgcggag attggtttta gccgcgagga
      421 gcttctgtgc ctcgggttca acccgcggcg caacaatgac ctcggcgaat tgctttgcga
      481 gcgctgcggc agtgaaagag tcgagcgcgc ggttgaaggc gatgattccg ccgaacgccg
      541 agaccgggtc ggtgcgaaaa gccttgtcgt aggcttcgtg cagcgtcgcg ccgagcgccg
      601 caccgcaagg attggcgtgc ttgacgatca cgcaggccgg ctcgccaaag ctctttacgc
      661 actcccatgc tgcgtcggca tcggcgatat tgttatagga aaggtctttt ccctgcagtt
      721 gccgataggt tgcgaggctc ccttccggcg gccgggcatc gcggtaaaag gcggccgact
      781 ggtgggggtt ttcgccataa cgcaggtcct gggccttgga gaactgcagc gtcagcacct
      841 ccggatattc gcgtcgccgc ccggaagcgt cgacgcagga gaggtaattt gcgatcgcgc
      901 cgtcgtaggc tgcggtgtgc gcgaatgcct tgcgggcaag ctggaagcgc gtggccgcgg
      961 agaccgcgcc gcgccgcact tcctcgagca cgcgggcgta gtcatcggga tcgaccacga
     1021 cggcaacgga tccgtagttc ttcgccgcgg cgcgcaacat ggcgggcccg ccgatgtcga
     1081 tgttctcgat cgcatcctcc aggcggcaat ccgggtccgc gaccgtcgcc tggaaagggt
     1141 agaggttcac cacgaggaga tcgatgcccg gaatgcctgc gttccgaagc gcggcaacgt
     1201 gggccgggtc atcgcggcgc ccgagcaggc cgccgtggat cttcgggtgc agggttttca
     1261 cccggccgtc gagcatttcg ggaaaccccg tgtaggcggc gacctcgatg acgggcaagc
     1321 cttctttctc gagaagcttc gccgttccgc cggtcgaaat gagctcgatg ccgagcgcgt
     1381 gcagcgcgcg gccgaggtcc gcgattccgg acttgtccga aacgctgagc aacgcccttt
     1441 ggattttcga cactaccctt tgatgcgcag ctgctgcatc ttcttgcgca aggtgttgcg
     1501 gttgatcccg agcatttccg ccgcgagcga ctggttgccc tgggcgtggc ccagcaccgt
     1561 ttcgatcatg ggcttctcga tgttcttcag caccatctcg tataccgacg tgggcttttc
     1621 gccgtccagg tccttgaagt agcgctcaag caggcgcttc acgcaatccg cgagctcgtt
     1681 gccgcgactc atgccgcgag cctctcgggc tcgttcgcgg cgagttgctg gaagaacccc
     1741 cgcacggcga gcacctgcga gcgggcgtcg gccgtgagat tcgccgagcg gcgcagcgct
     1801 tcgccccccg gcagcccgca tacggtccac ccgatgtgct tgcgggcgat gcgaaccccg
     1861 tgctcttcgc cgtacaggct gtacaagcct tccagatgct cgtgcagcac cgcagcgact
     1921 tcggtcagct gcacggaagg agccgccgcg ccggtgtcga ggtagtgagc gatctcgcga
     1981 aagagccaag ggcgcccttg cgctgcgcgg ccaatcatga tgccgtcggc acccgtaagc
     2041 gcaagaacct ctttggcctg cgccggcgta cggatgtcgc cgttggcgac gaccgggatg
     2101 cgcgcacggc tcttcacttc ggcgatggtc cggtattcgg cttgcccatt gaaggcacac
     2161 gcgcgcgtgc gcccgtgaat cgccagcatt tgcacgcccg cggcttcagc gattgcagcg
     2221 atgcgcatcg cattgcgtcg ttccgggtgc ggcccggtgc ggattttcag cgtgacgggc
     2281 accggtaccg cccgcaccac tgcatcgagg atccggccca ccagggcttc gtcggcgagc
     2341 agcgccgagc cggcgctcac attgcagacc tttttcgccg ggcagcccat gttgatgtcg
     2401 atgatctgcg cgccgtgatc cacattgtgg cgcgcggcgt cggcaagcat cagcggatct
     2461 gcgccggcga tctgcaccga aatcggaccg gtctcgccgc ggtgatcgag ccgcaggcgg
     2521 gtcttgcgcg aggcccgcag ctcggggcgc gcggaaacca tctcggagac cgccatggcc
     2581 gcgccgaatt tgcgggcgag cgtccggaac gggcggtccg tgattccagc catcggggca
     2641 acgattactg cgttgcgcag aacgtgcgcg ccaatcttca tcaggcggcg tggcgttacg
     2701 cgagcagaat tagggaaacg gattctagct tacggccaag ccgaagcgcc atagatcatg
     2761 cgacgcgcga aaaaacgacg cggcgcaggc agcaggtcca gcgccgtgag catggcgccg
     2821 cgcagcgcgg aggctgcccg gccggtgccc aggaatgcgc gcgcgaggcc gtccgtgagg
     2881 tggatcgccg cctgcgcgtc gacgcgccgc cgccgcgcgt aacgggcgag gagcgcgggt
     2941 tcgcccggat ccggcgcgtc ccgcaaaagc tgcgccagtt cccaggcatc gcgcaggccg
     3001 agattcaggc cctggccggc gaccggatga agggtttgcg cggcgttgcc tatatagacc
     3061 tcgcggttgc cgacgagggt gcggcgtacg cgcagcgcca gcggttgggg cgcgcgtgcc
     3121 tcggcggcga tgggtgtgcc cgcttcacgc ggaagagctg acgcgagggc gcgaagaaac
     3181 tcagtttcag gggcggcggc gagggtgtgc gctttctccg gcgacagcgt ccatatgagc
     3241 gctgaccccc ggcgcaacgg tagcagcgcg agcggtcctt cgggggtgaa tcgctcatag
     3301 gcagtgccct gcggaccggc gtcgaagaag acgcgcgcga cgagggcatc ctgcgcatag
     3361 cgcttctctc cggtttccga ccacccctcg gcgtgaatga cgcagcggct cgcgcgctct
     3421 tcggcgacga gtgctaccgg cgagcgctcg agcgccgcat gcagcgcgcc agcaagctca
     3481 tggtattcga cgacatagcc cagggcgggc actccggcat ccgaagactc gatcgcggtg
     3541 cgaccaaatc cctgagtctg cgaaaccagg atgcgatgaa tcggggccgg cgacaacgcg
     3601 ctccatatgc cgatccgctc aaggatcagg cggctcgcga acgatagcgc tatgggcctg
     3661 aagcccgctg acgaagcagt cgcttcgcga tggaggatgg cgacgcgcga gcgcgcttcg
     3721 cgcatggcga gcgcaagcgt ggcgcccacc gggccggcac cgcgaatcag gaggtcagtc
     3781 tgcgcttcgg gcatcgcgca cgagtgcctc gaggtcgtcg atgcgcttgg gcgcctcggc
     3841 gctcaggact tcgcagccgc ccgccatgac gagcacatcg tcttcgatgc gtattccaat
     3901 tccggcgagc gaggccggca cgtcttcggc tggccggatg tagaggcccg gctccaccgt
     3961 gagcgtcatt ccgggcgcga gcttgcgcca cgcgcctgac tgcttgtact cgccagcgtc
     4021 gtgcacatcc agcccgagcc aatgcccggt gcggtgcata tagaagcgct tgtaggtttc
     4081 cttttccagc gtttccgcga gactgccttg aaggaggccc aggtcgatca agccctgcgc
     4141 gagcacgcgc actgcggcgt cgtgcggatc gttccacgcg tttccagcgc ggactttttc
     4201 tatcgccgcg agctgcgccg acagcaccag ctcatatacg tcgcgctgcg cttgcgtgaa
     4261 gcgcccgttc accgggaagg tgcgcgtaat gtcggaagcg tagccatcga gctcgcatcc
     4321 ggcgtcgatg agaagcaaat cgccgtcgga aaggggcgca tcgttgctca cgtagtgcag
     4381 cacgcaggcg t
//
LOCUS       DATDMP010000667         3222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1106136, whole genome shotgun sequence.
ACCESSION   DATDMP010000667 DATDMP010000000
VERSION     DATDMP010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106136"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VI195_11685"
     CDS             complement(<1..1680)
                     /locus_tag="VI195_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY6825096.1"
                     /translation="MSLAGTERRRHLLTASIGTALTLVMGLALIWGFRLATHMRSNII
                     ALQTASTLQTYPEEIAHQLNALRDRLEVRAYSGQALADLQASVKRFDHELRLLGGSVD
                     ADSPQLGHALLLWHQYAPVLEPVVSFNGQPYVDSDTAGSSLSREGREHYAAVKRAQLF
                     ASENARPLQTQLANLASGLERTSSEAATRLRSLLMGGVFAALVLAVVAAYFQLSRARH
                     ERAAREAQEQTRDILKTVREGFFLLDAHYRIGAVWSEALTRMFSRNDFAGLTFEELLA
                     DLVPQATLATAMKYIKLLWGDRAHENLMKSINPLGQLEITMDNGHGGKETRYLQFDFH
                     RVMGPEGIKHVLCAVGDITSSVLLARELHESQENANAQLDMMVGMMHIDPLQLVSFLD
                     TAETGLKLVNTILKEPARTDAEFRKKLAGLFRELHTIKGEASALNLKSIANRVHTLED
                     MVGECKKKPELSGNDFLPMVLKLDELLAHLRNVREMAARLTVLKDTVPGSAAAAADMA
                     AAPPAAAPAPTPAATTPVMTAASGSAAHGSAAAAAGRPSGAERAARRVEELS"
     gene            complement(1697..>3222)
                     /locus_tag="VI195_11690"
     CDS             complement(1697..>3222)
                     /locus_tag="VI195_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY6825097.1"
                     /translation="LLLGIAGASLVALAGAAVWYFGHGRQLQAPAAAKLAAPPASAPA
                     SSAEVPAPAAPTADNSIVQGKVDELLEKARLAMHERRFTEPAGDNALLYYRSAAAANA
                     ASGEARDGLQRVAAVLASRFDEAMSAARLEEAAQTLANFKSAAPTDARIGTFEQRLYA
                     AQITRALNDGNPERATQYLRQAQQATSIPAEQITRWRADIARRQEDAKVQRLAALVED
                     RIRAGALSGPDDSAKAYLLQLQAAAPGNPNTQRVAHELIGADLHKARDAAAARNPMEQ
                     ERWLTEARAVGLKPAEAAAFQRELASARQKASQAEGERLAQLARERMRDGRLTDPPQD
                     SAAWYLTQLQTLDAGSSALADASRELAGRLLDRARSTVLAGKAPDADLAQARRWGADP
                     KDVTAVQQLQPPKGKGATLDAAALAANLKRLRSTPPEYPQAAISQHITGSVTLEYTVD
                     SHGEPRDIHVVEATPPGVFDQAAANAVKRWRYAPVVVDGTAVDVPAVRTRVRFELPK"
BASE COUNT          444 a         1094 c         1131 g          553 t
ORIGIN      
        1 cgagagctcc tcgacgcggc gcgcggcgcg ctccgcgccg gacggcctgc cggcggccgc
       61 cgctgccgag ccgtgcgctg ccgagcccga agcggccgtc atgacaggcg tggtcgcggc
      121 gggcgttgga gccggcgccg cagcgggcgg cgcggctgcc atatccgccg ccgccgcagc
      181 gctccccggc accgtgtcct tgagcacggt cagccgggcg gccatctcgc gcacgttgcg
      241 caggtgcgcg agcagctcgt cgagcttcag caccatcggc aggaagtcgt tgcccgaaag
      301 ctcgggcttc ttcttgcact cgccgaccat gtcctcgagc gtgtgcacgc ggttggcaat
      361 gctcttgaga ttgagcgccg atgcctcgcc cttgatggtg tgcagctcgc ggaacagacc
      421 cgcgagtttc ttgcggaatt ccgcatcggt gcgcgccggc tccttgagaa tggtgttgac
      481 gagcttcagc ccggtctcgg cggtgtcgag gaacgacacc agctgcagcg ggtcgatgtg
      541 catcatgccc accatcatgt cgagctgggc gttggcgttt tcctgcgact cgtgcagctc
      601 gcgcgccagc agcacgctcg aggtgatgtc gccgaccgcg cacaacacgt gcttgatgcc
      661 ttccggaccc atcacgcggt ggaagtcgaa ctgcaggtaa cgggtctcct tgccgccgtg
      721 accgttgtcc atggtgatct cgagctggcc gagcgggttg atgctcttca tcaaattctc
      781 gtgggcacga tcgccccaca gcagcttgat gtacttcatg gcggtggcga gcgtcgcttg
      841 cggcaccagg tctgcgagca actcctcgaa ggtgagaccg gcgaagtcat tgcggctgaa
      901 catgcgcgtc aaggcttcgg accagaccgc gccgatgcga tagtgggcat cgagcaggaa
      961 gaagccctcg cgcaccgtct tgaggatgtc gcgggtctgc tcctgggcct cgcgcgcggc
     1021 gcgctcgtgc cgcgcgcgtg acagttggaa gtaggcggcc accactgcca gcaccagcgc
     1081 ggcgaacacg cctcccatca gcagcgagcg caggcgcgtc gcggcttccg aggaagtgcg
     1141 ctcgaggcca ctcgcgagat tggcgagctg cgtctgcagc ggccgggcgt tctcgctggc
     1201 gaacagctgt gcgcgcttca cggcggcgta gtgctcgcgc ccctcgcgcg acagcgagct
     1261 gccggcggtg tcggagtcca catacggctg gccgttgaac gacaccaccg gctcgagcac
     1321 cggcgcgtac tgatgccata gcagcagtgc atggccgagc tgcggcgagt ccgcatcgac
     1381 cgagccgccg agcaggcgca gctcgtggtc gaagcgcttg acgctcgcct gcagatcggc
     1441 gagcgcttgt ccggagtagg cgcgtacctc gagccggtcg cgcagcgcgt tcaactggtg
     1501 cgcaatctcc tcgggatagg tctgcagcgt gctcgccgtc tgtagcgcga tgatgttgct
     1561 gcgcatgtgc gtggcgagcc tgaagcccca gatcagcgcg aggcccatga ccagcgtcag
     1621 ggccgtgccg atgctggcgg tgaggaggtg gcggcggcgc tctgtgcctg caaggctcat
     1681 cggctgggtc tctcggttat ttgggcagct cgaagcgcac gcgtgtcctg acggccggca
     1741 cgtcgactgc cgtaccgtcc accaccaccg gtgcataacg ccagcgcttc accgcattgg
     1801 ctgcggcctg gtcgaacact cccggcggtg ttgcctcgac cacatggatg tcgcgcggct
     1861 cgccatggct gtcgacggtg tactcgagcg tgacgctgcc ggtgatgtgc tgggagatcg
     1921 ccgcctgcgg atattccggc ggtgtggagc gcagccgctt caggttcgcg gcgagcgccg
     1981 ccgcatcgag cgtcgcgccc ttgcccttcg gcggctgcag ctgctgcacg gcggtcacat
     2041 ccttcgggtc cgcgccccag cgccgtgcct gggcgaggtc ggcatcgggc gccttgccgg
     2101 cgagcaccgt gctgcgggcg cgatcgagaa gcctccccgc gagctcgcga ctcgcgtcgg
     2161 cgagcgcgct gctgccggcg tcgagcgtct gcagctgcgt gagataccac gcggcactgt
     2221 cctgtggcgg gtcggtcagc cgtccgtcgc gcatgcgctc gcgcgccaat tgcgccaggc
     2281 gctcaccctc agcctgggag gctttctgcc gcgcactcgc cagctcgcgc tgaaacgccg
     2341 cggcctcggc cggcttgagt ccaacggcgc gcgcctcagt caaccagcgc tcttgttcca
     2401 tggggttgcg tgccgcggcc gcatcgcgcg ccttgtgcag atccgcgccg atcagctcgt
     2461 gcgccacgcg ctgcgtgttg ggattgccgg gcgcagcagc ctgcaattgc agcagatagg
     2521 ccttggcact gtcgtccggg ccgctgagcg cgcccgcgcg gatacggtcc tcaaccagtg
     2581 ctgcgaggcg ctgcactttc gcgtcctcct ggcggcgggc gatatcggcg cgccagcgcg
     2641 tgatctgctc cgccggtatc gaagtcgctt gctgcgcctg gcgtaggtac tgcgtggcac
     2701 gctcggggtt gccatcgttg agcgctcgcg tgatctgcgc cgcatacagg cgctgctcga
     2761 aagtgccgat ccgggcatcc gtgggagccg cggatttgaa gttggcgagc gtctgcgcag
     2821 cttcctccag ccgcgcggcg ctcatcgcct catcgaaacg actcgcgagt acggcggcga
     2881 cacgttgcag gccgtcgcgg gcctcgccac tcgcggcatt cgccgcggcc gccgagcggt
     2941 aatacagcag ggcgttgtcg ccggccggct cggtgaagcg ccgctcgtgc atggcgaggc
     3001 gcgccttctc gagcagctca tccaccttac cctgcacgat cgagttgtcg gcggtcggag
     3061 cagcgggagc ggggacttca gcagagcttg ccggcgcact ggctggcggc gctgccagct
     3121 tggctgccgc cggtgcctgg agttgcctgc cgtggccgaa ataccagacg gccgcgccgg
     3181 cgagcgcgac caggctcgcg ccagcgatgc cgagcagcag ca
//
LOCUS       DATDMP010000668         3239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_772005, whole genome shotgun sequence.
ACCESSION   DATDMP010000668 DATDMP010000000
VERSION     DATDMP010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772005"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VI195_11695"
     CDS             complement(<1..225)
                     /locus_tag="VI195_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY6825098.1"
                     /translation="MSAAPDLATLTQFVYDEARLIDEKRFEEWYELFTDDGYYWMPLT
                     RGQPDGENHTSLLYEDKLLLRVRIDRLKNPH"
     gene            complement(222..1541)
                     /locus_tag="VI195_11700"
     CDS             complement(222..1541)
                     /locus_tag="VI195_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY6825099.1"
                     /translation="MSYRNQPDAIRALVREDAVHRDVYRSQEIFDLEMERLWRNTWIY
                     VGHDSQVPNAGDYYSTEIARQPVIMLRDEGGKVRVLMNRCAHKGARLVSAASGRCENR
                     LLRCPYHGWTYRLDGSIRTIPMKSGYEGTALGESPNSRGIVNVPHVENYRGFVFVRLS
                     ERGVGFREYFGDSLTSIDNMVERSPEGRLEVAGGVLRFMHDSNWKMFVENLNDTMHPM
                     VAHESAAGTAKKLWADKPEGTPKPMAIEQFLPFVNGYSFFDQMGVHIYENGHSYTGVN
                     FSIHSAYSAIGEYETLMEKSYGAERARAILGEVRHNTVYYPSLTIKGAIQAIRVARPI
                     APDKTLIESWTFRLAGAPPQLLRRTLMYTRLINAPTSVVGHDDRHCYRAIQEGLAAEG
                     NDWVSLHRNWTARERTGLAGNYNGTSEVSMRGQYRAWQQFMAPETRP"
     gene            complement(1560..3038)
                     /locus_tag="VI195_11705"
     CDS             complement(1560..3038)
                     /locus_tag="VI195_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEY6825100.1"
                     /translation="MWRPPERIRHNPRPGRWPSAAEAAASRLFSPVRVGTVTLEQRTW
                     VPAMVPWRASAEGVVTPEVLEWYRCFARGRPGGLVVEATGIRDVPSGPLLRIGDDRFL
                     PGLRTLVDVVREASGGATRLFIQVIDFLSIRRRPDPARFLGEFLKISARHRAAFGHGD
                     ASDLEVRNWLRALPPADLARVLEPRELEALEFGARERVTDTHLEHIRALPRTLPDLFA
                     AAARRARTAGFDGVELHFAHAYTMASFLSRLNERTDGYGGDGASRVRLPLEVLAAVRA
                     AVGPDYPVGCRFLAEECIAGGSDAEDAVLFGREFARAGMDFLSLSRGGKFDDARQPKV
                     GEAAYPYTGRSGYECMPSYYSDARGPYGRNLPPTAKVRAAVRAEGLETPVIAAGGIHS
                     FEQAEGVLASGQADIVGFARQALADPDWFRKVRSGRGAEVRLCLYTNYCEALDQRHRQ
                     VTCELWDRIGLNEPGLKLSSDGKRRLTPPSWSETSEVSITNL"
     gene            complement(3044..>3239)
                     /locus_tag="VI195_11710"
     CDS             complement(3044..>3239)
                     /locus_tag="VI195_11710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6825101.1"
                     /translation="EQVVRLTWYLTSRAEYYAELAEIGTAYREVMGKHFPTMSVVQVV
                     ALMEAEAKVEIEATAVIPPD"
BASE COUNT          536 a         1066 c         1064 g          573 t
ORIGIN      
        1 gtgcgggttc ttgaggcgat cgatgcgcac gcgcagcagc agcttgtcct cgtagaggag
       61 cgaggtgtgg ttctctccgt ccggctggcc ccgggtgagc ggcatccagt agtagccatc
      121 gtcggtgaag agctcgtacc actcctcgaa gcgcttctcg tcgatgagcc tcgcctcgtc
      181 gtacacgaac tgcgtcagcg tcgcgagatc cggagccgcg ctcatggacg cgtttccgga
      241 gccatgaact gctgccaggc acgatactgg ccgcgcatcg aaacctcgct ggtgccgttg
      301 tagttgccgg ccagcccggt gcgctcgcgc gcggtccagt tgcggtgcag gctcacccag
      361 tcattgccct cagcggccag accctcctgg atggcgcggt agcaatggcg atcgtcgtgc
      421 cccacgaccg aggtgggggc gttgatcagg cgtgtgtaca tgagcgtgcg gcgcaataac
      481 tgtggcggcg cgccggccag gcggaaggtc caactctcga tcagtgtctt gtcgggagcg
      541 atcgggcgtg ccacgcggat ggcctggatc gcgcccttga tggtgaggct cggatagtag
      601 acggtgttat ggcgcacttc gcccaggatg gcgcgcgcac gctccgcgcc gtagctcttc
      661 tccatcagcg tctcgtactc gccgatggcg gagtaggcgg agtgaatgga gaagttgacc
      721 cctgtgtagc tgtggccgtt ctcgtagatg tgcacgccca tctgatcgaa gaagctgtag
      781 ccgttgacga agggcaggaa ctgctcgatg gccatgggct tcggcgtgcc ttccggcttg
      841 tcggcccata gcttcttggc ggtcccggcc gcggactcgt gcgccaccat cgggtgcatg
      901 gtgtcgttca ggttctcgac gaacatcttc cagttgctgt cgtgcatgaa acgcagcacg
      961 ccgcccgcca cttccaggcg cccttcgggc gagcgctcga ccatgttgtc gatcgaggtc
     1021 agcgagtcgc cgaagtactc acggaacccg acaccccgtt cactcagccg tacaaacacg
     1081 aagccgcgat agttctccac gtgcggcaca ttgacgatcc cgcggctgtt gggactctcc
     1141 ccgagcgcgg tgccctcgta gccgcttttc attggaatgg tgcggatcga gccatccagg
     1201 cgataggtcc agccgtgata gggacaccta agtagccggt tctcgcaccg cccgctcgcc
     1261 gcgctcacca ggcgcgcacc cttgtgggcg cagcggttca tgagcacgcg caccttaccg
     1321 ccctcatcgc gcaacatgat cacgggctgc cgcgcgattt cggtgctgta gtagtcgcca
     1381 gcgttcggca cctggctgtc gtgaccgacg tagatccagg tgttccgcca cagtcgctcc
     1441 atctccaggt cgaagatctc ctggctgcgg tagacgtcgc ggtgcacggc atcctcgcgc
     1501 accagcgcgc ggatcgcatc gggttgattc cggtagctca cgataggact tctctgtgac
     1561 tacagattcg ttatggatac ttcgctcgtc tctgaccagg acggcggagt cagacgccgc
     1621 ttgccgtcgc tcgagagttt aaggccgggc tcattgaggc cgatacggtc ccacagctcg
     1681 caagtgacct gtcggtggcg ttggtcgagt gcctcgcaat agttggtgta caggcacagt
     1741 cgcacctcgg ctccgcgtcc gctgcgcacc ttgcggaacc agtcggggtc ggcgagcgcc
     1801 tgacgcgcga agcccacgat atccgcctgg ccggaggcga gcaccccttc ggcctgctca
     1861 aagctgtgaa tgccgcccgc agcgatcacc ggagtctcca ggccctcagc gcgtacggcg
     1921 gcgcgaacct ttgccgttgg cggcaggttg cgcccgtaag gaccccgggc atcggagtag
     1981 taggacggca tgcactcgta gccactgcgc ccggtatagg ggtaggcggc ctcgccgacc
     2041 ttcggttgtc gggcgtcatc gaacttaccg ccacgcgata gcgatagaaa gtccattccg
     2101 gcgcgcgcga attcgcgccc gaagagcacg gcatcctcag catcgctgcc gccggcgatg
     2161 cactcttccg cgaggaagcg gcagccgacc ggatagtccg gacccaccgc cgcgcgcacc
     2221 gccgccaaca cttcgagggg caagcgcacg cggctcgcgc catcgccccc gtaaccatcg
     2281 gtgcgttcat tcaaacgcga gagaaaggag gccatggtgt aggcatgcgc gaaatgaagc
     2341 tccacgccgt cgaagccggc cgtgcgcgca cggcgcgcgg cggccgcgaa gagatccggg
     2401 agcgtacgcg gcagcgcgcg aatgtgctca agatgggtat cggtgacgcg ttctctcgcg
     2461 ccgaactcga gcgcctcgag ctcgcgcggc tcgaggacgc gcgccaggtc ggcaggtggc
     2521 agtgcgcgta accagttgcg cacttctaga tcgcttgcgt cgccgtgccc gaaggcggcg
     2581 cgatggcgtg cgctgatctt caggaattct cccaggaagc gcgccgggtc gggtcggcgg
     2641 cggatgctga ggaagtcgat gacctggata aagaggcgcg tcgccccgcc actggcctca
     2701 cgcaccacat cgaccagcgt gcgtaatccg ggcaggaagc ggtcgtcgcc aatgcgcagc
     2761 agcggcccgc tcggcacgtc gcgaatgccg gtggcttcta caacgagccc cccggggcgg
     2821 ccgcgcgcga agcagcgata ccactcgagc acctcgggtg ttaccacgcc ttctgcacta
     2881 gcgcgccagg gaaccatggc cggcacccag gtgcgctgct ccagtgtcac tgtgccgacc
     2941 cgcaccgggg agaaaagccg ggaagcggct gcctctgccg cgctcggcca acgcccgggc
     3001 cgcgggttgt gacggatgcg ctccggggga cgccacatcg acgctagtcc ggcgggatga
     3061 ccgcggtcgc ctcgatctcg accttcgcct cggcctccat gagtgccacg acctgtacca
     3121 cactcatggt cgggaaatgc ttgcccatca cctcccggta ggcggtgccg atctcggcga
     3181 gctcggcgta atactcggcg cggctcgtga ggtaccacgt gaggcgtacc acttgctcg
//
LOCUS       DATDMP010000669         4456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1771689, whole genome shotgun sequence.
ACCESSION   DATDMP010000669 DATDMP010000000
VERSION     DATDMP010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771689"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VI195_11715"
     CDS             <1..371
                     /locus_tag="VI195_11715"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEY6825102.1"
                     /translation="LCSPVARQRVIAMRTMIKLALALSGGAGFLSTQPSFAETLRCGA
                     VLIQPGDDARYVLEKCGEPASAPASSDAMPEGGITVYQIGIMRADRWRYHRGPGLFPA
                     VVTIADDGRVADIQFEQVRD"
     gene            complement(393..1397)
                     /locus_tag="VI195_11720"
     CDS             complement(393..1397)
                     /locus_tag="VI195_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6825103.1"
                     /translation="MDALSDVLRAVRLNGAIFFDVHASDPWAAETPAGNQIVGRMFPA
                     AEHLICYHAITHGTCWAAVADEPPMMLSAGDVVVIPHGHAHVLSSAPGLRGAQDVSRY
                     QAPSELELPVVISMGGGTTESAGFVCGFLGCDARPYNPLLSSLPHVFKVSDQVGGPLG
                     SYVQIALTESKRPRIGSESVLGRLSELMFVDVIRRYLETLPADRLDWLAGLRDPYIGR
                     ALAEIHREPARHWTLESLARQVAMSRSAFAEHFSRFVGTPPMQYLTNWRMQIAANHLL
                     STTDSVNAVANRVGYDSEAAFSRAFKKVVGAPPSEWRKQRATAFAGTPRAVHPDEDAA
                     "
     gene            1518..1943
                     /locus_tag="VI195_11725"
     CDS             1518..1943
                     /locus_tag="VI195_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY6825104.1"
                     /translation="MSTLSGRCFCGEVQFEVTGSPVAMGYCHCESCRHWSAGPVNAFS
                     LWQPESLTITRGAASIGTYNKTPGSYRKWCTRCGGHLFTEHPTMALIDVYAATLPQLK
                     FEPALHVNYRESVLRIKDGKPKMQDMPKEMGGSGVVVAE"
     gene            2377..>4456
                     /locus_tag="VI195_11730"
     CDS             2377..>4456
                     /locus_tag="VI195_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6825105.1"
                     /translation="MLLLERDKPLAQLTRWHEASEAHAGCVVLVTGEAGIGKTAMVSE
                     FIRRRGDAVRVFWGACDPLSTPQPLGPLFDVSRQVGGALLEAIAQGTNRERIFAATIE
                     VLQRSAKPVVLVIEDMHWADDATLDLMKYLGRRIGRTRATLIATYRTDEIHPRHPLQV
                     AIGLLPGETVRRLPLAALSAAAVGELAQKLGRSAAGLYAATGGNPFYVTELLAAPPGD
                     IPNSVRDAVIARVAHLSPGARRLAELVAAVPGHTEAWLIQAVLQASEAAVEECTLSGM
                     MRTADDALAFRHELSRRAVESSLTPSQARALHECILQALQRKNRCEVSESRLVHHAVR
                     AGDIAAVLEFAPRAAERSAGVGAHHEAIAYYRTALGHAERMSPELRGTLLDSLAYECF
                     LTSEIDAAIVAQRDALAIWRSSENAKRAGDTMRSLSRLHWVRGRGAEAHRYAAEAVAA
                     LEPLGPSDELAMAFSNMAQLAVLASHDDAAIEWGERAIELASRLGNDEILAHALNNVG
                     AARTRATGDAGWRELERSLELSLRRGFQEHVARAHTNLAALAIGTRDYRAAGPHLRAG
                     LAFCAEHELYMAEGYLRALRARVNFEQGAWSDAIEEAQGLLREPGLSIAARIPALTVL
                     GRVLARRGTEGALAPLEEARMLAMATGEPQRIVSVAAARAEHAWLRGDLAAVGRELGS
                     VLEYALRDRDH"
BASE COUNT          774 a         1452 c         1486 g          744 t
ORIGIN      
        1 aactatgttc ccccgtggcg cggcaacggg tgatagccat gcgaacaatg atcaagcttg
       61 cgctggcgct gagcggcggc gcaggcttcc tgtccacaca accttctttc gccgagacgc
      121 tacgctgcgg cgcagtgttg atccagccgg gcgacgatgc ccgctatgtg ctggagaaat
      181 gtggtgagcc tgcatccgcg ccggcgagca gcgatgcgat gcccgagggc ggcatcacgg
      241 tatatcagat cggtatcatg cgcgcggatc gctggcgcta ccatcgcggc cccgggctgt
      301 tcccggcggt cgtgaccatc gccgatgacg ggcgcgtcgc ggatatccaa ttcgagcagg
      361 tccgggactg acacccgcaa gggccgtccg ggctaagcgg catcttcgtc cgggtgcact
      421 gctctcggcg ttccggcgaa cgcagttgcc cgctgcttgc gccattcgct cggcggagca
      481 cccaccactt ttttgaacgc ccggctgaac gcagcttctg aatcgtagcc gacacgattc
      541 gcgaccgcgt tcacgctgtc ggtagtgctc agcagatgat tggcggcgat ctgcatgcgc
      601 cagttcgtca gatactgcat cggcggtgta ccgacgaatc ggctgaagtg ctccgcgaac
      661 gcggaacgtg acatggctac ctggcgcgcc agcgactcca gcgtccagtg acgcgccggt
      721 tcgcggtgaa tctcggcaag tgcgcgcccg atgtagggat cgcgcagtcc ggcgagccaa
      781 tcgaggcgat cggcgggcag tgtctcgagg tagcgacgaa ttacgtccac gaacatgagc
      841 tcgctcaagc gcccgagcac actctcgctg ccgatacgtg gccgtttcga ctccgtcagg
      901 gcgatctgca cgtacgatcc aagcggaccg cccacttgat cgcttacttt gaaaacgtgc
      961 ggcagcgagc tcagcaacgg gttatagggt cgtgcatcgc agccgaggaa gccacacaca
     1021 aaaccggccg actccgtggt tccgcccccc atcgagatga ccaccggcag ctccagctcg
     1081 ctcggtgcct ggtagcggct gacatcctgt gcgccgcgca acccgggggc gctcgagagc
     1141 acgtgcgcat ggccatgcgg gatgaccacg acgtccccgg cgctcagcat catcgggggt
     1201 tcgtcggcga ccgccgccca gcaggtaccg tgcgtgatgg cgtgatagca gatcaggtgc
     1261 tcggccgccg ggaacattct gccgacgatc tgattgcccg ccggggtctc cgccgcccag
     1321 gggtcggagg cgtgaacatc gaaaaatatc gccccgttga ggcgaaccgc gcgcaacaca
     1381 tccgacaaag catccatcag gcactccaca gacgatcgag catgaaaggt ggacgaccgg
     1441 tcattcgaag ctcgcgcggc cgtcgctaca cttgccgctg aacaatcggc ttcattttac
     1501 tcgagggtct cgtattcatg agtactttgt cagggcgctg cttttgcgga gaagtgcagt
     1561 tcgaagtcac cgggtctccc gtcgcgatgg gctattgcca ctgcgagtcc tgccgccact
     1621 ggtcagccgg accagtgaat gcgttctcgc tgtggcagcc cgagtcgctc accatcaccc
     1681 ggggcgcagc gagcatcggc acttacaaca agaccccggg cagttaccgg aaatggtgta
     1741 cccgctgcgg cggccacctg ttcaccgaac atcccaccat ggccttgata gatgtttatg
     1801 cggccacgct gccacaactg aaattcgagc cggcgctgca cgtgaactac cgggagagcg
     1861 tgctgcgcat caaggacggc aaaccgaaga tgcaggacat gccgaaggag atgggcggtt
     1921 cgggcgtggt agtggccgag taaagttcgg gtcgtgcggt gcatcgacgt cggccaccgc
     1981 acgtgcaatc gcctggccta cgcaactgcg atcgtagcct accggaccgt atgctgcgca
     2041 caccgtgcgc gcgggcttgc gaatgcggcg atacaactcc tgcgccgcgg caggccgcgc
     2101 cacatcgaga tcggcgaatt cacgacctcg ctgcgcagat cagcggcggg cgccgcagcg
     2161 attgcagccg gcgagattgc atgaagggtc aaggcctcga tgagggtagt ggcgatatgt
     2221 ttcacgtgag catctcctcc ccggcagttt ccggtggaga gcagactacg gcggccggtg
     2281 gcgcgagcca tcggtgcctc gccccatctt tctgctgcca gtcacctaaa aaatccattg
     2341 cgccgggaac ggcatcccgc tcacgaggaa acggtcatgc tccttctcga gcgcgataag
     2401 ccgctcgcgc agctgacgcg ctggcatgag gcgagcgagg cccacgcagg ctgtgtggtg
     2461 ttggtcaccg gcgaggccgg catcggcaag acggcaatgg tcagcgagtt catacgccgg
     2521 cgcggcgatg cggtgcgcgt gttttggggc gcgtgcgacc cgctgtccac gccacagccg
     2581 ctcggtccac tgttcgatgt cagccgtcag gtaggcgggg cgttgctcga ggcgatcgcg
     2641 cagggcacca accgcgagcg tatcttcgca gcgaccatcg aggtgctgca gcggtccgcg
     2701 aagccggtgg tactggtgat cgaagacatg cattgggcgg atgacgccac gctcgacctc
     2761 atgaagtatc tcggtcggcg tatcggacgt acacgcgcca cgctgatcgc cacttatcgc
     2821 accgacgaga ttcatccgcg ccatcctctg caggttgcta ttggattgct gccgggtgag
     2881 acggtgcggc gcttgccgct tgcggcactc tcggcagcag cagtcggcga gctggcgcag
     2941 aagctcgggc gctccgcggc cggcctgtac gccgctaccg gcggcaatcc gttctacgtg
     3001 accgagctgc tggccgcacc gcctggcgat atacccaact cggtacgcga cgcggtgata
     3061 gcgcgcgttg cccatctctc gcctggtgcg cgccggctcg ccgagctggt ggccgccgtg
     3121 cccgggcaca ccgaggcatg gctgatccag gcggtgttgc aggccagcga agccgcggtg
     3181 gaggaatgca cgctgagcgg catgatgcgc actgcagacg acgcgcttgc cttccgccac
     3241 gaattgtcac gccgcgccgt ggagagctcg ctcacgccct cgcaggcgcg cgcactgcat
     3301 gagtgcatcc tgcaagcgct gcagcgcaag aatcgctgcg aggtaagcga gtcccgcctg
     3361 gtgcaccacg cggtacgcgc cggagacatt gcggcggtgc tcgagtttgc gccgcgagct
     3421 gccgagcgct ctgccggagt cggtgcacac cacgaagcga tcgcctacta ccgcaccgcg
     3481 ctcggccacg cggaacgaat gtcgcctgag ctgcgcggca ccttgctcga cagtctcgcc
     3541 tacgagtgct tcctcaccag cgagatcgac gctgcgatcg tggcgcaacg cgacgcgctg
     3601 gctatctggc gctcgagcga aaatgccaag cgggccggag atacgatgcg ttccctgtcg
     3661 cggctgcact gggtgcgcgg gcggggcgcc gaggcacacc gctacgcagc cgaggcggta
     3721 gcggcactcg agccgctcgg gccgagcgac gagctggcga tggccttcag caacatggcg
     3781 caactcgcag tgctcgcctc gcacgatgac gccgcgatcg agtggggtga gcgcgccatc
     3841 gagctcgcct cgcggctcgg gaacgacgag attctggcgc acgctctcaa caacgttggc
     3901 gcggcgcgca cgcgcgcaac aggggatgcc ggctggcgcg agctcgaacg cagcctggag
     3961 ctgtcgttgc gccgcggctt ccaggagcac gtggcacgcg ctcacaccaa tctcgcggcc
     4021 cttgcgatcg gcacgcgcga ctaccgtgcg gctggccctc acctgcgggc cggactcgcg
     4081 ttctgtgccg agcacgagct gtatatggcc gagggctatc tgcgcgcact gcgtgcgcgg
     4141 gtgaatttcg agcaaggggc atggagcgac gcgatcgaag aggcgcaggg cctgctgcgc
     4201 gagcccgggc tgtcaattgc cgcgcgcatc ccggcgttaa ccgtgctcgg ccgcgttctt
     4261 gcgcgacgcg gcaccgaggg cgcactcgcg ccactcgagg aagcgcgcat gctcgccatg
     4321 gcgacgggcg agccgcagcg gatcgtgagc gtcgcggccg cacgcgccga acacgcctgg
     4381 ttacgcggcg atttggcggc cgtcggccgc gagctaggca gcgtcctcga atacgcgttg
     4441 cgagatcggg accact
//
LOCUS       DATDMP010000670         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1548645, whole genome shotgun sequence.
ACCESSION   DATDMP010000670 DATDMP010000000
VERSION     DATDMP010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548645"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VI195_11735"
     CDS             complement(<1..565)
                     /locus_tag="VI195_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEY6825106.1"
                     /translation="MSQAFPIIKRPFRSAKPRTTGLTMVLDKGMGVVALKDWLEIAGE
                     HVDIVKLGWGTSGVMPEAVLREKIRILDERGILVSPGGTFLEIAYAQRRVAPFLQAAR
                     ELGFSCIEVSDGTVTMPHGDKVRLIEQARSRGFTVFSEVGKKFELEDKRYPLEQRIDD
                     LQEELRAGAQKVIIEARESGTFGIFNAE"
     gene            complement(730..1617)
                     /locus_tag="VI195_11740"
     CDS             complement(730..1617)
                     /locus_tag="VI195_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6825107.1"
                     /translation="MSRLFPGFETRRLRTSGATIHLEIGGDGPPLLLLHGYPQTHAMW
                     HKVAPQLAREYTVVCPDLRGYGDSSKPRGVPGHLNYAKRAMALDMAEAMESLGFIAFH
                     VVGHDRGGRVAHRLARDFAKRVRTLTVLDISPTLKMYQSTTLQFAKAYWHWFFLIQEA
                     PLPERLLAGHVPWYILKRLGRGKTGLKYFDRRAIVEYTRAFRDPRTIHATCEDYRAAA
                     TIDLVHDKQDARKKLRMPLLALWGKHGVIQALFDCLADWREVADDVRGRPLDCGHFIA
                     EEKPRELIAELRRFLATRR"
     gene            complement(1614..2495)
                     /locus_tag="VI195_11745"
     CDS             complement(1614..2495)
                     /locus_tag="VI195_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6825108.1"
                     /translation="MKLALKIDVDTLRGTEEGVPRLAELLKKHGAGATFLFSLGPDHT
                     GRAMRRVFRPGFFSKVSRTSVLEHYGVRTLLYGTLLPGPDIGRRCAQVMRAVRDDGFE
                     VGVHCWDHVRWQDFVVRRDAGWTEREMERATRRFHEVFGGPARTWGAAGWQLNRHAAW
                     YEEQHFAYASDTRGSRPFRPLWDGVAIGCPQLPTTLPTLDELIGLHEDVVQQLLSRTG
                     NGDHVFTAHAELEGGRLMPMFEALLAGWKVQGYEIVALEALYRTLDLARLPRCEVVQG
                     TVPGRSGTLAVQQEIRA"
     gene            complement(2521..>2853)
                     /locus_tag="VI195_11750"
     CDS             complement(2521..>2853)
                     /locus_tag="VI195_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825109.1"
                     /translation="ANRNGIASRKIFNIGNPKNNISVRELAESMVKLALEYPEYRAAA
                     RKVRIVKTTADRYYGRGYQDVQNRVPKIDNTMRELAWRPRVGMRDALKRIYDAYRTDV
                     AEARHLVK"
BASE COUNT          452 a          950 c          942 g          509 t
ORIGIN      
        1 cctcggcgtt gaagatgccg aaggtgcccg actcgcgcgc ttcaatgatc actttctgcg
       61 cgccggcacg cagctcctcc tgcaggtcgt cgatgcgctg ctcgagtgga tagcgcttgt
      121 cctcgagctc gaatttcttt cccacctcgg agaacaccgt aaagccgcgg ctgcgcgcct
      181 gctcgatgag gcgcaccttg tcgccatgcg gcatggtgac cgtgccgtcc gagacttcga
      241 tgcaggagaa gccgagctcg cgcgcggcct gcaggaaagg tgcgacgcgc ctttgcgcgt
      301 aagcgatctc gaggaacgtc cctcctggag acaccaggat cccgcgctcg tccagtatgc
      361 ggatcttctc gcgcagcaca gcttccggca tgacaccgga ggttccccag ccgagcttta
      421 cgatgtcgac gtgctcgccg gcgatttcca gccagtcttt cagcgcgacg acgcccatgc
      481 ctttgtcgag caccatggtc aatccggtcg tgcgcggctt ggccgacctg aacggccgct
      541 tgatgatcgg gaacgcctgg ctcaagcgat cactccccct tgggacagag ggccatagtg
      601 cccgcgatga ccatgcccga gccggccggg cacaatgttt tgccgtccgc ggcgctctag
      661 cgcttcacca aatcgtcatc actcgtgtgg cgggggaaaa aacggtgaag cgtttcatct
      721 tgcactcgcc tagcggcgcg tcgcaagaaa gcgcctgagc tcggcgataa gctcgcgcgg
      781 cttttcctcg gcgatgaagt ggccgcagtc gagcggccgg ccgcgcacat cgtcggcgac
      841 ctcgcgccag tccgcgaggc agtcgaacag cgcctggatg acgccgtgct tgccccagag
      901 tgcgagcagc ggcatgcgca gcttcttgcg cgcgtcctgc ttgtcgtgca ccagatcgat
      961 ggtcgcggcg gcgcgatagt cctcgcaggt cgcgtggatg gtgcgcggat cacggaaggc
     1021 gcgcgtgtat tcgacgattg cgcgccggtc gaaatacttc agccccgtct tgccgcgccc
     1081 caagcgcttg aggatgtacc agggcacatg gccggcgagc agccgctcgg gcagtggcgc
     1141 ctcctggatg aggaagaacc agtgccaata ggccttggcg aactgcagcg tggtgctctg
     1201 gtacatctta agcgtcggtg agatgtcgag caccgtgagc gtgcgcacac gcttcgcgaa
     1261 atcgcgcgcc aggcgatggg cgacccggcc gccccggtcg tgcccgacca catggaaggc
     1321 gatgaagccg agcgactcca tcgcttccgc catgtcgagc gccatggcgc gcttcgcata
     1381 gttcaggtgt ccgggcacgc cgcgcggctt ggacgagtcg ccatagccgc gcaggtcggg
     1441 gcagaccact gtgtattcgc gcgcgagctg tggagccacc ttgtgccaca tggcgtgcgt
     1501 ttgcggatag ccgtgcagca ggaggagcgg cggcccgtcg ccgccgatct cgaggtgaat
     1561 cgtcgcgccg ctcgtgcgaa ggcgccgcgt ctcgaagccg ggaaagagcc tgctcatgca
     1621 cggatctcct gttgcaccgc gagcgtcccg gagcggccgg gcaccgttcc ctgcaccacc
     1681 tcgcagcgcg gcaggcgggc gagatcgagc gtgcggtaga gcgcttccag cgcgactatc
     1741 tcgtacccct gcactttcca tcctgcgagt agcgcctcga acatcggcat caacctgccg
     1801 ccttcgagct cggcgtgcgc cgtgaacacg tggtcgccat ttcccgtgcg cgaaagcaac
     1861 tgctgcacca cgtcctcgtg cagcccgatc agctcgtcga gcgtcggcag cgtggtgggt
     1921 agctgcgggc agccgatcgc cacgccgtcc cagagaggcc ggaacggccg gctgccgcgc
     1981 gtatcggaag cgtaggcgaa gtgttgttcc tcgtaccagg ccgcatgccg gttcaactgc
     2041 cagcccgcgg cgccccaggt gcgcgccggc ccgccgaaaa cttcgtgaaa tcgcctcgtc
     2101 gcgcgctcca tctcgcgctc ggtccagccg gcgtcgcgcc gcacgacgaa gtcctgccag
     2161 cgcacatggt cccagcagtg cacgccgacc tcgaagccgt cgtcgcgcac tgcgcgcatc
     2221 acctgtgcgc agcgacggcc gatatccggg ccgggcagca gcgtgccgta tagcagggtt
     2281 cgcacgccgt agtgttcgag caccgacgtg cggctcacct tggagaagaa tccgggcctg
     2341 aagacgcgcc gcatggcgcg gccggtgtgg tcggggccca ggctgaagag aaaagtggcc
     2401 cccgcgccgt gcttcttcag cagctcggcg agccgcggca cgccttcctc ggtgccgcgc
     2461 agagtatcga cgtcgatctt taaggcaagt ttcactgcct ctggcgcggc ttctggcgct
     2521 tcacttcacc agatggcgcg cctcggcgac atcggtgcga taggcgtcgt agatgcgctt
     2581 gagcgcgtcg cgcatgccca cgcgcggccg ccaggcgagc tcgcgcatgg tgttgtcgat
     2641 cttcggcacg cggttctgca cgtcctggta gccgcgcccg taatagcggt cggcggtggt
     2701 cttgacgatg cgcaccttgc gcgcagctgc tcggtactcg gggtattcga gggcgagctt
     2761 caccatgctc tcggccagct cgcgcaccga tatgttgttc ttcggattgc cgatgttgaa
     2821 gatcttgcgc gaggcgatgc cgttgcggtt ggc
//
LOCUS       DATDMP010000671         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_105966, whole genome shotgun sequence.
ACCESSION   DATDMP010000671 DATDMP010000000
VERSION     DATDMP010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105966"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>2010
                     /locus_tag="VI195_11755"
     CDS             22..>2010
                     /locus_tag="VI195_11755"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY6825110.1"
                     /translation="MELTAAWQTEELPRERLTVADEREQILFYLVEILYRVVPAFYEE
                     IAQALEKLYGARGGAARLPSMLHFGTWAGGDMDGNPDVHAKTIRETLARHHQVILHCY
                     FVECHELAQRLSQSESRVAASAELGKRVEEYMRQLPGARAITPARHDRMPYRVFLAQI
                     GERLRCAREGRAHGYENAHQLHDDVALIALSLRANKGVNAGLFYVERLLRRIETFGFH
                     LATLDVRQHASVLHEVIAQGFDDPQWLGRDRGERRNLLAQALEKDRGPVIELGALGRR
                     TLAVFDAIVQARHRYGPEAIGYFIVSGAEGADDMLAALLLARWAEVYDKRTGEIALDI
                     APQFETSAALTTCGNTMQQLLADPLYRRYLEAHARKQCVLIGYSDSNKEIGVCASRFT
                     IHQAQHDLAAVLRNAGEQPVLFHARGGSLARGGARIEAIVKAAPAGAVEGVLRVREQG
                     ATVKQGYGLRPIAMRTLERAFNAVSLTSAALRRGELAPDSSAHLEIAAHLAATSRAAY
                     RALVYGEAQFYRFFEAVTPIDVIERMQIGARSVHRSEEGGLDALLPVPWVFAWAQTRY
                     MLPGWYGAGIGLAAAVDKFGLQRVREACASWLFLRNLLDDIETLLARADLEIAQHYDA
                     LAPQELRRFSAQLREEYDRACTQVLTIRDSRELLDFDPT"
BASE COUNT          315 a          710 c          679 g          306 t
ORIGIN      
        1 agcatctgga gcaacatccg catggagctc accgcggcat ggcagaccga ggagctgccg
       61 cgcgagcggc tcacggttgc cgacgagcgt gagcagattc tgttctacct ggtggagatc
      121 ctctatcggg tggtgcccgc cttctacgag gagatcgccc aggcgctcga gaagctctac
      181 ggcgcccgtg gcggcgcggc acgcctgccc tccatgctgc acttcggcac ctgggccggc
      241 ggcgacatgg acggcaatcc cgatgtgcac gccaagacga tccgcgagac gctcgcgcgc
      301 catcaccagg tgatcctgca ctgctacttc gtcgagtgtc acgagcttgc gcagcggttg
      361 tcgcagagcg agagccgcgt ggccgcctcc gccgagctcg gcaagcgggt cgaggagtac
      421 atgcggcagc tcccgggcgc gcgcgccatc acccccgcgc ggcacgaccg catgccctac
      481 cgcgtgttcc tggcccagat cggcgagcgt ctgcgctgcg cgcgcgaggg gcgtgcgcac
      541 ggttacgaga atgcgcacca gttgcacgac gacgttgcgc tcatcgccct cagcctgcgc
      601 gccaacaagg gtgtgaacgc gggactgttc tatgtcgagc ggctgctacg gcggatcgag
      661 accttcggct tccatctggc gacactcgat gtgcggcagc acgccagcgt gctgcacgag
      721 gtcatcgccc agggcttcga tgatccgcaa tggctcggcc gcgaccgcgg cgagcgccgc
      781 aacctgctgg cgcaggcgct cgagaaggac cgtgggccgg tgatcgagct cggcgcgctc
      841 gggcggcgca cgctcgcggt cttcgatgcc atcgtgcagg cgcgccatcg ctacggcccc
      901 gaagccatcg gctacttcat cgtgagtggc gccgagggag ccgacgacat gctcgcggcg
      961 ctactgctcg cccgctgggc ggaggtctac gacaagcgca ccggcgagat cgccctcgac
     1021 atcgctccgc agttcgagac cagcgcggcg ctcaccacct gcggcaacac catgcagcag
     1081 ctgctcgccg atccgctcta tcggcgctac ctcgaggcgc acgcccgcaa acagtgcgtc
     1141 ctcatcggtt actccgacag caacaaggaa atcggggtat gcgcctcgcg cttcaccatc
     1201 caccaggcgc agcatgatct cgccgccgta ctgcgcaacg ccggcgagca acccgtgctg
     1261 ttccacgccc gcggcgggag cctggcgcgc ggcggagcgc gcatcgaggc catcgtcaag
     1321 gccgcgccgg ccggtgccgt ggagggtgtg ctgcgcgtgc gcgaacaggg cgcgaccgtg
     1381 aagcagggct acggtctgcg gccgatcgcc atgcgcacgc tcgagcgcgc cttcaacgcc
     1441 gtgagcctca cgagtgccgc actgcggcgc ggcgagctgg cccccgacag ctccgcacac
     1501 ctggagatcg ccgcccatct cgctgccacc agccgcgccg cgtaccgggc tctggtttac
     1561 ggcgaggcgc agttctaccg cttcttcgag gcggtcacgc cgatcgatgt gatcgagcgc
     1621 atgcagatcg gcgcgcgctc ggtacatcgc tccgaagaag ggggactgga cgcgctgctg
     1681 cccgtgccgt gggtgttcgc gtgggcgcag acccgctaca tgctgccggg ctggtatggc
     1741 gccggcatcg ggcttgcagc cgccgtcgac aaattcgggc tgcagcgggt acgggaagcg
     1801 tgtgcgtcct ggttgttcct gcgcaacctg ctcgatgaca tcgagacgct gctggcgcgc
     1861 gccgacctgg agatcgccca gcactacgac gcgctcgccc cgcaggagct gcggcgcttc
     1921 tccgcgcagc tgcgcgagga atacgaccgc gcatgcaccc aggtgctcac gattcgcgac
     1981 agccgcgagc tgctcgattt cgacccgacc
//
LOCUS       DATDMP010000672         2059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1439218, whole genome shotgun sequence.
ACCESSION   DATDMP010000672 DATDMP010000000
VERSION     DATDMP010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439218"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VI195_11760"
     CDS             complement(<1..379)
                     /locus_tag="VI195_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase small subunit"
                     /protein_id="HEY6825111.1"
                     /translation="MADKNLQFLDIPRTEPGKSSVEARLRDFREIYSPYDAAGAASQA
                     GRCLACGNPFCEWKCPVHNYIPNWLKLIEEGNLLEAAELSHRTNSLPEVCGRICPQDR
                     LCEGACTLNDGLGAVTIGAIEKYI"
     gene            complement(396..>2059)
                     /locus_tag="VI195_11765"
     CDS             complement(396..>2059)
                     /locus_tag="VI195_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY6825112.1"
                     /translation="QIKIAQGAKPGEGGQLPGHKVNEMIATLRFARPGVGLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPAALVSVKLVAEPGVGTVAAGVAKAYADLITISGYDGGT
                     GASPLSSIKYAGTPWELGLAETHQTLRVNELRHRVRLQTDGGLKTGLDVVKAAIIGAE
                     SFGFGTAPIVALGCKYLRICHLNNCATGVATQHAVLRSKYFTGLPEMVINYFRFVAEE
                     VREILASLGVRSLQEIIGRTEYLEVLPGATPKQRKLDLTPILSDAGLASDAPRCCVTE
                     RNAPFDRGELAERMVRDMLPAIESKAGGEWHYRLSNFNRSIGARVSGEIARRWGNYGM
                     EGAPLTVRCTGSAGQSFGVWNAGGLHLYLEGDANDYVAKGMTAGRIVLRHPRGARFAA
                     RDTVIMGNTCLYGATGGELYAAGLAGERFAVRNSGAVAVIEGAGDHCCEYMTGGAVCV
                     LGRTGVNFGAGFTGGFAYVLDLERNFVDRYNHELIDISRIHPEAMQSHVQHLEGLLAR
                     HVEETGSTWAGEVSADLRGFLPKFWVVKPKAASIDSLIENLRRAA"
BASE COUNT          312 a          696 c          706 g          345 t
ORIGIN      
        1 tgatgtattt ctcgatcgcg ccgatggtga ccgcacccaa gccgtcgttg agggtgcagg
       61 caccctcgca caggcgatcc tgcgggcaga tgcgtccgca cacctcgggg agcgagttgg
      121 tgcggtggga aagctcggcc gcctccagca gattcccctc ttcgatgagc ttcagccagt
      181 tgggaatgta gttgtggacc gggcacttcc actcgcagaa ggggttgccg caggcgaggc
      241 agcgaccggc ctggctggcc gcgcccgcgg cgtcgtaggg cgagtagatc tcgcggaaat
      301 cccgcagccg cgcctcgacg ctgctctttc ccggctcggt gcgcgggatg tcgaggaact
      361 gcaggttctt gtcggccatg tccgggactg acgcctcagg ccgcgcgccg cagattctcg
      421 atgagcgagt cgatggaggc ggccttgggt ttgaccaccc agaacttggg caggaagcca
      481 cgcaggtccg cgctcacctc cccggcccag gtgctgccgg tctcctcgac gtgccgcgcg
      541 agcagcccct cgaggtgctg cacgtgcgat tgcatcgcct cgggatgaat gcggctgatg
      601 tcgatcagct cgtggttgta gcgatcgacg aagttgcgct cgaggtcgag cacgtaggcg
      661 aagccgccgg tgaagccggc gccgaagttg acgccggtac gtccgagcac gcacaccgct
      721 ccgcccgtca tgtactcgca gcagtggtcc ccggcgcctt cgatcacggc caccgccccg
      781 gaattgcgta ccgcgaagcg ctcgccggcg agccccgccg cgtacagctc accgccggtc
      841 gcgccgtaca ggcaggtgtt gcccatgatg accgtgtcac gggccgcgaa gcgcgcaccg
      901 cgcgggtgac gcagcacgat gcgtccggcc gtcatgccct tggcgacgta gtcgttggca
      961 tcgccctcga gatacaggtg cagtccgccg gcattccaca ccccgaagct ctgcccggcg
     1021 ctgccggtgc agcgcaccgt gagaggcgcg ccctccatgc cgtagttgcc ccagcgccgc
     1081 gcgatctcac cggagacgcg tgcgccgatc gagcggttga aattgctgag ccggtagtgc
     1141 cactcgccgc cggctttgct ctcgatggcc ggcagcatgt cgcgcaccat gcgctcggcg
     1201 agctcgccgc ggtcgaaggg cgcgttgcgc tcggtgacgc agcagcgcgg cgcgtcgctc
     1261 gccaggcccg cgtccgacag gatcggcgtg agatcgagct tgcgctgctt cggcgtagcc
     1321 ccgggcagca cctcgaggta ctcggtgcgg ccgatgatct cctgcagaga acgcacgccg
     1381 aggctggcga gaatctcgcg tacctcctcg gccacgaacc ggaaatagtt gatcaccatc
     1441 tcggggagcc cggtgaagta cttcgagcgc agcaccgcgt gctgggtcgc caccccggtg
     1501 gcgcagttgt tgagatggca gatccgcagg tacttgcacc ccagagccac tatcggcgcg
     1561 gtgccgaagc cgaagctctc cgcaccgatg atggcggcct tcaccacgtc cagcccggtc
     1621 ttcaggcccc cgtcggtctg cagccgcacc cgatggcgca gctcgttcac gcgcagcgtc
     1681 tggtgcgtct cggcgagccc gagctcccac ggtgtgccgg cgtacttgat ggaggagagc
     1741 ggactcgccc cggtgccgcc gtcgtagccg gagatggtga tgagatcggc ataggccttg
     1801 gcgactccgg ccgccacggt gccgaccccg ggctcggcga ccagcttcac cgagaccaga
     1861 gcggcggggt tcacctgctt gagatcgaag atcagctgcg ccaggtcctc gatggagtag
     1921 atgtcgtggt gcggcggggg tgagatgagt cccacgccgg gccgggcgaa gcgcagcgtg
     1981 gcgatcatct cgttgacctt gtggccgggc agctgtccgc cttcgccggg cttcgctccc
     2041 tgggcgatct tgatctgca
//
LOCUS       DATDMP010000673         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_216563, whole genome shotgun sequence.
ACCESSION   DATDMP010000673 DATDMP010000000
VERSION     DATDMP010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216563"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1361
                     /locus_tag="VI195_11770"
     CDS             159..1361
                     /locus_tag="VI195_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6825113.1"
                     /translation="MTVSRWSSALGAALLAASPYAAQAAGEPVVIGDIDDMSGVYADV
                     EGAGGVEAINMAIADFGGTVLGRKITVLSADHQNKPDIGASKFREWADQQGLNLLLGG
                     SNTGVNVAMSKVAGAKKVPLIVIGAGGASLTNEDCTAYSVHYVYDTTALANGTATAMI
                     NAGGKTWFYLTADYAFGTQLQSAAAKVVETRGGKNLGAVRVPLGAADFSSFLLQAQAS
                     GAQVLGLANAGTDFSNSLKAANEFGITRTMKPAALLAFLTDIHSLGLKTAQGLYLTTG
                     WYWDLNDETRAFAKRFFAKMNRQPTMDHAGVYSATLTYLKAVKAAGSTDPDRVMAQLK
                     KDKINDMFAKGGYIRADGAMIHEMYVMQVKTPQESKYSWDYYKLIRKMSGEDAFGPIT
                     GLCPLARR"
     gene            1387..2271
                     /locus_tag="VI195_11775"
     CDS             1387..2271
                     /locus_tag="VI195_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY6825114.1"
                     /translation="MSTVLGIPVPVILGQLTLGLVNGSFYALLSLGLAVIFGLMGVVN
                     FAHGAFYTLGAFAALLGLQYLGINYWLALLLAPLAVGLLGVLVERLLLRHLYGLDPLY
                     GLLLTFGLALIVEGSLHQVFGASGQSYPVPESLAGALNLRFMFLPLYRAWVILASVLV
                     CFGTWFIIERTRLGATLRAATENAPLLQAFGVNVPLLVAATYGGGVALAALAGVLASP
                     VVLVNSHMGNELVIVVFAVVVIGGMGSILGSIVSGLLLGLIEGLTKVVYPEASNIVVF
                     LIMALVLILRPAGLFGRE"
BASE COUNT          368 a          740 c          748 g          454 t
ORIGIN      
        1 gcagaacttc cggttcgccg cgccgctcgc cgaccgcttc tacatcatgg agcgcggacg
       61 catcgtggcg ggcttcgcgg cctgcgagct tcaagccaaa atggcgctgt tacaccagtt
      121 tctcggcgtt tgaccccttc ctactcagga taagaccaat gacagtgagc aggtggagca
      181 gcgcgctcgg tgccgccttg cttgcggcga gtccgtatgc ggcgcaggcg gcgggcgagc
      241 cggtggtgat cggcgacatc gacgatatgt ccggggtgta cgcggacgtc gagggcgcgg
      301 gtggcgtcga agctatcaac atggcgatcg ccgacttcgg tggcacggtg ctcggcagga
      361 agatcaccgt gctgagcgcc gatcaccaga acaagcccga catcggcgcc tcgaagttcc
      421 gtgagtgggc cgatcagcag ggtctcaacc tgttgctcgg cggctccaac accggagtca
      481 acgtcgccat gtcgaaggtc gcgggtgcca agaaggtgcc cctcatcgtg atcggcgcgg
      541 gaggtgcctc gctcaccaac gaggactgca ccgcctattc ggtgcattac gtgtacgaca
      601 ctacggcgct cgccaacggc accgcgaccg ccatgatcaa cgctggcggg aagacctggt
      661 tctatctgac tgcggactat gccttcggca cgcagctgca gagcgcagcc gcgaaggtcg
      721 tggagactcg tggcggcaag aatttgggcg cagtgcgcgt gccgctcggg gcggcggatt
      781 tctcctcgtt cctgctgcag gcccaggcct ccggcgcaca ggtgctcggg ctcgccaatg
      841 ccggcaccga cttcagcaac tcgctcaaag ccgccaacga gttcggcatc acccgcacca
      901 tgaagcccgc ggcgctgctc gccttcctca cggacatcca tagtctcggc ctcaagacgg
      961 cgcagggtct gtacctgacc accggctggt actgggacct caacgacgag acgcgcgcct
     1021 tcgccaagcg gttcttcgca aagatgaacc gtcagccgac catggaccac gcgggcgtat
     1081 attcggcgac gctcacctat ctgaaggcgg tgaaggccgc cggcagtacc gatcccgata
     1141 gggtcatggc gcagctgaag aaggacaaga tcaacgacat gtttgccaag ggcggctaca
     1201 tccgcgccga tggcgccatg atccacgaga tgtatgtcat gcaggtgaag acgccgcagg
     1261 agtcgaagta ttcctgggac tactacaagc tgatccggaa gatgagcggg gaggacgctt
     1321 tcggaccgat taccggcctg tgcccgctcg cccgcaggta gtcgtgcgca cccagcgcgc
     1381 cgcgccgtga gcacggtcct cggcatcccc gtcccggtga tactcgggca gctgacgctc
     1441 ggcctggtga acggctcgtt ctatgccctg ctgagtctcg ggctggcggt catcttcggg
     1501 ctgatgggcg tcgtgaactt cgctcacggc gccttctaca cgctcggcgc ttttgctgct
     1561 ctgcttggac tgcaatacct cggaatcaac tactggctgg cgctgctgct cgcgccgctc
     1621 gccgtagggc tgctcggggt gctggtagag cggctgttgc tgcggcacct ctatggtctc
     1681 gatccgctct acggattgct gctcactttc ggcctggcgc tcatcgtcga gggatcgctc
     1741 caccaggtgt tcggcgcgag cggccagtcc tatccggtgc cggagtcgct cgccggagcg
     1801 ctcaacctgc gtttcatgtt tctgccgctg taccgcgcct gggtgattct cgcctcggta
     1861 ctggtgtgct tcggcacctg gttcatcatc gagcgcacgc ggctcggcgc gaccctgcgc
     1921 gccgccaccg agaatgctcc actgctgcag gccttcggcg tcaatgttcc gctgctggtc
     1981 gctgccacct acggcggagg ggtggcgctc gcggcgcttg cgggcgtgct cgcctccccg
     2041 gtggtgctgg tgaattcgca catggggaac gagctcgtca tcgtcgtatt cgcggtagtc
     2101 gtgatcggtg ggatgggctc gattctcggc tcgatcgtga gcggcctgct gctcggcctg
     2161 atcgaaggcc tcaccaaggt cgtttatccg gaagcctcca acatcgtggt gttcctcatc
     2221 atggcgctgg tactcatcct gcggcctgcc ggcctcttcg gccgggagta gcgcgatgcg
     2281 cgtgttctat gtgctcatgg gtctactcgc
//
LOCUS       DATDMP010000674         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_439702, whole genome shotgun sequence.
ACCESSION   DATDMP010000674 DATDMP010000000
VERSION     DATDMP010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439702"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VI195_11780"
     CDS             complement(<1..278)
                     /locus_tag="VI195_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY6825115.1"
                     /translation="MTAHGSRSFQATPNLRHLVLFDSVVRRGSVSAAARGAHLSQPAV
                     TQAIAHIESALGARLLQRSYAGLSLTGLGNAAAHRVERALQMLREALSA"
     gene            377..1066
                     /locus_tag="VI195_11785"
     CDS             377..1066
                     /locus_tag="VI195_11785"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEY6825116.1"
                     /translation="MNVVYTNIRRAEPEVVKGLARYGVATIHEAQGRTGLLGPALRPV
                     YTGARIAGPAVTVSLPPADNWMIHVAVEQCQAGDILVVAPTSASDAGYFGELLATSLA
                     ARGVRGLIIDGGVRDVHELKRMGFPAWSRAVHAQGTVKETLGSVNVPVVCAGGLVDPG
                     DVVIADDDGVCIVPRLEAAAVLKASEAREMKEAATRQRLQKGELGLDIYDMREKLAKR
                     GLIYRAQALPE"
     gene            1072..2163
                     /locus_tag="VI195_11790"
     CDS             1072..2163
                     /locus_tag="VI195_11790"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEY6825117.1"
                     /translation="MLRPIPSTLMRGGTSKALYFHVKDLPQSVRARDRVLLAAMGSPD
                     PRQIDGVGGAHPLTSKVAIIGPATRADADVDYLFLQVVVDKAEVSDSQNCGNILAGVG
                     PWAIEQGLVRITSASTPVRIHMLNTRSVAVAHVPTPHGKVEYEGDARIDGVPGSAAAI
                     SIDFLEVAGSSCGKLFPTGSNVDRIEGIEVTCIDNGMPVVLMRAADLGKRGDEAPQEL
                     EADAALTGRIERLRLAVGPRMNLGDVARKTVPKMCLVSAARHGGTLHTRTFIPHRVHE
                     AIGVLGAVSVATACVAPGSVAAQVAGLSPRSGLQRLEIEHPTGFFTVEMEVTLDGATV
                     TVQRSALLRTARKLMQGEVFVPGSAWSDS"
BASE COUNT          363 a          757 c          784 g          369 t
ORIGIN      
        1 gcggagagcg cctcccgcaa catctgcagg gcccgctcga cccggtgcgc ggcggcattg
       61 ccgaggccag tgagcgagag ccctgcgtaa ctgcgttgca gcaggcgtgc gcccagcgcc
      121 gattcgatat gagcgatggc ctgagtcact gccggctggc tcaggtgggc cccgcgggcc
      181 gccgcgctca cgctgccgcg gcgcacgacc gaatcgaaca gcacgagatg tcgcagattc
      241 ggcgtcgcct ggaagcttcg cgatccgtgg gccgtcatgt tcacaatttc ttatacccag
      301 accccaattg caattacccc gcctggcggc tgcggcggga caatcgatcc ctgccaagga
      361 ctcccagggt gccgccatga atgtcgtgta caccaatatt cgccgcgccg aacccgaggt
      421 ggtcaagggc ctggcgcgct acggcgtggc aaccattcac gaggcgcagg gccggacggg
      481 tctgctgggc ccggcgctgc ggccggttta taccggcgcg cgcatcgccg gtcccgcggt
      541 cacggtgagt ctcccgcccg cggataactg gatgatccac gttgcggtcg agcagtgcca
      601 ggcaggggac attctggtgg ttgcaccgac ttccgcttcg gacgccggct acttcgggga
      661 gttgctggcc acatcgctcg ccgcacgcgg cgttcgcggt ctgatcatcg atggcggcgt
      721 ccgcgacgtg cacgagctca agcgcatggg cttccccgcg tggtcaaggg ccgtacacgc
      781 acagggaacc gtcaaggaga cgctcggctc ggtgaacgtt ccggtcgttt gcgccggagg
      841 gcttgtcgat cccggtgacg tggtgatcgc ggacgatgac ggcgtgtgca tcgtgccgcg
      901 cctcgaggct gcggcggtcc tcaaagccag cgaggcgcgt gagatgaagg aagccgccac
      961 gcgccagcgt ctgcagaaag gcgagctcgg tctcgacatc tacgacatgc gggagaagtt
     1021 ggcgaagcgc ggcctcatct accgcgcgca agccctgccg gagtagcgcc gatgctgcgc
     1081 cccattccca gcacgctcat gcgcggcggg acctccaagg cactctattt ccacgtgaaa
     1141 gacctgccgc agtcggtgcg ggcgcgagac cgcgtgctgc tcgccgccat gggctcgccc
     1201 gatccgcgcc agatcgatgg cgtgggcggc gcccatccac ttaccagcaa ggtcgccatc
     1261 atcggccccg ccacgcgcgc ggacgcggac gtggactatc tgttcctgca ggtggtggtg
     1321 gacaaggcgg aggtgagcga ctcgcagaac tgcggcaaca tcctcgccgg cgtgggtcct
     1381 tgggccatcg agcagggcct cgtgcgcatc accagcgcat ccacgccggt gcgcatccac
     1441 atgctcaaca cgcgcagcgt cgccgtggcg cacgtgccga cgccgcacgg taaggtggaa
     1501 tacgagggtg atgcccgcat cgatggcgta cccgggagcg cggcggcgat ctccatcgac
     1561 ttcctggagg tcgccgggtc ctcgtgcggc aagctgtttc ccaccggctc caacgtcgac
     1621 cgcatcgagg gaatcgaggt gacctgcatc gacaacggca tgccggtggt gctgatgcgc
     1681 gccgccgacc tcggcaagcg cggcgatgag gcgccccagg agctcgaggc cgatgcggcg
     1741 ctcaccggcc gcatcgagcg cctgcggctc gcagtgggcc cgcggatgaa tctcggcgat
     1801 gtggccagga agacggtacc caagatgtgc ctcgtctcgg ccgcgcgtca cggcggcact
     1861 ctccacacgc gcaccttcat accgcaccgc gtgcacgagg cgatcggggt cctcggtgcc
     1921 gtcagcgttg ccaccgcctg tgtggctccc ggctcggtag ccgcacaggt ggcgggcctg
     1981 agtccgcgct caggtctgca gcgcctggag atcgagcacc cgacgggatt cttcacggtg
     2041 gagatggaag tgacgctgga cggtgcgacc gtcaccgtcc agcgttccgc cttgttgcgc
     2101 actgcgcgca agctcatgca gggcgaggta ttcgtgccgg gaagtgcgtg gagcgattca
     2161 tgacgcagcg gcgctccatc gccctcatcg ggcttgggga agtcggccgg gtgctggcga
     2221 ccgacctcaa ccgcgatagc agtgtcgatc tgcatgcgtg ggatcggctc ttt
//
LOCUS       DATDMP010000675         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_328921, whole genome shotgun sequence.
ACCESSION   DATDMP010000675 DATDMP010000000
VERSION     DATDMP010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328921"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /gene="tig"
                     /locus_tag="VI195_11795"
     CDS             complement(<1..1359)
                     /gene="tig"
                     /locus_tag="VI195_11795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY6825118.1"
                     /translation="MEVSLSETGGLSRRLEVAVPATEVAREVQTRLKRLSRTARLKGF
                     RPGKAPLAVITKQFGEQVRAEVLSDLMRSSFAQAVSQEKLRPAGGPRIEPLALDPESD
                     LKYAAHFEVLPEININPAESLALERPTAEVTEADIDAMIENMRQQRPRFTPVERAAHL
                     TDRVRLDYQARMGGKPIEGGDLSDVHVVIGSGQAMPELEEGLKGVQAGEQRTLNVVFP
                     AAHPNKKLAGQSAELHLSIKAVEEQSLPAVDQEFFQTYGVEQGGLTEMRAEVRNSMEH
                     ELAEVVRGRMRVQALDALYRTNPIEVPQALLQEQVQQLQIETARRLGIRDASQLPRPE
                     TFLEPARRRVALGLLVAHIVQSQGLKADRERVLARLNALVEAYPNPDEARRAYLQSAE
                     AMRQIESAALEDQVVDWVVERARVSERPMSFAELTGFGRSAPAQDHDTPHDHGHAPAE
                     GSE"
     gene            complement(1430..1514)
                     /locus_tag="VI195_11800"
     tRNA            complement(1430..1514)
                     /locus_tag="VI195_11800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Leu,seq:tag)
     gene            1887..>2408
                     /locus_tag="VI195_11805"
     CDS             1887..>2408
                     /locus_tag="VI195_11805"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY6825119.1"
                     /translation="MSAADISPHLPPLASVAAALRLTTEHLAAELADPKPAAPEWSEF
                     EWGAARAVAAMHGISALLAEKLLWRGPQEWSGFLSRQREHIARRQLRLQGLLGAVADQ
                     CLQAGIPVQALKGSALHGAGLYGPAQRPMADLDLLTAPRHIAQLTKILEGLGLRESRR
                     TFKHRIFEPRDATR"
BASE COUNT          392 a          813 c          759 g          444 t
ORIGIN      
        1 ctcgctcccc tcagcgggcg cgtgcccgtg atcgtggggc gtgtcgtggt cctgtgccgg
       61 cgcagatctg ccaaaccccg tgagctccgc gaaactcatc ggccgctcgc taacgcgggc
      121 gcgctcgacc acccagtcga ccacctggtc ctcgagcgcg gcggactcga tctgacgcat
      181 cgcctcggcg ctctgcagat acgcacgccg cgcctcgtcg ggatttggat aggcctctac
      241 cagcgcatta agtcgcgcca gcacgcgctc gcgatccgcc ttcagaccct ggctctgcac
      301 gatatgcgct accaggagtc cgagcgccac gcggcgccgc gctggctcga ggaacgtctc
      361 gggacgagga agctggctgg catcgcggat tcccaggcgc cgcgccgtct cgatctgcag
      421 ctgctgcacc tgttcctgga ggagcgcctg cggaacctcg atggggttgg tgcggtagag
      481 cgcatcgagc gcttgtacac gcatacggcc gcgaacgacc tcggccagct catgttccat
      541 gctgttgcga acctcggcac gcatctcggt caggccacct tgttcgacac cgtacgtctg
      601 aaagaattcc tggtcgactg ccggaagaga ttgttcctcc accgccttaa tggagagatg
      661 cagctccgct gactgaccgg cgagcttctt gttcggatgc gcggccggga acaccacgtt
      721 gagcgtgcgc tgctccccgg cctgtactcc cttgaggccc tcctcgagct cgggcatcgc
      781 ctgcccgctg ccgatcacca cgtgcacgtc gctgagatcg cccccctcga tcggcttgcc
      841 acccatgcgt gcctgataat cgagccttac ccggtcggtc aggtgggcgg cgcgctcgac
      901 cggggtgaac cgcgggcgct gctggcgcat gttctcgatc atcgcgtcga tgtccgcctc
      961 ggtcacctcc gcggtcggcc gctcgagagc gagcgattcc gctggattga tgttgatctc
     1021 gggcagcacc tcgaaatgcg ctgcgtactt gaggtcactc tccggatcca aggcgagcgg
     1081 ctcgatgcgc gggccgccgg ccgggcgaag cttctcctgg ctcacggctt gagcaaagga
     1141 gctgcgcatg agatcgctca acacctccgc ccgcacctgc tccccgaact gcttggtgat
     1201 taccgcgagc ggcgccttgc cgggacgaaa acccttgaga cgcgcggtgc gcgagagccg
     1261 cttgagtcgc gtctgtacct ccctggcgac ctcggtcgcc gggacggcga cttcgagtcg
     1321 ccgcgacagc ccacccgttt cagacaggga cacttccatc aattcaattc cttagccctg
     1381 aagcctcgcg caccggagcc agtattcgac agagaaccgc acagagaact ggtgcgaaag
     1441 gagagactcg aactctcacg ggtttcccca ctggattcta agtccagcgc gtctaccagt
     1501 tccgccactt tcgccggtgc cgtgcgcacc ggacagatcg ggtgcgccgt gtctcggtcg
     1561 tgcgtcgggg catttagaaa attatagccg atcgcctgtg cgccccaacg cgcgggccgg
     1621 aagatcaccg attcccgcgc gtatctttgc cgtgttttgt aaaccacgtt aaatcataga
     1681 cttatggtga aagcggtgcc gtcaggatct ggtgcccggc gctgaggcgg ctgaaccggc
     1741 ggcaccactg atcgacaccc actattgcgc cggtgcacgc gccgtttgcg agcgaggttg
     1801 gaatgcgctc caccgatgga ctggagtagg ctcggccgct gaagcggtct cgatggctgc
     1861 cgggcgtgag cgggtgaaca gaccctatga gtgctgcaga catctcaccg catctgccgc
     1921 cgctggcctc ggttgctgcc gcgcttcgcc tcaccaccga gcacctcgcc gcggagcttg
     1981 cggatccgaa gcctgccgcg ccggagtggt cggagttcga atggggcgcc gctcgcgccg
     2041 tcgctgccat gcacggaatc tccgccctgc tcgccgagaa gctgttgtgg cgcggtccgc
     2101 aggagtggtc ggggttcctg agcaggcagc gcgagcatat cgcgcgccgg cagctgcgcc
     2161 tgcaagggct attgggcgcc gtcgcagatc agtgtctaca ggcaggcatc cctgtacagg
     2221 cgctgaaggg ctcggccctg cacggcgcag gtctctatgg gcccgcacag cggcccatgg
     2281 cggacctgga tcttctgacc gcgccgcggc acatcgcgca gcttacgaag atactcgaag
     2341 gcctcggact acgtgagtcg cgtcgtacct tcaaacatcg gatttttgag ccgcgcgacg
     2401 ccacacga
//
LOCUS       DATDMP010000676         3482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1661375, whole genome shotgun sequence.
ACCESSION   DATDMP010000676 DATDMP010000000
VERSION     DATDMP010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661375"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1320)
                     /locus_tag="VI195_11810"
     CDS             complement(187..1320)
                     /locus_tag="VI195_11810"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY6825120.1"
                     /translation="MKRREFSQLLAGAALLGVAHSAAHAAAAVANEPAGVSDAARRLY
                     ASALILDCNSGPPLPERLPLPQADLELVRASGIDVVKLSLGGINSDFAHTVAEIAQVQ
                     QLIELHPDYFAAVRGAADMVRAQREHKLGIILSFESVEMLAGQLSSLELFRNLGVRVM
                     QLSYNRTSAFAAGVMEPHAGGLTALGHEALREMNRLGIAIDISHANPSTTADVLALSS
                     QPPVMTHAGCAAIHPHPRNKTDEQLRALAVRGGVVGIYDLPYLTASPRQPSVDDYMAH
                     LTHALEVAGEEHVGIGSDVGIAPFDTSPAGMTEFSRELKERRAAGLSAPEEDRPTYVV
                     GLNLPRRMEIIADRLLRRGYSAAVTEKVLGANFARVFTQTWGG"
     gene            complement(1317..1781)
                     /locus_tag="VI195_11815"
     CDS             complement(1317..1781)
                     /locus_tag="VI195_11815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6825121.1"
                     /translation="MSQSSRGWVAAVTLAAVLVSPASGAANRSYIEHSVPPGSSPLPF
                     SDAVRSGDTLYVAGHIGIDTRTGNAPSDPATEARLVMEAVKRTVESAGLTMDDLVSVT
                     IYCTDLQLYDMFNAVYRSYFHGHYPARAFIGTDKLLRGGHFEVQGLALKSTP"
     gene            1948..2091
                     /locus_tag="VI195_11820"
     CDS             1948..2091
                     /locus_tag="VI195_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825122.1"
                     /translation="MSALGLRAIMLLLAVVSGFPARSPAAAEVPGAGRAVAATTAALE
                     RAT"
     gene            2095..2724
                     /locus_tag="VI195_11825"
     CDS             2095..2724
                     /locus_tag="VI195_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEY6825123.1"
                     /translation="MADALAPGRRPVWEHYADPALTYVTEDNEVKSRVQLLADVKPLP
                     PGSSGWIVVQEFRCTDFGSFAVTTYVMDEHETIEGHELHARYRGSDTWRATADGWRLS
                     AAQVYAIPLDPPRGEAASALADYQGAYALSDSTRQTIRLDGDHLVAERPGRSAQLLLP
                     ESGDVFFTPGRPRTRRIFTRAPDGQVRGFADRREGTDLVWTRLAPDAAR"
     gene            complement(2721..3269)
                     /locus_tag="VI195_11830"
     CDS             complement(2721..3269)
                     /locus_tag="VI195_11830"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY6825124.1"
                     /translation="MRYWHEIDSPIGPLLLLGSAQALLRVHFQAGPHPLRPGEDWRRA
                     AAPFAQAQAQLMEYFGGTRRSFQLSLAPAGTPFQLAVWRALSAIPYGETVSYGEIARR
                     LGLEAGARAVGLANGANPLPIIVPCHRVIGADGTLTGFGGGLHIKRALLALEGATCVM
                     DLFAAEERALFKPWRASGVPRP"
     gene            complement(3266..>3482)
                     /locus_tag="VI195_11835"
     CDS             complement(3266..>3482)
                     /locus_tag="VI195_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825125.1"
                     /translation="DLVLRRMAARATRLLSVRELEERARAWRPWRGYAVMHLWRAATA
                     ATIEVPSTGGRAITARRAPMARAGSVA"
BASE COUNT          568 a         1177 c         1194 g          543 t
ORIGIN      
        1 agctcgacgc cagcggctca ccccccggcg ccggcgcact gttcggtctg gcgttcgacg
       61 cggcggctgg cgtgtatttc gtcgacgacg ccaccaatac gctcaacctg ttgcatcact
      121 gagcgcagca gggtgacgag cgctcccgct ccggcggacc ccgcggtccc gccggagcgc
      181 gatttctcag ccgccccagg tctgcgtgaa gacgcgtgcg aagttcgcgc ccagcacctt
      241 ctcggtgacc gcggcggaat agccccgccg caacagccgg tcagcgatga tctccatgcg
      301 tcgcgggaga ttgagcccga ccacgtaggt cggacggtct tcctcgggag cggatagacc
      361 cgcggcgcgc cgctccttga gctcacgcga aaattccgtc atgccagcgg gcgaggtgtc
      421 gaagggcgca atgccgacat cgctgccaat cccgacgtgc tcctcgcccg ccacctcgag
      481 cgcgtgtgtg aggtgcgcca tgtagtcatc cacgctcggc tggcgcggcg aggcggtgag
      541 ataaggcaga tcgtagatgc cgaccacgcc gccgcgcacg gcgagtgcac gcaactgctc
      601 gtcggtcttg ttgcgcggat gaggatgtat ggcggcacag ccggcgtggg tcatgaccgg
      661 cggctgggat gagagcgcga gcacatcagc cgtggtcgaa ggattggcgt gactgatgtc
      721 gatcgcgatg ccaaggcgat tcatctcgcg tagcgcctcg tgcccgagcg cggtcagccc
      781 gccggcatgc ggctccatta ccccggcagc gaaggcggag gtgcgattgt aggacagctg
      841 catgacgcgc acaccgaggt tgcggaacag ctcgagcgag ctcagctgtc cagcgagcat
      901 ctccacgctc tcgaaggaga ggatgatccc gagcttgtgc tcgcgctgtg cccgcaccat
      961 atccgcggcg ccacgcaccg cggcgaagta atcggggtgc agctcgatga gttgctgcac
     1021 ctgcgcgatc tccgccacgg tgtgcgcaaa atcgctgttg atcccaccga gtgagagctt
     1081 gacgacgtcg atgccgctgg cgcgcaccag ctcgaggtct gcctgcggca gcggcagccg
     1141 ctccggcagc ggtggcccgg agttgcagtc gagaatgagc gcgctcgcat acaggcggcg
     1201 agcggcatcg cttacgcccg ccggctcatt ggcgaccgcg gctgccgcat gtgctgccga
     1261 gtgagcgaca ccgaggagtg ccgctccggc aagcagctgc gagaactcgc gccgcttcac
     1321 ggtgtgctct ttagcgccag cccctgcacc tcgaagtgtc ctccccgcag caacttgtcg
     1381 gtgccgatga acgcccgcgc cggatagtgc ccatgaaaat agctgcggta gaccgcattg
     1441 aacatgtcgt acagctgcag gtcggtgcag tagatcgtca cggacacgag gtcgtccatc
     1501 gtgagtccgg cggattccac ggtgcgtttc accgcctcca tgaccaggcg tgcctcggtc
     1561 gccggatcgc tgggagcgtt gccggtgcgg gtgtcgatgc cgatgtggcc ggcgacgtat
     1621 agggtgtcgc ccgagcgcac cgcgtcgctg aagggcagtg gcgagctgcc gggcggcacg
     1681 ctgtgctcga tgtagctgcg attggctgcg ccgctagcgg gcgataccaa cacagcggcg
     1741 agcgtcactg cggcgaccca ccctctggat gactgactca tggtgtctgc tccggtaaag
     1801 gggcgccggc aacgcgccag cggctccggg cggcagatgc tatcagcact cgagccgggc
     1861 gcggcccgca cttgagtgat gcagccggtt tttctagcct gcggatcact tcatggcgcg
     1921 gtgcggccgt caggccggga gggccgaatg agcgcattgg gattgcgggc gatcatgctg
     1981 ctgttggcgg tggtgagcgg cttccccgcc cgcagccctg cggcagccga agtacctggg
     2041 gcaggtaggg ccgtcgcggc caccacggca gcgctcgagc gcgcgactta ggctctggcc
     2101 gacgcgctcg cacccgggcg gcgtcccgta tgggagcact acgcggatcc ggcgctgacc
     2161 tacgttactg aggacaacga agtgaagagc cgcgtgcagc tgctcgctga cgtgaagccg
     2221 ctgccaccgg gctcgagcgg gtggatcgtg gtccaggagt tccgctgcac cgatttcggc
     2281 agcttcgcgg tgaccaccta tgtgatggac gagcacgaga ctatcgaggg acatgagctg
     2341 cacgcgcgct accgcggcag cgatacctgg cgcgcgactg ccgatggctg gcggctgagc
     2401 gctgcgcagg tctacgcgat accactcgat ccaccacggg gcgaggcggc gagcgcgctt
     2461 gcggactacc agggcgccta tgccctgagt gacagcacgc ggcagacgat ccgtctcgat
     2521 ggcgatcatc tcgtggcgga gcgtcccggc cgatccgcgc aactgctgct gcctgagagc
     2581 ggcgatgtgt tcttcacccc cggccggccg cgcacacgcc ggatattcac gcgcgcgccc
     2641 gacgggcagg tgcgcggctt cgccgaccgg cgcgagggca ccgatctcgt gtggacacgc
     2701 ctcgcaccgg atgcggcgcg ctagggacgc ggtacgccgc tcgcgcgcca aggcttgaat
     2761 agagcgcgct cctcggccgc aaacagatcc atcacgcagg tcgcgccttc gagcgcgagc
     2821 agcgcacgct tgatgtgcag tcctccacca aagcccgtca gcgtcccatc ggcgccgatg
     2881 acacgatgac agggcacgat gatcggcagc gggtttgcgc cattcgccag tcctaccgcg
     2941 cgggcgccag cttcgagccc gagccggcgg gcgatctcgc catatgacac cgtctcgcca
     3001 taggggatcg cggacagtgc acgccacacc gcgagctgaa acggcgtgcc cgcgggcgcg
     3061 agcgaaagct ggaagctgcg tctggtaccg ccgaaatact ccatcagctg ggcctgtgcc
     3121 tgcgcgaatg gcgccgccgc tcgccgccag tcctcacccg gccgcagcgg atgcggcccc
     3181 gcctggaaat gcacgcgcag cagcgcctgc gcgctgccga ggagtagcag cggcccgatg
     3241 ggcgagtcga tctcgtgcca gtacctcacg cgacactccc cgctcgcgcc atgggcgcgc
     3301 ggcgtgccgt aatagcgcgg ccaccagtcg acggcacctc gatggtcgcg gccgtggcgg
     3361 cgcgccacag gtgcatgacc gcgtaacctc gccagggtcg ccatgcgcgc gcacgttcct
     3421 cgagctcgcg cacactcaat agccgcgtcg cccgcgccgc catgcgacgt aacaccagat
     3481 ct
//
LOCUS       DATDMP010000677         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_328999, whole genome shotgun sequence.
ACCESSION   DATDMP010000677 DATDMP010000000
VERSION     DATDMP010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328999"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VI195_11840"
     CDS             <1..373
                     /locus_tag="VI195_11840"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825126.1"
                     /translation="PIVRTVTFDEVRQICEMVGRHLMRAHPKDITMEWAVEKRTGKIF
                     IDYNMNVRGKTLNVAYSPRGVPGAPLSMPLTWAELEAAEPTDFTINNVFDRLEKSGDR
                     WHDVLESKQSLTKAFGVDREK"
     gene            403..1278
                     /locus_tag="VI195_11845"
     CDS             403..1278
                     /locus_tag="VI195_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY6825127.1"
                     /translation="MAARSIGSLTISFGLVAIPVKLYTATQSSSAVSFNLLHKKDGSR
                     LKQQYVCQKEGVVVERDEIIKGYEFAKDQYVPFTPEEIKALEEVGTRSVEISEFVPIE
                     SIDPVFYDKTYYLAPDKGAAKPYALLTEALKQSGRAGVGRWATRGKGYIVILRPIGDV
                     LAMQQLHYAEDVRPATEVDVPKPEVKATELKLAQQLIDQQTAERFDPTAFKDDTRARI
                     HAAIDKKVEQGTEISVSEVAPEREGKVIDLMEALRASLQRTETAREQVSRLGPRKAPK
                     RVEAPARASRRKRNA"
     gene            1305..>2025
                     /locus_tag="VI195_11850"
     CDS             1305..>2025
                     /locus_tag="VI195_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6825128.1"
                     /translation="MPTYSMRDVQRVLRLSPDTTRNLIKAGFVKPTRGARREYRFSFQ
                     DLIVLRTARALIDAKVPTRRIRRSLESLRRELPESVPLSGLSISAIGEQVVVRDGDIQ
                     RQVDSGQYLLGLEVSIENGVLRVVEHREAAPAPPAASAPNADWFTRALTLESTDPEAA
                     LTAWGHAVDEDAANAAAWTNWGRLLHEQGRTQQAAEVYRRALARVGPDPLLLYNQGVL
                     LEDLGDATAALAAYQSAIAEDP"
BASE COUNT          387 a          639 c          686 g          313 t
ORIGIN      
        1 gccgatcgtg cgcaccgtca ctttcgatga agtgcgtcag atctgcgaga tggtgggccg
       61 gcacctgatg cgggcgcacc cgaaggacat caccatggag tgggcggtgg agaagcgtac
      121 cggcaagatc ttcatcgact acaacatgaa cgtgcgcggc aaaacgctca atgtcgcgta
      181 ttcgccacgc ggagtgccgg gcgcgccgct gtccatgccg ctcacctggg cagagctcga
      241 ggccgcggag ccaacagact ttacaatcaa caacgtattc gaccggttgg aaaagagcgg
      301 cgaccggtgg catgatgtac tcgagtcaaa acagagcctt acgaaggctt tcggtgtcga
      361 tcgtgagaag tagccgagtg tgggctgctt gaaggtgcag gtatggccgc gcgttccatc
      421 ggttctctga caatctcctt cggcttggtc gcgatcccgg tgaagctcta caccgccacg
      481 caatcatcga gcgctgtctc cttcaacctg ctgcacaaga aggatggctc gcgtctcaag
      541 cagcagtatg tgtgtcagaa ggaaggcgtg gtggtggagc gtgacgaaat catcaagggc
      601 tacgagttcg ccaaggacca gtacgttccc ttcacccccg aggagatcaa ggcgctcgag
      661 gaagtcggca cgcgctcggt agagatctcc gagttcgtgc cgatcgagtc gatcgacccc
      721 gtgttctacg acaagaccta ctacctcgcg cccgacaagg gggcggccaa accctatgcg
      781 ctgctcaccg aagcgctcaa gcagtccggt cgcgccggcg tcggccgctg ggcaacgcgc
      841 ggcaagggct atatcgtgat cctgcggccg ataggcgatg tgctcgccat gcagcagctg
      901 cactatgccg aggacgtgcg cccggcgacc gaagtggacg ttccgaagcc cgaggtgaag
      961 gccaccgagc tgaaactcgc gcagcagctc atcgaccagc agaccgccga gcgtttcgac
     1021 ccgacggcgt tcaaggatga cacccgcgcg cgcatccacg cggcgatcga caagaaggtg
     1081 gaacagggca cggagatttc ggtgtcggag gtggcacccg agcgtgaggg caaggtcatc
     1141 gacctgatgg aagcgctgcg cgcgagtttg cagcggaccg agactgcacg cgagcaggtc
     1201 agccggcttg gtccgcgcaa ggcgccgaag cgcgtcgaag ctcccgcgag agcgtcccgc
     1261 aggaagcgta acgcctgagt gcggcaagcc gcgggcgaat ggggatgccc acctacagca
     1321 tgcgggacgt acagcgggtc ctgcggctct cacctgacac gacccgcaat ctcatcaagg
     1381 ccgggttcgt caagcccact cgtggggcac gccgcgagta ccgcttctcc ttccaggacc
     1441 tgatcgtgct acgcacggcg cgcgcgctga tcgacgccaa ggtcccgacg cggcgcattc
     1501 gccgttcact cgagagcctg cggcgcgagc tgcccgagtc ggtgccgctc tccgggctgt
     1561 cgatcagcgc catcggtgag caggtggtgg tgcgcgacgg cgacatccag cgtcaggtcg
     1621 acagcggtca gtacctgttg ggtctcgagg tcagcatcga gaacggagtg ttgcgcgtgg
     1681 tcgagcaccg cgaggcagcg ccggcaccgc cggcggcatc cgcgccgaac gctgactggt
     1741 tcacccgggc gctcaccctc gagagcaccg acccggaggc cgcgctgact gcctgggggc
     1801 acgccgtgga cgaggatgcc gccaacgcgg cggcctggac caactgggga cggctgctgc
     1861 acgagcaggg acgcacgcag caggccgcgg aggtgtatcg gcgcgcgctc gcgcgggtgg
     1921 gtcccgatcc gctgctgctc tacaaccagg gcgtgctgct cgaggatctc ggcgacgcca
     1981 cggcggcact cgccgcgtac cagagcgcca tcgcggagga tccgg
//
LOCUS       DATDMP010000678         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1107535, whole genome shotgun sequence.
ACCESSION   DATDMP010000678 DATDMP010000000
VERSION     DATDMP010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107535"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1279
                     /locus_tag="VI195_11855"
     CDS             116..1279
                     /locus_tag="VI195_11855"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY6825129.1"
                     /translation="MALFAYGFRPLFLLAGVGALLLVPAWALDFAFGVSPGSGWPPTL
                     WHAHEMLFGFIASAVGGFLLTAVPSWTGQKGFAGQPLMVLTALWLAARIMIASSSLWP
                     PLLPAAVDLAFIPALGVLIAGPLLRSRNRNTPLLLVLALLWLADLTFHVGLAHRDAPL
                     ALHALLIGIDVMLVMVTVIGGRIVPAFTAAALRSRGGEGAVTSRPGATAAAVAAMIAV
                     TLGDLAVPDSRLAGMLAAAAAVIQAWRLTQWAGLRTLRQPIVWVLHLAYLWLPLGLGL
                     KSIALLEGAAFSAFWLHALTIGVLSTMILAVMTRAALGHTGRPLVVHPVTALSYALLT
                     GAALVRVFGLALLPLSYPLVILLAALLWTAAFLLFVVVYAPILCRARADGKPG"
     gene            complement(1283..1684)
                     /locus_tag="VI195_11860"
     CDS             complement(1283..1684)
                     /locus_tag="VI195_11860"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEY6825130.1"
                     /translation="MLASHYPEIRLLHIGCVAFSGTLFTVRGAMRIADIAGANHRALR
                     WLSYTVDTALLLAAILLTLIVHQYPFVNAWLTAKLLLLPLYIALGVIALKRARTPAGR
                     LAAYLAALLTFVMIVGVAVEHEPAGWLRLLR"
     gene            1860..>2166
                     /locus_tag="VI195_11865"
     CDS             1860..>2166
                     /locus_tag="VI195_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825131.1"
                     /translation="MQIQDPEFWRAGAISTTAVMLVVLILLTIDSLAATTAGSARVPD
                     YDVINRQIGYAYDWSRGVDVPVLGPAEPLFGETYSPGQARALVDLGKLVIQSRACMDC
                     "
BASE COUNT          280 a          755 c          763 g          368 t
ORIGIN      
        1 cggagcgcgc ggctgcgctt gcccggcgga ggaaatggtt tacgatcgcc cccggtgcgc
       61 cgcatgccga acacctgcga agcccttgcc ggcgagctca cgatcgtctg ctgaagtggc
      121 gctgtttgcc tacggcttcc gtccgctgtt cctgctcgcc ggggtgggcg cgctgctgct
      181 ggtgccggcc tgggcgctcg acttcgcctt cggcgtctcg cccggctccg gctggccacc
      241 gacgctgtgg cacgcgcacg agatgctctt cggcttcatc gcgagcgcgg tcggaggatt
      301 cctgctcacc gccgttccga gctggaccgg gcagaagggc ttcgccggac aaccgctcat
      361 ggtgctcacg gcgctgtggc tcgccgcccg catcatgatc gccagctcct cgctgtggcc
      421 gccactcctg ccggcggccg tggatctggc tttcatcccg gcgctcgggg tgctgatcgc
      481 cggtccgctg ctgcggtccc gcaaccgcaa cactccgctg ctgctggtgc tcgcactgct
      541 gtggctcgcc gacctcacct tccacgtcgg actggcccac cgcgacgcgc cgctcgcact
      601 gcacgcgctg ctcatcggca tcgatgtcat gctggtgatg gtgacggtga tcggcggacg
      661 catcgtgccg gcgttcaccg ctgcagcgct gcgctcgcgg ggtggcgagg gtgcggttac
      721 ctcgcgcccg ggcgcgaccg ctgcggcggt agcggccatg atagccgtga ccctcgggga
      781 cctcgccgta ccggacagtc gcctggcggg gatgctggcg gcggcggcgg ccgtgatcca
      841 ggcgtggcgg ctgacgcagt gggcaggcct caggacgctt cgtcagccga tcgtctgggt
      901 gctgcacctc gcctatctct ggctgccgct cgggctcggc ctcaagagca tcgcactgct
      961 cgagggcgcg gcgttctcgg ccttctggct gcacgcactg acgatcggcg tgctgtcgac
     1021 gatgatcctg gcagtcatga cccgcgccgc gctcggtcac accggccgtc cgctcgtcgt
     1081 ccacccggtc accgcgctgt cgtacgcgct gttgaccggg gccgccctgg tgcgggtctt
     1141 cgggctggcg ctgctgccgc tcagctatcc gctggtcatt ctcctcgcgg cgctgctctg
     1201 gaccgcggcc ttcctgctgt tcgtggtggt gtacgcaccg atcctctgca gggcgcgtgc
     1261 cgacggcaag cccgggtagc gctcagcgga gcagccgcag ccatcccgcc ggctcgtgct
     1321 ccaccgccac gccgacaatc atgacgaagg tgagcagcgc cgccaggtag gcggccaggc
     1381 ggcccgcagg cgtgcgtgcg cgcttcaggg cgatgacgcc cagagcgatg tacagcggca
     1441 gcagcagcag cttggccgtc agccacgcgt tcacgaacgg atactggtgc acgatcagcg
     1501 tcagcaaaat cgccgccagc agcagcgcag tgtcgacggt gtacgagagc cagcgcagcg
     1561 cgcggtgatt ggcgccggcg atatcggcga tacgcatggc gccccggacg gtgaacaggg
     1621 tgcccgagaa cgccacgcag ccgatgtgca gcagccggat ctcggggtaa tgggaggcca
     1681 gcatgagcct tgcggggtcg tccgggcacc attgtgaccc tcacgggggc cgcaggccag
     1741 tcgccgcccg ggggcgcggc ctggcagtag gtgtaaatgc gaataggttt gcgcgcccgg
     1801 ctggggtctg atcgcccccg atcctctcgc cggcgccggg gcccggcatg gaggcagcga
     1861 tgcagattca ggatcctgaa ttctggcgtg ccggtgcgat cagcaccacc gcggtcatgc
     1921 tggtcgtgct catcctgctc accatcgaca gcctggcggc gacgaccgcc ggcagtgcgc
     1981 gggtgccgga ctacgacgtc atcaaccggc agatcggcta tgcgtacgac tggtcgcgcg
     2041 gcgtcgatgt gccggtgctc gggcctgccg aaccgctgtt cggcgagacc tacagcccgg
     2101 ggcaggcgcg cgcgctggtc gacctcggca agctcgtcat ccagagccgg gcgtgcatgg
     2161 actgtc
//
LOCUS       DATDMP010000679         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_884277, whole genome shotgun sequence.
ACCESSION   DATDMP010000679 DATDMP010000000
VERSION     DATDMP010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884277"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VI195_11870"
     CDS             <1..330
                     /locus_tag="VI195_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825132.1"
                     /translation="ARHLDDLATLLDLTEAQKAQVQSILEEQHAKMKQSFEQARASGT
                     HPDWQQMKALHQQAQQEMLQKLAPVLSATQLKKFEIIQQHMMHAHMGHHGPDGAGPAS
                     PAPPAQN"
     gene            complement(379..1104)
                     /locus_tag="VI195_11875"
     CDS             complement(379..1104)
                     /locus_tag="VI195_11875"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY6825133.1"
                     /translation="MARLFVSDVHLDAAAPQATEQFLSFLREQAAAAEALYILGDLFE
                     AWVGDDDEEPGNEQVCRALHELTTGGVACFALHGNRDFLLGAGFCARSGCRVLSDPVI
                     AQLDGERVLLTHGDALCTDDGPYQELRSIVRDRAWQQRFLALPRAHRELLADEARAGS
                     RRHTARTVPDIMDVNGAAVAAAFRAARVRRIVHGHTHRPGVHELEVDGAPAQRIVLGA
                     WYEQGSFLHYEHGRYQLRTLPRY"
     gene            complement(1109..1771)
                     /locus_tag="VI195_11880"
     CDS             complement(1109..1771)
                     /locus_tag="VI195_11880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6825134.1"
                     /translation="MLIKLPTRIGLLASLLLSSLLLSAVCRAAEPTTQVRVTTTMGEF
                     VIEVRNDRAPLTAANFLRYVREGFYTNTLFHRVIANFVLQGGGHDATTQQLKPTHDSV
                     FNESGNGLQNKRGTVGLARGESPHSGNAQFYVNLVDNPDLDPVPTRWGYTVFGRVVQG
                     MDVIEHIGETPTGAMGPFKSDSPLKPVVIEKMEIISGQAAAPAPVTPVTPAAPNTILS
                     PK"
     gene            1889..>2412
                     /locus_tag="VI195_11885"
     CDS             1889..>2412
                     /locus_tag="VI195_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY6825135.1"
                     /translation="MSTSASAPPQQGAPVITRFAPSPTGELHLGNARTALFNQLLARR
                     AGGRLLLRIEDTDSERSRDAHTHALMADLAWLGIEWDAGPDREDGRGPYRQSQRGALY
                     TQELAALERGGAVYPCFCTPLELSLSRRAQLAAGRPPRYAGTCRELPAAERARRIAAA
                     VPATLRFRVPGAER"
BASE COUNT          417 a          806 c          825 g          364 t
ORIGIN      
        1 gcgcgccact tggatgatct ggctacgctg ctcgatctga cggaggcgca gaaagcgcag
       61 gtgcagagca tcctcgaaga gcagcacgcg aagatgaaac agagcttcga gcaggcgaga
      121 gcctccggca cccacccgga ctggcagcag atgaaagcgc tgcaccagca ggcgcaacag
      181 gagatgctgc agaagctcgc gccggtgctc tcagcgacgc agctgaagaa gttcgagatc
      241 atccagcagc acatgatgca tgcccacatg ggccatcacg ggcccgacgg cgcggggcca
      301 gcctcaccag cgccgccggc acagaactga tgcggcaagc ggcgctgccg ccgtgcggga
      361 ttctggagac gcgcggtgtc agtagcgcgg cagcgtgcgc agctggtaac ggccgtgctc
      421 gtagtgcagg aagctgccct gctcgtacca ggctccgagc acgatgcgct gtgccggcgc
      481 gccgtcgacc tccagctcgt gcacgccggg gcggtgcgta tggccatgca cgatgcgccg
      541 cacgcgagcg gcgcggaagg cagccgcgac cgccgcgccg ttgacgtcca tgatgtccgg
      601 aaccgtgcgc gcggtatggc ggcggctgcc ggcgcgggct tcgtcggcga gcagctcgcg
      661 atgcgcgcgt ggcagtgcga ggaagcgctg ctgccaggca cgatcgcgca caatgctgcg
      721 cagctcctga tagggaccat cgtcggtgca tagcgcatca ccgtgcgtca gcagcacacg
      781 ctcaccatcg agctgcgcga tcaccgggtc gctaagcacg cggcagccgc tgcgcgcgca
      841 gaaacccgcc ccgagcagga agtcacgatt gccgtggagc gcgaagcagg cgacgccgcc
      901 ggtagtgagc tcatgcagcg cgcggcacac ctgctcgttg cccggttcct cgtcgtcgtc
      961 cccgacccac gcctcgaaga gatcgccgag gatatagagc gcctcggccg ccgccgcctg
     1021 ctcgcgcagg aagctcagga actgctcggt ggcttgcggg gcggcggcgt cgaggtgcac
     1081 gtccgagacg aacaggcgcg ccatcgactc acttcggcga caggatcgta ttcggggcgg
     1141 ccggagtgac cggagtcacc ggcgctggag cggccgcctg gccggagata atctccatct
     1201 tctcgatgac gacgggcttg agcggcgaat cgctcttgaa aggtcccatc gccccggtgg
     1261 gcgtctcacc gatgtgctcg atcacgtcca tgccctgcac cacgcggccg aagaccgtgt
     1321 aaccccagcg ggtcggtacc ggatcgagat cgggattgtc gaccagattc acgtagaact
     1381 gcgcgttgcc cgaatggggt gactctccgc gcgcgagtcc gacggtgccg cgcttgttct
     1441 gcaggccatt gccggattcg ttgaagacac tgtcgtgagt cggcttcaat tgctgggtgg
     1501 tggcgtcatg cccgccaccc tggagcacga aattcgcgat gacgcggtga aacagggtat
     1561 tggtgtagaa gccttcgcgt acgtagcgca gaaagttcgc ggcggtgagc ggggcacgat
     1621 cgttgcgcac ctcgatgacg aattcgccca tggtggtggt aacccgcacc tgggtggtcg
     1681 gctccgcggc gcggcacacc gcggacagca gcagcgaact gagcagcaag ctggcgagta
     1741 agccgatccg cgtcgggagc ttgatgagca tcgatcctcc acctggccac agagaagttc
     1801 gggacagagt accggcggca aattctatag cagccggcga gctgagggcg ctttgttgag
     1861 attcgtgact ttcccgtaac atggcgtcat gagcacgagc gccagcgcgc caccgcagca
     1921 gggcgcgcca gtgattaccc gtttcgcccc gagtccgacc ggcgagctgc acctgggaaa
     1981 tgcgcgcacc gcgctcttca accagctgct cgcacggcgc gccggcggcc gtcttttgct
     2041 gcgcatcgag gacaccgaca gcgagcgcag tcgggatgcc cacacgcacg cgctcatggc
     2101 cgacctcgcc tggctcggca tcgaatggga cgcgggaccg gaccgcgaag atggccgcgg
     2161 gccctatcgt cagtcccagc gcggtgcgct ctacacgcag gagctggctg cgctcgagcg
     2221 cggcggcgcc gtctacccct gcttctgcac gccgctcgag ctgtccctct cacgccgcgc
     2281 ccagctcgcc gccggtcggc cgccacgcta cgcgggcaca tgccgggagc tgcccgcggc
     2341 cgagcgcgcg cggcgcatcg ccgccgctgt tcccgctacg ctgcgctttc gcgtgccagg
     2401 cgctgagcgc at
//
LOCUS       DATDMP010000680         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2107358, whole genome shotgun sequence.
ACCESSION   DATDMP010000680 DATDMP010000000
VERSION     DATDMP010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107358"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VI195_11890"
     CDS             complement(<1..829)
                     /locus_tag="VI195_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6825136.1"
                     /translation="MRNAPSPALALLACAALASCAVGPNYHRPPAANPQHFKEAEGWK
                     PAEPREAASGTEWWSIYGDAKLDELEKQIDISNQTLKASEAAWRQATALASQARAGLL
                     PTVGVSATGTRSGGPGGRATGAATGGTVTPLTHPVNEFELFGSATWDIDIWGKIRRTI
                     ESDVANAQASEADLAAARLSAQATLASDYIQLRIADEQQQLLAKTVDGYKRSLEITQN
                     QYHVGVVAKADVITAQTQLGGAQSQLIAIGVTRATLEHAIAVLIGKAPADFSLPQETL
                     "
     gene            complement(826..>2654)
                     /locus_tag="VI195_11895"
     CDS             complement(826..>2654)
                     /locus_tag="VI195_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6825137.1"
                     /translation="LHGQGRMFRASEQAFEAVLGAYRRSLRWALDNRGLVMLMLAATV
                     VLNVYLYVKIPKGFFPQQDTGRLIGGIQADQAISFQSMSRKLRQFEAIVQADPAVESV
                     VGFTGGGSQGGGQTNSGFLFASLKARSQRKVSADEVIGRLRPKLNQVAGARLFLQAAQ
                     DIRTGGRQSLSQYQYTLQADSIADLYHWAPQLTDALQREPTLTDVNSDQQQNGQEIEL
                     VIDRDTAARLKLNPAAIDNTLYDAFGQRYVSTIYKALNQYHVVMEVAPEYWQSPDTLR
                     NLWISTAGGTVSGSQSTNAVAGTVISTQTAANSTAAAIAADTARNQATNSLANTGHGA
                     VSTGAAVSTAAEEMVPLAAFSHYAPGNTPLAVNHQGPFVATTLSFNVAPNHSLSDAQA
                     AIARAVNEIRMPSTVHGSFEGTGRTYEESLGNEPILILTALAAVYIVLGVLYESYIHP
                     VTILSTLPSAGAGAVLALRLFNTEFSIMALIGVILLIGIVKKNAIMMIDFALDAQRQG
                     HPPAEAIYTAALLRFRPIMMTTMAALLGALPLAFGSSEGSETRQPLGISIVGGLIVSQ
                     LLTLYTTPVMYLYLDRFGRWSSTLWMRLHPEGPPAAPAQVRL"
BASE COUNT          419 a          846 c          910 g          479 t
ORIGIN      
        1 cgagtgtttc ctgaggcagc gagaaatctg ccggcgcctt gccgatcagc actgcgatgg
       61 cgtgctcgag ggtcgcgcgc gtcacaccga tcgcgatcag ctgcgactgg gcgcccccga
      121 gctgcgtttg cgcagtgatg acgtcggcct tggcgacgac gcccacgtgg tactggttct
      181 gcgtgatctc gagcgagcgc ttgtagccat ccaccgtctt ggcgagcagt tgttgctgct
      241 catccgcgat ccgtaactgg atgtagtcgc tggcgagggt ggcctgcgcg gagagccgcg
      301 cggcggcgag atcggcttca ctcgcctgcg cgttggccac atcgctctcg atcgtacggc
      361 ggatcttgcc ccagatgtcg atatcccagg ttgcgctgcc gaagagctcg aactcgttca
      421 ccggatgtgt gagcggggtg accgtccccc cggttgccgc gccggttgcg cgtccccccg
      481 ggccgccgga gcgcgtgcca gtggcgctca cgccgaccgt cggcagaagt cccgcacgcg
      541 cctggctcgc gagcgcagta gcctggcgcc aggcggcctc tgaggccttg agcgtctggt
      601 tcgagatatc gatctgcttc tcgagctcat cgagcttcgc atcgccgtag atcgaccacc
      661 actctgtgcc actcgccgct tcgcgtggct cggcgggctt ccagccctcg gcctccttga
      721 agtgctgtgg attggcggcc ggcgggcggt gatagttggg gccgaccgcg cagctcgcga
      781 gcgcggcaca ggcgagcagc gcgagggcgg gggagggagc gtttctcata gtcgaacctg
      841 cgccggcgcc gcgggcggac cctccggatg caaccgcatc cacagtgtgc tggaccagcg
      901 cccgaagcgg tccagataca gatacatgac cggagtggtg tacagcgtca gcagctggct
      961 gacgatgagg ccaccgacga tcgagatgcc gaggggttgg cgcgtctccg agccctcgct
     1021 gcttccgaat gcgagcggca gtgcacccaa gagcgccgcc atggtcgtca tcataatcgg
     1081 ccggaagcgc agcagcgccg ccgtatagat cgcctcggcc ggaggatgtc cctgacgctg
     1141 cgcatcgagc gcgaagtcga tcatcatgat cgcgttcttc ttcacgatcc cgatgaggag
     1201 aatcacaccg atcagcgcca tgatgctgaa ctcggtattg aagagacgca gtgccagcac
     1261 ggcgcccgcg ccggcggagg gaagcgtgga gagtatggtg accgggtgga tgtagctctc
     1321 gtacagcacg ccgagcacga tatagaccgc cgccagcgcc gtgaggatca gaatcggctc
     1381 attccccagc gactcctcgt aggtgcgccc ggtgccctca aagctgccgt gcacggtcga
     1441 gggcatgcgg atctcgttga ccgcgcgggc gattgccgcc tgcgcatcgc tcagtgagtg
     1501 attcggtgcc acgttgaagg agagcgtggt cgcgacgaac ggtccctgat ggttgacggc
     1561 gagcggcgta ttgcccggtg cgtagtggct gaaggcagcg agcggcacca tctcctcggc
     1621 ggccgtgctg accgccgcgc ccgtcgagac cgcaccgtgg ccagtgttgg cgagactatt
     1681 ggtcgcctgg ttgcgcgccg tgtcggccgc gatcgccgcg gcggtgctgt tggccgcggt
     1741 ctgagtcgag atcacggtgc cggcgacggc gttggtcgac tggctgccac tcactgtgcc
     1801 gccggcagta ctgatccaca ggttcctgag cgtatcgggg ctctgccagt actccggcgc
     1861 cacctccatg accacgtgat actggttgag cgccttgtag atggtcgaga cgtaacgctg
     1921 gccgaaggcg tcgtagagcg tattgtcgat cgcagccgga ttgagcttca gccgtgcggc
     1981 ggtgtcgcga tcgatgacga gctcgatctc ctggccgttc tgttgctgat cggagttgac
     2041 gtcggtgagc gttggctcac gctgcagcgc gtccgtgagc tgcggcgccc agtggtaaag
     2101 gtcggcgatg ctgtcggcct gcagtgtgta ctggtactgc gacaggctct gccgcccacc
     2161 ggtgcggatg tcctgcgccg cctgcaggaa gagccgtgcg cccgccacct ggttgagctt
     2221 cggccgcagc cgaccgatca cctcatcggc gctgaccttg cgctgtgagc gggccttgag
     2281 cgacgcgaag aggaagccgc tgttggtttg tccgccaccc tgcgagcctc cgccggtaaa
     2341 gccgaccacc gactcgaccg ccgggtccgc ctggacgatg gcttcgaact gccgcagctt
     2401 gcgcgacatc gattggaagg agatggcctg gtcggcctga ataccaccga tcagccgccc
     2461 ggtgtcctgc tgcgggaaga agcccttggg gatcttgacg tagagataga cgttcaacac
     2521 cacggtcgcc gcaagcatca acatcaccag gccacggttg tcgagtgccc agcgcagcga
     2581 gcggcggtag gcgccgagca ccgcctcgaa tgcctgctcg ctcgcgcgga acatccggcc
     2641 ctggccgtgc agat
//
LOCUS       DATDMP010000681         3987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1107718, whole genome shotgun sequence.
ACCESSION   DATDMP010000681 DATDMP010000000
VERSION     DATDMP010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107718"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2276)
                     /locus_tag="VI195_11900"
     CDS             complement(<1..2276)
                     /locus_tag="VI195_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6825138.1"
                     /translation="MSAWAIRHPVSPIVLFVVLLFVGTVSFLRVPVTLNPDVAFPVVL
                     VQVAQPGASPQEVETQIMQKIEGAVAGVGNIDNIISLAIEGQSRTFVQFNIGTPIDRA
                     VADVRDAVAKVRVDLPQGIQEPTVQRIDIDGDAIVAYAVSSSTLSNEDLSWFVDNTVT
                     KRLLAVEGVSQVTRGGGVSREIRVELDPARMQALGVTAAEVNQQLRTLNLDSPGGRVQ
                     IGGGEQSIRVLGEARTAKALGDAQIMLHGGRFARLSDLADVKDSVAEVRTLARLNSRP
                     ATMFSVYKAKGTSDVNVLAAVQRELAKIEKENPQVVLSQAYTSVDYTKGTYHSAMEAL
                     VEGSILAVLVVFLFLRNVRATLIAALAIPLSAIPAFAFMQWMGFTLNQVSLLALSLVA
                     GVLVDDAIVEIENIVRHMRMGKSGYRAALDAADEIGLAVVATSATIIAVFLPVSFMGG
                     ITGQYFKQFGLTVAAAVFFSLLVARLITPVLAAYTLKSDKIAAHADGPIMTWYQRVLR
                     WSTRHRWKTLGAGAAFFALSIVGLMLTPTEFIPASDLSWTMLNVELPAGARLEDTSAV
                     SAAAYRILAHQPEVESVVEQIGGDEFGEVRQASLFITLVQPSHRKVTQRQWEDRVIRD
                     LEAIPDARIHFNRNNDNGRDITIYITGDNTQLTEASARKVVDEMRQLPMLRDVGIDGD
                     MPRPEILIKPHLDLASQLGVTVASLSDTIRIATLGELDQYSAKFSLADRQVPIRVSLA
                     EDQRRDLSTLENLPVPTA"
     gene            complement(2298..3581)
                     /locus_tag="VI195_11905"
     CDS             complement(2298..3581)
                     /locus_tag="VI195_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6825139.1"
                     /translation="MGTNPEHIASPPEGRGEPVTWIGRDRPFERLPEPPQASDPSPRV
                     TLDLGGANRWKWTIGALAAVLLIALVAWRLMAGGARPPAALADRGGVPLVSVIVPGQQ
                     AVTSNVTFTGAIAARYDMPIANEGDTGRIVAVYVEPGDRVKRGQLLARIDDSVLVQQV
                     HRLEASLEQARAQAALSAAEYRRAEGVAAAGALSAEDIEKRRATSVTDAANVKVAAAQ
                     LAEAQARLARTHIVAPVEGTVLTRHAEVGQIANPGGDPLFRLESGGEVEMRGQIAEQD
                     LAQVKAGQPATVYLTGITRPFTGQVRLLGAVIDPQTRLGDIRIRLQPDPALRPGAFAR
                     AEVTVDRTQRPVLPQTAVMADSNGSYVLIVNAAGRVERHAVRVSGTTESGVIIAEGLA
                     GTERVVATAGGFLRDGETVNVAPSAPGTNAPIATR"
BASE COUNT          659 a         1325 c         1350 g          653 t
ORIGIN      
        1 ctggcggtgg gcacgggcag attctcgagc gtcgagagat cgcggcgctg atcttcggcg
       61 agactcacgc ggatcggcac ctggcgatcg gcgagagaga acttggcgct gtactgatcg
      121 agctcaccga gcgtcgcgat gcggatggtg tcgctgaggg aggccaccgt gacgccgagc
      181 tgcgaagcca gatcgaggtg cggcttgatg aggatctccg gccgcggcat gtcgccatcg
      241 attcccacat cgcgcagcat cggcagctgg cgcatctcgt ccacgacctt gcgcgcgctc
      301 gcctcggtga gctgcgtatt gtcgcccgtg atgtagatgg tgatgtcgcg cccgttgtcg
      361 ttgttgcggt tgaaatggat gcgggcgtcg ggaatggctt cgagatcccg gatgacccga
      421 tcctcccatt gacgctgggt caccttgcgg tggctcggct gcaccagggt gatgaacagg
      481 ctcgcttggc gcacctcacc gaactcatcc ccgccgatct gctccaccac ggactcgact
      541 tccggctggt gggcgaggat gcgataggcg gctgctgaca ccgccgaggt gtcctcgagg
      601 cgggcgccgg ccggcagctc gacgttgagc atcgtccagc tcagatcgga ggcgggtatg
      661 aactccgtcg gcgtcagcat gaggccaacg atcgagagcg cgaagaaagc cgcgcccgcg
      721 ccgagggtct tccagcggtg gcgggtgctc cagcgcagca cgcgctggta ccaggtcatg
      781 atcgggccgt cggcgtgcgc ggcgatcttg tcggatttga gggtgtaggc ggccagcacc
      841 ggggtgatga gtcgcgccac cagcagcgag aagaacaccg ccgccgcgac cgtgaggccg
      901 aactgcttga aatactgccc ggtaataccg cccatgaagc tcaccggcag gaatacggca
      961 atgatggtcg cggaagtcgc cactacggcg aggccgatct catccgccgc atcgagtgcg
     1021 gcgcggtagc cgctcttgcc catgcgcatg tggcgcacga tgttctcgat ctcgacgatc
     1081 gcatcgtcga ccagcacccc ggcgaccagc gagagggcaa gcaggctcac ctggttgagg
     1141 gtgaaaccca tccattgcat gaacgcgaat gccggaatgg ccgagagcgg gatggcgagc
     1201 gccgcaatga gcgtggcgcg cacgttgcgc aggaacagga aaaccaccag cacggcgagg
     1261 atggagccct ccaccaacgc ctccatggcg gaatggtagg tccccttggt gtaatccacc
     1321 gaggtgtagg cctgcgagag cacaacctgg ggattctcct tctcgatctt ggccagctcg
     1381 cgctgcaccg cggcgagcac gttgacgtcc gaggtgcctt tggccttgta gacgctgaac
     1441 atcgtcgccg gcctgctgtt gaggcgcgcg agcgtgcgga cctctgcaac gctgtccttg
     1501 acgtccgcga gatccgacag gcgcgcaaac cggccaccgt gcagcatgat ctgcgcatcg
     1561 cccagcgcct ttgccgtgcg cgcctcgccg aggacccgga tcgactgctc accaccgccg
     1621 atctgcaccc gcccgcccgg cgaatcgagg ttcagggtgc gcagctgctg gttgacctcc
     1681 gctgcggtga ccccgagcgc ctgcatgcgc gccggatcga gctccacgcg gatttcgcga
     1741 ctcacgccgc caccgcgggt gacctgcgac acgccttcga cggccagcag gcgcttcgtc
     1801 acggtgttgt ccacaaacca ggacagatcc tcgttggaga gggtgctcga gctcaccgca
     1861 taagccacga tcgcgtcgcc gtcgatatcg atgcgctgga cggtgggctc ctggataccc
     1921 tgcggcagat cgacccgcac cttggcgacc gcatcgcgca catcggcgac ggcacggtcg
     1981 atcggcgtgc cgatgttgaa ctgcacgaag gtgcgtgact ggccctcgat cgcgagcgag
     2041 atgatgttgt cgatgttgcc gacaccggct accgcgccct cgatcttctg catgatctgg
     2101 gtctcgacct cctggggtga tgcccccggc tgagcgacct gcaccagcac cacgggaaag
     2161 gcgacatccg gattgagcgt caccggcacc cgcaggaatg acaccgtgcc cacgaacagc
     2221 agcacgacga agagcacgat gggggaaacg gggtgtcgga tcgcccacgc agacatgttg
     2281 cgcatgaccg cgcgccccta ccgcgtagcg attggcgcgt tggtgccggg cgccgaggga
     2341 gcgacgttga ccgtctcacc atcgcgcagg aagccgccag cggtcgcgac cacccgctcc
     2401 gtgcccgcaa gcccctcggc gatgatgacg ccgctttcgg tggtgccgga gacgcgtacc
     2461 gcatgtcgct cgactctgcc cgccgcattg acgatcagga cgtaactgcc gttcgagtcg
     2521 gccatcactg cggtctgcgg caacaccggc cgctgcgtcc ggtcgacggt cacctccgcg
     2581 cgcgcgaatg caccgggccg cagcgccgga tcaggctgga ggcggatgcg gatgtcaccg
     2641 agccgggtct gcggatcgat gaccgcgccc agcagtcgca cttgacccgt aaaggggcgg
     2701 gtaatgccgg tgaggtacac ggtggccggc tgcccggcct ttacctgggc gaggtcctgc
     2761 tcggcgatct gcccgcgcat ctccacctcg cccccgctct ccagacgaaa cagcgggtca
     2821 cctccgggat tggcgatctg accgacctcg gcgtggcggg tgagcacggt cccctcgacc
     2881 ggtgcgacga tgtgagtgcg cgcgaggcgc gcctgcgcct cggccagctg cgccgcggcg
     2941 accttgacgt tggccgcatc cgttaccgag gtggcgcggc gtttctcgat gtcctccgcc
     3001 gacagtgcgc ccgccgccgc gacgccctcg gcgcggcgat actctgccgc ggacaaggct
     3061 gcctgggcac gggcctgctc gagcgatgcc tcgagacgat gtacctgctg caccaacacg
     3121 gagtcatcga tgcgcgccag cagctggccg cgtttgaccc gatcgcccgg ctcgacatac
     3181 acggcgacga tccgccccgt gtcgccctcg ttggcaatgg gcatgtcgta acgtgccgcg
     3241 atggcgccgg tgaacgtcac gttggaggtg accgcctgct gtccgggcac gatcacgctc
     3301 acgagcggga cgccaccgcg atcggccagt gcggccggcg gtctggcacc acctgccatc
     3361 agtctccacg ccacgagcgc aatcagcagc accgcagcca gcgcgccgat ggtccatttc
     3421 cagcgattcg cgccaccgag atccagggtt acccgcgggc tcggatcgga cgcttgggga
     3481 ggctccggga ggcgctcgaa cgggcgatcg cggccgatcc aagtgaccgg ctcgccccgg
     3541 ccttcagggg ggctcgcgat gtgttccggg ttcgtgccca cgcggtgttc ctttggtcct
     3601 gcccagcaaa cggccgccct tcaaatgctt ggcgggccgc tcgcccggag tgtaggacta
     3661 ccgcgctccg aatccatcac cggaaacgcc aggcagacgc gtgtgggcga cgggccgagg
     3721 cggggggcag tgacgatgcg ccgctccgtg acgccggggg cgcgcagtgt acggctccct
     3781 accggggata gcaaaaaaga gcggggaacg tgccggtgca actccttgag cggtctgtgc
     3841 tattccatgc ctgcttgctc gcagggggga cacggtgtct gatttgcctg cagcggccgc
     3901 gcaagagcca atgggagccg tgtttctgag ttacgtctcg cggagtcgcc gtgaaggtgc
     3961 gacagccggc ttctgcagac gctctca
//
LOCUS       DATDMP010000682         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1216974, whole genome shotgun sequence.
ACCESSION   DATDMP010000682 DATDMP010000000
VERSION     DATDMP010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216974"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VI195_11910"
     CDS             <1..406
                     /locus_tag="VI195_11910"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY6825140.1"
                     /translation="RVVGILPRFMADLEWGHKGLTDLQLVEDLRTRKHMMLTLSQAAI
                     ALPGGSGTLEELLEAITLKRLGLYLNPILLVNTRGFFTPLLELLARAVEERFMDPRHL
                     LMWQVVDAPDEVPAALANAPAWSVEARQFAAV"
     gene            complement(422..2050)
                     /locus_tag="VI195_11915"
     CDS             complement(422..2050)
                     /locus_tag="VI195_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825141.1"
                     /translation="MSPRLWTGVIVIAGLVGVACAQEPAAGEGGARPPAPAGGKPAPL
                     PAQVSQLCRIDIKGEKKEQWMRLPTAQASVTQHRLSLGSKSIEYSATAGTLIVRDDED
                     RPIASIGYVAYTRRDGKSGGVRPITFAFNGGPGSSSLWLHMGVLGPKRVLVSDPTPTP
                     AAPFRTADNEFSLLDVSDVVMIDPVGTGLSHAVCDHKDEEFWAVDPDVDSVSRFIAQY
                     VSDNNRWTSPKYLLGESYGTTRAAAIVNYLHAHRSLTFNGLVLVSVATDVEAIFAELP
                     GNDRPYAVYLPGYAAVAWYHHMVPGQPASLEPFLAEVRQYATGPYTAALLKGDTLSNE
                     ELDVVAQKVHDYIGLPVEYLKQARLRVSENEFAHELLKARGLTVGRLDGRFTGPTSDP
                     LAKETDYDPQSSAISAAFAAAFLDYYHGELRFGQGETYHTTNFGIGEKWKWTHHTERG
                     DQPIVNSGVDLADTLIKDPNLRVLVLNGYYDLATPFSGTEYVMMHLGVPREVAAHVQM
                     RYYEAGHMMYVHPPSLAKMKRDLSAFIESTTRAQ"
     gene            2156..2842
                     /gene="trmB"
                     /locus_tag="VI195_11920"
     CDS             2156..2842
                     /gene="trmB"
                     /locus_tag="VI195_11920"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY6825142.1"
                     /translation="MTAIDHPRSIRSFVTRSGRITEAQQRALTQLWPRYGIEFSPQPL
                     DARALYGRDVPRTLEIGFGNGENLAALACAHPERDFLGVEVHRPGVGRLLREIESQQL
                     TNVRLICHDALEVIAQQIAPGWLEEILIFFPDPWPKKRHHKRRLVQNAFAQLLAARLA
                     AGGVLRIATDWRPYALDMLTTLAAIAQLHNLAADGTFVTRTAERQPTRFERRGAELGH
                     EVYDLAFRRA"
BASE COUNT          489 a          998 c          977 g          521 t
ORIGIN      
        1 gcgggtggtc ggcatcctgc cgcggttcat ggcggatctc gaatggggcc acaagggtct
       61 caccgacctg cagttggtcg aggatctgcg cacgcgcaaa cacatgatgc tgacactcag
      121 ccaggcggcg attgcgctac cgggcggctc gggtacgctc gaggagctgc tcgaagcgat
      181 taccctcaag cggctcgggc tctacctcaa tccaatcctg ctggtgaaca cgcgcggctt
      241 cttcaccccg ttgctcgagc tgctcgcccg cgcggtggag gagcgcttca tggatccccg
      301 tcacctgctg atgtggcagg tggtagacgc gccggatgag gtgcccgctg cgctcgcgaa
      361 cgcgcccgcc tggagcgtcg aggcgcgcca gttcgcggcc gtatagggga gccggcggca
      421 gtcactgcgc acgggtggtc gactcgatga aggcgctgag atctcgcttc atcttggcga
      481 gcgacggcgg gtgcacgtac atcatgtggc ccgcctcgta gtagcgcatc tgcacgtgcg
      541 ccgcgacttc gcgcgggacg cccaggtgca tcatgacgta ctcggttccc gagaacggcg
      601 tcgccaggtc gtagtagccg ttcagcacca gcacgcgcag gttcggatcc ttgatgagcg
      661 tgtcggcgag atcgacgccc gagttgacga tcggctggtc gccgcgctcg gtgtggtgcg
      721 tccacttcca cttctcgccg atgccgaaat tggtggtgtg ataggtctca ccctgaccga
      781 agcgcagctc gccgtgatag taatcgagga aggccgcggc aaatgccgcg ctgatcgcgg
      841 acgattgggg atcgtagtcg gtctccttgg cgagcggatc gctggtgggc ccggtgaagc
      901 ggccgtccag ccggccgacg gtgagcccgc gcgccttgag cagctcgtgc gcgaactcgt
      961 tctcggagac tcgcagccgt gcctgcttga ggtactccac cggcagaccg atgtagtcgt
     1021 gcaccttctg cgccacgaca tcgagctcct cgttggagag cgtatcgccc ttgagcagcg
     1081 ccgcggtgta cgggccggtg gcgtactgcc gcacctcggc caggaacggc tcgagcgagg
     1141 ccggctgacc cggcaccatg tggtgatacc aggctaccgc cgcatacccc ggcaggtaca
     1201 ccgcgtacgg gcggtcgttg ccaggcagct cggcgaagat ggcctcgacg tccgtcgcta
     1261 ccgatacgag caccaagccg ttgaaggtga gcgagcggtg cgcgtgcagg taattgacga
     1321 tggccgcggc gcgcgtggtc ccatagctct cgcccagcag atacttcggc gaagtccagc
     1381 ggttgttgtc gctcacatat tgcgcgatga agcggctcac cgaatcgacg tccgggtcga
     1441 ccgcccagaa ctcctcgtcc ttgtgatcgc agaccgcgtg gctgagcccg gtaccgaccg
     1501 gatcgatcat caccacatcg ctcacatcga gcaggctgaa ttcattgtcc gccgtacgga
     1561 aaggggcggc cggagtgggc gtggggtcgg agaccaggac gcgcttgggt cccagcacgc
     1621 ccatgtgcag ccacagcgac gatgagcccg gaccgccgtt gaacgcaaaa gtgatggggc
     1681 gcacgccgcc tgacttcccg tcgcggcgcg tataggccac atagccgatg ctggcaatcg
     1741 gcctgtcctc gtcatcgcgc acgatgagcg tgccggcggt tgcgctgtat tcgattgatt
     1801 tgctgccgag gctgagtcga tgctgtgtta ccgatgcctg cgccgtcggc aagcgcatcc
     1861 actgctcttt cttctcaccc ttgatgtcga tgcggcacaa ctgtgacacc tgcgccggca
     1921 agggcgcagg cttaccgccg gctggtgcag gcggtcgtgc accgccttcg cccgcggcgg
     1981 gctcttgtgc acacgctacc cccaccaatc ctgcaatgac gatcacaccc gtccacagcc
     2041 gcggcgacat ctgcacctcc actctgttgc gtgtcgagct cgcccgcggt ttgctgcgga
     2101 cgtcgcgcgc gcttatacca tgtcgacggc gccagccgct gtaagatcgg cgtacatgac
     2161 ggccatcgac catccgcgct cgatccgctc cttcgtgacg cgctccgggc ggatcaccga
     2221 ggcgcagcag cgcgcgctca cacagctgtg gcccagatac ggcatcgagt tctcgccgca
     2281 gccgctcgat gcacgtgcgc tctatggacg cgacgtccca cgcacgctcg agatcggctt
     2341 cggcaatggt gagaacctcg cggcgctcgc gtgcgcccac ccggagcgcg atttcctcgg
     2401 tgtggaagtg catcgccctg gcgtcggcag gctgctgcgc gagatcgaat cgcagcagct
     2461 taccaatgtg cggctcatct gccacgatgc gctcgaggtg atcgcgcaac agatcgcccc
     2521 gggatggttg gaggagattc tcatcttctt tcccgacccg tggccgaaga agcgccatca
     2581 caagcgccgc ctggtgcaaa acgcattcgc acagctgctg gcggcgcgtc tggcggcggg
     2641 cggcgtgctg cgcatcgcca ccgactggcg accctatgcg ctcgacatgc tgaccactct
     2701 cgcggccatc gcgcagctgc acaacctggc ggccgatggc accttcgtga cccggactgc
     2761 cgagcgccaa ccgacgcgct tcgagcggcg cggtgccgag ctcggccatg aggtctatga
     2821 cctcgccttc cggcgcgcct gaagctcagc tcacaccgaa ggcctggcgc agcccatcga
     2881 gcacgaactg caccgcgagc gcgcccagga ttaccccgag caggcggctc gcgacgttgg
     2941 cgccggtgac gccgatcacg cgcatcagcg gatcggcgag cagca
//
LOCUS       DATDMP010000683         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1995635, whole genome shotgun sequence.
ACCESSION   DATDMP010000683 DATDMP010000000
VERSION     DATDMP010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995635"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VI195_11925"
     CDS             <1..541
                     /locus_tag="VI195_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6825143.1"
                     /translation="LNSTNGTYVNGKLIKKHALQHGDVITVGHHQLRFVEDDEQQDEF
                     EKTMVIQPSARPVEKVRSATEVADRASGALTATGRTRALADGAAAVKKAKLQVLSGAF
                     AGREVELSKALTTLGRPGVQVAAITRRAEAYYIVHVDSGKADDFPLLNGTPIGAQATR
                     LNDNDVIQLAGVKMGFFLN"
     gene            complement(538..1614)
                     /gene="alr"
                     /locus_tag="VI195_11930"
     CDS             complement(538..1614)
                     /gene="alr"
                     /locus_tag="VI195_11930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY6825144.1"
                     /translation="MSRLIRAVIDTRALRHNLTTVRERARRARVMAVVKANAYGHGLV
                     PTALALAAADAFAVARLEEGLALRAAGITQPIVLLEGVFSAEQLREAARHGFELVVHD
                     ALQIELLEEMTGAHRFVLWIKIDTGMNRLGFPPQEFPAALARVQRLQPAPLEIRLLTH
                     LACADERDNRKTGQQLARFQAATRGLGFSVSIANSAAIFGTVPLHCDWVRPGLALYGA
                     SPFADALGTQLGLEPVMSLETSVIALRQVARGETVGYGGAWSAPRDSRIAIVAAGYGD
                     GLPRNLPGGTPVLVAGERAPLVGRVSMDMIAVDVSALPALHVGTPVVLWGRGLPVEEI
                     ARHAGTIPYELLCSVSQRVPIELG"
     gene            complement(1611..>2668)
                     /gene="dnaB"
                     /locus_tag="VI195_11935"
     CDS             complement(1611..>2668)
                     /gene="dnaB"
                     /locus_tag="VI195_11935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY6825145.1"
                     /translation="AQIVRERSLLRQLIEAGTQIAAAVYNNDGQSARELVDRAEQRVF
                     EIAEGSFRGLEGARAVRELLPAVIDQIDEWHANPDKLRGLPTGFTDFDKLTGGLRPGD
                     LVIIAGRPSMGKTTLAINMAEYAAVHPGTRASVAVFSMEMPSEQVITRMLASVGGVPL
                     SNLRSGRISDEDWVRITGATSQLSEAKIFVDETPALNPTELRARARRIKREHGLSLVV
                     VDYLQLMQVPGTQENRATEIAEISRGLKALAKELAVPVVALSQLNRGVEQREHKRPVM
                     SDLRESGAIEQDADIILLIYRDEVYDRNTTRKGIAEIDLVKHRNGEIGTFLLTFQGQF
                     TRFANFVPDSYAEGVLR"
BASE COUNT          414 a          932 c          941 g          381 t
ORIGIN      
        1 tctcaacagc accaacggca cctacgtcaa cggcaagctc atcaagaagc atgcgctgca
       61 gcatggcgac gtgatcaccg tcggccatca ccagctgcgc ttcgtcgaag acgacgagca
      121 gcaggacgaa ttcgagaaga ccatggtgat ccagccgtcg gcccgaccgg tcgagaaggt
      181 gcgcagcgcg accgaagtcg ccgaccgggc gtccggggcg ctcaccgcca ccggccgcac
      241 gcgcgccctg gccgacggcg ccgccgcggt caagaaagcg aagctgcagg tgctgtcggg
      301 tgcctttgcc ggccgcgagg tcgagctcag caaggcgctc actaccctcg ggcgtcccgg
      361 ggtgcaggtg gcggcgatca cccgccgtgc ggaggcctac tacatcgtgc acgtcgacag
      421 cggcaaggcg gacgactttc cgctgctcaa cggcacgccg atcggcgcgc aggcgacccg
      481 gctcaacgac aacgacgtga tccagctcgc cggcgtgaag atgggattct tcctgaacta
      541 gccgagctcg atcggcaccc gctggctcac gctgcagagc agctcgtacg ggatcgtgcc
      601 cgcgtggcgc gcgatctcct ccaccggcag cccgcggccc cacagcacca ccggcgtgcc
      661 gacgtgcagc gccggcagcg cagagacatc caccgcgatc atgtccatcg aaacccgccc
      721 gacgagcggc gcgcgctcgc ccgccaccag caccggcgtg ccgcccggga gattgcgcgg
      781 cagcccatcg ccgtatcccg ccgcgacgat ggcgatgcgg gaatcgcgcg gcgcgctcca
      841 ggcgccgccg tagccgaccg tctcgccgcg ggcgacttgc cgcagcgcga tcaccgaggt
      901 ctcgaggctc atcaccggct cgagcccgag ctgggtcccg agggcatcgg cgaacggcga
      961 ggcgccgtag agcgccagcc cggggcgcac ccagtcgcag tgcagcggca ccgtgccgaa
     1021 gatggcagcc gaattggcga tgctgacgga aaagccgagc ccgcgggtcg ccgcctggaa
     1081 gcgcgcgagc tgctgcccgg tcttgcggtt gtcgcgctcg tcggcgcagg cgaggtgggt
     1141 gaggaggcgg atctcgagcg gcgccggctg caggcgctgc acgcgcgcga gcgccgcggg
     1201 gaattcctgg ggcggaaacc cgaggcggtt catgccggtg tcgatcttga tccacagcac
     1261 gaagcggtgc gcgccggtca tctcctcgag cagctcgatc tgcagcgcat cgtgcaccac
     1321 cagctcgaag ccgtggcggg cggcctcgcg cagctgctcg gcgctgaaca cgccctcgag
     1381 cagcacgatc ggctgggtga tgccggcggc gcgcagcgcc aggccctcct cgaggcgcgc
     1441 caccgcgaaa gcatcggccg cggcgagcgc cagcgcggtg ggaacgagcc cgtggccgta
     1501 ggcgttcgcc ttgaccaccg ccatgacccg ggcgcggcgg gcgcgctcgc ggacggtggt
     1561 gaggttgtgc cgcagcgcgc gcgtgtcgat gaccgcgcgg atcaggcggc tcaccgcagc
     1621 acgccctcgg cgtaggagtc gggcacgaag ttggcgaagc gggtgaactg cccctggaag
     1681 gtgagcagga aagtgccgat ctcgccgttg cggtgcttga cgaggtcgat ctccgcgatg
     1741 cccttccggg tggtgttccg gtcgtagacc tcgtcgcggt agatgagcag gatgatgtcg
     1801 gcgtcctgct cgatcgcgcc actttcgcga aggtccgaca tcaccggccg cttgtgctcg
     1861 cgttgctcga ctccgcggtt caactgcgag agcgcgacca ccggaaccgc cagctccttg
     1921 gcgagcgcct tcaggccgcg ggaaatctcc gcgatctccg tggcgcggtt ttcctgggta
     1981 cccggcacct gcatcagctg caggtaatcg accacgacga gactcaggcc gtgctcgcgc
     2041 ttgatgcgcc gggcgcgggc gcgcagctcg gtgggattca gcgccggagt ctcgtcgacg
     2101 aagatcttcg cctccgagag ctggctggtg gcgccggtga tgcgcaccca gtcctcgtcc
     2161 gagatgcggc cgctgcgcag gttcgagagc ggcaccccgc cgaccgaggc cagcatgcgc
     2221 gtgatcacct gctcggaggg catctccatg ctgaagaccg ctaccgaggc gcgcgtgccg
     2281 ggatgcaccg cggcgtactc ggccatgttg atggcgagcg tggtcttgcc catcgagggg
     2341 cgcccggcaa tgatcaccag gtctccgggg cggagcccgc cggtgagctt gtcgaaatcg
     2401 gtgaagccgg tcggcagccc gcgcagcttg tcgggattgg cgtgccattc gtcgatctgg
     2461 tcgatcaccg cgggcagcag ctcgcgcacc gctcgcgcgc cctcgaggcc gcggaagctc
     2521 ccctcggcga tctcgaacac gcgctgctcg gcgcgatcga ccagctcgcg cgcgctctgc
     2581 ccgtcgttgt tgtacacggc tgcggcgatc tgcgtgccgg cctcaatcag ctgccgcagc
     2641 agcgagcgct cgcgcacgat ctgggcgt
//
LOCUS       DATDMP010000684         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_2219217, whole genome shotgun sequence.
ACCESSION   DATDMP010000684 DATDMP010000000
VERSION     DATDMP010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219217"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VI195_11940"
     CDS             complement(<1..1968)
                     /locus_tag="VI195_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825146.1"
                     /translation="MHVAFSKLYPMLMPALLAWWAMSAAAAPFDLAGPTLEVEVTHGA
                     RTLPIAQVPNLAVGDHLLVKADLPAGGPTHYLMVAAFLRGATNPPPAEWFFSCETWAR
                     RCAKEGLTLTVPQDAEHLLLFLAPQTGGAYSTLVDTVRGRPGVFVRTSQDLNQAALDH
                     ARLEAYLAAVRHLGDADPSQLKESVPLLSRSLAIKVDESCLAKIPVLQGPCLAQGRES
                     LILDDGRGVSLAQTLTSGPASDLAMEASNAPQLKSGYYGPFIGSIIDIAKILDSLHTA
                     RYQYFPALTSMHGRQLALSLNAPPSFHDPKSVLVVALPPVEAPQFPALHAVNPNDILC
                     ASKDPLLLPVEGAPVVFATAYAHDAVLHWNGKDGTAVDLPARPDPTRGGFAATLPAGT
                     APTGNATLQAQWGFDRYTGPSFKLADPQAQVWRLAPAESAELTIGRTNTVHLRADTVS
                     CLDEVTLVDSAAKPHKVEWKKTHPNEVEVSVPLQDAATGDLTLRVKQFGRADAQTLAL
                     HAAAIESPRPGVELISRTVQWPNAGLENAVQLSNDSTLPLGARLNFSIRAQSPAGFTT
                     DEKLEVATTDGVFSALLGVGTGQVMLQSRNVAVVTLDPAKALGASAFGPLQFRRIVAG
                     VGSDWIPLVTLVRLPKLSGVYCTADPQVGCSL"
     gene            complement(2066..>2613)
                     /locus_tag="VI195_11945"
     CDS             complement(2066..>2613)
                     /locus_tag="VI195_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY6825147.1"
                     /translation="KTMGLELAVQFGWRLPQVVFYPTGGGTGLIGMWKAWQELAELGW
                     IDGPLPRLIAVQASGCAPIVRAFESGSETAAPWENAHTIAAGIRVPTALGDFLILRAL
                     RESGGCALAVSDEQIDSARAHVARADGLLLSPEGAATYAAYLEATRSGRIAPTDEVVL
                     FNCASGLKYPMPRVVERMAVG"
BASE COUNT          445 a          865 c          883 g          420 t
ORIGIN      
        1 cagcgaacag ccgacctgcg gatccgctgt gcaatagacg ccactcagct tgggcaagcg
       61 caccaaggtc acgagcggta tccagtcact gcccacgccc gccacgatgc gccggaattg
      121 aagcgggccg aacgccgagg ctccgagcgc cttggcggga tcgagcgtga ccaccgcgac
      181 atttcggctc tgcagcatca cctggccggt gccgacgccg agcagtgccg agaacacgcc
      241 atccgtcgtc gcaacttcga gcttctcgtc ggtcgtaaag cctgccggcg attgggcacg
      301 aattgaaaag ttgagcctcg ccccgagcgg caaggtggaa tcgtttgaga gctgcactgc
      361 attctcgagt ccggcattcg gccactggac cgtccggctg atgagctcca cgcccgggcg
      421 cggtgactcg atggctgcgg catggagcgc gagcgtctgt gcgtccgcgc gtccgaattg
      481 cttcacccgc agggtgagat ccccggtggc ggcatcctgc aacggcacgc tcacctcgac
      541 ctcattggga tgggtcttct tccactcgac tttgtgcggt ttggcagcgg agtccaccag
      601 cgtgacctca tccaggcagc tgacggtatc ggcacggagg tggaccgtgt tggtgcgtcc
      661 gatggtgagc tccgcagatt ccgccggggc gagccgccat acctgcgcct gggggtcggc
      721 caacttgaag ctcgggcccg tgtagcgatc gaatccccac tgcgcttgca gcgtggcgtt
      781 gccggtcggt gcggtccccg caggcagggt ggccgcaaat ccgccgcggg tcgggtcggg
      841 ccgggccggc aggtcgacag cggtgccgtc cttcccattc cagtgcagca ccgcatcgtg
      901 cgcgtaggcg gtcgcgaaca ccacgggcgc gccttcgacc ggcaacaaca ggggatcctt
      961 gctcgcgcac agaatgtcgt tcggattgac cgcgtgcagc gccgggaact gtggcgcttc
     1021 taccggaggc agcgccacca ccagcaccga cttcggatcg tggaaggagg gcggtgcatt
     1081 caggctgagc gcgagctgcc ggccatgcat ggaggtgagc gccgggaagt actggtagcg
     1141 cgccgtatgc agcgaatcga ggatcttggc gatgtcgatg atcgagccga tgaaggggcc
     1201 gtagtagccg gacttcagct gcggggcatt gctcgcttcc atcgccagat cgctcgcggg
     1261 ccccgaggtg agggtctgcg caagcgagac accgcgtccg tcatcgagaa tcagggactc
     1321 gcgtccctgc gcgagacaag gcccctgcag cacggggatc ttcgcgagac agctctcgtc
     1381 caccttgatc gcaaggctgc gcgagaggag tggaaccgat tccttgagct gcgaaggatc
     1441 ggcgtcgccg agatgccgca cggctgcgag gtaggcctcg agccgcgcgt ggtcgagcgc
     1501 ggcctggttc aggtcctgcg aggtgcgcac gaacaccccg ggccgcccgc gcaccgtgtc
     1561 gacgagcgtg ctgtacgcgc cgccggtctg cggcgcgagg aagagcagga ggtgctccgc
     1621 gtcctgcggc accgtcaacg tgaggccctc cttggcgcag cggcgcgccc aggtctcgca
     1681 ggagaagaac cactccgccg gcggcggatt ggtagcgccg cggaggaatg cggccaccat
     1741 cagataatga gtgggcccgc cggcaggaag atccgccttg accaggagat gatcgccgac
     1801 ggcgagattc ggcacttgag cgatcggcag ggtacgcgcg ccgtgggtca cttcgacctc
     1861 gagagtcggc cccgccagat caaaaggagc ggcagccgcg ctcatcgccc accacgcgag
     1921 cagcgccggc atcagcatcg ggtacagttt ggaaaaagcg acgtgcacgc ggatgtgtcg
     1981 gtccctggct acactctctc gtccttctcg ctgcacactg tagcgcatct acgcgctttt
     2041 tcgcgtgatt gcgctcgcag aagactcagc cgactgccat gcgctccacg acccgcggca
     2101 tcgggtactt cagcccgctc gcgcagttga agagcaccac ctcatcagtg ggcgcgatgc
     2161 gacctgagcg cgtcgcctcg agataggcgg cgtaggtcgc ggcgccttcg ggcgagagca
     2221 gcagtccatc cgcgcgcgcc acgtgcgctc gggcggagtc gatctgctca tcggacacgg
     2281 ccagcgcgca gccgccgctc tcgcgcaagg cgcgcaggat gaggaaatca ccgagcgctg
     2341 tcggtacgcg gatgccggcg gcgatcgtat gcgcgttttc ccacggcgcc gcggtctcac
     2401 tccctgactc gaaggctcgc acgatcggcg cgcaaccgct cgcctgcacg gcaatgagcc
     2461 gcggcagggg gccatcgatc cagccgagct cggcgagctc ttgccaagcc ttccacatgc
     2521 cgatgagtcc ggtgccaccg cctgtcgggt agaacaccac ctgcggcaga cgccacccga
     2581 actgcaccgc gagctcgagt cccatcgttt tct
//
LOCUS       DATDMP010000685         3977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_551852, whole genome shotgun sequence.
ACCESSION   DATDMP010000685 DATDMP010000000
VERSION     DATDMP010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551852"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..474)
                     /locus_tag="VI195_11950"
     CDS             complement(28..474)
                     /locus_tag="VI195_11950"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEY6825148.1"
                     /translation="MRWLVGLVAGVLCIALRPSLSEAAEWKRLYSAADRSVEIDLADI
                     RRNGDVVLAWVKFSYDREQADAGKGRYRSMLQLWAYQCGLGRHALMQFAEYAGASGEG
                     KVVASDARDRYQWSYPEPETIGEAAMKLACRRAPKAGVQNASATAL"
     gene            complement(624..1502)
                     /locus_tag="VI195_11955"
     CDS             complement(624..1502)
                     /locus_tag="VI195_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825149.1"
                     /translation="MTETEELARLAMGGRALRRRRLSRALLARLIGDQYDTDEEDFEG
                     EEGTGEDDAQRIVRLLIGSRLLRRRRLRNLLLAHLLRERGGAQEEEDEGEEASGEEGA
                     DKDHKLVRLLIGSRILRRRRARRMLLAHLLRERSEAQEEDEEEGEDEFAASGDGDRRL
                     LRLLIGSRILRRRRARRALLAHLLRERSEAEEDEDEGEDELGEADDSEHRLIRLLVGS
                     RMLQRRRVRRMLLAHLLRERGEAEEDEEESEDEFGDGGDKEHRLLRLLAGHRIRQRRR
                     ARRLLMGHLLREHEEA"
     gene            complement(1522..2091)
                     /locus_tag="VI195_11960"
     CDS             complement(1522..2091)
                     /locus_tag="VI195_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825150.1"
                     /translation="MSTAASPRHNHLKQRIPVGKVPVGKKNAHKEDKMNEEKMTESAP
                     SAGRLFAFTIDAGTAQIVKLETLDASGARRELSEEEKASLAQAGAEEGLEQFVEKAFE
                     AGIACVLGDDEQQHKSTEAAEEAELRRLLLKPLIEHSPVRRLMQREALNRAILGTLLQ
                     RAVSKAPVDAESAPAAGPQPDRAASAHAN"
     gene            complement(2580..3074)
                     /locus_tag="VI195_11965"
     CDS             complement(2580..3074)
                     /locus_tag="VI195_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY6825151.1"
                     /translation="MSGRRRSVAPLEVIPIGFVESGRRTPQDDYWGGEQACIRLSEEF
                     SPLALEGLVEFSHVEVLFVLHLLDPAGVVCGSRHPRDNPLWPCVGIFAQRASNRPNRI
                     GSTMCRVLRVLDRQLFVADLDAIDGTPVLDLKPVMQEFLPREALRQPEWSRQLMREYW
                     SRRK"
     gene            complement(3071..3784)
                     /locus_tag="VI195_11970"
     CDS             complement(3071..3784)
                     /locus_tag="VI195_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6825152.1"
                     /translation="MWGSRLAILASVSILASRSPLAIAQDNSGAPAADDLRPAQLAWG
                     SDPVKRCPELRESSAEEGAVAVVKFMVGPTGAPSQASVRSSSGSAGFDAAAVRCVLRL
                     HFLPVTRYGDGTAVESWQQTALKWTGAVRVSQSPRCEASAGTASAENPAVVTAAPADM
                     PKGNQPGPTMARAGICVCVDEVGKITQPPVLTNSSGMPQFDKAALELSAAAPYRPATA
                     ANGQSAAGCFRFKVGIDAK"
BASE COUNT          651 a         1398 c         1199 g          729 t
ORIGIN      
        1 ccacgctgct gcaggcccga tgttgtgtca gagagcagtc gcagaggcgt tttgcacacc
       61 ggccttcggc gcacggcggc aggcgagctt catggccgct tcaccgatcg tctcgggctc
      121 ggggtagctc cactggtaac gatcgcgcgc gtcggaggcc accaccttgc cctcgccgct
      181 tgcaccggcg tactcggcaa actgcatgag tgcgtggcgg ccgagtccgc actggtacgc
      241 ccacagctgc agcatcgacc gataccgtcc cttccccgca tcggcttgtt ccctgtcgta
      301 agagaacttc acccaggcca gcaccacatc gccgttgcgg cggatgtccg cgagatcgat
      361 ttccacggat cggtccgccg cgctgtacag gcgcttccat tccgcggcct cggaaagcga
      421 cggtcgcagc gcgatgcaca gcacccccgc caccaggcca accagccacc gcatgtgatc
      481 ctcctcgtgc aggatcctaa cggtggctgc ggcgacactc ccgcgatatg cgtcacagtg
      541 tgcagtccga tcagaaaaac ggccggaggg ggggtcgcct ccggccgcta tatcgcacta
      601 caccttccac gctggagaga tcttcatgcc tcctcgtgct cgcgcagcag gtgccccatc
      661 agcagccggc gggcgcgtct gcgctgccgt atgcggtggc cggcgagcag gcgcagcagc
      721 cgatgttcct tatcgccgcc atcgccgaac tcatcctcac tctcctcctc gtcctcttca
      781 gcctccccgc gctcacgcag caggtgcgcg agcagcatcc gacgcacgcg gcgccgctgg
      841 agcatgcggc tgcccaccag cagccgtatg aggcgatgtt cgctgtcgtc ggcttcaccg
      901 agctcgtcct cgccctcgtc ctcgtcctcc tctgcctcgg atcgctcccg cagcagatgc
      961 gcaagcagtg cgcgccgcgc acgccgccgc cgcaggatac ggctgccgat gagcagacgc
     1021 agaagacgac gatcgccatc accgcttgcg gcgaattcat cctcgccttc ctcctcgtcc
     1081 tcctcttggg cctcgctacg ctcgcgcagc agatgcgcca gcaacatccg ccgcgcacgc
     1141 cgccgccgca ggatgcgact gccgatcagc aagcgaacca gcttgtggtc tttgtcggca
     1201 ccctcctcgc ccgatgcttc ctcaccttca tcctcctctt cctgcgcacc gccgcgctcg
     1261 cgcagcaggt gcgcgagcag caggttgcgc aagcggcgac ggcgcagcag gcgactgccg
     1321 atcaacaggc gcacgatgcg ctgcgcgtcg tcttcgcctg tgccttcttc accctcgaag
     1381 tcctcctcgt ccgtgtcgta ttggtcaccg atgaggcgcg ccagcagcgc acggctcaac
     1441 cgccggcgac gcagagcgcg gccacccatg gcaaggcggg caagttcctc tgtttcggtc
     1501 ataagtcctc ctgtcgttca gtcaatttgc atgagcggac gcagcgcgat ccggttgcgg
     1561 ccctgccgcg ggcgcgctct cggcgtccac tggtgccttg ctcacggcac gctgtagcaa
     1621 tgtgccgaga atcgcccggt tgagcgcttc gcgctgcatc agccgcctta ccggactgtg
     1681 ctcgatgagt ggcttcagca gcaggcgccg cagttccgcc tcctcggcgg cctccgtgct
     1741 cttgtgctgc tgctcgtcgt cgccgagcac gcaggcgatc ccggcctcga aggctttctc
     1801 gacgaattgc tcgagtcctt cctcggctcc tgcctgcgcc aggctcgctt tttcctcctc
     1861 cgagagctcg cggcgcgcgc cgctggcgtc gagggtttcg agcttgacga tctgcgcggt
     1921 acccgcgtct atggtgaacg cgaacagtct tcccgccgat ggtgcgctct ccgtcatctt
     1981 ctcctcgttc atcttgtcct ccttatgggc gttcttcttc ccgaccggaa ccttcccaac
     2041 tggaatcctc tgcttgagat gattgtgacg agggctggcg gctgtactca tttcaaactc
     2101 cttccgactc tgtgcgggcc ggatgctttc gatcgatcac tcgggaatga cggcgggatg
     2161 tgagcgctgc agcgagcccg tccagcaggg tgacgcagcg agtgcgcctg aaagagagaa
     2221 tgcaagtccc tgcgtttccg cgccacgagg tgcgacgcct taccatcctg ataacgccgt
     2281 ttaaactctg ctgaagatcc gccccggttc tgctcgggcc gcagcaggcg acacatccgc
     2341 cgcgaggtcc cgatcacttc agcggcggca ttatcgcacc gccacgccgc gcgaagacta
     2401 atcttttgtg actatctttc tggctgtgtc gcgcgcgctc acggcaacgc agagaagatc
     2461 agcgacgcgt gtggcttcga agctcgagct caagtcagtg ggtgctcgtt ctgtgagacc
     2521 tgcaacggcg gttgcggtct cgctcgtggt tcgcgcagcg cgcgcgcatc agcgcacctt
     2581 catttccgcc gcgaccagta ctcgcgcatg agctgccgag accactcggg ctgtcgcagc
     2641 gcctcgcgcg gcagaaactc ctgcatcacc ggcttcaggt cgagcaccgg agtgccatcg
     2701 atcgcatcga gatccgctac gaacagttgc ctatccagta cgcgcaaaac acggcacatg
     2761 gtgctgccga tccgattcgg ccggttgctc gctcgctgcg cgaatattcc aacgcacggc
     2821 cagagcggat tgtcgcgtgg atgtcgcgaa ccgcagacaa ctcccgcggg atcgagcagg
     2881 tgaagaacga acagcacctc gacatgagag aactccacca gaccctcgag cgcgagcggc
     2941 gagaattcct cgctcaggcg gatgcacgcc tgctcgccac cccagtagtc gtcttgcggc
     3001 gtccgccggc cgctctcgac gaaccctatc gggatgactt ccagcggcgc aacactccgg
     3061 cggcgcccgc tcattttgcg tcgattccga ccttgaagcg gaagcagcca gcagccgatt
     3121 gcccgttcgc cgcggtggcg gggcgatacg gtgccgcagc ggagagctcg agagcagcct
     3181 tgtcgaactg cggcataccg gatgagttgg tcaaaacggg aggctgggta atcttcccca
     3241 cctcgtctac gcagacgcag atcccggctc tcgccatcgt ggggcctggc tgattgcctt
     3301 tgggcatgtc ggccggggcg gcggtaacca ccgccggatt ttccgcagac gccgtccccg
     3361 cggacgcctc gcagcgtgga ctctgcgaca ctcttaccgc accggtccat ttcagcgccg
     3421 tctgctgcca ggattcgacc gccgtgccat cgccgtaacg ggtaaccggc aggaagtgca
     3481 gcctgaggac gcaccgcact gcggctgcat cgaagcctgc cgagcccgat gaggaacgga
     3541 cggacgcctg cgatggcgca ccggtcggcc cgaccatgaa cttcaccacc gccacggcgc
     3601 cctcctcggc gctgctctcc cggagctccg gacaacgctt gaccgggtca gacccccagg
     3661 ccagctgggc gggacgaaga tcatccgcag cgggtgcgcc cgagttgtcc tgcgcgatgg
     3721 cgagcggcga acgggacgcg agtatcgaca ctgaggcaag aatcgcgagc cgactgcccc
     3781 acatggaatc cccgccgttc tctgctgcgg tctagtgaag cgcgcgcctc aggggtattg
     3841 gtgctccacc acggcgcgta ccgggtgcgt atcgtagacg agcacgatgg actggaggtg
     3901 tccggtgctc ggatcgaagt tgaaaacatc ggcgcagtcg aatgacacgc gcgagccatc
     3961 cttcagccgc cagtcgt
//
LOCUS       DATDMP010000686         2895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_551872, whole genome shotgun sequence.
ACCESSION   DATDMP010000686 DATDMP010000000
VERSION     DATDMP010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551872"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VI195_11975"
     CDS             complement(<1..394)
                     /locus_tag="VI195_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY6825153.1"
                     /translation="MKTFLGVDGGGSKTRFLLIDEGGKVLGTHTAGSAYHLEIGLGTL
                     EAMLARGIADTLQQAGMAPSALTFAFLGLPAYGEDRSLQARLDAAPAAALPSGRYRCG
                     NDMVCGWAGALAGADGINIVAGTGSIAYG"
     gene            complement(391..1554)
                     /locus_tag="VI195_11980"
     CDS             complement(391..1554)
                     /locus_tag="VI195_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY6825154.1"
                     /translation="MDVLGLSAALSQANGAYWTAREIAQQPQLWREIAQLVASDAALA
                     GFLGQLRTDEPLRVVLTGAGTSSFIGECLAPALARRGWSRAEAVASTDIVASPASFLV
                     RDTPTLMVHFARSGNSPESVAALALAEQCIDRCYHLIVTCNDQGELYRRAGAMRHAHA
                     VLLPQASNDQGFAMTSSFTGMLLAAGAALQVLPMSDARAALLSGAGTRVLHSCEPLLT
                     SLVQARFARVVYLGSKELKGLAREAALKMLELTDGRVIAIADSPLGFRHGPKTILNGG
                     TLVVVFLSNDEHARRYEVDLLTELRRDAVAGRVIALAGREDGGAHADTLVLSEDAVAL
                     SDLELCLPYAVFAQVLGMLYSMSLGLSPDNPNAAGTVNRVVQGVSIYPYGRTP"
     gene            complement(1579..>2895)
                     /locus_tag="VI195_11985"
     CDS             complement(1579..>2895)
                     /locus_tag="VI195_11985"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6825155.1"
                     /translation="SIPHFMQGAGGSYPVVLPQLSASQLAGFLKSLDGKPNPQFCPSL
                     PCTTPFDYALTLPQVNPYNSYSVTEKTLSGYLEADFATSRWSGNLGVRVVHTKTTAGT
                     ASAVPTAIWAYQQPGPTATWNVVYGTSQPIGANGSYTLALPSLNLAYWAVPDTLQLRF
                     GAAETMARPNLSQLAPTSNNNAINGDPTLNYDGTAGLRPVKAWQGDLSIEWYYARHSA
                     LTAAVFYKRITNDIYTGVTTSVDLGTQKYLGGPPGTVPGTPFLWTISAPANGAESIYS
                     GVEFTWQHIMENGFGTRMQYTATKTRSYDQSGAFVGAINAAPPTTWSISLIYDKGPWS
                     ADVTWDHQSSYTAYCSQCTDVPGWPAISDAFDWVTASLHYRFLKGFEVFAEGKNLSNS
                     VARSYLNGNPLLPWAPGQLVGASESGTGVGYSAYGRTYVLGIAYTR"
BASE COUNT          490 a          919 c          991 g          495 t
ORIGIN      
        1 ccccataagc gatcgagcct gtgcccgcca cgatgttgat gccgtcggcg ccggcgaggg
       61 caccggccca gccacacacc atgtcgttgc cgcagcgata gcgtccgctg ggaagtgccg
      121 ctgcgggtgc ggcatcgagc cttgcctgca gcgagcggtc ctcgccgtac gccggcagtc
      181 cgagaaaggc gaaggtgagg gccgaaggtg ccatgccggc ctgctgcaga gtgtccgcga
      241 tgccgcgcgc cagcatggcc tcgagtgtcc cgagaccgat ctcgagatga taggcggacc
      301 ccgcggtgtg cgtgccgagc accttgccgc cttcgtcgat gaggaggaag cgcgtcttgc
      361 tcccaccgcc atcgacgccc agaaaggttt tcacggcgtg cggccgtagg gatagatcga
      421 gactccctgc acgacacggt tgaccgtacc cgcggcgtta ggattgtcgg gtgacagtcc
      481 gagcgacatc gagtacagca tgccgagcac ctgagcgaat accgcgtagg ggaggcacag
      541 ctcgaggtca ctgagcgcca ccgcgtcctc gctgagcacc agagtgtcgg cgtgcgcgcc
      601 gccgtcctca cggcccgcga gcgcgatgac gcgcccggcc acggcatcgc ggcgcagctc
      661 cgtgagcagg tcgacctcgt agcggcgcgc atgctcatcg ttgctcagga ataccactac
      721 caacgtgccg ccgttgagaa tcgttttcgg cccgtggcgg aatccgagtg gggaatcggc
      781 gatcgcaatt acccggccgt cggtgagctc gagcatcttg agcgccgctt cgcgtgcgag
      841 tcccttgagc tccttgctgc cgaggtacac gacacgcgcg aacctcgcct gcaccaggct
      901 ggtcagcagc ggctcgcagg aatggagtac gcgcgtgccc gccccgctga ggagcgccgc
      961 gcgtgcatcg ctcatcggca gcacctgcaa cgcggcgcct gccgcgagca gcatgccggt
     1021 gaagctcgaa gtcatcgcga agccctgatc attgcttgct tgggggagca gcaccgcatg
     1081 agcgtgacgc atggcgcccg ccctgcggta aagctcgccc tggtcgttgc aggtgacgat
     1141 gagatggtag cagcggtcga tgcactgctc cgcgagcgcg agcgccgcca cgctctcggg
     1201 gctgttgccg gagcgcgcga aatgcaccat gagggtagga gtgtcgcgca ccaggaaact
     1261 ggcggggctc gccacgatgt cggtgctggc aacggcctcg gcgcgtgacc agccgcgccg
     1321 tgccagcgcc ggcgccaggc attcgccgat gaaggacgag gttccggcac ccgtgagcac
     1381 cacccgcaat ggctcgtccg tccgcagctg cccgagaaac cccgcgaggg cggcgtcgct
     1441 ggcaacgagc tgcgcgatct cgcgccacag ttgcggttgc tgcgcgatct cgcgtgcagt
     1501 ccagtacgcc ccgttggctt gactcaatgc agcgctgagt cccagcacgt ccatggactt
     1561 tcctatcgag gctgcggctc agcgcgtata cgcgatgccg aggacatagg tgcgcccata
     1621 ggcgctgtag ccgacacccg tgccactctc gctggcgccg acgagctgac ccggtgccca
     1681 gggcagcagc ggattgccgt tgaggtagct gcgtgccacg gagttgctca ggttcttgcc
     1741 ctcggcgaat acctcgaatc ccttgaggaa gcggtaatga agcgacgcgg tgacccagtc
     1801 gaaggcgtcc gagatggctg gccagcccgg tacgtcggtg cactgtgagc agtaagcggt
     1861 gtagctcgac tggtggtccc aggtgacatc cgcgctccag ggtcccttgt cgtagatcag
     1921 gctgatcgac caggtggtcg gcggtgcggc attgatggcg ccgacgaagg cgccggactg
     1981 gtcatagctg cgggtcttgg tcgcggtgta ctgcatgcgc gtcccgaagc cattctccat
     2041 gatgtgctgc caggtgaact cgacgccgga ataaatactc tcggcaccat tcgctggagc
     2101 ggagatcgtc cacagaaacg gcgtgcccgg caccgttccc ggcggaccgc cgaggtattt
     2161 ctgcgtgccg aggtccacgc tcgtggtgac accggtgtag atatcgttgg tgatcctctt
     2221 atagaacacc gcggccgtga gcgccgaatg ccgggcgtag taccactcga tggagagatc
     2281 tccctgccac gccttcaccg gccgaagtcc cgcggtgccg tcgtagttga gcgttgggtc
     2341 gccattgatc gcgttgttgt tggaagtcgg cgcgagctgg ctcaagttgg gccgggccat
     2401 ggtctcggcg gctccgaacc gcaactgcag cgtgtccggc acggcccaat aggcgagatt
     2461 caatgaaggt aacgccagcg tatagctgcc gttcgcgccg atcggctgcg aggtgccgta
     2521 gacgacgttc cacgtcgccg tgggacccgg ttgttggtac gcccagatgg ccgtcggtac
     2581 ggcggaggcg gtgccggcgg tcgtcttcgt atgcaccacg cgcacgccaa gatttccgga
     2641 ccaacggctg gtggcaaagt ccgcctccag gtagccggac aaggtcttct ccgtgacgct
     2701 gtaggagttg taggggttga cctgcggcag cgtgagcgcg tagtcgaatg gcgtcgtgca
     2761 cggcagcgac ggacagaact gcggattcgg cttgccatcg aggctcttca ggaacccggc
     2821 aagctggctg gcgctgagct gcggcagcac gaccgggtag gagccgccag cgccctgcat
     2881 gaagtgcgga atgct
//
LOCUS       DATDMP010000687         2850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_441614, whole genome shotgun sequence.
ACCESSION   DATDMP010000687 DATDMP010000000
VERSION     DATDMP010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441614"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VI195_11990"
     CDS             complement(<1..843)
                     /locus_tag="VI195_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6825156.1"
                     /translation="MAGRNFLFVPGPTNVPDRILRAMAVPMEDHRSSKFPELTLQCLA
                     GLKKIFKTASGTPILFPSSGTGCWEAALSNCLDPGDRVLAARFGQFTHLWIDMCQRLG
                     FEVEVLEVEWGGGAPVDRYRAALAADRNHSIKAVLVCQNETATGVASDVAAIRRALDE
                     TRHPALLFVDSVSALASVDFRMDEWGVDVCISGSQKGLMLPAGMGVTCVSEKALAAAK
                     SARSPRCYFDYRDMVSANASGYFPYTPSIPMLYGLREALQLLLEEGLENVFYRHSYIA
                     GGVRA"
     gene            complement(937..2070)
                     /locus_tag="VI195_11995"
     CDS             complement(937..2070)
                     /locus_tag="VI195_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825157.1"
                     /translation="MKRAVAVACVLSLAASAATSAASGAAQTAAAESASGHYTLADFP
                     RVEKIDAHVHLQGRAERFMAQAIADRFHVLTINVDSPEYLPVPAQQQAAISLHERYPG
                     WVAFAATFTVEGFQSPGWSEAVLRHIDEARAHGAVGVKMWKNIGMVLRDPDGSYVMPD
                     DPRFEPIIAHLESASLVLLGHQAEPLNCWLPLEKMTVRSDRDYFREHPQYYMYRHPEM
                     PTHDAILAARDRMLTAHPQLRFDAVHLASLEWDVDRVAAFLDRFPNASVDLAARLVHL
                     EYQAASHPEKVRSFLIRYQDRILYGSDLGYGPEESAPAEIAALHGDWLNDWRFLVTAE
                     PMHSPDFDAGFRGMHLPRSVVDKIYRRNAAALFTNAWNLNAPR"
     gene            complement(2067..>2850)
                     /locus_tag="VI195_12000"
     CDS             complement(2067..>2850)
                     /locus_tag="VI195_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY6825158.1"
                     /translation="TPGSERGLLIEERRRRIRELLRAEGRVTVEALAARFGISLVTVR
                     ADLSTLESAGALTRTHGGALSLPDADQSLDVKQLQHRAEKQRIAAAAADLIRDGETLI
                     LDSGTTTAEIARRIRSLDLKSVNVITNALNIAALLIDVPSVRLIMPGGILRRESNSLS
                     GPMAETALAALRANRLYLGADGVDAQIGVMTPHLAEAELNAKMIGISQQVVVVADSSK
                     FARRNISLIARIEQLHMLITDRAAPADAVEQLRQRGVEVRLV"
BASE COUNT          506 a          914 c          964 g          466 t
ORIGIN      
        1 ggcgcgcacg ccgccggcga tgtagctatg ccgatagaac acattctcca gcccttcctc
       61 gagcagcagc tgcagcgctt cccgcaagcc gtagagcatg gggatcgacg gagtgtaggg
      121 aaaataaccg ctggcgttgg cgctcaccat gtcgcggtaa tcgaagtaac agcgcgggct
      181 gcgggcgctc ttggcggcgg cgagtgcttt ctcgctcacg caggtgaccc ccatgcccgc
      241 cggcagcatc agacctttct gcgagccgct gatgcagaca tcgacacccc actcgtccat
      301 gcggaaatcg acactggcca gcgcgctgac tgaatcgacg aacagcagcg ccggatgacg
      361 cgtctcatcg agcgcgcgac ggatggcggc cacatcgctc gccaccccgg tcgcggtctc
      421 gttctgacac accagcaccg ccttgatgct gtggttccgg tccgcggcga gcgcggcgcg
      481 ataacgatcg acgggcgcgc ctccccccca ctccacctcg agcacctcga cctcgaagcc
      541 gagccgctgg cacatgtcga tccacagatg cgtgaactgc ccgaagcgcg ctgccaacac
      601 gcggtcgccg ggatcgagac agttgctgag cgccgcctcc cagcagccgg taccggaaga
      661 tggaaagaga atgggtgtgc cgcttgcggt cttgaatatc ttcttgagac ccgcgaggca
      721 ctgcagtgtc agttccggaa acttgctcga gcgatggtcc tccatcggca cggccatggc
      781 ccgcaggatg cggtccggca cgttggtcgg acccgggacg aagaggaaat tgcgaccagc
      841 cataagtttt cctttcttct ggcataagac aaagcgaaag cgctgcgggc gctgctgcgc
      901 agcgcattcc accttactac caacatcggt gcggcgctaa cgcggcgcgt tgagattcca
      961 ggcgttcgtg aacagtgccg cggcgttgcg gcggtagatt ttgtcgacca cgctgcgtgg
     1021 caggtgcata ccgcggaagc cggcgtcgaa gtcgggggag tgcatgggct cggcagtcac
     1081 gagaaagcgc cagtcattca gccagtcgcc gtgcaacgcg gcgatctccg cgggagcgga
     1141 ctcctcgggt ccgtacccca gatcgctgcc gtacaggatg cgatcctggt agcggatcag
     1201 gaagctgcgc accttctcgg gatggctggc cgcctggtat tcgaggtgca ccaggcgcgc
     1261 agcgagatcc accgaggcat tcgggaaacg atcgaggaag gcggcgacgc ggtcgacatc
     1321 ccactcgagc gaggcgaggt gcacggcatc gaagcgcagc tgcggatgtg cggtgagcat
     1381 ccggtcgcgc gcggcgagga tggcgtcgtg agtcggcatc tccggatgcc ggtacatgta
     1441 gtactgcggg tgctcgcgga aataatcgcg gtcggagcgc accgtcatct tctcgagcgg
     1501 cagccagcag ttgagcggct cggcctggtg gccgagcagc acgagcgagg cgctctcgag
     1561 gtgcgcgatg atcggctcga agcgcggatc gtccggcatg acatagctgc cgtcgggatc
     1621 gcgcagcacc atgccgatgt tcttccacat cttgacaccc acggcgccgt gcgcgcgcgc
     1681 ttcatcgatg tgccgcagca ccgcctcgct ccagccgggc gattggaagc cttccaccgt
     1741 gaaggtggcc gcgaaagcca cccagccggg ataacgctcg tgcaaggaaa tcgcagcctg
     1801 ttgctgtgcg ggaaccggca ggtactcggg ggaatcgacg ttgatggtga ggacgtggaa
     1861 gcggtcggcg atcgcctgcg ccatgaagcg ctcggcgcgc ccctgcaggt gcacgtgcgc
     1921 gtcgatcttt tcgacgcgcg gaaagtcagc gagcgtgtaa tggccggacg cggactcggc
     1981 cgcggcggtt tgcgccgcgc cggacgcggc gctcgtcgcc gccgacgcgg cgagcgacag
     2041 gacgcacgca acggcgaccg ctcgcttcat accagccgca cctcgacccc gcgctggcgc
     2101 agctgctcga cggcatctgc gggagctgcg cgatcggtaa tgagcatgtg cagctgctcg
     2161 atgcgcgcga tgagcgagat gttgcggcgg gcgaacttgg aggaatccgc caccacgacc
     2221 acctgctgcg agataccgat catcttggcg ttgagctcgg cttcggcgag atgcggcgtc
     2281 atcacaccga tctgcgcatc gacaccatcc gctccgagat acaggcggtt cgcccgcaac
     2341 gcggccagcg cggtttccgc catgggtccc gacagggagt tggattcgcg gcgcagtatg
     2401 ccgcccggca tgatgagacg caccgacggt acatcgatca gcagcgccgc gatgttgagc
     2461 gcattggtga tcacgttgac cgacttgaga tcgaggctgc ggatgcggcg ggcgatctcc
     2521 gcggtagtgg tgcctgaatc aaggatgagc gtctcgccgt cacggatgag gtccgcggcg
     2581 gccgcggcga tgcgctgctt ctcggcccga tgctgcagct gcttgacatc gagcgactga
     2641 tcggcatcgg gcaagctgag ggcgccgccg tgcgtgcgtg tcagcgcgcc ggccgactcg
     2701 agcgtggaga gatccgcacg caccgtgacc agcgagatac cgaaacgcgc ggcgagcgcc
     2761 tcgactgtga cccgtccttc cgcgcgcagc agctcgcgaa tgcgccgcct gcgctcctcg
     2821 atcaaaagtc cgcgctcgct gccgggtgtc
//
LOCUS       DATDMP010000688         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1663617, whole genome shotgun sequence.
ACCESSION   DATDMP010000688 DATDMP010000000
VERSION     DATDMP010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663617"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /gene="rimO"
                     /locus_tag="VI195_12005"
     CDS             complement(<1..1194)
                     /gene="rimO"
                     /locus_tag="VI195_12005"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY6825159.1"
                     /translation="MPHAATRRRRGAPRRQRAPRVGFVSLGCPKALVDSERILTQLRA
                     EGYDVAPSYEAADLVVVNTCGFIDAAIEESLEAIGEALERNGRVLVTGCLGANPQRIL
                     ERHPRVLGVTGPQAYEAVVKAVHAHLPPRHDPFVDLLPPQGVRLTPRHYAYLKISEGC
                     NNRCSFCVIPAMRGRLVSRPVDAVLREAEQLVAAGVKELLVISQDTGAYGADLRYAGG
                     RWRGAHYRSRLIDLAQALGELGVWVRLHYVYPYPHVDELVPLMAERRVLPYLDIPFQH
                     GSPTVLRRMRRPAAAQDTLARIRRWRERCPELTLRSTFIVGFPGETEEEFAELLAWLD
                     EAQLDRVGCFRYSPVAGAAANALPGAVAPGVQEERYRRFMERAAQISARRLAARVGRR
                     VRVLVD"
     gene            complement(1201..1389)
                     /locus_tag="VI195_12010"
     CDS             complement(1201..1389)
                     /locus_tag="VI195_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825160.1"
                     /translation="MAAEPRRPRERRPAIWPWLLMPLVVLAVFCTLLRMHHRPGTPWA
                     GGWAHAAPARDPGAAPAR"
     gene            complement(1379..1966)
                     /locus_tag="VI195_12015"
     CDS             complement(1379..1966)
                     /locus_tag="VI195_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY6825161.1"
                     /translation="MSTAWIAGAWLAATATLVAAQVPSGKSSQKGAGGKFTLTSPDIA
                     PGGKIAERQVFNGFGCRGDNVSPALAWSNPPAGTRCFALLMHDPDAPTGSGWWHWVAY
                     DIPPGVSSLPAGAGDPHRSLMPAGVIQGRTDFGTSGYGGPCPPPGKPHRYYLRLYALK
                     VARLEAPADATAAFIGFNVQANSLGVAELMGVYGR"
     gene            complement(2070..2390)
                     /locus_tag="VI195_12020"
     CDS             complement(2070..2390)
                     /locus_tag="VI195_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825162.1"
                     /translation="MRLKPVRTAVAAALLGAVLSACVVAPGPEYGVAVAYAPPPAQYE
                     VIGVAPAPGYFWIGGGWFWEGGRYAWHPGHWVEPRPGYRWVPRTWVHRRDGWHARGGD
                     WVHR"
     gene            2444..3058
                     /locus_tag="VI195_12025"
     CDS             2444..3058
                     /locus_tag="VI195_12025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6825163.1"
                     /translation="MLHRRRAYNARAGRFGPDYYRRYYYDRRTAVTTRAEMRARARLI
                     AALTAHAGLPVRRILEAGCGTGLLRAALLRLLPRARYTALEASAYLCRRHGWQYGRIE
                     RYRARRPFDLVICYDVLQYLDERAAEGALRNLARLCRGVLYFSALTRIDLERNSDPRR
                     TDADVHLRSARWYRTRLRRHFREAGLGFWVRRGAPLTLWELESQ"
     gene            complement(3030..>4300)
                     /locus_tag="VI195_12030"
     CDS             complement(3030..>4300)
                     /locus_tag="VI195_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY6825164.1"
                     /translation="MLGALCFAELGALLPHAGGEYVYLRAAYGEIPGFLFACNAFVLG
                     GAAVAAYGAAVAIFVSDIHRFDQSWYRHTLHLAGADWTLEFGVRQLIAVAVIGVFALI
                     NCLGVMLGGRVQTLLTAIKVAAILAVAGGVFVFGEAHDWANLRAPPGSTGAGGLAGFG
                     GAMFAALWAYSGWQFLPMAAGEVREPQRTLPRAIVGGTLLVLALYLTINTAYLYALPF
                     WQVASANSTAYPDAPSVAARAVQTFLGGRAAPIAALIFLISTIGSLNGVILSAARVPY
                     AAARDGLFFAPFGRLSPASRVPVTSLVLLALWGALLAASGTFDQLTNMAVMSYALFWI
                     PVSLAVIVLRRRLPQAPRPFRLPGYPVVPLLFALVMVWIVISALLTAPRESIATVVLI
                     ALGLPLYPLFRRRGGRAGAPHATATRVPRG"
BASE COUNT          656 a         1581 c         1553 g          510 t
ORIGIN      
        1 gtcgaccagc acgcgcacgc gccgcccgac acgcgcggcg aggcgccgtg cactgatctg
       61 cgcggcacgc tccatgaatc tgcggtagcg ctcctcctgc accccggggg cgaccgcgcc
      121 gggcagcgca ttcgcggctg cgcccgcgac cggcgagtac cggaagcaac cgacgcgatc
      181 gagctgcgcc tcatcgagcc aggcgagcag ctcggcgaat tcctcctcgg tctctccggg
      241 aaagccgacg atgaaggtgc tgcgcaacgt aagctcgggg cagcgctcgc gccagcggcg
      301 gatgcgcgcc agcgtgtcct gcgcagcggc agggcggcgc atgcgcctga gcaccgtcgg
      361 gctgccgtgc tggaacggga tgtcgaggta cggcagcacg cgccgctcgg ccatgagcgg
      421 cacgagctcg tcgacgtgcg ggtagggata gacgtagtgc aggcgcaccc acacgccgag
      481 ctcgccgagc gcctgcgcca gatcgatcag gcggctgcgg tagtgcgccc cacgccagcg
      541 gccgcccgcg tagcgcaggt ccgcaccgta ggcgccggtg tcctgcgaga tgaccagcag
      601 ctccttcacg cccgccgcca ccagctgctc ggcctcgcgc agtaccgcgt cgacggggcg
      661 gctcaccaga cggccgcgca tggcggggat gacgcagaag ctgcagcggt tgttgcagcc
      721 ctcggagatc ttgagatagg cgtagtgacg cggcgtgagc cgcacgccct gcggaggcag
      781 gaggtcgacg aacggatcgt gccgtggcgg caggtgcgcg tgcaccgcct tcaccaccgc
      841 ctcgtaggcc tggggccccg tgacccccag cacgcgcgga tggcgctcga ggatgcgctg
      901 cggattcgcg ccgaggcagc cggtgaccag cacccgaccg ttgcgctcga gggcctcgcc
      961 gatcgcctcg agcgattcct cgatcgccgc gtcgatgaaa ccgcaggtgt tgacgaccac
     1021 gaggtcggcc gcctcgtagc tcggtgccac gtcgtagccc tcggcgcgca gctgcgtgag
     1081 gatccgctcg gagtcgacca gcgccttggg gcagccgagg ctcacgaagc cgacgcgcgg
     1141 ggcgcgctgg cgccgcggcg cgccgcggcg gcgcctggtg gcggcgtgcg gcatgtgcgg
     1201 ctagcgcgcc ggtgccgcgc cgggatcccg ggcgggcgcg gcgtgcgccc acccgccagc
     1261 ccagggcgtg ccgggccggt gatgcatgcg cagcagcgtg cagaacaccg ccagcacgac
     1321 cagcggcatg agcagccacg gccagatggc cggacggcgc tcgcgggggc ggcgtggctc
     1381 agcggccata cactcccatg agctcggcca cgccgaggga gtttgcctgc acgttgaagc
     1441 cgatgaaggc cgcggtcgca tccgccggtg cctcgagcct ggcgaccttg agcgcgtaga
     1501 ggcgcaggta atagcggtgg ggcttgcccg gcggcgggca cggcccgccg tagccgctgg
     1561 tgccgaagtc ggtgcggccc tggatgacgc ccgccggcat caggctcctg tgcgggtcgc
     1621 cggcgccggc cgggagcgag ctcaccccgg gcgggatgtc gtacgcgacc cagtgccacc
     1681 agccgctgcc ggtcggggca tcgggatcgt gcatgaggag tgcaaagcag cgggtgccgg
     1741 ccggcgggtt gctccaggcg agcgccgggg agacgttgtc gccccggcag ccgaagccgt
     1801 tgaaaacctg ccgctcggcg atcttcccgc ccggtgcgat atcgggactc gtgagggtga
     1861 acttgccgcc cgcacccttc tgcgaggact tcccggacgg cacctgcgcc gccaccaggg
     1921 tcgccgtcgc cgcgagccag gcccccgcaa tccacgccgt gctcatgtgt ccgctccccg
     1981 tccgcagtgg acccgccacc gcagcagcgc agtgtgccgc gcccggcacg cccggtgcaa
     2041 atgcggcacc gggctcgtcc cgccccctct tagcggtgca cccagtctcc accgcgcgcg
     2101 tgccagccgt cgcggcggtg aacccaggtg cggggaaccc agcgatagcc aggccgcggc
     2161 tccacccagt gacccgggtg ccaggcgtag cgcccgccct cccagaacca cccgccgccg
     2221 atccagaaat agccgggcgc cggggccacg ccgatcacct cgtactgcgc cggcggcggc
     2281 gcgtaggcga cggcgacgcc gtattccggc cccggcgcca cgacgcacgc cgacagcacg
     2341 gcgccaagca gcgcagcggc gaccgcggtg cggacaggct tgagtctcat cgtgctgcct
     2401 cctcttgttc ccgggcaggg tgccggaaca tattagcaac aacgtgctcc accgccgtcg
     2461 agcatacaac gcgcgcgccg ggcgcttcgg gcccgactac taccggcgct actactacga
     2521 tcgacgcacc gcggtgacca cccgcgcgga gatgcgcgcc cgcgcgcgcc tcatcgccgc
     2581 cctcaccgcc cacgcggggt tgccggtgcg gcgcatcctc gaggccgggt gcggtaccgg
     2641 gctgctgcgt gcggcgctgc tgcgcctgct gccgcgcgcg cgctacacgg cgctcgaggc
     2701 gagcgcctac ctgtgccgcc gccacggctg gcaatacggg cgcatcgagc gctaccgcgc
     2761 ccggcggccg ttcgacctcg tcatctgcta cgacgtgctg cagtatctcg atgagcgcgc
     2821 ggccgaagga gcgctgcgca acctcgcccg cctgtgccgc ggcgtgctgt acttcagcgc
     2881 gctgacgcgc atcgatctcg agcgcaacag cgacccgcgg cgcaccgatg ccgacgtgca
     2941 cctgcgcagc gcccggtggt accgcacgcg gctgcggcgg cacttccgcg aggcgggact
     3001 cggcttctgg gtgcggcgcg gcgcgccgct caccctctgg gaactcgagt cgcagtagcg
     3061 tgcggcgcgc ccgcgcggcc accgcggcgg cggaacagcg gatagagcgg caggccgagc
     3121 gcgatcagca ccacggtggc gatcgattcc cggggcgcgg tcagcagcgc gctgatcacg
     3181 atccacacca tcaccagcgc gaacagcagc ggcacgaccg ggtagccggg caggcgaaac
     3241 ggacgcggcg cctgcggcag ccggcgccgc agcacgatca ccgcgagcga caccgggatc
     3301 cagaacaggg cgtacgacat caccgccatg ttggtcagct ggtcgaaggt tccggaggcg
     3361 gccagcagcg cgccccagag cgcgagcagt accagcgagg tgaccggcac gcggctcgcc
     3421 gggctcaggc ggccgaaggg cgcgaagaac agcccgtcgc gcgccgcggc gtacgggacg
     3481 cgcgcggcgc tcagaatcac gccgttgagc gagccgatgg tcgagatgag aaagatcagc
     3541 gccgcgatcg gcgcggcgcg cccgccgagg aacgtctgca ccgcgcgcgc cgccaccgag
     3601 ggcgcatcgg gataggcggt ggagttggcg ctcgccacct gccagaaggg cagcgcgtac
     3661 aggtacgcgg tgttgatggt gagatacagc gccagcacca gcagcgttcc tcccacgatc
     3721 gcgcgcggca gcgtgcgctg cggctcacgc acctcgccgg ctgccatcgg caggaactgc
     3781 cagccgctgt aggcccagag cgccgcgaac atcgcgccgc cgaaacccgc gagtcccccg
     3841 gcgccggtgc tgcccggcgg ggcgcgcaga ttcgcccagt cgtgcgcctc accgaagacg
     3901 aataccccgc ccgccacggc gagaatcgcg gccaccttga tggccgtgag cagggtctgc
     3961 acgcgcccgc cgagcatgac gcccaggcag ttgatcagcg cgaacacccc gatcacggcg
     4021 accgcgatca gctgccgcac gccgaactcg agcgtccagt cggcgccggc gaggtgcagc
     4081 gtgtggcggt accagctttg atcgaagcgg tggatgtcgg acacgaagat cgcgaccgcc
     4141 gcgccgtagg cggcgaccgc cgcgccgccg agcacgaagg cgttgcaggc gaacaggaac
     4201 ccgggaatct ccccataggc ggcacgcagg tagacgtact cgccgcccgc gtgcggcagc
     4261 agcgccccga gctcggcgaa gcacagcgcg ccgagcatcg
//
LOCUS       DATDMP010000689         3474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_664072, whole genome shotgun sequence.
ACCESSION   DATDMP010000689 DATDMP010000000
VERSION     DATDMP010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664072"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1800
                     /gene="istA"
                     /locus_tag="VI195_12035"
     CDS             265..1800
                     /gene="istA"
                     /locus_tag="VI195_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012211046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY6825165.1"
                     /translation="MFQYRAVLVRLRQGDSDRDIARTRLMGRPKVAALRAIADRHGWL
                     VADTPLPEDAVIAAAIGEARRARSTISSVEPFRERVTRWAEQGVSGVAIHAALCREHG
                     YRGSYSAVRRMLAVLEAARTPEATVRLQFGPGEAAQVDFGAGPFLLDPARGEQRRTWC
                     FVMTLCFSRHQYAEFVWDQTVATWLGCHRRAFEWFTAVPTRIIIDNPKCAITKACARD
                     PLVQRAYAECAEGYGFKIDPCPPADPAKKGIVEAGVKYVKGNFLPTRAFRDLADLNIQ
                     VRRWVLAEAGVRTHGTTREQPLVLFAVEQPLLHPLPSIAPDLGTWHKVLVHRDCHVQF
                     ERCFYSVPFTLIGASLWLRATDGAVAIYQDYRHVALHARARKPGTRRTVRDHLPPEAQ
                     TFFAHDRDWCVQQAALIGRACATFIERLLADRIVERLRAAQGVLRLAERYGALRLEAA
                     CARALAHDSIFYRTVKTILAGGFDLQPLPTFTQGAPAYAGGARFVRDAETLFGGEPPS
                     SVH"
     gene            1820..2608
                     /gene="istB"
                     /locus_tag="VI195_12040"
     CDS             1820..2608
                     /gene="istB"
                     /locus_tag="VI195_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY6825166.1"
                     /translation="MHPIPELVPYLKQLRLSGILDSLDARNRQALDSKLAYTEFLALL
                     IEDEVARREQKKFTLRLRRAAFRASKTLEQFDFERLPQLNRALVHELATGRYLGEHVP
                     VLIVGPCGTGKSHLAQALGHCAIRQGVDVLFTTCAQLVTTLNAARATGGYDRKLASLA
                     RVPLLVIDDFGLKPLRPPADEDLHDLIAERYETAATIVTSNLDFTEWDQAFPANRLLA
                     AATLDRLRHNAYCLVLDGQSYRAPKLPPAMSKSKIANTSKTTHS"
     gene            2684..3373
                     /locus_tag="VI195_12045"
     CDS             2684..3373
                     /locus_tag="VI195_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEY6825167.1"
                     /translation="MRQRTRESFQQFFTLIGKELSERIEFVCSDMWKPYLDLIRQHCT
                     HALNILDRFHVVAKMNLALDDVRAAEARRMAQGGYEPLLKRSRWCLLKRPENLTDLQR
                     IKLRDLVRYNLQSVRAYLLKEQFQQLWEYDSPIWAGKFLDAWCTQTLRSRIEPMKKFA
                     RTVRAHRELLLNYFRARKQFSSGVIEGLNNKAKVTMRKAYGFRTFRSTELALYHVLGK
                     LPEPHLTHSFY"
BASE COUNT          604 a         1169 c         1061 g          640 t
ORIGIN      
        1 gggcctggaa gcgtggcggc atccctgatc gaacgcggtt gtcagcgatc ggcatattgg
       61 agccactttg tgatcggcat atagaagccg gggcgtgatc ggcatatggg agccatttgg
      121 tgatcgccat aatggagcca gccgctgatc ggcatattgg agccagctga ggtggggacg
      181 ggcatctcga attgagcgca ctgccgctac tctctcggac ttttgtccag agggaggtca
      241 gcgatggggc gcaggagatt cgagatgttc cagtatcgag cggttctggt gcgcttgcgc
      301 cagggcgatt ccgaccgcga cattgcacgg acgcggttga tgggccggcc caaggtcgcg
      361 gcattgcgcg cgatcgcgga tcggcacggt tggctggtcg ccgacacgcc gttgcccgag
      421 gacgccgtga tcgctgcggc gatcggcgag gcgcgccggg cgcgcagcac tatttccagc
      481 gtcgagccgt ttcgcgagcg cgtcacccgc tgggcggaac agggcgtgag tggcgtggcg
      541 atccacgccg cgctgtgtcg cgagcacggc taccgcggca gctactcggc ggtgcgccgc
      601 atgctcgccg tgctcgaagc cgcgcgcacg ccggaagcta cggtgcgttt gcagttcggt
      661 cccggcgaag ccgcgcaggt cgatttcggc gccggtccgt tcctgctcga tccggcgcgc
      721 ggcgagcaac gccgcacctg gtgcttcgtg atgacgctgt gcttctcgcg ccatcagtac
      781 gccgagtttg tctgggatca gacggtagcg acctggctcg gctgtcatcg ccgcgcgttc
      841 gagtggttca ccgcggtgcc gacgcggatc atcatcgaca accccaagtg cgcgatcacc
      901 aaggcgtgcg cacgcgatcc gctggtgcaa cgcgcctacg ccgagtgcgc cgaaggttac
      961 ggcttcaaga tcgacccgtg tccgcccgcc gatccggcca agaaagggat cgtcgaggcg
     1021 ggggtcaagt acgtcaaggg caacttcttg cccacgcgcg cgtttcgcga tctcgctgat
     1081 ctcaacatcc aagtgcgtcg ctgggtgctc gcggaagcgg gcgtacgcac gcatggcacc
     1141 acgcgggagc agccgctcgt gctcttcgct gttgagcaac ccctgctgca cccgttgccg
     1201 tcgatcgcac ccgatcttgg cacctggcac aaggtgctgg tgcatcgcga ctgtcatgtg
     1261 cagttcgagc gctgcttcta ctcggtcccg ttcaccctca tcggtgcgtc gctgtggctg
     1321 cgcgccaccg atggcgcggt ggcgatctat caggactatc ggcacgtcgc actgcatgcg
     1381 cgcgcccgca agccgggcac gcggcgcacc gtgcgcgatc acctgccgcc ggaagcgcaa
     1441 accttcttcg cgcacgaccg cgactggtgc gtgcagcagg cggcgctcat cggtcgggcg
     1501 tgcgcgacct tcatcgagcg gctgctcgcc gatcgcattg tcgagcgcct gcgcgccgcg
     1561 cagggcgtgc tgcggcttgc tgagcgctac ggcgcgctgc gcctggaagc agcgtgcgcg
     1621 cgcgcactcg ctcatgacag catcttctat cgcaccgtca aaacgatcct ggccggcggc
     1681 ttcgacctgc agccgctgcc gaccttcacg caaggcgcgc cggcctacgc cggcggcgcg
     1741 cgtttcgtgc gcgacgccga gacgctcttc ggcggcgaac ccccgtcctc cgttcactaa
     1801 ccccagggag tcttcttgca tgcatcccat tcccgagctc gtgccgtatc tcaagcagct
     1861 gcggctgtcc ggcatcctcg attcgctcga cgcgcgcaac cggcaagcgc tcgacagcaa
     1921 actcgcctac accgaattcc tggccctgct gatcgaggat gaggtcgcac ggcgcgagca
     1981 aaagaagttc acgctgcgtt tgcggcgcgc cgcgtttcgt gccagcaaaa cactcgagca
     2041 gttcgacttc gagcgcctgc cgcagctcaa ccgggcgctc gtgcacgagc tcgccaccgg
     2101 ccgctacctc ggcgaacacg tgccggtgtt aatcgtcgga ccctgcggca ccggcaaaag
     2161 tcatcttgcg caagcactcg gtcactgcgc gatccgccaa ggcgtcgatg tgctgttcac
     2221 cacctgcgcg cagctggtca ccacgctcaa cgccgcacgc gcgaccggcg gctacgaccg
     2281 caagctcgcc agcctggcgc gggtgccgct gctcgtcatc gatgacttcg ggctgaaacc
     2341 gctgcgcccg cccgccgatg aggatctcca tgacctgatc gctgagcggt acgaaaccgc
     2401 cgcgacgatc gtaacgagca acctcgactt tacggagtgg gatcaggcgt tcccggcgaa
     2461 tcgactcctc gccgcggcca cgctcgatcg cttacgacac aacgcgtact gcctggtgct
     2521 cgacggtcaa tcgtatcggg cgccgaaatt gccgccggcg atgagcaagt cgaagattgc
     2581 caacacatcg aaaacaacgc attcttgacc ccgacgggat gctcgtttct acctcaaaca
     2641 ctggctccat taagccgatc atccccggct ccattacgcc gatctgcgac agcgcacccg
     2701 cgagagcttc cagcagttct tcaccctgat cggtaaggag ctgagcgaac gcatcgagtt
     2761 cgtctgctcg gatatgtgga aaccctacct ggacctgatc aggcagcatt gcacccatgc
     2821 cctcaacatc ctcgatcgct tccacgtcgt cgccaagatg aacctcgcgc tcgatgatgt
     2881 gcgggccgcc gaggcgcgcc gcatggcgca ggggggctat gagccgctgc tcaagcgatc
     2941 ccgctggtgc ctcctcaaac gcccggagaa cctcacggac ttgcagcgca tcaagctgcg
     3001 cgatctggtt cgctacaacc tgcagagcgt gcgcgcttac cttctgaagg aacagttcca
     3061 gcagctctgg gaatacgact cgcccatctg ggccggcaaa ttcctcgatg cgtggtgcac
     3121 ccagaccttg cgctcgagaa tcgaaccgat gaaaaaattc gcgcgcactg tgcgcgctca
     3181 tcgcgaactg ctgctcaact atttccgggc acgcaagcag ttctccagcg gcgtcatcga
     3241 gggtctgaac aacaaagcca aagtcaccat gcgaaaagcg tacggctttc gcaccttccg
     3301 ctctacagaa ctcgcgctct atcatgtact tggcaaatta cccgagccgc accttaccca
     3361 cagtttttac tgacgaaccc tttttttaga atttcattct ccatgcgcag ccgcgcattc
     3421 tcggcccgca ctcgggagag ctccatctgc tccggcgtga tcggcttggt tccc
//
LOCUS       DATDMP010000690         3490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_664108, whole genome shotgun sequence.
ACCESSION   DATDMP010000690 DATDMP010000000
VERSION     DATDMP010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664108"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..702)
                     /locus_tag="VI195_12050"
     CDS             complement(145..702)
                     /locus_tag="VI195_12050"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY6825168.1"
                     /translation="MATPPNRPSLAPEINRAYNPRVRPYQAVIFRWSKPMMSVPRRIL
                     VPLCAMGAVFWAALALAAGQSFQVPLTGNQTVPPIDTGGKGVAHITYDAATRTISWTI
                     NYSGLSGPVTMAHFHGPAAKGENAGVQVWLTKQGSPAENPISGQATLTPEQAQDFSAG
                     KWYVNLHTKDHPAGEIRGQVVPPKS"
     gene            701..1093
                     /locus_tag="VI195_12055"
     CDS             701..1093
                     /locus_tag="VI195_12055"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6825169.1"
                     /translation="MALVQRFTGIFALALSVAAGGAAPSDATVHQLMTRDLIGMPGKE
                     VMMETVVYAPGGKSPPHRHSAQVFVYGLEGSLRMQVQGSPVVIVGPGDTFYEGPDDVH
                     VVSENASQTQPAKFLVVMLKDKPTGSHE"
     gene            1380..2132
                     /locus_tag="VI195_12060"
     CDS             1380..2132
                     /locus_tag="VI195_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEY6825170.1"
                     /translation="MQPGNLTYKEGIDIGGDVAHGSAICVPGDPLREPSPLPEDDRLP
                     GWRAVVAAYYRAMERVAEALMRSIARSLQLPQGYFDDAFRQGLSTLRLIRYPPRAAAE
                     LATVTAPALWIEADATRQHLIGAPHTDSGFITLLAQDGAGGLQARSRDGRWIDVPPLA
                     DTLVVNFGQVLEQWSAGRIRATEHRVLGRGIERYSIGFFYEARADATIAPLPVDSPDS
                     FVPFQYGDFLWRRITSFVEFRGMEGERAQSSR"
     gene            2134..3273
                     /locus_tag="VI195_12065"
     CDS             2134..3273
                     /locus_tag="VI195_12065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6825171.1"
                     /translation="MSLYGIAALPALFWLYLLLGRGQFWRVAAQRVPQLPPAAERHIV
                     ALIPARNEAPVIGMAVASLARQVFNGHIHIIVIDDGSSDGTAEAALDAARTAGALARF
                     TFLRSDKLPHGWTGKLWALSQGVAAATTLEPDYLLFSDADICHGTVSVASLVADAEAN
                     DRDLVSHMVKLSTVTLAERLLIPAFVFFFFKLYPPAWIADSRRKLAAAAGGCMLIRPA
                     ALARAGGLPAIRSHIIDDCALARAIKACGGRIALELAADTSSLRRYGSWAEIGAMISR
                     SAFAQLHHSYLLVAATLVGMFLTYLLPVWLLFAGNPLLVGLGITAWLLMSLCYLPMVR
                     FYGQAPLWSLCLPFTAIFYSGAVIHSAVQHARGRGGVWKGRVQDA"
BASE COUNT          561 a         1122 c         1110 g          697 t
ORIGIN      
        1 cgagcgccgc gaaggtgcgc gttacgcgcc ggtcaaccgc agcattctgg gacccgaccc
       61 ggtgccgtga acgaccgtgc gggagcgcgc gccggagttg cgccgcgcgt ccatagtggg
      121 cgggtcgcgc gcttccgttg atcctcagct cttgggcggc acgacctgcc cacggatctc
      181 tcccgcgggg tgatccttcg tatgcaggtt gacgtaccac tttccagcgg agaagtcctg
      241 cgcctgctct ggtgtgagcg tcgcctgtcc gctgatggga ttttccgccg ggctgccttg
      301 tttagtgagc catacctgca cgccggcgtt ctcgccctta gccgccgggc catggaaatg
      361 tgccatggtg accggtcccg acagaccgct gtagttgatc gtccagctga tcgtgcgcgt
      421 cgcagcatcg taggtaatgt gggcaactcc cttgccgccg gtgtcgatgg gcggcacagt
      481 ttggtttccg gtgagcggca cctgaaagga ctggccggca gccagcgcaa gtgctgccca
      541 aaaaaccgcg cccatcgcgc acaatggcac gagaatccgt cgaggtacgc tcatcattgg
      601 tttactccac cggaagatca cggcctgata aggccgcacc cgcggattgt atgcacggtt
      661 gatctcgggg gcgagactcg gcctgttagg aggcgtagcc atggctctag tgcagaggtt
      721 caccgggata ttcgcgctcg cgctcagcgt ggccgctggc ggagcagctc catcggatgc
      781 cacggtccac caactcatga cccgcgatct catcggcatg cccggcaagg aggtaatgat
      841 ggagacggtg gtctacgccc cgggaggcaa gtcaccgcct caccgacaca gcgctcaggt
      901 attcgtctat ggcctcgagg gatcgctgcg catgcaggtg cagggctcgc ccgtcgtcat
      961 cgtcggcccc ggagatacct tctacgaggg tccggacgat gtccacgtcg tctcggagaa
     1021 cgcgagccag acgcaacccg cgaagttcct ggtcgtgatg ctgaaggaca agccgaccgg
     1081 cagtcatgag tgaagcccct gcggctgtgc cccaaggccc cgagatcgaa atcatagata
     1141 tcgggccgct gttcgatacc gatccgactg cgtggaggct tcctgaccgc gcgctgctag
     1201 cggcggcgtg cgatatcggc tttgtctgtg ttcgtggtct gccgggggat cgggcgcttg
     1261 caccagaccg gcgagcacag ctcctgagga tatttgccct cgacgatgcc gccaaacggc
     1321 gcctgtttcg gcggaagttc gcacccggga accgcaacgt ctaccgcggc tggtttcctt
     1381 tgcagcctgg aaatctgact tacaaggaag gcatcgacat cggcggcgac gtcgcgcacg
     1441 ggtcagccat ctgcgtgccc ggcgacccgc tgcgcgagcc gagtccgcta cccgaggatg
     1501 accgccttcc gggttggcgc gcagtggtcg cagcgtatta ccgcgcgatg gagcgcgttg
     1561 ccgaggcgtt gatgcgctcg atcgcacgca gcctgcaact cccgcagggc tatttcgatg
     1621 acgcgtttcg ccagggtcta tcgaccttac ggctgattcg ctacccgccg cgcgcggcag
     1681 ccgagctggc aacggtgacg gcccctgccc tgtggatcga ggcagatgct acccgccagc
     1741 atctcatcgg cgcaccgcat acggactcgg gcttcatcac gctgctcgcg caggacggtg
     1801 ccggcggact gcaggcgcgc tcacgggatg gacgttggat cgatgtaccg ccgctcgccg
     1861 acacgctggt cgtcaatttc ggtcaggtgc tcgagcagtg gtccgccggc cgaattcgtg
     1921 ccaccgagca ccgcgtgctc ggccgcggca tcgagcgtta ctccataggg ttcttctacg
     1981 aagcgcgcgc cgacgcaacc atcgcacctc tgccggtcga ttcgcctgac tccttcgtgc
     2041 cgtttcaata tggcgacttc ttgtggaggc gcatcacctc atttgtcgag ttccgcggca
     2101 tggagggaga gcgcgcccag tcgagccggt gatatgagcc tgtacggtat cgctgccctg
     2161 cccgctctct tctggttgta cctcctgcta ggccgcgggc agttctggcg cgtggccgca
     2221 cagcgcgtgc cgcagctccc gccggccgcc gagcggcaca tcgttgcgct cataccggcc
     2281 cgcaatgagg cacccgtgat agggatggcg gtggcatccc tcgcgcgcca ggtgttcaac
     2341 ggtcatatcc atatcatcgt gatcgacgat ggcagcagcg atggcaccgc ggaagcggcg
     2401 ctggacgcag cgcgtaccgc gggcgcgctc gcgcgcttta cgttcctgcg cagtgacaag
     2461 ctgccgcacg gctggaccgg caagttgtgg gctctttccc agggcgtagc ggccgccacc
     2521 acgctcgaac ccgattatct gcttttctcg gatgccgaca tctgtcatgg caccgtgagc
     2581 gtcgcctcgt tggttgcaga cgccgaggcc aacgaccgcg atctggtatc gcacatggtg
     2641 aagttgtcga ccgttaccct cgcagagcga ctgctgattc cggcattcgt gtttttcttc
     2701 ttcaagctct atccacctgc ctggatcgcc gattcacgtc gcaagctggc agcggcggcc
     2761 ggtggttgca tgctgatccg gcccgcggcg cttgcgcgcg ccggcgggct gccggccatt
     2821 cgctcgcaca tcatcgatga ctgtgcactg gcacgcgcga tcaaggcttg cggtggccgc
     2881 attgcgctgg agctcgccgc cgacactagc agtctgcggc gctacggatc ctgggcggag
     2941 atcggcgcaa tgatttcccg ctcggcattt gcccagctcc accattccta tctgctggtc
     3001 gcagcgacgc tggttggaat gttcctgact tacctgctcc ccgtctggtt gctgttcgcc
     3061 ggtaatccgc tgctcgtcgg cttgggtatc accgcttggc tactcatgag cctttgttac
     3121 ctgccgatgg tgcgctttta cggtcaggcg ccgctctgga gcctgtgctt gccgttcact
     3181 gccattttct attccggtgc ggtgatccac tctgccgttc aacacgcccg cggacgtggg
     3241 ggtgtgtgga agggacgcgt gcaagatgcg tgaatcgctg gtgcgttcgt gatatacgag
     3301 accgtcctga agtcggccca tgagtccgat ctcgatgaac aactgcggac gcaaagtcag
     3361 attcgctgct ctacacacgc tcgtaacttg cctgctggcg ttagcagtca gcgcatgcgc
     3421 cggcgagaag ccaacccgcg agcaagcgat agagcgctac agccaggagc tacgccaggc
     3481 ggtttcgaca
//
LOCUS       DATDMP010000691         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_886312, whole genome shotgun sequence.
ACCESSION   DATDMP010000691 DATDMP010000000
VERSION     DATDMP010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886312"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VI195_12070"
     CDS             complement(<1..630)
                     /locus_tag="VI195_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6825172.1"
                     /translation="MNATVALLEPENAAGPCAEELARINESLQARERLLTASARASRL
                     LLEAPDVRAAVPDVLGLIGAAARVDRVNLMLGYTGPNGERLLSVVSEWTAEHGTVSPP
                     PTPVCTCNEANCAALFAELRAGRSVCFSNGESPVGRACSAIEGVGTQSKAIVPIFVAG
                     EFIGMVGFDNTHGQRAIDAAELAALETAAGVIGAALHRERLVDDVRRERE"
     gene            complement(821..>2065)
                     /locus_tag="VI195_12075"
     CDS             complement(821..>2065)
                     /locus_tag="VI195_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6825173.1"
                     /translation="LIDQALGANHDLRIALGHLMEARAQRHEAQFDLAPTVTASGGYT
                     KQRFPAVDSPTGEPLTQRFYDAGFDAFWELDFFGGVRRNIEARSAQMQGAEAGLRDAQ
                     VSVTAEVARSYFELRGEQARFAVAWRNVENQRATLQVTQARLDAGRSTELDTARAQAQ
                     LSATLSTLGPLEAAIARSIHRLSVLTGQQPDALRELLTPTRELPELPQLTAIGDPAAL
                     LRRRPDIRVAERELAASTALVGVAISDLFPKVTFTGNFSYAAAEPGQLGSSASRGYTI
                     APGITWAAFDLGRVRAEVAGSRARADVALAGYEQTVLRALEETENALITHARTRDSLG
                     DAAAAAAASQTAGRIARTRYEGGMVDFLDVLDAERTQLADEDRLAQSRTDAATSLVAV
                     YKALGGGWQGTPLPRYVRQAGG"
BASE COUNT          321 a          701 c          731 g          312 t
ORIGIN      
        1 ctcgcgctcg cggcgcacgt catcgacgag ccgctcgcgg tgcagcgcgg cgccgatcac
       61 cccggccgcg gtctcgagtg ccgcgagctc ggcggcgtcg atggcgcgct ggccgtgcgt
      121 attgtcgaag cccaccatgc cgatgaactc tccggccacg aagatcggca cgattgcctt
      181 gctctgggtg ccgacaccct cgatggccga gcaggcgcgg cccacggggc tttcgccatt
      241 gctgaaacaa acactgcgcc cggcgcgtag ctcggcgaac aaggcagcgc agttcgcctc
      301 gttgcaggtg cacacgggcg tcggcggcgg tgaaaccgta ccgtgctcgg cggtccactc
      361 gctcaccacg ctgagcagcc gctcgccgtt agggccggta taacccaaca tgagattcac
      421 gcgatcgacg cgcgcggccg cgccgatcaa accgagcaca tcggggacgg ccgcgcgcac
      481 atcgggcgct tccagcagca agcggctggc gcgcgctgag gcggtgagca accgttcccg
      541 cgcctgcagc gattcattga tgcgggcgag ctcctcagcg caagggccgg ctgcgttttc
      601 gggttccaac agggcgacgg tggcattcat gtcagcaggc gctttccccg cgtgcggcag
      661 gccgcaaagt ctaccgcgtc ggacccctgc acggtccgca ttcggcacgg gctctatctt
      721 aagagggagc gccgctccag ccgcccggga agcggtaggg gcggccttgg gcaactgtgg
      781 cttctgcctg gaacgcgtgc gccttccgtg aatcaacggc ttagcctccg gcctggcgaa
      841 cataacgcgg cagcggcgtg ccctgccaac ctccgccgag cgccttgtac accgcgacga
      901 ggctcgtggc cgcatcggtg cgcgactgcg ccaggcgatc ctcgtcggca agctgcgtgc
      961 gctcggcatc gagtacatcg aggaagtcca ccatgccacc ctcgtagcgc gtgcgcgcaa
     1021 tgcgccccgc ggtctgactg gcggctgcgg ccgcagcggc atcgccgaga ctgtcacgcg
     1081 tacgtgcatg ggtgatgagc gcattctcag tctcttcgag tgcgcgcagg accgtctgct
     1141 catagccggc gagggccaca tcggcccgag cgcggctgcc ggcgacctcg gcgcgcacgc
     1201 ggccgagatc gaaagccgcc caggtaatgc cgggcgcgat ggtgtagccg cgactggcgg
     1261 agctaccgag ttgcccgggc tctgcggcgg cgtagctgaa attgccggtg aaggtcacct
     1321 tggggaagag gtccgagatc gcgacaccga ccagcgcggt ggaggccgcc agctcgcgct
     1381 cggcgacgcg aatgtcggga cggcggcgca gcagggcggc tggatcacca atggcggtga
     1441 gctgcggcag ctcgggtaac tcgcgcgtcg gagtgagcag ctcccgcaac gcatcaggtt
     1501 gctgcccggt gagcacactc aagcggtgga tcgagcgggc gatcgcggcc tcgagcggcc
     1561 cgagagtgct cagcgtggcg gaaagttgcg cctgggcacg cgcagtgtcg agctcggtgc
     1621 tgcgcccggc atcgagccgc gcctgcgtca cctgcagcgt cgcacgctgg ttctccacgt
     1681 tgcgccacgc gaccgcgaag cgtgcctgct cgccgcgcag ctcgaagtag ctgcgcgcca
     1741 cctcggcggt gaccgagacc tgggcatcgc gcagcccggc ttccgcgccc tgcatctggg
     1801 cgctgcgggc ctcgatgtta cggcgcaccc cgccgaaaaa atcgagctcc cagaaggcat
     1861 cgaagccggc gtcgtagaag cgctgggtaa gcggctcgcc ggtcgggctg tcgaccgcgg
     1921 ggaacctctg cttggtgtaa ccgcccgatg cggtgacggt cggggcgagg tcgaactgcg
     1981 cttcgtgccg ctgggcacgc gcctccatga gatgaccgag ggcgatgcgc aggtcgtgat
     2041 tggcgccgag cgcctgatcg atcag
//
LOCUS       DATDMP010000692         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_219761, whole genome shotgun sequence.
ACCESSION   DATDMP010000692 DATDMP010000000
VERSION     DATDMP010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219761"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VI195_12080"
     CDS             <1..1124
                     /locus_tag="VI195_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6825174.1"
                     /translation="RRRFPVLERKTYLNSGSYCALADSVRDAIDAYMEDRLLVGANWD
                     VWVTKNEAVRAAMAEVLRAHPDEIAVTASASAGINALASAHDFSGRRNKVVVSDFEFP
                     TNAQIWHAQETRGARVVHVPRAPDGYIPLENFEKVIDEETQLVAVTHVCFRNGAMLDI
                     EGIARLAHARGAKIMLDCYQAVGAVTVDVKRLDVDFAVGGMLKYLLGTAGLGFLYVRG
                     ELIPSLLPSNSGWFAQEDIAAMDITANRPSPTARRFEAGTPAVVNCYAVEAGLKIINE
                     VGTDSIERRIRELTQLCMERLEAIGWASVTPRPEERHGPMICVRAKQVAHLFERLMQR
                     DIVTSFRDDNLRASFHFYNSEDDVESLVAALAALRAEFR"
     gene            1228..>2075
                     /locus_tag="VI195_12085"
     CDS             1228..>2075
                     /locus_tag="VI195_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEY6825175.1"
                     /translation="MNAPHTDARIAGQGSAGSREFPVEALRTQFAALQQAGSFIFFDN
                     AAGAQVPQLVLDAVNHHLVDHNVQRGGRYPQSLAVDATIARSRASVAALVNARRAEEV
                     AFGLNATSFVRLISLAIGQTLGTRNEIIVTDLDHEANIATWLALERMGAKFRWWKMRA
                     GGSLHVEDLVPLLSAHTRLVACTLTSNALGSITDARAAAEAAHAAGAEIFLDSVHYAP
                     HGSIDVQALDCDYLVCSGYKIFAPHMGFLWGRYELLEALPTFREDFIPDAPPGKIEAG
                     TFAFENV"
BASE COUNT          362 a          656 c          684 g          373 t
ORIGIN      
        1 tgcgcaggcg ctttcctgtg ctcgagcgca agacctacct gaacagcggt tcgtattgcg
       61 ccttggccga cagtgtgaga gacgccatcg acgcttacat ggaggatcgg ctgctggtcg
      121 gagcgaactg ggacgtgtgg gtcactaaga acgaagcggt gcgagcggca atggctgagg
      181 tgctgcgcgc gcaccccgat gagatcgccg tgaccgcctc ggcatccgca ggcatcaatg
      241 cactcgccag tgctcacgac ttttcggggc gccgcaacaa agtagtcgtg agcgacttcg
      301 agttcccgac caacgcgcag atctggcatg cgcaggagac gcgtggtgcc cgggtggtgc
      361 atgtgccgcg ggcgcccgat ggctacattc cactggagaa tttcgagaag gtcattgatg
      421 aggagacgca actcgttgcc gtcacccacg tttgtttccg caacggagcg atgctcgaca
      481 tcgaaggaat cgcccgcctc gcgcacgcac gcggggcgaa gatcatgctc gactgctacc
      541 aggcggtcgg cgcggtcacg gtggatgtga agcgcctcga tgtcgacttc gccgtgggcg
      601 ggatgctcaa gtacctgctg ggaaccgcgg gactcggatt cctctacgtg cgaggcgagc
      661 tgatcccgag cttgctcccc agcaattccg gctggttcgc ccaggaggac atcgcggcga
      721 tggacatcac ggccaaccgt ccatcgccga cggcgcggcg cttcgaggcg ggcacgccgg
      781 ccgtggtcaa ctgctacgcg gtcgaagcag gcctcaagat catcaacgag gtgggcaccg
      841 actcgatcga gcggcggatc cgggagctga cgcagctgtg catggagcgt ctggaggcca
      901 ttggctgggc gagcgtgacg ccgcgccccg aggaacgtca cgggccgatg atctgcgtgc
      961 gcgcgaagca ggtcgcgcac ctgttcgaga ggctcatgca gcgggatatc gtcacttcgt
     1021 tccgcgacga taacctgcgc gcgtcctttc acttttataa ctcggaggat gacgtcgagt
     1081 ccttggtcgc ggctcttgca gcgctccgtg ccgagttccg ctagcgcttt ctagtacccg
     1141 cggcctaatt ttggcgcgcc gaggcaggcc ggtggtccca ttatcattca gacaaagtgg
     1201 tacggcagga tcaccgcgga gcgccacatg aatgcaccgc acacggatgc acggatagcg
     1261 gggcagggca gcgccggctc gcgcgagttt cccgtggagg cgttgcgcac ccagttcgcc
     1321 gccctacagc aagccggttc cttcatattt ttcgacaatg ccgccggcgc ccaagtgccg
     1381 cagctggtgc tcgatgccgt gaatcaccat ctggtggacc acaatgtgca gcgcggcggc
     1441 cgctatccgc aaagtctcgc ggtggacgcc acgattgcgc gttcgcgtgc ctcggtggcg
     1501 gcgctggtga acgcgcggcg cgccgaggaa gtcgccttcg ggctgaatgc aacctcattc
     1561 gtacgactga taagtctcgc gatcgggcag acgctcggca cgcgcaacga gatcatcgtc
     1621 accgacctcg atcacgaggc gaacatcgcg acctggctcg cgctggagcg catgggcgca
     1681 aagtttcgct ggtggaaaat gcgcgccggg gggtctctgc acgtcgagga tctcgtgccg
     1741 ctgctgtcgg cgcacacgcg cctggtcgcc tgcacgctca cgtccaatgc gctcgggtcg
     1801 atcaccgatg cgcgtgcggc ggcggaggcg gcgcacgccg ccggcgccga gatctttctc
     1861 gacagcgtgc actatgcccc ccacggatcc atcgacgtgc aggccctcga ctgcgattac
     1921 ctggtctgct ccggatacaa gatcttcgcc ccgcacatgg gcttcctctg gggccggtat
     1981 gaattgctgg aggcgctgcc gaccttccgc gaggacttca ttcccgatgc gccgcccggc
     2041 aagatcgagg caggcacctt cgccttcgag aacgt
//
LOCUS       DATDMP010000693         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_109880, whole genome shotgun sequence.
ACCESSION   DATDMP010000693 DATDMP010000000
VERSION     DATDMP010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109880"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VI195_12090"
     CDS             <1..370
                     /locus_tag="VI195_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6825176.1"
                     /translation="QEEIARRGWARTVEESLALLEQPQVVLIDLRERSERERHGAIPG
                     SLHVPYPTLEESTQPGGMLHALARSSGKRLLFYCAFGERSAMAVQCAQDAGLSSACHL
                     QGGLAAWKAAGAPIESCQKR"
     gene            432..1274
                     /locus_tag="VI195_12095"
     CDS             432..1274
                     /locus_tag="VI195_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825177.1"
                     /translation="MTSALPESSAHLLREQQDFSLVHGGPLFQLLVRSHLASNALTLV
                     QRRVIVFVLLTWVPLLALSAVTGRLLDRSVAVPFLLDLETHTRFLVVVPLLLAAELIV
                     HRRLLRVVRSFLDRNMIPEAEMPRFDAAVASAFRLRNSVTAEVLLLLLVYVIGVLIVW
                     RRFVAIDVSTWYATASVPGSKLTLPGLWYAYVSLPIFQFLLLRWYFRLLIWIRFLWQA
                     SRVDLKLVPTHPDQIGGLGFLSHTVFGFSVLLMAHGAMLAAQIANRIFFTGASFSSSR
                     SRLS"
     gene            1274..1642
                     /locus_tag="VI195_12100"
     CDS             1274..1642
                     /locus_tag="VI195_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825178.1"
                     /translation="MLAFLMCIVFGPLLVFSPRLARAKRMGLLEYGTLAERYVREFDS
                     KWLRGRSQQSEPLMGSADIQSLADLGNSFSMVRNMRIAPITRDAVLQLAGCVLVPLTP
                     LLLTVMPVEELTKKLLGLVF"
     gene            complement(1714..>2484)
                     /locus_tag="VI195_12105"
     CDS             complement(1714..>2484)
                     /locus_tag="VI195_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY6825179.1"
                     /translation="ITLYNRLRNEPRLQAAPRCCIFGGKAAPGYTMAKLIIRLINGVA
                     EVVNNDAAVGARLKVVFLPNFNVKSSHTIYPAADLSEQISTAGKEASGTGNMKFMLNG
                     ALTIGTLDGANVEMREEAGAENFFLFGLTVEQVEALRREGYQPARELERNPELRAALE
                     LIASGMFSHGDRELFRPLLENLRNSDPFLVLADYADYLACQQRVSAAWQDVGHWTRMS
                     ILNTARAGKFSSDRAVREYCDEIWGAKPVRTALDPDLS"
BASE COUNT          397 a          794 c          790 g          503 t
ORIGIN      
        1 ccaggaggag atcgcgcggc gcggctgggc acggaccgta gaggagagcc tcgcgttgct
       61 ggagcaaccg caggtcgtgc tgatcgacct gcgcgagcgc tccgagcgcg aacgtcatgg
      121 agccattccc ggctcgctgc acgtgccgta tccgacgctg gaggagagca cgcagccggg
      181 cgggatgctg cacgcgctcg cccggtccag cggcaagcgg ctgctgttct actgcgcatt
      241 cggtgagcgc tcggccatgg cggtgcagtg cgcgcaggat gccggactct cctccgcctg
      301 ccacctgcag ggtggcctcg ccgcctggaa agcggctggc gctccgatag aatcgtgtca
      361 gaaacgataa ctgggacgag cccacgctcg caaaaaccgc gctcgcgtcg gcgccgagtt
      421 cgataaccag aatgacatcc gcactacctg aatcgagcgc gcacctgctg cgcgaacagc
      481 aggacttttc cctggtgcac ggcggtccgt tgttccagtt gctggttcgt tcgcaccttg
      541 ccagtaacgc gctcactctg gtgcagcggc gcgtcatcgt gttcgtgctg cttacctggg
      601 tgccgctgct cgcgttgtcc gcagtaacag gccgactgct cgacagaagt gtcgccgtgc
      661 cgtttctgct ggatctggag acacacaccc gctttctcgt ggtggttccg ctgctactcg
      721 ccgcggagct catcgtgcac cgtcggctgc tgcgcgtcgt gcgatccttc ctcgatcgaa
      781 acatgatccc ggaagccgaa atgccgcgct tcgatgcagc cgtcgcctcc gcatttcgtc
      841 tgcgcaactc cgtgacggcg gaggtgctgc tgctgctctt ggtatacgtg atcggtgtgc
      901 tcatcgtatg gcgtcgcttc gtcgccatcg atgtatcgac ctggtacgcg accgcttcgg
      961 tcccgggctc gaagctcacc ctacccggcc tgtggtacgc ttatgtgagc ctgccgattt
     1021 ttcagtttct gctgttgcgc tggtactttc gcctgctcat ctggatacgt ttcctgtggc
     1081 aggcgtcgcg cgtcgacctc aaactcgttc caacacatcc cgaccagatc ggtggtctgg
     1141 gctttctgag ccacactgtc ttcggattca gcgtgctgct gatggcgcat ggcgctatgc
     1201 tcgctgcgca gatcgccaac cgcatattct tcaccggcgc aagcttcagc agttcaagat
     1261 cgagattgtc gtgatgctcg ccttcctgat gtgcatcgtc ttcggtccat tgctggtgtt
     1321 ttcgcccagg ctcgcacggg caaagcggat ggggctgctc gagtacggca ccttggccga
     1381 gcgctacgtg cgtgagttcg acagcaagtg gctgcgcggc cggtcgcagc agagcgagcc
     1441 cctgatgggg agcgccgata tccagtcgct cgccgacctg ggcaatagct tctccatggt
     1501 acggaacatg aggattgcac cgatcacgcg cgacgctgtc ctgcaactgg ccgggtgcgt
     1561 gctcgtaccg ctcactccct tgctgctgac cgtgatgccc gtcgaggagt tgacgaaaaa
     1621 gctgctcggt ctggtcttct gagcctcgac gatgtcgcgc aatgtatccg gcggatagcg
     1681 gctgccgtcg cgggtgggca gtgcggcggg cactcagctc aggtccggat cgagcgcggt
     1741 cctcaccggc ttggcacccc agatctcgtc gcagtactcg cgcaccgccc ggtcggagga
     1801 aaacttcccg gcgcgcgccg tgttgaggat agacatgcgc gtccagtgcc cgacatcctg
     1861 ccaggcggcg ctgacgcgct gctggcacgc caggtaatcg gcatagtcag ccagcaccag
     1921 gaagggatcg gagttccgca ggttttcgag cagcgggcga aagagctcgc gatcaccgtg
     1981 tgagaacatg ccggaggcga tcagctcgag cgccgcgcgc agctcgggat tgcgttcgag
     2041 ctcgcgcgcc ggctgataac cttcccgccg gagcgcttcg acctgctcca ccgtcaggcc
     2101 gaacaggaag aaattctccg cgccggcctc ttcgcgcatc tcgacgttcg caccgtcgag
     2161 ggtgccgatg gtcagcgccc cgttcagcat gaacttcatg ttcccggtac cggaggcctc
     2221 cttgccggcc gtggaaatct gctccgacag gtcggccgcc ggatagatgg tgtgcgagct
     2281 cttcacgttg aaattgggca agaaaacgac tttcaggcga gcgccgactg ccgcatcgtt
     2341 gttcacgacc tcggccacgc cgttgatcag acggatgatg agcttcgcca tggtgtagcc
     2401 gggtgccgcc ttgccgccga agatgcagca gcgcggcgcc gcctgcagcc gcggctcgtt
     2461 tcgcaggcgg ttgtagagcg tgat
//
LOCUS       DATDMP010000694         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1443013, whole genome shotgun sequence.
ACCESSION   DATDMP010000694 DATDMP010000000
VERSION     DATDMP010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443013"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VI195_12110"
     CDS             <1..492
                     /locus_tag="VI195_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY6825180.1"
                     /translation="NGRFTPAQRAVYQIVLEANRAAIACVRPGNHWNAPHEAAVRVIT
                     QGLVKLGLLKGRLPALIKSGAYRRFFMHRTGHWLGLDVHDVGDYKVGDEWRVLEPGMV
                     MTVEPGIYLPAGARGVPRRFRNIGIRIEDDVVVTKRGAEVLTARIPKDPDQIEALMAR
                     RAA"
     gene            496..1737
                     /gene="ubiH"
                     /locus_tag="VI195_12115"
                     /gene_synonym="visB"
     CDS             496..1737
                     /gene="ubiH"
                     /locus_tag="VI195_12115"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEY6825181.1"
                     /translation="MCAAAARQLEVDVAIVGGGMVGASLAVALAGTGRKLLLIESIPF
                     GNAAQPSFDERTTALGNASRRIFAGLGVWAGIAPEAAAIRTIHVSDAGRFGFARLSAA
                     EQGIDAFGYVVANRCIGAALWRALSAAPELSLEVPARVEDLAPTAECVRFTVVSESAP
                     AMAVRSRLLVAADGAHSQVRAATGIAADVVDYRQVAVVANVGTDRPHEGQAFERFTPA
                     GPLAVLPRHDGGVAVIWACRPQHAQELMALDEQSYLAQLQAQFGWRAGRFVRAGQRSC
                     YPLKLTRAMAGTGVRTVLIGNAAQSLHPVAGQGFNLGLRDAAMLAEVIAGTSGDVGAP
                     SVLSRFLEWRARDRRGVVGFTDGLIRVFGDPRAGTALLRNLGLLVFDLAPPAKEALAR
                     VSAGFGGPTPRLARGLPLRHV"
     gene            1730..>2000
                     /locus_tag="VI195_12120"
     CDS             1730..>2000
                     /locus_tag="VI195_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY6825182.1"
                     /translation="MFDVVIVGGGPVGACAGALLAHGTPGVAGLSVALLEPRPPGAPP
                     ADARPDPRVVALSRASERLLRRAGAWTRIAPERLSAYERMRIWHES"
BASE COUNT          265 a          666 c          733 g          336 t
ORIGIN      
        1 aacggtcgtt tcacgccggc gcagcgcgcg gtttaccaga tcgtactgga ggccaaccgc
       61 gccgccatcg cctgcgtgcg tccgggaaat cactggaacg cgccgcacga ggccgccgtg
      121 cgggtgatca cgcagggact cgtgaagctc ggcctgctga aaggccggct gcccgcgctg
      181 atcaagagcg gcgcgtaccg gcgcttcttc atgcaccgca ccggtcattg gctcgggctc
      241 gatgtccacg atgtgggcga ctacaaggtc ggcgatgagt ggcgcgtgct cgagccgggc
      301 atggtgatga ccgtcgagcc tggcatctat ctgccggcag gcgcgcgcgg ggtgccacgc
      361 cgctttcgca acatcggtat tcgcatcgag gacgacgtgg tggtcaccaa gcgtggggcg
      421 gaggtgctca ccgcgcgcat tcccaaggac cccgatcaga tcgaagccct catggcgcgc
      481 cgcgccgcgt gaaccatgtg tgccgcggcg gctcgtcagc tcgaggtgga tgttgccatc
      541 gtcggtggcg gcatggtggg agcgagcctc gccgttgcgc tcgcagggac cgggcgcaag
      601 ctgctcctca ttgagagcat ccccttcggc aatgccgcgc agccgagctt cgatgagcgc
      661 acgaccgcgc ttggcaacgc cagccggcgc atttttgcag gcctgggcgt gtgggcgggc
      721 atcgcgcccg aggcagcggc gatccgcacg atccatgtct ccgatgcggg acgcttcggc
      781 ttcgcgcgct tgagcgccgc ggagcagggg atcgacgcct tcggatacgt tgtggcgaat
      841 cgctgcatcg gcgcggcgct ctggcgtgcg ctgagcgcgg caccggagct gagcctcgag
      901 gtccccgcgc gggtcgagga cctcgcgccg accgccgaat gcgtgcgttt cacggtggtg
      961 agcgagtcgg cacctgccat ggcggtgcgc agccggctgc tggtcgccgc cgatggtgcg
     1021 cactcacagg ttcgcgctgc caccggcatc gcggccgatg tggtggatta tcggcaggtg
     1081 gcggtggtgg cgaatgtcgg caccgatcgt ccccacgagg gtcaggcatt cgagcgcttc
     1141 actcctgccg gcccgctcgc cgtgctgccg cggcacgacg gcggtgtcgc ggtcatctgg
     1201 gcctgccggc cgcagcatgc acaggagctg atggcgctcg atgagcagtc gtatctggcg
     1261 caactgcagg cgcagttcgg ctggcgtgcc ggacgattcg tgcgcgcggg gcagcgcagc
     1321 tgctatccgc tgaagctcac gcgtgccatg gcgggaaccg gggtgcgcac cgtactcatt
     1381 ggtaacgctg cccagtcgct gcaccccgtc gccggtcagg gcttcaatct ggggctgcgc
     1441 gatgccgcga tgctcgccga agtgatcgcc ggtacgagcg gcgatgtggg ggcaccctcg
     1501 gtcctctctc gcttcctcga gtggcgcgcg cgcgatcgcc gcggagtggt cggtttcacc
     1561 gacggactga tccgcgtctt tggtgatccg cgcgcaggca ctgcgctgct gcgcaacctc
     1621 gggctgctcg tgttcgatct cgcgcccccc gccaaggagg cgctggcgcg cgtcagcgcg
     1681 ggcttcggcg gaccgacacc gcgcctcgcg cgtggactgc cactgcgtca tgtttgacgt
     1741 cgtcatcgta ggcgggggcc ctgtcggagc gtgtgccggc gcattgctcg cgcacggcac
     1801 accaggggtt gcgggactct cggtggcgct gctcgagccc cgccctcccg gcgcgccgcc
     1861 cgcggatgcg cgcccggatc cgcgcgtggt cgcgctgtcg cgtgccagcg agcggctgct
     1921 gaggcgcgcc ggtgcctgga cgcggatagc acccgagcgg ttgtcggcgt acgagcgcat
     1981 gcgtatatgg cacgagagca
//
LOCUS       DATDMP010000695         4693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1443200, whole genome shotgun sequence.
ACCESSION   DATDMP010000695 DATDMP010000000
VERSION     DATDMP010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443200"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VI195_12125"
     CDS             complement(<1..819)
                     /locus_tag="VI195_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825183.1"
                     /translation="MTLRARMLAALLLASVGVAAEPPEPPQKSERDAAPVELSLTLSQ
                     QQAVGIRTEYPLPLATAPALEAYGTVLDPLTLATDAGRMESTQAAAAAATAEAARQQR
                     LYREEAQASLKASQASQAQAVEAAAQASAAAMTFRLQWGPLAHWSAAQRRALLEELSG
                     GRQRLLRAELPGQHVVSALEPAALVEIDGVNLTARVLGALPRTDAQSQSAAWLLLLEH
                     APEGLGPGAPALVRLRSAAPTRGVLVPAAALLYAEDGTYVYRQVHARGNADKFVY"
     gene            complement(816..2222)
                     /locus_tag="VI195_12130"
     CDS             complement(816..2222)
                     /locus_tag="VI195_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6825184.1"
                     /translation="MTSSAARTVLALAAVIALADCGSTPGRAPLEPAATAAEYSTRRL
                     DSLSPELPPPSSGWDRSQWLAAALQLNPRLAEQRAEVDAVAAGERTAAERPNPSMQLF
                     AEYLSSAAQSTAWLYGLSMDFLLRQPGERGRAQRRAALQTALAKSDLAESLWQVRAAL
                     RQALLDAVSAQDESALLQALVAQRRALLDADRARLQAGDIARAQVLADELELSHAQQR
                     QQQSRIRSADALARLASAVGVPVAALEAVPLRWSGWDQIDSLSVDSASRWRTEALIGR
                     PQVIHALREYDLAEVGLQSEVAKRWPQIHVTPAYAWGGEGVRQNTLYDIATEAGVALS
                     FEVPIFNQHQGPIGEAVARRTAAGEHLKSVQAEIFGEIDRAELAWPPAQQAWADTRRA
                     AELAQHQRQAEQHALAAGEVERASVLSAEITATEAQLSVLQSAYTAETVFGALEDAYR
                     RPLQGNEGQWPPAASPQS"
     gene            complement(2306..3166)
                     /gene="fghA"
                     /locus_tag="VI195_12135"
     CDS             complement(2306..3166)
                     /gene="fghA"
                     /locus_tag="VI195_12135"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY6825185.1"
                     /translation="MLETISEHRCFDGVQGFYRHQSAVIGLPMRFAVFQPPQARQQRV
                     PVLFYLAGLTCTEETFAIKAGAQRVAAEEGLMLVSCDTSPRQTGIPGEADDWEVGTGA
                     GFYLNATQPPWSRYFRMYDYVLGELRETVLTQFPAVAARVGIFGHSMGGHGALVLALR
                     NPEVYRSVSAFAPICAPSRCPWGRKAFTRYLGEERAAWARYDAVELLDAQRARVFPEI
                     LVDQGLADKFLPQGQLLPELLEQACAQAGQPLRLRRQDAYGHDYYFIATFMEDHLRFH
                     AQALAAVAGS"
     gene            3564..>4693
                     /locus_tag="VI195_12140"
     CDS             3564..>4693
                     /locus_tag="VI195_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6825186.1"
                     /translation="MSMRQSLVRLLSSAIAAAALLPTARAADLGALVGRVTTAAGAPV
                     AYATVTATRADGGAIRATLSGSDGIYSFGDLPPGTWTVSVQGAGLPSVSAPSLTVVTG
                     KATRADLVMNIAPQPAAPAAPAVAAAVPEALQKPEPAPEVDTQTPFAVGDLGWMNGTS
                     RATTPVFDTKFFTPEVRFDVNYLQSFNHPNDHTIVGSTEEFRSGEFQIEQVSLGGNFH
                     WHNVQARFLSLFGMFAVTTPRNDASPAVGQWDLQGAYKYFSEANAGYHWDVDHGLNVD
                     AGIFVSYVGLFSYYNFDNWTYQPSFVSSNTPWFFNGLRVQWFPTQTLKIEPWLINGWQ
                     SYAKFNSHPGFGGQILWIPNDSLKLVFNTYTLGQDNLASGSGV"
BASE COUNT          753 a         1636 c         1572 g          732 t
ORIGIN      
        1 gtacacgaac ttgtcggcgt tgccgcgcgc gtgcacctgg cgatagacgt aggtgccgtc
       61 ctcggcgtac agcagagcgg cagcgggcac cagcacaccg cgcgtcggcg ccgccgaacg
      121 cagccttacg agcgcggggg cgccaggccc gagaccctcc ggcgcgtgct cgagcagcag
      181 cagccaggcg gcgctttgtg actgcgcatc ggtgcgcggc aatgcgccga gaacgcgtgc
      241 ggtgagattg acgccgtcga tctccaccag ggcagcgggc tcgagcgcgc tcaccacatg
      301 ctgccccggc agctcggcgc gcagcagccg ctgacggccg ccggagagtt cctcgagcag
      361 tgcgcgccgc tgcgcggcgc tccagtgcgc gagcggcccc cactgcaatc tgaaagtcat
      421 ggccgccgcg ctcgcctggg cggccgcttc gaccgcctga gcctgcgagg cctgcgaggc
      481 tttcagcgag gcttgggcct cttcgcggta aagacgctgc tggcgtgcgg cttcagcggt
      541 cgccgcggcg gctgcagcct gcgtgctctc catccggccc gcatcggtcg caagcgtgag
      601 cggatcgagc accgtgccat aggcctcgag cgccggcgcc gttgcgagcg gcagcgggta
      661 ctcggtgcgg atcccgacgg cctgctgctg tgacagtgtc agtgacagct cgacgggcgc
      721 cgcgtcgcgc tccgacttct gcggcggctc cggcggctcc gcagccacgc cgaccgaggc
      781 gagcagcagc gccgccaaca tcctggcgcg caaggtcatg attgtggaga cgcagcaggc
      841 ggccactggc cctcattgcc ctgcagaggt cgacggtagg cgtcttcgag cgcgccgaag
      901 accgtctcgg cggtgtaggc cgactgcagc acggacagtt gcgcctcggt ggcggtaatc
      961 tcggcgctca acacgctggc gcgctccacc tcgcctgcgg cgagcgcgtg ttgctcggcc
     1021 tgacgctggt gttgggcgag ctctgcggcg cgacgggtgt cggcccatgc ctgttgcgct
     1081 ggcggccatg ccagctcggc gcggtctatc tcgccgaaga tctccgcctg caccgacttg
     1141 agatgctccc ccgcggcggt gcgccgcgcg acggcttcgc cgatcgggcc ctgatgctga
     1201 ttgaagatgg gtacctcgaa cgagagtgcc acgccagcct cggtggcgat atcgtacaag
     1261 gtgttttgcc gcactccttc gccgccccac gcataagccg gtgtgacgtg gatctgcggc
     1321 cagcgcttgg ccacctcgct ctgcagaccg acttccgcga gatcgtattc gcgcagcgca
     1381 tgaatcacct gcgggcggcc gatgagagcc tcggtgcgcc agcggctcgc ggaatcaacg
     1441 ctcagcgagt cgatctggtc ccagccgctc caacgcagcg gcaccgcctc gagcgccgcc
     1501 accggcaccc ccaccgcgga ggcgagccgg gccagggcat cggcgctgcg tatgcgggac
     1561 tgctgctggc gctgctgtgc gtgcgacaac tccagctcat cggcgagaac ctgcgcacgt
     1621 gcgatatcgc cggcctgcag ccgcgcacga tcggcatcga gcagcgcccg ccgctgtgcc
     1681 accagcgcct gcagcaatgc gctctcgtcc tgagcggaca cggcatcgag cagcgcctgg
     1741 cgcagcgccg cccgcacctg ccagagtgat tcggcgaggt ccgacttggc cagcgcggtc
     1801 tgcagggcgg cgcgccgttg ggcgcgaccg cgctctccgg gctgacgcaa caggaaatcc
     1861 atcgacagcc catacagcca agcggtgctc tgcgcggcac tgctcagata ttcggcaaac
     1921 agctgcatgc tcggattggg ccgctccgcg gcggtacgct cgccggcggc cactgcatcg
     1981 acttccgcgc gttgctcggc gagccgcggg ttgagctgca gtgctgcggc cagccattgg
     2041 gaacggtccc agcccgagga aggtggcggc agctccgggg agaggctatc gagtcgccgg
     2101 gtgctgtatt cggccgccgt cgccgcgggc tcgagcggtg cgcgccccgg agtgctgccg
     2161 caatcggcga gcgcgatgac ggccgccagc gcgagcaccg tgcgagcagc gctcgatgtc
     2221 atcgctcgca acgcgcgtct cgatggttgc acccgccgat actccccgca gcggagtata
     2281 gagccgcggc accccgatcg cagtctcagg accccgccac ggcggcgagc gcctgcgcgt
     2341 gaaagcgcag gtgatcctcc atgaaggtgg cgatgaaata ataatcgtgc ccgtaggcat
     2401 cctgacggcg cagccgcagt ggctgaccgg cctgcgcgca ggcctgctcg agcagctcgg
     2461 gcaacagctg cccctgaggc agaaatttat cagcgagtcc ctggtcgacg agtatttccg
     2521 gaaacacgcg cgcccgctgc gcatcgagaa gctccactgc gtcatacctc gcccaggcgg
     2581 cgcgctcctc gcccagatag cgcgtgaacg ccttgcgtcc ccacggacag cgcgatggcg
     2641 cgcagatggg ggcgaacgcc gagaccgagc ggtaaacctc gggatttctc agtgccagca
     2701 ccagagcgcc atggccgccc atggaatgcc cgaaaatacc cacccgggcg gccacggcgg
     2761 ggaactgcgt cagcaccgtc tcccgcagct cgccgagcac gtagtcatac atgcgaaagt
     2821 aacgcgacca gggcggctgc gtggcgttca ggtagaaacc cgcgcccgtg ccgacttccc
     2881 agtcatcggc ctccccggga attccggtct gccgtggact ggtgtcgcaa ctcaccagca
     2941 tcaggccttc ttcggcggcg acgcgctggg cgccggcctt gatcgcgaag gtctcctcgg
     3001 tgcaggtgag gccggcgagg taaaacagca ccggcactcg ctgctgccga gcctgcggcg
     3061 gttggaagac cgcgaatcgc atcggcaggc cgatcacggc cgactgatgg cgatagaagc
     3121 cctgcacccc atcgaagcat ctgtgctcgc tgatcgtttc gagcatcacg ggtactccac
     3181 gaacgccgcg gcgggggcac attatatgaa tccgcctgcc gagcgggcct gcacccgcag
     3241 ctgtgccaca acgtgcagcg gctgactcaa ccgtgccaga ttggatacgc gcctgtgccg
     3301 cggtgacagc ggcggcgggc gacgctatat tgaggtcctc cgcgcgtccg gcgacatcgc
     3361 gcacgtgccg agcgcgcgga agtcatcgct ttaatcgctg agtgtcgaat agcgggaccg
     3421 tccttccccc ggggcggtcc cgccccagcg ccctgccatc gcagcacgtt cgccgtttct
     3481 taacggcgtt tggtctggtt agacccaggg cgctgcgtac gatggcgcgc tcggacgcac
     3541 ccgtcacaag ccaaggaaaa gccatgagca tgcggcagag cctcgtgcgc ctgctgtcgt
     3601 ctgcgattgc ggccgcggcg ctgctgccca cggcgcgggc cgcggacctc ggtgcgctcg
     3661 tcggccgggt gaccacggcg gcgggtgcgc cggtggcgta cgccacggtg accgcgacgc
     3721 gcgccgacgg cggcgcgatc cgcgcgacgc tctccggaag cgacgggatc tattccttcg
     3781 gcgatctgcc gcccggcacc tggacggtga gtgtgcaggg ggcgggcctg ccgagcgtga
     3841 gcgcaccctc gctcaccgtg gtcacgggca aggcgacgcg cgcggatctg gtgatgaaca
     3901 tcgcgcctca acccgcggcg ccggccgccc cggcggtcgc ggctgccgtg cccgaggcgc
     3961 tgcagaaacc cgagcccgca cccgaggtcg acacgcaaac gccgttcgcg gtgggcgacc
     4021 tcggctggat gaacggcacg agccgcgcga ccacgccggt gttcgacacc aagttcttca
     4081 cgcccgaggt tcgcttcgat gtgaactatc tgcagagctt caatcacccg aacgatcaca
     4141 ccatcgtcgg ctcgaccgag gagttccgct cgggtgagtt ccagatcgag caggtgagcc
     4201 tcggcggcaa ctttcactgg cacaacgtgc aggcgcgctt cctgtcgctg ttcggcatgt
     4261 tcgccgtgac cacaccccgc aacgatgcca gcccggcggt cggccagtgg gatctgcagg
     4321 gcgcctacaa gtacttctcc gaagccaatg ccggctatca ctgggacgtc gatcacgggc
     4381 tcaacgtcga tgccggcatc ttcgtctcct acgtggggct cttcagctac tacaatttcg
     4441 acaactggac ctaccagccc tcgttcgtat cctccaacac gccgtggttc ttcaacgggc
     4501 tgcgcgtgca gtggttcccg acgcaaacgc tcaagatcga gccatggctc atcaacggct
     4561 ggcagtccta tgcgaaattc aacagccatc cgggcttcgg cggacagatc ctgtggatcc
     4621 cgaatgactc actcaagctg gtgttcaaca cctacaccct cggccaggat aatctggcga
     4681 gcggcagcgg cgt
//
LOCUS       DATDMP010000696         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1776281, whole genome shotgun sequence.
ACCESSION   DATDMP010000696 DATDMP010000000
VERSION     DATDMP010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776281"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2275)
                     /locus_tag="VI195_12145"
     CDS             complement(<1..>2275)
                     /locus_tag="VI195_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825187.1"
                     /translation="SVHSALIEVRRRTKPSLPGPPSFLPAWLFINAEDAQIGSAQLTV
                     YNGFQLTVSDIRGAAIIRHSYIRFFGLDGRLEGAHVNALGTLRATDPLGMEVKGHLEW
                     TPAGQPSWTLAGSARGNLDALNVVAHVTSPFRADLSGQALELTSHWHWAADAAVQSFD
                     LRAFHLSSPLGSMTGHLAGSGDEHGFQAHGPIDPTGLHAGVFEVRFDGNYAAHVLKAR
                     RMEARHLASGARASAAGTIAIVDNGPRLELQGEWSDFRWPLTGRAPAVRSAAGSFTLA
                     GVLPYQVHLRGRGRAAELPEMTVEASGTLGKDGVTFDPAAVDLFGGHALLSGRVLWSP
                     AESWSVSGRATGINPGELRADLPGSVSFTLTATGRGFDSRGEMSASFSEIGGRLRGLA
                     ASGGGTLTRSGTSWSFKEVRVGLGGASFALDGRLAERVDLRFAANVRDLSLLAANAHG
                     ELKASGAVSGTLAEPAIVASAHATDLAWQGATLRAFDAEVNFNPAASAQESNIDARLR
                     ELSFAGRTLSTVVFTLTGPPSAYNLHLAATATGLAAGAQAVGAYSRGVFNGTLTALSV
                     TGSEQLRLSLEHPVDLSLALDHARIAWLCLIGTPGSVCEDARWTPEEWAATVSANELP
                     LQTLTAGMTPKVDYQGTVSAFLQLTGHANRPVQGMLTAQLADAEIVHRLVSHKVEHTR
                     VGSGTVEVTATADLVTASADLGDATVGTVHATLELQRTAPSWQQMPLSGELHAQTAEA
                     SLVTLYVPDIDRAAGHLS"
BASE COUNT          332 a          777 c          821 g          345 t
ORIGIN      
        1 cgctcagatg gccggcggcc cggtcgatgt ccggcacgta aagcgtcacc aggctggcct
       61 cggccgtctg cgcgtgcagc tccccgctca agggcatctg ctgccacgag ggggccgtgc
      121 gttgcagctc gagcgttgcg tgcacggtgc cgaccgtcgc atctccgagg tcggcgctcg
      181 cagtgacgag atcggcggtc gccgtcacct ccacggtgcc ggagccgaca cgcgtgtgct
      241 ccactttgtg gctgacgagc cggtgcacga tttcggcgtc ggcgagctgc gccgtgagca
      301 taccctgcac gggcctgttg gcatggccgg tgagctgcag gaacgcgctc accgttccct
      361 ggtaatcgac cttgggtgtc atcccggccg tgagggtctg gagcggcagc tcattggccg
      421 agaccgtggc ggcccattct tccggggtcc agcgggcgtc ctcgcagaca ctgccgggcg
      481 tgccgatgag acacagccag gcgatgcgcg catgatcgag cgccaggctc aggtccaccg
      541 gatgctcgag cgagagtcgc agttgctcgc tgccggtcac cgacaaggcg gtgagcgtgc
      601 cgttgaacac cccgcgggag taggcgccga ccgcctgtgc gcccgctgcg agtccggtgg
      661 cggtggcggc gaggtgcaga ttgtaggccg aggggggccc ggtcagcgtg aacaccaccg
      721 tgctgagcgt gcggccggcg aagctcagct cgcgcagccg cgcatcgata ttggattcct
      781 gcgcactcgc ggccggattg aagttcacct cggcgtcgaa tgccctgagg gtcgcgccct
      841 gccaggcgag atcggtagca tgcgcgctcg ccacgatggc gggctcggcg agagtcccgc
      901 tcaccgctcc cgaggccttg agctcgccgt gcgcattcgc cgcgagcaga ctgaggtcgc
      961 gcacgttggc cgcgaagcgc aggtcgacgc gctccgccag gcggccatcg agtgcgaagc
     1021 ttgcgccgcc gagtccgacg cgcacctcct tgaagctcca gctcgtgccg ctgcgggtga
     1081 gcgtgccgcc accgctcgct gccagcccgc gcaggcggcc gccgatctcg ctgaaggaag
     1141 cgctcatctc gccgcgggaa tcgaagcccc gacccgtggc cgtgagcgtg aagctgacac
     1201 tgcccggcag gtcggcgcgt aactccccgg ggttgatgcc ggtcgcgcgt ccgctgacgg
     1261 accagctctc cgctggcgac cacagcacgc ggccgctgag gagtgcatgc ccgccgaaca
     1321 gatcgaccgc cgcgggatcg aaggtgacac cgtccttgcc gagcgtgccg ctcgcctcga
     1381 ccgtcatctc cggcagctcc gctgcccggc cgcggccacg gaggtgcacc tggtacggca
     1441 ggacacccgc cagcgtgaag gagcccgcgg cgctgcgcac ggcgggagcg cgcccggtga
     1501 gtggccagcg gaaatcgctc cattctccct gcagctcgag gcgcggaccg ttgtcgacga
     1561 tcgcgatcgt gcccgcggcg ctggcgcgcg cccccgaggc gaggtggcgc gcttccatgc
     1621 ggcgcgcctt gagcacgtgc gcagcgtagt tgccgtcgaa ccgcacctcg aacacgccgg
     1681 cgtgaagccc ggtggggtcg atgggtccat gagcctggaa gccgtgctca tcccccgagc
     1741 ccgcgaggtg cccggtcatg ctgccgagcg ggctgctcag atggaaggcg cgcagatcga
     1801 agctctgcac ggccgcatcg gcggcccagt gccagtggct ggtgagctcg agcgcctgac
     1861 cggagaggtc ggcgcggaac gggctggtga cgtgcgcgac gacgttcaag gcatcgaggt
     1921 tgccacgtgc actgccggcc agcgtccacg acggctgtcc cgccggggtc cactcgagat
     1981 gacccttgac ctccatgccg agcggatcgg tcgcgcgcag ggtgcccagc gcgttgacgt
     2041 gagcgccctc gagccgtcca tcgagcccga agaagcgaat gtagctgtgg cggatgatcg
     2101 ctgcgccgcg aatgtcgctg accgtgagct gaaaaccgtt gtagaccgtc agttgcgcgc
     2161 tgccgatctg cgcgtcctcg gcgttgatga agagccacgc gggcaagaag ctcggcggcc
     2221 cggggagcga gggtttggtg cggcgcctca cctcgatgag ggcgctgtgc acact
//
LOCUS       DATDMP010000697         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_1998195, whole genome shotgun sequence.
ACCESSION   DATDMP010000697 DATDMP010000000
VERSION     DATDMP010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998195"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="prfB"
                     /locus_tag="VI195_12150"
     CDS             <1..698
                     /gene="prfB"
                     /locus_tag="VI195_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY6825188.1"
                     /translation="GAGGTEAQDWAQMLLRMYLRWGAAHGFKCEVIDSSPGEVAGIKS
                     ATIELQGEYAYGWLRTETGVHRLVRKSPFDSGNRRHTSFASVFVSPEIDDDIEIEINP
                     ADLKMDVYRSSGAGGQHVNKTESAVRLTHLPSGIVVACQNERSQHKNRSTAMKMLRAK
                     LYELEVNKRNAAARVLEESKSDVSWGNQIRSYVLDQSRIKDLRTGVEVGNTTAVLDGD
                     LDQFLEASLKAGV"
     gene            704..2245
                     /gene="lysS"
                     /locus_tag="VI195_12155"
     CDS             704..2245
                     /gene="lysS"
                     /locus_tag="VI195_12155"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY6825189.1"
                     /translation="MKQDQGGGRDGATGGNGGDGGDENRLIAERRAKLGELRKRGPAF
                     PNDFRRDSLAGQLHGSFGERSGEWLEANPTRVQVGGRLLLRRVMGKASFAKISDRTGQ
                     IQLYLQQETLGAAYEAFKGWDIGDIVGASGVLFRTKAGELSVRVDALSLITKSLRPLP
                     DKWHGLADTETRYRQRYVDLIVSESTRQVFRTRSRIVRYLRDFLDALDFLEVETPMMQ
                     PIPGGAVARPFKTHHKALGLDMYLRIAPELYLKRLIVGGLERVYEINRNFRNEGLSTQ
                     HNPEFTMLEAYLAYADYRDLMDWIEKATRGLADTLYGTQQLTYQGRPYDLSKPFRRLT
                     LEQAIIEHNPGIDPLSLRDATYLRKRCEQLGAHFEPSDGAGKLQFELFEKTVESALLD
                     PTFVHGYPVEVSPLSRPNDADPFIADRFEFFLAGREIANGFSELNDPEEQAARFRIQA
                     ARKERGDEEAMFFDADYVRALEYGMPPAAGLGVGIDRLVMFFTDSPSIRDVILFPHMR
                     PEAPA"
BASE COUNT          441 a          818 c          808 g          423 t
ORIGIN      
        1 caggagccgg cggtaccgag gcgcaggact gggcgcagat gctgctgcgc atgtacctgc
       61 gctggggtgc ggcgcacggc ttcaaatgcg aagtgatcga ttccagtccc ggcgaggtcg
      121 ccggcatcaa gagtgcgacc atcgagctgc agggcgagta tgcctacggc tggctgcgta
      181 ccgagaccgg cgtgcaccgc ctggtgcgca agtccccgtt cgattccggc aatcgccgcc
      241 acacctcgtt cgcgtcggtg ttcgtctcgc ccgagatcga cgatgacatc gagatcgaga
      301 tcaacccggc ggacctcaag atggacgtgt accgctcctc gggcgcaggc ggacagcacg
      361 tcaacaagac cgagtccgcc gtgcgcctca cgcacttgcc gagcggcatc gtggtcgcct
      421 gccagaacga gcgcagccag cacaagaatc gctcgacggc gatgaagatg ctgcgggcga
      481 agctgtacga gctcgaggtc aacaagcgca acgccgccgc ccgggtgctg gaggagtcga
      541 aatcggatgt gagctggggc aatcagatcc gctcgtacgt tctcgatcag tcgcgcatca
      601 aggacctgcg caccggagtg gaagtaggca acaccaccgc agtactcgat ggagatctgg
      661 atcagtttct ggaggcgtcg ctgaaggcgg gtgtttgagc ggcatgaagc aagatcaggg
      721 tggtgggcgc gacggcgcaa cgggtggcaa cggcggcgat ggcggcgatg agaaccggct
      781 gatcgccgag cggcgcgcta agctcggcga gctgcgcaag cgcggcccgg ctttccccaa
      841 cgattttcgt cgcgactcac tcgcggggca gctgcacggc tccttcggtg agcgcagcgg
      901 cgagtggctc gaggccaacc cgacgcgtgt acaggtggga ggccgcctgc tgctccgccg
      961 ggtgatgggc aaggcgagct tcgcgaagat cagcgaccgc accgggcaga tccagctgta
     1021 cctgcagcag gagactctcg gcgcggctta cgaagccttc aagggctggg acatcggcga
     1081 catcgtcggc gcctccggcg tgctgttccg taccaaggcc ggggagctct cggtgcgggt
     1141 cgatgcactc tcgctcatta ccaaatccct gcggccgctg ccggacaagt ggcacggcct
     1201 cgcggacacc gagacgcgct accgccagcg ttatgtcgat ctcatcgtga gcgagtcgac
     1261 acgccaggtg ttccgtaccc gcagccgcat cgtgcgctac ctgcgcgact tcctcgacgc
     1321 gctggatttt ctcgaggtcg agaccccgat gatgcagccg attcccggcg gggcggtggc
     1381 gcgcccattc aagacgcatc acaaggccct cggcctggac atgtacctgc gcatcgcgcc
     1441 cgagctgtat ctgaagcggc tgatcgtcgg cgggctcgag cgcgtctacg agatcaaccg
     1501 caacttccgc aacgaggggc tctcgacgca gcacaatccc gagttcacga tgctcgaggc
     1561 gtacctcgcg tacgccgatt accgcgatct catggactgg atcgagaagg cgacccgcgg
     1621 cctcgccgat acgctctacg ggactcagca gctcacctac cagggccgcc cctacgatct
     1681 gagcaaaccc ttccgccggc tcacgctgga gcaggccatc atcgagcaca atcccggcat
     1741 cgatccgctg tcgctgcgcg atgccaccta cttgcgcaag cgctgcgagc agctcggcgc
     1801 gcacttcgag cccagcgacg gagcgggcaa gctgcagttc gagctgttcg agaagaccgt
     1861 ggaaagtgcg ctgctcgatc cgaccttcgt ccatgggtat ccggtggagg tctcgccgct
     1921 ctcgagaccc aatgatgccg acccgttcat cgcggatcgt ttcgagttct tccttgccgg
     1981 ccgcgagatc gccaacggct tttccgagct caacgatccg gaggaacagg ccgcgcgctt
     2041 tcgcatccag gcggcacgca aggaacgcgg cgacgaggag gcgatgttct tcgatgccga
     2101 ttacgtgcgt gcgctcgagt acggcatgcc tcccgctgca ggtctcggcg tcggcatcga
     2161 ccgcctggtg atgttcttca ccgactcgcc atcgatccgc gacgtcattc tcttcccgca
     2221 catgcgcccc gaggcgccgg cgtgatcaat gcgtgcggcg ggggctccgg cgtcatgagt
     2281 gcatgcggcg gggctccggc gtcatgactg ccttccagcc atagttcacc ttttggtgaa
     2341 ctattcctgc aacgtgtatc taccgcgcct acccgatcaa gccgcgcgca ccccgtcgag
     2401 cctactccgg tagcgcatag ggcagctcga gctgctccgc ctcgagggcc gctgtgtcaa
     2461 agcgacctgc gaccgccgcg gtttcggccg
//
LOCUS       DATDMP010000698         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_665567, whole genome shotgun sequence.
ACCESSION   DATDMP010000698 DATDMP010000000
VERSION     DATDMP010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665567"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VI195_12160"
     CDS             complement(<1..432)
                     /locus_tag="VI195_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6825190.1"
                     /translation="MSTHSIDVAAVRAATPGCTEVIHFNNAGAALPPKVVTDTVLAYT
                     AREALIGGYEAQEAAAAELASSYDALALLLGARPRQIALIENATRAWDMAFYGVPLGR
                     GDRILTCRSEYASNYLAYLQVSRRRGVRIDVIPDDADGALDP"
     gene            complement(539..1441)
                     /locus_tag="VI195_12165"
     CDS             complement(539..1441)
                     /locus_tag="VI195_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEY6825191.1"
                     /translation="MPKTYNLDRLERYVKPFRITKDKGFRLKDFDPADTRDLRMEKKE
                     AAELLQRGTEWLAREQELLYAQDRWSLLLIFQAMDAAGKDSTIKHVMSGINPQGCQVC
                     SFKTPSAEELDHDFLWRHTKCLPERGHIGIFNRSYDEEVLVVRVHPDILEQQKLPAAC
                     VSKDIWTQRLADIARFEQYLTRQGTIILKFFLHVSRAEQKKRFLDRLDNPQKHWKFEA
                     ADVRERAYWSDYQSAYQEAIRATASQQAPWYVVPADNKWFTRLVVAAAIVKALDELDL
                     SYPRISPKKMRELRATRAMLARKR"
     gene            complement(1526..>2054)
                     /locus_tag="VI195_12170"
     CDS             complement(1526..>2054)
                     /locus_tag="VI195_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825192.1"
                     /translation="YAIYTPATSPANLASVFAAGFGTDYLVNNNYRASYLNDALMHPD
                     GGFPTVTDGLPPADPGIALRMQLKVNDLRNWTPTAPTLLCGGNSDPTVFFFNTTLMQH
                     YWTLHPPAVAPVILDVDSSPGSNDPYATLKTEFGVAKDLIELQGGDSEVLAAYHATLV
                     PPFCLSAVKSFFDAH"
BASE COUNT          349 a          625 c          689 g          391 t
ORIGIN      
        1 cgggtccagc gcgccgtcgg cgtcatcggg aatcacatcg atgcgcacgc cgcggcggcg
       61 gctcacttgc aggtaggcca gataattgct cgcgtactcc gagcgacacg tgaggatgcg
      121 atcgccgcgc ccgagcggaa ccccgtaaaa cgccatgtcc caggcgcggg tggcgttttc
      181 gatcaatgcg atttgccggg ggcgcgcgcc gagcagcaga gcgagcgcat cgtagctgga
      241 ggcgagctcc gcggccgccg cctcctgggc ctcgtagcct ccgatcagcg cttcgcgcgc
      301 cgtgtaggcg agcaccgtgt cggtgaccac cttgggtggc agcgctgcgc ccgcgttgtt
      361 gaagtgaatg acctcggtgc acccgggggt ggccgcccgg accgctgcga catcgatgct
      421 gtgggtcgac atctgcctta ccgccttgac gaggtcacgc cagtattttc cgccagtgcg
      481 cgtgggaggg aaggccacgc gcggtcgagc tcacgctcgc ggcccgccgg ccgcgctctc
      541 agcgtttcct ggcaagcatg gcgcgcgtcg cgcgcaactc ccgcattttt ttcgggctga
      601 tgcgggggta ggagaggtcg agctcatcga gcgccttcac gatcgccgcc gcgaccacca
      661 gccgcgtaaa ccacttgttg tccgccggca ccacgtacca gggcgcctgc tgcgaagcgg
      721 tagcgcgtat cgcttcctgg tacgcggact ggtagtcgga ccagtaggcc cgctcgcgca
      781 catctgcggc ctcgaatttc cagtgcttct gcgggttgtc gagccgatcg agaaaacgct
      841 ttttctgctc ggcgcgcgac acgtgcagaa agaacttcag gatgatcgtc ccctgacgcg
      901 tcaggtattg ctcgaagcgc gcgatatcgg cgaggcgctg cgtccagatg tccttgctca
      961 cgcaggctgc agggagcttt tgttgctcga ggatgtccgg gtgcacgcgc accaccagca
     1021 cctcctcgtc ataggagcgg ttgaagatgc cgatatgtcc gcgctcgggc aggcacttgg
     1081 tgtggcgcca gagaaagtcg tggtccagct cttctgcaga gggcgtcttg aacgagcaca
     1141 cctggcaacc ctgcggattg atgcccgaca tcacatgctt gatcgtgctg tccttgcctg
     1201 ctgcatccat tgcctgaaag atgagtaaca gcgaccagcg gtcctgggcg taaagcagct
     1261 cctgctcccg cgcgagccat tcggtcccgc gttgcagcag ctcggcggcc tccttcttct
     1321 ccatccgcag atcgcgagta tcggcggggt cgaaatcttt cagccggaat cctttgtctt
     1381 tggtgatgcg gaagggtttc acgtaccgct cgaggcgatc gaggttgtag gtttttggca
     1441 tgacggttgc tttgcgtggc tggccaacgg cgcaagctag gcgaggcctc tgcctcgcgc
     1501 aacctaccgc gctgggcaaa ctcaatcagt gcgcgtcaaa gaacgatttg acggcactca
     1561 ggcagaaggg cggcacgagt gtggcgtggt aggcggccag cacctctgag tccccgccct
     1621 gcagctcgat caggtccttg gcaacgccga attcggtctt gagcgtcgcg tagggatcgt
     1681 tggagcccgg tgaggaatcg acgtcgagaa tgaccggagc caccgccggc gggtgcagcg
     1741 tccaatagtg ctgcatgagc gtggtgttga agaaaaacac ggtgggatcg gagttgcccc
     1801 cgcacagcag ggtgggagcg gtcggcgtcc agttgcgcag gtcgttgacc ttcagctgca
     1861 tgcgcagcgc gatgcccgga tcggcgggcg gcaggccatc ggtaaccgtc gggaagccac
     1921 catcggggtg catgagcgcg tcattgaggt agctggcgcg atagttgttg ttgacgagat
     1981 agtccgtgcc gaaccccgcg gcaaacacac tcgcgagatt ggccggtgag gtcgccggcg
     2041 tatagatcgc atag
//
LOCUS       DATDMP010000699         2711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_887942, whole genome shotgun sequence.
ACCESSION   DATDMP010000699 DATDMP010000000
VERSION     DATDMP010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887942"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VI195_12175"
     CDS             complement(<1..740)
                     /locus_tag="VI195_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825193.1"
                     /translation="MPKRSDIHSILIIGAGPIVIGQACEFDYSGAQACKALREEGFRV
                     ILVNSNPATIMTDPEMADAIYIEPVNAHTLARIIEKERPDALLPTMGGQTGLNTALDL
                     VREGVLEKYGVELIAASRAAIDMAEDRELFRNAMREIGLETPQSQIARSHDEALAIAG
                     EIGYPVVIRPSFTLGGSGGGIAYNREELEAIVARGLDASPTGEVLLEESVIGWKEFEM
                     EVVRDRKDNCIIVCSIENLDPMGVHTGD"
     gene            complement(744..1928)
                     /gene="carA"
                     /locus_tag="VI195_12180"
     CDS             complement(744..1928)
                     /gene="carA"
                     /locus_tag="VI195_12180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY6825194.1"
                     /translation="MSVPAVLALADGTIFRGRSIGAKGNTTGEVVFNTALTGYQEILT
                     DPSYARQIVTLTVPHIGNTGATPEDLEAAQVYPAGLVIRDLPLLSSNWRANESLTEFL
                     ERGKMVAIAGIDTRKLTRLLREKGAQAGCIMTGEQLDESAALKIARKFPGLKGMDLAK
                     VVSVKRSYQWNEGTLWPEPERAPIRASQRLHVVAYDFGIKRNILRLLADGGCRMTVVP
                     AQTSAEDALALEPDGIFLSNGPGDPEPCSYAIKATQRFLETDLPVFGICLGHQILGLA
                     AGARTVKMKFGHHGANHPVQELESGRVFITSQNHGFAVDESTLPAGVVATHRSLFDGS
                     LQGLAFQERPVFGFQGHPEASPGPHDLRPLFERFNHLMLRRLQAQLKVAEENLRKQAG
                     GR"
     gene            complement(2118..>2711)
                     /gene="dapB"
                     /locus_tag="VI195_12185"
     CDS             complement(2118..>2711)
                     /gene="dapB"
                     /locus_tag="VI195_12185"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY6825195.1"
                     /translation="VALDFSQAAAARGTLDACRAARKPLLLGTTGFGAQLDAQLAAAA
                     REVALLVDPNTSLGVALLTELTRAAAQALPASFDIDVLELHHRLKQDAPSGTARALVQ
                     VAAEARGLAAPNTAGGRSPGPRPDGEIGLASLRAGDIVGEHTVLFCGAGEELALTHRA
                     HDRGIFARGALSAALWLSSCPPGRYGMGDFLRLKTGA"
BASE COUNT          435 a          890 c          912 g          474 t
ORIGIN      
        1 ctgtcgccgg tgtgcacgcc catcggatcg aggttctcga tcgagcaaac gatgatgcag
       61 ttgtccttgc ggtcccgcac cacctccatc tcgaactctt tccagccgat caccgattcc
      121 tcgagcagca cctcgccggt cggcgaggca tcgaggccgc gtgccacgat ggcctcgagc
      181 tcctcgcggt tgtaggcgat gccgccgccg ctgcccccga gggtgaagga cggcctgatc
      241 accaccgggt agccgatctc gccggcgatg gcgagtgctt catcgtgact gcgcgcgatc
      301 tgcgactgcg gcgtctccag gccgatctcg cgcatggcgt tgcggaacag ctcccggtct
      361 tccgccatgt cgatcgcggc gcgtgaagcg gcgatcagct ccacgccgta tttctccagc
      421 acgccctcac gcaccagatc gagcgcggtg ttgagcccgg tctgtccgcc catggtcggc
      481 agcagcgcat ccggacgctc tttctcgatg atgcgtgcga gggtgtgcgc gttcaccggc
      541 tcgatgtaga tggcatcggc catctccgga tcggtcatga tggtcgcggg attcgaattg
      601 accaggatga cgcggaagcc ctcctcgcgc agcgccttgc acgcctgcgc gccggagtaa
      661 tcgaactcgc acgcctgtcc gatgacgatg ggaccggcac cgatgatgag gatgctgtgg
      721 atgtcggagc gcttcggcac tgctcagcgt ccgcccgcct gcttgcgcag gttctcctcg
      781 gccaccttca gctgcgcctg cagccgccgc agcatcaggt gattgaagcg ctcgaacagc
      841 gggcgcagat cgtgcgggcc ggggctcgcc tccgggtgcc cctggaagcc gaacaccgga
      901 cgctcctgga acgcgagtcc ctggagcgag ccatcgaaga gcgagcggtg ggtcgcgacg
      961 actcccgcgg gcagcgtgct ctcatccacg gcaaagccgt ggttctgact cgtgatgaac
     1021 acgcggccgg actcgagctc ctgcacgggg tgattggcgc cgtgatggcc gaacttcatc
     1081 ttcacggtgc gcgcaccggc ggcgagcccg agtatctgat gcccgagaca gatgccgaac
     1141 acgggcaggt cggtctcgag gaagcgctgc gtggccttga tggcataact gcagggttcg
     1201 ggatcgcccg gtccgttgga gaggaagatc ccatcgggct ccagagcgag cgcgtcctcg
     1261 gcggaagttt gcgccggcac caccgtcatg cggcagccgc catcggcaag aagcctgagg
     1321 atattgcgct tgatgccgaa gtcgtaggcc accacgtgca ggcgttgact cgcgcggatc
     1381 ggcgcgcgct cgggctcggg ccagagcgtg ccttcgttcc attgatagct gcgcttgaca
     1441 ctcaccacct tggcaaggtc catgcccttg aggcccggga acttgcgcgc gatcttcagt
     1501 gccgcgctct catcgagctg ctcgccggtc atgatgcagc ccgcctgcgc gcccttctcg
     1561 cgcagcagcc gggtgagctt gcgcgtatcg atgcccgcga ttgcgaccat cttgccgcgc
     1621 tcgaggaact cggtgagcga ctcgttggcg cgccagttgc tcgagagcag cggcagatcg
     1681 cggatcacga gacccgccgg atacacctgc gccgcttcga gatcctcggg cgtcgctccg
     1741 gtgttgccga tgtgcggaac ggtgagggtg acgatctgac gcgcgtagga agggtcggtg
     1801 agaatttcct gataacccgt gagggcggtg ttgaaaacga cttcgcctgt ggtgtttccc
     1861 ttggctccga tcgaccggcc gcggaagatc gttccatcgg ccagcgcgag caccgcaggt
     1921 acgctcacgt gatcacttct ccctgacgca gatacacaaa gcgggaggag ttctcatcga
     1981 acatcctccc gccttgcatg gactaacccg tacacggatt aggccgaaat tttaggggtt
     2041 tttcgggcgg tgtgtccacg ggaaattccg ccttccgtgg ccggatactg gcttctcgtg
     2101 gtgtcacgga caatgttcta agcccctgtt ttcagtcgga gaaagtcacc catcccgtag
     2161 cgccccgggg ggcatgacga gagccagagc gcagccgaga gcgcgccgcg ggcgaagatg
     2221 ccccgatcgt gggcgcggtg cgtcagcgcg agctcctcgc ccgccccgca aaagagcacc
     2281 gtgtgctccc cgacgatgtc gcccgcacgg agcgaggcga ggccgatctc accgtcgggg
     2341 cgcgggcccg ggctgcgtcc gcccgccgta ttaggggcag cgagtccgcg ggcctcggcc
     2401 gccacctgca cgagcgcacg cgcggtgccc gagggagcgt cttgcttgag gcggtgatgg
     2461 agctccagca cgtcgatgtc gaagctcgca ggcaacgctt gggccgcggc gcgcgtgagc
     2521 tcggtgagca gtgcgacccc caggctggta ttcgggtcga cgagcagcgc cacctcgcgc
     2581 gcggcggctg cgagctgcgc atcgagctgc gcgccaaaac cggtggtacc gagcagcagc
     2641 ggcttgcgtg ccgcccggca ggcatcgagg gtgccgcgcg ctgcggccgc ctgactgaaa
     2701 tcgagcgcga c
//
LOCUS       DATDMP010000700         3761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_998346, whole genome shotgun sequence.
ACCESSION   DATDMP010000700 DATDMP010000000
VERSION     DATDMP010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998346"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VI195_12190"
     CDS             <1..1077
                     /locus_tag="VI195_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY6825196.1"
                     /translation="PAPAPASASAAAAGAGTAAGSGESSAPVANPTAPMHYTVKRGDT
                     LWGIASMYLKDPWLWPEVWIINPQVPNPHLIYPGDKLALAYGAGGRLEVRLEEAGVSR
                     LDPRLRSDPLANAIPTIPYSAIAAFLSRPTIMTSDEIRHAPYVLAFRDQHQVAGNEIE
                     VYVRNLQATENSRFAVMHVATPLVDPDDGKVVGYEGIYTATALVKRSGEPAKAVLIDL
                     ARETLAGDRLMSSDNEVPLNFTPAAPSSEVHGRIIDVVGGTDLVGQFYVVAINRGKRH
                     GIGPGTVLAVDQEGEVVRDLYGGGRSIANNMNGFNTQFVPHVKLPNERAGTLLVFKSY
                     DRISYGLIVGASNIIHIGAPVRNP"
     gene            1182..2273
                     /gene="dprA"
                     /locus_tag="VI195_12195"
     CDS             1182..2273
                     /gene="dprA"
                     /locus_tag="VI195_12195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY6825197.1"
                     /translation="METSRARAVLARTPDLDAGQLRDLLAAAGGEPERALGARVTQRM
                     ELPSAAQQFLASPDEVAVDADLTWLSSRGVHIVLASDPEYPPLLRQMPGAPAALYVEG
                     SVAALSSPQIAIVGSRSPTPAGRSTAREFAAYFAGLGLTVTSGLAVGIDAASHEGALA
                     GGGATIAVYATGLDRVYPAEHAALAQRIRACGALVSEYPPRTPPLRGHFPRRNRIIAG
                     LSLGTLVVEAARYSGSLTTARHAVDAGREVFAIPGSIHSPLSRGCHQLIKSGAKLVEE
                     AADVMSELPFSVRKEAVTAPQPEPDKPRELDKEYEMLLDALGFEPATIDVLVARTHLP
                     GESVASMLLILELEGRVAALPGGRYGRLP"
     gene            2275..2760
                     /locus_tag="VI195_12200"
     CDS             2275..2760
                     /locus_tag="VI195_12200"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY6825198.1"
                     /translation="MTTGSVLDILIYVFDRYMLIDMPAVPRREHLARDLERAGFARAK
                     VERALDWLTDLAFHQDPAAPAQTRAEVQGVRVFTDGELMRLSTQCRGLLLTLERAQVL
                     TAQQRELVIERMLALDADDPDTEQLKWVVLMVLSSQPGCELAVERLGGLMRAASDSAP
                     H"
     gene            2841..>3761
                     /locus_tag="VI195_12205"
     CDS             2841..>3761
                     /locus_tag="VI195_12205"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEY6825199.1"
                     /translation="MADNLVIVESPAKARTIKKYLGRDFEVLASYGHVRDLVPKEGAV
                     DPDKNYAMKYQVLEKNERHLAAIARALGKSNALYLATDPDREGEAIAWHLKEILDSRG
                     NLNGKHVHRVVFYEITRNAIRAAVERPRDLSMELVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRMICEREEEIAAFIAQEYWTIEGEGAHGGQAFPLKLLEYRGE
                     KVEQFSFTSERTAREVENTLRAAAGASGGAPGTLQVLAIDRKQRRRNPAPPFTTSTLQ
                     QEAARKLGFNARRTMRLAQQLYEGQDIGDGN"
BASE COUNT          621 a         1268 c         1249 g          623 t
ORIGIN      
        1 ccggcgccgg cgcccgcatc cgcgagcgcg gcggcagcag gcgcgggcac tgcggccggc
       61 tccggcgaga gctcggctcc ggtcgccaat cccaccgcgc ccatgcacta cacggtcaag
      121 cgcggcgata ccctgtgggg catcgcctcg atgtatctga aggacccgtg gctgtggcct
      181 gaggtctgga tcatcaatcc ccaggtaccc aatccgcacc tgatctatcc gggcgacaag
      241 ctcgcactcg cctatggcgc gggcggccgc ctggaagtgc gtctggaaga ggcgggcgta
      301 tcgcggctcg acccgcggct gcgcagcgat ccgcttgcca acgccattcc caccattccg
      361 tattcggcga tcgcggcgtt cctgtcgaga ccgaccatca tgacctcgga cgagatccgc
      421 cacgcgcctt atgtgctcgc gtttcgcgac cagcatcagg tggccggtaa cgagatcgag
      481 gtgtacgtcc gcaaccttca ggcgactgag aattcgcgtt tcgcggtcat gcacgtcgca
      541 acaccgttgg tcgaccccga cgatggcaag gtcgtcggct acgagggcat ctacaccgcg
      601 accgctctcg tgaagcgctc cggtgagccc gcgaaggcgg tactgatcga cctggcgcgc
      661 gagacgctcg ccggtgatcg gctgatgtca tcggacaacg aggtgccgct gaacttcact
      721 ccggctgcgc caagctccga ggtgcacggc cggatcatcg atgtggtcgg ggggaccgac
      781 ctcgtcggac agttctacgt cgtcgccatc aaccgtggca agcgccacgg catcgggccg
      841 ggcaccgtac tcgccgtcga ccaggagggc gaggtggtgc gcgatctgta tggcggtggc
      901 cgttccatcg ccaacaatat gaacggcttc aacacccaat tcgtgccgca tgtgaagctg
      961 ccgaacgagc gggcgggcac gctgctggtg ttcaagagct acgatcgaat cagttatggc
     1021 ctgatcgtgg gcgcttccaa catcatccac atcggcgccc cggtccgtaa cccgtaacgc
     1081 ctgagctgct gccgccgccg gcatcgccgg ctggcggtgc ctgcacttgc gtgccatttg
     1141 cctgcggctc gcgccgcgcg cttcctttat gctcgccagc gatggagacg agccgagccc
     1201 gcgccgtgtt ggcacgcacc cctgacctcg acgccgggca gctgcgcgat ctgctcgcgg
     1261 ccgcgggcgg tgagccggaa cgtgcactcg gcgcacgggt gacgcagcgg atggagctgc
     1321 cgtccgccgc acagcagttt ctcgcctccc cggatgaggt ggcggtcgat gccgacctca
     1381 cctggctctc ctcgcgcgga gtgcacatcg tgctcgcctc cgatcccgag tatccgccgc
     1441 tgctgcggca gatgccgggc gcgcccgccg cgctctatgt ggaggggtcg gtggcggcac
     1501 tctcctcacc acagatcgcc atcgtcggct cgcgcagccc cacgccggcc ggacgctcga
     1561 ccgcgcgcga gttcgctgcg tacttcgcag gccttgggct cacggtcacc agcgggctcg
     1621 cggtgggcat cgatgccgcg agtcacgaag gcgcacttgc cggaggtgga gcgaccatcg
     1681 cggtctatgc caccgggctc gatcgcgtct accccgccga gcacgcggca ctggcgcagc
     1741 gcatccgcgc atgcggtgcg ttggtatcgg agtacccgcc gcggacgccg ccgctgcgcg
     1801 ggcatttccc gcgccgcaat cgcatcatcg ccgggctttc actcggcacg ctcgtcgtgg
     1861 aggcggcgcg ctacagcggc tcgctgacca ccgcgcgcca cgcggtggac gcagggcggg
     1921 aggtgtttgc catccccgga tcgattcaca gtccgctctc gcgcggttgc caccaactga
     1981 tcaagagcgg cgccaagctg gtcgaggaag cggccgacgt gatgtcagag ttgccttttt
     2041 ccgtgcggaa agaagcagtt acggcgccac aacccgagcc ggacaagccg cgggaattgg
     2101 acaaggagta tgaaatgctg ttagatgccc tcggctttga acccgcgacc atcgatgttc
     2161 tcgtcgcgcg cacgcacctg ccgggtgaat cagttgcctc gatgctgctg atactcgagc
     2221 tcgaggggcg tgtggcggcg ctcccgggcg gccgttacgg ccggttacct tgagatgacc
     2281 actggcagcg ttctcgacat cctgatctac gtcttcgacc gctacatgct catcgacatg
     2341 ccggcggtac cgcggcgcga gcatctggcg cgcgatctcg agcgcgccgg tttcgcgcgc
     2401 gccaaggtcg agcgcgcact cgattggctg accgatctcg cgttccatca ggatcccgcg
     2461 gctccggcgc agacgcgtgc cgaggtgcag ggcgtgcgcg tgttcaccga cggcgagctc
     2521 atgcgcctgt cgacgcaatg tcgcgggctg ctgttgacgc tcgagcgcgc acaggtactg
     2581 acggcacagc agcgcgagct ggtgatagag cgcatgctcg cgctcgatgc ggacgaccct
     2641 gacaccgagc agctcaaatg ggtggtgctc atggtgctgt ccagccagcc tggctgtgag
     2701 ctcgcggtcg agcggctcgg cgggctgatg cgggcagcat cggacagtgc gccgcattga
     2761 gcgcacgact ggtgcgggtt gagaggtccg cggctcgccc gcggtttttt gtttcaggat
     2821 cgagcgtttc ccgataggtc atggcagaca acctcgtcat cgtcgagtcc cccgccaagg
     2881 caaggaccat aaagaagtat ctgggacgtg acttcgaggt gctcgcctcc tacggtcacg
     2941 tgcgcgatct cgtgcccaag gagggcgcgg tcgatcccga caagaactac gccatgaagt
     3001 atcaggtgct cgagaaaaac gagcgccatc tggcggccat tgcccgcgca ctcggcaagt
     3061 cgaacgcgct gtacctcgct accgaccccg accgcgaggg cgaggcgatc gcctggcact
     3121 tgaaggagat actcgactcg cgcggcaacc tcaacggcaa gcacgtgcac cgcgtggtgt
     3181 tctatgagat tacgcgcaac gccatccgcg cagcggttga gcgtccacgt gatctgtcga
     3241 tggagctggt caacgcacaa caggcgcgcc gcgcgctcga ttacctggtg ggcttcaatc
     3301 tctcgccact cctgtggaaa aaagtccgcc gcggcctgtc cgcgggtcgc gtgcagagcc
     3361 cggcgctgcg catgatctgc gagcgcgagg aggaaatcgc cgccttcatc gcgcaggagt
     3421 actggaccat cgagggcgag ggcgcgcacg gcggccaggc ctttccgctc aagctgctcg
     3481 aatatcgcgg ggagaaggtc gagcagttca gcttcacgag cgagcgcacg gcgcgcgagg
     3541 tcgagaacac actgcgtgcg gcagccggcg ccagcggcgg cgcgccggga acgctgcagg
     3601 tgctcgccat cgaccgcaag cagcgccgcc gcaatccggc gccgcccttc acgacctcca
     3661 cgctgcagca ggaggccgcg cgcaagctcg gcttcaatgc gcgccgcacc atgcgcctcg
     3721 cgcaacagct gtacgagggg caggacatcg gcgatggcaa c
//
LOCUS       DATDMP010000701         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U414
            k141_221525, whole genome shotgun sequence.
ACCESSION   DATDMP010000701 DATDMP010000000
VERSION     DATDMP010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962614
            Sequence Read Archive: SRR25158283
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:00:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,411
            CDSs (with protein)               :: 2,411
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221525"
                     /isolate="SMAG_U414"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /gene="groEL"
                     /locus_tag="VI195_12210"
     CDS             <1..779
                     /gene="groEL"
                     /locus_tag="VI195_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY6825200.1"
                     /translation="AMLQDIAILTGGTVISDEVGLTLEKASLNDLGEAKKVVVEKENT
                     TIIDGAGKTTDIKARIESIRQQAEEATSDYDKEKLQERVAKLSGGVAVIKVGAATEIE
                     MKEKKARVEDALHATRAAVEEGVVPGGGVALIRVQRALEKLETANEDQHVGVRILSRS
                     IEEPLRQIVDNAGEDAAVILNRVKEGKGAFGWNAATGEFGDMLDEGILDPTKVTRLAL
                     QNAASVAGLLLTTEVMVAEAPKDEEHSHAGPPGGMGGMDM"
     gene            1065..1937
                     /locus_tag="VI195_12215"
     CDS             1065..1937
                     /locus_tag="VI195_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6825201.1"
                     /translation="MGKLGYRGLMCIASVGFLALGACGREGQDEMSWARGALERNKSL
                     EVVAADQQSRTFTVRLKEGGELRTVRADQVVGSPADVALAAAARPMGNSNPSAAPETA
                     ATEDAVGHAADTANEGEPPVTAETPVPHPQPVLTSESSTGLERVAPGRVLESGPGYSI
                     TAAQTAMPAAGRMHDSASNAPLERRHEPIVCQGSRLVHIDNRNLVFDGDAVSAMDGCE
                     IHITNSHISASGVGIAVRAANVHIDNSQIEGDQASIEASQGAQVYVASSRFKGLSRRL
                     DTAAVHDLGGNVWN"
BASE COUNT          395 a          636 c          715 g          325 t
ORIGIN      
        1 aagccatgtt gcaggacatc gcgattctga cgggtggcac ggtcatctcc gacgaggttg
       61 gcctgacgtt ggagaaggcg agcctgaacg atctgggcga ggcgaagaag gtcgtggtgg
      121 agaaagagaa caccaccatc atcgatggcg ctggcaagac caccgacatc aaggcacgta
      181 tcgagtcgat ccgccagcag gccgaggagg ccacctccga ctacgacaag gagaagctgc
      241 aggagcgcgt cgccaagctt tccggtggcg tcgcggtgat caaggtgggt gccgccaccg
      301 agatcgagat gaaggagaag aaggcccgcg tcgaggacgc gctgcacgcc acgcgtgccg
      361 cggtcgaaga gggcgtggtg ccgggtggcg gcgtggcact gatccgcgtg cagcgtgcgc
      421 tcgagaagct cgagaccgcg aacgaggacc agcacgtcgg tgtccgcatc ctctcccgct
      481 cgatcgagga gccgttgcgc cagatcgtcg acaacgccgg cgaggacgcc gcggtcatcc
      541 tcaatcgtgt caaggaaggc aagggcgctt tcggctggaa cgcggccacg ggtgagttcg
      601 gagacatgct cgatgagggc atcctcgatc cgaccaaggt gacccgtctc gcgctgcaga
      661 acgccgcttc ggtggcggga ctgctgctca cgacggaagt catggtggcc gaggctccga
      721 aggatgagga gcacagccat gcgggcccgc cgggcggcat gggcggaatg gatatgtaat
      781 taccctctct aaagaggtat cgattccaga aaggacactc gataggcttt acataacagt
      841 tacgggttgg cgccaggtct ctgccgggcg tcaatccgga aagacgcgga acaagacccc
      901 ctattccgcg cattcctcga ggccccacac tgtggggcct gtttttttgt gcgcgcctgt
      961 tgggtcgaca ctccgcgacc gcgttcacag catgcgcgca acgcggcgct gcagcacttc
     1021 cttacagccc agccggggtt tatcgtccaa gtgaggaggc tcccatgggc aagcttggct
     1081 atcgcggcct gatgtgtatc gccagcgtcg gctttctggc gctcggcgcc tgcggtcgcg
     1141 agggccagga tgagatgagc tgggcccgag gggccctcga gcgcaacaaa tcgctcgagg
     1201 tcgtggccgc ggaccagcag agtcgcacct ttacggtgcg gctgaaggag ggcggtgagc
     1261 tgcgtaccgt gcgcgcggat caggtggtcg gctcgccggc tgacgtagcg ctcgccgctg
     1321 cggcgcgccc gatggggaac agcaatccct ctgccgcacc cgagaccgcg gccaccgagg
     1381 acgcagtggg gcacgcggcg gacacggcga atgaagggga accgcctgtc acggctgaaa
     1441 cgccggtgcc acatccgcag cctgtgctga cctcggagag ttccaccggt ctcgagcgcg
     1501 tagcgcccgg gcgcgtgctc gagtccgggc ccggttacag catcaccgct gcgcagacgg
     1561 cgatgcccgc agccgggcgc atgcatgact ccgcgagcaa cgcaccactc gagcgccgcc
     1621 acgagccgat cgtgtgccag ggctcacggt tggtgcacat cgacaaccgc aatctggtgt
     1681 tcgatggcga tgcggtgagc gccatggatg gttgcgagat tcacatcacc aacagtcaca
     1741 tcagcgcaag cggcgtcggc atcgcggtgc gcgccgccaa cgtacacatc gacaacagcc
     1801 agatcgaagg tgatcaggcc tccatcgaag cctcccaggg cgctcaggtg tacgtggcct
     1861 cctcgcgctt caagggtctg agccgccgac tcgatacggc ggcggtgcac gatctcgggg
     1921 gcaatgtctg gaactgaagc gaccccccgg gagatgaggc ccctgcgtcc gcctacgggc
     1981 gacgcgtgcg cggatcccat agctgatccc agggtgcgcc atgagctatg cgcatggggt
     2041 atgcgcatag gacatgcgcg tgaggtatgc g
//
